LOCUS       DAPBAB010000001       137394 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPBAB010000001 DAPBAB010000000
VERSION     DAPBAB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 137394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 137394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..137394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.001"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            212..604
                     /locus_tag="RD444_000001"
     CDS             212..604
                     /locus_tag="RD444_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7547413.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="RD444_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="RD444_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDT7547414.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="RD444_000003"
     CDS             3165..4775
                     /locus_tag="RD444_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDT7547415.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="RD444_000004"
     CDS             5067..5936
                     /locus_tag="RD444_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDT7547416.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="RD444_000005"
     CDS             6101..7372
                     /locus_tag="RD444_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDT7547417.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="RD444_000006"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="RD444_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDT7547418.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="RD444_000007"
     CDS             8763..9026
                     /locus_tag="RD444_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDT7547419.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="RD444_000008"
     CDS             9471..9920
                     /locus_tag="RD444_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7547420.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="RD444_000009"
     CDS             9977..11395
                     /locus_tag="RD444_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7547421.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="RD444_000010"
     CDS             11395..12255
                     /locus_tag="RD444_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDT7547422.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="RD444_000011"
     CDS             12429..13202
                     /locus_tag="RD444_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7547423.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="RD444_000012"
     CDS             13349..13714
                     /locus_tag="RD444_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDT7547424.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="RD444_000013"
     CDS             complement(13862..15070)
                     /locus_tag="RD444_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7547425.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="RD444_000014"
     CDS             complement(15089..15367)
                     /locus_tag="RD444_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547426.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="RD444_000015"
     CDS             15521..16216
                     /locus_tag="RD444_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7547427.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="RD444_000016"
     CDS             complement(16658..17455)
                     /locus_tag="RD444_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDT7547428.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="RD444_000017"
     CDS             complement(17452..18636)
                     /locus_tag="RD444_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDT7547429.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="RD444_000018"
     CDS             complement(18661..19908)
                     /locus_tag="RD444_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDT7547430.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="RD444_000019"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="RD444_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDT7547431.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="RD444_000020"
     CDS             21888..22202
                     /locus_tag="RD444_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDT7547432.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="RD444_000021"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="RD444_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDT7547433.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="RD444_000022"
     CDS             22887..23531
                     /locus_tag="RD444_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDT7547434.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="RD444_000023"
     CDS             23546..23875
                     /locus_tag="RD444_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDT7547435.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="RD444_000024"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="RD444_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDT7547436.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="RD444_000025"
     CDS             24863..25687
                     /locus_tag="RD444_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDT7547437.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="RD444_000026"
     CDS             25680..26027
                     /locus_tag="RD444_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDT7547438.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="RD444_000027"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="RD444_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDT7547439.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="RD444_000028"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="RD444_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDT7547440.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD444_000029"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD444_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDT7547441.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD444_000030"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD444_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDT7547442.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="RD444_000031"
     CDS             31657..32451
                     /locus_tag="RD444_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547443.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="RD444_000032"
     CDS             32441..34483
                     /locus_tag="RD444_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547444.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="RD444_000033"
     CDS             34764..34913
                     /locus_tag="RD444_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDT7547445.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="RD444_000034"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="RD444_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDT7547446.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="RD444_000035"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="RD444_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDT7547447.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="RD444_000036"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="RD444_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDT7547448.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="RD444_000037"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="RD444_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDT7547449.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="RD444_000038"
     CDS             39330..40412
                     /locus_tag="RD444_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDT7547450.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="RD444_000039"
     CDS             40412..41215
                     /locus_tag="RD444_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDT7547451.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="RD444_000040"
     CDS             41285..42025
                     /locus_tag="RD444_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7547452.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="RD444_000041"
     CDS             42036..43040
                     /locus_tag="RD444_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDT7547453.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="RD444_000042"
     CDS             complement(43085..44929)
                     /locus_tag="RD444_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547454.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="RD444_000043"
     CDS             45141..45659
                     /locus_tag="RD444_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7547455.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="RD444_000044"
     CDS             45787..46470
                     /locus_tag="RD444_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDT7547456.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="RD444_000045"
     tRNA            46779..46852
                     /locus_tag="RD444_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="RD444_000046"
     CDS             47011..47370
                     /locus_tag="RD444_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDT7547457.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="RD444_000047"
     CDS             47493..49124
                     /locus_tag="RD444_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDT7547458.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="RD444_000048"
     CDS             complement(49186..49827)
                     /locus_tag="RD444_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDT7547459.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="RD444_000049"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="RD444_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDT7547460.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="RD444_000050"
     CDS             50444..50788
                     /locus_tag="RD444_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDT7547461.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="RD444_000051"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="RD444_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDT7547462.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51528..54335
                     /locus_tag="RD444_000052"
     CDS             51528..54335
                     /locus_tag="RD444_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7547463.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="RD444_000053"
     CDS             54526..55017
                     /locus_tag="RD444_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7547464.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="RD444_000054"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="RD444_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDT7547465.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="RD444_000055"
     CDS             56142..56945
                     /locus_tag="RD444_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDT7547466.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="RD444_000056"
     CDS             56966..57880
                     /locus_tag="RD444_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDT7547467.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="RD444_000057"
     CDS             57963..58337
                     /locus_tag="RD444_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDT7547468.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="RD444_000058"
     CDS             58476..58721
                     /locus_tag="RD444_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7547469.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="RD444_000059"
     CDS             58893..59957
                     /locus_tag="RD444_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDT7547470.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="RD444_000060"
     CDS             60203..60628
                     /locus_tag="RD444_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7547471.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="RD444_000061"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="RD444_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDT7547472.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="RD444_000062"
     CDS             61167..62021
                     /locus_tag="RD444_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDT7547473.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="RD444_000063"
     CDS             62022..63359
                     /locus_tag="RD444_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDT7547474.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="RD444_000064"
     CDS             63364..63588
                     /locus_tag="RD444_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDT7547475.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="RD444_000065"
     CDS             63590..64480
                     /locus_tag="RD444_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDT7547476.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="RD444_000066"
     CDS             64489..65307
                     /locus_tag="RD444_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDT7547477.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="RD444_000067"
     CDS             65414..65863
                     /locus_tag="RD444_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDT7547478.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="RD444_000068"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="RD444_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDT7547479.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="RD444_000069"
     CDS             complement(67618..67734)
                     /locus_tag="RD444_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDT7547480.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="RD444_000070"
     CDS             complement(67807..68763)
                     /locus_tag="RD444_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7547481.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="RD444_000071"
     CDS             68934..69302
                     /locus_tag="RD444_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDT7547482.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="RD444_000072"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="RD444_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDT7547483.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="RD444_000073"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="RD444_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDT7547484.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="RD444_000074"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="RD444_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDT7547485.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="RD444_000075"
     CDS             71290..73482
                     /locus_tag="RD444_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDT7547486.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /locus_tag="RD444_000076"
     CDS             73508..74470
                     /locus_tag="RD444_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDT7547487.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /locus_tag="RD444_000077"
     CDS             74484..75845
                     /locus_tag="RD444_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDT7547488.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="RD444_000078"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="RD444_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDT7547489.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="RD444_000079"
     CDS             76974..78194
                     /locus_tag="RD444_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDT7547490.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="RD444_000080"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="RD444_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDT7547491.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="RD444_000081"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="RD444_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDT7547492.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="RD444_000082"
     CDS             80956..81633
                     /locus_tag="RD444_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDT7547493.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="RD444_000083"
     CDS             81647..82246
                     /locus_tag="RD444_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDT7547494.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82261..82548
                     /locus_tag="RD444_000084"
     CDS             82261..82548
                     /locus_tag="RD444_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDT7547495.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="RD444_000085"
     CDS             82597..83379
                     /locus_tag="RD444_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDT7547496.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="RD444_000086"
     CDS             83400..84107
                     /locus_tag="RD444_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDT7547497.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="RD444_000087"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="RD444_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDT7547498.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /locus_tag="RD444_000088"
     CDS             complement(87216..88742)
                     /locus_tag="RD444_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDT7547499.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="RD444_000089"
     CDS             complement(88757..89404)
                     /locus_tag="RD444_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDT7547500.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="RD444_000090"
     CDS             complement(89588..89917)
                     /locus_tag="RD444_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDT7547501.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="RD444_000091"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="RD444_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDT7547502.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="RD444_000092"
     CDS             90838..91851
                     /locus_tag="RD444_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7547503.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="RD444_000093"
     CDS             91851..93128
                     /locus_tag="RD444_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7547504.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="RD444_000094"
     CDS             93191..95893
                     /locus_tag="RD444_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7547505.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="RD444_000095"
     CDS             96045..96362
                     /locus_tag="RD444_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7547506.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="RD444_000096"
     CDS             96392..96712
                     /locus_tag="RD444_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7547507.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="RD444_000097"
     CDS             96820..98280
                     /locus_tag="RD444_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7547508.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="RD444_000098"
     CDS             98348..98569
                     /locus_tag="RD444_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547509.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98782
                     /locus_tag="RD444_000099"
     CDS             98600..98782
                     /locus_tag="RD444_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDT7547510.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98782..99195
                     /locus_tag="RD444_000100"
     CDS             98782..99195
                     /locus_tag="RD444_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDT7547511.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99318..100499
                     /locus_tag="RD444_000101"
     CDS             99318..100499
                     /locus_tag="RD444_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7547512.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100570..100734)
                     /locus_tag="RD444_000102"
     CDS             complement(100570..100734)
                     /locus_tag="RD444_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547513.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100888..100961
                     /locus_tag="RD444_000103"
     tRNA            100888..100961
                     /locus_tag="RD444_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100922..100924,aa:Arg,seq:cct)
     gene            complement(101030..102169)
                     /locus_tag="RD444_000104"
     CDS             complement(101030..102169)
                     /locus_tag="RD444_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7547514.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGLEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102468..103103)
                     /locus_tag="RD444_000105"
     CDS             complement(102468..103103)
                     /locus_tag="RD444_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547515.1"
                     /translation="MDNRNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103216..103851)
                     /locus_tag="RD444_000106"
     CDS             complement(103216..103851)
                     /locus_tag="RD444_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDT7547516.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQIEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103885..104346)
                     /locus_tag="RD444_000107"
     CDS             complement(103885..104346)
                     /locus_tag="RD444_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDT7547517.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104476..104907)
                     /locus_tag="RD444_000108"
     CDS             complement(104476..104907)
                     /locus_tag="RD444_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDT7547518.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104925..105245)
                     /locus_tag="RD444_000109"
     CDS             complement(104925..105245)
                     /locus_tag="RD444_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7547519.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105544..106320
                     /locus_tag="RD444_000110"
     CDS             105544..106320
                     /locus_tag="RD444_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDT7547520.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106335..106538
                     /locus_tag="RD444_000111"
     CDS             106335..106538
                     /locus_tag="RD444_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7547521.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106554..106892
                     /locus_tag="RD444_000112"
     CDS             106554..106892
                     /locus_tag="RD444_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDT7547522.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            106879..107058
                     /locus_tag="RD444_000113"
     CDS             106879..107058
                     /locus_tag="RD444_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547523.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(107101..107571)
                     /locus_tag="RD444_000114"
     CDS             complement(107101..107571)
                     /locus_tag="RD444_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547524.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            107658..108356
                     /locus_tag="RD444_000115"
     CDS             107658..108356
                     /locus_tag="RD444_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDT7547525.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            108534..108875
                     /locus_tag="RD444_000116"
     CDS             108534..108875
                     /locus_tag="RD444_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547526.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            108868..109539
                     /locus_tag="RD444_000117"
     CDS             108868..109539
                     /locus_tag="RD444_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDT7547527.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            109545..110231
                     /locus_tag="RD444_000118"
     CDS             109545..110231
                     /locus_tag="RD444_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547528.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            110234..110983
                     /locus_tag="RD444_000119"
     CDS             110234..110983
                     /locus_tag="RD444_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7547529.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            110995..111264
                     /locus_tag="RD444_000120"
     CDS             110995..111264
                     /locus_tag="RD444_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547530.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            111269..111433
                     /locus_tag="RD444_000121"
     CDS             111269..111433
                     /locus_tag="RD444_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547531.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            111426..111728
                     /locus_tag="RD444_000122"
     CDS             111426..111728
                     /locus_tag="RD444_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547532.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            111725..111886
                     /locus_tag="RD444_000123"
     CDS             111725..111886
                     /locus_tag="RD444_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7547533.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            111883..112188
                     /locus_tag="RD444_000124"
     CDS             111883..112188
                     /locus_tag="RD444_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547534.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            112188..112544
                     /locus_tag="RD444_000125"
     CDS             112188..112544
                     /locus_tag="RD444_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDT7547535.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            112531..112749
                     /locus_tag="RD444_000126"
     CDS             112531..112749
                     /locus_tag="RD444_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547536.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            112746..113165
                     /locus_tag="RD444_000127"
     CDS             112746..113165
                     /locus_tag="RD444_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDT7547537.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            113162..113719
                     /locus_tag="RD444_000128"
     CDS             113162..113719
                     /locus_tag="RD444_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDT7547538.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            113716..114012
                     /locus_tag="RD444_000129"
     CDS             113716..114012
                     /locus_tag="RD444_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547539.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            114089..114502
                     /locus_tag="RD444_000130"
     CDS             114089..114502
                     /locus_tag="RD444_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDT7547540.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            114811..114963
                     /locus_tag="RD444_000131"
     CDS             114811..114963
                     /locus_tag="RD444_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547541.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(114960..115235)
                     /locus_tag="RD444_000132"
     CDS             complement(114960..115235)
                     /locus_tag="RD444_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547542.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            115689..115895
                     /locus_tag="RD444_000133"
     CDS             115689..115895
                     /locus_tag="RD444_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547543.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            116091..116258
                     /locus_tag="RD444_000134"
     CDS             116091..116258
                     /locus_tag="RD444_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDT7547544.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            116284..116628
                     /locus_tag="RD444_000135"
     CDS             116284..116628
                     /locus_tag="RD444_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDT7547545.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            116633..116914
                     /locus_tag="RD444_000136"
     CDS             116633..116914
                     /locus_tag="RD444_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547546.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            117017..117331
                     /locus_tag="RD444_000137"
     CDS             117017..117331
                     /locus_tag="RD444_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDT7547547.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            117309..119003
                     /locus_tag="RD444_000138"
     CDS             117309..119003
                     /locus_tag="RD444_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDT7547548.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            119023..120201
                     /locus_tag="RD444_000139"
     CDS             119023..120201
                     /locus_tag="RD444_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7547549.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            120164..120850
                     /locus_tag="RD444_000140"
     CDS             120164..120850
                     /locus_tag="RD444_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDT7547550.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            120850..122010
                     /locus_tag="RD444_000141"
     CDS             120850..122010
                     /locus_tag="RD444_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDT7547551.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            122020..122895
                     /locus_tag="RD444_000142"
     CDS             122020..122895
                     /locus_tag="RD444_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547552.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            122892..123203
                     /locus_tag="RD444_000143"
     CDS             122892..123203
                     /locus_tag="RD444_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547553.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            123193..123546
                     /locus_tag="RD444_000144"
     CDS             123193..123546
                     /locus_tag="RD444_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDT7547554.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            123536..123937
                     /locus_tag="RD444_000145"
     CDS             123536..123937
                     /locus_tag="RD444_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547555.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            123930..124334
                     /locus_tag="RD444_000146"
     CDS             123930..124334
                     /locus_tag="RD444_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547556.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            124346..124954
                     /locus_tag="RD444_000147"
     CDS             124346..124954
                     /locus_tag="RD444_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7547557.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            124974..125336
                     /locus_tag="RD444_000148"
     CDS             124974..125336
                     /locus_tag="RD444_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547558.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            125538..128969
                     /locus_tag="RD444_000149"
     CDS             125538..128969
                     /locus_tag="RD444_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDT7547559.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            129020..129757
                     /locus_tag="RD444_000150"
     CDS             129020..129757
                     /locus_tag="RD444_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7547560.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            129767..132058
                     /locus_tag="RD444_000151"
     CDS             129767..132058
                     /locus_tag="RD444_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7547561.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            132082..134208
                     /locus_tag="RD444_000152"
     CDS             132082..134208
                     /locus_tag="RD444_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547562.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            134225..134374
                     /locus_tag="RD444_000153"
     CDS             134225..134374
                     /locus_tag="RD444_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547563.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            134371..134817
                     /locus_tag="RD444_000154"
     CDS             134371..134817
                     /locus_tag="RD444_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547564.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            134819..134956
                     /locus_tag="RD444_000155"
     CDS             134819..134956
                     /locus_tag="RD444_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDT7547565.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            134994..135287
                     /locus_tag="RD444_000156"
     CDS             134994..135287
                     /locus_tag="RD444_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547566.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            135284..135508
                     /locus_tag="RD444_000157"
     CDS             135284..135508
                     /locus_tag="RD444_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDT7547567.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            135505..136530
                     /locus_tag="RD444_000158"
     CDS             135505..136530
                     /locus_tag="RD444_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7547568.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            136720..136793
                     /locus_tag="RD444_000159"
     tRNA            136720..136793
                     /locus_tag="RD444_000159"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:136754..136756,aa:Ile,seq:cat)
BASE COUNT        45719 a        22901 c        28757 g        40017 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct ccccagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta ttgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaacc
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaagc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggat caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    98761 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    98821 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    98881 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    98941 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    99001 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    99061 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    99121 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    99181 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    99241 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    99301 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    99361 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    99421 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    99481 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    99541 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    99601 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    99661 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    99721 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    99781 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    99841 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    99901 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    99961 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
   100021 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
   100081 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
   100141 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
   100201 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
   100261 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
   100321 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
   100381 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
   100441 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
   100501 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
   100561 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
   100621 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
   100681 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
   100741 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
   100801 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
   100861 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
   100921 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
   100981 ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
   101041 ttttatataa actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc
   101101 gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
   101161 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc
   101221 catgcaaagt aaattgcttt tcaagcccta attcttttaa ttttttgcgc aaatatttgc
   101281 tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt
   101341 ttttgatttc aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat
   101401 caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc
   101461 attcatttaa tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga
   101521 tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtggca ccagcaacta
   101581 acacgagcat aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag
   101641 atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt
   101701 tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca
   101761 acgacgctct aatatggtta ttaaacttaa taacggtaga ttttacatgg tctttagcat
   101821 acttatttaa aaagcgttga tatcccactc tatctaaatc agaaatcaat accgctggca
   101881 tatatttctt tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg
   101941 ttccttcttt ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc
   102001 gatccccttt aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt
   102061 ctccttttgt cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt
   102121 atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcag
   102181 ctctctttct ctgatacaat aggaactata aagaagccca ttgtttgggt tttattattt
   102241 tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt taatggtata
   102301 attggatgtg tattagatca tccaatcaga ggtgtttatt atgattaaag tgatttttaa
   102361 aagaataact gaacttccag agtttagatt atttgttgat tattggtatg tctggttttt
   102421 tattgttgca ttgctgatag gtctagatgt catattagaa agaaaactta agtaagagtt
   102481 gagttaataa attgaattcc taaatgagat attttgtagc ccatattttg tttgccgaca
   102541 tcacctaata ctatggagct aatcgttttt tctaacagac caaaatcaga taatctattt
   102601 aagctaccat ctattagctc ttgtgaatag ccagacatgc tgatatttcc agtgaaattt
   102661 tcgtttccac gactcattaa gcatgctaac aattgaatct caatcgatgt taattgattc
   102721 agtacaagaa gataatactc ctcgttatcc catgaagcgt tgttaattcg aagtaaacaa
   102781 ttcttataaa gatttttata catatctatt ttattttgtg cctttgattt ttctacctcg
   102841 tcatttatcc cttcgaatat tccaatgagt tggtccttgt tctctaagtt gaaaacatct
   102901 tctggaattt gatcgcgaat gttttctaat gattcgttta aatttttgta gaatttctcg
   102961 atgcgtttga aacgcttctc gtttttagac ccaaaataaa gtgactgcac aacgccacca
   103021 atataaggaa ttgctccaat accgccttca actattaatt caagatattc ttttttggtt
   103081 agtttttcat ttctattatc catagttacc tcctattttg tcatttcctt tacaagcatt
   103141 gtgactcctg atggcactct tttgtatagg ttttattatt gtgcacaatc taactcttag
   103201 tggggcgtgg ttcatttatt ttttacttac tttactatta atcatatccc attgagcata
   103261 ggatttttct atgttcgatc ctcttgaact aatttctcct gtaaaagtta ctttatcgcc
   103321 ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa attacatatg aaactttagg
   103381 tgttgcagaa atatcattcc aacttttatc atcgtactct ttgtctgcta taatggctat
   103441 tctagtagtc attgattcga taacagttcc ttcaaaagta tatgttttac cactaatata
   103501 tttagtaaaa gctgtatttt gctcagtagc tggagtaata gaataataac tatctaagaa
   103561 agcaccccag tcatgattat ttgtctctaa aaattcttca atttgagtta ggcttttatt
   103621 gctgctgtta gatgtagtag tttgagtatc agatgatgag ctttcctgtt tgttattgtc
   103681 atttgcctcg tttgatgagg aaagcgagaa acctacggta gtaaaaataa tcccgataat
   103741 aaggagaatc ataggtgttt tcttatttgt ttttttgata aatcttacaa ttaacatgat
   103801 taaacctata ataaataaaa caataccagt aaaaccaaat ataccgtcca ttaatagatc
   103861 ctctttctct tttgatattg aatattataa accaagcaat tcttttttct taaaatcgaa
   103921 ttcttcttga ttaataatcc cttcatctaa caactcttta tactttttaa tttcatctgc
   103981 acttgaaatt tgtgtttctt tggaattatt atctaatata gattgttgct tctctatata
   104041 tttttttaaa ttttccattt cattccaata tttttttgta aacataacgg tgttttcatc
   104101 tttcgtggcc gctaatatac ctccgcgact ttcgtttccg cctagcaagg taaattgtat
   104161 ataaccatta ctcatacctg gtttttttaa ttgtacggct gatatatttt taaaggggat
   104221 agttttttct cctttaatac cttgattaac taaatttata aatccttttc taataattga
   104281 aatagaatca tcagttactt taatcaaggt ttttcctgga gattttatta aaatctcttt
   104341 ttccattttt tcttcctcat ttctgtgata tgattttttt tgtagaagct catatatgag
   104401 gaaagagccc gtgttgcagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa
   104461 taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca
   104521 ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat actccataaa
   104581 tttttcgaga ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa
   104641 aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt
   104701 atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa
   104761 atgaaaggct tctgcagttt caccgtatag attttcattc aaaaaaatga ttctgggttt
   104821 tggatagtag aaaccaggct ctttcatctc catataaact actttcaaat tgtattcgct
   104881 caacatttct ttcaatttca aatacataca aaccatcact ccaactattc attttcctct
   104941 aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca
   105001 aaagaaaaaa cagtatcgtc ttctgataaa tccacatgtt taggggtttc tctttcttct
   105061 ctacctagaa ggtagtctac agagacatcg aaatagtcag caatccgact taattcatct
   105121 gaattaggag tattattttt ccactttgct agataaccgt tagaatatcc aaaattaatt
   105181 tctaactgcc gtatagatat ctttttcctc ttcgccaagt cttttattat ttcataggta
   105241 ttcatcgata aaacaacctt tctaaatgtt tacaaaaaaa gtttagaaaa atacgcagaa
   105301 ataatttgac taattctgag taatgagcta tactatatct tgtaaacaga tttaacaact
   105361 aaaaagacaa caaaaaacac tattgatatg taaatgcaga ccgccaagaa agctttaaaa
   105421 tcaatgtttt tatgtcttat ttaattatgc tcttagtata gagtattact cagatactgt
   105481 caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa aggaatgaga
   105541 aatatgaaca caccgcaaat ttttaatttc gaacaaaacg aagttcggac aattttagta
   105601 aatgatgaac catattttgt aggcaaagac gttgcgagtg ttttgggtta ttcaaacact
   105661 aaagatgctt tgtcgcggca tgtagatttg gaagataaga tggggtcgcg aatcacgacc
   105721 tcaggtcaat caagagagat gacaatcatc aacgaatctg gtttgtacag tttaatctta
   105781 aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacaagtga agtgttgcca
   105841 gcaattagaa aacatggagg ttatctaact ccagaaaaag tagaagaagc tttgcttaat
   105901 ccagatacaa tcattcaatt agcaactcaa ctaaaagaag aaagaactgg aagattaatc
   105961 gcagaacaaa agattgcaga gtacgaacct aaaatctcct atttagatag catattatct
   106021 tctacagatt cagtaacaat tagtcagatt gcagcagatt atgggatgtc tccacaacag
   106081 atgaataaat tacttcataa actaggtatt cagaaaaaag tcggtaacca atggttattg
   106141 tgcaaaaaac acatgaacca aggatacaca aaatctcata caactgagat cccgaaagcc
   106201 gatggtggca ctaaaattgt aatgaatacc aaatggacac agaaagggcg tctatttatc
   106261 tacgaattac taaaaaaaga aggatattac cctcaaatgg atttagagga aattggttag
   106321 aaaggagttt tagtatgact gacattgcag aaatcactca acgagataga gaaaaaatca
   106381 aagaatatgt cgaaagttca aagttcttaa cttacaccat gcttgctgaa agatttggaa
   106441 ttagcaaaag ctacttatct ttaattttaa acggtaaaaa gacttctgca gaagcaaaca
   106501 gaattataga ttcgattatc actatgtacg aattgtaaag ggaggaacag ctaatgcaat
   106561 atctagaagc aaaaatccca attccagaag gctatgtaat tatctcccaa gtggattatg
   106621 aggagttaaa aaaagctgat gatactggta gatggatgac gttgccagaa gtgctagaac
   106681 ggattaacag aaaatatgat tggtttactt ctagagtttt aaagaaccca agatatagaa
   106741 atattatcga tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt gaaggaagag
   106801 acacatatct atttttaaga agtaaaacac ttgaattttt agaaacaaat ttttcggaaa
   106861 tcttaaggag gcaagcggat gggaaaattt aatagagcgc tagtgttcag cgcaccgcta
   106921 atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac
   106981 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
   107041 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
   107101 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
   107161 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
   107221 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
   107281 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
   107341 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
   107401 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
   107461 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
   107521 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
   107581 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
   107641 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
   107701 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
   107761 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
   107821 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
   107881 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
   107941 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
   108001 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
   108061 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
   108121 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
   108181 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
   108241 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
   108301 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
   108361 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
   108421 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
   108481 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
   108541 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
   108601 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
   108661 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
   108721 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
   108781 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
   108841 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
   108901 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
   108961 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
   109021 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
   109081 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
   109141 ggacacacgg aaaccgaatt atttcctgtt ttagattatc gaaaccaacc agttgttaaa
   109201 ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc attggctcta
   109261 cacggactgg gattatatgt atttcaaggg gaagatattc caacaccacc tagaatcgat
   109321 acaaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca gatgggtaaa
   109381 gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt agggctctta
   109441 gctgataacg ttgaaactat tgaacagtta agctatgagc aatgtgcctt gatggagcga
   109501 gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt aaaccattaa
   109561 tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataagc gcaaccatca
   109621 atgaggaagt gaacattgat cgactaaaga cgatgtatga cggctacgat ggtgatcgag
   109681 tcattgaaat tcgttttatt gatcctagac gtttcaccgt acagcaacga aacttcatct
   109741 atgcgctgat aggcgatatt tttatcgata caggcatgcc aacggacttc tggaaggaat
   109801 tcttctactt tcgttttgaa ggtgtcacag ggcgcaaaat aagcctgaaa gacgaatcga
   109861 atacaactgt gagtgatgcc aatgtcttag caaatatcat cttagatttc atctttgaac
   109921 atcatattcc tttcaaagaa ggctatgaga ttttacctgc gaatcaagaa tattacttct
   109981 acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa aacaggagct gacatcgatc
   110041 actttgacaa agcgctagga agacgaaagc gcaaagaagt tgatcatgca gagtacacat
   110101 ttgcagcact ctgcagaatc catcacacag agaagcacaa aataggtgtg attaatttca
   110161 aaaataaata tcaaatcaaa gggatcaaat taaaccagaa aacaatcaaa aagttaaata
   110221 ttggagggta aaaatgacag aacatcgaag ttattatgcg attataccag ccaacgtaag
   110281 gtacgacaaa agacttaaac caaatactaa gttgttatac ggagagataa cggccttgtg
   110341 taatgaaaga ggcttttgtt gggcaggcaa tgagtacttt gcagatttat atggtgtgaa
   110401 taaagagacc atatcgcgat gggtaagtga tttgattaag tttggatact tgaatcggga
   110461 aatcatttac aaagagggta ccaatcaaat aatcaatagg tacctacgaa ttaatcaata
   110521 ccctattgac gaaaaacgca atacccctat tgacgaaaaa gtcaaagata ataatacatc
   110581 tattaataat acatttaata atacaaaaga atatataaga gagttaccgc cttcgaaaaa
   110641 atcgaaggct aagcccgtcc gtcataaata cggagagtat aaaaatgttc ttttgtcaga
   110701 tgagcagatg gagaaactca aaacagaatt ccctaatgat taccaagagc gaatcgaacg
   110761 actgtcagag tattgtgaat catctggtaa gacttataaa aactatttgg caactattcg
   110821 aagttgggca aggaaagaaa aaagtgaacc taagaacgca agcagtggat acaagcgcac
   110881 aggaagacga gagaagcttc ctgaatgggc aatcgaccaa gaagcctatc tcaagaaaaa
   110941 agcgctagaa cgagctaata gacaatcaaa agcaccattc taagaggtgg aaaaatgaag
   111001 atcgattatc tagaactaat taatgaaata gcgaattata aaaagggcga ggaattagac
   111061 gtcctgagag acgtatatga tcaactcgaa gaagctggaa ttgaaggaat taagaatgat
   111121 cgttcgagtt ggagtaagct cagatactat ttcgcacttt atatcgatac aacacaatta
   111181 agaaatttag catataccaa attactattt gttgattgtg ttaaaggctt gcaaaaacat
   111241 cttaatgaac ttgagcaggt gtaatcagat ggacctaaag acatttacag cacagattga
   111301 actaatgcat caagaagctt taagacaaag tgtatcgtac gaagacaagt ggctcaacac
   111361 gttccatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga aaggagaatg
   111421 tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
   111481 ggcaaaatga caaagaagct gcttcagaag tgatgaggct tggaagagcg atgtgggcag
   111541 acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg gcacggtgac aaacttctag
   111601 tgatagggac agctgaacag ttagcaagtc tcacaggctt gcacgagaaa atcgtgagga
   111661 aaagagcaag gtgtggctac acagacgcta agaagagaac gtttagatac gtggaggaat
   111721 catcatgaca acagaagaag tgattcaaat gcgtattcga agccttcagc gtgagattga
   111781 cgatctggaa cgaacaaagg cagtgatggt caatgaaacg gcgagaaagg caatcgattt
   111841 gcacatagag aatttaagaa gggaaatcca tcgattggag gaatgagcgt ggataagaaa
   111901 gcagcaatga aaagaattgc tgaattaacc aagtcagaat cttggcaaga agacaaagaa
   111961 atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga
   112021 acgccgagaa aaattgcaat ctggcatgat gaccgaattt tagtaacagg tactgctgaa
   112081 cagttatctg aaattactgg attaagcaaa aacattatct gggatagagc taggagctta
   112141 tggattgatt caaaaggacg acagtttagg tatgtggagg agagataatg gatctcatta
   112201 cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa gattcagaaa gataaaaaat
   112261 caaaagaacg agctgaatta gttaagttag aaatggctga aacaggagca ggagtgcgaa
   112321 gtagcaggca ttggaaagca gcagcaaaca ttgaatttta ttacaacgaa attcaaaaag
   112381 ggttcgatca gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa cttcatcaag
   112441 atcgtttcaa atttgtagag aagtataaag aaatattaga agagtatttg aggaggacag
   112501 caaatgataa aaaaactcgt tcaattcagc atggatttat atgatatcga atcaggagca
   112561 acactatctg tggaatcgga ccatctaatc ataaattttg gtggaaagcg ccagattatt
   112621 ttgtgggtag ttgatgatgt actgtttcca gaaattgttc atgatttcga agaatcaaaa
   112681 gcggttgagt ttgaaatagt gaaaaaagta atggaattga ttgaaaaata cgaggaggac
   112741 agcgaatgat accgaagttt agagcgtggg ataaacgaaa gaacgtaatg agagatgtag
   112801 ccgtcttgca ttttactaaa aacggcaaaa caaactttat tgaatattgg ataaatccta
   112861 ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat gcaatccaca ggaatgaaag
   112921 ataagaatgg tgtggatata tttgaagggg atatagtatt agtcagcgtg cgaaatggct
   112981 tcgattactt agataataaa gtctgtattg tcaaaaattc aatagattat tccggattag
   113041 tttgtgccac tgttgatgaa gacttagagt atcaaatttt taacacagag ctgtttgaag
   113101 aatacacgta cgaagtcacc ggaaatatat acgagaatag cgagttattg gaggaacagc
   113161 gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt aaagcgatag gaaatgatgc
   113221 aatagctgct tgctataacg agagtataaa ttcaggtatt acgttaatga aaaaaataga
   113281 cgaaccgcag aaaccagtta ttccacaact cgtggccggt tggcttgaga aatctacgga
   113341 cccttttaca aaagctgaaa aaatagcgta tttaatcaaa tctaaagatg gtgattcata
   113401 ttatttctgt gattggtttg tacgagatgg catagtgacg caagagcaag gagaagaatt
   113461 actcgcttgg gcaacaagac aatcatatga aacactattg agcctataca acggctacga
   113521 ggttgagaaa gagccgttat gggcaataaa gaatgccgat ggaaactatc ttactaaatg
   113581 tgctttatgg ggaaaagatg gagtaaatta tagttttgaa tgcaatccat ctcatcgatt
   113641 gcttttcact gataaagcaa cagcggatgc tgcagcattg ttggtgaatg gaacagtgga
   113701 agaggtggta gaaagatgaa actaaaagac ggattttacg ctagcagtca cggtatcggc
   113761 ggtttaatgc tagatatgcc gacaaagaac cctaaaacac gcaagaaatc aaaattcaaa
   113821 gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt atccgttcag aggatacgtt
   113881 aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa atacaatgaa gtgtgatcgt
   113941 tcgactgcaa aacacaagca ttatttagct gtagcgagat tagttgacat ggaagtaatc
   114001 aagagcaaat aaaaaaagcc ggatcgctcc gactgattca ataaatccaa cacatttatt
   114061 atatcacata aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca
   114121 gacaagatgt aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg
   114181 tcgctctttg atagatatta agtcgccgat tataacggat atgccgaagg caccgaagca
   114241 tggcaataag gcagaagacg cgattattca gatgatggat atagaaacgg agagagatgc
   114301 gattctagcg gctttgatgg ctcttagtct gattagtcgt cagatactct actacagctt
   114361 ctgtgtgcca gatagcttct caaactacag aattagccgt gaagtgggtt attcagaaag
   114421 aagtatacaa cggatgaagt cggaagctct aatagagttt gcagaagcat ataaacacgg
   114481 aaaaataatt gcttataaat aatttggcgg ttttttggcg gaatgatggc ggtttttaga
   114541 catttaccag tgttattatg gtagtgtcga aagattagga aacaggatcg acaaaataaa
   114601 cgaagggagg caatctccct catcgtttta aacttcttct tagatagaca gcaactaatt
   114661 atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt catcggttgc tgtttatttt
   114721 gattagacag cagcactcac aaaactaaac atgttcttgt cgtctttcaa ctgctgctgt
   114781 ctattaattt atgtattgga gggaagaaaa atgaaattat cagtagaagg cactccagaa
   114841 gaaatacaag aattgctcca agctattggt agtagcaagg agcaaaaggt aagtgtttct
   114901 aattcagata ttgacggtat ttgcaattgt ttaaacagag aaaaaatatc taatccttac
   114961 taagagaaaa tatgttcgtt aatgaatttc caattttcct ctgtagctct accttgataa
   115021 tttttttgca gttcacagat gaataaggaa tcgttattat caagtactgt atttaatttt
   115081 tcacacattt gatttggatt caaattagtt ttaactaacc aaaaagattt ttgtagcttt
   115141 atatatgccc caaattcttt tattatttca aatactttat catacttttg ccctgggtta
   115201 ttaagatcat aactaatcat gtagggttta tacatgttgt cttgcacatc cttgttttat
   115261 atttcagcgg accactcgct gataaataaa attatacgct tagtatttat tttcacaata
   115321 ttaatttatc gtaataactt ttgagtaaat agaacaaaaa acctgcacta gtttccgcta
   115381 gtacaggcag tgactatatc ggttgataat ctagcatata ttaaaaataa ttgcaagaaa
   115441 agaaatttat agtggtttat accaaattat caaagcaatg ttattttgtt gctgtctatt
   115501 gttttttaat tattcacacg ataactaaag gtgggtgaag agaaatgatt ccattaataa
   115561 tttcaatttt tgcgctctgt cttaatgtct atatgattgg atttaaaaat gggcaaaata
   115621 aaaaatagta gcagccaaga ataattttat agtgtcactg tggcggaaag ggtagacgct
   115681 aagcatgtgt gctaggtcaa tgcttcggca accatgcaag gttcgattcc ttgccagcga
   115741 cattaaatgc ctatgacggt tacgactacc gaaaaaagat cgttaagaag ctatacggtg
   115801 ctacgtacgg caatgtagta agtgtgctat ctgtacacca ccaagcttcg gtcactgtgg
   115861 cggaagtaga agacgcagcg gtaaatggcg agtagcctcg tgagagcctg gtaagttctc
   115921 gtgagtggtg caatcccact ccagcgactt taagcaaccg aggcatcggc ggtttaaaaa
   115981 tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg caagttacta ttacatatta
   116041 gatcactctt tgagtggtct ttttattttt gcacaaagga ggaaacaaca atgtatagac
   116101 cacaatactt agaacagaag tatgaagtaa tcactgttca taaaggtaat ggcgaaaaag
   116161 tatatgagta tagaagacca ataaagagcg atacatataa acgaaaggaa aacaatgaag
   116221 ttattccatt gtatggcaaa agaatagcta agcattaaat aagattgcga aaggagacgg
   116281 aacatgaccg aggaattcta tagatggcta ttacagttga caagagaaga tcgtttggtt
   116341 aagttctatc agtctcctaa atggcgcagg cttagagaga aagcgatgaa acgagatcac
   116401 tatgaatgcc aagagtgtag aagactaggt aagtatcata gagtagagaa cgttcatcat
   116461 ataaaggaag tcaaggatag acctgactta gctttagatt tagataatct tatttgttta
   116521 tgtgttgaac atcataatga agttcatggc agatatctta cagcattaga taaacaagag
   116581 aagaagatag aaagctttgc taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat
   116641 caatgataat ggcagagagt atgatacaga aaagattgaa gagtattcat cttatactca
   116701 gggattaatt aaacgtttga tatacgttcg ctatgtaggt attagggatc tgttatcaga
   116761 taactgttgt agtaaataca aagtgaatca agtaagagaa gcgttgaata aagataataa
   116821 cgtcgaaaga ataaaaaatg tttttggata tagtattgaa gagattaatt attacattga
   116881 cttcgctgaa gctttcattc cgatggtgag ataacccccc cttaaaataa atcgcaaatt
   116941 ttttgggggt gatgaaacgg agggggctgt caggaaaaga gattttttcg aactttatca
   117001 tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt
   117061 acgccgctct ttaataagcc aattggaaag tcagaaaaca aatattgagc cattcttaga
   117121 taatgttgat cgttatatca gtttatggga aacggcgata tcactggaag aagatatatc
   117181 cgagaacggc attagactgg agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc
   117241 tgtcaacaaa caaatgggat tgatgttgga taaacttgcc attactcctg aattggtagg
   117301 tgaagcaaat gaatcaattc ctgagttata agcatattga aaattggttc aaagctatag
   117361 aagaaggcac tatcaaggta tgcaaagagc aattattgct aaaaaattat ctagaagaaa
   117421 gagtctttac tagagaagat atttacttcg ataagcagat ggtagaggat tcaatcaata
   117481 taccagcaca atactttcca ttcgaattaa ttccgtggga aaaatttcta caatgtttta
   117541 tttatggtgt tcgatggaaa aaagataaaa cactagtgtt caatagatat ctttcattaa
   117601 tgggacgtgg taatggtaaa actggttttg cttcttggaa caacttcttt ctactaaccg
   117661 ctaaacacgg tattaaaaat tatgatattg atatctatgc caataatgaa agccaagcaa
   117721 agactagttt tgatgatgta tttaaagtaa ttaaagatca tcctgattta gataaaaaag
   117781 tatttaaagc tacgaaggaa gttattcaaa atatcgctac aaacagcaaa cttcgttata
   117841 acacggcaaa tgctagaaca aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa
   117901 ttcacgaaaa tgaagattat tcaatgataa atgtggctac ttctggtggt ggtaaaattc
   117961 gagattatag agaattttat gatacaacta atggtcatgt tcgtggtggt ccgcttgatg
   118021 acattataga agaatcaaaa atgattcttt ctggagaact tggaattgac aaggatggag
   118081 cagaattttc tagtttgttt ccatttattt gtcgcttgga taacgataat gaagttgatg
   118141 atcccgacat gtgggaaaaa gcttgtccaa ctattaatta caatgcagat ttaaaacgga
   118201 aaatgtttca agaatactct caaatgcaac gtaatgctgg tttaagactt acgttcatga
   118261 ccaaacgaat gaacagacct atggaagata cacgatttgc tgttgcttca tatgatgatg
   118321 ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg
   118381 attttgctga tagacgagat tttgccagcg ttgggttgct aggaaaatac gataaagatg
   118441 tttattttac acaacatact tttatccacg aatcagccct tcgattacaa aacatcaaac
   118501 gagaggttat agatatttct atagatcaag gaaaatcaca gatcgttcat ggaaaaaata
   118561 tagaagctga ttatattgta ggttggtttc ttgaaatgag taataaatat tatattaaaa
   118621 aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt
   118681 ttactgtgga aattgttcga agcggatctg ttacacatgg tatgttaaaa gatctggttg
   118741 atgacctttt tattaatcaa cgtttatttt ttggtgacga tgcgattatg cgttggtatt
   118801 gcatgaatgt atatgaagag catatttcta atggaaatat acgctatgaa aaaatagaac
   118861 ctgaaactag aaaaacggat ggcttttttt cattccttca tggtttgaat tttttagatg
   118921 atatttatga ttctgctcct gtaacagtca caaatagctc agtagaaaat acaggaactg
   118981 gatttactcc tctagtattc taacttgaaa ggaggtgaga aagtggggat ttttcaaaag
   119041 gcggtaggat acttcacaaa aaaagcaacg gttcctttag aagaatactt ttgtaaattg
   119101 caagttgatt ttgtgtatcg aaaatttgcg attgaaactt gtattgattt gattgcaaat
   119161 gcgatgagta aagcggaatt caagtcatat gaagatggaa aaaataaaaa gaatgatctt
   119221 tactataggc tgaatgtagc tcctaataag aaaaataatg caacagaatt tagaaaaaaa
   119281 ctgatcagga gattaatatt ctacaatgaa gtattgatcg tttctccgtc taataattct
   119341 agcgaaatat ttattgcgga tagttgggat gtcacagaat atgcattgaa agatgatgtg
   119401 ttttctcaag tgcaaattaa caacatagtc cttgatagag aatttctaga aagtgatgtt
   119461 atctatataa aatacgcaga tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg
   119521 tatgggaaac tcatttctag tgccatgaat gtttacaagc gctctaacgc tcgtagatac
   119581 gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa cacaagatca aatcaataaa
   119641 atgatgacat cacaatttaa ggcttttatg gaagctgata atgcaggtgc ggtatttcaa
   119701 ttacaaaatg agtacacatt agaagatttc agcggaaact tccaaagcaa ttcaagagat
   119761 ataaaaaact taatagacga catctttgag atgacagcag cagcgtttca cgttccgaaa
   119821 aacctactaa agggagacat gagtgggtta tcggatcaag tggacgcttt tttaatgttc
   119881 gaaatcatac cgattgctga acttattcag gatgcgttta acgctagtct ctatgaagta
   119941 gaagaatact tgtcagggaa ttttgtacgt gtggatacaa ctatgatcaa gattactagc
   120001 ttcaaagatt tggttgacgc tattgatgta ggcattagaa atggagtatt tacaatcaac
   120061 gaaggaagag aacgcgttgg aaatgatcgc tctgataagg cgatggcaga tgaaatattt
   120121 ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac
   120181 atttctagca gtaaagaatg aaggcgcagt accgcaaatt tttattcagg gatttattgg
   120241 ttctagttgg ttctttgaag ggaatactga caagggaatc aaaaatattt tggatagtct
   120301 aggtgatcaa gaagaaattg aagtagtaat taattcaaac ggtggagacg tatttcaagg
   120361 gattgctatt gggaacttac ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg
   120421 cttagccgct agtgctgctt caattatcgc aatggctggc gatactataa aaatctacaa
   120481 caatgcacaa ttgatgattc accgcgcttc cacatacgga gaaggaaatg tcgatgactt
   120541 ccgtacgatt gctgaccaac tggaatcaat tgataaatcg gtaaaggctt catataaaac
   120601 acgattcaat ggcacagatg aagcattgca agaacttctt gaaaaagaat cgtttatgga
   120661 tgcagaaaca gctttgagtt atggattggt cgatgaaatt atcgatgcag aaaatagctc
   120721 aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa
   120781 aagagcagaa aaaattgctg catttacagc agcattaaat aaaacatttg gacaaggaga
   120841 tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg
   120901 aaagctttta aagatggtaa cgaagaatct tttagcgcag ctatggtaag cttatctaag
   120961 gaaattcagg ataaaatttt agaagaagca acagcaaaaa atcaagatca attagtatta
   121021 atgaaccgtg gtcagcgtgt attaactaca caagaaacaa aattctataa cgaagtagtg
   121081 aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta
   121141 tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa ttacttttgt taacacaact
   121201 ggtgtaacag aatggattgt gtcacgtgga gtcaatccag catggtgggg taaactttgc
   121261 gaagctgtta aaaaagtttt agataatggc tttgatgtaa ttaacatgaa gcagttcaag
   121321 ctatcaggtt atattcctgt atgtaaggca atgcttgatt taggtccagt atggttagat
   121381 cgttatgtcc gtactgtttt agtagaatca ttgagaattg cattagaaca agcaattgtt
   121441 gatggtactg gtaaagatat gccagtcgga atgatgcgtg acatgagcaa acaaactagc
   121501 ggagaatatg ctgaaaaaaa agcagaacct attacagctt tagatgctgt aactatgggc
   121561 ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt
   121621 aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa ctgattactg gtctaaagta
   121681 ttcccagcta agactgtact aactgctaat ggagaatacg tacaagtatt gccagtacca
   121741 gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag cagttattgg ggtagcctca
   121801 gattacttca tgggtgtagg atctacacta aaaattgaag cttcagatga ataccatttt
   121861 gttgaagacg aacgcattta tctagctaaa caatatgcaa acggtcaacc taaacgtaac
   121921 gatagtttca ttgtattaga tattagcgct ttgggaacta ctactacaac tacaaaacca
   121981 acaaccacaa caactacaac acaagcgtag gtgatcagaa tgaagtatat tctttgtcag
   122041 ccggcaatca atcggtttaa atgggagctt gaagtttgtt taactaatct gaagaaacta
   122101 ggaatcaaag atatcgtatt gcttttcagc agacacgatg atcagattcc tatttttttt
   122161 gagaaggaat atggtgttga agttcatgtg tacgatgatc tgcgggacga caaagagtat
   122221 attccttcga ttaaaccata tttatggtgg aaatatttag aagaagatca ttcgcgtgag
   122281 gacgaccgat atttctatat cgattcggat gtcattttca ataaaagaat taatttgcgc
   122341 aaattgcctt ctaaagatga tgtttggtat tgtagcgact gctgtagtta tctaagtctt
   122401 gattatatta gaagctgtga aaacggagaa aatattctaa aagatatggc aaacattgta
   122461 aatgttacag tagaatcttt ggaaactata aacactaatt caggaggcgc acagtgggtt
   122521 attaaccgtc ctaaagcgaa ttattggaaa aaggtttatc tggattctaa tcggctatat
   122581 cgctacctta gagggcaaaa aacaaatata caaatctgga cagccgagat gtgggcacag
   122641 ctttggaaca tgatgtattt caatattggt cctaaagttc acgaggaatt agacttttgt
   122701 tttgctactg atccaataga aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta
   122761 acaacaaacg atgaagattt atttttcaaa gggagatacg tgacttctac gccttttgat
   122821 gaagatttat catttgtaaa caagaaaaaa tgctcttacg catatgttaa agcaattaag
   122881 gcggtggtta gatgacgcct gaacaagtga ctgaagaatt gctaatagct gtgaaggata
   122941 atatttacgt tacctggaac gaagaagatg agtcaattaa aaagatgata gctaaaaatg
   123001 ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt ttctcctgaa agcttagaat
   123061 acggattgct aatcgaaaga tgtagatacg actggaatcg tgctttagat gagtttgaac
   123121 aaaatttcgc tagtgagtta ttaggtttca ttcaacatta tgcgctacaa gaatatattg
   123181 caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa cattcaacga tggttggtta
   123241 aagattttga cgcaaactac caaaagaaat gaactaggaa aaaagattgg tgtagaagat
   123301 acagaaatca cttctttaaa atttagaaat ctttccatga gagatagtga tataacagct
   123361 atggatgcga tgggatcgaa attaactaag aaagtaaaga ctccatttca tccaatcgcc
   123421 aagaaattta ataaagatca atattttatc gtaatcgata gtatgcgtta caacgttatc
   123481 tatgccgatt acgataattt ttatatctat ttttatcttg aaagtgtggg tgaatatggt
   123541 gattgataat tctaaagaaa aagaacgttt aaataagcaa atttctgcta tcaaaacttc
   123601 cttagaagag cattttggcc tcaaactctt tcaagactcc gtaggcgagg atgagctacc
   123661 tgatgatttt aattacttca ttctcgaaac aggagaaata gaaatgatca ctgagccaaa
   123721 atatagcgtg ggtcaaaatc tatatctaac tttctattca gaaaatagag aagatttaac
   123781 aggagactca ctagatatta tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat
   123841 ggatcccaat catttaaaac tagagaacca agatcgctat atcgatcaat tggtatttac
   123901 gtttagacga ttattgaaga gtgattgtca tggctaaaaa tagttgggag ctaaaaataa
   123961 atggacatga tgaacttctt gtgcggatgg aacgctattc aagcgagagc gaacgactga
   124021 ttaacgaagc attgaaatca aaaggttcgg ctattgcagt ggataggatt acagaaaaaa
   124081 ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac
   124141 ttaagactca atacattaat ttgggtttca tcattagacc tacaagaaaa tttgagtatt
   124201 taaaatatcc tgatttgggg ataggtactt ctaaaagaaa tcagccggac gaatttatga
   124261 gaagaggatt aggtcttgca cttgatccaa ttacagaact tctgattcgt caattcgata
   124321 aattaaataa atagggggaa caacaatggc taaaacaaaa actgtagtaa caacgttcga
   124381 taacgtaagt atcaaacgaa ttgcttttaa ttttaagaac gcaggaaatg caatcgcaac
   124441 gaattgtaat ggacaattag atggcgaaac agaaatgcaa acggtggtta aaaaatgtgg
   124501 agcgacagaa gtaaaatcaa aatctaaacc aatcaatatg acggtaacaa ttactgcaca
   124561 tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa caagatggac gtattaaacc
   124621 aggcatttac tcttacggtc ctgattccgt aggcgaagat ttctcacttg ctgcagagat
   124681 cgtggatgac ttcgaagaaa atagaaagtt agttggtatg ttagcatgca cttcgaatac
   124741 aggattaaca ttctctattg aaaatggtgc ggatgaagta gctgcgttag aactagaaac
   124801 aaaagttatg caagatgaat ttggtaaatt ttatcatgaa gcaattgttg cagaacttga
   124861 agaagactta acagatcaat ggatgacgaa tctatctgct gatgtgatta aaaaaacttc
   124921 agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg
   124981 aagattactc aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa cctaaactga
   125041 atatcaagaa attattgatg atcaatagag attttaacac agacgagttt gcaaaaatga
   125101 ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct gtgtatattg
   125161 cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc gataaatggg
   125221 attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa caagcacgcg
   125281 atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
   125341 tccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
   125401 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
   125461 caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatccc agaaaaacta
   125521 aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc agaagtaact ttcatagcta
   125581 acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact aaaaatagag
   125641 cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca gacaagttag
   125701 gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagtcaaaaa gttgaagtaa
   125761 ctagccaacg tttagccaat gcgaaaaaat attatggaga aaattccacc gaagttcaga
   125821 aacttgaaag agaactgatt aatcaacaaa cagcgcaaca acgtttgtca aacgaaattg
   125881 ataaaacgag taatgcacta gctcaagcaa aaggcgaaat acagacgtac gagtctacaa
   125941 tgcaacagtt ggatagcgaa caaaaaaatg ttcaagctag tgcttctctg attgaatccg
   126001 aatacaaaaa atggcaagca actgctggtc aatcagcttc tgaagccgag aaattagcga
   126061 aagcccaaga atatgtttct caacaatctg aaaatgcgga gaaaacgata gatatcctaa
   126121 gacgacagtt agaagctaca cagtctgaat ttggcgctac atccacagaa gcaatgcaga
   126181 tggaagcgaa gcttaatgat gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa
   126241 atgtagatac aactaacttg gacgatatcg gaagcaaaat agatatgaat aatttaatgg
   126301 aagcttctga cgttttaagc gacattggcg ataagcttac agaattaggg aaacaagcag
   126361 tggactctgc taacagtgta ggtagttccc agagtaagat acaagctaat tttggtttgt
   126421 ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga catttattat aaaggttttg
   126481 gagaatcgtt agatcagtcc acagatgcat tgattttggt aaagcgtaat ttaggagatt
   126541 taaataatca agatttacaa aatatcacgg aacaagctat ggtcctagaa aacaccatgg
   126601 gcgctgatat ggatgaaacg ttacgtggtg taaatggctt aatggtcaat ttcggcttga
   126661 gtgctcaaga tgcaatggat ttaatggttt cgggtactca aaacggttta gataaaacgc
   126721 acgaattagg cgacaatatg gcagaatata gccaattatg gagtcaaatg ggatattcag
   126781 ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc gggtgcttat aaccttgata
   126841 aagtcaatga cttagttaag gaaatgggaa tatcgttaac agatggtcga tttgagcaaa
   126901 acatggatat gtttagtgaa agtactagaa aagcttttga agagtggaaa aatggcggag
   126961 gaacacaaaa agacgttatt aattccatga ttcaagattt tagcaatatg gatggtcaat
   127021 acgaccaatt aaataaagct tcgacaattt ggtctgcgct tggcgaagat aatgcgatga
   127081 aagttgtcca atctttaact gatgttaacc atacatttga tgatgttagt ggatctgcac
   127141 aaaaaatgaa tgaagattct actactccgt tgcaagagtt gaacgggaaa atagctgaat
   127201 taaaggattc attagctcct ataggcaaca caatcataga tgcactcgaa ccagtaattg
   127261 attttctagg aaagatggct gatgcgttta ataatcttcc acaaccagta caggatttca
   127321 ttgttgctat aggtggtctt acagcagcat ttggcatact gttaccggca atactagccg
   127381 tgtcatattt atttggtccg atgatgctta taattggagg aattatagct gttatagcag
   127441 gcgttattgt ggcaattaag aactggggtg caattactga ctggtttagt ggtttatgga
   127501 aaaaatttac tgattggttg ggtgatactt gggaaagtat aaaagacgga gcctcatcag
   127561 tttgggatgg ggttaaagaa acctggtctg gatttgtaga ttgggttcaa gatatttggc
   127621 aaggagtttc tgattggttt ggagagctat ggagcggatt agttgaagga gcttccaaca
   127681 tctggcaagg agtccaagaa acttggcaaa cattcgttga ttgggtttca aatatttgga
   127741 acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt gttcaaatac
   127801 catggacatt aataacgtca ttgattcaag ctggtattaa tattatcgtg ggtatttttg
   127861 atgtagctgg acagttattg ggcgcagctt ggcaagctgt ttggacacct atttctgatt
   127921 tccttaaaaa tacttgggat actatgacac aatggataag tatcgcttgg aatgggattg
   127981 taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt atatggacag
   128041 ctattagtac tacggcttca aatatttgga attcaattag tgcaacagct tctagtattt
   128101 ggaacagtat caagaataca atcactagct tggtacaagc agctgctaca gtaattcaaa
   128161 atatttggtc aactgtatct agttggttag gtggaatttg gaattcaatc agctctacag
   128221 catcaaatat ctggaatagt gtgactagta gtataagcaa tgctataaac gcagctaaaa
   128281 gtgccattca aagtgtttgg aatagtatat cttcgtggat tagcggaatt tggaacggta
   128341 tcaaaaatac tgctttgaat ctttggaatg gaattacaag cactattagc tctaaagtaa
   128401 acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct aacaggtatt gtttccgaca
   128461 tattcaataa tgttaaaagt acaattgcta acatatggga aggcatcaaa aagactgtta
   128521 gcgctccaat tgattggatt agagacaaaa tcagtggcat ttttgataat ttgaatattt
   128581 ctataccaca tattccgtta ccggaattta tcatggaagg cagctttaac ccgctaaaag
   128641 gtcaaatccc ccatttgcgt gtaaaatggc atgctaaagg aggtatcttt actaaaccaa
   128701 ctttactagg tggaatgaac ggtgtcggtg aagcaggacc tgaagcagtt ttacctttga
   128761 aaagatctgt tttgcaagaa attggtgatc gtatcttgag tagcacatca gtttcatcta
   128821 gggcacaaac gattcaacct gtgaataact acgaattcaa tttcacaatt gatggtaacg
   128881 cagatgaggt tactatgaag caaacaactc aacaaatcat tgatagcatt acaaaaattc
   128941 aaaatgataa tgcttcggca tggcgttaaa caggagagta tttctcctgt ttttttagta
   129001 ttaaaaagga tgtgaaaaaa tgactgattg tatacattct ataatcgatg gatttcctga
   129061 ttatttgcat aaattggctt tagctgaaag accaaccata ccttctccaa aaagacagag
   129121 agttgaaact tctgttttag gtaggttagg tggcttagta caagattact cgtttgaaga
   129181 tatgtcgttt acattgcact ataactattt agaggatgtg gaagaccatc aagcgttcaa
   129241 gcaatcgttt tatatcatgc gtcattggtt aaactatgca aagaaattag aattctctga
   129301 cgatcccaac gtctattacg ttatccagac tatcgatatt ggggatgcag aaaacgatat
   129361 tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga
   129421 agatgtacct ataaccgtag ataaaccaca gtcatttaac ttgctgaata atagtttaga
   129481 agaaagtttt ccaaagatta tcatcactcc ttcagctact tcatgccagt tcatcttaaa
   129541 tgattatgtg tttagtttcg aaggcttagt agcaggaact gatgtagtca ttgatagtga
   129601 tttgatgctt tgctacgaag agcaatcgga cggagatatt ttagatcggt ccaacaaaat
   129661 gaagaccatg caatatccga cattgcaagt ggatatcaat cattttaatt gcactggttt
   129721 gagcaaaata caaatttatc gtaacgggtt aaggtaggtg aaatagatga tcgataattt
   129781 aataactatt tacgataaaa atgacgcgaa taatttagct gaacatttat atgatacgca
   129841 aggtttaggc gctttgtcag actggttaac agctactgtt agcaataaac taaacggagc
   129901 cgagatattt cagggtactt attcaataaa cggaactaat gcagatttga ttgtagacgg
   129961 acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta
   130021 tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa gctgaaccta ttttcaatga
   130081 tataagaaaa tcggtgttga ataaatatga cagcggaaca gaaaagatca ctgctactca
   130141 ggcatggcaa aacgcaaaag ttttagcgaa accagctatc ccttcgcagt tttctttctc
   130201 gtcattagta gatacgcttg ctaatgtgaa gatagaaaag gcgaattttt tagaattctt
   130261 tggtggaaaa gagggatcta ttctagatcg atttcatggt gagtttctaa aagataataa
   130321 cacattacgt catgaaaaaa gtctaggtac ggatcataaa atcaaagcga tttatactaa
   130381 aaacttaact ggtcttgact tagagattga tgctcaaagt gttttagttg gagtttatcc
   130441 attcattagc agctcttcag aaggagaaga cgagatcact ctaccagaag aagttatttt
   130501 cacggattac gtggatgatt atcctgctgg atatgtttct tttgttgatt ttaaagacaa
   130561 agcgactgat gtagccacat taagggaagc tgctaaagac tggttgaaaa caaacataga
   130621 taaacaaaaa ccacaagtga gtggttcgat tgaattagta ccattgaggc atcaaagagg
   130681 ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt gacggagtag atgtgtatca
   130741 tccacagtta aaagtgaata tgtcagcgag aattgtggaa tatacgttta atgttctaac
   130801 caattcatac gataaattag ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga
   130861 gaataatgtc agcaatttga ttaatgatgc cattgatcaa ttgaaaaacg gtggcgaaat
   130921 cagtgattta ctcaatgata ttgtagatca tcaaactgat atgattactg gtcaaaatgg
   130981 tggttatgtt ttattagatc ctaaagaagc gcctagtcgt attttgatta tggacacacc
   131041 agataagaat accgcaagga atgttttaca aatcaacaat gctggtattg gtttttctaa
   131101 aactggcatt aatggaacat atgaaacggc atggacgtta gatggcggat tcaatgcctc
   131161 gtttattacg gctggtgaaa tagtaggaat tactattaga ggtactacat taattagtga
   131221 tggtgctgat tatagaacaa gtattgctaa tggcaaaatg acttggtact caaaaaaagt
   131281 taacaaagat attatggagc tagaagcacg tgattatgta agtgctgatg ccggtattgt
   131341 atcatacacc atgaaaactg gtggtggttt catgattaga aatccacagg gtaacttggt
   131401 ttttagtacg tgggataatg gtaataacag accgtttcta tcttttggtg cgcccaattt
   131461 caggtatagc aatgctagtt atgtaacttc tggcgacggt agttctttaa gcattaatgg
   131521 tagtgcgggt aactcatggg aatttaaggt agctggtagg actatgaaat ttactagtga
   131581 tggtatgcta acgttaccag gttgtttttt tggttcatgg gaagatggga aacttgctag
   131641 gtttgaacaa tcaacggtac aagtatataa agattttact gttagaggta ctaaaaactc
   131701 aactgtacca acagaacatt atggacaacg actattgaac gcttatgaaa ctccagaata
   131761 ttatttcgct gattatgggg aagccgttac aggtgacaat ggtaaagttc gtgttgatat
   131821 tgaccccatg tttgctgaaa cagtaaatct aagtcggtat atgacacatg tgacacctac
   131881 agaactagtt ttgtgtgctg ttactcatga agatattgac catttcatca ttgaaactag
   131941 taagccaaac gtattagtta gatggaattt agtggcacac cgtctagggt atgaagatat
   132001 tagattaaaa gaggatacag catatgatag cacagtgctt gaccaaaaac gtttttaaaa
   132061 cgaagacaag gaggtatata aatggctagc agtttatata atttggcttt agatttcagc
   132121 aaagaattaa actacaccaa agctattatg gctcgtcaag gtgataaagg gattacggtg
   132181 acggttaaac cgtttctaaa tggcttgcag atggatacga gtggcggaac atttacttta
   132241 aaaggaacaa caccatctaa ccgttacgta gataatgttg caactagtgt aactagtgaa
   132301 gaagtcacgt tttctcttga tggcacattt atgagtgaag caggatatta taaacactgc
   132361 tacgtagaat atagaaaaga caatcaaatt ttaacaacgc aagatatcat ttttttctca
   132421 ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat atgtttcgca attagaagag
   132481 ttgattcgaa agtacaacga aacttttgat gcttttatgg ctgaaatcaa aggtagagtg
   132541 gatagcttaa atcaacagat tactgattta actggtcaag ctaaaacgct acaagacaag
   132601 ttagatgctc tgaaagaaga aatttctaag ttaggtaact tacaagtgat gtacagtaac
   132661 agcatcgact tcggggacta tgattattcg gagaacccga atttaatgcc atatattaca
   132721 gaaccttggg ttggaccttt gttaggtaat ggacatacag ttaaagatag tgttaaaagg
   132781 gttataactc atactaaaac tagaacagct aattccggcg acatattatc gcttggctta
   132841 ggtattccgt gtacagcgga agctaataat aggtatctta taaccacttt acgaccatct
   132901 acgacataca cattaagcgt tactatgagt gtcggttcag attggactgg agagaccaat
   132961 actatagggg ttaggcttag atatttaaat gaacaaggtg gtatagaact gcccattaat
   133021 gcattaatac cagcaaatgt agaacgcgat aaaatggtca ctcatacatt tactggtata
   133081 acaaaagata atgtaactag cataacaaac tgttatgttg aaatattctc acttaatagt
   133141 gaatataaag gaacggttag tgtcagctat gacgttaagt taaaagcaca ctaccctaac
   133201 ttactagatg gcccttactg gttaggtaaa gttcctttag gcgagaatat tgctgaccct
   133261 acagttgtct ttccacacaa aactagtgaa tatatggttt atggtagacg caatacagaa
   133321 aactatatcg ctgaccaaac atataccata tcaatgaaag caactaaact aacagttcaa
   133381 tcgtttgccg tgtatattgc tgctggccgc gttaaagtag gagacatgaa gcctactgag
   133441 gggttagcta atacatggga gttaactttt actgtaacta agcaacatat agatagtggg
   133501 gtaactaatt atctggaaat ataccaatac ccgtcagcta caaaaggagc tgttcaaatt
   133561 gagtggctca aactagaaaa aggcaatact cgaaccccga atattagtga gtataaatat
   133621 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
   133681 gaatatgtcg aagataattt ggccacagat attaaaattt ctgaaattac tggtaaagca
   133741 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
   133801 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
   133861 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
   133921 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
   133981 tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg attcaacgat actaacaatt
   134041 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
   134101 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
   134161 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
   134221 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
   134281 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
   134341 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
   134401 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
   134461 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
   134521 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
   134581 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
   134641 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
   134701 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
   134761 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
   134821 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
   134881 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
   134941 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
   135001 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
   135061 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
   135121 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
   135181 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
   135241 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
   135301 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
   135361 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
   135421 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
   135481 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
   135541 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
   135601 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
   135661 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
   135721 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
   135781 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
   135841 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
   135901 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
   135961 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
   136021 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
   136081 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
   136141 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
   136201 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
   136261 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
   136321 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
   136381 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
   136441 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
   136501 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
   136561 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
   136621 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
   136681 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
   136741 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
   136801 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
   136861 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
   136921 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
   136981 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
   137041 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
   137101 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
   137161 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
   137221 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
   137281 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
   137341 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtc
//
LOCUS       DAPBAB010000002       114164 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPBAB010000002 DAPBAB010000000
VERSION     DAPBAB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 114164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.002"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            173..445
                     /locus_tag="RD444_000160"
     CDS             173..445
                     /locus_tag="RD444_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547569.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            460..1383
                     /locus_tag="RD444_000161"
     CDS             460..1383
                     /locus_tag="RD444_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7547570.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1523..2275)
                     /locus_tag="RD444_000162"
     CDS             complement(1523..2275)
                     /locus_tag="RD444_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7547571.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2498..2860
                     /locus_tag="RD444_000163"
     CDS             2498..2860
                     /locus_tag="RD444_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDT7547572.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3497..4969
                     /locus_tag="RD444_000164"
     CDS             3497..4969
                     /locus_tag="RD444_000164"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDT7547573.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4981..5805
                     /gene="nadE"
                     /locus_tag="RD444_000165"
     CDS             4981..5805
                     /gene="nadE"
                     /locus_tag="RD444_000165"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDT7547574.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5878..6768)
                     /locus_tag="RD444_000166"
     CDS             complement(5878..6768)
                     /locus_tag="RD444_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDT7547575.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6966..8483
                     /locus_tag="RD444_000167"
     CDS             6966..8483
                     /locus_tag="RD444_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDT7547576.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8455..9744
                     /locus_tag="RD444_000168"
     CDS             8455..9744
                     /locus_tag="RD444_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7547577.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9919..10815
                     /locus_tag="RD444_000169"
     CDS             9919..10815
                     /locus_tag="RD444_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7547578.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10829..11692
                     /locus_tag="RD444_000170"
     CDS             10829..11692
                     /locus_tag="RD444_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7547579.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11698..12261
                     /locus_tag="RD444_000171"
     CDS             11698..12261
                     /locus_tag="RD444_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDT7547580.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12283..13794
                     /locus_tag="RD444_000172"
     CDS             12283..13794
                     /locus_tag="RD444_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDT7547581.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13806..14765
                     /locus_tag="RD444_000173"
     CDS             13806..14765
                     /locus_tag="RD444_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDT7547582.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14851..15930
                     /locus_tag="RD444_000174"
     CDS             14851..15930
                     /locus_tag="RD444_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7547583.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16182..17582)
                     /locus_tag="RD444_000175"
     CDS             complement(16182..17582)
                     /locus_tag="RD444_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDT7547584.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17800..19461
                     /locus_tag="RD444_000176"
     CDS             17800..19461
                     /locus_tag="RD444_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDT7547585.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19418..20116
                     /locus_tag="RD444_000177"
     CDS             19418..20116
                     /locus_tag="RD444_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDT7547586.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20132..21556
                     /gene="araA"
                     /locus_tag="RD444_000178"
     CDS             20132..21556
                     /gene="araA"
                     /locus_tag="RD444_000178"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDT7547587.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21863..23022
                     /locus_tag="RD444_000179"
                     /pseudo
     CDS             21863..23022
                     /locus_tag="RD444_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            23140..24999
                     /locus_tag="RD444_000180"
     CDS             23140..24999
                     /locus_tag="RD444_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7547588.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25109..25543
                     /locus_tag="RD444_000181"
     CDS             25109..25543
                     /locus_tag="RD444_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547589.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25802..25963)
                     /locus_tag="RD444_000182"
     CDS             complement(25802..25963)
                     /locus_tag="RD444_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547590.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(25982..27004)
                     /locus_tag="RD444_000183"
     CDS             complement(25982..27004)
                     /locus_tag="RD444_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDT7547591.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27132..27485
                     /locus_tag="RD444_000184"
     CDS             27132..27485
                     /locus_tag="RD444_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDT7547592.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27497..28663
                     /locus_tag="RD444_000185"
     CDS             27497..28663
                     /locus_tag="RD444_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7547593.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28755..29375)
                     /locus_tag="RD444_000186"
     CDS             complement(28755..29375)
                     /locus_tag="RD444_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDT7547594.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29567..29863)
                     /locus_tag="RD444_000187"
     CDS             complement(29567..29863)
                     /locus_tag="RD444_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7547595.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30067..30717
                     /locus_tag="RD444_000188"
     CDS             30067..30717
                     /locus_tag="RD444_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDT7547596.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30733..32460
                     /locus_tag="RD444_000189"
     CDS             30733..32460
                     /locus_tag="RD444_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547597.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32457..34349
                     /locus_tag="RD444_000190"
     CDS             32457..34349
                     /locus_tag="RD444_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547598.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            35053..35922
                     /locus_tag="RD444_000191"
     CDS             35053..35922
                     /locus_tag="RD444_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDT7547599.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            36131..37156
                     /locus_tag="RD444_000192"
     CDS             36131..37156
                     /locus_tag="RD444_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDT7547600.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            37195..38145
                     /gene="manA"
                     /locus_tag="RD444_000193"
     CDS             37195..38145
                     /gene="manA"
                     /locus_tag="RD444_000193"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDT7547601.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            38239..39942
                     /locus_tag="RD444_000194"
     CDS             38239..39942
                     /locus_tag="RD444_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7547602.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39944..41977
                     /locus_tag="RD444_000195"
     CDS             39944..41977
                     /locus_tag="RD444_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7547603.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41988..42428
                     /locus_tag="RD444_000196"
     CDS             41988..42428
                     /locus_tag="RD444_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7547604.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42628..43755)
                     /locus_tag="RD444_000197"
     CDS             complement(42628..43755)
                     /locus_tag="RD444_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547605.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43758..44435)
                     /locus_tag="RD444_000198"
     CDS             complement(43758..44435)
                     /locus_tag="RD444_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547606.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44699..46465
                     /locus_tag="RD444_000199"
     CDS             44699..46465
                     /locus_tag="RD444_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDT7547607.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46480..46917
                     /locus_tag="RD444_000200"
     CDS             46480..46917
                     /locus_tag="RD444_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7547608.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46930..48081
                     /locus_tag="RD444_000201"
     CDS             46930..48081
                     /locus_tag="RD444_000201"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDT7547609.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(48143..48841)
                     /locus_tag="RD444_000202"
     CDS             complement(48143..48841)
                     /locus_tag="RD444_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDT7547610.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48834..50186)
                     /locus_tag="RD444_000203"
     CDS             complement(48834..50186)
                     /locus_tag="RD444_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDT7547611.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50409..51809)
                     /locus_tag="RD444_000204"
     CDS             complement(50409..51809)
                     /locus_tag="RD444_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDT7547612.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51970..52707
                     /locus_tag="RD444_000205"
     CDS             51970..52707
                     /locus_tag="RD444_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDT7547613.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52832..53311
                     /locus_tag="RD444_000206"
     CDS             52832..53311
                     /locus_tag="RD444_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDT7547614.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53308..54075
                     /locus_tag="RD444_000207"
     CDS             53308..54075
                     /locus_tag="RD444_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547615.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEELVVYSRSQRRRHS"
     gene            54305..54874
                     /gene="lepB"
                     /locus_tag="RD444_000208"
     CDS             54305..54874
                     /gene="lepB"
                     /locus_tag="RD444_000208"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7547616.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54978..55580)
                     /locus_tag="RD444_000209"
     CDS             complement(54978..55580)
                     /locus_tag="RD444_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7547617.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55686..56155
                     /locus_tag="RD444_000210"
                     /pseudo
     CDS             55686..56155
                     /locus_tag="RD444_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            56261..56845
                     /locus_tag="RD444_000211"
     CDS             56261..56845
                     /locus_tag="RD444_000211"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDT7547618.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56848..57921
                     /gene="prfA"
                     /locus_tag="RD444_000212"
     CDS             56848..57921
                     /gene="prfA"
                     /locus_tag="RD444_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDT7547619.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57914..58753
                     /gene="prmC"
                     /locus_tag="RD444_000213"
     CDS             57914..58753
                     /gene="prmC"
                     /locus_tag="RD444_000213"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDT7547620.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58746..58925
                     /locus_tag="RD444_000214"
     CDS             58746..58925
                     /locus_tag="RD444_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547621.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58947..59972
                     /locus_tag="RD444_000215"
     CDS             58947..59972
                     /locus_tag="RD444_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDT7547622.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60147..61391
                     /locus_tag="RD444_000216"
     CDS             60147..61391
                     /locus_tag="RD444_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDT7547623.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61544..62491
                     /locus_tag="RD444_000217"
     CDS             61544..62491
                     /locus_tag="RD444_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7547624.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62625..63254
                     /gene="upp"
                     /locus_tag="RD444_000218"
     CDS             62625..63254
                     /gene="upp"
                     /locus_tag="RD444_000218"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDT7547625.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63393..63635)
                     /locus_tag="RD444_000219"
     CDS             complement(63393..63635)
                     /locus_tag="RD444_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547626.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63756..63828
                     /locus_tag="RD444_000220"
     tRNA            63756..63828
                     /locus_tag="RD444_000220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63789..63791,aa:Thr,seq:cgt)
     gene            complement(63947..64585)
                     /locus_tag="RD444_000221"
     CDS             complement(63947..64585)
                     /locus_tag="RD444_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7547627.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64708..65340)
                     /locus_tag="RD444_000222"
     CDS             complement(64708..65340)
                     /locus_tag="RD444_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDT7547628.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65691..65990
                     /locus_tag="RD444_000223"
     CDS             65691..65990
                     /locus_tag="RD444_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7547629.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66485..68650
                     /gene="acm"
                     /locus_tag="RD444_000224"
     CDS             66485..68650
                     /gene="acm"
                     /locus_tag="RD444_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDT7547630.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68922..69407)
                     /locus_tag="RD444_000225"
     CDS             complement(68922..69407)
                     /locus_tag="RD444_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDT7547631.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    69497..69842
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            69887..70528
                     /locus_tag="RD444_000226"
     CDS             69887..70528
                     /locus_tag="RD444_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7547632.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70547..71182
                     /locus_tag="RD444_000227"
     CDS             70547..71182
                     /locus_tag="RD444_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7547633.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            71200..72015
                     /locus_tag="RD444_000228"
     CDS             71200..72015
                     /locus_tag="RD444_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7547634.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72334..73770
                     /locus_tag="RD444_000229"
     CDS             72334..73770
                     /locus_tag="RD444_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7547635.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73959..76406
                     /locus_tag="RD444_000230"
     CDS             73959..76406
                     /locus_tag="RD444_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDT7547636.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76636..77703
                     /gene="rlmN"
                     /locus_tag="RD444_000231"
     CDS             76636..77703
                     /gene="rlmN"
                     /locus_tag="RD444_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDT7547637.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77896..78999)
                     /locus_tag="RD444_000232"
     CDS             complement(77896..78999)
                     /locus_tag="RD444_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7547638.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79552..80100
                     /gene="aac(6')"
                     /locus_tag="RD444_000233"
     CDS             79552..80100
                     /gene="aac(6')"
                     /locus_tag="RD444_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDT7547639.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            80646..82307
                     /locus_tag="RD444_000234"
     CDS             80646..82307
                     /locus_tag="RD444_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7547640.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(82377..82724)
                     /locus_tag="RD444_000235"
     CDS             complement(82377..82724)
                     /locus_tag="RD444_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDT7547641.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            82808..83749
                     /locus_tag="RD444_000236"
     CDS             82808..83749
                     /locus_tag="RD444_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547642.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            83750..84793
                     /locus_tag="RD444_000237"
     CDS             83750..84793
                     /locus_tag="RD444_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547643.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            84799..85869
                     /locus_tag="RD444_000238"
     CDS             84799..85869
                     /locus_tag="RD444_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547644.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            85859..86812
                     /locus_tag="RD444_000239"
     CDS             85859..86812
                     /locus_tag="RD444_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7547645.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            86824..87834
                     /locus_tag="RD444_000240"
     CDS             86824..87834
                     /locus_tag="RD444_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7547646.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(88119..88940)
                     /locus_tag="RD444_000241"
     CDS             complement(88119..88940)
                     /locus_tag="RD444_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7547647.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(89037..89315)
                     /locus_tag="RD444_000242"
     CDS             complement(89037..89315)
                     /locus_tag="RD444_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547648.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            89583..91592
                     /gene="metG"
                     /locus_tag="RD444_000243"
     CDS             89583..91592
                     /gene="metG"
                     /locus_tag="RD444_000243"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDT7547649.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(92071..92658)
                     /locus_tag="RD444_000244"
     CDS             complement(92071..92658)
                     /locus_tag="RD444_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547650.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            92833..94155
                     /locus_tag="RD444_000245"
     CDS             92833..94155
                     /locus_tag="RD444_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7547651.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            94310..95086
                     /locus_tag="RD444_000246"
     CDS             94310..95086
                     /locus_tag="RD444_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDT7547652.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            95079..95648
                     /gene="rnmV"
                     /locus_tag="RD444_000247"
     CDS             95079..95648
                     /gene="rnmV"
                     /locus_tag="RD444_000247"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDT7547653.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            95641..96525
                     /gene="rsmA"
                     /locus_tag="RD444_000248"
     CDS             95641..96525
                     /gene="rsmA"
                     /locus_tag="RD444_000248"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDT7547654.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            96976..97605
                     /locus_tag="RD444_000249"
     CDS             96976..97605
                     /locus_tag="RD444_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547655.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(97729..98838)
                     /gene="ugpC"
                     /locus_tag="RD444_000250"
     CDS             complement(97729..98838)
                     /gene="ugpC"
                     /locus_tag="RD444_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDT7547656.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            99047..99469
                     /gene="mgsA"
                     /locus_tag="RD444_000251"
     CDS             99047..99469
                     /gene="mgsA"
                     /locus_tag="RD444_000251"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDT7547657.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            99792..100328
                     /locus_tag="RD444_000252"
     CDS             99792..100328
                     /locus_tag="RD444_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDT7547658.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(100368..102131)
                     /locus_tag="RD444_000253"
     CDS             complement(100368..102131)
                     /locus_tag="RD444_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547659.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(102134..103849)
                     /locus_tag="RD444_000254"
     CDS             complement(102134..103849)
                     /locus_tag="RD444_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547660.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(104628..104852)
                     /locus_tag="RD444_000255"
     CDS             complement(104628..104852)
                     /locus_tag="RD444_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547661.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            105043..107820
                     /locus_tag="RD444_000256"
     CDS             105043..107820
                     /locus_tag="RD444_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7547662.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            107901..108320
                     /locus_tag="RD444_000257"
     CDS             107901..108320
                     /locus_tag="RD444_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDT7547663.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            108332..108802
                     /locus_tag="RD444_000258"
     CDS             108332..108802
                     /locus_tag="RD444_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7547664.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            108819..109598
                     /locus_tag="RD444_000259"
     CDS             108819..109598
                     /locus_tag="RD444_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7547665.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            109585..110406
                     /locus_tag="RD444_000260"
     CDS             109585..110406
                     /locus_tag="RD444_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7547666.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            110423..111472
                     /locus_tag="RD444_000261"
     CDS             110423..111472
                     /locus_tag="RD444_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7547667.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            111485..112489
                     /locus_tag="RD444_000262"
     CDS             111485..112489
                     /locus_tag="RD444_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7547668.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            113122..114141
                     /locus_tag="RD444_000263"
     CDS             113122..114141
                     /locus_tag="RD444_000263"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDT7547669.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        37901 a        19802 c        23440 g        33021 t
ORIGIN      
        1 cattcactaa taataaagat atatttacag tatcattata atgtattcaa tttaaaataa
       61 gagactttgg taataatgaa agaggaataa aacttttctt tggcaaaaca aactccttgc
      121 tatgagacgc gtttcataca ctatactcaa agtatcaaaa ggagggcttg caatgaatac
      181 ttatgtagag ataacagatg gcacaacgaa caattatttt tatgcgtttg tcgagttcaa
      241 gggatccgta ttaacgttgt acagttttca agggttgaat aggaatatag taaaagaaat
      301 accaatgaat gaaatcgaaa atttgacaaa agatatatat tggggcgggc agcggatcag
      361 tttttcgcat gaaggaaaaa tgtatcagtt ttttgaatgc ggtccagctg tggtagacta
      421 tttacaagaa aatctttttg tataggtggc gaagcagcag tggcaaacat tcgagatatc
      481 gcaaagctca caggttattc agtttctact atttcacgtg taatcaatgg acatccatat
      541 gtagatgagg aaaaaaggaa agaaatactt gcggtcatga aagaagtcga ttatatccct
      601 aatgcaaatg ccagacagtt aagttatgga aaaacgaaaa gcattggagt gattctgcct
      661 tataccaatc atccgtattt tgatcagttg atcagtggga tcattgaagc agcgtttgat
      721 tatgattaca aagtgacact tttaccgacg ggctatcata aagaaaaaga gcgaaggtat
      781 cttgaagaat ttgcagctaa agctttcgat ggattgatca ttacgtctcg tgccaataca
      841 ctagatgatt tattggcata tcaaaaatat ggacctctag ttttttgtga agaaatcaag
      901 ggaaaacagg caagctgtgt ctttattgat cgagaacaat caattacaga aggtcttact
      961 tatttgaagc agcaaggtat aaagcaattg ggcatcacgt tggggcgaag tggcaggcta
     1021 agttataact caaaaatcac tttgcagcta tgtcatcaac tttttccatc ttttgatgaa
     1081 tcacttgttt tttgggattg tatagatgca gagaaaggaa aacaagcagg cttctttttt
     1141 aaagagcttg gagttgatgg tgtctttaca aattcggata tggttgcagc aggcatcttg
     1201 caaagctatc tgcaagataa gacaccagtg gtgattggac gcgataattt gctgatcagc
     1261 gagcttcttg gtttcccaac catcgaccac catctggaag cttgcggaga aatggcattt
     1321 caattgtttc ttacagaaaa aaaggataaa gtgaaaatac cttatacgtt tattcaacga
     1381 tgaatatatt taaagtagga tgaaaaaact tgtgaaagaa tggagcagac accaaaagtt
     1441 agcataaaaa tctaacttgg gtgtctgctg ttttgttatt aggtcatttt acgtgtactt
     1501 ttttgttgga tcatgtaaaa gattacgcct ttttttggat aatttcattt aaagcttttg
     1561 ctgcaccatg accacttcgc tcgtcataat atgcagtgaa ccgttcatcg gcttcgtaca
     1621 tcattcctag tcctttatgc gcttttggct gatattcagg ccagctgtaa gagaaccact
     1681 tcttatgagc ttggaagata cggtctgcta agctttcatc ttctggttct tttaagtaaa
     1741 gagtgagatc aaataacatc tgcttttcaa cagtttgcat ttcttcatac gtttctttat
     1801 ccatattcat ccatttttca ttggatgctg taaccttttt ttttccgtat ttttgtcgaa
     1861 tctcttgtcc ataggattct tcattttctt tcaacttttg ttgtttaaac ccttcaaatt
     1921 tttctttgtc actcattttt cttcctcctt cgtacatatc aagtgtctgt tggacagttg
     1981 tcaagagggt atcgatttgt gcccgttttt ccagcaacat ttgctgatgc ttgattaaag
     2041 ctttctggtg gtcaaaagat ggattgtcta agacctcttg gatatcatcc aacttgaatg
     2101 ccattgttcg ataaaataag atgtgttgca gacgatcgac ttcttttttg ccataaatcc
     2161 gataacctga agaattgatc ctagctggtt ttagcagtcc aatctcatca taaaagcgca
     2221 aggttctcgg actgactcct gatatctctg acattttctt tattgtgtac tccattattt
     2281 ttcctcctga caaatgtaag cataatcctt tacgtaacga gatggtcaag aaaaaattgt
     2341 atttttttct cacgaaatta tgttatacta ttaagtgata aaaggtaaat ccttttgtct
     2401 ttaaaggttc aattgtaacc gattccttaa tgctaggaaa gatggtaaca taacttaaga
     2461 ttggatctaa atcgactata tagaaaagag gaatgagatg aagctaatcg aaacacctgt
     2521 gaatagtaat ttaaatatta agacatttta tcctaaagta gtggaattct tttttgggaa
     2581 tacggctatt aattattaca aattattttc gttagatcga acacaactgc tattggtaga
     2641 cacatatgat aaaaagcaag tcgtgatgat caatacgaag aaaaaaatca caaggcaaga
     2701 aatcgactat gcgattcatc acgttttaaa aatgacacga gaagatgtca aagtccatgt
     2761 aggagtgaaa caggagctag aacgtgccgg aatacaattc aaacgtccaa ataaagatat
     2821 tgtagtgata gaacaaaaaa atacgaagga ttctatttaa taacgactaa taatcatctt
     2881 tatggtaacc tgctccatat ctaatgtata caataaaaaa caaggcgaga ctgattgatg
     2941 tctcgccatt tttgtttcca ttctattgac agctgctttc ttactttgta tctttttaaa
     3001 aacgtgtgat attttttccg cgagtttttt caacgcaagg tatatcttgt cataattcgc
     3061 ctttccaaaa aaattcaata cataataagt tatatccgtt tctttgggga taagaagcaa
     3121 aaaaggaaag acgtattatt gcttcaaaaa cttttgccat tgattatgtt gtagagacat
     3181 ttggttatat ttgttcaatc atcggtatct taacggctac gattgtagta ggaaaatcta
     3241 cttattttat caagtaagaa aaatagaaaa cgctaatttc ttcgtgtctc ggatacattg
     3301 aataaaataa gtattctttc tcgtttaggt taaataccaa taagaaagga tagttttagc
     3361 agatggagtt catttttagc aagaaatatc cgtaaaacat gtgctttttc agaaaaaatg
     3421 gaagatttct tttcctatta attttttttg ttataatggg taggaaatga aaacttgcca
     3481 agaggagtaa tacaagatga ataatactta cccagacgac agtttgacgc tgcatacgga
     3541 tctatatcaa atcaatatga tgcagacata ctgggaatta ggtcgagctg atttacatgc
     3601 tgtttttgaa tgttactttc gagaaatgcc ttttaaccac ggatacgcgg tttttgcagg
     3661 attggaacgg ttagtcaatt atttagagaa tctgacgttc aatgattccg atattgctta
     3721 cttgcgtgga ttagaagtct atcctgaagg ttttttggag tatttacaaa attttgaatt
     3781 taaagcaaca gttcgttctg ctcgtgaagg agaactagtt tttgcaaatg aaccattaat
     3841 ccaagtggaa gggcctttag cgcactgcca attagtagaa acagcactgt tgaatatggt
     3901 caatttccaa acgttgatcg cgacaaaagc agctcgtatc aaatctgtca tcggagaaga
     3961 tccactattg gaatttggaa ctagacgggc acaagaactg gatgcagcag tctggggaac
     4021 tagagcggca tatatcggcg gagcagatgc aacaagcaat gtacgtgctg ggaagatatt
     4081 tgggatacca gctagcggga cccatgctca ttcgttagtc caatcttatg gaaatgatta
     4141 tgaagcattt atggcctacg ctaaaaccca taaagactgc gtgttcttag tcgacactta
     4201 tgatacgttg aaatcaggtg tgccaagtgc gattcgcgtg gcgaaagaat taggtgacaa
     4261 aatcaacttc caaggcgtac gcatcgacag tggagatatg gcctatatat ctaagcgtgt
     4321 tcgggaacaa ttagatgcag ccggatttac ggaagcaaaa atctatgctt cgaatgactt
     4381 ggatgaagca acgattttga atttaaagat gcaaaaagct aaaattgatg tatggggtgt
     4441 tgggacaaaa ttgatcacag cctatgatca gccagccctt ggtgcagtat tcaagctagt
     4501 atcgatcgaa gatgatgaag gaaaaatgat ggatacgatc aagttatcaa gcaatgcaga
     4561 aaaagtaact actccaggga aaaaacaagt gtggcgcatc actcgaaatt ttgatgggaa
     4621 atctgaagga gactacgtga cattatggga tgaagaccct cgagaagaag aagctatctt
     4681 catgttccat ccagtgcata cgtttatcaa taaaactgtt cgagacttta cagctcgccc
     4741 agttttacaa gatatcttta tcgaaggaaa aagagtttac gaattacctg cactaaacga
     4801 aatcaaagaa tatacaaaag aaaatctaga ttcactatgg gaagagtata aacgagactt
     4861 gaatccgcaa aaatacccag tcgatctgtc tactgaatgc tggaaccata aaatggctat
     4921 catggaacga atgaaaaaaa gcgtagcaga acttcataca gaagcatagg aggagaataa
     4981 atgagcctgc agacagaaat tatcaatgaa ttaggtgtca aaccaacaat tgatccaaaa
     5041 gaagaaatac gtaaaagtat tgactttatg aaagcatatt tgaaaaaata tccgtttctt
     5101 aaaacttttg tactaggtat cagcggcggc caagattcta ctttagcagg ccgtttggcg
     5161 caactaacga tggaagaaat gcgtgaagaa acaaaagatg aaaactatca atttatcgct
     5221 gttcgtcttc cttatggtga acaagcagat gaagaagatg caaaagctgc tcttgacttt
     5281 atccagcccg atgttagtct gcgagtaaat atcaaaccag ctgttgatgc acaagtacaa
     5341 gtattaagcg aagcaggtgt gggaatcagc gattttaaca aaggaaatat caaagcacgt
     5401 caacgtatga ttacgcaata tgccgtagct ggagaaagag caggagccgt attagggaca
     5461 gatcatgctg cagaaaacat tactggattc ttcacgaaat ttggtgatgg tggagccgac
     5521 attttgccat tattccgttt ggacaaacga caagggaaac aattgcttca agcattgaat
     5581 gcaccggaaa aattatacac aaaaattccg actgcagatc ttgaagatgg gaaaccaatg
     5641 attgcagatg aagtcgcact aggtgtcaca tataatgaaa ttgatgatta tctagaagga
     5701 aaagaagttc ccgctcaagc acaagaaaaa atcgaagcat ggtggaacaa gacacaacat
     5761 aaacgacatt tgccaatttc cgttttggat gatttctgga aataacaata aagcaaaaac
     5821 agccgtccct tcatagggat tggctgtttt tgttttataa aatagaaaag aaaaatatca
     5881 gctgtagtaa acttttaatt tgatatcctg atcgtaatta ccgaatcctt taccaaataa
     5941 agtacagccc cccacatgtt tggcatcgtc tttcacttcg attcgcaaat gccaagtgtc
     6001 ttgctcttta gggatgtcgt tgatcgtgat gtttgacaag ggttctccat tcatattcgt
     6061 caagtgtttg gtaattcgga tcgtcttcag caagccgtat tgattaagat tatctggata
     6121 ccaatcaggc gtatatttac cgcggatatc agcaaaatct ccagggctcg tccatgttcc
     6181 tagctccaca ccatttaggc tgaaagtgat atctgaaggc caattgtcat ttgaaaaagg
     6241 aaattcagaa gaaatttcca cagaaacttc caacatctcc aatgtatcct ctgtatttaa
     6301 aaaattaggt gcctgatact cgacaaatcc gctggtaaac cagagaatgc gggcatccat
     6361 ccttcgagga tccataaaat agcgtggttc atccactttc ccgataaaat catggatcgt
     6421 agctaaaccg catgttggtt caattgcata atttgtatag tgaccaatag gtatcgatgt
     6481 ttctttcgta tcaaaagcat tgaatatttt ttctggaaaa ctgatatcga ttttatcgac
     6541 acgaaggctc gaaatttttt gttgtcctac tttttctgtt ttgatgatgt ttgcttcctc
     6601 tagctttttc acgtgcatcg tcgtgatagc actgctcact ccaagttctt ttgccaaatc
     6661 tttgacattc atcttttttt tggaaagtag ctggatgatc ttgatccttg ttttactagc
     6721 taatgcttca tataccggta aggattcttc gtttatttgt agttgcatat atatcacctc
     6781 ttaaaaggtt tttcttatat attaacatat ttgttaataa aagttatatt aaattatttg
     6841 ttttaatcat tattaatatt ttggttaatt aaaatcgata aaaaataagt aagtttaaca
     6901 tttatattat tgacaacgct ttcaacgagg tgtattattc atgtataaat taaaggaggt
     6961 cagttatgga agccacaatg ttgattagta gtgtcaagcg tgtaggcaaa gttgatccgc
     7021 ggttgtacag ttcatttatt gaacatatgg gacgagcagt atatgaaggg atttatcagc
     7081 cagatcatcc atcagctaat aaaagtggat ttcgccaaga cgtcattcac ttggtaaaag
     7141 aattgaacat tcccatgatt agatatcctg gtggaaattt tgtgtcagga tttcgttggg
     7201 aagacagtat tgggcccata gcaaatagac ctagaaggtt ggatttagct tggcggacaa
     7261 ttgaaacgaa tgaagttggg attcatgaat tttataactg gtgtcagcat gtgggggccg
     7321 aaatcaatat ggctgtcaat ctgggtactc gtggagtaga tgcagctcgt aatttagtcg
     7381 aatattgtaa ttttcctaaa ggaacttatt ggagtgattt aaggcgttcg aatggtcaag
     7441 agagcccttt tggaattaag acatggtgtt tagggaatga aatggacggt ccttggcaga
     7501 ttggacataa aacagcggaa gaatatggac ggttagcaga ggaaacggct aaggcgatga
     7561 aactcgtaga tgattcaatt gaggtggttt tatgcggaag ttcgaatcgg caaatgccta
     7621 catttggtga ttgggagtta acagttcttg atcaagcgta cgatcaaata gattatttgt
     7681 ctctgcatca atattatggc aaccaaaaaa atgatctttc tcattatctt gcacgttcct
     7741 tagacatgga tcggttcatt tctgaagtga cagcgatgtg cgatacagtc aaggcgaaga
     7801 aacatagtaa gaagcagatc aacctgtcct ttgatgaatg gaatatttgg tatcattcag
     7861 cggaacaaga tgagcaagca gcgccttggc agattgctcc tcctttacta gaagataact
     7921 ataattttga agatgcatta ctattaggct gtctattaat cactttattg aaacatgcag
     7981 atagagtaaa gattgcttgt cttgctcaat tggtaaatgt tattgcacca atcaggacag
     8041 aaacaaacgg agaagcttgg agacagacga tctattatcc atttatgcaa gctgctgtgt
     8101 atggacaagg agaggtgtta agcccacaaa ttcgttcagc aaactatgcg acggaagatt
     8161 ttgaagaagt gccgtattta gaaagtatag ccgtaataaa tgacaaagaa cttgtcatat
     8221 ttgcagttaa ccgtggagaa gaagaaatga atctaactgt tcacttgtct gaattatcat
     8281 tagagggagt gattgatttt tctgaaatga gtggatttga tacaaaagca actaatcttc
     8341 ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt aaaagctgaa gatgggaaaa
     8401 tcaattgttt attgaaaccg ctatcttgga atgtggtcag atgtcgttta tcagatgaag
     8461 gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct ctttgttggt agctgcaaca
     8521 ggtattttag gagcttgcgg tggcggaagt tcagcagaat ctgattctga taaagaagtg
     8581 actttgactt tttggaatgg gtttactgca tctgatggtg aaatattgca agaaatcgtt
     8641 aatgaattta ataaaacaaa tgataaaaac atcaaaattg aaatggatat catgacttgg
     8701 gccaatttga atgaaaaact tcctacagcg atttcttcta aaaaagcacc tgactttatt
     8761 gcattaaatt atccagattt tgcacagtat gtagaaaacg gagcggtaca accattagac
     8821 gatttctggg aatatgatgg agtagataaa tctgatttta aagaaacggc tattgaatta
     8881 ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg tacaaggcat gtatatgtat
     8941 tggaataatg atttgtttaa acaagctgga ttagatactg aacaaccacc tactacttgg
     9001 gaagaattga cagaaatggc accaaaatta actgatagta gtaaaaatgt atcaggtttt
     9061 gtcttcaata aggatggaac tgcaccgtta tataactgga tgttagcaaa tggcggtaaa
     9121 ttagtgaacg atgattatac taagtctgaa tttgcgtctc ctgagaacct taaaacgtta
     9181 aaagcgatcc aaaaaatgat ccatgtagac aagactggtc cagaatcgat ttctggcgcc
     9241 gaaatggaca atttaatgaa tgctggtcaa ctagccattg aaatcaatgg gccatggtta
     9301 aataacggat tgaaagcaaa cgagattgat tataatgtga ctaccttacc acaagggagt
     9361 gattcagaca aaaaaacagc tattttagat ggcgtaggat atgccattcc ggcaagtaca
     9421 gataataata aaaaagaagc tgtctatgag tttttgaaat attggaatac aactgaaatt
     9481 ggtaaaaaat ggagtatcga aaatggtttt cctgcctact tggattcagt agcaaaagat
     9541 gaagaagtaa aaaatgatcc tattgtatct gaattatcga aacaaatgga ctatgcagag
     9601 ccatatttac caggttttac ccaaatctct gcgatcaata acgatatcat caatccactg
     9661 attgaaaaat tattagcagg agaagatcct gagacactca tgaacgatgc agatgatgcc
     9721 atcaatcaat tactgcaaaa atagtacaga actttataca ggatagactg ttggcaagat
     9781 acagatatct gaatgatcga cctagtattt gcagataggt gttattgcaa aaatcacagc
     9841 gattgttcgg atatcttatt cagattcgtt taaaccaaga ttgtatattt tatcaaaatg
     9901 aaagatgagg tggactatat gaacggattc accaaatatt ggaatcgtcc tgatcgtgct
     9961 gcgtatttgt ttttaactcc atctttttta gttttattat tatttactgt cataccgctt
    10021 ttaggaacat ttgctattag ttttacagat ttgaatattt tctttacatc aagaaatttt
    10081 gtaagttttg aaaatttcac acgtatattt agtgatgaac gagcagttaa ttcgttgatt
    10141 cattctttat atttcaccgt actcgaaacg ccaacacaaa ttatcgttgg tttattagca
    10201 gcagtagcat tatctaaaaa taccagattc aatcgttttt gtcgttcgac attttacatt
    10261 ccagttattt gttcattgac gtcaattgcg attatttttt ctatgttgct tgatccgaat
    10321 gtaggggcga ttccttactt gttttcacaa gtcggcttgc cgatccctca attttttcgc
    10381 gatccaaccc ttgctatgcc gacggtagcc atcatgactg tttggaagaa ttttggtgtt
    10441 actatgacga ttctcttgac tgctattcag ggaatctctc cctctttgta tgaatctgct
    10501 caaatggatg gtgcaacaaa tcgacagcag ttttttaaca ttacgctacc tcagatcgtt
    10561 ccctccttag gtttttgtat tttaacgaat ctgattggtt cgatgcaagt gtttgaccaa
    10621 gtatatgttg caaccgctgg tggcccacaa tttaagacag aaacagctgt tcaatatatt
    10681 tacagtcgag gatttacagc accttatgaa ttgggctatg catcagcact gtcttcggtt
    10741 ttgtttatca ttgttgcaac tatagctatc tccactaatg tgtacatgtc acggaaagaa
    10801 aaacaaatga aataggaggg aaacctgtat ggcaccagaa agctcgatta cagttactgt
    10861 gaatagtcga aaagcagtta gtggtaaaaa aatcattcga acgattagta tgctagttat
    10921 attgttggtt attgcgtttc cttttttgtg gctgattatt tcttctttta aacacgaaaa
    10981 agacattatc tcatttccgc ctagaatatt tgcagatagc tataccctag ataactatat
    11041 taaggtttgg acaacgatcc ctttattaga ttacatcaaa aatacagtga tttttgctgg
    11101 agggacagtg atcacttctg tattctttga ttctcttgcg gggtatgcat ttgctcgtat
    11161 gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg ctgacaatga tgatcccatt
    11221 ccaagtcttc atgattcctc tatttattga agtcaatttg cttggcatgc ttgatactta
    11281 tgcaggtttg atcattcctc gtatgacaac tgcatttggt atcttcatga tgcgttcctt
    11341 ttttattact ttaccagatt ctttggaaga ggcagcacgc attgatggat taagtgagtt
    11401 taatattttt ttgaaaatca tgttgccatt gagcaaacct actcttttat cattagggat
    11461 tttcacgctg atgaatagtt ggaatgactt gctttatcca ttgatattga caagtagttc
    11521 aaaaatgaga actttaccag cagggttagc tttatttaca gggcagaaca tttcttttta
    11581 tggacctgta atggctggaa cggttatttc aatgctgcca ttactagtgg tttatatttt
    11641 tgcacagaaa tattttgttc aaggaacagc tatgtctgga atgaaagagt gaggaagatg
    11701 aaaatagttg agactaaact ttatggacag ttgattgtgg tttctgatta catcttatta
    11761 ggtattctct gggtaatcgt gtgtttgcct gttattacta tcgtgcctgc tacatgtgct
    11821 gttttttatg taatggcgca atggaaaggt gagggatcag gaaatatcat ttattttttc
    11881 tttcaaggaa taaagcaaca ttttggtaag aacttgttcc ttagtctgtt aacgatcatt
    11941 atttattttc ttgttaatca attattagaa gaacagacaa tcgtactttc aattagtgga
    12001 tatgtagttt tacttattta ttccatgttt cttttatcct ggatatatgt gatttctgaa
    12061 aacaaagata tcacattttt acaaattttt gaacaaagtg catggaaagt attttttgat
    12121 tttttgagaa atattctttg ttgcgtattg ttattaggat ttgtactgct catatttctt
    12181 ttccctcctt ttatttttat ctttgctggt ggcgtatgga aacttgtatc tattatcttg
    12241 actataggaa atgaaaggta gggaaagctg ttggacgaag aagtgaaagt aaaaatcaga
    12301 gaggatttta cggtaggaga gatcagttct cggctgtttg gctcatttgt tgagcatatg
    12361 ggaagtgtcg tctatacagg gatttatgaa ccgggccatg aaacagctga tggtgacggg
    12421 tttagacaag atgtcctctc ttatgtaaag gaattagaat taggtgtcat tcgctatcct
    12481 ggaggaaact ttacatctgg ttatgattgg gtggatactg taggtaggaa agaacaacga
    12541 ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa attcttttgg actccacgaa
    12601 ttttttaaat ggatcagtca tgtacaagcg aaaccgatat tcacggtgaa tttaggaaca
    12661 aaaggaattg atgaagcaaa aaatttagta gaatattgta attttccaaa aggaaccttc
    12721 tggagtgatt taagaataaa aaatgggcaa aaaaagcctt ttggcgttaa attatggtgt
    12781 ttagggaatg agctagacgg tccatggcaa gtgggggcta agagtgctta tgaatatggt
    12841 aggttagcaa atgaagcctc aaaagtcatg aagttagtgg atgattcaat tgaaacaatc
    12901 ttagttggaa gctctacgcc acgtttacca tcgtaccctg aatgggatcg tatcgcatta
    12961 gaagaagctt atgagaatgt agattattta gctttgcata actatattga taagtatgat
    13021 gaagaagact tgactaaacc tcctaaaaat gagcgtgatg atacagcgac ataccttgca
    13081 aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg ctacttgtga ttatgtaaaa
    13141 ggatatttga gaagtgagaa aacaatgtat ctagcgtttg atgaatggaa tgtacatgcc
    13201 caaccggaaa aaagacatag ggattttgaa gtcggtagtc ccatcgattg gtgttacttt
    13261 actatggaag atacattatt atttggttca ttagggctgg ctattttacg acacgcagat
    13321 cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa tacctttaat actgacagat
    13381 gctaaaggac aagcatggag gaatccaact tattatgtga tgcaacactt atcgaaatat
    13441 gctaaaggtc aagtattgaa acaacaaatc atttgccctg tttatgattg tgaaaagata
    13501 aaaaatgttc cttatgtgga cagtgtgatc gtagataacg gcaatcagct gactattttc
    13561 ttgatcaatc gcacaaatga acaacaaaag ataacgattg aacatgattt caatttgcta
    13621 gatcaaggag agcactacac attaaccagt agcgatctgt tggacaaaaa tacacgtgaa
    13681 gaaccagaaa aaataaaacc tgaaataaac tatttttcaa tagaaaaaca tgacgaatta
    13741 aatgtaggga tagaaaaata ctcttggaat gtaattagaa taaagaaaat gtgaggaaat
    13801 ttgttatgat gaagacactt tataataatc ctatcgttcg gcaacgggca gatccttggg
    13861 tatataaaca tacagatggt tattattatt ttacaggatc ggttccaggc taccaagtga
    13921 ttgaattaag aagggcaaag tcactgaatg aattagaaca tgctgcaagc ctgattgtat
    13981 ggcaggctca tgaagaagga ccgatgagtg aattgatctg ggcacctgaa gtccattata
    14041 tcaatggaaa atggtatatt tactttgctg caagcaatca taaaactata cgtgatgaat
    14101 ctcatcatca ccgcatgttc gtacttgaaa atcagcatat agatccaatg aatacagatt
    14161 ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt cagtttagac ggaacaactt
    14221 tctcttctca aggaaagcta tattatgtgt gggcccaaca agatcctgaa attactggga
    14281 attctaatct ttatctatcc gaaatggata atccttggac gttaaagggg aagcagatcc
    14341 tcctttctat acctgagtat gaatgggaaa aacggggttt ttcagtaaat gaaggtccag
    14401 cagtccttac tcgcaatggg aaaatattca ttacgtattc tggtagtgca acagatgaaa
    14461 actatgccat gggcctgctg tgggctgatg agcacaagaa tcttttatta aaagaatcat
    14521 ggcataaatt atcggagcca gtattcgttt cttctgaaaa aaataagcaa tttggtcctg
    14581 gtcacaactc gtttacggtt tcagaagacg ggtgctttga tattttgatc tatcatgctc
    14641 gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc taatcggcac gcgaacgcgc
    14701 aatgctttac gtggaatgag caaggatttc cagaatttgg tgaacctgtt ccgtatgcct
    14761 tatgagcaca agaaaccttg atttttgctt gaataaacct tgcagactcg ttatgatata
    14821 tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat atcaagatat tgcagaagag
    14881 ataaaacaaa agatccttcg tgaggaatac gaagtaaata caaccattcc ctcagaattc
    14941 aagctgcaag aaatatatga cgtcagcaga catacgattc ggcaagcaat cgctgtgttg
    15001 gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca catatgtaga tgacagctat
    15061 aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg gtgtcatcac cacatatctc
    15121 tctgattata tttttccgtc gattatccga ggaatcgaaa atgaattacg agaatctggg
    15181 tattcattgc ttttagccag tacgaataac gatcacgaat tggagaaaga gtgtttgaag
    15241 aaaatgattg cttatggggt agatggtttg atcgttgagc ctacgaaaag taatcagtat
    15301 aacccaaact tagcacttta tgttgctttg agagaacaag gcattccgat ggtgatgatc
    15361 aatgctgctt atgaagagtt aaatgcctct tatatttgtt tagatgatgt gaaagtcggt
    15421 tttatggcag cagaatattt gataaaaaaa ggacatcagc gtctgttgtt catcacaaaa
    15481 atcgacgatc ttcaaggaaa atacaggatg aaaggtttta tcagcgcgtg tgaacagtat
    15541 aatgtgcgtt ttttgacgga agatatcatt actttcacca cggaaacacg agaaacaatc
    15601 gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt tagatggcat catttgctat
    15661 aacgatcaaa tagctagttt aatgctagat aagttgacag aaaatggcta tcatattcca
    15721 gatgacctat cgatcgtagg aacagacgat tcttctttaa gccatttagg gaataaaaaa
    15781 ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag atgccgcacg gtggatcgtc
    15841 acaacgattg aaaaaggaca ggcacagcca aatattctct atgaacctgt actagttgaa
    15901 cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt tttaccgcct cttattgctc
    15961 ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca tcgctcaagg ctgaacgttt
    16021 ctttcaccac ctctccacta aacggtgttc tcctaccaac tggtcacaag tcacaatatt
    16081 catcaaacat gagaagctgt tttttgaaat tgttccttgt gactggaaac taagcggttc
    16141 cttcttattt cttgaagctg accacttctg tcacaacctc tttaagaatg atggatagaa
    16201 atacgtttag gataagtttt tttagatgca ctttgttcga tttcctctaa agatttccca
    16261 cgagtttctg gtacgatctc ttgaataaat aaaaccccta aaaagcagat gatcccaaaa
    16321 atgccgaaaa cgatttcttg agacatagaa gcagtcatca caggaaaaag caagccaact
    16381 aggaaagaac caatccaatt aaaagaagag gctaggccag atgctcgccc gcggacagct
    16441 agtgggaata tttcgcctac gattacccat gtcagtggag cccatgtgca agaataaaat
    16501 gcgacataaa tacataaaaa caagagaatc aacaacgaat ttgtatgagc atccagtaca
    16561 gttcctaaaa cagctggtag gatgaaagaa agtcccatca cgctaccgcc tattttcaat
    16621 aaagttcgcc gattgaactt gtctgcaata accaaaaaga gcaaagaacc agcaactaaa
    16681 atgattcctt ggatgatcgg ccacatcaat gcatcgctcg ctgcatgccc agtcgctttt
    16741 tctacgatca gcggaatata gtaaaaaata gcattagctc cttgaaattg ctggaatact
    16801 gctacaccaa taccggcaat gactaaggaa cgatattttt ctaaaaacaa agtatgccaa
    16861 gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga tttgttcgaa ttcctgttca
    16921 gcttcttttg gtgtccgaat atagttcaat actctttttg cctcttccaa gcggccagat
    16981 tgcataagaa aacgcggtga ctctggtaga gctaaaacac cgaaaaataa aatcagcgcg
    17041 gggacagcag cgagccctaa catcacgcgc caagccatcg tttcaggcaa cccttttagc
    17101 aaataatctg caacatagga aaggagcatg ccagaagcaa tcatcacttg attgatccct
    17161 gacaatcttc cccttagtct agcaggagcc atttcagaca tataagcagg aactagggca
    17221 gatgcagcgc caacagctaa acctagaaga atccttgctg caatcagaaa ataagaacca
    17281 ttgtgagggg cagatgcaga aaggacagaa ccagccataa aaataagtgc agaaagaaga
    17341 atcatttttc gtcgtccaag cttatcagaa agaagaccag ataaagagcc gccaaggatc
    17401 gcacctaaca tgaccgaaga cgtgatccat cctatcaaag aagctttacc tgccaaaccc
    17461 caatcatgct gtaaaaaagg taaggcgccg gtcataacac caatatcata tccaaataga
    17521 atgccgccaa atgcgccaaa aaaataaata aacgatgagg aaactttttt ttcttttttc
    17581 ataagtattc tctccttcta gaatttcgag aaggcggttg atcagatttt gtaagcaatt
    17641 tcattttata acttatgtac gtacaaaaca agtgtttttt attgtattct tgtaaataac
    17701 tgcttgacat gtacgtacaa aagtatacaa taatcattgt aagcgctact aaaaagacgg
    17761 tttgatcagg tttgacgaga aggagggagt taaatcatta tgaaggaagt aaatgcgcaa
    17821 agtatgttag atgtcataga tggtcgtaca tctttaggga ttgagtttgg atcaactagg
    17881 atcaaagctg tattgattga ttcatctttt actcctattg ccgctggtag ttatgagtgg
    17941 gaaaacaaac tagaaaatgg tatctggacc tattcattag aagatatttg gaaaggactg
    18001 cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa tctatggcga gaatcttacg
    18061 acaatcgggt cgattgggtt cagtgcaatg atgcacggtt atctagcatt tgatagcaag
    18121 gaacagttac tagtaccatt tcgtacttgg cgaaatacaa tcactgaaga agcagaacga
    18181 atattaacgg aaaaatttca atacaatatt ccgcagagat ggagtatcgc tcatttgtat
    18241 caatctattt tgaataaaga agaacatgtg aaagaaatta attttttgac aacgcttgca
    18301 ggttatgtcc attggcagct tactggtgag aaagtactag gcgtaggaga tgcatcaggc
    18361 atgtttccta tcgatccttc aacgaaaacc taccatcaag aaatgaaaaa tatttttggt
    18421 cagttaatcg aaaaagcgca cgtagcgatt gacttggatc acattttgcc taaagttcta
    18481 gtagcaggag aagaagctgg caagttaagc gaatcagggg cttatctgat cgaccctacg
    18541 ggaaatttga gaccaggaat cccggtttgt ccgccagaag gggatgcagg aaccggtatg
    18601 gtcgctacga acagtgtagc caaagggaca gggaacattt cggctggtac ttccgccttc
    18661 gctatgatcg tattagaaaa agaactagaa aatgtccatc cggaaatcga catggtcact
    18721 acaccttcag gagaactagt aggcatggtt catacgaata actgctcttc ggatatcaat
    18781 gcttgggtca gtatttttga agaatttacc aattctttgg gtatggagat aagcagagat
    18841 aaattattca cggtattatt caacaaagcg ctagaaggcg atacggattg cgggggatta
    18901 ctttcttatg gttatttttc cggtgaaaac attacaggag taaatgaagg aaggccatta
    18961 tttgtacgta caccaggtag ccgttttgat ttagcgaatt tcatgcggat ccatttagcc
    19021 agtgcttttg gtgcaatgag aatcggaatg gatatattga aatctgagaa tgtccaaatt
    19081 gatcgtttag tggggcatgg ggggattttt aaaacaccag aagttggaca gcggattcta
    19141 gcatctgcta tggaggcacc agttacagtg atggacacag ccggagaagg tggagcgtgg
    19201 ggaatcgcgt tattagcagc ctatatgatg gatcgacatg ggaagtcttt agaaaacttc
    19261 ttggcagaag acgtgtttgg acaagaccaa ggaatcacca tcacaccttc agcagaagaa
    19321 atagcaggct atcagatatt tatgagacgt taccgtgagg ggatcgacat tgaaaaaaca
    19381 gcgattcatc aattgcaaga aaaaggagtg atagaaaatg ctggaacaac tgaaagaaga
    19441 agtataccaa gcaaatcttg acttgcctaa acacggactc gtcaaatata catggggaaa
    19501 tgtgagcgct tttgatcctg aaacacgata tttcgttatc aaacctagcg gagtcagtta
    19561 tgaagagatg acagcagacg acatggtcgt tgtggacttg gacaatcata tcattgaagg
    19621 taaactcaat ccctcttcgg atacgcctac tcatgctgtt ttatatcgtt catttcctca
    19681 aatcggagga atcgtccata ctcactcgac atgggcgacg atctgggcac aagcaggatt
    19741 ggatgttcct gcgatgggga cgacgcatgc agatactttt tatggatcgg taccttgtgc
    19801 tcgatttttg acacaacagg aaatcgacag cgggtacgaa tatgaaacag gaaaagttat
    19861 catagaaaca ttcaaagaac gcaaaattga tcccttagca attccagggg tacttttaca
    19921 cggacacggt ccatttactt gggggaaaga tgcgcaaagt gcggtaatga acgcagtcgt
    19981 actagatgaa gtatgcaaga tgaatttatt taccagacag ctcaattcat tttcagaaga
    20041 gctgcctcaa cggattttag ataaacatta tttacgaaaa cacggaaaaa atgcgtatta
    20101 cggacaaaac aaataaaagg agtggctaat tatgttgaat attggagaaa aagaattttg
    20161 gtttgttgta ggatcacagc atttatatgg ggaagaagca ctgcgagaag taaagaaaca
    20221 tgcacaagaa atggtcgatg aactgaatga aaatggccaa ttgccttatc caatccgcct
    20281 tcaagaatta gcagttactg ctgatacgat cacaaaaatc atgaaagagg tcaattatcg
    20341 agaagaagta gcaggtgtga tcacttggat gcatactttc tcaccagcaa aaatgtggat
    20401 ccgcgggaca aagcttctac aaaaaccatt gcttcattta gctacgcaat ataacgaaag
    20461 cattccttgg aaaacgatcg atatggattt catgaattta aatcaatccg ctcatggtga
    20521 ccgcgaatat gggttcatca atgctcgatt aaataaacaa aataaaatcg tcgttggcta
    20581 ctggaaacga cctgagatcc aaaaagagat tgctgattgg atggatgttg cagtagccta
    20641 taatgaaagt tttggaataa aagtggctcg ttttggtgac aacatgagaa acgtgggtgt
    20701 cacagaagga gacaaagtgg aagcacagat tcaatttggt tggactgttg attattttgg
    20761 tatcggagat cttgtacaag taatcgatcg cgtatcagac gaagaagtcg atcaattatt
    20821 tgaagagtac aaggaattgt ataccttcga ctatggtgat tatgaagaaa agacatggga
    20881 ggaacatgta aaagtacaag ctcagcagga aatcggtatt cgccgtttct tagaagaagg
    20941 aggctataac gcttttacga ctaattttga agacctgtat ggaatgaaac aactacctgg
    21001 actagctgta caacgtttga tggcagaagg atacggtttt gctggggaag gcgactggaa
    21061 aactgcagcg attgatcggt tgctgaaaat catggctcga gggaaagata caggattcat
    21121 ggaagattat acttacgagc tagctagtgg ccaagaagca atcttggaat cccatatgat
    21181 ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc gttgtttctc ctttatcaat
    21241 gggagataga gaagatccgg cgcgtcttgt atttgatggg aaagcaggag aaggggtagt
    21301 cgtatcgatg gcagatttcg gtacgcatta taaactgctg atcaatgaag tagaagcctt
    21361 tgaaccaacg actgaagcac ctaatttacc tgttgcacgt gtgttatgga aaactaaacc
    21421 gaatttccat gaaggtgtcc attcatggat tcaggcaggt ggcggtcatc ataccgttgt
    21481 ctcgctaaat ctaacaacag atcaaattga aacgtgggca aaattagttg agttggaaac
    21541 agtggttatt agataaaaag aggttgtgaa aaagccactt agttttaaga aataagaaga
    21601 gattgacgaa atttgctttt caactttttt gaaatttagt cttattatca atgtcagcta
    21661 acggaacacc atttatcaag atatctcacc ctcaatattt tgctctcgaa aataaggctt
    21721 atggcagtaa cttgtcatag ccttattttt atcactcagc ctactctgct ctaactgctc
    21781 tttttgcttc taggaagtca aatggtaaac agttgtctaa ttgcagaaat ttcgagatac
    21841 ttgttagtaa ggagggggtt ttatgtatca cgttcatttg attgatgcta ttccagaaca
    21901 agagatagaa aagttacaag gggaatattt caaaataata gatgatttgc cagccgaagg
    21961 aatagttatt cgtagtacga aagtgaaaga tgaatggctt acaccagatt tgctagcaat
    22021 ttcaagagct ggtgttgggg tgaacacgat caacgttgaa aaagcttctg aaaacgggac
    22081 gatcgtcatg aatacaccag gtgtaaatgc taatgcagta aaagagcttg ttttgtgctg
    22141 tttgctctta tcttcccgtc cgataatcga agcaagtcgt atggtacaaa cactaacagg
    22201 tcctaatatt ttagaacagg cagaaaacaa aagaagtgct tatgtgggac gagaattgca
    22261 aggcaaaacc atcggtcttc ttggtcttgg agcgataggc acaaaagtag cgctaagttg
    22321 ctatagctta gggatggatg tcttgggtta ttctatccgc gatgcccagt tggattatgt
    22381 tcggcaagca gatttagaaa cagttctttc tacctcggat tacatcgtag tcatgctgcc
    22441 gttgacagaa gatacaaaag gattgatcga tcaggcgaat atagaaaaaa tgaaaaaaga
    22501 tgctgtactt atcaatgtag gaagaagtga aattgttgat aaatatgctg ttatgcaagc
    22561 attggaaaaa caacatctgg ctaaatactt gactgatttt ccagaagaag agttcttaga
    22621 aaatgaccgt atacttatgt tgcctcatct aggggatcga ctcaggaagc tttggcagac
    22681 agcggacgtt tggcagttga agccttaaaa gattatcttt tgttcggtac ggttagagaa
    22741 gcggtcaatt atccatccgc acgaatgttt ttccaagcgc cgtttcgatt cactatattt
    22801 tatcaaaaga aaaccaatat attagcagag atattttctc tgctgaatga gaacgagcta
    22861 gcgatcgcag atatatcgcg taaccataag aatgagcatg tctacatatt gattgacata
    22921 gacagtactg attttgaaca gctcaatcaa gtgaaacaac agctagaaaa aatttctgga
    22981 gtacgaaagg tccgcttatt aaagaaacca gaaaggccat aaagaaagag aatatttgga
    23041 tttcattaat tcttctttcc gttctttact ttcgaatttc gtacaactaa aataagagaa
    23101 gcaacattct tttattgata gaaatgaagg agcaagcatt tgaaaagata tataagcaga
    23161 ctttcgacca aacagaaaaa gattttaaaa ggaacagcag caatcatcag tatctttttt
    23221 cttgttgttt ttagtaattt atttttgcag tggtgtcaaa atgatctatc cgtagacctt
    23281 gcgttgaaat ttgctttttc ttggcatacg gaaaaattct tcttagcctg tctcgtcttg
    23341 ctgattattc ttatttttct gattgcacta gcaggatctg tccctttagg ttcccttacg
    23401 tatgtcgtag ccatcggtgt attaggtttt gcaaattata tgaaaatgag ttatcggcaa
    23461 gaaccgattt atccagacga tttgaagatg atcactgaaa tcggattgct gaaagatatg
    23521 accggcacta tgctgtttac agtgatctta gctgccgcag gaaccgtgat aggattgttt
    23581 tgctggtaca tgtttcgaag cctgaaaaaa ggaaggcgat tccaattgat ccgattaacg
    23641 acactgcttg tggcaattgg actgctaggg tatatcagta atttcaacaa tcccgataat
    23701 ttgttgcgaa aagcatataa taaaacggca ctatggatac cttacagcca gaaaatgaac
    23761 tactataaca ctggttttat cggtgggttt ttgtacaatc tgaaggtaga accaatggat
    23821 gaacctgaag gatattcgaa agaaaagatc aaagaaatca ctgaaaaata ccagaagcta
    23881 gccgatgaaa aaaataaaac tgtagaggaa gagtcgccga acatcgtgtt cgtgatgagt
    23941 gaaagtttct cagatcctag tcgattaaac ggcgtagaag tatctggtga gccattggca
    24001 gattattatg aagtagctga tcaaacatat agtggcaata tgctttctca aaactatggc
    24061 ggtggaacag caaatatcga atttgaagca ttgactggtt tttctatggc gttatttaat
    24121 gcgcaactca ccacccctta cacgatgctt gtaccaaaaa tggatcagct tccttctatc
    24181 gtttcagcgt taaacgcaca atcataccag acgacagcga tccatccata caatacttcg
    24241 atgtataaaa gagaggatgt ttatcagaca ttaggatttg accagttcat cagcgagcgt
    24301 acgatgacct atacggatac gatagagaat aatccatata tttctgatga gtcagcttac
    24361 aaagaagtaa tgacacttct caaggaagaa aaaacacctc agttcattca tttagtcacg
    24421 atgcagacac atatgcccta cgatggaaag tatgataatc tagactatcc tgcaaaaact
    24481 tcagatggaa gtgggacact tgacttagag aattatctgc aagatatttc ctatagcagc
    24541 acggcactga agcagttcac ggaagaattg aagaatcttt ctagaaggac tttagttgtt
    24601 ttttgggggg atcatcttcc agggatctat tcagatacga tccaagcaaa aaatgacaaa
    24661 caaacattgc atgaaacaca atttcttatg ttcgacagta aaggagaatt agaaaagcaa
    24721 acgactcacg atgcaatcac cagtccgttt tattttgcag cgaatctaat ggaacagacg
    24781 aatcagacaa caaatggctt ttatcagctg cttttatcat tagaacagga acttccggcg
    24841 tttgaacgag aactgtacta tcaaaacgga cagtggtata aagaagcgca attcaatcgc
    24901 tcgcaacaag agatttacga cgaatatcaa ttgatccaat atgacattgt cgcagggaaa
    24961 caatatagtt tagcagatgg tttctttgag cacgaataag aatggcgagc tgtatagaaa
    25021 ccattaaatt ttttcaagta cttgtcattt gaggataaaa acaagtagaa taaataataa
    25081 caacaatgga gaaagtagga aaacgaacat gttaccgaat attggctatt atgtgcagtc
    25141 catcgatcaa atcgtcaagg aaacagaagc aatcggagaa ggaatgcatc cgaaatatga
    25201 agtgatcaga aacgcaattg atgaaggaaa aacaagtgaa ctggacaaat cgactcttga
    25261 agagatcatc ctgttatttg aagaaggaac aaaaaaatat cgggcaatgc ttatgaaagt
    25321 cagtactttg cgtccaccag caaaagcaat gggcattcat aaaaattttg aaaaagctta
    25381 tctttcatat gtagcaggct gtgaagaaat gatccaaagt atcgatcttg aatcaggggt
    25441 agatgaagat ctattcaata catctgaagc aaaacaagac caagcgacag accaattgac
    25501 agctgccatc caaaaaatga ctaatttgtt gttgaaaaaa taaaagaaat ctttgataac
    25561 aataaaaact agagttatct aatataaatg taatattacc tttatgatag agtgatcaaa
    25621 atcctttatg ataatgacat acctataacc aacaacaacc ttgtacttac tcaaaatcga
    25681 gtaaatactc cttttccgtc gagtctaacc atctcggcgg ttttttattt gtagaaaaga
    25741 acatcaggat agacggaaaa gtcttgtcct gatgttcttt tcagatagta tggaaagctg
    25801 attagttttc tacattctgt acatattgtt caaagtagtt ctgtaaatcg gctttgagtt
    25861 tctgatattc ctcttcgcta tactctttat cttcgatttt cccatcaaag aaaggaatat
    25921 ttagttcttc ccgtagtctg tctaatacgt cttgctgatt catttcattc tccttttctt
    25981 actaacgatc aaaataaaca cagacacatt ctggtacata gacatctttt ttcagtaatt
    26041 ccagtaatcc tgtttcagga tgacggcgat ataatgtcag attatcggtg ttctgattgg
    26101 cacacacgac aaaattattg cttggatcta gtgcgaaatc gcggggaaaa tccccttctg
    26161 ttgagatgat ttgcttgttt tgaatgtggg aaccatcttc aaaaactgcg aatacagcaa
    26221 tcgagttatg tcctcggtta gaagcataaa ggaacttgcc atcatttgaa atgcggatag
    26281 ctgcccctcc attttcaccg tcgaattctt ctggcaaggt cgatattttc tgtaattctt
    26341 caaaagcacc tgtttcttta ttataagaaa gcacgcggac tgtgctgtct aattcaccaa
    26401 ataaataagc gaattgtcca tttggatgaa agacaagatg gcgagggcct gttcctggct
    26461 ctgcagtgaa ggtagttaca agattcaatt tgccgttttc ggacacatca taagtataca
    26521 cacgatctgt accaagatca catacgacta atcgttggtc aggagtcaga tccgtatagt
    26581 ggacatgcgc atgatcttgg tttttatgag gacctgtcgc ttcttgatgg aatattgaat
    26641 ctgctgcttt cagttcgcca tctcctaata tatggtagac atttacttca cctttatgat
    26701 agttcgcccc gtatagtaat tgacgtgctt catcaaccgc tacataacac aaaggagcgc
    26761 cagactctgt taccgcattc aaaaaatgaa aatccttatc gtacgcaccg gcaccaccta
    26821 atccatccac actagtaact gtgtaaagat tgccagcttt actttttgct acataggtag
    26881 ggctattttc ttcaacagct aatgaaaggt tggctaattc ttctttttct gtatcaaggt
    26941 cgatcgtata gattccttta ctttcgcgac gcgtataggt tcctaaaatt atcttttcga
    27001 tcatatgagc tgattcctcc taattgtaat gaaatagttt tcgtttttat tctatcacat
    27061 aattattcca cacggctaaa gaatgagaat gtgttatact gtttttgaaa gcaattaaag
    27121 gagagagtgc aatgattcag gcaaagatta cagaaattgg gacagaagca ataaacaagg
    27181 aagaacccat gctgattttg ttcgataaaa cagcaacagc tgccttacga aaatattcgg
    27241 tgattcaaga agtatcaaaa gacgaacctt tttctttgaa agcaggcgga acaattgctt
    27301 ttgatgatca ggtatatacg atcgattatg tcggtccgat ggccaatggg aatttaaaga
    27361 cagtagctca cgtatcattg atttttgatg cttgtccaga agaaggacaa attgcaaatg
    27421 gaatctatct tcatccgcat agactgcctg ctattggttt gggaagccaa attttttata
    27481 actaacagga ggaattatgg aaggaaaaaa taagaagaac agaccggtat cgtggttttg
    27541 gcgctggttt ttaaataatc aagtagtcac agccttactt gttgtattgt taatattgct
    27601 gatcgtcttt ttatttacaa aagtttcata tttgtttgca ccaatctggc aatttttagc
    27661 aatcgtaggt ctgcctatca ttttagcagg aatcttgtat tacttgatga atccggtagt
    27721 tgattacttt gaaaagcgaa aagtaccaag gctttacagt atcattgggc tgtttgtttt
    27781 ggttgttgca ctaatcgcat gggggagcgt agtgatcatc cctaagattc aggaacaaac
    27841 agtgagcttc atcagtaact tccctcaata tgtagatacg attgataata aattaaccga
    27901 gattttgcgg gacccgctgt tcaaccaatt tcgcgaacag ttggaaacag caggtgataa
    27961 gttcatgaat tcggcaggcg atatgatcca agatatatct aagagtacag ttcagagtct
    28021 tggaagtttt gtcggcgcgg tagcgactat tttagtggct ctgctgacga tgccgtttat
    28081 cttattttat ctattgaaag acggtaaaca attagcacct tattttgtca aatttttacc
    28141 gacacgcatg caaaaaccga cgttgaatgt gctgagtgaa atgaatagtc aagtttcttc
    28201 ttatataaga ggacaactaa ctgtggcttt tgctgtggca atcatgttta tgattggttt
    28261 tgcggtcatt ggtttagagt atgctgtcac gttggggatt attgcgggtt ttttaaacct
    28321 gatcccttac ttaggatcat ttttagcgat gattccggct atctttttag ccattgtagc
    28381 aggtccattc atgatcatca aagtactgat cgtttttgca gtagagcaga caatagaagg
    28441 acgcttgatc tctccattag tactagggaa tcagttagca atccatccag taacgatttt
    28501 attagttctg ctgacttctg ggaaattgtt cggaatcatc ggtgtgatct tggggattcc
    28561 tgtttatgca gcagctaaag tagtgattac tcatgttttt gaatggtaca caacaatctc
    28621 ttctctttat gaagaaaatc cgttgaaaaa gcagaaagaa taacaaaaag agacagatgg
    28681 gaaaatatcc aaggcacaaa tgctgctttg aagatttttt cctctgtctc ttttgtatgc
    28741 atttatctca atttttaaaa tgatttacct aaagcagcag ctttatctaa tgcattttgc
    28801 atcaactcat ctgcacggtc agggtcataa tcgattcctt cgatgaatag ctgatctact
    28861 tgatcgatgc ctacaaagtt taaaatacct tttacatatt gagaggcaaa gtcttgtccg
    28921 ttgtatacac cgccattaga ttggatgtgc agtgcttttt tacctgtcac taatccttct
    28981 ggcccgtttt ctgtatagcg gaacgttttt ccagctacat tgacggtatc gaaccaagct
    29041 tttaattttg ttgggatatt tagattccat aaggcgtttg cgataacgat tttatcagca
    29101 gcaaggaact gatcagtcaa ttcattgaaa cgagcaattt tttgttgttg actttctgat
    29161 aaggaagtga attctgcacc agcacgtaat gcttcccaac ctgataataa ttcttcatcg
    29221 atttccggaa tatcatcgcg ataaagttcc aaaacagtca cttcatcatc tggatgattt
    29281 tctttataac ttgttaaaaa agacgtcaat gccttaactg tgcgcgattc ttctgcagtt
    29341 aggggatgac ctttgactac taaaacagat gccatatgct taccttctcc ttttattgct
    29401 atgattgtta taactatgac taacaaaatc agtttactcg aaaaagaaaa ggctatcaag
    29461 tcatattgat aaggttgaag aataaataac taaaaaaaga cagggtataa aagaggctgt
    29521 gacacgattt tttgtcacag cctcttttga atctaacaag caatattcaa gcaacactcg
    29581 actgaagagc ttccatccca ccaatgatat aaacagcacg aaaaccatta gcagtaagtt
    29641 gatcggctaa tgcttctgat cgcctgcctg tatagcttag aatataataa agtttctttg
    29701 gatctagatt ttttaattgt cttggcaaga tagcagcggg gatagaaagc gagtggctta
    29761 agtggttttt tgtgtaatct aaggtatttc ttgtatctaa aacgatgctg tcagcatcga
    29821 gaggcaattt attgaagtct tggacactaa ttgattgggc catttatctc atcctttgtt
    29881 tagtatgtgg ctattttaat gggcaactct cattaattca aatggaagga ttcctcttat
    29941 cactttttta atgtatcgga tgataatgaa aaaaactatg caaatgtctt gcaaaacagg
    30001 taggaggtgt tataatgaca tagcgtcata tgacaatgtg tcatcttgaa agaaaggaga
    30061 atccatatgc cgacacagac atttttccat ttgcctaaag aaaaacaaaa aagactaatc
    30121 gaggctgcga gaatcgagtt ttctagagta cccttaaaag aagcttcgat tgctaatatc
    30181 gtcaaattag cggatatccc tagaggcagt ttctatcagt attttgaaga taaagaggat
    30241 ctttattttt attactttga gacaatgaga cgtgacagct ctaaagatat gttacaacta
    30301 atgaaagaat ccgatggtga tctattcaaa ggattcgaat cctatttttc taaaatgatt
    30361 tatgagattt tggaaggcga gaatgcttct ttttatcgta acttgtttat gaacatggat
    30421 taccgtgcct caagaaaagt cgctcctcat acgagtcgcc aaaaaacgcc tcacgatcca
    30481 cataaagaac acaaacaaga tgcaaaagag ttgcttgaat tgatcgatcg gacgaagctg
    30541 aaagtaaaaa atgatcatga attggaatta ctgatccaat tagtgatgaa tactgtattt
    30601 acaacaattg cgcattcgta tcgcttattt tcaacatctg aagattactc agtagagcaa
    30661 gcaatcagtg aattcaattt gaaactttct tggctgaaaa acggagtata taaatagaaa
    30721 gaagggaact agatgctaaa aatcgtaaaa cggatctcct tgacttcagc aattgcagct
    30781 gcagttttca tggtgatcca agtactggca gatttatatt tgccgacact tacctccaat
    30841 attattgata aaggggtagc gcaaggagat gtcgattata tttggcatac aggttttgtg
    30901 atgatcggct tttctttgat cagtatctta gctgctatcg caaatacatt ttttgctact
    30961 cgtgaatcac agaaactggg taagcaattg cgtacggatg tctataaaaa atcagagagt
    31021 ctgacaaaag atgcgtttga taaatatgga acggcctctt tgatcacaag aacgaccaat
    31081 gatgtgactc aaatccaaat ggttacacaa atgtttttac gtatgatgat caatgcacca
    31141 atcacgttga ttggcgcgag catacttgct tatcaaaaag atcatcagtt gacaaaaatc
    31201 ttcttagttg tcatcccagt aatgattatt ttgatcggcg gaatcatgta ctttgctgtt
    31261 ccattattca aaagtatgca aaagaaaact gaccgtttaa acctggtatt tcgagaagga
    31321 ttgacagggg tcagagtgat tcgagcattc gataagacac gctttgaaga aaatcgtttt
    31381 gatttagcga ataaagatta tacaaatacg gcaatcaaag ttaatacgat cgtcgcattg
    31441 atgatgccaa tgatgacatt gatcatgagc ggtacaaacg tagcaatcac ttggtttggt
    31501 ggacactaca tagcagatat gactttggaa gtcggtaatc tgattgcatt tatgacttat
    31561 gcgatgcaga tcttgatcag ctttatgatg ctttccgcta tttttatcat ggtgcctcgt
    31621 gcacaagctt cagctgaccg tattaatgaa gtgttggatg aaaaaatcgg tatccatgac
    31681 cctgaaaatc ctaaaacagt ttcttttgct ggaaaaaacg caacattggc cttcaatcat
    31741 gtcaattatc gatatcatgg agcagaaaaa cttgcattgg aagatattga tttccaagcc
    31801 aagtcgggcg agatcgtcgc aatcataggg gggactggtt caggtaaaac gaccttagtc
    31861 aatttgatcc ctcgattata tgacatcgaa tctggttcga tccaaataaa tggaacggac
    31921 attagtgaca tgactcaata taatttgcga gaattaatgg gctttgttcc tcaaaaagca
    31981 gttctgttct ctggaacgat ccgagacaat atgcagtatg gtaagccgga tgcaacagat
    32041 gaaatgattt ggaaagcatt agaaatcgca caagctaaag attttgtttc agagatggaa
    32101 gatggcttag atagccatgt ggaacaagga ggaggaaact tctctggtgg acaaagacag
    32161 cgtctggcaa ttgcacgggc attagtcaaa acagcagata tctatgtgtt tgatgattcc
    32221 ttctcagcgt tggatttcaa aacggatgcc aatcttcgcc aagcattgaa aacgaatatg
    32281 aaagaaagta tcaccgtttt agttgctcaa cgagtaagta cagtaatgga tgcagatatg
    32341 atccttgtgc tagatgaagg aaaactggtt ggcaaaggaa cacatgaaga actattagca
    32401 acaaacgaaa cttatcaaga aatcgttcat tcacaactaa gagaggagga cttagcatga
    32461 gtaaaacaaa cacacgtccc tctcatggac cagcaggcgg tccaggacat ggtggacctg
    32521 gcagaaatct cggtgtaaaa ggtgaaaagc ctaaaaattt ctggggaacg gtacgccgat
    32581 tgtttggcta tatgtctaaa cgtatggttg cgatcatcgc tgtatttgta ctagcaatag
    32641 cagccgtgat cttccaaatc caaacaccaa aggtcttggg gaaagcaacc acagaaatat
    32701 tcaaaggtgt aatggctggc gctcaacaga tgcaacaagg ccaacaagta acaaaatttc
    32761 cgatagactt tgataaaatc gcgcagatca ttgcgacagt aatcgtgatg tatttgattt
    32821 ctgctgtatt caacttttta cagcaattta ttatgacacg tgtttcccaa cgaacagttt
    32881 atgaattacg tcatgatttg gaagccaaaa tgaatcgtgt accgatttcc tattatgata
    32941 cacatacaaa tggggatatc atgtctcgag caatcaatga tatggataac atcgcaagca
    33001 cgctgcagca aaatttgact caatttgtaa ctagtgcagt cacattgatt ggggtcatcg
    33061 tgatgatgct ttcaatcagc tggcaattga cgttagtcgc gttattgatg atccctttaa
    33121 gcttgatcat cgtgatgatc gtagcgccga aatcacaagt gtttttcgca gatcagcaaa
    33181 aaagtttagg tttattgaat aaccaagtag aagaaacata tggtggtcat accattgtta
    33241 aaacattcaa tcatactgaa aaagaccagg aagtatttga aaaagaaaat cagaatctgt
    33301 atgccgctgg ttggaaagct caatttattt cggcaatcat catgccgttg atgaatttcg
    33361 ttaaaaatct aggctatgtg ttcgtcgctg tactaggcgg agtaaaagtt gcaaatggga
    33421 acatgacatt aggggatgtt caagcgttct tgcaatatac aacacagttc tctcaaccaa
    33481 tcactcaact tgcaagtttg atgaacacta tccaatcaac agttgcttca gccgaacggg
    33541 tatttgaagt gttggatgaa gaagagatgt ccaatgaaaa atctggcctt ccagaagaaa
    33601 aagatacacc atacaaagtt cgttttgaaa atgttcaatt cggctacaca ccagataact
    33661 tgctaatgac tgattttaac ttggatgtca aacctggtga gatggtcgct attgtaggtc
    33721 cgactggtgc agggaagacg acactgatca accttcttga acggttctac gatgtttctg
    33781 gaggtagtat tcgttacgat ggtgtagata cccgtgatct ttcacgtgat gaattgcgcg
    33841 cacagttttc aatggtactg caagatactt ggctgtttac aggaagtatc tatgacaata
    33901 tcaaatatgg gaacgacgat gcaacagacg aacaaatcat tgaggctgct aaagctgctc
    33961 acgtagacga ttttgttcgt aaattgccag aaggatataa cactgtatta aacgaggatg
    34021 caagcaatat ttcgcaaggg cagcgtcagc taatcacaat tgcgcgggca ttcttagcaa
    34081 atccagatgt cctgatcctt gatgaagcaa catctagtgt agatacacga acagaaatac
    34141 tgattcaaaa agcgatgaac cgcctattgg aaaaccggac aagtttcgtt gttgctcacc
    34201 gcttgtctac aatacgcgat gcagacaaca ttattgtgat gaatcatggt tcgatcgtag
    34261 aaacaggaaa ccacgataca ttgatggaaa aagacggatt ctatgcagat ctttataaca
    34321 gtcagttttc tgaagaagaa gcttcataga atagtacaaa tttattattt tttttagggg
    34381 ctgtgacaaa agtcgtgtca cagtcccttt ttccgaataa acggtgttca atcagctgtc
    34441 cttcggtatt agctcaaaaa acggcaaaat atgagaagct atttttccgt tttgtatctc
    34501 taatactcag gtcaaaccgc tgattttaca acctcttcct taaacggtgt tctcctgcca
    34561 actcctcggt cacaagtcac aatagtcaac aaacctgaga agctgttttt tgaaattgct
    34621 ccttgtgact cggagttaaa cggcaggctc acaacctctt ggtaaacggt gttcaaaagc
    34681 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
    34741 tttcttacta ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa
    34801 agcttgcatc tgcggtaata agcccaacca aacgaaaaat aggaggagct atttttgttt
    34861 ggctgttctt attacttgat gcagaacagc tttttcacaa cctcttttta taagagtttt
    34921 tactcattaa tttacaaaaa acaagcaaaa tgcgaatttt ctcacatttt ttttaacaaa
    34981 atcaaagtta ttgcttgaaa gaagaagaat ccatcgttaa aataaagctt gacgtagagg
    35041 ggagcaagaa atatgaaaga ttttcaattg ggggctgcca cctatttaaa aagtgtagcc
    35101 attcgggtga aagaccgaga taaaatgatt gatttttaca agaacgttgt tggctttgat
    35161 ttgaaaagag aagaaaacga attagccatt ttaggaacag aagaagctgg aagtgaaatg
    35221 ttgctgctgg aagaaagtcc tagagcgaat gatcacatgg gggtgatcaa aaagctgaac
    35281 cgtttttcat tgattatccc tagcacagaa gaaatggcag atatcctttg tcgtatccgt
    35341 aaaatgaatt atccaatcaa agaagcttta gaagatcatg gacgtatggg gattttgctc
    35401 gtagacccag aagagaatga attggaaatt taccatgaag agaataagaa cgaccaaaga
    35461 gaaattccag aaacaatgga tcaagaagca ttattaagaa aatctgaaga aaaattcagt
    35521 aaactatcaa caggttctta ttttgaaaaa atccatttaa atgttactaa cttaacaaaa
    35581 gaacgccaat ttcttaatca tgtacttggt ttgaaggtac ataatgaaga agagggcatc
    35641 catgtattga atgaaggaga tttccatgtt ggtttgacag aagctagcgg aggcgcaatt
    35701 gatttgccta cagatgaagt gattggactt gattttctga aattcatgat cgatactgaa
    35761 agcatggaag gactgattgc tcacttgcag caactatcag ttgatttctt cttagacaaa
    35821 aagcacacag tactcacaat ctatgatgct ataggaatcg aatggtggtt cgtcagtgag
    35881 ccaaaagaaa agaataaaac tgacattcga tgcgacgctt aactttttct ttcttaacga
    35941 ttgtcgcatg tctcgtcatt ttatgaacgg tcatactatc gcttattcta gagatagaaa
    36001 gagaaaatag tataccgggg gaaactgcgc ggtttttcgc gcagtttttt tgtgtaaaaa
    36061 aggttgattc ctcatagatt aagctttaag cttggactgt aagaatctga tagaaaagca
    36121 ggagaaaaag atgtatccac acacgaaaca gcttattttg aaaaagatgg aagaaggatt
    36181 ttatccaggt gtagcagtcg ccttcataaa agaaaatcaa gtagaaacat ttacagccgg
    36241 ctttgcccaa gtcgttcctg aaaagcagct gatgacagaa gatctgttgt ttgatgcagc
    36301 ctcactgacg aaagtcgttg caaccacttc attagtttta cgtctattag aagatggaaa
    36361 gatagaactc gatcaaccgc ttcataaata tcttcctgaa tttgaaaatc agacaatcac
    36421 tttgcgtcat ttactgacac atacttctga tatcaaaaca tggataccaa acagagataa
    36481 gctatctcag gaagaattga tcgccgctta tttgaagcta gaaccaggtg agcaactagg
    36541 aaaagaagta aaatatacag atgccggtac gattcttctt gggttgatgc tagaggaaat
    36601 ttaccaagaa actgcagtgg aaatctttca agaacaagta ttggaaccat taggaatgat
    36661 gaacagctta tttcttcctt ttccttccaa aaaaatcgtt cccacagaac aactgccttc
    36721 gggtgaggtt ttgcgtggac aaactcatga tccgaaagca agagttttgg ctgaacatgc
    36781 aggtaacgcg ggtctcttta ttgatttgaa agacagcatt acttttgtcc aaatgtattt
    36841 gaacaaggga aaaatccgta atggtcgttt tttagaagaa gagacgattg attctttact
    36901 gcaagatcaa acaccgaaaa aaaatggcta tcgttcttta ggatgggact taaaaaagga
    36961 tgtagaagac ggtcatgcga ttctttttca tactggttat acaggtacgt ttttcttgat
    37021 tgatataaaa acacaagaag cgttgatatt tttgtcaaat cgcgtacatc ctgtcgatca
    37081 tcgtgcggag tatctcatat gtcgagatga aattgttgaa aactttttga aagagaaagc
    37141 ggtattaccg aaataatgct ataatagcta tgaaaaatga gaggagtttg aattatgcag
    37201 gaacctatgt ttttaaaacc agtttttcaa gaaaaaattt ggggaggaag tcgtttacgt
    37261 tcagtctttg gttttgatat ccctaacgat aaaatcggag aagattgggc gatcagtgcg
    37321 catcctcatg gtgtcagtgt agtagaaaat ggcgaattca aagggaaaag attagatgaa
    37381 ctatggtcag aacacaagga attgtttggc catccttcag aaccagtgtt tccattattg
    37441 atcaagatct tagacgcaga agatgatcta tctgtacaag tacatccaga tgatgcctat
    37501 gggatgaaac atgaaggtga attaggaaaa actgaatgct ggtatatcat cgatgctgaa
    37561 cctggtgcag aaatcatcta tggtcatcat gcaaagacaa gagaagaact agctgagatg
    37621 atcaaagatg ggcgctggga tgaccttttg aagaaagtac cagttaaaaa aggtgatttc
    37681 ttttatgtac caagcggaac gatccatgct attggtaaag gaatcatgat tttagaaaca
    37741 cagcaaagtt ctgacacgac ttaccgcgtg tatgactacg acagaaaaga tgcgcaagga
    37801 caaacaagag agctgcatat ccaacaatcg atcgatgtaa caactgtccc tgcgaaaaca
    37861 ccagaattac aaatcaaaga agtccgaaaa gggaattcat cgatcgtcac ttatttagaa
    37921 actgagtttt tcaatgtata tgagtgggat attaaaggaa tcacatcttt caaaaaacaa
    37981 gccccttata cattgatgac ggtaatcgaa ggagcaggag accttgtcgt tgacggaaaa
    38041 acatatccat tagaaaaagg aacaagctgt attattccaa atggcgtttc tgagtgggcg
    38101 gtccaaggtg aactagcaat cattgcgtct gaaccaggca aatagttccc agatagctga
    38161 aagcagcaga cggtttttcg tttgctgctt tttctacata aaatccgtcc atcaataaat
    38221 agaagtctgg tgaaaaagat ggaatacaaa tggaaagcaa acaacagcag tcgcgtgtct
    38281 atgaaacaac tgagaaaagc tggacatcac ttaaatgaaa tgatcatgcc aaatctaagt
    38341 ctgtttatcg cttggggaat cttgtcatta gcaacaaaag gtgtgtctgg cgatttgcac
    38401 accacattag aagaggtatg ccattggatg atccagctgc ttttacccat cttgatcagt
    38461 tattcaggcg ggaaatcggt agggggacaa aaaggggcag tcgccggtgc aattgcttct
    38521 ttagggttga tcgctcgttc caatgctcca caaatcgtag gagcgatgat tatcgggccg
    38581 cttgccggca tcgtatatga acaatttgtt tctagattcc atgtgaaatt caaggctgga
    38641 tatgaaatgc ttgtcagcaa cctactagta ggactgactg gtagcgttat ttgtataatt
    38701 ggaatcgttt ttattgaacc agttttaaat agtgttcact tgttgctcgc ctccgttgtg
    38761 tcttggctag tcacaatgaa tctgttacca cttgtcagtc tgatcttaga acctctaaaa
    38821 gtactatttt tcaataatgc aatcaatcat ggtgtattga cacctttagg aatcgaattc
    38881 tcacaaacag tcgggtactc ttccttattt ttgatggagg caaatcctgg acctggttta
    38941 gggatcttac tttctatcct ttgttttgca gaaaaacagg aaaaagtaaa tgcgagtgga
    39001 gcattgatga tccatgcgat tggcgggatt cacgaaatct attttccatt tgtcttgctg
    39061 cgaccgtatc tatttttagc tgtaatggct ggtggggcaa gcggcacgtt gatatttcaa
    39121 tggttagata cgggattagc tgcacctgta tcgcctgggt cactactcgt gatttttgcc
    39181 aatgcgcctt atccacaact agctggggta agtataggga tagcaatcag tacatttgtg
    39241 accttcattt gtgcagcgat actattgaaa ttaccaaata aagaaaaaga gaacaaaacg
    39301 gtcaaagaaa ggggagaaat cttggaacca tctatcattc attccgtcat ttttgcttgc
    39361 gactcaggta tgggatcaag tgctatggga gccgccctgc tgaaaaagaa attagcagca
    39421 gaaaaaatcg atctttctgt cggatacagc tctatctatg aattgaataa ccaagtaaat
    39481 cagctggtta tcgtgcaaca tgaactagcg ggtcttgcaa gagaaaaagt acctcaaagt
    39541 caaatctatg tactggatca ctttttggaa acagatatct tgctgtcacg aataaaagaa
    39601 gcgatccaat ttccaaagcg ggaagaaaaa aaagagatag cagatatcac agaacaaaaa
    39661 acgtcgcctg ctaatattgt atttttgtat aaaaacaaca gacgcggctc acaaactatg
    39721 ggcatgacga ttttaagtca attagcaaaa aaagcgaaaa aacagtttgt tatctcaaaa
    39781 gaatcaatag aaaaaatcaa aatccaagaa gaaacgattt atatcgcaca aagagaacta
    39841 gctgtaactg aacagctaga agaaaagatc cctcggctag ttctagttga acattgggta
    39901 gctacgaatg aatacgaaaa actcgtgaag ggagaaatat aagatgtatt tatcgcccag
    39961 agaaaaacaa ttattagcag aatttctcag tagcccgtca cctgtatcta tccaaaaaat
    40021 gatgaattta ttgaaggtca gtaaacggac tatttaccgc gaattagata atcttgaagt
    40081 ctcattgaaa tcggtaaatg cttctttgaa aaaagcagct agaggctcat ttgtcattga
    40141 ggcatcagaa gaagtattag agcaattaaa agaaaataca gatgggaaaa gtaatgactt
    40201 atccactgct aaacgacaac acgcgatttt agccgagttg ttactgacga cagagccgtt
    40261 gagtcttgct catttcatgg aatcatatcg catcagtaat accacttttt atgcagatat
    40321 caaacagttg gaagaaagta ttcgccaact gcctttggaa atcattcgga accagggata
    40381 cgagatcgaa ggaccggaaa aataccgccg gcttctgaca gcgaatgtat tagaattaga
    40441 aatcaatgaa tatgaacttt ttcatagtat ttctttcgat gcatcactga attatttttt
    40501 tcgattcgtt gatccgcaac atctatctct tgcacgcaaa gtaatcgggg aagaattgat
    40561 ccaaaaaaag accaatctaa gtgatagaaa gcttgaacat ttggttttga tgctgacatt
    40621 gacgatggag cgtgtgacga aaaatcattt actgacaaat gagacatata atagtctggc
    40681 aaacaaagaa ttactacaga cagctaaacg attgttttca cggatcgctt ctgagacaaa
    40741 acaactttat ccagtaaatg agatcgtctt ttttgcagga ttattaaatg acttcacgaa
    40801 ctcatttgat cagaatttct ttgaagagac gttcgataca cagcttgctt atttagtaaa
    40861 acaactgatt caagaagtaa gtgaagagac gaatgtccat ttttatgaag atgaaacttt
    40921 gtacaaaatg ctgttgactc atttatctgg tgtgttttca agagccgttt tacaagaaga
    40981 gacactgacc aatccgattt tagaaaaaat catggttcaa tacgaggaag ttgcgaatgc
    41041 aatccgtaaa gcagtagagc ggatttttca agaaaaaaaa ttatccgaag aagagatcgc
    41101 ttatatggtc cttcattttg cgaattcatt ggaacgaagt ccgaaagaaa gcaatatcag
    41161 tattgcagga ttttcgccaa gtggattagc ttctactagc atgttggaac tacgattgaa
    41221 aaggtatttt cccttcatcc aacaaattca tttctatcgg attgctgatt taaaaaaaat
    41281 cgatttacaa gaagaatatg atgttgttat ttctacatct attttaccag gatacggtgg
    41341 aaaatatatt ttagtctccc cgctactgct agaagatgaa gtcaagcagc taaaagaagc
    41401 tttttcgcat attgagaaga aaaaaaggca aaaaattacg tataacagca ataacgagtg
    41461 gatgatagaa gatacttatg aggaaacaat gcatttcatg gatcaaatca atcaattact
    41521 cggtcgtttt tttatccaag aactggataa tcctgagacg atttccgaaa cgattcgcag
    41581 cttatttcct tattttcctg aaggactagt aacggatgaa gaggtggttc atcgccggct
    41641 gcttaagcga tatgaacaag cacctatcgg tattcctcat acaaatatgg ggttattcca
    41701 tacatccagc tcattgattt ctaaaccgat cttttgtatt ttcaatttga aacatccgtt
    41761 gaccattgaa gggatggata gacagccagt cgatttgtcc cgtatgttag ttatgctggc
    41821 gccttcgcca atccaagaaa aagacgggat tcttttagga aagatcagtg gagcaatcat
    41881 catgaatgac ttgaacaccg agatcttccg ctcaggaaat aaagaaatcg tttatcagtt
    41941 gttagcgaaa atcctgattg aagaaataaa gaaatgaagg tgaaaagatg atacagctac
    42001 cagcagaaaa aatattgctc gatcaatcct atcaaaattg gaaagaagcg ttgaccgaga
    42061 tcggccagtg gatgacccaa caagggggaa tcaaaccggc atatatccaa tcaatgatcg
    42121 aaagacaaga aaaagcgagt gtctatatcg ggaatttcgt agccttgcca catgggactg
    42181 acaaagatga agaagtagta gaagaaggaa tctatttagt ccaagtgcct gatggtgtgg
    42241 actttggaac aaaagaggaa ccaaaaatcg cgacactctt atttgtagtg gctttaaaaa
    42301 aagaacatca gttagcggtg ctccaagatt tagccttttt ctgttcagac atcgaccaag
    42361 taatggcgct cagtgatgcg gaaaccattg aagaagtcca aatgattttt gaacatggac
    42421 atatataacc aatgttcaca gtaaaaaaag ctgccttgca tcaagtaata acttcaacca
    42481 agataaaaat aagaggagct atttttgttg ggaggttctt attactgcgg atgcaagctt
    42541 ttgaaaacta tttattctag aaaaccgttt atttgaaata ggggacggtg acaaggtttt
    42601 tgtcaccgcc cctattattt ttgtgtttta atagctcttt tttatataga tagtgcccat
    42661 acctatgaat aataaaacga tactccatat accaagtgtc aagataccat tcatgaaagt
    42721 aacctgttct gaagctagtg gcgagaaaat atctcctcct atgatcacat tttgaaaatc
    42781 aacataagaa gttggcaagt aatgactgat tgattctatc catccaatcg aagaaaacaa
    42841 gcgatactct gctaagaaaa gacaaagaag tgtacctaaa agattgacta gagaatgttc
    42901 aaataaagca gatagaagaa aactgattgt acttaaaaag agcacccaca acaacaggaa
    42961 cagaaagctc tggaaaagat actcatacat cggcaggatt cgtatctcct gaccaatgat
    43021 cgttcctaca ggataacgga agtctcccaa accatttttg attattacta gtatagagac
    43081 gatcaaaaat ggcatcacga gacttagcat agctgcacca taataaatgg taaaacggat
    43141 gctatgtttc tttaccatac tatccggtaa tagattgact agagagatta cctgctttct
    43201 tttttcataa gagatgatgg aagaaagaaa caaggcggtt atacataaaa tcaaaaaact
    43261 agaaatcatt cctgaaaaaa tattttcgta gtaatttgct aaagagagat gcgctggcaa
    43321 gtcaaaaata gaatatcttt ggatctgatg ttctttcata taacggagca actcgacacg
    43381 tttttgttgt tcaatgattg gaattccttg tctttgacca gaaatcagtc gatccagtat
    43441 atctttttca taatgataag ttgcttcgac catttgctgg acattccctg tgttttttgc
    43501 atgtaagatt gcttcgatca gtttgttgct tttctctaaa ctttccacaa attctttctc
    43561 attctcatct tctttcaacg tttcaatcaa acgttgcatc tcttcatagt tatgattcag
    43621 ttcatcgaat tctgtataag caagtttatg atcaaaaaaa agtgggaatg caaagagcac
    43681 gaccaacaat agaggaaata aaaagtttac tttgtttttt agtaagagat tgcgttcaaa
    43741 tgtaagtaaa gacatagtta cacctcatat aatttacgat aaatagctaa aatctcttcc
    43801 atatttgagg attctgtgac gatttctcca ttttttagaa aacatgcttt agaacaaatc
    43861 gattggatat tcgcgatagc gtgggaagaa agaagaatcg tcttcccctc tttctgccat
    43921 tgtctgatga ggctattcaa gatatcaata cttcctgggt ctaatccgtt caacggttca
    43981 tcgaataaca tgacagatga atcactgatc aagtaagcag caagcaagac atgttgtttc
    44041 attcctagag acatttttcc aatcggcaaa gtaaggtact cagtcatctt caatcgttgg
    44101 aaaacagttt ggcattctac tttgctctgc caatagtatt tgatcaaagt caaataatca
    44161 acagccgata gatagtcgga taactgattc gttgattcta gaaaaaagaa agaccggcga
    44221 taatctgtat aagcagtagg agagatgttg tgaagggaca attgatcata agttgttggc
    44281 agcagttggg caatgccatt cagcaaagtt gttttgccaa atccgttcgg ggcaatcaaa
    44341 gcagctactt ctcctgattc tactaaaaga ttcaattgtc gaatcacacg ttttttacca
    44401 cgtttcatgt tcatgttttt gatcagtaat gtcatagata ggcctccaat ataaaattta
    44461 ataacataat agaatggaaa acatctaaat taaatgctaa caaaagttat tgtttgcata
    44521 ttataaaaaa attaaaataa cagttttatt taaaattgaa gttttctgat accgaaatct
    44581 ttttattttg ctgacatact caaacctttg gcacaaagtt tcgtgccaaa gtacgactat
    44641 ttctcttttt agtaagcgga tacaattaga gtatcaaaag gagagaggga gagacaaaat
    44701 ggaacggacg attcccaaaa acaacatttc gctaaaagcg agggtacaaa aattaggcag
    44761 tactttatca agcatggtca tgccaaatat tggagcactg attgcctggg gtgtattaac
    44821 ggctctattt atcccagatg gttatttacc aaatgaggct ttcgctacca tggtaagccc
    44881 aatgctaact tatctgattc ctttattgat cggttatact ggcggaaaag taattgctgg
    44941 tgaccgcgga tcagttgttg gggcaattgc aacgatgggt gtgattgtcg gaacagatat
    45001 tccaatgatg ctaggcgcga tgatcatggg acctcttggt ggatatgcca tcaagaaatt
    45061 tgatcagcta ttccaaaaac ggatcaaatc cggttttgaa atgctagtca ataatttttc
    45121 agcaggtctt atcggttttg gattagcgtt attaggtttt tctgcgattg gtccagttgt
    45181 cgatgcattg actcaagcaa tggcaaaagg tgtagagatc attttatctg cccatttgat
    45241 tccattgaca agtattttta ttgaaccagc aaaaatctta tttttgaata atgcaatcaa
    45301 tcatgggatt ttgacaccat tgggaactga acaagtagca gaggctggaa aatctattct
    45361 attcttgctt gaagcaaatc ctggtccagg acttggcgta ttacttgctt ttactttgtt
    45421 tggcaaagga gcagcaaaat cttcagcacc aggagccatg atcattcact ttttaggcgg
    45481 gatccatgaa atctattttc cttacgttat gatgaaaccg ttattattct tatcagttat
    45541 aggcggagga atgtcaggaa gtttcatttt ccaattgatg gatgctggac taagagcgcc
    45601 agcttctcca gggtcgatca ttgcgattct agcgatgaca ccgttcaatt cttaccttcc
    45661 tgttattttg ggcgtgattg ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt
    45721 gaaagcggat gcgaaagata caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc
    45781 agctaagcaa gcttccaaag gtgcccaagg tattcctcaa ggagctacat tctctggcgt
    45841 acaaaaaatc atttttgctt gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat
    45901 cttgagaaaa aaagtacagg aagcagactt gcctcaaacg gtgaccaatc aagcaatcag
    45961 ccaactgaca gatgactcga atacattgat cgttactcag gtcgaactac aagaaagagc
    46021 aaaacaaaaa gcaccgagcg cgcgttttgt ctcagtagaa aactttttga attcacctcg
    46081 atatgatgaa atcattcaag aattaagcag caagcagaaa acatcagttt ctgaaattga
    46141 gaatgagcca gcagaagagt ttctttcagc aaacatcaaa caaatcaaag agattctact
    46201 tgtccacgat gatcgttccg gatcagcaac aatggggatg accgtgctca aagatatatt
    46261 agacaagaac catttaacta tcccgttaag aaaagtcaat atccaagatt taaaagatga
    46321 acctgattca ctgatcgtag ccaaagaaag tttagcggaa aaagtacaga tggctgcacc
    46381 tcaatccatt catttatcag tcaacagcat ggtcaatccg caaaaatacg aatcgattgc
    46441 acaatatttg aaacagacag cttaagggag gaaaacagca tgcaaagttt gccattcgac
    46501 atgatcgagc tcaatcgatc ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag
    46561 atgcttgtag aagcagggtg cgttgatgag gaatatatcg atgccatggt agatagagac
    46621 aaaatgttat ctgtttatat ggggaatttt atcgcgattc ctcatggaac agatgaagca
    46681 aaaaaatatg tgaaaaaatc cggcatctgt gtcgttcaag taccagatgg tgtagatttt
    46741 ggtaccgaag aagaagaaaa gattgcgact gttttgttcg gtattgcagg agtcggagat
    46801 gagcatttgc aacttgtaca gcaaatcgca ctttattgca gtgatatgga caatgttgtc
    46861 cagctagcag acgcgctcag caaagaagaa gtaacggaaa atctagcaat tgcttaagga
    46921 gagagaaaaa tgagagcagt acattttggt gcaggaaata tcggtcgagg atttattgga
    46981 gaggttttgg cagctaatgg atttgagatt actttcgtag atgtgaatca cacgatcatt
    47041 gatgctttaa atgaaagaaa agaatacgaa atcgaattag cagatgaatc aaaagaaaaa
    47101 atcactgtaa aaaatgtcaa aggaatcaac aatcaaacaa atccagaaaa agtaatccaa
    47161 gaaatcgctg aagcagattt agttactacc gccattggcc cgaatatctt accttttatt
    47221 gctgagttga ttgcaaaagg tatccaagaa agagaaaagg aaggaagcac aacatcttta
    47281 gatatcattg cctgtgaaaa tatgattggc ggttcagcat ttctagaaaa agaagtgtat
    47341 aaatatcttc cggaaacatc atttactgac aaatatatcg gctttccaaa tgcagcagtc
    47401 gatcgaatcg tcccattaca acaccatgaa gatcctttat tcgtccaagt ggaacctttc
    47461 aaagaatggg tcattgacga tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt
    47521 ttatatgtag atgatctaga accatatatc gaacgcaaac tatttagtgt caatactggt
    47581 catgcgactg tcgcttatac aggtgccttg ttaggttacc agaccattga tgaagcaatg
    47641 caagatgcgt tagttgtggc acaattaaaa tcagttttgc aggaaacagg aagtttgctg
    47701 attgccaaat gggggtttga tgcagagcag catcatgcgt atattgaaaa aatcattcat
    47761 cgcttccaaa acaaaaatat ctctgatgcg atcacacgtg tcgctcggac accattacgt
    47821 aagttaggtt atcaagaaag atttacacgt ccagtgagag aactacaaga acataacttg
    47881 acttgcccac atttgacggc aacaatgggg attattttta attactatga tcctgaagat
    47941 gaacaaagcc gtcaattgca tgaaatgaag atccatgaaa acttggaaca attgattcaa
    48001 gaagtcacag gaatcaatga tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt
    48061 tatgccaagc aagtcgctta aaataggata cgaaaaaggg gaatgtgacg aaatttttgt
    48121 cacattcccc tttttcgaat aactaggctg tttgtccttc aagatagggc agatccaatt
    48181 ctttaccgta acgttgttct aaagctaggc ggatcaaacg agctgccagg ttctcatttc
    48241 ttgcaaggat aggtccatgg aaataagaac cgaagacatt tcggtaaatg acaccttcag
    48301 attggtcttc accattattt ccttgacctt gtaccacttt ccccaaaggt cgttcacctt
    48361 cacctaagaa agtccgacca ttgtggtttt caaatccata ataagtttca ttgaattctt
    48421 cgttatggat aacgatatca ccgataaacc ggttattttc ctgacttaac gtgtagtgat
    48481 ccagtgcact gattccttgg atcttttctc cattagctcc catgtagtag tgacctagca
    48541 attggtagcc accacaaatg gctaacatga cgccatcatt ttcgatatag ttgatcaaag
    48601 aatctttctt tttctggatg tcttttgaaa tgattaattg ctcaaagtct tgaccaccgc
    48661 caaagaaaac caagtcatat ttatcagcat caaaaggttc atgtatacta acgatctccg
    48721 tatggcaaga aacccccatt ttttctgcta tatatttcaa catcaaaagg tttccgttat
    48781 caccatatgt gttcatcaaa tttccataaa ggtgggcgat attgagttca taattagcca
    48841 cggttcattc ctcctttgat aaatccttga gcagctagtt ctttacgaag ctgcaataca
    48901 gcagtgtaag tagctaaaat atagacatgc tcagttggca attctttgat tttttcgatt
    48961 acttctggta attcttttgt ttctgtcaat ttattttctg gaatcccggc aacttttaga
    49021 cgtaaagtca tatctgtatg acggtctcct ccagaaataa cagctgggat atccatatcg
    49081 gcaaagtttt caaaattacc atcccaaatc cagctgacat cgattccgtc tgcgtaattg
    49141 gcatttaata aagccactaa agaaaatgga taaggagcta gaccaatcat atcgattact
    49201 tgattgatac ctacaggatt tttgacaagt actaatgtac attttttccc attgatattg
    49261 atgacttctt gtcggccgaa tactttctcg tcatatccta aaccttgttt gattttatca
    49321 ggatcgacat tgaagtactc tgccactgca gtagctgcta aagcattgta gacattatac
    49381 ataccgccaa cttcgattcc gtattcatga ccatcgatca cgaaatctgc tgacacattg
    49441 tccatccgta ccatatcagt taatcgataa tccaattcag gacgtttgaa gtcacaattt
    49501 ggacagtaat atttccctaa gtttgcataa gtgatcattt tataatgcaa gatgtgatga
    49561 caatttggac ataagacacc gtctgtattg taatgagcca tttgttcacg atcttcttca
    49621 tggtcaaagc cataatattt tctaggattg actgtttcga tagaattgaa gattggtgaa
    49681 tcaccgttac tgataatcgt tgcatttggt gactttgcgg caccgtctac aatcatttta
    49741 taggtcgtat agatttctcc ataccgatcc atttgatcac ggaaaatatt tgtaaataag
    49801 aacagttctg gtttcatata ttctgtgact ttgctcaaac ttgcttcatc tatttctagg
    49861 acagcaaact ttttctgtcc tttttttggt tttgcctgca agaaagtcga tacaattccc
    49921 tgtaccatat tcgcaccagt agggttggtt agaacttcat cgaattgctg gcgcaaaata
    49981 ttgactgtta aagcggtagt caatgtttta ccgtttgttc cggtgacaac gacgacttgg
    50041 tagtcttttg ccaaactatc tagtatatgt ggatcgactt tcagcgctag tttacctggt
    50101 aaacttgatc cgcctttaaa aaaagttttt aatgcccatt gcgacgtttt tccaactgca
    50161 atcgcaaaat gactgcgtat tcccattatg taacctccaa aaacgatttg ctgacgaata
    50221 aattcaacta atcataccat atgttgaaaa aaaggttaaa ttgcttttct tattcttaag
    50281 aaaaggttac ggctgaaagt gcatgaaatc ggacaaaatc gcatgattta acaaaaaaac
    50341 agcttgaaac aaaatgattg tttcaagctg ctgtgatttt attattccct cttacctata
    50401 ttcccatttt aaccgataat aattttctcg cttggatatt catagcctag atctcgtgtt
    50461 tcttttggca cgaataacat caatgtatat agcattccga tccgcccaat aaacatcagt
    50521 gtagcaatcg tgattttgcc cacaacagac aagtctccgg taatccctaa agataaacca
    50581 gttgttccaa acgcagaagt tacttcgatg atgatcgaga tcagcgtttg ttcttctgta
    50641 gcagataaaa aaacgataca aaagaaacac ataccaagag aaagcataaa gacaacgact
    50701 gattttcgca catcatcttg atcgattctc cgtccaaaaa tattgatgtt atcttcactt
    50761 ttgaggaagg aatatagata caaaccgatg attgctacag tagttgttcg aatcccgccg
    50821 cctacagaac taggactgca tccgataaac atcagtaaag aaaaaatgat cagtgttgtg
    50881 atttgaaaat cacccaaatc gtgaatctgc agaccagcat tacgagtcgt aatcgaataa
    50941 aacatcgaat tgatccattt gacactcata ttggaatctt ggaataaatg gtccttttcc
    51001 aatagataaa tcaagacagt tcccgaaaca aacaagataa caaaagctaa cacagctaat
    51061 ttggtaaaca aggaaaaacg aaacggaagc ttcgcattgg aacgtttgta taatagccat
    51121 tcccggcact ccatcaatac aggaaaacca attcctccga caaaaatcaa aaacatgatc
    51181 aggatcaaga aaaaatagtc atgtgcaaag ggcttgatgg aatccccagt gacgtcaaac
    51241 ccagagttcg tgacagccga tatcgcctga taaaaaccgt aaaaaacggc atcacgccaa
    51301 cgatcaaaat agcctctgta ataaaaataa atagaaaaga atgtcccaaa aagtaattga
    51361 aaccagatta aaatagcaaa agtgatccga atcagtcgga cgataccaga caaacgcggt
    51421 tgattcatat ccgtcatgat cagctgtcgc tgtttcagtg tgatccgtcg tttagaaaaa
    51481 atgacaaaag cagtagaaat catcatgatc cccaaaccac caacttgaaa taatatttcc
    51541 agcagtataa taccgttatc attgaacaca gaattgatat caaaagtcga tagtccagtc
    51601 acactcactg tactgattgc catgaacagc atgtccacaa aagaaacatg gctatctggt
    51661 tcgcgaaaaa tcggcatata aaatagaacg agtgataaga ccgtcatcaa tatataataa
    51721 aaaacgatga tttggatcga tgagaaatga ttggcagcaa acttctgtcc tcgccgctgc
    51781 agccttcgaa agaataaatc aacatgcaca gtactatcct cctgacaata gtcgtttcta
    51841 ccagtataca aggggaatta ggaatactca aagaaattat caaacagata acaatacagc
    51901 tttcgattaa aatagaaatg gcttataata aggaaaatcc tagaaaaaag aaggaggagg
    51961 aataacaaat tgattaatgc tcaacaaatc gtagaaaaaa tgaaaaatca aaaaataaat
    52021 tatgatacag ttctgaaaaa aatgatcatg caatgggaga aagaagaagt aaagcctaag
    52081 atattaatac atacatgctg tgctccttgc agtacgcaca cattggaatt catgtctcaa
    52141 tatgcagaga tcacgttatt tttttcaaat tcgaatattc atcctaaaag tgagtatttg
    52201 cgaagattac atgaacaaaa acgatttgta gaccaattta atgagaaaac tgaccatcat
    52261 gttgcgctaa tcgttgatga ataccgtcca aatgaattca ccaaaatggt tttaacaaac
    52321 catcttgaaa acgaaaaaga aggtggtgct cgctgcagtg catgcttcaa tatgcgatta
    52381 gatcttgttg cgcaaaaagc gcaagaacta ggatacgatt actttggaag tgccctgacc
    52441 atctcgccaa agaaaaatgc agctttgatc aatcagatcg gaatggatat ccaaaagatt
    52501 tacgggacac aatacttgcc aagtgatttc aagaaaaaca aaggctatga acgctctttg
    52561 caaatgtgca aagagtatga tatttatcgc caatgttatt gtggttgcgt gttcgctgcc
    52621 aagcagcaag gatgcgatct gaaagaaatc aatcgggaag caaaagctta cttaaaaaca
    52681 tcaaccgtag tttttgagga aaattaaaga aatctttcgg aaaaacgtaa gaaaaaaata
    52741 aagaatttat ggtagaaata aggtagactg tataatttgt ccgtagtaaa gtaaggaaac
    52801 aagaataaat ttcaatgaag gaagagataa gatgcgaaaa gtagcaccta ttttgaagcc
    52861 aatcgctgta ttaacaatct cgacgattgt cgcgactact acaggtattg tgaatagttc
    52921 cagctaccgt gcaccaagtt caagttctag tactgtagaa gaaacaacac caactacttc
    52981 agtctcgaca tctgaaccaa caacaacgga aagtttgaat gaaaccacta cgacaagtac
    53041 atgggacgat acaacaacta gttcgtcgta tgatgagaca tatacaactg acagctcaag
    53101 cgaaacaagt aatacggtac cagatggcac tccatcggat tcaaccaatc caagtacaag
    53161 tccggataca agtgctagct ccgaaacgat accaagcagt acgactgtag atgataacac
    53221 gcaaaatgat gatgcagcac gtacagcaca gcaaaaccaa gattcaggaa atacccaaac
    53281 gtataaccag caagcaggtg attctcaatg acatttttac tagaccttat caacggggtg
    53341 cataaattcc ttggctattt agacatcagt cctaaatatc ttaaccgtgg atatacgatt
    53401 ttaagtgtga ttcccacgct ttatttattg agaattgttt atggactttg gcaaaaccaa
    53461 aactatcttc aatttttctt atatggactt gcatttctag tgcttgttta ttttactgtt
    53521 ttaaatgtat tttattactt tttagataaa aacacgaaag ccgatgtcac tcagcttttt
    53581 gtgaagtatt tacctgatga agcgttcaac atccagacag aagaatcgat agataatgga
    53641 aattcgatcg ataaagtgaa tacacaagag gtcatggttt cttatgacga agattatcaa
    53701 ttgaaattag ccgagaatat gcggtatttg attggaaatg gtgagatcaa aacaaatgaa
    53761 ctcggtcgca tggatggatt tctggtcgat agaaatacat tgtatcctta ttactatgtc
    53821 aagaaaacag gagagaaaga gtatgttcta aatattggac gaagctactc ggacatggaa
    53881 aaagtaggga ccattcgact tgactcaaca gaagacacat tggaacctgt cggattgttc
    53941 atcgtcggcg gtgattttgt caaagacggg ttacgctatc atgagcctta tcgtctgaaa
    54001 ttgattgcaa aaaaggcaca gtcgcaggaa gaactagtgg tttatagtcg tagccaaaga
    54061 agaagacatt cataatgtga tgaaacgccg tgtatcagtg gttgcgataa aaacgtttat
    54121 tcggaataag ggggatgtga cacgtctttt gtcacgttcc ttttttatat aacgagaaag
    54181 ataagacgaa cacatcactt attctttcct ttcatttata aacattgtag aataaagaaa
    54241 gtacaacgaa gtattgtgaa aaaacgtaat aaataacgag gaaaatacaa gtaggaggta
    54301 agctgtgaaa gaatttttga aaaattgggg gattttgatc cttgtattag cggctatttt
    54361 attagctaga gtgtacgtgt ttacaccagt tactgtaaac gggcattcga tggaccctac
    54421 attaagtgat ggacaacgat tgatttcatc taagatctca aactatgaac gaatggatat
    54481 tattactaca aaggaaccgg gggatgaaga acggatgatt gttaaacgga tcatcggtat
    54541 gcctggagat acagtcaaga tggaaaatga ccagttgacc attaatggca aaaagtatga
    54601 tgaaccttat ctggatgagt tcaaaaaaga attttcagaa gataagttac aaggagaata
    54661 tgcttatagc tcaggattcc aagcacaagc agaaagttca tccaccttta cgagtgactt
    54721 tgaatatact gtgccaaaag gaaagtatct tgttctaggc gataaccgct tgatttcaaa
    54781 agacagccga atgtttgggt tagtcgacaa agatatgatc caaggaaaag ttgttttccg
    54841 ttattggcct ctttcagaaa ttaaaatcat gtgatgaaaa aataaaaaga agctgtgaca
    54901 tgttttttgt cacagcttct ttttccgaaa attccttctt atttcttgga gttgctcact
    54961 ttttttgcaa tctcatttta agctttttgg ctgatggata agaaaagcga agcgtttttg
    55021 ttcataaata gcttttcccc acgatggaag gtatgactag tagcagagac aaagcccgtt
    55081 ttttccaatc gattattcaa ttcttcttgg ctaaagccat tatggacttt cggatgacta
    55141 atttgttcat tcttgtcaaa gtctacgatg atcaattgac cgccaggagc taaaatttca
    55201 taaagttttg taagaatatt ctccgtatca ggaatgtgta aaagtacaag tgatagaagg
    55261 atgagattag cttggacagc aggaaattcg acagaagcat cagcgtggat cgttcggaca
    55321 tttttcagat ctgctgcttg gatcttttct tctgccattt tcagcatagt ttctgaagca
    55381 tcggcaatga tcagttcttt gaatctttcc gcaagcggta aactaacaag cccagttccg
    55441 cctccgtaat ccagcaggac tttttggtgg ctgtcgcgcg gtagataagt agttagttct
    55501 tcgcgaataa tcttggctaa tgcttgacgg tcaggggaat cataatggtt agcgatggaa
    55561 tcaaaaatat tttttgtcat caaatagctc cttacgtaaa actttgaaat tttaacttat
    55621 tatagcacgt gaaaagaaag aaagcatgct atactcaatt caaaaaggga gaatctagaa
    55681 agaatatgcc gatatcgttt gtaaaagata gagaggaaaa aggaaagtgt gtccgagaaa
    55741 tattactaga cttgcctgag tggtttgggc ttcctgaatc aacggaaaaa tatatcgagg
    55801 aatcaagtaa actaccttta tggtgtgaaa aaagaaaaga agaatattta ggctttatca
    55861 ctctttcaca gacaagtgaa gatactgccg aaatctattc tattgtatgg gaataaaaaa
    55921 agactatcat caccaaggat taggaacaaa actttttaaa gaagcagaag gttacgcagc
    55981 aaaacattac aaatatttgc aagtgaagac tgtagacgag gggcactact ctatatatga
    56041 tcaaactatt tgtttctacg aatcattagg attttcaagg ttggaagttt ttccaaattt
    56101 atgggatgaa tggaacccat gtttagttct tgtcaaaaaa ttggaacaaa agtgagcaat
    56161 agatttaata ctaatctgtg cttgatgaaa attctcactg taaaaaatgc tgaaaatcaa
    56221 gtataatacc ataggaacaa actggaaggt gaaaaagagc atggcacaac tattttttaa
    56281 gtatggcgca atgaatagcg gcaagacgat cgagatccta aaagtcgcac ataattatga
    56341 agaacaaaat aagccagttg tcttaatgac aagcggtatt gatacaagag atggtttagg
    56401 cgttgtttca agccgaatcg gactgaaacg taatgcgatg ccgatatttg aagaaacaga
    56461 cgtatttgac gtaattcaga aaatggaaag caaaccctat tgtgtgctga tcgatgaagc
    56521 gcaattctta aaaaagaaac atgtcattgc ttttacgaga attgtggacg aattggatat
    56581 tccggttatg gcatttggtt tgaaaaatga ttttcgaaac gagttatttg aaggatcaaa
    56641 atatttattg ctttatgcag acaaaatcga agaaatgaaa acaatctgtt ggttctgcca
    56701 caaaaaagca atcatgaatt tacattatat tgatggaaac cctgtttatg aaggcgatca
    56761 agtgcaaatt ggcggaaatg aagcttatta ccccgtttgt cgaaaacatt actttcaccc
    56821 atcaatgatt acagaaggag aataagcatg tacgatcaat tacaagcaat tgaagaccgt
    56881 tatgaagagc ttggagaact tttaagtgac ccagaagtga tttctgatac aaagcgtttt
    56941 atgcagcttt caaaagaaga agcaaacacg cgcgaaacag tcgaagttta tcgtcgttat
    57001 aaagaagtag tacaaggaat caccgataca gaagaactgc ttggtgaaaa actggatgat
    57061 gaaatggctg aactagcaaa agaagaatta tctgaattaa aaaaagaaaa agaagtctta
    57121 gaagaaaaaa tcaagatctt gcttttacca aaagatccaa atgacgataa aaatatcatc
    57181 atggaaatcc gtggagcagc aggcggagac gaagcagcat tattcgctgg cgatcttttc
    57241 aatatgtacc aaaagtatgc tgaagcacaa ggctggaaaa cagaagtgat ggaagctaac
    57301 gttaccggaa tcggtggata taaagaagtg atcatgatga tcaccggaga caacgtatat
    57361 tcgaaattaa aatatgaaag tggtgcgcac cgcgtccagc gtgttccttc aactgaatcg
    57421 caagggcgta tccatacttc caccgcaaca gttgtcgtga tgccagaagc tgaagaagta
    57481 gagatcgata ttgaagacaa agatatccgt acagatatct atcatgcttc aggtgctggc
    57541 ggacagcacg tcaataaaac agcatctgct gtacgtttga ctcacttacc aacagggatc
    57601 gttgttgcta tgcaagatga acgttctcaa atcaagaacc gtgaaaaagc aatgaaaatc
    57661 ttgcgtgctc gtgtgtacga taagatccag caagaagcac aaagcgagta tgatgccaat
    57721 cgaaaatcag cagtcggaac tggggatcgt tcagaacgga tccggacgta taatttcccg
    57781 caaaatcgtg tgactgatca tcgtatcgga ttaactatcc aaaaattaga tcagatttta
    57841 gcagggaaat tagatgagat cgttgatgca ttgatcatgt atgatcaaac atcaaaactg
    57901 gaagctatgc aaaatgggta aaatgaccta tcgagaagtc ctgtcacggg cttcttcttt
    57961 tttagaggat catgggaaag aagcatatag tatccaattt ttatttttgg aacgaaaaca
    58021 atggaaaaaa cttgattggc tccttcatat gaacgaagag atttcagaag aagaacagcg
    58081 gttgatcgaa accgatctgc gacttctgct tgcagatcat ccacctcagt atttgctagg
    58141 atatgcagat ttttatgatc atcggttaaa ggtaactgaa gcaacactga tccctcgacc
    58201 agaaacagag gagttagtcg aatggtgttt agacgagacg cctggtgtac cgttagaagt
    58261 gatagatatt gggacaggga ctggagcaat tgctattagt ttgaaagcag caagaaaaaa
    58321 ttggcatata tctgctgtgg atctttcaga agaagcatta gaagtagcga aagaaaatgc
    58381 tcagaaagaa ggaacgaaaa tcagttttta tcatggagat acattaggac cagtaatgga
    58441 tcagcaattt gatgtgatta tttctaatcc gccgtacatc agtcggaatg aatgggaact
    58501 gatggatgaa agtgtccggt cattcgaacc taaaatggcc ctttttgctg agaacgatgg
    58561 attagctatt tatgaaaaaa tagcagttga agcaccaagc gtactaaaat caaatggaaa
    58621 gattttttta gaaatcggat ttcgccaagg tgaagcagta aaaaaaatat ttcagcaagc
    58681 atttccagat aaaaaagtgg cgataaaaaa ggatcttttt ggcaatgaac gaatgatcca
    58741 agtttatggt tgaagttgtg gatgaaaaat tttttatttg tttttttatt ataaaaaaac
    58801 acttatttga caaggatgta aacgactttt tggtttatca acaagtttat ccactattga
    58861 caaatagatt atccacaaac agtggaaaac ttgaggataa actcagacaa aataagaatg
    58921 tatgaaaaag gggttgtgga taaactgtgg aaacgaaacg atatgatgaa acagaattgc
    58981 aagaggcagc cgaagcactg aaagaaggag aacttgttgc ttttcctacc gaaacagtat
    59041 acggattggg cgccaacgct cttttaccaa acgctgtcaa gaaagtgttt tctgtaaaag
    59101 ggagaccgca agataatcca ttaatcgtcc atgtcgcctc ttttgaacaa gtaaaagagt
    59161 atgtggacaa ctttcatccg gcagcagaga agattgtgaa gaacttctgg cctggaccat
    59221 taactttgat atttaagatc aaaaaagaca ctttgccctc agttgtgacg ggaggattat
    59281 ccacagctgc atttcgtata ccggataaca aaaaaacact agaagtaatc gaactttccg
    59341 gtgttccgct tgtcggtcca agtgcaaata cgtcgggaaa accaagtcca acgactgcag
    59401 atcatgtata tcatgatcta caaggaaaaa ttaccgggat tattgatgat ggagctacgc
    59461 gaattggtgt agaatccact gttcttgatt taagtgatcc aacagccatg cctatgattt
    59521 tacgaccagg tgcagtaacg aaagaacaaa tcgaagcagt catcgaatct cctgttgcta
    59581 ttgatcagca tcttgtaaaa gaaaatgaaa caccaaaagc ccctgggatg aaatacaaac
    59641 actactcacc cgatacacgt gttctgatgg tcagagatgg cgattggtca actgcagttc
    59701 aatgggcaaa aaataagaag atccgtgcag gtgtcatagc aagccccgaa attgcggatc
    59761 aagtgcggac agatactgca gctgtttata tgtataacga caactcagta gaagcggcgg
    59821 ctaaaggatt gtttgcaggt ctaagaggat tagatgaacc aacacttgga ttagatttga
    59881 tttttgtaca agtgtatcca gaaactggat tggggaatgc ttacatgaat cgcttgaaaa
    59941 aagcagcagg acaaaattat tttgaaaaat gatcaattgg ttattgtcgc attcttttag
    60001 tatgatacaa tgctatcaag taaatcttct atcagtgaac gaaaaactaa gcgtaactaa
    60061 tcgtaataga agagatacga atatttggca atttgttttt gatgggaaaa ataaaattgt
    60121 aggaaaataa aaaggagctg ttgtttgtgg tagattacaa aacgtttgac cctgatcttt
    60181 gggctgcgat cgccaaagaa gaagaaagac aagaacataa tttggagctg attgcctctg
    60241 aaaactttgt ctccgaagca gtgatggctg cacaaggaag tatcttgaca aataaatacg
    60301 cagaaggtta tccagggcat cgctattatg gtgggtgtga atttgtcgat atcgttgaaa
    60361 atttagcgat tgaccgtgca aaagaattat tcggagcgaa atttgcgaac gtccagcctc
    60421 actccggctc tcaagccaac acggctgctt atctcgcttt ggtagaacca ggggacacga
    60481 tcttaggaat ggatctttcc gcaggcggac atctaacaca tggatcacca gtcaacttca
    60541 gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc gacaacagaa gttattgatt
    60601 ataatgtggt acgtatttta gctagaaaac atcaaccaaa attgatcgta gcaggtgcca
    60661 gtgcttacgg acgaacgatc gactttgcga aattcagaga aatcgcagat gaagtaggcg
    60721 caaaactgat ggtagatatg gcacatatcg caggattagt agcagcagga cttcatccta
    60781 atccagttcc ttatgcagat atcacaacga ctaccactca taaaacattg cgtggccctc
    60841 gaggcggcat gattttgaca aacgatgaag cattagcaaa aaaaataaat agtgcggtat
    60901 ttcctggtat tcaaggcggc cctctagagc atgtgattgc gggtaaagca gtagcattca
    60961 aagaagcatt agatcctgca tttaaagaat atagtgaaca aatcatcgcc aatgcaaaag
    61021 caatggtcaa agttttcaat caagcaattg gaaccagagt catttcaggg gcgacagata
    61081 atcatctgat gttgatcgat gttcgcgagt taggtataaa tggaaaagaa gcagagagca
    61141 ttttggacag tgtgaatatc actgtaaata aaaactcgat tccttttgaa acactaagtc
    61201 cattcaaaac aagcggtatt cgaatcggca cgccagcaat cactactcgc gggttcaaag
    61261 aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc attacaagca aaagatgata
    61321 acgctcaact agacgaagtc aaaacaggtg tacgcgaatt aactgaaaaa tttccgttgc
    61381 ataaaaaata aaaatttaaa ttccattagt aaacagaaaa gttcaatgtt gggcttttct
    61441 gtttattttt tgggaaaacg gattctttga caatatcggt gatgctctct agtatatgag
    61501 gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa gtcatgactg atcgtatctc
    61561 gttagaaaag gaagcagtag cattcagtga tgcaaatgcg cctcatccgc gtatctatga
    61621 actagcaccg gaagaaggaa gagttttact agaagaagta caagaatcac ctgtgaacaa
    61681 atattccgtc gatattgaag acttgatggt ggatactggc gaatggggaa aactcaatat
    61741 tcgtctcgta cgaccagaag ggaatatgga taaattacca gttatttttt atattcatgg
    61801 agcaggctgg gtttttggca gtgctcaaac ccatgataaa ttgatcagag aacttgcggt
    61861 tcgtacgaat tccgtcgttg tctttccaga atatagtcga tcaccagaag caaaatatcc
    61921 aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg aataaattag ccaagtcaaa
    61981 aaatctggat ctcgagaatc taatcgttgc aggagattca gtaggtggta acatggctac
    62041 agtcatgacg atcttaacaa aagaacgaaa gggcctccca atatccaagc aattgctgta
    62101 ttatccagtc acaaacgctg attttgatac agattcctat catgaattcg gagaaaacta
    62161 ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa tatacgacga atgaagaaga
    62221 gcgtgctgaa atcacagctt cgcctttgag agcaaatata aatgaactaa atgatcttcc
    62281 ttcagcaatg attttaacgg gagaagcgga tgtattaaga gatgaaggtg aggcttatgc
    62341 gcgcaaactt cgtgatgcag gggttcctgt tacacaagta cgcttccaag gaatcatcca
    62401 tgattttgtc atggtaaatg ctatggatca gacaaaggca acgcgtgcag caatggcatt
    62461 atctacttct tggatcaaga aaaaagaata agctttgctt gtccgcagga aaacttcttt
    62521 tcaaaaacgg attttcttag gaaaaatggc gagtttgact agtcaatcac ttgattttgt
    62581 tttacaatta aaaagaaacg acagaacaaa ggagagctta aaaaatgggc aaatttcaag
    62641 tgatcgatca tccattgatt caacacaaac taactattat tcgggacaaa aactgcggaa
    62701 caaaggtttt tcgtgaagta gtcgatgaaa tcgctatgtt gatggcatat gaagtatcac
    62761 gggatatgcc attggaagac atcgtgatcg aaacaccgat cactgaaaca acacaaaaaa
    62821 cgttgtctgg taaaaaagta gcgattattc caatcttgcg cgcaggtatt ggaatggttg
    62881 acggtatctt ggaattgatt ccagctgcta aagtgggaca tatcggtctt tatcgagatg
    62941 aagaaacatt ggaaccacat gaatatttcg tcaaacttcc agaagacatt gatgcacgtc
    63001 agttgtttgt agtagatcca atgttggcaa caggcggatc agcaatcatg gcaatcgatg
    63061 cattgaaaga acgtggcgcg tcgaacatta aatttgtttg tttggtagca gcacctgagg
    63121 gagtaaaagc tttgcaagaa gcgcatcctg atattgacat ctatacagct tctctagatg
    63181 aaaaattgaa tgaacatggt tatatcgttc ctggactagg ggatgcgggt gaccgtttgt
    63241 ttggtacaaa ataagtaaat acgaaaaagg gtgtgacaca acttttgtca catccttttt
    63301 tggtcatttt taacgtttga tgcagaacag ctttttcaca gctttcgttt gcatataaaa
    63361 aaaccatccc aaaaatgcag agatggtcaa attcaaatct tttttagttc tttgtagtta
    63421 acgattacta cacccttact ttgcgtcatc aagttcgcat tttctgcagt ttcatcaatc
    63481 tcaacaacag cggaattttt caattgcttt gaaatcgttc cagtttgcga cttgccgtga
    63541 attgtgaaca ttacgtgagt attgatcttg aatttttcac caatttccga tggtacgata
    63601 gttgtatcaa attttccaaa agttgccatt ttcataaaac ctgcctttca catcaattat
    63661 accacgtttt atgaaaaata tcatgaaatt aaactaattt tccatcggac tctttacaat
    63721 cagccaaata gagtatataa tttcttttgg ataaggccag cttagctcag ttggtagagc
    63781 aacgcactcg taacgcgtag gtcgtaggtt cgatccctat agctggcatt aaaagagaag
    63841 acagatatat caaggatttt taatccattg atacatctgt ctttttgttt aaaggaaata
    63901 aagtatgctt tcttagcaat agagcatgta ttatttctat aaaaatttag gagaataaaa
    63961 cgactagcaa tatagctgtg aaatcaaata tcacatgaac tgcccaactt gtaatgatcg
    64021 agttgctttt aatggcagct tggttgaata acaatcttgc caccccttgt atcaaaagga
    64081 tgtgtgctac agtgtgtaaa atatttccat tatcataagt actgtaatgg ataaatccaa
    64141 agatgattgc tgacaagaaa ctagcgacag gaatgctgaa cttactagaa aaaatagcga
    64201 gaaaatacac tgagaacaat tcttctccaa gtatcatgat aggcaaaata ataaataata
    64261 acgggctgaa agcctcattt attggattgc ctgttaaacc ccatttcaaa atttgttgca
    64321 aaaataccga gactgcggca ctgattacta cattgataaa aaaatatttg gctatatttt
    64381 tgacaggagc gacaggtttc gagaacattt ttttcgtatc ttttttacca aaaacaagtg
    64441 taaatgctat aaatccagtg atagttaaag aaaaaatgct aataatggat ggcgcaaata
    64501 gcaaccctaa caaaccgagt acaacgacaa tagtgcccaa tatccctttt ttaagttcta
    64561 ttttttttcc taaaatgtta ttcatgataa taactcctac tattcagttt ttgaagatag
    64621 agtcagtata catgaagagt taaaataggc aatgtttttt tatggatatt tgtattgcta
    64681 aaaatttatc ttatatattc attttgatta tactactttt atttgtaaaa attttctagc
    64741 aaaaccaagt gcttgcaact gattaagcgt acaatttttt agatgttccg agcgtacttc
    64801 aatcatttca aaggagttgc tacttaaatc gatatttttt aaatcagtat caaaaaagct
    64861 agcattttct aaatcattag tagaaaacat tattttagtg agggaacagt ctacaaaatc
    64921 tgcgcttgat aagacagagt gctcaaaggt actttctttt atgtttgaac cagataaatt
    64981 tacatagtcc attttacaat tctcgaataa cgtgttccca atataacttt ccatcaattt
    65041 agtacctgtt aatttacaat tgacaaattt cacgcgatgc aacatgcttt ttgtcaatat
    65101 acagcctgaa aaatcacagt tttcgaatat acagtcggta atctccattc tttgaaaatc
    65161 attggaagca aaaaatgcat acttgaaaat agatttgtaa ataattggat ggcagattat
    65221 ttggttttcg atgacctctt tttgtgaaag gatattatac agttcaaaat cttcgttttc
    65281 atagatatga aaaaaggatc ctttattcaa gcttgcttca ttaactttag gaaaaatcat
    65341 atttaattac ctccattttc ataaagtcat attaacaaaa gctaaaccaa cttacattag
    65401 cagaaaatga gataggtttt cgcctttttc gataacgata gtgtcgctat ctcttgaaac
    65461 gttcttagaa ttgccctgag agacacaaat taggcgtaat tctaataaaa atcttagaat
    65521 aaaaattttt actgatttaa tttagaatgt ttttaaaata aaaatcgttg ccataacagt
    65581 gtattgaaag gttttaacaa tccagtaatg atttaaaaaa atctagatgt agcgtatact
    65641 ttgtttatag aatgtagtgg gaagaaatat atttttggga ggaaatcgtg atgtctaaaa
    65701 gtgaagaaat agaaaacatg gtactaaaat tatatgatct cgttttaaat ccagagatca
    65761 aagaaaagga acgtatactt ttgatagacg caaagacagg cttagaaaaa ggtcagtatt
    65821 atcctaaagt cataaataat ttggaacgta gtttacgtcc gttagcaatc aggggagaat
    65881 tgtctaaacc agtatcaccg ttttatatgg agatatcaac aataggaaaa tttgaaaaag
    65941 aattaggtcg aggaatggct tcggcaccga ttacttttgg gcatttataa aaacgagtta
    66001 ttcatctcaa tgaattaaaa agtatagtca atctcataat gcaggctagg ttcatttatt
    66061 tgaacagagt ctgtattttt ttattctaaa taaagaaaca gatgtttctt tatattgtat
    66121 tttatccttt aattaacaca aaaacagcta gtataaacta ataatgttaa ttaaaagtgt
    66181 attttatgaa actatcgtta tttattaatg ttgcattttt gtaagttaaa aattggaaat
    66241 atagattgaa aaaatgaaag caataagtaa aataaaatga atatatgcga aaaaataatt
    66301 tacattagga aaaaccttgt aaaaacagtt ttttggcata tttatttttt ataaatattt
    66361 gtaaaataac tatcaatttt ttagttaaaa gattggaaat gagtggggga ggaattgaag
    66421 aaaggatttt tgagagatga tatagtaggt tttattttta gttaaaatag gggagataga
    66481 aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta ctaatataac
    66541 atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac
    66601 ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc
    66661 gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag
    66721 tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa
    66781 aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat
    66841 taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac
    66901 cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac
    66961 tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg
    67021 agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg
    67081 cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga
    67141 tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga
    67201 agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac
    67261 tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt
    67321 gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc
    67381 ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa
    67441 aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa
    67501 atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga
    67561 tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc
    67621 tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg
    67681 gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac
    67741 agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa
    67801 aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag
    67861 tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac
    67921 ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa
    67981 agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga
    68041 aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac
    68101 atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt
    68161 agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg
    68221 tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga
    68281 caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc
    68341 ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga
    68401 gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca
    68461 tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca
    68521 gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc
    68581 tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac aaaaatataa
    68641 aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa aagaataaaa
    68701 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
    68761 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
    68821 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
    68881 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
    68941 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
    69001 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
    69061 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
    69121 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
    69181 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
    69241 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
    69301 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
    69361 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
    69421 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
    69481 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
    69541 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
    69601 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
    69661 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
    69721 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
    69781 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
    69841 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
    69901 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
    69961 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
    70021 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
    70081 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
    70141 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
    70201 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
    70261 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
    70321 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
    70381 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
    70441 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcgtaa tgaaacaagt
    70501 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
    70561 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
    70621 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
    70681 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
    70741 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
    70801 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
    70861 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
    70921 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
    70981 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
    71041 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
    71101 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
    71161 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
    71221 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
    71281 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
    71341 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
    71401 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
    71461 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
    71521 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
    71581 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
    71641 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
    71701 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
    71761 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
    71821 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
    71881 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
    71941 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
    72001 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
    72061 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
    72121 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
    72181 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
    72241 aacctccttt acatattttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
    72301 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
    72361 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
    72421 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
    72481 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
    72541 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
    72601 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
    72661 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
    72721 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
    72781 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
    72841 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
    72901 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
    72961 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
    73021 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
    73081 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
    73141 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
    73201 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
    73261 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
    73321 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
    73381 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
    73441 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
    73501 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
    73561 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
    73621 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
    73681 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
    73741 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
    73801 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
    73861 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
    73921 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
    73981 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
    74041 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
    74101 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
    74161 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
    74221 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
    74281 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
    74341 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
    74401 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
    74461 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
    74521 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
    74581 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
    74641 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
    74701 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
    74761 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
    74821 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
    74881 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
    74941 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
    75001 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
    75061 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
    75121 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
    75181 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
    75241 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
    75301 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
    75361 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
    75421 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
    75481 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
    75541 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
    75601 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
    75661 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
    75721 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
    75781 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
    75841 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
    75901 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
    75961 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
    76021 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
    76081 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
    76141 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
    76201 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
    76261 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
    76321 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
    76381 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
    76441 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
    76501 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
    76561 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
    76621 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
    76681 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
    76741 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
    76801 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
    76861 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
    76921 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
    76981 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
    77041 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
    77101 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
    77161 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
    77221 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
    77281 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
    77341 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
    77401 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
    77461 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
    77521 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
    77581 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
    77641 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
    77701 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
    77761 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
    77821 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
    77881 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
    77941 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
    78001 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
    78061 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
    78121 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
    78181 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
    78241 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
    78301 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
    78361 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
    78421 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
    78481 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
    78541 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
    78601 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
    78661 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    78721 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    78781 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    78841 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    78901 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    78961 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    79021 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    79081 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    79141 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    79201 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    79261 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    79321 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    79381 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    79441 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    79501 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    79561 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    79621 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    79681 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    79741 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    79801 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    79861 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    79921 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    79981 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    80041 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    80101 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    80161 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    80221 gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    80281 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    80341 cgtttattca gataagggac tgtgacaaga cttttgtcac agtttctttt tgtttattct
    80401 tttttatctt tgtatggcaa taatagacaa tataacgaaa attatttaat ggcttttgga
    80461 agaccatgga aaaccttgta caaagctatt ttttctgaaa aattccttga aacctcacga
    80521 aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg attttttaat aattattcat
    80581 taaactctta aacaacttca gaaaatatta attattctga aaattccaat aaaggggtac
    80641 gtaaaatgaa aaagaaattt acatttggtt tggttgcggt atgcggcctt gtacttgctg
    80701 gatgttatgg cggcagttca gccacaagca gtagttcagc aaaaagcggt tcagcagatg
    80761 gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg
    80821 caacagatac aatcagtttt acagcattga ataacgttta tgaaggaatt taccgtcttg
    80881 atgaagacag caaaccacag ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg
    80941 gattaactta caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta
    81001 cagcagcgga ttacgtttat ggctggcaac gaacagtaga cccagctaca gcttctgaat
    81061 atgcttacct gtatgcgcca gttgaaaatg cagaagacat tacagctgga aagaaagata
    81121 aatcagaact agggatcaaa gcagttggtg actatgaatt agaaatcaaa ttgacgaaac
    81181 aaacaccata tttccaatat ctgcttgctt tcccatcatt tttcccacaa aaacaatccg
    81241 tagtggaaga aaatggggat gcctatgcat ctgcaagtga taaagctgtt tataatggac
    81301 cattcacatt agaagatttt gatggaccag gaacagatac agaatggact tataagaaaa
    81361 atgatcatta ctgggacaaa gatacagtaa aactttctga aatcaaagtc agtgtagtaa
    81421 aagaatcttc tactgcattg aacttattca aagatggcca agcagatgac gtgatcttgt
    81481 ctggtgagtt agcacagcaa aacgcaaacg atccagctta tacttctgtt aaagaagcgc
    81541 ggacaagcta tatcgaattc aatcaacgtg aaaagaattc accattcaac aatgtgaacc
    81601 taagaaaagc tatttcttat tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg
    81661 acggatcagt tgtctctaca ggattgatcc catcagatat ggtaaaaagt ccaacaacga
    81721 acgaagactt tgcaaaagaa gctggaaaat tagtttctta tgacaaagat aaagcaaaag
    81781 aatattggga aaaagcgaaa aaagaattgg gcgttgattc tttagaattt gatttgatgg
    81841 cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa
    81901 acttggatgg cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt cttgaccgct
    81961 caacttcggg tgattttgac gcagtactag gcggatgggc tgctgactat gcagacccaa
    82021 gcagctttac tgatttgttt gtcacaggta attcatacaa ccgtggtcgc tggagcaatg
    82081 cagaatatga cgaagcagtt aaagcagctt catcaacaga cgctggcgat gaacaagccc
    82141 gttgggatga tttccaaaaa gcaatcaaga tcatctctga tgatatgggt gtcgttccgg
    82201 tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat caaaggtatc gttcaccatg
    82261 cggcaggcgc ttcatgggat tacaaatgga catacgttga agaataatcg ttaataccgt
    82321 gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat
    82381 cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc
    82441 agccaaaagc tgtacatacc gtagctggca gacgagagcg aggaaaattc tggttttttc
    82501 tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa
    82561 gaaaagaccc ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca
    82621 ttagataatt gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga
    82681 atcagtagaa aagctactgc gtaggggata gatttttttt tcatttgatt gctcctttat
    82741 ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa gctaggggga
    82801 aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca tgatcatcac
    82861 gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg gcacgccata
    82921 tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac aagtcggttt
    82981 ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc aaggagattt
    83041 tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg
    83101 accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag ggacgatttt
    83161 aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta cactcgtagc
    83221 gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat atatctttgc
    83281 aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca caatcttacc
    83341 aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc gtacggaaat
    83401 ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat tgagccgttg
    83461 ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga ctttattagg
    83521 accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct ttgctattcc
    83581 tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta cgatcatggc
    83641 tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg atctattgta
    83701 cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa tggaaatgat
    83761 accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc cattacaaaa
    83821 aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta tccaagattc
    83881 atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac tattggtcat
    83941 cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg cccaaaacgt
    84001 ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat tgaatggaaa
    84061 agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt
    84121 aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct tatttatggg
    84181 aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa cgattggtgt
    84241 agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga tgcaacgttt
    84301 tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt tggtcgtttt
    84361 tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga taccaatggc
    84421 acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt tagctggatt
    84481 gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga atatctctag
    84541 tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg aagccttttt
    84601 aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga tgttgagcga
    84661 agggtacaag acattcctgt atctgcctta tctattatgg attccggcag taactttatc
    84721 ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct ttgatcctaa
    84781 aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga aatctctttt
    84841 gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt gaataaagga
    84901 gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac cagaagcatc
    84961 atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt taaaggtaac
    85021 gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga aatagcgatg
    85081 attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa gcaagtcgct
    85141 gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc tgcattagaa
    85201 ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta tccgcatcag
    85261 ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg ttatccggaa
    85321 atcctgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc acagattttg
    85381 gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat cactcatgac
    85441 cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt
    85501 gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac ttggggactg
    85561 cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat tcctggttct
    85621 ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa tgaattcgcg
    85681 atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac acacaaggca
    85741 gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga aatccaacgt
    85801 cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg aattgctgat
    85861 gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa
    85921 tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa cattcggttt
    85981 agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc gcttgaatga
    86041 acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca aagataaaaa
    86101 aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat acgcttcgtt
    86161 gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta atggactagc
    86221 taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg ttggattgaa
    86281 tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc agcgtattgg
    86341 tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac caatttctgc
    86401 actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc aaaaagaaca
    86461 tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata ttagtgaccg
    86521 aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg aaatctataa
    86581 tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac cagatccaga
    86641 ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg gacaagtacg
    86701 tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg aagtcccata
    86761 ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact aggagggaca
    86821 tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa acagcaattg
    86881 attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa
    86941 agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt aaaaaaagaa
    87001 tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct taatttggat
    87061 cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa aacgaaaaaa
    87121 agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct ggaaaatggc
    87181 tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg gccagttgaa
    87241 ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta tgtcgcacta
    87301 ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg gatcgcctat
    87361 catccgaaga cattgatcca aacatctcga cctctagaag taactttacc aaaatttcac
    87421 cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt ccgctccgct
    87481 caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa gagaaaagta
    87541 atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca attatctggg
    87601 aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga agaggcacat
    87661 gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta taagtcccaa
    87721 tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag caattccgta
    87781 agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg
    87841 aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta atgacttttg
    87901 tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca ataaaacaat
    87961 agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta
    88021 cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt
    88081 ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg
    88141 agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa
    88201 tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag
    88261 aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga
    88321 acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga
    88381 ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag
    88441 atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag
    88501 cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag
    88561 ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc
    88621 tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt
    88681 tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa
    88741 ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac
    88801 atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga
    88861 taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct
    88921 ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta
    88981 ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa
    89041 atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat
    89101 actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg
    89161 ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa
    89221 taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca
    89281 atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat
    89341 agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata
    89401 tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag gatagattga
    89461 acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct
    89521 ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc
    89581 ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac
    89641 ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt
    89701 tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa
    89761 caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg
    89821 tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg
    89881 attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt
    89941 acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac
    90001 aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg
    90061 gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag
    90121 accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa
    90181 acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa
    90241 cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg
    90301 atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg
    90361 aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga
    90421 tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc
    90481 atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc
    90541 ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg
    90601 ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg
    90661 atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt
    90721 gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta
    90781 cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata
    90841 cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag
    90901 aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc
    90961 atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc
    91021 cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac
    91081 actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac
    91141 gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa
    91201 caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa
    91261 aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact
    91321 gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc
    91381 aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg
    91441 ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc
    91501 aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa
    91561 aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg
    91621 tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact
    91681 tgaagccaat catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa
    91741 gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag
    91801 aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt
    91861 ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct atatttatat
    91921 ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt
    91981 gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat
    92041 gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt
    92101 tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata
    92161 ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa
    92221 caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac
    92281 ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat
    92341 ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat
    92401 accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac
    92461 aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga
    92521 taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg
    92581 ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata
    92641 agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga
    92701 aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa
    92761 agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga
    92821 aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct
    92881 accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct
    92941 agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc
    93001 tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta
    93061 aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc
    93121 ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg
    93181 aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct
    93241 gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg
    93301 attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa
    93361 ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg
    93421 agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg
    93481 gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt
    93541 tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat
    93601 catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc
    93661 ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat
    93721 acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt
    93781 aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag
    93841 ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg
    93901 atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa
    93961 aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt
    94021 gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca
    94081 ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa
    94141 cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag
    94201 atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc
    94261 tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga
    94321 ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg
    94381 agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga
    94441 aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc
    94501 gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt
    94561 accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa
    94621 agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc
    94681 aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga
    94741 tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg
    94801 atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt
    94861 agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc
    94921 cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta
    94981 tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac
    95041 atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag
    95101 agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag
    95161 atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg
    95221 cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa
    95281 gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag
    95341 ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag
    95401 aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta
    95461 gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa
    95521 aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga
    95581 agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac
    95641 atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat
    95701 ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg
    95761 aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga
    95821 atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc
    95881 gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt
    95941 gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag
    96001 gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg
    96061 cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta
    96121 gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt
    96181 caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag
    96241 ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg
    96301 acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg
    96361 ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca
    96421 aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa
    96481 gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga
    96541 cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt
    96601 cctcatgttt ttcgtttgtt tgggcttatt accgcagatg caagcttttg aacaccgttt
    96661 aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac
    96721 agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat
    96781 tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat
    96841 caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt
    96901 gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat
    96961 taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg
    97021 attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc
    97081 tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca
    97141 gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca
    97201 ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat
    97261 ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc
    97321 aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac
    97381 tttattggta atatgcctga aacatattat tctcaaggat atttttatac agtattcatc
    97441 ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata
    97501 gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat
    97561 cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa
    97621 aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta
    97681 aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt
    97741 gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc
    97801 gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga
    97861 accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga
    97921 tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg
    97981 acgaataccg aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt
    98041 tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt
    98101 cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg
    98161 tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac
    98221 gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt
    98281 ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc
    98341 taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc
    98401 caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc
    98461 tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata
    98521 tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc
    98581 gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt
    98641 tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt
    98701 agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga
    98761 tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat atacgttttt
    98821 cagagccatt tctaccataa tacgttccac actttctttt ttagttttat taactattta
    98881 aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt
    98941 ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac
    99001 actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt
    99061 gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat
    99121 attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac
    99181 cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc
    99241 catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca
    99301 gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt
    99361 agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga
    99421 ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt
    99481 atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata
    99541 atatgcgata taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt
    99601 tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag
    99661 agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg
    99721 taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg
    99781 agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat
    99841 tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa
    99901 tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag
    99961 ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct
   100021 ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc
   100081 gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca
   100141 acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg
   100201 tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca
   100261 tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg
   100321 ctaaataaaa aacaagcagt cttcccatcc cagaagactg cttgttttta ttgtgctgtg
   100381 aattgtgctt gatagagccg tcggtaataa ccatcttttt gaagcagctc atcatgggtg
   100441 cccatttcga caatagctcc ctgatccatc actaatatct ggtccgcatt tttgattgtg
   100501 gacaggcgat gggcaatcac aaaactggtt tttccttcca tcattgctaa gaaggcttgt
   100561 tggatttttt tctctgtcaa tgtatcgact gagctggtag cttcatctaa gatcaacata
   100621 gggggattac tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc
   100681 tttaaccctt gaccgccaat cttagtatct aatttttccg gcaaacgttc tacaaattca
   100741 tacatataag aggcttttag ggcatcattg atttctgcgt cacttgcttc cggattccca
   100801 aaacgaagat tttctcgcaa tgtactgtca aatagccaag tatcttgtaa caccattcca
   100861 aaacttttac gtaatgtatc acgttggata gagcggatgt catggtcatc gactgtaatt
   100921 gttccttttg ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga ttttccggcc
   100981 cctgtttttc ctactatagc aatggtttga cctggataag cagtaaaatt gaaatcttcg
   101041 atcaaacgct gcttggggtc ataagaaaaa gaaacatgat caaaagagac ttttcctttg
   101101 atatttttgg gttccaaggc ataggcatct ggcaaatcag gcttttcttc tggctgatcg
   101161 atcaaatcaa atgctcgttc tagaccagaa aaagcggttt gcagttgtgt cgtgattcca
   101221 gaaagttcga taaatggttt agaaaactga cttgagtaga tcgtaaagct ggaaatcaca
   101281 ccaacagtga ttgacgaacc actcttcaat gccaataggc cgccaactaa accaatcgat
   101341 aaataagcaa ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt
   101401 gcccgttgtc ctttttcata tagatcagca tttagcagtt caaattgctg ttgactagct
   101461 tcttcttgct gaaaagcttt cacgattttc tggttaccga ttcgttcggt cacaaagccc
   101521 gagatatccc caataatcct ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg
   101581 atccaactca caaggaaaat aattggtgta gaacaaagaa cgaccaatgt cagaagtgga
   101641 cttagacgca tcataaaaat aaacgaaaga atgatgattg ccactccaga aaataattga
   101701 ttgaaggccg cagaaattgc aatggaaata ttgtccatat catttgtaaa tcgactgacg
   101761 atatttccgt gggagttttg atcataataa cgaagtggca atcgattcag atgagcaaat
   101821 gcatcttttc ttaattttgc aacggataag taagctaacc gattgcttaa cacttggatg
   101881 agccattgac tgactacgtt gattaaaacg ataccaccga atagaataag taaatggaac
   101941 agtcgattga agcggacctg tccttctcca atcatttgat cgatactttg accaatttgt
   102001 aacgtcatca caactgttgc aatcccattg atgattccta gtatcacagc tagccaaatg
   102061 tcttttgcat aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt
   102121 tttactttca taactattct tcctcctgtg atgcgacgat ttcctgatat tctgctgatt
   102181 gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt accttgatcc atcactaaga
   102241 tatgatgtgc ttcttgaata gagcggacac gttgggagat aataatcact gtactgccaa
   102301 gataagtctg tagcgcctta cgcaaatcca agtctgtttg ataatccaac gcacttaaag
   102361 agtcatctaa aatcagtaat gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct
   102421 gtttttgtcc gccagagaaa tttttcccat tttccatgac ttttgtatct aaaccatttg
   102481 gaagctgacg gacaaattct tctgcctgag cgattttcag agcttcccaa cattcttttt
   102541 ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat agtcccggta aatagtacag
   102601 aagtttgggg aacaagagca atttgctgac ggagatgatc cagctgccaa gaacgaacat
   102661 ccattccgtc cattataact gtaccggaac tgacatcata gaaacgcgga atcaattgtg
   102721 tcaaagtcgt tttcccgctt ccggttggac caatgattcc aaggactgta tttcgcttca
   102781 gctgaaatgt aatattttgg agagaaagac cagaatcagg agtataacgg aagtcaacat
   102841 ggtcaaaagc gataattgct tcagaagaaa aatcgggagt agtactgacc tcagcttcaa
   102901 gaatcgaatt ttctgtgtct agcacctctt tcacccgatt tcctgaagct tcggcacgtg
   102961 tgaagatcac gacgaggttt gaaacaacga tcagtgccaa aagcatttga ttcatataat
   103021 tgatcaaagc aagaacttgt ccttgttgca agctaccaat atttactttg atcccgccaa
   103081 ctgtcagcaa acagatgatc ccgacattca tgatcaaggt tgtagcaggt gataataggg
   103141 cagagatatt ggaaacacgt aaataattgt tcgctagatc atttgtgctt ttatcgaact
   103201 tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg ctaagattct
   103261 gactaacgtg ttcattgagc tgatctagtt tttcttgcac tttttggtag agaggaacgg
   103321 tttttttgat gataaaaaat aaaatgattg agaaaatagg caataatgct aagaaaaaca
   103381 gacctacttc ccagtcgatc acaaaagcca tgaccactga cccgatactt aaaaacggtg
   103441 ctctgatcaa taaacgaatg accattgcta aagctagctg aagctgattg atgtcgtttg
   103501 tcattcgggt gatcaaggta tctgttccaa aatgattcaa ctcgttgtga gagaaagtat
   103561 tgattttttt gattagctga ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag
   103621 catagtattg gcaaatgata gcacaaatca gaccaatcac agacatagcg accatccata
   103681 agcccatttc aatgattttt cctcggtcat tcatttttag tccattatcg atcaaggaag
   103741 ccatcattaa aggcaggaca agttcgaaga cagcttcaag aaatttgaag aacgggccca
   103801 aaatgatttg cttacgatag tcttttgcat aacgtaataa ctgaatcata tgattcctcc
   103861 tattatttat ttgtccacaa ttgaaaaaac aagtactacc acattgtagc ttaggaaatt
   103921 tagaatgaaa agagaacaag ccttttcata aatgaaagtt ttattatgaa gaagctttag
   103981 aaattgaata gactattttt tttaaaaaaa agaaaatgag aaaacatgcc agtctcttca
   104041 ttcataggaa cgtctatttt acttaatttt tctataaatg aattctgcat aaaaaatata
   104101 tcaagtaaat gtttatagat gaaactctat ttacttcttt tttaatttgt tcttgcaatc
   104161 ttttttgcta cgtaattttt gtaaaaaagt gggttgagag aaatctaatt gattttcaaa
   104221 gaaggagtat gaaaatgaga aaaatattta ttcgtttatt gtgggtacta tcttagagga
   104281 ataaataata gtggagaaaa ctatggtttg gcgcagattc tatcaggttt gatggaacga
   104341 agacaacctc ccgacatcaa gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa
   104401 gaaaacaagg taggaacatc aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact
   104461 aagagtggtg tacgtaatca ataaaaagga tggtgacaac agtacctctt tactctctga
   104521 aagcatcata actcgttttt cctcattatg ccaataaatt gttgttatac cacatataag
   104581 gatttgtaat agctaaaaac agacaggaaa gttgaaatct gtgaatctta ttttgtatgg
   104641 aaggatttat agaacgcaaa aatcaagata aagatgaata aaaatcctgt ttcggctata
   104701 tattgcgtat ctacgccaat aaaacgagtt tgttctgtca tagccatata aagcaaaggc
   104761 aaaaatacaa ctacagcgag ataagatccg atgatttctc gaaaagtttt ccaggaagta
   104821 tgtttcctgt ttttatattt atccgatttc attgaatagt catagcctcc ttattctttc
   104881 atatgttgaa attccagtat aaaaactgga aggaactgta cgttttttag aataaaaatt
   104941 ataagggata agtaggctgc tgtctataat aagatatatt tttcaattct ttattaaaga
   105001 ttttattgac aaaatgaatc cgttttctat gatggaggag gggtgaataa gttgttgaag
   105061 aaggaaattt tatgttactt agagaaccag acagcctttt tcgatttaga acatatgaac
   105121 gaggtcttta cagcacaaag gttagctgat cattttgggg taaaaagaaa cacagtcagt
   105181 cattatttga accagttaaa tgatgagcaa ctactagtca aaatcaattc tcgaccagtg
   105241 atttatttcc acaaagaagc ttttgaaaaa caatttttta agttacgcac gaatttttat
   105301 ttttctatcg atgatctcaa agaggaacag ccttttttcg cgcaaccaaa agacttattc
   105361 tcttttatga tcggatacga cgccagtttg aaagaaagta tcgaacaaat caaaacagct
   105421 ctttactatc cagatggcgg attaccactt ttgattacag gcgaaagtgg cacaggtaaa
   105481 agttatttag ttaatttggt ctatcagtat tgtctgcttc atgatctgct agaagattct
   105541 gcaccattca tcacttttaa ttgtgcacaa tatgcagata atcctgaact tttgactagc
   105601 aatttgtttg gacatgttaa gggagcgtac actggtgcag aagaaaataa aaaaggggct
   105661 tttgaagcag cagatggtgg gatcttgttt ttagacgagg tacatcgctt aacaccagaa
   105721 ggtcaggaaa aactgttcac ctatcttgat caaggtgtga tttatcgtat gggagaacct
   105781 aatttgtcac gccggatcaa aacacgtctc ttttttgcta cgactgaaga gattactagt
   105841 catttcttaa caactttcat tcgaagaata cctatccgaa tcgaactacc tgcacttaat
   105901 cagcgtagtc gtaatgaacg gctagaactg atttactctt tttttctcga agaacaaaga
   105961 aaaatgggga gccctttgtc agtcagtgga ccagctttgt cacttttaac aagtcagaaa
   106021 ttatccggaa acataggaga gctgaaaaat atcgtcaaag tttcagcagc aaaagcatat
   106081 gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc atcatttgca aaaagaacta
   106141 cttgcgcagc ctattactaa aaatacagca cagcaaggaa tctatattac gcaagaccag
   106201 accttagagc aattaatgga gaagcaacag cccgaacagc agcgaatcgt taaaagtttt
   106261 gagcaaatcc taattgattt caagaaaaat aactgtctgc tatattcttg cgaaggaaaa
   106321 ttaaagcaag aagtgattca attatttgat tttttgctgt ttgagaccag tagacaagaa
   106381 aaacacgagc tgctggttta ttggacacaa tctatccgag agacctttcg acaaatggaa
   106441 actgcctatc aaatcaaatt cgatggaaac agtgtctatg ccttgagtta ctatctctat
   106501 cagcgaagca ctatcaaatg gttaccggaa gaacatgata ttatccaact aatcaaagaa
   106561 ttagaacaac aagtttctca gtcttatcca tctagttatc actatgtcca acgaattctg
   106621 gaattaagta agatcaaatt agatattgaa gacacaagta tggatcggat tttactgacg
   106681 atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac tgccaaaagc gatgattgct
   106741 gctcacggtt atgcaactgc tagcagtatg gcaaatgtag taaatcgttt attacaagag
   106801 gatctatttg aatcatttga tatgccacta gatgtcacac cacagcagat tgcttcagaa
   106861 atccttgatt attgcgaaaa cagcgatgtt tcaaatggat tgatcatatt agtagacatg
   106921 ggatctctga aagaaatcca tcagtttttt aaaaaacagt tatccgtacc gcttttgatt
   106981 ttgaataatg tgacgacacc tttagcaatc acagtagggg aatgcctgca aaaaaatgta
   107041 tcattagaag agatagcaga agagactgtt cgacagatcc agccagaatg gcgactgctc
   107101 tatccagagg aaaacaaacc caaagcactc attactactt gcttcactgg aatcggtacg
   107161 gcggtccact taagtgaact gctagaaaaa agcttaccta cggcttgtca gttgaaaatc
   107221 atcccatatg aatatcagca attaaaagat aagaaaaaca gtgacccatt attttccata
   107281 tacgaagtcg taggaatgat tggcaccaca gatcctgata tcaccaatat cgactatctt
   107341 tcattagaag acctcatctc gggggaaaag atgtctgtac tagctgaatg gttggacact
   107401 actatgaatg cttcagagaa ggaaatattc aatcagcgca tcattcgtaa tttttcattg
   107461 gaaaaagtac tggattccgt gactattcta gatacagaga aggtgatggg agaaatcgat
   107521 cattttatga gagaattaga ggtgcagttg aatcgaagca tcagcaatcc caaaaaatta
   107581 gcgttgtatg tccatgtgag ctgtctcatt gaacgattga tccgacaaat gccaatcgaa
   107641 acctatcaag gattagagaa atggaagcag tgtcaaaaag aagggttatc tgctattaaa
   107701 tctgcgttta gtgtcattga agagcattat agtgtcatga tccccaattc tgagattgcc
   107761 tatatctatg atattttgag tgaagataca gagtttctct cgatagaaga agaattttga
   107821 acataagcaa gttgttgcca ctaattttgg catgcaactt gcttttttag tatgtgtaag
   107881 tgaaaagagg agatgaaaaa gtggatagag aaattatctt agcttcccat ggaaaatttg
   107941 cttcagggat attagattca ttagagctga tctatgggaa aaatcattct attactgtat
   108001 tcgattgcta tactgatgag aattttgatt tagcggaaac agtaaggcag ctttttatga
   108061 ggtacaaaga taaagaaatc attgttctga cggatctttt tggcggaagt gtcaacaacg
   108121 aattcttaca atatatcaat cgggatcatg tctatctcgt tgccggattg aatttgccac
   108181 tattgattga gctagtatct cggttggacg ctgaagtaga gacaactgtt ttgatccagc
   108241 aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa cgagcggctc aatcagaaaa
   108301 tagaagaaga cgaattttag gaggaagaaa aatgattgta ttgacaagag tcgaccatcg
   108361 gttattacac ggacaagtag ctttttcatg gacacaaaca ataggcgcag actgtatttt
   108421 gattgctaat gatgatgtac ctacgaatga gatacgaaag acaacgatca aattagccaa
   108481 gccacaagga gtcaaactag tgatcaaatc aattgatgac tcgattgccg cgttgaaaag
   108541 cggggtgaca gataaatata agttattcat cgtagtggaa tcgatcgagg atgcatataa
   108601 gctggcagaa gcgtatccag aaatcaaaac catcaactta ggagggatca aggcaaaacc
   108661 tggtactcgc agcatcggaa aagcggtaaa tattttacca gaagaggagg aacgcttgaa
   108721 acgattaatt gacaaacaaa tagaagtaga aatccgtcaa gtaccagcag ataaaaaagt
   108781 ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat gattgtacaa gcagtcttat
   108841 taggaattgt tgcatttgtc gcacaatcag aatatgcact agggacatcg ttgctttctc
   108901 gtccgattgt gacggggctg tttacaggaa tcgtgttagg tgatgtaaaa actgggatca
   108961 ttatgggcgc aactttagag ttagcattta tcggatcgtt ctcagtaggt gcttcgattc
   109021 cacctgatgt tgtgaccggt gggattttag ggacggcttt tgctattaca gctggagccg
   109081 gtacagaaac cgcgctttta ctaggtttgc cgattgctac gttgacattg atattgaaaa
   109141 atatttactt agggcttttg attccaatta tgaaccataa agcagacaat tatgcagaag
   109201 aaggaaatta taaaggagtc gagcatatgc acttgcttgc cggtatcggt ctttccttga
   109261 tgcttggtgt tgtggtaatg gtttcttata tggtgggaag caatacgatc ggaaatctat
   109321 taaatatgat tcctgacttc gttcaaaagg ggttgtcagt agctacaggg ttgattccag
   109381 ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa acaagttgca ccatattttt
   109441 tcttaggttt tgctatagct tcttacttag agattcctgt gaccggagta gctatttttg
   109501 gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt ccgtcaacta ccacttcaaa
   109561 caactgcagg ggaggtagaa gaagatgaag aattctgaga tcaaaaacag caaaataacg
   109621 aaaaaagagc taaatcacgt attttggcgt tcttttcaaa tggagttttc ttggaattat
   109681 gaacgtcaga tgaatatggc ttatgtttat gcaatgattc cgattttgaa aaagttatat
   109741 cagtcgaaag aagaaatggc agcagctttg aaacggcatc tagagttttt taacacgacc
   109801 ccacatatcg ttacactgat tttaggaatc acggctgcaa tggaggaaga aaataaggat
   109861 gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa catctttgat gggacctctt
   109921 gcagggttag gagattcttt cttttgggga actttgcgat tgattgccac aggtgtgggt
   109981 acctctttag ccatgcaagg aaatatttta ggccctatct tgttcatttt gattttcaat
   110041 attccgcaca ttctgtttcg ctatctagca acaggttggg gatatcgctt aggaacgggt
   110101 tttttgaaaa agatccaggc aaacggtatg atggaaagtt tgacattagg cgcgtctatt
   110161 attggactga tggtcgttgg agcaatgact gccacgatga tcaccatcaa tattccttta
   110221 aaaatcggtt caggagaaaa tgctgtgact gttcaaagta tttttgatga tatcgttcca
   110281 aatatcttat gtcttggagc atttggcata gtgttttatc tattgaagaa agaagtgaaa
   110341 ccattaacga tcttgatcgg actagctttc tttggaattt ttggttcgtg gatagggatt
   110401 ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat tatatctatg aagaaaagga
   110461 agtactaagt acgatcttga aaaagaatga tttttcgaca agagaacata tgaaaaaaac
   110521 agtgaattta ctgatcttag caacgggttc atcttataac gcttgtttag cagcgaagcc
   110581 agcattggaa tcttatggtg atcttactgt cgatattcaa gaaccttttt attttttcaa
   110641 ttatggaaaa ctttctcctt caatcgatac tgtgattgct gtgtcacaaa gcgggaaaag
   110701 tgcttctact gttgaagcag taaaaatgat acaaaaacaa gggttacctg ttgttgctat
   110761 cacaaatgac gtgcaaagtc cacttgcttt agaagctgca caaattattg atttaggtgc
   110821 cggggtagaa tcagtaggct tcgttacaaa aggctattct gcgactgttt tacagttact
   110881 actcttaggg ataggaattg gaataagtaa aaataaaatc agcaaaaaaa tagaacaaga
   110941 ctacatgcaa cagttgcgca aaataatcaa ccatttacca gcgattattc aaaaaacgga
   111001 agaatttttt gatggatatc aaagtttatt tcgtttagca cagcgtttcg tcaccattgg
   111061 atatggtcca aactggggaa cagctaaaga agcagagacg aaattgactg aaacaatccg
   111121 cgttccgtca caaggatttg agttagaagc ttatatgcac ggcccatacc tggaggcaga
   111181 tgcttcgcat ttgctattct ttattgaagg ggaaagtgta aataaggaac gttcgcaaaa
   111241 gttgcaaaga tatatgagtc ggtacgttgg agagactctc accattacca caaaaaaagc
   111301 aagaaacgag aaaacacttg gattagcaat agaatgtgat gaatatctgt ctgtattagc
   111361 gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca gtagctaaag gtattgactt
   111421 gaacaaaaag atttttgaag actttgatac agtcttgaaa agtaaattat aagaggtgga
   111481 aatcatgctg aaattcaatg aagaagaaca aatcaaagca ataaaaggcg cactagcttt
   111541 acgtccgcaa gtagaagaaa tcatcgataa actttatgcg gaaaaatttg acgctgtcta
   111601 ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa gctgtcactt atatgaatgg
   111661 gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa tattacacta ctggaaacaa
   111721 aagattaaca aaagattcta tcgttatttt gtcctctgtt accggtacaa ctcaagaagt
   111781 agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca ctgattggat ttatcgataa
   111841 agcgaacagt aagttgtctc agctatgcga ttttgtggtc acttatccag ctcctggaac
   111901 agaacaaatc aaatttttta tggcagcaga tcgattgatg tatttgaatg gagaattcga
   111961 agcttattca gaatactatg aacagctcga acgttattta ccaacagggt tagtggaagc
   112021 agagaaaaaa gcagatgcat ttggcttatc atttgcagaa aaacatcgac gtgatagtat
   112081 gcattatttt attggagcag ggaatcaatg gggagctgtt tattcatatg ccatgtgtta
   112141 ctgggaagaa caaagctggc ttccatcgaa atctatccat gctgccgagt ttcttcacgg
   112201 gacgttagag atcgtagaag aaacaacccc agtcacatta tttttaggcg aagatgaaca
   112261 gagagtgtta tctgaacgtg tggctaaact tttgccaaaa atttgcagca attatacatt
   112321 gatagacaca aaagactatc cagtagaagg tatcagtgaa aaatatcgcg gacgtgtttt
   112381 gtctttctta ttgatgcatg tagtgacaca acgcatcgat gctcatgtgg agcagttgaa
   112441 ctgccatcca ttagagatac gccgctacta tcgtcagttt gattattagg atagactgga
   112501 taaaaagaat ataaaaaata aagagagcag cgataactgc tccctttatt tttttaaaat
   112561 attggcatca attacgttat aaaacacatt ttttgcatta tctacagttc aacttataat
   112621 ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta tgcaatagta ctatttagtt
   112681 gttgcatgga tccgtagtaa aagtccaagt acaacgagtt gcgccagttt acatagcgtt
   112741 ttgtatccag aatgttgact aaaacaaaag ttcctatgtc aaccgtcaac agagacaatc
   112801 tgtccgttgc aggacctgac agatttagaa atgttcaatc tgtctcttat gtattttaaa
   112861 tcaatactca ctaggagaag tttcctaacc tcctaatagt aatgctggta gtaacattgt
   112921 caaaactgct tcctcttagg aacaagcaaa gcataacgag tgtaaaaata aaatgtagac
   112981 tattgtaaca tatcattgat tatttcaagt aaaaacgata aaagaagtaa acaaacactt
   113041 aaaaacaatg gaaattagtc ccaactttaa gaaatgtata ctataatgga aattatcaat
   113101 tatttatttg gaggaatatc catgaaacaa aaaatcgcag ttcttggtcc tggatcttgg
   113161 ggcactgctt tagcacaaac attggcagaa aacgggcatg atgtccgtat ttggggaaat
   113221 gtacctgaac agattgatga aatcaataag tatcatacaa atcagcattt tttacctgat
   113281 ttgacgatcc cagaatcgat tatcggatat aaagaattgg cagaagcagt ggataacgca
   113341 gatgctattt tatttgttgt tccaacaaaa gcgattcgtt cagtcgcaca ggaacttatt
   113401 ggtaaaatga atactaagcc aatcattata catgcaagta aaggactaga acaagatacg
   113461 cataaacgaa tctcagaagt tttggctgaa gaaattccag aagaaaaaag acaggcaatt
   113521 gttgtattat ctggtcctag ccatgctgag gaagtggcag tacatgatat cacaacaatt
   113581 acagctgcaa gtattgatca aaaagcagca gcctatgtac aaaaactgtt catgaatgat
   113641 tatttcagga tttatacaaa taatgatgtg attggcgtag aaactggtgc agcactgaaa
   113701 aatatcattg caattggtgc cggcgctatc catggattag gattcggaga tgatgcaaaa
   113761 gcagcaatca tgactcgagg attggcagaa atcagccgtc ttggtgtcgc gatgggtgcc
   113821 aatcctctga catttatcgg tttgagtggt gtgggggact tgatcgttac ttgtacaagt
   113881 gttcattctc gcaattggcg tgccggtaac ttgttaggac agggacataa gctggaagaa
   113941 gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa cgacaaaagc agcagtagag
   114001 ctagctcaac agctaggtgt tgagatgcca attactcaaa caatctataa tgttttgtat
   114061 aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt tacgtgacgg aaaaatggag
   114121 aatgaatttt ctcttcgtta aatagatgat caatagttaa tcgt
//
LOCUS       DAPBAB010000003       110572 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPBAB010000003 DAPBAB010000000
VERSION     DAPBAB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 110572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 110572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..110572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.003"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(620..976)
                     /locus_tag="RD444_000264"
     CDS             complement(620..976)
                     /locus_tag="RD444_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDT7547670.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(990..1400)
                     /gene="spx"
                     /locus_tag="RD444_000265"
     CDS             complement(990..1400)
                     /gene="spx"
                     /locus_tag="RD444_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDT7547671.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1752..3938)
                     /locus_tag="RD444_000266"
     CDS             complement(1752..3938)
                     /locus_tag="RD444_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDT7547672.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4170..4853
                     /locus_tag="RD444_000267"
     CDS             4170..4853
                     /locus_tag="RD444_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDT7547673.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4934..5653)
                     /locus_tag="RD444_000268"
     CDS             complement(4934..5653)
                     /locus_tag="RD444_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7547674.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5965..6171)
                     /locus_tag="RD444_000269"
     CDS             complement(5965..6171)
                     /locus_tag="RD444_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547675.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6416..7018
                     /locus_tag="RD444_000270"
     CDS             6416..7018
                     /locus_tag="RD444_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDT7547676.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7159..8439
                     /locus_tag="RD444_000271"
     CDS             7159..8439
                     /locus_tag="RD444_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7547677.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKTLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGDSAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8452..9180
                     /locus_tag="RD444_000272"
     CDS             8452..9180
                     /locus_tag="RD444_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7547678.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9249..10772)
                     /locus_tag="RD444_000273"
     CDS             complement(9249..10772)
                     /locus_tag="RD444_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDT7547679.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVASI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFITQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYIEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            11024..12205
                     /locus_tag="RD444_000274"
     CDS             11024..12205
                     /locus_tag="RD444_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7547680.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYQNSSFSEEDYF
                     K"
     gene            complement(12265..13893)
                     /gene="treC"
                     /locus_tag="RD444_000275"
     CDS             complement(12265..13893)
                     /gene="treC"
                     /locus_tag="RD444_000275"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDT7547681.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14414..17125)
                     /locus_tag="RD444_000276"
     CDS             complement(14414..17125)
                     /locus_tag="RD444_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547682.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNAKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17135..17836)
                     /locus_tag="RD444_000277"
     CDS             complement(17135..17836)
                     /locus_tag="RD444_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547683.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            18021..18620
                     /locus_tag="RD444_000278"
     CDS             18021..18620
                     /locus_tag="RD444_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7547684.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILSNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTNN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHVVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18701..19714
                     /locus_tag="RD444_000279"
     CDS             18701..19714
                     /locus_tag="RD444_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7547685.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19823..20740
                     /gene="rbsK"
                     /locus_tag="RD444_000280"
     CDS             19823..20740
                     /gene="rbsK"
                     /locus_tag="RD444_000280"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDT7547686.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20768..21163
                     /gene="rbsD"
                     /locus_tag="RD444_000281"
     CDS             20768..21163
                     /gene="rbsD"
                     /locus_tag="RD444_000281"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDT7547687.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21163..22536
                     /locus_tag="RD444_000282"
     CDS             21163..22536
                     /locus_tag="RD444_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7547688.1"
                     /translation="METTMNPEQMVLYEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRDNMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22893..25043
                     /locus_tag="RD444_000283"
     CDS             22893..25043
                     /locus_tag="RD444_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7547689.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNN
                     NNGSNNSNNNNNNSNNGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPN
                     TANQSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFEHLGKPYVWGAKGPN
                     TFDCSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVG
                     IYIGNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPATSGSLTGSSILDGLDGS
                     FHFSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLF
                     GIKGSYNGSSVTFNTHEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDF
                     YKPTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEA
                     NAEILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQ
                     IPETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(25095..26135)
                     /locus_tag="RD444_000284"
     CDS             complement(25095..26135)
                     /locus_tag="RD444_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7547690.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSLDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26411..28213)
                     /locus_tag="RD444_000285"
     CDS             complement(26411..28213)
                     /locus_tag="RD444_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547691.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQTTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28227..28979)
                     /locus_tag="RD444_000286"
     CDS             complement(28227..28979)
                     /locus_tag="RD444_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547692.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28988..29350)
                     /locus_tag="RD444_000287"
     CDS             complement(28988..29350)
                     /locus_tag="RD444_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7547693.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29526..30398)
                     /locus_tag="RD444_000288"
     CDS             complement(29526..30398)
                     /locus_tag="RD444_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDT7547694.1"
                     /translation="MREKRAFYINGYAGIVGLIVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30449..30937)
                     /locus_tag="RD444_000289"
     CDS             complement(30449..30937)
                     /locus_tag="RD444_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDT7547695.1"
                     /translation="MKAEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(31075..31866)
                     /locus_tag="RD444_000290"
     CDS             complement(31075..31866)
                     /locus_tag="RD444_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDT7547696.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADYELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31921..32529)
                     /locus_tag="RD444_000291"
     CDS             complement(31921..32529)
                     /locus_tag="RD444_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDT7547697.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32522..32839)
                     /locus_tag="RD444_000292"
     CDS             complement(32522..32839)
                     /locus_tag="RD444_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7547698.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32996..33724)
                     /locus_tag="RD444_000293"
     CDS             complement(32996..33724)
                     /locus_tag="RD444_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDT7547699.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33884..34765
                     /locus_tag="RD444_000294"
     CDS             33884..34765
                     /locus_tag="RD444_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7547700.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITDFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(35005..36045)
                     /locus_tag="RD444_000295"
     CDS             complement(35005..36045)
                     /locus_tag="RD444_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7547701.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRVIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQVNGKIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAIIRLSK"
     gene            complement(36048..36716)
                     /locus_tag="RD444_000296"
     CDS             complement(36048..36716)
                     /locus_tag="RD444_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7547702.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     HIELIITDVMMPEMDGYDLVGSLRNARFDMPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSILTIGQTKLELDTYTVVQNQHSFILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36858..39194
                     /locus_tag="RD444_000297"
     CDS             36858..39194
                     /locus_tag="RD444_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7547703.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSD
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39309..39710)
                     /locus_tag="RD444_000298"
     CDS             complement(39309..39710)
                     /locus_tag="RD444_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547704.1"
                     /translation="MEWMRFFDNRINYDILINKRKRILSVGGRKVKKMEESFPKAAKV
                     ENIANILKVTFENGEVKYVKSHWTEEITDDLQFGKKGRGKRKNLLALSRNMWIGTEVT
                     IEADGTVFINGKDRYTPEELWYKGKKSIPEL"
     gene            39801..41276
                     /locus_tag="RD444_000299"
     CDS             39801..41276
                     /locus_tag="RD444_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDT7547705.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDVIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVKDWEKYLK"
     gene            41323..41907
                     /locus_tag="RD444_000300"
     CDS             41323..41907
                     /locus_tag="RD444_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDT7547706.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLNEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            41909..42328
                     /locus_tag="RD444_000301"
     CDS             41909..42328
                     /locus_tag="RD444_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDT7547707.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42609..42893)
                     /locus_tag="RD444_000302"
     CDS             complement(42609..42893)
                     /locus_tag="RD444_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDT7547708.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43245..43955)
                     /locus_tag="RD444_000303"
     CDS             complement(43245..43955)
                     /locus_tag="RD444_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDT7547709.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43952..45424)
                     /locus_tag="RD444_000304"
     CDS             complement(43952..45424)
                     /locus_tag="RD444_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7547710.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLTKAGDNA"
     gene            45562..46035
                     /locus_tag="RD444_000305"
     CDS             45562..46035
                     /locus_tag="RD444_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7547711.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46188..46739)
                     /locus_tag="RD444_000306"
     CDS             complement(46188..46739)
                     /locus_tag="RD444_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7547712.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46844..47374
                     /locus_tag="RD444_000307"
     CDS             46844..47374
                     /locus_tag="RD444_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDT7547713.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47860..48303)
                     /locus_tag="RD444_000308"
     CDS             complement(47860..48303)
                     /locus_tag="RD444_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7547714.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48319..49479)
                     /locus_tag="RD444_000309"
     CDS             complement(48319..49479)
                     /locus_tag="RD444_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7547715.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTIAEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            50355..52055
                     /locus_tag="RD444_000310"
     CDS             50355..52055
                     /locus_tag="RD444_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547716.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNIRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFNKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKHLRA
                     "
     gene            complement(52157..52990)
                     /locus_tag="RD444_000311"
     CDS             complement(52157..52990)
                     /locus_tag="RD444_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDT7547717.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLAKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEEGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(53540..55705)
                     /locus_tag="RD444_000312"
     CDS             complement(53540..55705)
                     /locus_tag="RD444_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7547718.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTSGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNRQADS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLEVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55965..58259
                     /locus_tag="RD444_000313"
     CDS             55965..58259
                     /locus_tag="RD444_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDT7547719.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEATITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            58252..58917
                     /gene="pgmB"
                     /locus_tag="RD444_000314"
     CDS             58252..58917
                     /gene="pgmB"
                     /locus_tag="RD444_000314"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDT7547720.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDRTFNEQLK
                     GVSREDSLSLLLAHGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58910..59917
                     /locus_tag="RD444_000315"
     CDS             58910..59917
                     /locus_tag="RD444_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7547721.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEELPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRKMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59939..60958
                     /locus_tag="RD444_000316"
     CDS             59939..60958
                     /locus_tag="RD444_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7547722.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(61239..62606)
                     /gene="nhaC"
                     /locus_tag="RD444_000317"
     CDS             complement(61239..62606)
                     /gene="nhaC"
                     /locus_tag="RD444_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDT7547723.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(62715..64136)
                     /locus_tag="RD444_000318"
     CDS             complement(62715..64136)
                     /locus_tag="RD444_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7547724.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(64379..66256)
                     /gene="tdc"
                     /locus_tag="RD444_000319"
     CDS             complement(64379..66256)
                     /gene="tdc"
                     /locus_tag="RD444_000319"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDT7547725.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FTDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(66547..67803)
                     /locus_tag="RD444_000320"
     CDS             complement(66547..67803)
                     /locus_tag="RD444_000320"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDT7547726.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67915..68120)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            68383..69684
                     /locus_tag="RD444_000321"
     CDS             68383..69684
                     /locus_tag="RD444_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDT7547727.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69753..70352)
                     /locus_tag="RD444_000322"
     CDS             complement(69753..70352)
                     /locus_tag="RD444_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7547728.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            70606..71487
                     /locus_tag="RD444_000323"
     CDS             70606..71487
                     /locus_tag="RD444_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547729.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            71500..73110
                     /locus_tag="RD444_000324"
     CDS             71500..73110
                     /locus_tag="RD444_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDT7547730.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(73187..73846)
                     /locus_tag="RD444_000325"
     CDS             complement(73187..73846)
                     /locus_tag="RD444_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7547731.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(74129..74794)
                     /locus_tag="RD444_000326"
     CDS             complement(74129..74794)
                     /locus_tag="RD444_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDT7547732.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(74941..75546)
                     /gene="dhaL"
                     /locus_tag="RD444_000327"
     CDS             complement(74941..75546)
                     /gene="dhaL"
                     /locus_tag="RD444_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDT7547733.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(75574..76584)
                     /gene="dhaK"
                     /locus_tag="RD444_000328"
     CDS             complement(75574..76584)
                     /gene="dhaK"
                     /locus_tag="RD444_000328"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDT7547734.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(76559..77005)
                     /gene="dhaM"
                     /locus_tag="RD444_000329"
     CDS             complement(76559..77005)
                     /gene="dhaM"
                     /locus_tag="RD444_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDT7547735.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            77108..77428
                     /locus_tag="RD444_000330"
     CDS             77108..77428
                     /locus_tag="RD444_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDT7547736.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(77540..78409)
                     /locus_tag="RD444_000331"
     CDS             complement(77540..78409)
                     /locus_tag="RD444_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7547737.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(78505..79807)
                     /locus_tag="RD444_000332"
                     /pseudo
     CDS             complement(78505..79807)
                     /locus_tag="RD444_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(79804..80313)
                     /locus_tag="RD444_000333"
     CDS             complement(79804..80313)
                     /locus_tag="RD444_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547738.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(80488..82623)
                     /locus_tag="RD444_000334"
     CDS             complement(80488..82623)
                     /locus_tag="RD444_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7547739.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(82706..84361)
                     /locus_tag="RD444_000335"
     CDS             complement(82706..84361)
                     /locus_tag="RD444_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7547740.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(84363..84677)
                     /locus_tag="RD444_000336"
     CDS             complement(84363..84677)
                     /locus_tag="RD444_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7547741.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            84847..85107
                     /locus_tag="RD444_000337"
     CDS             84847..85107
                     /locus_tag="RD444_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDT7547742.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            85121..85435
                     /locus_tag="RD444_000338"
     CDS             85121..85435
                     /locus_tag="RD444_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDT7547743.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(86165..86719)
                     /locus_tag="RD444_000339"
     CDS             complement(86165..86719)
                     /locus_tag="RD444_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDT7547744.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(86845..88773)
                     /locus_tag="RD444_000340"
     CDS             complement(86845..88773)
                     /locus_tag="RD444_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDT7547745.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLL
                     KEMRKRQEERRVNDENSK"
     gene            complement(89079..89795)
                     /locus_tag="RD444_000341"
     CDS             complement(89079..89795)
                     /locus_tag="RD444_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDT7547746.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(89796..91619)
                     /gene="glpO"
                     /locus_tag="RD444_000342"
     CDS             complement(89796..91619)
                     /gene="glpO"
                     /locus_tag="RD444_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDT7547747.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(91633..93129)
                     /gene="glpK"
                     /locus_tag="RD444_000343"
     CDS             complement(91633..93129)
                     /gene="glpK"
                     /locus_tag="RD444_000343"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDT7547748.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(93265..94725)
                     /locus_tag="RD444_000344"
     CDS             complement(93265..94725)
                     /locus_tag="RD444_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7547749.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(94715..96010)
                     /locus_tag="RD444_000345"
     CDS             complement(94715..96010)
                     /locus_tag="RD444_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7547750.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(96342..97019)
                     /locus_tag="RD444_000346"
     CDS             complement(96342..97019)
                     /locus_tag="RD444_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDT7547751.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(97049..98140)
                     /locus_tag="RD444_000347"
     CDS             complement(97049..98140)
                     /locus_tag="RD444_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDT7547752.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(98241..98675)
                     /locus_tag="RD444_000348"
     CDS             complement(98241..98675)
                     /locus_tag="RD444_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7547753.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(99259..100716)
                     /locus_tag="RD444_000349"
     CDS             complement(99259..100716)
                     /locus_tag="RD444_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7547754.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(100713..102608)
                     /locus_tag="RD444_000350"
     CDS             complement(100713..102608)
                     /locus_tag="RD444_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7547755.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(102666..104441)
                     /locus_tag="RD444_000351"
     CDS             complement(102666..104441)
                     /locus_tag="RD444_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDT7547756.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(104491..105987)
                     /locus_tag="RD444_000352"
     CDS             complement(104491..105987)
                     /locus_tag="RD444_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7547757.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(106181..107803)
                     /locus_tag="RD444_000353"
     CDS             complement(106181..107803)
                     /locus_tag="RD444_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDT7547758.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(108253..109566)
                     /locus_tag="RD444_000354"
     CDS             complement(108253..109566)
                     /locus_tag="RD444_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDT7547759.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(109811..110035)
                     /locus_tag="RD444_000355"
     CDS             complement(109811..110035)
                     /locus_tag="RD444_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547760.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(110055..110477)
                     /locus_tag="RD444_000356"
     CDS             complement(110055..110477)
                     /locus_tag="RD444_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDT7547761.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
BASE COUNT        32475 a        22776 c        19357 g        35964 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcgtttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa gcgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt tcaacgactc
      301 agttatctta tcatgtcatt tccaacatgt caacaactat tttatatgtt ttttaaaata
      361 ctattttatt gctttgctac ttaaaacaac agaaatgaat atatcatgta aatttacaca
      421 tgtcaatata tttttaactc tatatcccca cctagatttg aattttgacc acataaattg
      481 agcatgtctc tataaattga gcatgtctct atagtagaaa aataaagtca aaaaaagaac
      541 acataacacg cttgatgcta agtgttcgac tgttacaact gtatgaaatg ctcagtgact
      601 tgattaaaac agttaaagtc tatttattta agataagctg ttccatcaac tgctcattgt
      661 atccaataaa tagaacttca tcgataataa tcaaaggacg tctgaggaga ttggtatgtt
      721 gtgtgcataa aaaaatcagt tgtttttttc ctacacagga aaggtcaatc gcaatgttgt
      781 gaaaagcctc agaccaagtc gcaaatagct gttctgcttg cacttcttca aaactgcata
      841 attcttctgc ttcttctgta cttagatcgc gttcttctaa gtcaacaaat tgataaggca
      901 attgatggtg ttctaaccat tgtttcgcct tcagacttgt tacacagtgc gctgatccat
      961 agactttcac ttccacgaag gctccctccc tatgaattta agatgttatt gttttgtaaa
     1021 tagatcagat ttctggcaac acatcgaatt tcacgaggga caaatgaccg gatctcatct
     1081 tcattaaatc cgattagtat tcttttttca tcaatcaaaa taggtcgtct caataatgaa
     1141 ggatattctt tgaataagac gatcatttgc tttaaagtta attcgttaaa atctatgccc
     1201 agcttttcgt aaattttcga ccgagtggaa ataatatctt gaactcctcc ttcagtcgct
     1261 gccaagatct ttttgatctc actttctgtt aacggttcgg caaaaatatt cttctcttca
     1321 aattcaagtt gattcgcttg caaccatgct cttgcttttc tacaggaagt acagctaggc
     1381 gaagtatata cttttatcat tttctcactc ctttttaatt ataatccaat tctacagtaa
     1441 tttcttttaa aataccaatt attagctaat aaaatataaa aaaaaaaaaa atcaaaataa
     1501 ctgattattt acgtttgaat aatgagaatt attctatata ttctctattt tgataatagt
     1561 attctcattt ttaaaataaa taagggaggt gtgacaaaat tcttgtcaca cctccttttt
     1621 ccgaataaac tgtgttccag aaatagcttc ttcgaaaata agctgaagtt tcacaaaaat
     1681 ttgagaagca attttcgtac attccttctt ctttcttgaa actgaccact tctgtcacac
     1741 cctctcttct atcactttcc ttttttcaca cgactcccgg tttctttgat aaatctcgat
     1801 actttcaatt ttcttgaatt tttcatttga ggtacacata gatacagacg atttcttgct
     1861 ctagtcatcg caacataaaa tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct
     1921 tttatttcat taaagtcgat tactttcttt tctgtcgctc cgttataagg caaacttcct
     1981 tcgttacaac caatcaaaaa gacatttcgg taactaagac ctttactttt gtgaatgctc
     2041 tggatacata gtgcatcaag ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct
     2101 ttagctgtcg cttgggtttc ttgcgataag gcttggagtc ttctgaaagt agcatgctgc
     2161 tttacttctt ctcgtatcca ttcttgatca ctctggtcga tttccttagc tatacgtggc
     2221 agatgaagat agctttgaac aagcgaatag atttcttgat gctggtctgt tttacgcaac
     2281 gcttcctcaa actctcggat ttctgcagat ttcgatttta aattggggct tatcgacaaa
     2341 atataatctg caacggctat attttgatat ttaggagaaa agtattgatc gcattcactt
     2401 tttttcaggt agcagtctgc catcagctgc cggagcaatt gctggtaggc ctgatttttc
     2461 ttttgcagat tcagtgctgt ttgccaatga acaatttttt gatagaaatg ttggaaagac
     2521 ttgataccaa agataccttc atatggacta tctgcttcca gtacaaaagg aatattcgtt
     2581 ttcagcagag attggacaag ctgctgcgtt tgtgaagctg atcggtataa tatgattgat
     2641 tccttatatt tcccattttt ttgaataaat gtctgaattt gtttgattag ccactgactt
     2701 tctgctttag tatcagaaaa caaataggct tccataacac ctgcttcttt ttgtgctgga
     2761 atcatcgcct ttggtatccg atcattttta ttatagctga tcaatctatt tgctccttgg
     2821 acaatagctg cccgagaacg ataattagtt aataagaaaa gagttttggt aggatattct
     2881 ttttcaaagt tcttgatgta tgctggctct gatcctctga atccatagat actctgatca
     2941 tcatctccaa caacaatcaa cttgatcctg ttttcctttg tcatccgttt cacgatttcc
     3001 cattgaagtg gattagtatc ttgaaattca tcaatgaata gataatcata tttttggccg
     3061 atataatgct ggatctcctc attctccaaa gcgcttttca gattttggag tacatcattg
     3121 aactctagca attgctggct tcttttttgt tcttggagtt ccgtgagcaa tccatacagt
     3181 cgttctgata tcggctgtcg tgcattctta tcaatggaat catatttttc tttaactaac
     3241 tgaaccattt cttctactga aaggttcata ttctgccaat agtctaaccg agaagcaatc
     3301 gttttttctc catattgatc aaatatataa agattatatt tacgtatcag ctgttgcaaa
     3361 acagcattca ctttttgtgc agtatccttt gaaaagtctc cttgtattcc ttgcattgct
     3421 acttcaggaa attttcgaca aatttcattg tatatcctaa aaaagatact atggaatgtt
     3481 ccgatatgga cagcggtttt tctctggctg aaattatcta ctcgtttttt tagttcattt
     3541 gcagcgttcc ttgtaaaagt gatcattagt atgcgttctt cgtctacttg tttttgcgtc
     3601 atcagataaa cagcttttgc acagattgtt tgcgttttgc cgctcccagc tcctgctatg
     3661 attcgaagat gtttcttcat atcatatgtg atagctgctt tttgttcttg attgaagcga
     3721 ttataggaac gtttctccat cattttaaag aaagagctgt ttccttcact ttcttttact
     3781 tgtacttctt cctcaataag tccgggtgct tcgccataag ccgcttcata ggtagcaatg
     3841 atttcttctt gtagccgaaa gacttgttga agctttttat acttgttctc ccagcgtttc
     3901 aattcttttt gtaatcggag aatttctttt tcattcaact gattgatttc cttcccatct
     3961 ttcttctttg aatttgttta tttcaagttg gttatttaaa gttggttatt taaagttttg
     4021 ttgtctattt tttctttata ctaacagacc tttctaaaaa tgaaaatggg aagcgatatg
     4081 attttagttt tctgaaaatt tttccttttt ttcaatcgtt tatgctaaga taaggaagac
     4141 aaaaatattc ataaaaggtg atgaaaaaaa tgcagacttc ctttcacgag gggcgtacaa
     4201 taggactttt gctgacattt atcggaggag ccatggacag ttatacttat atccaatatg
     4261 gtgcttttgc ctctgctcaa actggcaata tcattctatc gatcatccaa gcatttgacg
     4321 gagaatggct aagtgttggt aaaaaagtat tgtctaccct tttctttttc ttagggattc
     4381 ttctaacaaa gtatctgatt gattatttta gaaaaaaaga aaaacatttc tggcgcttgt
     4441 ttgttctata ttatgaagca gtgatttttt tctttgttag tttgacaccc gtccatgagc
     4501 atcccgcatt agtaacgatt ttgattgctt ttactgcatc gatccagtgg gtagcttttg
     4561 ataaaatcaa cgggcgtgcc tacactaatt tatttacaac tggaaattta aaaggggtag
     4621 caacgaactt atatgattat tgtatttcta aagaaaaagc agattttgat cgatttttcc
     4681 actatctaag tgtcgttctt gcctttatcg caggagctat tgtccttgtt ttctgtcatc
     4741 atctgtttgg cgcccattcg attttgatcg tttccatttt atttttgatc atggccctca
     4801 ctcagacatt tcatttatgg caattttatc atacgaatcg gttcactatt tgatctcaat
     4861 gaatcaaaaa aactaagcaa gatgctgctc acagagaaat atctgcgagt gagcaatttg
     4921 cttagttttt atattattta gttgtaaaga ttccctttgc tttcgggtta tttgtagtgt
     4981 agcggatcga ctgggcatct gcctcttctg tcattttctt tgcccgcatc ttgatttctt
     5041 gtacgatttg ttctccacat gccggatcgg ctacgaaatc atatttgtcc ccatcgatca
     5101 ctattttggg acatatatca aactgttcat accactcttc gtatcgtcga ttgagttctt
     5161 tatagtagga atagagttca ggatcttgtt ctagctgttc atattcccgg cctctttttt
     5221 tgattctcgc taacatagtt tcaaaggata cacgaatata aaccagtaga tctggtttct
     5281 tttgtggcga aatttcttct aactccttca gcatcgtttc taaaagacct tcatactgct
     5341 ttacttctac ttcagaaaca cgccctaaat ctgcattcaa atgaaacagc atactatctt
     5401 cgtagatgga gcggtctagt acgttgtcat catggctgaa agcatctttg attgccaaaa
     5461 agcgtttgtt caaaaagaaa atctgtaata aaaaagtata cttttctgga ttagaataaa
     5521 atagcggcaa tacttcatta tcttccacgt tctcataaaa aggttttgtt tctaaatctt
     5581 gggcaagcat ttcagtcagt gtgcttttcc ctgcaccgat cgttcctgca acaatgatca
     5641 caattatcgg catccttcct gtaataaaat ctgttcttca ttttactact tcatttatag
     5701 aaataaaagc cttgacttta tctgctcctt aaatgctact tctctaaaag tagccaaaag
     5761 taactcctat tcatgaaaat aaaggctgtg acaaaagtct tgtcgcagcc cttttttcaa
     5821 ataaacagta ttctcctgcc aacacctcgg tcacaaggca atattcataa aacatgaaaa
     5881 gctgtttttt aacattattc cttgtaactc agagctaaac gggagactca caacctcttt
     5941 atgctctcaa cattttatct gtttttattc ttctattgta cttgatttgc gtacaactaa
     6001 ctgccagtgc agattatgag cggtatcatt gtcttctact gcgcatttca tgacacgtat
     6061 catcacacta ttttcataga ttttttctat ttttcctttt attggtgatt caaataaatc
     6121 agatttaaat gcatatgttt cgccgacttc aaacaattgt tcattttcca taagaatccc
     6181 tccatcacct tttataaggt cagtaatttt ttaacataaa tcactcgaaa aagcacaaac
     6241 ttgtcataaa aagtactttt cgtatcaaaa ctgtcacttt ttttggtaag gttcttggaa
     6301 aacgaagatt tttttaggaa atcatgacgg ttttgatgtc aaaaccactt tttatgaaac
     6361 aaaagacgtt ttttattttt tttagcataa aatttgtggt ggaggtacgt atgagatgaa
     6421 agataaagtt gtgaacaaat tattagaagc cttattcagc ggagacaaat cggaagaaga
     6481 tatggtgtat gtccaagtaa aatttgcctt agaagtttta ttgaataatc ttggaaaact
     6541 agcggtcgtt ctagtctttt ctctggtgac aggctcatgg gcggaaacag gaatcacctt
     6601 tctttcttat atctgcatcc gcagatatgc ttatggattg cattccgact ctgaattcgt
     6661 ttgtctgctg tggacactac tttatttatg gggcgttccc ttggtgatga aacatctgca
     6721 attgactatc tccttcccaa tgatggtctt tttgttaatc agctgctttt tgttactgct
     6781 tcgctatgga tcaagaggta cagcaatcaa tcccattgaa cccgagaaac gtcccccatt
     6841 attaaagaag gcgatcagca tgtttctaat tttttctctc attactttat ttttctcagc
     6901 ttcttacttt tctacctatt tgctacttgg gattgtttta gaaatagcta cgttgcttcc
     6961 tattacaaac tacttgatga attttggagg aatctttcat gaaaaaaaca atcattaaac
     7021 aattaactaa cttatctgac ttttttgtat ctggctgctg ctttggtatg agttctgaat
     7081 tcaaaatccc tgaaaaatta aaaaaataat acgctaattg tgctataatt tggtaaaaat
     7141 caatgggagc tgtactttat gtttttatgg tccatagcgt tgcaatattt gatttacaca
     7201 atttgtatct tattgattga ccatcacatc cgatacaaat atctgtttgc gggagtcatt
     7261 ctgatttctt gtattttgat tggatgggtc tattcgattt tagggatttt tactgctcta
     7321 attgctatag gaatgctgat gcttactttt ttcttctttt cgacttcgcg tcaacaattg
     7381 gtttacgccc ttccttttgg gttattgacg tcgcttttag gagatcactt aacaagcatc
     7441 ggcgattttt ttgttttgaa atcttcggtt attgaaccag gagattcatt gcagtatttt
     7501 cacattgcct tatccgcttt tttatctgct ttattagctt tttgttttaa aaaaacgtta
     7561 gagacttggt ttcacactgt ggaccgccgt atgatcggta cgatcgggac attgatatta
     7621 tttacatact atatcattat tttttatacc cgtttttctg gtgaaacacc tgatgtctta
     7681 gctttgaata cgagtttttt cattctatat ctgtgtgttg gcctgatcat cttggtttat
     7741 tattggaaaa tctctaataa gaaattagct gatcagttat tagagcaaca agtcaggatg
     7801 caacaggatt atatcaaaga tctagaaaaa aactatcaag acttgagaga attcaaacat
     7861 gattaccaaa atctgctttt ttctcttcac agctatattt cagaaggcga cttagaagga
     7921 ttacggtctt attatgatca aacgatatta cctacacgcc agatgatgaa cctttttccg
     7981 gccaatctaa gtatgttgga taatatgaaa gttcctgaga tccgtagttt actgtcatta
     8041 aaactcatga tggcacagga aaaagggttg gtcgtccagc ttcattttcc tgagccaatc
     8101 gaactggata cgaaacatac ggtaaacctt gtacgtatgc ttggtattat cttggacaat
     8161 gcgattgaag gagatagtgc tgcaaagaag aaaaaaatcg aattgaccat actcaagaag
     8221 aatcattccg tgatgattcg tgtgatcaat acaacaacaa atacattgcc gttaaaccag
     8281 ttgaaacaaa aagggttctc aacaaaaaca aatccaaaaa acgaaggcct tggcttgttt
     8341 atcttggatg aattgacaaa gaccaatcct tatcttttcc ttgaaacatc aattgaaaac
     8401 cagcgttttt ctcaaacgat ttatatacaa ataaaataaa ggagtaatga tatgattccc
     8461 atttatattt gtgaagacga tccaaaacaa ttagaagttt tatcaacggt gatcaaaaat
     8521 agaatcatga ttgaaaactt agacagttat gtccatattt ctacctctga tccggaagaa
     8581 cttctcaatg cagccaagat cagaacttct tcttttggta tttacttttt agatatcgaa
     8641 ctagaagaca gtgaaatgga tggtgtagcg atcggtaaat tgatccgtga attagatcct
     8701 ttaggaaaaa taatctatgt gacttcccat acggaaatgt cgatgaaaat cttaaaaagc
     8761 aacattgaac cgactgatta tatcgtcaaa gaggatcttt atgatttaaa agaaaatgtc
     8821 gagcatatcc tgagcaaaat tttcgaagat tttcaaatgt cccagatcca aaaagatatc
     8881 ttcaaaatag agtttaatga tgagatcaaa tttttaccga tcaaagacat ccaatatttt
     8941 tctacttcgc taggagcacc acataaactg gaagtccatt tgactcatgc ccaattacaa
     9001 ttctatgaga agatcaaaga aatcgagaaa atgcatcgga attttatccg ttgtcataaa
     9061 tcctatgtta tcaatacaca aaatgtgcgg tcgattaata aaaaaaccag agaagtcacg
     9121 ttggcaaacg gcgagaccat cccagcctct atccgtggat taaagaaatt gatttcataa
     9181 aaagaaccga atagtttgga ccgactaaat gaccagccga tccaaaccct tcggttcttt
     9241 tttgcctatc agtgcaaaag catttcctgt gcaatttcac gtccatccat tgtatctgga
     9301 taaaatgttg gccaattctc catttctttc agcaatgctt cttgactttc atttcctgca
     9361 tataaatgaa aatgttcgga gtcagccggt gcaatcgtgt gatcactgaa ttgaatatat
     9421 ctaaaagcgc cactttcctt atcttctgct tcgaatagat accttacgcc acggtttcct
     9481 gcctcgtagg tcaagatttc tttccctaca taacgataat ttgaacgttt cgttgttcca
     9541 tccttaaacg taaatgccat ctctttctca ctgatcttga ttgaagcaat atctgtttga
     9601 tagcccttcg tataatattc tttatattct tgtgctgtct tatcctgatt caatttttct
     9661 ttatattcaa aaacaggatc aaatgtccca tcttctatat acgtatctac tgtttgccaa
     9721 gttcctgccc aatctgaaag agagcgatct tgtacatcct cgtcttcgaa ataaccgtta
     9781 tagacgctct tttctttttt gggggtcaaa gtatcttcta acgggttttc tgtctctgtc
     9841 gtttttttca acgcttcgag gtttgcttcc atgatagaaa gatagttttc tccattttct
     9901 atttgttcat tcgtcaatcc ttccaaagga ttcaatactt ctaaagatac acctgcttca
     9961 tttgctaacg ttctggcaat actatctttg gcgttttctt caaaataaat atagttgatt
    10021 ccatgttctt taacaaagtg tttcagttca gcgattctcg ccgcagacgg ttcttcacta
    10081 gaagaaagac cagcaatcga tacttgattc aatccatatt ctaatgccag atagttgaat
    10141 gcagcatgtt gcgtgataaa agatctttgt tttgcttgct taaaggtttc ttgataaagc
    10201 tgatggagtg cttgaagttt ctcggtatag gctgctgcat tttttgtcca tacttcctgt
    10261 ttttctgggt aagcttcaat cagctgatca cgtatgcttg ctacctgctt gattgctaaa
    10321 cttggtgcta gccatacatg tgggtctagc tcgtgtgtat ggccttcttc cccgtgttca
    10381 tggtcatgat catgtccttc ttcacttccc ggaagtaaaa gcataccttc tgtcgctttt
    10441 atcgtatgga cattgccttc ttgtaatgtt ttttggattg cggggaccca tgtttccatg
    10501 ttttcatcat tataaacaaa gatatctgta tcttggatct ttgccatgtc tttagcagaa
    10561 ggttcataat cgtgggaatc agtccctgct ggaatcaata actctacttc tccttcatcc
    10621 ccgacgattg cttttgtaaa atcatacatg ggataaaagg tcgtcatcac gctcaatttt
    10681 tctttactgt cctcggcttt cccacttgcc ccgcaagcag ttaacatacc tgctaaaacc
    10741 agcatggcca atatacctat caatcgtttc atttatgaca ctcctcttct catcttcttt
    10801 catttgaatg ttccgattcg ccttttgtaa accgtaatag ttacgtttta gcaacaggat
    10861 ttaatttatc atatggttaa aataactgtc aagcgttttc ttattttaat tgatgagaaa
    10921 gtttatgtga atgattgacg atatttttgc aatcaacctc tatcattttc cttttatacc
    10981 cgcttttgtc tataatgaag ttatagataa aggaggaaaa agagtgaaaa aagaaacaga
    11041 tacaacgaat cgtttgatcc gctttttagg aggaaaaacc tcttactatg ttttaggaat
    11101 agttatctta cttgcgctga tcatttattt tttccatcag atctcgttta tattcaagcc
    11161 tgtcggaatc attatcgcta ctatcttgcc cccattattg tttgcactga tcttgtatta
    11221 tcttttggaa ccagtcatca aacgtttgag caagcacctt tcgaggaact tgtcagtcat
    11281 tatcgtttat gtgattattt tatttttatt ggggcttgga agcgtatggc ttgttccttt
    11341 tcttgaagaa caggcaaaga cattgatcag tagtttgcca gatttgtttc aagattttca
    11401 agagagtctc cgacaatttt tggagaaaac accttttgcc gattcattta atcaattttt
    11461 cgcttctatt gacgacgtga cgtctgatat agctggattt attggaaatt attgggaaag
    11521 tggcgcacaa cggatcagca acattttttc aactgttaca gctattttta ttactttatt
    11581 tactggtcca attgtcgctt ttttcctttt gagagatcct caaaaatttt atcagtcagt
    11641 tttagcgatt gtcccaccag catttcgtac agattttaaa aatttgacaa agattgctaa
    11701 tcaacaattg ggttcatttc taaaggggca aatcattgct tcatttatcc ttggagcagt
    11761 ctactgggtt tgtttcttac tgatcggatt agaatttgct agtgttttag ctatttctgc
    11821 tgggttgtta tgtatcattc catatattgg gccgtttatc gccttctttc caggtttgat
    11881 cattgcattc caagactcta cttttatggc tgtcaaattt gtcattgtct ggtttgccgt
    11941 acaacttctc cacggtgact tggtgattcc tcgagtaatg ggtgatcgtt tacagatcca
    12001 tccgattacg atcttaatcg ttttacttgt catgggtgat ctaatgggaa ttgtgggcgt
    12061 tatttttggt attcctatct atacgttagt caaattactc gttactttta ttttccgtaa
    12121 attcaaacaa cgctacaata aattttatgg cgataaagga gagtatcaaa actcttcatt
    12181 ttctgaagaa gattatttta aataaaacac aaaacgtcga tcttacgaga atagctcgta
    12241 ggaacgacgt tttgtgtttt tgttttaatt tttcagcaaa gcaattgcct ggtatggttt
    12301 tagctccaga ctttgatgaa gaacagtatc ggaataatta tcgataagaa tattggcttc
    12361 tgtgaatcct tctgggatgg cgatccttgt atctttatca tagaaatttg ttaatacaag
    12421 cagctgctgt ccttcaaatt ggcgaagaaa gccataaact tccggatgat ttgcttcata
    12481 agcatgataa tctccttgtg caacgattgg atatgttttt ctcagccgaa tcaatttctt
    12541 gtaataggac aaaatagaat cttcagaaga tagttctttt tctacattaa tctcttctag
    12601 cttgcctcca acttttaacc aaggtttccc tgtagtaaat cctgcttgtt ctgtgtctga
    12661 ccattgcatg ggtgttcgag agttatcgcg agattttgct ttgatccgac gaaacgcttc
    12721 tttttctgtg aatccttgtt caaggagtaa cttataagca ttcaaacttt ctacgtcaat
    12781 atagtcggaa atcgatgaat agtctgggtc gatcatgccg atttcttctc ccatataaat
    12841 atagggcgtc ccacggttca aatggatcat ggttgctagc atctctgctc cttgttttcg
    12901 atactgatcg atagctacaa atcgattgag cgcacgtggc tgatcatgat tattaaaaaa
    12961 tagagcattc cagccgttac cctcactcat cttttctccc catgtatgaa atagtcgctt
    13021 taattctgca aaatcaaatg gaggagtaga ccatttattc ccattttcat aatcgacttt
    13081 caaatgatgg aaattaaaga ccattgataa ttcttctcta ctaggttctg tatacaagat
    13141 acaattttcg atcgtcgtag agctcatttc accgactgtg atgatagtgg aatccgtccc
    13201 aaacgttgct tggttcaatt cttttagata cgtatgggtg attggtttgt ccgtatattc
    13261 aggtttgcca tcattttctt tattattttt caattgttcg tctttaccga taacattgat
    13321 aacatcgaaa cggaatcctt ttaccccttt ttccatccaa aaacgaacaa ctttgaatag
    13381 ttcctctctt actgcttggt tccgccagtt caagtcagct tgtgtccgat catataagtg
    13441 caaataatat ttccccgtat ctccaaatgg cgaccaagca cttccaccaa attttgatac
    13501 ccaatccgtt ggctcttctc ttaaaatgaa gaagtcctga taatgctggt cccctgctag
    13561 tgcttttttg aaccattcat gttctgtcga aacgtgattc agcaccatat cgagcatgat
    13621 ttctatccgg tatttctttg cttcggctat tagctgttcg aattcctcca tcgtaccaaa
    13681 gataggatcg atggctgtgt aatcagagat atcataaccg ttgtcttttt gagggcttgg
    13741 ataaataggg tttagccaaa tcatgtcgat gcctagatca ttcagatacg ggagtttttc
    13801 tttgattcct tttaaatctc caattccatc accattactg tctttgtaag atttgggata
    13861 gatttggtaa atgactttgt ctttaaatgt catggatatt tacctccatt caactgattt
    13921 attacctacc aaaacttata tatacaagtt atcatttatt attataactg aaccgaaagc
    13981 gtttgtaaaa tttatcttta tacattctaa gaggtctgtt gcccaataaa aaacaagaga
    14041 tcagacatgt atttgtctat cctcttgttt aaaatagcaa aatattcttg tttcttctat
    14101 aaatggtgct caaaagcttt tatctacggc aatcagtctt ctcaaatgta aaacatgagg
    14161 agctaatttt atttgagtat tctttttgct cgatgcagga cagctttttc acaacctctg
    14221 attcacggtg ttcaattagc agctgaccct cgttattagc tcaaaacgct gatttcacaa
    14281 cctcttcctt aagcggtgtt taaaagcttg catctgcggt aatacgccca atcaaacgaa
    14341 aaatatgaga agctattttt gtttggctgt tcttattact tgatgcagga cagctttttc
    14401 acaacctctg tttttattca ttcgatttca acgcatcaat catatcgata tgctttaatt
    14461 tgaaatgggt aacgatcatc acaataaccg tgaatacgat agttaatcct gctgataaga
    14521 cataagccca ccatgtgatc actaatggaa agatgacatt ctccatagac gcttgttgca
    14581 agatgaagtc agtcaatata tatcctacac caaatcctcc gatgattcct aaaagagtaa
    14641 agatgatatt ttctcgaaca atgtacatcg tgacttcttt atcaaaaaat ccaaggactt
    14701 ttattgtaga taattcgcgt acacgctcgg aaatattgat attagttaga ttatacaaga
    14761 cgataaaagc cagcagtcct gataatacga caaagattaa gacaaccgag tctaagttcg
    14821 ccattgattc ttcttgcgtt tcgatttgtt tggacatgaa agacgtattt gtcacagcgt
    14881 ctgtggcaag cagtttctcg gaaagcattt cttcttcttt tgttgtcatc ttatctgtac
    14941 gcagcaaaaa tgcattatcc atcatcgatt gatcagtaac tgaagcataa tattcagggg
    15001 acatataaat aaagctacct agatagtttt tcacgatttc ttttacttcg actttccatt
    15061 ctttttgatc ttcatcataa agcgaaatat gatctcctgt agataagtca aatagttctg
    15121 ctgctttttg cgtaatagcg attcctgttt taggcaagga gcgtgggttc tcttcaagat
    15181 cagttaaatg gataaaacga tccaaatcag ctgttttact tggtacgatc actgtcagac
    15241 tttgcgaagc atgtcctttt tttctcaatt ctacggtttg agtatgaata gctaggatct
    15301 catttacttt ttcttgcttc tgcagcatat ccgctgcttt ttgacgacta tcttccgtgg
    15361 atgaatccaa tgtaacgatt gcttgatagt caatgatcgg tccaaattgt ttggctgaga
    15421 cagaacttag agaatctttc aatcccacgc ctgcaaccat cagcccggca catccagcaa
    15481 ttccgataat cgccataagc attcttgact tatatcggaa gagatttcgg taactgattt
    15541 tttgattaaa gctaaggcgt gaccacagcg gagtgatgta ttcaagaaaa atgcgtttcc
    15601 ctggcttcgg tgctttcggt tgaagcaatg cagcaggccg ttctcttaaa tctttgaata
    15661 aaacgatcat tgcagcgcca aatgcggcaa tgaagaatgc ggcagctgct tgtaaaatcg
    15721 gcggccagtc ataaaaaata agcgcactcc caatatcata ccgctcgtta gacagctcaa
    15781 aaatgatcct tggcaaaaga ttcgttccta atatcgttcc taaaacaatg cctatccctg
    15841 aggctagact tgcatagatt gcatattttt ttgcgatttc aactttcgta taacctaacg
    15901 cctttaatgt cccgatttcc ctgcggtttt cttctaccat acgtgtcatt gtcgtaaagg
    15961 tgatcaatgc agcaataaag aaaaagaaaa caggaaaaac attggcaatc gcagcgatac
    16021 gatcagacag atcaccaaat tcttgaaaac cgacattttc tgaacgttca ttgtataggt
    16081 atgatggttt agttaaattt tcaatttttt ctgcatttcg attcaattct tcctctgcag
    16141 aggccagttc acgttctttc tcaactaatt gcctttcttg ttcagcaagt tgagcttctt
    16201 gttgagataa tgtttgaaca gcgattgttc gttgcgcttc aggaagctgc tggatagttg
    16261 ctgactgttg cgctaactgt tttttagcat cttctaaccg tgttcttgct tgcgttgttt
    16321 gtgctttgcc atccgctatt ttttgctgtg ccggttctaa ttcttcgttg gccgattttt
    16381 tcagctcttc taagcgttct tcgggtcgat cttgtaaata cttttctgcc ttttcttgat
    16441 tttgctccat tttatctttg taagctttgc tgtacgtatt caaattcttt gcgtttgtaa
    16501 aatcaagata taagacagaa taaatatctg ttttaaaaac ggattcaggt aaatagacaa
    16561 aataatcgac gctaccattt ccgacgttcg cgtatcctct ctccaaattg ttgatgaaca
    16621 atggagaacg gacaaagccc gtgatggtat actctttttc tttcaattca tctgaatcaa
    16681 tctgataaat atcaccgatt ttataccctt catcttccgc tttttcatcc aatacaagct
    16741 ggtttggttt ttccgggagt tttccatcag ttaattgtag cttgttctgc ttgttatttt
    16801 tgttataaga aaatatttga atcacttcgt ttttgccagt attggaataa tagatctgtt
    16861 ttcctgtttc tacttttgca tctgggatct tttctgcttg agcaatatct ttttgtgtca
    16921 agccaccagt agaaacgatt tgcaaatcag aaagtcctgc tgtaccaaca tagtgatcca
    16981 tcgttttttg catgacaggt ccagcagttt tgacacctac aaataaaagt gttcctagca
    17041 tgacaataag gatgattgca atgaatcgtc ctttagattg ccgaatttcg cgaaaacctg
    17101 ctttaagata tgttcttgtt ttcataaaat ccccctacca tacaatctca tcaatggatg
    17161 aaggatgatc atttaattca acagacctta ctttagcatc attaatgtga atgactctat
    17221 cagcgatggg cgctaaagca gaattatgtg taatgatcaa aacggtattg ccgtgttgtc
    17281 tgctagcgtt ttgcaataat ttcaatactt gcttgcccgt ttcaaaatcc agcgcgcctg
    17341 tcggttcatc acataaaaga agtttgggat ttttagctag tgcacgagca attgaaaccc
    17401 tctgctgttc gcctcctgat aattgggacg gaaagttatt caatcgatga gctaggccca
    17461 cttgcctcaa tacctctaca ggatcaagcg catcaggaga aacttccgtt gccaattcca
    17521 cattttcttt tgctgttaga ttaggaacga gattgtagaa ttggaaaacg aaccctacat
    17581 ctgttcgtcg gtaagcagta agctgtttat ctgaaaattg cgcaatatct gtgtcatcaa
    17641 taatgatctg accttcatca ggtgtatcca tcccacctaa aatattaaga atcgttgact
    17701 ttcctgctcc acttggacca agtataatca ctaattcgcc tttttcaatt gaaaaagaaa
    17761 tatcgtcatt tgctgtaatg gtcgtttctc ccattttgta tcgtttgtat tcatttttta
    17821 cttcgacata cttcactctg attcctccat tttcacgata tttctcgcta tatctatgca
    17881 tagaatgcgt tttctcacac ttatttattt taccactgaa tttctctaga ctaaagaaag
    17941 gaatccttta tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
    18001 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
    18061 aaactgcttt tgtcgcgatc gacatgcaaa aagggatcgt aaataacggt acactctctc
    18121 cacacactgc acaatctatt ctgtcaaaca atgatttgtt agtaagaaca ttgaaacgta
    18181 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
    18241 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgac ttgttgttga
    18301 aggattcctt gaaagataca aacaatatgt tgactatcac aaaatacaat ccaagtgcct
    18361 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
    18421 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttc caacatgggt
    18481 atcatgtcgt acttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
    18541 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
    18601 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
    18661 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
    18721 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
    18781 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
    18841 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
    18901 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
    18961 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
    19021 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
    19081 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
    19141 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
    19201 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggtcatcaaa cgatcgccct
    19261 tgtctatcct gaaaatccgc cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
    19321 tcttgatgtt taccaaatct ctcacgacca actgatcctc ttacctacac agttctcaaa
    19381 acaaggtggc taccaaatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    19441 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaatctg gaaaaagcat
    19501 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    19561 gaaactgaca acgatcgcac aaccgatctt cgaattggga caaacatctg ccaaattatt
    19621 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    19681 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    19741 gaactattaa aacgttttac taaaacgttt taaaaaataa tttttcccat caaaataaat
    19801 gagatagttg gaggtttgta aaatgaacac ggttacagta attggaagta ttaatatgga
    19861 cactaccctt cgtttaacca acatgcccaa gccaggcgaa acgatgcatg cgcatgagat
    19921 ttttcacgct ggcggaggta aaggcgccaa ccaagcagtg gcagccaaac gttcaggcgc
    19981 acggacatct tttattggag gcgttggggc tgacagcgaa ggacaacagt tattagatct
    20041 actgacaaaa gaaaacatcg atacatctgg gatagctgaa atccaaggcg ccacaaccgg
    20101 acaagcaatg attatggtag acgcagcagg agaaaatagt atcttgatcc atgcaggggc
    20161 aaataatgct ttccatgaac aagaagtcct aaaaaacaag caactgatta caaacagtga
    20221 ctttattatt gctcagtttg aaagtagctt agatgcgacg atcctagctt tttctattgc
    20281 aaaagacgca ggaaaaacaa cgatcctgaa tcctgcacca gctcgtgaaa cgattcctac
    20341 tgaactactt gaaaagacag atatcattat accaaatgaa acagaaacag aaatcattac
    20401 aggaatcaga gtgacagatc ataacagttt ggtcgcagca gccgaaaaac tccatgaatt
    20461 aggtattgga acagtaatca ttacactagg cagtgccggc gctttctatc atacagaaaa
    20521 agaacacggt atcgtccctg cttttaaagt ggatgccgtt gacacaacag ccgcaggtga
    20581 tacatttatt ggcgcattaa gcagcacact tcagccagac cttagtaact tgaaagaagc
    20641 gatcctttat ggaaatctag cctcttctgt tgctgtgcag tcttatggtg cgcaaccttc
    20701 tatcccttac cgcgaagctt tagaacaatc agcagtctaa aattaacaat gagaggaaga
    20761 aacaaacatg aaaaaaggaa aagtaatcaa ttcagatatt tcacgagtaa tcgcacaaat
    20821 ggggcacttt gataaattaa gtatcgggga tgcaggcatg cctgtcccct ttggtactga
    20881 aaaaatagat ttggctgtct caaacggtct tccaagcttt ttatctgttt tagaaaacgt
    20941 tttagaagaa cttgaggtac aatcgattga attggcggaa gaaataaaga caatgaatcc
    21001 ggaaatattg gaaaaaatca aattattgtt accagataca ccgattagct ttatcccaca
    21061 tgaagaaatg aaaaaagagt tgaataactg tcatgcattt attcgtacag gcgaaatgac
    21121 accttacgcc aatattatat tagtgagtgg agtggtcttc taatggaaac gacaatgaat
    21181 cctgaacaga tggtactgta cgagcagatt catatgctga ttgaacagtt gggaggaaaa
    21241 gacaatattg attctgtcct tccatgtgcg actcggcttc gtatcaaatt gaaaaaacag
    21301 ccagtagcca atgcagaaaa attaaaacag caaatccctt ccattaagta tttcatttta
    21361 ctcgatagat atgcagaaat aatcatggat cagggattgg ctcctgctta caaggaattg
    21421 acgcgtcaaa tcgatcattc agctccgaaa gaaaagctaa atccgatcga aaaactattt
    21481 ggtttgatat cggaaatctt tacgccgatc attccgctgt ttgccggttc tggtatatta
    21541 aaaggtctgg ttgttttagc gactacgatt ggctggctta gtgaaacaag cggaacttat
    21601 catattttgt ctgctgcgtc gaatgccgtc ttttattttc tgccaatctt tcttgctttt
    21661 tctgccgcca aaacgtttaa agtgaatgga tatattgcgg ctgtcattgc tgcagcactg
    21721 attgaaccga atctgacctc tttactttca gacagtggca aagcagctac tgattttctg
    21781 gggattcctg tagtactgat gcagtatacg tctactgtca tgccggcgat tttaggtatt
    21841 ttcttttatt cttttgttga aaaattcctc aaaaaatata ttcgcgataa tatgcaatta
    21901 gtatttgtac ctttactttc attagttatc acggttcctt tgacgttaat ggtattcggg
    21961 cctgcgggtg tttaccttgg cgaaggttta gctgccgcaa ttacttggat gatggatatt
    22021 aacggaccga tcagcggcgc aatcatcgct ggtggttgga atattttggt aatatttggt
    22081 atccaatggg ctgtaaaccc tgtcatgatc agtaatatct ctgcttatgg gttcgaccga
    22141 atcgttccgt tgacaggagc agccaatttt ggaatggctg gtgcagcttt aggtgtattt
    22201 ctacgaagta aacgttcaaa aactcgttcg atctccggtt ctgcttttgc ttcgatttta
    22261 cttgcgggag tgaccgaacc aactgtttac ggaattgcga tcccattgaa aaaaccattt
    22321 gttgctgctt gcattggtgc agctgctggt ggagctgtaa tgggatttgc ccaagtgaaa
    22381 gcgatcgctt ttgtatttgg tagtctgacc acattgcctg cattcatttc cggtactttt
    22441 ttctggtact tagcaggact ggctgttagt ctagtcgtag caatgattac tactctcgtt
    22501 agcgggtttg atgaagacct aatgtcttat gaatagttaa tgaaaaagac tgaagtatga
    22561 gatgtttatc acatatttca gtctttttct ttttaggatc attattttta taaatttttg
    22621 tgatactttc caaactttgg tataatttac atatgcataa ccatttttat ccccacttgt
    22681 ttatccccaa acaaaggtgg tctatgcaat aaaatgaacg tcaaagaaaa acgaatgaat
    22741 gctcgttgct tatttacaaa ttgttttatt tagaatacac agacagattt atgaaaagaa
    22801 aaagcttttc gacacgatta tctgttccaa aaaatgtcta atgaatgctg aataagacag
    22861 aaaatcaatg aaaggaaaag gactagttta ttatgaaaaa taaaaaaacg atacgtttgc
    22921 tagctgctac ttggctgatt gctccgtccg ttttgtcgac caataccgta tttgccgtag
    22981 ataatcaaac agcagatcaa acagtcacaa cagatacgac tacatccagc tcagctacta
    23041 gtgaaacaac ggattctttt acagaagacc caactggaac aactaactca acaacaggat
    23101 cgaccgagcc aactgaaatt gatccggctg atccatcaga aaatgaaaag ccagccgaga
    23161 catattccct tacaattgct ccttctttga ttggtaagat ccaactgact gcttcgacag
    23221 cggattcccc tgaacagcaa cctttggata ttacggaaag tggaacggta gaaggtttat
    23281 tagcggaaac gaaggtaact tacactatta cgccagctga aggtgaacaa attgcttcag
    23341 tcgctgttaa tggtgccaaa gaagtaaatt atgatgcaaa gacctttaca gtaggtacga
    23401 acaatttatc tattactgca gaatttgaac ctgtaccgga tacttcaaca gagaaaccaa
    23461 ctgatcctag tgaagaagtc cctagtacac cagatccgga aacgccaaac aaaccagatg
    23521 tgggtaataa caacaataac aacaataata atggttcaaa taattcaaac aataataaca
    23581 ataattcgaa caacgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg
    23641 gaaatactca gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca
    23701 atgcttcatc tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt
    23761 cttctgattt tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg
    23821 tccaacaagc aatcgttcga gaagcattcg agcatcttgg aaaaccatat gtatggggag
    23881 ccaaaggtcc aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa
    23941 ccggtcatta tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag
    24001 taagtcaggc ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc
    24061 atgtaggtat ctatattggg aacggacaat ttatccatgc gccccaacca ggagataaag
    24121 ttcgagtaac ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag
    24181 gtttacctgc tacttcaggc tcgctaactg gttcatctat tttagatgga ttagatggct
    24241 cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt gcagatgatg
    24301 cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct caagctatcc
    24361 ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac ctttttggta
    24421 tcaaaggatc atataatgga agcagcgtga ccttcaatac acatgaacaa gatagttccg
    24481 gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag gaaagtttgg
    24541 aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc tacaaaccaa
    24601 cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa ggacgttatg
    24661 ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac gacctgacaa
    24721 aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgcc gaaatcctca
    24781 atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg ccaatcacag
    24841 gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc cgtgtcgctt
    24901 caatctggga tttgtggaac aattttacgg caagacagat accagaaaca actactcgta
    24961 cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa tcattctggc
    25021 ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact gtcacactcc
    25081 ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa ctcgcgtact
    25141 tgattaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc tggcagttct
    25201 tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat ttccggtcca
    25261 tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt tacttggatc
    25321 gatacttttt catctaggct tgtcaattcc gcagcattta gtaaaccaga ttgttttttc
    25381 aacaaaaatg gatgatcgat accgcccact atcattgatt ggcgaaaatc actggcaaag
    25441 acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac tgaaactact
    25501 tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt caaccaaagt
    25561 atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac atgatttgta
    25621 ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca tgtatcatcg
    25681 ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt ttccatgtct
    25741 gggctagtct gatagaaaat aaccgatgct tcgttttcac cattgatgac tgtataatgc
    25801 cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt gtccgggtct
    25861 gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtgat ccgtccagcc
    25921 atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt tgcttcatca
    25981 aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg aacaacccgt
    26041 gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatagt aatcaagtga
    26101 tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc ttttctttca
    26161 tttgcactga ttccagtata catagaaacc gttttcttaa cagcttattc cgtgagtaac
    26221 gagaaaaaat ttactatatt ctaataaaag accgcgaaaa aaggttgtga caaaagtatt
    26281 gtcacaacct ctttttccga acaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    26341 caatcaaacg aaaaatatga ggagctattt ttgcttggct gttcttatta cttgatgcag
    26401 gacagctttt tcacaatctc tttttcaata cgattccagt atataaccat gtcgtcagta
    26461 catagtagat gaaatacaat acgaagaaga tcgtaaacgg aacccaaata tctttgtatg
    26521 gattttgcat gaatagacca aacatttgta atccgaataa gacatggact acacctaaga
    26581 ttcctggtgc taagaataac acgcctactt ctctacggat cgattgttta agcaacccgc
    26641 gtcgtgttcc tatcttttca agcatttcat aacgagcaat gtcactcttg cttcctgaca
    26701 agatcttgaa catcaaacag ctagcaagca tggtcaagaa agctaaacct aggaagaatc
    26761 ccataaattc aaaaccagag aatagaccat tatacacttc atacatttca tatttctggt
    26821 tgctgtactc tactttttgc aaagatggat tgttcatttt attttgagca acaagtgttt
    26881 cgattggttt caggtttgct tggaagtctt ttacctgaac tagctgtaaa gtggtttgtg
    26941 gcaagttcag ttgatcaaat tctgattgac tgactacctt gatttctttt tcttgctgtt
    27001 ccggcaattc atagcttcga agttcctctt gtacagtcag ttcattcatt ttttcagcag
    27061 gaactttttc tttcgtgatc ttcggcggtg ttccatcttc atgttttatc atgatcaatg
    27121 gttgagccgc aaattcactt gcattatagt aaatcgttcc cgcatcttct ttttgagtat
    27181 aagaagcaga aagtgttggt gaaagttctt tgattttttg ctgatcttct gcccgaggat
    27241 tattcatcac tagatcatat ccagaattgt tttctgtcaa gtctgcaatt tcatttttaa
    27301 aacctaatcc taccgtcaaa gcgcccagtg ccaaggcaaa cagcatagcg accattgaga
    27361 gcatttgtgt atattcacga atacgaaagt ttaactgtga caatgtgaaa ttgttcaatt
    27421 ttttcaatga aatctgttct gatttcttta gtaatgataa aaagaaaatg atcacagaat
    27481 ggaaaatgaa ataagttcct aatacgatcg taactaaggc tactcctata ccaaagactt
    27541 gataggtccc cacgtgtttc atcatatagt aaccgcagcc tagaaaagca attcccagta
    27601 ctacttcaag gaaaaacaag aatttatgct gcttgattct tgttggtgtc tgatccgcac
    27661 gaatcaatgc taaaatcggt tttttgacaa tcgaaaaagc attgacaaca gcagctaata
    27721 aaaacaaaac agcaaaaaac aaaacagtga tcaacaaccc gtgaatacta aaaggtgtaa
    27781 aatgcgaaat attgagatct aactgatcca ctaataatcg gtggacgata cttgtcaatc
    27841 ctacaccgac agctgaaccc actaaagtag ctgcgatccc tactaagaat gtttcagaaa
    27901 aaatcatctg tgcgattttt ctgccttttg ctcctagcat cataaacatt gcatagtctt
    27961 tttgccgcat cgtcatcaaa aaggaatttg catataaaat ataaacgaat gtgatgatcc
    28021 ctagtaaaac agaaccgaat cggaaaatga taactgtagc cgcaacgatc gtattacttt
    28081 tcaaaaaagc ttcgttgctt gccattgatt caaacatgta aaaaatagca gatgccataa
    28141 ctaatcctga gaataacacc aagtagtctc ttaaacggct cttgatccca gtaagtgata
    28201 atttccaaag catagtcaat ctccccctac tgttccaagt ttccaagaat agatagtatt
    28261 tctcgataga aggaatcacg gctttgatct ccacgtttca cttcttgatg gataccgcca
    28321 tccttgatga ataaaatacg ttggcaataa cttgcagaaa acggatcatg cgtcacaaga
    28381 agaatcgata catgatcttt tgtattcaat tcgtccatcg tatctaaaag atcccgggca
    28441 cttttggaat ccagcgcacc tgtaggctca tcccctaata gaatcgttgg ttgtgtgatc
    28501 aatgcacgtg cggcggcgac ccgttgtttt tgtccaccgg aaatctcaga aggatagctt
    28561 tccaaaatat gagagatgga taaacgttct gcaatttttt ggacacgttg cttgatttct
    28621 tttggtctga cgccttgtaa agaaagagga acagcgatat tttcttgtgc tgtcaagctt
    28681 tctaataaat taaagtcttg gaaaataaag ccaatctcat tcgcacgaaa atctgctagt
    28741 tgatttcctt ttaatgtggt cacatctttt tgattgattc ggatatgccc atctgttggt
    28801 ttatctaatg tggataaaat attcaaaagt gtcgatttac cagaaccaga agcgcccatg
    28861 atcccaataa attcgccttt ttctacatcg aagctgatgc cgcttaatgc ttgcgtcttt
    28921 ttctcattgt tttttccgta tacttttgtt acattctgta cttcgactat tttgttcatt
    28981 tctttcccta ccctttaatt agtttttcca gtgctgtttt cggcacgtct tttttttcga
    29041 cttcttcata cgaagtgaca cctttatttt tgttccaggt gatttttaaa tatgctcctt
    29101 tacgaagcgg tcgagtcaat tgtcccttga agtccagttc tttttcctta ccctctttcg
    29161 tatagccagg caggatatat ctatagttga ctgtttcttg tccagagtct gattttgaaa
    29221 ctgtttttgt accatctgtc gtaatttgta catagtattc ttctccaccc ataactacgt
    29281 aatctgcgat tttcacccct gcaagtacca caacgatgaa aacaagtaga ccgatcagaa
    29341 gttttttcac tttttcccct tctctcttac tttatatact gattatagaa aaaagcgaac
    29401 aattctgcct tcgaatcaac tatcattctt ctttcaattt tgtcattttc tttaaaacaa
    29461 ctaaaaaaag agttcaagac aataatccgt cctaaactcc ttcttcctat cttacaaaaa
    29521 gtatgtcact tacggtcttt catgtcgcct gtattgataa caggttgcgt tcctttgtct
    29581 gtaatgattg agacaagtaa attattgatc aattggactt tccgatcatc ggtgaaattg
    29641 atttcttgtc catcttcgat ttgctctaag gccatttgag tgattgtgac agctccttca
    29701 acaatggttt gtcgagcaga caaaatggct tttgcttgtt gacgttggag catggcacta
    29761 gcgatttctg ttgcataggc taaatggttc aagcgtgttt cgattacttc gacacctgct
    29821 actgctaaac gctcttgcag ttctttttgc aactcgtcag acactgccgt cgtgtttcct
    29881 cgaagcgtca aatcattctc attgaatgta tcatacgggt attggctagc gatatgccga
    29941 atggctgtct cactttggat ttctacaaaa tcttgataat acgctacatc gaataatgcc
    30001 tttgctgtat caacgacttt aaagactact actgctgaga tttcgatggg gtttccatcc
    30061 aaatcattga cttttaaaac agaactattg aaattccgaa ctttcaacga aacagtcatt
    30121 ttttgtgcca aaggtatcgt aaggaaaaag ccattttctc gaatcgtacc taaatattgt
    30181 ccaaaaaaca aaattacttt tgcttgatta ggactgacta cagttgccga acttaacaac
    30241 aataacgtga tcaaccaaag aaaaatactt aaaaaaagtg cccaaacttt cgcttgccac
    30301 atgcctaaat agaagaaaaa caatccaact aaagctaata cgatcaatcc gacaatccct
    30361 gcataaccat tgatataaaa tgctcttttt tctctcatgc tacttcctgc gctcctttat
    30421 ccttcaataa atatctaatg ttactttttt aatttttttt tcgaatacgc gccattacac
    30481 cttctagttc taactctttg tcttcagtga tgtaccaacc gcttgcctgt gtccctccgg
    30541 ctgcctgcgg taaataatcc aattttgttc gaacccagtg cttttcttct gtaaaatagt
    30601 cttctaccaa gacctctaga tccatccctg ctgaaagagg caaatcttcc cgaacagatt
    30661 taaagataaa tcgttccttc ttcccatcat acttcaatat tcctgtatcg ataatcggta
    30721 aagaataata atccagctct ttcttcaccg tcaataaatg tttgaaaact tcatggtaca
    30781 cttgctctaa ttggcgacct tctgaattgt tcgggtaatt aaccatcact ttttctttgt
    30841 ctggatcata gaaggtttgg cttagttgag tgatataagt atgtaatctt tccatttctg
    30901 tctttaactt ttctctttga ccttcttctg ccttcatata ctttttcacc ctgttttctt
    30961 tatagatatt ttctttctta ttttactata gttatgtgcg ttaagcgatg aaaaacagca
    31021 aagattggaa ggatctttgc tgttttttct taaaatatct agttgagtac aggatcaatc
    31081 tgtttcattg tcctcttgat ccgtcgtaat attgatcgat gtgtctttag cagtgatctt
    31141 tagctttttc tgtccttgtt ctaacaaagt atttgttatt ggctggtagt ctttttcaat
    31201 ataagattca ccagacgttt ggatagtata gccactgttt tccatctcat gcaaagataa
    31261 attaccaccg tttaggatga cttttccttc tttgctgatc aaactgtctt ctagatggaa
    31321 gttgctgttt tcacctgtga cgatcaagtt atccacattg gcctcatcta gtcgaagtga
    31381 gcctaattca tctttcgcag ttattgttcc tcctgtaaat tcattgaacg atacccagcc
    31441 atcatgtgtc ttagtattca cttttttgat tgatacttcg ttgatatcaa ttgcactatt
    31501 attcccttcg attgtcaatt cagataattc ataatctgca gggacaacaa tagtgatttg
    31561 tgcacttgtg tcttctaaac taatgattgc acctgttgat tttttgactg cattcacagt
    31621 caacacgcca tttttctcct ttatttctgg cttttctgtc ttatcataag tcgtttttat
    31681 ctcaaaatcc aatccgcgta tgaccgaaac tgtttgattt tctgcatgga tgacgatttt
    31741 gtttgcatcc ttgatttttt ttgtctcagt aaccatatcg atcaaccgag gaccattatc
    31801 ccaagtcaga ctcttttgag cgcctaatgt ataagttgca cctgctagga ggccaccagt
    31861 aagcatcaga ccaataccgg taatcaaaaa atgtttcaat ctcaatttat gtgtcctcct
    31921 tcaaacgccc gctgaatgca gatctctttt tgtaataaaa cgtcgtccca tccatttaac
    31981 aaagttcatc atgacataaa aaagcccttt tgttcctttc agcaacagtg gcgaaataaa
    32041 tactgcgctt cctgtggcga acagaccgac accgataaag aataatccac tcagaatcga
    32101 ttgtccaaac acacttattc ctgatatgat catgaatagg ccaacaccta agatactgat
    32161 acataaaaca tagatactaa aaatgagcgc tagtaaagca accaatagtc ccccaataac
    32221 caatataaac cccagtgcta gtggaatgag gacaggagaa gcaaataaac cgataatgat
    32281 taaccaaatc aggcgaattt gtctttttgg atttttcact aactcgtttt ctgcttgttc
    32341 ttcttctgaa aagtaactga tttttaattt taaggcaaac tgctttggtg taccatattc
    32401 atcaatgatc tgatctaatg tttttccaga atctaaaaaa tattcctcat aatactggat
    32461 caaatcaaaa cgatcgtctt caggaatttc ttgaagatga tccacaactt cattcaaata
    32521 ttcattcatt tgtgctctcc tccaataacc ggtctaaaga atctcgaaaa acaatccatt
    32581 catcaataat ttcttctaaa tgacgacttc ccattggtgt caacgagtaa tatcgacggt
    32641 ttctgccttc aaaaggttca tcataggtct caagatatcc ttcttttttc aatcttctca
    32701 aaaccggata tagtgtagat tcactgacag agagactttc ttgtacgatt tttgttaagg
    32761 catacccata catatcttcc ttgcttaatg ctgctaacac agtaccatca agaagaacag
    32821 ttggaatctg gatgtgcatt acgtcaccct ctattctcaa gtgtaatatt ttatttcaat
    32881 ctatattata caacatataa tatagtttca aaatcagttt gtcaaataca aaaaaactga
    32941 ctaaaagatt tgatcctttt tagtcagcaa aaaaatggct tctctttgat ttttattata
    33001 acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg atcattgcag
    33061 gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg gtagcgacca
    33121 tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc aatggataaa
    33181 taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt gctatcacag
    33241 aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca aggacagatt
    33301 tagaaatcaa aatcgtactg tctcctgtca ttccagaatc taaacttcca tagatccccg
    33361 taccacttgc acaaaataga acaatgaccg tcactaatgt actgataaat tcatcgtggg
    33421 tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc agcgcagccc
    33481 ccttatttat cccattacct aaatgaacaa tcagaccaat cgctgttcct atcactaaag
    33541 caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc atccccatcg
    33601 aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat ttatttccta
    33661 ctaatccgcc aattagtccg ccaagcaaaa cggaacctac attgatcaaa actccagttg
    33721 gcatcaaaaa aactcctctc tttttctacg aagcgaaaaa aatcagaaaa atttctgtaa
    33781 ttattgcttt cgctgcaaga aagattgtaa cagacagaga agattacacc aattcatttt
    33841 ttgtgttaaa gtattcttta ttggaataag taggaggaat atgatggata tccataaatt
    33901 cgaagttttt cttgatttag ccgaaacaat gaatttcacc aaaactgctg atcgacaatt
    33961 caccacccaa ggaaatattt ctaaacagat tctttcgtta gaaacagaat tagacgtcaa
    34021 attattcgaa cgcgcccata gaaaaatcga attgaccgaa gctggaagct tattgctccc
    34081 atatgtaaaa aatgtcgtag aacaatacca tgctatgcaa gaagtgcttg aaacctatac
    34141 gaaagacaac aatctttcat taaatatctt aacgataccg acaatgatca attataaagg
    34201 tttttccaaa atcaccgact ttttgaaacg ccatcctgaa tttacagtcc agttgaaaga
    34261 agtggaaagt gtcgaattaa cttacaatga cgccaatgaa catagtgaca cgatccactt
    34321 tgctagaagt ttccaggccc cgccgagttc gattgagtgg ttgcctactg aaacagacga
    34381 ttttgtagca gttctaccta aaaatcatcc tttagctagt aaaaagaaat tggatctaag
    34441 cgaactgaaa aaggagaact ttttgctact tggccccact acgaacttgt atcaacctgt
    34501 actagatctg tgtcgcgaag ctggatttga accaaaaatc tcttataaag gagcaagaat
    34561 cgacttgatc atcaatatga ttgcaaatga catggggatt gctattgtta tggaaaaaac
    34621 agtcaagaat ctattaaaag aaaatactgt gattctccct atttctccaa caacagagag
    34681 ttatcttgca tttactcgta aagtcggtga gcattctttg gcttcagata cattttggga
    34741 gtatttaaat caagaagaaa actaagccct acattttctg tccatctatc caccccaaaa
    34801 aagagagcga cgcatgactt tcgctctctt tcttacatcg tgccattgat tcaatgacac
    34861 atctaacgtt tctcgtattt caatagaaga tcagcgattt tatgccggaa gaacattaag
    34921 agcaattcgc taatcacaaa aaaacgtcta aaaatcccta aaaaccttca ctagaatttt
    34981 taggtatttt gttctttttt cctattattt gcttaaacgt attattgctg tcgtccctac
    35041 tccttcgata ctgctataat cgatacgtcc atcatgtagt tctagaattt ttttgaccag
    35101 tgaaagccct agtccattcc cagaaatctg acggcttgta tctccttgat aaaatttttc
    35161 aaacattcga tctcgggtct cttcattcat tcctacacct gaatcggtca cttccagtac
    35221 gatctctgtg gctgtctcaa ataatttgat atgaatcttt ccattgactt gattgtactt
    35281 tatggcgtta tccatgatat tcagccacac ttggtacaaa aactcttcat tacctgtata
    35341 gtttactgca gcaagttcga tatccaattc tagctgttct ttttcccatt taggctgcaa
    35401 aaaaacgatt acttctctga tttgttcatc taatcgatac tctttctttt ccagttggat
    35461 acgctggttt tccagtttgt ttaattttaa aatgttctct gttaattgtg aaagctgcgt
    35521 gatactttcg atgacccgat ataaaaatat ttggcgttct tcgtcagaaa gatttggggc
    35581 ttgtaacaat tgaacatatc cttggattgt tgctagtggt gttttgaatt catgggatac
    35641 actggacaca aagtcattcc tcagtgtctc gatactattg agttcttgga ccataacgtt
    35701 gaaatcttta tacagttgct gtacttctgc tactttctgc tgttcatcca ttcgaatcga
    35761 aaaatctccc gttgctacca gactcatatt tgtccctagc gcactgattg gatgcaggat
    35821 acgccgccca acaaaaatcg atattcctgt acccacgacc atactgaacc ccgcaaagac
    35881 gaataacggg tataacgttg gttttccagc cgattcagca ggcgagatag aatgtcgaga
    35941 caataagaaa ataaaaccaa gaaaacacag cagtgttact aagatcgaaa taaatacgat
    36001 actagcaaaa tagatccata attgtgaaaa tagcttcttt ttcatcttta aataaccgcc
    36061 ttatagccta gtccccgtac tgtgatgatt tgaaaatctt ctgtcttttc aaaacgggtc
    36121 cttaaacgtt tgatatgtac atcgatcgtt cgttcttctg tattcgtgtc taatccccaa
    36181 atatcgtcca tcaactgctg gcgagtgaaa atcttgtttg gataagacag taatttatac
    36241 agaagcagaa attctttttg aggcagaata aaagaatgct gattttgtac cactgtataa
    36301 gtatccagct ctaattttgt ttgcccaatc gtcaagatac tgctgtaatt gatttttgaa
    36361 cgtcgaagca atgcttccac acgcaggatc atttcattga catcgatggg ttttgtcata
    36421 taatcatctg cacctaattt gaatccacgt ttcttgtcct cgaaatcagc ctttgctgta
    36481 atcatcagaa ctggcatgtc gaatctggcg ttacgtaatg atcctaccaa gtcatagcca
    36541 tccatttcag gcatcatgac atctgtaatg atcaattcta tatgtgtcgc ttctagtttt
    36601 ttcagcgcat cctctccatt cagtgcggtg atgactgaaa agcccgctcg ttccaacaca
    36661 gaacggtaaa gcaactgtaa ttgcggatca tcttctacta ctaaaatacg attcattgat
    36721 ctccctctgt tttttgaaaa atttctagat tttttcttgt agttcatctt cagttaacat
    36781 ttatttcgta tactacgatc attgttctga tatacttatt agtataccga atatttcaat
    36841 acgggagaag ggtgattatg cttcaattaa aagatataaa aaaatattat aaagtcggcg
    36901 aaaccaccac caaagcattg gatggtgtgt ctgtcgcttt cagacaaaaa gaattcgtag
    36961 cgattttagg tcctagtggt tctggtaaaa cgactatgct gaatgtgatt ggtggactag
    37021 ataattatga ttccggtgat atggtaatca acggaaagtc tactaaagac tttaaggaca
    37081 gtgactggga tgcctaccga aacaactcta tcggtttcat ttttcaaagt tacaacttga
    37141 ttggtcatct aggcattatt gaaaacgttg agcttgggat gacattaagt ggtgtttcta
    37201 aagacgaaaa acgaaaacgg gcagaagatg cattgcaccg ggtcggcttg acagatcata
    37261 tgcataaaaa gccaaatcag ttatctggtg gacaaatgca gcgagtggcc atcgctcgag
    37321 cactagccaa tgacccagat atccttttgt gcgatgaacc aaccggagcg ttagatacag
    37381 aaacaagtat ccagatcatg gaattgatcc aagagttatc gaaagaaaaa ttagtgatca
    37441 tggtcacaca taatcctgaa ctggcccatc aatacgcaga tcggatcatt gaattttctg
    37501 atggtaagat cttaacagat tcccatcctc acatcgaacg gccaaaagat gatcaattta
    37561 acttacgcag aacaaagatg agtttttgga cagcgttgaa gctttccttc aacaatatcc
    37621 gaacaaaaaa aggacgtact ttcttgacct cctttgcttc cagtattggg atcatcggaa
    37681 tcgcgatcgt tctgtccctt tcttctggat tccaaaaaca aatcgataac acgcaagctg
    37741 aaacgatggc gaaatttccg attactattt ctaaggtaac gactaaccag actcgtgacg
    37801 atgccggatt gggtgcaagc aaagcggatt atccagacag caaaacgatt actgcaaaag
    37861 tcagcgaaga agataaagca cagcacacga ataagattga tcaaacatac gtcgattacg
    37921 taacagacat cgatcctaac ttaagtaata atatcggatt tacccgtact acaggaatca
    37981 atcttttacg tgatgtaaat ggcaaagtac agcctgtcag cttctcgaat caaaatcctg
    38041 atacggaatc tctttcttta tctagtacga tgtcagcaat gacaggtgtg ggagtgtcaa
    38101 gtttccctac acagcttgac actagcaaag aaaacttttt aaaagacaat tattctttac
    38161 ttgccggaag ttatccagcc tcagcaacag atgtcgtgct gatcgtagac ggcaataata
    38221 atactaatat caacgcattg aagaatcttg gatttgatgt gaaagaagac gaaaaacttg
    38281 attttgatga tatcgttggt acaacattca aattagtaaa caacaatact tactatacaa
    38341 aacttccaac tgggaatttt atccctaata cagattatga tgcgatgtat cagaatgctt
    38401 ctgatgaatt gaagatctcg gggatcctac gcgtgaaatc ttcttctaca atgaatctct
    38461 tatctccagg aatcgcttat agcgatcaat taacgactca aatcgtcaac gaaaacaaag
    38521 aatcagaaat cgtcaaagct caaagagaca gtgatgtaaa tgtcttaact actgaaaaag
    38581 ttgacgaaaa tgccaaacaa acattgctta gttatttagg tggagacagc ttgccttcaa
    38641 gtatcatgat ttatccgaac aattttgaag acaaagaaaa gatcttggat tatctagatg
    38701 attacaataa aggcaaatca gatgaagata aaatcattta tactgattta gctggaacga
    38761 tgactgaact gactggcgga ttgatggatg ctatcactta tgtccttatt gcttttgcag
    38821 ggatctcttt agtaacaagt atgatcatga tcagtatcat tacttataca tctgtgattg
    38881 aacggacaaa agaaatcggt gtactaaaag ctttaggtgc acgtaaaaaa gacatcactc
    38941 gcgtatttga cgcagaaacg tgtattttag gtatttcttc tgggatatta ggtgtgttta
    39001 ttgcatggct agctacattc cctatcaata gcatcttata caacatgacg gatctaaaaa
    39061 atgttgctca actaaatcct gtccatgcga ttattctagt gatcgtctct actatattga
    39121 cgatgcttgg cggtcatctt cctgcacgaa tggcagcgaa aaaagacgcc gctatcgctt
    39181 tgcgtgcaga ataaggttgt gacaaccaca tttagatttg agtcataaga gcgctgaaat
    39241 agtagaaaac agcaagaaac gaggaagtaa tatgttcctt gtttcgcgct gtttttttta
    39301 attatttttt ataattctgg tatacttttc tttcctttgt accacaactc ttctggcgta
    39361 tacctgtctt ttccatttat gaatactgtt ccatctgctt caatcgttac ttcagtccca
    39421 atccacatat tacgagaaag tgcgagaaga ttttttcttt ttcctcttcc tttttttcca
    39481 aattgcaagt catcggttat ctcttctgtc caatgagatt tcacgtattt tacttctccg
    39541 ttttcaaatg taactttcaa aatattcgct atattttcca cttttgctgc tttaggaaat
    39601 gactcctcca tttttttcac tttccttcct ccaacactaa gtatgcgctt tcttttattg
    39661 ataagtatat catagtttat tctattatca aaaaaacgca tccattccat tgtagaatac
    39721 tttaccgaac aagttgaatt gttttcctct ttttcacctt ttactataga taggtaaaga
    39781 caactaagga ggaatctctc atgacagatc aaccgtatga tttaagaaaa gtcatcgaag
    39841 aactaaaaac acttccaggt caatatcacg aaacagatat tgaaatcgat cctgaagcag
    39901 aattatctgg tgtttaccgt tatatcggtg ctggcggtac agtaaaacgt ccgactcaag
    39961 aaggaccagc tatgactttc aacaatatca aaggtttccc cggcacacgt gtgaacatcg
    40021 gaacaatggc tagtcgtaaa cgtgtaggtg aaattttgca ccatgattat cgaacacttg
    40081 gtcgtttctt gaaagacgct gtagaaaatc caatcaaacc tgtgaaagta gacaaagcag
    40141 acgctccagc acaagaagtc gttcatctag ctaccgatgc agacttcgat attcgcaaca
    40201 ttttgcctgc acctacaaat actcctgaag atgctggtcc atacatcaca atgggtgtag
    40261 tcttaggatc agatccagaa aaaacaatga gcgatgtaac gatccatcgt atggttctag
    40321 aagataaaga tacgatcggt atgtacatca tgcctggcgg acgccacatt ggtcacttcc
    40381 aaaaacaatt cgaagccttg aataaaccaa tgcctatcac tatcaatatt ggtttagatc
    40441 cagcaattgc cattggcacg actttcgaac cacctaccac tccacttggc tacaatgaac
    40501 tatggtgtgc aggagctttg cgtaacgaac ctattcaaat cgttgatggt atagcagtcg
    40561 atgaagtggg tattgctcgt gctgaattca tcattgaagc agaaatcatg ccaaatgaaa
    40621 caatgcaaga agatatcaac acgaactcag gaaaagcaat gccagaattt cctggctata
    40681 ctggggatgc taatccagct gtacaagtag tgaaagtcaa agcagttact caccgaaaag
    40741 acaatccaat catgcaaaca acgattggtc ccagtgaaga acacgtatct atggccggta
    40801 ttcctacaga agcaagtatt cttgccttgg tcgataaagc tattccagga aaagtagtaa
    40861 atgtctacaa tcctcctgca ggtggtggta aattgatgac catcatgcaa atccgtaaag
    40921 aaaacgaagc ggatgaaggt attcagcgtc aagcagctat tcttgcttta tcagccttca
    40981 aagaattgaa gacagtcatt ttagtcgatg aagatgttga tattttcgac atgaacgatg
    41041 tagtttggac tttcaacact cgtttccaag gagataaaga cgtcattgtg atcccaggaa
    41101 tgagaaacca tccattggac ccatcagaat taccagaata taatcctggc tctattcgtt
    41161 tccgtgggat gagtgcaaaa actatctttg acggtacagt tcctttcgat atgaaagaac
    41221 attttgtacg tgcaaaattc caagaagtaa aagattggga aaaatatttg aaataatgaa
    41281 tgatctgcaa ctgttttgac atcataaaaa ggagtgcgga aaatggaaca attggcacaa
    41341 cggaagaaaa aaatcgtgat tggtgtaagt ggcgcttcag gcaccattta tgccatcaac
    41401 ctagtgaaga aactaaaaga atatccgatg atagaaacac acggaatcat cagtccatgg
    41461 gcaaaacaga acttaaagct ggaatccgac ttatcactaa atgaattcga aagcctgttt
    41521 gattaccatt attcaaacaa agatttaggt gctgctgtcg ccagcggatc atttttgacc
    41581 gatggaatga tcatcgtacc agcaagtatg aaaacagtcg caggtatctc tgttggttta
    41641 ggcgacaatc ttattgcacg tgctgctgat gtaacaatga aggagcaacg aaagttagtc
    41701 attgttcctc gtgaaacgcc attaaatgcc attcacttgg aaaacttgac taaattagca
    41761 cgactaggtg tgcaaatcat accaccaatt cctgcttttt ataatcagcc tcaaacgatc
    41821 caagatcttg ttaatcacca tacaatgaaa cttttggatc aatttggaat tgtaaatgag
    41881 cattcaaaac gttggcaggg tgattaatat ggaaaaagaa acgatagttg aagtaaccaa
    41941 actaggtttc acgatccaag ctgctgttaa agtaatcggt aaagacttat tgatcacttt
    42001 aacaggcgga gatacaccac atatcggaac agtcactaca ttttctcgtg atacagagat
    42061 gcaaactgta cgtttcccta gccacgatgg tcggttccat aaagacgatg tcttagccga
    42121 aaaaatagct gtgatcatca aaactgccgt tccgggaagt tgtacgatta cttccggtat
    42181 ccatgttgac catatctcca atgcacaaat caacgcctct tttccgatgt cagaagagtt
    42241 aggttacaag attttagatt ggttggaaaa aatagatttc catatcaaag aaccagttta
    42301 ttataaagat ggtgaaaaac cattatgaaa gaagaggttg tgaaatcagc gttatgacct
    42361 gagtatgaga gaaaaaaatg gaaaaatagc ttctcatatt ttgccgtttt tgagctaata
    42421 ccgagggaca gctgattgaa cgccgtttat caagaggttg tgataaagcc gcttcgtttc
    42481 gagtagtaag aaaaattttg taaacatggg tttccacatt tttgcataaa tttgacttac
    42541 taccgagaaa ctggcttttg aacaccgttt atcaagaggt tgtgacacgt cttttgtcac
    42601 aacctctttt atttttgctt cactgtaact gctacaatat caccgatttc tttaccgatg
    42661 tttttacgaa tatcttttcg tagtccaagg atgtagcaga ctgaaccatc tggatttttc
    42721 acacccatat tcacgatact gccttcgtac ggcacattgt caaaagttgc ttggacttta
    42781 actcttccag caccgaattc ttttcttata tcatacggaa aaataatata tgccccgccc
    42841 ttgccgactt cggaagcata gatttttgat tcaaaagaat aaatttccct cattttattt
    42901 ctcctatttt ttacatctag tatagcatag caaaaaaaag aaggaactgt gacaaaagtc
    42961 ttgtcgcagt tccttttttc gaataaacgg tgttctcctg ccaactcctc ggtcacaagt
    43021 cacaatattc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcagagtt
    43081 aaacggcagg ctcacaacct cttccttaaa cggtgttcaa aagcttgcat ctacggcact
    43141 aaccccaatc agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga
    43201 tgcagagcag ctttttcaca acctcctaca cggtgttcaa aatattattc gccttttgct
    43261 tctttcacta attttgcgaa tgcttgttgt tggaattcag cagctaaggc tttcgtacgt
    43321 tgttcttgct ctgctttgct catttttgga ttttcagtca ttgcttttac cataccgtct
    43381 tgaacataag cttttgcttc actttccatc gtactttgct gtttcttaac taattcagct
    43441 aacttggctg cttggtccgt acttaattct acccatgtct ctgggatttt gaatgtattc
    43501 actcggctgt catattcgtg attgttatca gcaatattga accagaattt atacatgtcg
    43561 tttttgtatt cccaataagt agttttcgtc actttttcag catcgccgtc tgtcttttct
    43621 actttatttg tttcgttatc cgtaccagtt gcttttggtt tttcttgttt ttcatttgtt
    43681 cggtaaagat acactttttc ttttccattc cctaaagctt tatacaaaag catatctgct
    43741 ccttgggaat cagcactgga aacaattttt tctgtttttg tttccgttac tttttccatt
    43801 ccaaagtgat tggacaaatt agcgacgatc aaacttagtg aagcgacgaa gattagtccg
    43861 aaaatcaaag ataaaaaggt ttgccatgtt tttttagcga acacgttcgt taaagcaaaa
    43921 gcaagcaagc tgacaatcaa aactccaata atcatgcatt atcccccgct ttcgttagaa
    43981 caggttcttt tttcttcaat aaaaaggcaa taataaagcc aaggatccca aagatgatcg
    44041 ctacgataaa cgcggcatgg taacctgaca gtgtggcatt gattgcctga tctttataag
    44101 ctaatggtgt attatccaat acagatttgc ttgggatatt tgcttttgtc acattcgtca
    44161 acacactgat caaaatagct gtaccaatcg agcttgcgat ttgacggaat gtattattta
    44221 ccgctgtccc gtgactgatc aagttatttg gcaatgcatt cataccagaa gtcgttaccg
    44281 gcatcatgac catggagatc ccaaacatac ggatcgcata aaggatcacg atatataaaa
    44341 ctggagtcgt ttttgtcaag aaagcaaatg gtgcagttgc ggctgtcaaa atgaacatcc
    44401 ctgtgatggc taaacgtttc gcgccatatt tatcgaaaat cgcacccgta acaggcatca
    44461 tagcccccat gatcaatgca cccggcaaca acatcaagcc agaatggaat gctgattctc
    44521 cacgaatatt ttgaatatac aatggtacga ccatttctgc ccctaccatc gccatatttg
    44581 ttacaccagc taaaacagcg gcaatcgtga atgtagatga tttgaatacg cgaagttcca
    44641 agaatggatg ttccaaatgt aattgacgcc atgaaaacaa tgcgatgaaa atcacaccaa
    44701 caactaagaa accaattact ttcaagcttc cccagccgtc atctcctaca ctagaaaatc
    44761 cgtaaagcaa actaccgaat ccaatcgttg ataaaatagc ggataaaata tcaatgctag
    44821 gatttgacaa tggaataacg ctcttcatta ggaagaatgc taaaaccaag acaagaacaa
    44881 cgattggcag taccatacca aacaagtcac gccaatggta gttatcaatg atataaccag
    44941 ataatgtagg gcctaaagca ggagcaagtc caataacgat ccctgacatc cccattgctg
    45001 atcctctttt ttctggcggg aaaatagata gcatgatatt ttgcaataat ggcatcgata
    45061 ctcccacacc gcaagcttgg atcaagcgac cgattagtaa tgtactaaaa ttaggtgctg
    45121 taaagcaagt aatcgttccg atcaaaaaga tcgtcatggc agaaagatat aaatttcttg
    45181 aactgaattt attgatcagc catgctgtta ttgggatcat aatcccgttg actagcaaga
    45241 aaccagtcgt taaccattgc acatcagacg ctgaaatatc gaaagctttc atcaatgtag
    45301 gaaaagctgt cgtcaataag gtctgattca aaacggtaca aaatgtacca attaataaga
    45361 caacaactaa aagtgtccga ttatatggtt ttccgtaaat atctacgggt tggttgttac
    45421 tcatatttct ccttctttct gaattttttc tgaaactact agaatacttt agtcctaaat
    45481 tcgctatttg tcaacctact tgacacataa gttaatacgg atataattaa gggaagcaag
    45541 aggacaaaag gagtgacaaa aatggttggg gcttccctta aacgtttgtt ggaaaatgat
    45601 caattggttg ttggcatcag tgaattaagt gatatcgtag gtgtgtctcc acgtcaatta
    45661 aggtattggg aacaaaaggg attcattcgt tcgatcgaag ctgatgcgaa ttgtcctaga
    45721 aaatatcgtt tacctacagt cataaaagta gaactgatca aaaaatattt agatgacgga
    45781 tttactttgt caaaagctgc agaaaaggca gaaatccgcc aaaagaaaat gcaccatgtc
    45841 cgtaaagttt tttcaaaagc aatcaaagat atcgaattga tcgacgaacg ctatacgatt
    45901 ttacaaatcg gtaacttcaa agaagagaat gctaagatgt acatcattca tgacaacgag
    45961 acagacgaat taacttacaa gctccttcct gtcgatgaaa aagctgacta cgaacagctg
    46021 ttaaaagaac tctaaacgcc actaatctcc taagtaccaa gctttttaat aagtagatgc
    46081 tacataaata cttgaaaaaa ggcctgtttg aacataagaa tatccgaacg gtgatctgcg
    46141 tttctttcat atagaaagga attctccatc actgttcgga tattttttta tttttttcgg
    46201 ttactttctt ctaatacatc tgttagccaa tttgcatatt ctgtttcccg attgatcgca
    46261 tgcgctaaaa tcaggtagtg tccgtaattt tcatgttttt ctttttcagt aggaaagatc
    46321 gttttttttc gctctaatag atgaagtcgt ttttcttcat gaaggtcacg ctgctgctga
    46381 ataatttcta ctaatgccgg atcattcacg tctttgacga aatacaattt cagaacaaat
    46441 tcgtctcgat taaccggtag ttcattggtg ggtgtatgaa tccactctgc caattgttcc
    46501 ttcccttctt ctgttaattc ataaactttc ttttccaatt tttcacctgt gatctctact
    46561 tgatgctgaa tgattccttg ctcttccatt tttgcaagtt caggataaat ctgactatgt
    46621 tttgcttgcc aaaattctcc tatttcattt ttgaacgatt gattcaattc atatcctgtc
    46681 atcttcttct tacttaataa ccctaacaaa atatatcgaa gtgtattttt tcttgccatc
    46741 agttttccct ccttgacaaa tctttaaatc cattctatca taaatgagta atcgaaaaca
    46801 tgtagtttat tacatgtttt aatttataag gagatgctta aacatgaaag aatttaaaga
    46861 attggaagat tttattggaa ctcactttat ctatacgtat gataatggtt gggaatatga
    46921 atggtatgcc aaaaatgacc atactgttga ttaccgtatc catggcggaa tggtcaaagg
    46981 ccgttgggta aaagaccaag aagctgatat cgttaaattg acggacggcg tattcaaaat
    47041 cacttggaca gagccaacag ggacagacgt ggcattggac tttatgccaa atgaaaagaa
    47101 acttcacgga atgatctttt tccctaaatg ggttgaagaa catccagaaa tcactgttac
    47161 tttccaaaac gaacatatcg cagaaatgga agcagctcgt gaaaaatatg aaacttatcc
    47221 aaaattactt gttcctgaat ttgctactat cacttatatg ggtgatgcag gtatagacaa
    47281 tgaagatgtt attagcgaag aaccatacga tggaatgcca gatgatatcc gtagcggaaa
    47341 atatttcgat gaaaattata aacgagtaaa ataaggttgt gaaagagagg ttgtgaaatc
    47401 agcgttctga cctgagtatt agagaaagaa acggaaaaat agttcctcat gttttgccgt
    47461 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttaagaaaga ggttgtgaca
    47521 gaagtggtca gcttcaagaa ataagaagga atttacggaa attgcttctc aaatttttgt
    47581 aaaatttcgg cttatttccg aagaagctac ttttggaaca ccgtgaatca gaggttgtga
    47641 aaaagctgtt ctgcatcaag taataagaac agccaaataa aaatagctcc tcatattttt
    47701 tgtttggttg ggcttattac cgtagatgca agcttttgaa caccgtttat caaagattgt
    47761 aacaactgca tattcacaca gttaaattag tgacttcaaa atgaaataat gctcccagca
    47821 aaaaattagg ctgggagcat tatttcgtta ttctgttcat caaaaatcag cgttttgatt
    47881 ccatttcatc acaagttcct cctcatttac tgcttcatct ttttgctgtg tttgaattgt
    47941 aaaaccttgt gaaagataga aatgaatagc tgattgattt ttttgataga ctgtcaaagt
    48001 cagtgtttta ttggtttctt ttattttttt cattagttgc gtaccgattc tttgttgccg
    48061 gtattccctt ttcacaaaga ttcctgcgat aaactgatca atcaccccag caaaaccaat
    48121 aatttcttga tctatttctg ctaagtaaat cactgcatct ggtaaagctt tcctaacaga
    48181 ttctacttga ttctcccaat aagttgatgg aatgaattca tgagaatcca agttagcatg
    48241 tagccaaatc gatagaacag attccagttc tttggcagac aattttgttg ttgtgcggat
    48301 cattttttct ctccttactt attcattgat tttaaaataa aaaccatttt tttgaagctc
    48361 ttttacaagt tttcgcccct caaaaaagta tatcttctct tgttttactt ttctaatacc
    48421 aaaagaaaca gcaactttag gatcatcagc tgaaacgatt cgataagtcc caccaggcaa
    48481 ttgatccagc ttgactttcc catgatggtc gctttgtact gaaaacggca caccatttct
    48541 agtaagtgtt tttttcttat gtttcaaaat gaatgaacta ccatttaccg gtttccctga
    48601 cgaatcacta ataataaatg aaaaatcaag cactttttta cttaatctat actgatagat
    48661 tagtcgtccg ataaacacta acagtatgac tacagctatt atcgttcctg caagaataaa
    48721 ccattgccgc atggttcttt cctgatttcc ttttccttct tcttttttta ccgattcgtt
    48781 ataaggaatc cgatgtcctg tcactagcaa acgatgcgtg ttgatcatat acggcgtaca
    48841 tgtaagcaag gtaactaagt cttctccttc tgcaatcgtt agaacagacg tgtcatctgg
    48901 cagtaccact tgtatatttt taacttgata agccaatttc tctcccaaaa cagtcaaaat
    48961 aaacgtgtct ccgttttcta aacgatctaa ttcggtaaat aaccgtcgat taggtaaacc
    49021 gctgtgagca gagatcactg aatgcgtcga atttccacca agcgggaaag aggttccttg
    49081 caagacgcct gctccatttt caaggatcct accattgagc cgatcataaa gtgaaatatt
    49141 taattggatc ttaggtatat tgatacttcc gatcagccat tcctctaatt ggctggatgt
    49201 tgtactttca tttctgttct tttcattgaa aggatcagta ccaggattga atccttggtc
    49261 agttaatcgt tggtttttct cctccatttt ctttcgctgc acttcaattt cagctgcggt
    49321 ccgtttttgt acttgttcga tacgtttttg atcaataaaa ctatttaaag aatccacata
    49381 aaatggatat aaagcgatca gtaaccctac gacgatacat attaataaaa acaaacgaaa
    49441 caaaagtcga cgagactgtt tttgcttact ctgcttcaca tgtgtcctcc cttgcaagtt
    49501 tttttgttct ttcttttatt atatgggttc aggaaaaaaa tagaaaatga aagccgatga
    49561 gattgtgaaa aagctgtcct gcatcaagta ataagtacag cctcaaaaaa tagctactca
    49621 tattttttgt tgggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
    49681 agaggttgtg aaaactgcgt tatgctttga gtaataagag tgctagtcag aaaatagctt
    49741 ctcatatttt ctaggctagt gagcttatta ccgagaagca gctgattgaa caccatttaa
    49801 ggaagaggtt gtgaaaactg tgttatgttt tgagtaataa gagcgctagt cagaaaatag
    49861 ctttttatat tttctaggct agtgagctta ttaccgagaa gcagctgatt gaacaccgtt
    49921 taaggaagag gttgtgaaaa ctgtgttatg ttttgagtaa taagagcgct agtcagaaaa
    49981 tagcttttta tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc
    50041 gtttaaggaa gaggttgtga aaactgcgtt atgttttgag taataagagc gctagtcaga
    50101 aaatagcttt tcatattttc taggctagtg agcttattac cgagaagcag ctgattgaac
    50161 accgtttacc gagaggtcat gaaaaagcta ctttgtttcg agtattaaga aaaattctgt
    50221 ttcaaaacaa ggagatgcaa caaaacttat gtcgcatcct ctctccttag aactttcact
    50281 tatgtttttc ttgtaaaatt cataaatatt ttcatgaatt ttctaatttc ttgcctacct
    50341 ctcgcatagg atatatgatg aatttatcta aagtttgcaa aggagttcga caattgaaaa
    50401 aatatggccc atttttagca atttttttca ttgctttggc atttctcatg cctcatttat
    50461 tcacacataa actaattttg ggtgctgatg ccccattcca ttacaatcgg ttctatgatg
    50521 ccgcgcaaca aattaaatat ggggatttcc attattttcc ttccctttat ggattccagc
    50581 aaagtggcag gatcgtgaat gccgtttacg gcccattttt cgcttattta caaggattga
    50641 tcgtgttgct tgcaggtagc tggtttcatt atcagattct ttcaaatttc attctttatc
    50701 ttatttcagg attttctatg ctgattttac tgagatatgt caatattcga aattgggtaa
    50761 gtgtaccagt agccgtttta ttcatgagta cgtattctat tcaatactgg tctatcaacc
    50821 aaggattcac tagctggggg acttgttttt atcctttatg tatgatccct attgctgatt
    50881 tcgtccttca taaaaagttt cctgttatca aagtcgctgt ctcagtggga cttatgacac
    50941 agatccattt actgtcttcg atcatgttag ctttgatgta tattcctttt tatctttatt
    51001 acttttttaa ccagactgaa aaaggaaaag cactgcttca actgattaag tcggtcttgc
    51061 tttattttgt tctgacagca aatatttggg caggattagc tattgtttac agtgggaata
    51121 agattcttcc tccctttgtt aacagagaaa tgagtcaaaa ggcgatagat ctggatggca
    51181 gttattggct ccattaccct aaggcctttc ctatattgat attatcaggt tttttgcttt
    51241 ttattttctt tcaacaaaag aacaccttat tccaaaaaat cgtgttgtac acaagtcttg
    51301 ttttcttttt tctttctact agcttgattc catggacgac attgatcgaa aaaaatgtgc
    51361 cttttattag tttgatccag tttccatttc gatttttcgt gccatttacg ctattgcttc
    51421 tactttacct tgccctttca ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa
    51481 gtatatttgg cattcttatt ttttcagcac agacgatcca gaacttatac cagcatctgg
    51541 aaaagtggga aaatgaaaca tttgtcagtc gacatactta tctattcgat acgccggaag
    51601 aagcaaggaa aacattcttc aacaaagata tgtctaccag cttgttcgtt ttccaaaaaa
    51661 ccacacctga ctatctgcca atctataagg agacaaaaga caataaatac gaccggtata
    51721 acgaagaaat actagaaaac gaaacaactt ttataaaaac acatccaggc ggtcttctga
    51781 caatcaagtg gaaaaacact ggggacaaaa ctgttcatat ccctgttatc gtctacgatc
    51841 ggactgtgct acagcaaaat ggaaaaacat tgactgatta tgaagtaaca gatatcggga
    51901 ccccaatcgt gaagcagcaa aaaggaatca acgagctcac gcttcattat caaacgccaa
    51961 tctatttcta tttcattctt tctcttactt tgattggctg gtttaccttg ctatgtttat
    52021 ttatttatca tagatacaag catttacgtg cttaggcaaa agggataaat gcatccgatc
    52081 aaaaaactct ccaaacggta taatcgtttg gagagtttta ttttaatcag tttttttatg
    52141 ttatatgtta tattttttat tcgttcacat ctaattcgat ttccaccccg tagtgatcac
    52201 tgatgattgg actatcgatc ccatcaaaaa tccgttgata ttttttaacc tgcattcctt
    52261 cagggacgaa aataaaatct atacgcaatt tttctgtatt ttgttcccaa ccatcgattt
    52321 tcttttccac agtagctgct tttccttctt cattcgcgac aacatacgaa tcttgaagaa
    52381 gaaggcaact ttctcttaca gtataataac cttgagagtc tactggatta ttgaaatcac
    52441 ctaggaaaaa aagtggtacg cgagtttcca aaaagtagtt ttccaagttt ttccactcaa
    52501 aataaaagcc acttttttta tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt
    52561 cctgatcatc tatctttgtt ttcccaatca agatcttgcg tgtctgataa tcttctttct
    52621 tttgagattg ggagacggta aggacctgac tttccaatgt ttttttcgca agcagtgcat
    52681 ttccttcttc atatttatca taaccgatgt ggctcatttc ccaactccaa aaatactctt
    52741 ttccgtgttc ctttaaaagc tgaactaaac ggtaggcaaa attatcttta cggataggta
    52801 cttgattctt taccggacag aacttcatta gcttcgcagg ttctacttct tcagattcca
    52861 gtaattggtt gatttcttgt aaagcaatca cttcataatc ttctaacagg atcttatctg
    52921 ctaattcttg caattttttc tctggttctg gctcaagcca gctatgcgtg ttgagtgtca
    52981 tgaatttcat tggttttctc cttgtcttca tttcgaaaca aaagggttgt gacaaaattt
    53041 ttgtcacaac ctctccagca aatagtgttc tcctgctaac tcctcagtaa taagctcaat
    53101 ggaccaggag ctattttttg tttggctgtt tttatcactt ggtgcagggc agctttttca
    53161 cgacctctcc actaaacagt attcaaactg ctgcttctcg gtaataagct cactagccta
    53221 gaaaatatga aaagctattt tctgactagc gctcttatta ctcaatgcat aacgcagttt
    53281 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    53341 aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat gcaggacagc
    53401 tttttcacaa cctctcgata aatggtgttc aatcagctga ccttaggtat tagttcaaaa
    53461 aacggcaaaa tacgaggagc tgttttttcg ttttgtttct ctaatactca ggtcaggaca
    53521 atgatttcac aacctctcct tacattttaa tggagccgat cactttacca gctgtcactg
    53581 tacctaattg cttgatcact acttcttcta ctttatcgtt tgtaaatacg atcatgatcg
    53641 ttggatcttt ttcttcattt tggatcacct caagatccat ctcagctaat ttcgttcctg
    53701 ttcctacttc ttggccttct ttgacagcaa tctcaaaagc tggctgattc atttggactg
    53761 tatcgatacc catatggatc aaaacttcga taccatcttt cgtctccagc ccaattgcat
    53821 gtttgcttgg gaaaatactt gtcacttttc cagcgatcgg cgcaacaact tctcttgatg
    53881 ttggaataac agcaaagccg tcacccatca ttttttgtga gaataccgga tcttttactt
    53941 cagataaagg aatcacttct ccagaagctg cggaaactag ttcttgttct atacctaagt
    54001 attgcttact atctgcttgt ctatttcctg tagaaaccat ttctggctct ggaatctctg
    54061 ctcctgattg cagcagatct tcgatatctg atttcaagac atctgctttt ggtccataaa
    54121 ctgcttggac tccattgtct ttgacgatca atcccatcgc ccctgctcgt ttccaggctt
    54181 cttcagtccc tactttttct cgatcattta cacttacgcg taaccgagtc atacaagcat
    54241 cgacatcttt gatattttct tttcctccca gcaaatgaat gatctggacc acttgctgat
    54301 ctgctgttcc tgttccgctg ttagatacaa cggtttcttc actgtcattg tcgtaatttc
    54361 cgttacgtcc cggtgtagca aagttgaatt ttttgatcaa gaaatttgct aagaaatacg
    54421 taacaacacc aaaagcgatc acacacaaca cgaagttgat aagatctcca cctaaacccg
    54481 ctttgatagc taacggtgtc cgagtcagca attcgatatt cccaaatgaa tggacccgca
    54541 atggcaagat atctgccatc gcaaatgcag ccccttggat cactgaataa atgacatata
    54601 atggcacagc agcaaacata aacatgaact ccaatggttc tgttacacct gttagaaaga
    54661 cagccaacgc tgctgaaaaa tacattgatt tatatcttgc tttcttatca ggatcgacat
    54721 tacgatacat cgcaaaagcc attcccatca agatacctga agaaccaatc atttgtccta
    54781 ctttgaaacg agccggcgtc cagttatgca acacaaactc atattgcgac atatcccctg
    54841 cacctttaag attcactaaa tcagtggccc aagccaacca taacggatct tgcccaaata
    54901 cttgtgtccc cgcctgtgcg cctgataaaa tctcatacga tccgccaagc tgtgtatagt
    54961 tgataggaat cgtcagcata tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc
    55021 caaataaaaa cggtgctaga atcggtgcgc tttcttgtga ttctgcgatc cataaaccaa
    55081 aattattgat tccagcctgt acgtttggcc acaccacagc aagaatcaat gccacgattg
    55141 ttgaccataa gataacaaca aacggaacaa agcgctttcc attgaaaaat gacaaagcat
    55201 ccggcaattt tctataatta tagtacttat tataagccat tgccccgaca aatccagcga
    55261 tgatccctac gaatactccc atattcaaag ctggtgcttc caatacactt gtaaaaaatc
    55321 ctttaaccat gatcttcgta ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca
    55381 tgtcacttgt gacaccaaag atagccccag tgatccgatt gatcaaaata aatgagatcc
    55441 cagctgcaaa tgctccgcct gctctttctt ttgcccaact tcctcctatt gctaaagcaa
    55501 ataaaatatg aagattacca ataattgccc aaccaatatt ttcaattact ccgctcgttg
    55561 tgacaagcgg cgtccaatca ggattgatca agggaattga tttccctata ctgatcatca
    55621 acccagctgc cggcataact gctaccacaa ccattaaagc ctttccaaat ttttgccaaa
    55681 actcaaaact aaatatcttt ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa
    55741 acgtattata gcgtgcgttt tcttttttgt aaatgcttta ttttttattt ttctgtctat
    55801 accattctag aaaacctata atgcgtgtgt tcccttctat aattttctcc taaaaattta
    55861 tttttttatg cttttttcta tgaaatattt tcttttgtct attgacttgt gcaatcggtt
    55921 gcgtttatta tgaacgtata aaacagctca ttaggaggag aaaaatgaaa cagatcaaac
    55981 gactatttgc tattgatcca tggaaaatca gcacgacaca attagataaa aaaaatcttc
    56041 gactgcaaga atctttaaca agtattggaa acggttatat ggggatgcgc gggaactttg
    56101 aagaaactta ctcaggggac caccatcaag gtacttattt ggcgggtgtc tggtatcccg
    56161 ataagacacg tgtcggctgg tggaaaaatg gttatccaga atactttgga aaagtgatca
    56221 acgccttgaa ctttatcgcg atagatattt cgatcaacga tcagctgatc gacttggctt
    56281 ctttggaacc agaagacttt tactgggaat tagatatgaa aaatggtgta ttatctcgta
    56341 cattcacaat cactacttct actaataaag tacgcatttc cttcgaacgt tttctgagca
    56401 ttgtcaaaaa agaatcagct tatatctatt taactataga aatgcttgaa ggtagcggaa
    56461 cagtcaaact cgtatcaaaa ttggatggaa atgttcaaaa cgaagacagc aactatgatg
    56521 aacatttttg gaatgagatt gatcgtggaa ctatagaaga aatcggctat ttgacgacta
    56581 aaactattcc aaacaacttt gggattgaac aatttactgt tactgcagca atgcgccatt
    56641 tcgttaatgg aaaatcagtc atccctgact atcacgaaga tactttagca ttacatggtt
    56701 gcttgtctgc agaattatct caaggtgaga catggtcagt ggaaaaagaa attatcgttc
    56761 ttactagtag agatattcca gaagcgcagc aagcagaaaa agctgtctca gaattaacga
    56821 accagacaaa caattatatc caagcaaaag aagagcaaac agcggcttgg gcagaacgat
    56881 ggaagcttgc cgatgtggtc atcgaaggag acgaagaagc acaacaaggt attcgattca
    56941 atttgttcca actattctct acttactacg gcgaagatga acgattgaat atcggcccca
    57001 aaggattcac aggagaaaaa tacggcggag ccacttactg ggatacagaa gcgtatgctg
    57061 tccctcttta tcttgcttta gccaaacctg aagtaactaa gaatctgctg aaatatcggc
    57121 ataatcaact gccgcaagca atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt
    57181 atcctatggt caccttcact ggtgtcgaat gccataacga gtgggaaatc acattcgagg
    57241 agattcaccg aaatggcgcg attgcctatg caatctacaa ttatacgaat tacaccggcg
    57301 atacggaata tttgaaaaat gaaggactgg aagttttagc agaaatagca cgcttctggg
    57361 cagaccgtgt ccatttttca aaacgagcaa ataaatacat gatccacggt gtgaccggcc
    57421 caaacgaata tgagaacaat atcaataaca attggtatac aaatacgatc gcagcttggg
    57481 tcttgcgtta cacgcgagaa aattatttaa cttatcaaaa agaagctact atcactatca
    57541 gcgaagaaga actagccaaa tggaaagata tcgcagagaa catgtacttc ccagttgacg
    57601 aagaattagg aatctttgtc cagcatgaca cattcttaga taaagatctc atgcctgtat
    57661 ccgatattcc cacaagcgat cttcctctaa atcagcattg gtcttgggac aaaatcctta
    57721 gatcttgctt catcaagcag gcagacgtac tacaggggat ttatttcttc aatgatactt
    57781 ttacaaaaga agaaaaacaa aaaaactttg atttctatga accaatgact gtccatgagt
    57841 cttccctttc gccaagtatc cattcgattt tagcagcaga attaggaatg gaaagcaaag
    57901 ctgtagaaat gtaccagcga acagctcgct tggatttgga taattacaat aacgacacag
    57961 aagacggctt gcacatcact tccatgactg gtagctggtt gtcgattgtc caaggatttg
    58021 ctcaaatgaa aactgcccat gatcagttga gattcgcacc atttctgcca aaaacttgga
    58081 aagcttattc tttccatatc aattatcgcg gacgtttatt gttcatctca gtcacagatg
    58141 aacaagttac tttcgagtta ttagaaggtt ctcctctaga aatttacgtc tatgaacagt
    58201 cttattcttt agcggatcgt ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa
    58261 ggggtattgt ttgatttaga cggtgtcatt accgatactg ctgaattcca ttatcgtgct
    58321 tggcgaaagc ttggtcagga aatcggcatt acgatcgatc gtacattcaa cgaacagcta
    58381 aaaggcgtca gccgagaaga ctcactctct ttgcttttag ctcatggcgg aaaagaacac
    58441 tctttttcaa aggaagaatt tgcagaatta gcgaaaagaa aaaacgatta ttatttagag
    58501 atgatccaga cgatcgaacc aaaagatgtc ttccccggag tcgtgccttt actagattct
    58561 ttaaaagaag aaaaaatcaa gattgctctt gcttctgcaa gtaaaaacgg gcctttccta
    58621 cttgaaaaaa tgggtcttac accttacttt gatgctattg ccgacccggc agaagcaaca
    58681 aacggaaaac ctgcgccaga tatcttcctc cttgcagcaa aagctgtcgg actaaaagca
    58741 gaagaatgta tcgggatcga agacgcgcaa gccggtatcc aagccatcct ctcaagtggc
    58801 gctcaaccaa ttggcgtggg acgtgctgaa gaccttggag aggagatccc gcttgtccct
    58861 gatacgactt ttcttacgat cgattacttg aaagaaaagt ggcatgacca tggataatcc
    58921 agaaatctcc gtcgtaccag ttccagagac tgaaataaag aaaatcacat tgaagaacga
    58981 ctacttagaa gtcgttcttc tcaattacgg tgcccgcctg caccaaatct tcgcaccaga
    59041 caaagaagga aaatcagaaa atgttttatt gtcttacgat cgtttcgaag atgttctaac
    59101 agacaaaagc ttttttggag caactgtcgg acctgtagcc ggaaggatcc gggatgcttc
    59161 ttggggtgag catagattgg agaaaaactg cggatcacac catatccacg gtggcacgaa
    59221 aggctggtct ttccaatttt gggaagtaga agttttcaaa actccatcat caataggtgt
    59281 ggtcttttac ttaaaagatg aattttctac ctatccagga ccaatcactg ctacgatcac
    59341 ttaccgttta acaaaaaacg aattagagat gacaacctct gctagtagcc ttgttgaaac
    59401 catctgcaat ccgaccaacc atgcctactt caacctttca ggaaatggaa caagagacat
    59461 ctatgaacat cagttaacgg tctttttaga tggtgtgctt gaattagatc aagaaaaact
    59521 gcctactgga aattggacaa aaaaagaaga actgccaatc gactttagaa aatccccaac
    59581 actgcaagag atccttgcat gttatccaga tggactagat gatgtatttc ttttgcatca
    59641 tcctaggctt tccaaaactt ccttacaatt attcgagaag cattctggtc gcaaaatgac
    59701 tatagcaact tctaataaaa gtatggtatt attttctacg actggattcg aagctgattt
    59761 ctctgtcaat ggaaaacaga tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat
    59821 ccctgatatc gttcatttcc ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg
    59881 gatcagtcag acgatttatc ggttctctgc acaataacaa gagaaaggtg tttttatcat
    59941 ggctattacc gtaaaagatg tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg
    60001 tgtcatcaac gatcatccta gtatttccga gacaacaaag aagaaagtcc gaaaaattat
    60061 ggatgaacta ggttatgttc ctaacatgac tgcccgcaat ctaggaaaac gcatttccag
    60121 tgccatcggc gtaatcttac cccctcttga ttcaaaagag cggatcggta acccatttta
    60181 tcttgagatc atggaagcaa tcaacgaaga agcgcggaat tatgatatga ctacagcaat
    60241 cgctactgct aaaaattttg aattgctgtt agagaacgtc cagcgtatgc atttacaaaa
    60301 acaagtagat ggttttattc tagtgtattc agatagtcaa gatcctgtca ttgattattt
    60361 ataccaaaac cacatccctt tcactttgat cggacagcct tatcatcatg agaacgaaat
    60421 cgtttatgta gataatgata accagcttct aggtaaacaa gcaacagaat ttttgatcca
    60481 gaatggacat aagaatatcc tgtttgtgac aaacacgact catgaaagtt tgtatttcga
    60541 acgatatttc ggttatcaaa aagcaatgat gatggctggt cttcctgcgt tatccagtgt
    60601 agtactagag catccggaag attatctgtc ctttgagaaa acgttgaaag aaaacgaagc
    60661 cacagcattg gtggtcattg acgatatctt cgcccttcgc acgatgcagt tggcgcaaat
    60721 gtacggctat gtcattccag agacactctc cgtcattagc ttcaacaact cgatcttctc
    60781 aacattaaca cacccttacc ttacaagtat cgatatcgat atctccgaat taggacgtgt
    60841 cgctatgcaa aagctgcgcg aattgatcca tgaagaattg agcagtggcg tacgtttggt
    60901 catccctcat cgcttgatca aaagagagac agtcgttcct ttagaaaacc catactaaat
    60961 agctgactca ctaaatacgc tgaccattta tcctatggtc agctttttat gtaaaagaag
    61021 atactacttc taacacattg tttatctgta aaaaagagcg tgggacaaaa gtaaagaaca
    61081 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa
    61141 taagctgaaa ttcacaaaaa tttaaaaagc aattttcttg agtttcttct tatttcttgg
    61201 agttaaacgc ttctgtccca gcctcttttt tccttccttc aattttttac acgtcgacga
    61261 ttcaataaaa aaccgcctaa tattgtaaag acaggtgcaa gaatcgagaa aaaggcgaat
    61321 ggcaagtaat gcaacgcgct aactttcatc gttcccataa tgaatgtgcc gctgactccc
    61381 cacggtacga gagaattgac ggtcgcacct gaatccgcta gtgttcgtgt caaataggtt
    61441 tttgataagc caacgtcatc aaaagaacgc ttgaatgttt ctcctggaag aatgatggac
    61501 agatactgtt ctccaacgat cagattgatt ccgatacagc tcaaggcagt aaacccgact
    61561 aaacgagaag gtcggtccat cttttctttt aattcttgaa cgatggtatc aacgatcaga
    61621 tatttgatca gtaacccacc taatgccaat gcaagtaaaa tcaaactagc agaacctaac
    61681 atactcatga tcccgcctcg agaaagcagt tcatctagct ttggatcagg tgtatttgcg
    61741 acataaccat tcatcaagat gtctgctgct tttccgaccg tcaaactttt ctcatggata
    61801 aaagaaagaa cgagagcagc tgtcgatccc gctagtaatg tagggatagc cggaatcttt
    61861 ttgattgccc ccccaaataa aataactagt ggaatcaatg tccaaggaga gatccaaaag
    61921 ctcgctttca tgttttggat catttcattg atactagcta gatcggctga atgactgtta
    61981 tgtcctaata tcgtataaaa aatcaacgaa aggacgaatg ctgggaaatc cgtataacgc
    62041 ataccagcga tatgttcgaa tagattaact cctccaatac ccgctgccaa gttggtcgta
    62101 tctgataaag gcgagcagtt attccctaaa aaggcaccgg aaacgactgc gcctgtagtc
    62161 attgcatcat tgaatccaag taaatgaccg atccccaaaa aagcaattcc aatcgtcgaa
    62221 atcgttgtaa atgaactgcc gacagtcact ccgacaattg cacaaacaag aaatgctgtc
    62281 ggtaaaaaga aacgcacaga aacaagttta aatccataaa ccataatcgt agggatggtt
    62341 ccagataaaa tccatgaagc aactaagaca ccaatcaaaa gaaaaatcag gattggaatg
    62401 atccctggag tgattccttt gatgattccg tcatggatct catcccatgt gaacttttta
    62461 aatctgccat aaaacaacaa acacatgaaa gctgcaagga tcgggatatg tggcgagagc
    62521 tggaatccga tgatcagtac tcctaggagt gccaacaaca cgatcaatac agcgatactt
    62581 tcttttaaag aaatattttt attcatcgtc gatctcctat ttcaaatgaa aaggccctgc
    62641 catgaaaaaa ctattcactt ggcaggacct cctactaaat cagcgacaaa acgtcggctg
    62701 aaagacttat ttttttattt ttcttctaaa cttttttcta cctcaatccc ccaagaatct
    62761 ttcttgaagt gattgatgat caatggaata acgaacatga ccactgtgat agctgctact
    62821 aataatgcat cactcaatgt cgctgtacct aatgtagcaa atacgacaac gatgatagag
    62881 aagatcagca tgcagctgac tacccaagct aatccgtttc cgtttttacc aattcggtaa
    62941 ggacgttcga attctggatg tttcttacgc attttaagca atgaaacagc aatcaaaatg
    63001 tacacgatac aataaataac agttgtagca tttgtaagtg tcaagaatac agcacttacg
    63061 tcatccatta caccataaag caaagcaaat aatgaaataa cgattgattg tgttagaacg
    63121 acatttctag atacgccgta tttgttttct ttatggaatc cgaattttgg tggaaggaat
    63181 ccttcacgag ctacttggat gattgttttt gatggtccag taacccaagc tgataattgt
    63241 aaaagcacac caataaatac catgaagctg aagatatttc cgatcacttc tggtaagcct
    63301 aggatttgac agtcaatcaa gataggctgc gtgatatttg ctaattccat ttttccatct
    63361 ggtacgacat ttgctacaag catcgcattg atgacattca ataaaactaa tccgatcaaa
    63421 gcgataaata cgccttttgt ataatttttt gttgcgtctt tcaaacgagg catgtaaaca
    63481 gaactcattt cgatcccaac aaaaataaat gtgataccag ctaagtattt caatgaatca
    63541 atatgttcca agtcagggaa agcttttgac catgaaaagt cacctagata gccgtttgag
    63601 actagtccga ctttgaaggc tgccaaaaca cctaaaacga acatgatgac tactgggata
    63661 tataccccta gccatacacc gatgttcccg ccgacttttg ccatatcgaa tttcaagttc
    63721 aagatcgtga tgatccagta gataactaaa atacaaccta aaacgaacca gtgattattg
    63781 ccaagttcta cattaccaaa tgtgtttcct aacaatggtc ctaaagtaga agcgaccatt
    63841 accatacctg ggaacatttg cacccataat agccatgcaa cgacaaatcc ccatttgctg
    63901 cccagtgctg tttttaccca taactgagga ccgccttctt gaggaagcat cgttgaaagc
    63961 tcaccggaaa ccattgatat cggaccggca aaaaacaaaa ctgcaaaaat tgaataaaaa
    64021 atcaatgtcc aaccgactgc ggctaacgta ggaatactcc gaaccgttgc acacagtgcc
    64081 attgtcagac cgataaaggt cccaagaccc attttactta cttcagattt actcaccgtt
    64141 gacactctcc ttgatttttt ggataaaata acgatgtctt tgaagtagtt tcagcaataa
    64201 acgaaacaat tcagcgaata tttgagggga ttctttgagt ctattgcttg aagggtttct
    64261 tcaaagacaa cctcaaaaca aagggatcga agagcttagg tctccccttg ctcttcagat
    64321 ccaaacatgc gtcagaaaca gttagtattc aagctgtttt ctaacatgat taccttgctt
    64381 attttgcttc gcttgccaat aattggtctg cgtagatttt ttccaatttt tcttgaagag
    64441 ctgctttgat tttttcagca tattcttcaa agtgttcttc tttgttcatg tatggtgtca
    64501 ttacgctggc acgcaataca gttacttttc cggcacggtt ccattcttca tctgtgaatc
    64561 ctagttggtt cacaaattgt aatgggctgt tgccatagtc tgggatagca aagtcagtat
    64621 gtgaagttaa gaactcatta ccgtaaatgc ttcctttcac gtatgaagaa taatcatata
    64681 cgtcatggtt caatttattc attgcaacta agtcatcgtt gcctttttct ttgaatacgt
    64741 agtctaccat attgaaatct ggataagtta atggatgaac ttcgatttct ttgtctccta
    64801 ctttaaatga caagtcattc aagaagttgt agaaacgatg tgcaccttcg attgatgcac
    64861 ccattaattt accatatcct gtgacattca aaggtaagac atggtgtgct gcccaaacgc
    64921 tggctgctgt tgcccctgct tttgaacctt ccaagatata agccccaagt aatgctggga
    64981 tatctgcgcc tttttcaaat acatatgtcg caaagtaaga gataacgtct ctcatgcgga
    65041 tatctttgat gacgatacca ccggcagaat atggaacata gcccatttta tgtgggtcaa
    65101 tcgttacaga ttctgcttct tcgattgctt tatatgcact gtgtacttct tctaagatat
    65161 aatctttgtt ttctgtaaag acattgtatt tgtaatgaac atcttttaaa tcttcaaatg
    65221 gaatgaagtt gttgtcttcg tctaagaaaa tcgcacggcc gtatccgcca taagcagcat
    65281 ctacgtgtaa gtagaagtaa ataccgtctt tttctaatac gcgacgcaat gcaacgattt
    65341 tatcgatacc gtcgattgct ccttcttctg ttgatcctac aacaccgact acacctagga
    65401 ttggtgtttt ttcagctgct agaccacgaa cgattttttc aagttcgttg atgtccatgc
    65461 gatagttgtg gtcaactggc actggaatca cttggtctaa accgataccg atgatgtcgg
    65521 cagcttttag ccatgaatag tgtttagttt gtggaaccaa ccattttcct aatttttcta
    65581 aatgttttcc gctacgtgct gagtgagctt tgatttcgtc gattttttct gggacgctgt
    65641 ctaacagatt catgatttct tctgttgaca agttcattaa ttcccaatcg ctttttccag
    65701 caactagttc aggtgtcact tctttcattg caagcggcaa tgatttgatg ttgcgggcat
    65761 accaaagtcc ttctaagttt gctaaagaac catcagcaac gatgtggccc cagccatctt
    65821 tatagctcat taattttgca aattccattc ctacttcttc ttccatttgg cttgttgcag
    65881 gagaagattc ataggcaacg ttattaccat tccatagcat agcaaaattg tacgctagta
    65941 aagatggcat caatgtttca gagttcatat gaccccagta acgtccggca ttatgccaag
    66001 gaacggaatg ggtacgcatc cgcgctgaaa tttctgatag aacatccttt gttttattca
    66061 cagtgtgttc aaaactagca ctgctttttt cttctggcgt aataataggc atgtcttgag
    66121 gcatgtagtt ttgacgccag cctaaatgtt catccaccaa ttcattcaac aaagctttat
    66181 agatttggcc gttctcagct ttgtccccaa tgaatagggc atttaggttt agatctttcg
    66241 acaatgattc actcatagtg aaaccctcct aaaaaaatat ttaaattttg tcgagcccta
    66301 cattacacct gttcttctat tctgtaaact aagttttggc ctgattttta atcatatttt
    66361 aatatgtatc aattatccga attgttcaca cttttgaaat aattaaaaaa aacgagggaa
    66421 tttacaaatg taaactccct cgcaaagatt gtattaacga caaccagcgt tctattttag
    66481 gatgggtttc aaaattggaa tgttgtaata gtatttccct ttttcaaggt aaaaacttac
    66541 taaattctaa tcaattactt tggccaaaaa gtaatttttc ttaccttttc gaataaccac
    66601 aaacttacca tcgaattcat cggatggatt tatgacaaaa tttaaatcgg taacgcggtc
    66661 accgttaata cttatagctc cgtttgaaac atcttcacgt gcttgacgtt tagatggttc
    66721 aatttttgtg gaaacaagca gttccacgat attttcaggt gtgcttgaaa tttcaacgtt
    66781 cggcatttta ccgaatcctt gcgcgatttc ttccgcattt agttctttaa tatttccaga
    66841 gaacaatgct tgcgttattt tttgcgcttc tttcaagtct tcttcactat gcacaaatct
    66901 tgtaacttct tctgccaaac ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt
    66961 tttcgctaag tcttcgattt cttcttgaga caagaaagta aagaatttca agtatttcac
    67021 tacatcgcga tcatcttgat tcaaccagaa ttggtagaac tcaaatggtg aagttttctt
    67081 cggatcaagc caaacggcac cgcctgcagt ttttccgaat ttcgttccgt cagccttcag
    67141 catcaatggg atcgttaaac cgaacgcttc tacttctggt ccttctaatt ttcggattag
    67201 gtctaatcca gctgtgatat ttccccattg gtctgctcca ccgatttgca attgcacatc
    67261 gtatgtttta tgcagatgca aaaagtcgat agattgcaga atttggtaag taaactctgt
    67321 aaatgagatt cctacttcca aacggctggc aacgatatct tttgctaaca tcgtgttgat
    67381 gttgaagttt ttaccgtagt ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc
    67441 gtagttgttc acaaatgtga tatttgcatc tttaccaaac aattttctca tttgattgga
    67501 aagtgcgtcc acgttatgtt gcacttgttc catcgtttgc aacacacgtt ctgttttgcg
    67561 gccgcttggg tcgccgatcg ttcctgttcc gccgccaatc agaatatatg gatgatggcc
    67621 tgccaattgg aatcttttca tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc
    67681 accagttgga tcaacgccgc aataaagtga aatgctcttt tcttcaacga gctcgcgaag
    67741 tctttcttcg tttgtttgct gattaatggc gtcgcgccaa gttaattcat caataatatt
    67801 cattttactg acctccgtgt agttttttta tgaaagcagg aacctgctag catgtgtaat
    67861 taattatttc ttcaggatag gataggaaat aaaaaaagtc ccttggcata taagccaagg
    67921 gacgaaatct cgcggtacca cccaatttgt atgtctagat atccaacata cctctcgaac
    67981 atttaacgct gtcgcgtggc aatcactgcc aaagactcct gaaatgtaat tcgctttaaa
    68041 gcatgtactg gtttgcagcg accaccagct ttctgaaaca ttggctttaa caactacttc
    68101 gtttcgatct tcgtcttgtt attattaata tgtgtctatc atagagcgca actttttctt
    68161 tgtcaacaga aaaaggatat tttttttaaa ttcctcattt tttctttttt gaatccgctc
    68221 taattccgaa cattttattt tcaattattg ttaaaagaaa taactttttc tgtatttttt
    68281 ctgcaattta tcgagaagtc actttacttt gcctcttatt ctttcttcgt tacaataagg
    68341 aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa
    68401 attattttag gagccacttc tgctttaact atcgttattt taattctaag tttgtttttt
    68461 gtcatccgat catctacaaa aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa
    68521 gcaaaagaac cgacagatga taagcaacaa ctagttactc aagcaaaaaa tctggctcaa
    68581 agctacgatt atgaccaagc gattgcgtta ttggaaaaag atagtaagaa cggatctact
    68641 gaaacacaac agcttcttca aacactaaaa aaggagaaag aacaactgat cgactggtct
    68701 gatcctactc agatctctca tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc
    68761 ttccactctc aacaggcaca aggatacaaa gattatatgg tgactgttga agaattcaac
    68821 cgttcgatcg aacaacttta tcaaaatgat tttgttttag ttaatttaaa tgatttgatc
    68881 caaaaagacg aacaaggaaa tctttccttt aacgggcttg cgctacctga aggaaaaaaa
    68941 ccattggttc tttcacaaga tgatgtcagc tattatgaat atatggataa ttccggtttt
    69001 cctagccgat tggtcgtatc aaataacaat gaaatcaaaa atgtctataa agagaaagat
    69061 aaagaagaaa tcggtgactt tgacatggtt cctctgatcg atgcctttgt caagaaacat
    69121 cctgattttt cttatcgagg cgcaaaaggt accttggccc tgacaggtta caacggagtt
    69181 ttaggttatc gaacatcaaa atctcaatat ggagacaatg aaaaaacaaa aaaagagatt
    69241 aaagaagcta aaaaagtggc ggaacagttg aagaaagacg gctggagctt tgcttcccat
    69301 acatggggtc acttgaatat gactcaagct tcactagaag atgtaaaaaa agacaatgaa
    69361 ttgtggcagc aagaagttgc acctatccta ggaaaaacaa atattctgat ttatccattt
    69421 ggtgcagaca tcagcgactg gcagccttat tcagcagaaa atcaaaaatt tgcttacttg
    69481 aagagtcaag gtttcgatat tttctgtaat gtcgatgctt ctacaccagc ttggggacaa
    69541 ttaggtacgg atttctatcg aaatgctcgg atcaatatcg atggcattcg ttttcaatct
    69601 gatttagacg gaaaaaatcc tattcttgat ccattcatca atgtccatga tgtctatgac
    69661 aaagaagcac gcgaatctgc ttgattttac gtcatttatg aaccaaaaaa gccgaactga
    69721 cattttatcg tcagtttcgg cttttttatg ctttatttct tcttcggtgc gattcccttg
    69781 atcaggactt ctgtccattc tgctgtatat ggaagcagtt ttgcgtatat ctccggctta
    69841 gaaattcctt ctaccattgc ttgaaaatag aggatcaaga actcatccga atatttcaaa
    69901 tccacttttc cttctttccg ccctcgatgg aaaagatcaa gcatgatact gaaactttct
    69961 tgcgtatacg catccatcgc gtcagaaaat tctttttttt ctttttttgt atagtatgtc
    70021 atcaaatcga tataaaattg tttgttcact cgattgagat aacggatttt gttttcactc
    70081 atggcaatca gtgtttcttc aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc
    70141 cctaaatcat tcataaatgc aaaaaatact tcataaatca gcccttcctt gctgccaaaa
    70201 tatttaaaaa tcgtcgcttt acttacctga gcgttctcag ctaccttatc cattgtcact
    70261 ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt
    70321 ttctcttgtg ctctcttttc aaaaccgttc atttgtagag cctcccgtaa ttttaaactt
    70381 ttataatata tatagttcaa atttattctg aggtttatta taactatcta ttgagaaagt
    70441 atcaagctag caattccttg tttatgaatt cattgacaga aatttataaa aagaatatta
    70501 tgtactaaaa taataaaaaa agttttataa aaaggtataa aaccttttta ttaaactatt
    70561 taatcttgtt tagtatatat ttttagaaaa aggagtgggg tttttatggc agaaatcgtc
    70621 aaagtcacac atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga tgtctctttc
    70681 accgtacatt caggtgaagt cgttggattt atcggaccaa atggttctgg aaaatccacg
    70741 acgattcgga cacttttagg gattataaaa aaagacggcg gaacggcaac gatttttggg
    70801 caagatgtat gggaagatag tttatctatc catgaacgtc tttcttatgt tccaggcgat
    70861 atctctttgt ggggaaattt gactggagga gcgatcatcg atctcttcat gaagcttcat
    70921 ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct tgatcctaag
    70981 aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg
    71041 gctgttgatt cggatctcta tttatttgat gagcctactt ctggtttaga tcctttgatg
    71101 gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc catcctactt
    71161 tcttctcata ttctcagtga agtcgagcga ctagcggata aagtcgtgat catacgcgaa
    71221 ggaacagtcg tagaagaagg aacactcgat gaattgcgtc atttgactcg ttcgaccatc
    71281 actatagaaa cagaagcaga gaatagtcac ttagcacaat tgaacggtgt ccatgacttt
    71341 gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg aagctttcga tgtgatttta
    71401 gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat
    71461 ttattcatga gacattatga aggatgagga ggaagcgaaa tgactgataa attttcacgc
    71521 tggtccaccc ttttttctca aaaccttagc cgtgactgga aaaagatcct gatatggcta
    71581 gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt gacttccgga
    71641 caaggattag ctggattata cgaaaccttg caaaacccgg caatgattgc aatggtcgga
    71701 ccaactccag cttcgaatgc tgctgagtat actttaggag cgatgtatgc aaatgaaatg
    71761 ttgctttttt gcagtttgtt tgctgctgtg atctctgttt tacacgtggt gagccaaacg
    71821 agaaaaaaag aagaacttgg tctgattgaa cttattcgtt cctttcgagt cggacgtcaa
    71881 gcaaactctt tagctgtcat cgctgaaagt attctgatca atttgctttt agcaatatcg
    71941 atcagctgta cgatgatttt tttccaagca gattcgatca ctgcagaagg tgcttggctt
    72001 tttgggctat ctgtaggtag tatggggatt ttggggagtg ccgttgcact gtttgctgca
    72061 caactgatgc caagttcagc cagttcgaat ggtctgtctt tagcgttttt aggtggactt
    72121 taccttctac gagcaataac agatgtagct gattcctccc tttcgatgtg gaatccttta
    72181 ggctggtctt atttgaccta tccatttaca actaacaact ggattccttt gctttatttg
    72241 tttttgttca gtctaatctt gataatcagt tcatttgttt tagaggaaca tcgagatttg
    72301 aacaccggct atatccccga acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc
    72361 ttgcgtggtt tattattcta tctaaataaa gggatgacaa tcagttggct tatcactttt
    72421 ggtttgatgg gggcggcata cggctctatc tacgggaata tgcaacagtt tttagaaagt
    72481 aacgacttaa tgaaacagat gtttatccaa tctggcgtct caattgaaaa aaactttaca
    72541 agcacgatca tggtcgtctt gatttgtcta gctgctattt taccaattgc tagcatcaat
    72601 cgtttattta cagaagaaac ccacggacgt atgaatcagc tgttcgcaac aaaaatttct
    72661 aggaaccaac tttattggac caatatgtta ctagctattc tatttgctat attggcaatc
    72721 ctgattagtt ctggtgcact agggctttct gctttggctt cgatgaagga tgcgaaagac
    72781 atggatctat tgatgtttct taaagccggg ttcaatttcc tgcctgccgt tttagtgttt
    72841 atcggaatta gtggctttct attcggatgg cttcctcgaa tgggtaaact tgcttatatc
    72901 tacttagctt actcctttgc actcaattac tttggcggaa ttttggattt accagaatgg
    72961 ttttccaaaa cagctgtccc tagctgggtt ccacggatgc caaacgattc ttttgatctt
    73021 tccgtgttcc ttacgttatc atgtatcagc cttgcactgt tcgtccttgg ttctattggt
    73081 tataggcgaa gagacttact agaaggataa taaaaaagca tgtacaattt catgctagcc
    73141 tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt ttttatttac aagtcttcta
    73201 actgctcttt atcaatgatc cagacttctc gattctttct ttttacataa ccattctcct
    73261 ctaataattt aaattttcta gataaagttt cgggggtcgt accgataaat gcagcaagtt
    73321 ctttcatttt cattggcagg aaaaagtgat ccttccctgt cgctttagct aagtcaagca
    73381 gatagacagc gactcgttct tctacttttt ccatcatcaa aaaagttgtc tgctgctgta
    73441 cttttgttaa tttctcaacg ttgattgcta aaagtttcag actaagggcg ggataatgca
    73501 gtaagagttc cgagaaatct ttttgtctca atatgcaaat ttccgttttt tctagtgctt
    73561 cgccaaataa agactcattt ggaacgccta gcaactggct ctctccctca tatccgcccg
    73621 gctctattac tcgtaaaagc tgttcctttc cgctagcaga taattgatac actttcatac
    73681 tgccggaggc cacgatcact aattgaggtt ctccattcgg cgcaagaatc tgttctcctt
    73741 tttccacata gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc tgtactaagt
    73801 gattgaacaa aggaacaaat gaaacacata aatgatcatg actcataatc tctcctcctt
    73861 ctaaacagct atttctaata aagaaacata acttccctta ttccaactga taattttctt
    73921 ttatcatagc ataaacgtca agcgaaccag ataccagcaa atagagtgga tttctttttg
    73981 tcccttgctt tattttcctt cttttgagac cttaagtaat tgattcctat caatttttta
    74041 aatatttact ttttgaataa tcaaaaacaa accttcactc gcaagccgag agtaaggttt
    74101 gtttttcgca ttgcttattt cgtgttgtct attgaaattt ttccgcacct agcttttgat
    74161 atagtggtgc tagttcttgt aaggtttcag cggttatttg ctcaacgtgt tcgatttctt
    74221 gcggatagaa gaatagttta cccatttgaa aggttcggtc tctcatcgct tttttcaact
    74281 cttcttgttt ctttgtatcg gacaaatagt cagacaaagc taagtggtcg tcaagttcat
    74341 gttctggttg cggccatatg taataatcct tgatatcgac tgttttgctc cactctttca
    74401 gtatcgcaag caactggtta tacgctgcaa tgctaccata tcgttcttcg cttttataat
    74461 gttggtacat caaatagatt tggtattgtt tttgattcaa caaaactgct agacaggcat
    74521 tctcgtcttg acgagattcg cttcggtacg cgctccaaaa atgactgcgt aaattccagc
    74581 cattcgtcca gctttcgatt ttaggtttgc tcatgccgta catatcaggc aaaagtgcag
    74641 ctgtctggag ctgaatctct ttccattttt gccagtgttc tttatattct ttcttgattt
    74701 gttcaatctt atctgttgga tggctctctt tgagctgttt gaatgcgtag attggtcgat
    74761 caaacaactg ataatctgat tttccaagtt ccattgtttc gcctctttcc gttatctttt
    74821 gtattctacc tgaattttgc gatatggaca aatagaggtt gtgaaaaagc tgttctgcat
    74881 caagtcataa gaacagccaa acaaaaaact gcgatgagca tcaccgcagt tttattgaac
    74941 ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag aagcagctcc
    75001 tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt ttttagctac
    75061 caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat tccctgcctt
    75121 caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag cttctgcctt
    75181 tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt tttcttgaga
    75241 atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag atccgtataa
    75301 cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt tgaatgtatc
    75361 gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa cccctcttcc
    75421 catgttattt ccatggtctc catcgccaat cggggtatct agatcactga gatattgttt
    75481 gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt gaacggttag
    75541 tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct gtacattgct
    75601 ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag aaagaccttg
    75661 catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga cgcctctttc
    75721 ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc
    75781 cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt
    75841 agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac gatagcctgg
    75901 ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga atcctggttt
    75961 ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac
    76021 gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc
    76081 cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag tatacgtact
    76141 attttcaacg gcgatatcat catctacgat cacactttct acttcaatat cgtccatagc
    76201 agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct taacgatcag
    76261 caaaactcct tttccactat ccgcttcctt aatagccgtc tgaatctgat ctggagttgg
    76321 agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta caaacccggc
    76381 atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg
    76441 attcttggct acaacacggg aagattctac acgatgaaca agttctggat agctttttac
    76501 taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt ttttcaattt
    76561 attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga gtatcatctg
    76621 ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca tccaccagat
    76681 gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag ttcaataccg
    76741 cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca ttgactgctt
    76801 ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga gaaaaaattt
    76861 gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct gttacttttt
    76921 gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct tttacatagt
    76981 tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata aatttctaat
    77041 tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa aagagaggag
    77101 tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag tagcttataa
    77161 agggatcgtt tttggtgagg tcattactgg aatccatgca tttaaagact tggaggcggg
    77221 gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag
    77281 agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg atgcgatcat
    77341 tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga tggtcacatg
    77401 tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata actaaagaga
    77461 gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg aggtactcta
    77521 ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct gctgcgacta
    77581 acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt gtttttggca
    77641 aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct gtcgtgcttc
    77701 cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt tggaaactag
    77761 ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct tcgctgatct
    77821 ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt agctttccag
    77881 aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt tggatcttcc
    77941 cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa
    78001 gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga tcgattgctg
    78061 cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt tcttttcttt
    78121 tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta tttattgatg
    78181 tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg gaagtcattt
    78241 ctgtttccga ggcaccgttt tcagcaccag aagagcttat agttgttgaa ggttctgttt
    78301 gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata ctggaaacag
    78361 caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc cttactactt
    78421 tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca caacaggctg
    78481 tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt tccatgtccc
    78541 gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc
    78601 gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt tcaattttct
    78661 tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga ccatttcaaa
    78721 gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt ttccttgata
    78781 tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga atattgacca
    78841 gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt taaaaggatg
    78901 gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg cccaacgttt
    78961 tctttgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga taatctcagg
    79021 agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga tgtcttctgt
    79081 cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat acatcgtatt
    79141 tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc
    79201 cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac gattttggac
    79261 atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga aataattcac
    79321 atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta aaatcaattt
    79381 cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat ttttttcgat
    79441 ccgaaccaca cgaagattag cgtatttttc atcagtctag ctaatatttc tggtgtttgg
    79501 tccgttgaac catcgtctgt cactaatacc tcatagttgt gataattcaa tttcgtcatc
    79561 aaatactcga ttgtatcttc gatcacaatt tcttcattat gggctggcac catgatcgtg
    79621 atgaacggtt cgacactcaa gggaacatct acccattctt tttgcttatg tttgtataaa
    79681 aacacataac accagacacc aataaaccat acaaatccgc ctaaaatcgg ataagaaacg
    79741 agaaaaaata cataatagac aaacaagtat ctaagaataa attacctgta tgggtgattg
    79801 agatcatttc agttccaccc cattttcttt atacaaatct gcaatgaatg tcgtatctag
    79861 atttttctct tcggagacag aataaaaacg gaccgtttct ctctcttctt ttgttccaaa
    79921 acgttccgta tacacttctt caagtaattc ttgtcttttt ttcaattttt catcttcata
    79981 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
    80041 tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat
    80101 tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa
    80161 tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa
    80221 cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat
    80281 tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg
    80341 acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct
    80401 aagaaaaaga acaaaacatt tctcatcagt aaatgaaaaa tattttgttc tttttatcag
    80461 ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt
    80521 cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt
    80581 ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat
    80641 gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt
    80701 ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc
    80761 agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc
    80821 ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag
    80881 ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag
    80941 atggtagtcg tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc
    81001 ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc
    81061 taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac
    81121 atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt
    81181 acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa
    81241 ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt
    81301 ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt
    81361 accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg
    81421 actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc
    81481 ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt
    81541 ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc
    81601 taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc
    81661 tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat
    81721 ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc
    81781 acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat
    81841 cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg
    81901 catgatagca gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt
    81961 cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc
    82021 attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt
    82081 atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac
    82141 cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa
    82201 aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa
    82261 atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac
    82321 ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt
    82381 tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt
    82441 atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa
    82501 cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc
    82561 aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac
    82621 catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag
    82681 tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga
    82741 cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca
    82801 agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt
    82861 ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct
    82921 tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg
    82981 ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca
    83041 ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg
    83101 tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa
    83161 tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag
    83221 tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat
    83281 gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt
    83341 cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac
    83401 cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat
    83461 tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga
    83521 tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga
    83581 tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa
    83641 caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc
    83701 gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat
    83761 gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg
    83821 gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca
    83881 atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca
    83941 tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc
    84001 ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt
    84061 caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg
    84121 tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt
    84181 ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat
    84241 ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc
    84301 ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca
    84361 tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact
    84421 acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga
    84481 caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga
    84541 acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt
    84601 aacgctggat tttccaaaag cttttccttt aattctggga ctgttatcga ttttattgat
    84661 tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta
    84721 tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact
    84781 ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa
    84841 tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa
    84901 attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa
    84961 ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg
    85021 aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa
    85081 gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat
    85141 tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc
    85201 tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc
    85261 tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat
    85321 gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg
    85381 tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt
    85441 agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta
    85501 cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa
    85561 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
    85621 aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta
    85681 gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg
    85741 tcagctgatt gaacaccgtt taaggaagag attgtgaaaa agtcgcttag tttcgagtat
    85801 taagaaaaat tttgtgaaaa tggatttcta catttttgca taaatttggc ttaataccga
    85861 gaaactggct tttgaatacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag
    85921 tattagagaa acaaacggaa aaatagctgc tcatattttg ccgttttttg agctaatacc
    85981 gaaggtcagc tgattgaaca ccgtttacca agaggttgtg agcctaccat ttaactccga
    86041 atcacaagaa ataatttcaa aaacaacttc tcctgttttt ggtttggttg gcttattatc
    86101 gagaagtagc tactgggagt ctatttatca aaaaaaggaa tgcgacatag gtcacacccc
    86161 ttttttattt tctttgaaac atctttcggt actcgacagc atacaagaca aaagattgta
    86221 aaagaccgta agtgatgacg gcaaaaatga ctctcaccca cagcgttggt aattctggga
    86281 aaagcagcaa aaggatcatg ccaacataga tgacagcagt cgctactttg ccataccact
    86341 tcgctccact taatttgata tttttagata gcccgattaa tccagctaca aacataaagc
    86401 cttcttttat caagaataga ccaaacaaca gccaaagcca cggtcgatac catgccatag
    86461 acaaaatcag aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt
    86521 ctgttacctg attatatctc cgagcaatga ccccgtctag ataatcagac aaacctgaga
    86581 caacaatgac cgctcctgct atataaaatg actgtctcag cacaaaataa agataaatcg
    86641 gcagcaaaaa aatacggaat atcgataaaa gattcggaat cgtttgtatt tctttacgcc
    86701 agtctgtgct taatttcatt ttatcgccct acctcctcag cactcttttc tttttatttt
    86761 agcttacacg gggaataaaa ccaatgattt cactgttttt atttttcttt acagccactg
    86821 atattatgag ctaatttgag ataatcactt gctgttttca tcgtttactc ttctttcttc
    86881 ctgtcgtttg cgcatttctt ttaatagtac tttatgagct ttttttgatc ctcgctctag
    86941 gtcacgctgc cgtttacgtg ttttataatc gataaacaga ctgtctaaat cgtaacctgt
    87001 cgggtacaga tcttctgctg aacgcaccag acgaacacgc tgacgaggca cgtggattat
    87061 ttttttgtct aaatatacat caatcgtatc ttcacctata tcttcataaa ctaacgcttc
    87121 tttttggtat tcattgacaa atatccgatc accttttgca aagagctggc gttctttttt
    87181 attctccttc ctatttgtaa ctgctgtctt aggaagaaat tcttttttct gtgtccgata
    87241 ggctttttcc gccaagtaac gctctgcttg atggatcaat ttcatcgaca tatccatttt
    87301 atgagcaatc cacaaagctt ggctttctcc tgtttctcca acccgcaact gatatttagg
    87361 acgaagtgct tcccgatcga aagccatagc agccggcaca aaatcttcat gatctccggc
    87421 gaatttcttg atttctccat aatgggtcgt ggcaatgatc aatgcccctt gctcatacat
    87481 agattccatg attgcgatcg ctagtgcagc cccttcattt ggctctgttc cactgccaat
    87541 ctcatctaaa agtaccaacg tattcctttt tatctttttc aaaatagcag cgatgttttg
    87601 catatgacca gaaaatgtac tcaacgcatt ttctaaattt tggtggtcac cgacatcgac
    87661 gaaaatctca tcaaagacag caagctctgt cccttctttt gccggaactt gcataccgaa
    87721 catcgccatc aaagtcaata aaccgactgt cttcaacacg actgttttgc ctccggcatt
    87781 tgctccggtg ataatcaagc ctcgatagtc tttaccgatt tcaacatcca atggtaccgc
    87841 atgctcaggt aaaaatggat gtctgccttg cttgataatg atatgttccg atttattgat
    87901 tctaggtgtg cttccattga tttctcggct aaattttgcc cgagcaaaga tgatatctaa
    87961 caccgtgatc gtttcgatca ataaatcgat cacttcttcg ttttctgcga tagcaccagt
    88021 caatgccgcc aggacttgat attcttcact gatttcttca gcttttaaca actgatagtg
    88081 ttcattcaat ttagaaatca cagttggttc aataaaaacc gtcgttcttt tatttgattc
    88141 atcgataatc gttccatcta ttttattttt atagctggct ttgataggaa tcgtatagta
    88201 ttcgcctttt tgaacaatca ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa
    88261 gaatttcagt aattttgact ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt
    88321 acgcaaatta cgtgacgcgt cgtcactgac tttattattt tttattttct ggtcaatcaa
    88381 ctcttctaca tttatcaaat caggaagatg tttgctataa gcaaacaaca agggtgtctg
    88441 gtattgattc ttatcaaaaa actttgtgat catccgactg cttctaagaa aatccgcata
    88501 ttcgatcaaa tcggttggct gcagaatcaa cccttttttg acttgttctg tcagtgcatc
    88561 gatcctcggc aaccccataa atggaacatg ctgactgctt tctaagatca agcgtgcttc
    88621 tcttgtttct tcttgacgat ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct
    88681 gattcgtttt ttgctatagt ttccaattgc tcttgcttgg atttcctttt tgatccgatc
    88741 aaattgtaat tgactaatca tttcgttgtc catattctct ccttttcttg aagagatacc
    88801 tgtcccaaaa taaataaggg cctagagata aataaaagga tagaacacag cttaaaaaag
    88861 tcatgtccta tccttattag aatacaaaga tattgttttc ctgacaggta ccgtttcata
    88921 agcacaccaa ataaactttg tttattggct attcgactta ctgattagat accagctaca
    88981 ctcacaaaac ataccccatt cttctaggtc ttatttattt tccacttaac tatagcaaga
    89041 cacattttgg ttgtcaaact ttagtatgtt ttatagattt acaccattaa tgaatagaca
    89101 cctaccgcga taattgctcc gatcattgga cctgcgattg gtacccatga atatgcccaa
    89161 tctgaagttc ctttattagc tattggcaat accgcatgtg ctaatcgagg accaaagtcc
    89221 cgagcagggt tgattgcata tcctgttggc cctcctaatg aaagaccgat ggctaagatc
    89281 aatccaccta cagcgaatac gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc
    89341 aacccaagaa ctaatacaaa tgtaccaatt aattctgtga tcacatttgc tggataattc
    89401 cgtatagcag gacctgttgc aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc
    89461 caatgtggca aataagaaag ccaaacaaga atggcccctg caaaagctcc taaaacttgc
    89521 gcaatgatga aaggaacgac aagattccaa ctaattgccc ctgtcatcgc catagctaga
    89581 gagactgcag gattcaaatg agctgggccc atgaatccag aaatatagac agccattgtt
    89641 acagcgagtc cccaaccaaa tgcgatgaca atccagccag aagcctgtgc tttacttttg
    89701 tttaaattca cagccgcaca cacaccatct ccaagcaaaa ctaggatcag tgtcccaata
    89761 aactccccca tgatttgagt catatcactg ttcatttatt tctccccctt tagttcccgc
    89821 agatctgatt ctgcaatcaa tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct
    89881 gtccattcaa aatatccagc aattgcatcg atcacaggtt gcttgatctc atcaagctga
    89941 tctcgttcaa acaacagatg atttgtacga cgaatgagat aatcgcctgg ggccaaaacc
    90001 atttcttctt ctaaaccata acgtaaccga gctgactcag cgagactcaa accaggataa
    90061 ggtgccattt cttttgctaa ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg
    90121 atataagtag catcttcttc ctctaaaccg gctgctactc cttcttttgc taattctgtc
    90181 actgtttctt ctaatttagt tggatcgaag tttccacctg aaatctgata ttttttagag
    90241 tcgatttctt tcgtctctat tccgtattct tcttttaata attggcgtat caatcgcaaa
    90301 gctccttctg ccatcttacg gtaatcggtg attttcccac cagataaagt cagcaaacca
    90361 tctggttctc tttctaaaga actacctctg gaaatcgtag aaggtgcttt ttcatcacgg
    90421 ctgttttcca aatggttcaa cacgtcttct acttcagcac gagaaacttt gttttcctta
    90481 tattcactta ctgtatcgac aactttattg aagctcttgt ctgaaatcga tccattatct
    90541 ccaccattgt aatcagaacc agaattacca atcaacagtg gacgaagtcc tgcccagctt
    90601 gcttcgatat cattcaatgt gatatttgct tctggatagc gatgattgat cacatccaat
    90661 agataatcta catcttcttg tgtgactttc ggatccgtaa aatctccttg gtaatccgta
    90721 tctgtcgtac caaagtaagt cttgttttct cttggaatag caaaaaccat ccgcccatct
    90781 tgctttcctg tatcgaagta tgtgggttgc ggtacaggca gttttttcgc atctacaact
    90841 aaatggatcc ctttagttgg acgcatttta ggagagactg gacgcgtaaa attcaagttt
    90901 cgtactttat ctacccaagg accactcgta ttgatcacta attttgcttt gatttcgatc
    90961 acttcatctg tcagcaggtc acgagctttg acgccaacga tttgatcgcc atcatataaa
    91021 aagccaaccg ctttcatttt acttactaga taagccccat cttcggcagc ctttttgata
    91081 ttatcaatca ctaaacgggc atcattgttg cggaaatcta gatagacacc cgcgcctttt
    91141 agcccttctt ttttcaaaaa cggttctctt tccaatactt cttctggtgt cagcgtataa
    91201 ttctcatatt tggttcctgt cacatttgcc agtttgtcat aaaggtccat tgctactttg
    91261 accgagaaca tattgaaggt tgttgctcct tcgccctcat agattggcaa aagcattgga
    91321 tctggttttg ggatgtgtgg ggcaatactt tgtacgactg cacgttcacc aactgtgtca
    91381 gctactactt ccacatcaaa tgtcttcaga taacgaatac cgccatgaac aagtttggtc
    91441 gagcgagagg aagtcccttc tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct
    91501 gatgctgctg actggactgc cacaccggca ccagtgattc cgccaccgat gatcaacaga
    91561 tccaattctt cttgctgcat tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata
    91621 tggatttcct ccttattcaa atgcttgggt agcttttact gcttttttcc aaccgttgta
    91681 aagtttttct cggcgctctt cctccatttg aacttcaaat agcttgccag cttcataaaa
    91741 ttcgcggatc tcgtctgtat ctttccaata accgacagct aatcctgcta aaaatgctgc
    91801 ccctagcgcc gttgtttcca agttttctgc tcgttggatc ggtacattca agatatctgt
    91861 ttggaattgc atcagatatt cattgtttgc tgcaccgcca tctactttca atacaggaat
    91921 cttgattcca gtgtcatctt gcatcgtatc gatgatatct ctcacttgat aagcaatcga
    91981 ctgtaaagta gctttaatga tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc
    92041 ccctcgtgct gcctgatccc aataaggagc acctagccca acgaatgcag gaacaacata
    92101 aacctcatct tcgcttgtag agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa
    92161 catttgaagt ccatctctca gccattgaat cgaagaacca gccacaaaaa tacttccttc
    92221 taaagcatag taaacctttc catttatgcc gtaaccaatt gtcgtcaata aattattttt
    92281 ggataattgc ggtttttcac cggtattcat gacaataaat gaacctgtac cataagtatt
    92341 cttgaccatg cctggttcga atgccatttg tccgaataat gcggcttgct ggtctcctgc
    92401 catccctgaa attggtgctt cgcttccata aaaatgataa ccttgcgtat aaccataaac
    92461 ttctgagtta gaagtgactt ttggtaacat cactcgagga atatttagta aatctaaaat
    92521 ctcctgatcc caatctagct gatggatatt gaacaacatc gtccgactgg cattcgagta
    92581 atcagtaaca tgtgcttgtc catcggtcag tttccatact aaccaagaat cgatcgttcc
    92641 aaacatcaat tcgccttttt cagctcgttc ttgcgcgcct tctacatgat caagaatcca
    92701 gcgtatcttc gttgctgaga aataggcatc gatcactaac cctgtttttt cgtggaaaaa
    92761 ttcttgatag ccttcatttt ttaagttatc tgcaattcca gctgactgtc tggattgcca
    92821 aacaactgca tgatagattg gacgtcctgt atctttttcc catattactg ttgtttcacg
    92881 ctggttcgtt atgccaatcc cagcaatctg atttggtttg atccctgatt cgatgaacgc
    92941 tccggcaata actgactgta cagaattcca gatttcattt gcatcatgtt ccacccaact
    93001 ttcttgcggg aaatattgcg tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt
    93061 gtcaaagata attgctcgtg agctagttgt accttggtca atcgccatga tatacgttga
    93121 ttctgtcatt gtgattccct ccattatatg caaacgcttt ctttaactac tcttatcata
    93181 ctactgtttt aaaaaaagaa actgtcaaaa ttttattctt tttttctttt tttgacagtt
    93241 ttttattatg aagctatttt ttaatcaatt cttttctttt ttcagcgtct ctattcttct
    93301 tttcaatcga ataatgtcgt aaggtgacgc aaattctgac agataatatt cacactgttt
    93361 tttgtttctc tggcaaaatg tgatcaccaa atcatattct ttaccttctt cataacttcc
    93421 aatctcaata ccgccgattc ccctcagttc tctgatcaga tattgttgaa aagcaatacg
    93481 ataaatcggc agactttcta aatcgattcc aatcactact ggttttgcaa tataaaaatc
    93541 gatcatcaaa agaacattgg catactggat catcaggtaa ctaagctcac tatcttcagc
    93601 tcttttcctc ggcaattgct gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa
    93661 ggaatgcccg agcattttct gctgctgttc caaaagccga tcacgctcat agacttcgat
    93721 ctttccaaca aagaaaaacc attcattgtt gatctgggaa atttggtagc ctactgcctt
    93781 ttcttctttg attttcaaac gacgaggacg ataatgccat aacatcgttt cccgaatgta
    93841 agtatctaaa acagcggttg gtaacttttt gctccgtgtc aaatcatact gataaaatat
    93901 ttcttcgtct actatcgaaa aagaaacaaa aaagctgtaa aacatcattg tttcgtatac
    93961 attcagttct gctgctgttc gactaagatg catcacgatg attggatcga ttgcttgata
    94021 aagccggtcc gactgataga gtaatttctt ttctttcaaa gttgcgtacg tacttttttc
    94081 attcaatagt ctttttttcg taatcccgag ccagcaagca atctgtgctt ctgctgatgc
    94141 cgtaaacgtt gtttctaata cacggtccaa tcctagaatg aacggtcttt tttctggtgt
    94201 attttgcaaa aaaagcggtc gctgatcttc cgggatatat tggaatagtt cataataaaa
    94261 atatcgtatc tgtagctctt ctccgtataa ttgaccgttt ttgatttgta atccaaattc
    94321 ctctaagagc tggttcaatt ctttgatctt tcgaaaaaca gaagactcac tgatattaaa
    94381 cgctgtcgct aatcgaacga tggatacttg ctgatgttct aataaataga cgagcatctt
    94441 aaacttcaag ctttcctgca ataaaaacaa tagaattttt tccaaactta catcttcgtc
    94501 caacacaagc agcgcctgat actcatttcg ctgtacttct actttttttt ccatcattcg
    94561 gccaatcaat tcaatatctt ccaaatattg atcaaacgag gcttttgaca gttggacatg
    94621 ctctctcaaa ccattgatag aggctgttcc cccttgcaga acaaggtttc gcaaaatcag
    94681 gatctgtaac tgctcttttc tttctaataa tgcttcaatc ttcataggca gtattcgcca
    94741 cctttgacca caccatatct ggcacacggt attctggctg gaaaaatagc tcatgttctt
    94801 ctaactgttc aaacgtccat ccttctttta ctgcaattgt cagttggtca agaaggtgga
    94861 acatctcttt tttgctttgg agctgtgctc caagaatctt tttttcagaa tcaaatagaa
    94921 atttgagttt aactttgctt tgatttattg ctgtaagctt gtagtctacg tatcctgttt
    94981 caattttttg gggataaaaa atgctctctt cttctgtcaa acctacgctt cctaagaaat
    95041 agccaaacca ctgattgccg actggacgat aggttccctg atcctttgtc tgtgagccac
    95101 taattgtctt gctgacattt cgtgctgtac ggacagcgtt atttactaga gaaacataca
    95161 ttttttcatt tgttggacga aaagttactt ggatcgcatc gccaatcgca taaacgtctt
    95221 tttgagacgt ttgcaagtag tcatctaccc agatcgttcc atcatcattc aatgttaatt
    95281 gttcttccca catctgattg tcggggcgag aatgattagc cagcaaaaca agatcatagt
    95341 tctcctttct tttttccgtc tctacaatag cggtttcatt ttcggtcaaa gacacaactt
    95401 gttcttctaa aaaaagtgaa agcccttgat ttttcatttc ctttactaac ggttctgtca
    95461 tttcaggatc tagataacga aaaagaattg actttcggct ttcgtaaaga tggatttgtt
    95521 tttttcttga tgccaaccct tcagctattt cgattcctgc ttgtcctgca ccaataattg
    95581 caattttctt tgcattcatg atcttttgct gaagagccgt tacgttttgt atctttttaa
    95641 ctgggtgaat aagcgttgat tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg
    95701 ctagaatcaa acgatcaaaa tccaattttt ttcctcctgc taaaacgact tttttttctt
    95761 tcaatcctat tactgtcttt tctgtgtaaa catggattcg atagcggtca gtcagttctt
    95821 ctttcgtgat ccaagaaagt tgtttttcac tggtaacctt tccttttaat atcaaattga
    95881 acccgctggg aatccatcct aacttcgatt gcttttcaat caaatatatt tcacttgttg
    95941 gcatcaattg gcgaagataa atagccgcat gaatccctgc aaacgacccg ccgacaatta
    96001 caatgttcat ctgcattcct ctttcttata aggtgttcta ctgctaactt ctcaatcaga
    96061 aaacacagta ttcataactc gctttcaacc ttatgataca tagcgatgca gcagaatcaa
    96121 ccctctccat cataccgtgt tctaggtctg cttctcaata atgtactagt ggacctaatt
    96181 gaagagtatc atttctgaag agatgtttca aaaaatgtac tcttataaaa gaaaaaaact
    96241 cgccttttac gtacctctgt ccaaatagga aacagaaaag aacgaaaaga cgagttttag
    96301 cgtgtgtaat gtaatagtta gaatagacac atcatttatt tttaattctt taatgcttca
    96361 acaactgctt cgcctaattg atgagcagat tgaggatttt gccctgtaag aagccgccca
    96421 tcaatttgaa ccacttctga aaaagctgct ccagactgat agtttgctcc tttttctttc
    96481 aaagcatctt ctgctaagaa tggtacttca ttcgttgtac catttgcttc ctcttcttca
    96541 ttactgaacc cagtcactgt acgtccagct aaaatagatt ttccagactc atctgtaact
    96601 ggcaacaatc ctacaacacc gtgacaaaca gctgagacca gcccttcatt tcgatagatt
    96661 tgtgaagcga ctttcccaag atctattgct tctggaaaat cccacattgt tccgtggcca
    96721 ccagcataat aaaccgctgc ataatcattt gcatccacat catcaggacg taaggtattt
    96781 cccaaggccc gatttctgaa atcttcatct tcataatagg cccaatcgat ttcatccata
    96841 ttcaaaacac tttgcggttc taaaggtaca tatccgcctt taggactgac aaaatctgca
    96901 tcgatcccat tttttactaa tacatcataa aaatgagtca gttcacttaa ccacaaacct
    96961 gtaggacgct cgatcttttc gtacttttct acatttgtta atacgatcaa tactttcatt
    97021 ttcccatcct ctttctataa aaattgcgtt attgataacg tgctttcaag aaattatata
    97081 atgggcttgg caaataaagc tctttatatg cttcttcaaa aggtttcgtt acaaattcat
    97141 cctcacggtt ttccaataat ttctctgccc agtcaggatc gacgatcatt tgctgtccaa
    97201 ttgctgcaag ttctgcatat ttcaatacct gctctacatc ttcgcgattg cggatcccac
    97261 cgacaccaac taaagggatc tttccttgaa tcgtgttatg gaccacttca agaatcgatt
    97321 tatcgttgta tttatcagaa cgagctattc gatcatacat atttaatgaa acatgcagat
    97381 aatctagttt tgactcacgt aatcgttcaa gaagccagat cgtgtcttca aaacggatac
    97441 ctggtgtttc atattcttct ggtgagaaac gataccctac gatgaatggt tttttagcat
    97501 attgttcaac cgctgagaaa accgttgtta ataattcttc aataaaacga taacgatttt
    97561 ccaagctacc gccccattga tcctctcgtc tattcgaatg cggagagaaa aattgttgga
    97621 tcaaatacgt atttgctcca tgcaactcga ttccatcaaa tcctgattca atcgctctac
    97681 gtgtggcttt gccaaatgct tggattgttt caacgatttc ttcttcactc attgctcgtg
    97741 gtgtttccgc atcaggacgt tttgctgcaa ctgcacttgc agatacaggc tgttctccag
    97801 ataaagttgc tcgacttgtc attcgtccac cgtggaaaat ctgaataatc gcttttgcgc
    97861 cttctccttg gattgctttc gctagctcac ttaatcgagg aatcatttca tcatgagcga
    97921 tgctgagttc acctggccag ccttttccga tatcttggac atttgcagca ccagtaataa
    97981 cagcaccaag acctttagaa cgttgtttat aataagtgat ctcatcttcg gtgatcactc
    98041 cattgaaaaa gctgagttgt gtcgtcatag gcgacagcat caatcggttc ttcaattgaa
    98101 ctcctgaagg taaagtaatt gattgatcaa attgtgacat tgtcattcct cccataattc
    98161 gctctgatta acctatcgag aatcatctat cattaaacca ctcgattagt tgtacatgca
    98221 actaatttgc ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc attcattgtc
    98281 tgggctaatt ctttacttgc ttcatttccc aaaactgctt tgtatgactg atgaagttct
    98341 tcccagctac catcgacttc cttagcaaat tggcgtcctt gttctgtaag ataaactaat
    98401 gacaagcgac cttcgctaac tgttttgacg tatcctttat attctaattt ttcaacaaaa
    98461 cgagcaatcg ttgatggggc ggaatagagc atttgtgcaa tatctttttg tggtattcca
    98521 tctttttctt ttaatatcaa taaaataaaa ccataagttg gtgccagacc tgtccttcta
    98581 aacgcttcat cagctaattt attcaacatg cgatctagct ttttcacgct aaagaaaaga
    98641 cattcatcta acgctttttc aattggcttt tccatttcat cccctccatt ggcgtacata
    98701 tcatagtatg atttttattt tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct
    98761 agatttgaaa aaaagaagta gccatcttca aagaatttcg gcttgttttc gacaaaactc
    98821 cttctggaac accgtgaatc agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa
    98881 aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata ccgaaaaact
    98941 ggcttttgaa caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa
    99001 gaacagccaa acaaaaatag ttcctcatat tttgtcgttt tttgagctaa tgccgatggt
    99061 cagctgattg tacaccgttt attgagaggt cgtgagcctg ccgttgaact ccgagtcaca
    99121 aggagcaatt tcaaaaaaca gcttctcatg ttttatgact atggtgactt gtgatcgagg
    99181 agttggcagg agaatactgt ttattcgtaa agaaggggct gtgacaagac ttttgtcaca
    99241 gtcccttatt tccttttatt ataggatttc gccatttgat tggatgactt gtctgtacca
    99301 atcaaatgat tttttcttta accgtttctt tgtgccattc ccttcgtcat cttgatcaac
    99361 atagatatat ccataacgtt ttgacatttg tgaagtagaa gcgctgacga tatcaatgca
    99421 gccccacgac gtataaccaa ataaatctac tccatcttcg atagcttcct tcatctgctc
    99481 aatatgctgc ttcaaatagt caatgcggta atcgtcttga acggttccat cttcttctac
    99541 tttgtctata gctccgagtc cgttttcagc tatgaacagt ggtttttgat acctatcata
    99601 taattgattc aaagcaattc tcaaaccctt aggatcaatc tgccagcccc attcacttga
    99661 tggaagatac ggatttttaa ttcctccagt tattaagttt ccttggaccc cttccaaatt
    99721 ttctggatga gcactgactg cactagacat gtagtagcta aaagaaacaa aatcaacagg
    99781 gtaggctgct aagatttctt catcacctac ttctattttt aagttgacac ctttttcttc
    99841 aaacattcgt tgcgtatacg ctggataata tcctcttgct tgaacatcac taaagaacaa
    99901 agaagttctt tgctgctcaa ttgttttttg aatatcatcc ggatcacaag aatacggaac
    99961 aagaagctga tagctgatca tacaaccgac ttgtgaacct ttgataattt catgacaagc
   100021 ttttgtagct aaagcacttg ctataaactg atggtgtgca ccttggtatt tcgcttgttc
   100081 caaatgggga tggttttctt caattactcc tacgctaaca aaagggtgat gttttacaca
   100141 gttgatctca ttgaaagtca tccaatattt tactttattt ttatagcgtt tgaatactgt
   100201 ttcagcgaaa cgaacgtaaa actgaataag ctttcgatta ggccatccac catattctgt
   100261 aaccaaatat aacggcattt cataatggga aagagaaacg actggctcaa tcccatattt
   100321 tagacattcg tcaaacactt gatcataaaa aaataatccc gcttcattgg gtgtttcttc
   100381 atcaccatga ggaaaaattc ttgtccatgc gatagacatc cgatagcatt taaaacccat
   100441 ttcagcaaat aaacggatgt cttctttaaa gtgatgatag aaatcatttc cgtgtctttt
   100501 aggataataa tacttgctgt tgggatcctg cgccgctttt acttgctgat gcgtcatcat
   100561 atgccattga tctgtccatt tttctttagg taaatcgctg tcgaatgtat acacatcaga
   100621 cacagataat cctttttcat ctacatccca tgcaccttca cattgattgg ccgctgtcgc
   100681 tccgccccat aaaaatcctt ctggaaattt tttcatatcg atccccctaa acttgctagt
   100741 tttcttgttt gattattttt cttatgacta gtaatgatat ttttgaatct actactattt
   100801 gtaacaataa ccattgtggt cggatcatat ccctctttga ttaattcttg caaatctact
   100861 tccatcaata aatcgccttt acgtactttt tttccatcag atactgcact ttgaaagtat
   100921 ttgccattta aattcactgt atcaataccg atatggatca gtatctctgc tccttcttca
   100981 ctgactaccc caattgcatg tttcgttgga aaaactgttg aaacttcccc gtttacagga
   101041 ctgtagattt ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga tccactagca
   101101 aatgttggat cgtttacttc agataaatca attatttcac ctgctgcagg agtacttaac
   101161 actatatcct ttgaggattc ttcttcgatt tcatccttgt agccgaaaag aaaagttagc
   101221 atacaggaac tgatcatcgc taagaaataa ctagcaacta gccaaacaaa tccttcacca
   101281 atataaatcg ggaaagacaa aatacttctt gtagcaactg ccaaagcacc ggctcctgcg
   101341 gcaccagcta ctccaccaaa tatagctcca catatacaag caatgtaaaa tggtcgttta
   101401 tatttaagag tcacaccata aacagcaggt tcagtgattc caaataatgc tgtgataaat
   101461 gcagataaag ataaactttt tactttctta tttttcgttt tcaaaaatac cccaagtact
   101521 gctcctgctt gcgcagctac ggcagtcata catgctggac ctagcgtatc acgaccgtag
   101581 aaagaaagat tgttggtcat tactggtaca agtccccaat gtaacccaaa aatcactaac
   101641 acttgccaaa atccgccgat aacagcacca gataaaacag ggctaaagtt ataaacagct
   101701 aaatatccag ctgcaatcag ctgacttgca taatctgcaa ttggacctac aactaataaa
   101761 gtaattggga ccatcacgat gatacaaatg aatggtgtca ataaatttcg aatactttcg
   101821 tgcaagcttt tgcgaatctt aggctcaata taagaaagaa cccatacagc taaaattgct
   101881 ggaataaccg tacttgtata tcttgctaga atcacgggca tccctagaaa tcgcaaggtt
   101941 atgctatcac tatatgcact gacaatcgta ggataaacga gagctgctgc aataaccata
   102001 gcgatgaaac gatctgtatt gaactttttt gcagcagtat atgccaaaaa tataggcaag
   102061 aaatagaaaa atgcatcagc tgctgcattc aaaatgatat aagttcctga ttcagtagtt
   102121 aataaattca aactcgtaca taaggcgagt actcctttga tcaaaccact tcctgccaag
   102181 acgcctaaag tcggggcaaa aacagcggcg atcatgtcta taaaagagtt taaaatgcct
   102241 tttttctcag ttcctctctt tccactagat gcatcatctt ctagtttcat ttgtttggat
   102301 aatacgtcat acacttcatt tactgcattt cctactacga cttgaaacat tcctccagct
   102361 tcgactacag tgatcacacc ttctagttgc tctaccttct ccttatctgc cttcttatta
   102421 tcttttaggg taaagcgtaa ccttgttgca caatggtaaa cattgctgac attttcttcg
   102481 cctccgacaa atcggacaat atctttcgct aattgtgaat agtccatttt tacttccccc
   102541 ttgttaatta atgccgtagc aatccttctt acaagataag tatagttctt agaaaacggt
   102601 ttctccattc aattagtacc ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg
   102661 tttacttagc cgagcaaatc actcactgct tgaaagaaaa caagtatatc gtttctctta
   102721 tagtcaaagg agacaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc
   102781 tcaagctgat actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa
   102841 gtctcggcat ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg
   102901 gaatctatct ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt
   102961 tttgttttct ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc
   103021 gaagattcaa actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata
   103081 agctcttgct cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg
   103141 aaaagataca ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc
   103201 tgttttttta gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct
   103261 tgttttcgaa aaggattcaa cgcttcggca agatacaaaa ccaaataagc gctttcagct
   103321 aaattgggac gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa
   103381 ggataacggg agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc
   103441 ctcgttttca tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa
   103501 acaggaagat ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt cttctctatt
   103561 tctttgtttt tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt
   103621 gcttgttttt cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg
   103681 gtctctacca tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt
   103741 cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata
   103801 taattatatg atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa
   103861 aaaattgtac tgatttgttg aggcaacctt gcgtgttgga tcaccgtttc tgttccaaca
   103921 agagataaga aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac
   103981 gaaacttcca gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt
   104041 aatgatacgg ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac
   104101 cctttttgaa acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag
   104161 attttatgtt tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct
   104221 aaaaaataac ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc
   104281 atatttttga tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct
   104341 tcgcttgtta tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta
   104401 tttaagtcca tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa
   104461 tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt
   104521 tttaccatta gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct
   104581 aaagcttccg ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat
   104641 ttcccctgtt cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg
   104701 gcaaccatct tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg
   104761 ttttggatcg tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc
   104821 aactatgaat aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc
   104881 tagaggatca attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc
   104941 gaaaccattt gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct
   105001 acgataataa ctaaatgcaa tataatctac cgtgttagct tgcaagtact gttcgtctcc
   105061 atctaacatt actggtgcac agtcttcttc cttccatatc ctgtaaatat aagctggata
   105121 atgccctcgg cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc
   105181 aaatacattc tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat
   105241 aatacccatc ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc
   105301 tagaaacata tgatgacagg cttgataaat ctcttttttt gttagtgtag caatgggttc
   105361 aagtttatct ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc
   105421 attattgatc tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac
   105481 ggtctgcgca taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc
   105541 tcctaccaat gctaaggggg tttcataatg actaagagta attactggct cgatatcata
   105601 tcgatttaat gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg
   105661 ttcgtctcct cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc
   105721 taactccttc agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg
   105781 agaaagataa tggagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca
   105841 ctgaatacta ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt
   105901 gccgtcttcc aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa
   105961 attttctgga aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac
   106021 ttgaagtata gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga
   106081 aaaaaattga atgctactct tcatttttaa tatttatata taacaaatac ataagacgat
   106141 ccccgaaaga aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa
   106201 atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg
   106261 acacaataac ccttaaagct tcacgtggaa cagtgaacgt ctgcttttca ctggacatat
   106321 tacaaataac gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag
   106381 gcgtcttttc cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat
   106441 cgatcaattt cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat
   106501 tgatttcttt ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat
   106561 gttcacgggc gttccattgc ataggcgtcc tagcattgtc tcttcctttc gcattgattg
   106621 attccaagat ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg
   106681 tctcaatatc ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt
   106741 ctccttgata gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg
   106801 cactttcttt tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt
   106861 gattattcca aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata
   106921 atactttttt cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt
   106981 gctgatctaa tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat
   107041 tggaataaag tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat
   107101 ttttgtcacc aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat
   107161 tacttgtgat ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc
   107221 caccaatacc tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt
   107281 tatttttcca attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg
   107341 ttttttcatc caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt
   107401 ttccattgac cggatcacgc caaatataat agtcccgata aggattttct ttatttttac
   107461 gggcctcaat gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga
   107521 tacggatatt tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga
   107581 attctgccat gattgcttga taattactga tatcataccc attgtcatca ttcggagact
   107641 cgtatactgg agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg
   107701 aaataatccc ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat
   107761 agatttgata aacaactgaa tggtgccacc atttttgttc catgattttc ctcctataaa
   107821 tggatgtttc tattttttgc ttaatcattt attttagtct ctctcactaa aaattgtaac
   107881 gctttcaaat agctattgca ccttgttatc ggtaacagaa taaaacaaaa aaacagctga
   107941 ggaaattccc tcaactgttt tttgaaagga ttgatttatt cagggaagaa tgctgatttc
   108001 acaacttctt tataaacggt gttcactcag ctgtccctcg gtattagctc aaaaaacggc
   108061 aaaatataag aaactatttt tttcgttttt ttctctaata ctcaggccag aacactgatt
   108121 tcacaacctc tttataaacg gtgttcactc agctgtccct tggtattagc tcaaaaaacg
   108181 gcaaaatatg aggaactatt ttttcgtttt tttctctaat actcaggcca gaacactgat
   108241 ttcacaacct ctttatttca cattcaatat ccacatgaat cccatgaata caaagaacag
   108301 accatacatg atgggagata cttcttttcc tcttttagct gctaacatgg taattggata
   108361 gaagataaaa cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa
   108421 gatcaagaat gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg
   108481 tgccatcaat actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga
   108541 tagtaatgga gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat
   108601 acctgaacgt cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga
   108661 agtacctaat aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac
   108721 acgaggcatc ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc
   108781 tgctgtatca aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga
   108841 attgatgtcc cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc
   108901 ggcaacaatt tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc
   108961 tgccatcccg atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac
   109021 taatccaaaa atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt
   109081 agattcattc gaaacgatga tcccaccttc gctcagacca aggaaagcaa taaatagacc
   109141 gataccgcca gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg
   109201 cagcttgaag attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc
   109261 tgtttcccat ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc
   109321 aagagcaggt gccgttccaa ttggataacg ggctaatacc cccattaaaa tacagccgat
   109381 cgcactggct aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa
   109441 tacagttgga tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag
   109501 tacctcacgt tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc
   109561 cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt
   109621 tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt
   109681 cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat
   109741 gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta
   109801 gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga
   109861 attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt
   109921 ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac
   109981 tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt
   110041 ctcctccttt ttattcaatc agacgactca gcttttccta taacaaaata attgccttca
   110101 gggtccgaaa aattgaaagt caagacgcca ttgttgtctg tgatttctcc tggttcttcc
   110161 atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct
   110221 aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct
   110281 tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt
   110341 tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc
   110401 atcacacgtg ttttttgtat catcgtttga cccttctttc tatttgattt tctttcctgt
   110461 ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa
   110521 gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt ta
//
LOCUS       DAPBAB010000004        99769 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPBAB010000004 DAPBAB010000000
VERSION     DAPBAB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 99769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.004"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(554..2167)
                     /locus_tag="RD444_000357"
     CDS             complement(554..2167)
                     /locus_tag="RD444_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7547762.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="RD444_000358"
     CDS             complement(2308..3216)
                     /locus_tag="RD444_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7547763.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHRSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="RD444_000359"
     CDS             3399..4523
                     /locus_tag="RD444_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7547764.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4548..4727
                     /locus_tag="RD444_000360"
     CDS             4548..4727
                     /locus_tag="RD444_000360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547765.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4750..5667
                     /locus_tag="RD444_000361"
     CDS             4750..5667
                     /locus_tag="RD444_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7547766.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="RD444_000362"
     CDS             5660..6493
                     /locus_tag="RD444_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7547767.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="RD444_000363"
     CDS             6576..7694
                     /locus_tag="RD444_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7547768.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7688..9586
                     /locus_tag="RD444_000364"
     CDS             7688..9586
                     /locus_tag="RD444_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDT7547769.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9580..10803
                     /locus_tag="RD444_000365"
     CDS             9580..10803
                     /locus_tag="RD444_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7547770.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="RD444_000366"
     CDS             complement(10889..12823)
                     /locus_tag="RD444_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7547771.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13049..13705
                     /locus_tag="RD444_000367"
     CDS             13049..13705
                     /locus_tag="RD444_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDT7547772.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13779..14132)
                     /locus_tag="RD444_000368"
     CDS             complement(13779..14132)
                     /locus_tag="RD444_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDT7547773.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14324..15253
                     /locus_tag="RD444_000369"
     CDS             14324..15253
                     /locus_tag="RD444_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDT7547774.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15264..16100
                     /locus_tag="RD444_000370"
     CDS             15264..16100
                     /locus_tag="RD444_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDT7547775.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16348..17124
                     /locus_tag="RD444_000371"
     CDS             16348..17124
                     /locus_tag="RD444_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7547776.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17300..18043
                     /locus_tag="RD444_000372"
     CDS             17300..18043
                     /locus_tag="RD444_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547777.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18036..19616
                     /locus_tag="RD444_000373"
     CDS             18036..19616
                     /locus_tag="RD444_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDT7547778.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19867..20346)
                     /locus_tag="RD444_000374"
     CDS             complement(19867..20346)
                     /locus_tag="RD444_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDT7547779.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20362..21114)
                     /locus_tag="RD444_000375"
     CDS             complement(20362..21114)
                     /locus_tag="RD444_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDT7547780.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21231..21301
                     /locus_tag="RD444_000376"
     tRNA            21231..21301
                     /locus_tag="RD444_000376"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21263..21265,aa:Gly,seq:ccc)
     gene            21662..22387
                     /locus_tag="RD444_000377"
     CDS             21662..22387
                     /locus_tag="RD444_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDT7547781.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22642..22908
                     /locus_tag="RD444_000378"
     CDS             22642..22908
                     /locus_tag="RD444_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDT7547782.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22905..23336
                     /gene="ruvX"
                     /locus_tag="RD444_000379"
     CDS             22905..23336
                     /gene="ruvX"
                     /locus_tag="RD444_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDT7547783.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23361..23666
                     /locus_tag="RD444_000380"
     CDS             23361..23666
                     /locus_tag="RD444_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDT7547784.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23747..24892)
                     /locus_tag="RD444_000381"
     CDS             complement(23747..24892)
                     /locus_tag="RD444_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7547785.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24953..25600)
                     /locus_tag="RD444_000382"
     CDS             complement(24953..25600)
                     /locus_tag="RD444_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7547786.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25794..26087
                     /locus_tag="RD444_000383"
     CDS             25794..26087
                     /locus_tag="RD444_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547787.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26131..26442
                     /locus_tag="RD444_000384"
     CDS             26131..26442
                     /locus_tag="RD444_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547788.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26439..27063
                     /locus_tag="RD444_000385"
                     /pseudo
     CDS             26439..27063
                     /locus_tag="RD444_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27204..27362
                     /locus_tag="RD444_000386"
     CDS             27204..27362
                     /locus_tag="RD444_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547789.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27363..27725
                     /locus_tag="RD444_000387"
     CDS             27363..27725
                     /locus_tag="RD444_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547790.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27762..28610
                     /locus_tag="RD444_000388"
     CDS             27762..28610
                     /locus_tag="RD444_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDT7547791.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28600..30075
                     /locus_tag="RD444_000389"
     CDS             28600..30075
                     /locus_tag="RD444_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDT7547792.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30341..30748
                     /locus_tag="RD444_000390"
     CDS             30341..30748
                     /locus_tag="RD444_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDT7547793.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30751..30966
                     /locus_tag="RD444_000391"
     CDS             30751..30966
                     /locus_tag="RD444_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547794.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30970..31350
                     /locus_tag="RD444_000392"
     CDS             30970..31350
                     /locus_tag="RD444_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDT7547795.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31484..31639
                     /locus_tag="RD444_000393"
     CDS             31484..31639
                     /locus_tag="RD444_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDT7547796.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31708..32181
                     /locus_tag="RD444_000394"
     CDS             31708..32181
                     /locus_tag="RD444_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDT7547797.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32178..33872
                     /locus_tag="RD444_000395"
     CDS             32178..33872
                     /locus_tag="RD444_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDT7547798.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33838..34023
                     /locus_tag="RD444_000396"
     CDS             33838..34023
                     /locus_tag="RD444_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547799.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34027..35202
                     /locus_tag="RD444_000397"
     CDS             34027..35202
                     /locus_tag="RD444_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7547800.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35195..36718
                     /locus_tag="RD444_000398"
     CDS             35195..36718
                     /locus_tag="RD444_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDT7547801.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36774..37058
                     /locus_tag="RD444_000399"
     CDS             36774..37058
                     /locus_tag="RD444_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDT7547802.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37045..37380
                     /locus_tag="RD444_000400"
     CDS             37045..37380
                     /locus_tag="RD444_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDT7547803.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37675..38301
                     /locus_tag="RD444_000401"
     CDS             37675..38301
                     /locus_tag="RD444_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547804.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39043..39879
                     /locus_tag="RD444_000402"
     CDS             39043..39879
                     /locus_tag="RD444_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDT7547805.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39944..40378
                     /locus_tag="RD444_000403"
     CDS             39944..40378
                     /locus_tag="RD444_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7547806.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40446..41768)
                     /locus_tag="RD444_000404"
     CDS             complement(40446..41768)
                     /locus_tag="RD444_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDT7547807.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41922..43562)
                     /locus_tag="RD444_000405"
     CDS             complement(41922..43562)
                     /locus_tag="RD444_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDT7547808.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43892..44362
                     /locus_tag="RD444_000406"
     CDS             43892..44362
                     /locus_tag="RD444_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDT7547809.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44991..46094
                     /locus_tag="RD444_000407"
     CDS             44991..46094
                     /locus_tag="RD444_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDT7547810.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46378..47058
                     /locus_tag="RD444_000408"
     CDS             46378..47058
                     /locus_tag="RD444_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7547811.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47148..48182
                     /locus_tag="RD444_000409"
     CDS             47148..48182
                     /locus_tag="RD444_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDT7547812.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48196..48531
                     /locus_tag="RD444_000410"
     CDS             48196..48531
                     /locus_tag="RD444_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547813.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48515..50995
                     /locus_tag="RD444_000411"
     CDS             48515..50995
                     /locus_tag="RD444_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547814.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51051..52610
                     /locus_tag="RD444_000412"
     CDS             51051..52610
                     /locus_tag="RD444_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7547815.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52607..53971
                     /locus_tag="RD444_000413"
     CDS             52607..53971
                     /locus_tag="RD444_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7547816.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54142..55212
                     /locus_tag="RD444_000414"
     CDS             54142..55212
                     /locus_tag="RD444_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547817.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55227..55895
                     /locus_tag="RD444_000415"
     CDS             55227..55895
                     /locus_tag="RD444_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547818.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55915..56493
                     /locus_tag="RD444_000416"
     CDS             55915..56493
                     /locus_tag="RD444_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7547819.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56896..57807
                     /locus_tag="RD444_000417"
     CDS             56896..57807
                     /locus_tag="RD444_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7547820.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58063..58980)
                     /locus_tag="RD444_000418"
     CDS             complement(58063..58980)
                     /locus_tag="RD444_000418"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDT7547821.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59182..59619
                     /gene="zapA"
                     /locus_tag="RD444_000419"
     CDS             59182..59619
                     /gene="zapA"
                     /locus_tag="RD444_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDT7547822.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59677..60225
                     /locus_tag="RD444_000420"
     CDS             59677..60225
                     /locus_tag="RD444_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDT7547823.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60340..62700
                     /locus_tag="RD444_000421"
     CDS             60340..62700
                     /locus_tag="RD444_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDT7547824.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62787..63101
                     /gene="trxA"
                     /locus_tag="RD444_000422"
     CDS             62787..63101
                     /gene="trxA"
                     /locus_tag="RD444_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDT7547825.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63283..65061
                     /gene="uvrC"
                     /locus_tag="RD444_000423"
     CDS             63283..65061
                     /gene="uvrC"
                     /locus_tag="RD444_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDT7547826.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65206..66105
                     /locus_tag="RD444_000424"
     CDS             65206..66105
                     /locus_tag="RD444_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547827.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66098..67360
                     /locus_tag="RD444_000425"
     CDS             66098..67360
                     /locus_tag="RD444_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547828.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67521..69419
                     /locus_tag="RD444_000426"
     CDS             67521..69419
                     /locus_tag="RD444_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDT7547829.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69718..70437)
                     /locus_tag="RD444_000427"
     CDS             complement(69718..70437)
                     /locus_tag="RD444_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDT7547830.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70774..71106
                     /locus_tag="RD444_000428"
     CDS             70774..71106
                     /locus_tag="RD444_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547831.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71093..73096
                     /locus_tag="RD444_000429"
     CDS             71093..73096
                     /locus_tag="RD444_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDT7547832.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73115..73585
                     /locus_tag="RD444_000430"
     CDS             73115..73585
                     /locus_tag="RD444_000430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDT7547833.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73606..74190
                     /locus_tag="RD444_000431"
     CDS             73606..74190
                     /locus_tag="RD444_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDT7547834.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74205..75191
                     /locus_tag="RD444_000432"
     CDS             74205..75191
                     /locus_tag="RD444_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDT7547835.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75181..75492
                     /locus_tag="RD444_000433"
     CDS             75181..75492
                     /locus_tag="RD444_000433"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDT7547836.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75514..77295
                     /locus_tag="RD444_000434"
     CDS             75514..77295
                     /locus_tag="RD444_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDT7547837.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77288..78664
                     /locus_tag="RD444_000435"
     CDS             77288..78664
                     /locus_tag="RD444_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDT7547838.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78671..79306
                     /locus_tag="RD444_000436"
     CDS             78671..79306
                     /locus_tag="RD444_000436"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDT7547839.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79336..80691
                     /locus_tag="RD444_000437"
     CDS             79336..80691
                     /locus_tag="RD444_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDT7547840.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80883..82277)
                     /locus_tag="RD444_000438"
     CDS             complement(80883..82277)
                     /locus_tag="RD444_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDT7547841.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82369..83340
                     /locus_tag="RD444_000439"
     CDS             82369..83340
                     /locus_tag="RD444_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDT7547842.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83337..84118
                     /locus_tag="RD444_000440"
                     /pseudo
     CDS             83337..84118
                     /locus_tag="RD444_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(84287..84658)
                     /locus_tag="RD444_000441"
     CDS             complement(84287..84658)
                     /locus_tag="RD444_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7547843.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            84792..85235
                     /locus_tag="RD444_000442"
     CDS             84792..85235
                     /locus_tag="RD444_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDT7547844.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85278..86087)
                     /gene="yidA"
                     /locus_tag="RD444_000443"
     CDS             complement(85278..86087)
                     /gene="yidA"
                     /locus_tag="RD444_000443"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDT7547845.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86106..87473)
                     /locus_tag="RD444_000444"
     CDS             complement(86106..87473)
                     /locus_tag="RD444_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7547846.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87457..88287)
                     /locus_tag="RD444_000445"
     CDS             complement(87457..88287)
                     /locus_tag="RD444_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDT7547847.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88450..88887
                     /locus_tag="RD444_000446"
     CDS             88450..88887
                     /locus_tag="RD444_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDT7547848.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88963..89583
                     /locus_tag="RD444_000447"
     CDS             88963..89583
                     /locus_tag="RD444_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDT7547849.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89772..90278
                     /locus_tag="RD444_000448"
     CDS             89772..90278
                     /locus_tag="RD444_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDT7547850.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90295..91194
                     /locus_tag="RD444_000449"
     CDS             90295..91194
                     /locus_tag="RD444_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDT7547851.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91281..92166
                     /locus_tag="RD444_000450"
                     /pseudo
     CDS             91281..92166
                     /locus_tag="RD444_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(92358..92429)
                     /locus_tag="RD444_000451"
     tRNA            complement(92358..92429)
                     /locus_tag="RD444_000451"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92394..92396),aa:Arg,seq:ccg)
     gene            92489..93823
                     /gene="rpoN"
                     /locus_tag="RD444_000452"
     CDS             92489..93823
                     /gene="rpoN"
                     /locus_tag="RD444_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDT7547852.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            93972..95063
                     /locus_tag="RD444_000453"
     CDS             93972..95063
                     /locus_tag="RD444_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDT7547853.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            95104..96105
                     /gene="gap"
                     /locus_tag="RD444_000454"
     CDS             95104..96105
                     /gene="gap"
                     /locus_tag="RD444_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDT7547854.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            96215..97405
                     /locus_tag="RD444_000455"
     CDS             96215..97405
                     /locus_tag="RD444_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDT7547855.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            97509..98264
                     /locus_tag="RD444_000456"
     CDS             97509..98264
                     /locus_tag="RD444_000456"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDT7547856.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            98380..99678
                     /gene="eno"
                     /locus_tag="RD444_000457"
     CDS             98380..99678
                     /gene="eno"
                     /locus_tag="RD444_000457"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDT7547857.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        33099 a        17048 c        20559 g        29063 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatctatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcca
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttt agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaattgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggct agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcatct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tttcacaata
    12961 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    13021 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    13081 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    13141 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    13201 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    13261 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    13321 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    13381 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    13441 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    13501 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    13561 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    13621 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    13681 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    13741 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    13801 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    13861 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    13921 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    13981 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    14041 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    14101 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    14161 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    14221 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    14281 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    14341 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    14401 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    14461 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    14521 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    14581 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    14641 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    14701 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    14761 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    14821 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    14881 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    14941 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    15001 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    15061 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    15121 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    15181 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    15241 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    15301 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    15361 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    15421 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    15481 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    15541 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    15601 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    15661 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    15721 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    15781 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    15841 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    15901 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    15961 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    16021 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    16081 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    16141 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    16201 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    16261 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    16321 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    16381 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    16441 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    16501 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    16561 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    16621 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    16681 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    16741 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    16801 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    16861 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    16921 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    16981 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    17041 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    17101 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    17161 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    17221 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    17281 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    17341 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    17401 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    17461 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    17521 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    17581 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaagaaa agcgtttagc
    17641 agaattgaca gcgctttttg atatgtatag tcatcaaaat gaaacaatcg caagtttttc
    17701 acatggaatg cgtcaaaaaa cgattttgat cggcacgttg ctgccagatc cagatatttg
    17761 ggtattggat gaacctttgc aaggacttga tcctcaagca gcatttgatt tgaaagaaat
    17821 gatgaaggct catgccgcaa aaggaaaaac agtcattttt tctacacatg tattggatac
    17881 cgcacaacag ttatgcgata aactggctat tttgaagaaa ggtgaattga tttatcaggg
    17941 ggcagttagt gaattattga atggctcacc tgatgaaacg ctagaaaaga tttatttgaa
    18001 aatggctgga aggcaagcga caggagatgg accgtatgta taagcgacaa ttattagaat
    18061 taattcgagt taatctttta tatgttaatc cacagctcac gaataaagca agagagaaag
    18121 gaaaaaaggg aaagcgatta actatctatc ttttgtctca aaatcttttt tcagggctga
    18181 tttttttact gatttacgga tttgctatgt ttgctatgaa ctttgttaaa ttgccaggtt
    18241 tctttactta ttacgtggca ctttttggta tgttaggttt aactcaaagt atatcagtca
    18301 tttataatat cttttttgaa ggaaatgacc tgcaagtatt tttgcctctg ccttttggac
    18361 aagaacaggt tttttcagca aagattttgg tagttgcttt gaccatcatt ccttttgttt
    18421 ttccgcttct agtcatgttt attttaaccg gatggcgagc tgatctaggg attttgctta
    18481 ctatcattat ctcaatcctt ttgttttctc tatttttgat tattcttttt tgtctggcta
    18541 gtttgattgt ttttggtttg gctcgaacag cagtgtttaa aaaacataga aaaacagttt
    18601 ctagtatctt gcttggcagc tcgatgctga tcgcatttgc tggaatcatg tggatgaatc
    18661 atcaaacggg gactattgaa aatgagttaa cagatcggca tccgatttcg atattattgc
    18721 cattctatga tgctgccagt cagccacttt ctcaacaagg tttattgggt tggggaatac
    18781 tagctatcac ggctctggga tttttccttg ctgtaaaaca gctgatagta ccaaagctta
    18841 cagagcaact gactgattca aacagtgagt tcaaaataaa aagaacccat aaaaagaacc
    18901 aaaatgtgca tcagctttta tttagttaca atgctcaatt agtaaaagaa cctaatttga
    18961 ttatgcaagt tctttcttct tccttgttaa ctccaatcat ttttattctt gcttttgccc
    19021 taggaggcga aataaaatta actgatttag ataataagtt aatcggtgtt gttttcctgg
    19081 tagggatcgc attggctttc ttaacagtta atcagacttc ttttatcagc aatcttattt
    19141 ctttggatca agaaaatttt ctgtttatcc gttctttgcc aatctctatg aaccaatatt
    19201 taaaagaaaa gttcagattc ggatggatat tgcagagttt tttaactggt gggatcgcat
    19261 tattaagcgg actgtctttt cagctgcctt tgttttttat ccctagcttg ctatttggtt
    19321 ctttattcgg ctgctactta ttgtcactac gttatttcgc tagagattat cggttgctgc
    19381 tgctgaactg gaccaatatt aaccaattat ttaacagagg ttcaggaagt ttgggtttag
    19441 tcgcaactat gatgggttca ttgatcatca gtattattct tatcgtctta tacggaatgt
    19501 tggctttgtt ttatccattt tggttagtga atattcctgt gatcggttta gttctaatac
    19561 tttccgctct atggatcgtt tattatcaac gaacgttttg gaaaaaattt gaataaagaa
    19621 gaggggccgt gacaaaaatc atgtcgcagc ccctttttta cagatagagg gtgttcaaaa
    19681 gtcagtttct cagtattaag tcaattttat gtaaaaatgt gagaagctat ttttacaaaa
    19741 ctttttttaa tactcataat tacttcttat tgctcctgcg gttacttgtc gcaatgtttg
    19801 tgagatattt tgtcatcgct caaggctgaa cgtctctttc acaacctttt ctttgttaca
    19861 cttcgtttat ttaaaaagcc gtttttttcg tttcatcatg attgcgtcac gctttacttt
    19921 gaaattacgg atgttatggt taaagacttc tgataataca agacctaaag cgattgctcc
    19981 agcaatcata attgcttgga ccgcataatg agccgcttct cgataggatt cagtaacgag
    20041 atttcgaacc gcttgataag ctaaaccacc agggactaga ggaaccatcc cgggaatatt
    20101 gaaaatcgtt acgggcattt tcaaacgttt agagaaaaga tcgctgatga atgctactcc
    20161 taaagaacca agcaatgaac caaaagcagc agatccgcct atttgaacac agatccagta
    20221 gatcatccag ccaaaagcac cagaaagacc acaacaaatc aatgaacgcc taggtacatt
    20281 tgtaataacg gcaaatgccg cagtcgctaa aaaactaaaa gaaaactgaa taaatatcgt
    20341 ccccatactt gtgttccttt cctaataaaa aagctggaaa ataaaagcaa tcgcaaaacc
    20401 gatcatcgta gcagtcatca tcgcttctgt tccgcgagaa actcctgaga cataatgtcc
    20461 agctagcaaa tcacggatag cgttcgtaat ttgaactcca ggaactaaag gcatcacaca
    20521 tccgataatg attagatcct gattgcttcc taagccaaaa cgtacgctgt agatagcggc
    20581 tatgccaatc aataaagaag ataaaaattc cgataaaaat ttgattctaa aaaatttcaa
    20641 actatataga taagctgcgt atcccaatcc tccaatgact aaagtcacag gtaaatcggt
    20701 catctttccg ccaaataaca ccataattgt tccactgatc actgccgcgc taacgatttg
    20761 caaccagaca ggaaagaaag aacggtctcg gtcgattgct tgcagttcgc agtaaagttc
    20821 ttctaatgtt agctctgaag caacatattt acgtgaaaga tcatttactt ttactacttt
    20881 ttctaaatta atcgaacggt tggtaatttg ttccattcgg atggtcgagg tacgatcaag
    20941 accaataaag agaccggttt gagtgacata gctgaccaaa cggaaatttc cagaagctaa
    21001 agcaattcga ctcatcgtat cttctacccg atacatctcc gcattgcttt ccatcatgat
    21061 tttcccagcg agtaaacaag tgtccagcaa cagatctaat tcttttcccg tcatagcatc
    21121 ttctccaata attgattgat tagttttatt ttactacaaa acataccaga agcgtaaaga
    21181 ataaaaaaac ttgaggaagg aataaaagag gcgtatactg tttttgtatt gcgggtgtag
    21241 tttagtggta aaatcccagc ttcccaagct gatgtcgcgg gttcgattcc cgtcacccgc
    21301 ttaaaatagg ttgtgaaaaa gctgctctgc aacaagtaat aagaacagcc aagcaaaaac
    21361 aacttcttat gtttttaact tggatggact tattaccgca gatgcaagct tttgcgcacc
    21421 gtttaccaag cggttgtgaa atcagtgtat tgtcttgagt atgaagaaaa tttttgaata
    21481 ataataaata ccttacagat agagtggtga ctgattagag acgaggaatc cgtctctttt
    21541 ttgtattttt aagccatctt aaaacaggtt ttattttttg ctgtttctat ttctttctag
    21601 tatttattta taggggaaca tatgttaaga tttagggagc tgttagtaaa ggagtaaaga
    21661 aatgagcaaa aaaacaacca aggcgatcgt ggggatcgtc gctgctgtga ttgcaggggc
    21721 attcggtgtt tccaccacac aaaaagattc agatatcttt cagcagatta caactatttt
    21781 tgattccaca ccacaagctt ctcaagtagc ggatgtgcca gaatatgatg gaaaacatca
    21841 agaaatagaa atcaatggga atcagccgga atttacaaaa gaggatctta gcctttccaa
    21901 aggaacatgg gaaagctatt caaatattga taaattgaac agagtaggac aagccaatgc
    21961 tatgcttgga aaagatttgt tccctactga aaagagagag cctttgtata ttgacccgac
    22021 aggttggaaa caaaagaaat taagtgacgg gcaatggtta tataatcgca gccatttaat
    22081 tggtttccag ctgactggtc aaaataacaa cattaaaaac ttgatgacag ggacacgttc
    22141 gttgaatgct ccatacatgc ttgctcacga aaatgatatt gcggcatata tcaaagaaac
    22201 aaatcatcat gtacgctacc gtgtcactcc tcattttgag gggaatgaac tggtagccag
    22261 gggagtgcaa ttggaagcag aaagcattga agacaaaaaa attgaattca atgtctttat
    22321 ctacaatgtt caagatggct acacgatcaa ttatgagaca ggacaagcaa caaaaacaaa
    22381 aaattagtag cagctattcg aattttttat gaatattatg aaaaaattgt gtaaaaacat
    22441 acattcaaag aaatatagcg tatactttat gaaaagagta cttttgataa agctacagaa
    22501 aaaaggaaaa aaacatacaa atagtgtttc ttaatttatt cggaagtttg gaatatttgt
    22561 ttgtagtttt ccctttcttc gagtaaaata aggttgtaga aggtaagctg gaattacctt
    22621 gcgggaaaga ggtgtagaat catgggtttt acagatgaaa ccgtacgttt tggttttgac
    22681 gatagtcgca aaaaagaagt gagcgaaaca ttagcgattg tttatcgggc tttggaagaa
    22741 aaaggctaca atccaatcaa tcagattgtc ggctacttgc tttctggaga cccggcctac
    22801 attcctcgtt atcgtgatgc tcgaaatttg attcgccgtc acgaacgcga tgagatcatg
    22861 gaagtaattg tcaaggacta cctctcaaat catggagtga atttatgaga gtaatgggct
    22921 tggatgtcgg ttctaagaca gtaggtgttg ctatcagtga cccctttggc tggacagctc
    22981 aaggagtaga gatcattcgg atcaatgaag cagaagaaga gtttggcttt gatcggttga
    23041 aggaattagt agcacaatat gaagtagacc ggtttgtagt tggattacct aaaaatatga
    23101 acaattccat cggaccaagg gcagaagctt ccatgtcata cggagaaaag atcaaagaac
    23161 tattctcctt gccagttgat tatcaagatg aacgattaac cactgttcag gcagaacgta
    23221 tgctagtaga gcaggcaaat acttcaagag ccaaacgaaa aaaagtcatc gataaattag
    23281 cagctgtgat gatcttacaa aattatttag acggctcagg taagcaatta ttttaataat
    23341 aaaagaaaag aggtaatctt atgacagaac ataaccattc ccacgaccat gaaggacacg
    23401 aacacatcac attggtggat gaccaaggaa atgaaacatt atatgaaatc ctactaacag
    23461 ttgacggaca agaagaattt ggacgtaact atgttctttt atatcctgct ggtgtttcag
    23521 aagatgaaga tgtagaatta caagcttatg catacatcga aaacgaagat ggaacagaag
    23581 gcgagttaga acaaatcgaa acagacgcag aatgggacat gattgaagaa gtattcaata
    23641 cattcatggc ggaagaagaa gaatagacta taaagctagg agaaaccttg atatgatagg
    23701 gtttctcctt tttttatttt ttactgtatg ccatattgta tgccatctag tcaatattta
    23761 agtgttgtac taatttttca acagtctttt ctttcgtttc ttttgccaag tgaccgtaac
    23821 aatcgatcgt ctgactaatt tttgtatgcc gtaatctagt ttgaacttct accatagtag
    23881 ctccagcgtc caagagcatt gaagcatgag tgtgtcttgc tacatgcaca ccgagaaatg
    23941 gaaccatagc tttttcacaa atagtaatca atcttgcata aatagcgtgg tgtgtgatta
    24001 gttctttata gataccacaa aaaatcatag tcggatcagt tacaccaagt gtcagcatat
    24061 atttccgctg attaatctgc cattttttta atatagaaat tgttctttca tctagtggaa
    24121 gaaccccagc actacaatca gttttaggat cactgatttt atagccattt ttggattggc
    24181 ttagtgtttt attgatggtg accgtatttt cttgaaaatc aatgtctgac caattcaatg
    24241 caagggcttc actgatccta accccgctat aaaagagaaa gcgtagtaac gttttatcaa
    24301 agtcgtttcg ataagtttca ggtttgctgt ttaggtaatc aaacaaggtg atcagttgtt
    24361 ctttactgta aactttgacc ttttctttat gcttagtggt ggttctttta ggcattaata
    24421 cgttgtctag tggattagtc tcaataatcc caagattgat agcgtagcta aaaatacgct
    24481 tagaagttga atgaagtacc ttgtattggt cagtagtttc agcccagcta tttacaattt
    24541 gttgggcttt ttttaccgtg acttcattca cacgaagatt tccatatttt ggaagaatcc
    24601 actttctaaa cttgatttca gttttcatga aggtggcttc tcggacagtg gttttatatg
    24661 tttctagcca caagtcataa acttctttaa acgtttcgtt gttcgctttt ttaataccgt
    24721 ttttatcgta gtccacttgc aagcgtgaca aggcaagctg ggcttctttt tttgttttgt
    24781 aacctcgtct acgaacctta tcaggtttgc cagtcatttt gttcattcca agataggctt
    24841 catagagcca aaatttacct tttttagttt catattgctt gaatgttgcc ataatagttt
    24901 tccctccata gtgtagcaag ccgtgggggc ggttggagga ggcagtttat tattattttg
    24961 aatagtgttt atttagctca gtaacaagag ctgtgaactc ttttagaaga tcattttcat
    25021 aaaattcttg atactctttt ttctcatctg tatttttttc tgaatcatta attaatgagt
    25081 catatcgtaa aagcgtattt agaagagctg cgagataagc aattggggta ctcccgtcag
    25141 gaaattcaat tcgagatcct gttttaagat attttattgc gtttgttaaa taaatcttct
    25201 gtgagacagt caggttatct tcttcgatgt tattgatagc attagaaaga ttagaatcaa
    25261 ttatagagtt atttttttca atagtttttt tcaattgctt ttcaagcaca tttcgcttaa
    25321 tttcgggcat catatgcata tctcttaatg tataagatcc gtttaataga taggtagtgg
    25381 atacatctaa tacctttgaa agttgtttaa gcctggtgtt attgggaaga ttatagccat
    25441 tttcccaatt actaacggca cctttactgg ccggtgggga taatttacct ccaagttgtt
    25501 ccattgagag tcccttttca attctagcca gtctaattct ttgtcctgtc tccactgctt
    25561 cggggtattt ttttctagcc acattttcac caccattcat atattttctc tactatatca
    25621 cgatttaaat atgtgtgaaa cataaaagtt caaaaaagta tgttaaaatt cttgcaaatg
    25681 aaaaggtatg atggtatatt gagtttataa gaggtataga aatgtattca agagcctctt
    25741 gcattttttt aaaatctttg tatagaaatg tatgctaacg aggggggata aatatggaat
    25801 taaaaattga caaactcacg cttgatgatc cagcttttaa aattattgac gatcgagttg
    25861 ttcaacaagt agagcaaact caaggaacgt tactttcatt attagaaaaa aagtaccagt
    25921 ttccaaaata tatgaacaag gcacaagctg ctaagtatat gaacgtttca tacaatacta
    25981 tgatgcagaa atatgttcct aacggtttaa agttgattat agttgatgga gtcgtaagaa
    26041 tttcacaaga tgaatgtgat cgctttatgg aagaatacca aaaataaacg caagccgtgg
    26101 gggcaaaggg attaatagga ggaaataata atggaatcga cacaaaataa agcaatcgaa
    26161 aaagtattat caacaattct tacagaagac acagcggcag aattagcaaa cttggaaggc
    26221 aaagcgctag aagaaacgtt tgaatgtttg tacgaacaaa tggactatca aaaattgcta
    26281 ccacaagaac caacggtaag cggagtattg cgtggcttga acgatttagt acaagcagag
    26341 tttaaagaac gactttctat tgaagagtat caagaaattt tgtatcagca agttgagttg
    26401 ttagcgagct tgctagggct tgaactggag gacgttgaat gaaaactgaa atttggaacg
    26461 gacacattat aagatttgtc gatattaatg atgaatggtg ggcagtggcg aaagatgttg
    26521 cggaagcgtt aggtcttaaa caggttacta gagccattca ctctttgcct aaagatgggg
    26581 ttactactag taaggtcatc gattcattgg gcagaacaca agatgtaaac atcatcaacg
    26641 aaaaaaaata tttaccgcat ggcattcaaa agccgtaaaa aagaagccga ggcatttcaa
    26701 gactggattt tcgacatcat caaagaacta cgccagtcca caggacttga aggctttcaa
    26761 gtattccgca tgctagacaa agaacaccaa aaagaagcaa tgactaaatt aagccacgct
    26821 attactgaac caaaaccagt agactacatt aaagcgaatg tgatagccaa taaagctgta
    26881 tcaactattt atggtcattc caaaatggta aaaaagaaag atatgactcc tgaaatgttg
    26941 gttgatcgtg aacctatttt agatgagaca gtagaattaa tgactgttaa agaaaaatat
    27001 gggttgcagt tttcagtgag tgagaaaatt tataatcgtt ctgctgaatt gcagacaact
    27061 taggagcatt aacatgatcg agaacaaaga aaaagcctta tccataacag tcttggcgga
    27121 caggataaag gcaacttaat tgggataaaa cccgatattc tttgttccga ttataccata
    27181 ttaaaaaatc ggagggaacc attatgaaac gaaacttaat tataggaagc attttaatcg
    27241 gcggaatttt cggcttatta ccattaattg caaaaatagc tatctttgca ggattagcgt
    27301 tatatctctt cactgcttat gacgagtacg actatcaatt aagaactgga gggcataagt
    27361 agatgtacaa aacaaaacta ttaaaccaac tcgatagcct tgaattagag gaaattaatc
    27421 aagggatcgc tgaactagaa aataatatcg ggaaaaccta ctttggaaat tcattcaatg
    27481 aaaaactaac agttttgtat gtactcaaaa aacatgcaga acacaaaata atttgtcgag
    27541 aaatcaacga gctaaaaaat cagattttaa ccgcatggtt aaacattact gatatgcaag
    27601 aagcaagggt gaaaacattt aatacatggg taaagtatca gaatcaatta aaaggtgctg
    27661 aatttgttcg ggatgggttg aaatacgaac ttgagcagtt gaagctcatg gaggtgtctg
    27721 aatgatttat accagtattt tgaaaaagga ggagaggttt attggcaaat aattatcaag
    27781 cagcaacgga attacaaaag tcgggatggt caatttatcc cttatctcct ggaactaaaa
    27841 ttccaataaa aggctctagt ggttggaatg atgcaactaa cgattctaat caaattaatg
    27901 aatggtggac ggaagatgca actagaaata ttgggttaat gcttgaagat catcaaatgg
    27961 tggtagttga cctagatcaa cattctgaaa cacgaaacgg cgttgagaat tataaaaaac
    28021 ttgcttcaat gtatgatcca tttccttcta cttatacgga aatcacacca ggtaaaggaa
    28081 tccatttctt ctttaaaatt cctgatggtg taattatcca acaagaaaca ggtgcatttg
    28141 ctgatatctt cgggcgtgat gataaaggta aaagtttaag cgggattgat attatcacta
    28201 aaggagttcc aatagcgcca actatgatta tttcaagaag tgttgggaga gcatatgaac
    28261 ctattgaaaa cgtatctcta aataatattg caccagctcc cgattggcta gtgaacctac
    28321 tgctaaaaaa accgcaagaa ataaagactc aataccgacc cgtagcaaaa acagggacag
    28381 caaaaaaatt agatatgatt gttcaaggcg ctggcgaagg tagtagaaac gatcatttaa
    28441 ctaagttatg cggttggtta ctatggcacg gtgtagataa tcaaacttta gcagaattga
    28501 tttatacagc aaatgcgtac aatgaaccac cgctaaaaga taaagaagtg aatcaaatta
    28561 ttcgatcgat gattaaaaaa gatttgaggg ggaaagctca tggctcataa gcataaatta
    28621 gatagtattt tagactttcc cgaagccagc gagcgtgaag acaatatcat tgaattgaaa
    28681 acctggatgt cacgattaag atgcaataaa gacgatcaga ttaaaagtaa cagtgttgtg
    28741 aatgcagaat tgattttaac caatgatagc aatttagctg gaacaattgc ttataacgaa
    28801 tttagcggat acattcacct attaaaagat tctccttgga tcaatcgtac cgctggagaa
    28861 tgggaagaca gttttgagga tgccttaacg gcatatattg aagaaaacta caatgtagtt
    28921 tttgatgaca acaaaataca caaagcagta gtaaatgtgg ctagaaagaa tgttttcaac
    28981 ccagtaaaag aacgtatcga aaaagttaaa tgggatcaac agcctagact ggaaacactg
    29041 tttattgacc ttttaggggt agacgacaac ctttacacac gagaagtaac aaaacgttgg
    29101 atagtcggat cagtcgcacg tatctataaa ccaggaataa agtttgagat cgttccagtt
    29161 ttggatggac cgcaagggat tggtaagagt accgttccag cattacttta cacggatgat
    29221 tttttcaccg attcattaga ttcgctaggt gaaaagaaag atgattatat gcagcttcaa
    29281 ggcaatgtaa ttattgaact tggagaatta tcttcaatga acaaaactaa aattgaacag
    29341 ataaaaaact tcatcagtgc caagatcgat aaaattagac ctccttatgg tcggaatgtc
    29401 atagcttggg caaggcaatg tgtatttatt ggaacgtcga atgatgggca gtatttgaaa
    29461 gatgatacag gaaaccgccg tttttatcct ttgccttgta agaataagcc aaagatgaat
    29521 ccgtttaaaa caaaggatga atattttctt caagtgttag ctgaagcaaa ggtgcttttt
    29581 gataaaggac aacgaatcta ttttagtccc gatgaagata aagaagtctt agaaattgca
    29641 aaagactatc aagaggatgc caagatggag aatccaatta aagaagcaat cactaaatat
    29701 ttggaaatgg aaatcccgta taaatgggaa gaagctccag cgtgggcgag acgttcttat
    29761 tatcagaact atcctgacag taaatgtaat gagaaaaatt tacagcattt ctataatgga
    29821 gctactatgc aacaagagtt ttatttagtt gatagcgtac tcaccgctga tattttggaa
    29881 gctgtttttg ataaacaagc aaaggattta ttgaatggtc gatcagatgc agaaactaaa
    29941 aagattgcct tgattattag cagtattcct ggttgggaac ggaagcaact gccaaaaaga
    30001 aataaacgac gaggatttta caataagtca aatgcctatg aaaataaaaa tcgagctgga
    30061 aagtacaaaa agtaacgggt agtaacgggt cagtaacacc tagcccgtta ctagtttacc
    30121 cttactgccc caagggatac agcaatttgg taacggggta acaccttatt tccttaaaac
    30181 ttttgaaata gttagctaat atgctgggga ataatgttac tggattttac ccgttacctg
    30241 ttaccaaaac gctagaaccc ttgtccccgt tgggattgag tggtaacggg tacctgttac
    30301 gtaggtgtta ccacgtgtta cgaatcaagg aggaaagtaa atgctcaata ttatttcaac
    30361 aaacaaagca ccgaactttc aatatacaga tgaaatggat cggtttctaa tgaacacgct
    30421 ggcatttagt gtgggattgg taacagaaga ttattccaca tttgatccag aagtattaaa
    30481 gataatggaa gaagaacctg attggctgca ggagtcagta gcgtggtgtc aatcgctagt
    30541 agtgggatca ttggtagata gtggcaacta tgatgataca ggtgagctaa tggatgaatt
    30601 taattgtctg cttaacctat atgatcgagc gagacaacga gagcttacat cgaatgagga
    30661 taatttgttt ttaaacatcc atgataagtt cttggctttg ctactaacag atgatgaatt
    30721 gataactaat ttattggagg tagaataatt atgcatatgg aaagtattag atacttcgat
    30781 ggtgagaaac acattgagta ttcaaacact caagaagatg tagactttat tagttttgag
    30841 gaagataaaa tcactaaagt taattttaaa gacggaacgt gcttgaagat agtttcgcct
    30901 tatattgaat acaaatcaaa atgggaacaa gatgattctg acgaacgaga tcgctttggt
    30961 tggtaatcta tggcagttaa aaaacaatgt aatcatgctg gatgtaaaac attgattgat
    31021 tataggcaga agtattgtgg gaagcataaa gcaaagcaaa cggcaattaa gcgtgaagaa
    31081 agaaagcaaa gcgaaggtaa atattttcaa ttctatcaaa gtagaacatg gcgaaaggct
    31141 tcatacttgt atcggttaaa tcatccagta tgtgaggatt gcctagaaga aggcttgata
    31201 aggaaagctg atgttgtaga tcacaaaatt gaactaaaag atgattggaa tagacggtta
    31261 gatgaaacta attttcgttc tttatgccat gcacaccata attccaagac agccaatgaa
    31321 agacaaagac gagaaaagag taccctttga gttaaggggg catagtgtga aacttactga
    31381 caatcgatgc ctactcattt tggtacaaat aaccgttgtg aaaaggcata agggtaatta
    31441 caaataacga ttacacttgt aatcacaagt gaaaaagtat ataatgaaag taggagattt
    31501 attgaaaaat acaattgtta ttactgataa aacagggaaa cagcgcacca ttgagttacc
    31561 tcattttggt caagtgacca ttcaaatgca gaacggaaaa atcatttata tagataaatt
    31621 agataaagaa aaattctgat cgaaaaacga aggaatgtga gcttaattgc ttacgttcct
    31681 ttttcttttg tctgaaagga ggacaacatg ggacacccta aaatattaca cgatacaaaa
    31741 ggcaatatat caagtgaaga aaaagcaatc cgtgtggacg ctagagaaga attgttcaag
    31801 cagcaaccat taataaatat cacgccccct gactggatgg cagcgagcgc tagaagtgaa
    31861 tggaatcgta tcgtaccaac attaaaaaaa gattatccat tgagtgaagc agattatggt
    31921 tcattggtag cctattgttt agcctttgct cgaatgaaaa cagccgaagc cgagataaga
    31981 aaatcaggta cgtttatcac atgcgaaaac ggagtaaaga aagccaatcc agcggttcgg
    32041 gttcaatcac aagctatgag tgatttgaaa aaacaagcca cctcactagg tatgacctta
    32101 gaatcacgat caaaactagc tttgaacaag gttaaaaatg atgaacccga agacccgttc
    32161 aaagagttga tgaattcatg aatgattaca ttgagaaagt cctatcaggt aagctgattg
    32221 cacccaagaa aattatccaa gcgtgtgagc gtcatataag cgatttggag cgttctaagt
    32281 cagatagctt cccttatgtg ttcgatgaag aacaagccac caaagcgatt aaattcattg
    32341 agttgctacc atctacggac ggtaaagcaa tcaagatgtt aggatttcaa aaattcattc
    32401 taggcagtct ttatggctgg cgtactaaag aagggaatta caggcgattc aatcgagcgt
    32461 ttaccagtat gagccgtaag aatgggaaaa cgtatatcgc aagtggcatg gctgccaatg
    32521 cgttgattat ggaacaagaa ccagcagaag caaggcaagt attgtttgta agtaacgctt
    32581 tgaaacaagc taaattgggc tatgatatgc tgtctaattc acttagaaac gtggtcaagt
    32641 ctagtaagtt tttaagacca caactgaaaa ttatgaactc taagattcag cacttgccct
    32701 ctaattcgtt cgctatggca ctggctagtg aaaccagcac gctagatggg tttgcaccaa
    32761 caaccgcaat tcttgacgag tggcacgaag caaaaactcg taaaacgtac aacgtcatta
    32821 agtcaggaat gacccaacaa aagaacggct tattgtgtgt tattagtacc gctgggcttg
    32881 atttaaacgt tcctatgtac gaggaatact tattgttaga tcgtgtgcta aaaggcgaag
    32941 aacaagccga caggtacttt atagcgatat gggaattgga cgatcccgaa gaaattcacg
    33001 atcaagagaa atggatcaaa gccaatccga tttttgaaag tgaagaaatc aaaaaagtaa
    33061 tgattccaac cattcaagat gatgtgaacc ttgctttgaa acaaaataac cttaattctg
    33121 tattggtgaa aaacttcaat ttatggagac aagcgagtga ggacagctat atgattgctg
    33181 aagactggca ggcaaccgaa gtagaaccac aagatattac aggcaagccc gtttatatcg
    33241 gagtggattt atctaaaaca gatgatttaa ctagcgtttc atggatcgta ccgctagata
    33301 acggcgaact ttattgtgat tcacatagct ttgtagccac caaatatggg cttcaagaca
    33361 aagaaaagcg cgacggtttg ccttatcgag aactagaaaa agctggtgag tgttccatta
    33421 ctcaattaga aagtggaatc gtggactatg accaagtatt tcagtttatt caagatttga
    33481 ttcaagaaaa tgatttggaa tgtatgggga tttgttatga cccgtataac gctaattcgc
    33541 ttatcagtaa agctgaaaaa gccaactacc caatgttaga agtaagacaa ggaacgatta
    33601 ctctaaacgt tccgacccga acttttagag aacaagttta tgaaggcaac gttattcaca
    33661 ataaaaatac gattctcacc catgcagtga acaacgctat tttaaaaacg gataacaacg
    33721 gcattcagat taataaatca aagaacagta acaaaattga tccaatagct gcattaatca
    33781 atgcctatgt gtttgcaatg gattacttca ccacaacgga aggagcgaaa gcagacaatg
    33841 aattttatac aagtgaagaa ttttctttct aattacattc ataccgttct tttacttctt
    33901 ggattggtgt gtgtgttggt tgctatcacc ttactaacaa atgtctacta tggcttgtta
    33961 gcgctgggca tagtgcttat tggggtagcg gtcatgctaa atacagaaca gaagggaggt
    34021 taaaagatgg cattttttaa agcgagacaa aatacaacgg gagatccttt cctacaacat
    34081 gtggtatcaa tccaatcgga tgattacacc accagcttta caagcgttcg tgcattaaga
    34141 aatagtgatg tgtttgcagc cgttcgaatt attgctagtg atattgcttc aagtccgatt
    34201 caattggtca aaaacaatat gccacaagct gataatgaac tggtgaagtt actaaacgag
    34261 aaacctaatt cagaaatgga tggttggcat ttaaagtttg ctttggcggt caatatgttg
    34321 ctaaacggta atagctttgc agagattaaa cgcaacggtg aaaaggtaga agaaattcac
    34381 ttattaccta actcaagtgt aacggttact caattagata atggtacgtt gtcttatcag
    34441 attggtgata aaaaaagacg tgtgaagtct agcgatattt tgcacttcaa atatttcacc
    34501 caagatggtt taacaggatt accaccactt tatgcgttac gtgatgaact gaaaattcaa
    34561 caagctggca accgcacatt gcacaatttc tttactcgtg gtgtcagtgg gtcaggtatt
    34621 ttgaaggttc ataagtcaga tttagacgga tcagcaaaga acgcaattcg tgaaaagttt
    34681 gaagaagcca acggttcaag tagtggagat aatgctctta gaacgatcat tcttgatgaa
    34741 acaatggact acaaaacatt agaaataaac accgatgttt tgaagcttat caattccaat
    34801 gattggaaca caaaacaaat tgccaaggcg tttggtgtac cgattgagcg tttaggcgtt
    34861 gaaaatgaac attctagcac cgttcaaagt aatctccagt atatccaaag tacgctgatt
    34921 cattacttta atgtgtttgt gagtgaatta gatacgaaac ttgaaaccaa tatccgcttt
    34981 aattccgatc agttactaga gactgatcct gaaacaaaag taaaaaatgt attggatcag
    35041 gtcaaagggt cacttctcac gattaatgag gggcggtcga aaatggggct accccccatg
    35101 gatggtggcg atcgtttact agcaagtttg aactttacgt atttagatac gttggagaaa
    35161 tatcaattaa aagaacagga aggagttaca ccagttgaat aatgaagaag aaaaggaaaa
    35221 acggctgaca gaagaagctg agctaacagc cgattctccc aaagtagaga aagaaaatga
    35281 aaaacaacca acagacggca aaactatttc aggctatgcg ttgaaattcg ggcaaccgtc
    35341 aaaagattta ggcggctttg tagaagtgat tacacccgaa gcattaaaag aggtggattt
    35401 atcaaatgtg ttcttattat atggacacga ttattcaaag ccactagcta gtgtcaaagc
    35461 aggtacgttg aaattgaatg tcgatgatac aggattacat tttgaagcag agctaaccga
    35521 cacgacctat tcaaatgatg tgtatgagaa tatctcaaaa ggggttgttg attctatgtc
    35581 ctttgggttc gtgcttggat tagattcatt cgacaaaaaa gaagatggca caattgaacg
    35641 atcaatagat aaaatcaaag cacttaatga aattagtgtc gtgaccgttc ccgcttatga
    35701 ttcatcaaat gtccaagtaa ataagcgttc ttacgaatcg tttatgagta acaaccaagc
    35761 aaaacaaaca aacaatagct tagaatccac ttctaaagca caaaaggaga gtaataacat
    35821 ggaaaaaact ttaatcgata acgaaaaaac agaattgcgt gggtatgaag aatatatccg
    35881 ttcacaaggc gaagtgcgtg atggagtcac tactgtaaat gcagcggcag ttgttcccga
    35941 agaagtaatc ggtgaagtct ttgatttgaa acgttcaaat tataacttag ctcaatatgc
    36001 aacagtaaaa acagtatcaa atggacaagg taaatatcca gtagcaacta accaacaagc
    36061 agtgttagca acaaaagctg aacttgctga aattggtgat attgacgctg aaatgtttac
    36121 ttcagttgat tataaagtag aaactcgtgc tggtaagatt gccttatcaa atgaggttgt
    36181 ggaagattca gcagtgaata ttgtacaaga ggtcaaagat cagttagcga aattggtaga
    36241 aaacactgac aataagcata tcatggattt attaaaaaca ttcactaaga aaacggctgc
    36301 tacgttggac gatttgaaac aactatacaa tgtggcatta gacccagcat taaataaaat
    36361 ggtaattcta aaccaaagcg gatattacca cctagataca ttgaaagatt cagatggacg
    36421 ttacatttta caacccgatg tgacagcacc tagtggtaaa tcattattcg gtatgccagt
    36481 agtattgatt gcagatacat tgtttgccaa tcctaaagct ggaacattcc ctatgattat
    36541 gggggatatt gcacaatcta tctttgttgc tcgtagaaat caagtaacga ctcaatggga
    36601 aaaattcgat tactactcac aaggacttgc agtgatcgtt cgcaacgatt ataagaaaat
    36661 tgatcaaaat gcttcagtgt atattgagtt tacgccagtt gtaacaccaa aagtatagaa
    36721 aaatattggg cggcggttta tcccactgcc tttttttcat taggagagat aaaatggtaa
    36781 ttttggatag cataaaaaaa agtatgcgga tcgatcacac tattgatgat gactttattc
    36841 aacaattgat tgatacagca ggcgaatata taaaaagtgc tattgatagt agcgcaacgg
    36901 ataaagatat ggataattat cagcaatttg atttggcggt gtcattactt actcaacatt
    36961 ggtatttgaa ccgtcaagaa gccagcagcg aacggatacc agtaacggta caagcattag
    37021 tacaacaaat gagaggtgct tattatgcca atcattaaga atgtaaatga attgactgag
    37081 agagtcaagt ttaaaaagac aaaacgggta aaagatgaag atgggcagat ggtggatagt
    37141 gaagaaactg tatttgaatg ttgggctaat gtgcgttcac aaatgctaaa agacgtgctt
    37201 gctagtgtag gtaccattct tgaaggaacg ttgacgttta ttattcgata cgatcaagat
    37261 tatgaactaa caaacgatat gaaagtagct tggaaaaaca agagttataa aattatttca
    37321 atcaatgaag gaacggcgtt taaagattat acaacgatca tagctaaaca gatttcttag
    37381 aatgattaca cttgtaaacg ttgtgggtat ttagtataat taaggtagta aatgaagggt
    37441 tagctgcctg aattttgcaa gacttagcta gtcgaaatct attgttggac tgaaaattgt
    37501 aggtgtggtt gcaaacatgc tggactaaga aagatgatag atgatgaacg ttcaatttta
    37561 atacaggcat gctaggactg attggatggt tttggcaagc tgattgtgta gaaatcaaag
    37621 aacacgtctg ttgtggacgt gttttttttg ttataattca aaagaggtga tttaatggac
    37681 aaagcagagt ttacaaattt ttattggaag tattatctac atttagaaaa tgagtttatt
    37741 aataccacag attttgtgat gctcgatgag aataatttca aaacattctc tattgaatat
    37801 caaaaactac tactagcgat tggttcggag tgtgagatta ttttcaaaga actttgcgga
    37861 tttgatagca gtagcaataa aagcataact gattttaaag ctgttattaa atctagcgaa
    37921 ttaatgtgtt tggataatgg tgttagggtt atgaattctt taacattgac ttcactaaaa
    37981 ccttttggag atgggtggcc tgaagataca ccagattggt ggaaaatata taacagagta
    38041 aaacatggtc gttcttcaaa ttataaggaa gcaaatttgg aaaatgtgtt gtatgcttta
    38101 gcaagtctat atttattaga ggaatatttg cataagaaag taatactaga aggtgaagta
    38161 gactttataa gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg
    38221 atgcaaaaag tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt
    38281 aaagaacaag aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt
    38341 gtatgccatt tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt
    38401 cataaacgct gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg
    38461 aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa
    38521 acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat
    38581 tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag
    38641 gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt
    38701 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact
    38761 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
    38821 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
    38881 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
    38941 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
    39001 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
    39061 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
    39121 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
    39181 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
    39241 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
    39301 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
    39361 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
    39421 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta tactatgata
    39901 aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac gggaaatgcg
    39961 tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt atgatatacc
    40021 attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga aaaatttttt
    40081 cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta
    40141 tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt ctaaatatgc
    40201 tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagaag cgaaaaaacg
    40261 aggaataacg acaataaggc ttaattctgc agaatacaga gtagaagcac accaatttta
    40321 tgaacacatc ggttatcatt ccgataaaat gcaaaaaaga tttttgaaaa tcctgtaaat
    40381 ctttatcaaa aaaatacccg atcaccaagc cgatacttga tgatcaggta tttttgtttt
    40441 tttgactaag aaagtaattg catcaagaaa ccagagatga tcaaaacgat ggcaccacca
    40501 agtcgattgc ccatttgagc aaaagcaata agttccatcc gattagctgc agataagacg
    40561 gctacatttc ctgttccgcc catcgagttg ttgcataagc cagcagtaat agttgattca
    40621 attggatata aaccaaacaa acgaccgaca aatcctgaaa caactgaaat cgcgataaca
    40681 cttgtcagac ataagacaac aaattgccat gtcaaagcag aagctaatac attcagattc
    40741 agcaacgcaa taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagtta
    40801 ttgaacatga tagctgcatc ttcataatac tctggtaata ggttggcgat tttacagatg
    40861 acaacgataa taatcataaa tgcgtaagca tggactttag gaacaaaata gttaccaatt
    40921 tgtcctaaga tgaagaaagt caatgatacc atcaaaccaa cacctaactg tggaatggaa
    40981 ggtttcacag ttcgaggttt tccatcgcca agatcgcctt tttccattaa tttaccattg
    41041 ccgttgaatt ttggcattga ttgtccagca cgagcaacta atgctgcacc gatgatagct
    41101 aagacatttg tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcatccgta
    41161 cctaaaacat ttgcataaat cgttgataaa ggaacggctc cggctccaac accaccagcc
    41221 atcgcaggta atgagatgta taaaacggaa tttgcaaaac catttccgat aagcatgccc
    41281 acgagtccta cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga
    41341 acagaagctt tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca
    41401 gcaatataaa agtcccaaaa gcccatattg ctgacaaagt tttctgtgga tttcactaca
    41461 tattcaggta agatcccaaa tgtagcaatc gcagcagaag cgaagatcgc aaaaacggaa
    41521 ccaccaccca aataactttt gacgataggg atttttgtcc cgataaaatg aaataaatta
    41581 ccgaaaataa ccaaaactgc gattggtcca atcatattcg tcggtaattt ccctaaagcc
    41641 attgcaatga tcaggacaag gaccatgatc aaataaagtg gcaaacttac gccggtgatt
    41701 tttgtggacc aaaaaccttc ttttttcttt tcctcaactg cagttgcagt tgttgttttt
    41761 tgtgccattt ttacagatcc tttcttattc tgtatcatat gaaaaaagcc aaagaaaagc
    41821 gacaaaagag caggagttgg ctggccgctg ttttgccgca gtcgcttttt tctttttaga
    41881 cgtcatacaa agtttctgta tgttctttta ctataacgat cttaatcttc taaagattta
    41941 tattcaggaa cccatttagc agattcaact gcttctttga tatccgtgat ttcttcacga
    42001 ttcaagtttt gtgcgacaac tgattttgcg acagtttcag caatcgtttg agaaaattca
    42061 gtgatttttg ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg
    42121 cctaatgctc gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca
    42181 atcaaaccaa gtcctaaacc tgggtacatc aaagcattat ttgcttgacc gatttggtaa
    42241 gttacaccat tatattcaac atcagcagca gggatacctg ttccaactaa tgcttttccg
    42301 tccgtccatt ctattaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa
    42361 ggcatgatga tcggacgtgg tgtatgagca gccatttctt tcacgatttc ttcagtgaat
    42421 gctccaggtt gggtagaagt accaatcatg attgtcggat ggatcgcttt tacaactgct
    42481 tctaagtttg tcaattcatc agcattagtg aaatctgagc gtttgcgagc gaatgggatt
    42541 tgaccaggag tcaatccttc tgtatcttca aacaataaac cttgtttatc tacttgatag
    42601 aaatgttggc gtgcttcttc ttcaggtaca ccttgacgga tcatctcatc taaaaggatg
    42661 tttgcgatac ccacaccagc agtaccagca ccaaatgtca aaattatttg atctttcaat
    42721 tgttcaccgg atatattcaa tccgcctaat acacctgcta ggacaacaat tcctgtacct
    42781 tggatatcgt cgttgaaagt agtgatttga tcttcgtatt tttctaagat cgctgcagca
    42841 tttcctcgtc caaaatcttc ccaatgcaat aacaattctg ggaataattc ggtagctgat
    42901 tgaacgaatt gatcgatgaa ttcatagtaa gtttcgcctt ctacacgagc atgacgattg
    42961 cctaagtata agggattatt caataattct tcattattag taccagcgtc gatcgataca
    43021 ggcaaaactt gtgctggatt aataccagca gcagcagtat agaccatcaa tttgccgata
    43081 gcgatgtcaa caccgttcac accccagtcg cccataccta agattccttc tgcatcagtc
    43141 acaacaatca aacgaatgtc tcgtccatct gccgcgttct tcaaacttgc tttgatgttt
    43201 tctggttcgt cgattgaaag aaatgcagca tcttgcggtt tcaagaaaat ttcgttgtat
    43261 tgttcgattg aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt
    43321 tcgcccatca atttataaaa caatgtccga ttcgtattga atagattcat taagaagata
    43381 cgtttttcca aatctgtttg tttgcttaag tattgaccat aagcttgaac agattgttgt
    43441 tccagtgttt ggacagtact tggaagcata ccagtgattc catattttgc ccgttcttct
    43501 tttgtaaaag ctgttccttt atttagaaaa ggattattta ataagtttaa ccctgtaagc
    43561 atgaatgatt cctcctaagt tttaatacaa acgaagtata gaagtcagat gatgatatag
    43621 tcaaaaagag caggtatgat aaattttatt tatatgtaat atagccaaaa gaaatcaaaa
    43681 tagcagtatt tgttcttttt atatcgacaa tccttgcttg aatactatgc gtatctctta
    43741 tatgctagag aaagaaaagt agaagaataa gggatagatg gaatggataa ataggatttc
    43801 tgaggaaaaa agcacatgat agcgttgata ggagtcacct atgtcaggtg ataaaaatac
    43861 ataagtagaa aattaagagg aggaagagaa aatgaaagaa gttcaattga ttagaaaaag
    43921 cgaactatca gaaggcggtt gcaatgcatg tggcgtagta gaagcgacaa gctatacatt
    43981 aaaattagat tccaatcaag caataatctc tgaactgaca gtaggcggtc ttgtggacag
    44041 tctggcgctt gctgaaggtt ttactggaga agatatttat gagatgtttt cagaagtccg
    44101 tcagttaaaa aaaggagaaa aatgcataga ggttcatcat gagagcccga atgtgaggtt
    44161 caaacgtggt gataatgaga tgatcttcaa gaatcatgtg tctgatcaca ctgaactata
    44221 tgaaatcgtg aatcaaatac tgtctgggat ttttgaatta ggaccttatg agttcaagga
    44281 agaaaacggt aatccaaaat taaatgaaga atggcaagaa acgattgaaa cacaaagaaa
    44341 caatccacac ttatttcaat agtcttccac agtatttcgt aaaaatgttg atgaaaaaga
    44401 aacaccgtga atcagaggtc gtgaaaaacc tgccctgcat caagtaataa gaacagccaa
    44461 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagtttt
    44521 tgaacaccgt ttaaggaaga gactgtgaag tcaacgttct gacctgagta ttagagagac
    44581 aaatggcaaa atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg
    44641 attgaacacc gttttatcga gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    44701 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac cgagaaaatg
    44761 gcttttgaac accgtttatt caaaaaaagg gactttgaca ctacttttgt caaagtccca
    44821 tcggtttttt atctattttt attacaatca tttcttccag agtagtgaca tctggaatgg
    44881 ctgaaatgta aacagattat ttgcctgtat caatgtcgaa gagtaatata aaaagtgtaa
    44941 acgctatcat attgttcatc attcaaattt tatagagaga aggcattgag atggaaaaaa
    45001 catatcaagc aggtacaaat gaaggaatag tagattttat caatatggaa gatttagaaa
    45061 tcgcggcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
    45121 acttatttac ttatcaagaa aatgaacggg cattcaatca tcgtttgatt attcctcatg
    45181 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacgaagaa atgctgactg
    45241 caccaatcat catggcacca gttgctgctc atggattagc tcatgtgaag gcggagaaag
    45301 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
    45361 gtactttaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
    45421 atatgagcaa agataatgga atcaatttgg atattcttga agtggccaag cgtaatggag
    45481 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
    45541 gaaacgggtt taccttcccg ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
    45601 gccaaacgat ggatgctgta tataaatctt ccaaacaaaa acttagtcca aaagatgtag
    45661 aatttatcgc tgctcattct gatttgccag tctacgtgaa aggtgttcaa tcagaagaag
    45721 atgtctatcg atcattagaa tctggtgctg gtggaatttg ggtatctaac catggtggcc
    45781 gtcaattaga tggggggcct gctgcgtttg attcgttgca atatgtggca gaagcagtag
    45841 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg tggacaacat gttttcaaag
    45901 caatcgcatc aggtgccgat cttgttgcta ttggcagacc cgttatttat ggattgtctt
    45961 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
    46021 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
    46081 atcgttttat ttaagaaata aatgacgaaa aaaataggag tgcggcagat gatgccgtac
    46141 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
    46201 tgaaatattg tttcatactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    46261 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    46321 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    46381 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    46441 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    46501 aataccgttg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    46561 gaaaatccag caactggcca gacaggttct ttaacattgg atgtagttcc tgaattgcca
    46621 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    46681 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    46741 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    46801 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    46861 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    46921 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    46981 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    47041 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    47101 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    47161 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    47221 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    47281 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    47341 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    47401 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    47461 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    47521 cacatgcaga aacaactata cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    47581 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    47641 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    47701 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    47761 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    47821 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgaatttt
    47881 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    47941 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    48001 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    48061 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    48121 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    48181 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    48241 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    48301 tatgatctaa gtccagttcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    48361 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    48421 gaaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    48481 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    48541 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    48601 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    48661 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    48721 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    48781 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    48841 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    48901 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    48961 tgccggtgcc cccatcaagg ttactagcgg tagtttatta tcagcaggaa gccatacctt
    49021 gcgttttggg atggggtcgt gggatacaaa tactctattg gcagatatta ttgctattat
    49081 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    49141 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    49201 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    49261 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    49321 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    49381 agagataaat acgtggaata gttacatctc aacaagtgat tcaacaggaa aatataatac
    49441 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    49501 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    49561 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    49621 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    49681 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    49741 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    49801 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    49861 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    49921 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    49981 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    50041 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    50101 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    50161 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    50221 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    50281 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    50341 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    50401 cattcatctt acttacaact atcaattaaa tactgtagat acttctgtac aatccgtcag
    50461 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    50521 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    50581 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    50641 aagttattat gaagcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    50701 cacgtctcct tggcaactga cagggacatt gacttcttta tttaagaatg atcaaggtca
    50761 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaaacaat tgattcagca
    50821 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    50881 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    50941 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    51001 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    51061 aacttctgct gttaaaatca gaaatgcttg gaggagtgga cttggaactt ttcagtttgc
    51121 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    51181 agatttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    51241 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    51301 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    51361 gctacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    51421 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    51481 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    51541 ccggtccaga aagtcaaatt tgttattttt atttccaatt attttggttt ggacgtcctt
    51601 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    51661 aaaaagagtt tagttttact attacagaag aaggacgatt gaaatttgaa atatggcttt
    51721 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    51781 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    51841 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    51901 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    51961 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    52021 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    52081 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    52141 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    52201 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    52261 ttgaattatt tgctcgctat gcatctattt tgcggacgct gatcaagcag ttagaattac
    52321 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    52381 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    52441 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    52501 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    52561 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    52621 cattggtgct tcccatgcag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    52681 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    52741 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    52801 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    52861 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    52921 taaattaatc gtagcaacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    52981 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    53041 agcaaaagaa gtagtgatct tcggtgctgg ttatattgga ttggaattag cagatgcttt
    53101 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    53161 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaatcaattt
    53221 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    53281 acttctatct caagcaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    53341 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    53401 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    53461 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    53521 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    53581 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    53641 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    53701 tgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    53761 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    53821 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    53881 acacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    53941 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    54001 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    54061 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    54121 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    54181 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    54241 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    54301 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    54361 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccagacacc tggagtcgtg
    54421 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    54481 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    54541 gtaggcgata ttagtttgaa agaagaatat ggtctagccg taggagatac agttaagtta
    54601 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    54661 tcaccagttc tctatacaac catcaatgct taccaagaga tccgcttcga aaaagaagat
    54721 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    54781 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    54841 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    54901 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    54961 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    55021 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    55081 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgctggta
    55141 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    55201 gcaattggat aagaaggagg aagagcatga aagcaataga atttaaagaa gtagaaaaaa
    55261 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    55321 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    55381 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    55441 gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    55501 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    55561 tcaaacatga acatcgtcaa aaagaagcaa aagaattatt tgagcagctt ggcgtggata
    55621 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    55681 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    55741 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    55801 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    55861 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    55921 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    55981 tccaagggat atctcggcac ttcaacaaga gagatcgcac aaaaagttgg gatcacccaa
    56041 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    56101 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    56161 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    56221 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    56281 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    56341 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    56401 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    56461 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaaatagtt
    56521 aggtgaagaa gcaagatatg acgaagatcg ttatatcttg ttttttttat ataggtagtt
    56581 tttcataaat acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat
    56641 gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga
    56701 aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata
    56761 tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta
    56821 cagccccttt tttgcgtact taagctgatt ttaagttttg acccttagtc tggatctacg
    56881 aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt
    56941 attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc
    57001 agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca
    57061 ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca
    57121 cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat
    57181 cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc
    57241 acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct
    57301 gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat
    57361 ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt
    57421 tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa
    57481 gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa
    57541 gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt
    57601 gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa
    57661 gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat
    57721 agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca
    57781 caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc
    57841 atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca ccgtttaagg
    57901 aagaggttgt gagcctgccg tttaactccg cgtcacaagg agcaatttca aaaaacagct
    57961 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta
    58021 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagcagctt
    58081 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    58141 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgatggaag
    58201 ggtgattcca gcttcagctg atgctttgtg taactcttcc aaaaagctgg cacgactgat
    58261 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    58321 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    58381 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    58441 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    58501 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    58561 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    58621 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac caaaatagct
    58681 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    58741 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    58801 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    58861 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    58921 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    58981 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    59041 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    59101 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    59161 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc
    59221 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    59281 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    59341 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc
    59401 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    59461 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    59521 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    59581 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    59641 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    59701 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    59761 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    59821 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    59881 gatcaggtat ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    59941 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    60001 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    60061 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc
    60121 caaaatcaat tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt
    60181 accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg
    60241 aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg
    60301 gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa
    60361 acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga
    60421 aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt
    60481 caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa
    60541 cttgaaaata tccgtcccca tatgaaacgg atcgagatcg gagcagactt gaatggagtg
    60601 gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat
    60661 gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc
    60721 acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact
    60781 gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct
    60841 gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg
    60901 atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta
    60961 tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat tgaaccaaaa
    61021 caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt
    61081 caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac
    61141 aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa
    61201 gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga
    61261 catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat
    61321 caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta
    61381 ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa
    61441 atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt
    61501 ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa
    61561 aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct
    61621 ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg
    61681 cattatcctg aattgaaagt ttatggatac aatcgggcaa atacgattaa tgccagcatg
    61741 gagttcgatg tggatacact aagtccaacg tatcgtttgc tgattggcgt tcctggacga
    61801 agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg
    61861 aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat
    61921 cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa
    61981 gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa
    62041 atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga aaaagcagaa
    62101 aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag
    62161 catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag
    62221 aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg
    62281 ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa
    62341 gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct
    62401 caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg
    62461 ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa
    62521 tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga
    62581 acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc
    62641 tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa
    62701 agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta tactaatcat
    62761 agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg
    62821 aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc
    62881 gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa
    62941 ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca
    63001 ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt
    63061 caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa
    63121 gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta
    63181 ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga
    63241 catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa
    63301 aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga
    63361 acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact
    63421 ggcagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc
    63481 gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat
    63541 cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat
    63601 gaaaaatatc cccgtttggt gattactcgt aaagtactaa aagacaaagc actgtatttt
    63661 ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt
    63721 cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga
    63781 caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat
    63841 gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa
    63901 atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc
    63961 cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga
    64021 gatgtatttg gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt tttcgtaaga
    64081 caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa
    64141 gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa
    64201 gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg
    64261 ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa gaatgcctca
    64321 gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca
    64381 gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat
    64441 aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa
    64501 ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat
    64561 gcatcaatga gagaagtcat ctatcgcaga tattcacgag tgattcgcga agggttgcct
    64621 ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc
    64681 ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa cgataaacat
    64741 aaaacaaatg agttgctatt cggtatagat ttggctgtag taccgcttga gcgaaattca
    64801 ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc
    64861 catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta
    64921 ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca
    64981 gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt
    65041 gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca
    65101 ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc
    65161 tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag
    65221 gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc
    65281 gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt
    65341 ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt
    65401 ggggagaaag attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa
    65461 gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag
    65521 attgaaccaa aaatcgattt ttggatggaa aaatttcagg taaaagggaa aaagacggat
    65581 aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc
    65641 catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct
    65701 gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct
    65761 agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga
    65821 aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta
    65881 ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg
    65941 atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa
    66001 atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact
    66061 ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac
    66121 caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc
    66181 ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt
    66241 caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa
    66301 aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc
    66361 ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat
    66421 gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac
    66481 tggcttgcaa tcgatgcttc gagcaagtca attaggcatt tcaccagaag aagtagctag
    66541 cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat
    66601 gacagttgga gaagataact cgacagtcca atatgtcgta agttatgtag caacgattgt
    66661 cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa
    66721 aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg
    66781 aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt
    66841 tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat
    66901 ctcattggat aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg
    66961 cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga
    67021 tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggatata tgcttggatt
    67081 gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt
    67141 cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta tgggtggagc
    67201 attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa
    67261 catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt cattgaaaca
    67321 atcgatttca attatgagaa atgaacgaaa aaaagcataa aatatagaaa aatatagaag
    67381 gaagctggac atttattgtt cccagcttct tttttgtata gacctatttc tattttttta
    67441 ttatattgga tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac
    67501 caatttttgg aggcgtttta atggaccgct atttagattt actttcagag aagttcccta
    67561 caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    67621 gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc
    67681 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg
    67741 aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg
    67801 attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt
    67861 ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct
    67921 atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa
    67981 aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg
    68041 cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc
    68101 gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc
    68161 agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat
    68221 tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg
    68281 gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat
    68341 ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc
    68401 atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg
    68461 aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg
    68521 ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg
    68581 atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc
    68641 agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc
    68701 tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc
    68761 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa
    68821 aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga
    68881 tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt
    68941 ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc
    69001 acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag
    69061 aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga
    69121 tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa
    69181 caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac
    69241 attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa
    69301 aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg
    69361 aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag
    69421 aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg
    69481 cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta
    69541 tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc
    69601 ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa
    69661 attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta
    69721 aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag
    69781 ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc
    69841 aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc
    69901 ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc
    69961 cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt
    70021 tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt
    70081 caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa
    70141 gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg
    70201 gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat
    70261 tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc
    70321 ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg
    70381 gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt
    70441 tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga
    70501 cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg
    70561 aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa
    70621 taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt
    70681 attgaagggg cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag
    70741 aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa
    70801 caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg
    70861 aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa
    70921 cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa
    70981 aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa
    71041 aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt
    71101 cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca
    71161 agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa
    71221 ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag
    71281 cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg
    71341 cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga
    71401 agcaaattat tccgaagaaa atttcatgaa taaattagaa gaggtccttg atttaaaaga
    71461 aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct
    71521 tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga
    71581 gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga
    71641 tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc
    71701 tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga
    71761 aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc
    71821 cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat
    71881 ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa
    71941 agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga
    72001 aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag
    72061 tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca
    72121 tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga
    72181 agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc
    72241 agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt
    72301 tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat
    72361 catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg
    72421 tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt
    72481 atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa
    72541 tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct
    72601 gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta
    72661 tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca
    72721 atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg
    72781 ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag
    72841 tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt
    72901 agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc
    72961 ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg
    73021 cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa
    73081 aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa
    73141 tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg
    73201 ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc
    73261 cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg
    73321 attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg
    73381 attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc
    73441 ccaagggaaa gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac
    73501 aaaaggaatc atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc
    73561 cttcctatta gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa
    73621 aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa
    73681 gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat
    73741 caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat
    73801 cgccaacaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga
    73861 ttgtttgcag atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt
    73921 atcaaaaatt cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa
    73981 aaatcagcag cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt
    74041 atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa
    74101 ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt
    74161 tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac
    74221 taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga
    74281 tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc
    74341 catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc
    74401 gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga
    74461 tgcgctatac tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc
    74521 tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc
    74581 atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg
    74641 attattttga agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt
    74701 tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca
    74761 ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa
    74821 catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt
    74881 tgaactttgt agataaagat atggaagatt acacgaattt tctattagag acagattatg
    74941 ctgattttct gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt
    75001 tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac
    75061 tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg
    75121 ttatcggcaa aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct
    75181 atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt
    75241 ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg
    75301 gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa
    75361 acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa
    75421 ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa
    75481 aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc
    75541 tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta
    75601 gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt
    75661 caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa
    75721 gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg
    75781 ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg
    75841 cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg
    75901 gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc
    75961 atggtgccaa atgggataaa aggtacgatc aaaacaatcg aaaaaggttc attcactatt
    76021 gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa
    76081 aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg
    76141 atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct
    76201 gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc
    76261 gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt
    76321 ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg
    76381 gtattgattg ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg
    76441 ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac
    76501 tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct
    76561 ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct
    76621 ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct
    76681 gtatcgccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta
    76741 aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat
    76801 tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt
    76861 caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag
    76921 ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca
    76981 ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc
    77041 gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt
    77101 gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc
    77161 gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa
    77221 ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc
    77281 atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt
    77341 tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga
    77401 gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga
    77461 aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga
    77521 actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga
    77581 taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc
    77641 agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt
    77701 gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa
    77761 agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc
    77821 cgtagtcttt gctgctattg gaatcacttt tgaagaagca gaatttttca tggaagattt
    77881 tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc
    77941 aatcgagcgt atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga
    78001 aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg
    78061 agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta
    78121 cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt
    78181 tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt
    78241 gactggttat attacagaag gacagatcat cttgactaga gagctttaca agagtgggat
    78301 tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc
    78361 cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca
    78421 aggaaaacag gcaaaagaat tggccgtagt tttaggtgaa tctgctttat ctgatgtaga
    78481 caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta
    78541 cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc
    78601 aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa
    78661 gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa
    78721 aaaacagttg acaactgcca caagagggca taaattatta aaagataagc aagatgaact
    78781 gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga
    78841 gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggagccttt
    78901 catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa
    78961 tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac
    79021 acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt
    79081 tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat
    79141 ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc
    79201 tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga
    79261 agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatccttttt
    79321 taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgctt gccggctgtt
    79381 cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta
    79441 ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact
    79501 tctgctgtct gtgtgacggg attgacaaca ttgaatacag cagagcattg gaacagtgct
    79561 ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg
    79621 attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa
    79681 gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctgaaa
    79741 ttggcctttg tgatccaact gattggtacg gttgctttat cttttgtatt cgtgcctgaa
    79801 tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat
    79861 gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc
    79921 atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta
    79981 ctggaatatc atcagaaaaa gaagataacg attcattcaa aaatcgcatt aaccgttaca
    80041 ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggaaacg
    80101 ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct
    80161 agaacggctg gatattattc agttgattac ctccatatga gccatgcagg tctgatttta
    80221 acgatgtttt tgatgtacat cggtggaaca tctgggtcga cggccggcgg attgaaaaca
    80281 actacgttag gtgtgttgct gatccagatg cgttcgatgc ttaaaggacg tacgagagcg
    80341 gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt
    80401 gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct
    80461 aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt
    80521 cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt
    80581 atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa
    80641 agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt
    80701 ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg
    80761 gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctatccgttg ttgaacctcg
    80821 ggtagtgcct ctttcttatt gaaaggaatc aataagattt gattccttta tttatcaata
    80881 ggttataatt catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt
    80941 tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta
    81001 aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta
    81061 ggatcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt
    81121 tctactttga aaagagaatc aaaaagctga ttggctcttt tcgtaagact taatttagtt
    81181 tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat
    81241 ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgttttg gacatgtact
    81301 ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag
    81361 tagattgttt ggttaggtaa taatttttgg aaaggaggaa tgatcgtatg ttgatagtaa
    81421 gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact
    81481 tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa
    81541 ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt
    81601 cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat
    81661 tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt
    81721 gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag agattctaag
    81781 gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg
    81841 cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga
    81901 ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg
    81961 atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata
    82021 aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc
    82081 ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac
    82141 tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat
    82201 cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca
    82261 taagaattaa taggcatttt ctacactcct taaaattgta taccatacaa ttttaccatt
    82321 gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt
    82381 aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa
    82441 tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta
    82501 cctgacggaa aagacaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga
    82561 tattttggaa caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct
    82621 catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac
    82681 gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta
    82741 gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat
    82801 ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa
    82861 gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata
    82921 aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct
    82981 agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg
    83041 ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc
    83101 tatgtagatg attactcagc taccttaaca gaaagcgggg acctgcttat agccatggca
    83161 gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa
    83221 aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt
    83281 gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga
    83341 acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag
    83401 cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa
    83461 acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt
    83521 ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt
    83581 tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc
    83641 tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa
    83701 aagtgccgat attgactgaa tatgaagaag caattggggc taagattaaa gaagcctact
    83761 tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag
    83821 attttacagc tatcaaaaat tttgaattag gaatagagtc atcgtgacag cagaaagtgt
    83881 gcaaaaagac tttgtttcta gaaccttctt tccaaaatta acaatcaatg aagatccagt
    83941 atgtggatca gcacattcta atcttattcc ttattgggca gaaagaatga ataaaacaaa
    84001 attgtcagct tatcaaatgt catcaagggg cggggatcta gaatgtgaat taaaagaaga
    84061 tcgtgtgata ataggaggaa aagcagtttt gttttctaaa ggtacctcgt ttatttgatt
    84121 tctttcaaat gaattaaaaa taaattggtg gagagtggca gccttatagc tacaatggtt
    84181 gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt ttgcgtcttt ttttaattat
    84241 tggtgtttgt tttttattca atcaagagta aaatgcccca tactaactat cctcggatga
    84301 cttcgatttt ataaccatca gggtcaatga caaagtagta agaaggcgta gtgccaggta
    84361 atccttttaa gtcagtaatc tcaagacctg cagctttatg ttgctcgtgt aatttttcta
    84421 aatcatcagc tgcaatagcg atatgaccat agccgtctcc tagatcatac gcaccatgat
    84481 catagttgta agtcaactct aattcatagt catctccagg aagtgtcaaa taaacaagtg
    84541 taaatttgtt ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat gccttttgat
    84601 aaaattcgat tgatgcttca agatctttca cccgaacaca tgtgtgagcc attttcataa
    84661 attcatctct ccttttcctt ttactcaatc ataacaaaaa gaagtaagaa aatagttgat
    84721 ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata gaaaataata ctgttaaggt
    84781 ggaaacaatt catggataaa ccaatttttg gtgaaaaaaa gcaatctgct aattatcaaa
    84841 cccgttatgc agcatatatt gtcatagaaa gagacaagcg gattgcggtg atcgaggctc
    84901 caaatggtgc ttttttcctt cctggtgggg aaatcgaagg agaagagacg aaagaagaag
    84961 cgattgtccg tgagctgata gaagaaatgg gaattgtggc agaaatttct tcttatctgg
    85021 gagaagctga tgagtatttt tactcgaatt atcgtgcaac ttattattat aatccaggat
    85081 atttctttgt tgctgattct tggaagcgta taggagaacc aactgaaaag accaataaaa
    85141 tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc caccgatggg
    85201 cagtcgagag atggatcgaa aaaaataaaa actgaaagaa agcgagtaga tcagctttct
    85261 ctcagttttt ttatttatta cagtacatat tttttgattg cttcagctac accgtgctcg
    85321 tcgtttgatg aagtgatgac atttgctgct tctttgacaa gagaaacggc atttcccatt
    85381 gctactccta gaccagcata ttcaatcatg gataagtcgt tctcgttatc tccgatagcc
    85441 atgatttcat cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta
    85501 cttgcttctt tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta
    85561 tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc
    85621 atcatcattt tgacgatgtt catgtctggt gtcatttctt caggtgttcg atattttagc
    85681 ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg attaggcgtg
    85741 tagatcgctt tgtcatcgat cgaatggaga tgactgccga cacggcgtgc catcacttcg
    85801 atctctagga aatcttcatg agtcagtccg tgacgggcga tgattttacc tgatttcgtt
    85861 tgttgtacga gtgccccgtt ataagtaata acgtaatcat tatcttgaaa aaggttcaac
    85921 tcgtttaact gttcctggac acctggtaag ggacggccag tgcataaaac gatcgaaaca
    85981 ccttttgcaa cagcagcggc aatcgcttct tttacttctt ttgttacttc tcttttctca
    86041 tttaataaag taccatcgat gtcgatagcg actagtttga tagacataat ttcctccaat
    86101 atattttaat ttatttcaat caatgcgcca ttacggatat ggctggcaaa ttcttgataa
    86161 gtttcgtcaa ataaatcata atgatcacgc aaggagggat caaccatttc ttttggaaag
    86221 aaaaatcgct catctccttg ttcttgtccc gctaatgcag caacaagctg actaacctgt
    86281 gataattcta tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct
    86341 tgttctgggc gataaaaatc ataaggcaga tcgtagctag aattcacagc agtgtaataa
    86401 acagggttat agccaacctt ttctactaac aaagtgagtt cttggactaa ttttgaatcc
    86461 cggttgccag aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg tttagctaaa
    86521 tcatttaaaa taggatcagc agattcagac cattgagtaa agtaagtgcc caagacccca
    86581 tcatcaagtt tgaggtattc ttctaaagta aagcttccgt caagaaatgg cagaagtaat
    86641 tgagaatgga gttcaaaact gctggcatct tcttgataca actcgtgtgc gcgatgaagc
    86701 agatgctcca aaatcacttc cataccacgg gaaacaggat ggaagtacac ttgcacatac
    86761 atttgatatc ggctaacgat ataatcttcc actgcatgca tgccactcat ggaaaaagcg
    86821 atcccgcctt cataaggacg aatcacacgt aatattcggg taagatcaaa tgttccatat
    86881 tctgttccag tgaaataagc atcccgaagc aaatagtcca ttcgatctgc atcgatctga
    86941 ctagaaatca tttggacaac ttgtgggttg gggtaagttt tttggatcac actagctacc
    87001 ttttcaggaa aaccttcttc tactcggtta agaatctgat acacttctgt ttctggagaa
    87061 gtgatgatct ctacagtgat ggcttcatga ttcgtatgga agatatgttc aaatgtatga
    87121 gagtaaggac catgaccgac atcatgcaac agtgccgcac ataaagtcac cagccgttcc
    87181 tgatcatccc agcctccgtt gcctattttt tcttttgaaa aatttcgaga aaagatatcg
    87241 cagattcgtc ttgaaatctc ataaacgcct aatgaatggg taaaacggct atgttctgct
    87301 ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc gtcgtaaacg ttggacttct
    87361 ttcgaattga tcaaatctaa gattacttta tgctggacat ggatatagtt atggactgga
    87421 tcacgaaaaa ctttttccat aggcagcatt tgatatttat aaggggttgt cataatgtag
    87481 ctcctttatg ttattacgtt gttccattct ctggatttgg ttgttccatt gctccagctg
    87541 gatttctttt tttagccagt cggtagaatc ctgtaaatca cttgcagaat acagcttttc
    87601 aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa aggggttggt caagcagttc
    87661 ttcaagtgtt gccattgtag caggatccac tgcaggatag ccatttgtac caaagtcttc
    87721 ttttaaagca gattgataaa atcgcttcat gacttgcccg cgtttttcct gattaccgtt
    87781 gacacttaag tacatcataa cagaaatccc atttttgatc cgacgctgag cgataccggc
    87841 aaattttttg ccatggatgc ttaaatcata agttccagga caataagaat gggtgatttc
    87901 gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc aataggagca tttgctcgta
    87961 agcgtcatct atggttattt tttttgtgtc actttgaggg aaaatcaaag aaatatttaa
    88021 aattccttca tctgatacga tccctaatcc acctgaattt ctgatcactg gagcgtaacc
    88081 tgcatctttc agtgtttgga tcccgtcact tagaaaagga gttcgaacat ccttcatacc
    88141 taagatgact gtgcagtcat attgccagaa atgtaaaaag atttgctggt gttctttaga
    88201 atattccgtt aatatatctg ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg
    88261 ctgatctagt cgaatcgcag ggttcattca accggctcct ttgtataaat tcatattttc
    88321 tacaatatta tactctaaaa tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg
    88381 gacaagttgg ttgacaaaac aaaagaaact aggaacaatg tagctatgag aagtaaagga
    88441 gaatgaataa tggaattatc acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta
    88501 aaacagcaag gggaagtgca agatttttat tttgatttga aagggcaaat ggtgacaatt
    88561 ggcgatacgc tgtacatacg ttataaagag attcaggagg agacgggcga tgagattcct
    88621 gtaacgatca aattagttcc agatggtcat gtacagctga tccgtgcagg agaaatgcgg
    88681 atgcgtttga aatttggcta taaagaacgg ttagaaacat cctatcgaac accatacggt
    88741 atgatccaga ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca
    88801 gcagggaagg ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat cggcgaatat
    88861 tatttgacct tggattttac agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga
    88921 ttttgtatga taaggagtag actgtgaaag gacgtgtcct gattggaaat tagtgtattt
    88981 gaaggtgcaa acaaaagcga attatcaatg atcgaagtag cacacgcgat cttagaacaa
    89041 cgtggcgatg tcatggattt ttctgattta gctaaccaga tccaaaacta tcttgagaaa
    89101 tcagacagtg aaattcgtga ttcactcgca caattttata ctgacttgaa tattgacggc
    89161 agctttatct cattagggga taatcgttgg ggcttgcgtt catggtatgc tatcgattcg
    89221 atcgatgaag aagtaaacca tggaatggac gaagaggatg aagatactcc acgtcgccgt
    89281 aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag acgcgatcga ttacaatgac
    89341 gatgacccag aagatacaga tctgacagaa gaagatgacg atgatctgtt tgatgacgat
    89401 gatgatgaag atgaagaaat cgctgcttat aattctgact tgcaagaaat cggagcagat
    89461 gacccgacag atgatgaaga agacttgcca ggaaatatcg aagaagattt aagtatcata
    89521 gacgaagacg atgatgatga agattatgaa gaggaagaat tttctgactc tgaaaaagaa
    89581 taaacattat tttcttacga gaggttgtga cagagacgat cagcctatca gaataaggag
    89641 gtgtgacacg atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat
    89701 aaaaaataaa aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa
    89761 aggagtggag aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc
    89821 acctgctgaa tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt
    89881 acgtacatga taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccttga
    89941 actattttga aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga
    90001 tccgcacggc attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa
    90061 ttcaagatat cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt
    90121 tagataaaat agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc
    90181 gtttgaaaga aattgcaact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt
    90241 accgctcatt gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat
    90301 tcccaaaaag aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa
    90361 gggcttaaag aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca
    90421 aggcggagaa agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca
    90481 acaagtagcc ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta
    90541 ggatcagtca ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat
    90601 attcaggtac cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta
    90661 aatcaaaaat actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga
    90721 aattttgaaa cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac
    90781 cggtttctcg tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa
    90841 agatacgaca caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa
    90901 gatgacactt atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc
    90961 aaggaagatc catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa
    91021 aaaccaacac ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt
    91081 ttgacttttt ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt
    91141 cctaagtccg tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga
    91201 agtagttgcg actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa
    91261 aaagagaaag aagagatata gtgaaaagaa attatttagt catcagtatt attgctttat
    91321 ccgtgctttt gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac
    91381 aaacacactc ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta
    91441 aaagccaatc aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg
    91501 tacaaagtca atcatcacaa tcaacacaag acagtacaga agcaatccaa gacccgactg
    91561 tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc gaccacaacg ccgcagcttc
    91621 tgcgaatctt aaaagaaaaa aacgtgcatg tcactttttt cgtcttagga gaaaatacgg
    91681 cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca
    91741 catacgatca tcagaatctg gccactcttt ctgcccagtc gatgacggag gaagtgacga
    91801 aagcagacac tgagatcaaa aaaagtcact ggcaaaaccc ccgcttttgt tcgtccgccc
    91861 tatggttctg tcacgaatgt aggagcaacg atcatccagc gaccgatcat cgaatggtct
    91921 gttgattcag aagattggaa gacaaggaat cctgatttga ttttgcagaa gatccaagca
    91981 acagtatacg atgggtcaat tattttattc catgatatct atcctgaaac gattcgtgcc
    92041 gttcctcaag tgatcgatta tctgaaagag caaggctatc gcatcacgac agttagcgat
    92101 ttattagggc atccaacggc tgtcgagaac tattatggca gaaacgatca tcggccagtt
    92161 cagtaagtga gacaagtatc tttaataaat aacacatttt aataagtagt tgaacacata
    92221 actaccttac ttgcaaaggc ttaacaatat ctatacagat acaacaaaaa actgtccacc
    92281 aattctcttt ggcggacagt tttcacttaa aaataaagaa gcaactattc gtaaattatg
    92341 ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc ccgtctcaag aaccggaatc
    92401 ttacgtgata tccactacac taaggacgca agcactggga taattgtaac taaacagata
    92461 aaaaattaca aggggattgg acaaaaaaat gaaatttgag caaaattttt cacaaaaaca
    92521 gcagcagaca cagaaactag ccatgacgca acagttgcaa cagtccatcc aagttttaca
    92581 atataacagt gaggagctgt tggcattcgt tgagaatcaa gcaatggaaa atccattggt
    92641 ggaagttgtt gagccggaat ggcagccaga ttacataaaa gcttcttcat cttcttatga
    92701 aggagaagag acaaattacc taaatcagat tcctgataca aaagggtctt tatttgattc
    92761 attgatcgaa caagtccatt tgaattaccg agatactttt ttgaggaaaa tcgttcttta
    92821 tcttgtagaa tacatcgatt tgaacggatt tttgacgatt agtttagaag aagccggaaa
    92881 agagattgga gctacgccta tccaaatgct cgatgcgttg actctgattc agcaattaga
    92941 accagctgga gtaggtgcac gtagtttgca agaatgtctc atgcttcaaa cagaacgaga
    93001 tgattatgcg ccggaattag cttatatcgt gttggaagaa tgttttgagg aactggtgga
    93061 gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat ctacatgctg ttcagcagat
    93121 attcgactat ttacaaacgt tgagtccaag ccctggccgg attttcgacc gtagcagcga
    93181 attgtacatt attcctgatg tccgggtatt agtggacgac gataaaaatg tacaagttat
    93241 ctcaaaccga aaaaatcagc caaatatacg ctttcaagag agttacttca aacaaatgtc
    93301 acaacaagca gataaagaaa ctgaaaatta tttaaaagaa cggaaacagg aattcgaatg
    93361 gttgaagaaa acgatcctcc aacgtgggga tacaattttg cgggttgctc aagtcattgt
    93421 ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca atcaagcctc ttacgttaaa
    93481 ggaagttgca actgagatag atgtgcatga atccacggtc agtcgtgcag tcaacgggaa
    93541 atatttggaa acagatttcg gtgtatttga attaaaaaaa tttttcacta cacgaattcc
    93601 tacgaatagt acagaacaga cagaagacct ttcagcagat acagcgaaga aaaagctgca
    93661 ggaattagtc gatcaagaag acaaaaacaa gccgttgtcg gaccagaaat tggttgaatt
    93721 gctgaaaaaa gatgagatag cgatctcaag acgaacagta gcaaaatatc gtgatttatt
    93781 aggaatcccg agttcatcaa aaagaaaacg ttatgataac taatcagtca aaagactaga
    93841 aaaagtattt cactttttct agtcttttta gttgaaaaac gagagcaaaa ctgttagact
    93901 tacagtcgtg aggttgagat ttccctttat tgatgtgaaa ttcgaatagt taatttcttt
    93961 ttttatcgta tatgggtcgt tttaagtcta cattggacgt aaagtgtcca actaaggaga
    94021 tcgctatgct ggatgaaata aaaatgattg aatctgttgc accagatatt atcgaagtgt
    94081 tgcaagaacg ctataaaatt ttacgaaata tctattggat gcagccgatt gggcgtcgaa
    94141 gtttatcgga aagtatgggg atcacagaac gagtcttacg aacagaaaca gaccttttga
    94201 aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac gctgacaact aagggcgaag
    94261 aagtttatca aagccttgaa aacttcatgg atcagctttt aggtacgcat caaaccgaac
    94321 aacagttggc agaatatttt ggtaaccaac gctgtatcgt tgtttcagga aacagcgaag
    94381 agcagaccaa agtggctgat gcattcggcg aagcgctgag cgaagcctta gaccgactac
    94441 ttccagaagg cgagaatatc atcgctgtga tgggaggaac tactatggct gtggtagccg
    94501 aacaattgtc taatctggaa aataagaaac ggcataacct gtttgtccct gcaagaggcg
    94561 ggattggtga agcgataact gtacaggcaa attctgtcag tgcaagaatg gctgctaaag
    94621 caaacggtaa ccaccgcgca ctttatgttc cggaacaatt aagtctggca acttacaatt
    94681 ctttactcaa tgagccatct atccaagaag tcttaaactt gattagtgaa gcgaattgtg
    94741 ttattcatag tattgggcgt gcattacaca tggcagcacg tcgtaagatg actgaaaaag
    94801 aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc ttttggctac tttttcaatg
    94861 aaaaaggtga agtcgtttat aaggtcccta gaatcggcat agaattgcaa gatttggaaa
    94921 aagtcccgat catcatggca atcgcgggcg gaaaatcaaa agctaaagca attcgagctt
    94981 atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga tgaagctgct gcaaatgaga
    95041 ttttaaaagg ggtaaccctt taaaataaat atttttttga ttttcataag gaggaaatct
    95101 taaatgacag ttaaagtagg tattaatggt tttggacgta ttggacgtct agcattccgt
    95161 cgtatccaag atgtagatgg aatcgaagta gtagcaatca acgacttgac agatgctaaa
    95221 atgttggcac acttgttaaa atacgacaca actcaaggac gtttcaacgg aacagttgaa
    95281 gttcatgaag gttcattcaa cgtaaacgga aaagaagtta aagtattagc taaccgcaac
    95341 ccagaagaat taccatgggg cgaactaggc gtagatatcg ttctagaatg tactggtttc
    95401 ttcacttcta aatcagcagc tgaaaaacat ttaacagctg gtgctaaacg cgttgttatc
    95461 tctgctcctg gcggaaacga tgttccaaca atcgtttata acacaaacca cgaaacatta
    95521 actggtaaag aaacagttat ctcaggtgct tcatgtacta ctaactgttt agctcctatg
    95581 gctaaaacat tgaacgacaa atttggtgtt gttgaaggat taatgacaac tatccacgct
    95641 tacacaggtg accaaatgac tctagacgga cctcatccta aaggtgactt ccgccgtgca
    95701 cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg ctgctaaagc tatcggttta
    95761 gtaattcccg aattgaacgg taaattagac ggagctgctc aacgtgttcc tgtaccaaca
    95821 ggttcattaa cagaattagt aacagttctt gaaaaagaag taactgttga cgaaatcaat
    95881 gcagcaatga aagaagcttc aaacgaatct tacggataca acacagacga aatcgtttcc
    95941 tcagatatcg ttggtatgac ttacggttca ttatttgatg ctacacaaac taaagtaatg
    96001 acagttggcg acaaacaatt agttaaaact gttgcttggt acgacaacga aatgtcatac
    96061 actgcacaat tagtacgtac tttagaatac ttcgctaact tataagatag acaaacgtac
    96121 aagtgtaaat tatagacatg tacatttaaa agcggggaag caatcgcgct tcttccgctt
    96181 tttttgataa taagtttatt caggaggaaa taaaatggct aagaaaacag tgaaagatat
    96241 cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc aacgttcctt tgaaagacgg
    96301 cgtgatcact gacgacactc gtatcaaagc agcattgcca acaatcaact acgttctaga
    96361 acaaggcgga aaagcaatcc ttttctctca ccttggacgt gtaaaaacag aagaagataa
    96421 agaaggaaaa tctttagctc cagttgcaaa acgtctaggc gaattattag gtaaagaagt
    96481 aacattcgta cctgaaactc gcggagaaca attagaagaa gcaatccgca acatgaatga
    96541 cggcgacgtt gtcgtattcg aaaatacacg ttttgaagat attgacggta aaaaagaaag
    96601 caaaaatgat acagaactag gtaaatactg ggcttcatta ggcgatgtat ttgttaacga
    96661 tgctttcggt acagctcacc gtgcacatgc ttctaacgta ggtatcgctt caactggtat
    96721 cccaacagtt gctggattct tgatggaaaa agaaatcaag ttcatcggtg aagcagtaga
    96781 agaaccaaaa cgcccaatga ttgcaatcct tggtggcgca aaagtttctg ataaaatcgg
    96841 cgtaatcgaa aacttgattc ctaaagcaga taaaatctta atcggtggcg gaatgactta
    96901 cacattctac gaagcaaaag gaatcaagat cggtaactct ctagtagaag ctgataaagt
    96961 agaactagca aaagaattaa tcgaaaaagc aggcgacaaa cttgtcttgc caatcgacaa
    97021 tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa gtaatcgaag gagacattcc
    97081 tgatggtttg atggctttag acatcggccc agcatcagta aaattatttg ctgatacatt
    97141 acaaggtgca aaaacagttg tttggaacgg acctatgggt gtatttgaaa tgagtaactt
    97201 tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac ttagaaggcg caacaacaat
    97261 catcggtggc ggagactcag ctgcagcagc agaacaatta ggattcgctg acaaattcac
    97321 acacatctca acaggtggtg gcgcaagctt ggaattgctt gaaggtaaag aattaccagg
    97381 actagcagca atcaacgata aataaacgac caagtttgct caattctgac gagtaatcga
    97441 tcaaaaggtg aagggagttt tcatgactcc ctagccattt ttcaataaat taaggagtgt
    97501 gttcctcaat gcgtaaacct attatcgcag gtaactggaa aatgaacaaa actgcatcag
    97561 aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc ttcaaatgac aaagttgatt
    97621 cagtcatcgg atctcctgca ttattcttac aagaattaat gacatcagct gaaggtacag
    97681 aactaaaaat ctcagcacaa aactgctact gggaaaactc aggtgcgttc actggtgaaa
    97741 cttcaccagc agctcttgct gatctaggtg ttcattatgt gatcatcgga cattcagaac
    97801 gtcgtgaata tttccatgaa acagacgaag acatcaataa aaaagcaaaa gctatctttg
    97861 caaatggtat gacaccaatc ctatgctgcg gcgaatcttt agaaacctat gaagcaggca
    97921 aaactgctga atggatcgaa ggacaaatta cagctggttt agctgatctt tcagctgaac
    97981 aagtgtcaaa catggttatt gcttatgaac caatctgggc aatcggtact ggtaaatcag
    98041 ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg tcaaacagtt gaaaaacttt
    98101 atggaaaaga agtatcagaa gcagttcgta tccaatacgg cggttctgta aaaccagaaa
    98161 atattgctga atacatggca aaagaaaatg ttgacggtgc tttagtcggt ggcgcaagct
    98221 tgcaagcaga ttcattcttg tcattgttag acgctgtaaa ataattgttg aaaatttgtg
    98281 aaaaatgcaa gaaatgtttg cactaatgag caaaattaac taaaatcgta tatgagggaa
    98341 atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca tgtcaattat tactgatgtt
    98401 tacgctcgcg agatcttaga ctcacgtggt aacccaacaa tcgaagtaga agtatacaca
    98461 gaaagcggag cttttggccg cggaatggtt ccttcaggtg cttcaactgg tgaatacgaa
    98521 gcagttgaat tacgtgacgg agacaaagct cgttatggcg gtaaaggtgt aactaaagct
    98581 gtcgacaacg taaacaacat cattgctgaa gcaatcattg gctacgatgt tcgtgaccaa
    98641 atggctatcg acaaagctat gatcgcttta gacggaactc ctaacaaagg taaattaggt
    98701 gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg cagctgctga ctacctagaa
    98761 gtacctttat accactacct aggcggattc aacacaaaag tattgccaac accaatgatg
    98821 aacatcatca acggcggatc acatgctgat aactcaatcg acttccaaga attcatgatc
    98881 atgcctgttg gcgctccaac attcaaagaa gctctacgta tgggtgctga agtattccac
    98941 gcattagctt caatcttgaa agctcgcgga ttagcaactt ctgttggtga cgaaggtgga
    99001 tttgctccaa accttggttc aaacgaagaa ggttttgaag taatcatcga agcaatcgaa
    99061 aaagctggct atgtacctgg taaagacgtt gttcttgcta tggatgctgc ttcttcagaa
    99121 ttctacgaca aagaaaaagg tgtttacgta ctagctgact caggcgaagg cgaaaaaaca
    99181 actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta aatatccaat catctcaatc
    99241 gaagatggat tagacgaaaa cgactgggac ggattcaaga aattaactga cgtattaggc
    99301 gacaaagttc aattagttgg tgacgacttg ttcgtaacaa acactcaaaa attatctgaa
    99361 ggtatcgaaa aaggaattgc taactcaatc cttatcaaag tgaaccaaat cggtacatta
    99421 acagaaactt tcgaagctat cgaaatggct aaagaagctg gctacacagc agttgtatct
    99481 caccgttctg gtgaaacaga agattcaaca atctctgata tcgctgttgc aacaaacgct
    99541 ggccaaatca aaactggttc tctatcacgt actgaccgta ttgctaaata caaccaatta
    99601 ctacgtatcg aagaccaact tggtgaagtt gcagaataca aaggtttgaa atctttctac
    99661 aacttaaaaa acaaataatt cttttctaat gaagatacat gatcgactat tgaaagatag
    99721 tcgagtatga tcacttagtt aagtaatcga actctctact tgctgaaat
//
LOCUS       DAPBAB010000005        89745 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPBAB010000005 DAPBAB010000000
VERSION     DAPBAB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 89745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.005"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..1253)
                     /locus_tag="RD444_000458"
     CDS             complement(<1..1253)
                     /locus_tag="RD444_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7547858.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNE"
     gene            complement(1681..2754)
                     /locus_tag="RD444_000459"
     CDS             complement(1681..2754)
                     /locus_tag="RD444_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7547859.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(2751..3578)
                     /locus_tag="RD444_000460"
     CDS             complement(2751..3578)
                     /locus_tag="RD444_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547860.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(3579..4385)
                     /locus_tag="RD444_000461"
     CDS             complement(3579..4385)
                     /locus_tag="RD444_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547861.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(4397..5482)
                     /locus_tag="RD444_000462"
     CDS             complement(4397..5482)
                     /locus_tag="RD444_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547862.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(5499..6041)
                     /locus_tag="RD444_000463"
     CDS             complement(5499..6041)
                     /locus_tag="RD444_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7547863.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            6299..7288
                     /locus_tag="RD444_000464"
     CDS             6299..7288
                     /locus_tag="RD444_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDT7547864.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7285..8022
                     /locus_tag="RD444_000465"
     CDS             7285..8022
                     /locus_tag="RD444_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDT7547865.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            8019..8570
                     /gene="coaC"
                     /locus_tag="RD444_000466"
     CDS             8019..8570
                     /gene="coaC"
                     /locus_tag="RD444_000466"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDT7547866.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            8586..9218
                     /locus_tag="RD444_000467"
     CDS             8586..9218
                     /locus_tag="RD444_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7547867.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            9303..9950
                     /locus_tag="RD444_000468"
     CDS             9303..9950
                     /locus_tag="RD444_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7547868.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            9955..10938
                     /locus_tag="RD444_000469"
     CDS             9955..10938
                     /locus_tag="RD444_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDT7547869.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(11341..12855)
                     /locus_tag="RD444_000470"
     CDS             complement(11341..12855)
                     /locus_tag="RD444_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDT7547870.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            12993..13799
                     /locus_tag="RD444_000471"
     CDS             12993..13799
                     /locus_tag="RD444_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547871.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(13974..14543)
                     /locus_tag="RD444_000472"
     CDS             complement(13974..14543)
                     /locus_tag="RD444_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7547872.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(14626..16686)
                     /locus_tag="RD444_000473"
     CDS             complement(14626..16686)
                     /locus_tag="RD444_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDT7547873.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(16963..18441)
                     /locus_tag="RD444_000474"
     CDS             complement(16963..18441)
                     /locus_tag="RD444_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7547874.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(18739..19674)
                     /gene="murB"
                     /locus_tag="RD444_000475"
     CDS             complement(18739..19674)
                     /gene="murB"
                     /locus_tag="RD444_000475"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDT7547875.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(19747..20667)
                     /locus_tag="RD444_000476"
     CDS             complement(19747..20667)
                     /locus_tag="RD444_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7547876.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDKPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            20780..21532
                     /gene="xth"
                     /locus_tag="RD444_000477"
     CDS             20780..21532
                     /gene="xth"
                     /locus_tag="RD444_000477"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDT7547877.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            22396..22929
                     /locus_tag="RD444_000478"
     CDS             22396..22929
                     /locus_tag="RD444_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDT7547878.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(23419..23943)
                     /locus_tag="RD444_000479"
     CDS             complement(23419..23943)
                     /locus_tag="RD444_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7547879.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(23936..24409)
                     /gene="tsaE"
                     /locus_tag="RD444_000480"
     CDS             complement(23936..24409)
                     /gene="tsaE"
                     /locus_tag="RD444_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDT7547880.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(24532..25512)
                     /gene="pta"
                     /locus_tag="RD444_000481"
     CDS             complement(24532..25512)
                     /gene="pta"
                     /locus_tag="RD444_000481"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDT7547881.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(25665..26345)
                     /locus_tag="RD444_000482"
     CDS             complement(25665..26345)
                     /locus_tag="RD444_000482"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDT7547882.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            26471..27340
                     /locus_tag="RD444_000483"
     CDS             26471..27340
                     /locus_tag="RD444_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7547883.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            27366..27881
                     /locus_tag="RD444_000484"
     CDS             27366..27881
                     /locus_tag="RD444_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547884.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(27928..28521)
                     /locus_tag="RD444_000485"
     CDS             complement(27928..28521)
                     /locus_tag="RD444_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7547885.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(28546..28725)
                     /locus_tag="RD444_000486"
     CDS             complement(28546..28725)
                     /locus_tag="RD444_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547886.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(28802..29311)
                     /locus_tag="RD444_000487"
     CDS             complement(28802..29311)
                     /locus_tag="RD444_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547887.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(29407..29787)
                     /locus_tag="RD444_000488"
     CDS             complement(29407..29787)
                     /locus_tag="RD444_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7547888.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            30117..30866
                     /locus_tag="RD444_000489"
     CDS             30117..30866
                     /locus_tag="RD444_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDT7547889.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(31050..32327)
                     /locus_tag="RD444_000490"
     CDS             complement(31050..32327)
                     /locus_tag="RD444_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547890.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(32406..33050)
                     /gene="pcp"
                     /locus_tag="RD444_000491"
     CDS             complement(32406..33050)
                     /gene="pcp"
                     /locus_tag="RD444_000491"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDT7547891.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(33249..34193)
                     /locus_tag="RD444_000492"
     CDS             complement(33249..34193)
                     /locus_tag="RD444_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDT7547892.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(34195..34881)
                     /locus_tag="RD444_000493"
     CDS             complement(34195..34881)
                     /locus_tag="RD444_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDT7547893.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            35355..35675
                     /locus_tag="RD444_000494"
     CDS             35355..35675
                     /locus_tag="RD444_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDT7547894.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            35829..36734
                     /locus_tag="RD444_000495"
     CDS             35829..36734
                     /locus_tag="RD444_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7547895.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            36731..37504
                     /locus_tag="RD444_000496"
     CDS             36731..37504
                     /locus_tag="RD444_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7547896.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(37685..39109)
                     /locus_tag="RD444_000497"
     CDS             complement(37685..39109)
                     /locus_tag="RD444_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7547897.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            39320..39766
                     /gene="nrdI"
                     /locus_tag="RD444_000498"
     CDS             39320..39766
                     /gene="nrdI"
                     /locus_tag="RD444_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDT7547898.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(39831..41813)
                     /locus_tag="RD444_000499"
     CDS             complement(39831..41813)
                     /locus_tag="RD444_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7547899.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(41827..42723)
                     /gene="murQ"
                     /locus_tag="RD444_000500"
     CDS             complement(41827..42723)
                     /gene="murQ"
                     /locus_tag="RD444_000500"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDT7547900.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(42726..43787)
                     /locus_tag="RD444_000501"
     CDS             complement(42726..43787)
                     /locus_tag="RD444_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7547901.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(43998..44525)
                     /locus_tag="RD444_000502"
     CDS             complement(43998..44525)
                     /locus_tag="RD444_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7547902.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(44592..46094)
                     /gene="eat(A)"
                     /locus_tag="RD444_000503"
     CDS             complement(44592..46094)
                     /gene="eat(A)"
                     /locus_tag="RD444_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDT7547903.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(46352..47473)
                     /locus_tag="RD444_000504"
     CDS             complement(46352..47473)
                     /locus_tag="RD444_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7547904.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            47598..48539
                     /locus_tag="RD444_000505"
     CDS             47598..48539
                     /locus_tag="RD444_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDT7547905.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYNPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(48689..49585)
                     /locus_tag="RD444_000506"
     CDS             complement(48689..49585)
                     /locus_tag="RD444_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDT7547906.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            49854..51194
                     /locus_tag="RD444_000507"
     CDS             49854..51194
                     /locus_tag="RD444_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7547907.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVIVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            51412..51855
                     /locus_tag="RD444_000508"
     CDS             51412..51855
                     /locus_tag="RD444_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDT7547908.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(52120..53538)
                     /gene="nhaC"
                     /locus_tag="RD444_000509"
     CDS             complement(52120..53538)
                     /gene="nhaC"
                     /locus_tag="RD444_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDT7547909.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(53941..55164)
                     /locus_tag="RD444_000510"
     CDS             complement(53941..55164)
                     /locus_tag="RD444_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDT7547910.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(55195..56034)
                     /locus_tag="RD444_000511"
     CDS             complement(55195..56034)
                     /locus_tag="RD444_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDT7547911.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(56051..56731)
                     /locus_tag="RD444_000512"
     CDS             complement(56051..56731)
                     /locus_tag="RD444_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547912.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(56733..57806)
                     /locus_tag="RD444_000513"
     CDS             complement(56733..57806)
                     /locus_tag="RD444_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7547913.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(57862..58100)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(58142..58375)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(58452..59123)
                     /locus_tag="RD444_000514"
     CDS             complement(58452..59123)
                     /locus_tag="RD444_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7547914.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(59210..61888)
                     /gene="mgtA"
                     /locus_tag="RD444_000515"
     CDS             complement(59210..61888)
                     /gene="mgtA"
                     /locus_tag="RD444_000515"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDT7547915.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIHRFQELL"
     gene            62547..62984
                     /gene="ndk"
                     /locus_tag="RD444_000516"
     CDS             62547..62984
                     /gene="ndk"
                     /locus_tag="RD444_000516"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDT7547916.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(63006..63872)
                     /locus_tag="RD444_000517"
     CDS             complement(63006..63872)
                     /locus_tag="RD444_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7547917.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(64032..65426)
                     /locus_tag="RD444_000518"
                     /pseudo
     CDS             complement(64032..65426)
                     /locus_tag="RD444_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            65521..66480
                     /locus_tag="RD444_000519"
     CDS             65521..66480
                     /locus_tag="RD444_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7547918.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLSGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(66489..67190)
                     /locus_tag="RD444_000520"
                     /pseudo
     CDS             complement(66489..67190)
                     /locus_tag="RD444_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            complement(67666..68289)
                     /locus_tag="RD444_000521"
     CDS             complement(67666..68289)
                     /locus_tag="RD444_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547919.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(68448..69845)
                     /locus_tag="RD444_000522"
     CDS             complement(68448..69845)
                     /locus_tag="RD444_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7547920.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(69877..70848)
                     /locus_tag="RD444_000523"
     CDS             complement(69877..70848)
                     /locus_tag="RD444_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7547921.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            71222..71647
                     /locus_tag="RD444_000524"
     CDS             71222..71647
                     /locus_tag="RD444_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547922.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(72144..72845)
                     /locus_tag="RD444_000525"
     CDS             complement(72144..72845)
                     /locus_tag="RD444_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7547923.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(72861..73301)
                     /locus_tag="RD444_000526"
     CDS             complement(72861..73301)
                     /locus_tag="RD444_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDT7547924.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(73301..73876)
                     /locus_tag="RD444_000527"
     CDS             complement(73301..73876)
                     /locus_tag="RD444_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDT7547925.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(74724..75203)
                     /locus_tag="RD444_000528"
                     /pseudo
     CDS             complement(74724..75203)
                     /locus_tag="RD444_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(75362..76063)
                     /locus_tag="RD444_000529"
     CDS             complement(75362..76063)
                     /locus_tag="RD444_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7547926.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(76171..77613)
                     /locus_tag="RD444_000530"
     CDS             complement(76171..77613)
                     /locus_tag="RD444_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7547927.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(77627..79493)
                     /locus_tag="RD444_000531"
                     /pseudo
     CDS             complement(77627..79493)
                     /locus_tag="RD444_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
     gene            complement(79614..80466)
                     /locus_tag="RD444_000532"
                     /pseudo
     CDS             complement(79614..80466)
                     /locus_tag="RD444_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(80776..81489)
                     /locus_tag="RD444_000533"
     CDS             complement(80776..81489)
                     /locus_tag="RD444_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDT7547928.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            complement(81557..81883)
                     /locus_tag="RD444_000534"
     CDS             complement(81557..81883)
                     /locus_tag="RD444_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDT7547929.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            complement(82005..82253)
                     /locus_tag="RD444_000535"
                     /pseudo
     CDS             complement(82005..82253)
                     /locus_tag="RD444_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(82554..83807)
                     /locus_tag="RD444_000536"
     CDS             complement(82554..83807)
                     /locus_tag="RD444_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7547930.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            83868..84608
                     /locus_tag="RD444_000537"
     CDS             83868..84608
                     /locus_tag="RD444_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7547931.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(84694..85452)
                     /locus_tag="RD444_000538"
                     /pseudo
     CDS             complement(84694..85452)
                     /locus_tag="RD444_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
     gene            complement(85449..86477)
                     /locus_tag="RD444_000539"
     CDS             complement(85449..86477)
                     /locus_tag="RD444_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDT7547932.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKAYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            complement(86497..89667)
                     /locus_tag="RD444_000540"
     CDS             complement(86497..89667)
                     /locus_tag="RD444_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7547933.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLYYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     NQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATK
                     IVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYDQRETKSPWILTIKLS
                     QPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQTDYSEYLNNGRAFKL
                     VLTKENQIAGNYSAEMTWTLADAPTE"
BASE COUNT        26034 a        18594 c        15578 g        29539 t
ORIGIN      
        1 ttttcattga aattaccttc atttatcagc tgagtaattt tttccaaaat cgaatcataa
       61 tcaaacggtt tgattcgatc cgtttttatc ttttgatttt tctttatcaa taaactagca
      121 atcggcaagc tttctacttc ggattccagc atatttaact gaatcaagat ctcatttttc
      181 ccaatcgtta atggttcttc ttgtgttcga ctatacacat ccgcacaact gattttaatg
      241 atattcttca gcttaccaat attcccacta aagggatgag ataacattaa agacaaggct
      301 tctcttgaaa caataatatc tttttgtaaa acatccgctt cattttgaaa aaataattga
      361 atcagctcta atcgttctcg aatggggcga tcttcaaaag ttggcaacat aacactaatt
      421 tgaatacgcc gatcaaaggt gtctaataaa taattttcac ctttttccgt cgtggcgaaa
      481 ataaaacgaa ccttagcaga atgccaattt acattttcac cgactggacg atagtttcca
      541 gtatcaataa atagaaagag cttttcttga ttttctttcg ataggcgatg gacctcatcg
      601 agaaaaagat agccgttgtt ggccgttgcc aataaaccat cggtatcctt ttcagccccc
      661 gtaaaggccc ccttcttgta tccaaacagt gttgccgaaa gcagttcggg attgtccgca
      721 taatcggcac agtttaagat agcaaatggt gcatcttttt caataatttg ctcatggata
      781 gcgtattcaa aaatttttgt ggctaaaaaa ctttttccaa cacccgtggc tccatttatt
      841 aatacactga gtccgttagg cggatatttg acagcggcta tacatttttg aatgacctct
      901 ctcagacttc cgtcatgacc aactacagaa tgaaaggctt cgtttatttg ctccgtatta
      961 tctacaaccg catattcatc taaaggcttc cacaaaacag gctttcccgg taatttctcg
     1021 acctttttat cttccactaa tctcgtcaaa taatggctgg cattctgacg agagatacct
     1081 agattcttcg aaattttctc ggcagtgaca gaagacatat tccctgttag tcgtatcagc
     1141 tcatcaaaaa ctcgatcttt agcgctcttt ttcatttttt cctccagttt aatgtgtcta
     1201 aaagcataat aaactatcgg ctgcaatatg tctattctat atttgtattc caatttttac
     1261 tttgaaacac aaatagtcga atatttgcct cagatatctg acaaaataat ttatctacca
     1321 atttagtttt tatctaatat gatttttatg ttttttcata gctacaaata ttgaactgga
     1381 ttcatatatt agttttatta atagattaca gaagggttaa gtatatttgc atagtttcca
     1441 attactttat ccttctgtaa ctttttcaat cttagattta ctagcccgtc tcgggcccta
     1501 ttttacaatg gttttgtcca cttttctctg gtagaaaaac gtggtagaaa aaataaaata
     1561 acacaaaaaa agcggcgttt catccgcttt tggtagaaga ttggaaaact cagatagaaa
     1621 aaggacgatt tttagctctt tagaacgttg ataagtcccc attcttactg agttttgcca
     1681 ttatctccta tacatcttga attctaagaa taggtcatta tagatgccta ggtatttcga
     1741 tcctagatct ttgtagactt caagatggga gatcgtgtca tcagatggat agaattgttc
     1801 gtccgacgaa atgtcttttg gcaataacgc ttttgcttct tcatttggtg tagaataacc
     1861 aatatactct gcattttgtg ccgcattttc cggttctagc ataaagttga tgaaatcata
     1921 ggcaccttct ttatttttca cggtttttgg cataacgata ttatcaaacc aaagattgga
     1981 tccttctggt gggatcacat aatgtaaatg ttcattttcc cacatcatat cggcagcttc
     2041 gccagaaaat gtcactgcca cggaggcttc ttcattgatc atatacattt tgatctcatc
     2101 tgccacgatt gctttgacat ttggtgttaa atgattcaat ttatttgctg cttctgtcag
     2161 ctgtgccata ttcttactat tcaaagaata gcctaaacta ttcaaagaaa gccccataac
     2221 ttctcgtgca ccgtcaatca gcatcaaact gtcttttaat tccggcttcc acaaatcatc
     2281 ccaatgctta atttgatttg catcaaagaa tttatcgtta tatacgatcc ctaaagttcc
     2341 ccaaaaatat ggaatcgaat actgattatc tggatcaaaa tccaagtcta aaaatcgtgg
     2401 atcaatatta tttaatcctt tgatcttcga atgatctaat ggaagtacta gtttttcttt
     2461 gatcattttt tggatcatat attcacttgg tatagcgatg tcgtagtttg tcccaccttg
     2521 ctggattttt gtgaacatcg cttcattaga atcaaatgtt tcataattca ctttgtatcc
     2581 atattctttt tcaaatttcg tgatcaaact aggatcgata taatctcccc agttatagat
     2641 ggtaagtacc tttgctccac tcatgccact tgttttttca agttgacgaa caccaagcag
     2701 cagcaccgca ataatgatga gaatacctaa aaacagagat tgtaattttt tcatcgcagc
     2761 cgtgccaccc cctcgcgtac tcttctgttt ttcttcagcc gttttggacg gttttcttta
     2821 ctaataaagt agtatccaat gaccaatatc atggagaaca agaaaactaa cgcacttagc
     2881 gcattgattt ctaaactgat tccttgtctt gcacgagaat agatctctac tgagagcgtc
     2941 gtaaagccat ttcctgtgac gaaaaatgta accgcaaaat catcaagaga ataggtaaat
     3001 gccataaaat atccagctat gattcctgga gaaagataag gcaaaatgat atttttgact
     3061 acttggaaat tattagcacc aaggtctcta gccgcatcga ccattgaatc gttcatttcc
     3121 tgcaattttg gcaagaccat caagacgacg attggaatgc tgaaagcaat atgggaaagc
     3181 aatacactct gaaaacctaa tccgaatcct agtagtgtaa agaaaatcaa gaaactagca
     3241 ccgataataa catcaggaga gactaataag atgttattca tgctcaacaa cgctgtccgt
     3301 gcacgtcgtt ttttcgtgta atagattccc atagccccaa atgtcccgat gatcgtggcg
     3361 atcaatgcag ataagaaagc cagcatgaac gtattcaata cgataatgat caaacgtgta
     3421 tcttcaaaaa cagcttcata attttcccaa gtaaatcctg taaagctgtt catcgttcct
     3481 ccttcattga aggaataaaa aatcagatag aagatcggtg catataagaa taaaaagacg
     3541 atccctaaat aaagatacga ccatttaaat tttttcattt atgagcgacc tccttttttc
     3601 ttctcgcctg tcaggagcat cacgatgaac atagcaatga ttaaaatcac accgattgta
     3661 gaacccatcc cccagttttg tgtaaccaag aaatgctgtt cgatagccgt tcctaaagtg
     3721 atcacccgat ttccgccaat cagacgagtc agcatgaata aagaaagaga aggaataaat
     3781 actgcttgga caccactttt cacaccattt aatgataacg gaaaaatcac tcgtcggaag
     3841 gtctcaaagc tattcgcgcc taaatctcta ctagcactga tcaaagaagg attcatctct
     3901 tccaatgcat tgaaaattgg cataatcata aatggaatct cgatatacgt tgccacgaac
     3961 agaaaactga agtctgtgaa taatatctgt ttcggcccga tcccgataaa atcaaagaaa
     4021 tgattcacgc tgccgtgcac actgaaaatc ccaataaatg cgtaagcttt aagcagcaga
     4081 ttcacccatg taggcaaaat aatcagcatc agccataatt gcttgtgctt cagttttgtt
     4141 aaaaaataag cagtcgggta actgatgatc agcgtgaaaa acgtgatcaa aaatgcatac
     4201 catacagaat tgatcgtcat agaaagcgat gtccccgacg taaaataaga tagataatta
     4261 ttcagcgtga attgcccatt catatcaaaa aaagattgat aaatgatcat taatacaggc
     4321 gcgatcacga ataaaccgag ccacaaaata taagggacgg aatagatcct gcgcattgtt
     4381 ttcattcttg tcccccctat tcttcgtagc tgtcaagacg tgcatcgaat tcttcttcag
     4441 actcgttgaa acgcatgaca tggatatctt ctggctcaaa ggccaatcct actttgcttc
     4501 cttctttagc ttttttcgtt gaatggacca tccattcatt ttgctgttca tcataacaga
     4561 tgatttcata atggacacca cgaaacagct gagtatcgac cgttacgact agttttcctt
     4621 tatctggtgt cgtgatcgtc aaatcctctg ggcgtaaaac aacttcaact ggttcattta
     4681 gacgcatccc gccatcgaca cattcaaatt gcttaccaac aaactcaact agattatctt
     4741 cgatcattac accgtccacg atattgctct ccccaacaaa atcagcaacg aaatgattga
     4801 ttggctcatc ataaatatct acgggtgtac cactttggac gattttccct ttattcatga
     4861 caaaaatctc atcactcatt gccaacgctt cttcttggtc atgagtaaca aaaataaacg
     4921 tgattcctaa tcgttgttgc agatcccgca gttcatactg catatctgta cgtagtttca
     4981 aatcaagcgc cgataacggt tcatctaata gcaatacttt cggttcattt acaattgctc
     5041 gtgcaatcgc tacacgctgt ctttgaccac cagacatttc actaatctcc cgtttttcgt
     5101 atccaggaag ctggaccata cgcaaagcat cttttacctt cttttcgata tctgatttgg
     5161 acattttctt gatcttcaat ccaaaagcaa cgttatcaaa aacattcata tgaggaaaaa
     5221 gtgcataatc ttggaagact gtatttactt gtcgcttatt agctggcaaa tcattcaacc
     5281 gtttcccttc aaaatagatg tccccttctg tagcttcggt gaatccagct ataatacgta
     5341 ggatcgttgt tttaccacag ccggaaggac caagcagtgt ataaaatttt ccttgttcga
     5401 tctcaaaact tacttttttt aatactgttt catcatcata gcgtttcact acttgatcaa
     5461 atgaaatgat tggtttcccc acgtttttac cccctatatt acaaatatga ttgcgttgca
     5521 acaatcaata cttttgttcg ttttttgaga tgattcctta attgatgttg ttttgtcgct
     5581 tgataataga tcgactgtcc tgcttttgcc acatagaccg tctctcctaa gcttaactct
     5641 aattcccctt ctaatacaaa aataaacgtt tcagataatg aaggttcaaa cgtcttatat
     5701 tctcctgcac cttcaaaagt cagcaagaca ggctccattt ctttttcatt cgaatcagcc
     5761 acgagccatt ccaattcata cccgttttct tcatcgaaat aaaccgtatg atcttctttt
     5821 gaataaacca cttgcagatt cgctgcttct tgatgaaaaa attcttctgg agtcacacct
     5881 aagacttcaa gaatagaaaa gaatgtttcc attgatggtg aacttaaatc tcgttccaac
     5941 tgggaaatat acccttttga caaatctgtg cgttcgccta gttcttcttg tgtcaaattc
     6001 ttttggatgc gcaaatttct taacttctca ccaatttcca taatttcctc cgaatcgaag
     6061 ttttctaata gtaaacttcc tgtttactac cactcgtatt agtatacgaa atttattgaa
     6121 aaaggcaacc ctttgtaaaa ttttttttgc tgaatatttc tttatctttt ggaataaatt
     6181 ctagttgttt gattctttat ttttcaccta atttttgtct aaatttacgg aagaacaaga
     6241 ataaaagagt aggctttatg gtatgataaa agaaaatttt aaaaggagtg tctcacgaat
     6301 gaacgctttt cgatcttttc aggtaataaa ggaacctgtt ttccatacag caatcagtga
     6361 tctgccattt actgagttgg ctgaaaatga agtcttggta aaaattgcct attctgatgt
     6421 caattataaa gatgcattgg caatgagcga gtctggtcag gtgatacgaa catatccgat
     6481 gactccaggg attgatttat ccggaactgt cgtccaaagt aacaatccac gtttttcaaa
     6541 agaagatctt gtacttgcta ctggttttgg gcttggcgtc acccatccag gcggttatag
     6601 ccaatatcaa aaagtaccag gagactggtt agttcctcta ccaaaaaata tgacgctgcg
     6661 tcaagcgatg atccttggta cagcagggtt tactgctatg ttgtgtgtga acgctttaat
     6721 ccgtcaaggt atgacctccg ataaaaaagt tgtcgttact ggtgcttctg gcggtgtagg
     6781 cagtacagcg atagccctgt tacataaact aggattccag tcaatcattg ccttttccag
     6841 aaaagaagag tctgtcacat ggctaaaaag tctaggtgct ttgcaagtcg tacgacctga
     6901 tgaatttctg ccagagacaa caaaagcttt aggaaaacag caaatcgact atgtcatcga
     6961 cacagtaggt ggcgagcagc taagcagtct tcttccattg atctcttata acggtgcagt
     7021 agctttatgt ggcaatgctg ggggaatcaa actaaacgcg actgtcttgc cttttattct
     7081 gcgaaacatc cagttgattg gaattgattc tgtcaatgta ccaatagatc aacgtctttc
     7141 tttatggcaa cagatggcgg atttgcagat tgcagatgaa ctggtcgtcc aagagattac
     7201 tctggatcaa ttgcctgaaa cagcatctaa actacttgct ggaacacacc aaggaagaac
     7261 actagtaaat gtaggtgacc ataaatgaag attctaatca cagctggagg aacgactgaa
     7321 aaaatcgatc aagtacgtgc tattacaaat cattcgacag gacgattagg gcaagcatta
     7381 gctgattatt tagctgccaa tccggatacc acaatcgatt acgtgactac gaaacaagca
     7441 ctgaaaccga aaagacactc caatataacg atttatacga tcgaatctgc actggactta
     7501 tttttacaat tagaagaact aacgaaaaaa gaacactacg atgcgattat ccacagtatg
     7561 gcagtcagtg attttactcc tgcttttagt ttttcagaag aacagctggc aaaaaaccta
     7621 cccacttctt caacgcaaga agaactggac aattggtttg cggaaaatga acagacagat
     7681 accactgttt caaagatctc ttctaacacc gagcatcttg tgctcgtttt gaagaaaaca
     7741 ccaaaaatca tttcttatct acgtgaatgg cagccaaaag caaaaatcat tggtttcaaa
     7801 ctattagtag atgtaccaaa ggaatctctt ctggcagtcg caaaaaatag tctgataaat
     7861 aacaaaactg attttatttt cgcaaatgat ttgacggaaa tccatggcga gacacaccac
     7921 ggctatctct tatctaaaga tggaacagtg gaagaagctc aaagtaaatc tgagattgct
     7981 gcattgatta ctgagaaaat tagactggag gaatcaaaat gaaaaagatc ttactaggcg
     8041 ttactggaag tatttctgct tacaaaagcg ctgatcttac aagtcaattc gtcaaattag
     8101 gtgcccaggt agatgtgatt atgtctaaaa gcagcacaca ttttatcacg ccattaacct
     8161 tgcaatctct gtcgaagcgc gcagtccata cagacgtcat gcaagaatca aatccgcagg
     8221 taatcaatca tatcgaacta gcaaaacaag ctgacttgtt tctcatcgcg cctgctacag
     8281 caaacattat cggcaagcta gcaaatggca ttgcagacga tcttctttca acagtcgcaa
     8341 tggcgcttcc agtgggaaca ccaaaattga ttgcccctgc catgaacacg aatatgtatc
     8401 aaaacacgat cactcagcgt aatctggaaa cattgagatc tgtcggctat caggaaattg
     8461 aaccaagaga atctctttta gcttgtggag attttggaaa aggtgcctta gctgatattc
     8521 agacgatcat cgaacaggtc atgctgactc ttaatgaaag aaatgcttag aaaaaaggag
     8581 tacttatgaa aaatacaaaa aactttacat taactgcgat gtttttagct attttgattt
     8641 tattagctgt tacgccttta ggttttatcc ctatcggtcc aatcaatgca acaaccatgc
     8701 atattcctgt aatcattgct tcgatcgttt taggaccaag actaggtgct tttcttggcg
     8761 gtacatttgg actgatcagc atgatccgta gcacagttat ccaaacacct ttgtcttttg
     8821 tcttttctcc gtttatccca gtcatcggaa cagaacatgg aagctggaaa gccttgctga
     8881 tcgcgtttat tcctcgcatc ttgattgggg tggttcctta tttcgtttat aaaggtctgc
     8941 gcaaactaat gaaacaaaaa gcagattccg tttctttatt cttagctgga ctttccggtt
     9001 cattgaccaa tacgatttta gtgatgaata tgatctactt tttattccag caagattatg
     9061 ccaaagtcat cggaacaaat ttaaatgctg tttattcagc tattctagcg gttattttta
     9121 caagtggtat acctgaagga atcgtcgcag gtcttgcgac tgcagcagta tgcaatgtct
     9181 tgttaagata cttcaagcac ccttctctta ctaactgata gtcatcgtag ccgatctctg
     9241 atttttttct cttttggtgt tactattgaa tggaaagatt tgacgtcgaa aggatgatcc
     9301 aaatgaaaat ttttgttgta ggtgcaaacg gacaaattgg ccgacacctg ataaaagatt
     9361 tagctccaag ttcccatgaa atttttgcag gggtgagaga tgttgcttcc caaacattag
     9421 taaaaaaaga gaatgtttcg tatgtctctt ttgatttaac ttggtccgtg gaaaaaatga
     9481 ccgaagcatt caaaggcatc gatgtattga tttttgctgc cggctctcaa ggaaagaatc
     9541 ttctgcaagt agacttggat ggcgcaatca aaacagtgat cgcagctgaa aatgcgcatg
     9601 tctctcgcta tctgatggtc agtgctgttt atgcagatga accagcaaaa tggcctgagt
     9661 ccatgacaga ttattatatt actaaacatt atgcagatga atggctgaaa cgaactaact
     9721 tagattttgt cattcttcaa ccagtcacat tgactaacga tgaagaagtt acttccattc
     9781 aattaactaa gcctaatgag aaggcatcaa aaacgatcac tcgctctaca gttgctgctg
     9841 tacttgccgc tctagtggaa gaaacggata tttctcgtac gactctagta ctttctgaag
     9901 gtagcaaaga gttgaataca gcattccaag aatgggcaaa ggaggaatag atccatgcgt
     9961 gccatttcaa tcaagcatcc tggttctgta gatgtattag aacaaattga agtgcctgtt
    10021 cctaaaccaa aacaaggcga attactgatc aaagtccata cagcagctgt caatcgaaca
    10081 gatattatga aaagagaatc agtctctttg aaacctccct accctatttt gggagtcgaa
    10141 gtagctggag aggtagttga gaataaaagt gatatctcca cttttactcc tgggacaaaa
    10201 gtatacggat tagtcaatct aggtggttat gccgaatatg ctgtgatgcc tgccgatcgt
    10261 gcaatccttc tccctgatac gctagactat gtttctgctg ctggaatctc agaagttttt
    10321 ttgactgctt atcaaacact ttactggctt ggaaaattac aagaaggcga aactgtcttg
    10381 atccatgcag gagcaagtgg tgtgggaaca gcagcgatcc aattagctag acagctttct
    10441 cacgcgaaaa tcatcgttac tgcaggatca gaagaaaaac tcgccttttg taaagaactt
    10501 ggtgcagatg aattgatcaa ctataaaaaa caagactttc aagaagaggt acgaaaaatc
    10561 actgaaggtc ggggagtcga tgttattttg gatttcatcg gcgcttctta ctgggaaaag
    10621 aatttagctt ctatagccgt tgatggacgt tgggtcttga ttggtatgct aggtgggacc
    10681 atcgtgcctg agatagacct tggtgtattg attgggaaac gtgtccaact gcttggtaca
    10741 ttattgacac ctagaagtga tcagtataaa gcagagctcg cacaaacttt tatgcaaaaa
    10801 gctgggcctt ttctagaaaa tggcaagatt cggccaatca ttgaccgtac attccaattc
    10861 agcgcagtca aacaagcgca tgaatatatg gaagcaaaca aaaacatcgg aaaaatcatt
    10921 ttgaagataa ctgaataaaa tcatgctgat caaaaaagag gctgggacag aagcgtttaa
    10981 ctccaagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcagct
    11041 tatttccgaa ggaatttctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa
    11101 agtgttcttt acttttgtcc cacgctcttt ttccgaataa acggtgttca aaagcttgca
    11161 tctgcggtaa taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc
    11221 ttattacttg atgcagggca gctttttcac aacctcttca ctaaacagtg ttcaatcagc
    11281 tgaccctcgg tattagctca aaacgctgat ttcacaacct ctttttattt acgttctatt
    11341 tcattcattt tccgaagcaa atcgagctgc ttgtatgccc gcttgtcgcc caaaaataat
    11401 gatctccgca actgaatttc caccgatccg gttgtttcca tgtaagccac ctgtgacttc
    11461 accagcagca tataaccccc taattggctg gttttcttta tctaatactt cagtgttcgt
    11521 attgattttc acaccgccca tcgtgtaatg gattcctggt gcgattttga tcgcataata
    11581 attcggagac gataagtcat gttccatggc tgtggcacgg ttgaattgag catctttttt
    11641 ctccgcaaca tccgtattcc atccatcaac tgtttttgct agtgtttctt ttggtaaatc
    11701 gattttttct gctaattctt ccattgtctt tccagttgtg acatatcctt tttggtcata
    11761 aaattcaata gctgtggcac gatcacgcac gccttggtca aaaatcaaat aagctgattt
    11821 ttcaggcaat gctgtaattg ctgcagatac tttatccctt gtatccattt cattgacaaa
    11881 tcttttcccc tcttgatcga ccaagattgc tccttctccg cgaacaacct caccaatcaa
    11941 gaccccctca tcctgctgca cagttggatg aatttgtatt ttatccatat caacggtttg
    12001 tccacctaat ttttcaatca tggtgatacc atctccagtt gatccttctt gatttgtcgt
    12061 aatataatca gctaaatcgg gacgatattt ttcaataaat tctttgctgg caccaaaacc
    12121 gcctgtggta actacgattg cttttccggt gacctctttt ggttcttctc cttgaacatg
    12181 tacagtcact tcattgaccg tttccccatt tttcttaatg tctgtcacat ctgcgttgac
    12241 aaaaataggg atttcccgct catggacgtt attcaataac ccctccacta agtatccacc
    12301 aatagccgag ccatcagacg gacgatgtgt cctcttttca ctcatccctc cagtgatcgt
    12361 taagttgtcc agtttgatac cgttttgatc tagccaatcg accgcatcag cagagtgatc
    12421 gacaaagtat cgaagcattt ctttatcatt cgttcctcca ccacctttta gcgtttcttc
    12481 atagaaggca tcattcgagt ctttgattcc ttcttctttt tgaaattttg tttctgaagc
    12541 attcataccg cttgatgatt tagatgtgtt tcctccggca acaggcatct tttccaaaat
    12601 tactggattc aacccagcat cttttgcttc gatagctgct gccatccctg caccaccagc
    12661 tccaataatc acgacatcat aagattcttt catttctttt ggatctgtat atacttgttc
    12721 agaagcgcct gatgatactt cggcagattc cttcgtacta gaacttgtct gcgctgtatt
    12781 tttctgatct gcgccgcatg cgctcatagc aagaagtgca acggctgtta ataaaccagc
    12841 aactaatttt ttcatttgta tccccctttt ctccttatac tatattaagt gatttttttc
    12901 acaatatcaa ctctgttttt ctttaagtca tatagctttg gtaaacacac gcttcttagt
    12961 ataattaagg atatacatga aggagttatt ttatgaatca tttgaaaacc ttttttacga
    13021 acagtacaaa aactgcaggt agaatcacaa acggaatcta tattttatta cgtatcatcg
    13081 tcgttattat gctagtggat caggtctgga accaacactg gggaaataca atgcttttat
    13141 tactgacatt aattttattt gaaatccctt attttctgga aaagatatta aagatacaga
    13201 ttccaaatat gctagaaatc attttgttat cttttatttt tagttcaaca gttttaggag
    13261 agttaagtga tttttatggc tatttttcaa tgtgggatac ttttctacat gcattcagcg
    13321 gatttttagc tgggggtgtc ggcttttccc ttgtttattt gctcaataaa aatacaaaaa
    13381 ccatgaactt gactccttta ttactagcag ttgtttcctt ctgtttttcg atgacagttg
    13441 gtgttatgtg ggagtttgtg gaattttcag cagataaatt actgcaattc gatatgcaaa
    13501 aagatacgat catccagcaa atcaatagtg tggagttaag taaagacgga aacactgtct
    13561 atcatgttcc agatatcact cggacaatca ttgaaagtca aaatgcagat ggtaaaacag
    13621 ataaaacaat catccaaaat ggttacttag acgtgggaat catggatact atgaaggatt
    13681 tatttgttaa ttttattgga gcagttgttt tcagtgtatt tggttacctc tatagtcgaa
    13741 acgattacca aaaatttacg ttcatcaaga attttattcc taaaaaaatc aaacgataaa
    13801 acatgctaaa aaacaagaga aggggcagtg acaatacttt tgtcactgcc ccttcttttt
    13861 tgaatgtatg gcatgccatt agttatccga tagtattaac tcaaaaaagt aagatatgag
    13921 gaatgactat ttcgtttttt atcccaatac tgaggactta tttcaccatt tcgttattta
    13981 tctaatttac ttaaaacctg cataactttt tcataatctg gattaggtgt gccgtctaga
    14041 ttttcagcaa taaaagtaaa acccattttt ttataaagag caatcgcctg atggctccat
    14101 gtctgtgtgt gaagatagat tggtccgtcc gtatggaggg taggaaattc ttgcaagact
    14161 ctggcagtca atgcttttgc gagccctttt ccttggtaat ctggtttcac tgctaaccaa
    14221 tgaaaaaccg gatacacttg ctctcttgat ttttttcgcc aagcggtaca ggttgctact
    14281 ttttctcctt tttttgtctc tacaaaaaac atccttcttt caagttcttc aggataagga
    14341 gcaaagtttt gttgaaaata ctcaagtgct tcatctgtcg tttgaaattc cccaacggaa
    14401 gtctcaatct ccgcccaagc agattcatct tctttttgat aaaatcgaaa ttcaaaacct
    14461 tccgctaaag gcgtgattgg aacattttgt tgaagtggtc ttgtcatcca gatttctgcg
    14521 taaggtaacg ttttatctaa catttttcta tccctcctcg tctccattat gccaaataac
    14581 atatgactca gctacttatt tgactataac agatagagat ataaatcaga accagtcatc
    14641 tgcgtcaaat tcatttcgat tagcgtatac ccatgttcct gccatacctc gcatgaaatc
    14701 tcctaaagca gaagaaggaa tacctccacc aggtgcgggt gttcccggac taggaagtgg
    14761 tacttgcggc tgcagatgct catcggtgga atcaatagtt gacaagcgtt ttttcttcgt
    14821 ttccttctgg tcatctgatt ccttctgatc ttccgactgt ttagcaacaa attctttttc
    14881 tgattgttgt tttattttag gctctaaaat ttcattttcc aatccagaaa cttgtggggc
    14941 caaatgagct tcttgcctta tcgttttatt agattctgag atttttctag atattcttgc
    15001 taactctagg cttcttgttt gttttacttt ccctgttgga gaaactgcat gagccatttg
    15061 ttgttttttt tctgacaagc tttcttcttg tcttttttgt tctttttgat ctatctgttt
    15121 gtttaatggt aaaacatgta atagtgactg agcatggagt tcttggagtg aatacgggtc
    15181 atgataacta tacataatcg taaaatcacc tgtttgtttg gtcaaataac catcctccaa
    15241 tggtacttca ttgattgtaa ccgatacatc ctttaacggt acgggtttac ctaaggaatt
    15301 tgttgctgat tggatcaact cttctttgtt tattttttcg tttggatgaa caataagatc
    15361 atgaccttct aatgaacgat tgtctggtat ataaacataa gtaaaggtaa tttcccctcc
    15421 tgattgcttc ctattattca tcagttctga gtgaagtact tcaaggatct ccgaccagtg
    15481 actttttctg tctaatccgt ttgcttgcat ccaatcagaa atagaagtga ctttcccatt
    15541 ttgttcataa gtcgataaat ctatatttgg cactcgatca gaaaattcag caggtaggcg
    15601 caaatcagga atacgattta cactactcat atgtgtttct gcttgaagtt ctttatttgt
    15661 ttgactatct ataaaatgag tgatgacagt cgatgaagca gcagatgctt ctacataaac
    15721 tacagctgtt gtttctgcca catttgatgt tttcgagtct atataagtta atacaacggg
    15781 atattctccc ggagttgtag tatctactgt cccgttcatt ttgatttctt ctttatttcc
    15841 tgaagttcca tcgctttttg ttacatattc aacaagatct ttaggggcgt aactctctcc
    15901 tgttcttaag tgaagatctt ttcctttgat agctgtttga tcagcacgaa catggatcac
    15961 agcagatgca gtcattgttt ttaaagcaat tggatcaaaa taggacaagg aaatagtaga
    16021 tgttcctgta ttagtcgtat ccacgatatt cccattgttt gaaattactt tggacatatc
    16081 tccttgtgtt ccatctgcgt tcttcagcct ttctaccaga tcagatatcg aaacttttgt
    16141 ccctgccaga acatcgattt tctttctttc aaactgtgaa taatcttttt catatacata
    16201 agaaacttgt gcgcttccac cgtcctttat atctgtcaaa gcatgattca aaatatcagt
    16261 gatctgtttt aactgatcac cttttgtagg atcagtcata tcggaaaaga tagaaagcgg
    16321 cacactgctt ccggtttccg atgtccataa tgttttgtta ggaattaacc agtagtggtt
    16381 tatcgttgga ggataactga ctttattata ggaagtcaat gcaggaagta gctgttttcc
    16441 atttgcatca gtgaaagtat gagtaatagt gataggggaa tcagtggtct cagaaacaga
    16501 ggtacgtacg accttgatgg tttgtgtcgt tgcctgaacg ggtacatgca tatagacacc
    16561 tacagataaa ctgatcagca tcgtgcgcat cataaactta acgtagtgtt tttcagaaca
    16621 cgaccgttcc ttatctaaac gacaagcgtg tttatttagt tgttttgaac gtctttttga
    16681 acacatttat tcttagtcct ccctactctt gtctaaacat gaagtagtaa ttttcataag
    16741 atgatttcat tatataaata aaatcaaaag aagatggact attaggagag tatttttttt
    16801 gattttttat tgtttcaaga aaatcggaat aataacttat gtacttttta attttgttaa
    16861 gtaatatatc tagttatatc tagttatatc tagttacctt ttctttgaaa aaaacataaa
    16921 tcagctaata aatatgtgag acaaagctgc catccataaa tactagctga ttttttcagt
    16981 taaatgtcta tttttttctc tgatgatggt atttccaaaa tattcccagt catctggagt
    17041 cggtggattt ttccaaatta tctgttcttt attaaatttt tcaagtaaac aatcagataa
    17101 ataaatattc gtacgagaag tgattcgact ctcaatcaca atattgtaat ttttaaatcc
    17161 tttcacttga gaaataatgt aatcggtata agcagatcct tgcgaaaaat caatacagac
    17221 atgaatcggt gcttcaaaat atgataccgg acaaataccg accaataaaa agagataatc
    17281 ataaaataag cgaatcatga aacgttcatc ttttcccaag aaatagcgtt tattctcttg
    17341 tatgaccttt tggattgctg tactgaatat tggatacgtc tcgaagaaaa actgtagctg
    17401 ctttttagac gtaaagctag tcatctcaaa gtcgaatatc caattttttc gattgacatg
    17461 catcataccc atacgaagtt cttttcgtat atatagtctt tcttcttcag agatttcaat
    17521 cgaaaatggt aattgatcaa tcaattccac tgacattttt tgactaatca tgtggatatc
    17581 atgaaaatga gcttccgcaa gtccaaatgc gccatctaat tcaaattctt cttccgtgat
    17641 caaaaacaac atgatataag aaatctcatc aatgatctgc tctttaggga tatgaagaaa
    17701 agaaatatgt gataaatcct tgatgatctt cattccttct gagctcttaa agtattcatc
    17761 tgaaaagaaa cgttttccga ttggttgatt cccataggcc ctcaacaaac taatagtggt
    17821 tagcaaattg aatttgattc gttttgttgc agacagtttt aaattgaaca gatattcaaa
    17881 atattgcacg atactctttg cttcattcat gacgctttct ctaaatggca tagaaaaacc
    17941 attaaagacc ccgaaatata ccccaaataa tacgttccgt atgtttattt cttccccaac
    18001 taatatgttt ttctttaact ttatttgatg ctgttcaaga aaagagacta attctcttct
    18061 ttttacatag gctcttgata tgcttaaaaa atgattctct gcaaattttt cgatcgttac
    18121 ttttgaatcc acgatctccg ttaacagtcg agcaattttt gatcgttgaa agtagattct
    18181 tcttaaattt ttgatatccg tcaaattcag acgttcgaca tggatctctg cttcttctga
    18241 aatttggatc tctggctttg ttttgaattc agtaaattct ttatttaatt ctatgatata
    18301 atttcttgtt ttaaatttag acaaacctaa tgtttcatat aacttaggca gcactacata
    18361 attctttttc tcaaattcta aatatctagc tagcagcagt ttttctctat cttcctcttc
    18421 tataaggtaa tccaacttca tatcctacac ctcaaaaaat gcctcatacc aattacttga
    18481 tatgcagatg aaagttcatc ttttcaccta tactgatttt atgatggatt atgcttgttt
    18541 tttcatcagt tattctcatt ttttatcttt tttttgagaa tattgcaaaa aatggaaaaa
    18601 tttatgagga aatattctat attttttgtt ttattgtgtt aaaatgtaca agatttgcgc
    18661 catttgcgta aacaaccaca aaaaagagca ggacggccac cgtcctgctc ttttacttat
    18721 tttaagttcc attacttatt aattggctaa acttctgctt gtttctgata ttgcttgttt
    18781 ttctccaatg atccgcactt ctgtttctaa agacacatca aatttttctt tgattacttc
    18841 ttggatgtgc gcaatcaatt caatataatc tgtcgctgtc gcatgatcga tattgacgat
    18901 gaatccagca tgtttttcag agacttgtgc accgccccat ttcaggcctt gcaatcctgc
    18961 ttgttggatc agctgacctg tatagtgtcc tactgggcgc ttaaagacac ttccgcatga
    19021 agggtattct aatggctgtt ttgattgacg caattcggtc aactcatcca ttcttgcttt
    19081 gattgcttca taatcccctg tttgcaaact gaatcgagct tccaatacga tagcacgtaa
    19141 ttcttggact ttactgtgac gataagaaaa taccatttcg tcttttgtca aagttttcaa
    19201 tgttccatct tcaagcaaaa gatccacttc agcaaaaaca tctttgatct ctccgccata
    19261 cgcacctgca ttcatataaa ccgctccacc gacacttccc gggatgccgc aagcaaattc
    19321 aaatccagtc aatgattctg ccaaagctgc ataagtcgta tcaatcagtg aggcacctgc
    19381 ttccgcaatg accatcgttc ctttgacatg aatctgtttc atttcagtca gcatgatgac
    19441 tacaccgcga atcccgccat cttgaacgat cagattgctt gcatttccta ataccatcca
    19501 aggtatatca tggattcggc aataatcgat gatttgcttt acttcttctt gttttttcgg
    19561 gaatgcaagg acatcagctg gtccacctgt tttcgtaaac gtatagttca tcaatggttc
    19621 atcaaacaat atagtaatcg tggctaagct ttcaactatt tcttctttat tcactgagga
    19681 ttttcctttc ttttctcaaa cgtcctcatt attctatcac gcttaataca atcttgccag
    19741 tcttgttcaa agttgatgac ggtagtgatc tttaagcact tcctcacata attcatgact
    19801 tcttacaact tctttttgtt taagcaattc tgtttccgat ttacctaaac ggatctgaga
    19861 aaagaaggcc tctaccattg gctcaaaccc gcgtttgact agcgtgtttg tccagtcgcc
    19921 aaattctttc actttttttc catcagcatc ttgaatgaca agctgggtca gatcgtttac
    19981 ttcataagta ccatttttac ttgttacttg atatttttct atattagcgc cacttgctaa
    20041 atccatcgtt aaaatagccg tttgattttg tgtttctaat tgtaaaatgg catattccaa
    20101 aaattccttt gtttctctta tctggtattt tgttctcaaa atgggtttat ctaataaata
    20161 cacagctgta tcaataagat gtaaaaacaa atcataaagg acgaactctg tcgtttcttt
    20221 tgccgctatt cgattttttt gtaggtggat caaccgctta tctgggatac gtttcaattc
    20281 ttctaccata ggagcaaagc gccggttgaa accgatcatc aaaattattt ggttcttatc
    20341 agctaattct tgcaattcac gaacttcatt tagatggaca ctcagtggtt tgtcaatata
    20401 aacatggata ccattttcta gacatttttt ggctaattca taatgtactt ttgttgctgc
    20461 gtggatcata catgcttcga ttttttcttc taaaagctca tcgatcgttt catatagatg
    20521 ttggaacccg tactcttttt gaattctttc ttttgtttct ttatttcttg ttgcaaaata
    20581 aaaatcaagt gttccttgtt ttttcgcata agttggtaaa tatgcttttt ctgcgatatt
    20641 ccctacaccg attacaccaa tcttcatcta ctttcctcct ggatcattta gttactttca
    20701 tcttacacca ttttttacta aaatgaggta caatagtatc gcaaaggtaa ggaaattaat
    20761 tcgaaggagt gtgcttttat tgaaatttat ctcttggaac gtaaacgggc ttcgtgccat
    20821 cgtaaataaa aattttttag aagtttttca tgagttagac gccgattttt tctgtctgca
    20881 ggaaacgaaa ctccaagcag gacaaattga tttagatctt ccaggttatt atcaatattg
    20941 gaattacgcg gaacgaaaag gctattccgg cacagcgatt tttgctaaaa aaccagcatt
    21001 gaatgccact tacggaatgg gtatcgatat ccatgataca gaaggccgtc tgattacttt
    21061 ggaatattcc gatttctttc ttgtaacctg ttacacgcca aattctcaaa gcgaattaaa
    21121 acgtttagat taccgattag aatgggaaga agccttttat aattatttgg aaaatctaaa
    21181 aaaacaaaaa ccagtcatcg tgtgtgggga tctaaatgtc gctcatcaaa agattgattt
    21241 gaaaaactgg aaaacgaatc aaaaaaatgc tggattcact ccagaagaaa gagcggctct
    21301 ttctcgactg ttggataacg ggtttatcga tacattccgt tatttttatc caacccagga
    21361 aggtgtctac tcttggtgga actaccgatt caattcaagg aaaaacaatg ctggttggcg
    21421 gatcgattac tttttgacga gcaaagattt agagccagga ctagctgatg caaagatcca
    21481 tacggacatt atgggcagtg atcattgtcc agtagaatta gatttgaaat aaagaggttg
    21541 tgaaaaagtc gtttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt
    21601 ttgcataaat ttggcataat accgagaaac tggcttttga acactgttta tcgagagatt
    21661 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
    21721 ttttcgtttg gttgggctta ttaccgcaga tacaagcttt tgaacaccgt ttattaagag
    21781 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    21841 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttattaa
    21901 gaggttgtga aaaagctgca ctgcatcaag taataagaac agccaaacaa aaatagttcc
    21961 tcatattttt cgtttggttg gacttattac cgcagatgca agcttttgaa caccgtttat
    22021 tgagaagttg tgaaatcagc gtcctgacct gagtattaga gaaacaaatg gcaaaatagt
    22081 tcctcatatt ttgctatttt ttgagctaat accgagggtc agctgattga acaccgttta
    22141 gtggagaagt tgtgaaaaag tcgcctactt tcgagtcgta agaaattttt ttgaaaatgg
    22201 ttcctcacaa ctttgcataa atttgactta ataccgagaa gtcatgatcc ccatctaact
    22261 ccaaatcaca aggaatgagt tcaaaaacta gcttttctca acaaatatta cataaaaaat
    22321 tctatgtttt ttgttaaatt tatttgcttt tttcaaaagt atgttacagt aatttcccaa
    22381 ggaggtttta cagaaatgaa ttttgttgcc atggattttg aaactgccaa tcatcagcct
    22441 tatagtgctt gttctcttgc gctagtcatg gtaaaaaata gccagatcgt tgatgaattc
    22501 tatacattga tacagcctga gactcctttt ttctggcgaa atgtccaaat tcacggaatc
    22561 catcaagaag acgtacgcaa tgcgccgaaa ttcccagaag tctggcaaac tatccaacca
    22621 tattttcaaa aaaatcgttt agtcgttgca cataatgctg cttttgatac aaaggtctta
    22681 gcgggatgct tagattatta tcatcttgaa cagcctaatt atctttcttt atgtacagtg
    22741 aaaagtagcc gtcgcctgtt tcctgaattt ccaaatcatc gattgaacac tgtgtgcgaa
    22801 aatttgaaca tccaattaga tcatcaccat gatgcattgg aagatagtcg tgcatgtgcg
    22861 gaaatcctgc tttatcaaga aaaaaaattc gggacagatc cattgaaaaa acttgtgact
    22921 ataaagtaaa agaatactta tgaaacacac aaagaggtcg tgagcctgcc gtttcactcc
    22981 gagtcacaag gagcaatttc aaaaaatagc ttctcatgtt tattaaatat tgtgactggt
    23041 gaccgaggag ctggcaggag aacaccgttt attggagagg ttgtgaaaaa gctgttctgc
    23101 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttggact
    23161 tattaccgca gatgcaagct tttggacacc gtttaccaag aggttgtgaa atcagcgttt
    23221 tgacctgagt attagagaaa caaacggcaa aatagttcct catattttgc cgttttttga
    23281 gctaataccg aaggtcagct gattgaacac cgtttattca aaaaaaatgg actgcgacac
    23341 gacttttgtc acagtccctt cgtgtgtttt tctgccttct caaaaggatg acagtttctc
    23401 actcttttcc tatttcttct attcaatcaa ataactcatt ttatatacat ctaaaaattc
    23461 acctagatct gttcttgccc ctctaggcat gaccgcttcg atttggaagc ccatttttcg
    23521 gtaaaggtgt acggctcttt cattacgaac ttgaacgtca agctccaatc gaaaaagtac
    23581 acccatttcc ttcgcccaac tgatgatttc ttctagcata agagtgccta gtcccattcc
    23641 ccaatattcc tgcagaatac tgataccgac ttctcccacg tgagacaaac gaaattgact
    23701 gtctgccttg acagatgctg ttccaataat cgtcccttca tagattgcaa gcagcaataa
    23761 attattattg ctttcgtata tataatccaa ctcttcttct aaggtttctg gtggtaacag
    23821 gagttctgct tcatccaaaa ctaaaaattc tgtctcctta ttaacttgtc ccatcatcaa
    23881 aagaatgtct tttgcatctc ctggcactgc ttcccgcaag atgatcccaa cttcttcact
    23941 catgattttc agcccatttt tctagaattc gctgtttgaa ggtttcactt tgctccccgt
    24001 atgagattag tttcacatac cgcttttcta aatctgtatc acttttttct aaaatcagtt
    24061 ccaaataatc ctccggcagt gcttcttcta atagatttcc ccattcaaca acggaaagcc
    24121 catcaccttc aaaatattca tctaacccta aatctgctcc gctagctgct acccgataaa
    24181 tatccatatg atacaacgga atccgacctt gactatattc tctaataatc gtgtatgtcg
    24241 gacttttgat catttgcgtt atccctaatc cttgggcaat tcctttcgtc aacgttgttt
    24301 tacccgcacc tagatcacca gtcaatatca gattgtcacc tggcattgcg gaatgaccaa
    24361 ttgcctctgc gaattgttct gtcattgtta atcctgatag ttcgatcatt tttcatcttc
    24421 tcccgctctt ctttcttttt gccagtatat cgcgtaatga agttttttta aagaaaaaat
    24481 gaactagtca agaacagcag ctgctgtttt tggctagttc gcgtgtattt gttaatcaat
    24541 caatgtttgt gctgctgtga taatcgataa tttatataca tcttcttcat ttgctccacg
    24601 agacaaatca gaaaccggtt tgttcaagcc ttgtagaatt ggtccgattg cttcaaagtt
    24661 accgaaacgc tgagcaattt tatagccgat atttcctgac tgcaattctg gaaatacaaa
    24721 gactgttgct tgaccagcta cttctgaatc tggcgctttc agttgtgcaa ctgaaggaac
    24781 ataagaagca tcaaattgca attcgccgtc gatcatgtat tgtggcgcta gttttttagc
    24841 gattttagtt gcttcaacta ctttgtctac ttctggcgat ttcgctgagc cttttgtcga
    24901 gaagctcatc attgctactt ttggttcgat atcgaacaat tctgctgttt ttgcagaatc
    24961 tacggcgatt tcagccaatt cttgcgcagt tggattcacg ttgatcgcac aatccgaaaa
    25021 gacatatttt tcttgatctc ttccacgtac catgatcatc gctccgcttg tccgactgat
    25081 gccaggcttt gttttgatga tctgaagtgc tgggcgaaca gtgtcgccag tcgaatggat
    25141 cgctccagag accattccgt ctgcaatccc catatacgta agcatcgtac caaagtaatt
    25201 cacatctttt aaaatctttt ctgcttgttc tttggatgcc tttccatttc tacgttcaac
    25261 aaaagtatcc accatttcat cccacttatc gtaattatca gggctgatga tcgtaaagct
    25321 agatgttttg attcctctgg catgcgctgc atttcttatt tcttcttcgt tccctactaa
    25381 aatgggttcc acaagttctt ctgctttcaa ccgggcagct gcgcctaaaa tacgagcatc
    25441 agtagcttcc gggaaaacga ttttgatccc acggcgaaca attttaaatc ttaaactatc
    25501 aaacaattcc acaagatgac ctccagacgt ttttatatgt attatcatac actattccag
    25561 aaaaaaagaa cagtttattt tatctgtatc agtcaaaaat gctggaaaaa ttaagttttt
    25621 cacatacaaa gcgaactgca ctataacagg aaagcgctgt tttattacac tgtttcaggc
    25681 agttgccaat caatcggttc ttctcctaac gcaaggagcg catcattcgt tttcgagaag
    25741 ggtttggaac caaaaaagcc acgacttgca gataacggac tgggatgtgg agatgttatg
    25801 atgatatggc gactcttatc gatcattttg attttttctt gtgccggttt tccccacaag
    25861 ataaacacga ttggtttttc ccgttcattt aatttttcaa tgatcgtgtc tgttaatctt
    25921 tcccaacctt tcccgcgatg agaatacgct tgcccttccc gaacggtaag aacggtgttg
    25981 agtaaaagga ctccctgttt agcccatgac actagatttc catgttgaac aggtgcgatt
    26041 ccaagatcat cataaagctc tttatagata tttcttaacg atggcgggat ctttacgcca
    26101 ggttgcacag aaaaagataa gccatgagct tgattttctc catgataagg atcttgtcct
    26161 aaaatcacca ctttaacttc ttcataagga gtcaattcca atgcttcata gatatgatac
    26221 atatctggat ggatcttttg ggacgcatat tcttttttta aaaattctct tagatgcaga
    26281 taataatctt ttgaaaattc tgaggacaaa acttcttgcc aactgttatg aatgatttct
    26341 ttcaaaaact ccacctccta ataaaaagaa taactcacat tttcttcaat cacaagggac
    26401 aatcttcacc ctcagcactt gattaatatg gtaaactagg aacaagaagc agaaaggaac
    26461 gataatcatt atgatcaaat taattgcatc agatatggac ggcacattat tagatgccca
    26521 tatgagtgtt tctcctgaaa atgccgaggc gatacgtttt gcaaatgatg ccggtgttga
    26581 gttcatggtc gctactggtc gaaactatca agaagctcgc gcagctttag atgaggtagg
    26641 aatcgattgt gcgatgatca ctttgaacgg ggcacaggta tttgataaag aaggaaattc
    26701 attgtttacc gtgtcgattc caaataatca agcaatctct gttttagata ttctagatgc
    26761 taacggtatt tactatgaag tagcaaccaa cgatggtctt tattctgaaa atcaagcgaa
    26821 acgaattgaa agttttgctt ccatggtggc tacccattta cctcacctta cgtataaaat
    26881 ggcgattgcc atggcctcag ctaagctgga gttattgcac atcacatacg tggacagtat
    26941 tcgtgctatt ttggaagaaa agaatctgga agttttaaaa attatttgtt ttcatacaga
    27001 agggccagct gtattaggtc cagttggaaa gcagatttct cttttaggcg atctagccgt
    27061 tacttcctct ggtcaaaaca atatcgaagt gaaccataaa aacgctcaaa aaggaattgc
    27121 tgttgcccat gtcgctcacg agcgtgggat ttctttagaa gaagtcatga cgattggcga
    27181 taacttcaat gatgtcagca tgctgcaagt agcaggagtc agtttcgcaa tgggaaatgc
    27241 agaaatcgaa gtaaaagatt atgccaaata tacaacggac acaaaccttg aatctggcgt
    27301 aggaaaagcc atattgcgcg cgattgcaga agacttgtag ttttatctag aaaagaggga
    27361 tatagatgtc agaatttttc attcaggaaa agcagcttgg acgtgcaact cggacaattg
    27421 tcaaaaacga aaaaggacat tccctgtttt tgatggttgg tcgctgggga acaaagggcg
    27481 atgcattgtc tctttatgcc atgaatggcg atctcgttgc tcacatcaag cagataagcc
    27541 tgacattcgg tacgcgtttt gagctgtata aagagttcga aaaggtggga accatgcaga
    27601 aaatattcaa ttggcccggt gacttttatt atattcgtag acttcactgg acagtccaag
    27661 gtgatatcta taatcatcaa tatcaaatcc atcatttcaa tcagcaaatc atgaaaatgg
    27721 ataaagcaac acttttttca ggagattact atgtattgga tgtctcaaat gatgaagatg
    27781 ctccagtttg tatttgtatt gctgcggttt tagattactg gctctaccgt aaagacaaaa
    27841 gtcgcgataa gcctgtttct taccgactta acttaagtta aaaagatgta cctgaaatgc
    27901 ggaaatacgc atttcaggta catcttttta gatattcttt tgcatatgat cataaagatg
    27961 accactgatc atccgctgac cgacaacttt gaagtcatgg cgttcgtata attttttagc
    28021 ctttggattt ttttcatcca cacttagacc gatgacggag cgattagctt tcttcgccaa
    28081 tttaggtaat gcttctaata atcgcgaacc gatgccttgt cctctaaaat cttctgatac
    28141 actgatagag tccaaatacc attcgttggg aaacgtttct ttatcaatga acaaacgtgt
    28201 agacgcatct agctgatatt tctctaagat aggtaccagt ggctgatcga tgattgattc
    28261 ttcttcatct gtataaccgt aagcaactcc tgcaacttta ccgtctattt cttctacgat
    28321 acctcgtgaa taactgtaac gataatttgg atcagtgatt gcttcttcta aaatctctaa
    28381 agtcttttgt tcccctgtct gcttcaaaaa tggcagttcc atatctttta gaatcactaa
    28441 gatcaatggg gcgatttctg gagcatcttc ttttctcgca aaacgaatca tagtttcctc
    28501 tccttttctc cattctaaca tgcagaaaaa gaaatcgcaa aatcgttatt ttaaaaaatc
    28561 ttcaatcttt tcagtaatga aataaccatg agtaacgatt cttgccgctt cagttcggca
    28621 gtaagggcag acgactggca tttctttatc attatgttcg actacattat tgacttcttc
    28681 gtatgcaaat ttttttccgc aattagggca tttcattttt tccatgttat ctcaacttcc
    28741 tttcatcttt actctcttag cttactctta tattgtaaaa aagagaagca atatccttct
    28801 tctatgcaga tatccatacc tgacggtcct gttctccatc tattaatcgg actttataat
    28861 aaggacgttc atctttgact agaagtgtcc actctttcac ttttgctttt ttcccgtagt
    28921 agctgactgc ttccttcatc gctgcttgcg gttgtttgaa ctcttccgtt ttttctatat
    28981 caaaaagatg ttctgcggcc ttttccgtgt catttcgtac ggttgttttc ccagtggatg
    29041 gattgactgc tatgacttgt ccttcacaaa aaaactggta ttcatatgtt gcattcgtag
    29101 gattctccaa atctttttca actgcaaaat gaatagcaga tatgttatgt tcttttgttt
    29161 cgttttttac gattgctgct gcttttttca atgagatccg atattcctgg tcactaatat
    29221 catccatgtg aaaagcggca ttgagcagtc ccgtcatacc ggtaatccct attacgatga
    29281 gggcagcaac accaaacatc atttttttca ttcttcattc tcctgtttct tttttttaaa
    29341 gaatatcaag aaatgaacga atgaaaaatc gaatgaaacg aaaaaaaagt gacaattttg
    29401 ttatctttat tcgtgcagtt ctaatggcaa tccgtctgga tcaaagaaaa acgtcatttt
    29461 ttttcctgta aaggtatctt ctctaatcgg ttctgattcg atccctaagg actgcaattc
    29521 tgcaattgtt tcttcaatat cgtctacttt gaaagcaaga tgacgcaagc cacaagcttc
    29581 tggatagttt ggtcgttgtg gcgcattagc aattccaaaa atctccaatt cagcacttcc
    29641 tagtttcaga tctaatttat agtcatctcg ttctttgcgg taattttctc tgattacttc
    29701 aaatcctaat ttattaacat aaaaatcacg tgatttctga taatctgaaa caatgatggc
    29761 tatatgatgg atcgttgtaa ggttcatatg agcactccta ttcttcgttt aagctcggat
    29821 ctatattaat ctccagttcc attgtaaatg tgtcagattg aaaaaaatag ctgtttttcc
    29881 tttttataag aaaaaaggca aaaaaaacag aaagtaggtc ctccaagaca ctcttcctgt
    29941 tcccaatata ataaatgaaa gtaagaaatt atattttcaa gaaaacaaac agaaaattat
    30001 cataacacaa attatttata attgaaagac tagttttttt tcaagaaaag catacaatag
    30061 attgtgttag taaatacaga acacactaca tttagtgtct taaggagtcc tcatacatga
    30121 agtcatattt tcagttttta gctcatcagc taaaaggctg gccgcaacaa aattattatc
    30181 ttttcttttt cagtcttggc tgccaggtca tgactcttgt caatagtccc atcacttggc
    30241 tgtccgttat tacatttatc ggtaccacgc ttggtgtatt gtgtatttta tccatcaatg
    30301 cggcaaaatc agtcaacggt attttaggta ttttgtctgc gatttgtttt attatcgttg
    30361 ggttttcagc aaagaactat ttaagtatcg gtgaacagat tgcttatatg atcacattgg
    30421 atatccccgt attgttaagt aaagaatgga atcataatat ggcttcaaaa atccgaaaat
    30481 tcactggaaa aagctggttt atcgcaatca tcagtacttt ccttgtctat cttgtttcag
    30541 gattcctgat tggtcgttat acagatgatc ctcggccgtg ggtagatgcc attgcttttt
    30601 ctattagctt gactgctggt atcatcagtt tcttgcgtta caacaaccaa tattactggt
    30661 ggttagcttc agggcttgct caaatggtcc tttggtatat ttcatttacg catggatctg
    30721 caagtttggc gatgttcgtg aacagctctg tttacttgct gaatgatgtc cttgcattta
    30781 ccgtttcacc ttggtataac caaaaagaac gtgaacgtat gcaaaaaatg gaagaagaat
    30841 actacaacgc acaacaacac gcataaaaaa actgagcgtg ggacaaaagt aaagaacact
    30901 tttgtctcac gctctaaaca cgtataaacg gcggaatcag aagcaactcc ttcggaaata
    30961 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
    31021 ttaaacgctt ctgtcccagc ctctattttt tatagtaatt ccttcagata ctttcttgcc
    31081 tcatcattga aacgcagtga ttcttctaac ttctgataat attccaacgc ttgtttctga
    31141 gcttcagtaa aacgataatg ttttcctttc catttgtcga ttgtctcttg cacatttcca
    31201 gattggatat ctttcactac tagttgccat ttttcttttg agactgtacg gtctttctta
    31261 tcagcagatt gtatagcttc tcgttcttta cgcaatactt ttaaaaaacc atttctgact
    31321 atccgaggaa tgctgtcgaa gaacccttct gtaaaaccat agcgacaata ttgcttccaa
    31381 ataaatagat agatttttgg ttggatgaat cgttggtttt tcgcaaaata ttccatctga
    31441 gcagtgactt ctggtgttcg ttgacgtttt gtctttctca acacggaaat aaatgtttca
    31501 aaataagcat taggatgtac tggcacttct tcatatacga tggattgcca atctttacgt
    31561 aaaaatgcgc gatagttgat caagaattct tctacatcct ttactaattc ttctgcaaag
    31621 tcgtcaaata gttctttttt tatctgtcgc ttttttgact gcccattgat gtgttcactt
    31681 attttcttga tttcattttc caatgcttga taatccgcta tgtaaatcgg catttctact
    31741 tggcgaaatt ctgcgagccg ctgagctaat tttatatccg gtaaatatgg ctgtttcatc
    31801 cgacgatttt ggtacaagac aaaacagtat ttttgccata gtccttcagg aaaatcgata
    31861 ttcttctgct taagccataa taattcgtct aaggccagat caacttttgt cattccctga
    31921 tggatcgaag ctgccactgt cggagagaca tgcaggtgat cggcaaagtg gttgatgcct
    31981 agttttagga tctttccggt ttttggcgat ttaacgatgt attgcacttt taattctcta
    32041 ccacactcac aatacaattt ttgctggctt cgttttgttc gaaaaaacgg attgatcttg
    32101 aagtcagaaa acacccaatc tgttgcagct aaataatgtt cttccataaa acgagagctg
    32161 attagatatc ggcggtgctt atcgactagt tcccgctgtt ttttcgttaa ttttgcatag
    32221 atctcccaac gttgactgct tgtgtattgt ttagaaattt tgatgggata gcttttgctg
    32281 atttcttttg gaaaccattc ttcaaagacg gattgccatt cttccacggc tgtttctttc
    32341 ctttctaatt acaaaaagct ggctactaac ataacgttag caaccagcca gtgtttcttg
    32401 ctaaattagt gagttgttcc ggcatttccg gaaatatctt ctttattttc tactgctgct
    32461 tcaactgcat aagtcaaaga agaagcaatg tcttctaaag acatagaagg cgtacccact
    32521 ggacgatcga tcacttgatc gggtgagaat ggcacgtgga tgaacccacc tcgaattgcc
    32581 gggaattgtg tttcgatcaa atgaagcaaa cgatacatga tatcattgca gacaaatgtt
    32641 cctgctgtat aagagatgta cgctggtaga ccatgatctt gtacattttt tgtcattgct
    32701 ttgattggca atgaggtaaa gtaagcggct ggaccgtttt cttctaaagc cgtatcgaat
    32761 ggctgatttc cttcattatc agggattctt gcttctgcta gattgattgc gacacgttcg
    32821 aacgagactg ctgaccgccc acctgcttgt ccgacacaga tcacgacatc tggttggtgc
    32881 tgttggatgg cttctttgac tacttgactg cttttttcga atacggtcgg tacttccact
    32941 ttgatgattt cagcaccgct gatttcatct ggcatttttt ttactgcttc ataggctgga
    33001 ttcactttgt ccccaccaaa tggatcaaaa cctgttacta gtactttcat ctgttgttcc
    33061 tccgtccgat tgatttaaca tatggttatc ttttatcata ccattttcct tgaacagcgt
    33121 aaataaagga aacaatagtt taaataacat aacagatcaa acttaatata ttcttataaa
    33181 aaaatagtgt tccactacaa tgttagaaga ggttgcgaca attattctgt cacaacccct
    33241 ctcttctatt atttaaatag aatcatataa gcaatttgga aaactagcat aaataacgca
    33301 ataaacaact ggtttttgat cactccgtat ttgtctttca tctccagcat cgctacaggc
    33361 acgatattga agttagcagc catcggtgtc agcagggtcc cacaaaaacc gcaagtcaat
    33421 gcaaccattc ctattaacgc tggattcgct ccatgactaa atacgaatgg tgcaccgatc
    33481 ccaaccgtca tcacggtgat ggccgcaaat gcattcccca tgattaccgt aaatatcacc
    33541 atgccaagag cgtaaatgat gatgccgatg ttcacatttc cttctggaac gatcgtacct
    33601 actgcattgg aaatcactgt cccaactcca gcgcttgtaa ataccgcacc tagagaagct
    33661 aaaagcattg gcaacatgct taacggccct actgtgccca gcatatctgc tgcatcatct
    33721 aaaaatgtac ttggtcgatt atctcgagaa aagaacatca agattagcat ggctgcaaaa
    33781 acaccgacgc ccacgccgac taaagcacct aaattggtaa atagagcaaa caagatcgca
    33841 aagacaccga tactcaaagc tggaataaaa attttcattc ctagtttgtc tgacattttt
    33901 tccatatatt cttttgatgg aagccggctt tctcctttgc tgactttctt agcaattgct
    33961 ggtatggtca tcgcaaagat caagccaccg ttcacgattg ccggaagcca tcttcctcct
    34021 gcaataacta cccctaagac cgtccagaaa aaggctgacc cataacgatg aggattggtt
    34081 ttatctgtta aatttcttac tccagtgtaa atcaatacga gtcccatcac gatataaagg
    34141 atctccagta atttatcgcc taataaaata tcaggatttg taaaaaagtc catcttattg
    34201 cccctttcct ggataatatt tcttcgctaa tttcttttct ttaagataga agtagacact
    34261 tgccacaact aaagccgtga tagccactgg aacttccact aaagcaagct gtcccaatgt
    34321 tacgtcatat cctagatctt tcagtgttga ttggactagc aacgcccctg ctcctccgat
    34381 gaatagtact tggccaaaga accagcagac attttccata gctgaagcca taccttttag
    34441 ttcttcttca tgcttaggat ggacttctaa cccttttgat tcgatggtcc ccaatgccat
    34501 cggtaaaatg attggacgaa caaatccagc aactccacca aaactcacgt taaaagccgc
    34561 aaagatcatt cgaaagatcc cataaatatc tacaatcata ccagcagata ctttcttgaa
    34621 tcgcttgatc aaggaagccg ctgattcttt cagaccattt ctttcaagcg ttcctgttgc
    34681 caacataatg atgataaaaa tcgccatccc gcgattgttt acaaaacttg tacctaaaag
    34741 ttctaacatt ccttgaatgc caagaccgcc agtcaaagct gtaacaacta aagcaaccat
    34801 tacgatcaat atcgagtcta atttaaatgc aaacccgaga ataacaatca agacgcctaa
    34861 aaggctaaga taattttcca tatattttcc tccataaaat gacaatattt ttttaattat
    34921 aaagcaaaac acataaaaaa tatagttgtt ttctcacgaa atagacacta tttttaaata
    34981 tttttcgtta tttttttatc acaaaaatta tcttacttat tgtaaggtaa agcttttttt
    35041 aatttattgc ccgataatat atagaaataa ggtctagtta ctgttttttt taaatgatta
    35101 ataaataaca acttctttct atgaaattgc aaagcattca tcttcttttc cgctaacgat
    35161 atatccgtca gtgcttggta cactttacta aaaagtatga gattgccgat atctggatag
    35221 gtgtgctata ctacagaaaa agatatacga tctgccaccg ggtaggataa agaggatgat
    35281 cattccatta ggtcttttaa gggatgattt tcctcttttt gtgtattttt ttatagttaa
    35341 ggaggaatgt aagtatggca tggatggaat taatcatagc aggaattttg gaagtattct
    35401 ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag
    35461 tgattggcat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga
    35521 gtctctctta tcctatctgg acagggattg gcgcagttgg ttcggtcata attggtgtgg
    35581 tcttgtttca tgataagctg aatctttcca cttggttctt tgtggggatg ctactcatca
    35641 gtatcattgg catcaagatt actagcggcc actagacact tggaataagt actgatcaga
    35701 aaaaataagc ctttttattt aatattcaat ttttttatga aaaaataaga aataagtcca
    35761 atgacctagc gacttctcca ctcatcaggt gtatgcttgt actatctata gaaaaggagt
    35821 gaatattttt gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag
    35881 ccctcagcga tgtcagtttt agcttatccc ctggcaaaat cgttggatta gtaggtccta
    35941 atggcgctgg aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagatg
    36001 gaacaatctg tattggtgaa aatgagattt ctccctctag tcataaaggg ttggaacaag
    36061 taggagcatt gatcgaatac ccgggtatct atccattctt aacaggctat gatcatctga
    36121 agttattctc tgaaaccaac gatgtatctg ctatcgacac tatcgtgaat cagctcaaga
    36181 tggaaaagta cattcacaaa aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg
    36241 gaattgctct tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg
    36301 gtttggatcc acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg
    36361 gcacctcttt cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc
    36421 tgatcctgat caatcaagga aagatcatca aacaatgtac aatggctgaa ttgaaccaaa
    36481 cagcgcaaga tttcatcgtt atctctacaa caaatgatct agctgctcga acgatcttga
    36541 cagaagctgg atatactttg gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag
    36601 aattattagg aaccgtgatc cagctattgt ctgcacaaaa tatccatgtg acagacgtcc
    36661 agcatctgac gaatgatttg gaatcttctc tattggaaat tctggattca gtaaaggggg
    36721 aagaagaaaa atgacattgc ttgtaaaaca agaattattt aaattgatca agaaaaagtc
    36781 aactgctgtt ttatctgttc tattagttgt tttgctcata ggaacagcac ttctagccaa
    36841 aaaatacacg acgatcattg atccagttga aatgactgct cagcttttta gtgccacatc
    36901 ttggattgtc tttatcatga ttgcagctgc aagcacgatc atttcgatgg aagcacaata
    36961 tggtacattg aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagcaa
    37021 atggatcaca ttagttattt attctgttta tttatacctt ttagctatta ttgtcactgt
    37081 gttgatgaaa ctgattcttt tcccttcgat ttccttcacg gagaaggttt ctactggtca
    37141 aacactgatc caaagcttgt tcacttatac tttgggtagt tatatcggtt tatggctgat
    37201 tttaagttta gtgttgatga ttgcttgctt tatcaatagt tccggtgcat ccatctcagc
    37261 aggaatcgtc ttttattttg cttcgtcaat catatctggt atccttattg cactcatcca
    37321 aaaatgggag tggatcaaat ggaatccgat cagtatgttg aatctgcaaa accaaattgg
    37381 aaacgaagag atcatgaagc aattgactca tctttctact aatcaaatgc tattcggtaa
    37441 tttagcttac atcgttttgt tcttagcatt aggatatctc gtatttaagc gaaaaaatgt
    37501 atgatgactc atcaaaaaag aggctgtgaa agagacgttc agccttattg ccgattggct
    37561 gtctcttaaa caccgtttat tctgaaaaag gagctgtgac aacatttgtg tcacagctcc
    37621 tttcctagta tgtttttgaa taaacgacag gctcacaacc tctctttata cttcttttat
    37681 acttctatac gtgaagcatc ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg
    37741 tcacacctat aagtaaaatc ttatctaagt ggatatgttg tacaggctcg ttttgctcct
    37801 tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag aacaagaaca agcattactt
    37861 cattaaaggc tacgactagc atagcccata cttgaaaggc acttgctaaa agccctacta
    37921 aaaattggac ccattctttc cgctgataag aaagtttcat ttggtacaat cctaagaaaa
    37981 gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc aaagttataa gcactatcag
    38041 tgaataataa tgaaagtaaa aagacattcg tacataacgt cgtcaatacg atagcataag
    38101 ttggtgcccc atttttgttg agttttcccc atgctttagg caaaagtccg tcttcagcca
    38161 tgtttcccat tgtttcaatc ggtaagatcg tccatgagag ccaaacacca actaaagaaa
    38221 tgatcaaacc gccgttgatc acgactgctc cccatttacc aaccatcatt tcaaacacat
    38281 agcccatagc aggctgcgcc ttgatcgttt gcagctgatt catcgtcatt gtcccatatg
    38341 gaagcatgga aaccaagacg taaataataa tcaagccgaa tatccctaga atcgttgctt
    38401 tcccaacatc tgattgtttt tttgctcgtg aacctacgac agaggctcct tcaataccaa
    38461 caaataccca aagcatcacc atgatgcaac tcaatacttg actggaaaac gggatcggct
    38521 gtccattcgc attcaatata atgtcgctag aaaagttatc aatgaattgt tggatcgaaa
    38581 acgtgctgat agctactaca ataaataaaa ataatggcac taatttacag aatgtgacga
    38641 tagcattgac gaaactagca ttttcgactc cccgattcac tagccatgca tatccccata
    38701 aaaagactgt actcatcacg atcgatgcca gattttgccc accttcaaat actgggaaga
    38761 aatatcctaa tgcgctcatc aacaatgttg caaaagctac gttgcctaac cagcatgtga
    38821 gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac
    38881 taaagatccc gccttttaaa tctggacgtt ttttattgat attattgatc gacaaggaga
    38941 gcatcaacat tgctgttcct acgagtaccc aactgaaaat agctgggccg ccagccgatc
    39001 cggagatgtc cgtcataata ccaaatacgc cacctccaat cgctgaacca attactaagg
    39061 cagcaagtgg aattaaacca attccttttt tctgttgctc tgtatccatg taagcatcct
    39121 ccaaaataaa accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta
    39181 ataaaaactt taatagaaat ataattctac cggctttttt ctcattatac aagcaaagcg
    39241 cctagtccct ttccaaatta caccaccaaa aaacttaaaa aagatgctat actaaaagga
    39301 aagaattgga ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc
    39361 ctttgccaaa catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc
    39421 tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt
    39481 ttttactttc gttcctactt atttagacgg cggaaatggt ttagataacg gtgatacaga
    39541 aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa gacaatcata aactttgtct
    39601 tggtgtagtc ggtagcggaa ataaaaactt caataatcag tattgtctaa cagccaaaca
    39661 atatgcacaa acatttggct ttcctttctt ggcagattat gaattacggg gaacaccatc
    39721 tgatgtgaca cgaatttatc agattttagc agaaaacaat aaataataaa aaagaagaag
    39781 ctgaaaaata gatttcagtt tcttctttcg tttctattgt ttctatcata ttaagctttg
    39841 acatccatca caggtgcttt tgcttttgct ttacctgttt tttccaagac aaagtttttt
    39901 acacgatcca tattggttac caagacgatc atggatgttt ctttgcctgc tgctttgatc
    39961 gcttctaagt cagcttctgc tacaagcgta tcaggtgtca cttgctgccc ttctgttact
    40021 ttcacatcaa atggcgtacc gcctaagtca actgtgttga ttcccatatg aagcaagatt
    40081 tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg ttgggaagac agagctgact
    40141 accccagtaa ctggagaata gatatgtccg ttttctggtt ctacaaagta gccttcaccc
    40201 atcatctttt gtgcaaaaac tggatcactt gctttatcta aagaaacaac tgttccgtca
    40261 gcaactgatg agaggatagt cacactttca gctgaagttt gaggtactac tttttcaggc
    40321 acttcattta ctgtggctgt ttcagtcgct aattcttctt tttctaaacg gtctttagga
    40381 ataccgaaga agaatgtagc aacgaagcca ccagcataag ctgccaataa tccaaggatg
    40441 tagcctaacc atttaccgtc agagatcaat gggatcaaag cggctcctga aggtccaata
    40501 gcgatagccc cgacatttcc gatcattccg atcactgccc cgccgatacc tccaccgata
    40561 caagcagtaa taaatgggcg tcctagaggt aatgttactc cgtaaatcaa tggttcgccg
    40621 attcctagga tcccgactgg taaagctcct ttgatcattt ctgttaattc tttatctttt
    40681 ttgcagcgaa tccataatgc caatgctgca cccacttgtc ctgcaccagc catcgcaaga
    40741 attggaagca atagcgtacg accagtttca tcaatcattt gcacatggat tggagtcaaa
    40801 atttgatgca gtccgaacat aaccatcggc aagaataatg tgcctagaac gaagccggca
    40861 aatgctccac caacgccaag tacccagttg atcccaccga ccatgccatc agaaatgaaa
    40921 cctgctaaag gcatgatgaa gaaaatttca atcagaccga tggcaatcaa tgaaagagtt
    40981 ggtgtaacga tgatatctac cgcatcaggt actactttat gcagtttttt ctcaacgatt
    41041 gacaatagcc aaacagcgaa gatgacacct aggatcccac cttgtccagc tgcaagagct
    41101 tcaccagtaa ataagttttt cagtggtgct tccggattca tccctgtcag catcacaacg
    41161 gccccgataa ctccaccaag agttggtgtt gcaccaaaaa cttgtgcagc attgatacct
    41221 gtatagataa ctaggtagct aaacatcccg tttttcaaga tgttcatcac atcgatatat
    41281 tgttgccatg ttgctgcatc aagtgttccg gctgtgacta agtttgctaa aactgcggca
    41341 atccctgcca cgatcccagt accgacaaat gcaggaatca ttggcacaaa gatattagaa
    41401 atgtctttta ataccgattt gattttagat ggtttttgtt tcgatttttg tgccgctttc
    41461 atttcttgtg cccgttcgtt tacacggtct tttccagatg ctgatgaatt tgctgtagct
    41521 gcacttacag aagaaagttt ttctcctaat ttcacgcctg ccaaatcaac catttgctgt
    41581 gctactttgt tcactttccc aggtccgatg atgacttgaa gctgttcatc ttctactaca
    41641 ccaagcactc ctggaatcgc ttttaattca gagacattta caagatcatc attgatgatc
    41701 ttcatacgca cacgagtcat gcagtggaca atgctttcta cattgcccat gccgcctgcc
    41761 cctttataaa tttgttttgc cagttcttcc aaagtcagtt cgttttttgc catggtcttt
    41821 tccccctcat aaggtccctt ttacaaatcc gtttgcttca gctaattttg cttgagcttc
    41881 ctctttatca ctatttgtca aaatcatgac gatagccagc ttaacatttt gatctgcttc
    41941 ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc gcttgcatga tgattcgttt
    42001 tgagcgttcg attagttttt catttgttgg tttgacatcg accatcaagt tattgaatac
    42061 tttacctata ccaatcatac tgatagtaga aatcatattc aagataagtt tttgtgctgt
    42121 tcctgacttc aatcgagtag agcctgtcaa aaattctgga cctgcatcga cttcgattgg
    42181 gaaatccgcg tgtttactga tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc
    42241 accgatcgat tttgcgtaat ctaatccacc gatcacatat ggtgtacgac cgctagctgc
    42301 gataccaata actatatcat tttttgtcaa attcagcttg attagatctt cttgccctag
    42361 ctcataagaa tcttcggccc cttcgacagc tacagtcatt gcttcttctc caccagcaat
    42421 tagacctatg accatttccg gttctacacc gaatgtcggt acgcattcag cagcatctaa
    42481 cacacctagt cttccacttg ttccggctcc catataaatc agacgtccac cttttttaaa
    42541 ggactcaatc gtgttagcaa tcactggttc gatacttgtc agttcttttt caaccgcagc
    42601 ggccactttc tgatcttctt tgttcattag ttgaacggct tgagaaatac tcatctcatc
    42661 taatccaaaa gtttcttggt ttctgcgttc cgttgttagg ttctctaaat tgattttgct
    42721 catttttatc ttccttcttt tcttataaat tgatagttca taccagcttt gatttggttg
    42781 atcaatggta gctcttcttt gataacttta gctactacat tcactttttc atccgctggc
    42841 aaatcatatt tggttaattg gatctcaccc atatagcgca aatacttttc gttatctaaa
    42901 gtaatagacc ctttcatacg cgtagctgta tttcttgctg ggataggcgg aatctctcga
    42961 aaacgtgcat ctgcgcttcg tatgacatct cttgcatcgt cttgccgatt cgtatgcttc
    43021 cctaatatat attcgtaaaa ttcttcatct acaggttccg tatggagcaa gactgtctgt
    43081 tctttttgga aagaagcaaa ttgctcctga acattttctg acagacctgc atctccgatg
    43141 taaacaatat ccgtgttact tgctaataga tctagagcgc ctgcaagagg atggatccct
    43201 cgatgttctt ccaaagttgg caatccttga tatagtgggc ctctcaactc tgtatctcct
    43261 ggtacaaaac cttggacaag gagaccttga attttgagcc attgattttt tttctgatac
    43321 cattctttgt ccagccctgt ttcgggacgt ggataataat tatgccaagc ttcaatctgt
    43381 gagaaatcag cttctgcttc tttcaattca tcgatatctt taggcgtgat cgtgctagca
    43441 ttcaaactga ttttcaattg atgtgaccag tcggcaatct gctgattgga aatatgataa
    43501 tccattctta aacctgttac accaattgct ttcaaaggcg ttagatcttc taatgaaaaa
    43561 cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca attctagttt attctttttc
    43621 gcttcgcttc ctagttcgat caatcgcttt tgatatgccg aaacatcatc ttctggtata
    43681 tgcatggaag tgaaaatacc gacaaagcca ttggatgcca tttggcggat atactgtttc
    43741 ttctcttctg acaaagaatc gttcataaat attgaaaagc caaacatatt atccctcttt
    43801 ttctgtatcc gtcttcattt ctatgactag agtatatatc taaagaaaca aaatttcaat
    43861 acttttatta aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat
    43921 tgtcataacc taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa
    43981 ataacacaaa aacataatta ctcataagaa accaattctg tattatctac catgaatgta
    44041 aagaacccct cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta
    44101 tgacaaacta acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg
    44161 atccgtgcat acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa
    44221 cgtgcttttt gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt
    44281 tctaccaatc gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct
    44341 gtatctcttg ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct
    44401 actacctttt ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct
    44461 gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa
    44521 agcatgtgat tctctccttc gttggcaaat ttccccttaa ccataccttg ttgttcttga
    44581 gattacaagg gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag
    44641 gtttgatcat gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat
    44701 tgttcttgat tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct
    44761 ggctgtgata aagatttagc caattccact tttttccgtt ggcccatact catctgctcg
    44821 atcttgttat gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct
    44881 tggtagtcta cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa
    44941 ttttggcgta cataactaat gctcagatgt tttggctggg atttttctcc tataaccttg
    45001 ccgttgaatg cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct
    45061 gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt
    45121 tcatacccta attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta
    45181 ttcatcgtca aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc
    45241 tgggtctcca tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca
    45301 cccactgctc ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt
    45361 gttttatctc cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc
    45421 ctactgactt cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt
    45481 ttctgttctt catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt
    45541 gctaaaacat ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag
    45601 ccttgttttt tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga
    45661 ttcgttggtt catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg
    45721 acttttgtct tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct
    45781 gtttgcatca attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt
    45841 aacacgtaat aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct
    45901 tgctgatgga ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt
    45961 gtctttcctc gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga
    46021 tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt
    46081 tcgattttag acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg
    46141 ctaaagagga tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc
    46201 caatcccgaa aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct
    46261 ttcatcaatc gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat
    46321 tacgatagca taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc
    46381 taagtgtctg tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt
    46441 ctaaacctat tggacccaaa tgaatactta agacaattgg gagaaaatca gagagtcctt
    46501 ctagctcttg gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa
    46561 gcactgctac accgtgtgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc
    46621 ctgtaatgat gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta
    46681 aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg
    46741 ctgctccact tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag
    46801 caatctttcc tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc
    46861 ttccaaattg ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga
    46921 taatcaattt tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag
    46981 ataataaagg atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc
    47041 ctagaccacc atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt
    47101 gaagcagtgc atctttgata agttctccta aggcataact ggaagcaagt cggatagttt
    47161 gatccgttgg tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg
    47221 tattttttcc ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataaaagat
    47281 ccatcccatc aatggtacgc catgttcccc cgagcgcgta atagattgct tctgcagtat
    47341 tttctccgcc atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat
    47401 ttagaactgc ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg
    47461 ccaaaatatc catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt
    47521 ttcaaacaaa acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga
    47581 taaaggagag aaaaaacatg aaaatcgcaa tagctggagc cggagcaatg ggcagccgaa
    47641 tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt tggcctgcac
    47701 acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa gaagtggtcg
    47761 caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac gtggacttga
    47821 tcgtagcatt aacaaaagcg aatcaattag atgatatgtt tcgttcgatc caatccatta
    47881 ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa gatgtactgg
    47941 aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag
    48001 ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag aacttggaac
    48061 cagaaggcga agcatttaca aaaaaagttg tggaagtctt tcaagaagca aaattaaatc
    48121 cgatctatag ccataatgtt cgttattcca tttggcgaaa agcttgtgta aatggaacat
    48181 tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg caaaaaacgg
    48241 cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca aaagaaggaa
    48301 tcatattaga tcaagaagaa gtgtaccaac atattgcttc tacttataat cctgacaaca
    48361 tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa
    48421 tcgactacat caatggtgcc gtttggcgaa aaggacaaaa atatgatatt gctacaccat
    48481 attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa
    48541 aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta agcccaatga
    48601 aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg tttgtttcgg
    48661 tacataatct cttcaaaatg taggtttttt atttattttc aacagaggag aggtcatatt
    48721 cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa ttttctgcat
    48781 cataaaattc caatagctct gatgggattg gcttaaaagg tgtatgcaga aattccggta
    48841 attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa aaatgataat
    48901 gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatagat tcaaatgtcc
    48961 gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc gattcccctg
    49021 tttttttcag gtactcttca taagcaagat atccaggttg ttcattccct cgatgtggaa
    49081 gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag ctttctgctc
    49141 gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt tcgctagaag
    49201 aaaaatccat atgtttcgaa taaaatgaac tattatgttt cagcccaagt tcttttgctt
    49261 ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca
    49321 tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt gcattggtaa
    49381 atgggcgcac gcgtcgagcc atctccatat taaatccata atctaaaaaa acgatcagct
    49441 gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt aaatcttttc
    49501 cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta ttttttgcaa
    49561 ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca ctttttccta
    49621 tgatagcctg tttaaatagt aaaaagccag taaaacgctt cctatatttg tatgaaaatt
    49681 taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt cgcctaaaag
    49741 ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt taaatattgc
    49801 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
    49861 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
    49921 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
    49981 tattgattgc ttttttgatt ggcgggctga ttttatatat cgtgatgaaa ggtctgggaa
    50041 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
    50101 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
    50161 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
    50221 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
    50281 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
    50341 taatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
    50401 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
    50461 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
    50521 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagccat caaaggtgtc atgggaagaa
    50581 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
    50641 tagcaggcac gaatgtcagt ccttttgtga ttgtatttga aaaaatgaat atcccatttg
    50701 cagcggatat cgtcaatttt gttattgtcc ttgctttgtt ctcatctatc aattcgggtg
    50761 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
    50821 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
    50881 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
    50941 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
    51001 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
    51061 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
    51121 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
    51181 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggaacta
    51241 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
    51301 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
    51361 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
    51421 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
    51481 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
    51541 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
    51601 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
    51661 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
    51721 cattctatcg ctatcctgac aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
    51781 ttagcggaag aaatcatcca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
    51841 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagtgatc
    51901 agcttcaaga aataagaaga agctacttct ggaacaccgt gaataagggg ttgtgaaaaa
    51961 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
    52021 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcgt aaaagggatt
    52081 gtgacaaaaa aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt
    52141 cttgcttact gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc
    52201 actgaccatc gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag
    52261 tgtaggaata ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta
    52321 gttgattact gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc
    52381 tttttcataa gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc
    52441 aataaaaata tttactccga tacttgtcat caatgttgta aaaatcaatt ttggtgtgtt
    52501 cgtcatcttt cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa
    52561 tccacctaat gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc
    52621 tccgcgacta agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc
    52681 gatgacccca gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac
    52741 tgctacagcg atattcaaca aaatcgtcaa aattgccggc acctttttcc atgcacacag
    52801 taacattaaa aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac
    52861 atcagtcact tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat
    52921 gaataaaaag aaagaaacaa caaatgccgg aacagttgac cacatcatat gcttgatgtg
    52981 tttgaacaaa tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga
    53041 catcttatct ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttag
    53101 gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc
    53161 actccctaca gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg
    53221 gacactgatc aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca
    53281 tacagcaatc aatgaaccta ctaaaataaa aataaagatt gggatgattc ctggtttgat
    53341 gccattgatg attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa
    53401 catgaccatt ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac
    53461 gccaatcgca atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg
    53521 tgttcctttt tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag
    53581 ttaagatatc ggattactcc gccgcaattc atccgctatt cctcctttat aaaaataaaa
    53641 agacctgttt taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc
    53701 tgtttttcac tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta
    53761 ggcttgatcg gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg
    53821 tgaaaagttg acttgtatga agtttacctt ccataagatc atttgtcaat gaaaaagaaa
    53881 atattttttc atttttctga aaattatttt tataactttc acacacaagt ttatttttta
    53941 ctagaaagta gcaccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat
    54001 tctttctaat agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc
    54061 tgcaatcgca atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt
    54121 gtgtccggca atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg
    54181 gctgacattt cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc
    54241 atctggaaca cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac
    54301 gatgacatca tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat
    54361 cgcagcacct cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt
    54421 tcctgcacgt aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc
    54481 tccgtctgta atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt
    54541 gattccatta aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc
    54601 tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc
    54661 tgtcgttttc ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa
    54721 aaaaccttct cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg
    54781 ggtgccatat acacggattt ctccaccagt ttcatcaata aattgctcca ctatgctggc
    54841 ggctaatacg gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa
    54901 agcatcgaat tctgccaaaa aggcaattgt gggtccgggt ttattggaaa tataacgtgc
    54961 atcaaaggct gttcggtgtc cagctacttc tttttctacc caaaaccctt ctttttctaa
    55021 ttgtttaatc aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg
    55081 gatatctaga gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg
    55141 gtttgttgag attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt
    55201 tctcccgaat gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt
    55261 gactgtgtct tctgtttgat aagcgtctac tattttttga tacgttttat ttttttcatc
    55321 ttcttttctt gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa
    55381 gaagatggca tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac
    55441 tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc
    55501 tagatttttt ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc
    55561 cactttgatc aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc
    55621 attagggatt gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga
    55681 ataaattccc agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc
    55741 tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc
    55801 tgctaaagcc gcatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt
    55861 ctcctttttc aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataattcc
    55921 tagttttacg gtagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc
    55981 caaccaaata attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc
    56041 tcctccactt tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa
    56101 aaactaagat tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat
    56161 accctctcga tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag
    56221 tcagtccgat cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt
    56281 ctaacaaata cacacgtaaa ataataccga ttggactggt tcccatcgct tctgccgctt
    56341 cgatcacccc aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa
    56401 caactccaaa aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga
    56461 aacgagtagt caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat
    56521 tgatcagctt atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg
    56581 tcactactaa cgtcacacct aagatgattc cgatcatccc gccaatgaat gctgtccaaa
    56641 aagacatata tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat
    56701 tgggcaaata tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata
    56761 cgactatcta attcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa
    56821 tccaatgctt tttgccgctg tttggtttct ccagctaaag taacgatcaa actccctaac
    56881 ggcgtttctt gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg
    56941 ctgtacagat gagcgattag aggttcgttc gtctgatttc caatataact taattccacc
    57001 agccatttat tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct
    57061 tggtcaagat gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg
    57121 aagatctgga tgctactacc tttttcaatg attcttccat cttccataac cgctaccttg
    57181 ttgcagattt ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac
    57241 tggcgattca atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca
    57301 ctggttgctt catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct
    57361 actcgttgtt tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa
    57421 ccgacaagtt ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa
    57481 tactttaaag aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc
    57541 tgaaagatca taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc
    57601 aaattcgtct gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca
    57661 cgaactaacg tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt
    57721 tcaatcgtta aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat
    57781 acagaaacat ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca
    57841 agaaaatgaa aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca
    57901 agctgtgttc acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg
    57961 cagccgtcat cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata
    58021 gtcgttcctt gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact
    58081 taccatttca atgcaaataa tattcaaaaa aaaatccttt tactaacata aactatgtca
    58141 gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc ctcaagccca
    58201 acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg aatttgccta
    58261 cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac tcttctcacc
    58321 agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa atcgatattt
    58381 tgttgttggg attaatctac atgaggaaaa ctaaaaagtc aagcacgaaa ttcaaattat
    58441 tttttctata tttatttttc tgttttttta tctaacattt caataacagt tcgaagatct
    58501 tttccttcag caagaagttt caatttttcc ttttcttcga cggtataatt tcggacaaaa
    58561 ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt tgctcctact
    58621 aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc caaagctgtc
    58681 agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt aaacgtgtca
    58741 aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt aacagtggtc
    58801 gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc ttttgcatat
    58861 ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc acgcttcgct
    58921 cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc aaaactttgt
    58981 ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat atcaaattgc
    59041 tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa caaaccatct
    59101 aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat acacatagtt
    59161 tagcacgact ggtccaaaaa taacagaagc aaagaaccag ccttctcgtt tataagagtt
    59221 cttggaatcg atggatatag attttcttca cagctgtcac aagcgccaga taagcgatga
    59281 ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct aattcagcac
    59341 caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg ataacaaaca
    59401 tggcaaatgt tacgttgctt tggataaatg gtactttcgg tgtacgcagt gcatggagca
    59461 ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat gcgatgaaaa
    59521 tcacttgctg tgaactatcc aatgtatgga aacttccgcc aactacagca ggacaaataa
    59581 caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttggg ccaaagtaaa
    59641 ccatgaattt cccaatacta gatgcattcc atttttttgg ctcgtccaaa tattctttat
    59701 ccatccgatc ccatggcaag gaaatgcagg aaacatcata gattagattc aaaaacagaa
    59761 gttgtaaagg cagcattggt aaaaatggca ggaacatact tgcaatcagc acggaaaaca
    59821 tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc ccaaaagttt
    59881 ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt
    59941 ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata tcagccactt
    60001 tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga ccatttttac
    60061 gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat acttcatggt
    60121 cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca ccggtaacca
    60181 attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat tcgttgtccc
    60241 cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct tgtttcgttg
    60301 tttcttttgg tggatcaagg aaagtaagat aaccaatcaa aaccatgtct gactcatctt
    60361 tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca atcactcgta
    60421 agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt tcttttgtta
    60481 atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt tcttcaatcg
    60541 cgccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca ctcatacgac
    60601 gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta tctgtatcta
    60661 aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat
    60721 agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca
    60781 tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc gtgcataaca
    60841 catcgattgc accaaagttt tggatggcat tcaaattttt gatcactgtt ccttttttgg
    60901 ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggtg
    60961 ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa
    61021 gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc agtagcatgg
    61081 atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat agcgtttctg
    61141 ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc gcacttccac
    61201 ttatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt tgctgatcgt
    61261 cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttggg
    61321 aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata tcccctgctg
    61381 aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg tactttccat
    61441 tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc gcttgattgg
    61501 aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat gaccatagtg aagataatgg
    61561 ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg atgaatgaaa
    61621 taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc aatatttcta
    61681 cgatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt tctcttgaac
    61741 gttcgatttg ttcttctgtc aatccatttg ctgatgcgtg gaatagttca aataattctt
    61801 gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct
    61861 caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct ttagctattc
    61921 atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat agccttggcc
    61981 tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact
    62041 aagtaatttt aattcatgac ttccaatcat ttttcgaata gttattttcc caattagacc
    62101 acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat
    62161 actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc atcgttgagt
    62221 tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat gaaaagcctt
    62281 atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc agtagcctat
    62341 gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta atataacaac
    62401 taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta aaatacacac
    62461 gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg ctataataga
    62521 aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc aagccagacg
    62581 gcgtgagacg ccatttagtc ggaagcatca ttcaacgttt tgaagcaaag ggactggcca
    62641 ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat tatcagcact
    62701 tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt cctgtcgttt
    62761 atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt ggggcaacaa
    62821 aagctgtcga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg gggactgaaa
    62881 acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc
    62941 cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg
    63001 ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga cctccgttga
    63061 ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt cgtctttttt
    63121 tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt ttcatagatc
    63181 actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac actacgtgcc
    63241 tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa agtaaagaga
    63301 tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat tttcaaataa
    63361 ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat agatagttca
    63421 aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca ttcataacgt
    63481 aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag actcaaaacc
    63541 aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat ctctttataa
    63601 ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt taactttatt
    63661 ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt cattcgctct
    63721 aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc aatcccgact
    63781 agagaagtct tagaacagat tccttccgct aattgggact gtgtcaactt cttgttgact
    63841 cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa tttaaatttt
    63901 tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg
    63961 tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact actgaaggtt
    64021 ggtttgattg tttattgatt atctttttgc tgttcaacgt ctgttcttgt tactggatat
    64081 aagttggttg ttgttttatt gccatttttt gaaaagacac ttgtcgaatg ttcgcctttt
    64141 tgcttttcga tggctatgtt acgttctgct tcatttttat agtcatattt tttcggatcg
    64201 atcgtttcaa atccgtttgg cacataaaaa cgcaagagat tttcttgatt cagcttatct
    64261 gaaagactta aagcagattt gactttttcc tgatctttgg aaatttcctc gtcgattcct
    64321 gctgctttcg tatcgatgat ttctcctgtt tggttattgt aagcttttcc tcctaagatg
    64381 gtgtatttgg gtgtcacgaa gttcccattt cgaaaagcca ctacttgatc atgctgagga
    64441 gataataaat ccgtaccaaa atggagatag tctttatcat cgattcccaa gagatgaagt
    64501 aatgtcggca aaacatcgat ttctccaccg tattcgtgat tgactgaccc tttagtataa
    64561 ccaggtatat gaatcattaa tggcacacgc tgcatttgtg cgttgtcaaa ttcattccat
    64621 gtatctgggt cttttccaag agcacttgct aagtctttat tatctgtatt tgagatgcca
    64681 aaatgatccc cataaatcac aaagatcgaa ttttggtaaa tagcggattt tttcaggtac
    64741 gtaaaaaatt gttccaatga ctgatccaaa taatgtgccg ttcgaacata gttatctact
    64801 gtgctatttc cagtgttcaa agatggaaaa tcgaaattct tatcatccgt ataataaggc
    64861 gtgtgattgg ttacggaaag gaatttcgtg tagaacggct gttgtagatg ttcaagatat
    64921 tgggcagatt cactgaataa atctttatct aatattccat aacctagcgt ttcatctgat
    64981 tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct
    65041 ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt
    65101 ccaaggatcg ctggtgcggc ttgaaagaca ttatctgacc caagttctgt gaataatgag
    65161 ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc
    65221 tgtcctactt catgaaaaaa attatcaaag ctgatcgttg cttgattctg gaaaatacta
    65281 tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa
    65341 ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt
    65401 ggctctgcat aatgtttctt tgtataacgc cgattgtaaa attaagctag acaactaaaa
    65461 aagtcatttg tgctacactc aaaatagttc cgtcgaaaga attataagga gtgagtacaa
    65521 atgacctata cccatcttac accaactgaa ctcgtaatga tagaagctta tttcaatcaa
    65581 tcacactctg tttcaaaagt agcccatctg gtacaacgtt ctcgacaaac catttataat
    65641 gtctatcgtt ttcttaaatc tggtggaagc gcaataagct actatgaaca atataagaaa
    65701 aacaaacgta actgcggaag acgtccgatt gtgttaccag atgggcaaca agaatacatt
    65761 caaaaaaagg tggctcaagg gtggactcca gatgtcctta gtggtcgagc agagtttcct
    65821 attgcttgtt cggtacgtac actatatcgg atgtttaaag ataaaacctt tgaccctcac
    65881 gatttaccaa tgaaagggaa aagaaaacca aacggacata aagaaaaaag aggaaaacaa
    65941 gcttttcgcc gttcgattca ggatagaaca actgattata tacagttcaa tcatgagttt
    66001 ggtcacctag aaggtgacac cattgtcggt ggaaaacata aaagtgcggt gatcacgtta
    66061 gttgagcgat tatctaaagt gatcatcacc ttgaaacccg ctggtaggca agctatagat
    66121 attgagaata gactgaatca atggtttcaa tctgtaccga aacacttgtt taaatccatc
    66181 acatttgatt gtggaaaaga gttctcgaat tggaaaaaga ttagtaatac caatgatatt
    66241 tcgatttatt tcgctgatcc aggaacacct tctcaacgag gactaaatga acattcgaat
    66301 ggcttattac gtaaagatgg cttatataaa gctatggatt ttaataaggt atccgaaacc
    66361 tttatccaat cggtggcatc aaaacgaaat catattccta gaaaatcatt acactatcgc
    66421 acaccattgg aagtattttt gagttacgta ggtaaagatg atttgtctag cttaatttga
    66481 caaacgaaat aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg
    66541 ctagtcattc ctgttttgat tccatcataa atagtatacg catcgattcc tagatatttg
    66601 acaatatatg aacggtcaaa tgttcgctgc agcaattgtg gacggttggc ttcgctcagc
    66661 gctagatttc ctgaaaaaat aagccctgaa agacaggtca cagcgatagc catcggtttg
    66721 ctgacaggtt gacttttgat aggtattttc ttgatcgtca accaaaccag tatcccaatg
    66781 aacgtagcaa tatccagcca gtagaggaca tcatggggtt tcatcaaaga aaaggagctt
    66841 cctgataacc cttgcgacac tttcgaaaat cccaatacag atttgactgt aatgaaatct
    66901 gtgaattccc gataaaaaat aatgttcaag tacaacaaaa gcgtgtttaa aatatcaatg
    66961 atcaaaagaa tggctaatgc tggctttggc tttttaatat acaaagaaag gccaaaaaac
    67021 aataaggtag ttgcaatcgg gttgatccac aagataaagt actggatcgt accttccaca
    67081 cctaacgaaa aatctgcata gtaagcaata atcgtcttca tccagaaaaa gaaaacagct
    67141 gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact
    67201 tctctctatt cacatttttc cttattttgc tcctgaaaaa tgaaaattcc ctaaaaaagt
    67261 tgttatctta agattttttt attctttcta aagattattc cgtgcacttt tcacagcata
    67321 aaaccgctgt gatataagcg attgaaacag taaggtgata aaagtcttgt cgcagctcct
    67381 gtttagttga tgcaggacag ctttttcaca acttcttctt taaacggtgt tctcctgcca
    67441 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    67501 ccttgtggct cagagttaaa cggcaagctc aagacctctt gatacacggt gttcaatcag
    67561 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt
    67621 tctctaatac tcaggtcaaa acgctgattt cacaacctct gtttttcatt gtccatagga
    67681 ttgatattgt ccttgtgatg actcattcgt aataattgag gcttgatttt cttcacttcc
    67741 cgttatcgtt tgtccactgc cagaatctga aggacttgtt cctgtcgact cgtttttttg
    67801 agaagagaca gttgttttct tgacttgttc atttgtcttt ttccattccg ctaaacgatc
    67861 ggcgaccact tttttgtcta ctttttcaga ataagcggtg atcacttttc catttttcaa
    67921 acgggctact ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa
    67981 ttgatattgt ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc
    68041 ttcttttcct tttggcaaat agatgtccaa ttcgcctttt tcgtaattgg cagttatttt
    68101 atactccgcc caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct
    68161 tgctacgatt ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc
    68221 tgcggataaa ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt
    68281 tttcctcatg ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat
    68341 gaaatagaaa gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa
    68401 atggtaagaa tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat
    68461 ccgtttttat ctgatacgtc tttgaaccag tgacccgatt ttttgattgt tctttttcca
    68521 tcttgtgcta agtctactga aataaagcca tatcgattct tatatgcatt ggtccatgac
    68581 cagttatcca tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaattgct
    68641 tgatggacat atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg
    68701 ccatcttctt tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag
    68761 cagtcgatat ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca
    68821 tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa
    68881 taatcctcag gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg
    68941 cgtggctggt aataattgat acctaaaaga tctactgtat tttctttgat gaccgtcaaa
    69001 tcctctggat ggatcacagg catgagatca agttctttta ctatctcaat caattcactt
    69061 ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt
    69121 tgcgctgctt taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc
    69181 aagatgatac caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct
    69241 gaggccaacg cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga
    69301 taatgccatc gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac
    69361 cattttttta ctttcgaacc gaacaattca aaacaacttt tggcatattt ttcataggct
    69421 tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa
    69481 tgatacagat tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga
    69541 tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa gcggctccat
    69601 tggatagatg ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga
    69661 tacttcgtat aaacttgtga agttttttca ggtcctactt ggtcaaagaa cttttgcggt
    69721 tcttttttgt accaataatc aaaaatgttc tctccttttc catcacctga aatccttcct
    69781 tctgtttgtg ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt
    69841 ttcatcaaag cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt
    69901 ttccatcaca attacttttt cattttcttc ctcttctttt ttgattcgtt tcaacagctg
    69961 ttctgcggct tgttccccga ttttatctgt tggtggtgta atcgtgattt tcggaaggat
    70021 gctcaaatcg tttttatcca aataatcata atgactaaac gcaatatcct tcccgattct
    70081 gataccttct tgattgaaac attcgatagc accgatcgac atattataat tgctgacaaa
    70141 aatcgctgtt actccttttt ctaataattc ttttgcccca taatagccac tttttctaga
    70201 atagtctcct gtaaagacct ctaattggat cgatggatca ttttgtaccg cttcttttgc
    70261 tcctgctaaa cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt
    70321 tttatgtcct tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac
    70381 taatattgta tcaatacctg ctggtccttc tgggcaattc aatgcgataa ctggaatact
    70441 catttttgtg agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc
    70501 atcgatcaca tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc
    70561 aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga tccctgagac
    70621 aacttccgac ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg
    70681 attattcttc aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc
    70741 tgctattttt ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt
    70801 tcccactgaa acaccagcca tcttagccac gtcacggatc gatgccatac attctcactc
    70861 ctgccagtta catttgattt ctctttcttg ttgttcgatt atgatgaccg ctttctttgc
    70921 atagtgttgc ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct
    70981 cactattaat tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt
    71041 taaaagattg caacactttt ttaaaacgct ttcctaaaat ggcgaatttt ttgccttttt
    71101 aaaattttat tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc
    71161 cgtttgaagt tttactttcc aatggtataa tttctttatc ctatcaaatg ggggaagtat
    71221 tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg
    71281 taatttgttt ggcggctctg accaatcctc ggatgaaaca accgattctt ctgtcttaaa
    71341 agcggaaaaa gcagatatca ctaacagtgt atatacgcta aaagtacgta aagatatgga
    71401 tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca
    71461 gacacaagaa actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagacatcaa
    71521 gattactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaagcaac
    71581 catcacattt accaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag
    71641 tctatagtgc cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa
    71701 gaagcaagat gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat
    71761 gtaataacaa caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg
    71821 acaactgtca caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa
    71881 attatgtaaa aagtagaaga agataaacaa atccaagcaa aagtgactat ggctggtttc
    71941 cagaaacttc gactcagctt ctttctttga tcaaatttca aaaactagaa gcagtaaaac
    72001 aagaagtgga tcatttcagt tcacaaatca aagagtaaag atttatttat ttcaaatatt
    72061 tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt aatttttctt
    72121 tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg ctgattatcc
    72181 gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg gatctgacgg
    72241 atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc atccacttgt
    72301 attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt caacgtttcg
    72361 taataggtat ataacgtttc atcgctttct agctgttcat agtcacgccc acgttttttg
    72421 atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc aggccgtttt
    72481 tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc gtactgctgt
    72541 acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag gctatcttca
    72601 tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat tgctaaaaat
    72661 cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt tgaataaaag
    72721 agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc aatctctttt
    72781 gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat caaaatcact
    72841 gacatacatc ccttcctctt ctataagtac tcgcctacgt aagtttcaac aggcaatgtt
    72901 tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag actttgtgta
    72961 atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt tttcaacata
    73021 gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc aaagtcaagg
    73081 atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta tacttcctgc
    73141 gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg agagtagaaa
    73201 cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg atcgatttgc
    73261 tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc tccttcaata
    73321 ataactgatt tttaatagat actgctcgta attcattgac cagttccaat ggattgttgg
    73381 aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca aaagtttcta
    73441 ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt ggcttgtcag
    73501 gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat gtacctagac
    73561 ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt ctttcatcga
    73621 tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt tcaaaaagcc
    73681 cattcggata gatacctgtg acttttccgt tattttccag tactgtttta ctaatcactc
    73741 ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt
    73801 ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg gaaccgcaaa
    73861 aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc attctacacg
    73921 tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata agatagacac
    73981 cactatatat agtatttaga aaaaaatcat ttttcagcta ttttatagat aaaacaaaaa
    74041 taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact gggatttcgt
    74101 cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt ttattaacga
    74161 aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc taaataaaaa
    74221 agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc tcctaaaaat
    74281 ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg tcactgctgg
    74341 aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt ttgatattgg
    74401 gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact gtataataac
    74461 tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct tcgacataag
    74521 gtatacgaga ccttttcgac aacgaaaggt tctcattttt ccttcatcgt catttcaagg
    74581 agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt atattcgatt
    74641 ctttctaact ctgattcttt cataccattc ctgcctcaag gtacatctat tgtacttttt
    74701 tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga tatgtcttct
    74761 tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt caataataaa
    74821 atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc ccatggtctg
    74881 gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc gccccaaaac
    74941 atgcttaatc cgccacctac tgccccaccg actcccgttg ctatgacatt gcgtgccaag
    75001 tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat aggaaaagcg
    75061 gtcttcaatg tatcgatttc ttgacgattg aatatatttt tacgaatgat tttccctaag
    75121 aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa tacagtgatt
    75181 ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata aatggtaatc
    75241 cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat gagaagctat
    75301 ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttttttta
    75361 tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc gcctccgaat
    75421 attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg ccgatctgtg
    75481 cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag aaatcatcag
    75541 aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat tctttgccaa
    75601 attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa taaatataag
    75661 gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg aaaaaggaaa
    75721 acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct ttccggactt
    75781 catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat cgattgtaag
    75841 gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat tcatctgatg
    75901 ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt tcttctaatt
    75961 cactttctgt cggtaacatc gaaccgactg gatattttcc tgagaaaata tcttctttaa
    76021 ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca ccctttgctt
    76081 tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc acatgtgaga
    76141 agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg aggcaattac
    76201 ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat ttccttcatc
    76261 gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg atgcactgac
    76321 aagatccata catccccaag gcgtatagcc catcaaatct acaccgtctg ctactgcatt
    76381 agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa taatcgtgcc
    76441 atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata atggtttctg
    76501 gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga tctgccagcc
    76561 ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc cttctgaagt
    76621 tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa atccgatgta
    76681 gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta atttttccgg
    76741 atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc cacgtacttg
    76801 gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg cctgattgac
    76861 tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga tttggatatc
    76921 cgaacgcaat tcgtggccga tttttacagc tttgctactt gcaacgaatt gattatgcca
    76981 tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc cttgacttaa
    77041 agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt tgactttact
    77101 gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca gttttcgatt
    77161 tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag aaatcgtgat
    77221 cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa attctaatcc
    77281 cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg caatcgaaaa
    77341 acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga aacgatcgta
    77401 atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg tccagtcaaa
    77461 ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat ctgcaacgga
    77521 tagccctttt ccgtctcgat cataagcccc ttctaattga ttggcagcag ttgctccacc
    77581 ccataaaaat ttttctgaaa aacgtgattg catcctgatt tcctccttat tttacaattg
    77641 ccagaacatc ttcgtttgca ataatctctt tttggtctaa ttcaagtata tcggcaaatg
    77701 catcagtatt cgtaatgaca actggagtga ttacgctata gccagctgct ctaattgctt
    77761 cgatgtcaaa acgaatcaat aaatctcctg cttggacgag atccccttgt ttgactaaga
    77821 tctcaaagcc attcccattt aattctactg tatccatccc gatatgaatc aaaatatccg
    77881 ctccgtcagt cgttgtgatc cctacagcat gtccagttgg aaaaatagtc gtgatctctc
    77941 cgtctgcagg tgcaactagt tctcctactt ctggatcaat agcgatacct tttcccatca
    78001 cgccagaaga aaaaacttgg tctggtactt cactcaatgg cagtacttta cccgttaaag
    78061 gactttttaa ggtatgccgt gctgaagtaa tcggttctcc tgcattcgca tgtttgtttt
    78121 ctaattgatt gtcttgtgtt tgttcgtcaa atcctaaaat caatgtacct acgaatgcaa
    78181 gaacaaaagc aattcctgta gctattactg ctactgtcac atttgactct acaccatcca
    78241 ccgtactaat gaaagtcgga atcgtcaata agctgaccaa actgctggag aaagctttta
    78301 ctccggaaaa tccgataata gctccaccaa tacctccaga aatacaagcg gcaatgaatg
    78361 gtttcttcaa aggtaatgtc acaccataca cagttggttc agtgattcca aataacgaag
    78421 tcactgtaga agaaatccct aatgctcgaa gtttcgtatc ttttgttcgc aatgctacag
    78481 ctaaagcagc cccaccttgt gccaagatgg ctggaagaag cattggcatc aatacatcaa
    78541 acccatattg ttcgatattc aaaaacatga tcggtacgaa tccccaatgc atgccgaaca
    78601 tcacgaatac ttgccataaa cttcccatga ttagacctgc tacgattgga ctaaatccat
    78661 aaatcgagtt gaagagaccg cctaaagcat taccggcaac cgtcccgatt ggtcctaaag
    78721 caagaaatgt taatggaacc atcactacta aaacgacaag cggaactaaa atcatttgca
    78781 gaaattgcgg aatcactttt ttttacgaat ggctctaatt tactttgaac ccatacggct
    78841 agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac tgggatccca
    78901 aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata cacaagtgcc
    78961 atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc tgtaaaagca
    79021 agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat ttgataagta
    79081 ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc tttcaatacc
    79141 ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat atcgatcaga
    79201 cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag accttcctca
    79261 tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg atactgaccg
    79321 ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt atctgctaaa
    79381 gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact tttgacattc
    79441 ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc cattgttttt
    79501 tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca
    79561 ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa tccttatgat
    79621 tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata aatctgctct
    79681 gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg ttcgacacaa
    79741 tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc ttgctcgtca
    79801 gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat gatacgctga
    79861 taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat actcagtata
    79921 tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta
    79981 ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac acccgtcttt
    80041 tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata aaattttttt
    80101 gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt gatcgcataa
    80161 tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt tagtttatct
    80221 tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat atcagccatc
    80281 agctgctgga attggttttt agcaaaagaa tctcttaaaa caaattcttt ttggaccgct
    80341 tctagcgaaa tctcgtcccc aatctttttt ttgaaaagct agtccttttc ccatacaaac
    80401 gacttcttcc ccgaatccat ttttcgaaat gacgacattg ttattcaaaa ttttatcaat
    80461 tttcattttg cttcgccctc tcaagaaaaa taaaaaaacc tagaattcgc ctactgtttc
    80521 ttagctgaat tctggttttg cctgccgtac agtaacaatc catttcttat tcaaaatcat
    80581 cataccatcg aatttttatt tatgcaagcc ttttcatata aaaaataaaa aaactggaac
    80641 acaagtctat ctagaaaaca accaaatcag agatgcacct ctttgcgttc ctcttttttt
    80701 cagcaaattg gtttttcctt atcgattcat atcctttggc tgtttttaat ctgtaacgac
    80761 ttatgtccca gtatcctaaa atgtttttgt ctctagattg aaagcagatc tcatatcatc
    80821 tgtcgcatct tttatcggtg taacactttc cagttctcct tgtacaacta gtcgcgtgac
    80881 ggcgtccatg tcgccacaag taattagtgt gacaatttct ttttcctcga cttcattcag
    80941 taattgggta tctgtaggag caactttttc tttaaaaaat gttttgtatg tatacacatt
    81001 ttctaaatct gtcaaataga tcttggcacc catctctaaa ttttccagcg gtgtaaacaa
    81061 caattcaggg ttcgttgcac gatggcttgc taaggcataa tttccttttc ccatttcttg
    81121 atctggagat aatgttcctg ctccgtaaag tagtgacgta ttatccaagc ctttgaaaat
    81181 cggtaaattg attgaaactg aagggactgc gatactagca atcactggta gatctgttcc
    81241 tctcatctgc gacctcatga ccccttctgt cgtcattggt tctaccgcat caaagtcaaa
    81301 actgacatct ttatcgttat tctttttcag atcttctttt gttacattcg ctatagcata
    81361 tttatctcct gtctctctga caagataatc cttgatctgt tcattgaaaa tcaaagctag
    81421 tcctactaat aacaagagga acaagaggat attgactagc catctttttg cttttccttt
    81481 ttttttcatt ttctctccct ccagactcat tttactgttt ttcttttgct ctttccaaca
    81541 gccgaatgac acatttctaa aactctgtgt atttttttcg tacacctgcg cttttttcta
    81601 caggatattt caaattattt ttgatcaatt tttcttcaat gatctcatct agatccagct
    81661 gaaggttgtc tgccatcatg tatgaataga tcaatacatc cgccagttct tctttgattc
    81721 gttctaattg tgtttctttt acttcttccg gcgttttcca ttgaaaaagt tctaacaatt
    81781 cagatgcttc caaagaaata gaaatagcca aatctttttc gttatgaaat tgtcgccaat
    81841 tacgttcatc tctaaactgg ttaacttttt ccatgctatt catttgcttg ccttcttcca
    81901 atcatttctt acttgattaa tcataccatt tttttacata aaaaatagat gatgctttta
    81961 aaaaaactcc tgttttcttt tatttaaaat aaatgtcata gaaatcagta atttaggtag
    82021 ccaccatgtg tatgcagtaa tgtgacttct tcccctgagg ctttttgttt ttcgatacgt
    82081 tcttctcccc aaacagacat cgctactaga atctctctta atgattttcc ttcttcggtg
    82141 agcgagtact ctactttagg cgggacttgg ttatagactt ttcgatggac aatgccatct
    82201 tgttctaact ctcttaactg ctgggtcagc attttttgtg tgatcgtagg gattttcttg
    82261 cttctccacg cctaaaataa gcgatattgc tcttcccttt atctggtgta agcaaagcag
    82321 ttagcatttt gacgatcgtg ggttccgctt ttcacttctt atctacttag tatcatgaag
    82381 tatactttcg tactaatact aagtagcgga agtgtcaact actttgttta actacatagc
    82441 aaaagaaaca gaagggtgac aacggtcatc acgcttctgt ttcttttgaa tatgctgtgt
    82501 tgttgtctct atctccgtgt agactttcct caagcttatc ctttgacagt aagtcagtag
    82561 gctgatttgc tctttctaga tgccagatac atttctacaa aaaaatagtg attctgtttt
    82621 gaaaattcga tttttagttc tgcattttcg taaacatcca acattttttt cgcactgtat
    82681 agacctaacc cgccattttt tttagaggtt tttcctcttt tgaccatctc cgatagcgtc
    82741 aaattcgtcg gatttgcctt gttcgctata ctaaaacgta atccctcttt ttcttccagc
    82801 agattgatcg taatcggact ctgatcggct acacgatgat cgaatgcgtt agaaataaaa
    82861 atagataaac agcgaataaa aacaaccaga tcaatcggga ttgttttcac ttcgacagga
    82921 ataactactt ggatcgggat tttctccgat tctgcttttt ctagaaattt taaaaagaca
    82981 cccctgacag ctggggatac aatatttgtt agctgttgta tttgcggttt ttcaaaaata
    83041 gctgcagaat gatcttctaa gatttctaaa aattccaacg tttctttttt attttcctga
    83101 agagtaattt ttaatgaaaa cagtagactt ttataatcat gtttgaattc ctctagatga
    83161 tggtaatatt cttcatttat ttgatacagt tgaagcgatt cctgatgttg tctttctttt
    83221 acttggaaac tgatcaacat aatggaaaaa aacaaagaaa aaaaactgat gactatcgac
    83281 aaaagagata gaagcagata attagatgag cggaagtgaa cacttcccaa taccaagtaa
    83341 atcacgcccc agcttaataa aatacaaata cctccaaaat agtgttcagt tggataagta
    83401 aaaagcaagt tgagaaatct cgtctgtttg tctatacttt ttactaatag atagaaaata
    83461 aatgcgataa gtagtgcaaa aaaacttgct gttccaagat gtctctttcc taaataatcc
    83521 atcagatcaa aaaccagaaa aaagctgagt ccagtgatag caaaaaaaag attactgatc
    83581 aaacagctga taaacaggtt tttcgtagta aaataaaaaa gaaacatcaa taataagcca
    83641 actaggtaag gaaagctcac tttagggata ggaaataaaa gtgtcaccac catcaaagct
    83701 atacaaccaa agctgttttt tactagcagt tgtttcgaaa agaataaata gatacctaaa
    83761 tctaaaatca aagtaaaggt caaataaagt gtcactgttg cgaccatctt attcctccat
    83821 tgttttgttt caaaatgtac acatattttt ttaataggag cctgcatatg aaaaagtatt
    83881 ttatcataga agataaccag tttcacagcg aatggatcat caaagaattg gtcaatcatc
    83941 atatcgagaa tatcgtgcag ctcacttcta tctctcaaat caatcagttg atccagttgg
    84001 gtactattga taatcacgat gttttcttta tcgatatcga cttgaagcaa tcactcacag
    84061 gtattgatgt cgccgaacaa atacgtaaaa aaaatgcaca tagtaatatt gtttttttca
    84121 ccgcttatac gaattttgca gtagagatca tcaatcggaa aatccttccg attggatata
    84181 tcagaaaaga caggaattta tctgaacaaa ttcgtcaagt gctagaacag atcaattgga
    84241 aagaacagca aatcgtaaat gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg
    84301 tcctgtatcc tcgggaaatt ttatatattg aaagtatcaa aggtttgaaa aaccaagtta
    84361 tggtcaaaac aactacagaa gataaattag tgaatacttc tttaaaacaa ttgaagacag
    84421 agttggaaaa gtatgcttac tttttactat taaaatctta ctgtatcaac ttatcgcaat
    84481 tgcagaaaat tgattctgct cattatgtgt tagaattttt caatggtgac tccttactcg
    84541 ttggtcgaaa gatctttgaa aagacaaagg aacgtttcca tgacttccaa aaaactgctt
    84601 cgagttgatg aataagagct gagacgtaag catagcagat ttcagggagt tggccttgaa
    84661 tcggctccac tccctgaagt cattgatttg attttacgct ttcccttctg taggagcatc
    84721 tgttaacgac catgtgaatg ttgaaacgta gttgtctacg gcataattgg atacaggaac
    84781 aaataattcc actgattgtc tcccttgttc cacattctct cctaatgaat aaatccatgt
    84841 gccaatccct tcatttgctc ctgcagttac tagatctact gcctgatctg ctgggatctc
    84901 aattccttca gcgccagtac cagaaatagt agatggcgtt ccactgctat tagttgcgct
    84961 atttcgaatc acggcgtttt tgaatttcac ctttgaacca gttagtttat tcgtatcaga
    85021 tgttgtacca ataaattctg ttcgtttgac tgatagtttc caaccggcat aaattccgct
    85081 tttatccgtt acttgtacga aattctgtac ttgtgttgct gtccctgtac tgtccgttcc
    85141 gttttgtaat gcagcatata ctgtttttgc tgtacctaac ttgattgttc caaaatcaag
    85201 atctggagct tggtcaatcg tcagtaatcc ggatgttccc tgatttggtt cgttaggtgt
    85261 aaacggcgta tcgggatcat ttggatcaac aggaggaact ggaacaacgt cttcaggatt
    85321 tgcggcatct ggttttgtta cagaaatcgt tccttctgta gtggctgagc tcttattcta
    85381 agcaaaaaca ctttgtgtac ataataatag acttccagct agaacggtac ttaatgcaat
    85441 tgtttttttc atttcttttt ccttctttct tttaaaataa ataatccagc aaataagcca
    85501 ataagaatga ctccaacaag tatcagccat cgccagtcaa atgtttctgg ctgatttttt
    85561 aaatcaatat ccttttcatt caattggtcc gctgcatcat cagttatttc aaaatcggta
    85621 tggagattcc agcgtttttc tttccaagca cccttcacaa ccgcttgata atgtcccgga
    85681 gctaattttt ttccttctaa tgggacaagt aaatcaaaat ttgtattggg tgctacatct
    85741 attttttctt tttttattga aagataaggc tcttttgttt cttttttgta gatttcaacc
    85801 gatgtagtca agccgaacat ataagaagca tttggatttt gaagattcgc cttaatcaca
    85861 tttcttccat cgctttgatc cgcataagct ttttttaatt tcaaatttgg ggaagactct
    85921 ttttcgctat ttcgaatcaa gattgctttc acttgagaaa actgattgac gacaccaact
    85981 gtacttttgt tagaaacgtt ctcttttttt tcttgaaacg tgattcctcc tgcgaccaca
    86041 ccagccagct ctttttctgg agctttcagt gaaagtgtct cagtgaccgc actttgagga
    86101 gggacagtta tttctcccac tgtttttgct aagtttttca tatcataact taatgtttca
    86161 tcaaatttac tttttgattc tccatattcg atcacaccgt ttgaattcgt cgtagcggag
    86221 tggatcaatg tctcaactgt tactgattta tctgttcggt taaagaccgt gtaggggatt
    86281 tcctgtgttt tcccaggctc catccttaca tttatatatg ttttgttctt atcaatctga
    86341 ttagccggta attcaaaatc taccgcaaaa ttgaattcgg aggcatggac aggtgtctgc
    86401 caaaggatga atccaaggag aaggatcaga agtaaagagc agttgtttcg atgactttta
    86461 ttccatattc gattcatcaa tcattctcct tgatctttat tctgtcggcg catctgctaa
    86521 tgtccatgtc atctctgctg agtaattacc agctatctga ttttctttgg ttaagacaag
    86581 tttaaacgcc cgaccattat ttaaatattc tgaataatct gtttgcacat tcgttgaatt
    86641 ttgtcctctg acaatttcca gttcttcttc attcactaat tgatactcct cagctttctt
    86701 gaaatatagg acatcctgca tagaaaatcc tgcatcggtt gtgaaaggct gagataattt
    86761 tatcgttaag atccatggac tttttgtttc tcgctgatca taaatccata atttttcctg
    86821 tatatccatt tggccaaatg cttcttgcgt caacccagtt gctttgatct gaccacaatc
    86881 gagtttggaa ggggcaagaa atgagagacc accttctact acaatcttcg tagcaggtgc
    86941 aaaaggcggg gcatcatgat aaacaactgg cgtatcactt ggattgtgat tcccaatttc
    87001 atcgttggta cttggcttgt tctgcacacc cttagcagca gcttctcctg cttggtcgtt
    87061 taatacaatt tctaatacat ctcctgcaaa aagttgattt tctgggatag aaagaacaaa
    87121 tttacctgta tcatcaatag ttgtttcgga caaaacagct tgattttttt tcaatgaaac
    87181 caaagctcct gcttcgcctg tacctgtgat tgagcgagaa gctgcattga tcaatgtggt
    87241 acttaattta gcaggctcag gaggaatcac atcgactgtc gttttggcag agtccagttc
    87301 tgtcacttct tggccgcctc ttactttttt agcagcaact atttgcactt gatcccccgt
    87361 tgttagtctt tcttcgttgg gtacttgaac ctcaaacaaa ccgccttgtt cgccttctcc
    87421 ccacggatta tatcctgtcc cttcattttc atctgtttgt ccttgtgtga gagcagtaat
    87481 ttctacaggt gatttcgtcg tatctgcata agtaatacgt aaagtcactt ctacttcgcc
    87541 tgtccaagca tctcttggct ttgcctcgac accttcggga acaacgacat gcccatagat
    87601 ttttttatct gcattcgttg gtactcttaa ggattcaatc atcggaagct ggttattggc
    87661 actgattcga ttataatttt tcattccggc acctgtctga gcaaattcat ttgttaaacc
    87721 tgcactaggg cttgatacaa aattacctaa gtcggttcca ttgatcgata agtctgtcat
    87781 atcagaaaaa taatccggat ctttttcaaa gttatcgaac gttactcctg cctttcgcca
    87841 taaagcaatt tctgaatgag cgatcttcaa agaagaatcg ccatttcctt gaaataccca
    87901 gccacctatg ccattagtct cgcttccgat ccgtttgttt tggaaatcga aataggtagg
    87961 agattctaag tctacattca acacatcccc actgatgatg ccagtcttat cttccccagt
    88021 tttcccgacc atgactaaat aagttttttc gccagccata aagttcaaat caatatctcc
    88081 agttgtatct acggtagctc cggaatctgc gcgtatatcg atatttgatt tttctccagt
    88141 aaccgtaaaa tcagctgacc ctggactttc tagaaaacca gaagcgtcat aaaattggat
    88201 cgcttggttt ccttgattga gtctgttcac ataagatttg ccatcccctg catttcttac
    88261 cttcaatgag cctccattag ttactgttag tttttgcgta ccgttctcaa aacgaactgc
    88321 gctagaacga ccactttgct tatcaatatc gatgactgat ccggctcctt ctacgatcaa
    88381 agaaagtcct ttccctacaa aacgcatccc attgtttcca ggttggtcgc catctccagc
    88441 cattttgacc gagatttgtc caccacttga tacatggatt tgactagaat ctccttgcag
    88501 gcgaaatccg ccagtagtcg ctgtattgat tgtgattttc ccatcattcg acacagcgac
    88561 tcttgatcct gagcctctca tttggataat tccgcgattg ttgtcgttgg taatattgat
    88621 ctttgtattt accgaaaatt cacttcctga gccactgact ttaaatgtga cattcccggg
    88681 tagcgtatcg acagcctctc cttcatcgct gacgccatct gcccgatcat agtaaagaga
    88741 gacctttgaa ccactcaaga tatggagtcc tggtgctgtt tttccactgc ccgtctcatc
    88801 acctgaatct agaaaaccat agaattttcc tagatatggt ccataaagct gactaccatt
    88861 gattacattt agctgatagc ttctacaaaa ataagcttta cccggagcgg cattcatccc
    88921 ttctttcact ggttggacat ccaatacacc ggaaacacca tctaatgtga cagttgcacg
    88981 aggggcgtaa atcaggccta atttagaacc atcatataag tcaagtgtcc cggtaaattt
    89041 tatttctccc gtaaaatgat atctgtttcc ccaaaaatcg gacatataag caatgataat
    89101 ggcatttcca tcgatatttg gtatcaaccc acctggatga gctgccgttc ctttaaaatt
    89161 ctgaaactcg atcgactgag gtgtctcatt gatatagatg tgatgtgtgc cgaaattatg
    89221 agcaagtccg ccaaaatcga taacgatatc tcgcttgatc tccaattccc tgtctaaagt
    89281 caaaggctga gttaaacgaa tcttagctac tgattcgtct gcgagaagct gcgtcagttc
    89341 ttctgttgtg gcagcttcgc tgtacccatc cgctgaaaca actctaacct gtacctcttc
    89401 actgctatca gacgtttgtt cagctacttc tgaggtctca cttgtcttat tttcttcttg
    89461 ccttgtatta gaagtaggcg cctcactttg ggattctgta gtttcagaag taattgattc
    89521 ttctgaaaaa gtagtcttgt caaaactttg attctctgat tgcgttgatt cgcttgtttt
    89581 ctctacatcg gcaaaaacgg atgtcaccat caagagaggg aagacaaaca aaatcgacgc
    89641 aatcagcttt tttacatatt gattcattct ccaccatcct ctctgacgtt ttgacaattt
    89701 tattttaacg ttttaattta tttgtaattg tactttcata tttaa
//
LOCUS       DAPBAB010000006        81883 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPBAB010000006 DAPBAB010000000
VERSION     DAPBAB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 81883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.006"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..906
                     /locus_tag="RD444_000541"
     CDS             <1..906
                     /locus_tag="RD444_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7547934.1"
                     /translation="IENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDST
                     THEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNP
                     FWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCK
                     ICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYN
                     DAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTT
                     GEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            1004..1192
                     /locus_tag="RD444_000542"
     CDS             1004..1192
                     /locus_tag="RD444_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDT7547935.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1250..1603)
                     /locus_tag="RD444_000543"
     CDS             complement(1250..1603)
                     /locus_tag="RD444_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7547936.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2134..2556
                     /locus_tag="RD444_000544"
     CDS             2134..2556
                     /locus_tag="RD444_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7547937.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2553..2783
                     /locus_tag="RD444_000545"
     CDS             2553..2783
                     /locus_tag="RD444_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7547938.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3281..3481
                     /locus_tag="RD444_000546"
     CDS             3281..3481
                     /locus_tag="RD444_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7547939.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3508..4701
                     /locus_tag="RD444_000547"
     CDS             3508..4701
                     /locus_tag="RD444_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7547940.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4769..6334)
                     /gene="guaA"
                     /locus_tag="RD444_000548"
     CDS             complement(4769..6334)
                     /gene="guaA"
                     /locus_tag="RD444_000548"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDT7547941.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6393..7316)
                     /locus_tag="RD444_000549"
     CDS             complement(6393..7316)
                     /locus_tag="RD444_000549"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDT7547942.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7512..8117
                     /locus_tag="RD444_000550"
     CDS             7512..8117
                     /locus_tag="RD444_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7547943.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8425..9441
                     /locus_tag="RD444_000551"
     CDS             8425..9441
                     /locus_tag="RD444_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7547944.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9582..10883
                     /locus_tag="RD444_000552"
     CDS             9582..10883
                     /locus_tag="RD444_000552"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDT7547945.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10929..11591
                     /gene="deoC"
                     /locus_tag="RD444_000553"
     CDS             10929..11591
                     /gene="deoC"
                     /locus_tag="RD444_000553"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDT7547946.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11612..12010
                     /locus_tag="RD444_000554"
     CDS             11612..12010
                     /locus_tag="RD444_000554"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDT7547947.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12076..13158
                     /locus_tag="RD444_000555"
     CDS             12076..13158
                     /locus_tag="RD444_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7547948.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13370..14932
                     /locus_tag="RD444_000556"
     CDS             13370..14932
                     /locus_tag="RD444_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7547949.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14925..16058
                     /locus_tag="RD444_000557"
     CDS             14925..16058
                     /locus_tag="RD444_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547950.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16058..17014
                     /locus_tag="RD444_000558"
     CDS             16058..17014
                     /locus_tag="RD444_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7547951.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17113..18279
                     /gene="deoB"
                     /locus_tag="RD444_000559"
     CDS             17113..18279
                     /gene="deoB"
                     /locus_tag="RD444_000559"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDT7547952.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18303..19121
                     /locus_tag="RD444_000560"
     CDS             18303..19121
                     /locus_tag="RD444_000560"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7547953.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19138..19845
                     /gene="deoD"
                     /locus_tag="RD444_000561"
     CDS             19138..19845
                     /gene="deoD"
                     /locus_tag="RD444_000561"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7547954.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(20143..20829)
                     /gene="gpmA"
                     /locus_tag="RD444_000562"
     CDS             complement(20143..20829)
                     /gene="gpmA"
                     /locus_tag="RD444_000562"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDT7547955.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21001..21582)
                     /locus_tag="RD444_000563"
     CDS             complement(21001..21582)
                     /locus_tag="RD444_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7547956.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21715..22398)
                     /gene="rpiA"
                     /locus_tag="RD444_000564"
     CDS             complement(21715..22398)
                     /gene="rpiA"
                     /locus_tag="RD444_000564"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDT7547957.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22717..23130
                     /gene="rpsL"
                     /locus_tag="RD444_000565"
     CDS             22717..23130
                     /gene="rpsL"
                     /locus_tag="RD444_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDT7547958.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            23214..23684
                     /gene="rpsG"
                     /locus_tag="RD444_000566"
     CDS             23214..23684
                     /gene="rpsG"
                     /locus_tag="RD444_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDT7547959.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23778..25865
                     /gene="fusA"
                     /locus_tag="RD444_000567"
     CDS             23778..25865
                     /gene="fusA"
                     /locus_tag="RD444_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7547960.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            26037..27224
                     /gene="tuf"
                     /locus_tag="RD444_000568"
     CDS             26037..27224
                     /gene="tuf"
                     /locus_tag="RD444_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDT7547961.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27631..27939
                     /gene="rpsJ"
                     /locus_tag="RD444_000569"
     CDS             27631..27939
                     /gene="rpsJ"
                     /locus_tag="RD444_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDT7547962.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27970..28599
                     /gene="rplC"
                     /locus_tag="RD444_000570"
     CDS             27970..28599
                     /gene="rplC"
                     /locus_tag="RD444_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDT7547963.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28627..29250
                     /gene="rplD"
                     /locus_tag="RD444_000571"
     CDS             28627..29250
                     /gene="rplD"
                     /locus_tag="RD444_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDT7547964.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            29250..29540
                     /gene="rplW"
                     /locus_tag="RD444_000572"
     CDS             29250..29540
                     /gene="rplW"
                     /locus_tag="RD444_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDT7547965.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29579..30412
                     /gene="rplB"
                     /locus_tag="RD444_000573"
     CDS             29579..30412
                     /gene="rplB"
                     /locus_tag="RD444_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDT7547966.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30455..30733
                     /gene="rpsS"
                     /locus_tag="RD444_000574"
     CDS             30455..30733
                     /gene="rpsS"
                     /locus_tag="RD444_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDT7547967.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30756..31103
                     /gene="rplV"
                     /locus_tag="RD444_000575"
     CDS             30756..31103
                     /gene="rplV"
                     /locus_tag="RD444_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDT7547968.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            31117..31773
                     /gene="rpsC"
                     /locus_tag="RD444_000576"
     CDS             31117..31773
                     /gene="rpsC"
                     /locus_tag="RD444_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDT7547969.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31776..32210
                     /gene="rplP"
                     /locus_tag="RD444_000577"
     CDS             31776..32210
                     /gene="rplP"
                     /locus_tag="RD444_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDT7547970.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            32200..32388
                     /gene="rpmC"
                     /locus_tag="RD444_000578"
     CDS             32200..32388
                     /gene="rpmC"
                     /locus_tag="RD444_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDT7547971.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            32413..32679
                     /gene="rpsQ"
                     /locus_tag="RD444_000579"
     CDS             32413..32679
                     /gene="rpsQ"
                     /locus_tag="RD444_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDT7547972.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32737..33105
                     /gene="rplN"
                     /locus_tag="RD444_000580"
     CDS             32737..33105
                     /gene="rplN"
                     /locus_tag="RD444_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDT7547973.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            33142..33450
                     /gene="rplX"
                     /locus_tag="RD444_000581"
     CDS             33142..33450
                     /gene="rplX"
                     /locus_tag="RD444_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDT7547974.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33478..34017
                     /gene="rplE"
                     /locus_tag="RD444_000582"
     CDS             33478..34017
                     /gene="rplE"
                     /locus_tag="RD444_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDT7547975.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            34036..34221
                     /locus_tag="RD444_000583"
     CDS             34036..34221
                     /locus_tag="RD444_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDT7547976.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            34257..34655
                     /gene="rpsH"
                     /locus_tag="RD444_000584"
     CDS             34257..34655
                     /gene="rpsH"
                     /locus_tag="RD444_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDT7547977.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34687..35223
                     /gene="rplF"
                     /locus_tag="RD444_000585"
     CDS             34687..35223
                     /gene="rplF"
                     /locus_tag="RD444_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDT7547978.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            35384..35740
                     /gene="rplR"
                     /locus_tag="RD444_000586"
     CDS             35384..35740
                     /gene="rplR"
                     /locus_tag="RD444_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDT7547979.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35761..36261
                     /gene="rpsE"
                     /locus_tag="RD444_000587"
     CDS             35761..36261
                     /gene="rpsE"
                     /locus_tag="RD444_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDT7547980.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            36276..36455
                     /gene="rpmD"
                     /locus_tag="RD444_000588"
     CDS             36276..36455
                     /gene="rpmD"
                     /locus_tag="RD444_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDT7547981.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36499..36939
                     /gene="rplO"
                     /locus_tag="RD444_000589"
     CDS             36499..36939
                     /gene="rplO"
                     /locus_tag="RD444_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDT7547982.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36939..38234
                     /gene="secY"
                     /locus_tag="RD444_000590"
     CDS             36939..38234
                     /gene="secY"
                     /locus_tag="RD444_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDT7547983.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            38296..38943
                     /locus_tag="RD444_000591"
     CDS             38296..38943
                     /locus_tag="RD444_000591"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDT7547984.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            39134..39352
                     /gene="infA"
                     /locus_tag="RD444_000592"
     CDS             39134..39352
                     /gene="infA"
                     /locus_tag="RD444_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDT7547985.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            39385..39501
                     /gene="rpmJ"
                     /locus_tag="RD444_000593"
     CDS             39385..39501
                     /gene="rpmJ"
                     /locus_tag="RD444_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDT7547986.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39519..39884
                     /gene="rpsM"
                     /locus_tag="RD444_000594"
     CDS             39519..39884
                     /gene="rpsM"
                     /locus_tag="RD444_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDT7547987.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39912..40301
                     /gene="rpsK"
                     /locus_tag="RD444_000595"
     CDS             39912..40301
                     /gene="rpsK"
                     /locus_tag="RD444_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDT7547988.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40381..41319
                     /locus_tag="RD444_000596"
     CDS             40381..41319
                     /locus_tag="RD444_000596"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDT7547989.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            41347..41727
                     /gene="rplQ"
                     /locus_tag="RD444_000597"
     CDS             41347..41727
                     /gene="rplQ"
                     /locus_tag="RD444_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDT7547990.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41999..42838
                     /locus_tag="RD444_000598"
     CDS             41999..42838
                     /locus_tag="RD444_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7547991.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42814..43683
                     /locus_tag="RD444_000599"
     CDS             42814..43683
                     /locus_tag="RD444_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7547992.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43673..44470
                     /locus_tag="RD444_000600"
     CDS             43673..44470
                     /locus_tag="RD444_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDT7547993.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44673..45419
                     /gene="truA"
                     /locus_tag="RD444_000601"
     CDS             44673..45419
                     /gene="truA"
                     /locus_tag="RD444_000601"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDT7547994.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45752..48421)
                     /locus_tag="RD444_000602"
     CDS             complement(45752..48421)
                     /locus_tag="RD444_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDT7547995.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(48659..48847)
                     /locus_tag="RD444_000603"
     CDS             complement(48659..48847)
                     /locus_tag="RD444_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDT7547996.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            49117..49479
                     /locus_tag="RD444_000604"
     CDS             49117..49479
                     /locus_tag="RD444_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7547997.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            49531..51213
                     /locus_tag="RD444_000605"
     CDS             49531..51213
                     /locus_tag="RD444_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDT7547998.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            51330..53366
                     /gene="recG"
                     /locus_tag="RD444_000606"
     CDS             51330..53366
                     /gene="recG"
                     /locus_tag="RD444_000606"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDT7547999.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            53415..54416
                     /gene="plsX"
                     /locus_tag="RD444_000607"
     CDS             53415..54416
                     /gene="plsX"
                     /locus_tag="RD444_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDT7548000.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            54538..54780
                     /gene="acpP"
                     /locus_tag="RD444_000608"
     CDS             54538..54780
                     /gene="acpP"
                     /locus_tag="RD444_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDT7548001.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            55048..56052
                     /locus_tag="RD444_000609"
     CDS             55048..56052
                     /locus_tag="RD444_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7548002.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            56053..56997
                     /locus_tag="RD444_000610"
     CDS             56053..56997
                     /locus_tag="RD444_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7548003.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            56997..57959
                     /locus_tag="RD444_000611"
     CDS             56997..57959
                     /locus_tag="RD444_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548004.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            57986..58900
                     /locus_tag="RD444_000612"
     CDS             57986..58900
                     /locus_tag="RD444_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548005.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            58926..60707
                     /locus_tag="RD444_000613"
     CDS             58926..60707
                     /locus_tag="RD444_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7548006.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            61055..61741
                     /locus_tag="RD444_000614"
     CDS             61055..61741
                     /locus_tag="RD444_000614"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDT7548007.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            61761..65342
                     /gene="smc"
                     /locus_tag="RD444_000615"
     CDS             61761..65342
                     /gene="smc"
                     /locus_tag="RD444_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDT7548008.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            65351..66160
                     /locus_tag="RD444_000616"
     CDS             65351..66160
                     /locus_tag="RD444_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7548009.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            66171..67169
                     /gene="ftsY"
                     /locus_tag="RD444_000617"
     CDS             66171..67169
                     /gene="ftsY"
                     /locus_tag="RD444_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDT7548010.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            67356..69623
                     /gene="gshAB"
                     /locus_tag="RD444_000618"
     CDS             67356..69623
                     /gene="gshAB"
                     /locus_tag="RD444_000618"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDT7548011.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(69712..70164)
                     /locus_tag="RD444_000619"
     CDS             complement(69712..70164)
                     /locus_tag="RD444_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDT7548012.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            70314..71264
                     /locus_tag="RD444_000620"
     CDS             70314..71264
                     /locus_tag="RD444_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDT7548013.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            71257..72216
                     /locus_tag="RD444_000621"
     CDS             71257..72216
                     /locus_tag="RD444_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDT7548014.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            72213..72968
                     /locus_tag="RD444_000622"
     CDS             72213..72968
                     /locus_tag="RD444_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7548015.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            72991..73944
                     /locus_tag="RD444_000623"
     CDS             72991..73944
                     /locus_tag="RD444_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7548016.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(74588..74884)
                     /locus_tag="RD444_000624"
     CDS             complement(74588..74884)
                     /locus_tag="RD444_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDT7548017.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            75239..75595
                     /locus_tag="RD444_000625"
     CDS             75239..75595
                     /locus_tag="RD444_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7548018.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            75605..76504
                     /locus_tag="RD444_000626"
     CDS             75605..76504
                     /locus_tag="RD444_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDT7548019.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            77021..77752
                     /locus_tag="RD444_000627"
     CDS             77021..77752
                     /locus_tag="RD444_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7548020.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            77897..79924
                     /locus_tag="RD444_000628"
     CDS             77897..79924
                     /locus_tag="RD444_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDT7548021.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            80032..81129
                     /locus_tag="RD444_000629"
     CDS             80032..81129
                     /locus_tag="RD444_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDT7548022.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            81436..>81883
                     /locus_tag="RD444_000630"
     CDS             81436..>81883
                     /locus_tag="RD444_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548023.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        26833 a        14680 c        17494 g        22876 t
ORIGIN      
        1 attgaaaatc cgcaccctct actacaaaca actgttgaac cgagtaaacc tgaacagaga
       61 gaaatgttgc ttgatgccct tttggaaatc tcagatagtg atccgcttct acgatattac
      121 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
      181 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
      241 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
      301 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
      361 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
      421 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
      481 atctgtttta agtacggttt atactatagc cctgttagta ctccagcaga ttttcggatg
      541 cttactccta ttgtactgga gcaagccttt agaaaagctg gaacagaatt gttagagcca
      601 tatcttagtt ttaaagttta tgcaccacag gaatatcttt cacgggcata taacgatgct
      661 cccaaatatt gtgcaaatat cgtaaatact caactgaaaa ataatgaggt cattattatt
      721 ggagaaattc ctgctcgatg tattcaagat tatcgcaatg atttaacttt ttttacaaat
      781 gggcttagtg tttgtttagc agagctaaaa ggatatcagg ttaccactgg cgaacctgtt
      841 tgccagaccc gtcgtctaaa tagtcggata gataaagtaa gatatatgtt caataaaata
      901 acttagtgcg ttttatgttg ttatataaat atggtttctt attaaataag atgaaatatt
      961 ctttaatata gatttgaatt aaagtggaaa ggaggagatt gttattataa actacaagtg
     1021 gatattgtgt cctatttgtg gaaataaaac aagactacga atacgagtgg atactatact
     1081 taaaaatttc cctttatact gccccaaatg taagaacgaa actttaatta atgttcaaaa
     1141 aatgaatata ataacaatca aagagccaga cgccaagacg cagagccgat aatttgagaa
     1201 atgaaactct catcttatcg gctctttttg tttatctgaa ttttactgac tagccttcaa
     1261 tatttcttgc ttcattgata ccttttgcta gtgcttccat taaggatagt tctttgtctg
     1321 taaagctatc catgtatttt tctacctgta accttcgagt gcttttaatc aaattatctg
     1381 taggtaagaa aaattcatca atagatacat ggagtaatga tacaagatca tacagcactt
     1441 gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa ataacgtggg tcaatttcaa
     1501 tcattgttcc cacttgttca cgagtgagac cccgtttcat tcgagcctct ttaatcgcta
     1561 aaccaacggc tctaaaatcg tatttatctt cttttttacg catactaaat cacctctata
     1621 cattttactg ttcctatata accataaaca ggtatagaaa aacatgttat atagtttatg
     1681 agttcctata gaacactgtg gaatttatta atgcgatgaa tccaatttca aatagaaagg
     1741 tcgctgattt tatttaccta tacaacttta taaaatttag tcatactcct agaaagtaga
     1801 taaaattggc gacttaatgg agggaaaagt cgccatgaga aagataatct gtcatctagt
     1861 tctcttaatt aagttcttat caaaaaaact tatccacctg tactggatag atatgaccat
     1921 caatgtggtt ttgcagatta catagataga aaaatcaaag catatgaaca gagaaaacaa
     1981 tctgtttatg tgcttttttg gtcacttaat aaatatcttt taggatttct cttcatttat
     2041 gcaacaaatc ccccgttcgt gtcggggttt agtatgaagg gagataaaga agcacatgcc
     2101 actccctttc acgacagaaa gggggtgaaa agcatgaaac catcttcttt tcaagaaacc
     2161 atagaaaatc agtttgacta tatctgcaaa caagttattg aagatgaacg aaaagattac
     2221 ttcaaacatc tagacagact atcagcaaaa gaagtctcgt tttctgatat agataactac
     2281 cttgtcaact ctttttctac tgttgatacg tacgtcactg atttccaact atttaaatta
     2341 tttggattaa gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg tcatctttca
     2401 gagaaaaaac ggaacattat tttacttcat tactttatgg atatgagtga tgtggagatt
     2461 gcggagttac taaatttgaa tcgttcgact gtttatcgtc atcgaataag tggactagcc
     2521 atgattaaag aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca agggttccct
     2581 tccccctcat tgtaaaggcg acagatggcg acgtggaagc aattaatcag attgttaaac
     2641 attatcgagg gtatacctca aaacgttcat tacgtcgcat gacagacgaa tatggaaatt
     2701 ctcatatggt tattgatgaa accctacgtg gcagaatgga aacgaaactc atctctaaga
     2761 ttttatcgtt cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca
     2821 cgcttcccga tttgggaggt ttgttattcc atcaaagcat attgtgtgtt cagtaggttt
     2881 tgataggcta acaagccaaa cgttctttga caaccaaata aaggcaatcg aatacgtttc
     2941 ggtaagaaaa gagctgatga actaacacgc catgacctac aaaattagtg agcgattcat
     3001 gttagtaatt cgaaagtaat ctttagcagg attgctgcca tgactttctt atcgtgataa
     3061 tgatactccc gtatagccat agtccgagcg ttagaagcgt cacaggcaat gggtacggct
     3121 acatgagaac catgcagaag tgaaattcct gtgagctttg ctagagctgt tcgattgcta
     3181 agatattctt ctcttgtcag tgtattatta taaaactatt tgatataatt ataggtgaat
     3241 attggagttt tcctagccta taattgggaa aggagtaata atgataacgg tcacaaaaaa
     3301 ggatttagtt gagctaggat atggaacttc tttttcagca gacatcataa aaaaagcaaa
     3361 agaattgatg attacaaaag ggcatatcta ctatcaatct cgaaagctag atagagtccc
     3421 tcgtgaagct gttgaagaaa ttctaggaat tcggttttca aatgaaaaca ggtctgagta
     3481 gtttttgcac agtaaggagt aaaaactatg gttaaagacc caataaaaaa agcaaagaat
     3541 ggaacctatt attttcgtgc aaatctaggt tatgattcta aagggaaaaa aattcaaaaa
     3601 tatcgtagtg gattcactac taaaaaagaa gcaagagaag aatattcgaa actgctattg
     3661 atgaaaccag aagaattaag tgagaataag gataaaatga cgtttaaaca ttatatcttt
     3721 gaaattttcc ttccatggta taagacacag gtaaagctca gaacatatga aaatcgctta
     3781 ccaactatta agaagcattt ctcttatttt gataaaatgg ctgtttccga tattgagcct
     3841 attgatgtgc aaaattggca actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga
     3901 gctgttcaag gactattttc agttgctatg gatcgagcaa ttgtccttgg gataaccacc
     3961 acaaatccat ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg
     4021 acaaaagaag aatttgaaaa agtaatctct ctaatttaca aagaagatta ctatcaacac
     4081 tttttattta tctctctgtg gtttttattt atgactggaa tgcgaattgg tgaagctact
     4141 gccattcaat gggaagatat tgattttgac tcaggagtac taaccatcaa caaaacactc
     4201 tattacaaga atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt
     4261 catattgtat tagataaatg cacattaact tatttatccg aatggaagga agtacaacaa
     4321 aatctaattc agacagactt tgtaatgagc tataacggta ttcctaccca aaaacataca
     4381 ctatcatatg ctattgaacg ctatgctaag ctggctggta tccatcgaat cagaatccat
     4441 gggttacggc attctcatgc ttccttgtta atcagtatgg gagaaaatcc acttatcatt
     4501 aaagatcgtc taggacacga agatattgag acgactttag gaacgtatgg acatctttat
     4561 cctaacagta atttcgaggt agctaacaaa ttaaatggag ccatttcctt taaagaagca
     4621 aaacaaaatt atgatacttc accaaagaat caatttactg tagactactt gcgaaacaaa
     4681 gaaatgaaaa aagtgcaata aaagtgcagt aaaaagataa gaagagccag aaacccttgg
     4741 tataagagga tttctagctc tcctacaact attcccactc cactgttgct ggtggtttag
     4801 aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata cgtacagaga
     4861 ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg tcgattgaag
     4921 ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata acaccgacag
     4981 agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt ccagcagctg
     5041 cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg tcttcagtga
     5101 tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc caaacgattg
     5161 aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat aatgtgttta
     5221 atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta tggtgagatt
     5281 tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac gttccttgtg
     5341 ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca aaaacatata
     5401 cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct ttcaacttgt
     5461 ctaagaagcg atctcttgca tcgactttga tgatgttcaa gccgaatttt ccgcctagac
     5521 tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca aagatagatg
     5581 tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa tcaacgccac
     5641 ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt tctacttgca
     5701 tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca aaggcaaagt
     5761 ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat tggataccat
     5821 aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt gttccgactg
     5881 tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat actgtttgtt
     5941 gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac aaggctttcc
     6001 catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg atcaattgca
     6061 taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga tcgatcccaa
     6121 atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc tttggattca
     6181 tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag actccaagat
     6241 cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat acgataatct
     6301 tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc cctttaaatt
     6361 ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg
     6421 atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt aggcaagata
     6481 tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat tttaaaggct
     6541 tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg gaaagccgta
     6601 tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg atcggcatcg
     6661 acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc aggtaattga
     6721 agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc gtacttgtct
     6781 ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc ctgaccagat
     6841 tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg aaatccttta
     6901 cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc gtcagtcgtg
     6961 atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag cagtctggct
     7021 gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg cggtacttct
     7081 acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc tttcatataa
     7141 agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat ggaaatgcgg
     7201 tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg cccattacga
     7261 taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc attcatatta
     7321 gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc aaaccccacc
     7381 acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa acggagtctt
     7441 attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa gaaatgagaa
     7501 ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg cacgattttg
     7561 aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat tctggtgtat
     7621 tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc gattgggaag
     7681 agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg attgggttag
     7741 ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa cgagcggtaa
     7801 gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc catcaatcga
     7861 atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat ccgccgatcc
     7921 gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg ttgaagaagg
     7981 gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg caaaagaaaa
     8041 tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat tatatcatca
     8101 aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg gaactgggcg
     8161 tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa acaaagaact
     8221 attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga taaaataaat
     8281 aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt ttttatcaaa
     8341 aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac tgatagggaa
     8401 tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc agaaatggca
     8461 ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag atttagtgat
     8521 aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc cgattatttc
     8581 gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc tgatgtcact
     8641 gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc tttgggctat
     8701 gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta tttacaagag
     8761 ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt acctgagaaa
     8821 tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga tcgcggattg
     8881 aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta tcaagctgtt
     8941 tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac cactggatac
     9001 tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca tggcctgcct
     9061 tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata tactgcatca
     9121 aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga tgccatggcg
     9181 atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc
     9241 ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac gacagtgcag
     9301 cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac gatcgaattt
     9361 cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg tgagagcgta
     9421 aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt tacaaaggct
     9481 agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac ctttcctaaa
     9541 aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg gtggatctga
     9601 ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac atcgtcacga
     9661 actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg gcaatttttt
     9721 atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct aattctggtg
     9781 atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg acaggtggcg
     9841 ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga gtgacagtgg
     9901 ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag ctagaagcaa
     9961 ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt aatgaaagta
    10021 aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct
    10081 tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg attatgagta
    10141 aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga gacggtgctt
    10201 tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc attgggaaac
    10261 tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt ggcgaagcta
    10321 tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag
    10381 atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct ggcaaagcta
    10441 agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt aaagcgttag
    10501 caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg gaacatccag
    10561 aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc ggagttgtgt
    10621 caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa
    10681 ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa attggtgaca
    10741 ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa atcacttctg
    10801 tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga
    10861 ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta aagaaaaggg
    10921 gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca gacgcatcta
    10981 aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat tctgtctgca
    11041 tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca gtagccgtat
    11101 gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca tttgaaacaa
    11161 cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc ggtgctttga
    11221 aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag
    11281 atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa gaaatcatcc
    11341 gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca actggtttct
    11401 caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa
    11461 tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca atgatcgaag
    11521 ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt tcaactggtg
    11581 aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag aatggatcaa
    11641 cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc cggtaggcgc
    11701 ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga atgcatcatt
    11761 tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa
    11821 aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc cttgtggtgc
    11881 atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc tagtaggaga
    11941 caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa
    12001 agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac aattatttta
    12061 ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt cgcactagca
    12121 gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc cggtggcaaa
    12181 agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt agacgaccgt
    12241 tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca aggactttca
    12301 cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta catcccaaat
    12361 attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta taaactaaaa
    12421 ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat tatcgatgat
    12481 gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga agcttcttat
    12541 cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt tatcggcggt
    12601 gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt tgatgcaggt
    12661 gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga cttctctgct
    12721 ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc
    12781 catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt gaacgaatca
    12841 ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga aggcgaatac
    12901 acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc agttggaaca
    12961 gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg cgaacacact
    13021 gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc tgacgaagcg
    13081 aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa
    13141 actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt tgacaaggct
    13201 atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac agtttggata
    13261 agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat
    13321 cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga
    13381 aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca aggcaaatga
    13441 caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag aaaacggtgc
    13501 tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat cagggaaaat
    13561 tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc aattagggat
    13621 tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag aaaacatcgt
    13681 gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac gaaaagagat
    13741 ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg ttcgtgatat
    13801 ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag gagcagatgt
    13861 actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg aattaatcgt
    13921 gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc ataagttaga
    13981 tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag gaatcggtac
    14041 agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg gaagaacggt
    14101 ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa
    14161 tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact tagaggttcg
    14221 tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg agttgatcca
    14281 agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag gggaagacat
    14341 cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc cagaagaccg
    14401 tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac tacaaaccta
    14461 tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga atgaacatgc
    14521 cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc
    14581 tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga
    14641 tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg aatttattca
    14701 taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca gtttcgaatt
    14761 agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag aaattgtcgg
    14821 tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg cgggctatac
    14881 cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat gatcgcagcg
    14941 aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg atcttaggcg
    15001 cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct atgctagatg
    15061 cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg ccgttgattt
    15121 ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt gggctttctg
    15181 gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt ccagatctgc
    15241 caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt gcgatggcag
    15301 cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc gtaacaatca
    15361 tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg atgcctgaaa
    15421 gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca ctgcggttac
    15481 catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt ttagcaatca
    15541 tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt gaaatccgtt
    15601 ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg
    15661 ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt gaaggattag
    15721 gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt gacggaatgg
    15781 ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg atcttgttca
    15841 gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg tttgagatcg
    15901 tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg attcgtttcg
    15961 tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca atagaatcga
    16021 aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg aattaattgc
    16081 cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt cactaggcgg
    16141 aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca tggtcatggg
    16201 tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt ctatgacacc
    16261 atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga ttcacgcagt
    16321 agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta atctgatggc
    16381 acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa ctgataacat
    16441 cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg tcattggacc
    16501 aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg ctgtcctagc
    16561 ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc
    16621 tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag gcgtactgat
    16681 ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt ccggtaactt
    16741 ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga tctttggtaa
    16801 gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc aaagcttgag
    16861 tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc tgcaaattgc
    16921 tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt ctggtccaaa
    16981 agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa aagggtgggt
    17041 tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa
    17101 gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt aggaattgga
    17161 gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg acacattgcg
    17221 aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac gatccgtcct
    17281 cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt agaagaagtt
    17341 tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa tatcaaaact
    17401 ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat cgaagaattt
    17461 tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat tgacgattac
    17521 ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga tcctgtcctt
    17581 cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat ttgccaattc
    17641 gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc acgtccgtac
    17701 gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc gttgaatcca
    17761 ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga
    17821 aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac gaaaagcaat
    17881 atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg aatcagcttt
    17941 accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt cggctatgcg
    18001 catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat ggaagaagac
    18061 gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg taccgatcat
    18121 acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca aggggcattg
    18181 cctcaaggat attattcaga tatttcagca acgattgctg agaactttag tgtttcttcg
    18241 actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat aggagaaagc
    18301 aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt gataaaggag
    18361 taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta gcagatgaaa
    18421 tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt tcgacagtag
    18481 ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg ttagccatgc
    18541 aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg
    18601 tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg
    18661 aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt actggtgaaa
    18721 atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat atgagtcaag
    18781 cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta ggtttggatc
    18841 ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct gcggagatcc
    18901 gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca gaagtcatcg
    18961 tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac ttagcagcag
    19021 gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga gtcaaacaat
    19081 cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa caataaaatg
    19141 agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact tccgggagat
    19201 cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg ttacaatcag
    19261 gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc tgttcaagga
    19321 acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca gtcgtatggt
    19381 gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt
    19441 gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa
    19501 gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata tgagatagct
    19561 aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc tttctacaaa
    19621 gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt ggaaatggaa
    19681 gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag tttgatgacc
    19741 gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca atctacgttc
    19801 aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg aaatgaaaaa
    19861 gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat gtttttgact
    19921 tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
    19981 tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt catgtttgat
    20041 gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg
    20101 actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt agtatttttt
    20161 agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg gaatttcaag
    20221 atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc gtaatgagtt
    20281 accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa tttcatcttg
    20341 ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag gaatatcgcg
    20401 tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag tttcttccat
    20461 taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt cgccgtattt
    20521 ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt cgtttaagcg
    20581 ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca agttacatgt
    20641 tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc cagcttcttt
    20701 gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat ttacatcagc
    20761 ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag aaaaaactaa
    20821 ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt ttcagttctg
    20881 ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag attctatgag
    20941 aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg tttttaggta
    21001 ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat aacatgttcc
    21061 aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt cataatagag
    21121 acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga tttttccttt
    21181 tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc actccatgtt
    21241 tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat cgattccttc
    21301 atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg
    21361 ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc cttgttgcat
    21421 gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca cttcatatgt
    21481 ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag ttgttaaatc
    21541 aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc ctttctagca
    21601 gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca aaaagatagt
    21661 tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt ttctttattt
    21721 acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta ctttagaaac
    21781 catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat aggtagctaa
    21841 gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac caccatctgt
    21901 ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt tttcttcaaa
    21961 aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg ggaacttccc
    22021 aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg agtacgtagc
    22081 gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa aatcagcact
    22141 gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa tactctttaa
    22201 cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat
    22261 acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat aggcggttga
    22321 accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt caatgccagc
    22381 taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc gtgctttcct
    22441 cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc aataaagctt
    22501 tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat tctaacaaag
    22561 gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga
    22621 taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg
    22681 aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa ccaattagta
    22741 cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa caaaggatat
    22801 aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg ggtagcaact
    22861 cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt
    22921 cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca taacttacaa
    22981 gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg ggtacgttac
    23041 catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca aagccgttct
    23101 aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg aaacttgctt
    23161 ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta cggatgccac
    23221 gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac tcaaaattag
    23281 taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca gctaacatta
    23341 tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta gaagtgtttg
    23401 aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt gttgggggtt
    23461 caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt
    23521 gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag
    23581 aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa gacactcata
    23641 aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct ctctgtttgg
    23701 tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag atagtaaaga
    23761 gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt aatatcggta
    23821 tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg tactatactg
    23881 gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac tggatggaac
    23941 aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa tggaagggtc
    24001 accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa gttgaacgtt
    24061 cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt gttgaacctc
    24121 aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc gtatttgcaa
    24181 acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta catgaccgtt
    24241 tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac ttcacaggaa
    24301 ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga acagagatcg
    24361 aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc gaaaaattgg
    24421 ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa ggtgaagaaa
    24481 tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta gaattctacc
    24541 cagtactatg tggttcagca tttaagaaca aaggggttca attactattg gacgctgttc
    24601 ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac cctaaaactg
    24661 atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta gcatttaaag
    24721 ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac
    24781 aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc
    24841 tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga gatatcgctg
    24901 ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa aagagtctag
    24961 ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt gaacctaaat
    25021 caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa gaagatccta
    25081 ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt atgggtgaac
    25141 ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag
    25201 gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt
    25261 ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa tttacaccaa
    25321 atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg
    25381 aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt gtcttagctg
    25441 gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat gatgtcgatt
    25501 caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca gctaagaaag
    25561 ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct gaagattatt
    25621 taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt atggaagctc
    25681 gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg tttggttatg
    25741 ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca tttgaccact
    25801 acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg
    25861 aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac tatattttta
    25921 atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc ttgtgtacat
    25981 catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat tttaaaatgg
    26041 caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc ggacacgttg
    26101 accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag aaaaacggcg
    26161 gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa cgcggaatca
    26221 caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct cacgttgact
    26281 gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa atggacggag
    26341 ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa cacatcctat
    26401 tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta gacatggttg
    26461 atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta acagaatacg
    26521 aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct ctagaaggcg
    26581 acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa tacatcccaa
    26641 ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg ttctcaatta
    26701 ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg
    26761 aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa
    26821 tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct ttactacgtg
    26881 gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt acaatcacac
    26941 ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt ggacgtcata
    27001 ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac gtaacaggtg
    27061 ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta acaatggaag
    27121 ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc cgtgaaggcg
    27181 gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact taagttttaa
    27241 agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg atctttgttc
    27301 cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata aatagaagaa
    27361 acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa aatgtttttt
    27421 acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg tgcgtttact
    27481 ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg
    27541 acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta
    27601 cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg tatccgttta
    27661 aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga aactgcaaaa
    27721 agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag tctttacaca
    27781 atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat gcgtacacac
    27841 aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta
    27901 gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa aaataatgga
    27961 ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca aatcttcact
    28021 gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt agtactacaa
    28081 gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca agatatgcgt
    28141 gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag
    28201 cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg aaaagaaatt
    28261 acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac gaaaggtaaa
    28321 ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc tcacggttct
    28381 cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac
    28441 aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt ggaagttgta
    28501 cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg agctaaaaaa
    28561 tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga aaggaggaac
    28621 taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg tgaaatcaca
    28681 cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga tgcaatcgta
    28741 atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc
    28801 ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc
    28861 cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg ttcttacagc
    28921 tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt
    28981 gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa
    29041 ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt tctagaaaaa
    29101 ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt agtaacttct
    29161 gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac acaaactgct
    29221 cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc aaacgcccag
    29281 tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt gaagtggaca
    29341 ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac gttaaagtca
    29401 aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat
    29461 atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa gaaatccaat
    29521 tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag ggaaaaacgt
    29581 ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag gttctgattt
    29641 tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga aaaaccatgc
    29701 tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac acaaacgcca
    29761 atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg ttcaaacgat
    29821 cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag atggagtgaa
    29881 agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt caggtccaga
    29941 agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg gtactgttat
    30001 ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg ctggtacaag
    30061 tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact ctggtgaagt
    30121 tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg aacaacacga
    30181 attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc caactgtacg
    30241 tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta aagctcctat
    30301 cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca aaactcgtaa
    30361 caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat aattactttg
    30421 accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa agaaaggacc
    30481 ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa
    30541 agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg gatataccat
    30601 cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca tggtaggaca
    30661 caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg acgataagaa
    30721 aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc acatcagcta
    30781 aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta attgacctta
    30841 tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca aacaaatctg
    30901 ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat aactttgact
    30961 tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct acaatgaaac
    31021 gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca agtcatatca
    31081 cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt acatccaatt
    31141 ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga aaaagagtat
    31201 gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa acttgctgat
    31261 gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac
    31321 acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa
    31381 gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat caaaaaacca
    31441 gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct
    31501 ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc tcaagggatc
    31561 aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga aggatactca
    31621 gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg ggaagaagca
    31681 gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga aattcttcca
    31741 actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac
    31801 accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat
    31861 tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc caaatcgaag
    31921 ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg attaaaattt
    31981 tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct ggtaaagggg
    32041 cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg
    32101 ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa
    32161 ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga
    32221 gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga attgtttaat
    32281 cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa agaagtacgt
    32341 aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt atggaaggag
    32401 gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt
    32461 tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc
    32521 tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa caacacagca
    32581 aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac aaaacgtttc
    32641 cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat tttcctatat
    32701 agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga aagtcgttta
    32761 aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct tggtggatct
    32821 ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca agcaacgcca
    32881 ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga
    32941 gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt gattattcgt
    33001 gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga actacgtgaa
    33061 aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg agacacttat
    33121 caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa agttatcact
    33181 ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt
    33241 attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa
    33301 ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt ggttgactct
    33361 actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct
    33421 aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc ttactgaatg
    33481 aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga aaaatttaac
    33541 tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt
    33601 gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac tttgatcact
    33661 ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa
    33721 ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga attcttagac
    33781 aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag taaaaaagcc
    33841 tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt cccagaagta
    33901 gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac aacagcaaac
    33961 tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca aaaataatta
    34021 aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa
    34081 ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc agtttatcgt
    34141 aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca aattcccggc
    34201 gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg
    34261 tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac atggttaaac
    34321 atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac
    34381 gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc gtgatccgtg
    34441 ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa cgtatctcta
    34501 aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg aacggtttag
    34561 gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa
    34621 atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac aaggaggtgt
    34681 ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt aacagttact
    34741 caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg tactttttca
    34801 gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt
    34861 aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat ggttgtaggt
    34921 gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta
    34981 caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat cacaccacca
    35041 gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg tgcgaacaaa
    35101 gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga accttataaa
    35161 ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa aactggtaaa
    35221 taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt ggctgaagtt
    35281 taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga aacgtattta
    35341 ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta caaaaccaga
    35401 taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct ctggtactgc
    35461 tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc aaattattga
    35521 tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg
    35581 aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa
    35641 aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc
    35701 tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg aataccattc
    35761 atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc aattaaccgt
    35821 gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt
    35881 gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc
    35941 cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt tggttcaaca
    36001 atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa acctgctgta
    36061 gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt agctggggta
    36121 gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca
    36181 gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg cggcaaatca
    36241 gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa gttactttaa
    36301 aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg ttaggtctta
    36361 caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc atgatcaata
    36421 ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata actagaacat
    36481 taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa
    36541 gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc cggacgcgga
    36601 caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga agggggacaa
    36661 acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg taaagactat
    36721 gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt aactcctgtt
    36781 gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat
    36841 ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa
    36901 gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta ttaaaagatg
    36961 cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat
    37021 ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg caaaaccttt
    37081 cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg caaaactttt
    37141 ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt cagcttcttc
    37201 aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa
    37261 aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa tctgtcggta
    37321 tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct aacgtagcaa
    37381 cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact tggctaggag
    37441 aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt gcaggtatca
    37501 tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata aatattgatc
    37561 cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg gtcttgttga
    37621 tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc caatatacga
    37681 aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat gcagctggag
    37741 ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt ttacaagcat
    37801 tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc tttaactata
    37861 atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact ttcttctacg
    37921 cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa ggaagttata
    37981 taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta ctgatgcgtt
    38041 taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc attgctcaaa
    38101 tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg atcgttatcg
    38161 gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt aaatatgttg
    38221 gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct cacacattta
    38281 ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg taaaggaaca
    38341 caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg agatatgttc
    38401 cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta tatggataaa
    38461 ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact agctgaacca
    38521 gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca agcaaaagct
    38581 ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga tatccatgtt
    38641 ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa ttgtggagca
    38701 acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg
    38761 catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg tctggctatc
    38821 aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt gctaaacact
    38881 attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa
    38941 tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag ttcaataaat
    39001 tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca ttttgagaat
    39061 tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa
    39121 ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag tcgtcgaaac
    39181 tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc ttgctactgt
    39241 ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag taacggttga
    39301 attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat aattgtactc
    39361 cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa
    39421 cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc aaatccaaaa
    39481 cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt gcaggagtag
    39541 atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt attggtaaca
    39601 ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc gttcgtgaat
    39661 taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa gttgaagggg
    39721 atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg
    39781 gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta
    39841 ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta agtgaaggag
    39901 gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag
    39961 aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg atcactgata
    40021 ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa
    40081 aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca gcaatggaac
    40141 acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt gaagcagcaa
    40201 ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg actccagttc
    40261 ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga
    40321 gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt aaagataaga
    40381 atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga ttatggcaag
    40441 ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc cctacgtcgt
    40501 attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga tggtgtactg
    40561 catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat cttgaacatt
    40621 aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat cgatattacc
    40681 ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga aattcttaac
    40741 aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg cttaacagtg
    40801 aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat gccaattggc
    40861 gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca agttgagaac
    40921 acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg gacagatggt
    40981 tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga acatttggat
    41041 atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa
    41101 acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc tgttcgctct
    41161 tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa taaatctgag
    41221 cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt taaagcaaaa
    41281 ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg aaggagggaa
    41341 accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc gatgttgcgt
    41401 gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa
    41461 gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga tttgcatgca
    41521 cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag
    41581 gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc tcgttacgca
    41641 gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg tgatggtgca
    41701 ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt atagatgatt
    41761 cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc gctgctcctc
    41821 aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat accttcaatt
    41881 acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag aaataattga
    41941 aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag gagtaatcat
    42001 ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg atcttcgccc
    42061 cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta ttattggaca
    42121 caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc tgccagaatc
    42181 agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga cgatccgaca
    42241 gatggttgga atgatttttc aaaatccaga caatcagttt gttggttcga cagtagaaga
    42301 tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt
    42361 gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt
    42421 atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac gacctgatat
    42481 cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag aagtgatctc
    42541 tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca ctcatgatat
    42601 tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac ttgtccgtga
    42661 aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag gtctagatct
    42721 accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg tgcccagtga
    42781 atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga acaagtagac
    42841 ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat caatatgacg
    42901 atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa atcaacctta
    42961 cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat tggagaacga
    43021 gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa agttggcatc
    43081 gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga cattgctttc
    43141 ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa agaaacatta
    43201 gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact ttcaggaggg
    43261 caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt acttgtattg
    43321 gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga gatgttttgg
    43381 cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat ggatgatgta
    43441 gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa tagcggggag
    43501 ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg cgtacctact
    43561 gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac cttgcctttg
    43621 acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa
    43681 taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat tggatccccg
    43741 agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa ataattggga
    43801 aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa aagtagattt
    43861 gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca ctgtagcttt
    43921 gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt tccatatcac
    43981 tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga tcatttttat
    44041 gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga ttgagtactt
    44101 gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga tgctgtcgat
    44161 tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga acgctcagcg
    44221 cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc
    44281 tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg cgactgctat
    44341 ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat tgcattggca
    44401 taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac tgatcttttt
    44461 aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct gtttgcaaga
    44521 aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta aggtcacagg
    44581 cctctttctt ttatctgaaa agaaagaacg aaaaaatttt atagaaaaat cattattcca
    44641 gataaccttt taggaacaaa ggagagaaaa acatggtacg ttacaaagca atcattgcgt
    44701 acgacggaac aaattttaat ggattccaaa aacagccaaa tgggcgtact gttcaagaag
    44761 aagtagaaaa aacattacaa aaaatggcaa atggaaaaga aatcacggtc tttggttcgg
    44821 gaaggacaga tgccggagta catgcgatcg ggcaagtaat ccattttgat tatccagagg
    44881 agcgtccgtt agaacgaatg cgtttcgcgc tagataccca gtcaccagag gatattgctg
    44941 taagacaagt agaaattgtt tcagaagact tccacgcaag atatttggtc aaagaaaaga
    45001 cgtatcaatt tcgagtggat atagggaaac cacgcagtcc ttttcgacgc cattatgcaa
    45061 gttattttcc ttacccactt gatttggaaa aaattcagcg ggcgcttcct gatttattag
    45121 ggactcacga tttcacctct ttttgtgcat caggaagctc gatagaggat aaaatacgta
    45181 cgatctacga ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg tttacattta
    45241 ggggaaatgg cttcttgtat aagatgatac ggatcttagt aggaacgctt ttgaaaattg
    45301 gcaatggccg attgcctgaa gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg
    45361 cagcaggacc tacagcacac cctgaaggac tttatttgta tgaagtgaaa tatgaataag
    45421 aggttatgat agaagtggtc agcttcaaga aataagaagg tatttacgaa aattacttct
    45481 caaaattttg caaaatttcg gcttattttc gaagaagcta cttctggaac accgtttaag
    45541 aaagaggtcg tgagcctgtc atttaactct gagtcacaaa gagcaatttc aaaaaacagc
    45601 ttctcatctt tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt
    45661 ttgttaccta ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt
    45721 gcggacagtc tgaacaggtt ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt
    45781 ttcgtggttg ttcttctatt gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa
    45841 ggtaccagac ataaagaata aacaatgcaa tacataggac aaaaaggatt gcacctatcg
    45901 taaatccttc aggcaagtaa ctgaaagtga cagtatgttt acctgctgga acttttatca
    45961 tcaagaatgc gtctttgaaa ttgctgacct tgactggttt cccatcaatt ttagctttcc
    46021 atcctttgtc ataaggaata gttgtcacca acatggaatc ttcagcggta tttacagtcc
    46081 cagaagcttt tctgccagtg gcttccattt cgacaccttt ttctttgatc tgattcattg
    46141 cggcttgata ggctacaaca tctagaccaa cgactttagg ctccatgaaa cttatttctt
    46201 tcgtcccata aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa tagccaagat
    46261 catagtattg accagaaatg ttgatttgcg tttttcgcga tgtaccattg acagtgatcg
    46321 tcgcactaga actttctaat tgcgcaaaat tggttgggaa caaacttaag taagcttgtg
    46381 tatttgcagg cacatggact tcccaagtga gctcttttgg ctggttatct gctgtttctg
    46441 agtaagtgac gccagtcgtt gtctttgtga tcgtcgtatt caactgattc ttcaaagtag
    46501 gtgtatagaa tgtaaaatac tgttcattta gaccagacaa tgtattgatc aagtttgtct
    46561 gactggtcaa attatctgtc actggctgct caacatcatt aacggacaac ggtgcagtaa
    46621 atgctagtgg caatgcattg acattctgat acagtgtata gtcgccgctt tgaccagctt
    46681 ggcggaagcc atatttactg aacgtgcttt cgtttttaga aatgttatat ttgattcctg
    46741 taaacccatc catcagcaaa gtattattgt tataacggat attcaaattt gtccctcttg
    46801 aacggaaacc taatttatcc aggtaagagg aagaattacg atttcttata gaagaaaaca
    46861 aactgatacc actgtaacca tagttgatcg aatcgtttgc agatactgga tccagatttt
    46921 ccaagcggta aaacgagtcg gaatcttttt ttgtttcatt tacaagagct ttcaagtcag
    46981 gataaggatc agtatataaa ctgcgggaag cataattcca atcatcaaga atgccatgga
    47041 tcatactgtt cgtattgacc agtgcttcag cagacatgat caataataac aatatactca
    47101 gatgattgaa tgaaagtttt ttatgctgat aaaatcccac agcaaaagca taaatcccta
    47161 agaaaaccac tgtcaataca aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt
    47221 tgatgcccca tgccaccgca aagagagcag ctacgaccaa aatgattcct aacaacagcc
    47281 caccatcgtc ttgttctatc ttttcccatc cttttcctgc aagcaggagc actaagaaag
    47341 agaacagata ggcataacgg aataaaaaca tattcggtgc atgcatacca tgccagaaca
    47401 aattcaatgg aacaagataa aaacttgcga taagtatggc gaaaagactg ccgtacaaaa
    47461 atttattttt caacggtact ttccgactgg caaaataaaa cagacagagg actaatggta
    47521 gcagacctgc gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa acaccaatca
    47581 tatttttcat gattagatcc agataagaag tcgcttctgt tttcaacgta gtgatctgac
    47641 tcagtgcctc gccatttgac cgaagatcca gcactgctgg caatacaatg atcattgaag
    47701 cgccgcctgc cagtaaagaa gtgataccat aaggaacaat cgttttttta tattctgacc
    47761 agtttgtcaa tagacgggcg ataaaatata agaaagaaaa tacgccaatc ataaaaccca
    47821 tataaaaact ggataaaaac aagagtaaat aactgataaa taaaagtttc ggccgtttaa
    47881 gatccataac acgatgaatc cccaaaatca ctaatggaag ataaacaaat gcatccagcc
    47941 acatgatgat ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg acgctaagtg
    48001 caacatgccc ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg
    48061 cacagccgac cttaagcaaa gtcagaacat acagtgcatc tggcatcatc tggtttggaa
    48121 aaaagaacac cagtggcgtg aagatcccgc ctagatagta agaaattaaa gataaataat
    48181 tcaatcctag agaagcattc catgtataaa aaatactttg ctttccaaga agaacatttc
    48241 ggaagctggc atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa atacttctgc
    48301 tgcttccagg atagattccg atacttagat aaacaagtgc taaaatcaaa aacggaatca
    48361 ggaaactagc tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa ttttttttca
    48421 tatttatttt ccaccaaatt tctatgaagt tgtattcagt ttatcatatc cttgtttttc
    48481 tctgcttaaa aagagtctag ttttcacaaa aaaataaaat ttagaataaa taacttcata
    48541 atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag tcccaccctt
    48601 aaaaattttt attaaataaa aaaccgttca ctatctgaac ggtccaagta tcattcaatt
    48661 aaacgcgttc aacttttcct gatttcaaag cacgagttga cacccaaact tttttaggtt
    48721 ttccgtcgac cataacgcga actttttgta agttaggttt aacagtacgt tttgttgagt
    48781 tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg acctgtaaag taacaaactt
    48841 ttgccatggt aatttcctcc tttgccttga tttggagcaa tagaataatt cagctcatac
    48901 cagactaaca tatcatatag aatgggaatt tgcaagtaca ttttcataga tacaattgac
    48961 aattgagaag aataattgta aattaaacaa ggttatacct gaaattttgc aaggtttttg
    49021 agtactttgg gtaactgcct tttatttcaa gttgcgctat gataaaatat gatagaagaa
    49081 aaatgggagt tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat tcaaacgtca
    49141 gctggaacca ttgaaattac caatgatgta attgcaacag ttgttggtgg cgccgcgaca
    49201 gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa tattactgag
    49261 attttgcgta aagaaaatta ctcaagagga gtagttgtcc gccaagaaga taatggtatt
    49321 gctgtagatg tttatacgat tatcagctat ggtacgaaaa tctctgaagt ttcccgcaac
    49381 gtccaagaaa aagtgaaata caatctagag gcattattag gcgtaactgc caattcagtt
    49441 aatgtctttg tacaaggtgt tcgtgtactg ccagactagg caggagactg gctggtaact
    49501 cagcagtcga tctgaaggag gattatctag gtgagtgtaa cagaaataag tgcaagccag
    49561 ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga atacgtcaat
    49621 tcattaaatg tattccctgt gccagatggc gatacgggaa caaatatgaa tctatctatg
    49681 actagtggag ctaaggcagt agtagattca acctcagaaa aagtaggaga attagcagca
    49741 atgctttcaa aaggattgct gatgggtgcg cgcggaaatt ccggcgttat tttgtctcaa
    49801 ttgtttcgtg gattttctaa gcaaattcca gatgttgtct cattaaatgc ttctgatctt
    49861 gctgcagcct tcactcatgg agttgaaact gcgtataaag cagtaatgaa accagtagaa
    49921 ggaacgattt tgacagtagc tagagaagca gcgaaagctg gagaaaagaa agcaaaaagt
    49981 actgatgatg tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg tgctttagct
    50041 aaaactccag acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag tggagggcaa
    50101 ggactgctgt ttgtttatga gggcttttta agtgcattga atggagaatt ccaagcggat
    50161 gaaacatatg aaccttctcc agcagaaatg gatgatatgg tcaatgcaga acatcatcgc
    50221 agtgtccaag gtcaaatggc gacagaagat atccattttg gttactgtac agaaatcatg
    50281 gtaaaaatcg gagaaggacc tacagtcgat agtacattcg attacgaaga attccgtaac
    50341 tatttagatg gtataggaga ttctttactt gtggtcaatg atgacgagat catcaaagtc
    50401 cacgtgcata cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt cggttcatta
    50461 gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga cgattttgga acacgatgaa
    50521 gaagtgaacg agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat cgtagcgatc
    50581 gctgctggta aaggcgtcca agagctgttt gaaagtttag gcgctaatta tgttatcagt
    50641 ggtggacaaa caatgaatcc aagtacggaa gacattgtta aagcgattca agaagtaaac
    50701 gcagataaag tgattgtctt gccaaacaat aagaacatct tcatggcagc tgatcaagca
    50761 gcagaagtag cagatatccc agtagcagtt gttccttcaa gaacgatttc ccaaggattg
    50821 acagctatgc tttcattcaa tgatcaagct tctttagaag aaaacaaaga aacgatgaca
    50881 gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga tacctcaatt
    50941 gacggtgtag agatcaaaga aggagacttt ttgggcatga tcgatggaaa aatcctgatc
    51001 tctaacccag atatcacagc aacagcgtta agtacactag aacaaatgat caatgaggac
    51061 acagagatcg taacgatctt ggcaggtgat gaaggaacag ttgaacaagc aaatgaattt
    51121 gctgagaaac taacagaaaa acatcctgat ttggaaatcg agatccacca aggagatcag
    51181 cctgtctatc cttatctatt ttctgctgaa taatttctgt gtcagaatat ctttctaaca
    51241 cagtattcat tcataaaagg ccttcggtta gtgatcaggt atgatcgctg accaagtcct
    51301 ttttttgtta aaggagggag gaaaaattta tgcgttcttt agatgatcca atcactatgc
    51361 tggcaggagt cggagaaaaa agagcagcta gtcttgccag cttgggcatc caaacgattg
    51421 aagacttact gacttattat ccttttcgct acgaagatat tcaggaaaga aatctaaacg
    51481 agatccagga tcaagaaaag gttactttga aaggaatcgt agtctcgcct cctgtgatga
    51541 atcgatttgg ttataaaaaa agccgattgc aatttcgtat gatgcaagaa catgatgtgt
    51601 tcaacgtttc tttttttaat caaccttatc taaaagaaaa agtcatttta tcggaggaaa
    51661 tcgccgtata cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg aagattttag
    51721 gttctcaaac aatggatgac ttttcgccga tatatcacgt caacaaatca attcgtcaaa
    51781 cgacattagt tgatctaatc aaaaaagggt ttcaagaata cggcacattg attgaagaaa
    51841 atctgccaaa caaccttgtg gagaaatatc gactgcttga ccgagcttct gctgtaagaa
    51901 gtatgcattt tccaaaagac cacgaagaaa accatcaagc caaacgacgt gtcgtttttg
    51961 aagagttctt tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa aaagcagaaa
    52021 cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa acaatttact caaaagctac
    52081 cttttgaact gacaaatgca caaaaacgtg taacgaatga aatctgtcgg gatcttcgca
    52141 gtccgcagca tatgcagcga cttttgcagg gagatgtagg tagcggaaaa acagtggttg
    52201 ccgcgattgc gttatacgct accgttacag caggtttcca aggtgcgttg atggtgccta
    52261 cagaaatctt ggcacaacaa catatggaaa gcttgcaaca gctgtttgat cccaatgaag
    52321 taaccatcgc attgattacg gggtcaacga aagcaaaaga acgacgtgag ctccttgaaa
    52381 aactagagca aggggaaatt aacgtcgtga tcggcacaca tgcattgatc caagatggag
    52441 tcgaatttca gaatttaggt ctagttatta cggatgagca gcaccgtttt ggcgtgaacc
    52501 agcggaaagt acttcgtgaa aaaggctggc gaccagatgt cttatttatg acagcgactc
    52561 cgattcctag aacattagct attacagcat atggtgagat ggatgtatct atcattgatg
    52621 aacttccagc tggacgtatc ccgatcgaaa cacgctgggt aagaacacct caattggaca
    52681 gtgtactgga ttggacgaaa aaagaactgg caagaggaca tcaaatgtat gtcatctgtc
    52741 cgctgattga agaatcagaa gcattagatg taaaaaatgc agtggaaatc tatgaaaaac
    52801 ttcgtgaact atttgcacca aaatatgaag ttggcttgtt acatggtaaa atgaagaatc
    52861 aggaaaaaga agtaatcatg gaagccttca aagacaacca gatgcagata ttagtttcga
    52921 ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg atcatggatg
    52981 cggatcgatt tggattggca cagctgcacc aacttcgtgg acgtgttgga cgcggaagtg
    53041 atgcttccta ctgtatctta gttgctaatc ctaaaaatga actaggtgtg gaacggatga
    53101 aaatcatgac agagaccacc aatggttttg tactaagcga aaaagatctg gaattacgtg
    53161 gtcctggtga agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg attgctgact
    53221 tggtgacgga tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc ctttggcaaa
    53281 taaaggattg gcagatgctt cctgagtatc agcctttagc gcaaagcctc caaatagaaa
    53341 atcaggataa tcgttatttc gattagcaga ctgtgctata ctttatactg atgacggaaa
    53401 aaggagtgga aacaatgaaa attgccgtag atgcaatggg cggagatcac gcacctcaag
    53461 caattgtaga aggcgtggct ttagcgcagc aagattttcc agaagtagaa ttcttacttt
    53521 acggaaaaga agcagaaatc aagaaatact taacaaatga aaaaaacatc acgattatcc
    53581 atacagacga aaaaatcaat agtgatgatg agccagtaaa agcaattcga cgaaaaaaga
    53641 ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat gctgtatttt
    53701 cagcagggaa tacaggcgcg ctactagcag cagggttgtt catcgtagga cgtatcaaaa
    53761 atatcgaacg ccctggcttg atgtctacct tgccagtagt agggaaagaa ggtgccggtt
    53821 tcgatatgct ggatcttggc gctaacgcag aaaacaaacc agaacatttg ttgcaatacg
    53881 gtattttagg ttctttttat gcacgtaaag tccgaggtat cggacgtcct cgtgtcgcac
    53941 tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa gcctatgaac
    54001 tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt gagctactca
    54061 atggtgtggc tgatgtagtg gtcacagatg gatttacagg caacgctgtt ttgaaatcta
    54121 ttgaaggaac agcaatgaat atgatgaatc tattaaaatc tgctattcta aatgaaggga
    54181 tcaaaggaaa aatgggcgct atgcttttaa aagacggctt acgttctttg aaagcagaaa
    54241 tggattattc caaacatggc ggtgccgttt tgtttggctt gaaagcacca gtcattaaaa
    54301 ctcatggagc aacaggcccc gatgctgttc gctatacgat ccgccaaatc cacacaatgc
    54361 ttgagacaga tgtagttgga caattagtcg aacaatttga aaaaagcgaa gaatagtatt
    54421 tggatatctg aagcgatccg ttccattgaa aaaaaggtgg acattcctga aaaatgccag
    54481 taaaattagc catggtgtag gtaattgaca agtgtatgat tcttggaggt gaaaaagttg
    54541 actcgcgaag aagtatttaa caaagtagcg aaaattattt caaatcattt tgagatagat
    54601 accgataaag tgacggatga attgaacatc aaagatgatt taaaagctga ttcaattagt
    54661 attatggaat tcgttttaga gcttgaagat gaattcggta cggaaatttc agatgaagac
    54721 gctgaacaaa ttgaaacagt cggtggcgca gtagattata tcagcagcca tcttaaataa
    54781 tttcaaataa aagcaaagaa ggataaaaag ctgcatgcag ctttttatcc ttcttttttt
    54841 aatctttttg acacgtaaaa gtcatttttc tgataattct ggcgataaac agaaattatg
    54901 ttgcaaagta aagcggtttt ctatataatt attaataatt cattacacaa agataagaat
    54961 aacctttgtt ataaaacgat ttattctgtg taatgtcctt ttttattaag aaaaacttcc
    55021 atttaggaag cagagagggg tattcgattg tccgataacc aattattaga agttcagaac
    55081 ctacatacag gattccgact caaagatgac tattacgatg cagtagatga cgtttcattt
    55141 actttagaca aaaacgagat tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc
    55201 ttggctacta cgattatggg attacttgat cctaataaca caaaaattac aggtgagatt
    55261 ttatataacg atctgaattt aatcgacctg aacgaaacgc tctataataa aatccgcgga
    55321 aatgatatcg gaatgatttt ccaagatccg ttgtctgcat taaatcctct catgagaatc
    55381 gaagaccaga tcaaagaagg actttcctat cacacaaaga tgaatgcaga acagcgtcaa
    55441 gctcgtgcac tagaattgct agaacaagta gggattccta atcctgcgag agttggacgc
    55501 caatatcccc atgaactatc aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc
    55561 gcctgcaaac ctccaatcgt cattgcagat gaaccgacga cagccctaga tgtaaccatc
    55621 caagcacaga ttcttgattt actgaaagac cttcaggaag aaacaaaaac agggatcatc
    55681 ttgatcactc atgacttagg agtcgtagca gaaatggctg atcgagtggc agtgatgtat
    55741 ggcggacagt tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc taagcatcct
    55801 tatacacgct ctttactgaa ctctatacca caagaaggaa atcatgatgc agaactccat
    55861 gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca
    55921 ccacgtattc catggatacc agcaagcgat catgaagaag agcctatcct tcatgaagtc
    55981 gcaccgaacc acttggtacg ttgctcctgc tataagcatt tccactttag agacgaaaaa
    56041 ggagaggtgt agatgacaga attaatcaca atcaaagacc tgaaagttca ttaccctatt
    56101 cgcagtggat tcttcaatcg agtaacggat catgtatatg ctgtagatgg tgtggatttt
    56161 attatcgaaa aaggaaagac atatggttta gtgggagagt ctggctctgg aaaatctact
    56221 acgggaaaag ccgttgtggg attagaaaag gtaacagccg gacaaatcat gtatgaagga
    56281 aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg
    56341 atcttccaag attcaatgtc cagtttgaat cctaaaaagc gagtgttgga tatcattgct
    56401 gaacccatcc gaaattttga acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga
    56461 ctgctagata tcgttgggat gccggaagat gcgctatata aatatcctca tgaattttca
    56521 ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc
    56581 gtagccgatg aacctgtatc tgctctggat ctatctgttc aagcgcaagt attgaatttc
    56641 atgaaacgaa tccagcaaga atacggcctc agctatttat tcatttcaca tgatttaggt
    56701 gtagtaaaac acatgtgcga caatatcgca attatgtaca aaggtcgatt tgtagaaatc
    56761 ggtacaagag aagacattta taatgacccg cgacatatct atacgaaacg tctgctttca
    56821 gctattccgc aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg acgaagagta
    56881 gaacaagagt atatccaaaa ccaaaaagaa tactatgatg cgactggtcg agtctacgat
    56941 ttgcgtacga tcacaactac acacaaagtg gcgttgaaag atggaggtgc aagctaatgt
    57001 ggaaaacgat tctgcgacgt gttttattga tgatcccgca agttattatt ttaagtgttc
    57061 tgatcttctt attagcccaa gcgatgccgg gggatccatt taccggtttg atcaatccaa
    57121 atcaggatcc ggcagtgatc gaacagatgc gacaagcagc tggcttgaat gacccatggt
    57181 atgtccagta cttccgttgg atcggaaatg ctttgcaagg cgattttggt caaagtttct
    57241 tatacaaacg ctctgttgct gacttgatcg gcgaacggat catcaacact ttatatctat
    57301 cgatcttgac agttgtcctt acttatttga ttgctgtgcc tctggggatg cttgctggcc
    57361 ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta caacttcttt agttttgctg
    57421 ttcctttatt tatcttcggc ttgatcatgc tatttgtttt tggttatcgt ttaggctggt
    57481 tcccaacaag tggatcacaa acactaggtt tgcaaggtgg atttatcact caatggatag
    57541 accgtttgaa atatattctg ttaccatcaa ttacccaagc atttttggcg actgccgtca
    57601 caatccaata tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt gtcagaacag
    57661 cccgttccaa aggggtaccg acgaataaag tattcagtcg acatattttc cgaaatgcag
    57721 cgttgccgat ggcttctcaa atggggtatg aaatcacttc gttgattgct ggttctgtag
    57781 ttatcgaaaa gatttttggt tatccgggag tcggaaaact atttattgat tccatcggcc
    57841 aacgggacta taccgtgatc acatccttag tattgatttt aggaatcgcg acgctagtgg
    57901 ggacattact ttcagatatc atcatgagta tcgttgaccc gcgtattcgg attcaataaa
    57961 accaatcaga aaggagaatg gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag
    58021 tattcctcct atggggatac gaatgattgc tcgagaattt aaaaaagata aaattgctat
    58081 tttttcatta ggtttgctag tggttttatt attagtgatc tttatcggtg cgatgctgac
    58141 cgatcaagac aaagtgatgt atgttagtat cttcgacaaa tacgctgcac caggaacagt
    58201 ttcgacacaa ggacaaaagt tcattctggg agcagatgaa ggcggacgtg atgtattagg
    58261 acaattgatc ataggtgcta gaaactctgt tacgatcgga tttgcgatca cgatccttac
    58321 ttcgatcatc ggggttggat tagggatcat ctctggctac tacggaggga tcattgacaa
    58381 catcttgatg cggatcgttg attttatcat gattctaccg atcatgctga tcattatcgt
    58441 cttcgtatcg attatttcca gatacaatgt ctggtccttt atattcatca tgagtgcatt
    58501 ttattgggta gctaaagccc gattattccg aagcaagaca ttatctgaag caagaagaga
    58561 ttatgtcagt gcctcaaaaa caatgggaac caatgatttt aaaatcatgt tccgcgaaat
    58621 catgccgaat ctaagttcat taatcattac taatttgacg atgaactttg cggcaaacat
    58681 cggtatcgag acaactttga ctttcttagg tttcggcttg ccggcaaatg tacctagttt
    58741 aggtacattg atcggttatg caagtaacgg agaagtctta gtgaatcggc aatggatctg
    58801 gttacccgca tcaatcctta ttttagtttt gatgttgagt atcaattatg ttggacaagc
    58861 attcaagcgt tctgcagatg cgcgccaacg gttaggctag tatagaagcg aagggggaaa
    58921 taggaatgaa gaaaacgatt tttggatttg cagcgctgat gtctgctgtc gttcttgcag
    58981 catgcggggg cggaggtaca gatacctctg gaaacaactc tggaggcaac ggcaataaca
    59041 aagaggaatt aacttttcca attgcaacaa aaaataatga tgatgcaatc gaagacggcg
    59101 aattagaagc tgcggtcgca acggacagtc agtttaaagg attgttccag tgggagtttt
    59161 accaagattc atatgatgca gcttttatgg ctccttctca tgaaccgcta tttgcaaacg
    59221 atgacgattt cagaatcaca gacgacggag ctgcaacgct tgatctagat gaagatacga
    59281 aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg
    59341 cggacgatgt gattttccct tacgaaatca ttgggaataa agattacact ggtatccgtt
    59401 atgacgacac tttccgtaac atcgtaggta tggaagaata tcatgatgga agtgccgaca
    59461 caatctccgg aatcaaaaaa atagacgaca aaacagttga aatcgaatat aaagaagtaa
    59521 acccaagcat gcttcaatcg ggtggtggta tctggtcata tgcagcacca aaacatacgt
    59581 tagaaggtgt agcaatcaaa gatatggaat caagcgatca agtacgtaaa aatccagtga
    59641 cttttggccc ttattacatg agtaatatcg ttgcaggaga atcagtagaa tacttgccaa
    59701 acgagcacta ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga
    59761 tggcaagtgc aactgaagca ttgaactcta aattatatga tatgattttc caaatgccaa
    59821 cagatactta cgatacctat aaagaaattg ctggctatac aaacttaggc cgtgaacaaa
    59881 cttcttatac ttatctagga ttcaagttag gtaaatggaa tgcagataca aagagtgtgg
    59941 aatacaatcc agattcaaaa atggctgata aatcattacg tcaagcaatg ggctatgcat
    60001 tagataataa tgcagtaggt gaacgtttct atgcaggttt gagaagcaat gcgacttcat
    60061 tgattcctcc agtattccag tcataccatg atgatgagat gaaaggattc acacaagatc
    60121 tagacaaagc gaaaaaacta ttagatgatg ctggctacaa agatgtggat gacgatggtt
    60181 taagagaaga caaagatgga aataaactaa caatcaactt tgcttcgatg tccggtgggg
    60241 aaacagcgca accattggca gattactata tccaacaatg gaaagaaatt ggtttggatg
    60301 tccaatacac aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg
    60361 atgatcctga aattgatgtt taccaagctg cttggaatac aggaactgat ccaaatccaa
    60421 gtggactatg gggaccaaat gctgcgttca actacactcg ttatgaatca gaagaaaata
    60481 caaaattgat caacgatatc agttcaagca aagcatttga tgaagagtac caaaaagaag
    60541 catatcaaaa atggcaacaa tatgcatttg aagaagcatt tgcgattcca acgttgttcc
    60601 gtaatgaagt attgccagtc aacgaccgtg tcaaagactg ggattggtct tatgatgtac
    60661 caaacccatg ggcaaatgtt tctgttacag cagattcacg ttcataatca ctaaaaatga
    60721 ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt ttctgtttat ttctctaata
    60781 ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa gtccagtttc
    60841 tcggtattaa gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa attttttctt
    60901 catactcgaa actaaacgaa tttttcacaa cttctcgtca atcactagga ttattaaagc
    60961 tttacttacc ttttgaccga accatttcaa atttgacaaa aaaccagtat aataaggaag
    61021 tatcgaaaaa atatccataa aaggaatgtg gaaaatggac aaccagctga caaaagaact
    61081 aaaagaaaac tataacatcg tttttcacga tctaaatttg cttgaacaag cttttaccca
    61141 ttcatcctat gtgaatgagc atcgcaatct gcaattatcg gataatgagc gacttgaatt
    61201 tttaggagat gccgttttag aactgatggt ttctcagtat ttattccgtt tatatcctca
    61261 tttgccagaa ggaaaattga caaaaacacg agctgcgatc gttcgcgaag acagcttgtc
    61321 gaaatttgcc aaagaatgtc actttgatca atatgttttg ttgggcaagg gagaagagaa
    61381 ttcaggagga cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc
    61441 gttatatctt gaccagggat tcgacacgac ccatgcattc atcgaaaaag tgatttttcc
    61501 taaagtcaaa gcaggtgctt tttcacatga gatggatcat aaaacaaaac tacaagaagt
    61561 cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg atcaatgaag aaggtccagc
    61621 tcatgaacga gtattttgga tcgaagttta tgtagatgat caattgattg gcactggaca
    61681 agggaaatcg aaaaaattag cagaacaagc tgcggcggaa aatgcattgg cagcactgta
    61741 aaagtaagtg agggaaagct ttgtatttga aacgaattga aatagccggt tttaaatcat
    61801 ttgcagacaa gacagtcatt gattttgaaa atagcgtcac agcagtggtt ggaccaaatg
    61861 gcagcggaaa aagcaatatt acagaggcta tccgctgggt attaggagaa caatctgcta
    61921 aaagtctgcg aggcggaaaa atgccggata tcatctttgc cggttccgat acccgtaagc
    61981 agctgaatat tgcagaagtc acagtgattt tagacaatag tgatcattat ctgccattgg
    62041 agtacaatga gatcagtgta acccgccgtt atcgacggac tggggaaagc gagtttttca
    62101 tcaataaaca aagctgcaga ttaaaagata tccaagaatt gttcttagat tctggattag
    62161 gaaaagaatc gttctcaatc atttctcaag gaaaagtaga agcgattttc agtagtaaac
    62221 cggaagaccg tcgtgggatc tttgaagaag cagctggtgt cttgaaatac aagcagcgga
    62281 aaaagaaagc agaacagaag ctttttgaaa cagaagacaa cctcagccgc gtccaagaca
    62341 tcatccacga attggaagaa cagttaactc ctttggccgc ccagagtgag gcagcaaagg
    62401 agtttttacg gctaaaagaa acactgactc aaaccgatgt ctctttgatg gttgctgaaa
    62461 tcaagacagc aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag
    62521 aattaggcaa actatctgag tcgatccaag aacaagaatc cattttggcg aagcagcgta
    62581 aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt
    62641 cggaaaaact gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg acaaagcata
    62701 cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa aaggttaaac
    62761 attttgaaaa gcttcaagaa agcttgatga aagcagcagc agaaaaagaa acagagatcc
    62821 aaaaagctga agcaaatctc ataaaaacac agcaagaatt agaaaaatac caaaaatcaa
    62881 cgaaagaatt attggcagaa ttaagagatc aatatgttga tttgatgcaa gaacaagcag
    62941 ctgtaggaaa cgaactgaag tatctagaac gtcaatatat acaagaaaca gcaaaaagca
    63001 agcagacgtt agccaagcaa tcggaagtag aagcatcagt agaccgattg atcttacaaa
    63061 aagaagaatt gacacaaaag caggcgcagc taaaatcatc tttaactgaa acaaaagaga
    63121 agttagaaat gatccaacaa aacgggaaaa agttccaaga agcattagca aaagaacagc
    63181 ctaaaatgta tcaattaatg aatcaagtcc agcagctacg tgctcgtaag aaaagccttc
    63241 aagaaatcca agaaaactat tttggttttt accaaggggt tcgattggtc ttgcagcata
    63301 aacagcagct ttcgggtatt gtcggagcag tggctgaact gatcgatgtt cctgcagatt
    63361 tcacgttagc tatcgaaaca gctttaggtg gggcagccca gcatgtcatc gttgaaaatg
    63421 aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca aagaggagga agagcaacat
    63481 tccttccact gacgacaatc aaaccaagac aattaccggc tcatatattg acacaagcag
    63541 cagctgtaga agggtttata gggattgcca gtgaacaagt cagttatccc gatcaaatcc
    63601 aaactgtggt tcaaaacctg ctaggaacga tccttttagc caaagactta actagtgcca
    63661 atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt ttccttagaa ggtgatgtga
    63721 tgaatgccgg aggatccatg acaggtggag cgaataaacg aggaaaccaa ggcagtttgt
    63781 ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt tgaagaagca gataaacaac
    63841 tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt ttagccgaag
    63901 aacaagaagt tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa
    63961 atcagctaca aaacatcata aatgaacttg aacgttttga aaaagaaaag caaatctcaa
    64021 cttttgagac acgtgaactg cagcagttta tcgaagatta cgaaaagcaa acgaacgaat
    64081 tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat
    64141 cgttaagtca agaaagcgac cagatggaag ccagaagagc acaagtacaa agtcaaaaag
    64201 cacaagaaca agcagatctt gctgtcttga aagagcagtt taatcatctg cagatccagc
    64261 tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg atagaaaagc
    64321 agttggcgac attgacagca gacttttctg atcatgaagt cacagaagaa agtttggaaa
    64381 aacaaattaa cgaattatct gctcagcgag aaacattaaa agccgagttg gcaaaagcca
    64441 aagagcagcg agatagaagg caaaaggaga tcgataagct tgaagcagtc ttggcagagc
    64501 gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa attagaagtc caaaaagacc
    64561 gagctgaaat gattttagat aatcatttga cctatctgca gtcagaatat cagctgacat
    64621 ttgagaaagc ttcccaagac tatcaagaaa caaccgatat cgaagacagt agaacaaagg
    64681 tttcatcctt aaaagaacag attgaaaaac tgggaccagt aaacttaaat tcaatcgaac
    64741 aatacgaaca agtcagtgaa agacatacat tcttagcaac acagcgagat gatctgttag
    64801 cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga tgatgaagtg cggacaagat
    64861 tcaaagaagt atttgaagct atccgtcaag aattcaaaat tgtctttcca aatatgtttg
    64921 gcggcggtaa agctgaatta gtactgacgg aaccaactga tttgctgaaa acaggtattg
    64981 agattgaagt acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag
    65041 aacgagcact tacagccatc gcgttgctct tttccattat ccgtgtctgt cctgttccgt
    65101 tttgtatctt agatgaggta gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc
    65161 gttacttgag cgatttccaa gatgacacac aatttatcgt agtcacacac cgcaaaggaa
    65221 cgatggtcgc agcagatgta ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa
    65281 tcgtttctgt acggatggaa gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt
    65341 aggaacgaaa atgatcaaat tagtagcaat cgatttggac ggtacattat taaatgaaca
    65401 aaagctgatt tcagatgaga ataaacaagc attagcacaa gcaaaacagc aaggggtcaa
    65461 gatcgttcta tgtacaggac gccccttagc tgcgatggcg cattatctgc aagaattagg
    65521 actggtagat gaaggtgact acagcatcac tttcaacggc ggtttggtac aaaaaaatga
    65581 tactggtgaa atcatggaaa agaaagtgat ggaagtatca gatatccaac gcttatacaa
    65641 actggctcag caattggatc tgccactaga tgttttgtct gacaatgtgg ttttacagct
    65701 gcctagtgcc ccaaggaagc aatccttgta caatgtgttg aataagctgc tgcaattcca
    65761 accagcggat ataagcgata ttacagatga atttgtgctt aataaggcag taatcgctta
    65821 tgaccaagaa gaattagatc ctaaaatcaa agaaatacca gcagaattcc atgaaatgta
    65881 tgagatcatc aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc
    65941 ttatggtatt tctttattgg caaaagatct tggtttgaaa caagaagaaa tcatggcgat
    66001 cggtgatgag gaaaatgatt taccgatgat ccaatatgct ggactaggcg tagcgatgga
    66061 gaatgctgtg cctttcgtca aagaagcagc agatttcgtg acagccagca atgaagaaaa
    66121 tggcgtagcc aaagtgatcc aaaaatttgt attagagtag ggggagtcaa atgggatttt
    66181 ttgataaaat caagcgtgct tttactggcg aagagaaaac agaagaagtt gaagtacagg
    66241 agaagtacga caagggttta gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt
    66301 tgtttgccaa ttttcgaaca gtcgatgaag acttttttga agaattagaa gaaacactta
    66361 tcggtgcaga tgtaggtttt gaaacagcga tacgaattac cgaagcatta cgtcaagaag
    66421 tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg
    66481 tggatctcta tgaagcggaa ggaatcaatg aagtaaatga actaaatatc caagaagacg
    66541 gactaacagt tattttgttt gtcggtgtta atggtgtagg gaaaacaaca agtattggta
    66601 aacttgccca tcagttcaaa aacgaaggta aaaaagtatt gatggcagca gcagatacct
    66661 tccgcgctgg agcaatcgac cagttagttg tctggggcga acgcgctggt gttgaagttg
    66721 ttcgtggcaa tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa
    66781 agacagagca ggcagatgtt ttactagttg atactgcagg ccgactgcaa aataaagtga
    66841 acttgatgaa cgaattagaa aaaataaaac gagtaatcca gcgggaatta ccagaagcac
    66901 cccaagaaac acttcttgta ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa
    66961 aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta gatggtacag
    67021 caaagggcgg tatcgtactt gctatccgta atgaattgca tttaccagtg aaattggtcg
    67081 gtcttggtga aggaatcgat gacttggagc cgtttgatcc aaatgatttt gtcgttggat
    67141 tgttcaaagg attactaaaa gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa
    67201 agttgcagct ccttgtttga atgattgttt ttaacatagc tgtgtttttg atgccttcat
    67261 ttttatactt atttgtaagc ggttctcaaa caatttcgtt ctttttctta gactttttga
    67321 ttataattag attcatatga attggaggtc acttaatgat gaattttaag caattattat
    67381 tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt tggtatagaa cgggaaggac
    67441 agcgggttga tcttgcagga aatctagcaa aaaccgatca tccagcaatc tttggcgatc
    67501 gatcctatca cccctatatc caaacagatt ttagcgaaac acaaacagag atgatcaccc
    67561 ctgttaccga ttctattccc gaattatttc agtatctcgc tgctgtttat gatgtgactg
    67621 ctcgttctat accgaaagaa gagatgatct ggccattaag tatgccacct gccttaccgg
    67681 aaaaagacga agagatcatt attgcaaaat taaaaaattt cgaagatgtc ttgtatcgac
    67741 gttatttagc aaaagaatac gggaagcgaa aacagatggt aagcggtatt catttcaatt
    67801 ttgaatttgg cgatgagttg ctaagaacat tgttcagcca tcaggaagaa tttcaagatt
    67861 tttccgaatt caaaacagaa ctttatttga aaacagcgag aaactttatg cgctatcgct
    67921 ggatgatcac ttatttattc ggtgcttcac caatgagtga gaagaactac tttttagatg
    67981 aatcacatcc gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc tatacgaatc
    68041 atccaaatgt gaaagtttcc tatgcttcta tgaaacagta tttagcagat atcgagcgaa
    68101 tgatcgaaga agggaaactt tcggaggaga aagaatttta tacaccgctt cgtttccgag
    68161 gcggaaagaa agtcgcagat ttagcaacaa caggtgttcg ttatattgaa ttgcggaata
    68221 tcgatttgaa tccttatgca agattgggta tcaatccaga gcaagttcgg ttcttacaat
    68281 tattcctgat gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa tgggtagcag
    68341 aaggtacgac tcgaaataac aaagtagcac tagaacaacc atcagatcaa acagaatttc
    68401 atcaagaagg tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa ttggattggt
    68461 tggattctct ttatttagta gaggaagcat tgactcaaat ggatcatcct gaacaaacgt
    68521 tagcagcgaa actctatcaa gaagcacagc tatcgagtca gcaagaagtt gctgtggcat
    68581 tgggacatca atattataaa gaaagccatg aacgtcccta tcagttggcc ggttttcgcg
    68641 aaatggaact ttctactcag atatttatgt tcgatgccat ccaaaaaggt gtccaagtca
    68701 aagtattaga tgagtcggat caatttttgc gtctgcaatt ccaagaccat gtagaatatg
    68761 tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt cccacttatc atggaaaaca
    68821 aaacagtaac gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga ggcgcagaat
    68881 tttcatccat ggaagaagcg gtaaaagctt atccgagatt tgccgagcag gcatttgtga
    68941 tcaaacctaa atcaaccaat tacgggttag gtatcacgat tttcaaggaa ggagctagtc
    69001 tggaagatta tcaagcaggt ttagccattg cttttcgtga agacagttcg gttttagtag
    69061 aagaatttat gccgggaacg gaatatcgtt tctttgtgat cgacggagaa gtccaagcca
    69121 tcatgttgcg agtccctgct aatgttattg gtgacagtat ccgaacagtg aaagaacttg
    69181 ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct ttagaattga
    69241 tccaattagg tgaattagaa caattgatgt tgaaagaaca agggttgacg atcgaatcag
    69301 ttccccaagc caatcaaatt gtttatctaa gggagaactc aaatatcagt acaggtggtg
    69361 attcgatcga tatgactgat gaattttctg aagcttacaa aaaaatcgcc gtttctgcgg
    69421 tagaagcact aggagctaag attagtggta tcgatttgat tataccagat aaagaaatcg
    69481 atcctacaac tgataaaaaa gcatatggga tcatcgaagc aaatttcaat ccagccatgc
    69541 atatgcatgt ttatcctttt gcaggaaaag gcagaagatt gacgatgaat gtattgaaat
    69601 tgttatatcc tgaagtattt taaaagaaaa agcttttgac agattgtcct atttgatgtc
    69661 aatctgatca aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt tttaaaataa
    69721 acgatcccag aagctttttt tcttttttgg tttttctttt tcagctggcg aagagttttc
    69781 ttctgggtat tttttttcga tatcaatcac aggttcattt actgcttctt ttgctgttaa
    69841 gagcaatccg aattcctcag gatttgcagt gacgtaatcg ttgactacag tgaaacggct
    69901 attgtttttc gttgctagct ggatatattg gttttggaca gaaaggggta gagccccatt
    69961 caagagtatg gaagcatctg gatgtttttt gagttctttt aaaaagtttt ctttgttttt
    70021 tggttggatc atttcagcaa cagtcatact aagatagcag cgttccctga atgtccctaa
    70081 atatttatgc tgttcttcag ggttcactaa aggtgtacca taaagtccct tatccaaatg
    70141 gctttttaca tcatcttgtg ccataaaaaa taccctcctt cttctctttg tctttcttac
    70201 gattatagca tattcccaat aatacctaaa acatatgaaa aagcggttgc ttttgttcaa
    70261 cgattcactt aaaataagaa tgaaaatcat tctcaattag aaaggtggat tggatgcgct
    70321 acggacgact agtagcatta ttagttattt taagtagtat ttcattgttc atcggagtat
    70381 cgaatgtaca attgtcagaa ctgctccagt tagatgggca aagcttacaa atcttgctag
    70441 tgactcgttt gccacgaacg atttgtttga tccttgtggg agcgaccagt agtatttgtg
    70501 ggttgatcat gcaacatttg acccaaaata aatttgtttc accaacgact gcgggcacga
    70561 tggatagtgc gcgtttaggt attttagtgg ctatgatttt cctacctggt gcatccttga
    70621 ttgtccgatc cttgacagca ttttgctttg cttttgcagg aacattgctt tttttatccc
    70681 ttacgcgatt gttcctccag aaaaatcaat tgatgcttcc gttgattgga gtcatgttag
    70741 ggaatatcat tggttcagtt gctacttttt ttgcttatca attccagttg gtacaaaata
    70801 tgacttcatg gcttcaaggg aattttgcga ctgtgatacg aggaaattat gagttattgt
    70861 atgctgttgt tccattgttg ctcttggttt ggctatttgc ttaccgattt acgatcattg
    70921 gtctgggcga atcgtttgca gtaaatctgg gaatagatta ccagaagatg cagtttttag
    70981 gcattggctt agtatcattg gctagtgcag tcatgttgat catggtagga agcattcctt
    71041 ttatcggagt gatcattccg aatcttgtct ctagaatata tggagatcaa gtgcacaaaa
    71101 caattggcgt aactgcttta gcaggtagtt tgttcttagt attttgcgat cttgtcgctc
    71161 gcgtgttgat ttttccttat gaaataccag tcagtgtgat cgttggaatc atcggtgggg
    71221 ctatcttcct ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa gccatctatt
    71281 tttattggta tgcttgttct tgttttattt gttacaggaa tgtacttgac gatccataca
    71341 tatggaaact ggcattttgc atgtgcattg aggagtaaga aaatttttgc atttatcctt
    71401 gtctcgctag ctgtcagcag tgccacaatc agctttcaga caattacgca taaccagttt
    71461 ttgactcctt cagtattggg acttgagtcg ttatatgtcc ttgtccaaac ggtccttttc
    71521 ttctttatcg gaggagtgac gatgcttcag cagacaggta cagttttttt cttattgaac
    71581 gttgggctga tggtcgggct gagtctattg ttatttttct ttcttttgaa aaaaatgaga
    71641 gcgaatcttt ttttattact gatgtcaggg atgattttag gtacgctttt tggtagtgtc
    71701 agtacctttt tacaagtcat catggatccg aatgaatatg atttgcttca aggcagatta
    71761 tttgcaagct ttggcaatat ccagagtcag tatcttttgc ctgcttcttt gatcattatc
    71821 ggctgctggt acctgttatt tcgatatcga catgtgctag acgttttcca tctcggaaat
    71881 gaacaagcag taaacctcgg gatagacaca gatcgttttg aatttttgat tttatgttgt
    71941 gtcagtcttt tgacagggac atcaacggct cttgtgggtc cagttacttt tttagggttt
    72001 attgtcgcaa atatcagtta tcgtgtcttt tctacttatc gtcatggtca gctatttatc
    72061 ggaagctttc tggtagctat gctgtttctg acaggaggac agctcgcagt agagcagatt
    72121 ttcggctgga atacaacagt gagtgtcgtc gttgaatttg tcggaggaat ttattttgtc
    72181 acaaaattga ttcgcgaaag gaagagtatc ctatgattga tttacaaaag gtcaataaac
    72241 tgtaccatgg aaaatcagtg gttaaagatg tacagttaac gattcctgaa aagaaattga
    72301 ctgcttttat cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg atcagtcgtt
    72361 tgataccaaa agatacaggt gaaatctatt tagaccataa cgaagtaaag gcttggaaat
    72421 ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata aatgtccaaa
    72481 tcaccgtgaa agaattagta gaatttggac gatttcctta tacgaaagga aaattgacga
    72541 caaaagacga ggaaatcgtt caggaatcac tggaacacct tgggttagaa gaaatagcgg
    72601 atcaatcgat tgacactctt tcaggcggtc aactccaacg tgcgtatatt gcaatggtgc
    72661 tagcgcaaga cacggattat attttactgg atgaaccttt gaacaatcta gatatgaatt
    72721 ttgcggttca aatcatgcag attttaaaaa atttagtaca agaaatcggg aaaacaattg
    72781 ttattgttct tcatgatatc aactttgctg caagttatgc agacgaaatt gttgcaatga
    72841 aagatggaca gttatttacg caaggaacga cccaagaaat gataaaaaaa gatgtattgg
    72901 atgaattata cgaaatggac atacggattt gtgagattga aggccgcaga ttctgtcttt
    72961 atttctaaaa ctaacattag gagagaagag atgaaaaaat tagtagtagg tatcttgatt
    73021 ctagcatcat ttggtttagc agcatgtgga aatagcaaca acaccagtca ggcagacaca
    73081 aaaaaaagca gcacacaaac tgaaactaca ttgacgataa cagatagtaa tggagaccaa
    73141 atcgaggtgc caaataatcc agaaaaagtc gtcgtattcg acaatggttc tttagatacg
    73201 atggatgctc ttggtgttgg agataaggta gttggagcag ctacaagcag ccttcctgag
    73261 tatctttcat cctataaaaa agtagaatca gcaggtggaa taaaagaacc agacttagaa
    73321 aaaattaatc agcttcagcc agatctaatc atcatttctg gccgacaaag agattttcaa
    73381 aaagatctaa gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa aaatccatgg
    73441 gaatctttcc agcaaaatgt cactgcttta ggggagatct ttggtaagca agaagaagct
    73501 aaaactcaat tagaggaatt atctagtgca atcgatcaga cgaaaaagaa agcagaagct
    73561 actgacaaaa aagcactagt cacacttgtg aatgaagggc aattatcagc ctacggatct
    73621 ggttctagat tcggtttcat ccatgattta tttggctttg aacaagcaga tgatcaaatc
    73681 gaagcatcca ctcacggtca aagtgtttct tatgaatatg tgctggaaaa aaatccggat
    73741 atcttatttg tagtagacag aacaaaagct attggtggag acgacagcaa agatgatatt
    73801 tctgccaatg aactggtagc acaaacgaat gctggtaaaa atcagcagat catctccctt
    73861 gaaccagatg tctggtattt aagcgggggc ggtttagaat caatgaaact gatgatcgaa
    73921 gatgtgaatc aagcatttaa atagcataaa taacagagga ttaaactctc atactatgta
    73981 acaataatgt ttcttacatt cttaaatagc aagagcctat cctcaaagaa gttatcaaaa
    74041 accatccgta aaatcgtatt gatgatttta cggatggttt ttatttattt gagaaatctt
    74101 ttttcaacgt gaattcatta tctacatcat acgggttgat tctagaatgg ggagtggcaa
    74161 aaaatagtgt gatccatttt ttcagcatca agatcgcctc ctttgatttg aatcaaaggt
    74221 acaaagcgaa acttagataa aacttaaatt tagatatttt tattttgtac cgatctcttc
    74281 tttaaacgat gttccatctg ctgcttctcg gcaataagtc cactagacta gaaaatatga
    74341 gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc
    74401 ttctaaacga tgttccatct gctgcttctc ggcaataagc ccactagact agaaaatatg
    74461 agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt gtcacaatct
    74521 cttctaaaca atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa
    74581 cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat
    74641 aaaaatcggc gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg
    74701 ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt
    74761 ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa
    74821 tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct
    74881 tcatagtgat tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt
    74941 taataatccg tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt
    75001 ttatagtatt ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt
    75061 tgggtctttt tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat
    75121 aagagaaatc ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca
    75181 gacaaattag ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat
    75241 gaaattagaa gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga
    75301 tcaactttta ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg
    75361 ccaattggat acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag
    75421 tctttattct gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat
    75481 actttacatg gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt
    75541 aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga
    75601 taaaatggga aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga
    75661 atcagagctg atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct
    75721 agccggagat acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa
    75781 taaaggatta tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga
    75841 acagatgatg gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa
    75901 tgagcagacc gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa
    75961 agattacgag gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg
    76021 tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga
    76081 ccagctggaa gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc
    76141 atttatcatg tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg
    76201 atcagaatca ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg
    76261 ccatacgcat cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc
    76321 ttttggttat tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct
    76381 agacagctat gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca
    76441 ggcctatcaa acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta
    76501 ttgagacagc attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag
    76561 agcagtagat ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg
    76621 tttatttgat cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt
    76681 ttgaacgata atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat
    76741 tgattgaagt ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta
    76801 ttttaaaggc tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac
    76861 tagaaatcaa atcgggctga taatcattta taactaatcg atacatagga ccaaatatag
    76921 gtctatgttc ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa
    76981 tcgactgata tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt
    77041 attaacagca gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac
    77101 tgaagtatac ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga
    77161 gggtgtaact cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc
    77221 agatcctcca agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa
    77281 atttgggact caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat
    77341 gaacgatggg tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc
    77401 tgggtggaaa ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact
    77461 agttggagca cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata
    77521 tacgccgcaa acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc
    77581 aattaatgct aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga
    77641 aaatgaaaat caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca
    77701 acaatattcc actaaattag tttggacatt agaagataca cctaacaact agtttactga
    77761 aaagaactgt taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat
    77821 tgtcaaaggc taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc
    77881 aggaggaaaa gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt
    77941 gactaatatc tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa
    78001 tagaagagac gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca
    78061 gacacaagaa agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt
    78121 agaagctgag ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct
    78181 gcctgacgat cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag
    78241 cacgtccata ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag
    78301 aggagctatt tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta
    78361 tttatactta ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg
    78421 cgatcctaga atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg
    78481 ctcatattct actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga
    78541 tacgtataaa aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag
    78601 agggcatgga catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga
    78661 gcatactggc gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat
    78721 aattaaatgg gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcactc
    78781 ttctcggatt atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt
    78841 tacttcttca acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc
    78901 gaatgtcaca tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga
    78961 agctggagga aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct
    79021 tgaatataag aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca
    79081 tatcttgatc aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt
    79141 cacgctaaat ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac
    79201 aactttgcag gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa
    79261 cgggaatttg attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat
    79321 agatatccat gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac
    79381 aatcactggg aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt
    79441 ttttgactcg gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta
    79501 tttttattac agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg
    79561 aaaacataaa atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa
    79621 aattatagat gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct
    79681 tacaaatgga gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac
    79741 ggttataggg acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt
    79801 gtcctctctt ttagattcta cagctcatcg tggaatcaga atagatattc ctgtagaaaa
    79861 ccaaagaatc ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa
    79921 ttaacaggaa gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat
    79981 aggctcttgt gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa
    80041 ggagcgattt tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg
    80101 gcacttttta acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata
    80161 acttcagaga atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat
    80221 caggaagtag aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat
    80281 atttctgtga atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag
    80341 attgaaaaag acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata
    80401 gtgacgctaa aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag
    80461 cgatttgatg gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac
    80521 caaacagaaa ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct
    80581 ttgttgatgc aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa
    80641 ccagatcaaa tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc
    80701 tatatcaatc aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta
    80761 tatcaagagg aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact
    80821 gctttaaatg gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga
    80881 aatgaaaatg aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc
    80941 aatcaatggg tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac
    81001 acgacggatg ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt
    81061 ttcttattgt ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa
    81121 gaaaagtaag gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct
    81181 ttttataaca atataaaata taccatttta ttaataaaaa tgcatactag taaatgatat
    81241 aattctaata acaggtttga tcgcgagaaa ggccaaataa aacgctctcc aaaatgttaa
    81301 aaaaaagaca ggacgatgta tattatacaa acaagaatcg ctgacaacag tgactcggat
    81361 ccaggaaatc agttttatcc ccagctagtt ggtgaatgca tggaaagtaa tatacgtaga
    81421 aagagctgat gactaatgaa ttatgagttt tttttaagca aagaagatca cagaagttat
    81481 tcattactta aatatttaga agcaagctca aatttatcag agtcaatatc gaatgttcaa
    81541 gaagagttgt cgctatcgac atttctcttg aaaaaaacga tcgataagct gaaacatgat
    81601 ttacaaaagt tcgatctaga gaaaaatttg cgagtaaccg tcagtgaatt ggatatatgt
    81661 ctggaaatta atggaaaatg ttctagcaaa gctctgttat ctcgttatat aacggattct
    81721 ttatctatga aaatggtatt ggctttcttt caggaaaaat atatctcagt agaaaatttt
    81781 gctaaacagc atcacgtgag ttattctgtg gcttataaag tattacaaag actaaaaaga
    81841 aatctaaaaa aatatcaaat tttttttgaa aagagaaagt tga
//
LOCUS       DAPBAB010000007        78114 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPBAB010000007 DAPBAB010000000
VERSION     DAPBAB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 78114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.007"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..106)
                     /locus_tag="RD444_000631"
     rRNA            complement(<1..106)
                     /locus_tag="RD444_000631"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            847..1536
                     /locus_tag="RD444_000632"
     CDS             847..1536
                     /locus_tag="RD444_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7548024.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1523..2731
                     /locus_tag="RD444_000633"
     CDS             1523..2731
                     /locus_tag="RD444_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7548025.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3025..4296
                     /gene="serS"
                     /locus_tag="RD444_000634"
     CDS             3025..4296
                     /gene="serS"
                     /locus_tag="RD444_000634"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDT7548026.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="RD444_000635"
     CDS             complement(4845..6329)
                     /gene="guaB"
                     /locus_tag="RD444_000635"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDT7548027.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6834..7529)
                     /locus_tag="RD444_000636"
     CDS             complement(6834..7529)
                     /locus_tag="RD444_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDT7548028.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="RD444_000637"
     CDS             complement(7619..8719)
                     /gene="ychF"
                     /locus_tag="RD444_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDT7548029.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8728..8919)
                     /locus_tag="RD444_000638"
     CDS             complement(8728..8919)
                     /locus_tag="RD444_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDT7548030.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8940..9830)
                     /locus_tag="RD444_000639"
     CDS             complement(8940..9830)
                     /locus_tag="RD444_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDT7548031.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9817..10584)
                     /locus_tag="RD444_000640"
     CDS             complement(9817..10584)
                     /locus_tag="RD444_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7548032.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="RD444_000641"
     CDS             complement(10596..11309)
                     /gene="rsmG"
                     /locus_tag="RD444_000641"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDT7548033.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="RD444_000642"
     CDS             complement(11541..13442)
                     /gene="mnmG"
                     /locus_tag="RD444_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDT7548034.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="RD444_000643"
     CDS             complement(13459..14856)
                     /gene="mnmE"
                     /locus_tag="RD444_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDT7548035.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15155..15538
                     /locus_tag="RD444_000644"
     CDS             15155..15538
                     /locus_tag="RD444_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548036.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15706..16464)
                     /locus_tag="RD444_000645"
     CDS             complement(15706..16464)
                     /locus_tag="RD444_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDT7548037.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16482..17300)
                     /locus_tag="RD444_000646"
     CDS             complement(16482..17300)
                     /locus_tag="RD444_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDT7548038.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="RD444_000647"
     CDS             complement(17297..17653)
                     /gene="rnpA"
                     /locus_tag="RD444_000647"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDT7548039.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="RD444_000648"
     CDS             complement(17771..17905)
                     /gene="rpmH"
                     /locus_tag="RD444_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDT7548040.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18428..19762
                     /gene="dnaA"
                     /locus_tag="RD444_000649"
     CDS             18428..19762
                     /gene="dnaA"
                     /locus_tag="RD444_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDT7548041.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19960..21090
                     /gene="dnaN"
                     /locus_tag="RD444_000650"
     CDS             19960..21090
                     /gene="dnaN"
                     /locus_tag="RD444_000650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDT7548042.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21319..21561
                     /gene="yaaA"
                     /locus_tag="RD444_000651"
     CDS             21319..21561
                     /gene="yaaA"
                     /locus_tag="RD444_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDT7548043.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21548..22672
                     /gene="recF"
                     /locus_tag="RD444_000652"
     CDS             21548..22672
                     /gene="recF"
                     /locus_tag="RD444_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDT7548044.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22669..24615
                     /gene="gyrB"
                     /locus_tag="RD444_000653"
     CDS             22669..24615
                     /gene="gyrB"
                     /locus_tag="RD444_000653"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDT7548045.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24635..27106
                     /gene="gyrA"
                     /locus_tag="RD444_000654"
     CDS             24635..27106
                     /gene="gyrA"
                     /locus_tag="RD444_000654"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDT7548046.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27342..27641
                     /locus_tag="RD444_000655"
     CDS             27342..27641
                     /locus_tag="RD444_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDT7548047.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27691..28203
                     /gene="ssb"
                     /locus_tag="RD444_000656"
     CDS             27691..28203
                     /gene="ssb"
                     /locus_tag="RD444_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7548048.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28229..28465
                     /locus_tag="RD444_000657"
     CDS             28229..28465
                     /locus_tag="RD444_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDT7548049.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28608..30581
                     /locus_tag="RD444_000658"
     CDS             28608..30581
                     /locus_tag="RD444_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDT7548050.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30587..31039
                     /gene="rplI"
                     /locus_tag="RD444_000659"
     CDS             30587..31039
                     /gene="rplI"
                     /locus_tag="RD444_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDT7548051.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31313..32680
                     /gene="dnaB"
                     /locus_tag="RD444_000660"
     CDS             31313..32680
                     /gene="dnaB"
                     /locus_tag="RD444_000660"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDT7548052.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33460..34752
                     /locus_tag="RD444_000661"
     CDS             33460..34752
                     /locus_tag="RD444_000661"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDT7548053.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35488..36351
                     /locus_tag="RD444_000662"
     CDS             35488..36351
                     /locus_tag="RD444_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7548054.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36480..38102)
                     /locus_tag="RD444_000663"
     CDS             complement(36480..38102)
                     /locus_tag="RD444_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7548055.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38351..38872
                     /locus_tag="RD444_000664"
     CDS             38351..38872
                     /locus_tag="RD444_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDT7548056.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(39058..40788)
                     /gene="spxB"
                     /locus_tag="RD444_000665"
     CDS             complement(39058..40788)
                     /gene="spxB"
                     /locus_tag="RD444_000665"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDT7548057.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40953..41771
                     /locus_tag="RD444_000666"
     CDS             40953..41771
                     /locus_tag="RD444_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDT7548058.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41874..42071)
                     /locus_tag="RD444_000667"
     CDS             complement(41874..42071)
                     /locus_tag="RD444_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548059.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42240..42818
                     /locus_tag="RD444_000668"
     CDS             42240..42818
                     /locus_tag="RD444_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDT7548060.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42913..43050
                     /locus_tag="RD444_000669"
     CDS             42913..43050
                     /locus_tag="RD444_000669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548061.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43863..44366)
                     /locus_tag="RD444_000670"
     CDS             complement(43863..44366)
                     /locus_tag="RD444_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7548062.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44725..45750
                     /locus_tag="RD444_000671"
     CDS             44725..45750
                     /locus_tag="RD444_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7548063.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            46097..46975
                     /locus_tag="RD444_000672"
     CDS             46097..46975
                     /locus_tag="RD444_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDT7548064.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47451..47567
                     /locus_tag="RD444_000673"
     CDS             47451..47567
                     /locus_tag="RD444_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDT7548065.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48495..48833
                     /locus_tag="RD444_000674"
     CDS             48495..48833
                     /locus_tag="RD444_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548066.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48927..49157
                     /locus_tag="RD444_000675"
     CDS             48927..49157
                     /locus_tag="RD444_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548067.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49557..49928
                     /locus_tag="RD444_000676"
     CDS             49557..49928
                     /locus_tag="RD444_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDT7548068.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(50202..50894)
                     /locus_tag="RD444_000677"
     CDS             complement(50202..50894)
                     /locus_tag="RD444_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7548069.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50897..51682)
                     /locus_tag="RD444_000678"
     CDS             complement(50897..51682)
                     /locus_tag="RD444_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7548070.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51919..53319
                     /gene="lacG"
                     /locus_tag="RD444_000679"
     CDS             51919..53319
                     /gene="lacG"
                     /locus_tag="RD444_000679"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDT7548071.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53344..53661
                     /locus_tag="RD444_000680"
     CDS             53344..53661
                     /locus_tag="RD444_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7548072.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53674..55347
                     /locus_tag="RD444_000681"
     CDS             53674..55347
                     /locus_tag="RD444_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDT7548073.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55361..56992
                     /locus_tag="RD444_000682"
     CDS             55361..56992
                     /locus_tag="RD444_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDT7548074.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57864..59054
                     /locus_tag="RD444_000683"
     CDS             57864..59054
                     /locus_tag="RD444_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDT7548075.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            59188..61377
                     /locus_tag="RD444_000684"
     CDS             59188..61377
                     /locus_tag="RD444_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7548076.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61374..63167
                     /locus_tag="RD444_000685"
     CDS             61374..63167
                     /locus_tag="RD444_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDT7548077.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            63367..63681
                     /locus_tag="RD444_000686"
     CDS             63367..63681
                     /locus_tag="RD444_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7548078.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            63702..64079
                     /locus_tag="RD444_000687"
     CDS             63702..64079
                     /locus_tag="RD444_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7548079.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            64089..64862
                     /locus_tag="RD444_000688"
     CDS             64089..64862
                     /locus_tag="RD444_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548080.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            64884..66287
                     /locus_tag="RD444_000689"
     CDS             64884..66287
                     /locus_tag="RD444_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDT7548081.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            66469..67653
                     /locus_tag="RD444_000690"
     CDS             66469..67653
                     /locus_tag="RD444_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7548082.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            67650..67940
                     /locus_tag="RD444_000691"
     CDS             67650..67940
                     /locus_tag="RD444_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548083.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            67937..68158
                     /locus_tag="RD444_000692"
     CDS             67937..68158
                     /locus_tag="RD444_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548084.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            68200..68979
                     /locus_tag="RD444_000693"
     CDS             68200..68979
                     /locus_tag="RD444_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDT7548085.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            69046..69540
                     /locus_tag="RD444_000694"
     CDS             69046..69540
                     /locus_tag="RD444_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDT7548086.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            69596..70234
                     /locus_tag="RD444_000695"
     CDS             69596..70234
                     /locus_tag="RD444_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548087.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            70306..70701
                     /locus_tag="RD444_000696"
     CDS             70306..70701
                     /locus_tag="RD444_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7548088.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            70685..73138
                     /locus_tag="RD444_000697"
     CDS             70685..73138
                     /locus_tag="RD444_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7548089.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            73135..75162
                     /locus_tag="RD444_000698"
     CDS             73135..75162
                     /locus_tag="RD444_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548090.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            75159..76181
                     /locus_tag="RD444_000699"
     CDS             75159..76181
                     /locus_tag="RD444_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDT7548091.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            76198..77127
                     /locus_tag="RD444_000700"
     CDS             76198..77127
                     /locus_tag="RD444_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7548092.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            77372..77488
                     /locus_tag="RD444_000701"
     CDS             77372..77488
                     /locus_tag="RD444_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDT7548093.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            77504..>78114
                     /locus_tag="RD444_000702"
     CDS             77504..>78114
                     /locus_tag="RD444_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7548094.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQE"
BASE COUNT        24782 a        13951 c        15437 g        23944 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcaggag tgtttcccga ttcgtttttg gctgccgtag caacttttat
      301 atcatatcag agagacaagt aattgtcaac tcttttttaa aagttttttc gatttcgttt
      361 ttcgaaatct tccagcaact catttatcat aacttatcgt ttgttatttg tcaagaactt
      421 ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg ttctttttta acgactcagt
      481 tatcttatca tgtcatttcc aacatgtcaa caacttattt tttattattt aataagttat
      541 tggtgttctt ttgaacaaat aataatatat caacatcaca tgtgaaagtc aataagaaaa
      601 tgcttattta tcaacatttc tagcttacat gatcattggt tagttctggt cctttttcgt
      661 tcgtctttat aactccgaga gacgtaaaat ccttttgttt tcagtttaat gttcatgttc
      721 ttgtcttatt cacaccaact agatgtctca ccttctaaaa ttttccttaa caaagtgctg
      781 cttttatcta tttctgcttt tttttgctag aataatagta acaatatgac tctggaggat
      841 ttatttatga aaattttgat tgctgacgat gataaagaaa tcgttgaatt attaagtatc
      901 tatgttcaca atgaggggta tgaagcagta aaggcttatg atgggaaaga agcattatct
      961 aaaatcagaa caatccctga tattgaatta cttattttag atatcatgat gcctgagatg
     1021 gatgggatgc aagtagtgaa agagctacga aaagaatccc aaataccaat cattatgctg
     1081 acagcaaaaa ctactgatat ggataagatc aaaggactgg tcgctggtgc agatgattat
     1141 gtgaccaagc catttaatcc gttagaagta atggctagag taaagtcttt attacggcgt
     1201 acaaaaatgc agattgcccc agatcagcct gatgtattag aagtaaatgc attaatcatc
     1261 aataaagact ctcatgaagt taaaacaatc gatgggaaag aaatccagtt aacagctttg
     1321 gaattcggta tcctctactt gcttgctagt catcctaatc gagtatttag cgcggatgaa
     1381 atttttgagc gtgtttggaa acaagaaagt attgtctctg ctaaaactgt catggttcac
     1441 gttagtcatt taagagataa gattgaagaa gcaactggtg gtgaaaaagt cattcaaacc
     1501 gtttggggag tgggctataa aattgacgca agatagtaaa cgagcaactg aaaaacgccg
     1561 tcggatcaat ttgacttcaa aagaaatcag cgaattgtta gcggaaggaa tcatcacgat
     1621 tatccttttg cttctattaa atgtttctat tctggttgtt atcagttcgg tgatcaacag
     1681 ttcaccttct ttgacgaatg cgatttggga ttcaaagaat atttttgcag aacgattgaa
     1741 cactgatttg ttctggaacg gccggaattt tattattccc tttttctttc tattagatat
     1801 tggtgttttg tattggcgct tgattcgaag atatcgccaa atgcaattgc ggcatatcat
     1861 cagcgaactt cactacatcg caaacggaaa ttatgatcat cgtatccctt ttgaactgag
     1921 tggtgactta agccgagtgg taacaagtat caatggattg gtggatagta ccgtagcagc
     1981 gattgaagac gaacgtaaaa tcgaaaaatc aaaagatgaa ttgatcacga atgtcagtca
     2041 tgatatccgt accccactca cgtccatcat tggataccta ggactgatag aagatggcca
     2101 gtatcactca gaagaagatc ttttaaagta tacgcatact gcttatatca aagcaaagca
     2161 gatgaaatct ttagtggacg acttatttga atacactaag gtacgccagc ctgctgtccc
     2221 ggtgaatttt tctgcctttg acatgatcca gttgatcgaa cagttagcag ctgattttga
     2281 gttggaagct tctaaaaaaa atatccaaat acttgttcaa tcaaaagtag actcccttat
     2341 catggatggc gataccgaaa aacttgtacg tgtcttcaat aatcttttga cgaatgcgtt
     2401 gaaatacgga aaaggagcaa cgaagatcgt aatcgaagta gaacgtatcg gttcagaagt
     2461 tgttgcaaca gtcaaaaata acggagcaat gatcccacaa caggcaatcg ataatctatt
     2521 cgaccgtttt tatcgtgtag aagaatctcg ttcacaagca actggaggaa ctggacttgg
     2581 tttagcaatc gctcaaagca ttgtggcttt gcacggcgga tacatttatg caaaatcaga
     2641 taagcaatgg acttctttca tcatgcacct tccaatcaag aaaaatgaaa aattacctat
     2701 tgatacacag gaagttattg acgaaagctg aaatattcgc tattcttatt acaattatta
     2761 gaataaatga aaaacacaga acccagtaca ctttattctt tttcagagag ctgatggcag
     2821 gtgtaaatca gcaaaggata atttggaagt tccagttcgt ttacgtgccc tttcgaaata
     2881 agaaaggtcg gttcaacagc cgttatgtgt tcaacaagtg cagctgacgc tgaacttggg
     2941 tggtaccgcg aatttcagac tgattcgtcc caagaataat gggatggatc agtctttttt
     3001 tattttcaaa aaggaggaaa tcatatgtta gatgtaaaaa tgatgcgaca aaatattgaa
     3061 gaagtacaag aaaaattgat gactcggggc gtaaaaaaag aaacattagc tgaattcatg
     3121 gaactagatg aaaatcgtcg taagctacta gtcaaaagtg aagaattaaa aaaatatcga
     3181 aatgatgttt ctgctgagat cgcccaattg aagcgtgcaa aagaagatgc ttctgctaaa
     3241 atcgctgaaa tgaaagaagt cggtggcaac atcaaagcat tggatactga aattgctgaa
     3301 gtggatgaaa aattgaaaca aattgcaact actttgccaa accttcccca tgattctgtg
     3361 ccagttggaa cagatgaaga ggacaatgta gaaatccgtc gttggagcga accacgtaca
     3421 tttgcttttg aaccaaaacc acactgggaa attgcagaaa atcttgatat tttagatttt
     3481 gaacgtggcg caaaagtagc cggtagccgt tttgtctatt acaaaggatt aggtgctcga
     3541 ttagaacgtg ctgtctacaa cttcatgctt gatcagcata tttacgaaca cggctatact
     3601 gaaatgatca ctccttatat cgtcaacagt aaagcgatgt ttggtacggg acaatttcca
     3661 aaattcaaag aagatgtttt ccaattggag aatactgatc taacattaat cccaacagct
     3721 gaagtaccat taactaacta ttataatgat gaaattttag atggtgcaga tttacctatt
     3781 tatttcactg cactaagccc tgcttttcgt tctgaagccg gaagtgctgg tcgggataca
     3841 cgaggactga ttcgtcttca ccaattcaat aaagtagaaa tggtcaaatt ttcggatgta
     3901 gagcattctt acgaagaatt ggaaaaaatg acagctgatg cagaagatat cttgaaaaaa
     3961 ttgcatttgc cttatcgtgt gatggcctta tcaactggag acatgggctt ttctgcagct
     4021 aaaacttatg acttagaagt atggatacct gcacaagata cttatcgtga aatcagttct
     4081 tgttctaact gtgaagattt ccaagcacgc cgcgcaatga tccgttatcg cgataatgat
     4141 ggaaaaatcc actatactca tactctgaat ggttcaggac ttgcagttgg ccgtacagta
     4201 gctgccattt tggaaaacta ccaaaatgaa gatggttctg tcacagttcc agaagcgctc
     4261 gttccttata tggggaatct gaaagttatt aaataaagag gctgtgaaaa agccgcttag
     4321 tttcgagtat taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac
     4381 ttaataccga gaaactggct tttgaacacc atttatcgag aggttgtgaa atcagcgaat
     4441 taacctgagt attagagaaa aaaatggaaa aatagtccct catattttac cgtttgttga
     4501 actaataccg agggtcagct gattgaacac cgtttagtgg agaggtcgta agcctacccc
     4561 ccagctccta gttacaagga caaattcaaa aaacagctct tcatatttta tgaatgttgt
     4621 acttgtgatt cggaggtggc aagggaacac tgtttattca aaaagaggct gggacagaag
     4681 cgtttaactc caagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat
     4741 ttcttcttat ttccgaagga gttgcttcta cttccgccgt ttatacgtgt ttagagcgtg
     4801 agacaaaagt gttctttact tttgtcccac gctctttaat gtttttaaga ttcaactgaa
     4861 tagtttggtg cttcttttgt gatttgtaca tcatgagggt gtgattcttt taatccactg
     4921 ccgctcattt ggataaattg cgcttcatca cgtaattgct gtagattggc tgccccaaca
     4981 taacccattc ctgaacgcaa accgccgatc atttggaaaa taatatctgc aacacttcct
     5041 ttataagcaa cccgaccttc gattccttct ggtactaatt tatttgcttc attgacacta
     5101 ccttggaagt aacggtcttt tgaacctttt tccatcgcgc ctaatgagcc catacctcgg
     5161 tatgttttga atcgacgtcc ttggaagatt tcgaattcac ctggtgattc atctgtccct
     5221 gccaacatac ttcctagcat cactgcatgt ccgccggcag ctaatgcttt gacgatgtca
     5281 ccagaatatt tgatccctcc gtctgcaata atcgctttgc catattgacg agcaactgat
     5341 gctgcatcat aaatagctgt gagctgaggt acacctacac cggctaccac acgtgtcgta
     5401 caaatcgatc ctggtccgat accaactttt acgacatcta cacctgcatc atataaagct
     5461 tttgtcgctt cagccgtcgc aacgtttcct gcaatcaatg ttgcatctgc aaaagttgaa
     5521 cggatttctt gaatttttcg gatcacacct gcactatgtc catgagctgt atcgatgatg
     5581 attgcatctg cccctgcttc taataaagca ttcgcacgct caaatgtatc gcttgttaca
     5641 ccaacggctg ctgctacaag caatcgacca tgttcatctt ttgctgcatt tggaaattcg
     5701 atcactttct cgatgtcttt gatcgtgatc aatccgctta aacgaccttc gttatctacg
     5761 attggtaatt tttcgatttt atgtttttgc aaaatctttt ctgcatcttt tagagaggta
     5821 cctactgggg cagtcactaa gttgtctttt gtcatcacat cagcaatcgg catgtgataa
     5881 tctgttacaa aacgcatgtc gcggtttgta attatcccta ccaattttct gttttcaagt
     5941 gtttcaacga ttggcacacc actgatacga tatttgctca tcaattcttc tgcgtctgct
     6001 acaagatgct gtggcgttaa gaagaacgga tcaatgatta cgccactttc agaacgtttg
     6061 acttttctta cttcatccgc ttgctggctg atcgtcatgt ttttatggat cacacctagt
     6121 ccgccttgtc ttgccatagc aatggccatc ttgctgtcgg taacagtatc catacttgca
     6181 ctgatgattg gaatattcaa cttaatattt ttagctaatt gtacgctcat atccacttcg
     6241 tttggtaaca cgtgactttc tgctggtatc aataagacat catcaaaagt aaatcctttt
     6301 ttcgtgaact ttgtttccca gttggacatt tttagtacca ctcctctaat ttagtttatc
     6361 tattaaaata attgattttt tcatttaagt caatgatcta cttggaatca agttagcttt
     6421 ctcatagaat accaatataa agcgtttaca tctgtgtata ttaaaagaga ttgtaaaaaa
     6481 gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgaat
     6541 tggttgggtt tattaccgca gatgcaaact tttgaacacc gtttagtgga gaggctgtga
     6601 aaaagctgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt ccacattttt
     6661 gcataaattg gacttaatac cgagaaactg gcttttgaac accgtttatc gtaaaaaggg
     6721 actgtgacaa gacttttgtc acagtccctt actattattt aactatggat aaacaaaatg
     6781 aggttcttta ctacccctag atatctagca aaatagctga tctttttgct agtttatctt
     6841 cttgggacgg ataaacttcc aataatcccg tattttttct tcaagtagta acggattgct
     6901 aaagcagttg ccccgataat gataactact actggatcta atactggatt gataactggt
     6961 ggcaatagtg ttgtcgcaga aaacagtacg acccaaagaa gtgtcgttcc acctaggata
     7021 agcaatgtct tacccattcc tggtttcttg cttcgatctg ctccgggata ttcatattga
     7081 tagatgtatt tgtacatcat ataaaagacc cagccaccga ttgcagaacc tagaacgagt
     7141 gtagaaaagc cataagctgg cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg
     7201 atacttaata gaccaagcaa cagcaacgta ttatctaacc acatcaataa aggggtggat
     7261 gaatttttta cttctgctgg cttattgatg attgcttccg ttctttcaga gactgtccca
     7321 aacaattgac gggctgttct tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca
     7381 tgaagggctt ttactttttc ttcttcagac aaattcgctg cttctaaaga ttttttcaaa
     7441 tcaaaaatgt actgttgatt tcttttggtt aattttgttt ctaaactatt gttttctttt
     7501 acgattgctc gtaattgttc tggttccatt atctaatagt cctccttagc cgttcctttt
     7561 ctcaacggtc gtcacaaaag acattctacc atattctaca gctagtttga tagccctttt
     7621 acacattaaa gcggaataac attacgtcgc cgtcttgtac gacataatct tttccttcta
     7681 atcgaacacg tccagcttct tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa
     7741 aagagacagt ttctgcacgg ataaatccgc gttcgaaatc tgaatggatg attcctgctg
     7801 cttgtggtgc tttgattcct tttttgaatg tccaagcacg tacttcttgt tccccagcag
     7861 taaagtaagt agctaagcct aacaaatcat aagcagcacg gatcaactga tccaaacctg
     7921 attcttcgat tcctaaagct tctaaaaatt ccgctttgtc ttcttcatct aattctgcga
     7981 tttcttcttc tgcacgagca ctgaccacga tcacttctgc attttcttct gcagcaaagt
     8041 tgcgcacttc ttgtacataa tgattcgcgt ctgcatctgc tacgtcatct tctgagacat
     8101 tggctacata aagaattggt tttgtcgtca ataaaaatag tgatttaacg attttttgtt
     8161 cttcttcagt aaattcgatc gttcgggcag attttccttc ttccagtaca ggtttgattt
     8221 tatccagtac agctagttct gccactgcat ctttatcttt ggttttagca acttttgcta
     8281 cacgagtata gcgtttgttg atagattcta agtctgctaa cactagttcc aagttgatcg
     8341 tatcaatatc ttctagtgga tcaacacgtc cttctacgtg agtaatatta tcatcatcaa
     8401 aacagcgaac aacgtgacaa atagcgtcta cttggcggat atggcttaag aattgatttc
     8461 ccaaaccttc tcctttactt gcgcctttta cgattccagc aatatctgta aattcaaaag
     8521 tcgtcggaac agtttttttc ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag
     8581 gtacttctac catccctacg ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg
     8641 ctcctgcttt tgtgatcgca ttaaacaaag ttgattttcc tacgttgggc aacccaacaa
     8701 ttccagcagt caatgccata aatttattca ctctttctct gcattttttt caattacttt
     8761 ttttaatttc ttttcaaaat ctcgacgggt catcatgacg atatgtccgc aattcataca
     8821 tctgattttg atatctgcgc ccattcgaat gacttcccag cgattcgtct ggcaagcgtg
     8881 aggttttttc atttcaacga tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt
     8941 tattcttcat caaattgaat attcaagata tcaagaattc gagtcaaatc tttttgagaa
     9001 agatattcaa tctcgatctt acctttccca tttttttctt ggatagctac acttgtacca
     9061 aatttatcca tcaaacgatc ttcgctttct cggatataat atggtttttc ctttgctagc
     9121 cttgggattt ttttcttttc ttttccatca ttttgattta actcagaaac aagctgttcc
     9181 agttggcgga cagttaaatt ttcagttact gcacgattgg ctaatttcaa gatcaatgtt
     9241 ttatttttta aacctaacag cgtacgggct tgtcccatag ataaacgctg atcttgtacc
     9301 atttccttga caagatccgg taaagaaagc aagcgcaaat aattggcgat ataaggtctg
     9361 ctctttccta aacgactagc tacttctgct tgtgttagtt tcaggttttt catcagcatc
     9421 tcatatgctt ctgcttcttc taacggattc agatcttctc gctgcaagtt ttccagcact
     9481 gctacttgca tcatcgcttc ttcgtcaaag tctctaacga tagcagggat cgtttctttt
     9541 tcagctagtt tggacgctcg aaaacgtcgc tcccctgcaa taatctcata tccttttact
     9601 gcggaacggc gtacgatgat gggttgaaag acacctgatt gttcaataga attagctagt
     9661 tcctgcaaag atacttcttc aaatgtttta cggggctgat acggatttgg tcgcagctca
     9721 ttcaatggta gctggacaac ggtatcatct tttacatcca ccgtctctaa gttttctaaa
     9781 tcttgaaaca gtgcatcgat tcctcggcct aatcctttac ttttcttcac gacttaccac
     9841 ttcctttgcc aatgcttggt agacttcagc tcctcgagaa cgtggatcgt agtcgatgat
     9901 cggttttcca tgactaggtg cttctgataa acgaatattt cttggaatga tcgtttcgta
     9961 aactttttcg cggaagtatt tacgtacttc ttctacgact tcatttccca gatttgttcg
    10021 tgcatcatac attgtcagta agactccctc gatctctaat tctggattaa agtgtttttg
    10081 gactaaacga accgtattca gtaattggct taatccttcc aatgcgtaat actcacactg
    10141 aacaggtatc aaaatcgaat cacttgctgt aaatgaattg attgtcagat ggccaagaga
    10201 aggcgggcaa tcgatcaaaa tataatcata ctgactgctt acttctgcta aagatccttt
    10261 taatcttgat tctcttgcca tcattgacgt caattcgatc tctgctcctg ctaattggag
    10321 agtcgcaggc acaatactca aattttcgtg ttcagtgatc aaagttgcct catcaattgg
    10381 caattcattg actagaacat catagatgtc tcttgtcaca tcaggttttc tgatcccgac
    10441 accactggta gcatttcctt gtgcatccat atccactagc aatacttttt tgccaaggct
    10501 agcaagacaa gcacctagat tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt
    10561 cgcaacagat attatttgtg ccatctcttc tcctcctatt tgattggctg cttattaggc
    10621 agtccgggct ttcttgggta tttctttggg gttggtttct ttttttgaat aacgatgata
    10681 tgccgttgat cagagacatt cggcaaggta accgcctctt ctttgaggaa tttaccacct
    10741 aataaagcaa tcgcttgttt tgcttctgtc aattcctctt cactttttgc tgctttcaat
    10801 gcataaaaat agccttcttg tttagccaag ggcaaacaca attccgctaa cacattcaaa
    10861 cgagcaaccg cacgagcagt gacaaggtca aatgatgcac gaaagtgagg attttgccca
    10921 aatgtttctg cccggtcatg atataaggaa gcatcgattc ctaataatcg ggttaactca
    10981 gataagaagg ttattcgttt attcagagaa tcaacgatcg ttatctgcag ttctggaaag
    11041 gcaattttta aaggtatgct aggaaaccct gcccccgaac caacatcgca taaagataaa
    11101 ggttgcgtaa agtctacttc aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat
    11161 acgtcttttt catccgtaat agctgtaagg ttcatctttt cgttccattc gactaatgtc
    11221 cgataataaa tagcgaattg attcatttga tgatcggtaa gctctatccc tttttctgcc
    11281 aaggattggc gaaattcttc tggtatcata aatattcctt tcattgtgaa acagcaattg
    11341 tttcacagat tctctcccta ctttaacgca aaatctctag acatgcaatc cttccatatg
    11401 gattcttaaa aatttgaaaa gtaagaaaag ccgatagact ttttctttct agtatgactc
    11461 ataaaatata cacagaagag agaactagct ttgagcaatt tctgcaaaaa ctagttctct
    11521 cttctattta ttattacgta ttacgctggt tcactgactt tcgcaatctt cccttgttcg
    11581 atatagacca ttaggatact taggtctgca gggtttacgc cactgattcg gctagcttgg
    11641 gcaattgttt ctggttggat tttctgcaat ttttgttttg cttctgttgc taatccgttg
    11701 attgctgcgt agtcgatgtt ttctggaatc tttttcgctt ccatgcgttt taatttttct
    11761 actttttcca tggctttttt gatgtagcct tcatatttga tttggatttc tacttgttcg
    11821 atttcttttc gtgtcaattc ttcattttct ggaataaacc gtagaagttc tgcataagta
    11881 atctcaggac gtcttaaaaa gtcgcttgct agtacaccat cttttaatgg tgccgcatta
    11941 atcgttgcta aaaagtcttg gacttcttct gttggtttga ttcgtacatt catcaaacgt
    12001 ttgatttctt gttcaactgc ttcttttttc ttcaaataat tcttgtattg atcttctttt
    12061 actaagccta cacggtgacc catttctgtt aatcgcaaat ctgcattatc atgacgtaaa
    12121 agcagacggt attcagcacg tgaagtcagc aaacgataag gttcgttcgt tccttttgtt
    12181 accaaatcat cgatcatgac accgatgtaa ccgtcacttc gtttgatcac taaaggttct
    12241 tttccctgaa ctttcaaagc tgcattgata ccggcaatca gcccttgtcc agccgcttct
    12301 tcataaccgc ttgtaccatt tgtttgtcca gctgtataaa gattttcaac gactttagtt
    12361 tctaatgtcg gacggagctg gtgtggctct actacgtcat attcgatagc atatcctgta
    12421 cgcatcattt ctactttttc taatccagca atagaatgaa gcatgttgac ttgaacatcc
    12481 tctggtaaag aagtagataa accttgaaca tagacttctt ccgtatttaa gccttctggt
    12541 tccaaaaata attgatgacg tggtttgtct gcaaaacgca cgatcttatc ttcgatggat
    12601 ggacaatagc gagcgcctac cccttcaacg atccctgtaa acattggggc acgatgtaag
    12661 ttgtctcgaa taatttggtg agtcgtctca cctgtatagg tcagccagca agattcctga
    12721 tctagtttat aggcactatc tggtgtgctg aagctaaaat gattcggctc tttgtcacca
    12781 ggctgctctt ctgtaacaga atagtcgatc gtacttgatt ttactcgagg cggtgttcct
    12841 gttttgaacc gttcgatagc aaagccaagt tcttttagat gattcgctag tccaatagaa
    12901 ggttgagagt tattcggacc tgatgaatat ttcaattcac cgataatgat ttctccgcga
    12961 agtgctgttc cggctgtaat aacaactgct ttggcccggt agctcgctcc tgtggacgtc
    13021 actactccat gacaaacacc atcttcgacg atcagttctt cgacgattcc ttgtcttagc
    13081 gtcaaatttg gttctttttc gatcgtatgt ttcatttctg ttgaataagc atgtttgtct
    13141 gcttgtgctc gcaatgctcg gacagctggg ccttttcctg tgttcaacat acgcatttgg
    13201 atataggttt tatcgatatt cttacccatt tctccgccta acgcatcgat ttctcgaacg
    13261 acaacgcctt tagctggtcc gcctacagaa ggattacatg gcatgaatgc taccatgtcc
    13321 aaatttattg tcagtaataa tgtttttgat cccatacgtg cagcagcaag tgcagcttct
    13381 gaaccggcgt gtcctgcgcc aacaacgatc acgtcatacg catctgcttc ataatgttcc
    13441 aatcgtttgt tcctcctttt attttcctaa acaaaattga ctgaataatt gagtgattag
    13501 ctcatcttgt acactgtcgc cgacaacttc tcctagatag tcccagcaac gcgtcatatc
    13561 tatttgaacg agatccacag gcattcctga atcaatacct gcaatgactt cttcaagtga
    13621 gagggaagct ttttctaaca acgcgatatg gcgggtatta gaaacataag tagcgtctct
    13681 ttctcctgtt tctccgctga agaataaatc agaaatagct gattccaaac gatctagtcc
    13741 gtcgttttta gctacggaaa ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt
    13801 ttcttgttcc agctgtgctg gcaaatctgt tttatttaga agaatgatcc gtttcaatcc
    13861 acttgtcgct tccaataatt gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac
    13921 aagtaagatc aagtctgctt ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc
    13981 caccacatct tctgtttccc gaattccagc tgtatcgatt agcttgagcg gtaccccccg
    14041 aacattgaca tactcttcga ttacatctcg agtcgttcct gcaatatccg taacgattgc
    14101 tttttcttct cttaacaaat ggtttaacaa acttgatttg ccgacatttg gccggccgat
    14161 gattgccgta cttaaccctt cccgcagaac cttcccttgc ttggatgtag caagtagcgc
    14221 ttggattctt tgttggatca tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc
    14281 gacatcatca tattctggat aatcaatatt tacttctact tgtgctaatg tctctaaaat
    14341 ctcttgtctc aatgagcgaa tcaaagcaga cagattgccg tctaactgat taagagctag
    14401 tcccattgct ttgtctgttt ttgcccgaat caaatccatc acagcttctg cctgtgaaag
    14461 gtcaacacga ccattcaaga atgctcgttt tgtgaattcg cctggctcag ctaatcttgc
    14521 accttctctt aacaatagct gcaaaatttg attgactaca acgatcccac cgtgacaatt
    14581 gatttctact acatcttccc gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac
    14641 ttcatcgaca agctgattgc tcttgggatc aacgatatgt ccataatgga tcgtatgact
    14701 tggtacttct gataatcgtt tgtttccaga ttgataaact ttatctgcta gttgcacagc
    14761 ttgatcgcca cttaaacgaa cgatactgat tgcaccttct ccaggtgggg tagaaattgc
    14821 cgcaatcgta tcaaattcta aagtaatttc agccattttt tcacttcctt tttcattttg
    14881 cgcacaaaaa aagtgcccac tccacccttt atgcacaata aacggtgaac ttcgcacttt
    14941 gactgcttgt tcttgattaa attctggtca tagattatca cacttgctat aaggaatcaa
    15001 gtgtttaaaa caattagatg aagttgagcc tgttctgctt ccattacctt atatttaatt
    15061 gtccttggat gtctctttgc gttctttggt ttattcgaag agaagatact gtataaacaa
    15121 ggctttatct ttcttaaaaa aaacggtaca ctaaatggta gcgctatcta aactatacac
    15181 ttttgaaagg agaaaatatg tgagcccgtt cagatctgct gcattggaag aagaaagtga
    15241 ccctcggatc aacgatgtcg ttttatctac agggaatatc aaccagaaat atatcgtccg
    15301 cgatatcgtt tttgttgtgg aatgcttaga agctgatttg tttgacccat cttttcccaa
    15361 agatgacctt ttcttaacaa cgaagaggaa attaaagacg aaggtacttg aatatggtgc
    15421 gaatgcagta atcaactgtc attttgagta tcagcaaaaa aaacgcgacg gaaagtattt
    15481 tctggaactt tttgcttatg gaacagtgat ccaatttatc caaacaacga ttggttaaat
    15541 gaaacagaca aaaaaagcgg atcaaaactg aagacagttt cgatccgctt ttttctatca
    15601 aagttagtga ctacttcatc gtatactctg ataagaatga tttctagtaa aatatacttg
    15661 gtttgaaaaa ctttgtcaac gtgatttatt caaaaagtaa tggaatcaat aatatttttt
    15721 agcaggttct actactaaat aacgataagg ttcctcacct tccgaatgtg tttttacatg
    15781 ttcatctttg cttaatgcag catggatttg tttacgttca aatgcaggca ttggttctaa
    15841 gaaaacaggc tgccctgtcc gttttacttt ttcagccgtg cgatcagcta aacgctgaat
    15901 gatctcttgg cgcttttcac ggtaattgcc tacattgact actacagaca gcttatttct
    15961 tgctaaacga tggatgaaga cttgtgccag atattgcaat gcattcagtg tttttccatg
    16021 tttcccaatc agtatgcctt gcttttgtgt ttctaaatgg aaaacaacca tattatttga
    16081 ttctctttcc attttgacaa gagctggagc atcaagctgt tttgaaattt cagtaagata
    16141 aactgccaat tccgttaaag ctgcttcatc acctagctca cttacttctt ctgttttttc
    16201 ttcaagaatc gtttcgttga ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc
    16261 ttctacagtc tgttctactg tttcagaaac tttttcgctg atacttggct ggatcgaaag
    16321 ccgtgcattc tttcgaccca ttcctaggaa accttttttt ccttcttcca gtatttcgat
    16381 ttcgacttgg tctttggcca aacccaaatc agccagacct agttggacag cttcgtctat
    16441 tgttgcagct tcataaatcg gcattagcat tacctcccct attatttccg ttttttctta
    16501 ggattcattg ctttcttaag agcacgttct ctttcgcgga ttctccgtgc ttcttcttca
    16561 cgctcttttc gaattttgaa agggttgttg atcagcagcg tttgtacaac ttggaaggca
    16621 ttggaaacta cccagtataa tgaaagaccg ctggctaaat tgacacccat cactaaaatc
    16681 attacaggca tcacgaaatt catgatcttc attgatgcat tcgattcaat ttgactcatg
    16741 ctagataaat acgtacttgc aaaagtaaag attgctgcta aaatcggcaa aataaatgtt
    16801 ggatccgcgt tccctaagtt caaccataag aaatgccctt gtttcaactc tggtacacgt
    16861 gaaatagatt gccataacgc catcaagatt ggcatttgta ctaataaagg aagacaacca
    16921 gcatatggat tgacgccatt ttctgcatat aaacgctgct gctcttctct taacttactt
    16981 tgtgtttccg gatctcttga actatattgt tgctgcagtg cttttaattt cggttgtaat
    17041 tcttgcgttt tacgcatgct ctttgtttgg aaatgcatca acggcaatag gatcacgcgg
    17101 atgatcagtg taaataaaat gatccctatc cctgcattac caaatgacaa cgccttgatc
    17161 gcctcagcaa agtaataaac aatataacga tcccaaattc ctgtactctg tgcgctgact
    17221 tccccagttc cgcaaccgga taagacgatg accaaactaa cgataccggc catcaaaagt
    17281 aaacgtttgt atttcttcac ttactctttt cctctctgtc gattattttt gcaagtttta
    17341 agacgtggac taaattggag cgaacctcat cataagtcaa ccccttcaca cttggtctag
    17401 caatcacaat aaaatccaac tccggatcaa tttcttcttt tatagaataa actgctgctc
    17461 ggatttttcg tttgacttca ttgcggttta ccgcgttgcc gacctttttt cctacagata
    17521 aaccaactcg aaaatgaggc tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt
    17581 tagcaaaaga atctcccgta tggaacacgg cttgaaactc tcgttctttt tttactcgat
    17641 acgcttttct cattagtttt ctttcctttt ctatctctca cttacgctca taatcatacc
    17701 ataattacga ttgtcctttc atcatttccc taaaaaagag tataaaaaaa accactgaaa
    17761 ccccagtggc ctaagctgaa agaacttttc ttcctttgcg acgacggcta gctaacacac
    17821 gacgtccatt tttagtactc atacgtttac ggaatccgtg aactttttga cgtttacgtt
    17881 tatttggttg gtatgttctt ttcatatatt ccacctccaa aatgttctta tctattttat
    17941 aaacacagac atactttgat agtataacga caaagtagtg attttgtcaa tataaaatag
    18001 aagatcgatt gttaaaactt atccacaaga ttattttttt ctatgggaat cctatgtgga
    18061 aaaagttatg cacagggatt atccacagat aataaaaggc tggttttaca cagaaaacac
    18121 acgtgtaaaa aaagtaatcc acaatcaggc gatttcctgt gtattccgtc tcctaactat
    18181 tggtatatct gtatttttta tccacagacg caagaggaaa tctattggtt tttacgagtt
    18241 ttccacctgt gcattagtgg tgtggaaaac tttttttgac aactgtgttt ttctcttttt
    18301 tctttttgtc gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg
    18361 aaaactattg aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag
    18421 gccttatatg gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga
    18481 tttatcgcct gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca
    18541 aaaccagctc gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct
    18601 ggcgacaaaa atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt
    18661 tattactggc gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt
    18721 ggcacctagt ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt
    18781 cattggtaaa ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg
    18841 ttctatttat aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat
    18901 gcatgcaatt ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag
    18961 cagtgaaact tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt
    19021 ccgtcaagaa tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga
    19081 aaaagaagct acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa
    19141 acaaatcgta ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct
    19201 tgtttctcgc tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag
    19261 aacagcgatt ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact
    19321 aagctacatc gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg
    19381 agtgcaagct tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc
    19441 actgaaaacg ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga
    19501 agaagtggca aagtattatc acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa
    19561 atctattgtt gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc
    19621 tttaccgaaa atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca
    19681 tgaaaaaatc cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat
    19741 caaaaacttt ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa
    19801 aagaaaaaaa cgttcaaaag ttatacacac tttttccaca gctgtttttt cgtttctctc
    19861 ttacgttttt tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta
    19921 actaataata aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga
    19981 gctagcttta tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc
    20041 cctattttga caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc
    20101 aacgctgata tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa
    20161 atcgaatcta ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga
    20221 cttcctgaag aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct
    20281 ggaaaagcga attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt
    20341 gtcgaaagcc ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa
    20401 acagtttttg ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt
    20461 ttatctgata attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg
    20521 attccagtag aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc
    20581 gaattatctc gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac
    20641 caagtgttgt tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat
    20701 ccagatacca atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct
    20761 agctttttag cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc
    20821 gttcgtttat ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg
    20881 aaagtagaag aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc
    20941 aatccggatt atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa
    21001 tttatctctg ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt
    21061 cagctgatta ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa
    21121 ttgaaaagaa atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc
    21181 tttctgttta tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat
    21241 atatggataa atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat
    21301 gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg
    21361 ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg
    21421 gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat
    21481 cccggcatga tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac
    21541 gacgataatg aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa
    21601 catcgaattt tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa
    21661 tcttttggaa agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca
    21721 ggaattgatt ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc
    21781 atccactctt ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca
    21841 tatcgaacag aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc
    21901 agaagatctt tctttagtaa aaggcagccc gcagaccagg cggaagtttt tagatatgga
    21961 attaggccag atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa
    22021 acaaagaaac cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt
    22081 ggatattttg tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca
    22141 gttcgtcaag cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa
    22201 agagcagctg gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga
    22261 gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg
    22321 gggaacaacg acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa
    22381 tgtgcagact tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc
    22441 agaaatcgat ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt
    22501 gatgagcgag ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt
    22561 tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc
    22621 tgagattttt tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa
    22681 agaagtttag tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga
    22741 cttgaagccg ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg
    22801 catcaccttg tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca
    22861 acgaaaatcc atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg
    22921 gggatacctg tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact
    22981 gttttacatg ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac
    23041 ggagtaggat cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa
    23101 gaaggaaatg tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt
    23161 atcgaacaaa cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt
    23221 acagagacaa ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc
    23281 ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat
    23341 gaataccatt atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc
    23401 gtgatttttc cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc
    23461 tctatgcaat atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat
    23521 acgtatgaag gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat
    23581 gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac
    23641 gtgcgagaag gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga
    23701 caaacaaaaa caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc
    23761 gaacatttca tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa
    23821 ggattactag cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt
    23881 aaaggtgctt tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat
    23941 cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa
    24001 ggtcgtaatc gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa
    24061 aaagcaagca tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg
    24121 ggtacagggt ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc
    24181 atgactgatg ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat
    24241 cgttacatgc gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac
    24301 ggggtaaaac aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag
    24361 ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa
    24421 ggacttggag aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg
    24481 atgatggccc gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg
    24541 atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag
    24601 aacttagata tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat
    24661 ccatgatgtc aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt
    24721 tattgtcgca cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat
    24781 tttatacggc atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag
    24841 aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc
    24901 aatggtacgg atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa
    24961 ctttggttct gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag
    25021 caaaatcgct acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa
    25081 ctatgatgat acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt
    25141 gaacggaaca acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc
    25201 tgaggtagta gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt
    25261 gatggaagta ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg
    25321 aatccgaaga gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat
    25381 tactgagatg ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa
    25441 taaagcaaaa ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat
    25501 cacagatttg cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg
    25561 tgacgccagc gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc
    25621 atttggattc aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca
    25681 aattcttgaa aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga
    25741 aaaagaaaaa gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca
    25801 tatcgatgaa atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac
    25861 gatgatcgaa cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt
    25921 acgccgattg accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa
    25981 actgattgaa gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa
    26041 gaatgaatta gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt
    26101 cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac
    26161 attgacaaac aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag
    26221 gggtggacgt ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt
    26281 ttcttgttct acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc
    26341 aaaaggatac gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt
    26401 actaggtatc gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga
    26461 agaaggccac tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac
    26521 tgcttttgct aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga
    26581 gctcgtcaat gtgctactga cagatggtca atctaatatc attattggaa cacataacgg
    26641 ctactctgtc actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt
    26701 tagaggaata cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga
    26761 tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata
    26821 tccagtgaaa ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg
    26881 tccactagct ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa
    26941 aggtgtcatt atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg
    27001 cgtacgtttg atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc
    27061 agaagctgca gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat
    27121 tgtgtaagct agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga
    27181 ttagttgaat ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa
    27241 tagagtgtat aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt
    27301 aggaacctaa gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg
    27361 aaatcatgta cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac
    27421 gtttcgatgc aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa
    27481 aacgccgttt cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag
    27541 tatcttctcc atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg
    27601 acgatatcat tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac
    27661 aatagaaaac gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt
    27721 ctgacgaaag atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact
    27781 ttggctgtga atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac
    27841 tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt
    27901 ttaggtgtta ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta
    27961 tacgtgacag aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa
    28021 aatcgccaac aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga
    28081 aacaataact ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat
    28141 acttctgacc cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc
    28201 taaacgaatt tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg
    28261 tcgtaaagta gactacattg ctgctaacca tattgaatat atcgattata aagatatcga
    28321 attactaaaa agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac
    28381 aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt
    28441 gctacctttc gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat
    28501 cagctattca attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg
    28561 acgaaagtta tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa
    28621 acaatcattt atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg
    28681 tagtcttaat tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat
    28741 ggggtgttcg ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa
    28801 tccagcaggc ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg
    28861 taggagtgat tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat
    28921 tgaaaatcaa agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg
    28981 caaaagaaaa aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg
    29041 atacagtaaa gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc
    29101 atcagacgaa cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca
    29161 ttgataaatt ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt
    29221 ctgactggat gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct
    29281 tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc
    29341 gctttcgagc agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt
    29401 atggctcaag aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt
    29461 tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt
    29521 attatggggg gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga
    29581 gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt
    29641 ttccggatat ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc
    29701 agaaaaaagc ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc
    29761 ttgaagaaat ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca
    29821 tagagctgaa aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat
    29881 cgatctctca gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc
    29941 gtggggaaga gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag
    30001 cttcagagct agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc
    30061 gttttgaggc cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc
    30121 gtacaactgc acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat
    30181 ccttagtaca atatttattg agctcggatt tagcgacata cttggagatc agcaatttga
    30241 ttgctggaag cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag
    30301 agtataatag tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca
    30361 atgcagcttt cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta
    30421 ccggcacgat caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa
    30481 acgcagctac tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg
    30541 caatcaaaga aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt
    30601 tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta
    30661 tgcacaaaat tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag
    30721 tcagttagca ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc
    30781 taaaaagtta aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc
    30841 aggggaagac agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa
    30901 caaacaatac aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc
    30961 actaggctac acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt
    31021 tcatgtcgta caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga
    31081 aggaaaaaag acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc
    31141 tgcaaatcga tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag
    31201 aaaacgagta taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact
    31261 tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga
    31321 agtgtggcag gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc
    31381 tgtttttctt gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt
    31441 ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga
    31501 agcgattgac gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc
    31561 cggtggttta agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt
    31621 ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac
    31681 aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc
    31741 cgaacgcagt atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc
    31801 tgaagtactg aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat
    31861 cacaggtcta ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga
    31921 agaactaatc attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat
    31981 tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg
    32041 ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt
    32101 acgtacaggt cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct
    32161 ttctcgagca aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc
    32221 gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt
    32281 gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg
    32341 ccaactgaaa aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc
    32401 tcgtggagta gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg
    32461 atcaattgaa caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag
    32521 agaaggcgga gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat
    32581 catcgagaag aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata
    32641 caataagttt tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg
    32701 agcaatcccg agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta
    32761 cttaatttaa acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc
    32821 cgaataaacg gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg
    32881 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct
    32941 cttttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa
    33001 catgagaagc agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca
    33061 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga
    33121 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    33181 cacaacctct gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc
    33241 acaaaaattt gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc
    33301 tgttacaacc tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt
    33361 ttataaaata aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac
    33421 aatggttcag gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta
    33481 ggtacgcaat ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct
    33541 gaagtgattg cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt
    33601 gtgacttata aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg
    33661 atcggaaacg gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat
    33721 gaaaaaggtg tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct
    33781 tatcatatca aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca
    33841 acgattaaag gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt
    33901 gcagacttat tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa
    33961 aaccgtcaat ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa
    34021 gaatattatg aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg
    34081 aatgatgctt tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg
    34141 gatatcgacc agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta
    34201 acaatcggaa gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc
    34261 tatacgtcac gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa
    34321 caaatccgcg aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc
    34381 tggtttgatt ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct
    34441 ttaaactcga tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa
    34501 ttagatggcg aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa
    34561 ccaatctatg aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca
    34621 gaactgccag aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt
    34681 atctcaacat tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg
    34741 gcgcaaatct aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga
    34801 agtgtttatc tatacgataa taataacttg aacactgttt attcagaata agggactgtg
    34861 acaaaaaccg tgtcacagtc cctttttccg aacaaacggt gttcaaaagc ttgcatctac
    34921 ggcactaagc ccaatcagat gaaaaatatg gagtgctatt tttatctgat tgttcttact
    34981 gcttgatgca gagcagcttt ttcacaacct ctccactaaa cggtgttctc ctgccaactc
    35041 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gttttttgaa attgttcctt
    35101 gtgactcgga gctaaacggc aggctcacaa cctcttggta aacggtgttc aatcagctga
    35161 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttttcc gtttttttct
    35221 ctaatactca ggtcaaaacg ctgatttcac aacctctgat tcacggtgtt ccagaagtag
    35281 cttctacgga aataagccga aatttcacaa aaatttgaga agcaattttc gtaaattcct
    35341 tcttatttct tgaagctgac cacttctgtc acaacctctt attttgatta gaacttgcga
    35401 aaataaaccg gttaaagtgt gtctagcgca agagatttgc tataatagac gataagggta
    35461 aaatgtaacg agaaggggtt gaaaaatgtg tatcaaattg taacagattc ttgttgtgat
    35521 ctgccatacc atgtattagc aaaagaaaaa gttgattttt tatcaatgaa tctccaattg
    35581 aatgatcaag agttagtaga tgacctcgga aagacattca attatgaccg ctacatgaat
    35641 cagctgaaaa atggtgcgat gcctacgaca tcacaaatca atgttggccg atatttagaa
    35701 tttttccgtt cttatgcgga aaaacagatt cctgttttat atgtggcttt ttccagtgga
    35761 ttaagcggct cttacagtag tgcgcttcaa gcggttgaat tgctgaagga agaatatgaa
    35821 gaccctgaga tctatgtttt tgatacaaaa gctgcaagta ttggggaagg gcttttagta
    35881 ttggaagctg taagattgaa aaaagaaggt aaaagcatcg aagaaaccct agagtggctg
    35941 gaaagtaatt atttgaaagt ccattcatgg gtaacagtag acgatctgaa atatctagaa
    36001 cgaggcggta ggatttccaa agcaagtgct actcttggca gtctattgag tgtaaagcct
    36061 attattacgg tggataaaga aggaaggctg acgaatgttg acaaagttcg aggaagaaac
    36121 aagtcgatcc agacaatcgt caatgagacg gtaaaaacaa tcgttgcacc acttgatcaa
    36181 atcgtttatg tggcttacgc tggggatcta gagagcgcgg agaaggcaaa acggcttttg
    36241 gaagagcaga taaagatgaa agatgtgaag ctttatccct taggaccaac gatcgccagt
    36301 cacacaggct atggctgtat cgcgattttt tcgatgggaa tcagtcgata aaagcagtaa
    36361 ctcattctgt aaaaaagccc agaaattgat tgtaacaaaa acagcgactc ttaaaagata
    36421 gatgagcatc ctatctctta gaggtcgctg tttttttatt ttttaagcta aataggccat
    36481 tatgcccata actcgttcaa ttgtttttga atatctgcat tttccaaaaa ttcatcatat
    36541 gtcgtatcag ctcgatcgat cacaccttta tccgaaacgg caatgatgcg gtttgccaat
    36601 gtttggataa attgatggtc atgtgacgca aataagattg agccagtaaa tgccatcaag
    36661 ccgtcattca atgctgtgat tgattctaag tctaaatggt tcgttggatc atctaataca
    36721 agtacgttag ctttcgaaag catcattttt gaaagcatgc aacggacttt ctctccacca
    36781 gataaaacat tcactggttt caatacttct tcaccagaaa acaacattct tcctaagaag
    36841 cttcttaaga aggtgttatc atcttcttct ttacttgcat attgacgcaa ccagtctaaa
    36901 atcgttagat cagtatcgaa ttctttcgtt gtatcctttg gtaaataaga attacttgtt
    36961 gtcacacccc aacgtactgt tccagtatct ggcgtaattt cacccatgat aactttgaat
    37021 agcgttgttg ttgttatatc attttctgcg acgaaagcga ccttgtcttc tttgctcaaa
    37081 ttaaaagata tatcatctaa aattttcttt ccatcaatcg ttacacttac attttctacc
    37141 tgcaataagt cgtttccaat ctcacgttca ggtttgaatc caacgaatgg ataacgtcta
    37201 gaagaaggtt ggatatcgtc taaagtaatt ttttcaagca tttttttacg tgaagttgct
    37261 tgttttgatt ttgatgcatt cgcactaaag cgggcaataa attcttggag ttctttgatt
    37321 tgttcttctt ttttggcatt tgcgtttgct tgaagtttag ccgctagttg actagattcc
    37381 aaccaaaaat cataatttcc tacataaagt ttgattttac caaaatctag atccgccata
    37441 tgtgtacata ctttgttcaa aaagtgacgg tcatgggata caacgatcac agtattttca
    37501 aaattgatca aaaattcttc cagccaattg atcgattgga tatccagacc gtttgttggt
    37561 tcgtctagca aaagaacatc aggtttacca aatagtgttt gtgctaataa cacttttacc
    37621 ttttgtccac cagttaattc actcatttgc tgatggtgca attcttcagg gatgttcaga
    37681 ccttgcagta aaacagctgc ttctggttca gcttcccatc cattaagctc agcaaattct
    37741 ccttctaatt cagctgctcg aataccatct tcgtcagtga aatcctcttt catgtaaatc
    37801 gcatcttttt ctttcagcac ttcatacaga cgcttgtgtc ccatgatgac tgtttcaagt
    37861 acagtatagt cttcatagtc gtaatggttc tgtttcaata cggccatccg ttcatctggt
    37921 cccatagtaa cggaacctgt ggaaggctgc agatcccctg ataacacttt aaggaacgtt
    37981 gatttacctg caccatttgc accaatcaag ccatagcagt ttccgggggt aaatttaata
    38041 ttcacatcat caaatagttt gcggtctgaa aagtgcaaac tcacatcatt tacagtaatc
    38101 aatttttttc ctcactttta tcattattag ttttgacaag tcttatagaa ttatataggg
    38161 gaaaggtaaa agattcaagg caatcgaaca ttctttgtgt ttcacgtgaa acagaatgaa
    38221 aaaaataaaa ataaaagcgg agcgaaaata gctactatag gatttattag tttgcaacag
    38281 cataattttt gacgttattt ataagaggga gtactataaa actgtaaaag aaatgaaaga
    38341 aggattgaaa atggatgaaa aaagaaccat cgactggggc tcattgattt tgggaatttt
    38401 atttgtactt gtctcattgt tatcatttca agaccctgtc ggaaatttag ttgcaattgt
    38461 agtagtcttt gcgatttttg cctttattaa agggatattt gaactgtttg tacggaacag
    38521 attaaaagag ctaacaggat ataaaggaaa gatgccctta ataatcggga tcatcgatat
    38581 tctagttggc gtctttttct tattcaatat tggtgcagga gtcgcagcat tgccttttgt
    38641 ttttgcagta tggtttattg ctgattcagt tttagcgtta tttactgctg acttggcaag
    38701 aggagtaagt gaaggatatt attggtttac gatcatcgtc aatattttag gaattttatt
    38761 aggaattttc ttattgttta atccaatttc ttctgcgttg acattaagct ttttagttgg
    38821 attctatttt atgttatttg gtatcacaca tatcgtctat gcatttagat aagtaaaatc
    38881 aaacaaataa aaaagccggc ttccgattac agttttactg ttgtaattgg aagccggctt
    38941 ttttatcatg gacaaatatg cacgaggtga tttttaggtg tcgagacaaa tttatgcaaa
    39001 aatgtgagaa gctatataca caaattttat cttaatactc gaagcatagc gcagttgtca
    39061 caacctcttt tcatataatt cattcaatac tggcaaatca tggacttcat atttttcttt
    39121 gaatgcttgg atttcttctt cagaatattt tcttggatct aaatgcagat cttctactgg
    39181 caatgggcgc ttgttattga tcttcacatc gattactaca ggacgatctg attttgcagc
    39241 tgcatcaaag gcaaatcgaa gttgcgagta ttctgtgact gtaaagctat ttgccccgag
    39301 cgcttctcca actttaccga aatctgcatc ttctaaaaat acaccaaatt tcttttgttc
    39361 cgtatcttct tgttctgctt cgatgaaacc aaaagaatca ttcgaaaaga caacattaat
    39421 aattggaagc tgatatttga cttgtgtaat gatatcttgc atgaccatag caaacccgcc
    39481 atcaccactt aaggtaaata cttgtttttc tgggtagctt agttgtgcgg caattcctcc
    39541 tggcacacca ttacccatag ttgcgaacca tccagatgtc gtatgttttt gtttaccgtt
    39601 catattcaat aaacgaatag aatggatagt cgtgttaccg acatctgtga cgaaaatcgc
    39661 atcgtcttca gcaatacgat tgatttcttt atagacagcc tctggacgaa tcggttcttc
    39721 ctctcgatct tcaaaagatt tcaaccaatt tacccatttt tctttatttt cttgatttgc
    39781 taggtaccag ctatcagcag ggcgagcatc ccctaactcg acaagtcttt gcaaagctgt
    39841 gtttgcatca ccgaggatcg acaaagatgt ttcatggcgt cgtccaaatt ttgacgcatc
    39901 gatatcgatt tggataaatt cagcagttgg attaaaaaat gcacgtccaa atgggaagtc
    39961 gctaccgaca aataagatca gatcggcttc tgccaatgct tcatttccgg gtttagtagc
    40021 gacacgtcca gcaaaaccaa gaaagttttc ataatcatca ggaataattc cttttgctag
    40081 aacggaagcg acaacaggaa ctgaaaaatg ttctgagaat tctttgattt gcggaaatcc
    40141 gtttcgcgtt ccttgaccaa cataaagaac tggttttttt gctttttcaa gataaggcaa
    40201 agcagctagc aaatcttttt cttctggtaa tatcacactt gttttgtgag tgtgagcggt
    40261 ggagaaaggt tgttcgtcaa tttcttcaaa accaagatca acagggatag tcacgatagc
    40321 cacgccttta tgctcatagg cggctttaat ggcttcatct actacatgcg gaagactttg
    40381 tggtgtcatg actgtacgat tgtatacgct aacatctgcg aaaataggat tttcatttag
    40441 ttcctggaaa gagttatagt tcatggatgt agaagcaact tgtcctaaga acgctactag
    40501 aggcacatgg tccatctgtg catcatacaa accattgatc aaatgtgtcg caccaggtcc
    40561 tgcagagccg aagacagcgc cgactttccc cgttaatttt gcatctgcag cggctgcgag
    40621 tgcgccgact tcttcatgac gaacttgaat gtagttgatt tcggtttttt cttgatatag
    40681 cgcatccata attgaattga aagatcctcc gggaatgccg taaatatggt cgatacccca
    40741 gctttcaaga acttttacca ttgcgattcc tgcattgatt tttgacatgg tgtttcactc
    40801 ctttagcaat gatgatacat aaattttttg tatctagagt atagaacttt ttatcgaaaa
    40861 tcccaactaa ttcgtctttt cacaaaaatg tagtcatgtt tttgggaata tgctagagta
    40921 gtacatagat agattcaaga gaggggaatg gcatggttga atggctattg agacttgaac
    40981 cttggcagca agcacttgct gggacagctt ttacttattt tatgacagct cttggtgccg
    41041 ggttggtttt tttctttaaa gaaatcaaaa aagaagtgct aaacttgatg ttaggatttg
    41101 cttctggtgt catgattgct gcaagttttt ggtctttgtt agatccggct attacaaaag
    41161 cagaagaaaa tggagatatt gcttggctag ttgtaagtat tgggtttggt ttaggcggtg
    41221 tgtttttata tatggcagat aaaacactgc cccatatgca ttttggacca caacatgaag
    41281 cagaaggatt gcctacccat ttaaaacgga cgatcttgct tgttttttct attacattgc
    41341 ataacattcc tgaaggttta gccgtggggg tagcattcgg tgcagcagca actgcggata
    41401 atccgacagc cgctatatta gccgctgttt cggtggcgct aggtattggt atccaaaact
    41461 tcccagaagg agcagctgta tcaatcccgt tgcgacaaga aggtctgagt cgtaaaaagg
    41521 cctttgttta cggacaagct tctggaatcg ttgaaccaat tgctggagtg attggagcgc
    41581 tactagtaac gaaagtggag ctgctgcttc cttatgcact tgcttttgcc gcaggagcaa
    41641 tgatttatgt cgtggtggaa gaattgatcc ctgaagcaca gcagacattg tccagcaaac
    41701 gtcattttgc agtttttggc gtcatgagtg gatttatcat tatgatgatt ttggatgtgg
    41761 cattaggata aataaaaaaa atatatatga tatatcaaat agggccgcga acttatatcg
    41821 cgaccctatt tttttagtaa gttatatcat aatgaagtga atggtatcat acattaataa
    41881 tagttttttc tatgtgtatt tcctttgatc cagcgaacaa gccagatgat aagtgcgatt
    41941 gggattagaa ctttgaccgc aaaccataaa agcccgctta ggacactgac aaaaagactg
    42001 atgacgaagc tagctgcaat tagcactaaa agaaccatga agatatttaa gaacatattt
    42061 ttttgacgca tacaaaaccc tcctcttgtt cattctttac tatagaaaaa gtataacagg
    42121 gattgtttaa aaacacctcc aactgaagac tgatttttac ttcatctact taaatgatca
    42181 aacaattgct ttttctctgg tttatcatct aaaatagagc ggaaaaggag gagaatatca
    42241 tgcctaaaaa tcgtcttgaa gcatttacag atgctgtaat cgcgattatc atgactattt
    42301 tagttttaga acttcatgaa cctaacggag acacgtggga ggcgctcgct gcattaggtt
    42361 accgattttt tatctatatt gtcagtttta tcattttagc gatttattgg aacaatcatc
    42421 atcatttatt tcaagttgta cataagataa atggacgggt cttatgggcg aacagtttct
    42481 ttgtttttgc gctttcgctt tttccgtttg caacttcatg gatcagtgag tacatcaata
    42541 gcctggctcc agaaatgact tatggttttg ttaccttgac cgctaatatt gcgtattata
    42601 tcttggcaaa agaacttgtc aaagcaaata aaggccacga gaaagcattg aaggcgatgt
    42661 tgaataatta caagaaatcg tatctatcga ttggtttgaa tgtgttagga attgtcttag
    42721 ggtatttagt agcgccagta gccgtgctga tcgcaaacat cgtgattcta gctgcttgga
    42781 tcatcccaag tcgtaccatc gaatcacaat atcggtaaaa tgatccgtgt ttaaaattta
    42841 agagaaaaat atagaaaaaa taaataaacg atcaaatatc cgagtgatac tagtattcaa
    42901 agagggatgc agatggatca aaaaagaatt catgtaggac tgattactag ttttgtcacg
    42961 atgcttattt atttggatca aggtcatttg acttggacag tagttggtgt cgtaggattt
    43021 ttgacattat tcacagcaat aaaaaaataa acatctttat gtaatctgat gttctatcta
    43081 tttaagaaaa gcatgtttac agcagtatga ttctgttgta aacgtgtttt tatttgtgga
    43141 aaatttaatt tttagaaaag tgtatcgaaa aatagatagt tagtttttcc acagtggata
    43201 acttcgtaag agataaaaat aaaaaattag tcaaagaagg gcgtatagat ttcttttgat
    43261 aggagatata caacagaaat gaataaatat taacgtgttt tcaaacagta taaaacggtg
    43321 tggtttttgg aataaaaaaa taacatatct cattttacgc ttttgttatt ttatacttaa
    43381 aaaataacga cacagaaaat accgtgtctc agatttctct actacttcta aaaactactg
    43441 tttttttcct ttgaaatgcc ttcaaaaagt aggatcaggc agaatatact ggcagaatca
    43501 aggagaaggg agcctaaata aggagaaaaa ccatagaaaa tataaagtaa ttacttataa
    43561 aacaagcttt ttatagaaaa ataatatacc tgagtagggt ataaattaag acggaattcc
    43621 tttctagatt gagatattga ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa
    43681 aaaacccttt ttgttaaata aggggaatat agcctgtcag tttcaaactg caagtcgtcc
    43741 tgctgcaata atatagtaga ttagagatga aaatagattt ttttaaacca atgactgaat
    43801 aaaaagaccg tcatagatac acattggaat gtgtctatta cggtcttttt ttacgaacga
    43861 ttttattgaa gacggacagt tcgggtcaaa aataaaaaag ctacgatcag aaataagaat
    43921 aaaatgtatc ccaaaataat ggctagacgg tctaacttat ttgtcgatag atgtttgttt
    43981 agaagttgat aggcccataa acctatcagt cctcctgttg tattagttat caaatcagtg
    44041 atgtccgcta cgccaatacc gaataggaat tgaatcaatt ctgccaagaa gcttacaagg
    44101 aaaataatcc ctaatttttt agaaaaagaa agtctcttga aattgacatt caaaagcacc
    44161 ccaaaaggaa aaaagacaat caagttataa gtggtctcac gtaataccgt cgtattttca
    44221 aaagaaaaag tagaaaaagg gacgagattg acattgctgt aagaataagt gagaaattgt
    44281 ggaagattaa atgatagctt gaataaaatc aaccataata gaaagaataa ataaatccct
    44341 aataaaaatt ttgatacata ccgcatagac atctcctcca tttaaaaagt ataccatcga
    44401 aaaccgctga aaaatcataa aaaagtatga agtttaagga ccaacgtagt tatcgtacga
    44461 aaattagaag cttttttttg ttgggaatcc ggtttcttta acaaagctaa gatagcgagt
    44521 tgcttggaaa agttgctatc ttttttctgt caaaacgact cggaatgttc gttttttgtt
    44581 ctaatttctc caagttgttt actttttgat actaagttta tgaaaaatca agaaatagtt
    44641 gttatactag gtaatgctga caaacgggat tggcatttca tcttggttca gctttttttc
    44701 caattactag gaaggaggga tagattgttc aagacttatt ttcgtttcaa taaaatacaa
    44761 gataatcctt ttcgtatcat gaatctgatg ctacttatct ttattgtatt atggttaggt
    44821 tacataaatc atgatttttt gatttcaagt ttttcatcat tagggatctt tacttttttc
    44881 tattatcaaa acataccgat gaaatatttg ctgaaacgta tgtttctgat cggttgcgga
    44941 ctgttggttg ctttcgtttt ggggatctta tcgacttatg ttttatggct tgaaccattt
    45001 gccgtagctt tggttgcttt ttccagcaga ttcgtgttgc gattattcca tatttctaaa
    45061 ccaggtggct tgttttttgc gatgctttct gccatgggaa cgagcatgca gctgccaatc
    45121 gctcaattac cgatcgtctc actttatttt tttatgggtg tcgtttttgc cctgattgcg
    45181 gcagtgatta caaaattact ggattcacgg cctgagcaga caattgaaaa agcaacttta
    45241 aaagaaagat ttcacgaaga gcctttagtg atcattgatt ctgttttcta cagcgctgcc
    45301 ttatttcttt ccgtttatgt cagtcacggc ttgaatctgc ataatcctta ttggttgacc
    45361 ttgtcatgtg catcgatttt gctagcagaa aacttggatg cgatgaagca tcgacaagtc
    45421 caatatctga ttggatcgat gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg
    45481 tttacgcagt tgcagaccat ttttctgatc acttttctat atggaatcgc acaatttcta
    45541 gttgctagaa attatgcagt tgctaatatt tttcttaatc cgatggcgtt gatgctatct
    45601 acacttattc gtggagcata cttgatttct ttgatcgaat atcgtttttt agggatcgtc
    45661 ataggaagtt tcatcggttt aggtgttgcg tgggtaatga cggttggttt gcaacattat
    45721 ttaacggttg ttcgtcaaaa attagaatag aaggacaaat atatgtgcca aaagaggtta
    45781 tgacaagatt tttgtcacaa cctctaattc aaggtgttca aaagccagat tctcagcatt
    45841 aagtcaaatt cacacaaaaa tgtggaaagg aactttcaaa aaaattttat atttattcta
    45901 tcaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa ctgaataaac
    45961 tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc ttcatagaaa
    46021 gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg tgtccggata
    46081 atcagtcatt taaacaatga ggttcaaaga tatgaaaagg ggaaatttga tgaagaaaat
    46141 aagcaatgaa atccaaattc tggcagaaac tttaactctt cccaaagtcg ttatagagca
    46201 aatgattgta caagcaaaaa acatccttga tgaggtgttt gagcaatata gtcatgaact
    46261 aatggatcct aataaagcaa aggcgactgc aaagaaaata gacaaagtct atgaagagga
    46321 accttttgca gcattagcta tttatctata tgcagcttct caaagttgga aaaaaattta
    46381 tcaaccattg aagattccaa gaactatcta tttggcaaca atgaatgctt ttactagatt
    46441 tattcatgaa catttccaag taacaggaag ctatcgcttt gaccgtggtt tttggatttg
    46501 gcattacact agtggcttga tctttcggat tggtgaattg gagtacgaaa tgacatattt
    46561 tccgaaaggg tacacggatc taagattaga aggaaagcag tgcctttctc ttcatattcc
    46621 ttccgatgca gatttatctc agaataagat aagagatagt tacgaaagtg ccattcgttt
    46681 ttttgaactt tattttcctg attaccatta ccaagtgatg tacacagata cttggttact
    46741 atcacccaat cttactaaat ggttgaaaaa agagtcaaaa atctgtcttt ttgcagccga
    46801 ttatcgatta ttatcagttg atgaacagga cgatagtgga gtcccttgga tctttggacg
    46861 agtagatgcc caaatacatg attatccaga aagtacgagt ttacagcgac aagcaaaaga
    46921 gcaattgctt gcaggcgagc acattggctc tggcttaggt attcttactt cataagttta
    46981 gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa agaacacttt tgtctcacgc
    47041 tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag ctgaaattca
    47101 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
    47161 gtcccagcct cttttttagt gttctatcga acacacagta cgttcaatag gtaaactagc
    47221 tataacaata gatattaaag ttaacggaca agagggcctt ttttaggtag ctaaaacgta
    47281 ataatgaaaa tttatttcca gagataggag tcaatcagct agtctgatcg actgttttaa
    47341 caagttgact ttcattttaa cttttgatac catttaaaac acaaatcgta attgtttcga
    47401 tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat atggaaaaaa
    47461 cagatatctc aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca cgaaaacgtg
    47521 cgctgaagat catcaaagat gtgaaacgga atagtgggaa aaggtaagaa gaaggaaact
    47581 taattaaaaa aatagccgaa aaacgttttt cggctatttt caataatcgg tttttgtgta
    47641 aggtttccga aagaactgct gttgattttc catttttaac atatgataaa ttggtagaat
    47701 ctttcttttc aaattgatac cattccctaa ttccagttat atagttaact acagaaccac
    47761 aaagcacgta cgtttataaa agtattatct gtagttattt ttcaattccc tttcaacctc
    47821 ttattttgac cagtagtatt gatcacttta ccttttaaca cctttgttcg tttttgaaac
    47881 atttttagtc aagaatggaa acgtaactac ataatcaaag gttttgcgaa atatttcatg
    47941 aaatatttca tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt ttaataagca
    48001 gttacgaaag ctcccaatat ctctaacgtt cagctcactc catttctttt cttcaaaaaa
    48061 atcagtgatt cgtatctctt tctatttgtc gaaaaagtta actttaaaaa gtaacgccga
    48121 aaaaatatgc aaaaaaataa aaaagcagtt actattgcta gaataacatc aaggaataat
    48181 aagttttggt tttcagataa tagtcgataa gttgtgtatt ggaattttag atacagtaat
    48241 aaagtatttt tcatgaaatt ttgtgagaat cactattaca agtatcttta ataaaattaa
    48301 tggttaagga ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt cgattgtgat
    48361 actagactca aagttgcagg aataagtttt tcaatttgtg attggaagat aaaaaatgaa
    48421 aaagaagggc ttttttttat actttgttga ttgtagtgtt tattttccct ctgtttagcc
    48481 aagtaacatt ttctatgact caaccagttt caagtgagga acatgataac ctttttcgtg
    48541 ctacagaaga gtttaagaaa tatcaggaaa atattgttcc ggagactcca ttagctagtg
    48601 agccaattac tgatattgat ggaaaccaaa taggatggta tatacagtac gaagttatgg
    48661 tggtaaataa tgactcaata gtaaaaaata cagtaggctt agagctaaca ccgattgaaa
    48721 aggtgagttc attgttaacg tatatttata ctgatgaaag tgacgagact gagatcattt
    48781 taacggatta ctcaaaaatt gctactgata aaaagtatat attgtggatg tagacactag
    48841 tgaaatactt tatgatatag aaattgatga aaatttacta gatgtagcac aagaaatcga
    48901 tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa aatatttatt
    48961 acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg gaaagaattc aagagtcgct
    49021 actcaattaa ctcgactccg acatttacag tttacagaga aggatcgata gaaaaaactg
    49081 ttttttggac taaagaaagt ggaatgtctt tagctgaagt agaagagttt ttggattatg
    49141 ttagtatgca acagtaaggg agttcttatt atgaaaaatt ttgacacata ttatgttatt
    49201 gttttttcga tatctttgct gttaatatta ctaactttaa tgcagtataa agagtatcca
    49261 gagtatttta ttttaacttt tgtatattta gtaagtagct acacacttct atataaattc
    49321 agaagttctt ctagagtttc taaagtttgt ttcttaatta gcttattgat tctataatat
    49381 ttttgattta tataggaaat taaaaatatt atagaatcaa cactcagaga acgggttggt
    49441 ttttaataga tttaataagt actgataatg aaatttctcc tggttgggaa aacgaagttg
    49501 gttaccctgt agaagaattt tttcaagatt actttgcgga gtatgccgat aggataatga
    49561 gagattacgt aacagataaa actcaagaag tcatcaatca atcccagtca ttaaatgaat
    49621 tatctgataa ggcgaaagaa gaagcagttg ccattatttc ttcagggatt agtgactata
    49681 ttgatatgag tggtgaaatc aattatggcg ataaagacaa aattgtacgc atgataaaag
    49741 cttttatcaa aacttttgat gtgaaggcag atcttaagtt taatgatact tacctagtag
    49801 gaaaattaat taatatcctt gggacagaga aagatacgga aacgtttatt tcctctttgt
    49861 ctatgttgtt tacagataag catttttcaa ctcagcttgg tggagaggaa ctagtggaaa
    49921 tcttttaacc attacgccca gcattaataa aagtatatca aaaaagtact cctgatgatt
    49981 tttaagattg gtttgagaaa taatgtgaaa acactatttt taatgttaat tatagataga
    50041 gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa gatacactgc tatatttcac
    50101 taaggatata gcagtgtatc tttcatgtcg tttcactggc agttttttat aaaaggaggt
    50161 gtgcatttca gttgcttttc tttggtagtt agttgaatcg tttaagaatt aacggatgtg
    50221 gcagacaggt gctctgtcca aaaaccagta aaatctttga tggttccatc tttatttagc
    50281 tgtgcctgtt caaatactgt tttgttttta ccgaatggat caattgcaat aattgttccg
    50341 atccccgcac gtttggcagc aagtaatcct gagtaagcat cttcgataac aagacaatca
    50401 gccggagcta agctcagttt gtctgccgct ttaagaaaaa tatccggttg cggtttccca
    50461 gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa accagcgggc taaatcgaaa
    50521 atatcaaaat aaaaagcaac attctctttg acagtagccg tggcaatcgt taatggaatc
    50581 tgttggtcag tcagctggtc aagagtcgct tcaagccctt ttgtcaactg aagctcgttt
    50641 tcattttgca agcaaagcat acgataatag ctttcttttt cttctgacaa tacggcgatc
    50701 tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca
    50761 tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt ttgtatgatc atgtatcata
    50821 tgcagccaag cagcttcatg cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat
    50881 ataatccctt tcatagttac gctcctatag tggtattttc ggttgaatca gcaatttgca
    50941 gatcgacata ttgtgcatag tgctcttgaa cctcccgcga gatctggcga tccgtaatga
    51001 gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc atctcgattg aattttttat
    51061 tatcaacgag tagatatttc ttacgggaat tatttagagc gatctgctgt gcttctcctt
    51121 cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt
    51181 ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat
    51241 ttttccggaa atcaccgcca atcagtagaa tttctggtcc atctgcttga gcaagaatat
    51301 caaaaactgg ataattattt gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca
    51361 atagttcgat tgttgtacct ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg
    51421 ctgctgtggc agcaatttct tttttttctt gaatctgcag agtggctttt tcagtgtgag
    51481 aaagttcttg atctaaagtg taggaaagac tttgagcccc tccatggaca cgcagcagct
    51541 tgcccgattt ttctaattca tctaaatctc tgcgaacggt catatcagaa acatctaatt
    51601 gattgatgat ttcattaacg gtaaggattc cttttttatt gaccatttct acgatagtaa
    51661 gcaatctttc ttttttcaac attgtcttca cctctaattt aaggataatc tatcatataa
    51721 acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg
    51781 ttacaataga ttttattgct gttttctggt ttttatgatt atttgtgttt gtttttgttg
    51841 acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa agagcgaaaa ctgattatag
    51901 atttgaggag agatgtacat gcttaaatta ccagaagatt ttatttttgg cggagccaca
    51961 gcagcctatc aggttgaagg agccactaag gaaggtggca aaggcgctgt tgcttgggat
    52021 gactttttag aggaacaagg gcgattcagt cccgatccgg caagtgattt ttatcatcaa
    52081 tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa atggtctaag attatccatt
    52141 gcttggagcc gcatttttcc tgatggagct gggaaaccaa atccagaagg aatcgctttt
    52201 tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat
    52261 cattttgata cccctaaacg tttgtttgac catggagatt ttttgaatcg agaaacaata
    52321 gaagcatttg tttcctatgc cattttttgt tttcatgaat tcaaagaagt caaggtgtgg
    52381 agtaccttta atgaaatcta tccagttgca accaatcaat atttgttagg tgttttccca
    52441 ccaggaatca aatatgattt tacaaagatc gtcgcctgtc ttcataatat gatggttgct
    52501 catgcccgtg tagtgaacta tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt
    52561 cactcattag agacgaaata tgcggcaaca gacgcgccgg aagataagca tgctgccttt
    52621 ttagacgatg cattgtcgat ccgtttctta ttagatgcta cctatttagg ctattattca
    52681 actgaaacct tgactgctct tgatgagata tgtgaagcaa atcaggccag ctatcatttt
    52741 cctgaagaag atttcgttga attgaaaaaa gcatccacgc gtaatgacta cttagggatc
    52801 aatcactacc aatgccattt tgtcaaagct tacgatggag aaaatgcaat ccatcataac
    52861 ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg gaatcggaga acgtatttat
    52921 aaagaaggaa ttcctcgaac tgattgggac tggctgattt atccagaagg tctatatgac
    52981 ttgttgctgc gaattaaaag cgattaccca cattacaaca aaatctatat cactgagaac
    53041 gggatggggt ataaagatca gtttgaagat ggcattatta tggatcagcc gagaatcgac
    53101 tatttgagag tctatttgga atccttaagc aaggcgataa cagccggggt aaatgtgaag
    53161 gggtattttt tgtggtcgct tatggatctt ttctcatgga caaatgggta taacaaaagg
    53221 tatggcttgt tttatgttga ttttgaaacc cagaagcgtt acccaaaaga atcagcttac
    53281 tggtacaagt tggtcagtga aaccaaaacg attatttaat aagtgataat ggaggaagaa
    53341 aagatgaata aagaggaatt acaaatgctt ggttttgaga tcgttgcata ttcaggcgac
    53401 gctcgcagta cattattgaa attattgaaa gaagtaagac aaggaaactt tgaacatgtt
    53461 gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg cacataattc gcaaacaaaa
    53521 attttagcgg aagaagcatc tgggaaagag atggagatgg gattcatttt tatccatggg
    53581 caggatcatt taatgaccac tttattgctg agagatctga ttcaagactt tattgtactg
    53641 tacagacaga atcaaggata ggagaagaac gctatggatg gaatcattaa acaaatcgaa
    53701 aaagggaaac cattttttga aaaaatctca cgtaatagtt atttaggagc cgttcgtgac
    53761 ggatttttaa cagcgatgcc agctatttta ttttcaagca ttttcatttt gattgctgcg
    53821 gttcctgaga tttttggtta ccaatggcct gaatctgtct ccacatggct atggaaagtc
    53881 tataattatt cgatgggtgt tgtagggtta ctcgtttcag caaccactgc tcgctgcttg
    53941 gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg
    54001 ctggcctctg tggtaagttt cctgttgtta agcgtctctc cgattgaagg tggattttca
    54061 acggaatata tggggacaaa gggactactg gcttcctttg tcgctgcatt tttaacagtt
    54121 aatttatata agttttgtgt gctgaaagat attaccatta aaatgcctaa agaagttcct
    54181 ggaacgattt cgcaaacctt ccgagatatt tttcctttct ctttttcagt atttgcggcc
    54241 gttttaattg atttaggcat tcgacaaatc ttccaaatct catttgcaga agcaatcatt
    54301 acggtgctgc agccgctatt tacagcagct gacgggtact taggaatcgc tattatctgg
    54361 ggagcgatgg cgctgttttg gtttgtcggt gttcatggac cgtcgatcgt ggagccggca
    54421 attgcggcaa ttatctatgc caatgtggaa accaatctgc aattgttcaa ttccggtgaa
    54481 caagcttcta aagtactgac ggtgggttta gggaattttg taggaactat gggaggaact
    54541 ggggcaacat tggttgttcc ttttatcttc ctactctttg ctaagtctaa gcagttgaag
    54601 gcagttggga aagcttcctt tattccagta agtttcgctg tcaacgaacc attgctattt
    54661 gcggcaccca tgattttgaa cccttatttt tttattccat ttttgttaac acctgttgta
    54721 aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca tgaacagctt tatgtacgtt
    54781 ttgccttggg caactccagc accgatcggt ttggttctag gaacgggcat gggtatctta
    54841 gcttttgttc tagcagcggt tctaatcgtc gttgacttcc tgatctatct accattttgc
    54901 aaagcatacg atgctgtctt agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc
    54961 gaggaaggtg cttctgccga tgtacgtgag gaagtacctg tggcagcgag tcaagttatc
    55021 agcgatgcaa ataaagaaat caatgtgttg gtactttgtg ctggcgcagg gacaagtgcg
    55081 atgttagcta atgctttaaa aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg
    55141 gcgggtgctt atggttctca ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca
    55201 ccgcaagtca attcttacta tgatgatatc aaagaagaca ccgatcgctt aggaatcaag
    55261 ctggttgcga caaaaggagc agaatatatc ggattaacga gagatcctga aaaagctgtt
    55321 cagtttattc tgaaccaatt tcaataaagg aggagccata atgaaaatgg gaatttggtt
    55381 gcgtaaagct gttttatttg cagctgtgtt aggggctttg ggggctgttt caaaaactgt
    55441 tgaagcatct gccagcgaat ttattcgcgg agcagatatt tcgatcctgt cagatatgga
    55501 aaaatctggt gcaaaatatt atgaagacga tatttcaaaa gatgccttag agattttaaa
    55561 aaataatggt gtcaactatg ttcgactgcg tttgtggcag gatccttata atagtaatgg
    55621 agaaagttat ggagcaggga caaatgatct ggaaacgacg attgaccttg caaaacgagc
    55681 aaaaaatctg ggacttaagg tgctgctgga ttttcactac agtgattttt gggttgatcc
    55741 tgggaaacag aatcttccta aagcatggca aggtctgacc tttgaagaaa tgaatacggc
    55801 attgtacgac tatacaaaga acgttctgtc ggaaatgaag cagctggacg tttatcctga
    55861 tatggttcag atcggcaatg aactgaatag cggaatgctc tggccttatg gcaagtcttg
    55921 gggagaaggc gggggagaat ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc
    55981 tgttcgagac actcaaccta aaacaactcc agtaatgctc cacttagctg atggtggcga
    56041 tacaggagca tttacttggt ggtttgatga aatcacgagt cgaggtgtgt catttgattt
    56101 aattggagtg tcgtattatc cgtattggca cggttcaatg aatgaacttc aaaccaatat
    56161 gaatgcgatc agccaacggt atggtaaaga agtgatcgta gttgaaacag cttatgggca
    56221 tacaacagcg aatgccgata ctatgccaaa tgcttttggt gaagccgaag cagctgcggg
    56281 gggatatgaa ccaacaccga caggacaagc agagtatttg ctggatcttg ccgatcggat
    56341 tcaggcagta ccgaataatc gcggggcggg cttcttctat tgggaacctc tttggtacaa
    56401 cggaaatgtg agttgggcaa cacaagcagg tatgaattat ctcagtgttc aatccactat
    56461 gggaaacgaa tgggacaacc aagcgatgtt tgattttgct ggaaacgcct tgccaagctt
    56521 gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag
    56581 cgatggacca acgaccagtc cttccaattg gcaaacctgg tttcaacaag gaaccgctaa
    56641 gggtacgatc aaaacggaag cgggcacttt ttttggggac tacaaactaa ctttttggca
    56701 ggatcaagca tacgaggcat cagcttatca aaatgttagt ggcataagca aaggaactta
    56761 cacattgagt gcctatgtaa tgtctggggg agagtttgat actaatcagt tatatatcaa
    56821 aaattatggt ggagcggaac taaatactgc ggttgttaaa acaagtgaat ggacaaaaat
    56881 tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa attggggttt atagcaaggc
    56941 ttcaggaaat gcttggacga acgttgattt tgtttcattg gtgaaacaat agactgcgga
    57001 tcgtttcgat tcaagatcgg aggttccctt cagccacatc gttagcgtag aaggcaggaa
    57061 tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac gatttccagt catttttacc
    57121 aaaacgagta aagcaccaga cttcatctac actttgtatt atgctaaaga gagttcaacg
    57181 atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc agataacata aagtttgttt
    57241 aagaaagggt aaccttagaa ttccgtcgtg aatatgattt gagcacttag atggatagaa
    57301 aaaagttctc ggaaaacagt tcgtttttcg agaacttctt tttatgataa gaatgaaaca
    57361 aattaactta tattatagtc tcttttggta gggaaccatc ttatttttaa tgaaaatcaa
    57421 gcattttagc tattgaagaa tgttgattta tagggattcc ccctccatat tatgttggct
    57481 ctttattccc actctgtttt caacgtaaag tgtataaaac agaatgcgat actatcaagg
    57541 gagttaaaac aatatacaga acagtaaaac actataaata aaaataaaag tgcaataaaa
    57601 gtgcaatttt tgcacctaat catactctgt ttatatatga aaactagatt ctgaatccaa
    57661 tatttggtta acgagaggcg ttcgaatttg aacgtctttt atatattact ttcaaataaa
    57721 tatgaggtag aatactatct attttctttt taacccaatt gttataatgg agagtgtaaa
    57781 atattttgtg taaatagaaa aaaggaagtc ccttctgtag aatagagtta ccacaacaca
    57841 ttcacagaag agaggacttc cctatgaacg attttactac agaaattcta aagactctag
    57901 cgaacaaagg cgatttgaat gaattattcc gtgtccattt ggagaaagca gtcaatacgc
    57961 ttctcaaaac ggagttaacg gctttcctag attacgaaaa gtatgatcgc attggtttta
    58021 acacgggtaa ttctcgtaac ggctcctatg accgtacggt caagaccgag tatggggaac
    58081 ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa gcaacagact gttcctgctt
    58141 atagacggac gaatgacacg ttagaggaga ccgtcattca cctcttccga aaaggtatta
    58201 ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg gcattactac acgccccaaa
    58261 ccatgtccaa tataacaaaa tcatttacag aagaggtaac ggcgtttaat gggcgggagc
    58321 ttcatgaccg ttatgctgct atttatatgg acgcaacgta tattccgtta aagcggaaaa
    58381 ccgtcgccaa ggaagctatt catatcgcag ttggcattcg cccggacgga tcaaaggaag
    58441 tattgagcta tgcgattgca ccgactgaat ccatcacgat ttgggaggaa attttattgg
    58501 accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat cacggatggc ttaaagggga
    58561 tggtaggagc gattagtcgg ttctatccca aagctcgttt tcaacattgt tgtgtacacg
    58621 tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg taaggaagtc tgtgatgatt
    58681 ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt ggaagcacgt ggtacttttg
    58741 cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc gattctttcg aacgatcact
    58801 tgctcacttt ctatgatttc cccttggcca tacgcaagag tatttattct acgaacttga
    58861 ttgaatcctt taataaacaa atcaagaaat acagccaccg caaggaacag ttccaaaatg
    58921 aagagtcgat ggaacgtttc ttagtctcgt cttttgatac ttacaaccaa aaattcctag
    58981 gtcgcagtca taaaggtttc caacaggccg aaggcgagct cgaacaaatg ctaagccaac
    59041 tgattgagaa ttagaaacaa tctgcagtag ggaagaatca tttacacaaa attattgacg
    59101 ctcccttata atgagaactt ttactaacat aatttatgat aaacttacta tataatcata
    59161 ttaattaaat agttaggaga tctgcttatg tttatatcta ctcttcaaat tcgaaatttt
    59221 agaaattatg aatccgaaat ctttcgattc gataatgaga caaatactat tataggtgag
    59281 aatgattcag ggaaaaccaa tgctcttact gcattgagaa tcttattaga tgatagttat
    59341 tactatagta gtaaaacact aaaagaatca gatttttttc atggaattca aaatgggtgg
    59401 caagggcatt ggattattat ctctgcgaca tttgaaggaa tcagcgaaga agaatttgac
    59461 aatgagattt gtgcttccat ttctttaaat tctgaaagtc aaacaatctt agaagaattg
    59521 atttcaaatg cagataaagg tgtaggtagt atttctctat ttattagacc taacaaagct
    59581 attagaaagc aattatttga tatttccact gaattagact ctcatcaatt taacgaattt
    59641 agaaattcta ttcgtctatc cgactatgaa ttttactaca catctaaatc aaatctaaat
    59701 ttctgtatca atgaaaatta tgataaattg gtaggaagac tcaatgacaa tatagctagt
    59761 aatcctgaag aagacgatga agctctgttg ggctcaagaa ttgacatgtc agatattttc
    59821 aagcatattt cggttgttta tgttgacgct cttcgagatg ttcttagaga aatgaaaaac
    59881 aatcgaaacc cagtcaaaag aatcatggaa acaattgaat caaaaatatc atcagataat
    59941 gttgagtcat taaaaacaat tatacagcaa ttaaatgaga ctattaccag tgttcctgaa
    60001 attaggaaaa ttggcgaaaa tattaatcgg caactaaata gcataattgg ttcagtgtat
    60061 tctcctaatc ttcttctttc ctcaactatg tcagatgata tgggttcttt agcaaaattt
    60121 ctttcaatga agccagaaca aaatatcgac ttagacttac tagggttagg acatctaaat
    60181 atgatttatt tagctttgaa aatagtcgaa tttgaagcgt gtcgttcaag agaactactt
    60241 aatattatgt tgattgaaga accagaagct catattcatc atcatattca aaaaactctg
    60301 ttcgaaggat taaatcttca aaaaaactat actcagattc tcatgaccac acattctgta
    60361 catttagctg aagtgtctga aatatctcgg atgaatgtat taaaatcgtt taatggcaaa
    60421 tctatcgtta tgaatccagc aaataatctg aactcatttg ctaaagaaaa gttaaaaaag
    60481 aacaatctaa atctaataaa cgcagtagag agatatttag atgttaaaag aaatggattg
    60541 cttttttcta aaggtgttat cttagttgaa ggagatgctg aagaaatact tattcctcaa
    60601 atagtaaaaa aattatttgg tataaactta gacgaacttg gaataggttt gattaatgtt
    60661 ggtagtactt cgtttgaata tatcgctagc ttgtttgacg atgatagaat acagagaaag
    60721 tgtgctattg taactgattt agatgaacag gcaatcccat cagaacatcg attgtataaa
    60781 agcactgcac aagagaaagg tcaagcaaga aaagaaaaat tatcctccct ttttaatgaa
    60841 aacaattggg ttaattgttt ttatgctaat acaacatttg aaattgagtt tttagatgtg
    60901 aataaagaaa atattctaac ttatgtcagt ccaattatag atgacgcatt caaagataaa
    60961 aaaacaataa ctgcttataa agaagaaata caaagtgaac aatatttaga aagaaattta
    61021 gcgatgttaa aactagccaa tcatgttggt aaggggtggt ttgcaattga gttaggtgaa
    61081 aaaattgact ttttaatcaa aattcctaag tatattctta atgctattgc ttttgcatgt
    61141 caggaaatag tagatctctc aatttataaa aaaattctac tccatacaat cgacttgtac
    61201 gaggaaacta cagaattgtc agctttatca gctagtttaa aaacaagtga tacagctgat
    61261 ttgaatatag caataatttc tttattagag tactttggta aagatgagga tgttgtgtat
    61321 ctaatcaatc agatagaaaa ttacacaaaa atactttatg ctgaggtttc aaaatgacaa
    61381 atatcttcga aaaaatcgta aatgatttaa atttagaaca aaaagatgtt gtggaaagtg
    61441 aagataacct ttatgtaaat gcatgtcctg ggagtgggaa aacaagggta cttacaagga
    61501 aaattgctta ccaagcaatt aaacacgatg gatcactaaa aagaataatt gctatcacat
    61561 acactaatag agctgctgaa gaaattaagg aacgcctaag tttacttagt atagatgatg
    61621 atattaatat ttgggtgggg acaatacatc agttttgtct tgaatttatt atttatcctt
    61681 ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat tattgatgat tatactcaaa
    61741 gagcttacgt cagccaaatt aataatatgc tgaatttaaa tctcaaaacg tatgaactga
    61801 acaaaattga tttgtcactt tctcatgatt ttaaaattaa tgaagttcag tatactcaag
    61861 tttctgaaat gtatcacact atgttggaaa aaaataaaga aattgatttt gatatgattt
    61921 tactagtagc gtataaaatt ttaactgtaa acagtaacgt tgcacgtaat attgctagta
    61981 atataagggc gatttatgtg gatgaatttc aagatactag agaattacaa tacaatgtaa
    62041 tagctaaatt acttcaaaac aaccctcaaa ttcagtcaat gtttgttgga gatattgacc
    62101 aagcaattta tggatcatta gatggtatag ctaaatcagt tgaagaacta gaaatgctaa
    62161 ctagtcacac ttttcaatca aagacgctac atggctgtta caggtctaat cagagattag
    62221 tagacttcta ctctaatttt cagagttgtc cttacgagat tgaaagcaga gggaataata
    62281 aaaacgaccg tggttttata agttatgatt gcaaagttac taaggaggat ttacccaaca
    62341 taataaaaaa tataataaag gaaaagattg aaagcggcat tcatgaaaaa gatatttgtg
    62401 tacttgctcc tcaacactat cctctgtttt cactgggtga gtatttaaag aaagaattac
    62461 cgtattgcaa ttttgatgct ataaatatat caccaattaa ggtaaataat catagtttat
    62521 ttttcaagtt agcaattttg tactttactg aatcaggtga aaaggttcga agaagaaagc
    62581 taattgcaaa cgatatttta attatattaa aaaatgaatt tggaatagaa gttaaagatt
    62641 attttattga cttagattta ttacagctaa taaacagtgt gaaaagaatg tatagtggag
    62701 acgacaacgg tgttgattta tacaaatttg ttaccaaaca tatatttgtt aatcttaata
    62761 ttgacgagaa aaattacccg tctattttta agcactatct attttttatt aacgaagtag
    62821 aagatcggat aaaaaataat tcattatcta gtagtgtatc tagttttaag aagatgttta
    62881 aatctcgaga tggtatcaca cttactacag cacataaagt taaaggaaat gagtatgata
    62941 ctgttatcgc tattaatctt ctaaacggga aaattcctca ttggaatgaa atttttaatg
    63001 tttctgataa aggaattagt tcagcgagaa aactactata cgtaatttgt tcaagagcaa
    63061 aaaataattt atttcttttc tcagaggtgg gttattttac gagtaaagga gacttgcttt
    63121 atcccactca attattagat aaaatagaat ttgattataa tatttaaaat acctaggtag
    63181 tcgttgtttt aaatatagta ggaatcggac gctcagtacg gagcgtctat ttttttaccc
    63241 aattttgaaa ggaagtgaac ttatgaaaat aaaaaatcaa gaatcaaaag gtcgctctcc
    63301 cccctttaaa accatcacac aaataagatt cattcaaaca taaaaacaga aaggataggt
    63361 aaaaatatgg aacttaaata tgtcattccc aatatggaaa aaacatttgg acaattagaa
    63421 tatgctggtg aagggaacat tgaacaacgt cgaataaatg gcagaccaac cattctctct
    63481 cgtagttaca atttatactc aacgattcaa cgagcagatg atattgttgt cattcttccc
    63541 tccgaagctg gcgagaaaca ttttgaatcc gacgaaaaag tcaaactcat caacccacat
    63601 attaccgcag aaggttataa aattggcaat cgaggattta ccaattacat tatgcacgca
    63661 gacgatatgg tgaaagcata agaaaggaaa ggtgaaaaaa tatgagatta gcagaaggta
    63721 ttgtgattga aaaagaccag acatttggca cattgaaatt ttccgccttg cgtcgtgagg
    63781 ttcgtatcca gaatgaagat ggtacggttt ccgaagaaat taaagaacgt acctatgact
    63841 taaaatcaaa aggacaagga cgcatgatac aagtcagtat ccccgcttct gttcctttga
    63901 aagaatttga ctacaacgca gaagtggaat taatcaatcc tgtggcagat accgtagcga
    63961 cggctacttt tcgaggggca gacgttgatt ggtacgtgaa agcagacgat cttgtattaa
    64021 aaggacagac aaaacctcat cagaaatata aaaaagaagc gtccgaaagt gaaaaatagg
    64081 tgttgcttat gacaaacaca ccattgattt tgaaagaaat tccgaaggat gaagccattt
    64141 cttttattcg gcaataccat tattcaaaga tactccctag attgtgtaaa tactttctag
    64201 ggattttttc agaagaaaaa ttattaggcg ttgtggaatt aggctggggt acgcaacccc
    64261 tacaaaccat acgcaaactc tttcccgaca gttcccttca aaccacagat tatttagaaa
    64321 ttggaaagat gtgttttctt ccagaaatga atcaaaccaa ttattttggc agtcaagctc
    64381 tttcagctct aattaagtgg ttgaaagaac atacggactg tcatttttta tatacactgg
    64441 cagatggaat tgagggaaaa tgtggctatg tctatcaagc gtccaacttc ttttactgcg
    64501 ggtatttcaa gacaagtgtc tatcgtgata agcagtcatg ggaaaagata caccctcgaa
    64561 gtgctcgcct tttattagag gaaaatgctc gctttgaaca ggtagagaaa aagcattggc
    64621 tttcccaggc attttgcgaa tacaaaggga ttgagaaaat caatggacgc atgtttcgct
    64681 atctctatcc gttgacaaaa gaagcgaaaa aactgttagg acacactctc tatcgacgtc
    64741 attattatcc gaaagaaaaa aatttacgct ttgaaaagcg aatcgcttat cagaaatatg
    64801 aagccatttc tcaaccaact tttgataaac aggctcgtat ctacaataca caattatttt
    64861 gataggtgga aaggaggatt tttatgccat tgcttcatga cagaggagag cgaatcaaag
    64921 ccaacgataa gcacttggtc tatcattttg tactaatttc attgctggta gtttttctag
    64981 tcgtcctact cccttttcat tggagagcgt tacaatcggt gaactggcaa gcagttcagt
    65041 tagatacatt tttctatcaa cttcatacgc catacttttg gagcagtatt ctcactgctc
    65101 tttttgtatg cggacttatc agctggcttt attatcacta tcgtatagat tctataaaac
    65161 agttagagca ccgtcaaaag ttagctcgta tgattttaga aaatcattgg tatgaagccg
    65221 aagaaactca atcagaggga ttctttaaag atttacccag caagaaaacc aaacaacgaa
    65281 tccgccattt tcctaaaata tattatcgaa tgaaagatgg actaatccat attcatgtag
    65341 aaatcacctt agggaaatat caagatcagc ttttgcacct agaaaacaaa ctagaaacag
    65401 gattgtattg tgaattagtg gaaaaaacgt tgaaagattc ttatgtagag tatgtcctac
    65461 tttatgatac tattggcaat cgaattggca ttgaagacgt ggtagctcaa gatggacgtg
    65521 tcaaattgat ggaatcggtc tattgggagt tcgattctct ccctcacatg ttaattgctg
    65581 ggggcactgg tagtggaaaa tcctatttta ttctcacatt gattgaatct ctcctgcata
    65641 caaatgcaaa gctttatatt cttgacccta aaaatgctga tttggcagac ttaggggcgg
    65701 tcatgaataa tgtctattac cgtaaagaag atatgttggc atgtatcaat caattttacg
    65761 aagatatgat tgatcgcagt gaaaccatga aacaacaccc taactataaa acaggagaaa
    65821 attatgctta tcttggtttg tctgccaatt ttcttatttt tgatgaatat gtggcaatga
    65881 tggatatgct aggtcgtgaa agtaccaccg ttattcataa actcaaacaa attgtaatgt
    65941 taggacgaca agcaggcttc tttttaattc ttgcctgtca acgtccagac gcaaaatatt
    66001 ttagtgatgg gattcgggac aactttaact tccgtgtggc tttgggtcgt atgtctgaaa
    66061 tgggctttgg tatgatgttc ggaagcgata cacaaaaaca atttttctta aaacccatca
    66121 agggaagagg ttacgtggat acaggaaaaa gtgtgattag tgagttttat acaccccttg
    66181 taccaaaaag gtacgatttc ttaggagaaa taggaaaagt cattcaaaaa aagcaatctg
    66241 agcctgtacc gcacgaagtg aaaggtacag gttcagactt gctttgattg gtgtggcgtt
    66301 agccacgcca atcctttagc cccctctatc taacaggggg gcacaaatac aactgaaaac
    66361 aggcaattct catcaaccaa tgttgatggt acaaaggatt gcggaaaata tgaaaggtgt
    66421 aaactcaagg gtcatagttt acatcttttt tagattggag gtttgcgaat ggaaaacgaa
    66481 aaaatttgga ttaaggaatt gaaagaaaag cgactggctt atggcgtttc tcaaaataaa
    66541 ctggcggttg cttctcatat taccagacct tacctatcgg atattgaaac aggcaaggca
    66601 gtccctactc aacaggtgaa agaagattta ctgaacgctt tggaacgctt caatcccgat
    66661 aacccattgg agatgttatt cgactatgta cggattcgat tccctaccaa tgatgtggct
    66721 caaattatcg gtgaggtttt acgtttgaat atggattata tgctacacga agactttggc
    66781 tattattctt atcccgaaca ctatcgtttc ggtgatattg ttgtcctagt ctctcatgat
    66841 gtaagtaaag gtgttttatt agaactgaaa ggaaaaggct gtcggcaatt tgaaaacttt
    66901 ctattggctc aacatcggag ctggtatgat ttctttcaag attgcatgga acataaaggt
    66961 attttcaaac gcttggattt agctatcaac gataaaacag ggattttgaa tattccagat
    67021 ttggcaaaga aatgtaaaca ggaagaatgc atttccgtct ttcgaagctt taaaaattat
    67081 cgcagtggtg aattggttca ccgtgatgaa aaaccataca tgggaaacac actttatatt
    67141 ggctcattaa aaagtgaagt ctatttctgt atatatgaga aagattatga gcaatacgtg
    67201 aaaaatgatg ttcccttaga agacgcagaa gtaaagaatc ggtttgaaat tcggttaaaa
    67261 aatgaccgag ccacccaagc catgaaagat ttattggcac atcaacaggc ggaaaaaaca
    67321 gcctttgaaa ttatcaatcg ctatatccgc tttgcagata aagacgacac taaacgtcgg
    67381 agtgattgga aaactaatga acgttgggaa tggtttatcg gtaaaaatcg tggagcatta
    67441 cgcctaacca cacagccaga gccttattct tttgaacgaa cattgaactg gttacatcat
    67501 caagtcgccc ctacattaaa aatcgcaagc attttagatg ttctcaacgg tacaaccatt
    67561 atctccacca tgattcagga agccaaactg acagaaaaac atgagaaatt aattgaacaa
    67621 caacatctag ccatggagga tttgattaca tgagcctttt aacaaaagaa ttaaagaagt
    67681 tagggtttca gtgtggcatt gagtttcaag cgtatataca aaatacaggg aaatatacgt
    67741 ctctcattat tgagggaaaa cggcaagctg gtgataccat ctatacctat gatttttaca
    67801 aggtgagatt ttataataat tataccaatc gagtaaccgt ctatggagag catttaaccc
    67861 cttttcaatt attaaggcgt gtaaaaagct atatctatta tcgagaaaaa tatcttaagg
    67921 aaaggaggac aatcacatga attttgggca gaatttgtac aactggttct tggataatgc
    67981 acagtcattg gtattgttag cgattgttgt cattggactg tttttaggtt ttaaacgaga
    68041 attttcaaaa ctcattggct tcttgattat tgctctaatc gccgtcggac ttgtgttcaa
    68101 tgcagctggc gtgaaagatg tgttattaaa cttatttaac cgaatcattg gtgcttaaca
    68161 gatatttttc atgataaaat aggaagtata tattttgtta tgaaaggatt tatgaatatg
    68221 tttacaaatg atcaatttaa atctctttta attaaaacat tagaaagaaa gagctcttca
    68281 aattattatg aaggaggtaa cgagattgtt tctaaaatga aaatatcaga ggtaacttta
    68341 gatgagactg acgattttac ttattataaa ggatataaaa agggctggaa tacgctttgc
    68401 acatatctta aaataagagt tccttttgat gatttagact tttttgaaag tcataaagat
    68461 ttgattaccc agacagcaag ctccatatat gataaacaag gtgataatgt attagtggat
    68521 actattttag taccacttcc agagaattac gaagtaatta actttagtca gttgaaaatt
    68581 agtgatgtgg tttcacaagc tattgaagac gctgagtctt ttatgagtaa tggtgaatat
    68641 caacgtgcct ttgacagagt tcacactgct tttcatggat atttaattga aatactaaaa
    68701 aaatatgaaa ttacagttcc tagagacgaa aatctatcta aattatatag taggatacaa
    68761 caattaattg aaaaagaaat tcaacctact gaacttgctg atatagtgaa gacaacgata
    68821 aggtcatcaa atggaatgat tagctctctt aatgaagcaa gaaataggca ttcacttgca
    68881 caccctaata caaatattat tggaaagaga gaagcaaaac taataatagg tatttcaagt
    68941 acagttacag attatattag cggatacctt gataagtagc gtcattaact gattgctact
    69001 ttttttatga ctatttttat aaaaaggatg tgataagaat tggaaatgaa agtctatgtg
    69061 gcaaatttag gacggtataa tgaaggtgaa ttagtcggag catggtttac tccaccaatt
    69121 gatgaggaag aaatggcaga aagaatcggt ttaaatgagg actacgaaga atatgcaata
    69181 catgattttg agcttccttt tgatgtggac gaatacacgc cgattagtga aatcaaccgt
    69241 ctatgtgaag ccattcaaga aatcgaaggt acacctattt ataatgaact caaagaaatt
    69301 caaggcatgt ggtttagtag cttggaagaa ttactggaaa acaaagagga tattcattgt
    69361 tatagtgatt gtgattcgat ggaagatgtc gcccgttatt acgtggaaga aacaggacag
    69421 cttggtgaag tcccttccaa tttacaaaat tatattgact accaatcttt gggacgtgat
    69481 atggaaatag agggtaatta cctagttacc tctcatgggg tatttgaata ttgtcagtaa
    69541 gtttaacaat ccgtgttata attaaattaa taaatagcac ggagaggtat aaattatggt
    69601 aaatgtattt gaaaatctta aactattaaa actagacatg aaagataaag gctgggtgat
    69661 agattccttt tactttcgtt acaaacagca aaattatatt gtacttgtta agctatttga
    69721 aaaagaggaa aaagttccag aatatgctct gttaaaacta gaatttttaa aggaaaacga
    69781 tttttcagat atgctagctg tctatgctaa ttcggtaaaa ttatatacag acacgaaaac
    69841 aataagagaa tattttggta tcgaatacag tagtaacttg ggagatgttt tatttcaatt
    69901 tagccaaact cttgctcgat ttattccgac agaagttagt gagaagaaaa atgaagacca
    69961 aaaagaagcc atgtgtttct cattaagtca aagtgattca gaagatccca gaaaaaaata
    70021 ttgtttttca gttagaagaa atcctttaaa gggaaatggt gagctgggaa aacgaagtcc
    70081 ttttaatgac aataaaacga gattgtatag acccagctta tatgaacgat tagggtcaga
    70141 taccaattta agtttcagat attcaatgaa tccagatgat gaagaaactg atgaaggtat
    70201 tattgcaaaa tggacaaaaa ataaaattga atagttaaag cattgaaaga gcaatcacct
    70261 attttgattg ttctttttct atttttacgg aaaggacgtg aagacatgaa aaaaataaga
    70321 agttacacca gtatttggtc agtagaaaaa gtgatttatg cgattaatga tttacaactc
    70381 cctttcccaa ttacctttac gcaaatgaca tggtttatcg tttctttgtt tgtcgtcata
    70441 ctctttggca gtttaccacc actttctctg attgacggag cactactcaa atacttaggg
    70501 attcccattg ttctcactgg gttcatgtgc caaaagacct ttgacggaaa gaaaccctat
    70561 ggatttctaa agtctatcat tacctatgct ctacgcccta aagtaaccta cgctggcaaa
    70621 caaatacgat taaaaaaaga aacaatgaat gaatcgatta cgattgtaag gagtgaacaa
    70681 ctagatggca tatccgatta aatatatgga agataatctc gtatttaatc atgatggcga
    70741 agtattcgct tattatgaat taattcccta taattacagt tttttaagtt ccgatgaaaa
    70801 aattcaagtt catgataatt tctgtcaatt gattgcccaa aatcgtgatg gcaagattca
    70861 tgctctgcaa ctagcaaccg aatcaagtat tcgagccaca caggaacaat cgaaaaaaga
    70921 aatcactgga cgattaaaag agatagctta cgaaaaaata gatgaacaga ccgaagcgtt
    70981 aatttctatg attggtgaca atcaagtaga ctatcgcttt tttattggtt ttaaactcta
    71041 cctcaatgaa caggaagtat ccgtaaaaaa tgttgggaaa gaaattcaaa aagcatttgc
    71101 ggaattcttt catggtgtga atcacaaatt aatgggcgat tttgtttcta tgcctatggc
    71161 ggaaatcgaa cgcttttcta agatggaaca gttgttagaa agtaaattat ccagacgctt
    71221 tcagattcgt aaattggaga aagatgattt tggttatatt attgaacatc tttacgggca
    71281 aacaggtgtc ccttttgatg actacgacta ccatttaccg aaacgatacg aagataaaaa
    71341 agtgttggta aagaaatacg atattctcaa acccactcgc tgtttaatcg aagaaaatca
    71401 acgctattta cggattgaac aggaagatac cactacttat gtggcgtatt ttacaattaa
    71461 tagtattgtt ggtgaattgg atttccctaa tagtgaaatt ttttattacc agcaacagca
    71521 gtttactttc cccgttgata ccagtatgaa tgttgaaatt gtcgccaata aaaaagcctt
    71581 atctaccgtt cgcaataaaa agaaagaact gaaagattta gatagccacg catgggaaag
    71641 cgacaacgaa accagtacta atgtggtgga cgctttagaa tctgtcgatg aattagaaag
    71701 taatttagac caaaccaaag aatctatgta caaactaagc tatgtggtac gggtatcagc
    71761 aaagaatttg gacgagctga aacaacgttg tgatgaagta aaagattttt atgatgatct
    71821 gtctatcaaa ttagtacgac cttttggcga tatgctaggc ttgcataatg aatttattcc
    71881 ctcaagcaag cgatatatca atgactatat tcaatatgtc acgtcggatt ttttggctgg
    71941 tcttggtttc ggtgctacac aaatgattgg tgaaaattct ggtatttacg tcggttacaa
    72001 tctcgataca ggcagaaata tttatctcca acctagcctt gcaagtcaag gaattaaagg
    72061 ctctgtaaca aatgcattag cgtccgcttt tattggttca ttaggtggcg gaaaatcatt
    72121 ctctaacaat atgctggtct attattcagt actctttggc ggacaggcaa ccattgttga
    72181 cccaaaagct gaacgtggtg aatggaaaga aaccttacct gatattgccc atgaaatcaa
    72241 tattgttaat ctcacgagtg agaacgacaa taaagggtta cttgaccctt atgtgatttt
    72301 aaaactcccg aaagattcag aaagccttgc cattgatatt ctcacgttct taacaggtat
    72361 ttccagccga gatagcgaga agtttcctgt cctgcgtaaa gctattcgga gtgtaactca
    72421 aagcaaacaa cgagggttgt tattggtgat tgatgaattg cgaaaagaag atacgcccat
    72481 cagcaatagt attgctgacc atatcgaaag ctttgtcgat tatgattttg cccatctgtt
    72541 attcagtgat ggcagtgtca cccaatccat tagcttggaa aaacaactca atattattca
    72601 agtggcagat ttggtcttac ccgattccaa tagctctttt gaagaataca ctaccatgga
    72661 attattaagt gtggcaatgc tgattgtcat tagtaccttt gctttagact ttatccactc
    72721 tgaccgttcc atttttaaaa ttgttgacct tgatgaggct tggagttttt tacaagtggc
    72781 acaaggaaaa gctctatcta tgcgattggt tcgagcagga cgtgccatga atgcaggggt
    72841 ctatttcgtt actcaaaacg cggacgattt actagatgaa aagatgaaga ataatattgg
    72901 cttgaaattt gcctttcgtt ccactgacct agtggaaatc aaaaagacct tagaattttt
    72961 tggtgtggac aaggaagatg aaaacaatca gaaacgctta cgtgaattag aaaatggaca
    73021 atgtttagtt tcagacatct atggtcgtgt aggggttatg cagtttcacc cgatttttga
    73081 agagttgctt catgcctttg ataccagacc gcctgtgcga aatgaggtgg aagaatgaca
    73141 caaaaacaaa agaaatttct tcactatagt tggataacgc tcctttgtgt gggcgttatt
    73201 ttacttttat taggcacgct gggcaatgca gtacaagcga caggcttagt tgatgaaacc
    73261 attgatacaa gcaatgaata ctccaagtat ggtttgaatc aatatcagct tgattattat
    73321 gtggacaata gctggggctg gcttccatgg aattggagtg atggcatagg acaatcggtg
    73381 atgtatggat tgtatgccat tactaatttt atttggacaa tttctcttta tctatcgaac
    73441 gccacaggtt atttggtgaa agaagcctat gccttagatt tcattagtca aacatcccaa
    73501 gcaattggcg aaaacatgca gacgattgca ggcattacac cgaatggctt ttccagttca
    73561 gggttttatg tcggtttcct ccttcttttt attctcattg ttggaatcta tgttgcctat
    73621 acaggattgg ttaagcgaga aacgacgaaa gtgatacgag ctgttttaaa ctttgtcgtg
    73681 gtgtttattc tatcggcttc ctttattgcg tatgcacccg attatattgc gaaaatcaat
    73741 gatttttcca aagatgtcag taccgccagt ctgaatattg ggacaaaaat tgttcttcct
    73801 aacacggata gtcaagggaa agacagtgtg gacatgatac gtgacagctt attctctgtt
    73861 caagtaaagc aaccatggct attgcttcaa tatggctcca ctgatgtaga agcgttaggg
    73921 gaagaacgtg tggaaagttt attgtctacc agtccagata caaacaatgg tgaagataga
    73981 gaaaatgtag tgattgaaga aattgaagat catgacaata cctatttaac cctaccgaag
    74041 acgatttcca gattaggaac agtcttcttt ttgtttgtat tcaatattgg tatctcgatt
    74101 tttgttttcc ttctaacagg aatcatgatt ttttcgcaag tgctatttat tattttcgct
    74161 atgtttttgc caatcagttt cctattaagt atgttgccga gttttgatgg catgggcagg
    74221 catgccatta ccaagttatt taatgtgatt atgacaaggg ctggtattac cttgattatt
    74281 accgttgcct ttagtatttc cactatgctt tattcgctgt cagcaagttc tccctttttc
    74341 atgattatgt ttttacaaat tgtgaccttt gcagggattt attttaaact gggcgattta
    74401 atgagcctat tcactttaca aagcaacgac tctcaaaaca tgggcagaca agtgtttcgc
    74461 agacctcgtc aaatgatgaa tcgacaaacc agaagatttc aacgcagtat gagtaagatt
    74521 tttcgtggaa aagcagggtc aacgaagcaa aagacaatga caacaaagca gaatcaaacc
    74581 aataaagcta accatactcg tcaaaatgaa cggactacac caaagaaaga aacagcactt
    74641 tccaatgtga aaaagcaaca aggcggtatc gggcaacgat taggtgaaaa gacagcccat
    74701 gtattggaca cgaaaaatcg tctagtcaat aaaagaaagc aggcaaaaca acagatacag
    74761 gcgacaccaa cccatgtcaa atatgccctt catcaaggaa aagagaatgt cactcataat
    74821 gtaacagact ttaaacaatc tatggcagat accaaagctc aacgcaaaca acagcgtatg
    74881 aaccagcagg agcaaaaacg aaagaacatt gctcaaaagc gtttggagct ggataaagca
    74941 aaagaaaaaa gacgaaaaga aaacccatca tctcctgttc agaataacca gcagcatgtt
    75001 cgcaagcgtc agcaaaccgt cacgcctgtt ttgtcacaaa acactaaacg tgaaaatcat
    75061 cagcgaccat tgaaaaaaga gcaaaaacaa aagacaccac cgaagcaatt caatcagagg
    75121 aagctaaata aaagaacgga taagcgagga caacaaacat gaagctaaaa ataacagtga
    75181 tttgtagtgt ggtcttctct ctcttctttt ttctcttatt attcctagtc attttctttg
    75241 cagatgatac ggacagtggc gaaaacaaca aggattcttc tattccacaa ggcggtgtga
    75301 ccgtttcacc agaagtgttg gcacaccgtc cgttgattga aaaatatggt aaggaatatg
    75361 ggattgaaga ctatgtttct tatatcttag ccattatgca agtggaatcg ggcggtacag
    75421 cagaagatgt tatgcaaagt tccgaatctt tgggtttacc gcccaacagt ttgagtaccg
    75481 aagagtccat caaacaaggg gttaaatatt tcagtgaatt attaaccagt gcagaacaac
    75541 aaggagtgga tatagattct gttatccaat cttataatta tggcggtggc tttttaaatt
    75601 atgtgaggag tcatgggaaa aaatatacct atgaattagc cgaacagttt tctaaagaga
    75661 agtcgggtgg tcaaaaagca gactatccca atcctatagc catacctgtg aatggtggtt
    75721 ggcgatataa ctatggaaat cagttttatg ttcagttggt ttcacaatac ctaaccgaca
    75781 cttccccaac agaatttgat gatgaaaccg tgcaagtcat tatggacgaa gccttgaaat
    75841 acgaagggtt tccctatgtc tttggagggg cgtcgcctac cacctctttt gattgtagtg
    75901 gcttaataca atgggtgtat gacaaagctg gcatttctct ccctcgtgtc gcccaagatc
    75961 agtatgacgc tacgcaagaa atctctatgg aagaagccca agcaggcgac cttatcttct
    76021 ttcattcaac ctataatgca ggcacatatg tgactcatgt ggcgatatat ttagagggaa
    76081 atcgctttta tcatgcaggc gacccaatcg gttatggcga cttaagcagt cgttattggc
    76141 aagaccatct gattggggct agacgtgtta ttcataacta agaaaggaat gaatcaaatg
    76201 attataaaaa tcgagcgaaa agaaaagaag gagaaaccaa agaaatctaa aaaaccgagc
    76261 aagcaaagaa aagttccgat gattaaagtt ggcacgcata agaaattaac ctttgtttta
    76321 tgggttttat tgattggtag tgtcggattt ggaatctata aaaattttac tgccattgac
    76381 actcatactg tccgtgaaac agaaatcatt aaacagcaga ttgtcgatac caaccaagta
    76441 gaaagttttg tgaaatcctt tgcgaaagac tacttttcgt ggcaacaatc ccaagaagcc
    76501 attgataaac gaaatgaaaa attgacccat tatttgacgg aagaattgca ggtattaaat
    76561 gaagaaatga ttcgtaagga tattcctact agttctagtg tgaacgatat acaggtgtgg
    76621 caggtttctc aagtgaatga gaacaccttt gaggtactgt tttctgttga acaagttatt
    76681 accgaagaca aagacaagga aactatatcg tctagctttc atgtggttgt ccatatagat
    76741 gaatcagata atatggttat tatcaaaaat ccgacaatga gtaagaaacc tcaaaaatcg
    76801 gactaccaac ccaaacagct agaaagtgac catacagtag atacagaaac gatggacgaa
    76861 atcactagct ttttagaaac gttcttccag ctctatccta ccgccacaga aaaagaattg
    76921 acttactatg taagtaatca tgtgttacct atgattaaca aggaatatgt tttcgaggaa
    76981 ttggtgaatc ctatctttac cagaaaagat aaccaagtaa tcgtgaatgt cgctgtgaaa
    77041 tatttagatc aagaaacaaa ggcaacccaa atctcgcaat ttgagttgat attagaaaaa
    77101 caagataatt ggaagattgt aaaataacaa atattggtac atgattacag atactttgta
    77161 atcatgtact ctttttgata aaaaattgga gattccttta caaatatgct cttacgtgct
    77221 attatttaag tgactattta aaaggagtta ataaatatgc ggcaaggtat tcttaaataa
    77281 actgtcaatt tgatagcggg aacaaataat tagatgtcct tttttaggag ggcttagttt
    77341 tttgtaccca gtttaagaat acctttatca tgtgattcta aagtatccag agaatatctg
    77401 tatgctttgt atacctatgg ttatgcataa aaatcccagt gataaaagta tttatcactg
    77461 ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat
    77521 tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa
    77581 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
    77641 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
    77701 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
    77761 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
    77821 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
    77881 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
    77941 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
    78001 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
    78061 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag agga
//
LOCUS       DAPBAB010000008        63275 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPBAB010000008 DAPBAB010000000
VERSION     DAPBAB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 63275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.008"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(92..490)
                     /locus_tag="RD444_000703"
     CDS             complement(92..490)
                     /locus_tag="RD444_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDT7548095.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(487..1260)
                     /locus_tag="RD444_000704"
     CDS             complement(487..1260)
                     /locus_tag="RD444_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDT7548096.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1257..1940)
                     /locus_tag="RD444_000705"
     CDS             complement(1257..1940)
                     /locus_tag="RD444_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDT7548097.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2107..2994)
                     /locus_tag="RD444_000706"
     CDS             complement(2107..2994)
                     /locus_tag="RD444_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7548098.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2991..3860)
                     /locus_tag="RD444_000707"
     CDS             complement(2991..3860)
                     /locus_tag="RD444_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7548099.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(3997..5283)
                     /locus_tag="RD444_000708"
     CDS             complement(3997..5283)
                     /locus_tag="RD444_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7548100.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5556..6134
                     /locus_tag="RD444_000709"
     CDS             5556..6134
                     /locus_tag="RD444_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7548101.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6134..7063
                     /locus_tag="RD444_000710"
     CDS             6134..7063
                     /locus_tag="RD444_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7548102.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7060..8796
                     /locus_tag="RD444_000711"
     CDS             7060..8796
                     /locus_tag="RD444_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDT7548103.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8859..9695)
                     /gene="pheA"
                     /locus_tag="RD444_000712"
     CDS             complement(8859..9695)
                     /gene="pheA"
                     /locus_tag="RD444_000712"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDT7548104.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9692..10201)
                     /locus_tag="RD444_000713"
     CDS             complement(9692..10201)
                     /locus_tag="RD444_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDT7548105.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10194..11489)
                     /gene="aroA"
                     /locus_tag="RD444_000714"
     CDS             complement(10194..11489)
                     /gene="aroA"
                     /locus_tag="RD444_000714"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDT7548106.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMSADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11502..12593)
                     /locus_tag="RD444_000715"
     CDS             complement(11502..12593)
                     /locus_tag="RD444_000715"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDT7548107.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12610..13776)
                     /gene="aroC"
                     /locus_tag="RD444_000716"
     CDS             complement(12610..13776)
                     /gene="aroC"
                     /locus_tag="RD444_000716"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDT7548108.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13778..14842)
                     /gene="aroB"
                     /locus_tag="RD444_000717"
     CDS             complement(13778..14842)
                     /gene="aroB"
                     /locus_tag="RD444_000717"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDT7548109.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14875..15897)
                     /gene="aroF"
                     /locus_tag="RD444_000718"
     CDS             complement(14875..15897)
                     /gene="aroF"
                     /locus_tag="RD444_000718"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDT7548110.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16556..17530)
                     /locus_tag="RD444_000719"
     CDS             complement(16556..17530)
                     /locus_tag="RD444_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDT7548111.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17511..19394)
                     /locus_tag="RD444_000720"
     CDS             complement(17511..19394)
                     /locus_tag="RD444_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDT7548112.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDIK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19412..21637)
                     /locus_tag="RD444_000721"
     CDS             complement(19412..21637)
                     /locus_tag="RD444_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDT7548113.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21752..22786
                     /locus_tag="RD444_000722"
     CDS             21752..22786
                     /locus_tag="RD444_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7548114.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22853..23866)
                     /locus_tag="RD444_000723"
     CDS             complement(22853..23866)
                     /locus_tag="RD444_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7548115.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23944..24132)
                     /locus_tag="RD444_000724"
     CDS             complement(23944..24132)
                     /locus_tag="RD444_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDT7548116.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24572..24700)
                     /locus_tag="RD444_000725"
     CDS             complement(24572..24700)
                     /locus_tag="RD444_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDT7548117.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24703..26076)
                     /gene="rlmD"
                     /locus_tag="RD444_000726"
     CDS             complement(24703..26076)
                     /gene="rlmD"
                     /locus_tag="RD444_000726"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDT7548118.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26187..27242)
                     /locus_tag="RD444_000727"
     CDS             complement(26187..27242)
                     /locus_tag="RD444_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDT7548119.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27252..28682)
                     /gene="gatB"
                     /locus_tag="RD444_000728"
     CDS             complement(27252..28682)
                     /gene="gatB"
                     /locus_tag="RD444_000728"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDT7548120.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28679..30151)
                     /gene="gatA"
                     /locus_tag="RD444_000729"
     CDS             complement(28679..30151)
                     /gene="gatA"
                     /locus_tag="RD444_000729"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDT7548121.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30151..30456)
                     /gene="gatC"
                     /locus_tag="RD444_000730"
     CDS             complement(30151..30456)
                     /gene="gatC"
                     /locus_tag="RD444_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDT7548122.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30562..32598)
                     /gene="ligA"
                     /locus_tag="RD444_000731"
     CDS             complement(30562..32598)
                     /gene="ligA"
                     /locus_tag="RD444_000731"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDT7548123.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32615..34849)
                     /gene="pcrA"
                     /locus_tag="RD444_000732"
     CDS             complement(32615..34849)
                     /gene="pcrA"
                     /locus_tag="RD444_000732"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDT7548124.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35004..36563
                     /locus_tag="RD444_000733"
     CDS             35004..36563
                     /locus_tag="RD444_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDT7548125.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36674..38605)
                     /locus_tag="RD444_000734"
     CDS             complement(36674..38605)
                     /locus_tag="RD444_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7548126.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38623..39537)
                     /gene="pfkB"
                     /locus_tag="RD444_000735"
     CDS             complement(38623..39537)
                     /gene="pfkB"
                     /locus_tag="RD444_000735"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDT7548127.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39534..40286)
                     /locus_tag="RD444_000736"
     CDS             complement(39534..40286)
                     /locus_tag="RD444_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7548128.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40446..40655)
                     /locus_tag="RD444_000737"
     CDS             complement(40446..40655)
                     /locus_tag="RD444_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548129.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40666..41994)
                     /locus_tag="RD444_000738"
     CDS             complement(40666..41994)
                     /locus_tag="RD444_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548130.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42130..42285
                     /locus_tag="RD444_000739"
     CDS             42130..42285
                     /locus_tag="RD444_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548131.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42359..42946)
                     /locus_tag="RD444_000740"
     CDS             complement(42359..42946)
                     /locus_tag="RD444_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDT7548132.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43029..44279)
                     /gene="clpX"
                     /locus_tag="RD444_000741"
     CDS             complement(43029..44279)
                     /gene="clpX"
                     /locus_tag="RD444_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDT7548133.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44526..45815)
                     /locus_tag="RD444_000742"
     CDS             complement(44526..45815)
                     /locus_tag="RD444_000742"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDT7548134.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(45969..46901)
                     /locus_tag="RD444_000743"
     CDS             complement(45969..46901)
                     /locus_tag="RD444_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548135.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47058..47717)
                     /locus_tag="RD444_000744"
     CDS             complement(47058..47717)
                     /locus_tag="RD444_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDT7548136.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47720..48745)
                     /gene="sppA"
                     /locus_tag="RD444_000745"
     CDS             complement(47720..48745)
                     /gene="sppA"
                     /locus_tag="RD444_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDT7548137.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48758..49048)
                     /locus_tag="RD444_000746"
     CDS             complement(48758..49048)
                     /locus_tag="RD444_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548138.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49457..51262
                     /gene="glmS"
                     /locus_tag="RD444_000747"
     CDS             49457..51262
                     /gene="glmS"
                     /locus_tag="RD444_000747"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDT7548139.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51334..52689)
                     /locus_tag="RD444_000748"
     CDS             complement(51334..52689)
                     /locus_tag="RD444_000748"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDT7548140.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52713..53882)
                     /locus_tag="RD444_000749"
     CDS             complement(52713..53882)
                     /locus_tag="RD444_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548141.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53879..54766)
                     /locus_tag="RD444_000750"
     CDS             complement(53879..54766)
                     /locus_tag="RD444_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDT7548142.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(54955..58647)
                     /gene="nifJ"
                     /locus_tag="RD444_000751"
     CDS             complement(54955..58647)
                     /gene="nifJ"
                     /locus_tag="RD444_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDT7548143.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58846..59367
                     /locus_tag="RD444_000752"
     CDS             58846..59367
                     /locus_tag="RD444_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDT7548144.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60066..60623)
                     /locus_tag="RD444_000753"
     CDS             complement(60066..60623)
                     /locus_tag="RD444_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548145.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60802..61413)
                     /locus_tag="RD444_000754"
     CDS             complement(60802..61413)
                     /locus_tag="RD444_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548146.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61526..61783)
                     /locus_tag="RD444_000755"
     CDS             complement(61526..61783)
                     /locus_tag="RD444_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548147.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62380..62658)
                     /locus_tag="RD444_000756"
     CDS             complement(62380..62658)
                     /locus_tag="RD444_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548148.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62692..62943)
                     /locus_tag="RD444_000757"
     CDS             complement(62692..62943)
                     /locus_tag="RD444_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548149.1"
                     /translation="MKEKPVNKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17609 a        13703 c        11200 g        20763 t
ORIGIN      
        1 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
       61 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      121 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      181 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      241 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      301 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      361 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      421 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      481 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      541 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      601 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      661 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      721 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      781 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      841 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      901 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
      961 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1021 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1081 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1141 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1201 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1261 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1321 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1381 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1441 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1501 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1561 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1621 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1681 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1741 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1801 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1861 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1921 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     1981 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2041 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2101 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2161 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2221 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2281 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2341 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2401 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2461 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2521 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2581 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2641 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2701 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2761 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2821 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2881 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     2941 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3001 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3061 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3121 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3181 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3241 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3301 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3361 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3421 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3481 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3541 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3601 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3661 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3721 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3781 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3841 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3901 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     3961 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4021 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4081 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4141 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4201 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4261 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4321 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4381 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4441 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4501 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4561 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4621 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4681 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4741 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4801 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4861 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4921 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     4981 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5041 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5101 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5161 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5221 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5281 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5341 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5401 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5461 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5521 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5581 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5641 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5701 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5761 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5821 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5881 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     5941 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6001 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6061 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6121 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6181 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6241 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6301 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6361 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6421 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6481 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6541 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6601 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6661 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6721 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6781 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6841 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6901 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     6961 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7021 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7081 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7141 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7201 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7261 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7321 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7381 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7441 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7501 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7561 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7621 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7681 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7741 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7801 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7861 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7921 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     7981 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8041 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8101 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8161 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8221 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8281 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8341 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8401 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8461 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8521 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8581 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8641 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8701 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8761 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8821 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8881 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     8941 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9001 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9061 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9121 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9181 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9241 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9301 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9361 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9421 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9481 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9541 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9601 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9661 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9721 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9781 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9841 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9901 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
     9961 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10021 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10081 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10141 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10201 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10261 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10321 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10381 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10441 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10501 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10561 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10621 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10681 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10741 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10801 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10861 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10921 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    10981 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11041 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gctcatctcg
    11101 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11161 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11221 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11281 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11341 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11401 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11461 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11521 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11581 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11641 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11701 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11761 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11821 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11881 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    11941 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12001 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12061 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12121 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12181 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12241 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12301 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12361 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12421 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12481 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12541 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12601 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12661 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12721 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12781 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12841 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12901 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    12961 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13021 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13081 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13141 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13201 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13261 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13321 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13381 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13441 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13501 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13561 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13621 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13681 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13741 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13801 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13861 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13921 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    13981 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14041 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14101 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14161 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14221 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14281 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14341 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14401 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14461 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14521 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14581 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14641 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14701 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14761 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14821 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14881 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    14941 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15001 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15061 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15121 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15181 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15241 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15301 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15361 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15421 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15481 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15541 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15601 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15661 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15721 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15781 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15841 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15901 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    15961 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16021 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16081 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16141 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16201 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16261 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16321 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16381 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16441 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16501 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16561 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16621 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16681 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16741 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16801 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16861 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16921 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    16981 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17041 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17101 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17161 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17221 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17281 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17341 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17401 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17461 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17521 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17581 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17641 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17701 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17761 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17821 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttatatct
    17881 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    17941 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18001 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18061 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18121 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18181 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18241 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18301 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18361 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18421 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18481 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18541 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18601 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18661 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18721 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18781 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18841 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18901 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    18961 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19021 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19081 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19141 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19201 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19261 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19321 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19381 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19441 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19501 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19561 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19621 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19681 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19741 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19801 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19861 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19921 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    19981 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20041 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20101 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20161 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20221 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20281 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20341 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20401 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20461 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20521 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20581 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20641 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20701 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20761 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20821 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20881 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    20941 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21001 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21061 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21121 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21181 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21241 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21301 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21361 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21421 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21481 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21541 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21601 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21661 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21721 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21781 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21841 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21901 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    21961 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22021 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22081 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22141 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22201 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22261 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22321 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22381 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22441 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22501 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22561 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22621 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22681 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22741 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22801 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22861 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22921 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    22981 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23041 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23101 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23161 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23221 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23281 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23341 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23401 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23461 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23521 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23581 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23641 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23701 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23761 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23821 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23881 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    23941 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24001 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24061 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24121 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24181 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24241 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24301 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24361 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24421 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24481 tattggcaaa tcgccaaaat aatgtagact aactaaaaag actaagaaac aataacaaaa
    24541 gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta gggccttttc
    24601 tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt
    24661 tctttttgat caattctttc attttttctt tttggtccat ttttattctc ctttaacgaa
    24721 tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct gaactttctt
    24781 cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg ccggattaca
    24841 tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa aatgatgatc
    24901 taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt tttcttgcca
    24961 ttttttcata actttttctg cttttccgac ttcataatga acattttcga ttttattatt
    25021 taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc catacacctt
    25081 atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag cgtcgatcac
    25141 aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat actctgcttg
    25201 ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc ctaatagctg
    25261 atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa tcacatttgt
    25321 tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt catgaatagc
    25381 ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa cgaccatcat
    25441 ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt catttttttc
    25501 ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt tgattgcttg
    25561 atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat ggctattctt
    25621 tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct gtgctttatt
    25681 acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt ctggcatctt
    25741 agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct gttgttcata
    25801 cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt ctacaggctg
    25861 acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact tattgcctgc
    25921 ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa ataatggata
    25981 accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt cgactgtata
    26041 tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta agcctatttt
    26101 tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc ggcactgacc
    26161 ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc gtctgctatt
    26221 tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac gaattctttg
    26281 ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg aatctgatcg
    26341 gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg tgcttccccg
    26401 ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc tgtttcaacg
    26461 aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg accgccattc
    26521 agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac aatgattaaa
    26581 gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc tccaacagag
    26641 ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc atcatacttc
    26701 aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc aagataagca
    26761 agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc tgtcaaatag
    26821 ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc catttttacg
    26881 gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg aatttttaat
    26941 gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg acggtgttca
    27001 agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc tgccactact
    27061 agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc tggttctggt
    27121 gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag gatatccgcc
    27181 aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg tgcttttttc
    27241 attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac gacaccagga
    27301 tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac cgcacgatct
    27361 tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc attaatgatt
    27421 ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac tacttcttgg
    27481 gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt tgagctgatc
    27541 gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa ttttgtctca
    27601 gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa ccagttagat
    27661 gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga catttctttt
    27721 gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat atagcgttcg
    27781 cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca ttcgtcatcg
    27841 atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga cccttctttg
    27901 acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt
    27961 tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa accttttttc
    28021 acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc ttcttgtcca
    28081 taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat tttcacatcg
    28141 ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta tgcttcttct
    28201 ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa
    28261 tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc ttctaaatgg
    28321 acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat ccatccgtca
    28381 tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc tgggtaaaag
    28441 taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc caaagctgct
    28501 ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg ataaccccag
    28561 tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc tggagagaaa
    28621 atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt ttcaaaattc
    28681 attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt gcttgttcaa
    28741 acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg atctgcaatc
    28801 caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt ccagccaagt
    28861 tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta atgttctcac
    28921 cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa tctgcaaaga
    28981 ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt
    29041 cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt acttcttcac
    29101 cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca ttttcagagc
    29161 gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa
    29221 tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct tccacaacag
    29281 cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg ccaggggcca
    29341 caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg tcgcctgtca
    29401 gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt tcgtcataac
    29461 cgctgatagc tgtcaatgct aatgcattat ctttcaccgt acgggtcatt ggtccgattt
    29521 ggtcaagaga tgaagcaaag gcaatcaaac caaaacgtga gacacggcca taagtaggtt
    29581 tcatccctac gatgccgtta aaagctgctg gttggcggat acttccccca gtatcactac
    29641 ctaaagaaac tggaatttgt ccagctgcaa ctgctgctgc agaaccacca gaagagccac
    29701 caggtacttt tgttgggtcc caagcatttt ttgttttctt gaaataagaa gtttctgtac
    29761 ttccacccat agcaaattca tccatgttta gcttaccaac agggatcata tctgcttgat
    29821 agattttatc catgaccgtt gcatcataaa ttggttcaaa attgtaaagg atcttagatg
    29881 cagcggttgt caagatatct tttgttacga tattatcctt gataccaatt ggaataccag
    29941 caaggacgtt gctttcatca atcccttttt catctaaagc ttttgcctgt tccaatgctt
    30001 tttcttcgtt caatgtaatg aatgcctcga cctcaggctc tgtttctttg attcgttcaa
    30061 atgttgtttt tgtcaaatct tgtacagtga tttctttcga tacaagcata tgatgcagtt
    30121 cttctagaga acgattgtgt aattctgtca ttatgcgcca gcctccccat tatcgatgat
    30181 tgcgggtact ttgataaagc cgtcttctgt ttccggcaca tttttcaata attcatcgcg
    30241 gttccatcca gcttgcgccc gatcttgtcg catcacgttt actgtttcaa ccacattcga
    30301 cgtaaatgga acaccttccg tatctacttc ttccagcatt tcaaccatat cgatgatttt
    30361 acctaattgg tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt
    30421 ggcaacgtgt ttcacttgtt cttcactaat cgccatacta ccccttcttt ctaaacgagt
    30481 taaaaacttt gctctctatt ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat
    30541 ttgtctgaaa aaactcattt ttcattcttc tactgagcga cttttagcca atgcatctgc
    30601 catttgatct tctgtccaaa cttcgattcc taattcttgt gctttggtca gcttgctgcc
    30661 agcatcttct cctgctacca cgatatccgt ttttttcgat acgctaccag tcactttccc
    30721 gccaagattt tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta gtttaccagt
    30781 aaggactact gttttttctt taaaaggaga ctcgacttct tgcaattggg catttcgaag
    30841 ccctttgtat tcgaaattga cgcctgcttg ttttaattca ttcatcaatt catggacttc
    30901 ttcgttactg aaataagtga ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc
    30961 aataatcgac tcatagtcac ttttactcaa tgtttctaga tcaccgaaat gttctgctaa
    31021 aatttttgct gctttcgctc cgacatgtcg gattcctaac ccgaaaatca accgctcgac
    31081 cgaattatct ttgctgttgt cgattgctgt caaaatatta tttgctgatt tttcttttat
    31141 tttgtccaac gtcaataact cttcttctgt caatttgtaa agatcagcaa catctgctac
    31201 tagtttttta tcatacatct gttctagtac gcgcggcccc aacccatcga tattcatcgc
    31261 atttcttgaa acaaagtggt tcaaaccttc tttcatttga gcagggcatt taggattgat
    31321 gcaccgtaag gctacttctt catccaaatg gacaagttca cttccacaaa ctggacaatg
    31381 tgtaggcaat tggtattctt cgctgtcttt aggtcgttta tctaacacga cttgcgaaac
    31441 ttctgggatg atgtctcccg ctttataaat cagaactgta tctttcaagc ggatatcctt
    31501 caacttaata taatcaccgt tatgcaggct ggcacggctt actgtcgttc ctgctactcg
    31561 gactggtgtc atgatagctg ttggcgttac tacccctgtt cgaccgactg tccattcgat
    31621 gttttcgatt agcgtttcaa cttcttctgg cggaaacttg taagctgctg cccatctcgg
    31681 tgctttgata gtaaaaccaa gttgatcttg tagcgagaat tcattcactt tgatgacgat
    31741 tccatcgatt tcatagggca aatccacacg tttgtcatga tattcttcaa tgtatgacca
    31801 cacttcatca atcgaatgac atagacgttt ttcccgattc gtgtggaatc caattttttc
    31861 tagctcttct agcgcatcat actgggtttt tgcttgcata ggaccgaaat cagctactgt
    31921 gtataaaaaa gtgtccagat ttcttttcgc tgtgatttta gaatctaact gacgcaaaga
    31981 accagctgcg gcgttccgag gattcgcaaa gatatctttt ccctctgctt ctctgtcttg
    32041 gttcaattgg acaaatgacc gttttggcat gaaacattcg cctcttactt cgatgttcat
    32101 tggttctttt aatttgattg ggaccgaccg gacagttttt aggttttcag tgatgttttc
    32161 tcctacagtt ccatccccgc gagtcgcacc tctcacgaaa tttccatctt cataacgcaa
    32221 agaaacggaa agtccatcta tttttagttc acagcaatag tcaactacac gtcccactgc
    32281 tttttgtaca cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt cgttcaaact
    32341 gtaaagcgga atatcatgag tgactttttc aaaaccttct aatacttttc cgcctactcg
    32401 ttgagtggga gaatcagacg tgatcaaatc aggatattgt tcctcgattg ctactaattc
    32461 tgcgtattct ttatcataaa catagtcttc cactgttggt ttatcttcta cgtagtattc
    32521 gcgagaccat tggttcagtc ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat
    32581 cgtcatttca tgttccatca tttaccctcc actactaagc tttttcaatt ggcgcaaagg
    32641 cagccaaaag acgttttact ccttgacttg ggaaagccac atctagttct agatccttcg
    32701 acgtcccgct tactcggacg actgttccta gaccccattt tttgtgtttg actttatctc
    32761 caggtttcca gctgttattt tcccctccag tagcactttt agagggaaca accgctgtct
    32821 ctggctgttt atagctatat gctgtttttt tagcgaatac aggcgttttt ttaggcgtag
    32881 gacgcatgcc ttcgatttcc agtaactcct gatcgatttc ttctacaaaa cgagaaggtc
    32941 ggttatattg cgtccgtcca tataatgttc tggagaaagc atttgttaaa tacaacgctt
    33001 cttctgctcg cgtgatcccc acatatgcga gacggcgttc ttcctccagt tcgctttctt
    33061 ccatcaatgc tcgagataaa ggaaaaaccc cctcttctag accaatcagg aatacaactg
    33121 gaaattctaa tcctttggct gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt
    33181 ctaaattgtc aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt agtttttctt
    33241 ctggtgcatc agcgtcttct tcattttgct gttcgaattg tttgtcgaat tcttgtgtga
    33301 ccgttaaaaa ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt
    33361 ttaagtcttc tagatatccg ctacgatcca aaacctcttt cgtcaattct gtcactgtca
    33421 agtatgggat catctgagtg acttcttgga tcatctctcc aaatgcacct aattgctgtc
    33481 ctgcttttcc accgatattt gccagatcca cattttgcgc tgcttcaagc agtggccatt
    33541 catgaagaga agcaaaactg cgtagttttt cgatagatcc tggtccgatg cctcgtttgg
    33601 gcgaattaac gatacgctca aaactgattg aatcttgagg atttaccagc acattcaaat
    33661 acgctaaaat atctttgatt tctttacgat cgtagaactt gtgccctcca accattttat
    33721 aaggaatatt tgctttcaac agagtttctt ccattacacg agattgtgcg ttcgtccggt
    33781 acaagatggc aaagtcgccg taattccgat gattgcttcg aatctcttct tgcatccggt
    33841 ctacgataaa acgggtttcg tctcgttcat tatctgcacg gtagtaagtg atcttgtttc
    33901 cttctttgtt ttctgtccac aagttttttg gttttcgatt gctattgttt tcaatgactt
    33961 ggtttgctgc gctaaggatg tttttcgtgg agcgatagtt ttgttctagc aaaattactg
    34021 cagcatctgg ataatctttt tcaaaatcca agatgttctg catatctgca ccgcgccagc
    34081 cgtaaatact ttgatctgca tctcccacga cgcacaggtt cctaaatcta cctgccaata
    34141 aattgactag cgtatactgt gcgtgatttg tatcttggta ctcatctaca tgaatataat
    34201 ggaatttgtt ttgataatac gtgagtgaat caggatgttc ttcaaataat cgaatcgtat
    34261 tcatgatcag gtcgtcaaag tccatacatt gattatattg aagctctttt tggtaagcag
    34321 cataacattt tgccgcaatc tcttcgtaat aactgccttg catttttgca aaatcctgag
    34381 gggtctgcag gctgtttttt gcttgagaga ttgttcctaa tatacttcga ggatcatatt
    34441 ttttcggatc aatgttcagt tcttttagta tccgtttcat caaggtcagc tgttcagaag
    34501 aatcgatgat tgtaaagttc cggttatacc cgataaaatc aacatctctt cttaaaatac
    34561 gcacacacat cgagtgaaaa gtcgataccc agacatcttc gcctccagat gcgagaatcg
    34621 cattgacacg ttctttcatt tcttttgcag ccttattggt aaaagtaatc gctaggatgt
    34681 tccaaggatt gacttctttt tcctcaatca aataggcaat gcggtgtgtc agcactctcg
    34741 tctttccact tcctgcacct gccattagca gtaatggtcc gtcagtgtga agaacagctt
    34801 ctttctgacg ggggttcatc ccgtttaata attcagcttt ggatgacatt aacaacatcc
    34861 tttcttttat caatcttgtt aattatagca ttttcttggc tttcattaaa ctagctagtt
    34921 caaaatcatt tgtttctttt tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa
    34981 ttaaaagaaa gcgaggcgtt tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga
    35041 ctcagatata ttttattcta aaaggaatat ttgttggact gctgacagga atcgtcgtta
    35101 gcctgtttcg attatctatc gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga
    35161 gtcgggaaca gccgatttac ttaattggcc tagctatttg ctgcatcctt gcagcatttt
    35221 tcgtgggtta tctggtcaaa aatgaaccgg atatcaaggg aagcgggatt ccacaagtag
    35281 agggacagct tcgcggggaa ttatcaatga actggttctc tgttttatgg aaaaagttca
    35341 ttggcggtgt actttcagtt ggtgcgggcc tttttctagg tagagaagga ccttctatac
    35401 agcttggcgc atccattgga caaggagctg gacaattgtt tcggagtcca tcttcagaag
    35461 aaaagatttt aatttctagc ggtgcaagta gcggacttgc agcagcattc aatgctccta
    35521 tcgcaggatt actctttgtt ttagaagaaa tccatcactc cttttctcca cttgtttggc
    35581 tgacatcatt tgcttcagca atcactgcta acttcgtctc tctttacttt ttcggcttga
    35641 gacctgtttt atatctaggt gaccttcctt ctttgccgtt gaaatattat ggttccttag
    35701 tgatcttagg agtacttcta ggcatcttag gttttattta tcagaatgtc ttgctggctt
    35761 tgccaaaatt ttacaaccga ttgcctttac cagcttattt tcacgggttc gttcctttct
    35821 tgctgatttt gccaattggt tatttatggc ctagtactct aggcggaggc aatcaaatcg
    35881 ttttagcttt tggacatgct tccttgccaa tcattatgat tatcggtttg tttgttcttc
    35941 gattcgtttt ttccatgatt tcttacggtg ggggtcttcc aggggggatt ttccttccga
    36001 tattgacatt gggggcttta atcggtagct tttatggaaa cattatggtt gatttcggga
    36061 tggatccaat ctacatcaag gattttgtcg ttattgccat ggctggttat ttcacagcga
    36121 ttggaaaagc accgctgaca gcaattattt tagtgacaga aatggttggc tctcttaatc
    36181 atctgatgcc tttaggattg gctgctcttg ttgcttatat cgtcaatgac cttctcggcg
    36241 gtaatccaat ttacgaatca ttattggaac gtctacttgg aaatactcag accgcctcac
    36301 taaaaggtgc aaggacaact tttgattttc cagtaacggc agagagttct ttagacggat
    36361 taatggtacg agacttcaat tggcctaaaa atatgctgct aatctctata cgcagaggaa
    36421 atcaggaaat cttgactcac ggtgatacga tcatgaatgt aggagatatt ttggttatct
    36481 tgactgatga agcaaacttg ccaagaatca agcaggaaat cgaggctaaa tcttctatca
    36541 caaatttgaa gcaatcattt taacgaacat ggaaaaacta ttttacaaca actattcatc
    36601 tacactactt gattctatat gattctataa aaaaagaaga ggctgggaaa acccagcctc
    36661 ttctttttta tttctatgct tctgctactg caggagcttt tctcaataaa ccataaatca
    36721 caccagatac gactgctccg atagcaataa acaccaaata catgaccggg tgactcagta
    36781 agaatacaac gaaaatcccg ccgtgaggag ccagtaattt gattccgaaa gctccgacta
    36841 atccgccagt taaagcagaa ccaataataa aacttggaat ggcacgaatc gggtcagctg
    36901 ctgcaaatgg aatcgatcct tccgtgacaa acgatagtcc catcacgatg ttcgtcaaac
    36961 cagcatcttg atctgttttg ctgaatttat tcttaaacaa acgtgtagca acgaaaattg
    37021 ccaatggagg taccattcct ccagccataa cagaagccat cacgacactt ccgccagttg
    37081 caacagtcgt agctaaagtt gctgtaccaa acacataagc ggctttgttg attggaccgc
    37141 ctaaatctgc cgccatcatt ccagctaata atgcaccaag taaagcagca ttcgaaccac
    37201 tcatacttgt caagaaaccg ttcaatgcat cattgattgc tttcataggg acattgacca
    37261 gcaacatcaa gaaaccagta atcagtaagc caaacactgg ataaaagagg atcgttttaa
    37321 ttccatccaa tgattttggt aagttcttga ataatttacg taaaaatacg atgacataac
    37381 cagctaagaa tccaccaatc agtgcaccta agaatcctgc accgcctgta ttagctaatg
    37441 caccagcagc aaatccaacg atcaaaccag ggcggtcacc gatacttgaa gcaataaatc
    37501 ctgctaatac cggcagcatg aaaccaaatg cagcttgtcc gatttggttg aaccagctag
    37561 cagcttgatt gtaattccct agatttgata attgatcatg tggcacgcca ataaattggt
    37621 cgatcatgaa agaaagcgca atagcgattc ctccaccaat aacgaaaggt aacatgtgtg
    37681 atacgccgtt catcaaatct ttgtaaattc tttgtccgat agtaccatcc gcatttcctt
    37741 cttctgaact gctttctttt ccatcaccat ggaatactgg tgcagtacca ctaagggcta
    37801 gattgatcaa ctcttctggt ttacggatac catcactgac aggacgattg atcagttctt
    37861 ttccgtcaaa gcgattcatc tctacttttt tgtctgctgc aacgattaca ccttctgcac
    37921 gggcgatgtc ttcagcagtc aatcggtttt tgattccttc tgaaccgttc gtttcgactt
    37981 tgatctccac acccatttct ttcgcttttt tcttcaaagc atcttctgcc atgtaagtgt
    38041 gagcgatccc tgttggacaa gctgtaacgg caacaacgaa ttttctttct ggatgagcag
    38101 aagcttctgc agcttgtgtt tcttgttctg ctttttcttc tgcttctttt tgctcttcag
    38161 ctgtttggaa caagtcttgg acttcttctg gtgtatttgc ttcttttaat tttcctacga
    38221 aatctgcatc aatcagcaat cgagatagtg ctgctaatgc ttgcaaatgt gtgtcgtttg
    38281 cgccttctgg agctgcgatc atgaaaaaca gataagttgg ctgtccgtct aaggcttcat
    38341 aatctactcc tttttggctt tttgcgaata aaactgttgc ttctttaact gcactgtttt
    38401 tcgcgtgagg catggcgatt ccatcaccaa gacctgtaga agtttgtgct tcgcgggcta
    38461 gaattccttc tttatatgtt tcgatatcgg aaatccggcc ggcatcaaac attttttgga
    38521 ccatttcatc gattgcgcct tttttatcag tcgcttgcaa atccatgatc atcgcttctt
    38581 tgaccattaa atccttgata ttcatgatta tcctccttga aactaatcaa tttttgtgat
    38641 ttcaacttct ggcagcaact catcaatcaa ttcgcgagct gccaagtcat cagaaaaagc
    38701 tgttgcgctt ccgcaagcaa ctccccattt aaaggcttcg attggatctt tcgtacggct
    38761 gtaagcccca ataaagccag caatcattga atcccctgca cctactgaat ttttcaatgg
    38821 gcgaactaat acattcgttt tataagttcc ttgttccgtg aagagcaaag caccgtctcc
    38881 agccattgag atcagcacat tctgtgcccc ttcagctagt aattttttcc catacggaat
    38941 gatttcttct acagattgga aggttgtgtt gtaaagctca gctaattcgt gattgtttgg
    39001 tttgatcaac aacggtttct taacaagtgc attttttagg tcatcacctg ttgtatcaat
    39061 cacgaaatct gcaccttttt ctcgaatgat atcaatcagt tcttgataaa atccttttcg
    39121 caggctagct ggcgcgcttc ctgacaatac gacgatatct ccttgtccta ctgttcctaa
    39181 tgcttgcttc aactcatcga tttcttcatc tgaaatagat gggcctaagc cattgatctc
    39241 agtttcagtg tctgatttta gtttgatatt gattcttgta tcttctttta cggttgtgaa
    39301 caaagactgg atttgttctt tttcaagcca cttcgaaata aattcgccag tgaaccctcc
    39361 taaaaaccct agtgctgtag attgagaatc gatccgtttc aaaatacggg aaacattgat
    39421 cccttttcct ccaggtaact tgaagtcttc cttcattcgg ttcaaatcgc ctagttttaa
    39481 cccatctacg cgtacgatat aatcaattga tggattaagt gttactgtgt aaatcattgt
    39541 attgcctcct taattgttgt cttttcacgg aattcattaa cgaattctaa tggacaacgc
    39601 tcggttatga tcgtggctgc ttccaaaggt gcaacagagg tgaaggacaa ctggttgaat
    39661 ttggtatgat caatcaatac aaatgtctct tcggaatttt tcatggctaa acgtttaaca
    39721 gctgcctctt ctggatccgg agtcgtgaat ccttgttcaa gatgggcacc gttcattccc
    39781 ataaatgctt tattaaaatg aaaatttttt agttgttcta tcgcattaga tcctaaaaca
    39841 gcattagttg ccagctttat cgttccacct aaaataatgg tagcaagctc caaatcaact
    39901 aaatttgccg catgttgaac ggaatttgtc actactgtga tctgttttcc ttgcagaaaa
    39961 ggaatcattt ctaacgtagt agagccagcg tctaaataaa tgacatcttc gtcattaatg
    40021 gtttcagctg ccgatttggc gatttgctgt ttttcgtgaa cgtttttgag tgatttttca
    40081 ctcatattct gctcaaaatt caataattgc tctttcttag ctccgccatg aactctttct
    40141 agaaacccac tattctccag ctcttgtaaa tcacgtctta tcgttgattc agaagcattc
    40201 aaaaggttta caagatcttg tgatttcacg attttctgct gttcaagcaa gtctaggatt
    40261 ttttgtctgc gttcctctgt aagcattttc acgatcacct caatacaaat aatagcatat
    40321 tttttttcta ctagcaatat aaatcagtca aaaacgttca taaaagttat aaatcagtca
    40381 aaaaaaaaat aaaaaaccag tcagaaagac tggtttttta tttttttgtc tatcatcaag
    40441 ccgttttatc gttttttatt ttcgcttctt gtttttttca tataaacaga agcaggtttt
    40501 ctttccttat gaagaagata ataataaagt cccatcccgc caaacatgac caaaaaaccg
    40561 atgactgatc gaataggaaa attaggaaca aaaaagagac agacggataa taatgatgcc
    40621 ggaaccatta ttcgataata gagttgttta tccattttct tcacctcata ttttttgtaa
    40681 agtaatcaat tgttctaatt ctcggtaatc taaaaattgc acacgcagat gctgctcacc
    40741 tttcttgaca tcgaattgga tacttcgccg ctttttcttt attcgtttga aggtaacatc
    40801 agagagtcta tgtttgattt cctttaacgg taattctgac agggattctg aaggaacata
    40861 gattcttaag gataaaagat actcttgata tttttcatct gttacctcta cttctagata
    40921 ttccctaata taataacaat aagcttcgat tccattatcc aaagcaacag tcggatcgat
    40981 aactttatag cacatcatgc gtgatcgatt ctcgtcaacc caaggaaaat acgcctgagc
    41041 atttagtgtt ccgcgaacac gcctttcttt ataatctcct tgttggatat cataactttt
    41101 tgttacaaaa aatgaagcat tctttttagg aacaggttgc atttgtgcat tctgatactg
    41161 gacagattgg atcgtatggt cagtgtaaat gtattcaacg aatggcggtt tcaataaacc
    41221 gacaccattg tcccaagcaa gatgatcaaa cccaagctgg tccctaaaat aggattgaaa
    41281 catttgattg tttggctttt gccagttaaa ggggtctcga taaagctgga tacatatgac
    41341 ttcgttttct aattccttca ctttttctac ttgttggatg tcgagaaaaa tcggtttagg
    41401 gatgatttga tgataatagc gactataata catttcccaa cacgaagtga aacataaacc
    41461 ttggtaaaaa agatcgtatc ctggaaattg gttgcaatcg actactactt ccccgttttg
    41521 ttgtttcatt ttaggtaaat gttcgatttc atcctttttc tcaaacaata gccgttgaga
    41581 gatctgtttt gtattgtgat agataaactc gctataagct cgcatatagg cgaacacccc
    41641 atgagcaatc atttttttct gaatgaagtc tcgaatcaaa aattgctttt ctttgaaaac
    41701 agagatagac aatacatttt ttatcaataa ttgattatct tccaagcgaa aactacattc
    41761 gttctcgtca tttcctacaa ctaaccaact ttcgtctctt accgatgcta gcattatttt
    41821 ttcttgaagc tttcgaagat catacggatc cgcttgttgt gtgaaagagc ggataaatgt
    41881 tggctggatc aacagacgtg tcaaaatagc ccaatcgctt atttcctgtt ttttttcttc
    41941 ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa tcattttctc
    42001 acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa actaaaactt
    42061 tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag aaacaaagag
    42121 gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact tttcgaatca
    42181 tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg atttctaaaa
    42241 ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa gctcaaaaaa
    42301 agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt tattcttttt
    42361 atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt tttgtaacgg
    42421 aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg atttcgctct
    42481 catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca acaattacag
    42541 ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa ggcttatgac
    42601 gcaaatctac taaagaaacg acagcacgta gctggtctct agtcgttaaa tatgtctcga
    42661 tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca tatcccggaa
    42721 catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg gtctttcctg
    42781 gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata aaagaagatt
    42841 tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct ggatattgtt
    42901 ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc tctctcctta
    42961 acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt tacggcagcc
    43021 tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg ctcaccagac
    43081 aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga agggatatca
    43141 aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc accggtattg
    43201 cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc aagttcggta
    43261 ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg ctcagtcaag
    43321 atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg taatcgtccg
    43381 ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg ctgcatgaca
    43441 ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt ttcgcccata
    43501 cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa caatatgttc
    43561 gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg aggtggcaca
    43621 cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc gccggaaacg
    43681 tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc atcaatatag
    43741 atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa tttaagcaaa
    43801 atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc atctgcgatt
    43861 gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt accagaccct
    43921 gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt gttttcttgt
    43981 gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga gcgttttgcg
    44041 cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg tttagggact
    44101 tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc tttacatagg
    44161 tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt cacttcttct
    44221 tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt gtcatacatg
    44281 gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt tacagtcttt
    44341 tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc aactgaccta
    44401 tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat cgtgtcacag
    44461 tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga tttattactt
    44521 gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attctattgc ttttttcatt
    44581 gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc gattggcatg
    44641 ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt tgcttgaaga
    44701 ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat ttctgcttca
    44761 ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata gtacatttca
    44821 ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg atgtacttca
    44881 tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc catacggatc
    44941 gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa ttctttgcga
    45001 tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt tgcgaattcg
    45061 tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt gaagactgct
    45121 tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt tacatctaga
    45181 ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa tgagcctgaa
    45241 cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc atctacaaag
    45301 ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc tttgataact
    45361 aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc atctgttact
    45421 tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac ttctggtttc
    45481 actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc aacatcgatt
    45541 tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata agcttctggt
    45601 aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg gttgaatact
    45661 tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt cactttatta
    45721 aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt taaaacacca
    45781 tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag tttagaaatt
    45841 cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa ccctttcaaa
    45901 ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat ctttttcact
    45961 gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt ttttaaaaaa
    46021 tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc ctctacagtc
    46081 agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata aacaattgca
    46141 gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc attcccgtaa
    46201 cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt tggcagatct
    46261 tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg atggcattcg
    46321 ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac ctctggcgat
    46381 acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc gatctcatca
    46441 atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt attgtggagt
    46501 gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg gattttttca
    46561 atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt tctagcatat
    46621 aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc ctcattttga
    46681 agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc gtccaactcg
    46741 taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc gtaagctcgt
    46801 tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg tgccactcgc
    46861 attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc tcctcttgct
    46921 catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt aaaaataact
    46981 gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt tcaactgtta
    47041 ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat cagattgatc
    47101 gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg attaaagact
    47161 gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca agcaccttct
    47221 ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac tcgaatacca
    47281 aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat aaaatacgct
    47341 agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga aaaattaagc
    47401 caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat acacaaaaga
    47461 tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt aggaaaatca
    47521 tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg ttgtttcttt
    47581 ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt ttgggaaaag
    47641 ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc cttattttca
    47701 tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt ggtgattccg
    47761 ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc ttcaatccct
    47821 ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc gtggaagaat
    47881 attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt aaagcatctt
    47941 caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca tcataaatcc
    48001 ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg ctgacgattg
    48061 ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct tcttcttttg
    48121 tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact gttgtatctt
    48181 ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc acaccaattg
    48241 agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa atatagtagc
    48301 caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt tctttacgaa
    48361 tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca ggagaattta
    48421 cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt tgttccataa
    48481 aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc gcagcaatcg
    48541 ttccatctac tgataatttt acgatttttt gcgtactgtc tcccttttcc aacacatttg
    48601 gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga tcgttttcct
    48661 gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca gcaatcagaa
    48721 ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt tttatgacga
    48781 tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc cgcgcatacc
    48841 cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct tgagcaactt
    48901 ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa aatattttcg
    48961 tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt aacattccca
    49021 gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat tactttctat
    49081 tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga cataaaaagg
    49141 acatttgaca aagaatttga tatataccaa tttatttttt attctttttt tgttgacaag
    49201 aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa aggaggatag
    49261 ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga gggtttatcg
    49321 aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa aaaatgtctg
    49381 caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat tcattataga
    49441 aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa atgtaacacc
    49501 aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag ctggtatctt
    49561 tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc aaaatttgaa
    49621 aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac gatgggcaac
    49681 tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg gacgcttcgt
    49741 gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt atttagcaaa
    49801 tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg aaactttcgc
    49861 gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca aaggttctta
    49921 cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta aaaataaaag
    49981 tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg caatggctat
    50041 gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta ccgtcacatc
    50101 agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt cattcgaagc
    50161 acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc tgaaagaaat
    50221 cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag aagacaatcg
    50281 tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa tctatatcgt
    50341 tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg aaaaattgac
    50401 acagattcct gttgaggttc atttagccag tgaatttggc tataacacac ctcttctttc
    50461 aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca gccgccaagt
    50521 gctggtaaaa atcaactgtc tcggctaccc ttcacttacg attacaaatg ttgctggttc
    50581 tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg aaatcgcagt
    50641 agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag ccaaagctat
    50701 cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg aattgagttt
    50761 agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag agttggcaga
    50821 agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg actacaacgt
    50881 ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag aaggttttgc
    50941 cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac cagtgatcgg
    51001 aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag aagtcgaaag
    51061 ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa gtgaccagct
    51121 ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac cgactcaatt
    51181 acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac gtaatttagc
    51241 gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat ctgacactgg
    51301 tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt cttctttgac
    51361 tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg cttctgccat
    51421 cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt ctgatcccat
    51481 ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg caccgttttt
    51541 atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg ctgcaagctc
    51601 tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg aaagcatccc
    51661 atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc cgccaaagtt
    51721 ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg taacgacatc
    51781 tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga cagtcgtaac
    51841 gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga taaacatgat
    51901 tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat cgccatcgcc
    51961 atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta aacgttctgg
    52021 atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc ccattgtata
    52081 aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg ctccgtttgc
    52141 tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta caaggaattg
    52201 agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt cagctgatgg
    52261 acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct cttggtcatc
    52321 tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg ctggattatg
    52381 agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat aagcaacacc
    52441 tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa gtcctgaaat
    52501 cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta acacgcgtgg
    52561 gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc ccagcttaaa
    52621 tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg ttccaaaata
    52681 tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt tgttctgctc
    52741 atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga gctgcttgtc
    52801 ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga atcagcagat
    52861 gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga agcagagcta
    52921 ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg cttgatactt
    52981 aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat acctgagaca
    53041 ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga gccgcttact
    53101 tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc tggtgttcct
    53161 tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc tctcactgtt
    53221 acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag cgcctgtatt
    53281 gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc agctactact
    53341 cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt aggactaacg
    53401 gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa cgtttccaca
    53461 tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg ttcgatcttt
    53521 gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt tccttcgccc
    53581 agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc ctctgcattc
    53641 aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata tccatggata
    53701 tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt ttgattatac
    53761 gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa gtacagcgca
    53821 atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc ttttgagatc
    53881 atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg tttttcttgt
    53941 tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat tcttgctggg
    54001 ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt tcttcagaga
    54061 caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg tgtcgagtcc
    54121 cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt gctactgcga
    54181 tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga ataaagatat
    54241 tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca atatactcat
    54301 ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg gacatatatt
    54361 ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg ctttttgatt
    54421 gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc ggctgaaaaa
    54481 tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa gacaaggtgt
    54541 gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca cctttcagaa
    54601 gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga taccagacaa
    54661 ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg gaaaacattt
    54721 gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa agcctctctt
    54781 ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc tgatatgctc
    54841 gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc gttttatttt
    54901 tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt tctgttattt
    54961 ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc tggcagctct
    55021 tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc tagtcttaac
    55081 gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta attctcccgt
    55141 cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt caaataaagc
    55201 ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt tcctcatgaa
    55261 atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt tcttgttttt
    55321 ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa cagcttcttt
    55381 tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac aaggcgtata
    55441 ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct tgatcgtttg
    55501 catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg tcatcgccat
    55561 cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag cgattgccga
    55621 agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt tatccaaaac
    55681 aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa aaccaatgtc
    55741 gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata gatcctttct
    55801 cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg cagcttgcag
    55861 ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc agtcttccat
    55921 caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac ttgccaaagc
    55981 ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat tatcttccaa
    56041 taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag gagaagcagg
    56101 agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca ttcgatcacc
    56161 gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc aagcgcccga
    56221 gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc ctttgactgg
    56281 atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt ctttctgttc
    56341 ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta cacacagacc
    56401 acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt ctgcgcctcg
    56461 catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat ctgctaaaaa
    56521 tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg tacagcgatc
    56581 actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag ccgttcctaa
    56641 aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga gttgatttcc
    56701 ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc ttttattgat
    56761 tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat gaagcagttc
    56821 ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt tatgtccata
    56881 gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa tgatgtcagc
    56941 taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac cgacttcttg
    57001 ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca agtaacgttt
    57061 taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat tcaataaaaa
    57121 aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag ctggtgtgtg
    57181 acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg tatctccaaa
    57241 acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa aatagccttg
    57301 cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg ctccaactgt
    57361 tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg tcaaatccag
    57421 actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta tggtaaaccg
    57481 ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga tttggtcggg
    57541 agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc gaagttcaga
    57601 atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc ctggttcttt
    57661 acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca cgaatgtttc
    57721 cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc cgtttgcatt
    57781 caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa caatgacttc
    57841 ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc gtaatcggtt
    57901 gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg ggttgatcgt
    57961 ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag tcggatgatt
    58021 gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca gaggagctaa
    58081 ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga atccatcaaa
    58141 gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag gtgctaaatc
    58201 catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc ttgcagccat
    58261 cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc gactagcaac
    58321 gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta tcatcaaaag
    58381 cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg atccatggac
    58441 cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt gaccgaatac
    58501 gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg aactgggtgt
    58561 tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg ctgccgtatt
    58621 gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt ctctcttatt
    58681 atctgttcca ttataacgta aaacatgcca tttttttctt ttatttataa aaatatttta
    58741 catgtatcta tttgaattgg tcatctaatt tttagacaaa cagtctaaat cgttgccttt
    58801 ttttgtcaga catgtataaa ctataagcga aagaggtgtt ttaacatgga tatatcagtg
    58861 attgatgcaa cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc gaatgctcct
    58921 aaaaaaatgg tcgaattcat caacttagct tgtccgtatt gccgacaatg gtttgaagaa
    58981 tcttatgagc tattagaaga agcagtccaa agcgggcaat tgcagcgagt gatcaaattg
    59041 ttcgataaag aaaaagaaag cttacagcga gggaacgtga tgcaccggta tctcacaatc
    59101 agcgatggcc aaaaagcgat caaagagatc aaacaaatat tcgatactca agacgagtgg
    59161 aaacatcttt ctctccaaga agtagcggat tttgcagtag ataaactaaa gctgacagaa
    59221 caaaaagacg aacagctgag ccaagctgtc atcaacgaag ctgagcaagc acatattcga
    59281 tttgttccaa ctgtcatctt gggtaaagaa atctttgatg aatctatttc aatagaggaa
    59341 ttaaaagaat tgatccaaac aaaataaaaa aataaggagc gtgggacaaa agtaaagaac
    59401 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    59461 ttcttggagt taaacgcttc tgtcccagcc tccttattcc gaataaatgg tgtccaaaag
    59521 cttgcatctg ccagattatc tcatttctta tgacaagcta agcaaattat ctccaccaaa
    59581 taatttattc atatttttga cttgcgcata ctaactaatt cttaaatttt catacaaaat
    59641 ttctcaattt cccttaacga gtaatattta tgtacggtga ttctcactag tatcccaatc
    59701 actttttaag ttatcccgat ttcaattcta cttcaaaaat agttgctttt atgtatgctg
    59761 ttagttgatt agatggtcat ttgttgagct aggtcgttta ttttaaacaa gttgtttctt
    59821 tagcttatac ctatctcgtc taccaaacgc aagatggcaa acacaaatgc atacgcatct
    59881 gtatttgcca ctttgattcg tataaagcta acaactatta actcctctca gctaatagtt
    59941 gctctctttt ttattcagat tcatttttgc tgatcacagc ctagtatttc aatggttttg
    60001 aacactaaac caaattgttc cattacaaca ttcactggct gatgaaatgg attagtagat
    60061 ttcgattatt caaagcgatt ttctattggt ggatcgtgtt cccaactaat aagacggatt
    60121 ccaaacctat cgtacagatc ttctaaacca cttataaatt ttgcacctat ttcaggtgtt
    60181 ttattgcgtg gatattctat tgttacgcca ttaaatggat accaattagg attaggctct
    60241 ctttttttct gtacatctcc catagaatag ttagcacttt ctgggacata atatattctt
    60301 cgaccatcct ctaattcatc taataagata tttcgtgatc gtctatcact tgctaaatgt
    60361 agacgccacg tgcgatacca tgccatgcta cctgcatctg cagaaaatcc atcaaataat
    60421 gcaacaattg tatcttctat cacaatcctt tccccatcta cttcatattc taatctaaaa
    60481 tcaaactccc ctcgggtaat ttctggtctt ggtggcttag gacccaatat tattctactt
    60541 aaaaccgcat tcgttcccag atgaaacaca gctataacaa caataatgat taatattttg
    60601 tttctatttg ataaatttct cattattcgc acatcacctc tacattctca catattagtt
    60661 cgtaatactc ctttaatatc aaaatatcta ctggtcgtaa taatattcgc tatgaattcc
    60721 tactactcgt ataagaaagg ccaaatcatc agcttttact tgctgatgat ttggcttgtt
    60781 tctatttatc gtaattgttt tctaatcacc aaaattgtta acaatcggtt cagatatttc
    60841 ccagttgatt aaggtaattc cgtattgtgt caaaacttct tgcgcttctc cgaaattaag
    60901 tacagttctt atgtatccac tttctcctaa tataaagtct acctctcttt taaaaccttc
    60961 ctcgagattt ctaagggcta tatgtggttt taatgacatt gattgtacat tttcccccat
    61021 ataatattgc gcacttcctc gactaaaata gattcctaga tcgtccccaa aaggtagatc
    61081 ttctctacca ctagctaatc tacttctcca tcttcgttct cttttcatag aacctgcgct
    61141 gaactcacac ccgttaaact cagctatgac agtatcttca attatgttaa tctgtccatc
    61201 tagttcgtat tcaatcctaa atggaaattc tccataggtg atttccggtt cgggtatttt
    61261 aggtgagatt aatgcaaaga aaggaaatat taagcttggc aacatcaaaa aaactcctcc
    61321 tatgatcaca attcccgatg taatccaaaa tataatttgt acaattctca ttgatccatt
    61381 gtattccatt ttattccccc tattactatt catatccgcc tatctatatc gatcttccaa
    61441 tgcagagata attgcttcca taccttgatt ttttactgag ttacctatgc gagatatcat
    61501 tctccactaa ggtgcatcat attgtctatc taacatgatt aattctccaa taggtgtata
    61561 ctcctccaaa aacaacattt aatgcatctc cctcgacaac ataagctacc atgtttgctg
    61621 tatatgtgtt tgagtctaat tcataagcag ataaatttac tgtcgctgga ttaaatataa
    61681 tcgcattcgt atttatagct actgaatttg ctgcttcagc accacctttt gaatgtccga
    61741 tcattgtgat ttcaatagta ggatttacgt ggacagccct caccgtttac cctgaccatt
    61801 atagaaatcg cttacttttt tgaactacgc aacaaaggat acaatgctcc taacttcact
    61861 gtctccattt ttgtccacta aggaatataa aaaataggca ttaattcatt taattcggtc
    61921 acaacacgtt accttttaaa tcattatact gtatctcata cattattatc aataaactat
    61981 tataaaaatg caaaaaatgc tttatcaatc tattttgtaa ttggtcataa tatcattgaa
    62041 atcataccct gtatcgattt ttcctatgaa tctcgtctat ttgtgattga cgaaaaagag
    62101 gcaagacaga cactggaatg gtaattttac ccactatttt gctccaacta tttgtaatcg
    62161 tacccacttt tgaaataccg agatgatcat tttaattgat atgctgattc ccaagcaaaa
    62221 ccccaagaaa ttaaatgctc tatcgcatct agcttcttgg gttttgctta tataaattcc
    62281 aacatttaaa ttttttatgc tgttcggtct cagaaatgct gtttttgctt atatcacaat
    62341 atcaagatgt caaattattc gtgttgccga tgactttgat taattttttt caaattgttt
    62401 attaagacta atgctgtctt tttgcagata ccaatgttcg aactttttaa cattatcata
    62461 tgatcctgca agttttaact tcttaccatt agatgttatc aaagttataa ttaaccttgc
    62521 ctttttccca ttaggatctt ttattttatg aattttctgc aggtaatctc tcctaatggc
    62581 agctattttt acctcatcag tgctgataaa ccattgatca tccacataca tactgcctgt
    62641 tatgttgtgt ttttccattt cttccgagaa cgaaaagttt aaggctcgtt cttacttcca
    62701 aagttgaatg agtccatgga ttggacttag tgtgagaatt gagaacaaca ataagaatcc
    62761 atatcctccc gcaaacatcc ctaaggctat actgtctctc catccataag taggattaga
    62821 agagaaaaga gtcaccgttt gtaataatat gaaaaggaat aatccagcac aaacaccaaa
    62881 gataacaact ccaaacattg aatttttata caatgaccat ttatttacag gcttttcttt
    62941 cattttactt ccccctactg ttctgttcac acggaatgaa ttcaaagttc ataagccctc
    63001 ctctttgaac atatgaatac gacttgctaa ctatacttta aaaaagcttg ttttaagcgg
    63061 ttataggcga gttgtaatct ctattcatct cattagcaaa taacttgccg aaaagtctac
    63121 tgaaattaaa gcagatagtg gaagaattat ttagccagga tagacactat ttgtgaaaca
    63181 catgtcttta cttttgaaat tctatgatga aaagccttgg aatacagtag ctcttaattg
    63241 gtaatacttt ttccattata aataacctct tttca
//
LOCUS       DAPBAB010000009        62167 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPBAB010000009 DAPBAB010000000
VERSION     DAPBAB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 62167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.009"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(148..2301)
                     /locus_tag="RD444_000758"
     CDS             complement(148..2301)
                     /locus_tag="RD444_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDT7548150.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="RD444_000759"
     CDS             complement(2595..3443)
                     /locus_tag="RD444_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7548151.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="RD444_000760"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="RD444_000760"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDT7548152.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="RD444_000761"
     CDS             complement(5944..6123)
                     /locus_tag="RD444_000761"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDT7548153.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="RD444_000762"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="RD444_000762"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDT7548154.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGVKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="RD444_000763"
     CDS             8271..10550
                     /locus_tag="RD444_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7548155.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="RD444_000764"
     CDS             complement(10643..11290)
                     /locus_tag="RD444_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548156.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="RD444_000765"
     CDS             complement(11469..12188)
                     /locus_tag="RD444_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7548157.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="RD444_000766"
     CDS             complement(12319..13470)
                     /locus_tag="RD444_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDT7548158.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="RD444_000767"
     CDS             complement(13687..14427)
                     /locus_tag="RD444_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7548159.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14787)
                     /gene="rsfS"
                     /locus_tag="RD444_000768"
     CDS             complement(14431..14787)
                     /gene="rsfS"
                     /locus_tag="RD444_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDT7548160.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="RD444_000769"
     CDS             complement(14811..15404)
                     /gene="yqeK"
                     /locus_tag="RD444_000769"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDT7548161.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16038)
                     /locus_tag="RD444_000770"
     CDS             complement(15388..16038)
                     /locus_tag="RD444_000770"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDT7548162.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="RD444_000771"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="RD444_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDT7548163.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="RD444_000772"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="RD444_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDT7548164.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="RD444_000773"
     CDS             complement(17481..18008)
                     /locus_tag="RD444_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDT7548165.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18235..19020)
                     /locus_tag="RD444_000774"
     CDS             complement(18235..19020)
                     /locus_tag="RD444_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDT7548166.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(19013..19882)
                     /locus_tag="RD444_000775"
     CDS             complement(19013..19882)
                     /locus_tag="RD444_000775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDT7548167.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHML
                     LELHTQKGWIDRG"
     gene            complement(19883..21262)
                     /locus_tag="RD444_000776"
     CDS             complement(19883..21262)
                     /locus_tag="RD444_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDT7548168.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPTRHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21270..21689)
                     /gene="fabZ"
                     /locus_tag="RD444_000777"
     CDS             complement(21270..21689)
                     /gene="fabZ"
                     /locus_tag="RD444_000777"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDT7548169.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21714..22190)
                     /gene="accB"
                     /locus_tag="RD444_000778"
     CDS             complement(21714..22190)
                     /gene="accB"
                     /locus_tag="RD444_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDT7548170.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22193..23428)
                     /gene="fabF"
                     /locus_tag="RD444_000779"
     CDS             complement(22193..23428)
                     /gene="fabF"
                     /locus_tag="RD444_000779"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDT7548171.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23447..24184)
                     /gene="fabG"
                     /locus_tag="RD444_000780"
     CDS             complement(23447..24184)
                     /gene="fabG"
                     /locus_tag="RD444_000780"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDT7548172.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24196..25113)
                     /gene="fabD"
                     /locus_tag="RD444_000781"
     CDS             complement(24196..25113)
                     /gene="fabD"
                     /locus_tag="RD444_000781"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDT7548173.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25220..25444)
                     /locus_tag="RD444_000782"
     CDS             complement(25220..25444)
                     /locus_tag="RD444_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDT7548174.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25497..26459)
                     /locus_tag="RD444_000783"
     CDS             complement(25497..26459)
                     /locus_tag="RD444_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDT7548175.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26469..26909)
                     /locus_tag="RD444_000784"
     CDS             complement(26469..26909)
                     /locus_tag="RD444_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7548176.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27215..28180
                     /locus_tag="RD444_000785"
     CDS             27215..28180
                     /locus_tag="RD444_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDT7548177.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(28237..28467)
                     /locus_tag="RD444_000786"
     CDS             complement(28237..28467)
                     /locus_tag="RD444_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDT7548178.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28553..29440)
                     /locus_tag="RD444_000787"
     CDS             complement(28553..29440)
                     /locus_tag="RD444_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDT7548179.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29529..30752)
                     /locus_tag="RD444_000788"
     CDS             complement(29529..30752)
                     /locus_tag="RD444_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7548180.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30769..31815)
                     /locus_tag="RD444_000789"
     CDS             complement(30769..31815)
                     /locus_tag="RD444_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7548181.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31979..33706)
                     /gene="ptsP"
                     /locus_tag="RD444_000790"
     CDS             complement(31979..33706)
                     /gene="ptsP"
                     /locus_tag="RD444_000790"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDT7548182.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33706..33972)
                     /locus_tag="RD444_000791"
     CDS             complement(33706..33972)
                     /locus_tag="RD444_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDT7548183.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34451..36685
                     /locus_tag="RD444_000792"
     CDS             34451..36685
                     /locus_tag="RD444_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7548184.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36833..37153
                     /locus_tag="RD444_000793"
     CDS             36833..37153
                     /locus_tag="RD444_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDT7548185.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37358..37582)
                     /locus_tag="RD444_000794"
     CDS             complement(37358..37582)
                     /locus_tag="RD444_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDT7548186.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37780..39360)
                     /locus_tag="RD444_000795"
     CDS             complement(37780..39360)
                     /locus_tag="RD444_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDT7548187.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39761..41137)
                     /locus_tag="RD444_000796"
     CDS             complement(39761..41137)
                     /locus_tag="RD444_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDT7548188.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41265..42383)
                     /locus_tag="RD444_000797"
     CDS             complement(41265..42383)
                     /locus_tag="RD444_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7548189.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42525..42805
                     /locus_tag="RD444_000798"
                     /pseudo
     CDS             42525..42805
                     /locus_tag="RD444_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(42883..43416)
                     /locus_tag="RD444_000799"
     CDS             complement(42883..43416)
                     /locus_tag="RD444_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDT7548190.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43510..44688)
                     /gene="mutY"
                     /locus_tag="RD444_000800"
     CDS             complement(43510..44688)
                     /gene="mutY"
                     /locus_tag="RD444_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDT7548191.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44681..45478)
                     /gene="recX"
                     /locus_tag="RD444_000801"
     CDS             complement(44681..45478)
                     /gene="recX"
                     /locus_tag="RD444_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDT7548192.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45585..46967
                     /gene="rlmD"
                     /locus_tag="RD444_000802"
     CDS             45585..46967
                     /gene="rlmD"
                     /locus_tag="RD444_000802"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDT7548193.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47141..47719)
                     /locus_tag="RD444_000803"
     CDS             complement(47141..47719)
                     /locus_tag="RD444_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDT7548194.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48041..48511
                     /locus_tag="RD444_000804"
     CDS             48041..48511
                     /locus_tag="RD444_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDT7548195.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48619..49845)
                     /locus_tag="RD444_000805"
     CDS             complement(48619..49845)
                     /locus_tag="RD444_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7548196.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50599..50799)
                     /locus_tag="RD444_000806"
     CDS             complement(50599..50799)
                     /locus_tag="RD444_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7548197.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(50933..51541)
                     /locus_tag="RD444_000807"
     CDS             complement(50933..51541)
                     /locus_tag="RD444_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDT7548198.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51626..52096)
                     /locus_tag="RD444_000808"
     CDS             complement(51626..52096)
                     /locus_tag="RD444_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDT7548199.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52144..53058)
                     /locus_tag="RD444_000809"
     CDS             complement(52144..53058)
                     /locus_tag="RD444_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7548200.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53204..54007
                     /locus_tag="RD444_000810"
     CDS             53204..54007
                     /locus_tag="RD444_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDT7548201.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54185..55132
                     /locus_tag="RD444_000811"
     CDS             54185..55132
                     /locus_tag="RD444_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDT7548202.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(55200..55493)
                     /locus_tag="RD444_000812"
     CDS             complement(55200..55493)
                     /locus_tag="RD444_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDT7548203.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(55521..56186)
                     /locus_tag="RD444_000813"
     CDS             complement(55521..56186)
                     /locus_tag="RD444_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDT7548204.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(56326..56958)
                     /locus_tag="RD444_000814"
     CDS             complement(56326..56958)
                     /locus_tag="RD444_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7548205.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(56965..58032)
                     /locus_tag="RD444_000815"
     CDS             complement(56965..58032)
                     /locus_tag="RD444_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7548206.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(58029..58760)
                     /locus_tag="RD444_000816"
     CDS             complement(58029..58760)
                     /locus_tag="RD444_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDT7548207.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(58928..59407)
                     /gene="greA"
                     /locus_tag="RD444_000817"
     CDS             complement(58928..59407)
                     /gene="greA"
                     /locus_tag="RD444_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDT7548208.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(59543..60718)
                     /gene="mltG"
                     /locus_tag="RD444_000818"
     CDS             complement(59543..60718)
                     /gene="mltG"
                     /locus_tag="RD444_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDT7548209.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            60883..62043
                     /locus_tag="RD444_000819"
     CDS             60883..62043
                     /locus_tag="RD444_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDT7548210.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT        17932 a        13119 c        10491 g        20625 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttggacgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgattggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tagctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gagcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ctccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactgatcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtaactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgt gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa atagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggtcgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaattg cctttacatc atagactctc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc agatggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaaacgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac attatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg cttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 aacatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaataagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc gttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt gtttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaatt tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aactgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta gccattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaactt cgttctggtg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaatgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caaccgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaattt ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactctggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccacagctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatctttct gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt taatgagaca tcttggatct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta agcgagcaag accattcttt actctttgct tagttgttaa acacctaata
    18241 ttggcggaac ctcttgtatc gtttttccag taattcatgc tggtcgaatc gaacaagttc
    18301 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    18361 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    18421 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    18481 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    18541 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    18601 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    18661 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaatg taatcaccgg
    18721 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    18781 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    18841 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    18901 aaacaaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    18961 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    19021 gatctatcca tcctttctgt gtatgcaatt caagaagcat atggattttc tgcttcaatt
    19081 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    19141 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    19201 tcaagctttg gggttctgct agaatgatat cgccttccat tgcaaaactg gcagttactc
    19261 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    19321 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    19381 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    19441 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    19501 tcactccaat aatgaaggga atcccttgaa tggttgcttt accagtgagg actgcttcat
    19561 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    19621 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    19681 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    19741 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    19801 caggaacaga tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt
    19861 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    19921 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcagggtgac
    19981 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    20041 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    20101 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctaccc
    20161 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    20221 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    20281 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    20341 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    20401 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    20461 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    20521 agatcactac agaaggagat tcttccagta ctttttgatt attacgttgc aaagaacaat
    20581 ctcgttctcc taaatgaatg acattgccaa aatgatcacc taaaatctga acttctatat
    20641 ggcgagtagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    20701 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    20761 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    20821 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    20881 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    20941 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    21001 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    21061 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    21121 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    21181 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    21241 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    21301 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    21361 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    21421 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    21481 gcaactgctc ctgcttgagc catcgcttca acaattaata cccctggcat aacaggttct
    21541 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    21601 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    21661 atgatttctt ttatttcttg tatgttcatt gatttccctc tttcctgctt tgcttatttt
    21721 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    21781 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    21841 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    21901 ggcgccggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    21961 actgccgatt ctttttcttt cattgtttct ggagcagttg ttgcttgttc aggtatgaca
    22021 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    22081 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    22141 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    22201 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    22261 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    22321 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    22381 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    22441 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    22501 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    22561 caccagcttc ttccatcgca agcttcattg ctttagacgc accagaacca tctggagtag
    22621 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aatgatttct ccatagattt
    22681 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacacct gctccttcac
    22741 ccatcacaaa accatttcga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    22801 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    22861 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    22921 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    22981 ctacgcgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    23041 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    23101 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    23161 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    23221 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    23281 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    23341 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    23401 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    23461 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    23521 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    23581 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    23641 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    23701 ctactacgcc agctttacta gctgcataat ttgcctgacc aatatttcca atcaacccag
    23761 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    23821 gctgcgtcat attgaaggtt ccaatcaaat tgatatcaag acattttttg aaatcttctg
    23881 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    23941 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    24001 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    24061 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    24121 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    24181 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    24241 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    24301 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    24361 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    24421 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    24481 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    24541 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    24601 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    24661 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    24721 cactgcaacc attttcccgc ttccagctgg ggcagcttct gtcatataag cgcctctttt
    24781 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    24841 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    24901 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    24961 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    25021 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    25081 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    25141 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    25201 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    25261 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    25321 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    25381 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    25441 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    25501 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    25561 tgctgttttc ccaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    25621 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    25681 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    25741 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    25801 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    25861 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    25921 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    25981 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    26041 tcaccaccaa tcactagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    26101 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    26161 cccaatttcc cttgtacttg tgctgctaca gatggcgtcg tgtaatcggg cgacattgtt
    26221 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    26281 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    26341 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    26401 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    26461 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    26521 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    26581 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    26641 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    26701 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    26761 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    26821 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    26881 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    26941 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    27001 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    27061 catttataat ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta
    27121 ttttctgtga acagaactat agaattcctt aaaacttgtc cattatttca aaatttgtta
    27181 tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct
    27241 ccccatgctg ccgggtgtct atcttatgaa agaccgtgat ggtaaggtca tctatgttgg
    27301 taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc
    27361 aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac
    27421 ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa
    27481 ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata agggatttgt
    27541 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
    27601 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
    27661 gttagctttg cgagcccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
    27721 ggaactccaa gggctgtttc aaggccaaaa taaaaagcta ctaacttact tgaaaaaaag
    27781 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
    27841 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcattttt taagaacacc
    27901 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
    27961 ttgttatggt caacttgcag aaacaatgat tgcctctgct gacgtttctc ctgacttcta
    28021 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
    28081 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ctgaaattga
    28141 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta
    28201 ggcatcttca acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc
    28261 catcgctacg atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa
    28321 ttcaaatgta tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc
    28381 aaaacggcga acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat
    28441 gattgcgact aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt
    28501 gtatcaaaag catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt
    28561 ttttttctgg tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat
    28621 tctcaaaacc tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca
    28681 atacaaggtc cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt
    28741 tgacaaaaaa accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag
    28801 accagttagc tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt
    28861 ttttttgtgc atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct
    28921 gattagccat ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac
    28981 caaattcttt gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga
    29041 tcgtcagtgt accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat
    29101 ctgcgccttt ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtacttgtag
    29161 tcaagtcaac taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt
    29221 agatttcttc tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg
    29281 ctaccgcttt tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg
    29341 cttttgtccg attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag
    29401 cacttcccat gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat
    29461 caaaaatcct tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg
    29521 tttgaatttt atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct
    29581 tcttcttttc ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc
    29641 atagcttcgc cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga
    29701 tctttaggaa cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt
    29761 tcaaaagttt tccctaatga aggatgatca aacaatcggt tcaaataagc atttccttcc
    29821 acgacacaag gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa
    29881 gtagaagcac tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc
    29941 acttctcctg tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac
    30001 tcttctacat aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg
    30061 acctgaggaa acccattaat caaggcttgg atattttttt cataagaaat cctgcttaaa
    30121 gaaagaatca tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc
    30181 tgctgagtga tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga
    30241 agcggtgcta ttacaccata gctacgtaat gtatcaataa cacgttcact tggacaaact
    30301 acacctgtcg tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc
    30361 acttttcctt taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc
    30421 agaggtattt tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta
    30481 tgggtatgaa taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct
    30541 ctaacaacaa ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca
    30601 tctttttcca acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca
    30661 tgtctttcta attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga
    30721 aagtaggtat cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac
    30781 ctgatttact ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt
    30841 tttaagttct tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg
    30901 tgtcgtactc ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt
    30961 tgtcttcccc atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc
    31021 tcgaacaagt acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc
    31081 ttcataagaa gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc
    31141 acgaatgtaa ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat
    31201 gttccgagga atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat
    31261 cttcttagca atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca
    31321 aatgattact ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat
    31381 tggatcaggg cgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg
    31441 gatactatag ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc
    31501 tttttggtac aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat
    31561 aaaggaattt cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc
    31621 tcttctggct ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat
    31681 gtcataatct tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt
    31741 ttgatgatca agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata
    31801 taataatacc ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact
    31861 gtcactcaaa cttaagccct atttttatct attcttactg acaaaaacgt tttcttaaaa
    31921 aagaacaaaa aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt
    31981 attttacata ttcatgaaca agttcaacga cttcttccat cgtatcgcat tctttcaatg
    32041 cacgatcagc aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt
    32101 taagaataga tgtcgcactc attgagaact catctagacc catacctact agcaatggaa
    32161 cagctgtttg atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg
    32221 ctgcatcaat tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt
    32281 aagaaacgcg ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac
    32341 ctacagagaa gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa
    32401 tctcgatcat gatgccgact tggatcgtat cagatacttc cttgccttca ctgatcaatt
    32461 tttgtttttc ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca
    32521 tagggaacat gatacgtaag ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg
    32581 tacggaacat ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga
    32641 atggattcat ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca
    32701 ttgtacgaac aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat
    32761 attggtcatc ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca
    32821 gaccaactgc ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt
    32881 ttgcagctaa ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt
    32941 cccattctgc tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt
    33001 catctgtagg atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt
    33061 ctttgacttt agcagtaatt tcttttgtac caacgattgc tggaatttct aaagaacgtg
    33121 ccatgatagc agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat
    33181 tgcgatctaa ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt
    33241 cgttgatcat tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag
    33301 caacatcacg aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga
    33361 acataccgat atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt
    33421 ctttaatatt ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg
    33481 catcaaatac ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct
    33541 gcaattcttc agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat
    33601 cttctactgt tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa
    33661 cggctacacc gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc
    33721 ttctttcttc attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc
    33781 agtaatagta acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt
    33841 caagtttact gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt
    33901 ttgtactaat agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc
    33961 tttcttttcc atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc
    34021 ttttttagta attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat
    34081 tttttttcgt tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc
    34141 cggattcttt ttcatccttt tcttcacatt ttataacaaa agtgtttatt ttctttttct
    34201 tgtcgctttc tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata
    34261 tgaaacgaat tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa
    34321 tggtcaatag agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt
    34381 cttttacctc atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg
    34441 tgaatgattc atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc
    34501 cactgtcaat ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa
    34561 aaatcaacaa aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag
    34621 tttggacgat cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca
    34681 aacaccacca acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg
    34741 caatacagaa ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg
    34801 tgatatcgat cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa
    34861 tcgccgaacc aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt
    34921 tgttgaaggt ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa
    34981 agaagtcatt cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt
    35041 tgaagaacgt atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt
    35101 catagatgaa gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc
    35161 tggtaatatt ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac
    35221 cttaaatgag taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt
    35281 acgggtagat gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg
    35341 ttatgaagac tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt
    35401 atccaatcgt tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga
    35461 aacaggttct aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa
    35521 actgcaagaa gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc
    35581 agcttattat cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga
    35641 agaggaaaca cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg
    35701 tattccagtg ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga
    35761 cttgaaagct catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg
    35821 tcgaaaccgt gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg
    35881 accaactggt gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc
    35941 acaagattca atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa
    36001 attgattggt tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa
    36061 agtacggaga aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga
    36121 tgttttgcat atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag
    36181 aacggtcagc tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt
    36241 agaagcaaac gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca
    36301 attaaacaac tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag
    36361 tgcattatca aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca
    36421 aatgctggca gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt
    36481 tgacttaggc tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca
    36541 aatcgaagat ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc
    36601 aaaactggat aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga
    36661 atcagccgaa gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga
    36721 gaaatgccgt ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga
    36781 aattataata agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt
    36841 ggtaaatgta acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc
    36901 ccatctagtc aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga
    36961 acataatgaa attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga
    37021 tgctaaaaat tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa
    37081 agtaatcgaa ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac
    37141 attagccgtg taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga
    37201 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    37261 aaataagcta aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct
    37321 tggagttaaa cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt
    37381 tcttttgtac ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt
    37441 cctctttatg gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt
    37501 ttacatctaa taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac
    37561 gtatttgcca ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa
    37621 ataattttta tatttttttg attagacatt tctatgattt taatatagta aaaagagccg
    37681 tcacggaaaa tccgtcacgg ctctaaacaa aatatgagag gctgtttttg tttggctatt
    37741 cttattactt gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt
    37801 tacattagga tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa
    37861 tggttggtcg aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa
    37921 atcttctgga tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc
    37981 gttatactca tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc
    38041 aatgatatat tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa
    38101 ttgatagatt cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat
    38161 aaacagctct ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa
    38221 caatgtatcc ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac
    38281 tgctttttcg acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag
    38341 ttttttattt gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat
    38401 tcggacaaaa gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac
    38461 gaaacctgaa aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc
    38521 ataaggagct ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt
    38581 caatgcagaa ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa
    38641 tgcatctcca gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt
    38701 ttcagttaga ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc
    38761 atcctgataa ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc
    38821 ttctagccct ttacccatac cgattggcca attcatcgga taagattcga tatctaatag
    38881 ctcttctaat tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat
    38941 aaaagtaaat atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc
    39001 ttcgatccct tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg
    39061 ataagtatct tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc
    39121 ttgataatca aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc
    39181 catccagtct gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg
    39241 gatagcccca ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg
    39301 ggaaatgatc gcaaaagttc tgcgattgtc cacttgttgc tttaattctg gattattcat
    39361 aacctaactc catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga
    39421 tctcttaaag aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt
    39481 ttccgagtaa acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa
    39541 tacgaaaagc tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca
    39601 acctttgtca actactacaa ctgaatcagt atagcatgga ttcatcgaat gaaaaagcca
    39661 ccaaaccata agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt
    39721 gtttattcaa gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat
    39781 cttcgaattc ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg
    39841 gatcatgaaa aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca
    39901 gcgtaatatt atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg
    39961 ctgtaatcaa ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag
    40021 cttcattgaa ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt
    40081 tttcataaag attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta
    40141 aaaggtcttc tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca
    40201 tctgattctg cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa
    40261 cgaataaagg ctcctgcatg attttcttga tatctaaatt atcaaaaccg aatttatggg
    40321 cagcttttag taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat
    40381 aaacagggat tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt
    40441 cgttgatatc aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg
    40501 tatcaagaga aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt
    40561 tttcaagcat ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca
    40621 tcggcgtgat acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag
    40681 ctactacacc aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat
    40741 ttaaagcaat acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag
    40801 ccagcacaat gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta
    40861 accgttccga taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc
    40921 ctacttggat tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag
    40981 atttcacatc tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag
    41041 caatttcgga agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct
    41101 gcgctaacag cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa
    41161 ttaattttta gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca
    41221 caaagcaatt ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca
    41281 attttcttct tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg
    41341 atcgtcttgc agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata
    41401 tttccagcta ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca
    41461 attacattag gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa
    41521 caaagtaatg cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct
    41581 agataaggtc ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa
    41641 gcataatcca caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat
    41701 tcgattgctt gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc
    41761 acaatttggt ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct
    41821 ccgtctttca acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa
    41881 gcgatcgttc caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg
    41941 attgtcccgt aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac
    42001 aaaggcaatt cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata
    42061 ttagatgcca atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa
    42121 ataattgctg caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt
    42181 gctttcatca acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt
    42241 acaggtgcaa ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct
    42301 gatgaaatca tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta
    42361 gactgtctaa gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat
    42421 attcgttaaa aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt
    42481 gttttatttt ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa
    42541 caaaagatac aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg
    42601 tagtcgaaca gggtatgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc
    42661 acttcgtcct gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag
    42721 aaaacggaaa aatgaaatta cagcggatgg cgatcctttt gaagatgctg gcatgcctca
    42781 tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct
    42841 tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg
    42901 cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt
    42961 gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat cgttcggata
    43021 attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc
    43081 aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa
    43141 aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca ttgctattac
    43201 attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg
    43261 tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt
    43321 ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg
    43381 gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt
    43441 ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat
    43501 gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt
    43561 catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt
    43621 tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac
    43681 atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac
    43741 aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa
    43801 cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt
    43861 cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc
    43921 aaaataatac gctccagaat gattttgaag agccgcactg atataataaa cgtcttttgg
    43981 ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca
    44041 aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc
    44101 tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat
    44161 cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa
    44221 tctgctgact actcgcatca cgttgccgtc aaccgcgggt tctggaagtc caaatgcgat
    44281 acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg
    44341 cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt
    44401 ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc
    44461 atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac
    44521 tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg
    44581 attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg
    44641 aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct
    44701 tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct
    44761 ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc
    44821 cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcca
    44881 tcgcttgttg gatcacttct tgagaaaaac ctttcgtcat caatgtttgt tggatttttc
    44941 tcaataattc tttttggctt tttccgcgat tttttccaaa cgctttttcg gctgtctgat
    45001 gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag
    45061 gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc
    45121 gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt
    45181 ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg
    45241 ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac
    45301 cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc
    45361 cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag
    45421 ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa
    45481 acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt
    45541 atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg
    45601 tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa
    45661 tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac
    45721 tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa
    45781 aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc
    45841 aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag
    45901 cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga
    45961 tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga
    46021 ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag
    46081 actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga
    46141 ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca
    46201 tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa
    46261 aattcccgca aaaaaacgca atgatctgtg agatcaatga acgactgccc gaagttgttt
    46321 caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc
    46381 acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc
    46441 ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca
    46501 aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga
    46561 tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag
    46621 caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag
    46681 tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta
    46741 tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat
    46801 accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg
    46861 ttaatttaag taaaaagttc aagattgaat atttacagtc tgtcgacatg tttcctcaga
    46921 ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg
    46981 tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt
    47041 tttatgaata ccgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa
    47101 agggttgtga catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta
    47161 tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat
    47221 atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg
    47281 ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat
    47341 tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat
    47401 tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct
    47461 gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg
    47521 tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta
    47581 agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat
    47641 gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata
    47701 acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt
    47761 tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa
    47821 aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga
    47881 accaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta
    47941 ataacaacaa tcatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag
    48001 ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga taaaagatat
    48061 tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag
    48121 cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac cacctagctg
    48181 gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt
    48241 acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt
    48301 catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc
    48361 aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctaca
    48421 tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct
    48481 attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa
    48541 cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat
    48601 taaccaaact atttttttct agtacccact tgttgttcca tactcattat tctggtcata
    48661 accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg
    48721 tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg
    48781 atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc
    48841 accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt
    48901 tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg
    48961 gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc
    49021 ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt tcgtcgctac
    49081 atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat
    49141 ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac
    49201 ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga
    49261 ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc
    49321 caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc
    49381 catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat
    49441 tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt tatctaccgt
    49501 tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt
    49561 ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc
    49621 taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct
    49681 tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt
    49741 ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac
    49801 aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt
    49861 cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca
    49921 taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa tttctcaaaa
    49981 ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca
    50041 atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag gtattttcca
    50101 aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct
    50161 accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt ccttttgtta
    50221 aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac
    50281 tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg
    50341 attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca
    50401 aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag
    50461 aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta
    50521 gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg
    50581 tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc
    50641 ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg
    50701 aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc
    50761 tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc ctccaaaaaa
    50821 gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt
    50881 cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg
    50941 ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat
    51001 aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta
    51061 gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta
    51121 attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt
    51181 ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta
    51241 tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt
    51301 tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca
    51361 tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg
    51421 catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt
    51481 cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca
    51541 taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga gtaacaaaga
    51601 gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt
    51661 caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc gctaggataa
    51721 caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca
    51781 ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc
    51841 attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg
    51901 ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag gccatactta
    51961 aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac
    52021 caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact
    52081 gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat catactctat
    52141 atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt
    52201 ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg
    52261 aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa
    52321 aataattatc ttttcgctat actgccaaat atggacatga tgaacattct ttaccccatc
    52381 gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac tttgtatcaa
    52441 aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat
    52501 cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat
    52561 aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt
    52621 caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc
    52681 aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt
    52741 gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc
    52801 actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc
    52861 cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc
    52921 atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat
    52981 caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg
    53041 cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt
    53101 ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca cttgatactt
    53161 cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt
    53221 aattatcagt tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg
    53281 gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca
    53341 agtttttcaa gtcttaaata acattcaatt ggatgggcaa aagattgcta ctcgcattcc
    53401 tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca
    53461 aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc
    53521 tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc
    53581 tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta
    53641 caccaatgaa tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc
    53701 atctatacca ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct
    53761 gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg
    53821 actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc
    53881 tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat
    53941 tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat
    54001 caattaagaa ataaagacaa ctcgttattt ctacttaaat agaccctttt agaggttgtg
    54061 acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc
    54121 aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt
    54181 aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga
    54241 aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca
    54301 aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc
    54361 taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt
    54421 ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga
    54481 gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca
    54541 gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat
    54601 actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga
    54661 catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt
    54721 ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc
    54781 tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa
    54841 atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc
    54901 tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc
    54961 acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt
    55021 gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt
    55081 tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa
    55141 aacaatattt tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt
    55201 tattgttttt taaagtgata ggttggctcc tccaggttgg gatcataatc aacaagtaaa
    55261 tcatatcctt taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatccatt
    55321 tccactttct ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca
    55381 tgaacctctg ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga
    55441 tcaatttctg agtcaaacca tttttgtgct tttggcgtta ccgttaattt catcccaaca
    55501 acctactttc tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct
    55561 gtctctccaa taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa
    55621 gcgataaccg tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg
    55681 aaatttaatt cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct
    55741 aaagaatatt tatctgacag ctctaagtag tctagaatat tctttgatac taagttatgt
    55801 gcaatccgta tccccatgtc tcgctcaggg tgtacgacac gatctgctcc gatcttctct
    55861 agtacacggg catggtactc attttgggct tttgctaaaa tattcggtac gcccatttcc
    55921 tttaccatca gagtaactaa gatgctggct tggatatctt ctccaatagc gacaattaca
    55981 tgatcgaaat ttcttatacc taatgaacga agtgtcatct catcctgtgc atttgccaca
    56041 acagcatggg tggctatatt catatattca ttgactcgat cttcattact gtcaatagcc
    56101 agtacttctt gtccagcttc gatcagtgta cggcagatgc ttccgccaaa tcgtccaagg
    56161 cctacaattg caaaattttg tttcaccatt ttcctccttt agtatcttct tttctcagta
    56221 taacaaagcc tttccaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat
    56281 aatagttatt ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc
    56341 atgtttaaac gcatagatag ctgcttgagt tcggtcttct acctctaact tagctaatat
    56401 attcgacaca tgcgttttga ctgttttcag tgtgataaac aattcatccg cgatttcctg
    56461 attacttttc ccttcagaaa tcagcatcaa aatttcattt tctcgattcg ttaattcttc
    56521 atgcaatact ggatcatttc gccgactcat tttttccatc atttttgttg tcacttcagg
    56581 ttctagtact cgctcgccgc gttgcgtagc ccttatagca tcagctattt catgtgctgt
    56641 agacgtcttc aacaaatatc ctgctgctcc tgcttcaatt gcaggataaa ctttttcatc
    56701 atcaataaaa cttgtgacga taatgatttt ggcttttggc caatctttta agattgcttt
    56761 tgttgattcg atgccatcca tttcttccat gactagatcc attaaaatga catccggtcg
    56821 aagtgccatc gccttttcat agccttgacg tccattttca gcttctccaa tcacttcaat
    56881 gtcttcttgg atagataaat aagaagagac gcctaagcgt accatttcat ggtcatctac
    56941 taataaaact tttatcatct ttatctatgc ctccttcatc aaagggacct tggtctccac
    57001 gctcgtacct tgccctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg
    57061 ttctttgata ttgttcaatc cataacttcc cgttttggtt tcattcatat taaatcctgt
    57121 cccgtcgtca atgatacgta ataaaagatt gttatctatt ttatgcaaat agacttctaa
    57181 ttcattagcc ttagcatgtc ttaatgtgtt tgataacaat tcttgaacga ttcggaacaa
    57241 gtgatcctca atggaactag ttaattttac atcttctacc tcccatttta gcgaaatctg
    57301 gattttattc tgcaattcct tcaataactg ctcaatgcct tgttttaaac ttttttcttc
    57361 taaattgact ggacgcagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat
    57421 gatctctgcc accattttca actgcttacg gaacatttct ggtgtctcac ttttttctgc
    57481 ttgctccgtc aatgccgaca tcatcatcat cgctgcaaat aattgctgtg aaactgaatc
    57541 atgaagctca cgagctaatc gatgcctttc taattttaaa atctcttctt tggtttgtcc
    57601 atctacatat tgaggacggc tagtcataat ctgtaattca ctggatactt cgatcatttt
    57661 ttcttttatt ttagtaattt ccatatcaat atctttgatg tacagatcat ccgacgcgct
    57721 tgggatcggc tgatgcaaaa gtttggattc ataattgcca gaagaaagtg cacgaagctt
    57781 ttcttcgatt ttcccgtatt ttgccttttg aataagtgat aacaatagaa atacgatcaa
    57841 tcctacacct aatgaaattg ccatcaaatg gaagataagc ggaacgtata ataaacgcgc
    57901 acgcagcaat tctccccacc aatgactttg gttactggca taaaaataag tgaaaagtga
    57961 aaatagaata ataaggaaag ccgcaatccc cgaataaaca gctagcattg ctttggatat
    58021 tcttcccatc atattcgtat cacctcgaca tctccaacca aagtattggt aatgatcttc
    58081 aatctgcgag gattttcatc ataatcactg ctgcagattt tgatctgttc gttttttaag
    58141 gaaaactgct cttcttcaaa caagacattt cctactaatg tcgcatgctc taaacgaata
    58201 gccactccca ggggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata
    58261 ttatcatctt ttggcaaaag tgtattgccc aaatcgatga tagtatcacc agaaatgatg
    58321 gcaatgttga tatcatccca ttcatagacg tcgtttccaa tacgttggtt accaaataat
    58381 tgctgtcttt tacgctcatt attatgtgtt tttaattgct ctgtttggac catcatgatc
    58441 tgtttttttc gccaaaaagc attttttgtc aaatctattc ctgaaatctc tacacctttc
    58501 agcccgataa ataaaacggc caatacaacc atcatccata aagcaggact gttgactaag
    58561 ctaaaaaaaa tgaccaagct ccctaagatc aattgaaaat tttgaaattt agtacgagga
    58621 tatttgcgca tagctagata tatattgaaa ataccgaata ttactaaaag caaaagtcca
    58681 gtattgttta cgatttgcca aacagcaaat aacagcagca gtgcttcgac taccacaaaa
    58741 aaacgccaag aactattcat cgctttttct ccctcctgac acattctcat ttcatcatac
    58801 ctattttttt gactgttggc tataggactt tagatgtaag atcgttcagt gaaacctgca
    58861 atactcaaag agaaaagatc tagagtgcgt catcagtaag atgaactctc tagatctttc
    58921 agtcagacta cgcttgttct actttgacga ttttaactaa catatcgcca cctggtgtag
    58981 caatggttac ttcatcattt ttacgtttgc caatcaatgc ttgcgcgata ggagaatcat
    59041 tggaaatctt tccagaaaac gggtctgctt cagcacttcc gacaatcgtg tactcttctt
    59101 catctccatc aggcaactcg atgaacgtaa ctgttttacc gatcgatacc tcatctgcat
    59161 ctacgccgtc attatcgatg atttgtgcaa aacggatcat attttcaatt gttgtgattc
    59221 ttccttcaac aaatgcttgt tcatctttag ctgattcgta ttcggagttt tcggaaagat
    59281 ctccgaaacc gcgagcgatt ttgattcgtt caatgatttc tccgcgttta actgttttta
    59341 gttcttctaa ttctttttct aatttttctt taccttctag agtcattgga taaactttct
    59401 ctaccatgtg taacgtctcc ttttttcgat aaaataccgc aaaaggtaat cttttgagga
    59461 atatagtcca aaaaagatta ccacgctttt cttaaaatgt aaatgttttt ctaaaaatga
    59521 tcaatgacta gcccctgttt ttctaagaat tatttacata tttttgcact agttccatat
    59581 gttcttcata cgttttagcg aaataaacat ttccagtgct cgtatctgct acgaaataat
    59641 aatagtcatt ttctgctggt tctaatactg ccttgattgc ctcttcgctt ggattgtcaa
    59701 aaggaccagg cccatagccg gtattggtat acaaattgta tggtgaatct acagctgtat
    59761 cttcatacgt caccaattct ttatgttctc ctaatgcata taaaacagag atatctgact
    59821 gaagtggcat atttgccttg attcggttaa agaagacttg tgcaatattt ttacgatcat
    59881 tttcttttac gccttctttt tcaactagag aagccaaagt taaaacttct tgtacagtca
    59941 aatttttctg tttgatagct gaataatagt tttgcatgac cgtatttgtt ttgttcacca
    60001 tctcaatcac aagatcctta agcgaagtac tcgtgtagta atcataagta gctgggaata
    60061 agtagccttc caaacggtat tttgtgttct cagctttaga ggcactttcc aacaattctg
    60121 ggaatttttg atacaactcg ttaaagaaag tctcatcatt tactaaatct aaaaattctt
    60181 tcttgtcttt accagttacc ttcgctaccc gctcagcaat ctgatcgatg tcgtagcctt
    60241 ctggaatggc aattttccca tctgcaactt ttgttggctc agaagtgccg ccttcttgca
    60301 gctgtttgcc aatctcgtca agagtcatat taggcgctaa ctgataatag ccagcttgaa
    60361 agccagttaa atttttaaat tttgtataat aattgaaaac aatcccgctt ttaataatat
    60421 tgtccttctc caaaatctca ccaatctgct tgttagaaga gccactcggg atctccactt
    60481 ggactaattg gtcatctgat ttatctaaag gcttcaaccc gctatctaca taacgataca
    60541 cggtaaatcc aagaaatccg ccaataatta ataatgtaag agcaatcact aaaatgatct
    60601 tcctcacaat cttatcttct tttttacgca gatttctccg cttgtcgatt atatctgctg
    60661 tagctgcctt actggcttct tttttatctt tacttgggtg ttgtttgttt cctgacaaaa
    60721 caactcctcc ttactatcat cttcgtaact ctgtttatta tacatgaaat ttctactgcc
    60781 gtgaattatt ttaccaaata ttatgtcaaa attgtaagtg gcaaatactc tttttgagga
    60841 ttacaatgaa aaatagttaa aaatggatag aaaggaatta acatgtcaaa aggaaaacat
    60901 cgaaaaaaac aaaaaatttc tatttctttt cttgtaattt tattattgtc tgtcagcttc
    60961 ttttcaacag gcatcattat aggaggtcaa ttaacaaaaa tacctcattc ccaaacttat
    61021 aaagcggagc caacacaact tacttccaat ttaacgcccg atgagcagag gcttgctagc
    61081 aaaattgatc aattactaca aaaatcagat tatatcggtt cgatttatgt tcgcaaaaat
    61141 aatcgcgtac tattagaaaa aggatatgga tatgcaaatc tgcgcgtaaa agaatcgaat
    61201 tcaccttcta tttactatca aatcggttcg attcaaaaag caatgactgc tttactgata
    61261 ctaaaacaaa tccaagctgg taagttaact tttgacacaa agttagcaga attttatcct
    61321 cagattcctg gaagtcagca aatcacaatc aaaaatatgc tttatatgcg ctctggtctt
    61381 catcgaactg cttctcccga caaacctatg tcggatagtg aaatcattca gttcgccctt
    61441 catcatttga aacttactga ttatgatacg tatcattatg aaccattgaa ttatacattg
    61501 cttacaggga tattgatcaa actaacgaat cagccttatg aaactcttct aaaaaaagaa
    61561 atcattcgtc cactccatct agaacataca gatttttatg acaatgtaaa aaattcgcct
    61621 caacatgctg tatcttacca gatgtctgca actgacgatt attcaaaagc attgatcgag
    61681 cctgaaaccg acatccgaaa tgaattaggt acaggaaaca taagtatgtc tgtttatgac
    61741 ttgaacaaat tctttaccag tgttctttct ggcggtatca taccaaaaga actacttttc
    61801 tcattgtggc aaaataatgg tgataaacat ccttacagcg gcggtgttta cagtggaaac
    61861 gattatatcc ttgctcaggg gaatatcaat cgattccatg cagtagtggc gatgaaaaaa
    61921 gacactaaag atgcaatcgt catggaaagt aatatccaat cggataagaa catcaaattg
    61981 ccggccactg atttaagaaa tcagatttat gaactgatgg aaggtgttca cttatcaaat
    62041 taaattttga cataatatta gttgtcatct ctgtatagtc aatttagtta gtactctatc
    62101 tgaaaagtag aaataaatta acaaatatta tctgtcctta ttatttgtta atttatttct
    62161 tatttta
//
LOCUS       DAPBAB010000010        59945 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPBAB010000010 DAPBAB010000000
VERSION     DAPBAB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 59945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.010"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(135..512)
                     /locus_tag="RD444_000820"
     CDS             complement(135..512)
                     /locus_tag="RD444_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548211.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="RD444_000821"
     CDS             768..1496
                     /locus_tag="RD444_000821"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDT7548212.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="RD444_000822"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="RD444_000822"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDT7548213.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="RD444_000823"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="RD444_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDT7548214.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="RD444_000824"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="RD444_000824"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDT7548215.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYELMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="RD444_000825"
     CDS             4631..6070
                     /locus_tag="RD444_000825"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDT7548216.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6093..7145
                     /locus_tag="RD444_000826"
     CDS             6093..7145
                     /locus_tag="RD444_000826"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDT7548217.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="RD444_000827"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="RD444_000827"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDT7548218.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="RD444_000828"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="RD444_000828"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDT7548219.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="RD444_000829"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="RD444_000829"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDT7548220.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="RD444_000830"
     CDS             10664..11578
                     /locus_tag="RD444_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7548221.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="RD444_000831"
     CDS             complement(11711..13276)
                     /locus_tag="RD444_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7548222.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="RD444_000832"
     CDS             complement(13269..14720)
                     /locus_tag="RD444_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7548223.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="RD444_000833"
     CDS             complement(14734..15750)
                     /locus_tag="RD444_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7548224.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="RD444_000834"
     CDS             15970..17058
                     /locus_tag="RD444_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDT7548225.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="RD444_000835"
     CDS             17112..18638
                     /locus_tag="RD444_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDT7548226.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="RD444_000836"
     CDS             18640..19845
                     /locus_tag="RD444_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7548227.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="RD444_000837"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="RD444_000837"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDT7548228.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="RD444_000838"
     CDS             21343..22767
                     /locus_tag="RD444_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDT7548229.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="RD444_000839"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="RD444_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDT7548230.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="RD444_000840"
     CDS             24340..25317
                     /locus_tag="RD444_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDT7548231.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25342..26985
                     /locus_tag="RD444_000841"
     CDS             25342..26985
                     /locus_tag="RD444_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDT7548232.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26993..28399
                     /gene="lpdA"
                     /locus_tag="RD444_000842"
     CDS             26993..28399
                     /gene="lpdA"
                     /locus_tag="RD444_000842"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDT7548233.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28561..28836
                     /locus_tag="RD444_000843"
     CDS             28561..28836
                     /locus_tag="RD444_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDT7548234.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28842..29609
                     /locus_tag="RD444_000844"
     CDS             28842..29609
                     /locus_tag="RD444_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDT7548235.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29777..31606
                     /gene="typA"
                     /locus_tag="RD444_000845"
     CDS             29777..31606
                     /gene="typA"
                     /locus_tag="RD444_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDT7548236.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31867..33639
                     /gene="recQ"
                     /locus_tag="RD444_000846"
     CDS             31867..33639
                     /gene="recQ"
                     /locus_tag="RD444_000846"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDT7548237.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33753..34055
                     /locus_tag="RD444_000847"
     CDS             33753..34055
                     /locus_tag="RD444_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDT7548238.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34058..35221
                     /locus_tag="RD444_000848"
     CDS             34058..35221
                     /locus_tag="RD444_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7548239.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35247..38675
                     /locus_tag="RD444_000849"
     CDS             35247..38675
                     /locus_tag="RD444_000849"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDT7548240.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38696..39820
                     /locus_tag="RD444_000850"
     CDS             38696..39820
                     /locus_tag="RD444_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548241.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39810..40247
                     /locus_tag="RD444_000851"
     CDS             39810..40247
                     /locus_tag="RD444_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7548242.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40231..40569
                     /locus_tag="RD444_000852"
     CDS             40231..40569
                     /locus_tag="RD444_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDT7548243.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40688..41248
                     /gene="rsmD"
                     /locus_tag="RD444_000853"
     CDS             40688..41248
                     /gene="rsmD"
                     /locus_tag="RD444_000853"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDT7548244.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41245..41736
                     /gene="coaD"
                     /locus_tag="RD444_000854"
     CDS             41245..41736
                     /gene="coaD"
                     /locus_tag="RD444_000854"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDT7548245.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41726..42769
                     /locus_tag="RD444_000855"
     CDS             41726..42769
                     /locus_tag="RD444_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDT7548246.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42928..43656
                     /locus_tag="RD444_000856"
     CDS             42928..43656
                     /locus_tag="RD444_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDT7548247.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43772..44017
                     /locus_tag="RD444_000857"
     CDS             43772..44017
                     /locus_tag="RD444_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7548248.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44014..44232
                     /locus_tag="RD444_000858"
     CDS             44014..44232
                     /locus_tag="RD444_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDT7548249.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44368..44907
                     /locus_tag="RD444_000859"
     CDS             44368..44907
                     /locus_tag="RD444_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDT7548250.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44900..47173
                     /locus_tag="RD444_000860"
     CDS             44900..47173
                     /locus_tag="RD444_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDT7548251.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47211..48257
                     /gene="holA"
                     /locus_tag="RD444_000861"
     CDS             47211..48257
                     /gene="holA"
                     /locus_tag="RD444_000861"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDT7548252.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48346..48600)
                     /locus_tag="RD444_000862"
     CDS             complement(48346..48600)
                     /locus_tag="RD444_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDT7548253.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(49000..49269)
                     /locus_tag="RD444_000863"
     CDS             complement(49000..49269)
                     /locus_tag="RD444_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548254.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49739..50194)
                     /locus_tag="RD444_000864"
     CDS             complement(49739..50194)
                     /locus_tag="RD444_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDT7548255.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50332..52260)
                     /locus_tag="RD444_000865"
     CDS             complement(50332..52260)
                     /locus_tag="RD444_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDT7548256.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52457..53290)
                     /locus_tag="RD444_000866"
     CDS             complement(52457..53290)
                     /locus_tag="RD444_000866"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDT7548257.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53482..54186)
                     /locus_tag="RD444_000867"
     CDS             complement(53482..54186)
                     /locus_tag="RD444_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7548258.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54209..54835)
                     /locus_tag="RD444_000868"
     CDS             complement(54209..54835)
                     /locus_tag="RD444_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7548259.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55161..57536
                     /locus_tag="RD444_000869"
     CDS             55161..57536
                     /locus_tag="RD444_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDT7548260.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57560..58051)
                     /locus_tag="RD444_000870"
     CDS             complement(57560..58051)
                     /locus_tag="RD444_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDT7548261.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58238..59158
                     /locus_tag="RD444_000871"
     CDS             58238..59158
                     /locus_tag="RD444_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7548262.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59312..59656
                     /locus_tag="RD444_000872"
     CDS             59312..59656
                     /locus_tag="RD444_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDT7548263.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59846..>59945)
                     /locus_tag="RD444_000873"
     CDS             complement(59846..>59945)
                     /locus_tag="RD444_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7548264.1"
                     /translation="KKNLGSLKAQQKNGAAIYREDEKEYYTKIKLM"
BASE COUNT        19669 a        10365 c        12873 g        17038 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaact gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
    25381 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    25441 gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca
    25501 ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta
    25561 gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa
    25621 tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg
    25681 tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt
    25741 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    25801 gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa
    25861 aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat
    25921 gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa
    25981 aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta
    26041 ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta
    26101 actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt
    26161 ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca
    26221 gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga
    26281 gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc
    26341 aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta
    26401 tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc
    26461 ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca
    26521 tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca
    26581 actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg
    26641 tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct
    26701 gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt
    26761 tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca
    26821 caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca
    26881 ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc
    26941 aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt
    27001 tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt
    27061 agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat
    27121 tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca
    27181 ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact
    27241 agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg
    27301 tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt
    27361 agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa
    27421 cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg
    27481 tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat
    27541 cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt
    27601 aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt
    27661 agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga
    27721 agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc
    27781 cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg
    27841 cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg
    27901 acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc
    27961 acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc
    28021 tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg
    28081 tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt
    28141 ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac
    28201 gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat
    28261 ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac
    28321 gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg
    28381 tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc
    28441 gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc
    28501 ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag
    28561 atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta
    28621 atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt
    28681 ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg
    28741 ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa
    28801 aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc
    28861 atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa
    28921 caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga
    28981 attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag
    29041 aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg
    29101 gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat
    29161 ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg
    29221 gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct
    29281 tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc
    29341 gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag
    29401 tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg
    29461 cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc
    29521 ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa
    29581 tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat
    29641 aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga
    29701 aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa
    29761 aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca
    29821 cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga
    29881 cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg
    29941 tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat
    30001 ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga
    30061 cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt
    30121 gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc
    30181 ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc
    30241 agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag
    30301 cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat
    30361 tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt
    30421 acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat
    30481 caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt
    30541 aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg
    30601 agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt
    30661 ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt
    30721 cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat
    30781 tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa
    30841 ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga
    30901 aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga
    30961 aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata
    31021 tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca
    31081 tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta
    31141 ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca
    31201 atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat
    31261 cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt
    31321 cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa
    31381 tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa
    31441 agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt
    31501 cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat
    31561 tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg
    31621 aagagagaaa ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc
    31681 tgtggagaag tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta
    31741 aaagactttt aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca
    31801 cgaaaggaga tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc
    31861 gggaagatga acgacttatt aaaacaatac tttggctatg atgagtttag acctggtcaa
    31921 aaagaaatta tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga
    31981 agtgggaaat caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt
    32041 gtgtcccctt taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata
    32101 cccgccactt ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa
    32161 atcgatagac agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat
    32221 tttatccaag caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt
    32281 atctctcagt gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat
    32341 caactgccaa atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct
    32401 gctgacataa aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa
    32461 cgagaaaatc tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa
    32521 tatttaaaga tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta
    32581 gaccgtctct atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt
    32641 ctaaatgaga atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg
    32701 attgtagcga caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc
    32761 cattatcaaa ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga
    32821 gatggtcttt ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag
    32881 ttttttatcc agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta
    32941 aaagcgatga cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat
    33001 tttggcgaga caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata
    33061 gtagaagtaa cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac
    33121 tatggtaaac aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct
    33181 ttaggttttg gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg
    33241 atgcagttaa ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat
    33301 cctgttttga aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat
    33361 cgaaaggaac ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa
    33421 gtgctgcggg aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta
    33481 ttttcagatt ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg
    33541 cttcagataa aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct
    33601 cggataaaaa atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat
    33661 gaataaaaac aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat
    33721 aattaaggaa ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga
    33781 tccaagtatt aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca
    33841 gtctctgtat ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct
    33901 tttcccggca agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc
    33961 tgatgttaag tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa
    34021 tttatgataa aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag
    34081 gcatctatta gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat
    34141 catggtatat agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc
    34201 agtcattaac cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa
    34261 aatgaaaaca gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac
    34321 gattttgctg ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct
    34381 gcaaattgca ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta
    34441 cctgtctctg actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt
    34501 gaaacgtcca cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt
    34561 tggtaatgcg gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa
    34621 ttatgtgtat actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg
    34681 gctgatcaat atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg
    34741 atttgctatt taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa
    34801 cggctactat gccatgttca acggaggact atttggcagg ggattaggta acagtatcca
    34861 gaaaaaagga tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga
    34921 actgggagtg attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat
    34981 tgtccttgta ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg
    35041 cgcaatgttt ttgatccaag tatttgtcaa tcttggagga attactggcg ttattccttt
    35101 gacagggatc acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat
    35161 ttgtgtcggc tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata
    35221 ataaaaaaag atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa
    35281 tcgctgtacg tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg
    35341 cagaagaaga tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac
    35401 aaggaaaaaa accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag
    35461 aatgcggagc tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg
    35521 cgcaacggtg cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata
    35581 tttttggaga taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc
    35641 caggcacaga tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg
    35701 gttatccgat tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc
    35761 acgatgaaaa gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct
    35821 ttggtagtga tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac
    35881 agattctcgg ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc
    35941 agcgccgtca ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac
    36001 gtcaaaaaat ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag
    36061 gtacagtaga atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg
    36121 tccaagtgga acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac
    36181 tgttgattgc tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa
    36241 ttaaattaaa tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact
    36301 ttttaccaga cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc
    36361 tggatgtcgg aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag
    36421 taaaagtatg tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct
    36481 tgaaagaatt tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca
    36541 cacatcctga attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat
    36601 tatttgaatt tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa
    36661 ttactgtgaa tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac
    36721 gtatgcctaa agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg
    36781 cgaagggtgc cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg
    36841 atacgacatt tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt
    36901 tcaagcagat tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt
    36961 gggggggagc aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt
    37021 tgagaaaaat tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa
    37081 atgccgttgg gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag
    37141 cagcgcaagg aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg
    37201 aaaaaagtat ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata
    37261 ctggcgatat caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg
    37321 caaaggaact agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc
    37381 ttaaaccgca ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa
    37441 tccatctgca tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa
    37501 aagcaggagt agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc
    37561 ctagcatgag cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga
    37621 ttgaaaatgc acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt
    37681 ttgaaaacgg cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac
    37741 agtattcgaa tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa
    37801 tcaaacaaat gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat
    37861 catctaaagt agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag
    37921 atatttatga aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag
    37981 gagaactagg gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag
    38041 gcagacctgc attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag
    38101 taaaaaaaga attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct
    38161 atttgatgta tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg
    38221 ttacattact ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg
    38281 tacaaatcga acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata
    38341 tcgaagggaa tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca
    38401 aagataattc gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag
    38461 aacaaattgg agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata
    38521 acgttaaaaa aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg
    38581 aagcacgttt tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgt
    38641 ctggtgacct tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa
    38701 aagactgggt ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc
    38761 agtcttgttt ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca
    38821 aatgacaacg tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa
    38881 accgatcgat cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt
    38941 tggttttgaa acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat
    39001 ccaagacagc agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc
    39061 gttttattta gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt
    39121 cacttttgag taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta
    39181 tcgtccgcta attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac
    39241 aaacggccta ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata
    39301 tcaagtgttt ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc
    39361 aatcaatcgc gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga
    39421 cgaacttccc atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca
    39481 tgattttttg acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa
    39541 atcgctaggc agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa
    39601 gttaatcagt aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga
    39661 cgctactttt tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga
    39721 cccgggaacg gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca
    39781 agaagaaaac ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg
    39841 aggatcaatg cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc
    39901 ttgaacataa acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct
    39961 tgtctaagaa agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc
    40021 gtgaaaaaaa acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg
    40081 cagctttccg atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac
    40141 ttgccgaaga agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga
    40201 aaaaacactt tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata
    40261 acaaaacgac gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa
    40321 aagttcggct tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat
    40381 gaagagacat tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta
    40441 gaacgttctt accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca
    40501 gtattaaaag acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa
    40561 catctttaaa aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga
    40621 tttttgatca ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag
    40681 tgagtagatg cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg
    40741 ggcgaacacc agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc
    40801 atattttgat ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga
    40861 agcagtatct agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa
    40921 aatcatcaaa gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact
    40981 tgatgcggac aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt
    41041 tcttgatccg ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact
    41101 gggacttttt tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc
    41161 agaaactgtg gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac
    41221 tatctataga aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct
    41281 tttacaaaag gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta
    41341 attggtgttt ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca
    41401 ttgatcacaa aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa
    41461 ttgacggtag aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt
    41521 atcaaagact ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa
    41581 ttggaaactg tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta
    41641 aaagaagtac tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa
    41701 gctttagctg gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat
    41761 tatcatattt ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga
    41821 atcaccggga gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa
    41881 aggttctggg tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc
    41941 tttaaaagct aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg
    42001 ggcgacaaat gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc
    42061 agcaatcgaa caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt
    42121 atatgtcatg agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac
    42181 agtaacaaaa gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa
    42241 aagccaaaaa gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac
    42301 agcttctggt gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct
    42361 tactgaccat accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg
    42421 tgggccatca gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa
    42481 cttgcgacat ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg
    42541 ccgaatcgga ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt
    42601 ctttgcacca gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac
    42661 caactacgaa gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc
    42721 agtaaaaaca gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat
    42781 taaagagggg ttgtgacacg agttctgtca caacccctct ttttcgtcaa aaatcttcac
    42841 gataaaaata aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg acgattttgc
    42901 cagagctcac tgtttcttca attttaaatg aaaggagtaa tgaatgattt ttactggtca
    42961 ttctttgaaa aaagaacaat gatagaaaaa atcagcaaga aaatactagc ttcacccaaa
    43021 ttgatcattt ggatgtctat gatagttctt atcttttgta tcgcaagtgg ttttttatta
    43081 tcaaatttgc tattttatag agaaacagaa gagactgctg caaaagaaat actttcttcg
    43141 tttagttcca gtggagaaga tccatcagct gaaggaaacg atgtagaaga ggatgctcgt
    43201 ccaaaggtca tgtacaccga tatcaaagga agtgtgaagg agcctggtat ctattccttt
    43261 tcttcagaag aacgggtcta tgatgtacta aagcgtgcag gtggattatt agaagaagcg
    43321 gatagcgatc gaatcaactt ttctgcgaag atagaagatc agcaagtcct atatatccct
    43381 gctgtaggtg aagagctgcc agaacatctt gatcagttgg catcctcgga aggaaaacag
    43441 cctacagcag attccgagcc tcccaaaata aatatcaata cagccactcc atcagaactt
    43501 cagcagattc ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa
    43561 aacggatcct tccagaaagt ggaagacctg caggagattt ctggtatcgg tgaaaagaca
    43621 gttgaaaaac taaaaaactt cgttacaatt aaatagaatg attcaaaatc gactggcacg
    43681 agcaataaat ggacgctaat ggatactaag aataatccaa ttctaagagc ttggcaggac
    43741 gaggaggtag tatttttgga taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa
    43801 accaatgctg aatgtctatc tccaaaataa aatatctggg attcatatta tgaatacggc
    43861 tgtcagcggc aataacagtc aagctctacg agaacgcttt gccaaagatg tacttagcta
    43921 tacagcagat aaagtattta ttctcattgg gacgaatgat ctagcagagc ataaacaact
    43981 ttctaaagaa acgtaccaaa aaatctgcag tggatgatag ttcagctgaa aaaagactat
    44041 agttctgatc atgctttctt tatcacgcca ccagcagtcg atgagacaaa acaaaaatat
    44101 cggaataaca agttgatcaa agaatatatc tctgttctca cagaagtagc aaaacgccat
    44161 gattgtgaag tatcgatttt tatacggaat ttcttgaaca aatggattcg aagaattttt
    44221 ataatgggct aatggatgac ggacttcatt tcggcagtga agggtaggat cttttgtcag
    44281 acaggatgat tcgttatatt taaagaatat tttgtttaag caatgagtac tttttaatca
    44341 acttgaaaaa acggaggaat ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg
    44401 aataaagaaa aagttactga taagtcacat aatgaacgca ttccttggga ccagtacttt
    44461 atggctcagg cagtactgtt atctttacgt agtacgtgta cacgtttaga agtaggagcg
    44521 acactcgttc gagaaaaaag gatcattgct ggcggctata atggagcagt cagtggagat
    44581 gtccactgca tagatgaagg gtgttacgtc gtggatggac actgcttgcg aacgatccat
    44641 gcagagatga atgcactcct acagtgtgca aaacttggta tcccgacgga aggttcagaa
    44701 atctatgtca cacattttcc atgtttggcc tgtacaaagg ctttgttgca agctggtgtg
    44761 aaagggatca attatctcca tgactatcga aatgatcctt atgcccaagc gttgatcgaa
    44821 caattaggtg tatctgtaca ccaagttgct ttagactcac agcatttttc aaaacttcaa
    44881 aatgaattaa caggaaaaca tgtctagcgt tttagaaagg catgttcgaa atcggttgat
    44941 ttttccggca attacgtcga tgattatctg tttgttgtat tacactgaca attggggtta
    45001 tcgtttgctt cttgccggaa tttttttatg gttttcgcta aaagaaggac gttttatcgc
    45061 gtacttgtgt attttgtgta gcataacggt atccatttct tataggctac aagagtattc
    45121 atttttagag gaaaaaacaa catctgcgca aattactgta tttacagata cgattcgtgc
    45181 aaatggtaat tttttgacaa tggaaggaag gttatcagag aataaacaaa agctccagct
    45241 ttcttatcaa gtaaacacag aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg
    45301 aatagaattg actgctgaag gcgagttcat ctctccagca aaaaagcgga atctttataa
    45361 ttttgaccaa gaagcttact tcaggaacca tagaatttct ggacggttcc aaattcaaaa
    45421 gattgaacag atcaccatgc aaaatcactg gcgaaactgg cttcagagaa aaagaggatc
    45481 ttttatctcc tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt
    45541 attatggggc tataaagatg caggattcag agaaccagaa gaaattttta gggagagtgg
    45601 tttgctgcat ctgttcagtc tatcgggtct acacatccaa ttttatttag ggtggctgta
    45661 ttatttattt cgacgtatcg gatggccact acacgtcagt attattccgt tgagtctact
    45721 aactatctgt tatgtttcta ttgcaggaag ttctatttct gttttgcgtg ctagtttgct
    45781 gtttttattg cgattgctag tcaaattgct gaataagcat tattccacca tggacttatt
    45841 tgcagttgtc ttttggcttc ttttactatt tgatcctcga tttctttttc atgcgggtgg
    45901 acagcttagt tttttcatga gttttttgtt tatcctgatt tcttttgatg gagattggtt
    45961 gaaaaaaacg gcctctcacg tcctgtttac tatgatcacg atgccgttga ttgtatggca
    46021 tttttatgag tggcctattt taggtagtct ttttacatta ctgattgcac cggtattcaa
    46081 gtttcttttt cttcctggtg tgttatttct cacactcttc aatcgctggc tgccggaaac
    46141 tttacttttg ctgatagaag agggattaat cttatttgag cgaataatcg atttttcttc
    46201 agggtcgtct ctcattatag gacgtttgcc ccttgtgcta tctgggatgt atattttcgg
    46261 attactgatt tgtatggata ggttgaaaga acaacctgtg aaacacttgt tttatgttgg
    46321 tcttttttct tttatttttt tgactttgcc atttttacgc ttttcttcaa cgatcgcttt
    46381 tatcgatgtc ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa aagaaacgat
    46441 tttgatcgat actggaggtc agttacactt tgaaagagaa aagtgggcgc aaggtatgat
    46501 acgtcctcct gctgaaagaa atatcgttcc atatctaaaa ggtcagggga taagtgtaat
    46561 cgataaactc atcttgacac acgacgatac ggatcatgtg ggagaattac ccacgttatc
    46621 tcagcatttt actatcaaaa aaatttatat cggttggggc gctggacgaa atgagccatt
    46681 acaaaggcat ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg
    46741 ggacaggatc agcggttatt atgatcttta tgtattgact ccttttgaaa agggagaggg
    46801 aagaaatgaa gattcgattg gtctatggat ggaatataac aataggcgct ttttattttt
    46861 aggagatttg aatcaagcaa tggaaaaaca attgcttttg atttatccta atctgaaagc
    46921 cgatgtagtc aaattaggtc atcacggtag ccgtacgtca agtaacacag attttctttc
    46981 ccatattgaa gcaaaacatg ggattatatc ctgcggcgta aataatcgtt atggtcaccc
    47041 gcacagcgaa gtattggaga cactagaaga aaatcagata aaaacatggc gaacggatca
    47101 acaaggaatg atcacctatc gctggcatcc agtttttcat cctcatgggc tagtggaaac
    47161 aatgatagac tagtaattct catctgctca tggtaggata atttttgaaa gtggtgagta
    47221 atttgagtat acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact
    47281 tgattttagg aacagaaaag tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa
    47341 tccaattaga gggcgaagat gacttaaatt ttttaagttt tgacatggaa aatgcaagtt
    47401 tagatgaggt agtagctgaa gcagagacgt tgccgttttt cggagatcaa cggttagtat
    47461 tcgtagagaa tccttatttt ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata
    47521 ctgatttatt gacggcttat ttgaaagaac cattagaatc gactgtatta gtattctttg
    47581 cgccatatga aaagttggat gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa
    47641 ttatcatcaa tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg atgaatacct
    47701 tagagaattc ctcaatcaaa atggaacgaa gtgcaattga tttattcttg aggttgaccg
    47761 acttagattt atccaaaatg atgagagagt tacagaaatt ggtcctttat ggacaaaatc
    47821 aacagcaaat cacggtaaga gaagtagaag aattagttcc taaaacgtta gaacataata
    47881 tttttgacat gacccaatat attttaaaag ggaagacgga acaagctttg cgtttatacg
    47941 aagatttagt tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat
    48001 tacgtttatt tttacagaca aaatttcttg taaagattgg ttatcagcag gcaaatattg
    48061 ctgaaacatt gaaaatccat ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat
    48121 tcgatgaaca tttgctggta cagttgtttg atcaattagt tgagatggat tatcaaatca
    48181 aaacgggaca aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc acagggcagt
    48241 tgttaagaaa tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt
    48301 tgtccttttt ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt
    48361 tttgcttaaa cgagatttgt cgcggttagc tttgtttttg tggattagac cttttgattc
    48421 agccatatca atagctttaa cagcttcttt atataacgca tctacgttgt cagcacctga
    48481 agctacagca tcttcaaatt ttttgattgc agtacgcata gcgcttgttt gagatgaatt
    48541 tttcacatta gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa tgtttggcat
    48601 tagtttcacc tccgataact taagttctta cgcgtgttac cgcataattc aacattcatt
    48661 attataccta aaggcagcgt gctttgcaat atgaacatga caagtttttt tccttaacag
    48721 tcttttatta ggattttttt tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa
    48781 gacgctattg tgggaggcaa tagcgtcaaa ccaaactaga tttttattta tctatttaaa
    48841 caaagtttga atgggaatgt ttggtactaa gttattatat aacaaagaga accgtttgca
    48901 aagttttttt tgaaaaggga tacgattccc tgttgttctt tgtttttcta ttaagaaact
    48961 gcagttattt tataaccagt atcctttgat cagtaaaggc tattctggtg taccgattat
    49021 tttaatgtat tcatcgatat tttttatcaa catttttttt atgaccaact ggttccgacg
    49081 gttccatttg ccacttactt ccagcacata tttatttagg ggcaaacgta agatatattt
    49141 tgccaaatag cgatctttaa gcaggccatt gatcacacgg tcctcaagct gcaagttgat
    49201 cccagtcagc atcattggct gttcttgtaa aacgtacgta tcaattaaaa gaccttcaaa
    49261 agtttccaca agcgtcttcc tttcttttca aagtaacctg aaaacaggca ctaattcttc
    49321 cattttgata ttcgttgaag tggatgcgat cattctttct tttgtccact gtaatctagt
    49381 ataacaaaaa agagcagaag agccaaaaag aaaaaagagc atcgaattct ataggaagtc
    49441 tcattggcac ggcctaaatt attacaaaac attgattaaa aaaagcccct gcatcgcagg
    49501 agcatcccct atatcgtttt aataggtcaa tcaaaaagca gtatcaaaca cattttgtat
    49561 caaatcagga aataatagat aagctaagcc cagaatcaaa agtaccgcta gaagaaacca
    49621 gccgattgtt cctatgaaat actttaattt ttcgaacatt gtatcacctc gttgtcagta
    49681 tatcacggat cagtaaattg ggggtgttaa gaatctccta aaatagacaa aatttgttct
    49741 attttcctaa agcaaatact tgtaagatac agtaagtgat accagaagca atgaccggtc
    49801 cggcggcgat tcctcttaag aagacaacac ctaaaatcgt gccgaaaaca agtgcaacag
    49861 tgatttgtgg atcagaagca atcaatccta cacccttgga agataagaca gccactagcc
    49921 caccgcaaaa aacagctacc cagccaatag gggatttaaa ggcttctaat agatcacgta
    49981 gaccgatttc tcctgttgcg atagggatta gaatagccgc tgtgatgatc gtcaccccaa
    50041 tattgattcc tttgccttga agccaaggga aaaaggaacg ggaggctggt atgagcttca
    50101 taatcaaaac aaaagctgcc gctatcatca gactttgatt tttagctaaa attgctacaa
    50161 gtaaaataat tcctaaaaaa agccaacttt ccatgaaata tattcctcac tttcttcgaa
    50221 acaatcataa catgttttta gaaagttaca aaagtaggaa ttcatccttc aagacctatt
    50281 cataagctgt ttttttcata aaataaacaa aatcataaaa aaacactttg cttatttgac
    50341 agaagacagc cgcgctttgt atagtggaca agacttacat ttttttgaag aatcagaaag
    50401 atcaggtaaa ggtcttagta aacgcaagcc attcagaata accaatattg tgcttccttc
    50461 gtgtcctaca actccaagag gtagatttaa aaattgcagg aagttagaag caatcaacaa
    50521 gagaataaca agaatcgaaa aaataatatt ttgtttgatg atccgccgca tttttaaaga
    50581 tagccgatga ctgtagacaa atttatttaa gtcgcttttc attaagacga tgtctgcgat
    50641 atccatagcg gcatctgtcc cttgccccat agcgacacct aaagaagcgg ctgctaaagc
    50701 aggcgcatca ttgactccat caccaaccat cgcatttact tggtaaagtt cattctcttt
    50761 tttgactagt tgtgtttttt cttccggagt acagcttgca tagtaatgtt caatccctac
    50821 ttgttcagca atggcctttg ctgtgccaga atggtcgccg gttatcatac ttgtatggat
    50881 gttttgggat ttaaagtagg aaattacttg ttgggtattt gcttttggaa tatctagcag
    50941 acctaaaaca gcaattaact gctgatcttt agaaagataa atcacggttt tgccttgatt
    51001 ttccaaacgt tcgatcattt ccgcaatctc tttgctgatt atcatcgtct caggatcata
    51061 tgcgtgcttg ccaactttcc attgcgcacc taaataggta gtttgtaatc caaaaccagt
    51121 aatttcttct attggtaagt attggatttc ttcaggtatt gtgcaatcga aatattgcaa
    51181 aatcgcatga gcaagtggat gcgtcgtttt ttgttccata gcaacaagaa gttgttgtgc
    51241 caaatccttt tcatcaaaga aaaagctgtc agttacaact ggttttcctt tagtgagcgt
    51301 tcctgtttta tcaaaggcaa tcgcttttaa ttcagaaagc ttttctaaat ggataccgcc
    51361 tttgattaaa atcccatttt ttgcagaatt tgaaagagca gctagagaag ctggtgtagc
    51421 agatgcgaca agcgcacaag gagaagccac aactaataat accattcctc gataaaagct
    51481 ttcatcccaa ctccaaccta ggaaaaagtg ggggagaagg atcattagag gaacagcaag
    51541 caaaatcaat tttacataat tattttcgat tcgttcgata aatgaagctg tttgagttgg
    51601 cgtattctgc gcttcttcta ctaattggat aatcttagca aagacagttt cggaactcgt
    51661 ttttgttact cgaatcgtga tgggatttcc ttgattcagc gttcctgcat acatctcatc
    51721 gcctaaatat ttttctacag gcacagattc accgcttaat gcagactcat cgatcgttgc
    51781 ctgtgtgctt tctaaaaagc catctatcgg aacagctgct cctttagcga caaataccaa
    51841 gttatccacc tttagttgag aaacatctac ttcttgcatt tgtccatttt cttttaataa
    51901 ttgggctttt tgtggttgaa gattcatcaa agcagtaatt tctttttgac ttttattagt
    51961 tgtgtattcc tctaatgcac cgcttaaaca aaagatgaat gtcaacattg ctccttcaaa
    52021 gtaattaccg atcaaacagg caccaatagc agctaaagcc atcaataaat ccacattcag
    52081 tgtccgatca ttccacaatt ctttcaagcc ttccgatgtt tgtttatatc caccgatgat
    52141 aatggcactg atataaaaaa gtggggtgat ggtaagagag aatcggtcga agataaaacc
    52201 gaagatcata aataaaaggc agccgattgt tgctgcaaaa gcttttttct gattagtcat
    52261 cgttattaga cctccttgtc aaaacaagga tagcataata aaaatgtaat tgataataat
    52321 aacagctaaa tgagaacgat aatggttatc atttggtgat taagcttgat gttgttttta
    52381 ttatttataa tgattattat taaagaaaat aaaaaaccag aacatttctc aaataagaga
    52441 agtgttctgg ttttttctac attgcaataa accaataacc aatcaaaatg ataccaagga
    52501 taatacgata ccagccaaaa gcggtaaagt cgtttttctt caaatagctt agtaagaatt
    52561 tgatagctaa gacggaaacc acaaaagata cgaatgttcc tacaagaaga acaacagttt
    52621 cttccatttt gaatgaattc ccttcagcta aatatttgac gatcttcaag ccgcttgcgc
    52681 caaacatgac aggaatccct aagaaaaatg aaaactctgt agcgacaaaa cgcgaagcac
    52741 caatcaagat agatcctagg atcgttgcgc cagaacggga tgttccggga acaagtgcca
    52801 agacttggaa taacccaaca agaagagctg cttggtacgt aaagtcattc agagaagtcc
    52861 aacgaggtgt tttgtttttg ttgcgttttt cgacaataat aaaggcaata ccgtaaacga
    52921 tcaaaacaat ggcaactggt aagaacttgt ggaaatttgc ttctaaccaa tcatccaaag
    52981 gcaaaccaat gatgatggct ggtatgcaag caacgatcac tttaaaccaa agtgtccacg
    53041 tatctttttt ttctaccgtt gatttacttg gagaaaatgg gtttaacttg tgaaaataaa
    53101 gaacgacgac agccaagatc gcccctaatt ggataacgac attgaacatc gacataaatt
    53161 gtgaactggc atccagtttg atgaattcat ctgctaggat caaatgacca gtactactga
    53221 tgggcagcca ttccgttatt ccttcaataa taccgagaat cactgctttg ataatatttg
    53281 caaaaaacat aaattccttc cttcccaatt aagatacatc ttatagtata ccgttatttt
    53341 ttgagaaacc caatctgttt tttaatatta agaaaaactt gagaaataaa gcagataagt
    53401 acacgaagag aaaaaagaag acaaagccct cgttcagact ttgcctttta tgattctctg
    53461 gtaaaaatta aaatatcccc attatagaaa cgagtcgcga ctatgtgttt tcccgttatc
    53521 gatataagac gtatcattga tgccgtggat agaaaatcga ccgtttgcat aagtgacctt
    53581 tgttacgctg ccatttaaaa gcccagcacg aacaggctgt ttaggatcga tcagattaag
    53641 caaaaaggcg atcgtcaaac catgagatac gactaaaaca tttccaccac catttttttc
    53701 gcagtgatgg gcgatatctt caaatcctgt ccatacacgg tctcgcaatg tttcataagt
    53761 ttccgcccac ccagcggtat ctaagttgta tacagcatta gcaatttgtt caaaagaaac
    53821 ttctgtggta aacatttcat catatgtttt aaaattcaat acacgaggga tcacacccca
    53881 catttccatg tcatatccac cttcaagaga cccgaaacac cattctctaa tccttttttc
    53941 aataaaataa ggaatttctt ttcccttaga atgttctcct agaataatac gggcagtttc
    54001 gattgcacga ccactgtcac tagaataagc aaatgaaaag tcgacatttt ttaacccgcg
    54061 acctaaataa tgaatgcctt cttgtccttg ctttgtgagg ggtgtatcac accagccttg
    54121 tactcgttca attgtattga acatcgtttt tccgtgtcgt acgatataaa gtgtagtttc
    54181 tgtcatttta ttctcctcct aagtcgtttt aacgattgaa aaaaggattt gtcctttttt
    54241 cctgttcaat cgtcgttgca tcgccatgtc ctggatagac tgcaaattca tcaggtaata
    54301 caaacagctg ggtccgaata ctatgaagta attgttctaa attccctgta tacagatcgg
    54361 ttctaccgat acttcctcta aacagggcgt cgccagaaac gacaaaatca tcaaaaataa
    54421 agctgacgct tcctatagaa tgccctgggg tagggacgac gcgaaaagac atgtttccta
    54481 aagtataagt tttcatttca aattcatatt ctgcaggcga gacaataaca tcagcaatat
    54541 catcatgtct accgagtcca gaaagattca aaatcggatc acctagccat ttttgctcta
    54601 gtgggctgac ataaactggg atttgataat acgcgcgtaa aggttccact gcaccgatat
    54661 gatcgtagtg agtatgggta agcaaaatcg ctaccggctg ttggtttgtt tttttgattt
    54721 cttgtttgat tcgttccgca tcttctccag ggtcaatgat caaaagtgct tcttcgttgt
    54781 agaccaaata acagttttct tcaatcattc ctgtttttaa tctttcgata tgcatgtgca
    54841 ttcctccttg atagccaaaa cttctttcat ccattagtat aacgcattca ttgctaggaa
    54901 acaaaaacag gtacttattt ctctttactt atctgaatta tcagactata tgaaaagtat
    54961 ttggatattc taaagagaag gaaagtaagt tttcatagca ttttaatatg tccttgttac
    55021 attagattca tccaaaaatc caaattctat atcctatatc cctgcctaat cagcagggtg
    55081 tttttttatg tttggaaaga gagctctttt tgctaaacta ggttttttca ggtaatctta
    55141 gagagaaaga ggtggtggaa atgatcaaac agtcaaaaat atctgtgcgg cacttagtag
    55201 aatttatttt gcgacgtgga agtatcgaca atcgtaaaaa aagtaaccat actgcattag
    55261 aaggagcgag aatccaccgc aagctccaaa aagatgcggg agaaaactat gaaaaagaag
    55321 tctttttaaa aacaacagtt caattagatg aatattacct gatcgttgaa ggccgtgcag
    55381 acggtatttt caaaagagac ggtatttatt atatagatga aatcaaaacc tccgaaccac
    55441 gatttgaaga tttagaacca gaacaagtgg aacttttctt tcatcaagca agggtgtatg
    55501 cctatatcta tagtcaagaa catgatttac aagaaataaa tctgcaattg acttacttcc
    55561 agacggcaga agaagtgatc acgagaaagg ttgaacatca gacaagagag cagttagaaa
    55621 ctttttttaa aaagctgact gaagattata aagaatggct tgtttttcaa gaaaactggc
    55681 gcactgtacg caatgcctct ttgatggctt tgaaattccc ccatgatgag tataggaaag
    55741 gtcagcgtga actagcggta gctgcttata aaacgatccg tacgaaacag aaattgtttg
    55801 tagaagcacc taccggaaca ggaaaaacaa tctctacgtt attccccgct ttgaaagctg
    55861 taggggaaga agagggagaa aagatctttt atctaacggc taaaacaata actagacaag
    55921 tagctgaaga tgctatgaca gcattaaaag atacaggagc tgaagtaaaa agtgtcactt
    55981 tgacggcgaa agacaagatc tgctttttag atgagacgac ctgtaatcca gatcagtgcc
    56041 catatgccaa tggttattac aatcggatta atgaaggttt atgggatctt ttgaatcatg
    56101 agaatcagat cactcgtgaa gtcatcgaaa cttatgcaag aaagcataca ctttgtcctt
    56161 tcgaactatc attagatgtc agtatctggt gtgacgtaat cattggggat tacaattatt
    56221 tatttgatcc aactgtttat ttaagacgtt tttttgaaga tgaaaaaaac gaggactatc
    56281 tgtttttgat tgacgaagca cataatttgg tgaatcgttc ccgagagatg tattcggcag
    56341 aactatctta tgaaaaaacg aaacgaagta aggaagctat accaaaggaa ttcaaaaaac
    56401 ttcatcgccg tttcaacaaa ctattaaaag aatttgattc gatccgagaa atagcaaaag
    56461 aagatcattg ggattatcat catcaaaaag caccggctga atctttagta aaggcaggtt
    56521 atcaattaag cgaaaaaatt aaagaatggc tggcagaatt tcctgagcac ccatcacaag
    56581 aacaattgct tccttattat tttgatttgc ttcatttttt gaaggtcagt gaattttatg
    56641 atgatcacta cgaaacaaca gtcgaaaaaa cgtatcgcga tctgatagta aaagagtttt
    56701 gtattgatcc aagcttgttt cttgagcagt cgttggataa aggaaaaagc agtattcttt
    56761 tttctgctag tttttcacca ctctcttatt atcaggaaac actgggggga caaaaaagtc
    56821 tggcttacaa actgccgagt cctttcccag aaggaaacca gcaagttttc atcgcaaatt
    56881 acctagagac gacttatcga aaaagggaac acagtctggc aaaattagta gagacgattc
    56941 atgcttttgc tgatggaaag gtcgggaatt atttcgtttt ctttccttct tatcaatatt
    57001 tagatcttgc ggtggaagca ttcagacagc aatatcccga cagcaatgtg ctaatccaag
    57061 aaacggacat gaatgaggaa gaacgcgaac gatttttaac aaaatttcaa acaaatcccg
    57121 ctgaaacatt gattggattt tgcgtacttg gcgggatttt ttcagaagga atcgatttga
    57181 gaggggaccg attgatcggc agtatgatcg taggtgtagg tttgccgcag atgaatcatg
    57241 agcaggaact gatcaaatca tattatgatg agaaggaaca ccttggattt gcttacgctt
    57301 atcagcttcc tggaatgaac aaagtcttgc aagctggtgg tcgagtgatt cgtggcatgg
    57361 cggatcaagg aatcgttgta ttggcagatc aacgattttc tagtttttct tatcgacgtt
    57421 tattcccaca acattggcaa acagcaagag aagtacgaag tgttggggaa ttggaagaag
    57481 gaatacagcg tttttggtta tcaaaagaag cagtaaatca aaaaaacgag gaatgacaga
    57541 cgtcagcctc gttttttgat taacgatagt tagtgaattg caactcgatt ggtaagtcaa
    57601 gtttacgtaa taaagcaatc acagcttgta gatcatcacg gttcttacct gtcactctaa
    57661 tctgatcttc ttggatttgt gcttttactt tcagtttttc atttttgata gctgtagtga
    57721 ttttcttcgc gttttcacga tcgattccgt tgaccaaatc ggcagtttgt cgagctttgc
    57781 cacctagagc gtgttctgat tcggaaaaat ggatattttt gattggcaca tttcttttga
    57841 tcagcttacc aaataataca tctttgatct gttcgatttt gaaatcatca tcgctcaata
    57901 caactagatt gctgttctct aatttgattt ctactgtaga atccttgaag tcaaagcgat
    57961 tggacaattc ttttgttgtt tgctgaatcg cattttttac ttcttctgta ttgacttctg
    58021 atacaatgtc gaagctagca tcttttgcca taggaaccct cctgatatac atataaatat
    58081 ttaccataca tctatcttat cataggatta gggcagattc ttacctgttt gctaaaattc
    58141 ttctcatttg agttcgaagt attttatttt aatagataaa ctgttattct tacatttgga
    58201 ataccaaagt aggggagagg tgcttgtgag taaaattatg actgcgtatc ataaaaatga
    58261 aagattaaag aaaatagtgg ttattttgat aaccggattg accgctgcgg tcgctttaaa
    58321 ttattttttg attccagcca atgtcttttc tgcagggatg aatgggatcg ctcaaatcat
    58381 ttcctcgctt ttatatgaat ggtttcatat caagatagac acaggaacat ttatttttct
    58441 tttgaatatc cctgtgtttg tattagggtt tctcaaagta ggaaaacgag caacgatcct
    58501 tagttttgtg aatgttgtat gtgtatcggt gatgacgatg ctgcttccag tcggacagat
    58561 cactgataat attttgatga atgccctagt aggcggtgtt ttattaggtc ttggcgtagg
    58621 attatcttta aaaatgggct ttacaactgg tgggatggat atcatctctc ttgtactttc
    58681 acaaacaact ggaaaaacag tcggaaacta catgctgatt ctaaatggta tcattgtagt
    58741 catcgcaggc ttcttcttta actgggaaag tgccttgtat acgattattt ccatttatgc
    58801 aatgactcaa gtaattgatg ccatccacac gagtcatcaa aaagtaacag caatgatcgt
    58861 taccgtcaag ccagaagagg taacagaaga actagcaaaa cggatggtac gtggaatgac
    58921 attactgccg tctgtcggtg gctattcaaa aaaacaaggg aacatgatca tgatggtcat
    58981 cacccgctac gaaatgtatg atcttgatca gatcgttcat gaaatcgacg acgatgcatt
    59041 tatcaatatt ttaccaactc attcagtatt tggccggttt gcaaatgaaa acgaacaaaa
    59101 aatgtatcga acaactggcg tattgccaga aataaaaaat cgttcacaaa aaaaatgagg
    59161 aatcaggttt attttagaat aaatttaatc tattacatct aataagaaca tttttataaa
    59221 tttaaacgag aatcttactt ctaaattctc atttacaagc agctaaaaaa agagtatgat
    59281 aaataaaaat cgtgttaagg agtgtcgtac gatgagcgaa gcaaaccaag aacggtctgg
    59341 acaagaaata gaactaatca aagaacgtat tcttgctgcc ttggaaatgg tgattgatcc
    59401 tgaattagga atcgacatcg tcaatttagg cttgatttat gatatagaat tcaatcctga
    59461 aaatggagaa acagtcatta agatgactct aacaactatg ggctgtccat tagcagatat
    59521 tttaacagaa tctatccatg acgcattaaa agaagtgcca gaagtaacga aagcagaagt
    59581 caaactagtc tggtatccag catggacgac agataagatg agccgttatg cacggattgc
    59641 gttggggata cgatagaaat cttggctgac tagctatgag aagcaaatag gaaaaacaaa
    59701 aaagcctttt atcctttcta gaagaaaaga gataaaaggt ttctttgttt ataaaatttt
    59761 gtctgctata ttataagtta attaaataaa tgtgttctat tgataaagga cattcaaatc
    59821 cctttccttt tggaatcctt cttttttaca tcagtttgat tttcgtatag tattctttct
    59881 catcttcacg ataaatcgca gccccatttt tttgttgtgc tttaagactg ccaagatttt
    59941 tttta
//
LOCUS       DAPBAB010000011        59489 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPBAB010000011 DAPBAB010000000
VERSION     DAPBAB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 59489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.011"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="RD444_000874"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="RD444_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDT7548265.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="RD444_000875"
     CDS             850..1038
                     /locus_tag="RD444_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDT7548266.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="RD444_000876"
     CDS             1051..1608
                     /locus_tag="RD444_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7548267.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="RD444_000877"
     CDS             1640..1882
                     /locus_tag="RD444_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7548268.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2223..3437
                     /locus_tag="RD444_000878"
     CDS             2223..3437
                     /locus_tag="RD444_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDT7548269.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3468..4895
                     /locus_tag="RD444_000879"
     CDS             3468..4895
                     /locus_tag="RD444_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7548270.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4918..5844
                     /gene="glsA"
                     /locus_tag="RD444_000880"
     CDS             4918..5844
                     /gene="glsA"
                     /locus_tag="RD444_000880"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDT7548271.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6052..7512
                     /locus_tag="RD444_000881"
     CDS             6052..7512
                     /locus_tag="RD444_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548272.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7733..9496
                     /locus_tag="RD444_000882"
     CDS             7733..9496
                     /locus_tag="RD444_000882"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDT7548273.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9580..10038
                     /locus_tag="RD444_000883"
     CDS             9580..10038
                     /locus_tag="RD444_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDT7548274.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10147..10830
                     /locus_tag="RD444_000884"
     CDS             10147..10830
                     /locus_tag="RD444_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDT7548275.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10977..11591
                     /locus_tag="RD444_000885"
     CDS             10977..11591
                     /locus_tag="RD444_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDT7548276.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11607..11813
                     /locus_tag="RD444_000886"
     CDS             11607..11813
                     /locus_tag="RD444_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548277.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..13484
                     /locus_tag="RD444_000887"
     CDS             12354..13484
                     /locus_tag="RD444_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7548278.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            13576..14331
                     /locus_tag="RD444_000888"
     CDS             13576..14331
                     /locus_tag="RD444_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7548279.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            14389..16428
                     /locus_tag="RD444_000889"
     CDS             14389..16428
                     /locus_tag="RD444_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDT7548280.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(16537..17688)
                     /locus_tag="RD444_000890"
     CDS             complement(16537..17688)
                     /locus_tag="RD444_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDT7548281.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(17842..18372)
                     /locus_tag="RD444_000891"
     CDS             complement(17842..18372)
                     /locus_tag="RD444_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDT7548282.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(18471..18821)
                     /locus_tag="RD444_000892"
     CDS             complement(18471..18821)
                     /locus_tag="RD444_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDT7548283.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(18978..20507)
                     /gene="ahpF"
                     /locus_tag="RD444_000893"
     CDS             complement(18978..20507)
                     /gene="ahpF"
                     /locus_tag="RD444_000893"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDT7548284.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(20517..21080)
                     /gene="ahpC"
                     /locus_tag="RD444_000894"
     CDS             complement(20517..21080)
                     /gene="ahpC"
                     /locus_tag="RD444_000894"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDT7548285.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21224..21886)
                     /locus_tag="RD444_000895"
     CDS             complement(21224..21886)
                     /locus_tag="RD444_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7548286.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22070..23716
                     /locus_tag="RD444_000896"
     CDS             22070..23716
                     /locus_tag="RD444_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDT7548287.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            23707..24789
                     /gene="uxuA"
                     /locus_tag="RD444_000897"
     CDS             23707..24789
                     /gene="uxuA"
                     /locus_tag="RD444_000897"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDT7548288.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(24897..26450)
                     /locus_tag="RD444_000898"
     CDS             complement(24897..26450)
                     /locus_tag="RD444_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7548289.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            26598..27887
                     /locus_tag="RD444_000899"
     CDS             26598..27887
                     /locus_tag="RD444_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7548290.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(28013..29092)
                     /locus_tag="RD444_000900"
     CDS             complement(28013..29092)
                     /locus_tag="RD444_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7548291.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29201..29641)
                     /locus_tag="RD444_000901"
     CDS             complement(29201..29641)
                     /locus_tag="RD444_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7548292.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(29698..30108)
                     /locus_tag="RD444_000902"
     CDS             complement(29698..30108)
                     /locus_tag="RD444_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDT7548293.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30289..30723)
                     /locus_tag="RD444_000903"
     CDS             complement(30289..30723)
                     /locus_tag="RD444_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7548294.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(30823..31671)
                     /locus_tag="RD444_000904"
     CDS             complement(30823..31671)
                     /locus_tag="RD444_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7548295.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            32060..33826
                     /locus_tag="RD444_000905"
     CDS             32060..33826
                     /locus_tag="RD444_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDT7548296.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            33928..35187
                     /locus_tag="RD444_000906"
     CDS             33928..35187
                     /locus_tag="RD444_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7548297.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            35334..36635
                     /locus_tag="RD444_000907"
     CDS             35334..36635
                     /locus_tag="RD444_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7548298.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            36638..37489
                     /locus_tag="RD444_000908"
     CDS             36638..37489
                     /locus_tag="RD444_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7548299.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            37502..38317
                     /locus_tag="RD444_000909"
     CDS             37502..38317
                     /locus_tag="RD444_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDT7548300.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            38468..39634
                     /locus_tag="RD444_000910"
     CDS             38468..39634
                     /locus_tag="RD444_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7548301.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            39864..40511
                     /locus_tag="RD444_000911"
     CDS             39864..40511
                     /locus_tag="RD444_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDT7548302.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            40595..41539
                     /locus_tag="RD444_000912"
     CDS             40595..41539
                     /locus_tag="RD444_000912"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDT7548303.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            41674..43125
                     /locus_tag="RD444_000913"
     CDS             41674..43125
                     /locus_tag="RD444_000913"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDT7548304.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            43146..43982
                     /locus_tag="RD444_000914"
     CDS             43146..43982
                     /locus_tag="RD444_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDT7548305.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            43982..44641
                     /locus_tag="RD444_000915"
     CDS             43982..44641
                     /locus_tag="RD444_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7548306.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            44767..45456
                     /locus_tag="RD444_000916"
     CDS             44767..45456
                     /locus_tag="RD444_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548307.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            45462..46418
                     /locus_tag="RD444_000917"
     CDS             45462..46418
                     /locus_tag="RD444_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDT7548308.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            46462..46974
                     /locus_tag="RD444_000918"
     CDS             46462..46974
                     /locus_tag="RD444_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7548309.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(47230..47778)
                     /locus_tag="RD444_000919"
     CDS             complement(47230..47778)
                     /locus_tag="RD444_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7548310.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(47867..49048)
                     /locus_tag="RD444_000920"
     CDS             complement(47867..49048)
                     /locus_tag="RD444_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDT7548311.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(49284..50792)
                     /locus_tag="RD444_000921"
     CDS             complement(49284..50792)
                     /locus_tag="RD444_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDT7548312.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            50951..51142
                     /locus_tag="RD444_000922"
     CDS             50951..51142
                     /locus_tag="RD444_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548313.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            51345..52262
                     /locus_tag="RD444_000923"
     CDS             51345..52262
                     /locus_tag="RD444_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDT7548314.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            52255..53328
                     /locus_tag="RD444_000924"
     CDS             52255..53328
                     /locus_tag="RD444_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548315.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            53476..53994
                     /locus_tag="RD444_000925"
     CDS             53476..53994
                     /locus_tag="RD444_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548316.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            53975..54367
                     /locus_tag="RD444_000926"
     CDS             53975..54367
                     /locus_tag="RD444_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDT7548317.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            54443..55378
                     /locus_tag="RD444_000927"
     CDS             54443..55378
                     /locus_tag="RD444_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDT7548318.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            55511..56290
                     /locus_tag="RD444_000928"
     CDS             55511..56290
                     /locus_tag="RD444_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7548319.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFAMTAWTGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            56283..56600
                     /locus_tag="RD444_000929"
     CDS             56283..56600
                     /locus_tag="RD444_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7548320.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            56844..58454
                     /locus_tag="RD444_000930"
     CDS             56844..58454
                     /locus_tag="RD444_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7548321.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            58611..59459
                     /locus_tag="RD444_000931"
     CDS             58611..59459
                     /locus_tag="RD444_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7548322.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19738 a        10498 c        11636 g        17617 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat atgaagtatt
     1981 ctttattcct tatgaaaaaa tatctttaaa aataaaaaaa gacgaaaata aacggttttc
     2041 tataaaaaga agaccgttta ttttttgtct ttatttgtgt gaaaaacgaa aaagagcaca
     2101 acacatcaag cttttattaa ttgtctgtct tttataaatg caagtattaa ttcaaaaaaa
     2161 cagaagaaaa aaacaactac accttctttt atactcgtaa tcgtagaaag gaggcgaaaa
     2221 aaatgaatac attacctatc aatacaggag atacagcatt catgatttta tgtacagcaa
     2281 tggtttgcct gatgactcct ggtctagctt ttttttacgg cggattagca cggaagaata
     2341 atatactaac gatcatgtct caatcttttc tctccgttgg gataacaacg attatgtggg
     2401 tatttggtgg attcggattg gcctttggac gggatatcgg cggtgttatt ggaaacccgg
     2461 cagatttctt tttgatgaga catgttacaa gcttccccaa catgtttcat ggtgcaacga
     2521 ttccattttt aatgttcttt ctttaccaac tgatgtttat cgtgattacc gttcctttga
     2581 tgacaggagc ttttgcaggg agattgaatt taaaaggcta tattatactt gttatttttt
     2641 ggaatctgct gatttatttc cctgtttgcc actggatatg gggtggcgga tttttggatc
     2701 agatgggatt tagagatttc gctggtggag cagtcatcca cacgacagct ggattcggct
     2761 ctttagcatg tatcctcaca cttggtcaaa gaaagattcc tttaggagaa agaagtcagc
     2821 atagcagtct tatggcagct gctatcggta caggactgct ttggtttggc tggttcggtt
     2881 ttaattccgg tggtgcactt cgagcagatg aacaagcagt caatgcattt ggttcaacat
     2941 tcatcgctct tgcatttgcg atgattactt ggctgatcat tgcaaaagta aaaggaaatg
     3001 gctttgattt tgtcgacgta ctaacaggat ccgttgctgg attggcagcc attacgcctt
     3061 gtgccggtta tgtagaagca aaaagtgcca tgctgattgg gatcattgcg ggtatcgtct
     3121 gtcatgctgc tgttgatttt cgtaaaaaga aacaatggga tgatgcactt gatgtgtggg
     3181 gtgttcatgg catgggcgga ttcacaggta cgattctgat agggatcttt gcatctaaca
     3241 gccatttact aacgaatcca agcagctggt acttcttagg gattcaagtg attggtgtca
     3301 tcattactgc cgtgtatgcc tatgtgctga caacagtgat tttaaaatcg gcgatgcact
     3361 ttacaacgat cactactaca aaagaagaac aagaagaagg attggacttt ttcttctttg
     3421 gcaacaaact caattgaaac aaattcaaat agaaaagagg aagattcatg caacaaagca
     3481 aaacgaaaat tcctgcaaaa aaattaacgt tctttggctt tttggctatg accatctcaa
     3541 tggtcgtttc attatatgaa tatcccacat tcgcgacctc aggcttcagc ttagtatttt
     3601 tcttgttatt aggcggtttg ctatggttta tccctgtggc attgtgcgca gcagagatgg
     3661 caacggtgaa aggatgggaa aaaggcggtg tctatacttg ggtcagtcgc acattgggaa
     3721 aacgattcgg ttttgccgca atctttttcc aatggtttga gataacagtt ggctacttga
     3781 ccatgctgta tttcttaact ggtgcattat cctatgcaac gggtattggt gctatacaaa
     3841 acaataaata tctgaaattg gcaatcctat tgattatttt ctgggctatt ttactctcac
     3901 agttaaaagg aaccaaatac acttcattga ttgctcggat cggttttgta gctggtatct
     3961 tgttgccagc attagtcttg tttggcttag ggatccatta tgtcgcttca ggcgctccac
     4021 tacaaacgat actgtctatg aaaacattga ttcctgattt ttcgaaattg cctacgctgg
     4081 ttgtttttgt ttcctttatt ttagcatata tgggcgtaga aacatctgcc agtcatgcaa
     4141 atgaaatgga aaaccctaaa aaggattatc cattagcaat gtttgtattg gttattctgg
     4201 caatcatttt agatactttt ggcggattga ccgtcgctac tacagtacct caacatggtt
     4261 tgtctcttaa tacaggggtg attcaatcgt tagaattcct attaagacac attgatccaa
     4321 atcttgcatg gattgcgaaa attcttgcga ttcttgtttg tttaggtgtc atcggagaaa
     4381 ttgcttcttg ggtaacctct ccttcaaaag cacttcacgt agcagcagaa gaaggattac
     4441 ttcctgaata ttttgcaaaa gaaaacacac acggtgttcc tgctcatttg atgatcgcaa
     4501 atgggatcgt tgcaacaatc tgggcagccg tctttacatt aagcggcggg gggaataatc
     4561 tttcatttct tgcagcgatg tcattgactg ttgtaatcta cctgatgttg tactttatgt
     4621 ttttcgcggg ctatttgaag ctggttttta aagatgacag tcttgaaaga gcctatcagg
     4681 ttccaggcgg aaaagttgta aaagtaatcg ttgcttgttt aggtcttttg acttcacttt
     4741 ttgctttttg tatctcattt gttccacctg ctagtatcgc agctggacag catgggattt
     4801 atgaaacgat tctgattgtt tcttatctta tcgtcttagt tattccattt acgatctacg
     4861 catgccgtca taaattccag catgcagaaa aataaaaaat gaaagctggt tattcaaatg
     4921 gaagtaaaag aattagaaca attgattgga aaaaatttcc actattatca aacaggacaa
     4981 gtggcggatt acattccagc attagctgaa gtggatccca aacaattagg tatggcgatt
     5041 tatgatttgg atacaaaaca actgattgag ggaggagacg ccgatgtgcg ctttgccatc
     5101 gaaagcatgt caaaagttcc cgtattgtta ttagcaatca aagacaacgg aatagaaaaa
     5161 gtctttcaaa caatcaacac ggaaccaacc ggttttgcct tcaattctat catgaatatg
     5221 gaaatcaatc atcgcaaaca tccaatgaat ccttttgtta atgcaggagc gattgcaaca
     5281 acctcgttga ttgccggtaa agatgcagat gaaaaatttg agcgaatctt gtcatttatg
     5341 aaagagatct gtgatgatcc taatatcaca ttgaatgaag aaatctatca ttctgaatca
     5401 agaacaggtg atatcaatcg ctctcttgct tactatatga aaggtaatca aatgatcgaa
     5461 ggagacgtac cggagatact cgatgcttat ttcagacaat gctccctcaa tgtcaccgct
     5521 acaggcatag caaaattagc tgctgtgctg gcaaataaag gaattgctcc ttggaatggc
     5581 aaacaaatca ttactgaaga aagtgctaca atcgtcaaat caattatgac aactgctggt
     5641 ttgtatgatg aatcaggaga gttttccgtc catgttggtg tacctgcaaa aagcggtgta
     5701 ggcggcgggt tgatggcagc tgtgccaaat cgttacggca taggtgtctt cagcccggca
     5761 ctagatccat ttggtaacag cgcagctggt atccaattgt tgaaagatgt agtcaaagaa
     5821 ctagacgctg acatctttga ataaccattc ttgaaagagg ctggggtaaa agtccaaacg
     5881 gctcaaagct acctacaatg aggctgaaat tgtgagcttg tttgactttt gtcccagttt
     5941 ctttttttat tggtttttga aagggctttt ctaaataaaa aggaaacaaa aatacgattt
     6001 acagatatga ttaaataaaa tcataagaaa aactggaggc gtgtatcagg aatgctggaa
     6061 aattacatcg agcgaaatat atttagaaaa gtgtatctgt gtgaacaatt atttgaattc
     6121 caagaaatag acatcgaaca aacagctatt tctctaaggg tgactacacc aacgatcctt
     6181 cacgatctag aaagtctagc cgagtgtcta gaatattgta taaaagaaca agtaagagag
     6241 aagcataagt ataaactcgt attcaaacat ggtattgctc tttctgagct cacacagttt
     6301 ctgtacggtc aatcttattt tttaaagttt ttaagttatt attttaatgg tatattcacg
     6361 agtacagaac tggctgatct tgaatttatt tctttgagca aagtatatac aatcaaaaaa
     6421 atcgtattgg atttttttaa agaaaatgat tatttgaaaa ataaagaaat cattatccca
     6481 gaatttgact cgcggaacat cttgctagct ttagtacgtt atgtcaattg ggaaggctat
     6541 gaaaataaaa atccgtatgt agaagaagct tgtaacgagt tgattgatta tgtagagact
     6601 cattttttta aaagaagata ttcggaagat gaaaagtcct ttatcatacg aggaatagag
     6661 atcgcaatcg gaagaaaggc atttcccctt ggactgaaag aagaagataa gaaaagcgca
     6721 gaaacgaagc cgttatttca attagtctgt caaggattgg aagagcaaaa aggaattcta
     6781 gacttacaag aagaagatgt ctattatatt ttttctttgt tcaacagtag aaattatacc
     6841 aatgagaaca tggagctgtt aagaaaagat ttcgaagttg tttataaaaa ttttgttctc
     6901 aatgacctat cactgaatga attgatttta cagattatta gtatcgctga aatcgacgat
     6961 gcagaaaatt ttattttcaa gcaatccttt ttacaatttg tccgtacgtt gtgggcagat
     7021 ggacaagtat ttttaccgga aaggatctat cttctatcag aaaaagaaca acttctgtac
     7081 ggaaaaattg tagaaatatt ggaagagtgg aaaaagaaaa atcaaatcca actaagatgg
     7141 aatgaaaatt tcattcgaaa attcgtcaaa agtctttctt tgatcaacag tgaacaagcg
     7201 gggagacagg aaatagaaat ttttgttgtt tcagaaagtt cagtcaaaca atttttttat
     7261 cgaacccaat ttcatttgta tgcggaagaa ggcatcgctg aaataaatcc tattttattt
     7321 cgttcattag aagaattgcc tgatgaatgt ctatgtgcga aaaaaagagt ggttttgtgc
     7381 gatgctgcta gctatcaaaa tggattagaa actgaaaaga caaagatcta tccaatctca
     7441 ttaaaaggtg caaacatcca tttgtatcat attgctcagg aacttcacct attaaaaaaa
     7501 catatttctt gatgagacct taattaggag accttctaca aagcttttta attgtctgta
     7561 gaaggttttt atttgagtcc tgattcgtgc gaagatctac ttatataaca agctttctct
     7621 gaaatttttt taaaatgcta ttgacttaga gtcaactcta agtagtagaa tagataaagt
     7681 gtaacaaatc tattttcatt aaaagaagat agtacggagg agagaatgta ttatgagtac
     7741 agtattacat gtattggatg caatcaatag ccccgcagat gtcaaaaaat tatctgttga
     7801 ggaaatgaaa cagttggcag aagagatgcg ccaaaaaatc ttgcaaaagg acagcgatgt
     7861 aggtgggcac gttggtccga atctaggtgt agtagaatta acgattgcgt ttcattatgt
     7921 atttgattca ccaaaggata aaatcgtttg ggatgtttcc catcagtctt atggtcataa
     7981 gcttttaact ggtcgaaaaa atggttttga aaaaggacat taccgtgaag tgagcggata
     8041 ttccaaccaa catgaaagta aacatgacta ttttacagtg gggcacacat ctacatctat
     8101 cagcttagca acaggtttag ctaaagcgag agatctgaag aaagaatcag gaaatgtggt
     8161 agcatttatc ggtgacggct ccctaagtgg tggcttagct tttgaaggat tgaacaatgg
     8221 tggtgtttta caatccaatc taatcgtatt agtaaacgat aatgagatgt ctattgatga
     8281 gaatcatggc agtttatata aaaacttagc tgagcttcgt caaaccaacg gtaaaagcag
     8341 caataaccta tttaaagcaa tgggatttga ttatcgttac gtggataatg gaaacgatct
     8401 ggaaacggtg atccgtttat ttgaagaagt aaaagatatt gatcatccga ttgtacttca
     8461 cgtccatacg gaaaaaggtc gcggttatca gccggcaatc gataataaaa tgaaatatca
     8521 ttggcatgta ccatttgatc tgtccaccgg ggaggcaaag caagtaactt ctggagaaac
     8581 atttaatatg atcatcatgg attatttgga taagaaaatg caaacaagta cagctcctat
     8641 tgccgcagtc aatgcagcga taccaggcgc ctttggactg aaaacattcc aagaaaaaca
     8701 tccagatcat tattttgacg tggggatcgc tgaacagcac tctatcactt tcgcttctgc
     8761 tttggcttct cagggagtac gtccagtcgt cttccaatca agtactttct tgcaacgggc
     8821 atatgatcaa ttatcacatg atttagcgat caacgagaat ccagtgatta ttttagtcgg
     8881 tggcggaacg atctcagcta gtgaccgaac acaccaagga acatttgaca ttccttactt
     8941 atcaagtatc ccgaacatca aatatcttgc tccagcaaca aaagaggata tgatctctat
     9001 gctagattgg gcgttcgaac aaacagcgca gcctgtagca atccgaattc ctacacatgg
     9061 tgtagaacat ggtacagcta tcgcaaatga ttatagtatt ccagcctatc aaacagtaaa
     9121 taatggagga aaagtagcaa ttttagcttt aggcggtttt cttgaattag gaaaagaaac
     9181 agtaaaagct tttagaaaaa aaggaatcga agcaacgttg atcaatccag gaacgatcac
     9241 agaaaatgac gtagaaacat tagataaatt aaaagaaaca cacgaattag tcatcacatt
     9301 agaagatggt agtttgtcag gaggatttgg cgaaaatatc agtcgttatt atggtgattc
     9361 ttcaatgaac gtattaaatt ttggtgcaga gaaagaattc acggatagcg ttcctgtagg
     9421 gcagctttat gaacgttatc atttaacacc aaaacaaatc gttgaagata gtctgcgtgt
     9481 cttgaatatt caataattca ttatgctcac catccagata attgtttata ctagtatctg
     9541 gatggttttt tcattataaa aagcaaaggg gtggatgtta tgtatcagca agtagaagaa
     9601 tatgtcaaaa agttccaaga aaaatatcct agttatcaat cagaaaaatt aggtttcttt
     9661 tcatttgggg atacagaaaa aatggcagac gagttgagcg aactagttgt aaaaaagata
     9721 aagactggta catcaagtgg ttacgatctg tataaacaag agaatgagcc tttaccaaaa
     9781 gttggtcaat tagacgtggt cctgaatgga aaaaatgaac cagtttgtat catcaaaaat
     9841 ataaatgtga ccatcttgcc ttttagtgaa gtcacgaaag agcatgcttg gaaagaagga
     9901 gaaggggatc ggacattagc ttattggcgg aaagtccatg ctgagttttt taaacatgct
     9961 tataaagaag cgttgaacat agaatttaca gaagagaagc tggtcgtata cgaagaattt
    10021 gaagtaattt tcttttagta agaaaggtct gctgaaagaa atagctcatt attgtctcct
    10081 gaatagaaaa gggttacatt tttatatgaa gctgtatttt tatataacat tactggagga
    10141 gatattgtgg atacactgga acaaatcaaa ggcggagtcg tcgtttcttg tcaagctttg
    10201 gaaaacgagc cgctacacag ttcttttatt atggggagaa tggctttggc tgcaaaagaa
    10261 ggcggagcag ttggtattcg agcaaatacg acagaagata tcaatgaaat caagcgaatc
    10321 gttgatttac cggtaatagg gattatcaaa agagattacg atgggtcaga agtgtttatt
    10381 actgcgacag aaaaagaagt ggaagaactt ttggagacgc aagcagaaat cattgcactg
    10441 gatgcaactt ctcgtaaacg tccgacagaa aaaacaactg ctgaacttgt ccaaatgatt
    10501 cacgaacacg gtcggttagc tatggcagat atttcgactt ttgaggaagc gcttcaagcg
    10561 caagaagatg gttttgatct tttgtctacg actttagcag gctatacaga atattcacga
    10621 aagacgcaga cacctgattt tgatttattg aatcaaatca tagaacaagt cgatatccca
    10681 gtaattatgg aaggacatac tgactctcct gaacaagtag agaaagcata tgaattaggt
    10741 gctttttcag tcgtagttgg atctattatc acccgtccgc aattgatcac taaaagatat
    10801 gtcgaagccg caagtaaagc aaataactaa aaaacacgca cggcacagat tcttgacaaa
    10861 aaagaaaaat tgatagatag tttaaagcag attctaagaa gatgaaaaaa gcattacttt
    10921 tcattttgtc ttcaagatct gctttttaac gaattttata gaaagaaggc gaaaccatgt
    10981 ttgaacggag tccttcagaa ttgacagaac gtgaaaatta caagttgctg attggttcag
    11041 tcatccctag accagtagca gtagtaacga ctaaatcagc aaaagatata gtgaatatcg
    11101 cacccttcag ttacttcaat atcgtcagtt cgaatcgacc catcttatcc ttggcgatcc
    11161 agagaaaaca agatgcagta aaagacacag caaagaatat tcttgagaca agagaagctg
    11221 ttatccatat ccttgatgag gacaacgtag aagaagtaaa taaaacagca gccaatctgc
    11281 ctaatgagga aagcgaactg tacctgactg acttgacttt aactgattca gtaatcattt
    11341 cagtaccagc attgacagaa gcaaaagttc gttttgaaac atccttatat gaacatgtcg
    11401 agatacggaa taaaaataaa gtaacagctg atttattttt gctagaagta cagaaatatc
    11461 atttaaatga aacagtctat gatgataaga caggaaggat cgatccaaga aaattgaatg
    11521 ctgtaagccg tttggcagga aatgattatg cagcaatagg agaaattttc acgattgcac
    11581 gtcccgaata aaaaggagca ctagttatgt taaaaataag ctataaacca tcgacagatt
    11641 caaaagaaat gaaaaaagaa tatgaaacag tgaatgattt tctgcaaggg cagtacttag
    11701 aagttccgcc attgcaagac cattttgtcg ttactactgt tactttagat ggtaaggaaa
    11761 tagaaatgcc agatcaaacg attagcggat tgtttaatta ctttaacaaa taaatattta
    11821 ttccgaaaag tgaaaaaaca tataaaaaaa cacataaaac taaaaaatta tcaaagaatc
    11881 ctggtgcaac tgaaagaatt tgcaacggga ttctttcaac ttatttgtta tttttttcta
    11941 aaaaatatta aagattgtta aatttcacta tttcatttca aattccattg cctaatcatt
    12001 aggaaaagtc tatcatgaac tcgtaaatag aatatagtaa taaccttgtt aggtgaggct
    12061 cctatacaaa cataggctgt tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa
    12121 actgttggat taaggctttt tccaatagct aagaatgttt tctttacgtt gtatagtgct
    12181 aaaactcgga cgatgagatg atgctaggtc agaattcttg ttcgaaaagg tttctgacct
    12241 tttctttata tttttgtgac tgacttattt gatgagacaa actcgctcct cacctgacaa
    12301 gtggattgct gtgtgaggag gagatgcggt gaaaaaagat cgattggtgg attttgggtc
    12361 cttatttgac tttgtccatg atcggtcttt tagaagtata cagtgccagt tcttatcgtt
    12421 tattgcaagc cgatgaaaat acaaaaagtc tattattgcg tcaactaatt ttcatatttt
    12481 tgagttggag cgtgatcttc ttagctcgtt cagtcaaact acactattta cttcacccta
    12541 aaatagcagg atacggttta gccttatcga ttttcttttt agtattagta agaataggga
    12601 tattcggtgt cactgtcaac ggcgcacaac gttggatctc tctgtttggc attcaattcc
    12661 agccttctga actggcaaat ctttttttga ttttttattt aagctggttt tttcgtgacg
    12721 gaaatagtag cccaaaagat ctaaaaaaac cattcctgat tacagtaggt ataacttttc
    12781 tgattttatt tcagccaaag attgctggag cattgatgat cctttcgatt gcgtgggtca
    12841 tattttgggc agcggcggtt ccatttaaaa aagggatcta tctaatcgtt actttttctg
    12901 cattgctgat tggagcagca ggcggggtat tatatttagg aaataaaggt tggcttccac
    12961 agatgtttaa tcatgcttat gaaaggatcg cgactttaag agattctttt atcgatagtc
    13021 atggtgcggg ctatcaaatg acccattcgt tttatgcact atataatggt ggcatatttg
    13081 ggcggggatt aggaaatagt atcacaaaaa aagggtatct tccagagaca gaaacagatt
    13141 ttattttttc gattattaca gaagagcttg ggctaatcgg tgcattgtgt gtgcttttct
    13201 tattgttctc attatgcatg agaatctttt gtttaagcag tcgctgtaaa aatcagcaag
    13261 ctggattgtt tcttttagga ttcggaactt tgctattcgt tcagacgata atgaacgtgg
    13321 gaagtatcgc ggggctgatg ccaatgactg gtgtgccatt gccatttgtt agctacggag
    13381 gaacaagtta tctgatcctt tctttaggaa tcggaatcac gttgaatatt tcttcaaaga
    13441 tacaggccga agagttggcg ctctatcgac ctgaaaaaca ataaagagaa actgagaaac
    13501 tctcagtttg aattatcata aagtacaatc ttttattttt caagcgagag gttcttttag
    13561 aaagaggaat tcgcaatgaa agtatctcaa aaaatcatat tatctatttt gttactgctt
    13621 acattagtag ttggctattt ttcgattgag tttatccatg gattttcgtc tgcaaaacag
    13681 agttcaactg tgaaaaaggt agagccgaaa agtatcccta ccacactaaa tgtggctttg
    13741 attggttcag atgctcgttc gaaagacgaa aacggtcgct cggattcact tctggttgca
    13801 caatacaacc agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc
    13861 gatattccag gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat
    13921 ttattaaatc aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc
    13981 acatttcaag attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat
    14041 gcagaaaaat ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat
    14101 ggtaatactt tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg
    14161 atcagaaggc agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca
    14221 atattcaagc tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca
    14281 gaaagtgtgc ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag
    14341 gtataaatag tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt
    14401 gacaagcacg gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca
    14461 gctgcgggtg gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct
    14521 ggagaaaaga cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct
    14581 gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta
    14641 gataaatacc agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta
    14701 aaagtagata aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc
    14761 tcattaggtg aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa
    14821 accacgatca actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta
    14881 agcctgacca cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta
    14941 gcaacaacag gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc
    15001 gaaaaaacag ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc
    15061 aatcaggcga tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt
    15121 gatggagcaa cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat
    15181 tatcctttgg gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa
    15241 gatattgata agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag
    15301 atggcttttg ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca
    15361 gacggtgtag agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa
    15421 ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca gtctaggagg aaaagctgga
    15481 tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc
    15541 tatgatccaa acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa
    15601 aatcctgaac atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt
    15661 aaaatgatta cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg
    15721 acgatcaacg ggcttaaatg gcaaaaagac agttcttggg gatcttatca agcaacgcgt
    15781 gtcagtagtg atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata
    15841 tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa
    15901 ttcatttttg gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat
    15961 gaagatagct ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt
    16021 ctaattactc ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt
    16081 gtctatccta aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa
    16141 acagcagtac aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca
    16201 gcacattctc tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc
    16261 aaagaaaaac aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcttgat
    16321 aaccaaggtt atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc
    16381 aaacgagcat cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt
    16441 taagtacagt aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt
    16501 tcacgatgtc tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt
    16561 aaaatatttg agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg
    16621 tgaatagtac tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc
    16681 catttctcca cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc
    16741 ggcaatctga gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa
    16801 tatagaaata aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact
    16861 gacaaaaaat actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc
    16921 ttctttaacc tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct
    16981 aagcccaaca agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat
    17041 aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt
    17101 gactaataag aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga
    17161 agcattgcta gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac
    17221 taaaatgtca tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac
    17281 gtttagttct tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc
    17341 aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac
    17401 aaccgggaag atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa
    17461 aagagataaa tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga
    17521 aaagtcatgg actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac
    17581 accaacaagt aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc
    17641 aatcgttgtt gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc
    17701 tttgtataaa atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata
    17761 tactcacaaa aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc
    17821 tattttaata actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat
    17881 aatctcctgg atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa
    17941 acttttgaat ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata
    18001 aacactccaa cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat
    18061 gataacagct aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata
    18121 gacgatcaga gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc
    18181 tgttttcctt ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata
    18241 ggcggtgatc agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat
    18301 cccagaaata atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat
    18361 cgtctcaaac atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac
    18421 caaattaaat gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg
    18481 aggatctttt ccattttttt gccttttgct agctcatcga tcattttatc taaataacga
    18541 atttctttca tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta
    18601 atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact
    18661 tgtttttgta gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt
    18721 tcatctactt cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa
    18781 acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt
    18841 tttcaaatta tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa
    18901 aaaaacaaaa aggattgtga caaaattctt gtcaccatcc ttttttccgc tctctgactt
    18961 tttacaatct tcatagctca agtgcgcatc agataatcga atgctcctaa agctgcagta
    19021 gctcctgaac ccattgaaac aatgatttgt ttgtatggac tatctgtaca atcaccagct
    19081 gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg tcactatttc atttcttgga
    19141 ttcatttcga tcgttccatc tagccaatcc gtattaggga gtaagccgac tagaatgaat
    19201 actcctgaaa cagctagtgt atgcatctcc atcgtacggc gatcgatata ggagagagat
    19261 tctacctgat tcgtaccgct gatttctttc gtcgctgcat gtttgataac agtgacattc
    19321 gcaagagaga ctagtttatc ctgtaagatt tgatctgctt ttaattctgg taagaattct
    19381 agaacatata catgttttgc taacccagcc aaatcgattg ctgcttcaac accagaatta
    19441 ccaccaccga taacagcaat ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa
    19501 taagcaattc ctttattttt aaactctttt tcgcctggta cattgataga acgccagtgc
    19561 gcacctgtcg cgatgatggc ggtttttgct ttaagtgata taccgttagc taagccaata
    19621 ttaataaaat gttgctcttt atttaaggaa acggcctgtt gattcatcat aatgtcgata
    19681 gggtaactac gcacatgact ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag
    19741 gtaccaatca tattttcaat ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg
    19801 acaagtcctg tacggattcc tttacgtgct gcatagatag ctgcactact agctgctggt
    19861 ccaccgccaa tcactaatac atcataaggt tctttttcaa acaactcttc ttcaatcaaa
    19921 gggccagtga ttttttcaac aatttgttcg atcgtcatgc gtccgctact aaattcttct
    19981 ccatttaaaa acacagttgg cactgccatg atttttttat catcgatttc tttttggaac
    20041 atcccacctt caatcatcgt atgagtaata tttggattta atacagctaa tatattcaat
    20101 gcctgtacaa cgtcggggca attatgacaa gtcaagctaa cataactgtc aaaatgcaat
    20161 gtttgtgtta tttttttgat tttaagactc aaagattcac tgattttcgg tgcccgtcca
    20221 ctaatttgta acatagccag taaaaaagag gaaaattcat gaccgagggg gataccagca
    20281 aaaacgattc cactttcttt gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa
    20341 tctctttttt cccaagagat tttaggagac atttctgcta tttccttcgt aaattcagct
    20401 agtttttgac tattttcact attatccaaa ctaacagaaa aaacaatagg agattcaatc
    20461 agtgctaaat attgttcgag attttgttta gtttctttgt ctaacatatc caccacctaa
    20521 atctttccaa caagatcaaa acttggtttc aatgtttcgc cactttcttt ccattttgcc
    20581 ggacaaactt cacctgggtg ggtacgtaca tattgagcag cgcgaatctt atcaatcagt
    20641 gcactagcgt cacgaccgat tccatctgca ttgatttcca tagcttgaac gattccgtct
    20701 ggatcaataa taaatgttcc tcgctgagct aatccttgct cttcatccaa aacatcaaaa
    20761 atacgagaaa tctgatgaga cggatcaccg atcatcacgt attcgatttt accgatagca
    20821 tctgatgtat catgccatgc tttgtgagta aagtgggtat cggtagagca agaaaagact
    20881 tcaacaccta aagatttcaa tgtactgtat tgttcttgta agtcttctaa ttcagtagga
    20941 caaacaaaag taaaatcagc tggatagaag caaagaatac tccagttccc cataagatct
    21001 ttttcactga cttctaaaaa ttctccttga tgataagcat gtgcattaaa ttcttcgatt
    21061 tttttcccaa tcaatgacat agtaaaacct ccaataataa ttatttattt ataattattg
    21121 taatttagtt ggagttgcaa agtcaatgga tattttgtta tttaataaag attactcaaa
    21181 aacgaaggtt aattataaaa atagaagagg tcgcaagaaa atgctactgc tggaaatact
    21241 cgggataacg ttgagtaaca gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg
    21301 tttgaaatcg agcttcttct ggttttttat caactaaagc cttgagtaaa tcataatgtt
    21361 gttccatgat cgtttcccat ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt
    21421 ctaaatgggt attatggtca tctaaccagt tccaaacttc ttttcttcct gcaatttcga
    21481 aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc gcgttcgtca tttattttga
    21541 ctgctttttc ttgttcttct aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt
    21601 tggaacaaca ttcaatcatg atttgctctt ccaactgttc tcggacaaag cgagcatttt
    21661 ctgcagaaac aagattaatt tttgaaacat acgtaccact ttgaggtatc gtatacacga
    21721 gttcttgctg acgtagttga atcaatgctt cgcgaatggg agtccggccg atagcaagat
    21781 gttcctccag tgccttttca gatattttcc ttccaggttc taagtcagca tagataattt
    21841 gtttacgaat cgcttgataa gcttgagatt gcaagctgct tttcatcttt attcctccaa
    21901 aatcatttct atgaacgtag tataacagaa gaggtctgaa aaacaatctg gtatgtcaca
    21961 ttaactagta aatttgtata aatagtaaaa ttttggttga catagcgatt tttattgcat
    22021 aaaattcact tgaatacaaa aactaatatg ttagtttagg aggggaaaaa tgattgattt
    22081 aaacaactgg aggaacttta tccctgaatt atctcaaaaa gaagtagaaa tacctcattt
    22141 tgaccgtgca tcattaagag aaaatggaca aaaaaatcct gtctggatcc atttcggtgg
    22201 cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa cgtttagcgg atctaggcga
    22261 attgtccact ggtgtcattg tttgtgaaac attcgatgaa gaaataattg aaagggctta
    22321 ccaagatttt gatgatttga ttcttgaagt ggtcatgcca agcagtggag agttcgtcaa
    22381 acgtatatta gctgcgacag ctgacagtct gtattgtcat ccagattgtt ctgaacactt
    22441 tgataagatg attcgacact ttgaaaatcc tagtttacaa ttagttactt ttgctattac
    22501 tgaaaaaggt tatcgattga aagatacaaa taataaatat ataggaccga ttgcagtcga
    22561 tataaaaaat ggtccaaaga agccatcaca tacaatgagt attcttgctg cgctacttta
    22621 taaacgtttt ttatctggag cttggccgat tgcacttgtc tctacagata atttttcaca
    22681 aaacggtagt caattacgta cttctttgct aacgatcgca aaagaatggc aaaaggaagg
    22741 ttatgtacaa aatacattta tccaatactt atcgaatgaa gaaattgttg cgtttccatg
    22801 gacgatgatt gatcgtatca ccccgaatcc ttccttgtca gttagcaaaa tgttggcaaa
    22861 agagggcttt tcaaatatgt cgattttcca aaccgcaaaa ggaaataata ttgctccatt
    22921 tgtcaataca gaggacatcc attatttaat catggaagat cattttccca atggacgtcc
    22981 gaaattcgaa aaagctggag tattcttgac tgatcgtgca acggtagata aagcagatac
    23041 gatgaaggtg acgacttgcc ttaatccttt gcatacgtct ttagctatta tgggttgttt
    23101 actggggtat aggagcatag ctgaggagat gaaagatcca gatttatcgc tgctagtttc
    23161 tgaaatcggg tacaatgaag gcctcccagt tgtggagtct ccaggtatca ttgatccaaa
    23221 gatctttctg gaagaagtaa ttactgtgcg cttttctaat ccaaatattc ctgatatgcc
    23281 tcagcgaatc gccacggata cctctcaaaa ggtggcgatt cgttatggtg agacgattaa
    23341 aaaatatatg gaaatagaag ttaaagaccc gcagacactt gtattcatac cattgactct
    23401 cgctgcttgg cttcgatacc ttattggcat tgatgatcaa ggaaaaccat tcatcccgag
    23461 tccagaccca ctattgacag actttcagca taagctttcc gatattcaac tgggtacgca
    23521 agatacatca catattcatg agaaaatggc tcctattttg tcaaataaag aaatttttgg
    23581 tgtagatctt taccaagcag agcttggcag aaaaatagaa gaaatgttca tcaagatggt
    23641 agcaggaccg aatgccgtac gaaaaacatt acaggattac ttacaagaac atggaggaaa
    23701 tttatcatgg aaatgacttt tagatggtac ggatcacagg aagaaaagat taccttagat
    23761 gaaatcagac agattcctgc ggtcaaaggg attgtaggca cattgatgga tataccagta
    23821 ggagaggtat ggcctaggga gaggatcaaa gaactaaaag aagaaattga gtctcgtggg
    23881 ttaacactaa aagtaattga aagcgtcaat gtgcatgatg ccattaaaat tggattacct
    23941 tcccgtgagc agtatatcga aaactataag cagacaatcc gaaacttggc agaatatgga
    24001 atagaagtca tctgctataa ttttatgcct gtatttgact gggtgaaatc tgaccttgac
    24061 taccgcttag aagacggctc atccacgtta gcttttatat ctgatgatat tccagatgat
    24121 ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg aatttgaatt gccgggatgg
    24181 gaacctgagc gcttagctca tatcaaatcc ttgcttgaag cctatgcatc agtagacgaa
    24241 gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa tcatcccaac atgtgaagag
    24301 gtaggggtca aaatggctgt acatccagat gatccgccat actcgatttt tggattacct
    24361 cgaatcgtaa agaatcgaga agatttagat tggttgtgcc gcatagtaga cagcccttat
    24421 aacggaatca ctctttgtac agggagtatt gcagaagatc cagaaaatga tgtatatggg
    24481 attttagcag aattttgtca aagagatcga atcccatttg cacatgttcg aaatattaaa
    24541 tttttagcta atcgagattt ctatgaggca ccgcataaat ccgaatacgg ttcattagac
    24601 atgtaccgca tactaaaaac aatgtatgac aacggattta acggatacat ccgtcctgat
    24661 catggaagaa tgatctggaa tgagacagga agaccaggat acggcttata tgatcgagca
    24721 ttaggtgctg tttatctaaa tggattatgg gaagcaatcg aaaaaggaaa caaaagtaaa
    24781 gataattagt gaaaaaaact gtaattttat tatgtaaata agaaggatgt tcaaattctt
    24841 gatacataaa tccggaggtt gttaataagc attcggattt atgtatctta tttggtctag
    24901 gaagtaactt gattttcttt tttcgattga tccatgagtg cttcttcttt taattcaaag
    24961 atatattctg agatcctcat aatcactttt ttccagtctt ctggtgtgat gattcctttt
    25021 aaaatataga tttttttgat atgagaaggc aattgtttca aagggcagtc gctgataacg
    25081 atgttgcagt tctccataaa atcattttgt attttgatcg attcatttga aaaaatttgt
    25141 tctatcttaa cgatcagatc tttactgata taaaaatcac gcgtatagtt cacaccaatc
    25201 acaattcgcg gctgtttttt gtagcgatca tacaaatgta ctaggtcttc aaccatccat
    25261 tgcacatttt ccttttctaa aataactggt aacaagtgtt gattttgttc tctgaaatcc
    25321 atataaaatt gttcaatggc ggtttggact tctttcaaca cgctatgttc ttgataaagc
    25381 atgaaagtag gaaggggcgt atttggttga ttgaagttaa gaaatccatt ataaagttta
    25441 tttcgtgcga atgccaatga gtaaagaatc ttttcttttt caggtataaa caaatcaaat
    25501 acagtacaaa aggactccac catttctctt gaataaagaa catgaggaac gtttgctttt
    25561 tttaaaagtt tgtattgctc caagcttgtt tcttccgatt cgattttcgt gatagaaagt
    25621 cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa ttggcagatt aggtcgcaaa
    25681 atatctacag agggatcaat aaaaaagtca atatattcat actctgtgaa ttctttttgt
    25741 acgtctttca cagaagttgc aggaaaattc tctttacata tttttaatag aaaatataat
    25801 ttatctatca taccgctatt tagtcggtca agtacttctt ttttaaaaaa agtattcatc
    25861 ataaaagcca ctacttgcgg atcttcatca taaaattctg tagcagtact tgaaaagatg
    25921 ttccaataaa tatctaatgc tgcatattga atttgtacct cttttccaga aaaagacttt
    25981 ctgccattat tagaaaagga gatagacaaa ccgtacaatg ctaaaaatgc attaatcttt
    26041 ttcattttcg tatacaaata agaggcacta tagtttgttt gctgacttaa actgataatc
    26101 gaatttgtct gacagctgaa caaagaagtc aagatctgat aactgactga ttcgttgcag
    26161 tatcttccaa atagacgata atagcagtaa gccggatcaa ttccttcaaa attcaacttt
    26221 aaaaaaggtt tttgcttatc aatatgcagt tctgttagat tgagacgctc aaagtcttgt
    26281 tctaattctt tgatgtatag taaaaaattt gattcagaca tatggaaatg atctgctaaa
    26341 tattgtgtag taataaagaa acgatgttca gataggaaaa gaaaaatatc taattttgta
    26401 ttgctttttt tcttcaagag aaacgtttta atcgcttctt gagtttgcat gaaagaggct
    26461 cctttttact taaattgtta aaacttgaaa atattactgt ttttttcata aatttagaca
    26521 aaaaaacagt aaacatatag gtagaatctc tactatctta tttcttgcat acaaaaaaat
    26581 caaggagtga gagagttatg agatattcaa ccatcttaat atcaacattt gcagccttaa
    26641 gcttatcgct tactataaat cagacagtcc acacagagga gatagaaaca gccaaatgga
    26701 cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca ggatgaattt tcatccttca
    26761 attcaggagt cgcaaaatta agaaaaggac aaacagcgac catcaaagca aaaattgact
    26821 attcaggaga cttagcacct atactacaat cagctgttac atttgagaat gttgatcccg
    26881 ctttgtcgat tggctatgac gagaacactt tgactatcca acacaagcaa gcgatattta
    26941 ccttcaccgt tacattaaat caggacttgt taaaaccagc gctttttact gtcaaggtat
    27001 cagatggact accgaacgat catcatcatt taacgccgta tagtcagcgg cagacaattg
    27061 agcaagattc agctatcgac tcaggaggag atcttgttga agaaccaaca gataaaccag
    27121 aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa cagaaaccgg
    27181 aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac aatgaatcaa
    27241 aacctgaaat aacccctgga gaaaaaccag atgtagaccc agaagaaaaa ccggatgtga
    27301 ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag
    27361 ataatgaaac tgaaaatcca gaaaaaccag aggtagcccc agaagaaaaa ccagatgtaa
    27421 ctccagaacc agatacggat tctggaaacc aaacagtacc ggaaacaaat ccagacacag
    27481 ataatgaaac tgaaaatcca gaaaaactag aggtagatcc agaagaaaaa ccagatgtga
    27541 ctccagaacc agatacggac gcaagagacc aaggaatacc agaaaaaata aacaaaaaaa
    27601 caattcaaga ggatggaaaa aaagagagta aaaaatcaaa tctagcaatt ctaaaaataa
    27661 atgaagagca gctgaataaa aaatcacgaa tatttgatag cgcccaatca gctgaaacct
    27721 taaaaagtag caaggataca acctttgcta gtcctgaaac gaaaaataaa cagctaccaa
    27781 aatccggaga gtcacaaaac aaagttatac tgtggagcgg aatcattcta ttaagtatag
    27841 caacaatgct ttctgcaaaa agatttaaac aaaacagatc tctctaacat ttgaataatg
    27901 tctgtgaacg catttttcaa ataaaaagaa atatccagaa aacgacccca aaataccaga
    27961 ttgaaatctg atatttgggg tcgttcatac tttatctttc ttcatattta ttttaaggga
    28021 tatctgtttc ttttggaaga tggttcggga tctttttcac tttcaataca tctaaaaaga
    28081 aggtaaagat cggaatgccg acaatcaagc cccaaacgcc aaagaaccgt tcgctaacaa
    28141 gtaagaccac aaatgtataa aaaatcggca gttctgtttt gctggacatc aacttcggat
    28201 tcagcacata agactcaatt agatggacaa tcaaaatcgt aatcaggata taaacaacat
    28261 ctcggatgcc tccttgggaa taagctatga aagatagagg aatacaagaa atgataacac
    28321 ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc taaactcagc agctgatgaa
    28381 agccaaatac agccaaggcg attgtagtga gcaacgtgtt taaaagagcg ataacaaatt
    28441 gagcttctaa taccaaacca aatgtattga caaacttatc ggcaaaatag taaatatctt
    28501 gaaagaacca agaaaattca ctttttaaaa acaatcttga gaatacaatt gtttttttct
    28561 tttcaatcat aaaaaagaaa cttaagatga atgacatagc aaacgccact gccatttttc
    28621 ctatatcctg taaatagcca agcgcaatcg aagcgccatt ttgaagctgt tcaaaaaaat
    28681 cgtttcgctc aatataagta tggatatatt gtaaaacagg atctgcatct tttggctgat
    28741 gctcgtaaaa atcaataacg gactggatca tatcaaatgt ttgatgaata agcatcggga
    28801 tatactttgt gaatgccaga tagataaggt aaacgatcag tgtataaatc atgatagcta
    28861 aaacacctgt agggattcgg acaaatcgct gaatgatttg aacaagatga atcatcaaat
    28921 aagtaaagat aaatgtgagc aaaatggttg taatcatact tctaactaag aaaagaacaa
    28981 aaacgacaaa taataaaaca gtgagacggc gtattttttc attcgccaaa aaacgctgat
    29041 aaacggtcaa atcgacaacc tccaaataac aaaaaataaa cacgttattc attatatgat
    29101 ggaatatgaa aataagaaag agaaagagca aaaagaaaaa ccgctaaatg acgttatctt
    29161 atgagttggt tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt
    29221 gatgaaaatt atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt
    29281 caagtggaag ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag
    29341 caccttgagg agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat
    29401 taatataatt tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac
    29461 acaaatcatt tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca
    29521 caagatactg ggtataggta agattcagcg gcttgaggag ggaagtgtag tgtctaatga
    29581 tttccttaga ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca
    29641 tttttccacc tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta
    29701 ttcactaact gctttcagag taacttctat atttccagct gtagcttttg aatacggaca
    29761 cactttatgc gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga
    29821 tccttcaatc tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg
    29881 tgcactgaca gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact
    29941 gttaaaacat gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac
    30001 gcgtttacca ggagctgcaa tattcaattc gaatgtacga tcgggattat gaacttctcc
    30061 agagcgtccg cctgtgttga tcattttcgt agaatacatt tttttcataa aattaccacc
    30121 tttaattata ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg
    30181 gattggcata agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa
    30241 acatatacaa aaaaatatca gagacaaaaa ctaatctcat cataaacatt acatatattt
    30301 ctcaaaaagt tgttttcttt cttcaaaact tacttcacga tggacctgat gaagcatcca
    30361 taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt
    30421 catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc
    30481 agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc
    30541 aagagacgtg ttttcatcca gcatatggaa tctcgtgcca tacaaggtaa aaatgacttc
    30601 attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg
    30661 atacagttct agtgctttca aactatcatt taatacaaaa tctatttccg ttcctttatg
    30721 cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagtt
    30781 aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata
    30841 agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc
    30901 gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa
    30961 aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc
    31021 aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac
    31081 agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat
    31141 agataaaata gcataaagaa actcttctat tccaaaaaca aacagattaa gagaaacaac
    31201 gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat
    31261 gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc
    31321 taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt
    31381 ttctggaact attgcaatga ttaaaggaaa aaacaagctt ccgaaaagta ctttaaaaaa
    31441 gggtttttta cctaaaaata gaagagctag taccaacata acgatattca acactaaaat
    31501 aactatagct cggtcaaatc ctaaaacagc ttccagtaat attcctagac cgctgacacc
    31561 tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag
    31621 ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca tttatccact
    31681 tccttaattc attaataata ctcttatctt attataactt acttttcacc ttaagttaac
    31741 tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaatata
    31801 cttctacgtt aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct
    31861 taagataaat tcaggacact aaatagtaca ggaagtagat taaaactttt aaaagtttta
    31921 agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat
    31981 aacatttttg aaagcgttgt cttttgtttc tctatcactt atagtgatga cgtcatatta
    32041 aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata
    32101 tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat
    32161 caaaaaagta tatgtgcttt gtggagatcc atataccata acaactgaaa aatggtatca
    32221 aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga
    32281 agtgacgagc gaaacaaggc gcttacagta tgcgttccat gttgttgggg aagataacac
    32341 ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc
    32401 aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg
    32461 ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc
    32521 aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttctt
    32581 cgggggagat ttgcaaggtg tgagggatca tttggattac ttgacggacc ttggcgtcaa
    32641 tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga
    32701 ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga
    32761 agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca
    32821 ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt
    32881 ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag aaactgctga
    32941 aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa
    33001 tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat
    33061 tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag
    33121 agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc
    33181 tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat tcacagattc
    33241 gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa
    33301 tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc
    33361 tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt
    33421 gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg
    33481 gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca
    33541 agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa
    33601 gattatttct gaaggttcta ctcagtggct aatagtatat gatcgtgaag ataagctcta
    33661 tttcacacgg gaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc
    33721 ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga
    33781 cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt
    33841 aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc
    33901 acgttgaata cacaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta
    33961 gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca
    34021 gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa
    34081 gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta
    34141 gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag
    34201 ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat
    34261 ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg
    34321 acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat
    34381 aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa
    34441 gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg
    34501 actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat
    34561 gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct
    34621 tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca
    34681 ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca
    34741 tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact
    34801 ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat
    34861 tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat
    34921 caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca
    34981 gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa
    35041 gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc
    35101 gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc
    35161 agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat
    35221 ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt
    35281 gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc
    35341 aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga
    35401 tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc
    35461 tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg
    35521 gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca
    35581 tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag
    35641 cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata
    35701 taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta
    35761 cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc
    35821 atattccacc agccagttta attgattggg taggggctaa aaactttttt agtatcttct
    35881 ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt
    35941 tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga
    36001 aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag
    36061 cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata
    36121 ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga
    36181 aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc
    36241 cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag
    36301 cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg
    36361 tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct
    36421 ccatgatcta tctgtttaac aatggtggtc ccggaagcgt aggcggagga gctggttcta
    36481 ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta
    36541 tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat
    36601 ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc
    36661 tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt
    36721 cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc
    36781 agcttttagt ttagatcttt caagtgactg gactttagga aactttactc ggctattcag
    36841 tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat
    36901 cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg
    36961 ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt
    37021 gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac
    37081 aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga
    37141 cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat
    37201 ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc
    37261 ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa
    37321 tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc
    37381 tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt
    37441 acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat
    37501 catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc
    37561 gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt
    37621 gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca
    37681 atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga
    37741 attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt
    37801 ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga
    37861 gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa
    37921 ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt
    37981 agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct
    38041 tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg
    38101 gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat
    38161 gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga
    38221 ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt
    38281 tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac
    38341 aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa
    38401 cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag
    38461 gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc
    38521 tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc
    38581 ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcttc
    38641 tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc
    38701 attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa
    38761 atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca
    38821 ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg
    38881 tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca
    38941 tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg
    39001 gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact
    39061 aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa
    39121 atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac
    39181 aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt
    39241 aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg
    39301 cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt
    39361 tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt
    39421 acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga
    39481 tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc
    39541 ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc
    39601 tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac
    39661 tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct
    39721 aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa agtttggttc
    39781 tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata gaatactatt
    39841 ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt ttatttgcag
    39901 ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg aatacgtctt
    39961 ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca agcacatctt
    40021 cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc aaagtgtctg
    40081 cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt actagtttgg
    40141 aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat gatcaaaaag
    40201 aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat ccagaaactc
    40261 tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat gttgataaat
    40321 tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa
    40381 cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa gtaaaagacg
    40441 gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg tcaactgaac
    40501 aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt catgatacac
    40561 tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga cctttgccaa
    40621 tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga ctacccagga
    40681 attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct gtacgatcaa
    40741 tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga gtatcaattc
    40801 tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg cgtatgaaaa
    40861 agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga gtgtccagga
    40921 aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca ctgaattaaa
    40981 tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg aggccactta
    41041 tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca aattgcctcc
    41101 ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt ttccattgac
    41161 ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag aacaagaagc
    41221 agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata tcaagccgac
    41281 tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc aaatcattgg
    41341 tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg gcgcaagcat
    41401 gttacagatt ggtacagagc ttcataaaga agggccggaa attttttcta gaatcataaa
    41461 agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat ttaaaggcaa
    41521 attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt ctttttttat
    41581 aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta gacgaacttg
    41641 tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga attagcaaaa
    41701 gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca gaaaaaaata
    41761 aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc
    41821 cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc atttccttta
    41881 aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg tttatttgaa
    41941 acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat aggtctagtt
    42001 ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga cccagtcatc
    42061 tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc aagtggagga
    42121 gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga tggcggtggt
    42181 tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc gcgagggacg
    42241 atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga tttcggcttg
    42301 acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat ccattccggt
    42361 gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa acggttgaag
    42421 atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc agtacttgct
    42481 gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga gattccttac
    42541 ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc agaaacagca
    42601 gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga agaacttgaa
    42661 cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac ttacatcgat
    42721 tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca agaatacgat
    42781 ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt
    42841 gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga aggtttagac
    42901 gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca gttggctaat
    42961 ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa catgcctcta
    43021 ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat cggctatcta
    43081 tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa acaaggagaa
    43141 gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt attttaagcg
    43201 cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta gaaaaaaaga
    43261 aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat aatgatcaaa
    43321 atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca
    43381 aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca aaaagagaat
    43441 cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa ttctcccttt
    43501 cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat gattcaggaa
    43561 tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat actagctata
    43621 tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc acgaatgatc
    43681 agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat tattatctgc
    43741 agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag aacgtttatt
    43801 tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg aaaaagaaaa
    43861 tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata tctgaaaaat
    43921 attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg gtgagtgagt
    43981 gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt tcgtactgca
    44041 aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa atttggtagg
    44101 gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat ttattcgttt
    44161 ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt tatatttcgg
    44221 gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga ttaccagcag
    44281 tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag gtcagtggga
    44341 cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg ttttgccgca
    44401 agcttttcga atctcagtac cggcactcag taacgtagct atggatctag tgaaaggtac
    44461 ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga tcataggcgg
    44521 tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt gggtcatttg
    44581 tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatccatc agtcaatctg
    44641 aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt cttgtatatt
    44701 ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg ttcggaggta
    44761 gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca atattgcatc
    44821 gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg aattgtagat
    44881 acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta tgagctgaaa
    44941 gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca ttataattat
    45001 tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac aggaataggt
    45061 gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg tacatttctg
    45121 attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt gacaaaccgc
    45181 ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg aggcgtaaaa
    45241 tattttccaa aagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa tcaagatcca
    45301 ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca ggcaatcaat
    45361 gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag gcaacaacag
    45421 cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat ccttatttaa
    45481 gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga ttgctctcca
    45541 ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa aatgtgatcg
    45601 aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa gtaggacaat
    45661 tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc tctgtcgtcc
    45721 ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct ccagcgtttc
    45781 ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag ttggcaggag
    45841 ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg cccgaaaaca
    45901 aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat gcagataaaa
    45961 cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat attggatttt
    46021 ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc cgagcgcttg
    46081 ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat tttgtctctg
    46141 acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga gataaaatga
    46201 aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg attgaattat
    46261 tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag tggtcactta
    46321 tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg cttaaatatt
    46381 attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta aatgaaaaag
    46441 gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga agtagaagaa
    46501 gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc tcctcaatgg
    46561 caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag aaaagaaagc
    46621 tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc aggcgtggat
    46681 cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta tttatcgatt
    46741 catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc gttcttagaa
    46801 catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga tacttatcca
    46861 gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca agggatgatt
    46921 gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa ttgactataa
    46981 aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca tatgatagcc
    47041 aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc gaaaatgctt
    47101 ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg aaactccctt
    47161 catcgagagg tcgtgacata gcttttgtca cgatctctcg gcttatccta cgagtattat
    47221 tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt ggaacgctcc
    47281 atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact gtgctaaatc
    47341 ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac gttttttcag
    47401 gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta ccgtcccgaa
    47461 atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat ttcctactgt
    47521 cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat attggtccca
    47581 ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa caaaataata
    47641 atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa tgatcttttg
    47701 ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag gaccgataaa
    47761 tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga aaaagctttg
    47821 gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtattttat accagcaagc
    47881 cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt ttcaatccgt
    47941 gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca gaaatgatcc
    48001 cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct gttgtacgct
    48061 cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca tttgtaacca
    48121 gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt ccgctgatga
    48181 aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg aatatcccgt
    48241 ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata gcagcaaccg
    48301 tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa aatgtctgtt
    48361 ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag ggatttacaa
    48421 ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc acatatttag
    48481 aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac atagccgata
    48541 cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt atggaaataa
    48601 aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc aatttgccca
    48661 tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga agaatcttga
    48721 tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat acggaaagaa
    48781 agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca ccttgcccag
    48841 caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct gtaccaaaag
    48901 ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca attagtggtt
    48961 tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat gtgacaaata
    49021 aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt ccgttttatt
    49081 ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact tttttagagt
    49141 gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt taaaaagtgt
    49201 aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata gtttcgtttt
    49261 caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg gtagacaggt
    49321 gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt attgaccatc
    49381 acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc aacttgagcg
    49441 attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat gcttagtgca
    49501 tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag aactaatggt
    49561 gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt taagataact
    49621 gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc atctggtaat
    49681 tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat cttatctgca
    49741 tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac ttcttccata
    49801 atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct
    49861 gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac ggtatcatcc
    49921 acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata aacagattct
    49981 ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac atcccgatta
    50041 tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat attgacatct
    50101 tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa cggaacattc
    50161 catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg gctttcatga
    50221 tacagttggt tcaagcaagt agcgagcttt ccacttcctg ggccaggagc tgtcactaca
    50281 acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc tgagacgatt
    50341 ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt
    50401 ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg agtgataacg
    50461 acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa gatatccata
    50521 tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc agcatagacg
    50581 caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt cgcatcttct
    50641 tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctaacttccc accaaattct
    50701 aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg ctcttcaata
    50761 tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt actaaagcat
    50821 tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga
    50881 aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt tttgataagg
    50941 aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact atatccgttc
    51001 gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat tgacgtttac
    51061 ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc atttcacaac
    51121 aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga ttctgtccta
    51181 tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt
    51241 ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg tactattgaa
    51301 gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa gagccagata
    51361 caaatgcaaa ttatgagcca gacgaactag aggagacgaa cgcggatcag gatacaggac
    51421 aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt tcccaattat
    51481 tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct gagatctacc
    51541 gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga
    51601 tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt ccttttatgg
    51661 aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact tattaccatg
    51721 atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg cctaagatcg
    51781 atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg
    51841 agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat gaacaaataa
    51901 aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg aatcgtaaaa
    51961 aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct aatgaaaaat
    52021 taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa gaagcattga
    52081 agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa
    52141 tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa ttcttgatga
    52201 attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc gagtatgaat
    52261 gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg
    52321 aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga aatccctatg
    52381 ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc cgacgaatac gaatactgca
    52441 gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg aaaagagcaa
    52501 gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc ggatttacgt
    52561 atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata tgatcgtttt
    52621 atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga atacttcacg
    52681 tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc agcttatgac
    52741 acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa cgaattagtc
    52801 ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat gagtctccta
    52861 aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct ttctttgatc
    52921 cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt caatcacaaa
    52981 ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca gacattacta
    53041 gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac gaatgaagta
    53101 ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga aaagatcaac
    53161 gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac aacaaacgaa
    53221 aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt cattgatttc
    53281 tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt ggataagttg
    53341 gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt tatccagaat
    53401 aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc tgatgaagga
    53461 gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt gtctgctgct
    53521 tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat gactgctatt
    53581 tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat gagtctttct
    53641 attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca atggatcagc
    53701 tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat
    53761 ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat aggtatgacc
    53821 gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct tcaaggggtg
    53881 tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt tctgccagtc
    53941 gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt atagtttgct
    54001 tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata cacaatcggc
    54061 ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa
    54121 tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa ggcgatgatt tactgagtat
    54181 tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc ttgaagggca
    54241 ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga aaagtgctac
    54301 tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca atttgtctaa
    54361 actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca gtgagttcag
    54421 ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat
    54481 tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct atgcaatcag
    54541 tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca gcaatcagat
    54601 tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc atcaatttgg
    54661 tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg
    54721 tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc atgaagtggg
    54781 taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag gaagttcgtt
    54841 acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca cgatgcgctt
    54901 tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact tgtgtgctgt
    54961 aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg ggattgctgc
    55021 atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg ccattttcct
    55081 agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg atttatcaaa
    55141 aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa aaacagttca
    55201 tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta acaaagagaa
    55261 tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc ttcctgacaa
    55321 gaagatgtat atatacattg aaacagataa atatgaccca aattattctc gtaaataaaa
    55381 cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt aaataaaacg
    55441 gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg
    55501 agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat
    55561 gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt
    55621 tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca
    55681 gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt
    55741 tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa
    55801 agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt
    55861 gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa
    55921 ttggttcgca atgactgcat ggaccggtag caatatgatc ggtaattttt tcggctcggt
    55981 gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt
    56041 tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg
    56101 ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc
    56161 ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag
    56221 agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca
    56281 ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc
    56341 cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact
    56401 ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta
    56461 ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag
    56521 gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct
    56581 ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt
    56641 tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt
    56701 cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat
    56761 tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg
    56821 aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa
    56881 cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta
    56941 tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc
    57001 cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag
    57061 tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga
    57121 tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt
    57181 tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt
    57241 tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa
    57301 aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg
    57361 ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg
    57421 tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata
    57481 tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt
    57541 tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt
    57601 ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc
    57661 ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt
    57721 ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa
    57781 gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag
    57841 taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt
    57901 tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt
    57961 tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc
    58021 ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga
    58081 aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa
    58141 atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc
    58201 gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga
    58261 ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat
    58321 caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt
    58381 tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca
    58441 gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta
    58501 tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg
    58561 atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg
    58621 cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat
    58681 tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg
    58741 aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac
    58801 ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga
    58861 ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga
    58921 aagatgacat cagcggagta acggtcattc cttatgactc taagatcact agtatgccga
    58981 tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa
    59041 tacttgcaca tatcgatcga atccgagata atacctatgc ttatctgatt gtagatgatt
    59101 tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat
    59161 taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc
    59221 gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa
    59281 tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg
    59341 agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac
    59401 cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat
    59461 tccttttatt caaaaaacat accaaaaat
//
LOCUS       DAPBAB010000012        56347 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPBAB010000012 DAPBAB010000000
VERSION     DAPBAB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.012"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            290..1135
                     /locus_tag="RD444_000932"
     CDS             290..1135
                     /locus_tag="RD444_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7548323.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="RD444_000933"
     CDS             1416..2636
                     /locus_tag="RD444_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDT7548324.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="RD444_000934"
     CDS             2633..5353
                     /locus_tag="RD444_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7548325.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="RD444_000935"
     CDS             5337..6281
                     /locus_tag="RD444_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7548326.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="RD444_000936"
     CDS             complement(6350..7360)
                     /locus_tag="RD444_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7548327.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="RD444_000937"
     CDS             complement(7540..7884)
                     /locus_tag="RD444_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7548328.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="RD444_000938"
     CDS             complement(7877..8311)
                     /locus_tag="RD444_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDT7548329.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="RD444_000939"
     CDS             8449..9186
                     /locus_tag="RD444_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7548330.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="RD444_000940"
     CDS             9179..10390
                     /locus_tag="RD444_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548331.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="RD444_000941"
     CDS             10594..11376
                     /locus_tag="RD444_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDT7548332.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="RD444_000942"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="RD444_000942"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDT7548333.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="RD444_000943"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="RD444_000943"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDT7548334.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="RD444_000944"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="RD444_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDT7548335.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="RD444_000945"
     CDS             14978..16027
                     /locus_tag="RD444_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDT7548336.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="RD444_000946"
     CDS             16024..16302
                     /locus_tag="RD444_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDT7548337.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="RD444_000947"
     CDS             16277..16747
                     /locus_tag="RD444_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDT7548338.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="RD444_000948"
     CDS             16764..17066
                     /locus_tag="RD444_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548339.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="RD444_000949"
     CDS             17083..17454
                     /locus_tag="RD444_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDT7548340.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="RD444_000950"
     CDS             17426..17785
                     /locus_tag="RD444_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548341.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="RD444_000951"
     CDS             17957..18964
                     /locus_tag="RD444_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7548342.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="RD444_000952"
     CDS             18989..20173
                     /locus_tag="RD444_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDT7548343.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="RD444_000953"
     CDS             complement(20610..22193)
                     /locus_tag="RD444_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7548344.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="RD444_000954"
     CDS             complement(22771..22914)
                     /locus_tag="RD444_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDT7548345.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="RD444_000955"
     CDS             complement(23137..23616)
                     /locus_tag="RD444_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7548346.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="RD444_000956"
     CDS             complement(23689..23973)
                     /locus_tag="RD444_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548347.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="RD444_000957"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RD444_000957"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="RD444_000958"
     CDS             complement(24317..25603)
                     /locus_tag="RD444_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDT7548348.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="RD444_000959"
     CDS             25741..26412
                     /locus_tag="RD444_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDT7548349.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="RD444_000960"
     CDS             26378..26866
                     /locus_tag="RD444_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDT7548350.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="RD444_000961"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="RD444_000961"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDT7548351.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="RD444_000962"
     CDS             27827..28591
                     /locus_tag="RD444_000962"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDT7548352.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="RD444_000963"
     CDS             28824..31037
                     /locus_tag="RD444_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDT7548353.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="RD444_000964"
     CDS             31048..31494
                     /locus_tag="RD444_000964"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDT7548354.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="RD444_000965"
     CDS             31665..32924
                     /locus_tag="RD444_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDT7548355.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="RD444_000966"
     CDS             complement(33013..33960)
                     /locus_tag="RD444_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7548356.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="RD444_000967"
     CDS             34354..35646
                     /locus_tag="RD444_000967"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDT7548357.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="RD444_000968"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="RD444_000968"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDT7548358.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTRAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="RD444_000969"
     CDS             37539..38414
                     /locus_tag="RD444_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7548359.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="RD444_000970"
     CDS             38680..40563
                     /locus_tag="RD444_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDT7548360.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="RD444_000971"
     CDS             complement(40638..40922)
                     /locus_tag="RD444_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDT7548361.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="RD444_000972"
     CDS             complement(40912..41646)
                     /locus_tag="RD444_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDT7548362.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="RD444_000973"
     CDS             41775..42398
                     /locus_tag="RD444_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDT7548363.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="RD444_000974"
     CDS             42590..43711
                     /locus_tag="RD444_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDT7548364.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="RD444_000975"
     CDS             43718..46846
                     /locus_tag="RD444_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDT7548365.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="RD444_000976"
     CDS             46865..50059
                     /locus_tag="RD444_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDT7548366.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="RD444_000977"
     CDS             complement(50205..50912)
                     /locus_tag="RD444_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDT7548367.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="RD444_000978"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="RD444_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDT7548368.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="RD444_000979"
     CDS             52407..53060
                     /locus_tag="RD444_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDT7548369.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="RD444_000980"
     CDS             53286..54284
                     /locus_tag="RD444_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDT7548370.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="RD444_000981"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="RD444_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDT7548371.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18537 a         9724 c        11680 g        16406 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca agagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 atttttt
//
LOCUS       DAPBAB010000013        56283 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPBAB010000013 DAPBAB010000000
VERSION     DAPBAB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 56283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.013"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(81..1637)
                     /gene="rny"
                     /locus_tag="RD444_000982"
     CDS             complement(81..1637)
                     /gene="rny"
                     /locus_tag="RD444_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDT7548372.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLLIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2048..3097)
                     /gene="recA"
                     /locus_tag="RD444_000983"
     CDS             complement(2048..3097)
                     /gene="recA"
                     /locus_tag="RD444_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDT7548373.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3206..4447)
                     /locus_tag="RD444_000984"
     CDS             complement(3206..4447)
                     /locus_tag="RD444_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDT7548374.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="RD444_000985"
     CDS             complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="RD444_000985"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDT7548375.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5474..6397)
                     /locus_tag="RD444_000986"
     CDS             complement(5474..6397)
                     /locus_tag="RD444_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548376.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7136..8422)
                     /locus_tag="RD444_000987"
     CDS             complement(7136..8422)
                     /locus_tag="RD444_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDT7548377.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8415..9683)
                     /locus_tag="RD444_000988"
     CDS             complement(8415..9683)
                     /locus_tag="RD444_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDT7548378.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9935..10396
                     /gene="mscL"
                     /locus_tag="RD444_000989"
     CDS             9935..10396
                     /gene="mscL"
                     /locus_tag="RD444_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDT7548379.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10455..10901)
                     /locus_tag="RD444_000990"
     CDS             complement(10455..10901)
                     /locus_tag="RD444_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7548380.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10994..11239)
                     /locus_tag="RD444_000991"
     CDS             complement(10994..11239)
                     /locus_tag="RD444_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDT7548381.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11354..11932)
                     /locus_tag="RD444_000992"
     CDS             complement(11354..11932)
                     /locus_tag="RD444_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7548382.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(12009..12539)
                     /locus_tag="RD444_000993"
     CDS             complement(12009..12539)
                     /locus_tag="RD444_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDT7548383.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="RD444_000994"
     CDS             complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="RD444_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDT7548384.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13396..13791)
                     /locus_tag="RD444_000995"
     CDS             complement(13396..13791)
                     /locus_tag="RD444_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDT7548385.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13788..15200)
                     /locus_tag="RD444_000996"
     CDS             complement(13788..15200)
                     /locus_tag="RD444_000996"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDT7548386.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="RD444_000997"
     CDS             complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="RD444_000997"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDT7548387.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15819..16058)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16055..17515)
                     /locus_tag="RD444_000998"
     CDS             complement(16055..17515)
                     /locus_tag="RD444_000998"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDT7548388.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17685..18842)
                     /locus_tag="RD444_000999"
     CDS             complement(17685..18842)
                     /locus_tag="RD444_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDT7548389.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18876..20246)
                     /gene="radA"
                     /locus_tag="RD444_001000"
     CDS             complement(18876..20246)
                     /gene="radA"
                     /locus_tag="RD444_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDT7548390.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20309..20836)
                     /locus_tag="RD444_001001"
     CDS             complement(20309..20836)
                     /locus_tag="RD444_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDT7548391.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            21066..21872
                     /gene="proB"
                     /locus_tag="RD444_001002"
     CDS             21066..21872
                     /gene="proB"
                     /locus_tag="RD444_001002"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDT7548392.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            21869..23116
                     /locus_tag="RD444_001003"
     CDS             21869..23116
                     /locus_tag="RD444_001003"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDT7548393.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            23192..23536
                     /locus_tag="RD444_001004"
     CDS             23192..23536
                     /locus_tag="RD444_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548394.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(23653..23979)
                     /locus_tag="RD444_001005"
     CDS             complement(23653..23979)
                     /locus_tag="RD444_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548395.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(24352..25553)
                     /locus_tag="RD444_001006"
                     /pseudo
     CDS             complement(24352..25553)
                     /locus_tag="RD444_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            25670..26185
                     /locus_tag="RD444_001007"
     CDS             25670..26185
                     /locus_tag="RD444_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7548396.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(26384..26830)
                     /locus_tag="RD444_001008"
     CDS             complement(26384..26830)
                     /locus_tag="RD444_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDT7548397.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="RD444_001009"
     CDS             complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="RD444_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDT7548398.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(28709..29338)
                     /locus_tag="RD444_001010"
     CDS             complement(28709..29338)
                     /locus_tag="RD444_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7548399.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(29322..29786)
                     /locus_tag="RD444_001011"
     CDS             complement(29322..29786)
                     /locus_tag="RD444_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7548400.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(30273..31541)
                     /locus_tag="RD444_001012"
     CDS             complement(30273..31541)
                     /locus_tag="RD444_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7548401.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(31772..32509)
                     /locus_tag="RD444_001013"
     CDS             complement(31772..32509)
                     /locus_tag="RD444_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7548402.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(32576..33145)
                     /locus_tag="RD444_001014"
     CDS             complement(32576..33145)
                     /locus_tag="RD444_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDT7548403.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            33273..34145
                     /locus_tag="RD444_001015"
     CDS             33273..34145
                     /locus_tag="RD444_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7548404.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            34487..35359
                     /locus_tag="RD444_001016"
     CDS             34487..35359
                     /locus_tag="RD444_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7548405.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            35356..37314
                     /locus_tag="RD444_001017"
     CDS             35356..37314
                     /locus_tag="RD444_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7548406.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(37565..38416)
                     /locus_tag="RD444_001018"
     CDS             complement(37565..38416)
                     /locus_tag="RD444_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7548407.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            38539..39006
                     /locus_tag="RD444_001019"
     CDS             38539..39006
                     /locus_tag="RD444_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDT7548408.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(39269..39751)
                     /locus_tag="RD444_001020"
     CDS             complement(39269..39751)
                     /locus_tag="RD444_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDT7548409.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(39851..40714)
                     /locus_tag="RD444_001021"
     CDS             complement(39851..40714)
                     /locus_tag="RD444_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7548410.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            41312..42160
                     /locus_tag="RD444_001022"
     CDS             41312..42160
                     /locus_tag="RD444_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548411.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(42331..42672)
                     /locus_tag="RD444_001023"
     CDS             complement(42331..42672)
                     /locus_tag="RD444_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDT7548412.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            42758..43465
                     /locus_tag="RD444_001024"
     CDS             42758..43465
                     /locus_tag="RD444_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7548413.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            43452..43775
                     /locus_tag="RD444_001025"
     CDS             43452..43775
                     /locus_tag="RD444_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7548414.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(43864..44901)
                     /locus_tag="RD444_001026"
     CDS             complement(43864..44901)
                     /locus_tag="RD444_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7548415.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(45348..46331)
                     /locus_tag="RD444_001027"
     CDS             complement(45348..46331)
                     /locus_tag="RD444_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDT7548416.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(46747..47517)
                     /locus_tag="RD444_001028"
     CDS             complement(46747..47517)
                     /locus_tag="RD444_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548417.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(47510..48445)
                     /locus_tag="RD444_001029"
     CDS             complement(47510..48445)
                     /locus_tag="RD444_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7548418.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(49039..50688)
                     /locus_tag="RD444_001030"
     CDS             complement(49039..50688)
                     /locus_tag="RD444_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDT7548419.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(50883..51779)
                     /locus_tag="RD444_001031"
     CDS             complement(50883..51779)
                     /locus_tag="RD444_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7548420.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(51772..52452)
                     /locus_tag="RD444_001032"
     CDS             complement(51772..52452)
                     /locus_tag="RD444_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7548421.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(52453..53235)
                     /locus_tag="RD444_001033"
     CDS             complement(52453..53235)
                     /locus_tag="RD444_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548422.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(53228..54145)
                     /locus_tag="RD444_001034"
     CDS             complement(53228..54145)
                     /locus_tag="RD444_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7548423.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(54148..54432)
                     /locus_tag="RD444_001035"
     CDS             complement(54148..54432)
                     /locus_tag="RD444_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548424.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(55133..55753)
                     /locus_tag="RD444_001036"
     CDS             complement(55133..55753)
                     /locus_tag="RD444_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548425.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(55834..>56283)
                     /locus_tag="RD444_001037"
     CDS             complement(55834..>56283)
                     /locus_tag="RD444_001037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548426.1"
                     /translation="NRSYGSPLFCECVVVTLFYRRDFLFSIYTKYFTLSSNIKTSTKL
                     ICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSE
                     AEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
BASE COUNT        16115 a        11976 c         9986 g        18206 t
ORIGIN      
        1 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
       61 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
      121 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
      181 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
      241 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
      301 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
      361 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
      421 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
      481 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
      541 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
      601 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
      661 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
      721 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
      781 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
      841 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
      901 tcatcgatga gtaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
      961 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
     1021 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
     1081 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
     1141 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
     1201 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
     1261 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
     1321 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
     1381 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
     1441 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
     1501 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
     1561 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
     1621 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
     1681 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
     1741 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
     1801 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
     1861 catataggta aacctttaaa ttgagaaact tttctattaa attattcttt gtagatgaac
     1921 agatattttt attttatgta cccactcgta taattaaata actcacgatt tctttattca
     1981 ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag accagccgtt tttgtttatc
     2041 ataattttta ttcttctact aaaggcaatt cttcttgtcc tttttcatct tctgggacag
     2101 cgacttcttc accaatacca taagctgctc taactagagc agaaacttct gccatcattt
     2161 caggatgatt agccatatag attttagcgt tttcccgtcc ttgaccaatc cgatcttctt
     2221 tataagaata ccaagcaccg cttttatcaa caatgtcttt ttctactgcc atgtctagaa
     2281 gttctccttc ttgtgagatc cctaaaccat acatgacatc tacttcagcg attttgaaag
     2341 gaggtgctac tttgtttttt acgactttga tctttgtacg gttaccaaca atatctgtac
     2401 cttgtttcaa ttgttctgca cgacgtactt ccaaacggat cgttgcgtag aatttcaatg
     2461 cgcgtcctcc aggagtgatt tctggattcc caaacatcac gcctactttt tcacggattt
     2521 ggttgatgaa aatagcgatt gtttttgttt tgttgatcga accagagagt ttacgcaatg
     2581 cttgagacat caaacgtgct tgtaacccga catgtgagtc acccatttcg ccgtcgattt
     2641 ctgctcgtgg aactaaagca gcaactgaat cgactactac gatatctaca gccccacttg
     2701 atactaaagc atcagcgatc tctagacctt gttctcctgt gtcaggctgt gaaagaagta
     2761 gttcatcgat attcacacct aatttttgtg catattgcgg atctaacgca tgctcagcat
     2821 caatgaaagc ggccgttccg ccattttttt gtacttctgc aatagcgtgt agtgcaactg
     2881 ttgttttacc tgaactttca ggtccatata cttcgatgat acgtccacgg gggtaaccgc
     2941 cgacacctaa agcgacatct aacgcaagag agccacttgg aattgtagag atttgttggt
     3001 cgattttttc gcccaatttc ataatagagc ctttaccata gctcttttca atttttttca
     3061 gtgcagcatc taaggctgct ttacgatcat ctgccaatac gataatcctc cttatattta
     3121 caaatcagag caacatttca ttatccttat catatctttt ttagtgaaaa aaagcaagaa
     3181 aaaaccgaac aaatattcgc atttatcatt ttcttaaaag ctgttttctc aaaagatcaa
     3241 atccttgcat aaccgcatta tatctgatag cagcacgatc tctggcaaag tgatattttt
     3301 ccgaaaccac gccgtcttta gatgaaatag cgatccatac tgttcctgca gattgacctt
     3361 ctaacgtgtc cggtccagcc actccggtga aagctacact gtaatctgtt cttgctatct
     3421 cacgtgcttg aatggccatc tgctccacac attctttgct gactgttcca tattctgcta
     3481 atagctccgg atcaatctcc agaaaaccag cttttgtttg gagcgagtag gtaacgaatc
     3541 cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg
     3601 ctgtcaaact ttccgctgct gttacagtct gcttgttttc tttcagtagt tccactacga
     3661 cttttgctag cgaattatca tcgccgtatc cataaaaata ttcccctact cgttcttgga
     3721 ttttttcttc taatgctaaa agagcttgat tccctgcctg aacatcattt gttttcactg
     3781 tcagtcgtaa tgtcacttca tttggtttgg catatggtgc gatcgttgga ttgatctgtg
     3841 tctcaatcag atcttttagc tcagtgacca gtcgcgattc accaatccca taaaagcgca
     3901 aaactttaga aattagtttt tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt
     3961 gttccacaaa cattggtttc aactcattag gcggaccggg aagaaggata tacgcatgtg
     4021 tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg atttggtaga ggaaccccgc
     4081 cttcgataat ctgggactgt tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa
     4141 aatatgccaa taattttttg tatccttccg tattttgtat gagagactta ccgaggtgag
     4201 ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc taatccgcca cataagagta
     4261 tgagttcact tcttgtttca gctagagcaa tcaactcttc taaacgctgc tgattatctc
     4321 ctacaactgt ttgataataa acttcgatac ctagatcagc tagctgttct gacaagaaag
     4381 tggcattcgt attcacgact tgtcctagta aaagttctgt tccgacagct attatttctg
     4441 ctttcattac ttcccgctcc cttatatagt atacgctgta tttttatatt tttttattta
     4501 aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc tccttgttcc aaataaacag
     4561 tgttctcctg ccaaaacctc ggtcataagt cacaatatac ataaaacatg aaaagctgtt
     4621 ttttgaaatt atcccttgtg actcagagtt agacggcagg ctcacaacct ctccactaaa
     4681 cgatacttac attgatcctt tgaacacacc gctgtttttg atgaagtaat ccacacctga
     4741 atagatagta aagatcagac aagcataaag catgatttga tctactggaa taccgatcag
     4801 gctgaatggg atattattga taaacagcaa gataatagca accatttgtg tagccgtctt
     4861 gacttttccc ggccaagctg cagccatgac ttctccatgt tcgacaagta gtaaacgcaa
     4921 gcctgtgacg gctaattcac gacagacaat gatcgctact acccaaccag gggcctttcc
     4981 ttggtcaacc aacatgataa aagcagtcat aactagcatt ttatctgcca acgggtccgc
     5041 aaacttgcca aaattagtga caaggcctcg cgcacgggca atttttccat ctaaccagtc
     5101 tgtgatacta gctaaagcaa aaataactgc acctactagc tgtgtgacag ccagtgtggt
     5161 gtctcctacg ttaaattgtc cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg
     5221 gatcatacaa attctaagta cagttaattt atttggtaaa ttcacactat cgactccttt
     5281 cttccctatt ctacgtgact atcagcgaag tttccaccga tattatttac ttttccaaat
     5341 gaatttattc aaaggagtat ttttattgaa attgatcttt ttgaattaac gaagaaggac
     5401 cgcgacacag atttgtcacg atccttcttt tttataccta caagtaaaca gatgcgtaaa
     5461 ctaatttata cgatcattct gcgtattgaa gagtaaggtt cacatttttt ggactctgat
     5521 tgttttcccc tggattaact ggtacggatt ctccattcgc tttgatcgtt gcattgcttg
     5581 ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg taacacggta ctctgtgttt
     5641 catttgctgc cagtgtccct tgataaacat aggcattgtc tactagcaca cctaaccata
     5701 cacgattgac agcagtaaat tcaagggtta caggcttggt agcgtctgtg atatttactg
     5761 cgatggcact gccagaatct tctcctgtag ctattgccat tttcttttct tcacttgatt
     5821 ttgttgttga ttctactgta gaagactctt tggtctgcgt gctttgctct gaggaagagg
     5881 actcctcgac tgatccttct actttaagcg aagaagctgt gtctccgatt attggatcag
     5941 cttgacgatc ttgccaagtc atatagccca cgacaacgac aatcgccaaa gcaaccaatc
     6001 ctaataaaat gacaggaaga cttgtccaaa agcgacttgg atgcgattct tcctgatgca
     6061 ttgcttttct agaagtactt actgtttcag gctgcggacg ttcagggacc gatcctgtca
     6121 atttgctttt cccatcaaaa acatcgacta aatggtctcc atcttctcct acagcttcag
     6181 cgtattgccg aataaatgcc cgcacgtaaa atttaccagg caattggtca aaatcatcat
     6241 tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag
     6301 atattttttt attcaggcgt gcttgccgta atttttctcc gatggtttca aaagccactc
     6361 gttcatcttc tccagtctgt ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt
     6421 atgtttgggt gctgtgacaa aagtcatgtc acagctcctt ttttccgaat aaacattgtt
     6481 ctcctgccaa ctcctcggtc acaagtcaca atatccaata aatataagaa gctgtttttt
     6541 gaacttgttc cttgtgactc ggagttaaac ggcaggctca cgacctcttt cttaaacggt
     6601 gttcaatcag ctgttcctcg gtattagctc aaaaaccggc aaaatataag gagatatttt
     6661 tccgtatgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
     6721 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct
     6781 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttggta
     6841 cacggtgttc cagaagtagc ttcttcggca ataagccgaa atatcacaaa aatttgagaa
     6901 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcttt
     6961 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
     7021 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
     7081 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
     7141 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
     7201 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
     7261 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
     7321 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
     7381 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
     7441 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
     7501 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
     7561 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
     7621 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
     7681 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
     7741 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
     7801 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
     7861 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
     7921 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     7981 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     8041 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     8101 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     8161 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     8221 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     8281 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     8341 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     8401 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     8461 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     8521 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     8581 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     8641 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     8701 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     8761 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     8821 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     8881 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     8941 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     9001 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     9061 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     9121 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     9181 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     9241 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     9301 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     9361 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     9421 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     9481 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     9541 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     9601 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     9661 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     9721 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     9781 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     9841 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     9901 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     9961 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
    10021 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
    10081 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
    10141 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
    10201 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
    10261 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
    10321 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
    10381 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
    10441 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
    10501 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
    10561 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
    10621 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
    10681 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
    10741 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
    10801 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
    10861 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
    10921 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
    10981 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
    11041 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
    11101 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
    11161 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
    11221 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
    11281 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
    11341 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
    11401 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
    11461 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
    11521 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
    11581 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
    11641 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
    11701 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
    11761 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
    11821 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
    11881 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
    11941 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
    12001 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
    12061 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
    12121 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
    12181 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
    12241 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
    12301 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
    12361 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
    12421 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
    12481 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
    12541 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
    12601 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
    12661 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
    12721 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
    12781 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
    12841 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
    12901 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
    12961 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
    13021 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
    13081 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
    13141 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
    13201 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
    13261 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
    13321 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
    13381 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
    13441 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
    13501 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
    13561 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
    13621 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
    13681 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
    13741 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
    13801 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
    13861 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
    13921 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
    13981 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
    14041 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
    14101 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
    14161 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
    14221 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
    14281 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
    14341 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
    14401 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
    14461 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
    14521 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
    14581 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
    14641 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
    14701 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
    14761 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
    14821 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
    14881 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
    14941 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
    15001 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
    15061 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
    15121 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
    15181 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
    15241 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
    15301 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
    15361 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
    15421 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
    15481 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
    15541 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
    15601 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
    15661 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
    15721 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
    15781 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
    15841 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
    15901 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
    15961 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
    16021 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
    16081 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
    16141 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
    16201 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
    16261 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
    16321 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
    16381 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
    16441 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
    16501 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
    16561 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
    16621 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
    16681 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
    16741 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
    16801 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
    16861 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
    16921 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    16981 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    17041 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    17101 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    17161 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    17221 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    17281 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    17341 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    17401 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    17461 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    17521 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    17581 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    17641 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    17701 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    17761 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    17821 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    17881 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    17941 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    18001 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    18061 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    18121 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    18181 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    18241 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    18301 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    18361 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    18421 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    18481 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    18541 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    18601 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    18661 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    18721 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    18781 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    18841 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    18901 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    18961 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    19021 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    19081 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    19141 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    19201 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    19261 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    19321 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    19381 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    19441 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    19501 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    19561 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    19621 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    19681 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    19741 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    19801 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    19861 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    19921 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    19981 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    20041 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    20101 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    20161 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    20221 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    20281 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    20341 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    20401 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    20461 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    20521 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    20581 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    20641 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    20701 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    20761 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    20821 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    20881 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    20941 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    21001 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    21061 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    21121 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    21181 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    21241 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    21301 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    21361 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    21421 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    21481 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    21541 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    21601 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    21661 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    21721 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    21781 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    21841 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    21901 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    21961 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    22021 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    22081 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    22141 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    22201 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    22261 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    22321 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    22381 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    22441 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    22501 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    22561 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    22621 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    22681 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    22741 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    22801 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    22861 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    22921 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    22981 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    23041 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    23101 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    23161 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    23221 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    23281 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    23341 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    23401 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    23461 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    23521 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    23581 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    23641 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    23701 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    23761 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    23821 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    23881 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    23941 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    24001 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    24061 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    24121 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    24181 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa
    24241 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
    24301 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
    24361 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
    24421 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
    24481 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
    24541 tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
    24601 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
    24661 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
    24721 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
    24781 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
    24841 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
    24901 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
    24961 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
    25021 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
    25081 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
    25141 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
    25201 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
    25261 aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa
    25321 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    25381 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    25441 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    25501 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    25561 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
    25621 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    25681 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    25741 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    25801 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    25861 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    25921 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    25981 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    26041 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    26101 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    26161 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    26221 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    26281 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    26341 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    26401 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    26461 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    26521 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    26581 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    26641 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    26701 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    26761 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    26821 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    26881 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    26941 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    27001 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    27061 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    27121 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    27181 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    27241 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    27301 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    27361 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    27421 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    27481 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    27541 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    27601 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
    27661 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    27721 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    27781 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    27841 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    27901 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    27961 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    28021 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    28081 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    28141 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    28201 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    28261 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    28321 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    28381 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    28441 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    28501 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    28561 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    28621 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    28681 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    28741 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    28801 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    28861 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    28921 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    28981 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    29041 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    29101 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    29161 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    29221 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    29281 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    29341 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    29401 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    29461 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    29521 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    29581 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    29641 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    29701 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    29761 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    29821 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    29881 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    29941 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    30001 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    30061 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    30121 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    30181 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    30241 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    30301 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    30361 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    30421 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    30481 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    30541 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    30601 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    30661 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    30721 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    30781 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    30841 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    30901 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    30961 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    31021 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    31081 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    31141 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    31201 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    31261 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    31321 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    31381 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    31441 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    31501 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    31561 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    31621 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    31681 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    31741 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    31801 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    31861 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    31921 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    31981 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    32041 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    32101 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    32161 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    32221 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    32281 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    32341 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    32401 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    32461 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    32521 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    32581 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    32641 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    32701 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    32761 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    32821 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    32881 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    32941 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    33001 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    33061 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    33121 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    33181 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    33241 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    33301 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    33361 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    33421 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    33481 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    33541 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    33601 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    33661 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    33721 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    33781 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    33841 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    33901 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    33961 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    34021 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    34081 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    34141 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    34201 gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat ttctgataat
    34261 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    34321 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    34381 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    34441 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    34501 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    34561 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    34621 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    34681 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    34741 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    34801 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    34861 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    34921 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    34981 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    35041 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    35101 ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga
    35161 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    35221 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    35281 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    35341 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    35401 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    35461 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    35521 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    35581 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    35641 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    35701 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    35761 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    35821 gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct
    35881 gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat
    35941 ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc
    36001 tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa
    36061 ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca
    36121 ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg
    36181 atgctagcag cagcttttgc ttcattcttt actggtgtga cagaaccact tgaattttca
    36241 tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta
    36301 gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat
    36361 tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga
    36421 ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg
    36481 atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca
    36541 gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat
    36601 gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg
    36661 atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa
    36721 aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta
    36781 catggtggtg ctgcagcacg acctgcacaa acaaacaccg tggttaaaac agaaacattt
    36841 gccggtgaaa cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc
    36901 actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta
    36961 ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg
    37021 aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc
    37081 aacactgttg acttaaaagg cgaaccattc actttatatg tagaagaagg tcaaaaagtc
    37141 gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac
    37201 acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc
    37261 gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc
    37321 gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca
    37381 gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagct
    37441 aaacgaaaca tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca
    37501 gctttttcac aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta
    37561 tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc
    37621 ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt
    37681 ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga
    37741 caaacgctgc atcacttgct tcaataattt ctaactcttt acctgtcgca cgctctaatg
    37801 ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt
    37861 cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg
    37921 tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag
    37981 gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct
    38041 taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat
    38101 ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat
    38161 tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc
    38221 ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc
    38281 cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt
    38341 gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg
    38401 ttactaaata atgcatattt gccactccct attcttttta tttgttaaga tttacagctg
    38461 tacatttata aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa
    38521 atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt
    38581 gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt
    38641 atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg
    38701 atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct
    38761 ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa
    38821 acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct
    38881 cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat
    38941 aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt
    39001 ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt
    39061 tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa
    39121 agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt
    39181 tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca
    39241 aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt
    39301 ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat
    39361 aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc
    39421 ttaattcttc aggcgattct gctaaagcgc ttacaacaag atacgttcct tttgggaatg
    39481 ggatgattcg cgtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca
    39541 ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca
    39601 atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca
    39661 aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat
    39721 ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt atattaagtt
    39781 catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt
    39841 tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa acattactga
    39901 aatcctctcc ccattttgta aaagagatct cgggagcttc gactaaccca taatctgaag
    39961 aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg ggaaatggcc
    40021 ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct aaatcagcgt
    40081 acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc ttttcttcca
    40141 agcggtctcc atcaaatcca taagaaacgt tgattacttg atttggatat aaatctccta
    40201 acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt tgtatttctt
    40261 gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca taaaactgtt
    40321 ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata aaaaatgtca
    40381 agcgtggaaa agatttttgt atgccgtatc tactgatttc tgaaggcaga tatcctgacc
    40441 aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta aacgccgttt
    40501 ctgtcactct tgatccatct agaagatcgc gattagccaa agccaatcta cggtttttga
    40561 tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat tgatattcag
    40621 agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa tgttcttcga
    40681 tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt gattgccttt
    40741 ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt tagtcagatg
    40801 tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa cggataattg
    40861 gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga agacagattc
    40921 taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct tactacctac
    40981 tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat ttttctttgc
    41041 gatacatcat acctctttta tcaattggaa caattattta tttctaacaa aatcacttca
    41101 tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta
    41161 ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa
    41221 agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat
    41281 ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga
    41341 aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta
    41401 tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac
    41461 ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg
    41521 caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta
    41581 ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct
    41641 tttaacagag attcttatag aacatccgca ctttatcgaa cgcttcaaag ttaagatgcc
    41701 atttcatgca ataaattatt cagataaatt aacaactatc atccataaat acgatcatat
    41761 tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat
    41821 ccaagaagca gttgaatcgc ttattcagca aaatgcctat cttcccgcat tcgaattgat
    41881 caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct
    41941 tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc
    42001 agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta
    42061 ttcaaaacgc tactctataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa
    42121 ccaaaagaaa aaaatagata aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa
    42181 aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc agaccttgct
    42241 ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta cccaatatta
    42301 gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt ccttcgttgc
    42361 tggatagatt ttccggcatt gttgatagat tttatgatac ttttctacat tttgtaagag
    42421 aggttccgct gcatctgtat attggataaa attctgacaa caactttcag gtgtgtcgaa
    42481 ccaccctaac cctagtgcag caatcattgt tgcgcctaat gcaggtcctt cctccatact
    42541 taaacaatag accgttgcat cgaaaatatc agcttggatt tgtagccatg caagattttt
    42601 cgcgcctcct cccaccgaaa caatacgtgt aaatctgcga cccgctagtt ctttacgttt
    42661 caatcccacc attgacaaat gctcattaaa aatttagaat attcacaagt ttaacgacaa
    42721 ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa
    42781 aactgcgatc aaagacacgt tacctaccgt tttcggttat atcggtattg gacttgcatt
    42841 tggtatcgtt gggaaagctg ccggatttca tccactagtc gtcacgttga tgtccctgct
    42901 ggtctatgct ggttctgccc aatttatcac agtcagcatg cttgctagtc acagcccatt
    42961 gctttccatc gttttctcga cctttctagt caattcccga atgattctga tgagtatgac
    43021 gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct
    43081 gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa
    43141 ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct
    43201 tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc
    43261 catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc
    43321 taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat
    43381 ttttatccca agcaacttag tgattgtagt tgtaacactg attgggtgcg gattgggggt
    43441 gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt tgtactttag
    43501 caacttggct ttctagggtc ctaccatttg tcttactgaa aaaattcgat ttaccgcaac
    43561 cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca
    43621 gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt
    43681 cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc gtcttggcag
    43741 gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa
    43801 attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct
    43861 tgcttatccg ttgaacggac cactaacaac gacttttcct aacatatgtc ctgtttctac
    43921 gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa
    43981 tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg
    44041 cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat
    44101 agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac
    44161 atgtccaaat ggtcggacaa ggtgcttgat ttgatcaaag tagcgggtaa tatcgaataa
    44221 tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg
    44281 atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact
    44341 tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac
    44401 gcccccggat ccgttgatca ctaagatttt tttcccgcga ttggcatttt cttctggagt
    44461 tagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc
    44521 agcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt caactagttg
    44581 gtattcttga ttgcttcctg ctcttgttgt tgtacctgcg taaaaaacgc gatcgccgac
    44641 agaaaatttt tgcacttcgt cacctacagc aacaactttc ccgactccat caaaacctaa
    44701 gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac
    44761 agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt
    44821 ttcatcaata aagctttttg gatcatcgat cggcaaccct tcataaaaac cgactacctt
    44881 cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat
    44941 acagtgttca tttcaatata tatagtatgc tatttgttcc acctttttta ttcatcataa
    45001 ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga ggttgcgaca
    45061 agacttttgt cacaacctct tataaacggt gttctcctac caactcttcg gtcacaagtc
    45121 aaaatattca tcaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagtta
    45181 aacggcaggc tcacaacctc tgatataaac ggtgttcaat caactgaccc tcggtattag
    45241 ctcaaaaaac gacaaaatat gaagagctat ttttgtttgg ctgttcttat tacttgatac
    45301 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
    45361 aaaagcgttg gatgttcttt ttcaaaggtc tctaagatct ctcgtacttc atcagtagat
    45421 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
    45481 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
    45541 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcgggtct
    45601 ttttcaaatg cataaggatt cttgaaaatc cctcggccaa tcatgatgcc atcaacgcca
    45661 tatttctcgg ccaattcttc tcctgtctgg cgatcgggga tatctccatt gatcgtgatt
    45721 agtgtttgtg gagcgatttg gtcacgtaaa gcaatgattt taggaatgac ttcccaatgt
    45781 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
    45841 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
    45901 gtcttgacgc tgacaggcaa accgccagct ttagctgctt caatcaattg tgccgctact
    45961 tctggacgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
    46021 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
    46081 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaat
    46141 gttaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
    46201 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
    46261 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
    46321 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
    46381 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
    46441 gtttctgatt gagcaaaaaa aagactgtga caaaaatctt atcacagtcc attttccgga
    46501 ataaatggtg ttcaaaagct tgcatttgcg gtaataagtc caaccaaacg aaaaatagga
    46561 ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc
    46621 tccactaaac ggtgttcaat cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat
    46681 gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    46741 tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa
    46801 aacaacgatg aataacacaa gtacgatcat cgatgttgct actggcacat cgatcgttcc
    46861 aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt ttgaaacagc
    46921 ttgccaaaag ggcggtaaca tagagatggc atagaaaacg ccacctagat aagttaacgg
    46981 ctgcaagaca aatgtaggca cgatcgatac atcatcataa gactgagcaa aaatcccgtt
    47041 gagcagtcct gctaatgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa
    47101 ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac
    47161 gagaatactg cgacccacac caccaatcac aaagccccaa ataatcacat gcgtaggaac
    47221 aggtgctacc aagatttctt cgatattttt ctgaaatttt tgagagaaga aggacgaaga
    47281 aacattggca tacgaactag tgatagcaga catcataatc aaaccaggaa cgatgaattc
    47341 catatatgaa aaaccaccca tgtcaccgat ccgcccaccg atcatttttc cgaaaataac
    47401 aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt tggacccata tacgcaaata
    47461 gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt
    47521 tctcctccgt atgattattt tcttctgtga ttttcaagaa cagttcttct aaacgattcg
    47581 acttattcct catagaaaga actttgattc cttgtcttgt aagctgatcg aagattccgt
    47641 tcaccccttg atttcgttca acttcgacag cgattgtctg ctcatcttca aatgtacaag
    47701 catatccttc aatcacaggt ttggtatcat atggcgctaa atcaaagata aatgtttcaa
    47761 attgcagttt tgacagaagc tctttcatac ttgtattctc gatcagttca cccgattgga
    47821 tgattccgat gttgcgacaa agcatttcag cttcttctag ataatgcgtc gtcaaaatga
    47881 tcgttgtacc attttcgttc aattctcgta gaaactccca catctcgcgt ctcaactcga
    47941 tgtcaactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc
    48001 tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct
    48061 tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag
    48121 agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca aaagggttaa
    48181 aattgaactc ttgtggaacc aatccaatct gttgctttgc tcgaactaga tccttgtcca
    48241 gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa gtaataatcc
    48301 cgatcgttgt agacttccca gctccatttg gccctaatag cgcatagaaa tctccttctt
    48361 tcaccgaaag atcgattccc cgcaatgctt cgaccccact tccatagatc tttttcaaat
    48421 tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat gattcttaat
    48481 tgtatcatgg aatacagcag atgaaaaaac aacttggctt ccacacaaaa acacacctgc
    48541 taacaaagag gagatgtgtt ttctagttca tgatcatctt cgatagaata actatctaaa
    48601 caactctgga acttttaaaa tcataaaagt accgacagca actactacga tgacatatag
    48661 aagatcctta taactgaggt tcttgtcttt tttattcatg ttaagctttc ctccgtcctt
    48721 ttatcgaaag cttttttctt ttattttaac cgaaataaat aaaatggaaa tagttatttt
    48781 caatttctgt tttcgctgtt tgttttacgg agaactttgc catggaacgt gcgtctcgtc
    48841 gctgatgcta agttttttcg ataaaagctg ctagaaggaa gacagcgtgg tttattttgt
    48901 atcgtctttt gctaaatctt ccaagatcat ctggacagca gactcgttcc cattgaattc
    48961 tgccactttt ttggcatcaa tgtacttctt ttgataatcg tctttttgga cccacgcatc
    49021 atgcttcgtt tgataaagtt aatcaaacgg ataccactga cgctgttcat cgaacaacaa
    49081 actttctttt tgggcctgtt caactatgga tttaggataa ttcaaaactt tcagcaaggc
    49141 aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt
    49201 tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt
    49261 caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac
    49321 gactgaagcg gaagaagcaa tccgttcgat cgtatttgtt ccttttaaaa tctcatctac
    49381 gaagcagtag cagcgttctt tggaagccac ttggtccagc aaacgtttga tcgatttgat
    49441 ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa
    49501 cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag
    49561 aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt
    49621 gactaatgta ttcttctgcc agttgactgg attaactacc gcttctttta acaatggatg
    49681 atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatcggcat
    49741 ataggtgcga taattcaaaa cggctgctgc aacttccaat tctcccagca attcccacag
    49801 cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa
    49861 tggcagcatg aacatgatgt tgacatattc aaaaatcaat tctgcttcac tcccgctctt
    49921 cattcgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag
    49981 ctctttttga agtggtgtcg tcatttttgc taactgattg gcggctgaaa tcgttgaaac
    50041 caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac
    50101 cgtattgaat actaagccac cgattgctag atagattcct agtacttgcc ctaaaagaag
    50161 caaacctaca cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat
    50221 ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa
    50281 acgagcgaat tgatattgga ttctttctct cagttgagga ttttcctgat aaaaagcaat
    50341 tagtttttct aactgataat cttctccaaa atcaaagcta cgtaaacgct gatacaacgc
    50401 ttctgaacct acactagaat aggtactgtt gatcccttca aatacttcga acatatccaa
    50461 gtcataccaa gtaagatcgt cgatttcact atcccatgaa cggaattttt tctccgtgag
    50521 ccacgcatcc ttcaaacttt cttctttatc aaatctcggt tgataaggaa tttttcccca
    50581 attactacga actgtttcct ttactttcaa acgtgaaaaa atgtctaaaa caactaataa
    50641 aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat ccctctccgt
    50701 tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa
    50761 taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa
    50821 agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta aattagagcc
    50881 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
    50941 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
    51001 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
    51061 gtttgcgctc ctggtttgat aggattggcc actgttaatc gaatgaatga atcctctttt
    51121 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
    51181 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
    51241 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
    51301 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
    51361 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
    51421 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
    51481 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
    51541 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccac ccatgtattc
    51601 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
    51661 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
    51721 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca cttaatcaaa
    51781 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagtttccc
    51841 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
    51901 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
    51961 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
    52021 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
    52081 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aacagttctt cgatatcaaa
    52141 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
    52201 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
    52261 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
    52321 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
    52381 aagaacctcc tccagtaaag aatgaacagc ttcatcgtct tcgataatca aaatagtttc
    52441 cagcaggaac acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
    52501 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
    52561 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
    52621 gcctttgatt tttgcgaaaa caccgggaac gaaggccgtt cccatgatat aaagaaaaga
    52681 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
    52741 tgtcgctaat atcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
    52801 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaatca tggctagccc
    52861 cgtaatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
    52921 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
    52981 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
    53041 ttccaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
    53101 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
    53161 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
    53221 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
    53281 gcccaactag gtggatacca tccacaggaa cttttgcctc taccaatgct tgattgatgg
    53341 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgg acaaaataat
    53401 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
    53461 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
    53521 cttcatgcaa aataccgtag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
    53581 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
    53641 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
    53701 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
    53761 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
    53821 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
    53881 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
    53941 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
    54001 tagacttacc cgctccattc tccccaatca aaccatagat cattccacgt ttgatttcga
    54061 tcgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
    54121 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgataa gtgaagtata
    54181 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
    54241 aatttcaaga aaaatcagac tgtctcgcag acgtggttga gttgtctgcc aatcaaagag
    54301 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
    54361 catcagacgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
    54421 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
    54481 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
    54541 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
    54601 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    54661 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
    54721 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
    54781 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
    54841 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
    54901 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
    54961 tgcattttac aggaatgtat ttcatgcgaa attaataagt ctttgtttat agagttttac
    55021 tagggtataa aaagttgtat gttatcatta cctcaaaaca ttacctcagc ttggcgagtt
    55081 ggttgattga agaataaatc atttatctag atatctcaat aaaaaatgat ttctaagaaa
    55141 cttgtatttt cgtgttatta tttttttgcc ctatcaaaat ttctaattct tctagcgtaa
    55201 tatgattatg acacataatc gattcaatta cttcaatacc tatttttttc gagaaagatc
    55261 ccttttgttc actcacaaaa atactttcag aagacatata taaggaacga agttttggaa
    55321 ttagtatatc gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa
    55381 ttaaatttgc tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa
    55441 caatgtctac ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat
    55501 tacttataaa tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa
    55561 tattttgttc atcagccaca ttccccataa atccgccgat tccttttaag taataattct
    55621 cacctaagag tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg
    55681 gaaaaataaa ttctgatcga actttattta tatttttagc tcctattaat tttataactc
    55741 gattaatgtt cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa
    55801 aaaaacaact ttaacatatg catcattaat tcgttatttt ttaggtagat attgatcatt
    55861 gttatctctc tttttttgct tcccatactc caacttagaa attgccgtta taaaatttat
    55921 tttaaaggtg actttttccc ctgaagtatc ggtagtagga atacttaata cctcagcttc
    55981 tgaggaggta tcccaattat agttatactt tgcatcatca ccataaagaa caattccatt
    56041 ttttttatct tgtgccgtta gctttactgg ttcgcttact tttcttgtta atatcttgga
    56101 ctttacatca tcgatattgc ttattttatc aagcttaaat aatttacaaa ttaatttcgt
    56161 actagttttt atatttgacg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc
    56221 ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc catatgaacg
    56281 att
//
LOCUS       DAPBAB010000014        51992 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPBAB010000014 DAPBAB010000000
VERSION     DAPBAB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 51992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.014"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(20..595)
                     /locus_tag="RD444_001038"
     CDS             complement(20..595)
                     /locus_tag="RD444_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016614053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7548427.1"
                     /translation="MGQKMINVKPIKNKEVLNQFGKELKKGKHGKRDYLIMAIGIKTG
                     LRISDILNLKVSDVKNKTQTEIIEQKTGKRRTLHLNRLTVSIIEYLNEEHDSQSEWLF
                     PSPSDRSKPLASHQYYKIMQKVADSMGLDYIGTHTLRKTFSYFFLKHNRGDIVTLMKI
                     LNHSSQAVTLRYSGIEEDDIKAKLEDFDPFK"
     gene            849..2012
                     /locus_tag="RD444_001039"
     CDS             849..2012
                     /locus_tag="RD444_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7548428.1"
                     /translation="MAKIEKYIKKNGATAYKFRLFLGLDYLGRQKYIKKSGFPTKKQA
                     LEKLAEIEGKIESEQKYIQLSFQDMYKEWLITYKDSVRLVTYNRTIDLFRLKILPIFG
                     KKYVDEITTEFCQQILNKWAKEYHKYKALKGYTQQILDLAVKKDLIQKNPMRLCTMPK
                     INKSRMNIANTVELSSDNTENYYDKNELNNFLDVLEQNYDYMWFTAFRLLAFTGMRKG
                     ELMALRWSDIRADSIIVNKSMTMIKRIAYVSDTKNEQSNRVISIDPTTSNILKTWKEK
                     QQARYINVKDNHLVFTRDIPLKNEKDQPFDPDYLNRCMKTVTKENKEIREITIHGFRH
                     THCSLLFEAGATLKEVQDRLGHANSDITLKIYTHVTTETKRETANKLALYLDS"
     gene            complement(2374..2766)
                     /gene="rpsI"
                     /locus_tag="RD444_001040"
     CDS             complement(2374..2766)
                     /gene="rpsI"
                     /locus_tag="RD444_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDT7548429.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2782..3225)
                     /gene="rplM"
                     /locus_tag="RD444_001041"
     CDS             complement(2782..3225)
                     /gene="rplM"
                     /locus_tag="RD444_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDT7548430.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3744..4211)
                     /locus_tag="RD444_001042"
     CDS             complement(3744..4211)
                     /locus_tag="RD444_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDT7548431.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4331..5869)
                     /locus_tag="RD444_001043"
     CDS             complement(4331..5869)
                     /locus_tag="RD444_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDT7548432.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5949..6635
                     /locus_tag="RD444_001044"
     CDS             5949..6635
                     /locus_tag="RD444_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDT7548433.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6836..7363)
                     /gene="msrA"
                     /locus_tag="RD444_001045"
     CDS             complement(6836..7363)
                     /gene="msrA"
                     /locus_tag="RD444_001045"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7548434.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7447..8124)
                     /gene="pgmB"
                     /locus_tag="RD444_001046"
     CDS             complement(7447..8124)
                     /gene="pgmB"
                     /locus_tag="RD444_001046"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDT7548435.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8121..9251)
                     /locus_tag="RD444_001047"
     CDS             complement(8121..9251)
                     /locus_tag="RD444_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7548436.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9251..11434)
                     /locus_tag="RD444_001048"
     CDS             complement(9251..11434)
                     /locus_tag="RD444_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDT7548437.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11431..12456)
                     /locus_tag="RD444_001049"
     CDS             complement(11431..12456)
                     /locus_tag="RD444_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7548438.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12501..13328)
                     /locus_tag="RD444_001050"
     CDS             complement(12501..13328)
                     /locus_tag="RD444_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDT7548439.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13325..14392)
                     /locus_tag="RD444_001051"
     CDS             complement(13325..14392)
                     /locus_tag="RD444_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDT7548440.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14418..15254)
                     /locus_tag="RD444_001052"
     CDS             complement(14418..15254)
                     /locus_tag="RD444_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7548441.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15251..16120)
                     /locus_tag="RD444_001053"
     CDS             complement(15251..16120)
                     /locus_tag="RD444_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7548442.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16190..17473)
                     /locus_tag="RD444_001054"
     CDS             complement(16190..17473)
                     /locus_tag="RD444_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDT7548443.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17487..19172)
                     /locus_tag="RD444_001055"
     CDS             complement(17487..19172)
                     /locus_tag="RD444_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDT7548444.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19447..20472
                     /locus_tag="RD444_001056"
     CDS             19447..20472
                     /locus_tag="RD444_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7548445.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21067..24741)
                     /gene="rpoC"
                     /locus_tag="RD444_001057"
     CDS             complement(21067..24741)
                     /gene="rpoC"
                     /locus_tag="RD444_001057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDT7548446.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(24799..28416)
                     /gene="rpoB"
                     /locus_tag="RD444_001058"
     CDS             complement(24799..28416)
                     /gene="rpoB"
                     /locus_tag="RD444_001058"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDT7548447.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29144..30112
                     /locus_tag="RD444_001059"
     CDS             29144..30112
                     /locus_tag="RD444_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7548448.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30130..31035
                     /locus_tag="RD444_001060"
     CDS             30130..31035
                     /locus_tag="RD444_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548449.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            31032..31838
                     /locus_tag="RD444_001061"
     CDS             31032..31838
                     /locus_tag="RD444_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7548450.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31974..32963
                     /locus_tag="RD444_001062"
     CDS             31974..32963
                     /locus_tag="RD444_001062"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDT7548451.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33280..33804)
                     /locus_tag="RD444_001063"
     CDS             complement(33280..33804)
                     /locus_tag="RD444_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDT7548452.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34091..35902
                     /gene="scm"
                     /locus_tag="RD444_001064"
     CDS             34091..35902
                     /gene="scm"
                     /locus_tag="RD444_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDT7548453.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(36057..37043)
                     /locus_tag="RD444_001065"
     CDS             complement(36057..37043)
                     /locus_tag="RD444_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDT7548454.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(37066..37632)
                     /locus_tag="RD444_001066"
     CDS             complement(37066..37632)
                     /locus_tag="RD444_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDT7548455.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(37646..38062)
                     /locus_tag="RD444_001067"
     CDS             complement(37646..38062)
                     /locus_tag="RD444_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDT7548456.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(38519..40453)
                     /locus_tag="RD444_001068"
     CDS             complement(38519..40453)
                     /locus_tag="RD444_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7548457.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            40734..41690
                     /locus_tag="RD444_001069"
     CDS             40734..41690
                     /locus_tag="RD444_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDT7548458.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            41780..42883
                     /locus_tag="RD444_001070"
     CDS             41780..42883
                     /locus_tag="RD444_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDT7548459.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            42896..43849
                     /gene="menA"
                     /locus_tag="RD444_001071"
     CDS             42896..43849
                     /gene="menA"
                     /locus_tag="RD444_001071"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDT7548460.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(44784..46130)
                     /gene="gor"
                     /locus_tag="RD444_001072"
     CDS             complement(44784..46130)
                     /gene="gor"
                     /locus_tag="RD444_001072"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDT7548461.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(46264..47517)
                     /locus_tag="RD444_001073"
     CDS             complement(46264..47517)
                     /locus_tag="RD444_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDT7548462.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(47523..48446)
                     /locus_tag="RD444_001074"
     CDS             complement(47523..48446)
                     /locus_tag="RD444_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDT7548463.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(48667..51159)
                     /locus_tag="RD444_001075"
     CDS             complement(48667..51159)
                     /locus_tag="RD444_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDT7548464.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(51166..51633)
                     /locus_tag="RD444_001076"
     CDS             complement(51166..51633)
                     /locus_tag="RD444_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDT7548465.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(51769..51842)
                     /locus_tag="RD444_001077"
     tRNA            complement(51769..51842)
                     /locus_tag="RD444_001077"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51806..51808),aa:Arg,seq:acg)
     gene            complement(51900..51973)
                     /locus_tag="RD444_001078"
     tRNA            complement(51900..51973)
                     /locus_tag="RD444_001078"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51937..51939),aa:Pro,seq:tgg)
BASE COUNT        15038 a        10968 c         9524 g        16462 t
ORIGIN      
        1 cttaatcgac tgtgcctttt tatttaaacg ggtcaaaatc ttctaatttc gctttgatat
       61 cgtcttcttc aataccagaa tatcttaaag ttactgcttg gctactgtgg tttaaaattt
      121 tcattaatgt tacaatatct cctcgattat gctttagaaa gaaataagaa aaagtctttc
      181 ttagtgtatg tgtgccaata taatctaacc ccatactatc agctactttt tgcatgattt
      241 tgtaatattg atgacttgcc agtggtttac ttctatctga aggacttgga aataaccatt
      301 ctgattgaga atcatgttct tcatttaaat attcaataat agatacggtc aatctgttta
      361 aatgcaacgt tcttcttttt cctgtttttt gttcaataat ttctgtttgt gttttatttt
      421 tcacgtcaga aacctttaaa ttaagtatat ctgaaatccg taaccctgtt ttaattccga
      481 ttgccatgat tagatagtct cgttttccat gcttcccttt ttttaactct tttccaaatt
      541 ggttcaagac ctctttattt tttattggtt ttacatttat cattttttgt cccataattt
      601 gcctccctta tttctatttt ggtggatact tttattaatt tctgatacaa aagtatccat
      661 caccctataa atcaaaaatg aaacattaat agaataggat tcgtgatgga tactaattga
      721 agaagtatcc ctttcatggt ccgttatagc atcacaattt gatggttgca aatttgtaga
      781 acatcctcga tatccactct gaagtgaggt ataatcgctg cacacttctt cagaatggag
      841 cgattggttt ggctaaaata gaaaaataca taaagaaaaa tggagcaact gcctataaat
      901 ttaggctttt ccttgggtta gactaccttg gtagacaaaa atatattaaa aaatctggat
      961 tcccgacaaa aaaacaagca cttgaaaaac tagctgaaat agaagggaaa attgaatctg
     1021 agcaaaaata tattcaatta tcatttcagg atatgtataa agaatggttg attacctata
     1081 aagattctgt cagattggtt acatacaata gaacaattga tttgtttaga ttaaaaatat
     1141 tacccatttt tggtaaaaaa tatgttgatg aaataactac agaattttgt cagcaaatac
     1201 taaataagtg ggcaaaagaa tatcataaat acaaagcttt aaaaggttac acacaacaaa
     1261 ttcttgattt agcagtaaaa aaagatttaa ttcaaaaaaa tcctatgcga ctatgcacaa
     1321 tgccaaagat aaataaatct cgaatgaata ttgctaatac tgtcgaatta tcaagtgata
     1381 ataccgaaaa ttactatgat aagaatgaat tgaataattt tttggatgtg ttggagcaga
     1441 actatgatta tatgtggttt acagcttttc gattattggc ttttacgggg atgaggaaag
     1501 gcgaactgat ggctttaaga tggtctgata tacgtgctga cagtattatc gtaaataaat
     1561 caatgacgat gattaaacgc attgcttacg tatcagacac taaaaatgaa caaagcaata
     1621 gagtaattag catcgaccct acgacttcta atattttaaa aacttggaaa gagaaacaac
     1681 aggcacgcta tattaatgtt aaggataatc atttagtatt tacaagagat attccattaa
     1741 agaatgaaaa agaccaacca ttcgacccag attatcttaa tagatgtatg aaaacagtaa
     1801 ccaaagaaaa taaagaaata agagaaatta caattcacgg atttagacat actcattgtt
     1861 ctctattatt tgaagcagga gcaacactca aagaagttca agaccgatta ggacacgcta
     1921 atagtgatat aactttaaag atttatactc atgtcacaac tgaaactaaa agagaaacag
     1981 caaataaact tgctttatac ttagatagct aagaagctag ttcatacgaa ttagcttctt
     2041 ttttagttca acaaaaataa attttttatt cacctattaa agactttcta tacattctaa
     2101 accaacaaaa gatacataaa acacaatgtt ttacgtccca aaggtaaaaa tcataattcg
     2161 ataacaattt tatactttcg cattacctaa cactgctaaa aacatagtaa acattagaat
     2221 taaataaaat ttaaaaataa cagatttttc caagtctcga aaacagttta tatagaaaag
     2281 ggtctgtaaa atagacccat tttttaagaa atttagctaa aaaaagacac catccaaata
     2341 gatgatgtct tgaaatcctt gatgtaataa ggattaacgt tttgaaaatt gacttgcttt
     2401 tcttgccttt tttaaccctg gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa
     2461 gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg cacgagcgat
     2521 accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt taacaaatac
     2581 gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt cacgcaagtc
     2641 agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc cagttcctgg
     2701 tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat attgaacttg
     2761 tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt ctggttgttg
     2821 tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc
     2881 taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac gagaattttt
     2941 agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc tgtgacggta
     3001 ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat tgatgacgat
     3061 gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac cacgtagtac
     3121 agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta ctacatacca
     3181 tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat tttcctccaa
     3241 tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg gggcaaacaa
     3301 taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg cttgattgaa
     3361 aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg tttcctgtca
     3421 aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta ttttgaataa
     3481 acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa cataagaagc
     3541 tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca acctcttggt
     3601 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     3661 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt
     3721 tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc caaatgagct
     3781 tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat atcttgtgtt
     3841 ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc tgctagataa
     3901 cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca gcggccaatt
     3961 tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg tgaaccgtct
     4021 aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc catgaactca
     4081 tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg tgtttggtga
     4141 acaacaacag acatttggct tagatctgca actaattgat ttaatacttc tttggtttgt
     4201 tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat agtagacttt
     4261 caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc ttttttatcc
     4321 gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca aattgctgat
     4381 aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc tgatattttt
     4441 tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc tgtgcttcac
     4501 tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct aatgcggatg
     4561 cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc atctgacgaa
     4621 aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga ttttcctggc
     4681 aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc gccttcatca
     4741 tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca tctgcgtccc
     4801 aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg gcacccgctg
     4861 gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt tctttagcag
     4921 attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc
     4981 tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac gtctccattt
     5041 ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt cctacggtca
     5101 aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc ccgtctgccc
     5161 ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt tttgatggta
     5221 gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca
     5281 agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat ccagttcctg
     5341 aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata tagtctttga
     5401 taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc agccacttga
     5461 ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa aattctttgc
     5521 caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct gcccaagtga
     5581 tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc atcgcagagg
     5641 agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact tcttttataa
     5701 cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct tgtgtttctt
     5761 ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa
     5821 ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt ttttcacctc
     5881 aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt tgaaaagggg
     5941 gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt ttatgtttaa
     6001 ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat tgtgtcaaat
     6061 gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc gtccaacgat
     6121 ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca gaagccttat
     6181 gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt tcgacgttca
     6241 tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc atggtccatt
     6301 ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag gccggcgtct
     6361 tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc
     6421 aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt ggcttgtctc
     6481 tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc gttttttctg
     6541 tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct tttttgagca
     6601 gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca aacgacttgt
     6661 cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag ctaggcgaca
     6721 ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat tagctcaaaa
     6781 tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg gcttcttatt
     6841 tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct cgataaagat
     6901 tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca ggataaaatt
     6961 cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg
     7021 cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag atcactggac
     7081 gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc
     7141 aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg atctctactg
     7201 cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt ggtacatgcc
     7261 cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat ggtttgacca
     7321 tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat cccccttttt
     7381 tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg
     7441 aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta cttgatcaaa
     7501 aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt cacctattcc
     7561 caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat cttcaatgac
     7621 caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa cttctggatc
     7681 aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt
     7741 cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga tcttgatgtt
     7801 tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg tttcatccat
     7861 tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg atttctcttt
     7921 ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg actcggttct
     7981 ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca gctggttagc
     8041 taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc catctaaatc
     8101 gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa
     8161 gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt
     8221 aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc tgctgctttt
     8281 tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc tggtactctt
     8341 ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg tccttcgcct
     8401 acgattacga ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc caatacgtag
     8461 tcgatgccag agactatttt ccctgaagca aatgctaaca gtcctgcacc cattccacca
     8521 ccagcactac tgcccgatgc ctcgattact tgaggggcaa atctcatata aaaatcatgc
     8581 atggcttggt cagccactgc taattcattt tctttcaagc ctttttgtgg tccgaaaaca
     8641 aaagaagcgc cttcttttcc gcataaaaga ttagtaacat cagtaatgac agtgattttg
     8701 gctttaatca gctcaatagg cacgttcgtt ttatcaactg tagcaatttt acctagattt
     8761 tctcccagtg cttctacttt ttgattatct tgatcgtaaa aagcataacc tagaccttct
     8821 gccatgccga tgccgccatc gttggtacta cttccgccaa cgccgatgat gatgtgtcga
     8881 acaccttgtt gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga taacctaagt
     8941 ggctgttgtt ggtttagtgg gacatctttc aatccgacga tttctgccat ttcaaaaatc
     9001 gcagtttgac cgtctgaagc gtactggatt tctttgtttt tgccaaatgc attagtaacg
     9061 actgctgttt gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt tccttctcct
     9121 ccatcgccta taggtagcag atcatattca gcctgaacaa aaacttcttg aaaacctgat
     9181 tgaatggctt gtgctgcctc taaggctgtc agactttctt tgaaagaatc tggcgcaatc
     9241 agaatacgca ttattttccc ctccgttcca agatcagttc tttttccagc tggtattggt
     9301 ctccattgac aaaaataggt aatgtttctt tcgtctcttt ttttattact atttgttttt
     9361 ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa attttttccc
     9421 atgctgaagg aagtcttgga gaaatagaaa gagttccatt cgttactgta atacctgaaa
     9481 aaccaaatat aactgctagc catagtgctc ctaatgaagc agcatgaatt ccgtcatctg
     9541 atgaatgcgg atctggtcct aaatcaatca ggcacgcttc ttggaataaa tgatacgccc
     9601 attccatttc ttggatacgg caagcctcta tggcgtgaat cgctttactc aacgatgagt
     9661 cgtggattgt tttgttttca tagtaatgca aattcttaga aacgattggt tgagaaaaat
     9721 actcaggtaa taagtataac agcatgacaa gatctgcttg cttgctgact tggagttcat
     9781 ttacttcgct acgggcataa tcaagtaaaa tagactggct gccaggacgt tctttgtaag
     9841 tggtcaaatc aataactggc ttactgagat acgtgtcatc ttgcggaatc agctgttctt
     9901 tgttaggcac tggcaaatat aacttatcta aaaaattttg acattctttc aaaaaaagcg
     9961 gatcgccagc ttgatattta acagctaagg ccacattata atgcgccaga tagttcgtat
    10021 atgcattatt gtcgatatat tctgtgtatt catctggacc aatgactctg tgtatctcta
    10081 aatgatcatc gacttttaca gctctgctca gccaaaaagc tgctgtttca tgaatgagcg
    10141 tttttccctt tgtttctaaa aacgattgat cctctgttgc ttggacatac tcttgaacgg
    10201 cataagcaag gtctgcactg atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg
    10261 ttccggtttt gatattcaat gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc
    10321 tttcccatgg gaaaagtgca ccttgatagt gataatctct tgctttttgc cgtgcgcctt
    10381 ctaatcgatc ataacggtac agcagcaaat tctttgctac ttcaggaaac tgatataaaa
    10441 agaaaggcag gataaaaatt tccgtatccc agaaaacatg ccctttataa ccttcgccag
    10501 ttagtccctt cgcccctaca ctaacacttg gatcattgcg gggagtcata atctgtaaat
    10561 ggtaagcagc gaagtccatc gctaactgat cgaaagaaga atcgccttgg atcgttatcc
    10621 gatgctgatg ccaaaaatct gtccaataag acatcgactg ctctaaaatt tctgtataac
    10681 taacgctttt ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg
    10741 tagagagcac gatgatcttc tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa
    10801 aggaagcggt gatccggcga ttttttgctt ggaatacacc tgctttattg aatttagctg
    10861 caatcccaat atcaatttta ctttctgttg tctggtagga tgcactgatc aaatcgtcac
    10921 caaatactcg cagttctttt tcaaccaact gctgtgtacc aaaattggtt tgctgtccat
    10981 cgatacccgt tttcacatct acttggcagt cttcggtcaa cggcttgatg accactttca
    11041 gggctgcctg atggagatta ttttgatcag caacacgaca agtaatgact tccacttttt
    11101 ttccttgagc agtttcaatg agcgcatagc gtttaagctc tcccatttct aaatctagtg
    11161 cccgctcgta tgcaagaact ttcatcgtct caagagaaaa atgttcaccg tcgattttta
    11221 tttcccatct gatgacatct gggagattca ctaactcggc tgattctgct cctaaaggtc
    11281 ggttatatac accagctgca aacattcccc ttacttgatt ccgatagtct tcctcctggc
    11341 ttcctctgat accaaaaaat ccatttccaa tggtcatctg actcgcatac ttattgatat
    11401 actctaaatc aaactgtttt tcttctactt tcataaggta ataacttctc ctgtttctgc
    11461 cgaacgatat aaattttcca ccagattctg gatgacatat ccttgccagc catctgcaat
    11521 catctgagtc tggtcaccga gcgcccgatt cacaaaatga gccatactta gttcatgtgg
    11581 gtcattaact ggcgtggtct tttgtaagga aaccagcttt ccatctacat cttcgaatag
    11641 ttcgcctgga tacagattag cacctgcctg atcgccacaa aaagccacat tcagtttgtc
    11701 ttctctgata ttcaaagcaa acgaagtatt cagttggatg atcttgccgt tttctaattc
    11761 cagtgtgcca aaaattgcat cttctactgt gaatttgctt ggatcccatt tcccgaattt
    11821 ccctgaattt tttttcgtac caattttttg gaagctgtac gcccgaacgc tttttacttt
    11881 aggaaatcca gtaatataca tcgctgtatc aagcatgtga atgccataat cgatcaaagg
    11941 cccgcctcct tgaagttctt tgtcgataaa cgttccccag cctggaacac cagaccgacg
    12001 aagcgcattc gcttctacgt aataaatatc cccgagaaca tcaagatgat tcttgatcat
    12061 tgttgcttcg tcagaaaaac gatgctggaa tccataagcc aaaatctgcc cgtttttatc
    12121 ggctgtctct gccatctttc ttgcttgatc agctgtcata gctggtggtt tctcgcacat
    12181 cactgaagca ttagcttcga gggcagccaa tacatgttcg aagtgaaagc gattgggtgt
    12241 acagacactc acgatatcgg gagaaatctc attcagcatt tgtgtaactg atgtatacgc
    12301 atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac
    12361 agcggccaat tctaagacat ctgatctttt ttgataataa gcagcatgta ctttttctgc
    12421 tacttgtcca gcgcctatga ttgctacttt cttcatttat ttctctctcc tttgcttatt
    12481 tacttattgt tattctttct ctattctttc tctaaatcat ttaaatgacg cgctgtttcc
    12541 agacagtttt tcaagtattg caatgattcc tgataaattt tcacttcatt ttctcctcgc
    12601 aaacgacatt catataccat ataaccttca tactcatttt gaagtagcgt ttgaaaatgg
    12661 cgagtaaaat caattgcgcc agatcctggt tggtagcgat gattatctgc taaatggata
    12721 tgacccacat actctttttg cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc
    12781 atatgataaa aatcagcgat gatctgcaca tgtttcaatt gattagagtc aatgtaatga
    12841 cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg ctccaaataa
    12901 ataaccgtac ctgtttcttc ggctacttta tctaaataac gaagtgattc gctgactgct
    12961 ttatagtctc cttctttgct tcggggtgaa accatcggcg gtaatcgata agtgaacatt
    13021 ccccaagctg ctgggataac gattcctttg cctccaacct cgcttagtgc acgcaaaatc
    13081 tcagcaattt gacgtacacc attaagacgc cgttcttcga taaagtcacc gatccaccca
    13141 tcgtagccgt tacaagctgt cactactggg atccctgttt ctttgattgc ctgtttgact
    13201 gtgtcgatat tttctattaa caacttaccg tcgatttcgt attcttcaaa tccacagtct
    13261 ttgataaatc taaatttttc taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg
    13321 aatttcattg ttctcctcct cggacttgaa cgcccatttt gatactttct tctgggtgct
    13381 ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact ggatcaatga
    13441 tttcttcaca atccaagtaa ccgttcatca ataattccca acatgtgtcc tcgattcgtt
    13501 tacggttcca tcttgggtaa tctgggtttg gttcactaga tgctcgagca aagacgattt
    13561 ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa
    13621 aggcaacata agaaatgatc ccgccatagc cgatccctat taaagaagct tgtaatgcgg
    13681 cagcactgcc actcgtttca ataactgaat ctactccacg tttatctgtc aattccttta
    13741 gacgaaaacc gacatctgct ttcataggat ctagcgtgta atcggcaccg tgtttttctg
    13801 ctaattctct acgctgcgct atgggatcga tcccaacgat ggtagtagca cctgcttttt
    13861 tagctaattg aatcgcaatt tgcccgatag cgcctaaacc gaagattgct acagcatcac
    13921 ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa ttgagcagga tcgtaacata
    13981 ctgcattttt ccattgatcc aaccgatcaa gtttacgtag tttataatta tcaacaccgt
    14041 ttgtaatcag cgtttcacga atgggtccat aagtgcagac taactctcct ttttgatact
    14101 ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat catatttcct aattgaaatt
    14161 cgccaaatac cacaccgcgt tggcttcctt cttctcttgg caagaatagg cgccattctt
    14221 ctgaaaattc ttcatcgata aatggactta atcctctaaa gtcgacaatt tctgttcctg
    14281 gttttggagt agcgaaaatt actcggatac gtacttcgtt cttttcgatc tcacggtctt
    14341 cgtaatcttt gaatccggct attcttggtt ctaatgcaac taattttttc atcttttctt
    14401 tcctctttct tttcttttta tccttttacg ccgccttcga ctaaatcacc tttaataaat
    14461 cgttcagata acgcatacat aatgatgact ggtaaagcgg tcaccaatga agcggccatc
    14521 attcgtcccc aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat tggtaaagta
    14581 aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat aatcattcca cgaaaccata
    14641 aagcaataaa cgaagacaga aatgattcca gaaatagcca atggtataat gatccgaata
    14701 atgatctgga tccggctaag accatcgatc attgctgctt cttcaatatc agtcggaatc
    14761 gtgtcaaaat aactttttag catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg
    14821 ataatcagtg ctgtattggt atcatacaaa cctagaccgg aaatgatttt aaataatgga
    14881 acaacgatca aaataccgga aaacatataa acggtgtaaa agctagcatt gattttcgag
    14941 cgagctttga agcgtagttt tgacaaagcg tatgccccgc tgatcccaat cactactgca
    15001 gttacggagg ctatcaagga gatcactaaa ctatttttga aatacatgat gaatggaaaa
    15061 atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt
    15121 tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat
    15181 ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc
    15241 tttttatttt tcatttcaag tccaccttct ttcgtgagag caagacgacc gctgcaataa
    15301 tgatgaacaa tagaatcgat atagctgctg cttttccaag atcattgaac gcaaaggctg
    15361 tctgatacaa ataaacgcct aagatattga ctttattcgt cagtagatag acatctgtaa
    15421 acatatagaa catccagata gatcgtaaaa caatcacagt tgctagtact ggtttgattg
    15481 ccggcagtgt cacgatcttg aacttctgcc aagcatttgc accgtccatt tcagctgctt
    15541 cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat
    15601 agtatctcca gatagcaaac aaaacgacca taaagaagct agcaactggc tggtcgaacc
    15661 acagcggtgc ttcgttgaaa agctgcaacc tatctactaa cagatcattg acgattccat
    15721 aactattatt gaacatatat ttccaagcaa aaatcaaaga aatagatggc gtaacataac
    15781 ttaataaaat cagtgaacgc accgttttcc taaatctgaa cttgcggtta agcaaaatag
    15841 ctacggctaa tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta
    15901 aacctaaaga acgataaaat tcactatcac ttaaaatttc aagataattg cttaatccga
    15961 taaaaacggc gtctgagtta ggattcagcg gcatatcgaa aaaactgatc tgaatattgg
    16021 aaagcattgg aatcagcact aaaacagcta ataaaagcat gcttggtgct aaaagtccca
    16081 tggctaattt taagtcagaa tgtttccttg gacgattcat tgaaacctcc ccctgataat
    16141 ctgccaatag ctatctactt ttttcgaaca ccattggcag aacttcttgt tatttcgtat
    16201 ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt tccgacagtt acttggttca
    16261 ctgcttttcc gatcacacct gagcttgtga tatctcccat tttcgtgaaa ttcttttccc
    16321 cgactagtcc aaagacttgt acttcatcaa aagcactagc aatctcagat ggcagctcgc
    16381 caaaagactt gatgacttca ttttctttgt aaccatccag ttcgactact tgtttattca
    16441 ctggttgagc accaccagga gacatcaaga cccacttttc catgtttttc ggttgagata
    16501 agaaagctgt gaatgcttca gcagcctctt tttgtttctc gtctagacca gaagaaatcg
    16561 tcaatccaga tactgtgcca tagacagctt gatctttttg tgttgggata gcaaaaccga
    16621 tattttctga gttccccgct tcataaacag aaggcaaaat gtaagttgaa tagattgcca
    16681 ttggtgctgt tccattcatg aaagcatctt tgatttctgt cacatcatta gagcctggca
    16741 tcgtgtattg ggataagttt tgataaaacg acaatgcttc tctcatcttt tccgtatcga
    16801 tcgttacctc gccttcatca tccaaaacat ttgctccatt tgacaaagca aattgagaga
    16861 acgcttgctc actcattgtt ccttcagcag taggcatagc aattccatat tgtttattct
    16921 ctttatctgt gaaatgttga gcaattttta aaatatcatc ccaattttct ggttctgaaa
    16981 aaccagcttc tgataatttt tctttgtcat accaaatacc ttgaacccag ccgctaattg
    17041 gtgcagcaat atagcttttt ccatcttctg aacgaacaag gtttttggct ccgtcataat
    17101 aattatcttc tccagcttcc tcgataacgt tttggactgc cgtctgatca atcagttcat
    17161 ctttatccat cactttagca aaatcctgac tgacttccat tacttctggt aatttaccgg
    17221 aacgagctaa cgtaacgatt tttgtattga acgctgattc ttctaccggc acttgcttga
    17281 ttttgatgtc tggattttct ttttcaaaat cagcaaccag ttcattgatc acacttaatc
    17341 gttcctgttc gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc
    17401 tttgactttc accacaagca gtaagaagaa atccagtagc tagcagacag gtagtaacaa
    17461 taattttttt catttttttc cacctcttat tttattattt tcagccacat atattcatac
    17521 ggctggaata tcaactttcg ttcttgactg caatggataa tttgtccaga aaacaggtct
    17581 aggtaatctc cttttggtaa aagataacct gctgattgat tggaaaaatt atggataact
    17641 aagactttct cttccttcgc ttcgcgaata aaactgataa aagaatctgc tgagtccagt
    17701 aagtaaatca aactattcgg atggaaggct ttttcttttt ttcgaataga tagcatctgg
    17761 gtcaattcac catatactgc tgttcttaat ggatctgtct gcaactcttt ctcaatatct
    17821 gctaacaaaa atttttgccg attgattttt cgattctctc ctgttttttc tacgcctttg
    17881 taatcatttc gacttcccag cacactttga atataaattg ccggcacgcc tggtaacgtt
    17941 accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt atctgttgga
    18001 gaacttaacg catctagata cgtagcgttt atctcatatg gactggtcgt tccatctggg
    18061 tttttcttgt agtttactcg ggcaccctct ttcttcagct gttccactaa ttcaaggatc
    18121 gttttttccg gcagaatacc tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa
    18181 aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg tttttaacca ttctttcaac
    18241 ttacttgtat tacccgaacg aatcgcttga agggtcaaag gtggtagcga aaactgatag
    18301 accatctgtg cttcattctc gccattccca aaataactga tattatcttt gtgtggcaca
    18361 tttgtctcag tgatcaatac cgttcctgga gctgcttcat cagcgattgc acggaataat
    18421 tggatgattt tatgcgtttc tggcaggtgt atacaagaag ttcctagttc tttccataaa
    18481 aatcccacag cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa caaaagaata
    18541 tcgatcattt tcaataaaac ttctggattt ttaaaattca agtctacttg atcttcgcta
    18601 aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg
    18661 ctggtccttg gtctagtcac gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa
    18721 tctttgtacg tatcattcaa gtttaaatat tcctgaaacc aagtactttc ggcagacata
    18781 tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc tatatcttcc
    18841 catgtgccca aacggtcgtt gattgcctga taatttacaa cggagaaacc atcatcagat
    18901 gtatatggga aaaatggcaa gaaatgtaca actctaaatg tatctgccaa atacttttga
    18961 taaaatttct taaatgtacg caaagttggt tgctgctctt caaaaaattg atccccataa
    19021 gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc
    19081 tgataaacac tgactttttg ttcaatcata gaggcggttt tctcagacaa atctgagtcg
    19141 cgataaatcg cttgtaaaag ttttgtatac atcggattcc cctttctttt ttgggagcgt
    19201 tcccaacgat ttgatcatat tatggaaaac gcttactgtc aatagcattt ttttaaataa
    19261 ttttgatata cacaaaaata caaataagaa agcttatgta tcaataatta atagcttctt
    19321 aaaaaaatat ggtctctcat gatcatgcaa gctattccta aattttcaaa atcatagaca
    19381 aaaaagaatg tctattgtac tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat
    19441 ataaaattga aacctactat ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact
    19501 gtctccagag tattaaataa ccagccaaat atctctgata aagcaaaagc aagtgtactt
    19561 caagcaatca aagaattgaa ttatgaaccg agcaaaattg caagaggact gtcaggaaat
    19621 ggttttgacg cgattctagc attatctcat cgtacttctc atacgaccta tggcaatcct
    19681 tttttttcag atgttattca atccatttct gccgtttctg aacaaaataa cttcgacttg
    19741 attctacaaa cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa aaagatccaa
    19801 gaaaaaatga tccgaggaat tattatttta agctctacga tggatgaaac acttcttttt
    19861 gaattggatc aatacaatat tcctattgtg gtaatcggaa aagtcggaaa cgactacacg
    19921 aacatctact cagtggacac ggataatttc caggatagtt acgatttggt ggaaacactg
    19981 atcaagaatg gacaccgtca tatcggctgt ctccatgctc cgacagaagt ttatgtatcc
    20041 aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc accacctaga tatccgtaat
    20101 gaatggatta tagacggcgg ctttcgctta gaagatagca tggcagcaat gaatgattta
    20161 atgaagctac gagaaagacc aactgcgatt ttttctaccg atgcattaaa atcattgagt
    20221 atttacaaag tggcagaaca atctggaaag cagatacctg atgatctttc gatcgcagga
    20281 ttcagtgatc ccttattgtc ctccctattt tcgccacagt tgactcggat agacattccg
    20341 acaaatcagt taggaatcaa agccacagaa cttttattcc aactaatcca ccaccaatac
    20401 cctgaagaaa aaagaatcat tctttcaacc gagattactc tcacagattc gatccaacaa
    20461 caagaaatgt agaacagaat tataaaagaa cagaaacact gaggataagt cagttactct
    20521 gttctttttt caatcactca tccctcgtta ccccctcccg gtattttagc gaatttttct
    20581 tattttccat ctgttttaga tagctgtgga taactataat gtttgtgtta aactattttt
    20641 tccacaggga agaaaaaact atttttaacc acgttgtgta taagttttta tagttcgttt
    20701 ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac
    20761 ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat
    20821 agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa
    20881 aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc
    20941 ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa
    21001 attattttag acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta
    21061 aaatatctat tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc
    21121 tgaaatactg tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata
    21181 acgttgcata ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc
    21241 aagaagagga tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg
    21301 gaatgatgca gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa
    21361 gacaggtcga gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt
    21421 aaagtctgcg atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac
    21481 acgaacttta cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac
    21541 cccttgcata cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac
    21601 atcgcgaact tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg
    21661 gtggatcata tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg
    21721 tgtatctgtt tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat
    21781 atcgatgaca tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc
    21841 aaagatttct tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt
    21901 atggaatgta cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt
    21961 accaactgct tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg
    22021 tttgcagaca ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac
    22081 acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat
    22141 caattcattt gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc
    22201 ttctaatgat tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc
    22261 agtaccgcaa tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt
    22321 caagtaacct gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg
    22381 agttgagata aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa
    22441 ttccatgatt cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa
    22501 gttggaaatg ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc
    22561 caaaccttcc atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac
    22621 acgttcataa cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc
    22681 aacttgttta tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc
    22741 gatccctact gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc
    22801 aagcatctta gaagtttctg ttactttgaa tcgtttgaag acttctgcga tgatgtttcc
    22861 taagtttttc ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc
    22921 agtaccagct tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg
    22981 ttcattcaga taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt
    23041 tgtgatcatc gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg
    23101 gttcacaccg atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc
    23161 gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta
    23221 gtagttacct agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt
    23281 caagatgttt tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa
    23341 tggaacgtgg acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag
    23401 tggatgcaaa cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag
    23461 tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa
    23521 aacatcccat acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga
    23581 agctagttcg cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat
    23641 ttcttttgga agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg
    23701 acctgagtag tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt
    23761 caacatatga gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg
    23821 acgaccgtta tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac
    23881 gatgattcca ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac
    23941 acgacggtaa agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat
    24001 tgggcgtaaa tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt
    24061 tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg
    24121 tccttgtgca gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt
    24181 atcaagtaat tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg
    24241 ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt
    24301 tcctggttcg atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct
    24361 catgtctaat acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac
    24421 aggagctgct aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc
    24481 cactccgcat cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc
    24541 acattcccag tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg
    24601 ttttaaggta cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct
    24661 gattttttcg ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa
    24721 aaggggttcc ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct
    24781 tgcagctcat agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac
    24841 atcgtctttt tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg
    24901 ctgttcggca aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat
    24961 gtcgcgaagt tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga
    25021 ttgcaattct ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc
    25081 acccttgacg attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt
    25141 caagatttct tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc
    25201 accaaaacgt tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga
    25261 gtaaggtccg attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat
    25321 gtacatcaca ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag
    25381 aacagttttc gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc
    25441 acttgcacca tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc
    25501 caagtgtaat tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag
    25561 catgatatcg attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg
    25621 ggatacaacc cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg
    25681 tttttgaacg atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc
    25741 ttcacgagta aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa
    25801 tgatgtatcg cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc
    25861 tgcagataat tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg
    25921 gacttctgca ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc
    25981 gacattaggg atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga
    26041 ttcatattct tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat
    26101 gatgatcgca tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg
    26161 gcctagagcc atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc
    26221 gactttctca cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg
    26281 acggaatttt gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc
    26341 agaagcatca acgtattcaa cgacaccgtc atgtttacat aacaaagcag ctcctgagtc
    26401 atgcgctgat ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg
    26461 cactgcttga cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa
    26521 gaaaggaata catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc
    26581 tactttatca atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg
    26641 agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc
    26701 gatatcagct gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg
    26761 acgataaggt gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt
    26821 gatcaacccg atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga
    26881 gtagtgaacg tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa
    26941 ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg
    27001 tgacaactga gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat
    27061 caattgttga ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg
    27121 ttccatacgt gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat
    27181 acgacgattt cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa
    27241 gaaatagctc attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg
    27301 atagccgttt ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat
    27361 gacttggaca gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga
    27421 atttaggcca ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt
    27481 tcctttttca acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag
    27541 attcaataga cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc
    27601 taagtcgtaa cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc
    27661 tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct
    27721 tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc
    27781 gccgaagatt tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt
    27841 taaaggaatc ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc
    27901 catttctagc caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt
    27961 tttgtcgact tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac
    28021 tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa
    28081 gaatacttct tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg
    28141 taatggtgca gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc
    28201 tttcaattca taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa
    28261 aatgtcttca aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg
    28321 aatttcgatc aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc
    28381 gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca
    28441 ttcaaaaaga ctacccgatc aattgttaca ataatatttc aaatattatt tttaggtaac
    28501 aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa
    28561 accttacttc gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt
    28621 tcgtttctgc cttttcttca tcagatagtt tttgcaactt catatactac acttattttc
    28681 tataaaagtc aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt
    28741 tcaattttat gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt
    28801 atactttaga aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg
    28861 caccaacaca actgaataac ttttacttta gctactttag ataggtttgt attcatcatg
    28921 atggatacaa acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca
    28981 gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt
    29041 atcaagcatt ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt
    29101 ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat
    29161 catcggatta ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc
    29221 cgcagaggca tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc
    29281 gttagatgct gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg
    29341 cgataatcta acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat
    29401 gagtgagaaa ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc
    29461 acaatcttta ttaaatgaaa caaccactat cccgatgact gtcacagcgg tcacagactt
    29521 ggaacaagcc catttagtca catccaatga aaaacctggc ggcaatgtga ctggcacgac
    29581 agatatggtg ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc cagacgccaa
    29641 aacaatcggc atcatgtata acaacggtga agcaaactct aagatccaag ctgatttggc
    29701 agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa
    29761 tgatgtccaa caagtgacga aaagtctagc taaagatgta gatggaatct atattcctac
    29821 tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac
    29881 acctgtcgtt gctggcgcag ttgatcaagt caaagacggc ggtctagcaa cagtaggaat
    29941 cgactacgaa agcttgggca aacaaacagg gatcatggca gcaaaaatct tagacggcaa
    30001 gaatccaagt gaacttcctg tcgaaacagc gaatgatttg gaactgtatg tcaataaaga
    30061 catggccaaa gcattaggca tcaatccaga cagcatcaaa gaagctgaat aaaacacagg
    30121 aggattctca tgacaaattt gctctctctt ttcttatcat ctgcatcgca aggggtctta
    30181 tgggccctac ttgcgattgg tgtatacgtc actttccgta tcttggacat tgccgacttg
    30241 actgctgaag gaagctttcc tttaggtgca gggatcgcgg cagtaagtat tacaaatggc
    30301 tatcatccat tgacagcttg tcttctaggc tttttagggg gtgcggcagc agggcttgtt
    30361 tccggactgc ttcacacaaa attgaaaatt cccgcccttc ttgcagggat catcaccatg
    30421 actggtttat attctgtcac atcccgtgtg atgggtgcgc cgaacatctc tttattgggt
    30481 cagccaacga tctttcattg ggtcgagtca ttcggcataa caaaagacaa cgctgtactg
    30541 atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca
    30601 gaaacaggac ttgctattcg tgcaactggc gataatctgg caatgagcga agcaaacggg
    30661 atcaacacag ataatatgaa gatcatcggt tatatgattt ctaatggatg cattgctctt
    30721 tctggctcct tacttgcgca aaacaacggc tttgctgatt taaattctgg tattggaacg
    30781 atcgtaattg gacttgcttc tattatcatc gcagaagttt tgttacgtaa ccagccacta
    30841 tttctccgtc tcttgactgt cgttttaggt gcggtcattt atcgttttat tttagcgtta
    30901 gtcttcgaac tgaatgttga accaagtgat tcgaaacttg cttctgcttt cgtgctagtg
    30961 atctgtcttt ctttcccgca gatccagcaa aaattccatt tacctcaaaa aaagaaaggc
    31021 ggtagtgtca aatgaatgac gtcttaacat tggaaaatct ccatcatacc tttgaaaaag
    31081 gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct ttccttagat cccggcgact
    31141 tcgtgacgat catcggtgga aatggggctg gtaaatctac tttgctgaac agcatcgccg
    31201 gtacattttc tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat
    31261 ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca agatcctaaa ctagggacag
    31321 ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg
    31381 gcttcttccg tggtgtcaaa ccacaagacc gaacattttt caaagaccag ctggttcggc
    31441 taaatttggg gttggaaaat cgtttatcct cagaaatcgg tttgctgtct ggaggacaac
    31501 gtcaagctat tacgttattg atggccacat tacagcgtcc agaactgatc ttattagatg
    31561 aacacactgc agcgcttgac cctgctactt ccaaaacggt gatggaattg acagaaaagc
    31621 tgatcactga gcagaatctc actgctttta tggtcactca tgacatggaa gacgcgatcc
    31681 gctatgggag tcgactgatc atgctgcatc aaggaaggat cgtcgtagat atccagcaag
    31741 aggaaaaaca gcagctcaca gtgccccaat tattagcgtt gttcaaacaa aacagcggca
    31801 cggcattaaa agacgatcaa gtacttttat cttcataatc agaaagagga tgggacaaaa
    31861 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
    31921 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
    31981 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
    32041 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
    32101 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
    32161 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
    32221 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
    32281 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
    32341 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
    32401 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
    32461 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
    32521 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
    32581 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
    32641 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
    32701 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
    32761 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
    32821 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
    32881 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
    32941 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
    33001 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
    33061 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
    33121 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
    33181 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
    33241 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
    33301 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
    33361 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
    33421 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
    33481 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
    33541 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
    33601 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
    33661 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
    33721 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
    33781 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
    33841 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
    33901 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
    33961 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
    34021 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
    34081 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
    34141 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
    34201 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
    34261 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
    34321 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
    34381 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
    34441 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
    34501 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
    34561 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
    34621 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
    34681 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
    34741 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
    34801 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
    34861 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
    34921 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
    34981 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
    35041 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
    35101 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catcaacttc
    35161 aagcgacagt acagatacta cctctaccac aagtgatagc acggatacat cagcttcaag
    35221 cgacagtaca gatactacct ctaccacaag tgatagcacg gacacatcag cttcaagtga
    35281 cagtacagat actacctcta ccacaagtga cagcacggac acgtcagctt caagcgacag
    35341 tacagatact acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac
    35401 agatactacc tctaccacaa gtgatagcac ggacacgtca gcttcaagcg acagtacgga
    35461 tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac
    35521 tacctctacc acaagtgata gctcagattc atctaccact acaagtgata gcacggacac
    35581 gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct cagattcatc
    35641 aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc atgcaagtgt
    35701 ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta ataaaacaag
    35761 caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc aatcgaataa
    35821 ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca cttttatgag
    35881 ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg
    35941 gacgttgctc tttatttttg ctttttatca taaatacaag aactacaatt caatttgtca
    36001 aaaaacacgt agatttcctt ctgagaaatt tatttacgta tcgaaccttt ccctattcag
    36061 tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt tcgaagagag
    36121 gtggcaggta atttctttat ttccttcaaa gccttttctg tgtaagaggc cgctaacttc
    36181 ctagcttctt ccacgcctcc tgtatgttca acgattttgc gtacggcgtc gacatcttct
    36241 tctgtcattg ccgctttctt ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa
    36301 gagtagatca acggtaaaga atagacccct tgacgcatgt cttctagtac aggtttgccg
    36361 atttcctggc tctcttttgt ataatctaaa atatcatcga taatctgaaa ggccatcccg
    36421 atagaaagtc cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa
    36481 gcgccaacag aacaacttaa ttggaacagt tcagctgtct tacctgaaat attttccaaa
    36541 tattctttta cagaaagttc ataattatag cgatcatcca tctgccctaa ttcgccattc
    36601 aatacttttt ccatactgcg agaattcaat tggagacttt tcaaggaagt ggagtaatca
    36661 gacaatagtt taaaacagca gacaaataaa tagtctcctg catagacaga aacgacatta
    36721 ccaaactgac tacggatatt aggctgacca cgtcgcagat ccgcttcatc aacgatatca
    36781 tcatggatca gtgtggccgt atggagcatt tcgatagaag ctgccaaagc gatggctttg
    36841 tctcgatctt gctctggtcc aaattgtgag aacaatagct gataggcagg acgcaacagc
    36901 tttccgccgg catggatcgt ttctttgatg gcttgttcga caggtttgtt tttcaggcgg
    36961 acagtttctt ccatccgttt tagtgtttct gtcaattctt tacttaaagc aggatagttt
    37021 tcccacattg gatgtagctt cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa
    37081 cattgggctc catttttgtt tggttccctg ctcataatct ttttggaatc gttgaagtgt
    37141 ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt gcaatccctg ataaaatccc
    37201 taaaactgaa aggactggca agtacaacat gactgtccat gattgtgcaa tgaaagaagc
    37261 catcaaaagc tgtccgacat tatgcatgaa accgccggcc gcactaattc cgatgatact
    37321 gacacgtttt ggtcctaatt gcttgaccaa taacattcca aaataactaa gcaaagcacc
    37381 actcatacta taaaggaatg tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg
    37441 tagccaaagt agtaagaagc tgtcttgttt tttcatggta aaaatggcaa cgatcgtgat
    37501 tagattggct aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt
    37561 ttccaataaa ccaatgatca ctccttgtgc aacaagtagc gaaatataaa tcattttttg
    37621 taatttactc atggtgtgtg aaggtctaat aaatcagact gtcttcgtca cctccatctg
    37681 ttgaacggac ttcgatcact aatttatgtg gcaaacagac gatggtttcg ccatttttcg
    37741 ttgcccagcc tcgacgtacg cagatctggt catcgcaatc tgcttctttt atccgtactc
    37801 gatcgccatc tatctcgatc aagttataat ctccgtgttc atctttgtaa gtatatgtgt
    37861 tgctctcttc tccagccatc aaagggaatg ttttgatttc tgtgccatcc acacgcagta
    37921 ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa
    37981 aagaaagaac agtcaaaagc aaaataataa aaacatccca aggtttgagc agactttttt
    38041 cagcaaattc tttaaggctc atttttgttc tcctttttcc agattaactc acccaatttt
    38101 agcataaaaa caagaggttg tgacacctgt gtcttgcttt gagtaataag aacgaagaat
    38161 agaaaacagc tttccatgtt ttctaattct ttgggcttat taccgagaag cagcaggtgg
    38221 aacaccgttt ataacaagag gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa
    38281 tttacgaaaa ttacttttca agtttttgtg aaatttcggc ttatttttcg aagaagctac
    38341 ttctggaaca ccgtttataa caagaggttg taacagaggt tgtgacacct gtgtcttgct
    38401 catggaggct tatctcgggc atcttattcc tttaatttta cataaaaagg aggctgtgac
    38461 ggtttgtctc agcccctttc tattgaaatc ttgcattcaa ataacgttct ttatctattt
    38521 atttcttgcc gtactgggaa cttggtctac cgtaccacca attacctagt gtacgttgta
    38581 gccatggaat tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga
    38641 aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat gcctgataag cagaacgtga
    38701 tcaccaatac aatcgttgtc gcactgcata gccatgtgaa taatccagca atcaatgcta
    38761 atgccaaggc aatttcaaag aataccatga atttttggaa gaacattgct acatcattgt
    38821 ttggcatgat cacttgcatg atctttgtga accagtctgg tgacttctca atcacagcca
    38881 ttggttcttc accgtatgca taactcaaac caaaatgtgc agcttgagtc gctgcttctc
    38941 cggcacttgc ccctgctccg ctggcagctg tggttgcatc accagcagca gaagcaccac
    39001 tggcaacatc tgttacattg tattcccatg tccaagggaa cacactgttt ccagtgaacc
    39061 atgatccttc accaaaccaa gtacttggtt tgaacacttc accattccca atgatttttt
    39121 tggaagcttc aacgaaccaa actaaaccat agaaaatacg aagtggcaca ctccataaga
    39181 cattgccgta acgagaagaa tgtcctcgag tcacgttgcg atcatctttg atatggaaaa
    39241 actcatgcat taaatattgg aacatataat aaccagaacg aatatcaaag aagtatttca
    39301 aattcacgat atgtttcatt acgatagcta aaaatccgct taaatggatc ttatcaaata
    39361 ggttagctac cccccatttg gcaccaatag aaaccataaa tccttgatag tttcctttaa
    39421 atttatgctt ttctccgcct ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg
    39481 tttgttcagc agcttgaacg atctgcggtg tcggtgttcc tgggaattct tcataaaaga
    39541 ctaaatcacc aatgatatag atgtttttat cttcgtagcc ttttgcctgc atatattcat
    39601 tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc
    39661 ctttgacacc agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt
    39721 tgatatgatc agcagccact tctacgattg gtgcattcaa taataattcc acgttgtgct
    39781 tacgcaaata gcgttcagct tttgcagcat catttcgatc caacatattc aagatcgtcg
    39841 gcattgcctc tacgaccatc agtgtgattt cgtttggatc taatttataa tctttggcta
    39901 ggcgattttt ccagtcgatc agttccccaa tcatttcaat accggtaaat ccagaaccac
    39961 agacaacgaa agtcagcatg gctttacgca gttcatcatc tggttccaat gcagcttttg
    40021 ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac caaagggtga
    40081 atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg ctctccgccc attccaagga
    40141 tcagctgatc aaaaggatag gaacctaatt tggtcttgac gatttttcgt tctttatcga
    40201 taccgattac agtgtctgtg acaagttgta cattcttttg acgagcaaac aaatgctgca
    40261 gatcgtattg gattgcttct ggttctacac gtccgccagc tacttcatgc aactcggtca
    40321 tcatagtatg gtacgaatga cgatcgatca gagtaattgc tacatcttct tttttcagtt
    40381 tctttgccaa gtattttgta gctgaaacac cggcatatcc tgctcctaca acgacaatat
    40441 tttgctttgc cattgatcat ccgctcctta tagactatct atataatgaa tatatgataa
    40501 ttcttttagt ttgatactta ttacacaatc tccatatatt atatagatgt tctgttcttt
    40561 tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt cacgaaattt
    40621 gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt tgacttatag
    40681 gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta gaaatggaat
    40741 ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt
    40801 cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac tcttcttctg
    40861 aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct gcaaaagttg
    40921 tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat tattcaaatg
    40981 gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa tcaaaatatg
    41041 ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg
    41101 aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac caattcttaa
    41161 aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca tctgaaagct
    41221 tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca gatacaatcg
    41281 aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa aagaactact
    41341 ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta actgaatcaa
    41401 actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa tataatgaaa
    41461 atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg aacgacgaat
    41521 tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt gttacaggtg
    41581 ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat gcagctgaaa
    41641 aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa tcacataatc
    41701 atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc tcacctttca
    41761 aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca cactgggcat
    41821 ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc
    41881 agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt tagggactta
    41941 cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg
    42001 agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt
    42061 gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca cgttagtcaa
    42121 gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga ttggtgcaat
    42181 cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag aagaaatcga
    42241 tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag aaaaaactgt
    42301 ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag gttacattac
    42361 ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg ttgacttggg
    42421 cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact ggacagtcgg
    42481 catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag aacggaacaa
    42541 aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca aaaaatacca
    42601 tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat
    42661 cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt caatgggtgt
    42721 gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag cgatcttcgt
    42781 gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca aattatcgga
    42841 ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac gaaccatgac
    42901 actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg tatttccttt
    42961 tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc taaacacttt
    43021 tttatttttt attggaatgc tagtttttga tttagcgact accgctatca ataattacat
    43081 ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca ttggccgcag
    43141 aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat ttgtttttgt
    43201 tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg gatttatctg
    43261 ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa tgcctttagg
    43321 ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg tttatgtcaa
    43381 tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac aatttgattt
    43441 ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct tggtgtttac
    43501 gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata ttgaaaatca
    43561 tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat tccagctatt
    43621 gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct atgaatggcc
    43681 gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc aattcaagca
    43741 aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct tattcaatgc
    43801 cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac agcatgaaga
    43861 ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa atagctcctt
    43921 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatca
    43981 ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt
    44041 tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
    44101 cagaggttgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggct
    44161 ttccacattt ttgcataaat ttggcttaat accgagaaac tggcttttga acaccgttta
    44221 tcagaggttg taaaatcagt gttttgtcct gagtattaga gaaacaaatg gaaaaatagt
    44281 tcctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga acactatttg
    44341 ttcggaaaaa gtgactaact atgacacaat ttttttcaca gtcccttttt tcacttgatt
    44401 tgattcgtac cagcgaactt gacgagaact ttcttggttt ggtaactata tggtattctt
    44461 ttaacatcct cgtttgcttc ttctaattta tttatacaaa gtcacacttt tttaaacatc
    44521 tgttcttcga tacatttcta tttcttttaa acatcgatac caatattttg aacttaagag
    44581 tgcataccaa atggattcat ttactcttcg tcagttacga agataatcaa aatctccaac
    44641 ttccctactt atttcgtttt gagaattatc gaagcaatct tacataaata actaaaaaac
    44701 aaaaaaccag aaaatcagta tactgatttt ctggttttac atggtttgtt caataaaaag
    44761 ctgttggaac tgttaatatt aaattatcgc atcgttacga attcttccgc tcctgttgga
    44821 tggatggcta ctgtattgtc aaagtcttct ttagttgcac ccatcttgat agccacggca
    44881 aaaccttgca gcatctcatc gacaccaaca ccaatagcat gtaagccgac gattctttct
    44941 tcttttccga cacagatcaa tttcatttcg catttttggc gataatcatt taatgcaaag
    45001 tacattgggg taaagcgtga acgataaact ttcaattcat tttcgccata tttttccaat
    45061 gcttcttctt ctgtcaaacc aattgttgca attggtggat gcgtaaagac gactgtcgga
    45121 acaagtttat agtcaagata agaatctttc tttccattaa ataaacgttc tgataaacgt
    45181 ctaccagctg cgattgcaac aggagtcaga tctaatttac cgatcacatc accaacagca
    45241 aaaatatgtt catccgttgt attttggtat ttatctactt tgataaaccc ttttttgtcc
    45301 aattccacat ttgtattttc cagacctaat ttttcggtat tcggtacacg tccaccggca
    45361 aaaagtacac aatcaccagt taaggtttca ccattttcaa atagcaccgt atattcgtca
    45421 ttgtttttag taatttcttt agcaatatga tgtgcgtaag tttttattcc agcttcttgg
    45481 tacatttgga tcaaattgtc cgaaagcatt ttatcaaacg ttctaagtgg tcgttctttt
    45541 cgatatgccc acatgacttc actacctaaa ccacgaaata cacctgaaag ttcagccgca
    45601 atgtatccag cacctaaaac gattgctctc ttaggtaact ctgttaattc aaaaaatcca
    45661 tttgaatcaa ttgcgtattc tccaccagga atggtcattt tacttgcacg gcctccagta
    45721 gcgatcaaga ttttaggagc acgataggtt tcaccgttta cttcgatcgt ttgactatcg
    45781 ataaaagtag cgtaaccttt tatagcctcg acatgattgc tatctaatcc tctttgataa
    45841 gcaccgtgta gaaaatcaat atatttttct cgtctttcta ctagaccggc gaaatcaaca
    45901 ttttttaaat ctgcttggat accgtaactt gaggcatctc gttcaattgt ttccaaaatc
    45961 gtacttgctt gccacataac ttttttaggt acacaaccga cgttgacaca tgttccgccc
    46021 aattcattcg cttcaatcag caaaactttt gcgccatgca tccctgctct gtttgctgaa
    46081 gcaataccgc cagagccgcc accaattacg atgtaatcat atatttgcat tttgttcctc
    46141 cttattgaag agatcaatca aaacctttat ttaacgatcc tttttctaca gaaaacaagt
    46201 gtaacacttt tcttgaattt tgtatgcgaa gatgcttaaa acagggatat atcagctttt
    46261 tccttattta cgttttcgga tcatatctcc aggtttcacc ggctgtgcga ctggcatagt
    46321 taaaatcatc attggatttg gtgctcggtc aatggattcg ccttcttcat tatgcataac
    46381 ttcaacggtt tgactgaaat gactgaatcc tggaccataa aattcgattt catctcctac
    46441 agaaaacaag tttctctgac gtatcgtagc aatctgtgtt gcctcatcat aagataatac
    46501 ttcaccgaca aatttatata ctgggatttt acgacgttca ccgaataatt gttcgttttc
    46561 agttggtgta ttgtagtaaa aacctgtagc taattctcgt tgtgctactt tccataactc
    46621 gtcgatccat tcttgcttgc agacatagtt ttcaggatct tccatataac tgtctacagc
    46681 cgctttatag acatttgaca ccgtagatac ataatggatc gatttcattc ttccttcgat
    46741 cttgaagctg tctacgccgt tttcaatcaa ttctggaata tgttcgatca ttgacatatc
    46801 cactgcactc atcgagaatt cttcttccac ttcgcctgta tcagttaaac tgtttcgttc
    46861 ttgaccaaaa ggcatatcga acaatccata cttccagcga caagattgag aacatccgcc
    46921 acggtttgca tcccgcatcg acatatggtt ggacaatgtg catcgaccag aataagagat
    46981 acacatggca ccatggataa atgcttcgat ttcggtatcc gtatttttac gaatctctgc
    47041 cacttcctcc attgaaactt cacgagctaa aacgacacgt tccaaccctt cttctttcca
    47101 gaattccaat gtctcaaaat tagttgctga agcttgagtg gataaatgga tcggcagacc
    47161 tggggcttct gtcgcacaaa tttcgatcaa agctggatca gaaacaatca ctgcagaaat
    47221 accgacatca cgcaaactgc ggaaaaattc ccctgctcca tcttcatttc cttcatgggt
    47281 gaccatatta gcagcaacgt ataccttcgc atcatgttct tttgcaaaag caactccttc
    47341 agccatttct tcataagtaa aattacctgc acggcttctt aaaccatatg cattcccgcc
    47401 aatatatact gcatctgctc cataatggat cgcggttttt aacttttcca gtgttcctgc
    47461 tggcgctagg atttctggtc gtttcaattt tctttttact gtcatttctt tcatcatact
    47521 ccttatttga tagcagaagg atcaatataa aagaatcctg tatctaattc acggttttca
    47581 ggatgcaatt tactgatttc gctagtcatt ttttccgctt tttcttgatc ccagctacct
    47641 tctaaaattt gtttttttgc ttcggcgaat agtcgagtga tgtttacgaa tttttcgcct
    47701 ggagtaaata atccgtccaa tttccatgta cagtaatgat gctgataaag ttcattcaat
    47761 tcattcgaga gattcagatc attgctggca aaaatatgtg tgccatgact atcttcataa
    47821 atagaataat gtgtctcttc ttttttaggt tccgagataa aaagacctct ttcacgatct
    47881 ttttgttcgt cttgtttcgt atagttatag taattctgta gcagtggacg tttggattga
    47941 tggatgcatg tagcaccata cacaagggtt tctacaggaa tatcgagttt ttcttccatc
    48001 gccttcatct cttcaaaagg aacttcacga gcaagtactg ctccaactgc gccttttttc
    48061 gcccagaaat tgatttgacg tgcgctcgtt actaatgttt cagcatcata aatatacggt
    48121 atacgcaatt caggattttt tttcatcgta taaataatac ctgtatcacc aactgtgatc
    48181 acatctacgt ttatttcttt taaaaaagcc aaatattcag gtacattttt cattttttca
    48241 ggatgaatga tcccatttac agcaatattc gcttttttac catacttgtg tgctaattct
    48301 accaattgtc gctgttcttc tcgtgtaaat gaagcaggca gacgcaatcc aaattgctct
    48361 tcaccgaaat atagtgtatc tgcacctgca gcaagcaact gttctgcttg ttcaatagtt
    48421 tctactgtgg ttatgagttc gatcataatt ttctcctcct acaacttcat aatagtagca
    48481 tagctctaaa aactttccaa tacttcttct atttaaagtc ggatttttga aaattatttc
    48541 acaaaaaatc aaaataaagt tcgatatatt ctgtccaaat ataaaaagag attgtgacaa
    48601 aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc tacacaggcc
    48661 aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca atgtgatctt
    48721 accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc caagcaaagc
    48781 ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac gtgcaccata
    48841 ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga ttttcacttg
    48901 aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga cgattgtatg
    48961 gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat tcaagaattc
    49021 aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt tgtgatcttt
    49081 agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg tcgctccgat
    49141 atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt tagaatctgt
    49201 taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat gtgctttctc
    49261 cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg tcaactgtcc
    49321 accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg tactgtattt
    49381 ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa acatgacttc
    49441 tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga acatgaatga
    49501 accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg cacgtgaaac
    49561 tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt ccagttctaa
    49621 caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc actccgatac
    49681 aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct gttttgcttc
    49741 aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc gtaattgagc
    49801 agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta gttcatcgat
    49861 ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac ggactttcgc
    49921 tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag tgatataacg
    49981 aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc catggtgctc
    50041 ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag ttggctcatc
    50101 tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat atttttgata
    50161 ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg ccggtttcaa
    50221 gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg tatgcaattc
    50281 atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct ttttcattcg
    50341 atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat caagcatcat
    50401 caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt gagctaaccc
    50461 ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt tattctttgt
    50521 ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa caactgggtc
    50581 cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta gcgtaggtgt
    50641 tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat tcattccttt
    50701 tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg cttttgaaag
    50761 gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt ttaataagct
    50821 catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt cgctgctagc
    50881 taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt tagggtaaga
    50941 acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg tttcattcaa
    51001 atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta aggctagtaa
    51061 aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt gtgcaatttc
    51121 taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact tcccatcttc
    51181 ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat cttcatattc
    51241 attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt ctttttttgt
    51301 aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct cactgattgt
    51361 atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa tccttacttt
    51421 tgcaattcgt atataaccac caccaccacg tttgctttct actagataac cacgttggat
    51481 ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa agaggtctgc
    51541 catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct ttatatatga
    51601 ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg actaatattg
    51661 accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg ttaaataaat
    51721 cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatcgacg cgcccaagag
    51781 gagtcgaacc cctaaccttt tgatccgtag tcaaacactc tatccagttg agctatgggc
    51841 gcatattcaa tttaaaaaaa ttataaatca tttgaaaaca aaaagcaaga taaaaaaaat
    51901 cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa accaagtgct ctaccaagct
    51961 gagctacttc ccgtttaaaa aataaaaaag ac
//
LOCUS       DAPBAB010000015        51340 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPBAB010000015 DAPBAB010000000
VERSION     DAPBAB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 51340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.015"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..208
                     /locus_tag="RD444_001079"
     CDS             <1..208
                     /locus_tag="RD444_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7548466.1"
                     /translation="QRPLNENSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRK
                     SLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(237..512)
                     /locus_tag="RD444_001080"
     CDS             complement(237..512)
                     /locus_tag="RD444_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548467.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            806..1621
                     /gene="thiD"
                     /locus_tag="RD444_001081"
     CDS             806..1621
                     /gene="thiD"
                     /locus_tag="RD444_001081"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDT7548468.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1826..3277)
                     /gene="cls"
                     /locus_tag="RD444_001082"
     CDS             complement(1826..3277)
                     /gene="cls"
                     /locus_tag="RD444_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDT7548469.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3619..6285
                     /locus_tag="RD444_001083"
     CDS             3619..6285
                     /locus_tag="RD444_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7548470.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6649..7401
                     /locus_tag="RD444_001084"
     CDS             6649..7401
                     /locus_tag="RD444_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7548471.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7423..8577)
                     /locus_tag="RD444_001085"
     CDS             complement(7423..8577)
                     /locus_tag="RD444_001085"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDT7548472.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8762..11203
                     /locus_tag="RD444_001086"
     CDS             8762..11203
                     /locus_tag="RD444_001086"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDT7548473.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11533..12237)
                     /locus_tag="RD444_001087"
     CDS             complement(11533..12237)
                     /locus_tag="RD444_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548474.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12424..12669)
                     /locus_tag="RD444_001088"
     CDS             complement(12424..12669)
                     /locus_tag="RD444_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548475.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12686..13537)
                     /locus_tag="RD444_001089"
     CDS             complement(12686..13537)
                     /locus_tag="RD444_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7548476.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13703..14707
                     /locus_tag="RD444_001090"
     CDS             13703..14707
                     /locus_tag="RD444_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDT7548477.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14948..15304)
                     /locus_tag="RD444_001091"
     CDS             complement(14948..15304)
                     /locus_tag="RD444_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7548478.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15672..16859
                     /gene="tuf"
                     /locus_tag="RD444_001092"
     CDS             15672..16859
                     /gene="tuf"
                     /locus_tag="RD444_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDT7548479.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(17047..19638)
                     /gene="mprF"
                     /locus_tag="RD444_001093"
     CDS             complement(17047..19638)
                     /gene="mprF"
                     /locus_tag="RD444_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDT7548480.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19664..20425)
                     /locus_tag="RD444_001094"
     CDS             complement(19664..20425)
                     /locus_tag="RD444_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7548481.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            20592..21224
                     /locus_tag="RD444_001095"
     CDS             20592..21224
                     /locus_tag="RD444_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDT7548482.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21283..22143
                     /locus_tag="RD444_001096"
     CDS             21283..22143
                     /locus_tag="RD444_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDT7548483.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22328..24643
                     /locus_tag="RD444_001097"
     CDS             22328..24643
                     /locus_tag="RD444_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7548484.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24825..26666
                     /locus_tag="RD444_001098"
     CDS             24825..26666
                     /locus_tag="RD444_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDT7548485.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26690..27799
                     /gene="rpoD"
                     /locus_tag="RD444_001099"
     CDS             26690..27799
                     /gene="rpoD"
                     /locus_tag="RD444_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDT7548486.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            28016..30157
                     /locus_tag="RD444_001100"
     CDS             28016..30157
                     /locus_tag="RD444_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDT7548487.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            30353..31216
                     /locus_tag="RD444_001101"
     CDS             30353..31216
                     /locus_tag="RD444_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDT7548488.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            31321..31794
                     /locus_tag="RD444_001102"
     CDS             31321..31794
                     /locus_tag="RD444_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7548489.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            31890..32777
                     /gene="xerD"
                     /locus_tag="RD444_001103"
     CDS             31890..32777
                     /gene="xerD"
                     /locus_tag="RD444_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDT7548490.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            32796..33167
                     /locus_tag="RD444_001104"
     CDS             32796..33167
                     /locus_tag="RD444_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDT7548491.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            33137..33934
                     /locus_tag="RD444_001105"
     CDS             33137..33934
                     /locus_tag="RD444_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDT7548492.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            33918..34487
                     /gene="scpB"
                     /locus_tag="RD444_001106"
     CDS             33918..34487
                     /gene="scpB"
                     /locus_tag="RD444_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDT7548493.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            34493..35209
                     /locus_tag="RD444_001107"
     CDS             34493..35209
                     /locus_tag="RD444_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDT7548494.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      35291..35415
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            35538..36149
                     /locus_tag="RD444_001108"
     CDS             35538..36149
                     /locus_tag="RD444_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7548495.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            36527..38050
                     /locus_tag="RD444_001109"
     CDS             36527..38050
                     /locus_tag="RD444_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDT7548496.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            38287..39627
                     /locus_tag="RD444_001110"
     CDS             38287..39627
                     /locus_tag="RD444_001110"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDT7548497.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            39781..40518
                     /locus_tag="RD444_001111"
     CDS             39781..40518
                     /locus_tag="RD444_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7548498.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(40683..40904)
                     /locus_tag="RD444_001112"
     CDS             complement(40683..40904)
                     /locus_tag="RD444_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDT7548499.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            40935..41966
                     /locus_tag="RD444_001113"
     CDS             40935..41966
                     /locus_tag="RD444_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548500.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            41953..43356
                     /locus_tag="RD444_001114"
     CDS             41953..43356
                     /locus_tag="RD444_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDT7548501.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            43422..44057
                     /locus_tag="RD444_001115"
     CDS             43422..44057
                     /locus_tag="RD444_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7548502.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            44106..44786
                     /locus_tag="RD444_001116"
     CDS             44106..44786
                     /locus_tag="RD444_001116"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDT7548503.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            44921..46150
                     /gene="rpsA"
                     /locus_tag="RD444_001117"
     CDS             44921..46150
                     /gene="rpsA"
                     /locus_tag="RD444_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDT7548504.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            46332..47642
                     /gene="der"
                     /locus_tag="RD444_001118"
     CDS             46332..47642
                     /gene="der"
                     /locus_tag="RD444_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDT7548505.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            47885..48160
                     /locus_tag="RD444_001119"
     CDS             47885..48160
                     /locus_tag="RD444_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDT7548506.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            48395..49441
                     /locus_tag="RD444_001120"
     CDS             48395..49441
                     /locus_tag="RD444_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548507.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            49443..50702
                     /locus_tag="RD444_001121"
     CDS             49443..50702
                     /locus_tag="RD444_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDT7548508.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            50708..51289
                     /locus_tag="RD444_001122"
     CDS             50708..51289
                     /locus_tag="RD444_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDT7548509.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
BASE COUNT        17147 a         8876 c        10793 g        14524 t
ORIGIN      
        1 tcaacgccca ttaaacgaga attctaacgg gattctgcgt cgtaatggac tgccgaaatc
       61 aatggatttt agaaaagtga atcagacatt tatttccagt gtcagcaatc aacgtaatca
      121 tattccaaga aaatcattga attacagaac accaatcgaa atatttttga gctatgtaca
      181 agaagcattt tattctagct taatttgaca aatcataaat aaagaacagt tagaagttaa
      241 aattgctgat tcacatagag tttatgactt cttggaaatt ctttttcaat cagttttttc
      301 atctgtttat gaaattcttt tttttcagta aacataaagg tttgggtatc gtaaccttct
      361 tcatgtagga aatagattat tcctttttct gtgttttgcg tataagcttt tatactcctg
      421 tcttttttga aagaacagat cgtatagcca tctatttggt ctggtagtcg tttgattttc
      481 ttcaaaatga cttttttatc ctcaagtttc atttttttca cctgacttta tttttttcat
      541 atggaaatca tagcgccttt ggttatatga aaacaagaag gatactgaaa tatatataaa
      601 tcaaatagtg gttatttatt attagaatag cgaatacaaa caaatgacaa ctattttctg
      661 atttattaca aatgtttttt caaataaatt tgattaccaa gaaaaggtag tggtatattt
      721 actgagggat actttgaaaa acatatgata gaaacagtaa cagcattatg aaaaatcttt
      781 ttataaaaat ggaggaatcg atttgatgga agatcagtat gtacaagcaa tgacgatcgc
      841 aggatcagat agcgatggaa gcgcagggat gcaagcagat atgcatacat tctttacaag
      901 aaatgtttac ggtgtttctg tcatgaccgc atgtgttgca ggtaattctt acggaatcgg
      961 agcaagtgtg acattgccaa cagattttat cgacaaagaa tttgaactga ttgcgaagga
     1021 ctttcaaata cgtgcagcta aaacaggtat gctagcagat tcaaagttga ttgagacagt
     1081 tgtaaaaaat taccaaaaat atgattttgg accattagtc gtggatcccg taatcgtaac
     1141 gaaacatgga aatcttttac tagaagaatc tgctttgcaa agcttgaaag aaaagcttgt
     1201 ccctttggcc gaagtactga cacctaattt ttatgaagca gaaaagctga ctggacggac
     1261 attagcagct gaagaagatt ttgtgcaagc agcaaaagaa ctgcaagata tgggtgcaaa
     1321 aaacgttatg atcaaaggac atcatgctaa agatgcgcag caagaaacag tcaatgacta
     1381 cgtattgctc gaatcaggaa aatcattttg gttaaatgca ccattttatc ctaccgaccg
     1441 gatcaacgga actggcgatt cgctatctgc atgtattgca gctgagttag cgaaaggaaa
     1501 aacagttgaa caagcgatcc gattggcaaa agactatgta aaccgtgcaa ttcgtgaaga
     1561 aataaatgta gggcacaaat atggacccat caatcattgg gcagctggtg aattagagta
     1621 aaagaaagag gtcgtgagcc tgccgtccag ctccgagtca caaggaacaa gttcaaaaaa
     1681 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca ggagaacacc
     1741 gtttgttcgg aagaagggac tgtgacaaga tttttgtcac agtcccttta atcttagtat
     1801 atttttctct acttgaataa aaaaattata ggattggaga aaataatcga gaaatttgtt
     1861 gcttgaataa tagccagtta gacatttcct tgactgtttc agttgtcatg gatgaacaat
     1921 gtgtctcatc ttctttgaac tgagtagcaa gttcttctaa aaactcagga tcgtaaatga
     1981 cagcacttgt ttcaaaattc aatcggtagc tacgaatatc ttgatttgct gaacctacca
     2041 tgcagatttc atcatccatt atcaatgttt ttgcatggag gaagccgttc tcatagacaa
     2101 ggatttgtac attttccttg atcagctgcc gagcataata ttgtgttgct cgataaataa
     2161 aaggatgatc cggtttgttg ggaatcatga ttttcacatc tacaccagag gcagttgcga
     2221 tttttaaagc agcaatgaca ctttcatcag gaacaaggta aggtgtctgt atccaaacac
     2281 gttttttagc agaagtaatc aatttgataa atgaaagctt gatttgttcc ctttcgttat
     2341 tcggtccgct tgaaacaagc tggatggatg tatcagcaag cttagattca tctcgatctg
     2401 cttttttaaa gaaataattc aaatgatagc cgaccttttt ttcttcaggt accgagacgt
     2461 tccagtctgt taagaagcga agctggagca aagaagaagc ggcaccgaaa atccgtatat
     2521 gcgtatcgcg ccaatagccg aatttttttg ttgtatttac atattgattg gcaatattga
     2581 aaccaccggt ataactaatc tttccatcga tcacaacgat ttttcggtga tcatggtaat
     2641 tcaatcggaa acgaagtaat gccttttgtg aggtaacaaa tgtatggaca tgtccaccat
     2701 ttttgattag acgattgaaa tcttttgctt ttgtaccatg agagccaaat gcatcatata
     2761 atattcgaac ttccacgcct tctgcggctt tttcttctaa aacatgtaag acttgctggc
     2821 cgatattatc tgtcacaaat gcgtaatatt cgatatggat cgaatgtttg gcttttttta
     2881 gatcttgaag cagtgaatcc aatttctctt gtccgtctgt gagaagagtg acagaattca
     2941 ttcttgtcag cggcatacga tttaatgaag agaagaagtc gacaaattgc tgtttgtctt
     3001 tatcgcccat gtcaggatcg taatgttcaa tggtatctcc tctaaaagac tgaaagtttt
     3061 ctaattcttt caagtcactt tgtcggagat agaatttttt cttatccgtt aatccacgtc
     3121 cgaaaaataa atataataca aagcccaccc caggaagggc aaacaatact aagagccatg
     3181 cccaaatggc tgctacatct cggggtttga tcaaaatagc caccaaagca ataagtgcat
     3241 ttaatagata aagggcggtt ataatactag ataccacaaa aatgttcctc cttacataca
     3301 ataacattag tatagcatga tagaagaaag aagcctacca aggtgaatat acttttcact
     3361 cctttcatga aaatgtaggt aaatcgggct aaagtagttg ctttttgtag gttaataagg
     3421 taggatatta gtacagttga atatctcctt aggggagtaa ccggcaacgt ttgttgcgat
     3481 aatatcaaca gtaagttaac caaatggttt tctggtattg tatgaaatgg tgagacctaa
     3541 gacaaatgcg tatttgtctt aggtctcttt ttttttcaga caggactagg gccaagatca
     3601 aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac aacaagcggt ctataaagaa
     3661 acgatcgact cgttgatgca gcaaacgcag agctcaaccg atggattgag caccacgcaa
     3721 gcgaaggaaa aattagaaag agatggcttg aaccagctaa aagaaaagcc gaagaagcca
     3781 acttggaaat tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt gctgattgtc
     3841 gcactgattc agatggtcat gggtgactac atcgaattct tagttatttt tgcagtattg
     3901 atgttgaatt ctataatcag tgttgtccaa acaaaaaaag cagaaggttc tttagaagcg
     3961 ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg atggtaagga aaaaacgatt
     4021 ccggcaaacg aacttgtggt aggagatatc gtgttgttag acgcaggcga ttatgtacct
     4081 gctgatggac gtttactaga tgcgggatca ttaaaagtag atgaaggtat gctgacagga
     4141 gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc aatcggagat
     4201 cgtacaaata tggtattcag tggaacgatc gtaacttacg gacgcggtaa attcttagtg
     4261 acttcaactg ggaataatac ggagatcgga caagttgctg gccttttgga atcaacaact
     4321 gagcaagtca ctccgttaca acgaggtctg gataaattca gtaaaaaact aagtctggct
     4381 attttggcat tgtctttatt gattttaggt atccaactgt ttcgtattta tcttggagaa
     4441 ggtacaggag atatgactgc agatatcgtc agcgcagtga tgtttgccgt agccgtagcc
     4501 gtagctgcta ttccagaagc attacagtct atcgtaacaa tcgtgttatc tttgggaacg
     4561 aataaaatgg caaaacgtca tgccattatt cggaagcttc cagcagttga aacacttggt
     4621 tcgacaagta tcatctgtac agataagact ggtacattga cacaaaacaa aatgacagtt
     4681 gttgactact ttttaggtaa tggcgatact ggggatttca caaatgaccc ttcaaaatgg
     4741 acagctgacg aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc atcgatcagt
     4801 gaagacggta ctaaattggg cgaccctacg gaagtagcgt ttatcgactt tagtgaaaaa
     4861 ttaaaccagc cttatcagga aatccgaaac aactacccaa gacaagcaga acttcctttc
     4921 gattctgatc gaaaattaat gtctactgga cacacgatcg ataacgaaaa ttacttgttt
     4981 gtcaaaggcg gaccagatat cgtcttccaa cgcagtaaaa aagtattgct aaatggtgaa
     5041 gtcgttccga tgacggatga attgatgaaa aaattccaag agcaaaatga agctttttca
     5101 acacgcgcat tacgtgtact agcttttgct taccgtccac ttgataattt tgatttatca
     5161 tttgaagatg aagacgattt gatccttgtt ggtttgcttg ctatgatcga tcctccacgt
     5221 gctgaagtga ctcaggcggt aacagaagca agaagtgcag gaatcaaaac aatcatgatt
     5281 actggtgacc acaaaacaac agctgttgct atcgcaaaac aaatcgggat ttttgaagaa
     5341 ggaaacctgg ctttgaccgg gacggaacta gatgaattga cagaagatca actaaacgaa
     5401 gttttagaaa aagtcactgt ttatgcacgt gtttcacctg agaataaaat tcgtatcgtt
     5461 cgtgcttggc aaaataaagg tcatatctca gctatgacag gtgatggtgt aaatgacgca
     5521 ccagcattga aacaagcaga catcggaatt gctatgggaa cgggtacaga tgtggcaaaa
     5581 gatgcagcag cgatggtatt gacggatgat aactttgctt ctattgtcag cgcagtagaa
     5641 gtaggacgta atgtgtttga taatatcaag aaagctattt cttatctatt tgcaggaaat
     5701 ttaggtgcga tcattgcgat catctttgcc ttagttgctg actggtccaa tccatttaca
     5761 gcattacaac tgttgttcat caatttagtc aatgattcac tgccagcaat tgcattaggt
     5821 acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa ttcaggaatc
     5881 tttacggaca aaacattgat ttctgtaacg tatcgtggtg tgctgattgg gattgctgtt
     5941 atctttgctc aatggcttgg catgcagcat tcacctgaat tcggtgtagc catgtcattt
     6001 tcaacattga tttggtgtcg gacattgcaa acattcccag cacgttcaaa cacacaaaca
     6061 gctatccaag cagggttctt ctctaacaga agtgtcttac tagctgtagt tgtatgtagc
     6121 gcgctttact gcttaacaat gatcccagga ctgaaagaag tcttctctat cccatcagca
     6181 tttggattga cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat tgttttgatg
     6241 gaaatcacta aattgatcat tgtacgagta caaggaaata aataaaacga atcaacgagg
     6301 ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac agcttctcat
     6361 gtttgttgaa tattgtgact tgtgaccgaa gagttggcag gagaacaccg tttaaggaag
     6421 aagttgtaaa atcagcattt tgagctaata ctgagggtca tctgattgga cactgtttat
     6481 tcggaaagag ggactttgac acgactttgg tcacagtccc tttttttatt tatgatagac
     6541 ggaaagccaa tcattttatt attcatcaaa agagatagaa tagataaaag aggagaataa
     6601 tttattccga aaagtgaaaa catataacaa aaaattagag gaaatcaaat gaatgaaaaa
     6661 gaatgggatg aatttgctga agaatattat gaaatccagc aagaatcgca agtaactatt
     6721 gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga aacagcagca
     6781 gacgttgctg gaggatctgg cagatttctt tccttagcaa aagaaattaa tcgctatgat
     6841 ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga aaaaaatgag
     6901 ttgccaaatg tttcctttat caaacagtcg ttagaagaat ttatccaatc aaatgagaca
     6961 tatgaaatgg tttttacagc agcaaatcca gcattggtac atcttattca attagaaaaa
     7021 ttgatgaaaa agtcaagcaa atggtgcgtg ataatcaggg tagtggaatc aatcgatgat
     7081 gttttttatc ctatcgaaaa gcaactagga atgatagaag aagatcctaa tacatcgcca
     7141 gttgtaatgg atcaatttga aacatactta attgatgaag gattcaaagt gaagaaaaaa
     7201 gattttactt attttacaca ggaatcaatt actgaagaat tgatgaagct atactatgac
     7261 gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca aactattcag tgaaaaacga
     7321 gaaatcctta gtacgactaa gctgacctat agactgctcc tttggtccgc agataaaaaa
     7381 agaacctttc acgactcgtg aagggttctt tttatgattt tattatattt tgtagtaacg
     7441 aatatcattt tcgatctttg taatcgaaaa aggtacatct gaagtatatt ctgcacattc
     7501 tccgtgttca ggaagcgtct cactgaaaat cgtttcatat tcgtcaacag tgatccgtgt
     7561 tcgagaatct agcatctctt gatgagattg tgcgaagaga tattcttgat agttttcttc
     7621 cagataacct gtaaaaaatt cacttactgc accagaaccg tagctgaata gaccgatgcg
     7681 atctcctggc tgtagtgatt tcgaattttc cagtagagaa gttagcccca ggtataatga
     7741 accagtgtaa agattaccga ttcgtcggct gtaacggatg ctttcttcat agcgagccat
     7801 aagacgttcc tgattttctt cgtctgtttg gtctaatacg ctttgcaatg ccttttttcc
     7861 catcttagta tacggaatat ggaaagcaat cgcctgataa tcttcgagtc ctcgacccga
     7921 caattcttta tgtcgattcc aaactttttg gaatgattcg atatacgtag aattagataa
     7981 aggaccatca acaacaggaa attcgctata atctggacgc cagaaatcat agatatcttc
     8041 tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc gggttttgag tgatcatcat
     8101 cgcaacagca ccgacacctt gcgtcacttc accaccgctt gccaagccgt aacgagcaat
     8161 atcacttgct atgactagta cttttcgttc tggatgattt ttgacatatt ctttcgccat
     8221 gtgcaaagct gcggtgccac cgtagcaagc ctcttttact tcaaaagaac gagcaaaaga
     8281 ttggattttc agtaaatggt ggataattac cgcactcgct tttgaatgat caatcccgga
     8341 ttctgttccg acgatgacca tgtcgattag ctcacggtct ttttctgtta cgattttgct
     8401 ggcagcattt gctcctagtg taatgatgtc ttcatttgca cgattgactg ccatttggtc
     8461 ttgaccaatc cctatatggt attttgcagg atcatcccca cggctttctg ccaactctgt
     8521 catatctaaa tataaattag gaataaaaaa ggaaagacga tcaatcccta ttttcatttc
     8581 aacacctctt tacttatgat aaaaaactta ctttactaag gtatcataaa aagattaaaa
     8641 gaaatataaa aaagagaata tttaaggatt gttaaaattt aatcttagaa aaaaacaact
     8701 ggaacaagag ctatttgttt ttacagcaaa gaatggtata tttttgtagg aggtggctta
     8761 cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt atcgcggaag
     8821 tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat tattagataa
     8881 aacaaaactg aaaaaagata aaatcgatca agtaatattc ggaaatgtcc tgcaagctgg
     8941 taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg tagatgtccc
     9001 agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct tagcacgcca
     9061 gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga gtatgtctca
     9121 agcacctatg ctgaaaccgt atcagtcaga aacaaatgaa tatggtgaac caatttccag
     9181 tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat taaccgcaga
     9241 gaaggttgca acacaatttt ctgtgagcag agaagaacag gatcgctatg ccttgtcgtc
     9301 ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt
     9361 caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa atagtacatt
     9421 ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa
     9481 tgcttcgcca ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga
     9541 aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg gtattgaccc
     9601 aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata gatcaggaat
     9661 gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt cttctatcgt
     9721 agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag gagcaatcgc
     9781 attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag cttacggatt
     9841 gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag gtttaggctt
     9901 ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa aaaaaaagtt
     9961 ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg tattgacaca
    10021 agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg atcacatgat
    10081 cgaaaaccaa gtgagtgaag tggaaatccc aatgggcatc gcgcagaatt ttcaaatcaa
    10141 tggtaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg cagctgcaag
    10201 taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg
    10261 gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag ttaatcatcg
    10321 aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga aacgcggggg
    10381 cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat acttatctat
    10441 tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact cgattcttga
    10501 gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat tcagtatttt
    10561 aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat tcgaacgctt
    10621 agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg caggggaata
    10681 tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga atggtataga
    10741 agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga ttcacgccta
    10801 cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag gggataaact
    10861 agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt ccaatatcct
    10921 gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg aactagcaca
    10981 agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat tagttacaga
    11041 aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct caattggggc
    11101 tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa agatgaacca
    11161 gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca atatctttag
    11221 aagaataaag caagatttac agttatttta tttactgcaa atcttgcttt tttctctatc
    11281 agaaaatatg tttattatta gaatttttta tagttaaggt taggcatctt aagaaatcag
    11341 gcaactccaa gatatcttca ggtagaagag aagtcgccta acattgtagg atcataggtg
    11401 atttgttgga caggtcaagc tgatatttga gcctgatgtt agaaggaatt tctttttact
    11461 tgctgtagaa attattatat gactaaaaag gctgggattt taaatcccag ccttttatga
    11521 caaatagaat ttttatcgat cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat
    11581 caaagtcaat ttgactttgt atttccgttc gagtgctttt tcgataacga actcattttc
    11641 agtagtcata gaaatagttg ctaatttacg aggttcatca gcattttttt cattaataac
    11701 tacttcatgt tcattttgtt ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa
    11761 atcagcaaaa cttaaagcgg cattcgtatc agttgagcgt gtattttcag ttgtgtccgc
    11821 ttttggtttt tccgtttttt cttctggcac actgactgtt tcttctgtaa ctggtgtcgc
    11881 aacagcttgc gcagttggtt catcgttttt caatcgttga tcattccagt aagacacgat
    11941 ttcaataaga agattgtcac ggaatcgttc gaaaatcccg ttttctcctg aacgaattct
    12001 ctttaataca gtattgaagt tgtcatcgat tggcacgttg atccgtgtat cttcatttgt
    12061 tttcccagct aatccgatcg ttaagatttg acggtttttg ggatcaagca tgacttgtaa
    12121 cggcaaacca tagcgtcgca agctctcttc ttctagagaa cggaataatt gtcctcttac
    12181 accatccaat gaaggacgga attgatttaa taatttttgt ttctgttttt tcttcatatg
    12241 ttctctcctt cgctcatacc ctttcgatta taagaaaaaa acagccaaat gaaaaggagt
    12301 aatgcaaaaa aacttgcgta tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt
    12361 ttttgaaaga aaaataaaaa atggaacgga ttatgtatat atacataatc cgttccattc
    12421 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
    12481 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
    12541 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
    12601 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
    12661 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
    12721 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
    12781 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
    12841 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
    12901 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
    12961 ggatcatatc atgttttttt gcatactcaa ctacagttgg ctgtaattcg cctggtgcaa
    13021 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
    13081 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
    13141 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
    13201 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
    13261 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
    13321 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacactttct tcattcttgt
    13381 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
    13441 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
    13501 catttaatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
    13561 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
    13621 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
    13681 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
    13741 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
    13801 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
    13861 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
    13921 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
    13981 acctagtgca gatgtatttg ttgttttgta tgaattagat acagtgatgt acttatcttt
    14041 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
    14101 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
    14161 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
    14221 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
    14281 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
    14341 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
    14401 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
    14461 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
    14521 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
    14581 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
    14641 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
    14701 tttataaaaa tgactagaac attaggtgtt caagtaccag tgatcagtta aatgaattgt
    14761 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
    14821 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
    14881 ttatttcttg gagttaaacg cttctgtccc agcctctttt tatctaataa aattttaaga
    14941 ccatttacta attatcgatt ttttctgccg catttttagg cacttcagaa gcctttattt
    15001 cttcccagct taatacacct ttacgaggat tgactttgag tttaagatac gcattcatct
    15061 ttaatggatg ttctctttct tcgcgaagcg tcacctgttt tttttcacca tctttattat
    15121 aagccgcttg cttgtaggta taagtcgtga atttctctcc gttatctgct tgggtagctg
    15181 acatttcgcc attcgtcgtg atttttgtat aataagcttc gccgccgtaa aaataatgat
    15241 aagctgcaaa acttccgccg agaaatatgc ctaaaacgac aagtacgcca attaattttt
    15301 tcatgtactt cacctctttc cttattatta acgaaataga agatttcgtc tattgaaagt
    15361 tgttggcaac agctgaattt taaacttttc ctcattttgt tcttattgct tcaaaagaaa
    15421 gttaaggact atcttgaagt ttttctcaaa aaacacattt ttttatttat aaaaggtact
    15481 tttttcgcgt ataagacaag cgagtgctct tagttgactt tgaccaatat tgacgtataa
    15541 taagcttagt tcgaatctta caaaaagtaa gtcggacgta ttctttttga tttagtcaaa
    15601 aaaagactat aataaaaagg tgagagctga aatttttcag tgatctatat tatttaggag
    15661 gtttgtaaat tatggcaaaa gaacattatg acagaagtaa accacacgtt aacattggta
    15721 cgattggaca cgttgaccat gggaaaacta ctttaactgc tgcgatcact acagtattag
    15781 gcaaaaaagg cctagcaaat cctcaagact atgctagtat tgatgcagct ccagaagaac
    15841 gtgaacgtgg gattacgatc aataccgcac acgtagaata tgaaacagaa aaacgccact
    15901 acgcgcatat tgatgcacca ggacacgcgg actatgtgaa aaacatgatt acaggggcag
    15961 cacaaatgga tggtgcaatc ttagttgttt ctgcaactga cggtccgatg cctcaaacac
    16021 gtgaacacat tttattgtca cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca
    16081 aagttgattt agtcgatgat gaagaattga tcgatttggt agaaatggaa gttcgcgagt
    16141 tattgagcga atatggtttc ccaggcgatg acactcctgt gatcaaaggt tccgcattaa
    16201 aagcattgca aggcgatcca gatgctgaag ctgctattat ggaattgatg gatacagtag
    16261 atgaatatat cccaacacca gaacgtgata cagataaacc attactattg ccagtggaag
    16321 acgtcttctc aattacaggt cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg
    16381 ttcgtgtcgg tgatgaggta gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg
    16441 taacaggtgt agaaatgttc cgtaaaacgt tagattacgg ggaagctggg gataacgtag
    16501 gcgtgttgtt acgggggatc caacgtgacg atatcgaacg tggacaagtg cttgctaaac
    16561 caggttccat tactccacat acaaaattca aagcagaagt gtacgtgttg acaaaagaag
    16621 aaggtggacg tcatactcca ttcttcaaca actatcgtcc acaattctac ttccgcacaa
    16681 ctgatgttac aggaacaatc acattgccag aagatacaga aatggtcatg cctggtgaca
    16741 acgtgacgat tgatgttgac ttgatccatc caatcgctgt tgaaaacgga acgacattct
    16801 caattcgtga aggcggacgg acagtgggtt caggtatcgt tacagagatc gaagcataag
    16861 aaacgcgact cgaatatttt ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca
    16921 ggatttctgt ttggaccgct ctttgttctt caattgtata agaagagatt gtagaagcat
    16981 gaactttcgt tttacttcag tctcttttat tcatcatgag agtgttcatc tatcggtatg
    17041 cagtttttaa tactttcttc gtatccattt tttcaaacca cttattctac cttgtgtctg
    17101 ctcaactggc gcattatcaa taatcagcaa agcaatcata acatatgcaa tccaactatc
    17161 tctagaatat agagtataac gtgagaccca gtttgtagcg tacttgcttt tatattctct
    17221 taatccatgg aaagaataaa aatgagaacc aaactcgtaa accaagtatg caatcctttc
    17281 ctgtaaaaaa ctctttctgg aagttccgac atttgaaaga ggggccatcc ctaaattgaa
    17341 ccattggata tgttcttctt tcatataggc aaacaaatgc aagaacaaga aatccatact
    17401 gccagaaggt gcttttttag gatcatatcg catcagatca atcgttccta ctttattgtt
    17461 cgtgtaagta ggcatgatcg ttgcaaaagc gacaatttct tcttgggtat ttttgacaac
    17521 tgcaataggc gctctttgta gataatcttc agagaaaaag cctaaagaaa accctttttc
    17581 ctttcgtgaa cccaaccaat tatcagagat atttttgaaa gtactcattt gctcagtaga
    17641 aaatggtggt tgtaagacat caaatgtaaa tccttctcgt tcgatccgat tcaagacagc
    17701 acgagtgccc ttcatttttt tccctgaagt tgtaaaacta ttcaggtcta cataagcttc
    17761 ttcccccatc ttaatgaaat cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct
    17821 tgtttcgtag aaaactggta aatagcatag tcgatctgtt tcttcgataa aagcttcgat
    17881 tgcttctgga aaatcttctt tcttaccaga aggatcaccc attacaatac atttattatt
    17941 gaagcaagcg aattggagaa gaacagttgg ttcaccgtct ttttcatagg caaacatccg
    18001 cttatctttt aagaagatca attgactatc ggagtttcct ccataagttg taaggatctt
    18061 cagggccttt tcttcattaa aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg
    18121 aacaaataga acgatcatga aactaacagc gatgatggcc aacaatcccg aaaaccaaat
    18181 cttttcagaa ggaaataaga aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg
    18241 cagattgtat accccaataa cgatgtaaag caaggacagt gcaccaataa tgattccatc
    18301 gatcgtacgc cactcccatg aatagatcaa ttgttcccga aataattcgt ttttagaagc
    18361 aagaatgatc agaagcagaa tagacaagaa aaccaccggt gtgaaactaa aatcacttaa
    18421 taaaacataa agtaaagcta gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac
    18481 acgtgctgcg atccctctgc ccataatgat taaaagaaat cccaatagta tactaggaaa
    18541 ttggataata aaatgaaatt tcaatgggtt caaactatga agccagcgaa actctgtaaa
    18601 agcttgcggg atagtggcag aaagtactag catgattcca gaaaagtata ataagacaac
    18661 cacgatttta tgcgcaattt ctgttgctaa ttgtttaggg ataccagaat aacgctgatc
    18721 aaaagtggaa ccgatatttt tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta
    18781 gtagaacagg cgatagagca agatccacac aacgactatt tctcttggca caccaatagc
    18841 ggacaacccg ataatcatca ttacatcgaa actcccaatt tctcctggaa tcatagacac
    18901 gattcctatc actgaagcag caatgaataa cggaattgtt tgccacagat caatccggat
    18961 ttccattaaa taaccaacag aaaggaaact aaacaggaca ccggtccatt ccaacaatga
    19021 aactagcaat agctgtcctt gagttttaag tgaggtacca ccaaggaatc ctttcttttt
    19081 gaaagtagta aataaaaaga cgacaggaaa atatagaccg ccgccgatca accatatcca
    19141 atattgcttc aaaaaaggag caactggcgt gaagactaca agtaaaaaag aaagcagact
    19201 gtaaatagaa agtccagcca tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt
    19261 tctgctctct ttcttttgtc catatagttc agagcgaagc cccacactaa caaacccgcc
    19321 gaaaccggca atattgttga ttgtattgat cagccagctt gtctcaaaca agtaacgagg
    19381 attctgcttt tgttcaagca tccgattcaa aataatatca taaccaatca taggtaacac
    19441 agagacaaga ccaatcaaca gcataagtcc gcttttccat acaggtatag tggcaaacgt
    19501 ttctcctaat tgctggatag atagagtttt accgatggac atcaattcac cgacaataat
    19561 gacaatgaca gaaatcaaaa agattgtttt gaacagtcct aggtggtttt tcagccattg
    19621 tatggtattt tttaacaaat aaatcacctt ctattcctaa tagttatgtt tcagaaagaa
    19681 attgaagaac ttctcgattg aagcgttcag gatcttttct agctaattca tgtccttgtt
    19741 ctttcacaag cacaaaggaa gcttttggta tagttttagc tatatacaaa gaatgtttta
    19801 gcttgattac atcttttttt cccacgatga tcaaagtggg ggagttgatt ttttttaaat
    19861 cattttctgt aagcccgatg tcatgcagca aaagtttgat aactaaaaga tttttgcgaa
    19921 gacttgggcg aaataaagag agtaaccata cccatgcata atgaatatta ctgatgactc
    19981 tggcagaaaa acgaacgcct ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa
    20041 cttttttagg gaagctgctg gcaaatacca gtgctagatt tgctccatca ctaaatccaa
    20101 gaaaatctgc ctgatcgatt ttttccagca acatgatcgt attcagatcc tcggccatta
    20161 actgaaaatt caacatagat gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta
    20221 gatacacggt atagtagcgt tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc
    20281 cgtcattgcc atgcagtaaa aagagaggga agccttgacc gctcttttca tagtaaattt
    20341 cgctcccatc tggcattttt gcatatttct tttctttttt cacataatca cctactgtgt
    20401 aaagtataac gattatatca gtcattgggc aattgttttt actgaaaaac aacgatcatt
    20461 ttcgtaagat gacaaaaaat acaggaaaat tcattttttc ttaaggcgta cacagaaatc
    20521 aaggaagaaa tagccaagaa gataaagaaa tagtaagata aaaaaagatg tcaaaataac
    20581 taggtgatga aatgaagcga aaacgattat ctattatccg tattcttttt tacatgatca
    20641 ttctgacggt tattggtgcg gtgttgtttt taggaggaag aacttatcgt tcggtaaaac
    20701 gagtggaaag ctatcaaata gaaataacaa cagctgcaaa aaattatcat atggaaaact
    20761 acgagaattt gatcaaggcg ataatattga ccgaatcaaa aggaaaaggg acagatctca
    20821 tgcggagctc ggaaagtctg catggagaag ctgagatcat tgataatcca gctgacagta
    20881 ttgatcaagg tgtcaaatat ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg
    20941 atttatggac agctgtccaa gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa
    21001 atcatggcgg agaaaacaca gtagaattag cagaagcgta ttctaaagat gtgctgagcc
    21061 cacttttagg aaatgaggaa gggaaacaat accgatattg gcggatccag tccttgcttt
    21121 acaatggcgg ctatctctat cataatggag gtaacatgtt ttatcctgat attgtcaaaa
    21181 tgaatgaaaa aataatagag acctatgaaa aagtttttaa gtaaaatagc aggaaatgtt
    21241 caatcatgaa taaaattaaa tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa
    21301 atggaaaatc aaaaagtggg gtacacaacc aaagactgga tgttacggtt tgtgaaggga
    21361 atgtttatcg gttcaggatt tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc
    21421 tttggcatgt atgagcggct gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa
    21481 aatgttttgt tttttctgcc tgttggtcta ggcggagtga caggcatctt tctactttct
    21541 tttttagtca gttttttatt aggtgcgtat gaaacaacga tattatggtt ttttgtcgga
    21601 tgtatcgctg gaactgttcc tgcgttatgg aaagaagcag ggaaaaaagg aagagatact
    21661 tctgatgtga tcatcatggt tgttactttt gttctggctt atttgttttt attatacggc
    21721 gctcaattat ttgacgggca agtcgatcag aattttggaa catggctgat tgccggcgga
    21781 ttgattggtt tgggtatgat cgtcccagga ttgagccctt ctaatttttt ggtgtatatg
    21841 ggattgtata aagcgatggc tgacggattt aaagaagtac gacttgaagt gattgtgccg
    21901 atcggaattg gtgggattct ttgtgttctt ggattgtcaa aaatcatgga ttacattttc
    21961 tcaaaggcat atagcaagct attccatttc attttaggtg tagtttttgc ttcaacgatt
    22021 atgatcattc ctaccgatta cgcgggattg ggagttgctg gggtactagc ttgtcctgtt
    22081 ctttttatag ccggtacagc actaggttgg tggatgagcc gtttggagga acaatataag
    22141 taaagataga ttgataataa aaacagcaca cgggtgattc aaacaaaaag gttggcatct
    22201 tttttgtaaa atcattcgtg tgtttttcta taaattttat aaaaacaagt tgtttttaat
    22261 cttggaacac gctagactta aacacgtgat tatttttgaa agttggcgga aaatggagaa
    22321 aaagaaaatg aatggactta cacagcaaga ggtagttcag aaaatggctt ctggccagca
    22381 aaatgactac caggaagatg catcaaaaag tacaaaacaa atattcagcg ataatatatt
    22441 gacgttattc aattttctga actttggtat tgggatttgt ctgttatttg taggtgcgta
    22501 ttccaacatg gcgtatttag caattattgt cgtcaatatc gttattggaa tttatcaaga
    22561 aatacatgca agaaatcttg tacggaaatt atcgattgtt tcccaatcag aagtactagt
    22621 gatccgggac ggtaaagaac aatcgatttc tactaaagag ctggtattag gagatttggt
    22681 aaaaatcgga gcaggagaac agataccagc cgatttacag gttatatctg gacgggcaga
    22741 agcaaatgaa gctcttttga caggcgaatc tgatttgatt gtcaaagaaa ctggagctga
    22801 attgctttct ggaagctttt tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg
    22861 tgcagataat tatgctgtcc gcttgactag cgaggcaaag actcacaaag ctattcgttc
    22921 tgaacttgta gattcgattc gcaaagtatc caaattcact agtttcgtga ttattccttt
    22981 ggggatcata ctatttttgg aagcactgtt catccgcgat gcttctatca aaatttctgt
    23041 tattgcatct gcagcagctt tgttaggcat gcttccaaag ggtttggtat tgctaataag
    23101 tattgcatta acgacagggg taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat
    23161 gtattcaatt gaaacgctgg cccatgtcga tacattatgc ttggataaaa cagggaccat
    23221 tactgaagga aaaatgagta tccagcgggt agaagcacta catccgctgt acgagaaagc
    23281 catcccagaa attatgggga cgtatctggc agaaagcagt gataacaaca tcactatgcg
    23341 ggctatgaga gattattttg agctttctaa tcggtatgat gtgacaaaca aagtcgcatt
    23401 ttcttctgat cgcaaatggg gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg
    23461 agcgcctgaa cgtttggtgg aagaagatcg gcttccggac agtctaaaaa cagcccaagc
    23521 gaatggctat cgcgtactta tgttagggat cgctccaaat cagcaattga acgaagaaaa
    23581 accagcattt gttgagccgg ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc
    23641 tcaagaaaca ctagcttatc ttcatgagga aggtgtggat ctgaaagtga tttctggaga
    23701 caatcctttt actgtctcca atatcgctag acgtgcagga ttaccgaatt atgatgcgta
    23761 tgtcgatctt tctcaaatta ctgaagaaat ggaagtgcga gaagctgctc atcgctatac
    23821 ggtttttgga cgagtgagtc ctcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag
    23881 cggcagaacg gttgcaatga caggcgacgg tgtgaatgat gttttggcgt taagagaagc
    23941 agattgtagc attgctatgg cagaaggtga tggagcaaca cgtcagattg cgaatctggt
    24001 attgttggac tctgatttta ctacattgcc agaagtcctg tttgaaggac gccgagtggt
    24061 caacaatgtg acgaaagtct caggcatctt ttttatcaaa acgatttatt catttatttt
    24121 atcgattata tgtgccgtta cagcaattgg atttccgttt atccctatcc aaatcacgct
    24181 gattgattta gcgattgaag ggtatccttc cttcttttta tcttttgaac aagataaaag
    24241 aaaaatcact taccgttatt tgcctacagc acttattaat gctttaccaa atgccttgct
    24301 tgtagtagtg aatatcattg cggtgtatct acttggaaaa acattcgcat ggggaaatgc
    24361 tgaaactact actttgatgt attacttgct gattgggatc agttgcatgg cagttgtaaa
    24421 agcttgttat cccttcaacc cattgcgcat cttcctagca gtaacgacga tcgtagggat
    24481 atatgtagct gcaatgctgt tccatggaat tttggaagta aacttagggt tgggagagag
    24541 cgtaggatac ttctttgtat tcatgctcgt aaatattgtt cttcgagtat tgtactggaa
    24601 ggctgatatt caaaatcagt tactgaaaag agtagcaaat tgatgcttaa tgagatagaa
    24661 atgtgttata cttgaaaaag caatgaaata tgatacaatc aaacatgtat aattgtgtcc
    24721 tttaaaatcg cactgatttc agttagtgcg attttttacc ggttattctc ctgaaatatc
    24781 ttctgttaaa cccaaaagat atcaatagag cagggggtgg aaaaatggct caacggattc
    24841 ctcaagaggt tatcgaaaca gtacgcagtc aaacgaatat cgttgaggtg attggacaat
    24901 atgtccagtt gaaaaagtct ggtaaaaatt acttggggct gtgtcctttt catgaagaac
    24961 gtacaccgtc tttttctgtt gcagaagaca agcagatttt tcattgcttt ggttgtggta
    25021 agggcggaaa cgtgtttact tttcttcaag aactagaagg tctaagtttt ccagaagcag
    25081 tggtaaaagt ggcagacttc gaacagatac cgttagaaga tcaatggcgt cagcgtacta
    25141 tagctgtcca aaatcctgaa tctgctacgg ggaaactgat tgctgctcat gaaaaagcag
    25201 cggaaatcta tcatcacatg ctattgcata caaaagctgg acagccagct ttagaatatt
    25261 tgcaaagtcg tgggttgtct ttagaattaa ttgaagaatt caacataggt tttgcaccta
    25321 atgagcgttc ttttcttcag caagtattca agaatgaatc aatttcaaat gaattgttag
    25381 aacgctctgg attgtttgtc acgcatgatg atggccgtat gtctgatcgt ttttatcaaa
    25441 gagtgatgtt tccacttcgt aattctcaag gccagacgat cggtttttca ggacgctggc
    25501 ttgcacctga aaaagggcag gaaaaagatc agcccaaata cttgaatagt ccagaaacag
    25561 aactgttcaa taaaagacaa gttttattta atcttgataa agcaagaagc gatatccgga
    25621 aaaacggaga aattttgttg ttcgaagggt ttatggatgt gattgctgca tggcaggctg
    25681 gtatcaaaac tggattagct tctatgggga caagtctaac agctcaacag atccaacaaa
    25741 tggaaaagct gacgaaagaa ttggtctttt gctatgacgg agacaacgca ggttttgaag
    25801 caaccaatcg agggattgaa ttgcttcggc aaaacagtcg cttagcactt tctgtcgtag
    25861 tcgttccaga aaagcttgat cctgatgaat atctccgtaa gtatggagaa gcatctttcc
    25921 aagaattagt ggctcatggg cgcgaaacag tctttacttt taaatcacgt tatttaaaac
    25981 aagggaaaaa tttagataat gaaaaagaca agatcgaata cctcgaagaa ttactagttg
    26041 aacttagccg agttgtttca ccaatcgaac aagatatgta tctatctcaa ctgtcaacag
    26101 agtttcagat cggcagagag acgatacaaa aacaactgcg cgatcttcga aggacaaatc
    26161 agcaaaatcg tcaagaacct caggaaagtc aacacactca ggttacgaga caagaaactg
    26221 cacggaaaaa gagacctttg acgcaagtag aaaaggcaga gcagattcta ttatatcgtg
    26281 catttaaaga agtttctgtc cgtaatattt taaaagaacg agaatttcag tttattcatg
    26341 atgcatatgc agaagtttat tttttattcg atgcatatgt ctcacaacat tatgaattca
    26401 accttgcaga gtttttagac tttttgcaag atgaaaattt aaggcggttg gtggtggaaa
    26461 tcgaatattt accggtggca gaagaaagtt cgccacgaga aatccaagac ttgctagatg
    26521 tgatcagcaa atctggctta gctgatgaaa taacattaaa aaaacaaatg cagcaagaag
    26581 ccagacgaac tggaaacaaa cagcttgagc ttgaattgac gattgaaatc atcaatttaa
    26641 cgaaacagtt aaaacaagcc aaatagctac tcaagggggg ccttctttta tggcaaatgg
    26701 aacagataaa aaaacgtatg aagcagcagt agcagctttc atcaaagaaa acaaacccaa
    26761 acgacaagtt gtatatgatg atttatccaa tcagcttgct acaccattca ctttgaatgc
    26821 agatgaaatg gacaaattga tccaaaagat tgaagacgca ggtatcagtg tggtggatga
    26881 aaatggtgat ccatcaattc acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa
    26941 aacagaagat ctatctgcgc caaccggtgt aaaaatcaat gacccggttc gtatgtatct
    27001 gaaagaaatt ggccgtgtct cccttttaac tgcggaagaa gaagtagcgt tagcattgaa
    27061 aatcgaagaa ggcgaccaag aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt
    27121 agtaagtatc gctaaacgtt atgtaggacg aggcatgcag ttccttgact tgatccaaga
    27181 aggaaacatg ggactgatga aggcagttga aaaatttgac taccgcaaag gatttaagtt
    27241 ttcaacctat gctacatggt ggattcgtca agccatcact cgagcaatcg ctgaccaagc
    27301 acgtacgatc cgtattccag ttcatatggt tgaaacaatt aataaattga tacgtatcca
    27361 acgtcaattg ctgcaagacc ttggaagaga acctacgcca gaagaaatcg gtgcggaaat
    27421 ggatcttcca acagaaaaag tccgagaaat cttgaaaatc gcgcaagaac ctgtttcact
    27481 tgaaacgcca atcggtgaag aagatgactc tcacttaggt gatttcatag aagatcagga
    27541 agcaacaagt ccagcagaac acgcagctta tgagttacta aaagaacaat tggaagatgt
    27601 actagataca ttgactgacc gggaggaaaa tgtccttcgc ttacgttttg gtttagatga
    27661 tggccggacg agaacattgg aagaagtagg gaaagtattt ggtgttactc gggaacgtat
    27721 tcgtcagatc gaggctaaag cattaagaaa attgcgccat ccttctcgtt caaaacaatt
    27781 aaaagatttc ttagaataag catgaactat tgaccagaag agaaatcagt aaaactgttt
    27841 ggtttatttt aaaagaaaaa aagcttgaga ctacgcagtc tcaagctttt ttttctatag
    27901 gaaggaaatt cattcattat ttttaagtaa atcctaaaaa taagctattg gcgtgacgta
    27961 tagaatatga tagattaaat ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat
    28021 gaaaaaatgt ccaaattgcg gatcgaaaaa aatggagcag aatactatct gtccagactg
    28081 cggtcatcaa ataactgcag aagatcagca aataaaagaa tcaattgaag aaacaaatac
    28141 agatgcagct atgcaggata aatcaactga agatgcagtc tttcctgata cagaattaaa
    28201 cgatccaatc gaatggtcgg aattgaaaga tttacctcta gaatctgtaa tggaattatt
    28261 cgaaaaagca gaaccagaag acgaagaacc acctgctcac gatcaagtaa ctaatataga
    28321 gaaaaatgaa cagaagaaag caactgttca tgagacttcg gaagaatcag gaaagaatca
    28381 agaagaaacg cttgaatcaa aacaaaaaag aagagtagct gaactgaaag aaatcgtcga
    28441 taatgaggaa gaaaattcga tactatccgc ttatatcaaa gcacatcgtg aagatacaaa
    28501 agaagagcac gcgaaagagc tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa
    28561 agtagagcca gaacatgaag ctgaaaaaga cactgaaaaa gacattagtg gagtaaacgc
    28621 tgcaggtaaa gaatcattaa aaacgaatga tctaaaaacg aatgatctaa aaacggatat
    28681 cccggaaaaa actgaaacat tgtctgaaaa acaatcagaa gacgagaaat cgaatgggtt
    28741 gccggaagca gataaaaaag aaatgctcga aaaagcagac aagaaggcaa cagagtcgga
    28801 tggaataaat aatgaagccg atgctgaaac tcaatctgca agtgaagata gcactgctct
    28861 tgaagctaaa atagaaaaag aagagaacca gcctgagctg attgaagcag aaacagaacc
    28921 aataacaatc gtgccaaata ctgaaaaaac gcctcaagaa tcaaacccag aaaaaaaaga
    28981 tttagataag acacaaaaga aaaaatcaaa aaaagtgccg tatctcgctt tagcagcagt
    29041 tcttctacta ggtgggggag gctgggctta ttatgaccat catcaaaaag tagaagccga
    29101 agtagcagca gaagcaaaaa gacagcaaca aatggaagaa cttcatgaga aacttgctgc
    29161 cttttataca gatgatcacc atcaatttat tcgtgcagat atgattcatc aagatttatc
    29221 aaaactaaaa aatgatttat ccagtcttaa gaatgaaaaa ggatattcag agttagaaac
    29281 aacttaccaa gatatccaat ctaagatccg agatatccaa aaagtgaatg agtggttcat
    29341 agcacctgcc atcgtcgatg atcatctagc agatgacatc aagttgaaag cagataaaga
    29401 gatccaagca atcgaaacgg ataacacaga ttttggaaaa ttgattcaac aagcacaaac
    29461 agaagctaaa aatcaatatg ctgaattgca aacagctaaa gaaaaaacag ctatagtctt
    29521 taaagacgga aaagtagtgg attcagcaac acgcgaccaa tacaagacgg caaaagaagc
    29581 agtagaaaaa gtgaaaaata cagatctatc tgctcagttg aatgaacaat tgcaacaagt
    29641 agataagatg ctcactgata aagagaaaaa agaagaagag gaaaagaaaa aagcagaaga
    29701 agcgaaaaaa gcagaaaaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
    29761 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
    29821 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
    29881 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
    29941 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggat acagattaga
    30001 aaaagccaga atcgaaaatg gggaaggcta ttacaatcta tatgccactt caacaaaatc
    30061 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
    30121 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
    30181 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
    30241 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
    30301 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
    30361 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
    30421 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
    30481 agcagtggaa ggattcggct accaaaatca aaaacatgaa aatcggatca ctacacaaat
    30541 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
    30601 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
    30661 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
    30721 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
    30781 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
    30841 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
    30901 acgatataat gaacctcgtc taggcgagca aataaaaggt cgagtgattg gtgtccgacc
    30961 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
    31021 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
    31081 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
    31141 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
    31201 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
    31261 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
    31321 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
    31381 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
    31441 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
    31501 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
    31561 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
    31621 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
    31681 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
    31741 catggaatct gtcaggaatg ctatgaaaaa gaaaaaagaa accagtccgt ctgagtgtct
    31801 agacggcttt ttctttgctc agattatgga aaaagactat cagaatgaag cggtatctga
    31861 tatgatagag aggcatgagg gggattatta tgaaagagca aatcaacgat tatctccact
    31921 acttgaatat tgaacgggga ttgtccgcca atacacgaca aagctatgaa cgtgatttgg
    31981 aacagtactt gcattttcta caggagcaga aaattgcttc atggcaggat gtcgatcgct
    32041 ttttagtcat ccgttttctg gagaagttgc atgaagaaaa aaaagcgaca gcaactgtta
    32101 ctcggatgat ctcaagtctc agaagattcc atcagttttt acggcaagag cgtatcactg
    32161 atcatgatcc gatgcaacat atcgatacgc cgaagaaagc acagaaatta ccaagtacat
    32221 tgactctgac agaagtagaa aagctgattg aagcacctga tacgacaaaa acgttaggga
    32281 taagagatcg tgcaatcttg gaagtcatgt atgctacagg gatgcgcgtc agtgaattga
    32341 ttggattgaa attgggagat cttcatcttt ctcttggact ggtacagaca gttggaaaag
    32401 gagataaaga gcggatcatt cctttaggtg attatgcgat ccaatggctg gaacgctact
    32461 tagacgaagc acgtccatta cttgtaaagg acgcatctga aatgcatgta tttgtcaata
    32521 atcatgggaa aggtctttct cggcaaggaa tctggaaaaa tctgaagcag ctagtacgag
    32581 aggctgggat ttacaaagaa gtaacaccac atacgctgcg gcatagcttt gccacacatc
    32641 tgctagaaaa tggagctgat ttgcggacag tacaggaatt gttaggtcac gcggatatct
    32701 ctacgactca gatttataca catatcacga agaaaagaat gacggatgtt tataagcagc
    32761 attttccaag ggcttagtca taggaaggtg agaagatgtt aacacattac cggaaagaaa
    32821 accgtaaaat tgctatggga ctattgagtt tccataagaa actgacaggt aaaacttctt
    32881 tgttgaagga gattgatacg tacgaatctg aagacgatta tgaacttttt ttctttacgc
    32941 cagaaggttc aaccaacatt caagggatta ttggtgttta ctgggcaacg aacgatcagc
    33001 tgatcatcca tgacatttct ttgaatccgt cttatcgcgg tgagaatatg ggctttcaaa
    33061 tgttagatga attacaggaa aagtacccgg atgtctccat ccgtgcaact gaaatcacag
    33121 agccttacat tggaaaatgg ctagcaaaga aaaagaagaa agagtgaatg agttgacgga
    33181 gatcaatatt aaattagatg tattcgaagg tcctctcgat ctgctgcttc atctgattca
    33241 gcaaatggaa atcgatatat atgatatacc gatcgctacg atcacggagc aatatatgaa
    33301 ttatatccat acaatgaaaa cactagagct agctgttgca ggagaatact tagtgatggc
    33361 tgctacattg atggcaatca aaagcaagac attgcttcct gtccaagaat tcgtcacaga
    33421 tgatgaagaa ttatgggaag aagatcctcg agaagaattg gtcactcagc ttttagaata
    33481 ccgtaaatat aaatatgcag ctgaaagact gacagaaaaa gcagaggaac gaagtcttta
    33541 ttacacaaaa gaacctatga acatcgatgg gttagcggat gctgaaaagc cgttgaagcg
    33601 aggacaagtc aaaaaaatcg atttgatccg tgccatgcag aaaatcttag aacgtaagaa
    33661 attttctcaa aaagtggaaa aaacgatcac gcccgatgac acaacgatcg aagaacgaat
    33721 ggtatttatc gaagagcgta taaaaagaaa tgcgcatggc tgtactttcg attctttttt
    33781 tgagtcacct tctaaaagtg aagttgtcac tacttttatg gctttgctag aattgatgaa
    33841 aaaagggcag atcattgcgc aacaaaaaga aaactatgaa tcaattatgc tttatccagc
    33901 agtaggagat accgaacatg acgaaattaa gtgaacttga agcccttctt tttattgttg
    33961 gcgaggaagg gatcagtaat gaagaattaa gcggactgtt aaatctcagc gaagcagaag
    34021 tcacaagttt agtgaaagaa ttaaaagaat cttacactca aaactcgcac tcagctttgc
    34081 atatttttga aacggaaagc cattatgtat taacgactaa aaaagagctg gctcccttac
    34141 ttaagcgata tgcgcaaggg acatcaaaca cattatctca agccgctgtc gaaaccttag
    34201 caatcatcgc ttataaacag ccaatcacaa gagctgaaat cgagcagatt cgaggagttc
    34261 aggtttcagg agcggtacag cgtctagctg cccataaact gattgaagaa aaaggtagag
    34321 tagaagggcc aggtcggccg atattatatg gaacaactgg ctacttctta gattattttg
    34381 gtttgaaatc attagaagaa ctgcctgata cacaagaaat ggaaatttca tttgaagaag
    34441 acgtgtcacc agatctattc tttgatggaa tgaataaagg agaataattg taatggaaag
    34501 attgcagaaa gtaattgccc acgctggcat cacttctcgt cgaaaagcag aagaattcat
    34561 ccttgcagga cgtgtaaaag taaacggaca agttgtaagg gaattaggga taaaagttgg
    34621 gaaacatgac actgtggaag tggatgaagt cccaatttat caagaagaat acggttatta
    34681 tttattgtac aaacctaaag gagttatttc tgctgttgca gatgacaaag gaagaaaagt
    34741 tgttacggat cttttgccga tggtaaaaga acgtatctat cctgtaggtc gactggatta
    34801 cgatacttcg gggatcttgc tactgacaaa tgatggagat tttgctcagc gcctgacaca
    34861 tccaaaacat gaagtggata aagtttacgt tgcaaaggta aaagggatcg ctacaaaatc
    34921 gatgcttgca cctttgacaa aaggaataaa gatcgaagga cgaaaaacat ctccagctaa
    34981 attccaaatt ttatcagtgg atcctaagac aaaccacagt atcgtggaat tgacgatcca
    35041 cgaaggacgt aatcatcaag tgaaaaatat gcttcaagca gttggtcttc ctgtgcaaaa
    35101 actaaaacgt gaaaaatacg gagatctgac tttacaaggc ctacgtcctg gagattaccg
    35161 tgaactgaac aaaaaagaag tcagcagttt gctgaaccac tcaaaatgaa cacttacttt
    35221 ttataagtgt tagatgttgc gttttttgaa tagatatgta taatgaatat gtaaacaaaa
    35281 attatataag gttcgtcttc aggggcaggg tgaaattccc gaccggtggt tatagtccac
    35341 gacctatttt gcaaaaaata gctgaatcgg tgaaattccg ataccgacag tatagtctgg
    35401 ataaagaaga tagagcttat ttgacgacgc ttgatcaaat aactctaact ctattttccc
    35461 tggaaaatag agttttttca tttggagaga aacgtcaaaa agttcgctga agaggagtcc
    35521 taacaggagg atttcaaatg aagaacaccc gtgtacagaa aatggttgca gtagcgatgt
    35581 ttgctgcgat cgcgttagtt ttacagtatt ttgcttttcc gatcatgccg gcttttagtt
    35641 ttttgaagat cgattttagt gatatcccgg tattgatcag catgttctta ttcggtccag
    35701 cggccggagt tgcaacagcg ttcttgcgtt cagtattaca tctgctgact acaggttttt
    35761 ctccggataa tttggtagga gacactgcta gtttcttatc aacaacgatc tttacattcc
    35821 cgatttatta tttctttagt gtaaagaaac agcataaagg aagaaacaaa tttttaggtg
    35881 tcattacagg aaccatcgct ctaactattt ttatgagtat cgcaaattat ttcgttatta
    35941 cgcctcttta cttaatgttt ttaggactga acgcaacaca gatgctaggc atgtctttag
    36001 caaactatgt actgattggg atcgttcctt tcaacttgat caaaggattc atcgtgagcg
    36061 ctgtcttttt agtgctgcat gcgcaacttc ttccatggtt aagtcgaaaa caacatcagt
    36121 tcgaacaacg tcatacagca gtcaaataat tatcaaaaag atcaagaaat ttcttggtct
    36181 ttttttttgg aaaaaaatac aagacatgca ataatgtaaa cgttttattt gtttgttttg
    36241 agtatttttt tgatttatgc ataaaaatca atcaattctt tgcaaatgtg aaattcttct
    36301 ccttttaaaa gacgaaaatg tttgtttata aaaaaaagga aacgctacaa tgaaaaagaa
    36361 gaaagcttta aaaacctgaa aaaagaggag ggaataggga aaaacgaact ttttgaacaa
    36421 aagttttaca aaatgcgtgt tattattttt gttctgatac tctttttgat aaaataacat
    36481 tagagccaaa attgttgtct ctaacggaaa agaaaggaag aagaagatgg aacaaacaaa
    36541 aaagaaaaaa tttcaaatgc cttcagcgta tacggtctta tttattatta ttgcagtaat
    36601 cgctgtactt acttggttta taccagctgg gcactatagt gtagacgatg caggcaacat
    36661 cgtcgctgga tcatatgaac gagtacaaca aaatcctcaa gggctgtggg atattttcat
    36721 ggcgccaatc aatgggatgt taggtgtacc agcgggggag ctaactaaag aaacacctgc
    36781 tgcaatccca atctctttct tcattttggt tgttggtgga tttttaggta ttatcaacaa
    36841 aacaggggca ctagatgccg ggattgcttc agttgtgaag aaattcaaag gaaaagaaaa
    36901 aatgcttatt ccagtattga tgctgttatt tgcattaggc ggctctactt acggaatggc
    36961 agaagaaacg attccattct atgctttact gatcccagtt atgatggctg taggctttga
    37021 tacagtagta gcagtagcaa tcgtattagt agggtctcaa gtaggctgtc tagcttcaac
    37081 ggtaaatcct tttgcaacag gcgtagcttc acaagctgtt gggatttcga tgggtgaagg
    37141 tattggtctt cgattcttga tgttcgttat tttgttagct atttctattg tatatgtgta
    37201 tcgttatgca tcaaagatcg aaaaagatcc aacaaaatca ttagtataca accaacgtaa
    37261 agaagatatg aagcatttcg atattgaagc aatcggtcgt caagaagtaa tcacgaaaaa
    37321 acaaaaacat gttaatgtat tgttctttat tacttttggt atcatgatca tcagtttgat
    37381 tccttggaca accttgaatg cgaatttcac gatttttgaa tcattgacaa actggttaac
    37441 aaaccttccg gtactaggta ctgttctagg taaaagcatg cttccattag gtgactggta
    37501 cttcccagaa attactatgt tattcttagt aatgtccatc gtagtagcaa tcacttatgg
    37561 catgaaagag tctgaatttg tctctgaatt tttagctggt gcttctgatt tgatcggtgt
    37621 agcaatcgta gtagctgttg ctcgtggtat ccaagtggtt atgaacaatg gattaatcac
    37681 agacaccatc ttacattggg gcgaacaagg attaagttca ttatcttcaa gtgtattcat
    37741 cattattaca tttattttct atatccctat gtcattcttg attccttcaa cttccggctt
    37801 agctgctgca acaatgggaa tcatgggacc tatgggtgaa tttgccggcg tcggtaagga
    37861 tttagtaatc actgcttatc aatctgcatc tggtttagta aacttaatta caccaacatc
    37921 tgctatcgta atgggtgctg tagcaattgc tcgtttcgat gtttcagtgt ggtggaaatt
    37981 taccggtaaa ttaattgcga tattatttgt agccatctgt gtgatcctag gtgtagcagc
    38041 aatgttctaa acctagacaa atacaggtac aaggtctatc ataagaatga agaaaccgat
    38101 aaacggctat cgataagcac gtggacaagt caatagctcc attcagtgag tgttttttct
    38161 ttcatcattt gttaagaaca ttctgcttgc tgatggagca ggcttaaaac gtcattattt
    38221 gagaagaaga ggttgtgtcg gaggaatttg acatgaccct ttttaacata ttaggaggat
    38281 aatatcatga aaaaatttgt gacagaacaa catcatgaac aagcattaga atcattgagc
    38341 gaattgatcc gaattccttc cgtactagat gaagcagatt caggacaagg acatcccttc
    38401 ggtaaaaaag tgattgaagc tttggataaa gtattggaaa tcagtgaaaa gctaggattt
    38461 agaacattta aagatccaga aggctattac ggttattcag agattggctc tggagatgaa
    38521 ttattcggta tcttgtgtca tatggacgtt gttccagcag gggacgaaaa taattgggaa
    38581 acaaaaccat tcgatccaac tataaaagac ggctggttag ttggtcgcgg atcccaagat
    38641 gacaaaggtc cttctattgc ggcaatgtat gccgtcaaag ctttgattga tgctggcgtg
    38701 gaattcaaga cacgtattcg ttttatcttt ggtacagatg aagaaaattt atggcgttgt
    38761 ttagaaaaat ataacgaaaa agaagaagga attacgcaag gatttgcgcc agatgctgaa
    38821 tttcctttaa tttatgcaga aaaaggctta ttgcaagctt acttgacagg accaggaacg
    38881 aacgagtttt ctgtaaaagc tggaggagcg ttgaatgttg ttcctgatgc agctccttac
    38941 tctggagaaa agctttctga agtaaaagaa gcgttgaaaa aacacgaatt cgatttcgaa
    39001 gaacaaggtg aaggcatcgt cgttcttggt aaaagtatcc acgcgaaaga tgcagcacaa
    39061 ggagtgaatg ctatttctcg tctggcaatt gctctttcag aagtattcga ttttggccca
    39121 atcaatttct taggaaaatt agttcaagaa aatgcaactg gtgaagctgt cgtgggcaaa
    39181 acagaagatg aacaatccgg cgagctaacg atgaactttg caagtttgga aatcacacca
    39241 gaacaaacaa aaatcggtgt agatatgaga atccctgtta cattcaagaa agatgattta
    39301 gtagctaaat taactaaaac agcagaaaaa tatgggttga cttatgaaga atttgacttc
    39361 cttgattcat tatacgtgcc actagacagt gagttagtga aaaatctatt ggggacatat
    39421 cgcgatatta ctggagacat gactgaaccg tttgtatcag gcggcgctac ttttgctcgt
    39481 acgatgaacc agtgtgtggc attcggtgcg atgttccctg atactcctga ctttatgcac
    39541 caggcgaatg aacgatggga attaagtagt atgtataaag caatggaaat ttatgcagaa
    39601 gctgtgtatc gtttatgtgc taaataaaaa taaacataca aaaagggcgg atattcaaat
    39661 ggatattccg tcttttttta tttgatgctg gaaagtttgc atttctctct tatttttttt
    39721 aaggtataat catacttgat taagaaaaag gggtcactca gatagaaaag gtgggagaaa
    39781 gtgaaaatat ttagacgaat actaattggc ttagtcatca ttttaggatt actcggtgga
    39841 gccggttatt actatataca gcaaaatacg tacgaaccat cgtcagaagc gcaagtgttg
    39901 gtaaaagaag gaaaaaacaa agaagattac ttattttttg aaggagagaa aaagaatcat
    39961 ggaaattctc cattacttat tttttatcct ggtgctctcg tagagccaga aagttatagt
    40021 atttgggcgc aagatttagc aaaaaacggc ttttcagtcg ctattgtcaa aatgccactt
    40081 gatctagcag tgatgggcgg aaacaaagcg gataaggtca aagaagattt tccaaattcc
    40141 gattatgtga tcggtggcca ttcattaggt ggcgtaatgg caagtcgtta tgtcaataag
    40201 aatcattcag acaaacattt aaaaggggta ttcttcatgg caagttatcc cgacaaaaaa
    40261 ggaacattga cagaatcagc tgttcctgtt ttatcgatca cggcgacaaa agataaagta
    40321 ttgaatcagg aagcttatca agaagctaag cagtatttac cggaaagaac agagtatgta
    40381 tcaatcaatg gcggaaatca tggaggtttt ggcagctatg gtgagcaaaa aggagatgga
    40441 gaggcagaaa tttctaacgc agaccagcaa actgtgatcg tcaatacatt ggaaaactgg
    40501 ctgaaaaagt tgaaataata taaatgggtc ctatagggtc cgtgaaaaaa ctagtcaatt
    40561 aactatagat agcaaatgaa tctttcaaaa taagtcggga tttcacggaa caagagaaac
    40621 agatcccggc ttattttcaa gtttagagaa cttagtgtac gtttttaaag tttcgtttat
    40681 atttatattt tattttctaa taaaatcgct cgaactggac atttttgata cgcttttttt
    40741 acatatgttt cttcttccgg catcacgaac tgttgatgag cctctggttc ttgggcaaat
    40801 aaaacgattc cttcttcatc ataatcgaaa atatctggcg cttcgatttg acaaagaccg
    40861 catgcaatac agcgttcagg tacaagttta catagtagtg acatttctaa ccatcctaac
    40921 ttaaggagaa taatatgatt gtttttattt tatcgctatt ttcatcagga cacaagctgc
    40981 ggattagttc tctttaccaa ctccttgtgg gaaaacgaac gacttcagta cttatttatg
    41041 gatttaccca cgaacttttg tttatccata acagttttcc agaattgaag caggataagt
    41101 tttatcaaat tttgcaaaaa attgcgcagc aaggttggat cgaaataaat gaaaatgaag
    41161 caaagctaac gcctgcaggg gctgatatgt tgtccgaaca tcaaatagaa catactggtt
    41221 tgcgttttga ccgctatggc agaacagggg aaactagttg gcggctcatt aaatttgctg
    41281 ttcaagtaat ttctaatctg gcaacgggta ttcaagacta tcttccagca gagacaagtc
    41341 ctttttatac tttccaacta aaaaaatggc ttagtgaaag ccaattgcca cgtgaaattt
    41401 tgattgatac tgcgtatgag agtttggtgc agctgttttc ggagatacca gaagaagcag
    41461 cagatttttt agctaaccaa ttatctggaa atgagcgaac agggctgctt ccttatcagc
    41521 tggcaaaaaa caatgatgag tcagcggttt atctgcaaca aagcagatgt atccaccttt
    41581 tgttagcaca aatcgaaaaa cggccagatt cgctttggca cgctttgatc gattcattat
    41641 tacagcaaaa tttcaatcaa agcatgatga tcaccaagca gatgtttatg aatggtcaga
    41701 caattgacca gattatggca atccgtcatt taaaaagagg aacagtaaca gatcatctta
    41761 ttgagtgggc cttgtttttt gatgattttc catacgagcg gattctctct gcagaaacaa
    41821 tcgaacgttt ggaaccaaat aaggattccg tgcgagagtg gcgctttagc gaatggaatg
    41881 tagatggaca attagattat ggagaatttc gtttgtatca aatctatcta ttgagaaagg
    41941 aagcgataca gaatgttaat aaatgaatta aaaaaatatt ttggatttga ttcatttcgt
    42001 cccggccaag aagaaatcat tcaggcgttg cttgatggag aagatactct cgctattttg
    42061 cctacaggaa cgggaaaaag cctttgctat caattgacgg ggtttttaat ggaaggtctg
    42121 gtcatcatcg tgtcaccgct gctttctcta atggaagatc aagtgacgca attacaaaaa
    42181 cgaggggaga aacgagtagc tgctttcaat agtttacttt cgcaatctga aagaagatac
    42241 gtcctccgtc atctatcgca atataaattt ttgtttttga gtcccgaaat gttgactaat
    42301 ccttctcttt tagagcatct gaaaaaacaa aatattgcgt tatatgtagt agatgaggct
    42361 cattgcgtgt ctcaatgggg agttgatttc cgtccagaat atcaacagtt aggaaaaatc
    42421 cgaaagcttt taggaaaccc ggtcacactg gcactaacgg cgacagcaac agatttagta
    42481 gcaagagata tacgtcatgt actatttgat cgaccaccaa aagagatcag acaatcagtc
    42541 aatcggcaaa atatctcgct ttttgttcgg aaaacgcagc aaaaagaaca agaacttgaa
    42601 cacttcatgg aaagagcaca aggagcggca atcatttatt gtgcaacaaa aaaagaagtc
    42661 gaacgattgt atcatttatt cagggaacgt tttacagttg gatactatca cggtggtctt
    42721 gatgcagctc aaagaagaca acttcaacag cagtttgtaa aaaatcagct gcagtttctc
    42781 attgctacga atgcatttgg tatggggatc gataaatctg atatccgtta tgtgatccat
    42841 tatgatttgc cagacagtct agaaaactat gtacaagaaa tcggtcgagc aggcagagat
    42901 caggaagaaa gtgcagcaat tcttttttat caaagtgggg atgaacgtat ccattatttc
    42961 tttaatcaac tttctagaga acagcgacaa agttttgagc tttatttgga atatgccaca
    43021 gagcaagctc cctttgatga gctgcaaaaa aaatggatgg agctgatcca gcagtctgaa
    43081 aaaccagaaa attgggtgga acggctcaaa catcaggaaa aagaaaaaga attccgcctg
    43141 cagcagatgc ttcgctatat taatgaagaa aactgccgca gaaaattcat cttagcttat
    43201 tttggcgaaa aactctccga aaagccgcag aattgttgtg atattgatgg agcaaaagca
    43261 gaacttctgc caaagaggaa gcttttttct aagactgaac aacttcattg ggaaacgatt
    43321 ttactaaatt tatttaaaaa aaacatggaa cattgatttt ttaggacaag acactttttc
    43381 ctatggtata atacacacga tagagtttag gaggtaaaac agtgagcaga aaagatagac
    43441 atcaatctac agaaaataac gaaacacaag aaccatggga acaaccaatc tacgatacag
    43501 atgatgaagt ttcatcaaga agttctcaga gacaacaaaa gaaaggaaat acattatttc
    43561 tttcgctatt tttgatttta ctggtcttat gtatcgctat tccagcaggt gcttatttct
    43621 ggatcagaaa tggatcaagc aacaatgcaa cagcagcaac ttcttctgaa acttcgacat
    43681 cagtagtaga aagttctaca agtacaacaa aagaggagtc aactgagact tcaacagtag
    43741 aatctagtac aatcgcggaa gaatctactg tgtctcaaga tacaggtgta acgtcagaaa
    43801 gcagtattgc agcagagaca acaccgactt cctcaagtga ggcaacttat caagcagggg
    43861 attcaagcag caatgcagca gaaacaacac cttcttcatc agctgcagcc ggttcaacaa
    43921 cgactgttca agcaggcgaa ggtccaaaac aagtggctga gcgtgcaggg atcaccactg
    43981 atcaattgtt tgaattgaat ggcttagatc caaacaactt tatgctttac cctggtcaag
    44041 aattaagaat taaataataa tagccgcgct ttgcggcttt ttttttgaga aaaaggagtt
    44101 atatcatgac acaaatcagt attgcaatcg acgggcctgc ttcatctggg aaaagtaccg
    44161 ttgccaagat attagcaaaa gaattgaatt atatttatac agataccggt gccatgtatc
    44221 gcgcagtaac ttatttagct attacgaaca atgtatcctt ttcagatgaa gaagggctag
    44281 tttcgctgat caaaaagtat cctatctctt tttcgcaaca agaagatgga cagcatgtat
    44341 tcatagcatc aaaagatgtg actttagaaa tccgtcaacc agatgtcaca aaagctgttt
    44401 ctgaagtctc tgcacatgga aaagtcagag aagaattagt tgctatccaa cgaaaaatag
    44461 gggaacgcgg tggcgttgtc atggacggaa gagacatcgg gacagctgta ttaccgaatg
    44521 cagaagtgaa aatcttttta gtagcaagcg taagtgagcg tgcagaaaga cgtttcaaag
    44581 agaaccaaga aaaaggaatt ccgactgatc ttgaaacact gacgaaagag atacaggaaa
    44641 gagaccatta tgattcaaca agggacgtct ctccgctgaa gcaagcagag gatgctgttc
    44701 gtatcgatac gactggcaaa tcgattccag aagtcgttgc agcaataaaa gctgttgttt
    44761 tgaaaaaagg ttatcttctt ttttaaatta agaaaaaaaa tgaaaaacaa ttgttttttc
    44821 aaggaaaacg aacgaaatcg ctttattttt tttgaataat caattaaact aaaaacagta
    44881 ccgtacttac ggcagaatgt tggttaggag gacaagtgtt atgacagaat ttgatcaaaa
    44941 tcaagagaac aaccagtcaa tggaagacgc aatgaaaagc gtacaagaag taaaagttgg
    45001 cgatgttgtt aagggcgaag ttttagtcat tgaagacaaa caagtgattg tcggaatcga
    45061 aggggctgga gttgaaggag tcgttccggc aaaagaatta tcaactttgc cggtagaaga
    45121 tatcagcgaa gtagtaaaag tcggtgatgt attagactta gtcgttatca gtacgatcgg
    45181 caaagataaa gaaaacggca gctacctact ttcaaaacgc cgtttagatg ctaaaaaagt
    45241 ttgggaagat atcgaaagag atttccaaga gggcaaggtg atcgaagcac ctgttacaaa
    45301 tgttgtcaaa ggcggtttag tcgttgacgt cggtgtacgt ggttttgtcc ctgcatcaat
    45361 ggtggaagat cattttgttg cagacttttc agactataaa ggcaaaacat tgagctttaa
    45421 aatcatcgaa atcgaaccat cagaaaatcg tttgatctta tcgcataaag cggtagtgga
    45481 aaacgaaaga gaagaaaaga aaaaagaaat tctttcaact atccatgagg gagatgttct
    45541 cagtggacgt gttgcacgtc ttactgattt tggcgcattt gtcgacctag gcggaattga
    45601 cggtctagtc catgtctcag agatttcaca tagtcatgta gcaaaaccaa gtgatgtatt
    45661 ggctgtcaat gatgatgtaa atgtcaaagt gttgtctatc aatcctgaac aagaacgtgt
    45721 ttccttgtcc atcaaagaca cacagcctgg accttggtca gaaatcgata caaaagctcc
    45781 agttggcaca gtattagacg gaacagttaa acgtttgaca agcttcggtg cctttgttga
    45841 agtgttccct ggagtagaag ggctagtcca tatctctcaa atttcacata aacatattgc
    45901 gacaccacat gaagtgcttc atgaaggtga cgaagtgaaa gtgaaagtat tagagatcca
    45961 tccagaagaa catcgtattg cattgagtat caaagcttta gaagaaaaac cagcttcaga
    46021 agaagaacca aaaaatgttg agtcttatga attaccagaa gaaaatactg gatttactat
    46081 gggagacatt ttaggtgatg cgttgaaaga agaagtagaa gaaaaagcaa ccgatgattc
    46141 tgaaaaataa ttgattttgt atctgtgact gagatgaaag cagctgcttt tgtctcagtt
    46201 tttcttttga aaagcgatta gtatttcaat aaggaataaa aatacttcgt ttaattgaca
    46261 atctatgctt gaaagttggc aatgcattcg ttaaactaag tgagattgga aacttgtgag
    46321 gaggaaataa tatggcaaat ccaacaattg caatcgttgg ccgcccgaac gtcggtaagt
    46381 ctacgatttt taaccgcatc gctggtgagc gtatctcgat tgtcgaagat actcctggag
    46441 taactcgtga tcgtatttat gcaaaaggtg aatggttagg tcgagaattc agcgtgatcg
    46501 acacaggcgg aattgatctt ggcgatgaac cctttatgga tcagatcaag catcaagcag
    46561 aaatcgcgat tgaagaagca gatgtaatca tttgtgtagt cagcggacgc gaaggcgtga
    46621 cagacgcaga tgagatggtt gcgaaaattt tatacagaag taataaacca gtgattttag
    46681 cagtcaataa agtagataat ccagaaatga gaaacgatat ctatgaattt tattccttag
    46741 ggctaggcga tccttatcct gtttcaggaa gccatggact agggattggt gatattttag
    46801 atgaagcagt gaagtatttc tctgaagaga ctgaagaaga ggacgacgac acgattaagt
    46861 ttagtttgat tggtcgccca aatgttggta aatcctcttt gatcaatgcg attttaggag
    46921 aagaacgtgt aattgtttcg gatattgaag gaacgacccg agacgcaatc gacacccact
    46981 tcgtttctga aagtggtcag aaattcttaa tgatcgatac agctggtatg cgtaaacgcg
    47041 gaaaagtata cgaaaatact gaaaaataca gcgtgatgcg tgcaatgcgt gccattgacc
    47101 gttctgatat cgttttgatg gtactgaatg ctgaagaagg gattcgggaa caagacaagc
    47161 gagtagcggg ttacgcccat gaagctggtc gtggaattat tatcgtcgtg aataaatggg
    47221 atcttgtaaa gaaagaaacc aatacaatgc gtgatttcga acaagagatt cgtgatgaat
    47281 tccggtatct tgattacgct ccaatcgttt ttgtttctgc agtgactaaa caacggttgg
    47341 aacgtctgcc tgaaatgatc gaacaagtaa gtatgaacca gaatcttcgt atctcttcgg
    47401 ctgtgttgaa tgatatcatt atggatgctg tagcaatcaa tccaacacca acagataaag
    47461 gaaaacgcct gaagattttt tatgctacac aagtcgctgt caaaccacct acatttgtcg
    47521 tttttgtcaa tgaagaggaa ttgatgcatt tttcttatga acgtttctta gaaaatcaaa
    47581 tcagaaaagc atttactttt gaagggacgt ccatccgaat tatccctcgt aggagaaaat
    47641 aggcatttta ccatatcgac gttttttttt caaaagaaaa agacagtttc gtttgttacg
    47701 tttctatgac taaaaaaagc atgtgaaaat tggtaaaaac cttgctatta agcggttcat
    47761 atgatatcgt gataacagaa tattgaagtt aatcaatatt tctttttaaa cttctggacc
    47821 actcagaaag ttttaactaa atgatatttc tatcatatct cttgtggagg aggtgaatct
    47881 atccatggca aacaaagcag aattaatcga aaaagttgct tcagctactg atttaactaa
    47941 aaaagatgca actgcagcag tagatgctgt attttcaact attcaagatg ctttagctaa
    48001 cggcgaaaaa gttcaattaa tcggttttgg taactttgaa gttcgcgaac gcgcagctcg
    48061 caaaggtcgc aacccacaaa ctggtgaaga aatcgaaatt ccagctagca aagtacctgc
    48121 attcaaacca ggtaaagcat taaaagacgc tgttaaataa gacagttgaa aaagcgcttg
    48181 tggctctagg gctacaagtg cttttctttt tatttcctag cagaaaagga ataaataaga
    48241 gacagagatt ccgaatccgt atgtcataca tagaaaacac atgtatttta tttaattttt
    48301 atttatttcc aagttatgag gtgagctttg ctcttatctt ttctagaatt tgcttactaa
    48361 tggtaaaata aaaataagaa gaggagggat gcgtatggaa cttgattatc aagtagaaga
    48421 tgcgttgacg aaagtgtttt acggagaaac gctagtcagc cagctaagag aactttccat
    48481 tgatcagcca atcttatttt taacgaatca gcgctattat gatttgtttg cagataagat
    48541 caacccgtta tttgcaaatc aagcaaacaa tgactggtat atttgtgcca acactcaatg
    48601 caatcattta acagaattaa agaacctgct tgatttcgtc aaacgctatc ccgaaaatca
    48661 atccatgttt atcatcggat ttggaaatga agggatcatg gatctggcag gtttttttca
    48721 aaaacataca acccttgatt catcactatg gatgatccct gtatcgatcc gttctctaag
    48781 ccgagcatta acaccgcagc ggacgatttt gcagctacca aatcgtgtag tactgcaaac
    48841 catcaatctg ccagaacgca tcgtttatga tcaaacaatc agcgagcggc aagtggacgg
    48901 aaagcagatt gactttttga cttttatctg ttgcggaatg atttgtgatc atcgtttttt
    48961 gcaaaatctg tataaaaact atccaaataa aaagcaatta acgactcggc cgtttgccgg
    49021 gatgatcgaa gaaatgatct atttttatca attagaggca gaaaacctgt caaattatgg
    49081 taaactgttt gaacaagctt tttatctgac cgaaaatggc catctacttt ctgctagtat
    49141 gaaaaaattt ttaggaatga tgtttcattt cgtgtggaat ctccaagcat atccttttga
    49201 tttccaacta aaaaactttt ttgtgtggct ggacttgctt ggctatccaa tccactggct
    49261 ggaacagatt tcaaagatgg agtatttgga aaaagttctt tttttggcag aaaaaagcaa
    49321 aaaaatactt gttttagaaa aaataggtat aatagacgga taccaacgtc caaacgaaaa
    49381 agaattattg agaacaatga cggtatttca acaaatcgtc agtgagataa gagggaattg
    49441 aaatgaatac aaatagcgaa aaaatgttgc aagctttatc agatgaagac ttgtcacaag
    49501 cacaaatctt tttagaaaaa gcattaaaag aagacccagc agatatatta gctgaactag
    49561 gcgaagaatt gctggctatt ggctttttga tcgaagcaaa acagattttt gaacaactga
    49621 tcaaccaata cccagaaaat gacggactga acatcccgtt agcagaaatt gcaatcgaag
    49681 ataatgagat cgacttagct tttgaatacc tggaaaaagt ggataagcaa agtgattact
    49741 atccgcaaag cttattggcg atagctgatt tgtaccaagt tatcggaatc cctgaagtga
    49801 gtgaagcaaa attgaaagaa gcagctgggt tgcttcctga tgaaccattg atccagtttg
    49861 cgctagctga attgtatttt tcagttgatc gtttcaaaga ggcagcagtg gtttatgaaa
    49921 cattgttggc aagcgggata aatgaaattt caggaatctc gatgcaggaa cgtttaggac
    49981 aatccttaag tatgcaagga gaattcgaag cagctatcgc acctttagaa gaagcactga
    50041 aagaagaaag gacagatgat cgactgttcc agctagcttt tacgaatctt caattgaaag
    50101 aaaatcagca ggcaatcaac tatctggaag aacttcgaga agttaatccg cattatcaat
    50161 cgttatatct atatctagga caagctcttc aagaagaaga aatgattgaa gaagcacaaa
    50221 cagtgttaga agaaggaatc aaagaaaatc cttaccaagt cgaactttat cacctggcct
    50281 cggaaaatgc tttccgtctg catgatagac agcgagcaga agaactttta ctgaaagctt
    50341 tagaagtagg agacaaacaa gatgaaacat tacttacttt aagcaatctt tacttggatg
    50401 atgaaagata tgaagatgtg atacaagtga tcaatcagat ggaagaaact gctaatcctt
    50461 atgcagaatg gaacctagcc catgcttata atgaattaga agactttacc gttgcagctg
    50521 tacactatga acaggcttat catgaactat cacatgaacc agatttcttg aaagagtatg
    50581 cctttttctt aagagaagaa gggcagctga aaagagcgca agaattattg acttattatt
    50641 taacacttga accaggagat atggaggcac tgtcattatt agatgaccta acagagaggt
    50701 gagaaaaatg ctaattgatg tacgggaaaa gaagaatttt ttaacgtgga tggtcaatca
    50761 tgtatctttt agccgaagag aagttttttg gatcttgaat tatcttgcca atcatgaagc
    50821 gattttaagc aatgtccact ttgttgaagg ggcgaatttt gcggatcgag gactgcagat
    50881 cagtgatcta tctgttgaag gggaacccat gaaattgttt ttgcagggaa aagaatttac
    50941 ggacacagac cagatttttc atgaaatccg tttgaattgg aaaaagcctt tatatgtgga
    51001 atgtatattc gaaaatgctt ggcagacaag agaatatcta tctatcttag aagacaatcc
    51061 attttcttct tgggattctt cagtcagtga agaagatact gaattaattg aacgatattt
    51121 tcaagaaaaa gaagaagagg caaaactaaa aatgctttat gctcaaatcg atcaagcact
    51181 agaagatgga gatagggatg cttttttgaa attatctgat gaagttaacc gaaagatcct
    51241 tttgcagaca caaaagaaaa gtaaccgggt aagtgaacat aacaattagt taaaaatagt
    51301 atagtactaa aaaagacaaa gatcagctgg atgtggaatc
//
LOCUS       DAPBAB010000016        47701 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPBAB010000016 DAPBAB010000000
VERSION     DAPBAB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.016"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            102..545
                     /locus_tag="RD444_001123"
     CDS             102..545
                     /locus_tag="RD444_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7548510.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(551..1735)
                     /locus_tag="RD444_001124"
     CDS             complement(551..1735)
                     /locus_tag="RD444_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDT7548511.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2003..2701
                     /locus_tag="RD444_001125"
     CDS             2003..2701
                     /locus_tag="RD444_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDT7548512.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2708..3385
                     /gene="nth"
                     /locus_tag="RD444_001126"
     CDS             2708..3385
                     /gene="nth"
                     /locus_tag="RD444_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDT7548513.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3511..4044)
                     /locus_tag="RD444_001127"
     CDS             complement(3511..4044)
                     /locus_tag="RD444_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548514.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4341..6722)
                     /locus_tag="RD444_001128"
     CDS             complement(4341..6722)
                     /locus_tag="RD444_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDT7548515.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6712..7359)
                     /gene="recU"
                     /locus_tag="RD444_001129"
     CDS             complement(6712..7359)
                     /gene="recU"
                     /locus_tag="RD444_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDT7548516.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7423..7962
                     /locus_tag="RD444_001130"
     CDS             7423..7962
                     /locus_tag="RD444_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDT7548517.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8052..8471
                     /gene="gpsB"
                     /locus_tag="RD444_001131"
     CDS             8052..8471
                     /gene="gpsB"
                     /locus_tag="RD444_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDT7548518.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8493..8860
                     /gene="rnpB"
                     /locus_tag="RD444_001132"
     ncRNA           8493..8860
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RD444_001132"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9069..10235
                     /locus_tag="RD444_001133"
     CDS             9069..10235
                     /locus_tag="RD444_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDT7548519.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10281..11777
                     /locus_tag="RD444_001134"
     CDS             10281..11777
                     /locus_tag="RD444_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDT7548520.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11858..12541
                     /locus_tag="RD444_001135"
     CDS             11858..12541
                     /locus_tag="RD444_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7548521.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13186..14376
                     /locus_tag="RD444_001136"
     CDS             13186..14376
                     /locus_tag="RD444_001136"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDT7548522.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14478..15431
                     /locus_tag="RD444_001137"
     CDS             14478..15431
                     /locus_tag="RD444_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDT7548523.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15500..16849
                     /locus_tag="RD444_001138"
     CDS             15500..16849
                     /locus_tag="RD444_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7548524.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17032..17355)
                     /locus_tag="RD444_001139"
     CDS             complement(17032..17355)
                     /locus_tag="RD444_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548525.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17484..18647
                     /locus_tag="RD444_001140"
     CDS             17484..18647
                     /locus_tag="RD444_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDT7548526.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18717..20066
                     /locus_tag="RD444_001141"
     CDS             18717..20066
                     /locus_tag="RD444_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7548527.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20111..20542)
                     /locus_tag="RD444_001142"
     CDS             complement(20111..20542)
                     /locus_tag="RD444_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7548528.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20930..23572
                     /gene="alaS"
                     /locus_tag="RD444_001143"
     CDS             20930..23572
                     /gene="alaS"
                     /locus_tag="RD444_001143"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDT7548529.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23772..24368
                     /locus_tag="RD444_001144"
     CDS             23772..24368
                     /locus_tag="RD444_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7548530.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24490..25638
                     /locus_tag="RD444_001145"
     CDS             24490..25638
                     /locus_tag="RD444_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7548531.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25676..26089)
                     /locus_tag="RD444_001146"
     CDS             complement(25676..26089)
                     /locus_tag="RD444_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDT7548532.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26168..26599
                     /locus_tag="RD444_001147"
     CDS             26168..26599
                     /locus_tag="RD444_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDT7548533.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26698..26931)
                     /locus_tag="RD444_001148"
     CDS             complement(26698..26931)
                     /locus_tag="RD444_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDT7548534.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27114..28784
                     /locus_tag="RD444_001149"
     CDS             27114..28784
                     /locus_tag="RD444_001149"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDT7548535.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28931..29317
                     /locus_tag="RD444_001150"
     CDS             28931..29317
                     /locus_tag="RD444_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548536.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29304..30002
                     /locus_tag="RD444_001151"
     CDS             29304..30002
                     /locus_tag="RD444_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7548537.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30007..30471
                     /locus_tag="RD444_001152"
     CDS             30007..30471
                     /locus_tag="RD444_001152"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDT7548538.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30468..31379
                     /locus_tag="RD444_001153"
     CDS             30468..31379
                     /locus_tag="RD444_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7548539.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31614..32153
                     /gene="pyrR"
                     /locus_tag="RD444_001154"
     CDS             31614..32153
                     /gene="pyrR"
                     /locus_tag="RD444_001154"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDT7548540.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32167..33450
                     /locus_tag="RD444_001155"
     CDS             32167..33450
                     /locus_tag="RD444_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDT7548541.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33463..34389
                     /locus_tag="RD444_001156"
     CDS             33463..34389
                     /locus_tag="RD444_001156"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDT7548542.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34408..35688
                     /locus_tag="RD444_001157"
     CDS             34408..35688
                     /locus_tag="RD444_001157"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDT7548543.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35693..36769
                     /gene="carA"
                     /locus_tag="RD444_001158"
     CDS             35693..36769
                     /gene="carA"
                     /locus_tag="RD444_001158"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDT7548544.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36774..39959
                     /gene="carB"
                     /locus_tag="RD444_001159"
     CDS             36774..39959
                     /gene="carB"
                     /locus_tag="RD444_001159"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDT7548545.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40013..40789
                     /locus_tag="RD444_001160"
     CDS             40013..40789
                     /locus_tag="RD444_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDT7548546.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40786..41718
                     /locus_tag="RD444_001161"
     CDS             40786..41718
                     /locus_tag="RD444_001161"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDT7548547.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41715..42431
                     /gene="pyrF"
                     /locus_tag="RD444_001162"
     CDS             41715..42431
                     /gene="pyrF"
                     /locus_tag="RD444_001162"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDT7548548.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42438..43067
                     /locus_tag="RD444_001163"
     CDS             42438..43067
                     /locus_tag="RD444_001163"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDT7548549.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43055..43369
                     /locus_tag="RD444_001164"
     CDS             43055..43369
                     /locus_tag="RD444_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548550.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43407..44048
                     /locus_tag="RD444_001165"
     CDS             43407..44048
                     /locus_tag="RD444_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7548551.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44091..44783
                     /locus_tag="RD444_001166"
     CDS             44091..44783
                     /locus_tag="RD444_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDT7548552.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            44780..45682
                     /locus_tag="RD444_001167"
     CDS             44780..45682
                     /locus_tag="RD444_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7548553.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45840..47546)
                     /gene="efbA"
                     /locus_tag="RD444_001168"
     CDS             complement(45840..47546)
                     /gene="efbA"
                     /locus_tag="RD444_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDT7548554.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15520 a         8350 c        10270 g        13561 t
ORIGIN      
        1 ttttttcttg gtttcagatt gggttttcct acaaaatatt tttatctttg ttgaatagga
       61 aacaaaaaaa caatataatg aaagaaaaag aaggtgtaga aatggataaa agacaactgc
      121 taatagaaga atatctggat ttagctaatg agattattca tcgtaataaa ccagaaatgg
      181 aaaaagaatt tgcaggattt actctaaatg aactagaagt gattgaacat atcgggaaaa
      241 tccctgatcc taacgtgaca aaattagcga atgctagtta catgactaga ggagccatca
      301 gtaagctaac aaaaaaactg atagctagag ggatcataaa cacttatcaa agcgaagaga
      361 ataaaaaaga aatctatttt caattgacgc caatcggaca aaaaataagg aatagacacg
      421 agcaactcca tcaaaaatgg gtacagcgag atgctactgt atttgaaaat atgtcaaaag
      481 aagaatttga cacagtattt cgttttatcg gtcgatacag agaatttata cgaagtagca
      541 aatgaagcta ctatttttta ttgtagtgat aataggcaaa aatcataagc aaaaatccga
      601 ataccgtcgg aagaaatggt gctgcagcat aaagctgtcc tgcgattagc gggccgatga
      661 cgcgcgatag agattgtata ctttgggtac cgcccattac aagaccttgc tggttatctg
      721 agacagaatt ggatagttgg ccattgaata caggagtaaa aatagaatca ccgaaaccat
      781 aacagataat cccaaataaa aacaaaatcg gcagtagaag aattccagat aatgtgatga
      841 atagataggc gaggatttca ctgaacatcc ctattcttgt gatctgctgt tctgaaaatt
      901 ttttgagcaa tcgaggcata atgaacaatt gcgagacagt atccaagatt ccaatcaaag
      961 agaagcttaa accaaccaac ccagccttcc actgaaaggc gtcaatcgaa aattgagaaa
     1021 agatagattg aagagagcca gctgccaacc aaacagcaaa acctgctgtc aataaaggaa
     1081 taacggatga aatcttgaat aattgttgta attggtgaaa aggtcttaca tgggagaaac
     1141 ttaaatctac tgaacgtttt tttattggaa ggctttctag cataaatgtg tagccataaa
     1201 cggcatttaa gaaagtaaag agtgctccaa taaatatagg aacagaattc cccaactcag
     1261 ctaatagacc ccctataata ggacccagcg ttgttcctat accgactaaa gcacccatcc
     1321 atccaaaaac tttggttctt tcattaggtt ccgtgatgtc ggcaaagtaa gcatataagg
     1381 tggagatttc tcctgccgtc aatccttcaa tgatacgacc aaggaacaac atccatatcg
     1441 aatttcctaa accaaaaagc aaatatccga tacttgatcc taataaactg ataataagga
     1501 tcggatgacg cccaaaatga tcacttaagc taccgagaat gggggcagat aagaaggcag
     1561 ctagagcata aacagacatg agcaaagtga tatagaaagc ttgctgatgt atggagtgag
     1621 aataaggcag cgttaaaaac ggaagtacag gactgacgat agtaaatcct aatccagtga
     1681 ataagacaga gacgtatcca aaaactttag aatgtttatt tgtgaaactg tacatgtaaa
     1741 aactccttta tgcaataatg ttaacttgga aacaaaaata gtgtatagta attttgtttc
     1801 cttgtcaaca ttctaaggaa cagcgaaatc tacttaattt atttattccg aaaagtgaag
     1861 atacgcatca aaaacatgaa caaacaaaaa tcaagtagac gacattgtaa atctgaccga
     1921 tcagtgaagt aaatagaaaa gaaacctgtt cttgcttccc atgttttttt tacgtatact
     1981 gtgattgagc ggaggagaaa tcatgttgaa tttagaaaat tacttaagcg ctggacaaac
     2041 agtcatatct aatcttttac ttaaaaatta tcataggatc ggtctacaat ctgaagaatt
     2101 tttatttgtt ctacagttgc atatggcaca gcttgaagga gatactttcc ctgatttgca
     2161 gatgattgct atggatatgg gaatcaaaca agatcagatt tttcaaatac tggatcgtct
     2221 cgtaacaaat ggatttatca agatagaaac gacgattgtc aacggtaaaa aagcagatcg
     2281 atataatctt taccccatct atgatctatt aggtgagtat ttaaaaacac aagaaaaaaa
     2341 atacgaagaa aaaacacaag aaaaagaaat ccagtcagtg tatcaattat ttgaacaaga
     2401 atttggacgc ccgctttctt cgatcgagtt ccagcggatc ggacaatggc tagaagaaga
     2461 ccattatcag ccggaaattt tgaagctagc actgcgggaa gccgtattaa atcaggctta
     2521 tagttttaat tacgttgacc gcattttgct atcgtgggaa agaaaaaatc tacgtacaaa
     2581 acaacaagtg gaagaagacc aaaaacgaag aaaacagcag cttcttcaaa gagaagtaga
     2641 acagtcagct caacaagaag aattgccgaa agtctcactg aaaaattggt tggaggagta
     2701 gcggaatatg ttaagcaaac aaaaaacaat ggaagctcta gaaacgatgt atggaatgtt
     2761 tcccgaagca catggagaac tcaagcacaa taatccattt gagttattga ttgcagtgat
     2821 cttaagtgcc caggcgacag atgtatcagt gaataaagca acgccagatt tgtttgcctc
     2881 ttttccgaca cccgatgcgt tagcagaagc ttcaatcgat gaaatcattc ttaaaataaa
     2941 aacgattggt ttgtatcgaa ataaagcaaa aaacatcaaa gcgtgtgcac agcagctgat
     3001 tgaacgtttc gatgggcaag tgccaacgtc aagagaagaa ttgatgtctc ttcctggtgt
     3061 aggcagaaag acagcaaatg tcgtattggg ggatgcattc gggataccag caatcgcagt
     3121 agatacccac gtggaacgtg tctcaaaacg cctgaggatc tgcaaactag atgctacggt
     3181 catggaagta gaagaaacac tgatgcggaa agtaccacaa gaattatggg taaagacaca
     3241 tcatacactg attttttttg gtagatatca ttgcacggca agaaatccca agtgcgaggt
     3301 atgtcctttg ctatcaatat gtcaagatgg gaaaaatcgg atgcgtctaa aagaaaaggc
     3361 gctaaaaaag aaaccacgcc tgtagacgtg gtttctttaa ttgcaaataa atgtatgaat
     3421 tatcttgtta ttatggagaa aagctgtttc gtttatcttt tttgcatgaa aaagtaaatg
     3481 tacatactca gtagctagga tggtaactta ttacaattta ttgcgataaa aataaaagtt
     3541 gttattttta ttgggaaaat acccgatgta gaaaataatg ccttccaata tacctataaa
     3601 ataagtccag tattccaaag gattatataa ataagtgacg tatctatcta aaggtgtaat
     3661 aaaaatgata agtgataata aaaaaatacc atttataact aaacataaac taatggattt
     3721 gggcggtttc aggctcatca gaaaaatata gattctggtg aacaacaacc ataaaaaagt
     3781 gagaatcatt gcatgagagc ttctttccat aaattttcct ttaagagtag tgcttaaaag
     3841 tacagaaaaa ataataaatg caaggatagg agcaattaaa gtaaatttat ttaataataa
     3901 acaaaatttt tcagcttttt tattcttgga ataacgaggt attgttttcg ataaaaaaag
     3961 atggtgcaga caaattatga taattaagca tactatcaga taactcaaaa tgaagccttc
     4021 tttcaaaaaa ttattcaaaa ccatatattg tattttttat ttgataataa caatgggatt
     4081 attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa ttataaacaa
     4141 gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat ttttaagcta
     4201 aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt aatacagctt
     4261 ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa accacgcttg
     4321 tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct ggatggtgta
     4381 tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc aggaggagca
     4441 gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt tgaagacgct
     4501 ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga ttcgctttga
     4561 ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg agtctggaca
     4621 tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc tgtatattga
     4681 tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc ccagtctgta
     4741 tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga ggctacgtgt
     4801 gacgattctg aagtgacagg tgtcattttt ttattgtaac ctgtccacac ggaaatcgaa
     4861 taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga aataccaagt
     4921 tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg atataaccca
     4981 gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat gtctgtgatc
     5041 atataagctg tttctggaga catggctgtt tttccatctg gctcataagt ttcttctgtt
     5101 ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc tccattggcg
     5161 aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt cccatcttgt
     5221 tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc gatcccaaga
     5281 ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt gactgctgga
     5341 acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata ttgattatcc
     5401 cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt aatagttttt
     5461 ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta
     5521 gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac gtcttctgcg
     5581 atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat aagggtggag
     5641 gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac gatatcatat
     5701 agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc tgtgtagaca
     5761 ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt atcgacgatc
     5821 ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg tacattaaca
     5881 gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata caaaacagta
     5941 tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga aggggcttga
     6001 ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt cttaccaaag
     6061 tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac tttatttacg
     6121 tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc catccacgct
     6181 tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa ggaaagtttg
     6241 atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa gttagagagg
     6301 gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct
     6361 accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat tttttcacgc
     6421 ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt ggaagatacg
     6481 gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt
     6541 cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag gatcttgata
     6601 aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga attcttcttc
     6661 acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc
     6721 attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg cgtccaaata
     6781 aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga tcaaagataa
     6841 aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca aatgaacact
     6901 attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc agaccccatt
     6961 ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt taaagggaaa
     7021 agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc gatagacgcc
     7081 attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag cagcacttct
     7141 cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga taaccgccaa
     7201 cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc gcatcccccg
     7261 attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg gagaggcatt
     7321 cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca ctcctaggcc
     7381 acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa gtcaaaagta
     7441 atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa
     7501 atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga agaagggctg
     7561 gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca aatagtagcg
     7621 gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt ttctgatttt
     7681 ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca attagcagat
     7741 tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa gaatcacact
     7801 cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga attccctgga
     7861 aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc ctacgaaatt
     7921 cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt
     7981 tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta
     8041 gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa caagaattca
     8101 aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac aatatcatca
     8161 aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac gatcgattga
     8221 gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa
     8281 cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc ttgtcaaatc
     8341 ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac
     8401 aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat gaaaaaacac
     8461 gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg tctgacttga
     8521 tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc
     8581 gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta aggcttcggc
     8641 tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa cccatccggt
     8701 ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg
     8761 gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg ctttgttttt
     8821 tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac atcagggaat
     8881 taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa agctattttt
     8941 caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc ggttattcgg
     9001 aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta taaataagga
     9061 gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca agcgggctag
     9121 aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt gagaacggac
     9181 gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg ttacgtacag
     9241 cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat gaattgtttg
     9301 aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct tttcctgtag
     9361 ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg atcacaaaaa
     9421 aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta ccactgacgg
     9481 aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg atggtaactc
     9541 tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa ggtggagcac
     9601 cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga aaagaccgtc
     9661 cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc ttaataggcc
     9721 ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat tggttcagtc
     9781 aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat gatattgaat
     9841 tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa gccaatgcag
     9901 aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa
     9961 cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt ctaggagaag
    10021 aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca ttaaaaacat
    10081 ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga caaaaagcaa
    10141 ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag tattggggaa
    10201 ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt cattcaattg
    10261 gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta aaagaaatca
    10321 atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt atgcctgaaa
    10381 aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat ttttcaaaaa
    10441 aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg gaagaattgt
    10501 cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg gaacatcgcg
    10561 taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac ggacaatggc
    10621 aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat caaaatattg
    10681 aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat tatgatgttt
    10741 tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta tttgatcagc
    10801 ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact gaacctgaaa
    10861 cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa
    10921 tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc cacccattta
    10981 tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa acagatttca
    11041 aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa caagacatcg
    11101 atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc catgaatcac
    11161 aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa aagcaatatc
    11221 ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac aaattttatc
    11281 gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc
    11341 ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg
    11401 ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga atacgaccaa
    11461 caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc tttggttatt
    11521 ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct ttgcagcaag
    11581 aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa aactggatgt
    11641 cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa
    11701 cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg tattttaatg
    11761 tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct aatctataga
    11821 tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac aagtgatcgc
    11881 tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg atcataccaa
    11941 aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag accgctttgt
    12001 gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta aaaatgagaa
    12061 cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag aggaacagat
    12121 ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct ctgaagcggc
    12181 cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag cattaggagc
    12241 aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga tttttgatcc
    12301 tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca cgacagtgat
    12361 caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg attacggata
    12421 tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat tttttctgga
    12481 atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata acaaaaaatg
    12541 atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt
    12601 tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa
    12661 ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa
    12721 ttggcaggag aacaccgttt attcggaaga ataggactgt gacaagactt ttgtcacagt
    12781 ccttttttcg aattcaacaa gtaaaacttt taagaaatat tcagagagta aaattaaaaa
    12841 ttgctgataa acgaagatgg aaattttatg tatttttatt ggtctatgca ataaataaat
    12901 taaatattca gaatacaatt gattttaacg aaagggcttc tatcaatggt tgtgttgtat
    12961 ttatctgccc aggtagagta aaatagatca ttatctaaaa ggtaattgtt catactatat
    13021 aagtcatttg aaagtaaaaa gatactgtaa ataaaaaatg tggttaaata attctcataa
    13081 tcagtgtaag cgttatcaaa aatatagcat acttgctttg tagatgaatg acacgatata
    13141 tgtttggtat gttctgcaag acagatgaat atggaggtgg agacaatgtc ttccaaagaa
    13201 ctaacaatgt atttagagaa agaattggat caactgaaag aaaaggggtt atacaatacg
    13261 atcgacatac tagaaagtga aaatggtgct tgtatcatcg tagacggaaa aaagatgatc
    13321 aatctagcat ccaacaatta tcttggattt gccaatcgcg aagaattgaa aaaagcatgt
    13381 attgaagcaa ccgaaactta tggtgtagga gcaggagcag tccgaacgat caatggttcg
    13441 ctgaaaatcc atcagcaatt agaagaaaaa atcgcagaat tcaaagggac agaagcagca
    13501 atcgcctttc aatctggatt caactgcaat atgggcgcta tctctgcagt aatgacaaaa
    13561 gaagatgcta tcttatcgga tgaattaaac catgcatcta ttattgacgg ttgccgctta
    13621 tccggagcaa aaatcatccg aattaaacat caagatatga aagaccttga gaaaaaagca
    13681 aaggaagcga tagaaagtaa gaaatataaa aaaatcatgt atatcacaga tggtgttttc
    13741 tccatggatg gagacattgc acgattgccg gaaattattc cgattgttga aaaatatgga
    13801 ctgatcacct acgtcgatga tgcgcacggc tcaggcgtaa ctggaaaagg tgcaggaacc
    13861 gtcaagcatt ttggattaag cgacaaaatc gatatgcaaa tgggcacgtt atcgaaagct
    13921 ataggtgttg ttgggggtta tgtagctgga tcgaaaacac taatcgactg gttgaaagct
    13981 agaagtcggc cattcctatt ttctacttcg ctaactccag gtgcagctgc agctgcattg
    14041 gcttcgatta ctttgatgca agaacatccg gaacttgtcg aaaaagtttg ggaaaatgct
    14101 aattatttta aagaagaatt gaaaaaagta ggctataaca tcggtatgtc tgaaacacct
    14161 attactccag tcattttggg agacgaaaaa gtgactcaga ctttctctaa gaagctgatc
    14221 gaacatggga tttatgctaa accaatcgtt tatccaactg tgcctcttgg gacaggcagg
    14281 atcagaaata tgccgactgc ggaacatacg agggaaatgc ttgatgaagc agtagcagtt
    14341 tatcaaaaaa taggaaaaga aatggaaatt atttaactaa atttagtttt ttcgtctatt
    14401 taaaattaga gaacatgttc acgcatatat tgttaatcat ctgaagatag tagtaaaaaa
    14461 gatcagggag gatcatcatg aaaaaagtat tggtgacagg atgtttagga caaattggtt
    14521 cagagttgat cacacggcta cgtcaagaac taggcagtga aaatgtcatt gcgacagata
    14581 tacgcatgcc tgaagtcatt gagaaaaacg atatctttga gatcctagat gtaatggatt
    14641 acgaaaaaat gcgcagcata gcagaacaat atcaagtgga cacattgatt catttggctg
    14701 ctcttttgtc agctgttgca gaagcaaaac cacaattagc ctggaatttg aacgtgactg
    14761 ggctggtcaa tgcattggaa gtcgctagag aattccagct gaaatttttt acgccaagtt
    14821 caatcggcgc gtttggtcca aatactccaa aagatcatac cccacaagat actcttcagc
    14881 gtcctacaac catgtatggg gtaacgaagg tagctggaga actactatgt gattattatt
    14941 atgtgaaata tggcgtagat actcgtggtg tccgtttccc tggattgatt tcttatgcaa
    15001 ccttgccagg aggcggtacg acagactacg cagtcgatat ttattattca gcaatcaaag
    15061 aaggaaagta cactagtttc attgatcaag gtacatcaat ggatatgatg tatatgccag
    15121 atgcgatcga tgccattata aaactcatga atgcagatcc tgatcgattg gttcatcgaa
    15181 atgccttcaa tatatcggcg atgtcttttg acccggaaga gatcaaagca tctattcaaa
    15241 aaatcatacc agattttgaa atggattatg atgtggatcc tgtacgtcag tcaatagcag
    15301 aatcttggcc aaattcacta gatgtttctt gtgcgcaaga ggaatggggc ttttcgccga
    15361 aatacgactt agatgtgatg acaaaggata tgttggaaaa actgaaagaa aaattggtag
    15421 agacggtata aaacaagaac cacatccaaa aagtttttta tacttttggg attggctatg
    15481 gtatgaaagg ggacaaggga tggaagaata tcaagccacg ccagtaaaag aagtaaaaat
    15541 agaaaatgaa ttaaaacgta cgatgggatt ttttactgct ttatcgactg ttatgggcac
    15601 agtgatcggt gcaggggtat tttttaaagc cgcaagcgta gcagaagtta caggatccgc
    15661 cagtttgcat atgttttctt ggtttctcgg tggtatgatt tctgtctgtg caggactaac
    15721 aggagctgaa ctggcagctg ctattccaga aacaggaggg atgattaaat atatcgaacg
    15781 gatttacgga aatacagcag cttttttatt gggctgggcg caagtcgtga tttattttcc
    15841 agcaaatgta gcagccttgt cgatcatttt cggtacacag ttcgtcaatc tatttggtct
    15901 ttcccagtca atgattgttc ccgtagctgt tacagctgct gtatcgatcc tgttgatcaa
    15961 ttttttaggt tctaaagctg gaggggcttt tcagtcgatt acgcttgtct gcaagcttat
    16021 tcctttgttt gtgatcgtca ttttcggttt gttccgacaa gaaggagtag acttccaact
    16081 gtttccgatc caagcaggag aaaatctttc atttttctca gccttgggag cgggcttgct
    16141 agcaacgatg ttcgcatacg acggttggat acacgtagga aatatttcgg gagaattgaa
    16201 aaagccggcg aaagatttgc caaaagcaat ttctttagga atcatcggaa tcatgatcgt
    16261 ttatttatta gtaaatgcgg tgttcttaag aaccgcatct atcgacggag tcgcaggaaa
    16321 cagcaatgcg gcaagtgacg ttgcaaaaat gatttttggc ggcttcggtg gaagactggt
    16381 cacagttggt atcttgattt ctgtttatgg aaccatcaat ggctataccc ttactggaat
    16441 gcgtctccct tatgttattg caaaagaaaa caacctgcct tttagcaagc tctttgctaa
    16501 acttcatgat aaaactaaag ttcctgtagc cgcaggtatc ttagagctag tcatcgctat
    16561 tggcatgatg atgatcggtg gttttgatac actaacggat atgctgatct ttgtcatttg
    16621 gattttttat acgatggtct ttgttggtgt cattcttctt cgtaaaaaag aacctgattt
    16681 gtttcgacca tacaaagttc caatgtatcc gtttattcca ctagtagcaa tcattggagg
    16741 gacttttatc gtatcaagca cgcttatcac tcagaccttt ttagcttcga tggggatagt
    16801 attgacactt gctggtatcc cgatttatct ctatttaaaa cgtagatagc taaaacattc
    16861 gaattttatt agaactgcac ttctctgcca aacaaaatat tttatttaaa tgaggagtac
    16921 gtggaagaaa tgtcaaaagt actcgttaat tttgatcacg taaatgtcgt tttgtctacg
    16981 atgaaatagc aaagtaaaag acagaaatcg ggctcaaccg atttctgtct tttactttga
    17041 tttgcgtaac tgaactattc ggcgtacgcc gcgataaata tttaataaaa ccaagcaacc
    17101 agaaatgaaa atgggaagcc aaatcagata gacagggtag ccaagcactt tagacaaagc
    17161 atgtggagtc attttatcca tttcaagaag tgcttgctgc caagtctgaa gattgaagac
    17221 tagcatgaaa cccgaaataa tcaggagaat aaatcctaaa taatgaattg tagttctttt
    17281 tcttacataa gcgatcgtag cataaaggaa cagtaaaaaa ataggtacgc caaaaacaaa
    17341 gtacatattc agcatctcga cacccccttt gtgtttatct tactttactt tgaaagaaat
    17401 gagaaataat agcctcgtta cttgcttgaa acagcaaagg actgctaaga taagagagat
    17461 acaaagaatc aaggaggagt catatggcaa gttatatcga accgattcgt tttgcgctga
    17521 ttgtttttcc atttttagcc ttagcgatct catctgtttt ttttatttat gaatatcgaa
    17581 agtatggaac atttctagta acaagggcag ttattttgta ttctttcgtt ttttatttat
    17641 tgtgtgcgta ttttttggtg atcttaccac ttccggcaaa ggagacggtt gcccaaatga
    17701 cagggccaaa gatggagctg catttatttg cttcatggca acatttcaga gacaacacgg
    17761 tattggtctt gtctgaacct agtacctacc tgccggcaat gaagcagagt gtatttttgg
    17821 aaccagcttt taatgtttta ttactcatac catttggcat ttatttaaga tattatttcc
    17881 ggctttcttt tatgaaaacg atgattgcta gtttctgcct ttctctattt tttgaattga
    17941 cgcagctaag tggactttat tttatctacc caagaccata ccgcttattt gatgtgaatg
    18001 atttagtgac aaatacattg ggtggaatca ttggttttgt cgttacgccg ttatttacat
    18061 ttatgcttcc tacaagaaga cgaatggatg aactttctta tgaaaaagga aagactgtct
    18121 cattgactcg acgcttggtc gcttatttga tcgactggac atttttatct cttattaatt
    18181 tggtagcggc gattttcttt aaaattgcgt tcgattcagg ggcagttact tctcatatct
    18241 ggtggctttt attagaagta ggggtatact ttatgattgt accgtttata accaatggat
    18301 ggacacttgg caaaaaaatt gtgcgtatcc gtttggtgga agaagaccgc gaacgaattt
    18361 ctttaaaagc attgatgatc cgctacgggt atctgtactt tcttttttat ggtttaagct
    18421 ggttggggca gttgcttcag caaatcatga attcgacaac gaatacggtg ttgcagatga
    18481 gtgcagtatt catttctctc ggtgtcttgt tactacaagg tatttttatt ttaaatatct
    18541 tgctatcatt tttgagaaaa aaacggattc tgttttatga aaaagctagt cacacatata
    18601 ctgttagcac gattggccaa acaaaggatg atgattctcg aaattagctg agcaaaaacg
    18661 ctttgtctgt gttataatgc caaagtcgtg ataatttaaa gagaagaggt gcacgaatga
    18721 ataattttga tatattccgc ttcaaaccgt ttatcaatga agcgttaacc gcaaagggct
    18781 tcaaacagcc cacagaggtc caggaacgtt tgattccttt aattgcaaaa ggaaaaaata
    18841 tcattgggca atctcaaact ggtacaggaa aaacacatac gtttttgttg cctttgtttg
    18901 aaaaaatcaa tgcagaaaaa gaggaagtac aagttgttat tacagctcca agtagggaac
    18961 tagctgcaca gatctatgaa gcagctacac aaatcgctaa attttctaca ccggagatcc
    19021 gtgtctcgaa ttttgtcggc ggtacagaca aacagcgtca attaaatcgt ctgcatcacc
    19081 aacagccaca ggttgtagta ggcacgcctg gtagaatatt agacatgatg aatgaacaag
    19141 cgctgaagat tcacaccgca tttgcatttg ttgtcgatga agcggatatg acattggata
    19201 tgggtttttt aaatgaagta gaccaaattg caagtcgatt accagaaaag ctgcagtttt
    19261 tagttttttc tgccactatt ccagaaaaac ttcgtccatt cttacgaaaa tacatggaaa
    19321 atccaatcat tgaagaaatc aaaccaaaag cagttatttc tgaagacatt gaaaattggc
    19381 tgatttcaac aaaaggtaaa gatgagaatc aaatgattta tcaactgctg acaattggtc
    19441 atccttattt agcaatcgta tttgccaata caaaacagca tgtggatgaa attgccgatt
    19501 acttgaaaaa ccaaggcttg aaagtagcaa agattcatgg agacatcacc ccaagagaga
    19561 gaaaacgagt gatgcgacaa gtacagaatc tggagtatca atatgttgta gcgactgatt
    19621 tagctgctag agggatcgat atcgaaggtg tctctcatgt tattaatgca gaaattccag
    19681 aagatctaga cttctttatc catcgtgtcg gacgtactgg acgtaatggc ttaaaaggaa
    19741 cggccattac gttatattcg ccaaatgatg agcaagccat tgatgcgata gaaaaattgg
    19801 gcgtctcatt tgaaccaaaa gaaatcaaaa acggggaaat cgtcaaaaca tacgatcgta
    19861 atagacgtgc caaacgagaa aaatcaaaag atgtattaga tcccacgctg attggtcttg
    19921 tgaagaagaa aaagaaaaag atcaagcctg gttataagaa aaaaatcgat tgggcaattg
    19981 cagaaaaaaa taaaaaagaa cgcaaaatcg aacgcagaca gcaaagccgt actgctcgta
    20041 aaaataagaa aaattcacat aaataaaaaa caagaggttg tgacaaaagg cgtgtcacaa
    20101 tctcttgttt ttaagctaga ctttttttca ttccgataca aagtacttca tcttcaataa
    20161 aaggttctgt gcaagtatga tacccttgtt tttcataaaa atctacagcc gtcatttcag
    20221 ctactacata actagtctga caaccttctg aaaccgcccg tttttcagca gcttcaagca
    20281 actgaccgcc taaccctttt cctcggtatt cttttgctac gcaaaatcgt tccggcttga
    20341 agcaagtagg tgttttcttt tcataacgca aagtagctac tggtcttttt tgatcaagaa
    20401 atagataata ggtttgttgc gggatatctt ttccgtcgaa ttctagttct gcaggaatcc
    20461 cttgttctaa tacaaaaact tgatatctca aaaagcagct agctgcgcgt atccattcat
    20521 ccgctccaac tatttttttc atcatattcc tccttgtcat gaatagcaga gatgtattga
    20581 ttcctttatt cttatcgtac caaaaatcgt ggagtatgac caaaaggaaa tgaaaataaa
    20641 aattgagata ttgacagtga tcaatagaat gcgtatacta attaagtacg aaaaggatat
    20701 gatttgtttt ttaagactca cagaaaattc tttcttggct gaaaaaagaa tagtcactaa
    20761 tcaaattgct ccctttttct attttggtag aaataccgac gcagctcagc gttatcgagt
    20821 ttaagaggta atgattacac atcattgcaa tcaaggtggt accgcgagtt ttcgtccttg
    20881 gctgtgatgg tgtttttttg tatttaattt atagaaagca ggtaatgaca tgaaacaatt
    20941 aagtagtgca caagttcgac aaatgtttct tgatttcttc caatctaaag gacatacgat
    21001 tgaacctagt gcgtctttga tccccgtgaa cgatccaacc ttattgtgga tcaactctgg
    21061 tgttgcaaca ttgaaaaaat attttgatgg ttcagttgtg ccagaaaatc caagaatcac
    21121 aaatgcccaa aaatctattc gaacaaatga tatcgaaaat gtcggtaaaa cagcacggca
    21181 ccatacgatg tttgagatgc tgggtaattt ttcgattggt gactatttca aaaatgaagc
    21241 catccactgg gcatgggaat ttttgactga tccaaaatgg atggcattcg atccagaaaa
    21301 actttatgta acagtttatc caaaagatac agaagcaaaa cgcatctggc atgaagaagt
    21361 tggcctgcca ctagatcaca ttattgatgt agaagacaac ttctgggata tcggtgcagg
    21421 cccatgtggt ccggatacgg aaattttcta tgaccgtgga gaagcttata acgatttagc
    21481 agaagacgat cctgaaaact atccaggagg agaaaatgaa cgttatttag aaatctggaa
    21541 cttagtattt tcagaattca accataagcc tgataacaca tttgaaccac ttcctcataa
    21601 aaacattgat acggggatgg gattggaacg catggtctca atcgttcaag atgcgtcaac
    21661 gaactttgag acagacttgt ttatgcctat tatccacgcc gtagaaaagc taagcaaaca
    21721 agtaaaatat ggtgaaaacc cagtaacaga tatttcttac aaagtaatcg cagatcatat
    21781 tcgcgccctt tcatttgcta ttggcgacgg tgcattgcca tcgaatgaag ggagagggta
    21841 tgtcttacgc cgtttgcttc gtcgtgctgt catgcatggg aaaaaattag gtattgaaca
    21901 agcattctta tatcgtttag tccctgttgt tggggaaatc atggtaagtt attatcctga
    21961 agtacttcaa caaaaagaat ttattgaaaa agttatccgt acagaagaag aacgtttcaa
    22021 tgaaacaatc aatgaaggtt tggaaatctt aaatcaagtg atccaagaag taaaaaacga
    22081 aaacgggacg actctagcag gaaaagacat cttcaaacta tacgacacat atggcttccc
    22141 ggtagaatta acggaagagg tggcagaaga tgaaggactg aaagttgatc atgctggttt
    22201 tgaaaaagaa atggatgcac aacgcgaacg tgctcgctct gcccgcagta ctgaaaaatc
    22261 aatgggggta caatctgctt tattgacaga tatcaaagta gaaagtaaat tcattggata
    22321 cacagaacaa gaatcagata gtcaattatt ggtcatcgtt aaaggtgagt ctcttttgaa
    22381 tgaagtcgca gaaggtgaag ctcaaatgat ctttgatcaa acacctttct atgcagaaat
    22441 gggtgggcaa gtagcggatc aaggatggat catgaatgaa gtaggcgaaa tcgtagcagt
    22501 tgttacagat gttcaaaaag cgcctaatgg tcaatttatg cacaccgtag aagtaaaagc
    22561 accaatgagt gaaggacaaa cctatcatct attgatcgat caatcgatgc gcaatcggat
    22621 catcaaaaac catacagcaa cccatctact gcacaaggca ttgaaagaag tacttggcga
    22681 gcatgcaaat caagctggtt ctttagtcgc tcctggacat ttacgattcg actttaccca
    22741 ctttggtcaa atcacttcag atgaattagc tgaaatggaa agaatcgtca acgaaaaaat
    22801 ctgggaagct atcccagttg aaacaattga aacagatatc gatacagcca aaaacatggg
    22861 tgcaatggct ttgtttggtg aaaaatacgg acatgacgtt cgagtagtaa atattggcgg
    22921 ctggtcaatc gaactgtgcg gaggcacaca tgtagcaaat acagaaaaca tcggaatctt
    22981 caaaatcgtt tctgaatcag gaattggtgc aggcgtgcga cgaatcgagg ctgtaacaag
    23041 taaagaagcg tacgaactac ttgaagaaga agaaaaacaa ctaaaacaag tagcaacaat
    23101 tgttaaatca cctcaattga aagaagttgt ttcaaaagcg gaacaattgc aacagcaagt
    23161 aagagagcta caaaaggaaa atgaacaatt agctagcaag ttagcgaatc aacaagctgg
    23221 tgatgtattc aaagatccag aaacgatcaa tggaaaaaca atcattgcag cacaagtaaa
    23281 agtgaaagat atgaatcagc tgcgtcaatt agctgaccaa tggaaacaaa aaaatatttc
    23341 agatattctt gttttagcaa acgaacaaga aggaaaagtc agtctgctag cggcaatgac
    23401 gaaagaatca aatgaagctg gactaaaagc tggcgacttg attaaagcaa tcgcaccgaa
    23461 agtaggcggt ggcggtggtg gacgtccaga catggcacaa gctggcggga aaaatcctgc
    23521 tggtattcca gatgcactta aagctgcgaa agaatgggtt gcgcaacaat agggcgttta
    23581 cgtttgacag aaaaacaaaa gttcaaacat tgaacgaaac atgattatgg gggcgaagac
    23641 aaacaagtct tgccctcata cttttttcaa ttggatggcg catctaatca aatggtcgga
    23701 ttcggatggt ctttagttga cgaaagtgac agaaattttt acgattagta taaataaaaa
    23761 ggggtgaaaa aatgaatagt agaaataaga cctttcgact agtcttacgt gcgatcttgt
    23821 tagcaatcat catcgtacaa gcgatggttc cttggctggg gtttattcca ttaggattca
    23881 ttagtttaac aattatccat atcacagtaa ttattgctgc agtagtatta ggcccaaaag
    23941 atggcatggt gattggcttg ttttggggaa tcgctacaat tgttcgtgcc tatgccatgc
    24001 caacaacgcc atttgataca ttagtgttca cgaatccgat tatctcggta gttcccagag
    24061 tacttgttgg cttagtagca ggcttggttt tccactggat ttatcaaaaa aataaatcaa
    24121 taacgatcag ctcagccttt gcaggggtac tgggttcatt ggtcaataca gtcttagtgt
    24181 taggattcat gggattattt tatacaggtg caacggcaga tgcctatggt gtagactctg
    24241 ctcttttatt taaaacctta gcaggggttg cagcaatcaa tgggatacca gaagcaatag
    24301 gtgcgggaat cataacacca ttactagcaa aagcgttatt tgcagcaaca ccgttgaaac
    24361 cagaataatg accatctttt aatagtaata ggacgcacga tagatagaaa aaggaagaga
    24421 ggagaggaaa acaatcgtcc tctccatctt tttacctgag caacaagcaa actagaaggg
    24481 gtaaaaaact tggctaagaa acaagaagaa aaaaagaaac aaataccgca acagcggaca
    24541 aaaaaaggag tactagcggg tatcttagga ttggttacga atctgatctt gtttgtctct
    24601 aagctaatga tcgggctttt ttcaggaagt gtctccatta tggctgatgc agtgaacagt
    24661 ctatcagata ctgcttcatc catattgact ttgatcggat ttaaaattgc tgcaaagcca
    24721 gcagatcagg aacatcctta tggacacgaa cgcttcgaat atatcagtgg gttatttgtt
    24781 tcgatcatca ttacttatgt aggatttcaa tttttagagt cttctgctga aaaaatcatc
    24841 catccagaaa atgtctcttt aacacccatc gtcttttttg tcctaatcat ttccatcttt
    24901 ttgaaatttg cacaaggcaa aatgtatcag gttattgcgc aatccattga ttctgaaaca
    24961 ttgagagcta cagcaacaga tagttataat gatgtcttta ctacactggc tgtattgctt
    25021 tcagcagcaa tcgaatgggt aactggatgg cgtattgatg gatatatagg ttttgcttta
    25081 gcattatata tcctttatag tggattgacg atgcttcgaa attttgtata cgaactatta
    25141 ggaagtcgcc caacgccagc agagattgaa gaaatggaag agcatttaaa tgcttgcgat
    25201 gatatacttg gctatcatga tttattggtg cataactatg gacccaataa acgctttgca
    25261 tcagtccaca tcgaagtaga tgaatcaatg aatttgaatg tggctcataa gattattgat
    25321 ctgatagaaa aagactttaa aaagtctttg gatgtggaac ttgtctgtca cttggatcca
    25381 atgcctgttg ataacgaaaa atactataat atcttgcggc agttaaagca aattcttctt
    25441 gaaacaggta cagggcttaa tattcatgat tttagagtaa ttcatcaagg aaaaacgctg
    25501 caatttgatg tagtggtccc tgagaaattc cagtttgttg atacagaact agaaagaatc
    25561 ttacgtagtc aaatagaaga aaaaatcgga gattaccaat tggaaattac gtttgatcac
    25621 aattatcttt tatattaaat gagaaactat aaaaagactc cttaaagttt ttaatttaaa
    25681 gagtcttttt ttcttttaat tgtttatgga gagcctgacg tgcaagatta tctgctcctt
    25741 tatttttgtt ttcgggcaac cattgcagga tcaataaaga aaattcggat agaagttctt
    25801 ggattctttg aaggtaggga aggaagagct cgttcttcgt tgcgtttttg tcgaccgtag
    25861 aaactagtgt tttgctgtct gaaaaacaga aaatcgtttc attctgtaag tcgtttttct
    25921 ttagaaattc taaagttttt aaaaagactt cgaactctgc ttgatggttt gtccctatag
    25981 aaagcgggat agatagctgt tcttgtttac cttgatagac aatcaagatt ccacctccac
    26041 taggaccagg gttgcctttt gtagaggcat caatgtatac ttttaacata aaaagagatt
    26101 ctcctttttg ttcaaactaa tgttatggta agattatatc attactgaat cggtgagagg
    26161 ttagagaatg aaaaaaaata ttcgatggca gcctgaactg gcacatacga tcatctattg
    26221 gtcattgacc ttgatcattc tgttttttag tctgattctt tctttagaaa acacaggtcc
    26281 ttattggaaa agcaattttg ttttaggtat ttttcttgtt tttctatttc taggtttgcg
    26341 gcgttctttt attttaaaat caaactctgt gattatacgt tatgctgctt tttggaaaga
    26401 ccgagaaata tctgtcggac agattcgatc tttgacgcca actaatactg gggtgcaaat
    26461 gatgttgcaa gatgagaaag ccccatctgt tttcatgatg cgaaaaaaag atcaagtagt
    26521 cctttttgat cattttgaaa aaaacggacc caaatcagaa tcaattcctt atccttatac
    26581 gaaaaaaaga aaaagctaat tttaaaaatc agaacctaaa aaaacgaatt tttacaggaa
    26641 aagaaaagag tgagacgtac ggttcatccc gtttagcgtc tcactctatt tgtttatcta
    26701 ttcatgcata ctagtatcgg tagtctgaac gttgcctttc ttgacatggg cagcttgtgt
    26761 tcctcgattc ccttcgatga tctcaaattc gaccacttga ttttcttcca aagttttgaa
    26821 tccctcttct tcaatcgcag taaaatgtac aaaaatttct tctgtgtcat cataactgat
    26881 aaagccgtat ccttttttgt tgtcaaacca ttttacaatt cctgtggtca aagcactcac
    26941 gacctttcta aagtctgatc atagccttca ttataaagag tatttataag tacgtcaaat
    27001 aaattaaaaa cctgtaattt tatgtttttt ttctgaataa aaaaaggtat aattggcata
    27061 tcttaacaaa gaagatattt tgttgaataa agttcgtgaa aaggggtaat caaatgaaga
    27121 atgatctgca aatttcacaa gaaacagagc tagaaccgat tgttgaagtg gctagtaaat
    27181 tagatcttaa ggaagacgat ttggaactgt atgggaaata taaggcgaaa atcaattttt
    27241 ctggaatcaa tcgatcaaag gataacgctg aggggcattt gatcttagtt acttccatca
    27301 atccaacacc agctggcgaa ggaaagtcaa cgatcactgt agggttagga gacgcgctca
    27361 ataaaatcaa taaaaaatct gttattgcat tgcgagaacc ctcacttggt cctgtgatgg
    27421 gaatcaaagg tggtgctgca ggcggaggat atgcacaggt attgcctatg gaagatatca
    27481 acttgcattt tactggtgac atgcatgcta tcacaacagc taataatgca ttatctgctc
    27541 ttttagataa tcatattcat caaggaaatg aattgaacat cgatgcccgc cgagtgattt
    27601 ggaaacgggt agtcgatctc aatgatcgtg aactgcgtaa agtaattgtt ggcttgggtg
    27661 ggccaatcca aggtgttcca agagaagatg ggtttgatat cacagttgcc agcgaaatca
    27721 tggctatctt gtgtcttgcg gcagaccttg aagatttaaa ggctcgtctt gcaaggatcg
    27781 ttgtcggcta tacatttgac cgtcagcctg tgactgctgg agacttgaaa gcagaaggcg
    27841 ctctggcatt actgctgaaa gatgcaatca agccgaatct tgtacaaaca atttacggaa
    27901 caccagcttt tgtccacggg ggaccgtttg ccaatatcgc tcatggctgc aatagtattt
    27961 tagctaccaa gacagcattg agattagcag attacgttgt cacagaggca ggatttggtg
    28021 cagatcttgg cggagagaaa tttttagata tcaaagtacc taacttgaaa aaagcgccgg
    28081 atgctgtggt gattgtcgcc actgtccgtg cattgaaaat gcatggcgga atgaaaaaag
    28141 atgaacttaa aaatgagaat cttgatgctt tgaaagtagg ttttgcaaat ttaaagcgcc
    28201 atatacgtaa tatggaacaa tatcagctac ctgtgatcgt cgcaatcaat gaattcgtaa
    28261 cagatacaga caatgaactc acactgttgg agcacttgtg tgaaaatcag gggattcttg
    28321 ccaagagagc gagtgtatgg gcagatggag cagaaggcgg cgttgattta gcaaaagctg
    28381 tagtacgatt gattgataga aaagaagcta attacaagcc attataccgc ctagaagaaa
    28441 cgatacaaga aaagacagaa acaatcgtca agaaaattta cggaggaaat ggtgttgttt
    28501 tcagcaaaaa agcgcaaaaa caaatagaag agtttacgaa gaatggatgg gacaaactgc
    28561 cgatttgtat ggcaaaaaca cagtattcat tttctgatga tccttcactg ctaggtgcac
    28621 cggaagattt caccatcacg atccgcgaaa tcatcccaaa attaggggca ggatttttag
    28681 tagccttgac tggagatgtt atgacgatgc ctggtttgcc taaaaaacct gctgcattga
    28741 atatggacgt gacaaatgac ggagaagtgt tgggattgtt ttaagcttga ttataatcta
    28801 ccatcgaagg agctgtattt tgactacagc ttcttttttt agacgatagg gaattaattt
    28861 tgtttacact aggatcattc tttttcttta ttgtattttg agctatacta aagcataata
    28921 ggagggtatg atgaaaacga catttataag aaaaaccggt ttttacggta tgggtagctc
    28981 gattcagcta agaatcaaca atgaaaaacg cagtgttctc agtcatgaac aaaagataac
    29041 gcttgatctg gaagtgccct ttacgatgca ggtcaccttt tattggctaa aaagccccgt
    29101 ttatacagtg aaagaagcaa agcctgctta tgtgatcaca atgaattact tgattcttca
    29161 aatctatccg tttttatttc tagcaactgg gttgggggcc gttttcctac aaaatttatt
    29221 tttcagtttt tttgcagtta ttgtgttgat tgcgttcttt ttctatataa aaaaccgggc
    29281 atatttgatc aaggaggaaa attatggcaa actctgatct ttttttaatc agtttcaatc
    29341 gaatcgaaaa atggatgaga gacgagatgg gaaatgcacg aaatatggga tttaccgaac
    29401 ttgtcagacg cctagctcaa agaaaacaat taatgatcag aaaatacgaa gatgatcttt
    29461 tacagcttgc tcagttaagg aatgcgatcg tccatgatcg gatcgcagtc gattttgtca
    29521 ttgcagagcc aaatgattgg acagtcaagc gaatccagct gatcgaacaa gaactgattc
    29581 agcctgaaac cgtgctgcct cgatttgcaa aacgtgtaac aggttttgaa caagatcttc
    29641 cgttacttga actgttaaag atagtagcag aaaaaaggta ttcacaattt ccgttataca
    29701 ataaaggaaa atttgtagca ttgattactt tacgtaatat cgggttttgg ctagcaaaag
    29761 aaagccaaaa aggtccggtt gatttaacaa ataaacgagc gttggatttg atcatccaaa
    29821 acggaaagta cacaaattat cattttgttc cagctaaaac acatatttac gaggttgaag
    29881 cgatgtttcg tgagcaaggg actttggaag ccgtacttat cactaaagat gggaatcccg
    29941 atggtaatct gctcgggatc gtacgacctc gtgatattta taaacaaata gaaaaggatt
    30001 gattttttgt taattattta ttggatattc agcgctttgc tagtaggagt ggatcagttg
    30061 gtcaaatggt ggatcactga taatctagcg cttggagaga cgaaaaatct tataccaggt
    30121 attttgtcac tgaatcatat tcgaaacact ggagccgcat ggagtatgct agaagggaag
    30181 atgtggttct ttactatagt gacactcatt gcagtcgttg tgattctcac attgatgatt
    30241 aaaaatcgtg aaaaaggtaa tcgctggttc atgattggat tgagtttgat tctagctggt
    30301 gcgattggca attttatcga ccgtattcgt cttggctatg tagtagatat gttccagaca
    30361 gattttatga gctttccgat ttttaacgta gcagatgtga cacttgtatg tggagtgatt
    30421 tgtatgttaa tttatattat tttagatgaa aaggatcaaa gaaaaaaatg acagaactta
    30481 aaacgacgat tcaccatgaa tctggtagga tcgataaagt attgactaat ttattcaccg
    30541 attacagtcg ttcgcaaatt cagctgtggc tgaaaaatgg agcggtttct gtcaatggac
    30601 agccagtcaa agcaaattac aaagtaaaac aaggagacga gattactgtc ttgatccccg
    30661 aaccagaaac attagatgtg gtcccagaaa atatcccgtt gacgatcgtt tacgaagatg
    30721 aagatgtagc ggtgataaac aaacctcaag gaatggtcgt tcatccttca gcaggtcata
    30781 cgcatggcac aatggtcaat gcgctgatgt accatatcaa ggatctttct acgatcaacg
    30841 gcgttattcg tccaggtatt gttcaccgaa tcgacaaaga tacttccgga ctgctaatgg
    30901 ttgccaaaaa tgaccaggca cacgaatcat tagctcagca attaaaagat aagacctctt
    30961 tgcgcaaata tgttgcttta gtccatggag agattcctca cgaaaaagga accatcaatg
    31021 cgccaatcgg ccgttcgaag gaaaaccgga aaatgcaggc gataagagaa gatgggaagt
    31081 cggctgtcac acattttact gtattggaac gtttcgaagg atttactctg gttgaattaa
    31141 aactagaaac cggaagaaca caccagatcc gtgtgcatat gaagtatatc ggttatccgt
    31201 tagctggaga tccagtatac ggaccgaaga aaactttgaa aggcaatggt cagtttctcc
    31261 atgcgcaaat gttaggtttt acgcacccga ctacaaacga aaagatgatc tttgaagcac
    31321 ctcttccaga agtttttgaa aaaactttag aaaaattaag aaaaaacatt gcattttaat
    31381 ttagataacg gtatagtaat taacaacgaa agcataaaac ttaatagaat tcctttaagt
    31441 ttagtcccgt gaggctaaga agggtgggag caaggatacg tatggtgcgc cataagtaga
    31501 ttgctcattg acatagtata gctatcagtg tatcagtata cgctcagtct aaacattcct
    31561 cctgtcctct tagggcagga gtttttttat gcaaaaaagt agggggaatg aaaatgcaag
    31621 caaaagaagt agtagatcag gtaaccatga agcgagcatt gactcgaatt acttatgaga
    31681 ttatcgagag aaaccatagt atccaagata tcgtattggt aggtatcaaa acacgaggaa
    31741 tctacattgc atcaagaatt gccgaacgcc tgaagcagct agaagatatc gatattcctg
    31801 taggagaact ggatatcact ttataccgag atgacaaaaa agaaaatcca gaagaaccgg
    31861 aacttcattc ttctgacatc ccagtttcct tagaaggaaa agaagttatt ttgattgatg
    31921 atgttttgta tacaggacga acgattcgag cagcaatgga cgcagtaatg gattttggca
    31981 gaccaagaaa aatttcatta gcagtcctgg tcgatcgtgg gcatcgagaa ttgccaatcc
    32041 gtgcagatta tgttgggaag aacattccaa catctcgggc ggaagagatt ttagtagaga
    32101 tgcaagagct tgatggacaa gatcgcatca tgattttgaa agaggaagat tagaggagag
    32161 agaaacatgt cggagaaaaa aggatttcat aatgatgacg tggtgttaga tatccacgat
    32221 cgcccaaccc caggacattg ggtaggatta agtttacaac acttgttcac gatgttcgga
    32281 gcaaccgtgt tagtccccat cttagtaggt atcgatccag gtattgcgct agtcagttca
    32341 ggactaggta cattggtgta cttatttact actaaaggaa agatacctgc gtacttagga
    32401 agcagcttcg ctttcattgc agcgatgcag cttttgatga aaacagatgg ttatccagcg
    32461 attgcacaag gagcagtcac aactggatta gtctatttga ttgttgcgct gatcatcaaa
    32521 aagatcggtt cagcatggct ggataaaatc ttgcctccaa tcgtcgtcgg tccagtcatc
    32581 atggttatcg ggttgggact tgcggcaaat gcagcaacaa atgctatgta taatcagggc
    32641 acgtatgatt tcaagtatat tgctgtagcg ctgatcacat taggattaac aattttcttc
    32701 aacatgtggc taagaggttt tctcggattg attccgattt tactaggtat tattagcgga
    32761 tacatcgtag ccttgctgtt tggaatcgtg gatactaagc caatcgtaga cgctgcctgg
    32821 tttgctgttc ctaactttga gattcccttt gtacaatatg aaccaaagct atatcttgga
    32881 gcaatcacaa caatggcacc catcgctttt gtgacgatga cagagcatat cggccatctt
    32941 atggtattga ataaactaac taaacgtaat ttctttgaag aaccgggtct tcataagacg
    33001 ttgacaggtg atggattagc acaaatcgtg gcagcctttg tcggaggtcc tcctgtaaca
    33061 agttacggtg aaaacattgg tgttctggct atcacacgtg tccatagcgt gtttgtgatc
    33121 ggtggagctg cagtatttgc agtcatttta ggattcgtag gaaaattaag cgctttgatt
    33181 ttaagtatcc ctggaccagt catctcagga atcagtttta tcttatttgg agtaattgcc
    33241 gcaagtggaa tgaagatttt aatcgagaac aaaatcgatt ttgataaaaa gaaaaatctg
    33301 ttgattgctt cagttatctt agtagtagga attggcggac tcgtctttga agtaggcaca
    33361 tttacattgt cggcaatggc attagcaact gtacttggca tcattttaaa tcttatctta
    33421 ccagaaactt cacgaagcga agaaaactaa ggaggaaaca ttatgatcat caaatcagaa
    33481 cgtatcagct taaagcattt attgaccgtt gaggcactta ctgatcaaga agttatgggc
    33541 ttgatccgca gagggcagga attcaagcaa ggtgctaatt ggactccgca aaagcgccag
    33601 tactttgcta caaacctctt tttcgaaaat agtactcgca cacataaaag cttcgatgta
    33661 gcggagaaaa aactagggct agaagtgatt gaatttgaag caagcacaag ctctgtccaa
    33721 aaaggggaaa cactatacga tacagtactg acgatgtcag cattaggcgt agacgtagcg
    33781 gttatccgtc atggtgatga gaactattat gacgaactga tacaaagtaa aacaatccag
    33841 tgttcgatta tcaatggagg agacggaagc ggacagcatc caacccaatg cttgcttgat
    33901 ttgatgacca tttatgaaga atttggtcat tttgaaggtc tgaaagttgc catcgtaggt
    33961 gatatcactc actctcgcgt agctaaatcc aatatgcaga tgttaaaacg tctaggcgca
    34021 caagtattct tctcaggtcc tagagaatgg tacgacgatg aattcgaagt gtacggacat
    34081 tatatgccat tggatgaact actagatcaa gttgatgtca tgatgatgct tcgggtgcaa
    34141 cacgaacgcc atgatggaaa agaaagtttc tcaaaagaag gctaccatca ggaacatggg
    34201 ttaacagttg agcgtgcgaa gaagttgcaa aaacatgcaa tcattatgca cccagcacca
    34261 gttaaccgtg atgtagaatt agctgattca ttagtggaag gattacagtc gcggatcgtt
    34321 cagcaaatga gcaatggagt attcgtcaga atggcaatct tagaagcagt gctaaatgga
    34381 aaagcgtaat tataggagga tgaaaaaatg aaaacactca ttaaaaacgg tcagatcaat
    34441 acacgaaaaa atatgacgac accagcagaa atttggatag aagatgggcg aatcaaagca
    34501 atcggtacag gattctcaga agccgaattt gatgaagtgt ttgatgcaaa gggacaattg
    34561 atcacacctg gtttagtgga cgtccatgtt catttgagag agccaggatt tacatacaag
    34621 gagacgatcg aagctgggac tcgctcagct gctcgtggcg gtttcactac tgtatgcgcg
    34681 atgccgaatt taaatcctgt acctgatacg gcagaaaagc tgagacaagt atatgacatc
    34741 attagaaaag atgcagttgt gaaggtattg cagtatgcgc caatcacaga aaatctgcga
    34801 agtgagaaat tagttgatca agaagcactg attgaagaag gtgcttttgc tttcacaaat
    34861 gacggtgtag gtgtccaaac agctgggacg atgtatctgg ctatgaaaga agctgcaaaa
    34921 aacaataaag ccctagtagc ccatacagaa gatgaatctt tattatttgg cggcgttatg
    34981 cacgctggca agaaggcaga agaacttggt cttcccggga tcttaagcgt aaccgagtct
    35041 tctcaaattg ctagagatct gttgctagct gaagcaactg gtgttcatta ccatgtttgc
    35101 catgtttcta ctaaagaaag tgtccgtgta atcagagacg caaaaaaagc aggaatccac
    35161 gtaacagcag aagtttcacc ccatcatctt attttgatcg acgaagatat tcccgaagat
    35221 ttcggctttt ggaaaatgaa cccgccgcta agaggacggg aggacagaga agctttgatc
    35281 gaaggcttgc ttgacggtac gatcgattgc atcgctacag accatgcccc ccatggatta
    35341 gaagaaaaaa gccaaagttt catgaaatca ccatttggta tcgtaggaag cgaaacagcg
    35401 tttcaactga tctatactca ttttgtagaa actggtcgat tcacgttgga acaagtgatc
    35461 aattggttgg cagttaaacc ggctgagatt tttggtttga atgcaggaac gctaacggtt
    35521 ggtgcccccg ctgatgtagc agtttttgat attacacaaa cttgcacgat cgataaagaa
    35581 gactttttat caaaagggga gaatacacct tttatcggct ggaaagtaaa aggcgaaaca
    35641 caaatgactt ttgtcaacgg aaaacttgtg tggcagaagg gagaataaaa gtatggaacg
    35701 tttgcttata ttagaagatg gaacagtttt cgaaggaaag gcttttggtg cagatgctag
    35761 tgtcttcgga gaaatcgttt ttacaacaag tatgactggt tatcaagaaa ctatcactga
    35821 tcaaagtttt aacggtcaaa tcattacatt tacctatcct atggtgggta attatggggt
    35881 aaatagagat gattatgaat cgatttctcc aacatgcaaa ggagtagtag taaaagaaca
    35941 tgcacgtgta gcttcaaact ggcgaaacca aatgacgcta gattcatttt tgaaacaaaa
    36001 aggaattcct ggaatatcag gtatcgatac gcgcgccctt actcgcaaaa tccgtgaagt
    36061 cggaacgatg aaagctagta tcgttgatgc tggagattcc ttcgaacatg catttgatca
    36121 gctgaaagca accgttctag caaccaatca agtgcagcaa gtctcaacta acaaacctta
    36181 tccaagtcca ggaacaggtc ggaaagtcat tgtcattgac ttcggcttga aacatagtat
    36241 tttacgagaa ttgagtaaaa gaaactgtaa cctcacagta cttccctata acacagatgc
    36301 cgagacgatt ttagcgctat caccagacgg agtcatgctg acgaatggac ctggtgatcc
    36361 aaaagatgtg ccagaagcaa tcgaaatgat caaaaaaatc caaggaaaag taccgatttt
    36421 tggtatttgt ctcggtcacc agttatttgc tttagccaac ggtgcagata cctataaaat
    36481 gaaattcgga catcgaggat taaatcaccc ggtacgagaa gtagcgaccg gaagaattga
    36541 ttttacttct caaaatcatg gctatgcagt cgatgaagcc acgattgatc cagaaaaact
    36601 catcgtaact catgtagaag taaatgacgg aacagtagaa ggagtcaaac acagagatta
    36661 tcccgctttc agtgtgcagt ttcatccaga tgctgcccct ggtccgcatg atgcactaca
    36721 tttatttgat gaatttatgg aaatgatgga cgcaggaaag gagcagtaaa cagatgccga
    36781 aacgtacaga tatcaagaaa attatggtga ttggatctgg tccgatcatc attggtcaag
    36841 ccgcagaatt tgactacgca ggaacacaag cctgccttgc cttaaaagaa gaaggatatg
    36901 aagtggtttt agtaaattct aatcctgcga cgatcatgac ggataaagaa gtggcagatc
    36961 aagtatatat cgaaccgatc acacttgaat ttgtatcaag gattcttcga aaagagcgtc
    37021 cagatgctct ccttccaact ttaggcggac agacaggcct caatatggca atggagctgg
    37081 cagcatcagg tattttagaa gaattgaaca tagaacttct aggaacaaag ctttctgcga
    37141 tcgatcaagc agaagatcgt gatctattca aacagttgat gaaagaattg aatcagccaa
    37201 tccccgagtc tgaaatcgtc aatacagtag aagaagctgt agattttgca aataaaatcg
    37261 gctatcctgt catcgttcgt ccagccttta ctttaggcgg aactggtgga ggtatgtgtg
    37321 aaaatgaaga agaattacgt atcgtatcag aaaatggatt aagtctttcc cccgctaccc
    37381 aatgtttgat tgaacgaagt attgcaggtt ttaaagaaat tgaatatgaa gtgatgagag
    37441 attcggctga taatgcaatc gttgtctgca atatggaaaa ttttgacccg gtgggtatcc
    37501 atacaggtga ttctatcgta tttgccccta gtcaaacatt gtctgaccat gaatatcaaa
    37561 tgcttcgtga tgcttctctt tcgattattc gagcacttaa gatcgaggga ggctgtaatg
    37621 tccaactcgc tcttgatcca catagtttta attattatgt gattgaagtc aatccgcgtg
    37681 tttctcgttc atctgcgtta gcaagtaagg cgactggcta tccgatcgct aagctagctg
    37741 ctaaaatagc gcttggatta acattagacg aaatgaaaaa tcctgttaca ggaacgactt
    37801 ttgcagaatt tgaacctgca ttagattatg ttgtagctaa aattcctcgc tggccgtttg
    37861 ataagtttga aaacggagaa cggcttttag gcacgcagat gaaagcaact ggtgaagtga
    37921 tggctatcgg tcggaatatc gaagagtctt tattgaaagc tgtgcgttct ttagaaatcg
    37981 gtacacacca tatcgaactt cctgagttgg cagacatcac ggacgatgac ctaatccaaa
    38041 aagtggttcg agcacaagat gatcgcctgt tttatgtagc tgaagctatt agaagagggt
    38101 acaccataga agaattagcg gatttgacaa aaatcgattt attcttttta gataaactgt
    38161 tgcatatcgt agaaatcgaa cagcagttga aacagcagcc gttggatgac aagatattga
    38221 aaacagcgaa acaaaacggt tttacagaca aaaaagtggc ggagctgtgg gaaataaacg
    38281 aagaagccgt tcgccagatg agaaaagaaa atgatttgat tcctgtatat aagatggttg
    38341 acacttgtgc agcggaattc gaatcaaaaa caccttattt ttatagttcc tatgaatatg
    38401 aaaatgaaag ttcacgatca gaaaagccct caattctagt actgggatct ggtcctatcc
    38461 gtatcggaca aggggttgaa tttgactatg cgactgtgca ttctgtcaaa gcaatccagc
    38521 aggcaggata cgaagcaatc atcatgaaca gcaatcctga aacagtttcg actgactttt
    38581 ctatttcgga taaattatat tttgagccat tgaccttcga agatgtgatg aatgtgatcg
    38641 agctagaaca gccgatagga gtaatcgttc aatttggtgg tcagacagca atcaacttag
    38701 ccgaaccttt gactgctgct ggtgtaaaaa tcttagggac aacgatcgaa gacttggatc
    38761 gtgcggaaaa tcgtgattta ttcgagcaag cgctgaaaga gttggatatt cctcaacctt
    38821 taggggatac tgctacaagt aaagaagaag ccgtggttat cgcttcgaga attggttatc
    38881 cagttcttgt tcgtccaagc tatgtattag gcggacgtgc gatggaaatc gttgaaaacc
    38941 agcgagattt agaagattac atggaacatg ctgtgaaagc atctccagaa catccagtac
    39001 tagtggaccg ttacttgctt ggtggtgaat gcgaagtgga tgcgatttgt gacggtgaaa
    39061 cagtactcat cccagggatc atggagcata ttgagcgtgc tggtgtccat tcaggggatt
    39121 caatggctgt gtatccacct caaacattat ctgaatcgat caagaaaacg atcgaagatt
    39181 acacgatccg cttagcatta ggtttgaatt gtgtcgggat gatgaatatc cagtttgtca
    39241 tccatgaaaa tgaggtttat gtgatcgaag tcaatcctcg agcaagtcgg actgttccat
    39301 tcttaagtaa ggtgacagga attccaatgg cacaaattgc gacgaaagct attttgggag
    39361 agaaactaaa gacacttggt tatgaaaacg gactttatcc agaaacaaaa gaagttcatg
    39421 ttaaagcacc tgtcttttct tttacgaaat tgcagaaagt tgatacttac ttagggcctg
    39481 aaatgaaatc gactggagaa gtaatgggat ctgacagaca attagacaaa gctttgtaca
    39541 aggcattcga agcttctgga ttacacttgc ctgaatatgg agccgtatta tttacgattg
    39601 cggacgaaac aaaagaagaa gcactagaat tagctaaacg ttttacagaa attggctata
    39661 gtctgatagg aacgaaacat acagctgctt atttcgaaaa aaatggtctg gtcgttgaaa
    39721 cagtagcgaa aatttctgag gaagcagcag aaaaaaatgt agtcgactta ataagagatg
    39781 gtaaaacaca agtggttgtc aatacgattg ataaagatcg gggaaatgct tctaaagacg
    39841 gtttcatcat ccgaagagaa gcagttgaac atggtattcc attgtttact tctttagaca
    39901 cagcagatgc gatcgttcga gtgatggaat cccgtgcttt ttcaactaac cctatttaac
    39961 ggcgagcaca tgttaagaca aaaagacgaa aaaccgttgc aggaggggac agatgaaaca
    40021 agaaatgatg gaaatcgttc agcaaaaaca gctggcacca aagatttttg aaatgacatt
    40081 gaaaggtgat ctagtaaaag aaatgcagca accaggacag ttcttgcata ttctcgtgcc
    40141 gcgtgcagac ttgctattac gccgaccgat tagcttgaat catatcgacc acgaaacaaa
    40201 tacttgccgt atcatttatc gggtggaagg tgaggggact agagtcttct ctactctttc
    40261 tgccggagac catttagatg tcatgggtcc tttaggcaat ggttttgatt tgtccaatct
    40321 tcatacagga gacgaagtat ttataatcgg tggaggaatc ggtgtccctc ctctatatga
    40381 gttatcaaaa cagttagtcg aaaaaggaat taagccaacg cattttttag gttttgccac
    40441 acatgaagtc gtttattatg aaaaagaatt taatcgtcta ggagaaacaa gaatcgctac
    40501 tgacgatgga acatacggta tgcacggaaa tgtcggtaat ttattgttag gaacgaagac
    40561 ggaacctaca gctgtttttg catgtggttc aaacgggtta ttgaagacag tggaacagct
    40621 gttttcttcg catcaaaatg tccagttatc attagaatcc cgcatggcat gtgggatagg
    40681 ggcttgctat gcttgcgttt gtcatacgcc ggaagatgaa acaggaacaa aaagtgtgaa
    40741 agtttgtgac gaaggaccga tttttaaaat aggagaggta attatatgaa tgagaatcct
    40801 ttagcggtca aacttcctgg cctagaatta aaaaatccta tcatgccggc aagcggctgt
    40861 ttcggttttg gcaaagaata cggaaaatac tatgatttga atcagcttgg atcgatcatg
    40921 gtcaaggcaa cgactccaaa tgcccgcttt ggcaatccca cgccaagagt ggcggaaaca
    40981 ccatctggca tgttgaatgc aattggtttg caaaatcctg gtctagacgt tgttatgcat
    41041 aaacttcttc cagaattaga ggagtacaat gagttaccca tcatcgcaaa tgttgctggt
    41101 gcttgtgaag aagactatgt agaagtttgt agcaaaattg gcgaagcacc aaatgtgaaa
    41161 gcaatcgagc tgaacatttc ttgcccaaat gtcaaacatg gcggaattgc ttttggaaca
    41221 gattctgatg ttgcttttca attaacacaa gcagtaaaga aagtttcttc tgttcctgtc
    41281 tatgtcaaat tgtccccaaa tgtaacagac atcgttccaa tcgctcaggc aatcgaagct
    41341 ggaggggcag atgggtttac gatgatcaat actcttcttg gtatgcggat tgaccttaag
    41401 acaagaaaac cgattttagc caatcaaaca ggtggactct ccggaccagc aatcaagcct
    41461 gtcgctattc gtttgattca ccaagtagca agtatatcca gccttccaat cattgggatg
    41521 ggcggcgtgc agacagtgga tgatgttctt gagatgttta tggctggagc aagtgctgta
    41581 gctataggga ccgcgaattt caccgatccg tatatctgtc ctaaactaat taaagagctc
    41641 cctgtgcgaa tgagcgaact aggcatcgaa tcgctggaaa gattaatcaa agaagtgaga
    41701 gaggaacgtg aacgatgaat caactagacc aacgaccaat tattgcatta gatttttcaa
    41761 ctaggcaaga ggtagaagac tttcttcgat ttttcccgaa agaagaaaaa ttgtttgtga
    41821 aaatcggaat ggaattgttc taccaagaag gacctgaaat cgtacgttat ctaaaagagg
    41881 ctggtcataa tgtattttta gatctgaaac ttcacgatat tccgaatacg gtcgaaaaag
    41941 caatgcgggg attggcaaag cttggtgttg atctcacctg tgttcatgcc gctggcggta
    42001 ttcgtatgat ggaggcggca atgcgaggat tagaagaggg gactccagaa ggtggaaaac
    42061 gtccgctact tctagctatc actcagctta cttctacaag tgaagaagaa atgcatgccg
    42121 atcaactaat cgaagtttct ttagaaaaaa gtgtgatcca ttatgctagc tgtgccaaaa
    42181 aagcaggatt agatggtgtg gtttcttctg cttgggaagt agaagcgatc aaagaaatgg
    42241 caggagacga atttgtttgc ttaactccgg gtataagacc agaaggaaca gtcgcaggag
    42301 atcagacaag agtagtcaca ccttctcagg caaaaaagat cggctcaact tttatcgtta
    42361 taggtcgacc aatcactcag tcaactaatc cttataaagc atatcaaacg attcaaacag
    42421 aatggagcta acctaaaatg aatgttgaac aaagtatcgc aaaagactta ttagaaattg
    42481 aagcagtatt tttaaatcca agtaatcctt ttacatgggc aagtggaatc aaaagcccaa
    42541 tttactgtga taaccgaatc acgatgagct atccaaaagt ccgaaaagag atcgcaaaag
    42601 gattagcaag caaaatcaaa gaagcttacc ctgaagtaca agtgattgct ggaactgcaa
    42661 cagcaggaat ccctcatgcc gcatgggtag cggaaatact ggatttgcca atggtctata
    42721 ttcgaagtaa ggcgaaagat catgggaaag gtaaccaaat cgaaggacgt atatttgaag
    42781 gtcagaagat ggttgtgatc gaggatttga tttctacagg cggaagtgtc ttggaagcag
    42841 ctgaagcagc aaaaagagaa ggtgcggata tccttggtgt tgcggctatc tttacgtatg
    42901 agttgccaaa aggaaaagca aatttcgaaa aagccgaaat ccctcttatg acgttgacca
    42961 attattctgt attgattgaa gcagctttag aagatcgtta tatcgatgag caagaattaa
    43021 ctctgctaaa agaatggaaa aaagatcctg aaaattggca gatttaagcg cgcttgatga
    43081 agatagagaa atccgatttg tgtctccaga atcatcaatt ggaatagcaa aaatccaaaa
    43141 actgattgcg agaagcatct tttcatcagg tatgaaaaga aacttctatc gtgatcagtt
    43201 tttgattttt ttaaaattca tttttctaag actggttagc agaaagaaga gaataatctc
    43261 ttctcttttt tctattatat atagattttc tctatttttt ggtaagggag ctgtttcaaa
    43321 aatggtaaag agcggaaatg ttgcgaatat cagttattta atgatatgat attcggaaaa
    43381 agaaatctca aagggaggta acttttatgg ggttgaatga atttatttta ggattgaaca
    43441 atttagagac gatcacaagg gcacctggat tttttaaata tacagagcat acagttgcag
    43501 cacattcgta tcgtgtagca tctattgcgc aagttctagg agacattgaa gaagcgaatg
    43561 gacaaccgat cgattggaaa ctgctctatg aaaaatcatt gaatcatgac tacacagagc
    43621 gttttattgg tgatatcaaa acgccggtga aatacgcaag caaagagtta agagggatgc
    43681 tgcaaacagt tgaagaaaag atgacggacg aattcatcaa ccaagagatt ccagctgagt
    43741 tccaagaagt ttatcgccgt cggttgttcg aaggaaaaga tgaaaccgta gaaggagaac
    43801 tattagccat ttcagataag gttgaccttc tttatgaatc ttttgatgaa atcatcaaaa
    43861 ataacccgga aaaagtttat aaggagatgt tcattgaggc agtgactacg attaaaagct
    43921 ttgaacatcg gccttctgtt cagtatttct ttaaagagat atttccggaa ctattgaacc
    43981 aggaatttta tggaaaagag gcgtttttag aaaccgtctc aacgattgta aagaaagaag
    44041 aaaaataaaa atgatatgag atgcaggggg aagtatagtt gaaaaagaat atggatgtcg
    44101 aatggggcta ttctggagac aaaggtccgg aaaattgggc gagtttatgt gattggtttg
    44161 cgagaggtgc ggaatttcca ttgcagtcgc ctattcattt gaccaagggg gaagaaacca
    44221 aaatagaagg agacacgctt tcctttcatt atcaaaagca acggtttact gacaaggaat
    44281 tcaaaaacac gattcattta gtaccttttg accaactcag ttatgtagag ttcaagaaag
    44341 aacgctatta tttgacagat attcatttcc acttgccttc tgaacatatc atcaacggaa
    44401 aacaggaaga tattgaattt cattttgttc atatgaacag caaaaaagag aatctagttg
    44461 tgggtgtcat gtaccagcta atggtacagg aaggttggct gtataatcaa gaaaatgggg
    44521 aaagttggaa cttagaaaaa catgagcatt gggtaaatcc ggatgtgttc tttccggagc
    44581 aaaaaagcca tttccattat attggctcat tgacaactcc gcctaccagc gggccaatcc
    44641 aatggtttgt aatggatcat gtaggaaaac tgaaccaaga gtttcttctc cccttcgatg
    44701 gtcagtatgc tcgaccgaac aaccgaccaa tccagccctt gaatggaaga gaaatttatt
    44761 atttcgagga gtttgagcaa tgaatattca gcagatgaaa tacgtggtcg ctgtagcaaa
    44821 caatggtagt ttcagagaag cggcaaagaa aatgtttgtc tcccagccaa gtctttcagc
    44881 tggtatcaaa gaattagaaa ccgagttagg gatcacgctg tttactcgaa cgaatcgagg
    44941 ggcatattta acagaagaag ggcaagactt cttaaatcgt gccgagaaaa tattggtcca
    45001 gcttgataca ttagaaactc actatctaac gagtgaaaag agagacaatt tttcgatcgc
    45061 tgcccagcat tatgatttct taggtccttt gaccgcacag ctactcgagg aattcaaaga
    45121 cgagatcaaa caatttcgga tcatggagac gacaacagcg agagtgatcg aagaagtcaa
    45181 agagcagcac agtgagatcg gcattattta tatgaatgag cataatcaag ctggtatcag
    45241 aagatattta caacaaggag agctaagtgg ccgcagcatt ggaaaattca aaacgcatat
    45301 ttttttaagg caaggacatc cattagctga acacgcagag atcaccaaag aagaattgtt
    45361 atcttatcct caagttcgtt ttacacagga tggaaataat ttcccgtatt tttatgaaga
    45421 ccttatcgag atccctgatc aagaaacagt gatttatacg agtgatcgag gtactttgat
    45481 gaatatcgtt cttgaaactg atgcttacgc ttcaggatct ggtattgtga ttggagggat
    45541 tcgcgagcac ttgcggttaa tccctttggc agaaggcgaa ctaaatgaat tttatatcat
    45601 ttattcggcg aaacgtccgc tcagtgaaat cgctcaaaga tttatcaaag agcttactcg
    45661 gttactggac cagcagagct aagaaaagca gatgagaacg ctgtttattg aacaccgttg
    45721 atcaaaaaag aggggttgtg acagaatttt gtcacaactc ctctttttgt agcaaacaat
    45781 ttataactaa ttgctgttgt gggaaagtac gcaaatatga ttctagaaac actcattgat
    45841 tacctgtttt gtttcatagc tgtaattttt tcttcttctg gtgtcacgat aatcgtcttt
    45901 tggttttcat aaataacata accaggcttg gcgccatttg gttttcggat atgctttacc
    45961 tgtacaagat caaccggcac ttgggcagag tatcggtatt ttgaaaaata ggcagctaac
    46021 tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac ttttaatgat gacgtgcgaa
    46081 ccgggtatat tttttgcatg aagccaatag tcggtttttt tagctgtctt cattgtcagc
    46141 tggtcatttt gaagattgtt tttaccaaca agaatgagcg ttccgtcaga agaaaagtat
    46201 tgatcaggct gagatggttt cttacgtttt tgttttttta agttcttctt tttcaggtat
    46261 ccttctgaag tcaattcttc tttaattgct tcgatatcca ttgggccggc aatttccagt
    46321 tgagataaga cagattcaag atattggatt tcatctttgg cttcttggat ttgttcgcca
    46381 atcagcttaa cagcgttctt cagcttttgg tatcgatgga aatatttctg tgcattttga
    46441 tttggtgtca atgccggatc tagtgctatt ttgattggcc gatcttcttc ataataatta
    46501 ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca gaaaagtagt aagtagttcg
    46561 ccatttctac ggtaattctc agcgttttcg gattctttga gtgtttgttc acgttttttc
    46621 aatttatttt tatttcgttt tagttcattt tcgatttttc gaatcagttc tccgccttgt
    46681 tgttttgccc gatctttctc tgctttttcg tgataatagc tatctaataa ttgactgagt
    46741 gttggataag ctgttacagc agatgcctgt tcggacaata cctgataagc aatcggtgtg
    46801 aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag gctgatggct gatggctgag
    46861 aaaaattcgt cccatacaac catcttttca ttaggacgtt ccagtaatct tttggtcagc
    46921 tcttgagcga tatcgaaacc gattccctga aactgacgtt ggattgcttt tggatctagt
    46981 tcagtttggg aaagccgctg aaagattttt tctttctcgg agctaaatgg gttcaagacc
    47041 tcttgttttg gcggagcaac atattctaca ccaggaagca aagaacgata agtgttttga
    47101 gagcttccaa tgtgtttgat cgcatccagt atttttccag tttctcgatt aactaagacg
    47161 atcgtactat gacgtcccat taattcaacg atcaatatga tattttgtag atcacccaac
    47221 tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat tttcgatttg ttcgatgtct
    47281 tctaaaatag cgccatccaa atattttcga agcatcatca cgaagttagg cggattatca
    47341 ggattctgat aatcgatttg ggtaatctgt acacgagcat aacttgggtg cgcagataat
    47401 aataatcgat ggtttttgcc gcgtgaacga atcaccagca cgatttcatt ttcataaggc
    47461 tgatggattt tcgagatccg gccagatagt aacgtttcac gcaattcgtt gaccatcgcg
    47521 tgggtaaata caccatcaaa ggacatggtt cattcctcat ttcttttcat aagtatattc
    47581 attataacga aaaaaagaag aaaaaccaat ttggccgaat agtaaagcaa aagtctagaa
    47641 aaatcttgga ctaattacat aaaaaacagt gtttctatcg ttgaaatcca aatttttagg
    47701 t
//
LOCUS       DAPBAB010000017        41910 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPBAB010000017 DAPBAB010000000
VERSION     DAPBAB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.017"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..314)
                     /locus_tag="RD444_001169"
     CDS             complement(<1..314)
                     /locus_tag="RD444_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548555.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            436..1020
                     /locus_tag="RD444_001170"
     CDS             436..1020
                     /locus_tag="RD444_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548556.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1031..1276
                     /locus_tag="RD444_001171"
     CDS             1031..1276
                     /locus_tag="RD444_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548557.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1282..2004
                     /locus_tag="RD444_001172"
     CDS             1282..2004
                     /locus_tag="RD444_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548558.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2135..2878
                     /locus_tag="RD444_001173"
     CDS             2135..2878
                     /locus_tag="RD444_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548559.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2884..3249
                     /locus_tag="RD444_001174"
     CDS             2884..3249
                     /locus_tag="RD444_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548560.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3265..3795
                     /locus_tag="RD444_001175"
     CDS             3265..3795
                     /locus_tag="RD444_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548561.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3957..4187
                     /locus_tag="RD444_001176"
     CDS             3957..4187
                     /locus_tag="RD444_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548562.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVRLPVSELKK"
     gene            complement(4391..4870)
                     /locus_tag="RD444_001177"
     CDS             complement(4391..4870)
                     /locus_tag="RD444_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548563.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            4990..6327
                     /locus_tag="RD444_001178"
     CDS             4990..6327
                     /locus_tag="RD444_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDT7548564.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6350..6877
                     /locus_tag="RD444_001179"
     CDS             6350..6877
                     /locus_tag="RD444_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548565.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            6864..7427
                     /locus_tag="RD444_001180"
     CDS             6864..7427
                     /locus_tag="RD444_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548566.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            7850..8587
                     /locus_tag="RD444_001181"
     CDS             7850..8587
                     /locus_tag="RD444_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548567.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8604..9020)
                     /locus_tag="RD444_001182"
     CDS             complement(8604..9020)
                     /locus_tag="RD444_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548568.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9412..10659
                     /locus_tag="RD444_001183"
     CDS             9412..10659
                     /locus_tag="RD444_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7548569.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            10929..13319
                     /locus_tag="RD444_001184"
     CDS             10929..13319
                     /locus_tag="RD444_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDT7548570.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKIKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ENAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPTGYTGAFKQEGITVK
                     DDGQTFEYTATNTLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQIFEYTVINHRVPETVRTGGIGTSAFLLVFLVAGLGMGLLYYREQRKVK"
     gene            13368..15203
                     /locus_tag="RD444_001185"
     CDS             13368..15203
                     /locus_tag="RD444_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7548571.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSFGLGEQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGKPVSDIDNKGKEIALPNGVQPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPVQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     DYEKYVVKDVLGEELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTDSGMDKLQKKFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNRVTYLKQDPTTKAITWVEDKSKATEFVSGTDGKFQ
                     VEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSK
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15272..16135
                     /locus_tag="RD444_001186"
     CDS             15272..16135
                     /locus_tag="RD444_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7548572.1"
                     /translation="MSMKKKNIKLIILRIGMLLCLGVGLYPVITQAYTYYQTQQTLNA
                     YKEKYDEASEKENEEIFKKYSEINRKRKETEQTLVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYNGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFINVAGKKLAYKVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     ENSHRLLVRGVRIPYKKNIVEKEKKKEDLKVKAQINWYPIIVVGVLSIIALIVIIKKI
                     VAKRRGMKNEE"
     gene            16125..17204
                     /locus_tag="RD444_001187"
     CDS             16125..17204
                     /locus_tag="RD444_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7548573.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIDK
                     KQTPSKEEVAKNVGQILSNNENQKVNETQESDNESFDMDEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYIYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRSDVIKNYGQNSTLTDIPDL
                     DMLTLYTCHPFPQNYDRLLVRSLRDDKAKYITPQVTVGNTAYADAIDSESVSAKPETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQTSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVLGALASLKVLLLLIKEFRKA"
     gene            17543..18343
                     /locus_tag="RD444_001188"
     CDS             17543..18343
                     /locus_tag="RD444_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548574.1"
                     /translation="MVVISKTALDSLAKVFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18431..19237
                     /locus_tag="RD444_001189"
     CDS             18431..19237
                     /locus_tag="RD444_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548575.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19469..20338
                     /locus_tag="RD444_001190"
                     /pseudo
     CDS             19469..20338
                     /locus_tag="RD444_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            20358..20705
                     /locus_tag="RD444_001191"
     CDS             20358..20705
                     /locus_tag="RD444_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548576.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            20718..21980
                     /locus_tag="RD444_001192"
     CDS             20718..21980
                     /locus_tag="RD444_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548577.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGNYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            22060..23436
                     /locus_tag="RD444_001193"
     CDS             22060..23436
                     /locus_tag="RD444_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDT7548578.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            23433..24062
                     /locus_tag="RD444_001194"
     CDS             23433..24062
                     /locus_tag="RD444_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548579.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            24081..24560
                     /locus_tag="RD444_001195"
     CDS             24081..24560
                     /locus_tag="RD444_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548580.1"
                     /translation="MLELLEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQVEIDKAVKLNRLSKFNVLGNAF"
     gene            25062..25115
                     /locus_tag="RD444_001196"
                     /pseudo
     CDS             25062..25115
                     /locus_tag="RD444_001196"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25119..25718
                     /locus_tag="RD444_001197"
     CDS             25119..25718
                     /locus_tag="RD444_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDT7548581.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGESIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            25734..26210
                     /locus_tag="RD444_001198"
     CDS             25734..26210
                     /locus_tag="RD444_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548582.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGMKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            26227..27129
                     /locus_tag="RD444_001199"
     CDS             26227..27129
                     /locus_tag="RD444_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548583.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            27202..27393
                     /locus_tag="RD444_001200"
     CDS             27202..27393
                     /locus_tag="RD444_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548584.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            27557..28144
                     /locus_tag="RD444_001201"
     CDS             27557..28144
                     /locus_tag="RD444_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548585.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            28144..29043
                     /locus_tag="RD444_001202"
     CDS             28144..29043
                     /locus_tag="RD444_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDT7548586.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVSDATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            29043..29957
                     /locus_tag="RD444_001203"
     CDS             29043..29957
                     /locus_tag="RD444_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548587.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            30009..30224
                     /locus_tag="RD444_001204"
     CDS             30009..30224
                     /locus_tag="RD444_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548588.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NPVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            30415..30669
                     /locus_tag="RD444_001205"
     CDS             30415..30669
                     /locus_tag="RD444_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548589.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            30847..31890
                     /locus_tag="RD444_001206"
     CDS             30847..31890
                     /locus_tag="RD444_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7548590.1"
                     /translation="MKKILVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTIDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNIISRQGWKIEGTGWYAMPNS
                     APNPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPRAN
                     DNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAGTYE
                     KSFNVTGMAVAVR"
     gene            32066..32296
                     /locus_tag="RD444_001207"
     CDS             32066..32296
                     /locus_tag="RD444_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548591.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            32299..32556
                     /locus_tag="RD444_001208"
     CDS             32299..32556
                     /locus_tag="RD444_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548592.1"
                     /translation="MTNEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEEIMKREGFQTDNDSKKVE"
     gene            32671..33642
                     /locus_tag="RD444_001209"
     CDS             32671..33642
                     /locus_tag="RD444_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548593.1"
                     /translation="MKTKGFNVKENAIGGNKIFKKDIYVWFNGKKYMTTNKRWSELVQ
                     NTVKKVMPDGYHFNYIQCSQTTESIYFSILNENRLYYFRVSSHHRKKREDNVTTFLSG
                     KYNGFKELSEAIGEYLKSNRFVRISDKHILILLLYLNNSFKLFWRDDTFYYIENGMDI
                     YSGINGTLKHYIKALLRYGVLYQEDLSNQIKISNSGKSLLAKYKKLLSPIYKGIDMEA
                     VNSETLFNLVVDEKFANEFFKSKKIESLNNLFPENAIKAYVDNNKSRLYNWFIPKRLR
                     GKIKIGDQVKVINLNHTKIVTVTELYIAEKEEITKMKPVIKILDNKE"
     gene            33652..34443
                     /locus_tag="RD444_001210"
     CDS             33652..34443
                     /locus_tag="RD444_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548594.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTLL
                     EKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLGLAKKARDLRETQM
                     YLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMDKYRV"
     gene            34472..35590
                     /locus_tag="RD444_001211"
     CDS             34472..35590
                     /locus_tag="RD444_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548595.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKKTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETNTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            35623..35943
                     /locus_tag="RD444_001212"
     CDS             35623..35943
                     /locus_tag="RD444_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548596.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            36198..36899
                     /locus_tag="RD444_001213"
     CDS             36198..36899
                     /locus_tag="RD444_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDT7548597.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIGTVIDELISGKYDYAFDF
                     ENEKDYRSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYVDLSQYLGHDL"
     gene            complement(37041..38182)
                     /locus_tag="RD444_001214"
     CDS             complement(join(37041..37954,37954..38182))
                     /locus_tag="RD444_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7548598.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            38253..38480
                     /locus_tag="RD444_001215"
                     /pseudo
     CDS             38253..38480
                     /locus_tag="RD444_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            38500..40134
                     /locus_tag="RD444_001216"
     CDS             38500..40134
                     /locus_tag="RD444_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548599.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNNGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMSLTIKRIYDLLYVYKH"
     gene            40163..41332
                     /locus_tag="RD444_001217"
     CDS             40163..41332
                     /locus_tag="RD444_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548600.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRSKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQSNLIFDGRKLFFNLTISFEKRYNELEFKEGNELIVNFADLCSGECLVNNKSV
                     MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS"
BASE COUNT        15739 a         5747 c         8410 g        12014 t
ORIGIN      
        1 tcctcatatt catagctatc aattagctca gttgaactat aagctttttc tagtagttcc
       61 atttcccatt ttggagagtt tggatcactt tttcttagtc tagctatatc ttctaaaatt
      121 ttcttagaag tattatcctc gtaaatattt gctaaatcag ataatttatc aattaaatct
      181 aaaataagcg cactgtaatt gacagctatt gcagaacgat aatttccctg ataaaaacta
      241 cttctaactt cttctaataa tggaataatt ctaggatttt taactttttt tatttcatca
      301 tctaatataa acatataaat ctcctttttt tatttgtatg acaagtttac cataaaaaaa
      361 aattaaaaag cgagacatat ttatgttgtt gtgttatcat aataacataa atatgtagag
      421 tgagatgatg aaaaaatgga tttgaaacaa tcgttttaca tctggttata tcctgataat
      481 cctatgtcaa taatggctga tatttttgta gttagcatct tattgtattt attttatcta
      541 attttctttc acaatttaat cgggtacatg attcttgatt ggaaaggaat gttcgatata
      601 gaaagaaaac catttgctaa caatcaaacc tataccgtct tcgaaagtgg agattgggaa
      661 gaaaatgaag ttgatgtgat ccatgaaatt ggattagctt tatcaggaat acaagatgct
      721 gatgttttgg tatcaatgac aacggatact ggatacaacg cttatgaggt attttatctt
      781 atgtatttta tatggcgtga tggtcaagaa attatcaatg gtcgtgctga aaaaacaatc
      841 aattctaata ctgaaatttc tttttgtatg ggtgaagggg aaatgattgt atataaagat
      901 gggtcagcaa acattaaaac agcctttggt gatattgaaa caagtattaa ggaacttagg
      961 gaatatgtgc agaacccata taattttcaa tatttagcag gaagaataaa tttagtttag
     1021 gagtaaaaat atgaaaaaaa ttataaatca accaacaaat cacttgataa ttgaaggtgt
     1081 tattttcgta acaatgtatt ttactgtagg aaatggcatc tcaaatacaa tcattcaaaa
     1141 aaatccacaa tacctattat ttagtcttta tgcttggatt gtacaaatgg tttgggtttc
     1201 catattgatg ctgttgtttt attggatagt acgggttatt cttttattgt taaacaaaag
     1261 aaaaaaggat aggtgaataa aatgaaatca actaaattga aggttgtatt gttatcgaca
     1321 gcagcattaa taacccttac agcatgtggt acaactaata gtgctaataa taacaaagac
     1381 acaaagtcta gtagtacgat ggttactaaa aacagcacta catcggcatc aacaacaatg
     1441 tcttcaatga ctgaaaataa tgctgaaaag tatcaaaaat acgaagacat taataaaggt
     1501 caaaagccgg atgaagttta taacaacttt attaatacag taaaaaatga tgaaaattat
     1561 gggaatgatc cattgcaaaa taaaaatctg actatcgttc tttacaaacc tgattgtcca
     1621 gattgtcaaa aagcagaatc aaaaatcatg caagaaataa aagaaggatt tagttatgct
     1681 ccaacattaa tgcatgatat taccataaat aaaccagctt acgagtatta cgcagttgct
     1741 gtagatatta ctgatggtgt acctgagtgg gtacaaaaca ttcctggtct ggctgatgag
     1801 aacaatcaat atcatacgcc aacagttgca gtagtaacac caaagaaagt taacggcaca
     1861 ctatactggg atgtaatggg aatgacaaca aatacaactg atgaaatgat tgaaagtaca
     1921 actaaatatt atatggattt gggagcatat aaattatatc aacaatcaga gcctgttatg
     1981 gacgatcaat ccaaagttaa atagatcttt agggcgtaag ccctattttt ttaaaattaa
     2041 gtgataacaa ataaatgatt tctgaaacaa acgttcttac tcaactactc gaaacacaca
     2101 agctaattaa ctagattata aaggagaatg cgtaatgaaa gaaatcagtg caaatgaagc
     2161 tcatattccg caaaagaatc aggttggtat atataaggtg cttcatggtg aaggaattta
     2221 taaaatacag ttacttaccg gttacaataa tggtgcaaat gggaagtggt taaaggcaac
     2281 aagtattcgt gaacttagaa gtgaagtaga aaaaattagg cttggtctca caagtgaaga
     2341 aatagctaaa ctgaagggaa ataattcttt tgtaattgaa aaagaaaagg aaaaacatct
     2401 actttatcgt gagctgaaaa ttgaagaata ctatgaaatt gatttacctg aaaaattaag
     2461 aattattgat caacgttcag aagaaaaatg gaaaactgaa atatatccaa gcttaaatga
     2521 ctacaagaca tcaggaaagc ccatgtataa ctacacaggc tttttaacct acacacaata
     2581 caaatatgcc tttcgcaaat taaaaggcat taaacaagct gaaattgctc tgtatgagat
     2641 gaaaaaagtg gatagattga aattgattca agatttagtg caaaatacat ggaatcaagc
     2701 gattgtcgag ctagaaaaag aaaatgggaa aattttaact aataaatatc tgatcgtaaa
     2761 tgaaattgaa gaattaattg gtttattttc atgtgaaaaa ggtgaagtct tcttgaagtc
     2821 atcaccaacc tattcaattt tggacagtcc gtatcagttt atacaggcaa ggagatgagt
     2881 tagatgtcta gcttgaaaga tgactatatc caagcaaaac aagagtatga aaaaatacag
     2941 atgttgaaat ctgagaaagt cgaattattt ttaacacaaa aattagcaga ggatcatatt
     3001 gctaacttag atgctaaaac tttagaggaa tatgatcggc agtttaatca gatacatcac
     3061 aatatcattt tgcttgttga gcaggcaaag cgaaggtttg aaaaagcgga atatgatcta
     3121 ttagatcaag gaattgtatg cttggataag ggattgcgaa gatattatgg tgcagagtta
     3181 aaaaagcaaa atgtagatag aaatgcacta aaacatgaaa tcttagaatt gataatgcaa
     3241 cgaatttgaa gggatagaaa gcaaatgtca caaaacatta gtgctgtaaa acaattaata
     3301 gaagaagatt tagagaccac agttcagaga ttagcggaac gaatccattt tgcaaattat
     3361 caaaccatct ctaactggtc aagcaggaac agaaatccgg aagaaattcc aatgaaattt
     3421 ttttatcgaa tcggaaaaga atatggtgtt gatccgataa aaattttcga gaagaccatg
     3481 atttattact atcatggaac acctaacgaa gtgttagaac atcttttgga agaggcaaaa
     3541 gaaaaatcat caatattaga gctatcgcaa aatgacaaag ttattcaagt gaaatgcgtt
     3601 gatccaacag gaaagattat tgtcggtttt gatattgaaa gaaaatccaa aatagatttt
     3661 attacacaat taaaaaagat aaataattta gatattctaa cgcttagacc agatcatgaa
     3721 cagttgttta tgagttttaa aaaacaattg aaagaaatac aaaaaagata taacaaggtg
     3781 tttcctaatg cgtgaaaagc tcaaagaaat cagagttttt tggataatta acaaagaatg
     3841 tatcatctta tatactcttg gatttcttag catgatcttg ttttggtggt tatatttaaa
     3901 acagtaattg gagagggtca agaggatttt tatcaagata aggatgtgga aactgcatgt
     3961 ggaataatag attaaaaaca ggattgttat taatagttat ttcctgcgct atgatgattg
     4021 gtatgaggat acaaagggaa caatcctatt ttgaagtttc tgctaacaac gtaatagaaa
     4081 aatgctatta cggtcaacat tattggagtg aggaagtcag agaaaatata gatcgtgaat
     4141 atgttcaaag aattgttaga ttaccagtaa gtgaactaaa aaaatgatga gattatcagc
     4201 atatctttta atgtttttga atgtggtcac gttaatcttt gtagtaatcg gtgtgacttt
     4261 cgatttaatg gaatagtggg aaatctataa tggaaaaaag agacacgaat gaacatattt
     4321 tttgaatcaa tattgttgat ggtttttgcg atcagcgctt accagaaatc taaaaaagag
     4381 taatgattgt ttaatcatca ctcttttttt gtagggaaat tgaaatatgt ggagcttgta
     4441 ttggtttagt taggattgct tggctataag gagctagttc tttatcaaaa ccatcaaagt
     4501 aggttttcag tagcgattta actgatcgct catcttgttt gatttggata atttcagcat
     4561 ccaaatcggt tgtgagatca ggagcttttg atgcgattac ctgatttttt ttatcttcca
     4621 actctcttag gctgtttaac aagttgtaaa aagaatcatt ttgattgttc attccaacgt
     4681 gagttgcaat tttaacagaa gtttcgtccg tattgtcatc aaaagaactc attggaatat
     4741 caagtagtaa cgaggatgtt tcttttactg actttttctt tacagcattt tttgtttttc
     4801 catctttttt gctctttact cgagatgttt ttggagctag tttaggtaaa tcacttgttt
     4861 tatcagtcat ttattagctt ttcctttcta ttaatttatg ttattatatt atcataataa
     4921 cataaaaaag tacacttttt gggggcgtag ttattgaaaa aatctaagag aaagattgcc
     4981 acattgctta tgttaatggt gcaatttaca gggattgcaa tgagtgtaat cacaataaat
     5041 ccgcaagtga tacatgctga tgggatagct acttatgatg cacaatttaa ctgtactact
     5101 gaaggtagtg ggtcaggaac agatagcgat ggaaacgata ctggaagtgg cgctgtaaca
     5161 ggtgatgtag ccaaaaatat tgaagatatt tacaaataca tgaatggaga atatggctta
     5221 tcaggtgcta gtattgcagg tattttaggt aactggaaac aagaatcagg tattgatcct
     5281 cgagcagtag aagggtatta cggagatgga cagagagaag ttgacttcat caaacaattt
     5341 actgatggtc tgatgggtat tggctatggt caatggacag ctgggcgaca tacagcatta
     5401 gttgattggt ctaagaaaaa taatggcggt tcttggtgga ctacagaagc ccaattggac
     5461 tttatgttca aaggtgatgg gggattcgta ggaatattaa aagattatgc tttaaatgct
     5521 tctgatgatg tcgtagaaaa tgcagtaact tttcataaaa actgggaaat ttcagctgat
     5581 tccgaatcag ctgtaatagc tgtacgtggt gaaaatgcaa aagaaatctg gaagtacatg
     5641 aaaagcaagg gtatggacgg tgcaaaagat gaaagtaaga tcaataaaat atccggaggt
     5701 tcaagtaccg gagatggatc gaataattca tcaagttcag catcaggaac agtagaagat
     5761 gcctgtgcaa ctacggatga cgctaattct ggttctggat caattgggaa agaaggaaaa
     5821 attaacggta aaaaaggtca agtaatagcg aatatgactt atgaagaagc tttgaaaaaa
     5881 tatgggaaag cgatcacgct accaaaattc aaagaaccag attggtcaac ttcacctttt
     5941 aacggaatat atcaaggtca gtgtacagag ttgacgtggg cttatatgag ccaattatac
     6001 gatgggaatc aaccgactac tggaaatggt ggttatgtat gggaaagcta caaatcggca
     6061 ggagcaaaaa taacgaacaa accaatcgta ggttatgggt ttagtgcttc agatggatgg
     6121 ttatgggctg ttggcgatgc agggcatact ggtgtagtta tagcggtgtt tgaagatggg
     6181 tcatttctat gtgctaactt taatgttcca ccaacccaag ctccaacaag gggagtttcg
     6241 gtaactttga ttgatggagt ggacggatca gataatgtgc atttctttag cggtgtagga
     6301 aaagctaaat ttgaaatcga aaaataaatt ggaatgaagg acgatataga tgaatagtaa
     6361 agtgaaagat acaattatca tttctttgct ttctggtatg ttattagcac tgcttgttgg
     6421 aatcctattc ttcaataagc agaatcagga catagaagct gaaaaacaca gtgtgcatat
     6481 tgaagataag ctgacattct ttaaagaggt taaaccaaat gaggtggatg gattacggaa
     6541 agatttagaa aatgatattc aagaaatgtt gaatggatca gttcaaaata agtttgcaga
     6601 tcaatcaggt gaagaatttt tgagaaaact atttattgta acaggtaaac caccaattac
     6661 tgataagagt agtgagaaag aaaaaatcga gtactataag ccttttactt ttaagctaac
     6721 aaactttgga attaagacat ctagtagtgg gttgtatgaa gcattgaccg attttgaagt
     6781 tcaatacaat ggaaaaaaag tgtatgatac cacattaacc atcacactaa ataatgataa
     6841 gaaagtgact gggggtgctt tttatgcaaa atcatgaaat cactactgct aaaaaggcga
     6901 tcggaatact ttccataatt gtgatccttt gttgtgtggt actatttcaa aagaaaaatg
     6961 cttatgataa taatgtcaaa gaattggaag accttaaaag taaacaaatc gaagtagtca
     7021 agaataacca gaaagtgaaa gctatgcagg aagctcaata caaagaattg gggaatgctg
     7081 aaatcaagga acaaagtagg aaattcttta atttatttta caactggaat tcatgggaaa
     7141 aatatacgaa gaatatgaag gaaatacaaa aagaatttcc tcagatagta aagtctagtc
     7201 ctgttgatat ttctggcacg atggtaggca ctggtaatag tccaatatct agctatactg
     7261 ctgattacta tacaacaaat caaaaaggta aaatggttga attgattaca cagacaagaa
     7321 atactccaac aacagcaaca acaacaattt ggcgtggagt aacaagcata ggagaaaatc
     7381 aattgtttta cttggaggat ttagatagct atgtaagagc ggagtaaaat aaaaaaagag
     7441 ctaacggtac caacgttagc tctttgtgct gacgatgata acaaaaccta ccgaaaaagg
     7501 attgtttcat cacaaacaca aacagaaaag aattgataaa aaacccgaac ctcagaattt
     7561 ttattaatca attcatttag attataataa attgagctga acattacaat acgtatatgt
     7621 tgtaaaacgt aaaaaaagtc aaaagtaaat actttcttaa aaagtcaaat aaaactatta
     7681 aagacaatat atatggttta tttgacataa gaaaatggtc tagatgataa ttgtaatata
     7741 gaattgtagg cttaaataaa gcataaaata ccttttatga taaggtatag ctcttaattt
     7801 taagcagttt ttagatataa gagtgaaagt aaagtggggg agaataataa tggcagatcg
     7861 taaattagaa aagttattag aagaaacgtg gaatccaaaa gaatttagtg aattttttat
     7921 ggagaatttt gaaactgact tagcagtgat tgttaaagat gctcttagag agcaagggta
     7981 tcctgaaact gcaaactata taaacattaa ttttactttg tatactgaaa ataaaggaac
     8041 ttgggatttt tgggctactt tagcaaataa ggagttaagt gataaatcag acacaggaat
     8101 tcgtaacttt tttgaatcaa acagagatga ttatatgtat gctaaccatc aagataaatt
     8161 gaactttaga gtagagtttg atgaaacacc tgaagaattt atagaaagac aaccgcctaa
     8221 agaaaatgta gcaaaggtat tggaagatcg ttggaattca gatgaaatag tttcaacaat
     8281 tagcgaacta ggcggacaat atgaaccgct agtagaagcg gttagagaag agctaagact
     8341 gaataagttt cctgatgttc aaaatattga tgtttctcaa attgaaataa acgtcaagat
     8401 cacgaataaa cttgattatg gtagttgggc agatatagca ttagaaaaat acatctactc
     8461 aacgttaaaa gagtttatcg aaaatagaat ggatatcatg tatctacagc atccacagta
     8521 tttaaacttt ggtgttgaaa tagctacacc tcttgaagaa tggaaaatgg agcaagggct
     8581 tgactagcct ttgctccatt tttttatttt acaaatgaat acagttcgtc gtttttacca
     8641 agaatgattg tttgcccaac tttgagattg tcaggtgatc cttcagcggt ttgtaaggag
     8701 aacgttccgt tgaacttatc ggataccgtt ataggtagct ttaattctga tccttcctca
     8761 tctttgagaa ttgctgtttt tcgttcatca aaataaacag tctttattaa ataatttttc
     8821 gtagtgatct cagcagattt gtcttttttt gtagtagtaa cagtttcttt agaattagag
     8881 gtattttcta cgactattcc atctttggca tttgagcttg tgtttatttg attgaatatt
     8941 ttcagcgaca agataccgct aagaatagtc aatatagaaa caagtgtaat taatactgat
     9001 aatttccttt ttttgttcat tttttcttaa cttccttttt gttttgatta gttagatatt
     9061 ccttatctaa caagcctttt taggctatta aatgaagata aaccccaaaa aaatattgca
     9121 attatgacag tgcaatggta ctattattat acaataaatt ttgcgaaagg ggtaggtcta
     9181 aatcgcaaac aaaaagaaat tgaacctaga caaatacaac ttgtacaccg caagggaagc
     9241 aagtttgatt ttgggtaaag gtagaaatta tgttacaaac ataatcaact attatccaaa
     9301 tcgtttgcct aaaggttcgt tcaagaaagt gggtgacaaa acctatatta taacggaaga
     9361 aggaatagaa gcattgcgat cacaaacaga aaataaaaac aaaaagaagg aatgaatatg
     9421 aagaaaaatt cgcttggtat ggttttattt gcatcagctg tacttgcggg gttatcagta
     9481 ccagtaacct cacatgctga tgaatggact gcaaacacac cggatcaaat ccaaattaca
     9541 acaggacaat caagttatgc gttcaaaaaa ggagatacta tttggtcaac tgtagagaag
     9601 cttaaaagct taggttacaa gactactgtt gctgagtttg ccgaattgaa caatgttgat
     9661 ttggcaaaag gagaacaacg gactatccca attggacgaa ctttcaacat cagtaaagat
     9721 acattaacag ttaaggaagc agatggcgaa gtagttagtc aaactgttca aaaagataaa
     9781 cctgatcctg ttgaaaatac ggagcaagag aacaaaaaat ccgagactgc aaaagaatcg
     9841 gaaccaacag caaacacttc tgataagaca gaaacaagtg cttcaagtgt tgatggagca
     9901 agttcatcta atacaaataa ttctacaagt gagaaagaag catcacaggg aacaagtgaa
     9961 agtagcacta cggaaactga tactggaaca tcaacaaata actcacaatc aacatcaaat
    10021 gatgcaaatg aatcaaccag tacatcttct agtgatcaat cacaaacagg ttcgagtgat
    10081 gaaactgaca gttctacttc accaagtaaa cccgaacaac caactaatgt tgtgctaggc
    10141 gttccattta ttagtcaagg taacacaatg ctttgcgaag gaacatcttt gctagaagcg
    10201 ttacattata aaggtgttgc aacaaatcaa gatttaatga cttttgttaa atcaatgcca
    10261 ctatctcctg ataataatcc aaataatggt ttttctggtg aatggagaca caatgtggat
    10321 ggaacttatc aaggtatgaa tccggctcct gtagtttcat ggggtgttgc taatggcgga
    10381 aatgttgtag atatttctgg ttcatcggta caaggattaa aagatgagat tgtaaatgga
    10441 aatcctgtag tagcgtgggt aacttatgcc tttgaacctg ctcaacataa atcaatgttt
    10501 tggggcgatg ctatttggaa tcgacatgta gtagccgttg atggatttaa agacggtttt
    10561 taccatgttg tcgatcctgt gtttggggca agttggatta atgcaggatc atttgaaaca
    10621 gcatataacg tatctcaatt agcggtttcg gtacgttaat aaaagagatt gtttatatac
    10681 taagtagtta agtatagtct ttatttgtcc ttgcttaact acttagaaaa cttgttgctc
    10741 atggtgggta gaactaaaag gggtgatgct atgaatacta gagcgttgaa acagcttgct
    10801 ttcaacgaaa aaagtgatta ataggtgtgg tgattttaac ttaggaatgg gcgcgaaggt
    10861 cattaccaca ccgacatatc gtggctcatt ttcttcgcct aactggtata aagaaggagt
    10921 aacgagagat gaagcatgaa aaaacgataa ttttaaaatc aaaaataatt tattgttttg
    10981 cgactttagt aatggtgtta tctacgctta taatgccttt ttcagcaata agagctgaag
    11041 cagatgtcac aaaaccgaca agtgcaaaat ttacagaaac tggcgtaaaa acagacggtt
    11101 atattagggt taatatgccg aatcatccaa atgaatggat gatttcaagt caatttaaag
    11161 attctcatgg aaatattgga tattgtatgg attcagaatt gccatcccca acaggaagtg
    11221 gagccggaag cttgaagtac aagggagctg gcagtgatga gttctatcgt atgtttaaag
    11281 gtggttttcc atcaaaaaca gcaaaggaac tgggtgcagg aaatgataca gaagcatggt
    11341 atgcaacaca attagtttca tgggttttag ccggaaactt taaagtttca cagattgtat
    11401 ggtcacatcc aaaccatacc gccgcagaaa cagctcgtgt aaaaaaagca tttgaaaaaa
    11461 tttatgacta tgctaagaat ggaaaagata cgcccaacac tgagttttct atcacagctt
    11521 ctaagacagc tgacgaagga aaatatcaca ctttcacata taaaacagca tcaaataaga
    11581 ctggtaatgc gaagctcact tttacatcag caaaacctgc tggtatgaag atttatgatg
    11641 cagatggtaa agaaattact aacaacactg taaaattaaa ttcaagtttt actattaaag
    11701 tacctgtgac tacaccaagt ggtactttgt catttaaagg tactgctaat gttagcacga
    11761 ccaatccttt tacttttgat ggacgaggtg tgtatcaaga tgctgtagtt atgatcacta
    11821 cttcagaaac aaaggactct aaatctttga gtgctaaatg gacaagagct gttggtcagg
    11881 taacagttaa gaaagttgat caagatggaa atgctttaaa aggagcggag ttcactctaa
    11941 ctgacaataa caatgctaaa tctgttaaga caaccggaga tgatgggtta gctacgtttg
    12001 atttgcaaca aggcttaaca tacagcttag aagaaacaaa agttcctgaa ggttatcaag
    12061 gttcatttaa aaaggatggt attactgtta ataccgatgg acaagaattt agttacactg
    12121 ctacgaacac aattaaaaaa ggtaaaataa aagttaaaaa ggttgaccaa gatggaaaac
    12181 cattagctaa tgctgaattt accttgacgg atgattcatc ccaaacacaa gcagaaaaaa
    12241 cagatgaaaa cggacttgca agcttcgata ttgtagcaaa caagacctac agccttgaag
    12301 agaccggagt accaactggt tatacaggcg cattcaagca agaaggaatc accgttaaag
    12361 atgatggaca aacttttgaa tatacagcta caaacacttt gaaaaaaggt gtagtaaaag
    12421 ttcataaagt cgatcaagat ggaaaacctt tggaaaatgc agagttcact ttaaccgatg
    12481 aaaaaggtgc aacacaagta gaaaaaacag atgaaaatgg acttgcaagc ttcaatattg
    12541 tagcaaacaa gacgtatagc ctagaagaaa ccggagtacc aactggttat acaggcgcat
    12601 tcaagcaaga aggaattacc gttaaagatg atggacaaac ttttgaatat acagctacaa
    12661 acactttgaa aaaaggtgta gtaaaagttc ataaagtcga tcaagatgga aaacctttag
    12721 cagatgcaga attcacctta acggatgaaa aaggtgtaaa agaagttaaa aagacggatg
    12781 caactggtct ggctacgttc aacattattg cgaacaaaac ttacaactta gaagaaacaa
    12841 aagtaccaac aggttacaca ggcgcattca aacaagaagg aattaccgtc aaagatgatg
    12901 gacaagtctt tgaatataca gctaaaaaca ctttgaaaaa aggtgtgatc aaagttcata
    12961 aagtggatga tcaagggaaa gcattagcaa acgcagaatt cactttgacg gacgacaaag
    13021 gtgtaaaagt tgtacaaaaa acggacagca atggcttggc aacatttaat attgttgcaa
    13081 acaaaacgta caaaatcgaa gaaacacaaa accctaatgg ttacacagga aaatggtcta
    13141 aagaaggcat cactgttgct gacgatggac aaatttttga atatacggta attaaccatc
    13201 gtgtacctga aacagtccgc acaggaggaa tcggtacatc agcttttctt cttgtatttc
    13261 tagttgcagg attgggcatg gggttgttgt attatcgaga acaacgcaaa gtaaaataaa
    13321 aaatcggtaa tggaaaagaa gtaaatatga aaaaagaagg ctgacgtatg aaatcgaaaa
    13381 aaattttttc aaatctaatg ctatttggta cattgctagg agtgggattg ggcgcaagct
    13441 caatctcttc tagttttgga cttggggagc aagtagcata tgcacaacaa aactataatt
    13501 atcaaggaac tggaactatt catatccata aagtaacaaa tactaatggg gatgggaaac
    13561 ctgtctcaga tattgataac aaagggaaag aaatagcttt accaaatggt gtacaacctc
    13621 ttgaaggcgt tgagttctct tatgtgaaac ttactaaaga tgaagctaaa tccttgggat
    13681 ggacagatag tgatactaca aaaggtcaac atattgatga tgctaaagca cgattattac
    13741 taaacaaaaa atacagtgaa gtaagtagaa tgaagactga ttcacaaggg aatatagaca
    13801 tacctgtaca agtaaataca gacgatttaa cagataactt gtatttgatt gttgaaacag
    13861 gaacagtcaa aggtgtagtt gaagaagcgt atcctatgat tgttaacgta cctgaactaa
    13921 acggatcaga tgatcaatac tttaattatg atgtacatgt ctatcctaaa aactatttcc
    13981 aattaaactc agcagaactt gataaaaaag acgaagatgg aaaggctctt gccggaactg
    14041 tttttgaatt gcgaaaagat gatgggtctg atcaggtcgt tggaacttat aaaacagata
    14101 gcactggtaa attaactgta gacggtttgg aattaggtga ctattatttc ctagaaacag
    14161 atttgggaac ggatgctaat aaaacaacct atttactaga tggaaaaaaa ttcaaattta
    14221 gcgttaaacg tggggagact gctaaagtag ttcttgatgt taccaactat aaacgtcctg
    14281 taattactaa ggtgattgat aaagaaactg ttgatactgg tgaagatgtt cagtatacat
    14341 tgaaaccatc tattcctggt aatattggag attacgaaaa atacgttgtc aaagatgtac
    14401 taggagaaga gttgacttat gattcttcag aagtgataga aggaatgact ttagatactg
    14461 actacagcat cgagcgtgac ggacaaacga caaactatgt ctttactgat tctggtatgg
    14521 ataaacttca gaaaaaattt gatgcagggg atcgcttctt tacaatcttg atgaatgctc
    14581 acacgaatag taaagctaaa atggatacac cacttaaaaa tgacgtttca acaatctaca
    14641 acaatggata tgagcctgaa gacgaaacgc cagttgatca tgcttataca atcattggtg
    14701 accaccaatt cattaaaaca gatggggata caagcaaagc gttgagtgga gcgaaattcg
    14761 tagtgaagaa caaaattgat cctaacagag ttacttacct gaaacaagac ccaacaacga
    14821 aagctattac ttgggttgaa gataaatcga aagctactga attcgtatct ggtacagacg
    14881 gtaagttcca agttgaaggt ttgcaatata cctatgatgt agattttgat aaacaaggta
    14941 atccaacaat tacagataaa aaaatcaatg attatgctct tgaagaagtt gttgctcctt
    15001 ctggatatac accaacacga aatgatgttc catttgtagt atctaatgat gatgcttcaa
    15061 gtaaagtgac agacataaaa aacctgaaaa ctcctgaaac agttcgtaca ggaggaattg
    15121 gaacagtagt attcctagtg gttgcaggtg tcgcaggaac agggttgtat gttgtttaca
    15181 aacgggaaca acataaagct taattattaa aaatccccta gagtgttttt ggctctaggg
    15241 tttcttttta aaaagagtgt gaaaagtagg tatgagtatg aaaaaaaaga atatcaagct
    15301 aatcatctta agaattggga tgttgctatg tctaggagta ggtctatacc ctgtaattac
    15361 tcaggcttat acctattacc agactcaaca aacgttaaat gcctataagg aaaaatatga
    15421 tgaagcttct gaaaaggaga atgaagaaat cttcaaaaaa tattcggaaa tcaataggaa
    15481 acgaaaagaa acagaacaaa ccttagttac gattcctgaa gataataaca atattccgtc
    15541 aaatggaaat cttcaaaagg ataaattggg tgaattagta ggtgagattc ttataccaaa
    15601 aataaatgtg gatttaccga tttataatgg cgtaaacgat tttcaattat cagcaggagc
    15661 aggaatactt actggaactg attatccgac cggtgggatt ggaaaccatt ctgtcatatc
    15721 agctcattca ggtattccta ataaaaaata ttttacagat ttagaattgc tcaaacttgg
    15781 tgataaattc tttatcaatg ttgcaggaaa aaaacttgct tataaagtag atcggataaa
    15841 aacaattcta cctaccgaat ttgaggattt gcttcctgta gcagggaaag attatgtaac
    15901 gcttatgacg tgtactcctg ttggagaaaa ttctcatagg ttacttgtta gaggagttag
    15961 aataccctac aaaaaaaata ttgttgagaa agaaaaaaag aaagaagact taaaagtaaa
    16021 ggcgcagata aactggtatc ccatcatagt tgtaggtgtt ttatcaataa tagctttaat
    16081 agtcattatt aaaaaaatag tggcaaagag aagagggatg aaaaatgaag aataaaatac
    16141 tacttttttt gactcttatt tgcactttgg ttttaacaat ttatcctgca aatcagctct
    16201 atcatcaatt taaaacgaaa gagaatgtgg cggaaatcca agagaaaatt gataagaagc
    16261 aaactccttc aaaagaagaa gtagcaaaaa atgtcggaca aatactatcg aataacgaga
    16321 accaaaaagt aaacgaaaca caggagtcag ataacgaatc atttgatatg gatgaagcag
    16381 aaggaaaagt caatccatat acaggagatg ttttttccaa gaaccaaacg aaagattttg
    16441 aaccatctac ctattcagga gcggttggct atatatatat tccaaagatc aatgtgctag
    16501 agcttttgtt tctaggcgct tcacaggaac atttatatcg aggtgtggcg caaatgagtg
    16561 ctactagcct accgttatca ggtaaagggc atcaaacagt aatagcagga catcgtggag
    16621 gttatacgtc accacaattt ctttacgttg atcagttagt agcaggagat cacatttata
    16681 tagattatct cggcgaaaaa gcagtctata cagtttttcg atcagatgtt attaaaaatt
    16741 acggtcagaa ttcaacgctg actgatattc ctgatttgga tatgttaaca ctgtatactt
    16801 gtcacccttt tcctcaaaac tatgatcgtt tattagtcag aagcctaaga gatgataaag
    16861 caaaatacat cacgccacaa gtaacagttg ggaataccgc atatgctgat gccattgata
    16921 gtgaatccgt ttcagctaag ccagaaactg ttacatcttc taatgattta tttgaggatg
    16981 ttgatcgtgc aaacaaggaa ttagatggaa ataatgatct taatattccg gaacaaacta
    17041 gcgtagagat gcaagacaga acgtttgctg aaaaggcaag ttcaatattt acaggaatta
    17101 ccaataaaca tcagaaaatg cttttagtca tttggactgt attaggagca ttagcaagtc
    17161 taaaggttct tcttctttta atcaaagaat ttagaaaagc ataatgaaga ttataaaaat
    17221 tgatagagta cagtaaaaag gtattgatac tattattgat ctctttttta ttttgaatag
    17281 agctagattc acttttaaat aaaatcaaag ctgatcttaa ttccaaagaa aaagtcaaga
    17341 aaaaaataaa acaatttttt taatcagaat atttatcaga aaattattag aaaagataat
    17401 cttaaaagct taatttaata ggattattgg ttgtatttaa aaataatgta tggaaaagct
    17461 ataaatcaat agataagatg cagaaaatct tatttgtgaa tgaaaaacaa gtcatcgtaa
    17521 aatattaata ggaggtgtag taatggtggt aatatcaaaa acagcattag actcattagc
    17581 taaagtattt agtagcaggt ttgatggtgc agaatttgtt gaatatctaa gccaaaacga
    17641 aagtggaaaa actctgatcg aaacactgaa aaaagactat ttgaatgaaa atgactttcc
    17701 gacgtggacg aatattgaag atgctacatg ggatgtagtg tgtacgatat attctccttg
    17761 ggtaacgatt gcagattttg ttttagataa aattggaggg gatacatacc ctgatgccaa
    17821 atctcttgct acaagtgact ttaaaacgtt ctatggttat ctaatgggtc taataggcaa
    17881 tggaatagat ttgaaagtta cgccaaaaca aacacctgaa gaaattgaaa aagaaaaagg
    17941 agaggttcaa atggcttatg cagatgacag agaaaaagta aaattattgg aaggtaaatt
    18001 atctgaagaa gaacttggaa aaatggtgca agaaatcaat gaagacagca actattttcc
    18061 tgaattgaag gtatatcaaa acgacgaaga tttctttaat gaaatgttta gtaatagtcc
    18121 atgggaagca gtgagtaaaa caaatttagg agaatggaac tttcaagatg atctagttgc
    18181 atttagcaat gatgggtatt tacgatcata ttcagaaagt gagtatagag ctgaattaaa
    18241 aacgtatgaa acggatattc tgatgagcta tttaaatcta ttagagagtg gtcagggaga
    18301 gttaagagat aaagttgaag aactattaaa agaagagcta tagggattat ttcaacataa
    18361 acacaagcag aaaactacca aacaaaacgt accatgcaaa aagccggtta aaccggcttt
    18421 ttgctctatt atgagaggag atcaaaccat gagtgcaaca accgatatgg ctttagaagc
    18481 agtaataaaa gataatacag gaaatgtcga aaatgtagaa gactttagcg atattgcaat
    18541 caatctttca gcgtggattt cagctgtagg agaagaaaat atcgaatttg agatcaagaa
    18601 tatggatgac ttaaaagcaa aatttccttt tatcgcatcg aaactaagtc aatttgccga
    18661 ggagaaatcg tggaatgaat tagttagttt ttccgaaaaa atttatgata ttacgcaaga
    18721 agatgagtgt gtttcatggg aaatgaataa tcaatacaag aaaattgatg ctttcgtcaa
    18781 atcacgtata gaaggaacag cttttgagtt tgaaggtata gataccgcag ataattggtt
    18841 aaataaagaa tcctttatta aaagtatagc ggatgatctt gaaaaacaat ggtctaagga
    18901 agactatgca gagtacctaa aaacagaaag agatacagag gtgaaagaaa aactcactga
    18961 ggtatgtttg gatgaacata ttgatcccaa tatcgtaaaa caagaagact atgaagtggt
    19021 tattgataaa gttcctactg attttgagga agttgtaaag tatatagttg aagaagcatt
    19081 atcgcaatac tcttcagatg aggaagtgga cgaagaaact gttcgtacca gagtttggca
    19141 actggttttt gatgacaaag attataaata tttgagtgat caaaactcgg ttaattggag
    19201 tgtacgatta ttaattgatc aaaatgatat tgaataaaat agagctgaaa agctctattt
    19261 tttttgataa aaaaactagc tattttttta aaaataattt ggttcaaaat ttattttttt
    19321 aacttgataa ttttttgcca tcctttttta aattggctat atatcaaggt ttatagtggg
    19381 gataaataaa aatacatttg acaaaattta caatatagtt tattatatat ttatcttatt
    19441 atgataacac aataaaagga atgatggtat gaaaaagaag aagtttagtc gatcgttgtt
    19501 aggaatttta gttttagttg cggttggtct tagtggcaag ggttactttc agaataacag
    19561 cactcaggaa catgattttc agatcaaaag tagtgttcag gtaggtaata aagcagacag
    19621 tgaatcaaaa gaatttttag ggatacgtta tgatccaaac aaatatccaa ataatatcgt
    19681 aaacattaat gatgggaaat atacactgac aaaagaagac catgaagcat tatcagaaaa
    19741 aggcagtcaa accttttggg taaattatgc agatttagat caacttggaa gagcaggaga
    19801 tgttgaagcg ttgatcacct atgatgctgt aaattctcac tcatctaaaa atgtggaacg
    19861 tccatctttc aaaagttctg tccatattgc aggagaatac aaggatggag tatttgatca
    19921 agagagagat acttgggtcg gcaaagattc aaacaatgaa atcactcagc tagaaggata
    19981 taaaggttat ctctataaca aaagtcattc attagcatgg tcgctaggag gagatatgga
    20041 ggtacacaat ttaacacttg gaacgagagc gcaaaatgtt gggaaaaatg atcagaatgg
    20101 agggatggct taatcagaaa cgattgtacg tgatgcaatc tataaaaata agaatgaaaa
    20161 agtattgtat aaagttgatc caatttataa agggaatgaa ttggttcctc gtggttctca
    20221 tgtcaaagct ttctcagtta acgataatgg aaaaacggta aaccttaatg tttatgtatt
    20281 taacaatcaa aaaggaatca acatcaacta tcaaaatggt acttgggtaa gagattagaa
    20341 attgagaagg tgaagaattg gaaacaataa gcatcaatgg attagataaa tcagttgcaa
    20401 atcgaataaa aaaaggaaat ggctactatc ttctgaagaa gggtaaatat tcagttgcta
    20461 gagagttaga aacaatgagt atcgtgtatg tagctaagaa tcattttgac gtagaacagg
    20521 ctatttttga tccagattct gaaataggga aatgtgttga attatcagag aaaactgtta
    20581 gtacaaaagg ggcgatgatc attgagagag aaatgttaga cgaaatcaga gtaatcattc
    20641 ctaaagatac gcagatgtgt gaggacagca taagtgacga tgaaccaaaa tcagaaagaa
    20701 aataaggaga tattcagatg agcgaaaaat ccattgaatt aaaggaatat aagcgaagcg
    20761 atggagaaaa atattatcct gtagccagtt tttctaaatc agtatatctg gttggacgtg
    20821 cattggtagt tggtgcaatt ggcatatata ttttgcattg ggtaacaaag gttgtacaac
    20881 tgcttgtaga taccttagta agtattcttg tctcgttaaa tgatgttaac ggaacagatg
    20941 atttaggatt aaaagaaatg tttaaagagt tactcaatgc gttttttccc aattatttaa
    21001 gttattcata taatgtacaa agcttttttg caaagctaca cttggtagac atctctattt
    21061 tagtattaga agcaggaacg gtattctttg ttggatattt ggttactttg atgttcaggc
    21121 atcactatgg agaacaaaga ccattttttg atgatatgga aagtagaagg ttgaaaagag
    21181 aagctctaga agctatggat gtgaagtatg atccaaacaa gattatagga gatgaaacga
    21241 acaacaaaaa aagaaaacga aacaaaacgc aattagaatc cgatgtgcta tcaacgctta
    21301 aaaatatcaa aatttctgtt catacaagac gtgatctaag tgtcaatgaa gacattcgag
    21361 aatatatcgt aaaaatcccg caaccaaaag ggaagaaagt tagagaaaca gttttacgac
    21421 aaattcaaga tttagggtcg atcctgacaa gagcaacaag cggacgagcc aaatttggtg
    21481 aaaaagaaga tgaagtttca ggcaattatt tcattttccg agcatcagta aatattgaaa
    21541 gagaacgtaa ggaaagagta agtaccaaaa acaaagaaac ggaaagagca acatcttcaa
    21601 tcgcagtttc aaattcacaa ttttcttttc caatcaatct ctttgccgat aattccaaaa
    21661 atatccaaaa ccaaaccgaa aaagctagaa aatatgccga aactcaaaag caatctttag
    21721 aactttatct atcttctgct gacgtacctg caagtctaac agatatgcac gttgggaata
    21781 catctattga atatttgttt actttatctc gtaccagaaa aattcaacta agctcaatga
    21841 aagaagaaat tgaaacttac ttgcatatat caggtgtaat cgtgcggatc aacgcagggc
    21901 gtattcaaat tactttacca ttaggagaaa atgatcgtat tccaattgat gtaccatcaa
    21961 tgatcaatag cgtgttttag aagacatcac aaatagaaac agaaaataca aactaccagt
    22021 tcacaaacag aaaagtaccg agaaaagtga ggaatagaaa tggctatgaa attattagag
    22081 aaagtcaaaa agagagccga aaaagctgaa tctattttgc tagataaaga acctaatata
    22141 aagcttaatg gaaaattcag tgtcaatatg ttgaacacaa aggctaaaga tccgacagat
    22201 gctatttttg gaattaagcc tgatggcaca attgctcatt tttgcatcag cgaaatgcca
    22261 cacatgttgg ttgcaggaac gacaggatca gggaaatcag tattactgaa tgaaattctt
    22321 gttacagcaa tgtgtcactc aacgcctgat gaattaaaaa taggaattgt agacccaaaa
    22381 agggttgaat ttggaagata caaaaaattg ccgttcatgt tggctgatcc aattacagat
    22441 atggatgaag cttatgattt ttttgaatat cttgtgatct tgatgcatga acgttacaaa
    22501 ctgatggaaa aagcagggtt acaaaacatc acgctatata acgaatatgc cgaaaaaaat
    22561 ggattagata gatttcccta tgtgatctta ttagttgatg aatattcaca gttggttgga
    22621 acacataaag aagtagaagg tttgattgtt caattaggac aaatggcaag ggcagcaggt
    22681 attcatgtta tcttagctac acaatctcct cgatcaacag ttgtcactgg tattatcaaa
    22741 gccaattttc cgtcacgtgt ttgccttatg gtagcaagtg atttagaaag taggatcgta
    22801 ttagatgaag gcggtggaga aagcctaagt ccaaagggag atatgattat taagctcgtt
    22861 aatggaagta tggttagagc gcagggagct tacatcagta acaaagaaat tgaaacaatt
    22921 ttcaatcacc ttagaaatac tatgccagaa ccagaatatg ttgattaccg agcaatcgta
    22981 gctgaatctc gtggtgaggg tggagaagac gaggattttg gtggaattgg taaagctcct
    23041 gtaagaaaat ctgctaaaag aaatttgaac acacctttag caaattcatc atctaaagag
    23101 ccatcaattg aaaagaagag tacgtttgct ctaaaacttg aaaaaaacaa agaagaaaaa
    23161 gaaaaacaag gagctaaaac actagaaaaa cttcaagccg aaaaattaga aaaagtttta
    23221 aaagcattag ataaagctga ttctgaaccg caaacctcaa acaacacgaa taatgaacag
    23281 ccgatttcat ctaaaactaa accgaaaact actgatccga aagtttcagt aaacgtgacc
    23341 atcacaaccg ataagtctga gaagaaacaa gtaaacggaa cgacttcacc tatcatgcaa
    23401 aaaatgttga aagccaaaaa acaggtgcat aaatgatcat tgtcagttct tttttgaggt
    23461 ggcttacgcc atataaatat acagatgaag tagttgtgaa acgaacgaag aaaagagata
    23521 aaaaagcggt actaactcct aaacagatgc ttgatcatgg cattttaggg gtgaaggttt
    23581 tagaagtgaa taaaccgttt gaatcaaata gtgcaagaga aaagttgctt ggagctgaag
    23641 ccgatgtcgc atatttttac catgacaaaa gcaagcatca aatagctatt aaattcaatc
    23701 caaaatcggc atggcggtat caagcaagag atgacttgaa acgtaggaaa cttagaccaa
    23761 tgctctatcc agaaggaaaa gaagcatttg atagaaccca catcgttccc attggttttc
    23821 atggtacaga atcagataac cgcctattag taggttggaa cagcaatcaa aatagagttc
    23881 agatcaataa atttgagaag aaagtaagga aattcaacga tcgaaaaacg atcctatggt
    23941 ttgcagatat ttgtaaacag ccggatggaa cagcaaagtg ggaagcggtg atatttgacg
    24001 aaaatggtaa aaaagtaatg tctgaaacat ggcatgataa agctaaattt gtttggcagt
    24061 agcagtagag gtgaaaaaga ttgctagaac ttcttgaagc aaatagaaca ggctttaatc
    24121 aaaaattaga agtgggagaa actatagaat ataaagataa aaactatatt attatcggaa
    24181 tctataacac gagaattaac agaataggtg atccacgtat aaccagtgac ttggtttgtc
    24241 aatctgttaa ctattcacct gatctgacaa gcaggtataa gaaatacgtg ttattagaat
    24301 atagacacaa gattactgat gaaattggtg taaacaacac acgaaacata tttaagatcg
    24361 gaacagtgat tccttatagt aacaacgaag aaaaaatatt ttatcagatt tacggaatag
    24421 aaaagtttga atatgaacat gtcgatctgc tagtgacgta ccaaatgcgt ttaatagaac
    24481 cttggtcaca ggtagaaatt gataaagcag taaaattgaa ccgattatca aaatttaacg
    24541 tattaggaaa tgccttttaa aaatcgcaat acacaaacag aaaaatacca aaaaattagc
    24601 aatgaacgta ggtaagaaca tgacaataaa taataataac cctaaaaaat ataatgctct
    24661 tgtccaatgc tatatcgaaa acaaaattac aatgcaagaa gtcgctgatg aattaggcaa
    24721 atcaaagagc ctagtatcac acgattttaa gcacaatgtt tttccgaacc cgattgagct
    24781 gaggaaaaaa tttcaaaaga gagtttatca agaaataggt aagtggattg atgaagggaa
    24841 tgcaattgac cctttaattg aaaaatatcc ttttttaaaa agcaaaaata aaattcaaag
    24901 gttaattcgt caaggcgata ttcaagctaa agaacctgtt ttgacaacca atgatttggc
    24961 taaaataaat cttcataaac aaattattga agactttgaa aaaaatcttt caacaactga
    25021 aattttaaaa aaatataata ttccgagagg aaagctaaca tcgactcttt ttagagaata
    25081 tggagaatcg gcagttacta aagcaaagga agtagcgaat gagagaaaaa aacaaaaata
    25141 gtagtcgata cctagtagcg actgtagttt ttgtttgtgt attgattttc ctgtttgatt
    25201 ttttgcagat tggagaaagc ataattggaa atacattgag tgcaaaggaa atggttgctt
    25261 taggtggttt gtatgaagga cacttgttcc cgaatatatt catttctatc gtttctcata
    25321 ttaacttgta ccatttaatt tctaatttat tgttcttagt agttcttgaa gaagcgctga
    25381 atgatcagta tgagtggtta acattattga tcgttttaat catatcagga tttctaggaa
    25441 gcttagttcc ttttctgcta cacagcaaca tcgtaagtat ggggcttagt ggaggtattt
    25501 ttggatggat tgctttattt gcgattgcat acatccgttt tgatagcaag aaacatttat
    25561 tactaagtgt ttttgtatta ttgataatcg gcattttact ggtatctact tttgaatcag
    25621 gcgagacaaa cgtcttgtca catgtaatcg gatttgtctg tgggatgatc ttgggattta
    25681 ttctaaagaa cgtttcattg atcaaacaaa agaattaaaa gggagtgcta aaaatgccaa
    25741 aaaaaaaaga aaagaaaatt ccagtgtttt tgtctgtctc agaaatgtta tcacttcaaa
    25801 tactgttaag aaaaaagatt gaaacaatag acgaagaagt tagaaataaa agggctatgt
    25861 acgaacaacg tgaagcgcta agagaacgaa tccactattt tgatgatata cacactaaaa
    25921 tctatgaagc acgtacaaat cacgaagacg aaagcaaatt aggattagct caacaacgaa
    25981 tatttgaatg gctgaatacg aatactgagc aagaaccgtt aagagcaatt agtaatcttt
    26041 ttgaatttgg aatgaagcat tggtttaacg ctgtagaaaa ggatgctgac ttacaagaag
    26101 cgttcgatac actaagcgag gatagcaaat ccatgcgaga cttattgatc acttatctaa
    26161 atgagcatga atttgaacag acgaaagtta aaaagaaaaa taaaaaatag aaagttgtga
    26221 tgtatcatgc tgtttacctt attaggttat ttatgtttag ttcttgcttt ttttggagca
    26281 attgctatat ttgtcggtca gtttcaagca aataatccga taatgcgtaa cataggcatt
    26341 ggaattgtta tcatatcctt gattgtattt ggtgccacaa agatttttgg gagtaatgat
    26401 cagcagtttg gtatttctgg agttgataat agttatgctc cagattcatt gggaagagta
    26461 gtgatagatg gaacgtttga gaatgtaacg agctacaaaa atgtttcatt gacagtgaaa
    26521 gaagatgaca agaaagcatc agatgacgtt ctcgctacga tcaagccgtc tcaatcacag
    26581 tttgaagtgt tttatacagt caaagagaat aaccctgtac gcaagctaac attagttttt
    26641 aacgttgacg gagaagaata ctataaagat gtaaaaatca ataaagcatc aacgaaaaag
    26701 attgtcgaat cagcgaaaaa agaagtagct acagaagaaa gtacaactga atcaagttct
    26761 actgtcgaag ttgaactacc agataaaatt gattttgatc ctgaaaaagt caaatcgttt
    26821 gaatctgttt ctggttctat ggcagatcgg tttaacactg aagttttcaa agctatcaaa
    26881 actgttaaat tggatactga aaagcaaagg attaatgtta ccttattcaa ttcagttgaa
    26941 tctatggatc aggataaaaa agttacggtt gcaagagaac taggaaaaag agtaattggt
    27001 ttgtatcgtg gtactgctaa accagattca gacggtgcag atttaccagt tcatgtagtt
    27061 tatgaagacg gtaaagtttt tggagattct acagcaaacg aagcattaac gattaaattg
    27121 gctaaatagc cagaaagtta taataaacaa atggtgtcac accaataata tataggtgtg
    27181 acaccttgga gatgatagct gatggtagta aataaagaaa aagttagaaa gtatgtgact
    27241 ttaaaaatag cttctgttaa atatttagaa aagcgagcta aagaagaaga aagagcaaga
    27301 ggtaagaaag taacagtttc agagttgtta gaagaattga tcgagaatga tcaaacaata
    27361 agacgtgcat ttactaatag gtctatcaga taacaatata attattaaaa aagaaagagt
    27421 gatttggtct tagactaaat cactcttttt atataaagtt tctttacact tattccgaat
    27481 aatgttaaaa ttctttatac gatatatagt tacttgtcag agaaagagat aaccagcaat
    27541 attggaggga taataagtgg aaaacaataa tgatattaga cattttagca ttggggaaag
    27601 agttacaaca gtaagtgaat tatcaaagga ttatagagat aaagagggaa cgattatttc
    27661 tcttaataag aatcgaagag gcgtttggca atatgttgtt gatttaggtg atggcgttac
    27721 agttgagtgt aaagatagtc aactgatcag aaatgatggc agtcattcca ttgaaaaagc
    27781 aattcttgta tacccaaaag tatcaagtaa gatcatgaat gactggttgc ttggaaaatt
    27841 tggtgagtac aataaaaaag aaatcgcaga agtgatcggc aaatcttatc caacagtatt
    27901 agggtatcta aaaaagataa ctaacaaaga agcgaaagtt gaggatgttt taacagtaag
    27961 agaacttcag tcattaatcg aatatcggaa taaacaaata acaaatcctg agtaccttaa
    28021 aaaagaagat ggtctattga aacaattgga acaggtaaat aaaatccgga agatgcaaaa
    28081 ggtagctcct ttaagcctag ataactttaa aaaaatttat ttgaaagaga tcgaaaaaga
    28141 ctaatgtgga gtgtcacttt accgtttcaa cctgtatctg caccaagacc aagtgcaaga
    28201 aggaatgttt ctgatgcaac taatgacgta gcagagaaaa ggatttcaac ctattatgat
    28261 aaaaagtatc tcgattattt gaaaaaaata aaaagcttta tcgaagatca aggtttgtta
    28321 gatgatgaat tttactcgat cgtaaatgat ccgatgggtg caattatgga agtggacttt
    28381 tactatcaga taccaaaaag ctataaaaaa gtctataaca ttatgaagac gacagcgcca
    28441 gacttagata atttagtaaa aagtgctatg gatggtattt tcaacatctc agctatacga
    28501 gatagccata tcacaggctt aattgctttt aaatataatg tggcgaacga aggaaaaaca
    28561 gttgtgagga tcaaacgata ttcagaattt gagtgggata cttctgaagg attgaacgga
    28621 gatatatggg aagctacatt tgatttcaaa ccagtagcaa cgccaagacc aaagtctaaa
    28681 tttagaggaa atgggattat cacttactat ccaaaagagt acaacgacta tttggaaaat
    28741 atcaagaata cgcttaaaga gaaagatttg gtaaatgatc aactgaaaaa ggtaatgaat
    28801 gctcctatgg gggttatagc tcagatagat tatttttacc aagtgagaaa agataagaaa
    28861 aagcttgatt cactgatgcg tacttcacag ccggatattg ataatttagt caaaggaaca
    28921 ttggacggaa tttttaacaa ggaaataggc atcaaagata gcagggttgt agggttaatt
    28981 gcttttaaat acaatacttt tgaaaagtcg aaaacaaacg tgagactaca ggagatgggc
    29041 tgatgaataa taatgaaaaa attcttcatg tacttgatag ctttgaaaca attcaagaag
    29101 aactaaagaa atatcgggat gttttggaac aaagatatga ttttgtcaat cagcaaaaga
    29161 gtaatcacat ggatttcatt ctcaatatga atgacttgaa gaagaaatta gttgaacgaa
    29221 aagaacaaga aaagttgatc aaagcttact ttgaactcgg tgaaaaagaa gttaaaaagg
    29281 caatggaact gaatgaagat cgcagggttt tagatcagct gttagaacaa ttattggtga
    29341 tgtttcaaaa aggacgaata gatgaggatt taattgaaga agggttgaga aagtatcctt
    29401 caaactcagg tatcggaata gtgctgaaag cgattgatga agaagaaatt gaagctttca
    29461 taccgccaga ggattttgaa tcggcaatgg aatatataaa atattatagc caagggatca
    29521 ctgcttttag agagtttgat cctgaagatg ttattcatga cttgaacaac ctaaaagaat
    29581 ggtgtgaaag ttatgaagtc gatgattccg gattggacta cttgatcagt attatggaaa
    29641 tcgaagaaga gatgccggat aagcctgatc caacagatat tttagaattg atccatgaag
    29701 ctagaaatcc aattgcctat atttctagag gatatactgt attggaatac tataagcctt
    29761 atatatcagc gatgaaccat ttaagacgtg tattaaggga aaaacgtgaa taccgctcag
    29821 tgttgaatgc aacgaataga ttagaaaaag ctgtttcaga gctagatgct tattatcgtg
    29881 aacattacct gcaagcaggt ggtatgccaa gaaatacgaa agcaaatatc agtaggtata
    29941 taaaaaaagc tgagtaggcg gagttagccc gtaaaatggc aaaaggtaac ctgaaagggg
    30001 cattttgaat gaaaagggat gtttttgaat tgtacgtatt agaacgtcta gaagacggca
    30061 tgatggcaca agagataatt gatgaaattg atgatatgat cagcagattt ggtcaattgg
    30121 agttgaatga cggaaacaaa aatccggtag tgctaaaaca attttggttg aacaattttt
    30181 cgtataagga ttttatcaaa gaaaaggcaa atacattgta ctgactaaat tcacgaggag
    30241 cagtgatgag agcttattgc taagagcagt taggctcttt tttttgtttg tgtaaaatta
    30301 tttgttttac aatacacatt tatacacaaa aaatattgac ggatagtaca caaatgtggt
    30361 aggatatatt tgtaagcaag ttagattaac aagttaaagg aaggaagagc agttatgaaa
    30421 aaagaagaac taatagatat atttcaaatt gttgaacgag caaataatat gggaattatg
    30481 ttttttgatc gtatttcttt aaagatggat ttaagcgttg cccaccaaga atttaatcta
    30541 cgattaaaag ccttgttgaa ttctgatgat gttaatttcg ctcatgatgt tgtaggcata
    30601 cagaaccata tagatagaga aaacaaacgt atgggtgatg gattcttacc aagatattca
    30661 agtttataat ttgaagaaag attcaagagt atgtgtcatt attgataggt actcttgaat
    30721 tggaaaggag gaatgaattt aagacaacaa cgattatgat cttaaaacaa tcgtttatgt
    30781 aattgacaat cttacgaata attttcatgc aataatgaca ataaaaatga aaaggggtaa
    30841 aatcaaatga aaaaaatttt agtaggaatt ctattcacaa tggggctagt gctattatca
    30901 ccttcagttt cagcagaaag ctctatttcc atgtatcgta tgtacaatcc caatagcgga
    30961 gagcatctat acactagaag cgttggagaa agagacaatc tgaaacgtta tggatggcat
    31021 tatgaaggtg tcgcatggca agctccaaca agcggtactc ctgtttatcg tttgtataat
    31081 cggtataacg gtgaacattt ttacaccatt gatgccggag aaagagataa gatttcgcac
    31141 acaggatgga cgtacgaagg tgttgcgttc tattcctaca acggagcaaa tagagttcct
    31201 gtgacacgct tgtacaacaa atacgtgaat tggcatcatt acacgttaga tcaaaatgaa
    31261 aaaaatatca ttagtcgaca agggtggaaa atcgagggaa ctggttggta tgcaatgcca
    31321 aacagcgcac caaatcctgc acctgctcca agcaatgaaa aagtgttaaa tgttcctgtc
    31381 atttggcaag gtaatacgat gttgtgtgaa ggtgcttccc tgttagaggg attgcactac
    31441 aaaggagtag cgacaaatca aaacttaacc agttttgtaa actatatgcc aagagctaat
    31501 gacaataatc catatcatgg ctattctggc gaatggcgct acaacgtaaa tggtacgtat
    31561 caaggcatga tgcctgatcc agtagttaaa tgggcaaaaa atgttggtgg taatgccgtt
    31621 aatatcactg gttcaggaac gaatgggatc aagaatgaaa ttcgcaatgg taatccggtt
    31681 gtaacttgga ttacttataa ctatgcgacg ccagagttca aacaaatgcc ttgggggcgt
    31741 gccgtttgga atgggcatgt tgttttggta gacggattta agaatggcgc ttaccatgta
    31801 gtcgatcctg tatttggagc aaaatggatc aatgcaggca cgtatgaaaa atcgttcaat
    31861 gtaactggca tggcggttgc tgtacgttaa ttgatagaat taataaataa atgaaataag
    31921 agcttaatta ctgaaatggt aaataggctc ttgtttttta ggaaaaaact gctttttgta
    31981 ataagaatat ataacaaaaa aattgaattt agtacacaaa agtggtatat taaaaccgta
    32041 gagattatgc gaagaggtaa ttttgatgaa ccaaagaaga agaaaaagac tagaagaaat
    32101 aagcgagcga ctatccgagt taaaggatga gttagaagta attcaagaag aagaggaaga
    32161 gtacaaagaa aatatacctg aaaatctaca gggatcagaa agatatgaaa aagcagaaga
    32221 atccgttgat caacttgaag aagctgttga tggtttatcc gacatattag aattgattga
    32281 agatatagct agctagagat gactaatgaa gatttcagga gtgagcaaaa aatgattgat
    32341 atttcactga tagaaaagct acttttttca aataagtaca cagccaaaga gctggctaca
    32401 agatggcaaa tatccgaatc agctattaaa gcctatcgtc aaaataaggg aagcgtagga
    32461 tggcgtgatt ggaaaggaat cagcattgga aaagcagaag agataatgaa acgtgaagga
    32521 tttcaaactg acaatgattc taaaaaagtt gaatagttta atagtcgcca agaaaagtac
    32581 cagggcgaaa ggtagattac aaaggaaaac acttttaaat aaacttcgca aaagcatcag
    32641 gttatgagtg gctagggcta tacgaatgag atgaaaacaa agggctttaa tgtcaaagaa
    32701 aacgcaatag gggggaataa aatttttaaa aaagatattt atgtttggtt taatggaaaa
    32761 aaatatatga ctactaacaa aaggtggtca gagctagtac aaaatacagt taaaaaagtc
    32821 atgccagatg gatatcattt caactatata caatgctcac aaacaactga aagtatttat
    32881 ttttcaatat taaatgagaa tagactctat tatttcaggg taagttcaca ccataggaaa
    32941 aagagggaag ataatgttac gacattttta tcgggaaaat acaatggttt caaagagtta
    33001 agtgaagcaa ttggtgagta tctaaaaagt aatagatttg tcagaatttc tgacaaacat
    33061 atattgatct tgttacttta tttgaataat agcttcaaat tgttttggag agacgacact
    33121 ttctactata tagagaacgg catggatatt tattctggta tcaacggcac attaaaacat
    33181 tatattaagg cgttacttcg atatggggtt ctttatcaag aagatttaag taaccaaatt
    33241 aagattagta attctggaaa gtcattactg gcaaaatata aaaaattgct ttctcctatc
    33301 tacaaaggaa tagatatgga agctgtaaat tctgaaactt tatttaattt agttgttgat
    33361 gaaaagtttg cgaatgaatt ttttaaaagt aaaaaaattg aaagcctaaa taacctgttt
    33421 cctgaaaatg caataaaagc ttatgtagat aataataaat cacgattata caactggttt
    33481 attcccaaac ggttgcgagg aaaaatcaaa attggcgatc aagtgaaagt aattaatcta
    33541 aatcatacta agatcgttac cgtaacggag ttgtatatag ctgaaaaaga agagattaca
    33601 aaaatgaaac cagttattaa aatactagat aacaaagagt gagtggaata tatggtagca
    33661 aaaaagaata ctctaaaaga agcttctgaa aaatatcaag atatgaaaag acgaggatta
    33721 ataccaaagg acaaaaaagg tatatggaga gaatttgtta aggaagtcag tagtgatcga
    33781 agtggcggtg actttgttgg acgatataca gcaaaaggtt atagcaggcg cttaaggatc
    33841 actgttgaat atggaacaaa tgaatcagct acagagctga agcaaaggta cttagataca
    33901 cgaagagaag cttcaaaaaa atatgatgat ttcaagagca gaggtctaat tcctaagaag
    33961 aaggggattt ggcgtgagtt tttcaaagaa gaagatagca atcggaataa gggagacttc
    34021 attggacgat acacagcaaa aggctacact aagaatttag ggatcaaggt tgaatacgga
    34081 actggtgaaa gttcatggga attgaggaac cgctatttaa atacattatt ggagaaatac
    34141 aaagcagaat tattagagct gttcgatgtt aatgatttca tagagatggc tgaaaaatat
    34201 ggtcatgaag aaggctttat gatcaatgac attatggtta ttgataattt tacggactta
    34261 ttaggacttg cgaaaaaagc aagagattta agagaaacac agatgtatct attcagaaat
    34321 aaaaacggaa gaaaccggag aactgaatcg agcgagatcg aagattacta tgatgccttt
    34381 acacacgatc taggagcctt tgtagacgag atcagtggtg ttatggacaa atatagggtc
    34441 tagaataaaa aagccggaga tgagcaaata aatgggaaat aatagcatat atacacctga
    34501 aatgcagaat aaaattaaaa gacttgtcgg attagggtgg atgacaagag gtaagttaga
    34561 ttcttttagt acgatcaaat atcttttgat ggatatctac cgccaaaaca agaaaacagg
    34621 aatacatttt ttgggctatg ataagccgga agatgcgcct ttctttgttc ctagttcagc
    34681 ctatagagcc agtacttccg gtagcatgta taacttggat ttgtttgagg aaggagcaaa
    34741 gatgatcgaa tatgatttta acgaagccta ttcgaggatc atgagaaatt atcctttgcc
    34801 gtcaaacact tatttgaaag caaaatataa tacagatcaa gctattttga gagcgataaa
    34861 tagaccaaaa gtaagaccgt atgaaaattt aaaaacgtat atgttctttt tggttaattt
    34921 tgaaggaaca gccaaagaag gaacgtatgc taatccaaaa tcacagtcga tgtttgcgga
    34981 ttatggcgaa aatcccaaaa cagctaatga gcctatttgg ctatcggaga ttgagctaac
    35041 actaatcctt gattattatg atatcaaaga gatggaagtc ctcgatagtc acacattttt
    35101 gacgaaaaaa ggattactca aagaatattt cgaaagaatc gaaccattaa aaactgatcc
    35161 tgatacgttg aaactttata aaaatcttag aacgaaagta tttggtgcta tcggacaaaa
    35221 atcattaaga gaaggagaaa caaacacctt cgataaatac agtatgtata atcgagcgta
    35281 ttcctccatg gtagcaggcg tatttagaga tcgtatggtt aggtatgaac agaaatacgt
    35341 tcatagcgca tatgggctat taaaaatccg tactgatgga ttgtatttca gatccgaagt
    35401 accagagttt gaagagctgt ataaacttgg agtagttaag aaaaaagttc atacaatatc
    35461 caaagaagaa gtcgaaaaag ctttagaatt taaaaacaaa ggatttgaaa ataagggctc
    35521 ctttgggaac tctaaagatg aagttaaggg tgttgtgata gacgatgatt tcgagtttga
    35581 ttttgactaa atactctttg gaaaagatgg taggaggtaa atatgaagaa gaaaattgtg
    35641 gaaggaaatg taggtgttat tattctaaaa gattcaactg actatgctaa atcaatggca
    35701 ctaaaagacg aaaaagttgc acgcatggat aaagatttag ttgaatttct gatggatgat
    35761 acagaaagat taaaagggga tcagtttttg agtttaatga aagacaaagg cttaaacaca
    35821 tatggtttat ttgattataa gaaattagct atagagtggg taaaagaagg agaaatattt
    35881 tttattgaag aatgggaagg tactgagaac ttagttcttc atcagtatct tttgaaagct
    35941 taatctaagt aaaacagttc tgactagtta tgcttgtcag aatttcaaag gatgttttcc
    36001 ataaccatca tcattcaaaa ataaaggatt tgaaaataag ggttcctttg ggaaatctaa
    36061 agatgaagtt aagggtgttg tgatagacga tgatttcaag tttgattttg actagatttt
    36121 tttgcaatgt ttaagagtaa acaaggtaag aactaagatc atgaagattg agagtaaaga
    36181 agatagagga cgaatgtatg agaacacact atatttattt agaagataaa attgtagatg
    36241 aaattccggc aaaaagtcga gaacaagcta gaaaattatt tattgaaaaa tacaaagaaa
    36301 tcaatggaga tagtgaagtt ccaaaagtgg tcgtaacagt tagaaaaagg caaacaaaaa
    36361 agagaagtac gtttcatgtc agccctgata tatataatct tatagagcga catagaagtg
    36421 aagataatat ttctatatct caatatattg gtacagttat tgatgaattg atttcaggaa
    36481 aatatgacta tgcttttgat tttgagaatg aaaaggatta ccgaagtgtt gctgtctttc
    36541 aatctgattt tgagaagcta aaaaactatg ctggcaggag aaatgttaat tttgcaagac
    36601 tactagaaga gttctactcc gaagagttgg gagtaggaaa tgaatacaag agagaaactt
    36661 atgcgaaatt gtctcataag ttatcccaag cgcttaaaga agtgacagaa gttaaagcgg
    36721 aactagatgc taaacttgaa gaagtgaaaa aatatcaaac agagaggaag aacaacatga
    36781 agaatcaagt ggaatttgac gaacagaaag aatttgaaaa agcaagaaaa cgctataaca
    36841 aaattatgaa tgaacttagc tatgtggatt tgagtcaata cctcggacac gatttgtgag
    36901 aattttttcg agattccagc gggtgagtat ctcatcgttt cgaccagcgg tcaaaccaaa
    36961 accgtttgac cgctgatcga agcgatgaag ttgaaaccct gtggaaatct cgccaaaaaa
    37021 atgtaagtgg tagctaggaa ctaagcttgc aatgccaact cctccatctg atttggtgtt
    37081 aaatagttga tactactatg gattcgcttg cgattgtaaa atccttcaat gtattgaaat
    37141 aacgctaaat aagcagcttc aaagttttca tacgcttttg atggatagac ttcttctttt
    37201 ttgagtgagg cgtgaaacga ttcgataccg gcattatcgt agggacagcc cttacgactg
    37261 aaggagtgcc gaatgttcaa ttcattcagc tttgcctcat aggcttcgct agtgtactga
    37321 gaacccaaat ctgagtggat gatcaagttc tcggagggtg tttggtttaa gagcgcattg
    37381 tcaagggttt cgataaccaa ttctttcgtc atctgcttgc caaacttcca accgatgact
    37441 ttctttgtgt ggaggtcttg aatagtggag aggtaggtcc agccatcttt tagggtatga
    37501 atataggtaa tatctgtcac ccatttttga ttgatcgacg tagtggtaaa atcttggttt
    37561 aacaggtttt tacgtccttc acttttaact ttcgcttgtt tgccaggctt gtacttcttg
    37621 agggtgactg aacgcaagcc taattttctc atcagtattt gtactttctt taaggacaag
    37681 gtctcgccac ggccaacaag aatcttatga attttaggcg caccatagat acccttgttt
    37741 tcaaagtaga ttcctgaaat caagcggttt aagcgtttgt tttctgattg gcgcttggaa
    37801 ggtttctttt tcttccagtc atagtagctg gatttcggtg cttccaaaac ggtacacatt
    37861 tgttttacgt catgatcatg aacatttgat tgaataaatt cgaagacatc actcattttt
    37921 acttttgagc gaatatggtt aaagcctttt ttaggatagt gttttcctcc tgcatgcggg
    37981 ccatttcctt tttcatcgct aggatttctg cctcagtcat gccggttgct acattctttg
    38041 tatgaagctt cttccaacga taaatcgttt gtgtagcgat accatattgc gctgccagtt
    38101 cttcgaccga ttggccagac tggtttaaat ctaagatcat cttcttaaag tcttcatcaa
    38161 attttttctt ggcttttgcc atcgtggaca cgtccttttc ttagtagtta gattctacca
    38221 aatcgtgtcc atgaaatcat cctaacatca atgaacactt aacagttcat gaagaattga
    38281 gtgagggtac agaaaaatat acgatcgcag atatggttaa tgaagtggaa actcttttag
    38341 cttctttctt tgaaaatggt cacgtaaacg atgaatggcg aaaagaagaa cctaaaaggg
    38401 ttaacgctta tattgctcga ttaaaaaggt ttattgataa atataaggag aatagtgaca
    38461 aattttctaa agataaataa ggagagagtg tgattacatt tgccagaaat cgaaggaata
    38521 ttttcgccaa aaataggtaa aaaacagtta gatttcagca gttttccgag cgtaaataga
    38581 gattataatc taggtgatga ggtacagata ctaaatattg atttcaaaga actagacttc
    38641 gctgttgctg tttccaacaa acccaaggcg ttggaacgag tgaaaaaaat ataccaagag
    38701 aatgatcaaa atatcaaaaa tatgatcaaa aagtatagac gagataattc aaaagttgaa
    38761 gacgttttat tcaacggtac ggcaaaacat gatttgaatg tttatatggc attaggagtg
    38821 gctttgtact gtaattcaat taatcagaca gatattttgg ttaatatcgt gaggaatagc
    38881 tatcaaaaaa tattaaaaat aagtagcgaa acaaaccctt attatgcaat gaaatatctt
    38941 ggggataatt tattaaatga taaaaaattg atgcacaaca ctgaagagct gttatctaat
    39001 ttggctgcga ttaactttct attcaaaaca ggttctgaag attattttat agataacgta
    39061 ctaaaaggtg cttacggaac agcattttat ccgctttaca atatgaaaca gcataaagcc
    39121 gaacaaaagg ataacaaact tgaaattaaa aagatgaaag aactattagg tataggattt
    39181 gtaaagccca attcaaaagt cactattgct tcgttagcaa ctactaacaa taatgggaaa
    39241 atagtaaatg ggtttgacga ttcagatata gatatattgt caaaatcata tttttctttg
    39301 tatggagatt ttgcagattt gatgggtatt gatagttact tagtcaataa ttttgagatc
    39361 aagggtagcg agctgaatga tattgtgtat ttcgttttaa tctctctata tgcaattgat
    39421 cagaataccg agtaccgaca aattgactat ttgctagttt tttatagttt gttatatcag
    39481 ttcgttttaa ctcggttgtt taaaaatgct aaggaagaat tgatcctgaa ttctgattat
    39541 gaaatagcaa aacaaattgc aagtattaga aaagaaaaca aatctgaagt aatcaaatta
    39601 agaaaagaat taagtattgc gagtaaagaa aacggtcggt tagaatcttt agtcaaatcg
    39661 gcaaaaaaag aagaggaact tttgaagcaa gagattaaag aattaaaagg aatcagaaaa
    39721 gaacttgaac agctgaggtt tgaaaataaa gtattaaagc aattgcagga gcaagaacat
    39781 agacaatcat taaatggtaa aactgaagag ccttcactgt ctgaaatgat agcttatcta
    39841 aaacataaaa acattctttt agtgggagga catgtaaatt ttcaaaataa attaactgaa
    39901 ctattaccaa atattaaaat gtttgatgta agcgaaaata atggaaagat acttaaaaat
    39961 ttgaatacgt gtgatgcgat ttttctatat acagattact tgaatcatgg tctatattat
    40021 cgtgttatga gtgaaatttc taataaagat aagatatttt atttagataa tcatgtgaat
    40081 atgagcttaa caattaaacg tatttatgat cttttatatg tttataaaca ttaatgaggt
    40141 gttaaaattg acaacaataa ctatgacaaa aaaaatagaa gtatatgttg atggatcaca
    40201 agaagaaagg gattcctttt ttgatttttt ttggaaaaat agtagagaat atgtaaaagc
    40261 gttgaatctg gtatataaac agctaatgtt tgaagagttg ataaaagatg caattaaaga
    40321 aatggatcaa ggttatgctg agagagaaga aagtattcaa aatcggattg aaaagttagc
    40381 atatactgat ccaatagatc aaaaaaaact tgataaagca aaagctgaat tatctaaatt
    40441 gagaaatgaa aaaaataaag aagcgagaga aatattaacg caagcgatag ggttgaagag
    40501 tcaaacgaga acacgagatg tagcaaaaaa attagacttt gactttggag attttgttga
    40561 ttccgcaaat atgaaagcta gtaaagattt tggcaatgat tttatcggaa ttgtgaccgg
    40621 acaaagaacg cctagaatat ataaaaatag aagtaaatat ggattattac cttttagagg
    40681 tcgagatatt aaaatccata aagatgataa gttttatatt tatttaccta aaggctttag
    40741 gtttaaagtg aatttaggta gcaagccaag aaaagcaaga gatgttgctt cagttttaca
    40801 acacattatg gatggcgatt acaaattaaa tcaaagtaat cttatttttg atggaagaaa
    40861 attatttttt aatctaacaa tttcttttga aaaaagatat aatgaactgg agttcaagga
    40921 aggtaatgaa ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt tagttaataa
    40981 taaatcggta atgtcttttg gtgatagcca atatataaga aacttcaaaa gtaaaatttt
    41041 agcaatgaaa tcaaaagaac aaagccgagg agtcttttct aaaggcggtc atggtcgaaa
    41101 aagaaaatta gatcaagatc gatgggaagc aataaagaaa agagaaagta attttgttaa
    41161 aacttataat aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca ataaagctca
    41221 aattattcgt atcattgaac cagataaggc ttatggcgaa tgggcttact atcaattgaa
    41281 agaacaacta gtaaataaag gaaaaagata tgggatagaa gtcagaagtt agtaaagtaa
    41341 acaatcgttg atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc
    41401 cgtcaagttg tgtgaccaga cggaaggacg agcgttgttg gggttttaga accatataaa
    41461 acagcatgtt tctcaaattg tgtttgtttc atttatgtta taataagagt tttagaacca
    41521 tataaaacag catgtttctc aaatgagtac tttaaattaa ttgatggcgt tcctgcttta
    41581 gaaccatata aaacagcata gttctcaaat tgtttaaaat gattgtcaag ttaaaaaaat
    41641 ctgggatcat ataaaatagt atagttctca aatatgatta gaaaccctat taaagagcaa
    41701 tattaagcca acttacttaa atcgtttaat taagaacagt aatctctttt agtcagactt
    41761 ttagtctgac ttttttgtat ttccaaaatt tattctttac aaaaacgaac atatgtttgt
    41821 ataattgctt gtgaatgggg gaataaaaat gtttgattac agtagagaac cggtgaatga
    41881 ctatttttta atcgacatga agtctttcta
//
LOCUS       DAPBAB010000018        38018 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPBAB010000018 DAPBAB010000000
VERSION     DAPBAB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.018"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(428..1372)
                     /locus_tag="RD444_001218"
     CDS             complement(428..1372)
                     /locus_tag="RD444_001218"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDT7548601.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1668..2612)
                     /locus_tag="RD444_001219"
     CDS             complement(1668..2612)
                     /locus_tag="RD444_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548602.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2833..4047
                     /locus_tag="RD444_001220"
     CDS             2833..4047
                     /locus_tag="RD444_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7548603.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4069..6084
                     /locus_tag="RD444_001221"
     CDS             4069..6084
                     /locus_tag="RD444_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HDT7548604.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6167..7540)
                     /locus_tag="RD444_001222"
     CDS             complement(6167..7540)
                     /locus_tag="RD444_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDT7548605.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7566..7850)
                     /locus_tag="RD444_001223"
     CDS             complement(7566..7850)
                     /locus_tag="RD444_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7548606.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7852..9867)
                     /locus_tag="RD444_001224"
     CDS             complement(7852..9867)
                     /locus_tag="RD444_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7548607.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10046..10351)
                     /locus_tag="RD444_001225"
     CDS             complement(10046..10351)
                     /locus_tag="RD444_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDT7548608.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10440..11471)
                     /locus_tag="RD444_001226"
     CDS             complement(10440..11471)
                     /locus_tag="RD444_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7548609.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11678..12553
                     /locus_tag="RD444_001227"
     CDS             11678..12553
                     /locus_tag="RD444_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7548610.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12705..13547)
                     /locus_tag="RD444_001228"
     CDS             complement(12705..13547)
                     /locus_tag="RD444_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7548611.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13639..15516)
                     /locus_tag="RD444_001229"
     CDS             complement(13639..15516)
                     /locus_tag="RD444_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDT7548612.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15513..16934)
                     /locus_tag="RD444_001230"
     CDS             complement(15513..16934)
                     /locus_tag="RD444_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDT7548613.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16937..20326)
                     /locus_tag="RD444_001231"
     CDS             complement(16937..20326)
                     /locus_tag="RD444_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDT7548614.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDCVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20583..21974
                     /locus_tag="RD444_001232"
     CDS             20583..21974
                     /locus_tag="RD444_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548615.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22047..22694)
                     /locus_tag="RD444_001233"
     CDS             complement(22047..22694)
                     /locus_tag="RD444_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDT7548616.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22702..23388)
                     /locus_tag="RD444_001234"
     CDS             complement(22702..23388)
                     /locus_tag="RD444_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7548617.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23394..24467)
                     /locus_tag="RD444_001235"
     CDS             complement(23394..24467)
                     /locus_tag="RD444_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548618.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24689..25363
                     /locus_tag="RD444_001236"
     CDS             24689..25363
                     /locus_tag="RD444_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7548619.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQPPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25368..26708
                     /locus_tag="RD444_001237"
     CDS             25368..26708
                     /locus_tag="RD444_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7548620.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26857..27843)
                     /locus_tag="RD444_001238"
     CDS             complement(26857..27843)
                     /locus_tag="RD444_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDT7548621.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28567..29598
                     /locus_tag="RD444_001239"
     CDS             28567..29598
                     /locus_tag="RD444_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7548622.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29707..31239
                     /locus_tag="RD444_001240"
     CDS             29707..31239
                     /locus_tag="RD444_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDT7548623.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIISLSPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31378..32190
                     /locus_tag="RD444_001241"
     CDS             31378..32190
                     /locus_tag="RD444_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDT7548624.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            32180..32665
                     /locus_tag="RD444_001242"
     CDS             32180..32665
                     /locus_tag="RD444_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7548625.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32688..33233
                     /locus_tag="RD444_001243"
     CDS             32688..33233
                     /locus_tag="RD444_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDT7548626.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33306..34949)
                     /locus_tag="RD444_001244"
     CDS             complement(33306..34949)
                     /locus_tag="RD444_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDT7548627.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35078..37306)
                     /locus_tag="RD444_001245"
     CDS             complement(35078..37306)
                     /locus_tag="RD444_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7548628.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37339..37818)
                     /locus_tag="RD444_001246"
     CDS             complement(37339..37818)
                     /locus_tag="RD444_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548629.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11086 a         7204 c         6913 g        12815 t
ORIGIN      
        1 tttaaaaaat gataaacctc tctcgaacgg ttttttatca aaaatcgtca tttttccatc
       61 aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt
      121 ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat
      181 atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga
      241 taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca
      301 ttttcggacc gattaatttt aaccattctg tttatttgct tacctattag atatcaataa
      361 aaaactgctt aacagctatt tcagctgtta agcagtttta ggatttttag ttcatattca
      421 ttgtttctta taatacagtg ttcaatactt ctcttaaagc acttgttgat ttgctgaaca
      481 ttgctttttc ttcttcgttg attgataatt caacaacttc acgaacacca ttttcgtcaa
      541 cgattgatgg cacacctgta aagatatctt tttcgtcgta ttcaccagtt aaataagcag
      601 atacaggcaa tacagcttgt tcgttattca agatagcttt tacgatacgt gcagtactca
      661 taccaatacc atagtaagtt gcttttttac gatcaatgat ctcataagca gcattacgga
      721 ctttatcagc aatcacatct aattcatctt gggcaatacg atggtctttt tctacaattt
      781 caaacactgg tttaccaccg acagttgtat gtgaccatgc agcaacttct gaatcaccat
      841 gttcacctaa gatatagccg tggacgctgc gtgggtcaac ttttaatttc aatgcgattt
      901 ctttacggaa acgagttgta tccaatgttg ttccagtacc gataacacgt gatgttggca
      961 gaccagattc gttccaagcg atatatgtca aaatatctac tgggtttgaa gcaacaacga
     1021 taataccgtc gaatcctgat cccatgattt cttttacgat ttggcgcatg attgacgcat
     1081 ttgttttaac taaatctaaa cgagattgac caggtttttg gttgatacca gctgtgataa
     1141 caacgatatc agcatcttta cattcttcat agccacctga ccatacagcc acattttcat
     1201 cgccccaagc cataccatct aaaaggtcca atgcttcccc ttctgctttt tcttgattca
     1261 catcgatcaa tactaattca ttagagattc cttggttgat cattgcataa gcgatacttg
     1321 ttccaacaaa acctgtaccg acgattacta ctttacgact tgtttttttc atgaataaac
     1381 agctcctttt cataactatt tgttactttc atgaaaagta tagtcttgtt tattcatttt
     1441 atcaacatgt taattataat agttgttatt ataatttttg atggaaacga attcctgaat
     1501 acgcataaca gataaatgac aaatgtttac acctatatcc cctgataata caaccaaaag
     1561 tttagaactg aaaataaatc caaaaaaatt cgctgcaacg atataataag acagcaatcg
     1621 cacctaaaac aattttcctt catatgcttt agtgaaagat tttcttctta atgtttattt
     1681 ctccattcat tgggagtaca tgttgaatac ttcttgaata atttgaataa agaagtcgtt
     1741 tgtttatatc cgatacgatt agcgatttct tccaacgaaa aactcgtttc ttgaatcagt
     1801 tccaacgcaa agtcgaattt tttctttaga accagttctt gaaaggaagc atcaaaatac
     1861 attttcaatt tattactaag atagaccgga ttgtagccaa aatgagctgc tgtttctttt
     1921 aacgtacaag aagtaaaatg cttatcgata taatctaata ctctaagcat ctcattttct
     1981 gcgcatccat ctgctgatga aaagctttgt tgttctaata aatgagtcaa ttcgatcagt
     2041 aatagtgatg tatagagatt taacgaacgg atcttatgac tgtatgtatc aaaataaatc
     2101 aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg
     2161 aattgtgtat gttccccttt ttcttttgat gcatttatca aaaaagaaga aagtagatta
     2221 tttgtgcttg ccaaatgatt caatatctct gtagataagg tttcttcttt taataagatg
     2281 ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt gagtagcttc tttatcaatg
     2341 atcaccacgt cactttcttc catctctatc ctttctccat taataatttg taaacatgta
     2401 ccgctataca tataattgat ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat
     2461 cgacggtgtt tactgatatg aatatttccc tgttgaaata gatcccctcg gtggattttt
     2521 ggcggtgtct gaagatggta agctaagttt aaatcattga tattcattcc ttgttcacgc
     2581 tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa
     2641 ttgactccct cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat
     2701 tatatgcaaa gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct
     2761 gtgattccgc tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa
     2821 aatgaaagga acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct
     2881 cttattttga cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat
     2941 gccacgcgtt ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact
     3001 atttttgttt tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc
     3061 atcggattag tgattgcatc gatttatggt atcctccccc tcttctcaca aagttacagt
     3121 attgtcttag cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca
     3181 gtcagcatga ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa
     3241 agtgcatttc aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag
     3301 ttcggttggc aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt
     3361 gttttgtttg ttcctacacc tgaaacaact gaacttcaat cacatggcac agccccaaca
     3421 agacaagtaa tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc
     3481 atcgtggtat ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta
     3541 ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc
     3601 ggagtttgtt ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta
     3661 ctactgatgg gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg
     3721 tttggtgcga tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa
     3781 gtaagtatcc aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc
     3841 gctaatttag gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt
     3901 gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt
     3961 attagccttc tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa
     4021 ataaaagaac aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa
     4081 aaacataaag atagatggct ttggtatcct ggagactttg agatccgcca tggactgcta
     4141 caaaatttcc aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat
     4201 tgccatcgga acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa
     4261 gtgacgatcc aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa
     4321 tggctgactt gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg
     4381 ctaccagcga tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt
     4441 aattttattg aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat
     4501 ccaaatcaaa tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc
     4561 ggaggaatct tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg
     4621 gatgaccata tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat
     4681 atcgattatt gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc
     4741 gcttttcgct atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt
     4801 catgaatttt tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat
     4861 cagatctggt cagtatcaga agaaaccttc aagttatgta gtgatttgtt ttttatcgac
     4921 ggtatcaaac gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat
     4981 caatatcttt tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa
     5041 aatgaaatca agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt
     5101 atcgaaaacc actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag
     5161 atgcaatcat taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg
     5221 agagcaaaag attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct
     5281 gcagaacaag tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta
     5341 gctgaaacat atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa
     5401 ctattgaaac aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat
     5461 tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc
     5521 gattttgcta acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact
     5581 atcacacaga ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc
     5641 ggacagacga atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat
     5701 gatgcggtaa ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt
     5761 atgtatggag atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat
     5821 cttttaggac gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg
     5881 atacaaccta atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga
     5941 aagatttcgc ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga
     6001 acagtgaaaa tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca
     6061 agctattcaa aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta
     6121 aatcttcttc tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt
     6181 tagaaacaat gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa
     6241 agaaagcgat gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta
     6301 caagtcctgg tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc
     6361 ctaatactgg aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac
     6421 ctaataccgc ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc
     6481 agaagaaatg tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa
     6541 acatggatag tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg
     6601 catatggaaa aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag
     6661 caatcccttg gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga
     6721 taacaaaccc agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc
     6781 cattgcttag ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata
     6841 cgatcgacat caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag
     6901 gaatctttat gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac
     6961 tagaagcata accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt
     7021 tcttcataaa cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc
     7081 cgcctaataa gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg
     7141 ttagacaaga aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga
     7201 atctagcaag taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac
     7261 cgtattgttg caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat
     7321 gcagagctgt ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac
     7381 cgccgatcag tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa
     7441 atttcttctt ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct
     7501 gactgacgat tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat
     7561 catttttatt cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg
     7621 atcgattgca aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa
     7681 tgctcaatga agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt
     7741 acttcaaact cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct
     7801 accataaaac ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc
     7861 tccttttctt atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag
     7921 attttgttta ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt
     7981 atcgatagca gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc
     8041 cacaggttgt tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg
     8101 agcatgaggt aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg
     8161 ctcgatcata gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc
     8221 ggcaaatgtg attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc
     8281 tgttaataat tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat
     8341 tttacgattt aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata
     8401 aggcagtagc gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca
     8461 atctctttgt acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag
     8521 aacatagaga gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc
     8581 gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat
     8641 catcgggaat aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg
     8701 acagacgatc acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc
     8761 tccgccaaat agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg
     8821 tctttctaac ggaagtaatg cttttcttgt catttcgtag atggaagcat attgatgctt
     8881 aatttgatcg atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg
     8941 gaccaagtga taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat
     9001 caggaattcc atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg
     9061 ctcctggaca tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga
     9121 attttgatgt tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc
     9181 aagagaagcc aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat
     9241 ggttgtcaaa ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc
     9301 tgatagtgaa atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag
     9361 ggttgcggtt aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt
     9421 gcgatttttt gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt
     9481 cctagtatac gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc
     9541 tgacaggact gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga
     9601 cgttgcagtc gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac
     9661 agcttcaaat cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt
     9721 tatacggata cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc
     9781 gcttgtattt acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt
     9841 caggagataa ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat
     9901 ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac
     9961 tgtaccgaac gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct
    10021 gcttttatga attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt
    10081 cttcaaattt ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg
    10141 gctcattagg ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat
    10201 tttctgtttc accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag
    10261 aattagttcc tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc
    10321 gtttgtgttt ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt
    10381 ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt
    10441 catatttttc cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt
    10501 aagattgttt cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc
    10561 ccttcgtaga cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc
    10621 ccttgttcat ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct
    10681 tcatgatcag gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc
    10741 acgaacatcc ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa
    10801 ctggatttta tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc
    10861 attcgtcctt tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa
    10921 taagaaagaa cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta
    10981 gaaaattcat gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact
    11041 tctaaaatat cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa
    11101 tcgaaccgtc tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat
    11161 aactcttctg cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca
    11221 tgttcgagtg ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact
    11281 aattggatct caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg
    11341 aatgttttcc acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta
    11401 agttttcttg aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg
    11461 attgtaagca tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc
    11521 agaaagtatt tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc
    11581 ggtttggtga aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca
    11641 agtgtttatc agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac
    11701 aaaacaagag ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat
    11761 cgaagctgca gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc
    11821 agctatcatt cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga
    11881 gctggggcgg gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt
    11941 tcctttttca agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat
    12001 ccagtcaata aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa
    12061 agtattgaac aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc
    12121 aaggaaattc cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata
    12181 cagtgattct tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag
    12241 ctacagcggt cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc
    12301 ccctaccttg ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg
    12361 tcttgttata tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat
    12421 tgccttcgat tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga
    12481 gaatatccgc aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata
    12541 tcggaaaact taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc
    12601 tctttgatat catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt
    12661 ttacggaacg attcgaatag atatattata gaaatgagga atagctaact tctgtttctt
    12721 attttgaacg attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct
    12781 aacaatccat acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact
    12841 tcttttgcat gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata
    12901 taaggtgtac aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt
    12961 gtatcagtcg gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg
    13021 atttcgatat agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct
    13081 tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa
    13141 gacgttcctt ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa
    13201 attggcaaat ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa
    13261 taggttctat cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt
    13321 ccaccttctt ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg
    13381 acttcttcat tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga
    13441 taattattca gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc
    13501 aagaaaagca tcagcagatc tagaagtttc cttttctttg gtttcattcg tgttctcctt
    13561 gtgatgactg tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt
    13621 ccaattcttg caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc
    13681 aatcagtaga aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga
    13741 aggaagtgta cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt
    13801 tacataagat tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc
    13861 tggcgctttc gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa
    13921 accgttactt gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc
    13981 ttttgtggat gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac
    14041 gaattctgct ccagcaagtg tttgatctga tgtaacgtca ccatctactt taacaaatcg
    14101 tttgccccca gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc
    14161 gacatttgct tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata
    14221 gtaaacaaat ttcagcgtac caccaggagt gagtgaagga atataattcg agtcaactga
    14281 aaccgtgaat ccgtttgttt gttcagtgac agaataattt gttggatcga tttctttatc
    14341 cccatcatac aagacatacg catacgttcc tgaagtttca tgatcaaatg tcaaagcggc
    14401 gtcatgagta tcaatgagtt tgaatgtcgt gtacttgttt tgtgttcctt ctttatcagc
    14461 gatacctaaa ggaatattga cagagatctc atattggatt ttttctccga ttgcgacatc
    14521 ttttccattc aattgaggtg ttgttttatc aactttggac gtatcatttt tgattgtctt
    14581 ttctacagga gtttgactat ttgccaagat ctcaaacggc gtgtttgttt gattatcaat
    14641 catttccgca ttatcaggag cttttacttc ttctaaatta tattttccaa cctctaaatg
    14701 attgacgatc aactgacctt tttcaatcgt tgcctcggca aagtcattgt tgccgatgtc
    14761 ataagaatga ccagtaatga aatgtttggc tttggtttga tccgttgtcc aagtgtacaa
    14821 tccatctgtg acactttgga tgtatttttt gacgcttggt cttccttctt ctttagaaat
    14881 aataaattct gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa
    14941 cgttccatca ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt
    15001 cccgtattta taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac
    15061 catgtttgcg gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc
    15121 tttcccattt tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc
    15181 tgaagcaact ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga
    15241 cgctccttta tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat
    15301 atcggctaat ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa
    15361 aggatcgggt aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc
    15421 tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac
    15481 tcctaacata acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg
    15541 ataaacaacc agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt
    15601 ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc
    15661 aatatactgt ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct
    15721 gtttcactta aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt
    15781 ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat
    15841 tcgttctttg tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt
    15901 ttaataagga atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca
    15961 aattgcttac tccctgtata tacagagact ttctttgtac tgataccaaa accaaaatcc
    16021 agtgttccgt tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga
    16081 taagaaatgg tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca
    16141 tcaaatccgt gaggtttttt gtgtatgaca aagccttctg tcagcttttc tccattcgat
    16201 gaaatcgtta aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg
    16261 aacttttgat agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt
    16321 tcaccaatcg taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg
    16381 tagtcgtttt cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt
    16441 ccatgtacag gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtgct
    16501 ttggattcaa tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa
    16561 aaagaagcga taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacgta
    16621 ccggaagacg caccgttttt tgcgatctct gtttgtgctt cttctaccgt tttgcccttt
    16681 tcccgtagat ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat
    16741 cctcgatacg tttgtaataa cgctttttct tgtccgtcat ttggatgatt tttcggcagt
    16801 tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct
    16861 gttgctgcct gagtaaaagc tggtttaaat agtaacaaga agagacacat actaagccaa
    16921 ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga taccaaatag
    16981 aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt cctcccgttt
    17041 caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg tttttggctc
    17101 ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca tcttcgtgta
    17161 taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa gtttcggtca
    17221 gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct tgtccatctt
    17281 ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct tttaatgtct
    17341 gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag
    17401 tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg atttttccat
    17461 atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca ggaccagctg
    17521 tttcaacgac tcggtattta ccaggtgtga gtgaatcaaa actggcaaga cctttttcat
    17581 tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc tgtagggaaa
    17641 atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac ttacgtactg
    17701 ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca tcggaagtag
    17761 tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt tcccaagtag
    17821 tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta taggtcattt
    17881 ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga tctgttagta
    17941 aagtatcaat agaatctcct gttaatttga agacagcacc gatgagattc ttttcacctg
    18001 tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta tttttccatg
    18061 tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg attgtttggt
    18121 aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct tgataacttt
    18181 ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgaattt gtagtatctt
    18241 tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt gtcgtatgtt
    18301 cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg tcaaattctt
    18361 cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt ataccgaatt
    18421 cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc atttgatacc
    18481 agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa tggatttgga
    18541 tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct tctatggaaa
    18601 tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt gtaccaggct
    18661 gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca gttgctgaga
    18721 tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca gctgattcat
    18781 agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac tctacttctg
    18841 cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca tgtatttcga
    18901 ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta ctgtcgattc
    18961 ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga gaagtatttc
    19021 ccggccgatc ttgcgtatta gaaaactgag ttccgtaata attgctgctt gattgtgcgt
    19081 gtacccgctg tactttatag gaaaatgttg gtacacaatc cgtcagcaaa acgatcactt
    19141 ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa
    19201 aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttgatttttc actgaatcaa
    19261 atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag tagccgacat
    19321 atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca aaacgatcga
    19381 caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac cagtctacga
    19441 ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca ttcaaataaa
    19501 catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca tagtctgctt
    19561 gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga tctccattcc
    19621 aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga tcaagaacat
    19681 ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca tctgttgtat
    19741 attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat aatgtaggca
    19801 atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct atttcgtctg
    19861 gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata atgtcatatt
    19921 ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga tcgattggaa
    19981 aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt ttcccgctat
    20041 ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag taattttcga
    20101 ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt agcttgatgt
    20161 tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg
    20221 ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat aaactcaata
    20281 ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt cccctccttt
    20341 ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata
    20401 attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa
    20461 ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt
    20521 gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaatga aactatagaa
    20581 agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac
    20641 ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa
    20701 tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac
    20761 atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca
    20821 gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa
    20881 aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag
    20941 aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg
    21001 agctccagtt gcgtatcttt gtcacctttt tctttttaag ttactacgga agacgcacgt
    21061 tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga
    21121 tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg
    21181 tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg
    21241 tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat
    21301 tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat
    21361 tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt
    21421 cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc
    21481 aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa
    21541 cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag
    21601 aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta tctagctata
    21661 tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac
    21721 tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata
    21781 ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga
    21841 aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg
    21901 ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct
    21961 tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca
    22021 ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta
    22081 tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa
    22141 gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga
    22201 atggagaaat gattgattct taataattcc agccattttt cgttcattgg agaatcagcc
    22261 gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt
    22321 tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc
    22381 aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga
    22441 gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa
    22501 gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc
    22561 tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa
    22621 ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag
    22681 cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc
    22741 ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat
    22801 agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg
    22861 atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa
    22921 agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt
    22981 tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt
    23041 ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa
    23101 attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc
    23161 tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa
    23221 tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac
    23281 gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc
    23341 agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct
    23401 cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc
    23461 attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa
    23521 ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg
    23581 cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg
    23641 atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg
    23701 aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc
    23761 atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt
    23821 agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg
    23881 atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta
    23941 tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca
    24001 atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa
    24061 tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg
    24121 agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt
    24181 tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat
    24241 ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa
    24301 acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc
    24361 gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag
    24421 ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc
    24481 ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca
    24541 atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa
    24601 accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact
    24661 aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa
    24721 agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta
    24781 tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga
    24841 tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat
    24901 acagcctcca gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt
    24961 tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg
    25021 cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa
    25081 ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat
    25141 acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac
    25201 aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat
    25261 tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat
    25321 caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata
    25381 ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct
    25441 tttttctttt gggattcatt actttacaga tattaaacca atctgcatac agagaaacag
    25501 atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat
    25561 atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg
    25621 aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta
    25681 aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat
    25741 taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg
    25801 gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg
    25861 ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc
    25921 gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt
    25981 acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg
    26041 ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac
    26101 atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat
    26161 tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg
    26221 attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac
    26281 ttgttaaacc atttcaagag atggccacca tagatggaaa agagtttatt ctccataacg
    26341 atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc
    26401 tggataatgc attaaaatat acgaatccag aagataagat cattttggat tcatctgtta
    26461 accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc
    26521 aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg
    26581 gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt
    26641 ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta
    26701 aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gcactttttt
    26761 gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa
    26821 gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc
    26881 aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac
    26941 tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg
    27001 tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaagtt
    27061 ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc
    27121 acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac
    27181 ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt
    27241 atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc
    27301 agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt
    27361 attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa
    27421 tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt
    27481 tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc
    27541 tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa
    27601 agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt
    27661 ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc
    27721 atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat
    27781 tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc
    27841 catgtaaatt cctcctagca aatagattta tgattggtat attgttactt ataaatttat
    27901 tctataggag acgattgttt tctacaaata tttagattgg aatttgatta aaacaattta
    27961 attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat gtcatttgtt
    28021 tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat ggtttaattt
    28081 atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc aatataattg
    28141 ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt tctaatactt
    28201 ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa tggagagcca
    28261 atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc ctcctgccat
    28321 tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa aaagaggata
    28381 aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa tgtatcttct
    28441 tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat caagacaaac
    28501 tgtctatctt catttgatga aatgcggtaa aatatagaaa atgaacacta ttggagggaa
    28561 tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca acgtacacac
    28621 ttgctccctt ccgtctttca acagttttta gaagaaaatc gttcttccct ttcattaggc
    28681 agtcgctatg aatacgcaaa agattttcat ctgtttactg attatttgag aaaagtattt
    28741 tctgaagaag tggatgatca gctgattatc catttagaaa aacaagatta tcagaatttc
    28801 ttagctaaaa tctatcgaca tactcgttcg ttccaaacta cagcgaacca agaaacggag
    28861 caattgtttg aaaatagcta ttacgggatt tcaaggaagc aatattcatt aaatcatttt
    28921 ttacgatttt tgtatcaaaa aggactgatt gaagaagaga tttctctttt gggtacaagt
    28981 ttaccggaaa caaccaaagc agctccgcga tatatcgatg cagaactttt caaagatttt
    29041 gagcatttgt ttatcccgat tgaaggcttt caaacaagca gagaagctag ctttgaagaa
    29101 aagaatagac cgaggaacct tgcaattact gccattcttc tgtattgcgg attaaagatc
    29161 catgaagtag tagaacttaa acgtaaagat gtatctttga ccaagcagat tcttaatctg
    29221 aatagaaaag aaaacagact gaataaagta ccaataccaa aagaagcgat gccatttctg
    29281 gagaactatt ttaagttaac agcacaaaat acggacgaaa attcttttgt ttttcgttcc
    29341 tcacatgatc agcaaatttc tcctcggact attcgccaaa ttttgagcaa aattacagta
    29401 tataaaaacg tatcgccaga actatgcaga aaaacatatc gttattatgc agaactatat
    29461 ttagatgatg ctgacgcggt aaattattta tgtggtaatc gatacctctc atcccattct
    29521 tttcaagata tctgggaaaa gatggttgat ttttcttatc acgaattagc ggcattcgtt
    29581 cgggtttctt ccatataaaa caaatgtatg atcttttttt cacaataaac tatgttatta
    29641 tataaaggat tatgttacga ttatcttgaa tttgtatatt acttaacaaa agataatgga
    29701 gtgtgaatga tggagaccat aacgtattcc cctttgaact tatttacgaa ttttcaagaa
    29761 gctgcagcta acttttccga gaccccgatt atctttgatg agccactttc agctttccca
    29821 gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa acgggcttat
    29881 caattggctc atcttggagt gaaatccgga gacaaagtta ttatttataa gagttccgct
    29941 tttgacacat accttctagc tgtttctgtg tcttaccttg gtgcagtgcc tgtcatgact
    30001 tcttatcatc taccaacaac aacaatggaa gtatttatcg atcgtttaga agaccctttc
    30061 attttatttg atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg aacaaaatca
    30121 aaacagattc ctattcttta tttaatggaa caacctgctt tgtctgtttc tcaatcattt
    30181 ttagacaaaa atgaaatttc ttatatgacc catacttctg gtacaactgg tatccctaaa
    30241 cttatttgtc attctgctca ttctatggga tggcggacga aatggcaaaa aacagttttc
    30301 acaaaaattt cggaaaagaa attaatcgct tttcacatct ctccagtgca ttccagattc
    30361 aatattggta tctcttcttt gatgtccatg ggatttccta tgatgccttt agcaaacgca
    30421 caaagttcaa aagttgttca tatgttggaa gctcaccggc caattgcact agagacccat
    30481 ccgaataact ttgttcagtg gcgttttact gctaaggagc atccagaagc tttttcgggt
    30541 atccgatact atcactccac ttttgatgct ataaacaacc agacaatgaa gacatttttg
    30601 cgaacgtcga tggcacagga tgcggtattt cttcaagtat atggccaaag tgaatgcgga
    30661 ccaatgattc ttaaggctca tacactagag agtttgaaaa caagtgatgc aagagatatg
    30721 ggcgttggac tggaagacat gacaagcgct cggattacag attcagttgg gaatgtccta
    30781 cctgaaaaca cagatggtca tatacaatta ctgtctaaag gacgggcttt gacttattac
    30841 aaggaagatg cacggttcca ggaaaatgtt tatggcgatt ggtgggatag cggagattac
    30901 ggttttatgg ataagcatgg ccacctattt ttgaaagatc ggcaagtgga tttgatcgaa
    30961 acgatcaata gtacattagc tattgaagac tttttattag attcgttaga ttttttggca
    31021 gaagtagtga ttgtaaggga taaaaatcat tctccacaac cgattatttc tttatctcct
    31081 gataaagaaa tggattggaa tcgatggtgg gagcaagtcc atgaacttcc tcgcttgaac
    31141 gtccctatca tccgtgattt tgatgcaatc ccgcgaacag ctacaatgaa agtgcaacgg
    31201 cttcaaatcg agaaagaatt aaaatcacaa aagtcctaac tatttaagga aagaaagatc
    31261 cattttggtc tttcttttct ttttgttttt ttaaattgga tgagataaaa atatgccaat
    31321 tggctgtttt tcttttttga aaatttcgtt atactcttat tcatggagga aaatgttatg
    31381 aaaagaatct tgacaattgg cggttctgat ccatttgctg gtggaggaat ccaaacagat
    31441 ttaaaaactt ttgagaattt tggtctattc ggactaagcg ccttgacttc tataggggca
    31501 ctagatatta atgagcgatt tatgctggaa tctatatctt tggaattgct taaacgacag
    31561 ttagtttcga ttgataaaat gacttcatta gatggtatca aaattgggtt attgaattca
    31621 agtgaagcaa tcatggttgt tcgagacttc ataaaaacaa aacaaaatat cccaatcatt
    31681 ttagaccctg ttttggcatt caaagaaact gaagctgtga tcaatcaatt gtatatttcg
    31741 cagctcatcg aagaactttt tccacttgtt acgatgatta cgcctaattt ggcagaagct
    31801 gctttacttt ctggtcaagc tcttccctct tcgttaacag aaatgatcca tctcgctaaa
    31861 gaactcactg cttcaggaac aaaaaacatt gtgatcaaag gcggcacaag gatggctgga
    31921 gatgaagctg tggatcttct ctattcaatt gatcattatg aggtattttc tcataaaaaa
    31981 atcgacacta caacagtaaa cggggccggt tgtgcctttt cttctgcaat aacagcgaat
    32041 cttgtgttag gggcagattt taccaaagca gtagcagaaa gtaaaaaatt tgttgcccat
    32101 tgtatttcta atggcgtgct gatgaacgat cataccggaa gtgtctggtc tggcggtatt
    32161 caaaaaggag gaatgagaaa tgaaaactaa gcaattaacg atctatgcta tgctgattgc
    32221 gttgacagtt gccctttcat taacaatttt gatccctgta ccggctacga atggatttgt
    32281 gacattatgc gaggccggga tctataccgc tgcttttctt tatggtccaa cgggtggatt
    32341 agttgttggt gcttccagtg gtctattgat cgatttgatt tcaggatatc cgcagtgggc
    32401 agttttttca ttagtgatcc acgggttaca aggatatgtt gctggttctt tcagcaaatc
    32461 ttctactcgt atgtggggat tagggctttt attaggaagc ttagttatga tcatcggcta
    32521 tggtatcgcc ggttggattt tatatgattg gccggctgga gtggcttcta taccaggaaa
    32581 catcttccaa aatctgattg gaatgggact tgcctttcct ataactgcaa gtcttcaacg
    32641 tattcaagca cggaagcaat attaattgaa aaagaaggtt ttagaaaatg aagttgtcag
    32701 aaaaagaatt aaaagaccaa ttgactgaaa tagttaatga catattggct gaggcacact
    32761 tgaaaaaagg tgatcttttt gttttaggat gcagcacaag cgaagtcgtt ggaggacata
    32821 ttggaaaaaa ttccagtgcg gaagttggtc aatggatcat ccgtacgtta aaagagttgt
    32881 tggatcctaa agaaattgcg ctagctgtcc aaggttgtga acatttgaac cgagcattag
    32941 tagtagaacg aacagtggca gaagcaaaaa atttcgaaat tgtctctgtt gttcctgcac
    33001 tacatgctgg aggtgcttgt tctgttgctg cttttgacca gttcaatgat cctgtcgaag
    33061 tggaacacgt agttggacaa gccggtatcg atatcggtga cacatcaatc ggtatgcata
    33121 tcaagcatgt ccaagtacct gtcagacctc gtttgaaaac ccttggacaa gctcacgtga
    33181 cagcccttcg ctctcgtcct aaatatatcg gtggtcctag agcgaattat tgaaatgagt
    33241 aattgacaga gcaatctgaa aaacttcctc gcaaagtcca actaatgaac tttaacgagg
    33301 aagttttact tgtttgtttt atataaccga gcttcataag gtgctagtat gcctttagct
    33361 tcaaattctc cgctagagga atttgacaag agtaatgtcc aggactgatc tgcttctttt
    33421 ggaagttcat agtcagcaag tgattctgtt aagtttacca taataagata ttgttcatct
    33481 tctaaatatc tttcatagac ataaagttgt ggatgttcat gaagatattc tttataagaa
    33541 ccgtatatca gacctttatt ggtttgtcta atgcggatca tgttcttata gaattgcagg
    33601 accgactctg gttcatattc ttgttgttct acattgatcg ttttggtatt tggattagtg
    33661 atcagccaag gttcatgtgt agaaaaacca gcatattgtt ccgaagtcca ttgcatgggt
    33721 gtcctgctgt tgtctctcgt tgtttcacga atgatatcca atgcttcttc tcttgtctta
    33781 ccttccgcaa gcagcctttt ataaagtctt ttcgaatcta ctgcatcaac ttgatcgatc
    33841 gattcaaagg gcatattcgt cattccgatt tcctgtcctt gataaataaa tggcgtacct
    33901 tgcaaaaaga aatacatcat agcgattgct tttgcagaca tagcccaaaa atcgggacga
    33961 gtatctccaa acactgaaac ggcacgagga acatcatggt tttccatata taaagcgttc
    34021 catccgatac catccaatga agtttgccaa gcgcttaatg catgtttcaa tttaaggaca
    34081 tctaattgcc cttctttttc ttgctgccag atatggatat gatcaaattc gaatatcata
    34141 tcgaaataac cgtctttccc tacccattgt ggtccttctt ctgcagtgac accacttgct
    34201 tctccgacag tcatgatatc gtactcttca aaaacatgtt tcaaatcggt caaatgttct
    34261 tcgattcctg agacattttg aaagggtgag aaagggttct ctgtggcttt tactgaatat
    34321 tcatcttttt ttacatgaga aatcgcatct aaacgaaatc catctatccc tttatccaac
    34381 caccatcgga tcatattgaa tagttcttct ttcaatttct cgctttccca atttaagtct
    34441 ggttgttctt ttgcaaaaac atgcagataa tactgtccag tttcatgaga atactcccaa
    34501 gcagaaccgc cgaaaatcga tgtccaatta ttcggttctt tgccatctac cccgtcgacc
    34561 caaatgtaat aatcgcgata cgggctttct ttagattttt tcgattcttc aaaccattga
    34621 tgctgatccg aactatggtt gatgacaaga tccataatca ctttgatccc taattcatgt
    34681 gcttctttta gcaataaatc aaaatcttcc atcgttccga atctctcttc tatatcttga
    34741 tagtcactta cgtcataacc attatcgaca aatggagatt gatagatcgg attgatccag
    34801 ataaagccga taccaagatc tttcaaataa ttaagttttt cacggatccc attgatgtct
    34861 ccttgaccat cgccattgct atctttgaaa ctagcaggat agatttgata gcctactgct
    34921 tctttccacc atttttgttc ttgtatcata tcgtttcact cctttaacgg tccctcttga
    34981 ttataaccga ttctatttta gaagtatcct tgttaccggt aactagaaac gaaaagctca
    35041 acgaatctgc taagtgcgtt cgctgagctt tattttatca tagatagcgg gcaatcagtt
    35101 gaatcatttg cttgggaaat tcattcaagt cagtaatatc tacaaaacga ctttcaccgt
    35161 agatcgactt gatttcttct ttgtcttggc caatagctgc ggagacaaat aggattcctt
    35221 gacgttcata ttctttcaaa acagactgga tatcttcttt tgcttttttg cctgtataat
    35281 ccggcaacgc ttttggctga ccgtcactaa tattaagcaa cagtttggtt gttgcggatt
    35341 gtctgtttaa tttttctgct gctaaacgaa gtgcagcacc atcacgattg ttttgccttg
    35401 gtttcatagt gactaatttt tcatttaacc attgaaatcc gtcttcaaat tctttataag
    35461 aaaacaaaga agtcttttct cttgacgaac ggtctgctgt atctccgtaa atcaacaatg
    35521 ggatatcgac tttcttagaa aattcggcaa tggcaatcgc tgcttttttt gccgcttcga
    35581 tccgatcatc ccgtatcatt gacccagatt catcgatccg tacagctacc gctaaagaag
    35641 gttgctcatg tggcgggttc tttttgtcaa aatttctcaa atcaccatat gctactttgc
    35701 ttgcattgaa ccgctgacct tcgtattttc ctttttggta cgtctctgtt tgttcattat
    35761 ccagtaactc taaaatttgc cggctaagtg attctactat tggcattact tgcatgtgta
    35821 gctcacgtgc ttccggcgtt tttccttgga aagctggtcg gtgaacgatg agaccttctt
    35881 tttcatgaat cgtttccttc atgatttgtc gcgcttcttt attcagttgc ttcttgattt
    35941 ccttatcatg tgcttccatg tcagaagcag acatttcttg agaatcatct cctgtttgtt
    36001 gttctttttg attttttcct tcgatatcgc tactgcgttc tttttgcatt tgaggaggaa
    36061 tctcgttttt ttctattgtt tcttcttgtt tctcttctcc ctcatttggt tcttcagcag
    36121 acaacgattt cttttcatct aagcgcagaa tttcttcagc attttcagtg atgtttcgtt
    36181 catttgcttc ttcggcgcta atggttcttt cttttgtatg ctcagattga ggatgacccc
    36241 atttttcttt tctcaatatg tcctgaagtt ccagtagcaa tccactgtca gacaaagctt
    36301 gttcaagaat tgccaattca tctgaatcag tggataattt atacaaaact tttggataaa
    36361 tcgactggag taaatcttgt ccttgcttat aggtatttgc ccaataaaaa taagaacgca
    36421 ttcctgctac acctttgatg gcgttagctt ttgcagtttc atccaacaaa cggataatct
    36481 ctgccatctt taagagaagt tcttgttttt ggatcatagt tttcgccatc gcacgttctg
    36541 ccatgacttc taaggcagga agatccattt tttccgcatg ttgcatccga tctcttaaag
    36601 attcatttaa tgggcgattt ccttggtaat ttcgatttgt agtgatcaca atgacgcaat
    36661 ctggatggcg acgaatgatt cctgtcggta aattcaacat cccatttggt tcgagcgctg
    36721 agttaagtgc cacaagaaca gaggcatctc ggatgacagt cggctcttga atctctaata
    36781 aatagccttt ttcaatcgct cgaacgattt cagaagggta atagctgtaa tggattgcat
    36841 ctggataatc agcttcgatc ctttgaacta gctctgctaa tttttcttta gcagaaaaag
    36901 gatcaatgcc gtaatggttc tggatcagtg tcagtacagc ttcaagtgta tctgtttggt
    36961 aaatagcttc tagtaattct tggtcttgtt ccttattaga atctactaca ggtaataagg
    37021 ctccgaaaac atctgatttg tccatatctg caaaacaggt cacttttgta tagggaagtt
    37081 gcagatcagc agatagtgct ttggctaatt gtgtcttccc cgacccggca tctccttcca
    37141 aaagtacgtt actgattttc atttcaggat cttgccaatt ccctttgatt tcggaggcga
    37201 tccgccattc ttctgcactt gtccgatggc ttttcggttt cttccagatc atttgctttt
    37261 ctagccgtga aaaggttcgg ttttctgtta gtgagaaatt ctccatgctt ctcttcccct
    37321 tttgatcatc tctgtctatt aatatcctag ctcatctaaa agacgtttta aaatacggaa
    37381 tcgttcccca atcagttctc cgtctttttg tagtgtatcg taatacaaaa tgatgtcttc
    37441 atcgatcttt ggatttttgg tatctacttc taccgtcatt cggttccctt gtaatccaat
    37501 gatgtctacg actggatttt ctggatcata aaatttttca taacgataag catcttggaa
    37561 gtataaactt ccttgcgcag ctaagatcgc taaatctaaa atacgtgtga ttggtaattc
    37621 ttccgactgt cgtgaccatt tttctccagt atgtcgccat accttgcctg agatatcgac
    37681 ttttccacga tcattccact gagcaaggcc cagtgaaagc ccttttgcat ccatatttaa
    37741 tgcatttctt ccatcaactt tatcatagtc ttctacaacg attactggtt tatgttttaa
    37801 atttgttggt atttccatta ttttaactcc tcatcctttt atttttagta aataagtaaa
    37861 ttagtagttt actcatttac taaactatat gcgacattat agaggattga agtgaggatt
    37921 gcaaagaaaa agagcttcat tgatagaaat aaaaacacca tctcgttctt ttacacgctc
    37981 attagtaaca tgtagaaagt ccaagatgat gttggaat
//
LOCUS       DAPBAB010000019        37732 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPBAB010000019 DAPBAB010000000
VERSION     DAPBAB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.019"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(159..1469)
                     /locus_tag="RD444_001247"
     CDS             complement(159..1469)
                     /locus_tag="RD444_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDT7548630.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="RD444_001248"
     CDS             complement(1481..2899)
                     /locus_tag="RD444_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7548631.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="RD444_001249"
     CDS             complement(3139..4707)
                     /locus_tag="RD444_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDT7548632.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="RD444_001250"
     CDS             complement(4711..5121)
                     /locus_tag="RD444_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDT7548633.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="RD444_001251"
     CDS             complement(5118..6041)
                     /locus_tag="RD444_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDT7548634.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="RD444_001252"
     CDS             complement(6038..6916)
                     /locus_tag="RD444_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDT7548635.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="RD444_001253"
     CDS             complement(6903..8240)
                     /locus_tag="RD444_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDT7548636.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="RD444_001254"
     CDS             complement(8243..9346)
                     /locus_tag="RD444_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7548637.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="RD444_001255"
     CDS             complement(9420..10928)
                     /locus_tag="RD444_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7548638.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="RD444_001256"
     CDS             complement(10894..11586)
                     /locus_tag="RD444_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7548639.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="RD444_001257"
     CDS             complement(11608..12582)
                     /locus_tag="RD444_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7548640.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="RD444_001258"
     CDS             12882..13754
                     /locus_tag="RD444_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7548641.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="RD444_001259"
     CDS             complement(14212..16410)
                     /locus_tag="RD444_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548642.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="RD444_001260"
     CDS             16642..17634
                     /locus_tag="RD444_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7548643.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="RD444_001261"
     CDS             17694..19115
                     /locus_tag="RD444_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDT7548644.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="RD444_001262"
     CDS             19108..20607
                     /locus_tag="RD444_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDT7548645.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="RD444_001263"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="RD444_001263"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDT7548646.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="RD444_001264"
     CDS             22199..22882
                     /locus_tag="RD444_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDT7548647.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRPP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="RD444_001265"
     CDS             22884..23720
                     /locus_tag="RD444_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDT7548648.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="RD444_001266"
     CDS             23731..25485
                     /locus_tag="RD444_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7548649.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="RD444_001267"
     CDS             complement(25761..26102)
                     /locus_tag="RD444_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7548650.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="RD444_001268"
     CDS             complement(26170..28341)
                     /locus_tag="RD444_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDT7548651.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28510..29409
                     /locus_tag="RD444_001269"
     CDS             28510..29409
                     /locus_tag="RD444_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7548652.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="RD444_001270"
     CDS             complement(29480..30337)
                     /locus_tag="RD444_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7548653.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="RD444_001271"
     CDS             complement(30322..33024)
                     /locus_tag="RD444_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDT7548654.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="RD444_001272"
     CDS             complement(33037..34326)
                     /locus_tag="RD444_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDT7548655.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="RD444_001273"
     CDS             34476..35522
                     /locus_tag="RD444_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7548656.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     SYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="RD444_001274"
     CDS             35524..37677
                     /locus_tag="RD444_001274"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDT7548657.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11154 a         7776 c         6706 g        12096 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtccgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catagttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aa
//
LOCUS       DAPBAB010000020        36567 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPBAB010000020 DAPBAB010000000
VERSION     DAPBAB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.020"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="RD444_001275"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="RD444_001275"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDT7548658.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="RD444_001276"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="RD444_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDT7548659.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="RD444_001277"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="RD444_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDT7548660.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="RD444_001278"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="RD444_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDT7548661.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="RD444_001279"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="RD444_001279"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDT7548662.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="RD444_001280"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="RD444_001280"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDT7548663.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="RD444_001281"
     CDS             complement(8052..8522)
                     /locus_tag="RD444_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDT7548664.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="RD444_001282"
     CDS             complement(8568..8993)
                     /locus_tag="RD444_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDT7548665.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="RD444_001283"
     CDS             complement(9060..9329)
                     /locus_tag="RD444_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDT7548666.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="RD444_001284"
     CDS             complement(9340..10935)
                     /locus_tag="RD444_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7548667.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="RD444_001285"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="RD444_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDT7548668.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="RD444_001286"
     CDS             complement(14488..15048)
                     /locus_tag="RD444_001286"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDT7548669.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="RD444_001287"
     CDS             15401..16375
                     /locus_tag="RD444_001287"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDT7548670.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="RD444_001288"
     CDS             16545..17945
                     /locus_tag="RD444_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDT7548671.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="RD444_001289"
     CDS             complement(18023..18745)
                     /locus_tag="RD444_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7548672.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="RD444_001290"
     CDS             complement(18824..19330)
                     /locus_tag="RD444_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7548673.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="RD444_001291"
     CDS             19453..21252
                     /locus_tag="RD444_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDT7548674.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="RD444_001292"
     CDS             complement(21320..22894)
                     /locus_tag="RD444_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7548675.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="RD444_001293"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="RD444_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDT7548676.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="RD444_001294"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="RD444_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDT7548677.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="RD444_001295"
     CDS             complement(24671..25309)
                     /locus_tag="RD444_001295"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDT7548678.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="RD444_001296"
     CDS             complement(25310..25960)
                     /locus_tag="RD444_001296"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDT7548679.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="RD444_001297"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="RD444_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDT7548680.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="RD444_001298"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="RD444_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDT7548681.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="RD444_001299"
     CDS             complement(29103..29843)
                     /locus_tag="RD444_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDT7548682.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="RD444_001300"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="RD444_001300"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDT7548683.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="RD444_001301"
     CDS             complement(31214..32152)
                     /locus_tag="RD444_001301"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDT7548684.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="RD444_001302"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="RD444_001302"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDT7548685.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="RD444_001303"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="RD444_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDT7548686.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="RD444_001304"
     CDS             complement(35312..35617)
                     /locus_tag="RD444_001304"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDT7548687.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="RD444_001305"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="RD444_001305"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDT7548688.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DAPBAB010000021        35424 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPBAB010000021 DAPBAB010000000
VERSION     DAPBAB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.021"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            93..764
                     /locus_tag="RD444_001306"
     CDS             93..764
                     /locus_tag="RD444_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7548689.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            823..1263
                     /locus_tag="RD444_001307"
     CDS             823..1263
                     /locus_tag="RD444_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548690.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1275..1943
                     /locus_tag="RD444_001308"
     CDS             1275..1943
                     /locus_tag="RD444_001308"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDT7548691.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            2007..2630
                     /locus_tag="RD444_001309"
     CDS             2007..2630
                     /locus_tag="RD444_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDT7548692.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2686..3072
                     /locus_tag="RD444_001310"
     CDS             2686..3072
                     /locus_tag="RD444_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDT7548693.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3285..4676
                     /locus_tag="RD444_001311"
     CDS             3285..4676
                     /locus_tag="RD444_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDT7548694.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5287..5655
                     /locus_tag="RD444_001312"
     CDS             5287..5655
                     /locus_tag="RD444_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDT7548695.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5835..6776
                     /locus_tag="RD444_001313"
     CDS             5835..6776
                     /locus_tag="RD444_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDT7548696.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6845..7576
                     /locus_tag="RD444_001314"
     CDS             6845..7576
                     /locus_tag="RD444_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDT7548697.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7720..9900
                     /locus_tag="RD444_001315"
     CDS             7720..9900
                     /locus_tag="RD444_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDT7548698.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9920..10402
                     /locus_tag="RD444_001316"
     CDS             9920..10402
                     /locus_tag="RD444_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDT7548699.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10418..11179
                     /locus_tag="RD444_001317"
     CDS             10418..11179
                     /locus_tag="RD444_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDT7548700.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11310..12644
                     /locus_tag="RD444_001318"
     CDS             11310..12644
                     /locus_tag="RD444_001318"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDT7548701.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12951..13436
                     /locus_tag="RD444_001319"
     CDS             12951..13436
                     /locus_tag="RD444_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDT7548702.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13429..14112
                     /locus_tag="RD444_001320"
     CDS             13429..14112
                     /locus_tag="RD444_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDT7548703.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14238..16883
                     /gene="polA"
                     /locus_tag="RD444_001321"
     CDS             14238..16883
                     /gene="polA"
                     /locus_tag="RD444_001321"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDT7548704.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16928..17764
                     /gene="mutM"
                     /locus_tag="RD444_001322"
     CDS             16928..17764
                     /gene="mutM"
                     /locus_tag="RD444_001322"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDT7548705.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17728..18357
                     /locus_tag="RD444_001323"
     CDS             17728..18357
                     /locus_tag="RD444_001323"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDT7548706.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18409..18741
                     /locus_tag="RD444_001324"
     CDS             18409..18741
                     /locus_tag="RD444_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDT7548707.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            19053..19544
                     /gene="nrdR"
                     /locus_tag="RD444_001325"
     CDS             19053..19544
                     /gene="nrdR"
                     /locus_tag="RD444_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDT7548708.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19567..20940
                     /locus_tag="RD444_001326"
     CDS             19567..20940
                     /locus_tag="RD444_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDT7548709.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20940..21866
                     /gene="dnaI"
                     /locus_tag="RD444_001327"
     CDS             20940..21866
                     /gene="dnaI"
                     /locus_tag="RD444_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDT7548710.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21995..22435
                     /locus_tag="RD444_001328"
     CDS             21995..22435
                     /locus_tag="RD444_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDT7548711.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22579..23280
                     /locus_tag="RD444_001329"
     CDS             22579..23280
                     /locus_tag="RD444_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDT7548712.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23277..24398
                     /locus_tag="RD444_001330"
     CDS             23277..24398
                     /locus_tag="RD444_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDT7548713.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24413..25645
                     /gene="pepT"
                     /locus_tag="RD444_001331"
     CDS             24413..25645
                     /gene="pepT"
                     /locus_tag="RD444_001331"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDT7548714.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25777..26685
                     /locus_tag="RD444_001332"
     CDS             25777..26685
                     /locus_tag="RD444_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDT7548715.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26720..26992
                     /locus_tag="RD444_001333"
     CDS             26720..26992
                     /locus_tag="RD444_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDT7548716.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            27003..28049
                     /locus_tag="RD444_001334"
     CDS             27003..28049
                     /locus_tag="RD444_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDT7548717.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28300..30909
                     /gene="clpB"
                     /locus_tag="RD444_001335"
     CDS             28300..30909
                     /gene="clpB"
                     /locus_tag="RD444_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDT7548718.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(31060..31746)
                     /locus_tag="RD444_001336"
     CDS             complement(31060..31746)
                     /locus_tag="RD444_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548719.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31724..32218)
                     /locus_tag="RD444_001337"
     CDS             complement(31724..32218)
                     /locus_tag="RD444_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDT7548720.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32238..33458)
                     /locus_tag="RD444_001338"
     CDS             complement(32238..33458)
                     /locus_tag="RD444_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7548721.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33605..>35424
                     /gene="lepA"
                     /locus_tag="RD444_001339"
     CDS             33605..>35424
                     /gene="lepA"
                     /locus_tag="RD444_001339"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDT7548722.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDE"
BASE COUNT        11864 a         6244 c         7607 g         9709 t
ORIGIN      
        1 aaatagtaaa tttttatgaa attctactct aagcatcaga ctttcttttc aaaatatgct
       61 attttaaaaa tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata
      121 ctctgattga gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc
      181 atttttcacc agagttccat ctagaggggc taacattagg agaaatctat caagcagaag
      241 ggctgtcggc tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata
      301 tcgagctttc tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa
      361 tgatcgatac aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc
      421 gttatcaaat agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa
      481 aaagcttttt aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga
      541 aaaaatatcc aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc
      601 atcgttttat acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca
      661 catttcgagt agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa
      721 atcggaatgt ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg
      781 atttttttgt cataataaat attagatgat ggaggagata atatgatgca aacatggtta
      841 gaaaggctaa tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa
      901 tttgatcagt tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt
      961 tggttgccct atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt
     1021 cgtttcctat caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat
     1081 cttgatagag tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat
     1141 cgaaaaagtt atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa
     1201 ggaaaaggac gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt
     1261 taagggagaa aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg
     1321 aaaattactg gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag
     1381 cagttgccgg tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg
     1441 ttttgaaacg tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc
     1501 ttcctataat aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa
     1561 acaaagataa gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat
     1621 atacggaagt tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg
     1681 atccgcattt gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct
     1741 tatggcaaga tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc
     1801 atggtaatag catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg
     1861 atacgatcga tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa
     1921 ttctggacaa aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag
     1981 aagcaaagga atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat
     2041 tgtttgatac gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta
     2101 tcagtatgga caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga
     2161 tcaatgaatc aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag
     2221 aaagtacgga agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag
     2281 acaacggcgg tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt
     2341 taaaagaaca tcgattggca catctgattg aggaagtagt agggatcgat caggactttc
     2401 ctcgtaaacc tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa
     2461 cagacacgat gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag
     2521 tcgctacttg tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg
     2581 taggaaatct gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt
     2641 caaaggagat atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga
     2701 caaaaagaag ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa
     2761 acagatttga aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa
     2821 catagcatcg taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct
     2881 ggaagtgtga gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc
     2941 acacaagaag aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct
     3001 tatgaggtga cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa
     3061 aaacaagaat agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt
     3121 ttttattact aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa
     3181 aagtcactga aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta
     3241 cttccttcga tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca
     3301 cagttttagt ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc
     3361 gccatacaaa attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg
     3421 gtctgcttca attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga
     3481 tcgggattgt aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt
     3541 ttatttcgat cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta
     3601 gtgcgactgt attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg
     3661 gttcaacaat gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag
     3721 cacgaatctc tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc
     3781 agattttatc ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa
     3841 gtacgattgg agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa
     3901 gaaaagcgcc aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca
     3961 ttatgcggat cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga
     4021 caaacgtttt ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg
     4081 cttcttacgt agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa
     4141 aagtcaatcc aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt
     4201 cacgttcaag tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag
     4261 tagatgatgc aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct
     4321 gtgctggcgt ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg
     4381 tctttagttt ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg
     4441 tagctggagt cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact
     4501 tgccagtagc aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta
     4561 cagcagtaaa cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag
     4621 atttagatcg cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa
     4681 aaagaggttg tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag
     4741 tttctcaaat ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg
     4801 aataagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata
     4861 gttcctcata ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg
     4921 tttaagaaag aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa
     4981 aatagcttct catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca
     5041 ccgtttacca gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata
     5101 taaatacaca ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat
     5161 ttattattaa agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt
     5221 tcccattttc ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc gtttaggagg
     5281 aaacagatgt ttacgaatga aataaaaatc atgctttatg tggacgatgt agaaaaaaat
     5341 gcaacgtttt ggagagcagt tggctttgcg gaaaaagatc gtcaagaaat ggatgggacg
     5401 cttataattg aagtaggagt tgaggacagt caaacctcat ttctgttata tgatcgagca
     5461 tttgttgagt cccattcacc tggcacagct actagtgccc catctttgat gttttacagt
     5521 aaagatatct ttggactata taaaaaaatg cagacgcaat cagtcacatt aggtgatatg
     5581 gtacagcttg gagaagaata tgtctttaac tttgctgatc ccgatggcaa ctactttgcg
     5641 gttacaggta aataatagaa ataaagactt tgtttttctg tgcgaaacag gaaagcgaag
     5701 tctttttaat tcaaataata atattatgta aacaaaaaac tttccatgcg taattataca
     5761 caagcaaata aatcgtcaaa tgcgaacgcc ttctttatac tgtttaggaa cgaaaagaag
     5821 gaaggaagaa atacatggat tatcaagtac tgctttatta caattataca aaaatcgaag
     5881 aaccagagga atttgccaaa aatcatttag ccttttgcaa atctttggat cttaaaggtc
     5941 gtattctagt agctgaagaa ggaataaatg gtactgtgtc tggaaccgtt gaaaacacga
     6001 ctgcctatat gaaagcaatg aaagctgatc ctcgatttgc tgatacgatg ttcaagatcg
     6061 atccaagcga aggacatgcc tttaagaaga tgtttgtaag ataccgtcca gagcttgtaa
     6121 gtttaaagct agaagaggac attgatccaa accaagtgac aggtgcctat ctttcaccgc
     6181 aagaatttaa agaagctatt ttagatgaac agacagtggt gattgatgca cggaatgatt
     6241 atgaatatga cttgggacat tttcgagggg ctgtacgccc agatatacgc agttttcgag
     6301 agcttccaga ctggatccgc gagcacaaag aagaatttat ggaaaaacgg gtagtgacct
     6361 actgtactgg tggcattcgt tgcgagaagt tttctggttg gcttgtccgt gaaggattca
     6421 aagatgtcgg acagctgcat ggcggtattg ctacttatgg taaggatcct gaagtccaag
     6481 gagaattatg ggacggcaag atgtatgtgt ttgatgaacg gatcgctgtc gatatcaacc
     6541 aagtagataa acaaatcgtc ggtcgcgact ggtttgacgg aacaccatgt gaacgatata
     6601 tcaattgtag caatcctgag tgcaatcgtc agattcttgc ttccattgaa aatgaagaaa
     6661 aacatttagg agcatgtagc atagagtgtg cgaaacatcc gcgcaatcgc tatgttcaag
     6721 accatggaat ctctccagaa gaagcagaac tgcggtttac agctttaatt aaatagaaaa
     6781 caatagtttt gattgtcgac catttccaat atgcgctata ctagcgttga ggatggtgat
     6841 ccacatgaaa tcattgattt atgctattgg ggatgtccat ggcaaacatg agctttttga
     6901 aaaacttatt cactattatg atccaaagat ccatcagctt gttttattag gggatctcaa
     6961 tgatcgaggt cctcgtacaa aagaatgttt tttattagga aagcaattag tcgaagaaac
     7021 aggagccatt tacctccggg gaaaccatga agaatatttt ttgcaatttc tgcaatcacc
     7081 ggaggactgg tatgcgcctt atatgagaaa tggtggaaaa gaaacgtttg aaagcctgat
     7141 tcacccaggg gcatcggagg aatactcccc aactgaaatc tcgatgatga tccaatcgag
     7201 atattcagaa ctgattaaat ttttagtcca gcgccccttg tattatgaat gggaaaatta
     7261 tctttttgtc catgccggtg tggatttgac aaagaaagac tggcgtaaga caagtctaag
     7321 agactttatt tggataaggg aaccatttca tgaagggaaa aacaatacag gtaagacaat
     7381 tgtatttgga catacgatta caccaatgct ccatggagat atgcagacaa cagacctctg
     7441 gattcaggat cataaaatcg gaatggacgg tggggctgta ttcggagggt cattgcatgg
     7501 ggttgttttt gacaaggaag gtattcttca agatattgaa tatcagaaca acgaaggacc
     7561 ttggcatgga atctgactaa tggaaagaat gaaggagctg tgactttgtt gcggctcttt
     7621 ttatttttat agagaatatt gtttctttca gtagtttaac ccaggataaa aagtatgata
     7681 gaataaagaa gcaattttta gcagaaaaga ggcattttaa tgacggaaaa tttgaaccag
     7741 acgatcattc agcttgtaca aaaagaactt tcagactatc gtcctaagca gttgactact
     7801 gtacttaatt tgttaaatga aggaaacact gttcctttta tcgcgcgtta ccgaaaagaa
     7861 atgactggaa gtcttgatga agtccagatt cgtgaaatcg aagagcggta tgcctatctt
     7921 gaaaacttag aaaaacgtaa aactgaagtg attcggctaa tcgatgaaca agggaaactg
     7981 actcccgagt tagaagcaga aatcacccaa gccgtgaaaa tgcaacaagt ggaagatttg
     8041 tatcgtccat acaaacaaaa acgacggaca aaagcaacga ttgctaaaga aaaaggactt
     8101 gaaccactcg cgaagtggct gatgcagctt acagatggag aggtgcaatc tgaagcagaa
     8161 aaatatattg acaaagaaaa agaagtctct tctgccgaag aagcgttgca cggtgctcat
     8221 gagattattg ctgaacaagt cagcgataat gcaaaatttc ggacttggat tcggtcttat
     8281 acgtataaca aaggtatgta tgtcagcaat gtcaaggatg agcaagcaga tgaaaaaggc
     8341 gtatatgaaa tgtattatga ctttgcagaa cccgtccata aaatggtttc tcatcgaata
     8401 ttagctacta atcgtggaga aaaagaagat attctgaaag tattctttca agtagatgaa
     8461 gcagcaattt tagcttactt ggatcgccaa atagtcaaaa atcctgcctc tccttcttct
     8521 tcattcgtca gagaagccta tcaggatagt tataaacgct ttattcagcc agcaatcgag
     8581 cgagaactgc gcaatgaatt gactgaaaag gcagatgagc aagcaatcgc tattttcggc
     8641 gaaaacctcc gtaatctttt gttgcaaccg ccactaaaag gtaaagttgt cctgggattt
     8701 gatccggctt atcgtactgg atgtaaacta gcagtcgtag atgcgacagg gaaagtttta
     8761 gcaatcgaag ttatttatcc tcataaacct gcagcgcaag caaaaagaga agctgctgga
     8821 ccagctttta tccaattaat taataaatat caagtagata tggtagctat cggaaatggt
     8881 acagccagtc gggaatctga actttttgtg gctgagcagt tgaaatccgc cgaccataaa
     8941 acctattacg ctatcgtcaa cgaagcaggc gcttctgttt actccgctag cgaaattgca
     9001 cgaaaagaat ttccgcatct acaagttgaa gaaagaagtg ctgtttctat tgcccgtcgc
     9061 ctgcaagatc ctttagctga attagtcaaa atcgatccta aagctgtcgg agtaggccag
     9121 taccaacatg acgtttctca aaaacgtttg gctgaacaat tggattttgt tgtggaaaca
     9181 gcggttaacc aagtaggtgt ggatgtgaat acagcaagcc cgcagctctt gcagcatata
     9241 tcaggattga ataaaacaac tgcccaaaac attgtcattt atcgtgaaga aaacggagaa
     9301 ttcaccgcac gaactcaatt gaaaaaagtg ccacgcttag gaccaaaagc atatgaacaa
     9361 gcaatcggat tcttgcgagt ccctggtggg aaaaacattt tggacaacac aggtatccat
     9421 cccgaaagtt attctattgc aaaagaaata ttaacttcag tccaactttc agaaaaagag
     9481 cttggtacag aagaagcagt agaaaaattg acacgattat ccgtagagaa actggctgaa
     9541 agcctatcag tcggagaaga aacgttagcc gatattttag ctgggctgac acaacctgga
     9601 cgagatatgc gtgatgagat gccagcgccc ttattacgta cagatgtatt gagtatggaa
     9661 gacttgaaac cagggatgga attgactgga acggtccgta atgtcattga cttcggcgca
     9721 tttgttgata tcggtgtgaa gcaagacgga cttgtccata tatcgaaact cagcaaaaaa
     9781 ttcgtcaagc atcctacaga tgtcgtgtca gtaggagata tcgtaacagt ttggatagaa
     9841 caagtcgata cgaaaaaagg cagaatcagt ttgacgatgc tttcccctta tgaagagtaa
     9901 caatgaggag taacaaaaat tgacacaaga agaactgcag cagttagtag aagagatatc
     9961 attaaaaagc ttccagcttc ctttcaagca ccaagcaatg ttcaatccac gcttacgtac
    10021 aactggcggg cgctatcatt taaacgatca tcatttagat tttaatgcga agctatttga
    10081 agagtcagac gaagagacga ttgcaggaat catcaaacat gaactttgcc attatcatct
    10141 tcatctagct ggaagagggt atcagcataa agatatggat ttcaagtatc ttttgaaaaa
    10201 aacaggaggg ttgcgctatg caccagcctt gacgaagaaa cagataaaga tggagtatta
    10261 tcgatgccaa gattgttctg ccacgatctg ccgcaagaga agaatcgatg tacgcaaata
    10321 tcgttgtgga agatgtcatg gagaaatcaa atggattgag acaaaaagag aagactttaa
    10381 tgaagcacaa aaaatcagat gaaaggatag aaggcctttg gaaaaaagtg agcagaggaa
    10441 tctaaaagac tggaatcagc tattaaattt tttagccgca cctgaagagg gaaattttga
    10501 ggaactgacg gcacctacac ttattttagc tggaaactgt cttcctatct tagctgataa
    10561 agcagcacaa atgtatttaa atggccaagt agatcaattg tttttggtcg gaggggtggg
    10621 gcatgcgact agaatccttt atgagaattt tgaaaagcaa ggatttcatt ttgaagatgg
    10681 catgagtgaa agtgaaatct gtcgtcagta tctaaaagaa gtctatgatt tgcccgataa
    10741 ggcttttctg atagaatcga agtctacaaa ttctggagag aacgccactt tttcactgga
    10801 gattcttcat tccttaggag ctgtaccaga aaaaatctta ttgatgaatg atccgacact
    10861 gcaacgcagg actcgagcaa cttttgagaa agtatggcaa aatgaacaaa ctattttcgc
    10921 gaatgctgtg ccatttgtgc cggaaatcct tcacttttct gaagagatca tctttacagc
    10981 aaaagaactg aatcatcaat ggcctaagga atatttccat gcgctagtat taggagaaat
    11041 ggaacgtctc catgacgatg agaacggcta tggtccgaaa ggaaaagatt ttattcctca
    11101 cattgatata tcagaggaag tttggtcttc ttacaccaga attaaagagt caataaaaac
    11161 ggatttttct cgtacttgac cattttagca agcgaatcct cctttccaaa taaatttggt
    11221 tgaaattttc aatcgtttat aagataatga ggagcgtgtt gaatgtaata gctaaaagaa
    11281 tggacgctag ataagagaag aggattcaaa tggaaaatca aaataaattg tatcattttg
    11341 ttggtatcaa aggttcagga atgagctcct tggctcttgt tttacatgaa caaggtctaa
    11401 atgtccaagg atcagacatt gaaaaatatt tctttacaca aagagattta gaaaaagcaa
    11461 atatcacgat tctgccattt aacgcagata atgtaaaacc agggatgacg atcattgcag
    11521 gaaatgcatt tcctgattca catgaagaaa tccagcgagc aaaagaatta ggtttggaag
    11581 tgattcgcta tcacgacttt attggtcatt ttatccaaaa ttatacgagt atcgctgtaa
    11641 caggctctca cggaaaaaca agtacgactg gactgctctc tcatgtattg agtggtgtcc
    11701 gtccaacaag ttatctaatt ggagatggaa caggacatgg tgatccgcaa gcggaattct
    11761 tttcatttga ggcctgtgaa tatcgccgtc atttccttgc ctattcacca gactatgcaa
    11821 tcatgaccaa tatcgatttt gatcatccgg attattatac aagtatcgac gatgtattta
    11881 cagctttcca aacaatggct ggacaagtga aaaaagctat ttttgcgtat ggtgacgatg
    11941 cttatttacg caaattaaaa gcaaatgtgc cgatttatta ttatggtgtc acagaaaacg
    12001 atgatatcca agcacgaaac atcgaacgca caacttctgg ttctgctttt gatgtctatc
    12061 atggggatga atttgttggg cacttcactg taccagcatt cggtaaacac aatatcttaa
    12121 atgctttagg tgttattgca gtagcttatt tcgaaaaact ggatctgaaa gaagttgcag
    12181 aagaaatgct gactttccca ggagtaaaac gtcgattcag tgaaaagatc gtagcagaca
    12241 tgaccgtagt cgatgattat gcgcatcacc ctgcagagat caaagcaacg attgatggcg
    12301 cacgccaaaa atatccagat aaagaaataa ttgcagtttt ccaaccccat acgtttacgc
    12361 gtacgattgc attgatggat gaatttgcag aagcacttga tttagctgat aaagtttact
    12421 tatgcgatat ttttggttcg gctcgggaag aacaaggaaa cgttaagatt gaagaccttg
    12481 gagcgaaaat caaaaaaggt ggagaagtca tcaaagaaaa taatgtatct ccattgttag
    12541 attatcatga tgcagtagta atcttcatgg gtgctggaga tgttcaaaaa tttgaacaag
    12601 cgtatgaaaa acttttaagc agcacgacga aaaatgtact gtaatacaat tactgcagtg
    12661 tccttataaa aatagaaaga gggggagtgg caaaaatctt gccaagcccc cttttttgca
    12721 acttatgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa tcaaatcaaa
    12781 aacatgagaa gctgtttttt cttggctgtt cttattattt gatgcaggac agctttttca
    12841 cgacctctgt aaactatagg aatctgttaa atcaagggag cagaacttgc cttttacgct
    12901 acttttggta caataatgta tgattaaaaa taaactggag ggtttctatc ttgaacatag
    12961 gtaagccgag aaaactagga aaaacaataa atgaaatcga ggaaggcgac tcgttatcct
    13021 taacagaatc tattgaagat aaccagcttt tgctgtattt gggattgacg aatgatgcaa
    13081 atcctttgta tatccaacat gactttgcac aaaaaacaga atatcaacag ccaatcgttc
    13141 catcagtgat gctgatgggg atcatcacaa gtgcaatttc aaagcatctt cctggaccag
    13201 gatcacatgt tgtcaatttt tctgtcaatt ttattgaacc ggttttccat tatgaaacac
    13261 tgacttttca gtttgaggtc ataaaagtag ataagatgaa ggatgtggtg acgatttctg
    13321 tagaagcgga gaatacgaac gaagatcgtg tgttggatgc tgtcgttatg gtccaaccgc
    13381 cacaagttcc tgaattggaa atagcaatcg aaagtgaagg tgaattgaat gaataatcca
    13441 agtacatcaa tgtcaggtat caatcgtttt tatgcaaaag tttatggatt tttaggtatg
    13501 ggacttgccc ttagtgcagt cacttcttac ttggctcttt gggtatttcc tttgcaatta
    13561 gcaactttta tcaataattt tccactaggc tttgtgggtc tgtggatcgt tgaactgatt
    13621 ttagtaatgg ttctaggcgt aaaagcacaa aagaatccga gcttagcaat cggtggattt
    13681 attgcctact ctcttttgaa tggcataaca ttagcggtta ctcttgcata ctacgatatt
    13741 ggaacagtaa cgcaagcttt cttgacagct actttgatgt tcatggggat gtctgttgtc
    13801 ggagtaacaa caaaaagaga tcttagcggg gttggaagag caggacaggc agctttaatc
    13861 ggagtgatca ttgcactagt cttgaacatc ttcattttga gaagtagcgt agttgagctg
    13921 ttcatctccc tactgttagt cgtcatcttt gcagggatta cagcttatga taaccaaagc
    13981 atccgtaata tctatgtcca gacaaacgga acagcacata gtggaattgc tgtattcatg
    14041 gcattgaagc tgtatttgga ctttatcaac ttgttcttgg cgtttctgag aatctttgga
    14101 cggaacaact aaaaaagaaa aaaattcatc agttggggct ataccgaatt atttggtatg
    14161 gtcccgcttt ttcttttcgc attccgcagg attctttgtt agaatgaata agaactttac
    14221 agtaaggagt taagaagatg acaaaaaata aattattact cgttgatggg aacagtgtgg
    14281 cttttcgcgc attctttgcg cttcacaatt ctcttgaacg tttcaaaaat agaaacggtc
    14341 ttcatacaaa tgcaatctat gcattcaata cgatgtttga aaatgttatg caaaaagagc
    14401 aaccgactca tgttctggtt gcgttcgatg caggaaaaac gacttttcgg acagaaatgt
    14461 atgcggagta caaaggtgga agatccaaaa cacctggcga attcaaagaa caaatgccat
    14521 atatacgtga tttgttgaca ggactagggg tacagtatta cgaattgccc aattatgaag
    14581 cagatgatat tatcggtact ttggcagaaa aagtagacaa agatcaattt gatgtcgtcg
    14641 tgctttcagg tgatcgggat ttgactcaat tagctagtaa agaagtaaaa gtggatatca
    14701 ctgtaaaagg tgtcagcgat atcgaaagct atacaccaga acatgtagcg gaaaaatatg
    14761 atgggttgac gccaaaacag attattgaca tgaagggact agccggagat gcctctgaca
    14821 atatccccgg tgtgactaaa atcggtgaaa aaacagctat caagctattg aaacaatatg
    14881 gctcagtcga aggaatttat gaacatatcg atgaaatgaa gcaaagcaaa atgaaagaaa
    14941 atctgataaa tgacaaagag caggctttct tatcgaaaaa attggcgacc atcaatactt
    15001 cagcgcctgt ggatgtctct atcgactcat tgaagtatga agggaaaaat ctcgataaac
    15061 tcgttccgtt ttataaagaa atggatttca atcagttcct ttctaaattg aatatcgtag
    15121 aagaagaagt aaaaatggat gatattttgt ttgaagtcgt tcatgaattc aaagaggaaa
    15181 tgtttactac ggatatggcg ttgtacgtgg aaatgatggg cgacaattat catacagagg
    15241 aaatcgtcgg agtagcgtgg gggacagaaa agaaaattta tgtcacgaac gatctatctg
    15301 tattcgaaaa ccgagcgttc cattcttgga tcactgatcc gactcggctg aaaaaagtat
    15361 atgatgcgaa acgaacttat gttgctttaa accgctatac tggaaaaacg aaaggaatcg
    15421 attttgatgt cttgctaggt gcataccttc tcgatacgaa tgaaaaaagc acagatatcg
    15481 aaggaatagc tgctcattac ggatacaacg atatccaatc agatgaagct gtatacggca
    15541 aaggggcgaa aaaaggttta ccagaagaag aggaactctt ttttgctcat ttagcgcgaa
    15601 aagtagctgc aatcaatgcg ttgacaccaa aattgagcca agaactggca gaaaaaaacc
    15661 aagaagatct tttccggaaa atggaactgc cattgtctat cattttggca gagatggaaa
    15721 taagcgggat caaagtagat gcgactcgct tacaagaaat gaagggcgag ttctctgctc
    15781 gcttaagaga gatcgaacaa aaaatctacg aagaagccgg cgaagaattc aatttgaatt
    15841 caccaaagca attaggagtg atcttatttg aaaaaatggg attacccgtt atcaaaaaaa
    15901 caaaaactgg atattccaca gccgtagatg tactggaaca gcttcgtgaa caagcaccaa
    15961 tcgtagagga tatcctcacc tatcgtcaaa ttgcaaaaat tcaatctacc tatgtcgagg
    16021 gactgctgaa agtgatccaa tcagatggaa aagtgcatac ccgttatgtc cagacattga
    16081 cccaaacagg acgattaagc tctgttgatc ctaatttgca gaacatcccg attcgattag
    16141 atgaagggag aaagatacgt caggcatttg ttcctagaga aaaagactgg ctgatttatt
    16201 cttctgacta ttcacaaatc gagttacgcg tacttgcgca tatctcaaat gatgaacatt
    16261 tgaaagctgc cttcctagaa ggtcaagata tccatgccag cacagcgatg cgtgtatttg
    16321 gaatcgaaaa ggcagaggat gtcacaccaa atatgcgtag gcaggcaaaa gcagtgaatt
    16381 ttggtatcgt gtatggcatc tctgattacg gactttcaca aaatctagga atcagccgaa
    16441 aagcagcaca gcaatacatc gatacgtact ttgaaaaata cccaggtgtc aaagaatata
    16501 tggaaagaat cgtacgcgaa gcaaaagacc aaggctacgt cgaaacgctg tatcaccgcc
    16561 gtcgatatct gccagacatc aattcaagaa attacaatat ccgttctttt gcggaacgaa
    16621 cagctatcaa tacaccaatc caaggaagtg ctgctgatat tttgaaaatc gcgatgatcg
    16681 aattggataa acgattaaaa gaaacaggtc ttcaagctac gatgctgtta caagttcatg
    16741 atgaacttgt cttcgaggta ccggaaaaag aacttgaatc attagataaa ttggtgaagg
    16801 aagttatgga acaagctgtt tctcttcatg taccactgat cactgacagc agttggggaa
    16861 aaacgtggta cgaagcaaaa taaacattgt ttaagacaga gcaatataat gaaggtgaaa
    16921 agaaaaaatg ccagaattac cagaagtaga aaccgttcgt aaaggattgg aacggttagt
    16981 agtaggaaaa acgattcaaa aagttcaggt cttgtggccc aagatcatcg agcaaccaga
    17041 gacacccatc tttgaggctt ctcttgttgg cgaaaccatc caatccattg gacgtagagg
    17101 gaagttttta atttttcatc tcgatcattg tgaactgatc tcacacttgc gaatggaagg
    17161 aaaatatcaa tttacgaaag aaaatacacc aatcgataaa catacgcatg tacttttctt
    17221 ttttgaggat ggaagccaac ttcgctacaa cgatgtacga aaatttggtc gtatgacaat
    17281 cgtcgaaaaa ggagcaagtg caacttaccg aggaatcatg aaattgggac ctgagccttt
    17341 gccagatagc ttcttattag cagatttcgc aaatggactg aaaaagtccc acaaagcaat
    17401 caagccattg ctgctagacc aacgtctagt gactggtcta ggtaatattt atgtagatga
    17461 agcattatgg gaagcgaaga tccatccaga acaaccggcg aacacactgc gccccaaaga
    17521 ggtagaacaa ttacgtctat cgatcattga tgttttggat cgtgcaatag aagccggagg
    17581 taccacgatt cgcagttatt tgaatgcatt aggagaatca ggcggatttc aagtagcact
    17641 gcatgtctat cagcaaactg gcaaaccatg tatacgttgt ggaacgccga tcgtgaaaac
    17701 aaaagtggca caaaggggga cgcactattg tccgaattgt caaaggctaa aaatcgcaaa
    17761 gtaggatttg ttttaggttt gacgggtgga atagctaccg gtaaaagtac ggcagccaaa
    17821 gtctttcagt ctcatggatt tcctttgatc gatggcgatg tgattgccag agaaactgtt
    17881 gagcctggaa cgcttgctct gaggaaaatt gtatcagtgt ttggccaaga ggtattgcag
    17941 cctgatgggc aactagatcg agggaaactt gggatgattg tttttccatc aaaggaaaaa
    18001 cggcaaaaac ttgatcagct attagatccg tttatccgaa aagcgataaa agatcaaatc
    18061 gcctctctaa gttccaatca tcctttagta attgtagata tcccattgct ctacgaaggt
    18121 cattatgacc actatatgga tgcagtagct gtcgtttata ctacgccaga aactcagctt
    18181 cagcgtttga tgaaacgtaa tcaactgacg ctagaacaag cgcagcagag aattagtagt
    18241 caacttccaa tcgaagaaaa aaagcagcgt gcggatattt tgttcgacaa caatggaaca
    18301 aaagaaatac ttgtgggaca aatcgaaaat tggctttttg ctaatcgctt tctttgagta
    18361 tctgtgtcaa ttttgctatc ctgagtatat agaaagtgag ggatagcgat gggatcatta
    18421 aaaaatcaaa aggataaagt cgtaggtaac gcaaaagaaa aaatcggcga gatgctcaat
    18481 aaagaaaatc taatcgatga aggtcgtacg caatcagaaa cagcagaaga aaaagaaaag
    18541 cagtaccaag caagaatcga tgaaatagaa gaccagcagg cagaagaaga agcgaagcgg
    18601 caagatttga ctcaagaacg aaaaaatccg acacctgatc gtagtaaaac accgctccca
    18661 gaaagtgacc acattcccga tgagcaaaaa atcgaggaac agcgaatgaa acactataca
    18721 ggaatcgaag agaagttata aaatagtaaa aaatcgagat gtgacttgaa gcagatgaga
    18781 aggtgttcat ctgcttcaag ttttttgtgc tagatgacta aatcagcttt tgtgtaagtt
    18841 atttctcagt aatcaaggtg atcagtattg gaaaattgaa atttagtaag gacaggggac
    18901 acaattacgc atgaaagtag aaaaaattgt tcttatatcc gtgttttttg ggtaggacag
    18961 tcgcgtttgt gatttgtttc gtgttataat acaactagtt gtaaagaaag acacatcctt
    19021 tatataaaat gcgatgcaag aggtgagaca aaatgagatg tcccagatgt caacataata
    19081 attcacgagt aatcgacagc cgtcaagcag atgatggacg tgcgatccgt cgccgtcgcg
    19141 aatgtgagaa ttgttcgttc cgattcacta catttgaacg aattgaagct gctccgttat
    19201 tagtaatcaa gaaaaacggg gaacgcgaag agttcaatcg cgataaaatt ttaagaggac
    19261 tcatccgttc agcagaaaaa agacctgtag ctatggaaca aatggaacaa atcgtcaacc
    19321 atgtcgaaaa ccgcgtacgc agtctcggag aaaacgaagt atcgacgaat ctaattggtg
    19381 aatatgtgat ggaagattta gttgatttag acgaaatcgc ttatattcgt tttgcaagtg
    19441 tttatcgcca gttcaaagat atgtctgttt tcttaaaaga gttgcaagat atcgtggata
    19501 aagcaaaaga aaataataaa gagaataata aagaaaacaa ttaagaaaca aaaaggaggc
    19561 atttccttgg aaaatgcttg gaaagagctt caaccaaaaa atatttacca aggaagaaaa
    19621 gcggctccgc tgagagaaga cagcaagaac gccttgctgt atctttatca gccactaatc
    19681 ggcggagaag ccttgagtat ctatttcacc ctgttatcag aaatctctca agaatctggc
    19741 aaaggtcctg aagggatgca tgcagatctg cttagtagtc taggctgcgg tatcccgcaa
    19801 ttttatgatg caagaaaaaa gctagaagga atcggcttac tggatgttta ttttaaagaa
    19861 gatagtgatc tgggacctcg ttttatttat gagctgttag aaccactcat tcctaaagca
    19921 tttttcaaag actctgttct ggcttttctt cttctggaaa aagttggtga acgacgattc
    19981 cagaagctta cccaattctt tgaacctaaa aggcatacag cagagggata tcaaaaaatc
    20041 acaaaaaaat ttttagatgt ttatcgcttc aatgaagcag tgtacgctgc taatcaagaa
    20101 aacatcgaat cgctgaataa atcttttgaa gaatctgaat cagtagcatt acttgaagca
    20161 gattcgacat tcgattggca gtttttgaat gattggctgg caaaaaaaca tattgcaagt
    20221 cttgatgatc caatcatcaa acaagtcgac atgtatcatc agctttacgg tttcgatgaa
    20281 ttgacacttg gcgaactgat cagtcaatct tatgacttta gtgaacaaaa agtatctgaa
    20341 aaagaactgc aaaaagccat tttggcaatg cagagacgtc cggtgaagca aacaaaagaa
    20401 acagttgcgg tttctgaaaa cagtaatgaa ccctcgtcaa acgaacattt gacaaacgca
    20461 gcgcttcagc tgataaaaga agcacagagc attccaccga tgaaatatct ggaagcaatc
    20521 aagtcagaaa aaggcggata tgtttctaaa caagaaaact ggttactgca agatcttgtt
    20581 tctcaatcag gtctttccag tagcgtgatc aatgttttgc tgaattatgt actagtgatc
    20641 aagaataatg ctagcttaaa tgccagttat gtaaatacag tggcaaatga atgggcacaa
    20701 aagaaaatcg ccacagcaga agaagcgatc cagcacattc ggcaaataag taatcaagca
    20761 aaacaatcaa aacaaaagaa gcaaacaaat tatcaaactg gcaagcgaaa tgttcgacgg
    20821 gaaaagcttc ctgattgggt caaccagcca aaagatgaaa aacagatcag cagcgagaaa
    20881 aaagcagaga ttgaccgccg ttttaaagaa tacctggcaa aaaaggaggg tgatatctag
    20941 tggaagacgt aggcaaagaa ttgaagagga tcatcagcaa acgcaattat caagaaaagt
    21001 atgctgaaat gatcgctgaa gtcttaaatg atgcggatgt tcaagctttt ctaagcgcac
    21061 accaagaaga attgacacaa gcggatattg aaaaaagtta cgctaaattg tatgaattcg
    21121 tgcaagaaaa gcgtaaatat caaaaaaacg atccaacgat gatcgctccc ggctatgagc
    21181 cgaagctgac gttgaatttt cattatgtgg atgtcactta tgtaccaacg gaagcattgt
    21241 tagcacggca aagacaagaa gaaatcagaa gccgagtaaa agcaatggat atgccaaaag
    21301 atattcaatc ggcttctttt gaaaactttg aacgtacaga tggacgagga tatgcgagct
    21361 tagaagccct agattttgta gagaaatata ctgctgatcc aaaagggtat cataaaggac
    21421 tgtatctagt aggaagtttt ggtattggaa aaacatattt attaggagca acagcgagag
    21481 aattagcgat tgctggattc accacgacgc tagtccattt tccgacattt gccgttgaga
    21541 tgaaacaggc aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac
    21601 ctattttgat gatcgatgac attggtgcag atgcgatgag tagttggata cgagacgaag
    21661 tattcggtgt tatccttcaa tatcggatgc aggaacagtt accaaccttc ttctcatcga
    21721 attttacaat ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc
    21781 cgctaaaagc gaaacggata atggaacgca tacgttattt gacaaaagaa atcgaaatga
    21841 ctggtcgcaa ccgacgcaat ggttgaacag ttgacaagtc caactgacat ttgacctagt
    21901 agtaaaagca ggtcagcgaa tcattatatg gacagaaatg accggagaca atcaccgcaa
    21961 caagtaatgc agaaacttaa acggaggatt ttttatgagg gctatttttt ctagtatatc
    22021 tgtagttggt ctattttttc ttttagcagg ctgcggtagc gcagatagca acacaggttc
    22081 tgttgattcg attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca
    22141 aagcatattt gtcggaacga ttgaaagtat cactcaagta gaagcagaag aagaaacgct
    22201 tcagttgaaa ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg gaacgtcatt
    22261 cagcaatgac ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg acataaatga
    22321 attgaaaatc ggtgacaaag tccaatttac attagttgag atgccgatca tgacgatgtc
    22381 tatcccaccg caagttcctg gagattcgat tattgatgtt tcattgaaca aataaccaat
    22441 aacatttttt caaaaataag aaaatgttgt tttatagaac gaaaaggtat attttttaag
    22501 gagatatggg aattttttta ttcctagtct ccttatttta tttggaaaat gctataatta
    22561 tctagaagga gtgaagctat gaaccattta gatttatctc agcgcttagc cgcagtgggt
    22621 gactttgtcc ctgaatccgc tcgtttagca gacattggat cagatcacgc ttacttaccc
    22681 gttgcattga tattaagagg aaaaatcgaa tatgcagttg ctggtgaagt cgttcaaggt
    22741 ccttatgaat cagcacgtaa gcaagtcaaa aaagatggat tagaagaccg tatcaccgta
    22801 cgccttgcaa atggtctaga tgctatccag ccacaagatg gtattacagc tgtcacgatt
    22861 gcgggtatgg gaggttcact gatccgagat atcctagaag ctggcaagtt gaatcaacgc
    22921 ttaagcggac aagaacgttt gatccttcag ccaaacatcg gagagcgtac attacgatct
    22981 tggctgcaag aaaatcagta taaaatccaa tcagaaaaaa tacttgaaga aaataaaaaa
    23041 atatatgaaa tcattgtagc cgaaaaagaa caaaaaccag ttacttacaa tgaaatggag
    23101 ctgatgttcg ggccatttct gcttgaagag aaaagtgaga tcttcaaatc aaaatggaca
    23161 agagagcttc aacaaagaaa agctatccgt gaacagctca aacaagcagc taatcaagag
    23221 gcacgtcaaa aacaaatcga gcaggaaatc caaatgattg aggaggtttt aaagccatga
    23281 caatcagcgg acgcactttc atccaaaagt ttgaaagcta ttgtcctcag tggctagctg
    23341 aagaaggaga cccagtcgga ctgcatatcg gaacattgga taagccaatc caacgagtaa
    23401 tgatgacatt agacgttcgt ccagaagtgg tcgaagaagc aatcaagaaa aaaatcgatt
    23461 tgctgattgc taaacatcct ccaatctttc gtcctgtaaa acggctggtt actgatcagc
    23521 cacaagaaaa aatgtatgca gatttgttga aacatgatat tgccgtctat gcagcacaca
    23581 cgaacatgga tattatttgg gatggactga atgactggtt ttgtgaactt ctcggtattg
    23641 aagtggagag ttatttagta aagactcatg agattcatta taaaaaactg gccgtttatg
    23701 tacctgtgga tcatgcacag aaaatgcgtg aagtattagc tgcagctggt gctggtacac
    23761 aaggtgatta taccggtaca agttttacct cgattggaca cggtcgattt actccggaag
    23821 ctggtgcaca accagcgatt gggaaagtag ggaagacaga acaagttcaa gaggcaaaag
    23881 ttgaggttat tttgccggaa actatcgaaa aacaagtgat tcaagccatg cggtcagctc
    23941 atccttatga ggaacctgct tatgatcttt tcgctataga tgaaccagtc gaaatgtttg
    24001 ggttaggacg agtaggtgaa ttgccgcaag aaatttctat agaggcattt gtagaacaag
    24061 taaaagaagc gttccaacta gatggactga gaatagtcca gccaaaaaat gcaaaatcct
    24121 ctgtgaagcg cattgcgatt tgcggtggaa gcggtgaaaa attttatccg caagcaatcg
    24181 cccaaagagc agatgtctat atcacgggag atatctatta tcatacggct catgatatgc
    24241 aaagcgctgg gttgattgca attgatcccg gtcattatat tgaatcgtta tgcaagcaac
    24301 gatttatcga gaaatttgaa tcttggaaac aagaagaaaa ttgggatatt aactttttcg
    24361 tttctgaaac aaatacaaac ccatttcaat ttaattaaaa ggagagaata ctatgtacga
    24421 aaatcttttg ccacgttttt tacgatatgt caaaacagaa acacgctccg atgcaacaag
    24481 caaaacgact ccatcgactc agacacaagt tgcttttgcg caagaattga aaaaagaatt
    24541 agaagagtta ggcttagctg atgttttcta taatgaagat aacggattcg ttatgggaac
    24601 attaccaagc aatgttgatc atgaagtgcg ttccattgga tttatttccc acatggatac
    24661 agcagatttt aatgcagtag gagtcaatcc acaaatcatt gaaaactatg atggcgagtc
    24721 tacgatcgta ctagatgcgg aagaacgcta tacattgaat acaaaagatt ttccaaattt
    24781 gaaaaattat gaaggccaca cattgatcac aacagatggc agtacattac tgggagcaga
    24841 tgacaaatca gggattgcgg aaatcatgac tgcaatggag atcttgatca ataatccttc
    24901 tatccctcac ggtaccatca aagtggcttt tggtcctgat gaagaaatcg gtgtcggagc
    24961 ggataaattc gatgttgagc aattcgatgt agattttgct tatacagtcg atggtggtcc
    25021 tttaggtgag ttgcagtatg aaacgtttag tgctgctcaa gcaaatatta cgatccaagg
    25081 gaaaaatgta catccaggga cggcaaaaga tacaatgatc aatgctctac agttagcaat
    25141 cgattttcat aatcagcttc cagccgatga agttccagaa aaaacggaag gatacgaagg
    25201 tttcttccat ttgatggcat tggatgggaa tcctgaagaa gcttctatga gttatatcat
    25261 tcgcgatcat gatcgtgaaa aattcgaagc acgcaaagca caaatcacac atatccaaga
    25321 agagttgaat cagcgatttg atcaagaacg aatcaaagta gatatgtatg atcaatatta
    25381 caacatgaaa gaaatcattg aaaaagatat gagcatcgtg gaattagcga aacaagcaat
    25441 gatcgagctt ggtatctctc cagtgatcga accagttcgt ggaggaacag atggttcaaa
    25501 aatctcttac atgggtattc cgacgccaaa tctatttgcc ggaggagaaa atatgcacgg
    25561 acgattcgaa tttgtttctt tacaagtaat ggaaaaagca acagacgtga ttgtaaaaat
    25621 tgcagaattg aacacaaaga gttaaaatcg aaaaagaaca gcttatgaca aggcgttcgt
    25681 cccaatgatg gaatgtcatc tgtctaaggc taaagggcag gacaatggaa ttggttgtcc
    25741 cgccctcttt tttgaagagt aaataggagg taaagaatgg aaaagctgct agttttttat
    25801 aacaaaagtt cagggaaaga tgagggtgag aaattagcac gctggtttca agattatgta
    25861 aaagaaaagc agccagaatt atatgtagaa ctagctgaaa ctggcccatc gattgatgat
    25921 gcggaattag tcaagaaagc agaagccata gaagctgata ctttagtaat tataggtgga
    25981 gacggcacga ttcatcatat cgtccaagcg ttccaaaaaa cattatctca ataccatgtc
    26041 ggattacttc caggtggaac tgtcaacaac cttgcacgtg ttcttgagat tcccctcaaa
    26101 aaagaagaag ctgctgacgt gatcctggaa ggcaatgtac ggaaaataga ctatggtcaa
    26161 gtcaatgatg aagtcattat ctctacactg acaatcggaa tcttagcaga tacagcagtt
    26221 tggatcagtc aagaagaaaa acagcgttac ggctcgtgga tctttatcaa gaagtttttt
    26281 aagttattgc tgaagaagaa aaaatatcat ttggatttag aaacagacga ggagcattgg
    26341 aaaggaaaat cccaactgtt gacagtcacg atgtctaact ccgtgggcgg ttttacaaat
    26401 tttgacgact cagcgaccct agatgatgga aaatttcatg taacgatcgt accttcctta
    26461 gatattttcc gatttatttt ttatttgcct agaatcatga gaggcaaatt ctatacgatc
    26521 cccggcatta cttatttcac agccaaacgc atgaaaatcc aagcgcagga aaaatctgtg
    26581 ggcactcgaa cggatggaga cacgacagat gatcttccag ttgaattatc tgttgtatca
    26641 aaaggattaa cagtttattc accaaagaaa gaagaaaaag cttagaaata gtagttaaca
    26701 gaaagttgga agaagagtaa tggatgtatt gttacacaat cctttgtggt tttacctttt
    26761 cattgtgaat ttttacttat tttgtctgat ggggtatgat aaacatcagg ctagaactag
    26821 cggctggcgt gttcctgaga ataatttgat atttgtcagc ctgttaggcg gtggtttggg
    26881 aggattgatc tctcgaaaag ttttccatca taaaactcgg aaaaagaagt ttactatagg
    26941 attcgctgca ggtgtacttg ttgatttgat tctcatttat tttttccatt aaggagcagg
    27001 agatgtcgtc taaaggaacg aaaactaaaa tcattctgaa tctgcttttg ctgattgtga
    27061 tttttgccat tatttattat ttaatcaatc aatcatttgc agatatcttt gccgaattgc
    27121 tgtctaccag tgttgttgtt ttggcagcgg ctgttatatt agggacagtt taccaaatcg
    27181 ctgaagggcg ctcaatcaaa gaaattgctg aacctttcgc gccgaatttt acaacgaaag
    27241 atgggttctt gacctctagc tatattgcgt tttaccgaat cgtttcgttt ggtacaggaa
    27301 cgctgatctc ggagatttat ttttataaca aaaaaggaat gaagctatcc cagggagcag
    27361 gggtgacagc actccatatg attatgtaca aactggcagt gatcacttat gcattagctg
    27421 gcttggtcat ccaattctct cttttttatt ctaatggtcc atcaatgatc tggtttatca
    27481 tcgctggtat ccttttgacg atcgtgattg ttgccttttt attggcgatg tccgtcagct
    27541 tgaatgttca aatatttttt gtcagcttat cgaataagtt atttaaaagt gagcgtttgc
    27601 ggaaatgggt ggatacatgc aatcagcaga tctactcttt aagggaaacg gtccaaacca
    27661 ttatccaaga caggactgcg ctattgcgga tttatctatg gaatatgtta aaacttgtat
    27721 tttggtatgt acttccttat ttgattctga ttgaaaacca tcctaaaata gattttctac
    27781 tgaccatttc gtttatcagc tttgcagtag tgctttcagg ggttattcct acgcctgctg
    27841 gtattggttc ctttgaattt gtctatttgt tattattcag accagttgtt ggaacagttg
    27901 acgcagtttc ttcgctttta ctgtatcgtt tcagttcctt tatcttgcca tttctctatg
    27961 gatttgtgta tgtgattgct gaacggcgaa gagtgatcaa agaagaaata atagaagtaa
    28021 aaaaagagaa taaagaaaat caagggtagg tcttttataa gatctaccct ttttatcaaa
    28081 tcaaagaaaa acgttatgga taagataatt ttacgtttgt ttggctcttt ttttatatac
    28141 atacaatcaa ttttgttttt ttaagattta tataagtcga gaaaaaaagt caaaaaacta
    28201 tgtgtatatt ttgaccaaaa ttgaccaaag cgatataata gtttttgtaa agatcaaaga
    28261 aataagtgat cttaccttta attaggaggg tatacctgta tgaatattga gaaaatgact
    28321 acgacgcttc aagaagcgat tgctgaagct caacagattg cggtcactcg aaaacatcaa
    28381 gatatcgaca ttgcccatgt atggaaaatc tttttacaat ctaatcattt tggtcgaaat
    28441 ttctatacgg atgccggatt ggatgtcgaa tcttttgaac atgaaattga tcgtctactg
    28501 gatgagtatc cagttgtttc tggtggaaac gtccaatatg gacaaaacct cagccagaat
    28561 ttctttcgat tattgaatga agcagaccaa atacgtgaaa gcttcggcga cgagtttctt
    28621 tcaacggaag tcgttatttt agcgttgatg aaattaaaaa attatccgtt aactgtttat
    28681 ttgaataaaa acggattatc tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga
    28741 ggagaaagag tgacctcaca aaaccaagaa gaacaataca aagcattaga aaaatacggc
    28801 gttgatttag tacagcaagt caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa
    28861 gaaatccgtg atgtcatccg tattctttca cgaaaaacaa aaaataatcc tgttctgata
    28921 ggagaacccg gtgtagggaa aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc
    28981 aaagatgtgc ctgaaaattt aaaagataaa acgatttttt cattagatat gggtgcattg
    29041 atcgctggag caaaattccg tggtgaattt gaagaaagac taaaagcagt cttgaaagaa
    29101 gtcaaaaaaa gtgatggacg tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg
    29161 gcaggaaaaa cagaaggaag tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc
    29221 ggtgaattac atttgatcgg tgccacaact ttagatgagt accgtcaata tatggaaaaa
    29281 gacaaagcgt tggaacgtcg ctttcaaaag gtattagtca aagaaccaac agtggaagat
    29341 acgatcagta ttctgcgtgg attaaaagaa cgttttgaga tccaccatgg tgttaacatc
    29401 catgacaacg cattagtagc agcagctact ttatctgacc gttatattac tgatcggttc
    29461 ttaccggaca aagcaatcga tttgatcgat gaagccagtg caacgattcg ggtagaaatg
    29521 aactctatgc caacggaatt agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa
    29581 gaagctgcac tgaaaaaaga atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa
    29641 gaagaattag ctgaacttcg tgaagaagca aatgcgatga agatgcagtg ggaaacagaa
    29701 aaagaagaag tcaattctgt ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa
    29761 ttagaagatg cagaaaataa ctatgattta gaacgcgctg ctgtattgcg tcatggtacg
    29821 atacctcaat tagaaaaaga attgaaggaa ttagaagcta aagcaaaaga cagcgagatt
    29881 aagatggttc aagagtccgt taccgaaaat gagattgctc aagtcgttgg cagattaacg
    29941 ggtatccccg taacgaaact tgtagaaggt gaacgagaaa aactaattaa attgaatgaa
    30001 acacttcata aacgagtgat tggtcaagat gaagcagttg atgcggtcag tgacgcggta
    30061 attcgttcac gggctggatt gcaagaccca aatcgtcctc taggttcttt ccttttccta
    30121 ggaccaactg gtgtcggaaa aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac
    30181 tcagaagatc atatggtacg gatcgatatg agtgaatata tggaaaaaca tgctgtgtct
    30241 cgtctagtcg gagctcctcc aggctatgta ggttacgaag aaggtggtca attaacggaa
    30301 gctgtcagac gaaatcctta tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc
    30361 gatgtcttca atatcttgtt acaagtccta gatgacggac gcttgactga ttcaaaaggg
    30421 cgagtagtag atttcaaaaa tacggttctg atcatgacca gcaatatcgg ttcacagtta
    30481 ctgctggaag gcgtgactgc tgatggaacg attccagaag cagtggctga acaagtcaac
    30541 acgctattac gtggaaactt caaaccagaa ttcttgaacc gaatcgacga cacgatttta
    30601 tttacaccat tgagcctaga caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg
    30661 gcacaacgtc tggaacatca agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg
    30721 attgctgaaa atgcttatga accagcatat ggtgcaagac cattgaaacg atttatcact
    30781 aaggaagtag aaacaccact agctaaagaa atcgttgcag gacatgttat gcctaaatca
    30841 aaagtaacga ttactttact tgatggacaa cttcatttca aaacagaaga gctagaagaa
    30901 atcgtctaat cttccattga gactataaaa gaaaaaaggg gtctgacaca actagtgtca
    30961 gacctccttt tttcaaataa acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa
    31021 atttcttgaa gctgcccgct tctgtcacaa cctcttttat taggtatttt tcactttttc
    31081 taatttataa ctttcaaagt aacgctcatc ttttttactt tgagaccatg caaacaatcc
    31141 cattaaggca gcacctatcg tatttgtcag caagtcgccc atcgtatcca tcaatgctgc
    31201 tcgtccgaca aacagtgtgc catcagaagc agcaaaacgt tgaagattca tgcctaagaa
    31261 ctggtcgcat aagaattccc agaactccca aaacacgccg caaagtccag caaacgcaaa
    31321 gccgaataag aggaacaacc aaggactggt ttttgatatc tcagcatctt tcatcaagta
    31381 accaatcaat ccatagccaa gagctgtcag gaccattgga ctcaccgcat gtaagatttt
    31441 gtcccaaaaa gagatgatac tgatcaaatg catccctgtg cctaaaaaga cagagatgaa
    31501 caagaaaaac cagtaaaaca aaacgattgc tgctggtatt ttaagtttga aaactttcaa
    31561 aatgacttgt ggcatgaaaa tcaatgcaat ccctaataaa gattccacga taaatgtgat
    31621 tccttgagaa gtcgaatatt tttgtgtgat aaattcataa aaattatagc agacaacgaa
    31681 tacaccaaaa gcaagtaaag ctatttgcac ctttttgatt tctttaatca ctgaaagtcc
    31741 ctccatctaa aaatcttttt acttctgaaa gcaactcaat ttctgaagaa accacttttc
    31801 cgttcaattt gatcaaacct attgtatata agttcaggta gtgaaattga ttttcagctg
    31861 tttcttgtag tgcagcgatt tttttcgggt gggttccgcc ttgctgtcga gtatcagtat
    31921 ataagccgat cactgggatg cctttcgcat aagccacacc gatttccgat gctaccccgg
    31981 catcgatcgt cagcccatct agtaaagcaa tcattaaatc actagccaac actttttctg
    32041 tgtcagcaag agcaatcatt cgactgtctg cataagcagt tttgtcattg attgcttcat
    32101 tttcttgagg aagatagatc gaaagctcag aagagagctc tctgatttct ttaactaact
    32161 gttggttata taacaaatca cttttagcaa acattggtgc ggcaaaataa attttcatct
    32221 aaacatcatc ctttttatca aaaataatcg ctaaagtaag cttccatgtt tggaatgata
    32281 cgctctagtg acttcagtgt ttcttcatct gtttctaacc gttcgattct tgccaaataa
    32341 gaagcacggc gataattcat catcaagcaa gtcaatgttt ggatatccag tcgaacggat
    32401 tttcctatag gttcatttaa gacacggact tgtccctgtt catcccatgt taaagcgaag
    32461 acaccattat tccaaggagc gacaggatct tcaatgatga aatgaaaagc atcagctgtt
    32521 ccaacaaacg gaaaccgttg aaggaattct tttacatcaa cgatccgagc cataaaatat
    32581 ggttcgatct gctctttgat ctgactatct tcaatcaaaa atgctaaagg ctcattttta
    32641 taaatatctc catgtaccca gtagatcatg gaaaaatgtg cggaaataaa gttccataag
    32701 ccatttcgag cttcttgatc aagatagaac atttctttta cgtgaaatat ttcttcagct
    32761 acccagtaga acagaacacc tcttggttca tggttgctat cgtagtagac agcggctgtg
    32821 cgctcatctt cattttcgaa acgccaatac tcttcccaat gaaaatcact gcggaataaa
    32881 gcaccatggt tttgttgggc aaatttgtca taaacggaaa atacgtctgg atgatcaact
    32941 ggtaatcgct caaccatacc acttactggg atttgtttag gtagctgtgt atctctgatt
    33001 ttaaaggaaa gtttatcaga catgatttcc catcctttac gccgatagta ggggatactg
    33061 taaggataca aataagagat ccattgctta ttcttgcgca tattatccaa tgcaacgata
    33121 attagatctt gcatcaatcc atggttagca tattctggat aagttccaac tccagtcaca
    33181 ccacccattt tataacgtgc accgtgaata ttgacttcac aaggatagat agcgatttga
    33241 gagatcagtt tattttcgtg gaaccatccg aacactttcg atacttccaa aatcggtttt
    33301 ttggaacgga taaaagctcg tttattctca aacccgcttt cctcgatgtc tgcttcagtc
    33361 acttggaaca cataggaaag caattcattg aattgatcga tatgttcttg agtgaccggt
    33421 tttaattcaa gattttctct aaaatctttt tgatccattt tttcatctcc atttattagc
    33481 ttgataacag tatagcaaaa ttctacaaaa aaaacttcta tagctgacta attaaaaact
    33541 ctttaaatta ctgcttttcg ttggtataat ggaaagtgct gattatatga aaagaggaga
    33601 cgctatggat ataaagaaaa tgaaagaacg ccaagagcag attcgtaatt tttcgatcat
    33661 cgctcatatc gaccatggga aatctacatt ggccgatcgt attttagaaa tgacgcacac
    33721 tgtgacgtct cgcgaaatgc aagatcagct gttagactcg atggatttgg aacgtgaacg
    33781 tggaatcacg attaaattaa atgctgtaga actcaattac acagcgaaaa atggagaaac
    33841 atatatcttt cacttgattg acaccccagg gcacgtcgac ttcacctatg aagtctctcg
    33901 aagccttgct gcctgtgagg gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc
    33961 acaaacattg gccaatgttt atttagcgtt agacaatgat ttggagatcc tgccagtcat
    34021 caataaaatc gacctacccg cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt
    34081 aatcggaatc gatgctagtg aagctgtatt agccagtgcc aaatcaggga ttgggattga
    34141 agatattctc gagcagatcg tggaatatgt tcctgcacca agtggagact tagatgcacc
    34201 gctaaaagct ttgatctttg actctattta tgatagttac cgcggtgttg ttctaaatgt
    34261 ccggatcacg gacggtatgg tcaaacccgg agacaaaatc aaactgatga gtaatggcaa
    34321 aacatttgat gttacagagg tcggggtctt ctcgccaaaa gcagttgcta gagacttttt
    34381 gatggtcggt gatgtcggtt atatcacagc aagtatcaaa actgtccaag atacacgagt
    34441 aggggataca gtaacgttag cagacaatcc tgccgctgaa gctttaccag gttaccgaaa
    34501 aatgaatccg atggtttatt gtggtcttta tccaattgat acttctcgtt acaacgattt
    34561 gcgggaagct ttagaaaaac tgcaattgaa tgatgctgct ttacaattcg aaccagaaac
    34621 ctctcaagca ttaggttttg ggttccgctg tggattcctc ggacttcttc atatggatgt
    34681 cgtccaagaa cgcttggaac gggaattcaa tctagaattg atcacgactg caccatccgt
    34741 tatttaccat gttaataaaa cggatggtac gactgctaca gtagataacc cggcagattt
    34801 tcctgaacct gtaacgattc aggatgtaga agaaccattc gtcaaagcac agatcatggt
    34861 cccaaatgat tttgtcggtg cagtaatgga actttctcaa cgaaaacggg gagaattcat
    34921 tacgatggat tatttagatg attaccgagt aaatgtcgtc tataatattc cgctttctga
    34981 aattgtcttt gactttttcg acaaattgaa atcaagcacg aaaggctatg cctcattgga
    35041 ttatgaaatg tctggttatc aaaaaagcaa gttagtgaag atggacatct tgctgaatgg
    35101 agaaaaagtc gatgcattaa gctttatcgt tcaccgagac tttgcctatg aacgtggaaa
    35161 agcaatcgtt gaaaaactga aaaaactaat cccacgccaa caatttgagg tgccaatcca
    35221 agcagctatt ggacaaaaaa tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt
    35281 cttggctaaa tgttatggtg gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa
    35341 agaagggaag aagcggatga aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat
    35401 ggccgttctg aaaatggacg agga
//
LOCUS       DAPBAB010000022        35182 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPBAB010000022 DAPBAB010000000
VERSION     DAPBAB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.022"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..731)
                     /locus_tag="RD444_001340"
     CDS             complement(<1..731)
                     /locus_tag="RD444_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7548723.1"
                     /translation="MRGGVRKRGKRWYYYFEDINDDGSRKKVEKVGGDTRPEAEAALR
                     KVLSDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHIDGYLGK
                     KKLTDLSPALLQNFINDEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVK
                     IPKYEERPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWD
                     NVDFSNGTITVEKQMVKNDGEWVYGT"
     gene            complement(896..1513)
                     /locus_tag="RD444_001341"
     CDS             complement(896..1513)
                     /locus_tag="RD444_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDT7548724.1"
                     /translation="MAKKKVTGEDGKTYVMKEKKPFYKRVWFWILVVIVVFIVGGALG
                     GGDDKKETTSAKTEKVSSNDSAEKDTQSSSDPLKQDFAVGDTVSYEGYEIKVNNVQYS
                     NGSEFDTPDDGKQYVIANITITNNSGKKQSYNPYDFKLNADGNATDMDEITTLDNINQ
                     LNSGDLDDGASVTGDLVGQADTNAKLKLQYATSIWNDETVDISLN"
     gene            complement(1612..2208)
                     /locus_tag="RD444_001342"
     CDS             complement(1612..2208)
                     /locus_tag="RD444_001342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548725.1"
                     /translation="MLIMNLIKKKPKKVGLIITCVSAILFIAGVAVSASTNASTSTAS
                     STSASKTDKSTSEVANSSSTESSDSEDVTFDSEIKEDSTPKEVNPADYNTGITYDTLA
                     RTPDEHIGNKVTLSGEIVQVIEGDDASQYRMAVDQDYDKMVLIEVPTDQLSSRILEND
                     LITIYGVSQGTVDYESTIGGTITVPAITVDKFEVTGQA"
     gene            complement(2336..2764)
                     /locus_tag="RD444_001343"
     CDS             complement(2336..2764)
                     /locus_tag="RD444_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDT7548726.1"
                     /translation="MERDVINLAGKLKQKYNSANPFIICEQMGIQIKYVPFMNNPKGQ
                     FQELLGRSVILLSHELKESEERFYICAHELGHAIFHKGLSSYYVSTRNSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPRKVEELTNLYGLPESVYRFLI"
     gene            complement(2770..3162)
                     /locus_tag="RD444_001344"
     CDS             complement(2770..3162)
                     /locus_tag="RD444_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7548727.1"
                     /translation="MEFGEKLKELRTSKGLGVNQLALKSGVSASQISRFEKGERKDPT
                     LETLKKLSVALGVSISYFEENSPISTSNIPEWADEDDLIELDKILDSNVDMAYGGETL
                     TDEERQRVKDILTGLFWEFRKEDKNKEK"
     gene            3301..3555
                     /locus_tag="RD444_001345"
     CDS             3301..3555
                     /locus_tag="RD444_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7548728.1"
                     /translation="MKTLLKQEKLYSLMQSKGDDPYSLAKRMNVAPSTIYRILNGDRG
                     IGGELIPKLLKAFDLSEKDFDKLFIFSEVLPKNNSKEVSK"
     gene            3736..3888
                     /locus_tag="RD444_001346"
     CDS             3736..3888
                     /locus_tag="RD444_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548729.1"
                     /translation="MTRQEKISIVLDARPRLVHIIKCANDDQLDRLVEEVNKDLQREL
                     DEAAFV"
     gene            3952..4497
                     /locus_tag="RD444_001347"
     CDS             3952..4497
                     /locus_tag="RD444_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548730.1"
                     /translation="MFKLLKKSSVIRESLVEVINKSGETKKAIARQINVSQQSLSDWT
                     TLLNTKPVTLENAQALTDHFRDSDFTLQVIHEFFGLFKSIDGDVYRRDPSSLDKLQMI
                     ESDERKQKKQEVEKILLKQVNYLTVDDRQQIIAYAYEFLDEIMVEVTLISALCEILGI
                     DIRKLSEERLSYWVVQGYMKG"
     gene            4494..4781
                     /locus_tag="RD444_001348"
     CDS             4494..4781
                     /locus_tag="RD444_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDT7548731.1"
                     /translation="MMEMETLENIFPKKVVLKRNSKRNVEKLTYSVTEAALAITTNPQ
                     NVKALIDMGYLGCLKIGEIRIPKAEVNRFLNNYMNRDLASEIAKYREERKK"
     gene            4778..4963
                     /locus_tag="RD444_001349"
     CDS             4778..4963
                     /locus_tag="RD444_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548732.1"
                     /translation="MKTVFKMTVKSALLMSLVAIVLASINPAYALIYWGTLVAVTAVR
                     ESFKMPTQKRPTSDGNR"
     gene            5100..6041
                     /locus_tag="RD444_001350"
     CDS             5100..6041
                     /locus_tag="RD444_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDT7548733.1"
                     /translation="MSKSTLEMSRQEWLEDRQKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEETASEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     DVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSEATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKRKASIGITQKNIISWKLVS
                     TKRVNSKKLAEKYPDIANDEEIYNVTESRRLTEKEIK"
     gene            6043..6933
                     /locus_tag="RD444_001351"
     CDS             6043..6933
                     /locus_tag="RD444_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDT7548734.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6982..7875
                     /locus_tag="RD444_001352"
     CDS             6982..7875
                     /locus_tag="RD444_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDT7548735.1"
                     /translation="MDYIGQLNAFDNWLEYNELGAGPQLLWYKLMAIANKSGWQSELS
                     IANTRLQAMTKTSEKTLINNRNQLIQNGLLQYKKRGRTKAGVYILSDLTGNFTVKTTV
                     DNTVENSATGNIPVDSKVNPKVNREVNPSVDSTVNPSAYINNTKQNKTNKEDDDIGVY
                     EFIQKNWGKAPTGLLQGALGPMIKTWGADMILFAFKLAFENNVEMTGLKKYVEAILNS
                     WSNQGIKTMESAEKAQEAFKNKKKQNYLPKRQNNVRREKLPDWVNKPQEEKTLDPDKK
                     AELEARFAAYQAKKEALLENE"
     gene            7862..8227
                     /locus_tag="RD444_001353"
     CDS             7862..8227
                     /locus_tag="RD444_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDT7548736.1"
                     /translation="MRTNKYRNRKTIHRGIKFDSKAEAEYYDLALWQAQANGWKVKLQ
                     ERFELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQY
                     RVPLILAKKYRNTFKEERF"
     gene            8242..8403
                     /locus_tag="RD444_001354"
     CDS             8242..8403
                     /locus_tag="RD444_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7548737.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            8400..8705
                     /locus_tag="RD444_001355"
     CDS             8400..8705
                     /locus_tag="RD444_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548738.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKPMWAEKTK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFKYVEEK"
     gene            8705..9022
                     /locus_tag="RD444_001356"
     CDS             8705..9022
                     /locus_tag="RD444_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDT7548739.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDRKSKERAELVKLEMAKTGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILDEYME
                     DSK"
     gene            9019..9237
                     /locus_tag="RD444_001357"
     CDS             9019..9237
                     /locus_tag="RD444_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HDT7548740.1"
                     /translation="MITLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            9250..9531
                     /locus_tag="RD444_001358"
     CDS             9250..9531
                     /locus_tag="RD444_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548741.1"
                     /translation="MGDMSEYWKDIKPHLKERRKQHVRKMGNSATKNIEALGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            9528..9854
                     /locus_tag="RD444_001359"
     CDS             9528..9854
                     /locus_tag="RD444_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548742.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENITDFMIN
                     DAIDRLRFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEAK"
     gene            9855..10076
                     /locus_tag="RD444_001360"
     CDS             9855..10076
                     /locus_tag="RD444_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548743.1"
                     /translation="MFKIAFYLFDYTDDSFKKVYFHHWNDSKPVFTKNKRRAQEYFDE
                     RSANKDIVQLKKAESPSAKTLSIRLEEEE"
     gene            10073..10369
                     /locus_tag="RD444_001361"
     CDS             10073..10369
                     /locus_tag="RD444_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548744.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMELINDK"
     gene            10435..10614
                     /locus_tag="RD444_001362"
     CDS             10435..10614
                     /locus_tag="RD444_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548745.1"
                     /translation="MELKGIELSDIEKMQGDHCAIIISNGQMKSVKLPPFGTIVIESH
                     CNKVKQVKEEVKQLF"
     gene            10718..11305
                     /locus_tag="RD444_001363"
     CDS             10718..11305
                     /locus_tag="RD444_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="positive control factor"
                     /protein_id="HDT7548746.1"
                     /translation="MNDLIQEYKNDLKMLKKQYQKILNKRYRIPVKEHGRVIHKLIDD
                     RSPQDIADQKVIAEAISTTEYALFWLETGREKPFDDEQAKKIPKHRRAVKLADIDVMS
                     YQVYLQEVEKPAEETISPKKKEMLLQVTEIESLLSNKELTLFHLINKDLCTYGEAAEQ
                     MNLAVGTVKSMSQRIKNKIDNYFEYGHQINLFEIC"
     gene            11568..11981
                     /locus_tag="RD444_001364"
     CDS             11568..11981
                     /locus_tag="RD444_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548747.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDHRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(11974..12165)
                     /locus_tag="RD444_001365"
     CDS             complement(11974..12165)
                     /locus_tag="RD444_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDT7548748.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQN
                     INSIKNGITKDSQVIDVTK"
     gene            12716..12979
                     /locus_tag="RD444_001366"
     CDS             12716..12979
                     /locus_tag="RD444_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548749.1"
                     /translation="MDNEKFITKCKQLVADYANENFDKTDQTQITMDDVFVVWSCKTL
                     QNNKALLSTTVSDGMYYELTYNGDKSEIYFDAYKKWENKAIKV"
     gene            13020..13244
                     /locus_tag="RD444_001367"
     CDS             13020..13244
                     /locus_tag="RD444_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548750.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLLYCGHGFYSDKHIQENLSKYV"
     gene            13287..13997
                     /locus_tag="RD444_001368"
     CDS             13287..13997
                     /locus_tag="RD444_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548751.1"
                     /translation="MKKYELAKDDYEKGLKYREIAEKYGVSISTVKSWKSRYWSREKV
                     ATKNATIPNNKGAPEDNKNAVTHGLFANWLPSETLEIMNKVATSKPEDILWNNIMIQY
                     TAIIRAQKIMYVDYEGSLSKEVSKWSSSDSGSSEEYAIQYAWDKQANFMNAQSRAMST
                     LSSLIRQFVSIADEHDERRKKLEYMDVQVNLAKAQLKQLDDGYDSSEEQTVIIDDIPL
                     VESEAGSNGINSQETNSN"
     gene            13963..15288
                     /locus_tag="RD444_001369"
     CDS             13963..15288
                     /locus_tag="RD444_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDT7548752.1"
                     /translation="MALIAKKQTQIKTTDLINPHFYKVWHAKCPYILMKGGRGSFKSS
                     VISLKLATEMKKHTQAKHKVNVVCMMSQHKYLRDAVYEQIKWALSMLGISKEYKFRTS
                     PLRIIHKRTGSKFYFYGVDDPLKLKSNAIGDVIALWYEEAANFESEEVFDQTNATFIR
                     QRSPWVDQVQVYYSWNPPKNPYDWVNEWVEKCMRLNDHLVDHSTYKDDELGFTDPQQL
                     KLIETYRENDEDYYRWLYLGEVIGLGTLIYNMDHFHPLDELPDDDYIVQICFSIDSGH
                     QISATTCGCYAITKKKNVILLDTYYYSPEGKVNKKAPDELSKDLHDFIERCQTKYNKY
                     AYKITIDSAEGALKNQYYKDYNERFHAVAKAKKVDMIDYVQNLLAQGRFFYLDTEANR
                     IFIKEHRDYRWDEDTLQSDDPKVIKVGDHTCDQFQYFVKDNLSDLGLKW"
     gene            15282..16841
                     /locus_tag="RD444_001370"
     CDS             15282..16841
                     /locus_tag="RD444_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7548753.1"
                     /translation="MVGEKMSGIQRIKNFFKKGVKRIDMNLNGIELGKITDHPKIGID
                     SLEYARIAENFKYYANLFPDVNYKSSFGDNKKREFKSLNVTKTAARRLASIIFNEKCK
                     VTLNDPTDKKDVSKEIKEASEFLEQTLYDNNFYNLFELNLEKGIAAGGFAMRPYIDGD
                     KIKISWIRADQFYPLRSNTNEVSECAIATKSIQTEGDTNYYYTLLEFHEWQDEKYVIS
                     NELYKSDNSNVVGKQVPLSILYPDLAETVTLEGLKRPLFAYFRTPGANNKSLESPLGV
                     GIVDNAKEILDTINNTHDQFAWEIQMGQRRVVVPAEFLKTDEAHPPMFDSDQNVFVGM
                     YGAENAGIKDITTAIRTVQYKDAISHLIKEFEVQVGLSVGSMNYADDGIKTATEIVSN
                     NSMTYQTRSSYLTMVEKVINELIHSIFELAGYGEMFESKKPLFSIEYDSYLVTVSFED
                     GLFVDRNKQLENDLKAVTAGVMPKKQFLIRNYNLNEDELEDWLAALKDEMPEAGSTER
                     RSQDALFDLGD"
     gene            16844..17980
                     /locus_tag="RD444_001371"
     CDS             16844..17980
                     /locus_tag="RD444_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDT7548754.1"
                     /translation="MITPEKMQKAANSIINIYSELEDRIFNIIIKALKQSRFQDVAKE
                     DVLLWQVNQLSKMGTLNDKVIDLLASYTGETQEAIEQLIKGNGVKIVDEIDRELERMV
                     HKSVPVSDDVNKILDSLVRQTFQDLNNNVNQTLITTNFNENAVMRAYQAILKQSTIES
                     MTGLKTHEKAVKDNVYKMVDMGIKSGFVDKAGREWSMEAYSRTVIQSTSHRTFNDLRL
                     KRMEDFDCVTALMSSHPVAREACAPIQGGWVLTVPKNEAPEEFKHLPSIYDHGYGEPG
                     GTQGINCTHILYPGRPDVNTNNQPRYDPEEAQRNAEIQQKQRRLERDIRYQKKRMSAA
                     VELEDPETVQMCKQVIANKQKQLRELINEHEFLVRDYSREQVQS"
     gene            18091..18666
                     /locus_tag="RD444_001372"
     CDS             18091..18666
                     /locus_tag="RD444_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDT7548755.1"
                     /translation="MKREELKELGLTDEQIGSIMALHGVTVNELNSRVSTAEQQATQY
                     QEQLEKNQNELNDFKANAKGNEDLTKQLEDLQSRFDEIKTSSEQQIADLKKSSAIDLA
                     LTQAGAKNIKAAKALLDSESLELTDEGLKGLDDQLAALKESDGYLFGQSEQVPPNPDG
                     KKATFSGNASSAQNNEEDAFAKALGIMPNKN"
     gene            18684..19556
                     /locus_tag="RD444_001373"
     CDS             18684..19556
                     /locus_tag="RD444_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548756.1"
                     /translation="MAINYITKDNGIFDQKITQGLLTTILGIPQVEFVNGGKSFTLTT
                     ISTSGLKYHTRNKGFNSGTYGNDKKVYTMGQDRDVEFYIDKQDVDETNQDLAVANISN
                     VFITEHVQPEIDAYRFSTLALGAGNTKEETITEKNAYTAIKAAILPARKFGPQNLVAF
                     VSTTVMDALERSSEFTRNITNQNVGQTALESRVTSLDGVLLVEVWDDTRFKTKFDFTD
                     GYAAAADAQDINILVVAKQAVIPVVKENTVFLFAPGEHSQGDGYLYQNRLYHDCFIKE
                     QQKEGVSVSLAPKA"
     gene            19574..19819
                     /locus_tag="RD444_001374"
     CDS             19574..19819
                     /locus_tag="RD444_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDT7548757.1"
                     /translation="MNKTTATLTVGATETLSATVSPETATDKSVKFTSSDETIATVTP
                     VQGKVTAIKVGATTVTATTVNGKTATCEVTVTAASEG"
     gene            19857..20252
                     /locus_tag="RD444_001375"
     CDS             19857..20252
                     /locus_tag="RD444_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548758.1"
                     /translation="MSYLTHEEYLKSGFNKVSVSEFDDLEKCAERQLNRVTGDFYMRH
                     SLADDTFKYRVDKFKIAMAVQIEYLKSVGVTSLSDLLNASPSSVSVGRMRIESGSTNA
                     ATVGRTMVATEAYNELIYTGLLYKGVDYR"
     gene            20249..20596
                     /locus_tag="RD444_001376"
     CDS             20249..20596
                     /locus_tag="RD444_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDT7548759.1"
                     /translation="MIPLMPKELCNQSITLRLLDGHDKWQKPIFSDPIMINHMIFQPQ
                     TVYSGSNNNRQVVANAIAFLFAGVSDPMPVINKNHVGSKIDFEGETYTITTIVDNRNP
                     YSNEVYSYELEVL"
     gene            20596..20931
                     /locus_tag="RD444_001377"
     CDS             20596..20931
                     /locus_tag="RD444_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDT7548760.1"
                     /translation="MLHVKVEKNGVDRKLSVMNINSALYYMTSQMHMDMNLYVPKRQG
                     NLRDKSFVNKNRITYTVPYAKPQFRGIVNGSRVKNYTTPGTSRRWDLRAKANHMDDWR
                     RAFIKGGNL"
     gene            20931..21323
                     /locus_tag="RD444_001378"
     CDS             20931..21323
                     /locus_tag="RD444_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDT7548761.1"
                     /translation="MDLWERLSDSIDSIQGLPMPCSMGFLNGEDTLCVYSMPGSRTVE
                     EYFDGTKEREMLYEVGFNTKDQEKANQTLWLISNHLDELSTLKSEDESFVFLGIEISE
                     TPFVSEQDTQGNSTYLLGIKITIHQFKN"
     gene            21336..21803
                     /locus_tag="RD444_001379"
     CDS             21336..21803
                     /locus_tag="RD444_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDT7548762.1"
                     /translation="MAENSKEFLLNFKNKLEIDTSGSTDLDQIASAKFAPLAAGITTI
                     TPAAADTTDASPYYDGEGFTESTVTGKNITFQVAGHRVFGDPAQDYVASKFLSIGDEL
                     RTLAQWTDAKGNKVQAVVTLTSIVPFGGAANAKQTFSFTMAFNGKPKTVAAGE"
     gene            21803..22060
                     /locus_tag="RD444_001380"
     CDS             21803..22060
                     /locus_tag="RD444_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDT7548763.1"
                     /translation="MSPTSVKLNKTTLSLVVGATETLTATVLPANATNKNVTWSAVDS
                     TIATVDAKGKVVAVKAGTTEITVNTVDGNKSAECALTVTAL"
     gene            22120..22554
                     /locus_tag="RD444_001381"
     CDS             22120..22554
                     /locus_tag="RD444_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548764.1"
                     /translation="MAINNIIDLDAKLSLTKSMKIAGKVYEVQISDEIDKTLTDLTTI
                     DIPDQLKNMTSKLEKMDEDDNGAKEFKEFTQSEMDELKDKAVTTLDVILGSGEGNRVY
                     NFYNKSTKALFTIIGLLEKELGEVVSERSKTAKKHYKNNRKK"
     gene            22559..23179
                     /locus_tag="RD444_001382"
     CDS             22559..23179
                     /locus_tag="RD444_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548765.1"
                     /translation="MFDLTRKPETTVIISGNEYQIDLSFDTVIRFYELIDDKNLESIE
                     KIILGFKLFYIDSKKAEDTFTFEEMQQAINDIVDYIQSNPYGSIGSEGEPTGQDSNMN
                     YSYSQDAGAIYSSFMADYKIDLLNEQGSMHYLTFKALMSGLSEDTQFQRILAIRSRSI
                     AGLEGEELNSLLELKNYYALDSEKTVNSLDDQLGDMFSMLAAQAKS"
     gene            23192..27682
                     /locus_tag="RD444_001383"
     CDS             23192..27682
                     /locus_tag="RD444_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDT7548766.1"
                     /translation="MSADATINIDVMLSNLPKFKTDVSFVDDVLTKLGMNTGSKIDDS
                     FKAETAKVTTIAKSTKKDVDQTFDKPVKFTIKADNSDAEKDVKETKAFLKGIPKSKIT
                     ELKADNDGASLKIKATKEGISKIPNRKETILNADASQAKTETKDLGDTAEKTKSKFIS
                     LKDKLSIGAIAGASSQALQILTGSFSDLIGETAQSSDAMDKFKSTMQFAGFTEKETKE
                     AAKFVKKYADDTVYELSDISNTTAQLAANGIGNYQELTEAAGNLNAVAGGNSETFKSV
                     AMMLTQTAGAGKLTTENWNQLADAIPGASGKMQEAMKNNGAFTGNFRDAMEEGQISAE
                     EFSKAIVDLGMTDVAEEAAKSTKTFEGAMGNLQANIVTKMNEIVDGIGKDKLTDIISF
                     ISDNITKLFDSILKGIDYLNDNQDKLSSIFDNLKKISKIFFGAAWDAVLDIIKKLTGN
                     FSLLSKETGKTKDPLQRFDDVLSALSKHEKGIALLGKALATLFIAKKIVGVVSAFGKL
                     LDMFGGKALLSSPIFLIPAAIAGIGFAFYKAYKTSKPFREFIDGIVDAVKNFVEVSLK
                     KIKSFFKNVTEGFVEFKDSVTDKVTSIKKSIKKVFNSVIDFFKEDWKEVLTFILNPIA
                     GVVALLYKHNKKFKKWVDDLLDTIKDGLKAFKKKVLDPPYDAITDLIDKIAKTFSKWM
                     GKIEKALEKAGKKLGKATEVIFWLIYGPIRLLGRKIKKGFDEVAEWIEDKLEKAGKAV
                     GKVVDKISSTIKKIFNSLSKSVKKSMELFTEYVIEPVDSVRKKVVKTISNLVDKVVDF
                     FVDLVNITKHKWREIKSSMEKPVDDAKENVSKTISKLKEKIAEIFDRIKSTTKSAWNT
                     IKRYTVEPIEDAYKKVVEKVGEIYSGVTKYWNDLKDKTKEKFDEIVDYVKKVPGRIGD
                     AFKDGKEAIGDGIKAAAQYMVDILGTGVNGVIKGINWVLEKVNAPDSVRVDKWDVPKF
                     ATGGIHQGGLMLVNDGEGEELVRHPDGRMEIPKGKNVLMHAEAGTQVLNHSQTKSFAE
                     AFGIPMYAKGNVSNLGDFFKSAWNGIKDIGSDILDAVQHPVEFVKKAISEHVNFDATH
                     PVFDIATGGVKKVTNGVMDWIKDKIASFGSIGGSFDGAMADNVYKYLVDIANQTVSKF
                     GMSGITSGYRPGDPYYHGKHQAIDIAYPAGMNGSSKYFDPANWVFEHFADKVGYVITQ
                     GKVRDRTGQSGQPATGNWETWPDNDHYDHLHITGKLGSGDIYKAGEGGGKGSPTGSGV
                     ARWTSQLKEALRMNGLPTTASYVNAWLRQIETESGGNERAVQPGIDPDGDGSGPAMGL
                     IQAKKGTFLANAFPGHGNIFNGFDSMLAGIRYALKRYGPDMLAVIGHGHGYSNGGEVF
                     GPELAMFGEDPAYPYEININPAKPSADMLIQKAIVARDRYKPAVQTNQAGYSNQHPSG
                     GNEHKTMSKKDIETIVQALNERPVRVESILDGKKVSKSVDEYTGSSLARKLYTRGKNF
                     NG"
     gene            27675..28502
                     /locus_tag="RD444_001384"
     CDS             27675..28502
                     /locus_tag="RD444_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDT7548767.1"
                     /translation="MDDKTSVFLQFSTGKFDLLANYRIKIIDIKIGMPVPKNEFSSYA
                     GSVGKKLLTHSFDSFPITFEFDYFADNLNDLILTETELRELFNKEAEYYFIYTKEPGK
                     RYPVIVESMTVTKKAYFKGNCVVSFSAYKGYSESMATTLSDFSLDEDWQFSQGLVSED
                     FSYTHNTSFFKIFNAGSFEIDPRESDLRITLEGESEGNVTIFNKTTGDRFIYYPSLST
                     NLGQTLVLDGVYPKLNGVSCGIDTNHGLITLAEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            28511..29554
                     /locus_tag="RD444_001385"
     CDS             28511..29554
                     /locus_tag="RD444_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7548768.1"
                     /translation="MKNILIRNYEETKEEILINYDKDSFSVSWQQNETWELSVTVPET
                     KRNQITFDLIDYENYVVFDGQQYSIKQMRPYASGSQIYKDVVATHVYYTIQDGWQYDT
                     ISGTKSINDLLTHIFKAGNRGFSWEVVDPNNVFLKKEQENFGNDNYLNLINEILEDYG
                     AVVIPNNKHLVFYPISEYGNITEQQIRYKYNTDEVSFDIDTYALKTQIKGYGKLKENA
                     NTDNLKDSDYVFPPVTYTSAESEKWGIRIQDPVEDERYTIQNNMLVYLKQQLHDYPDI
                     SGSVTLKWAVSLNKGDKVLFVYEPLNISTYIQVVGITDYPAIPNKAPEIVLSNTKKTI
                     TSILANLAKKGLM"
     gene            29555..30946
                     /locus_tag="RD444_001386"
     CDS             29555..30946
                     /locus_tag="RD444_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548769.1"
                     /translation="MGLIKLLSNNIALKWKETFNQNVDYLNNLEKKLSDQDKSTNSRI
                     DNLVLHSGGDSPNEVVDARINAEGTIYPTLYSRLLALDNLFNLNYTELKTRQDNQQGQ
                     LNQLNVSVGTLMGAYGETLDLYVAKTGSDQSGDGTEKNPFLTIQAAVNQIPLLTSSRV
                     TIWIGDGVYLEDVVIRNLKAVSITLRSRQSVTDATSDLSVKVRSISFISSLGYQQVNG
                     IEFVDQANISGAAYIDGDIKCAIYSEQSSYLGVWNCRFAENTFGKGNRCLFAIGASKI
                     GTSNNFYQNQNCIAEARNLADINIDSNDRGSGNDYGVIADNGTARVKVAGSKVKANRI
                     AEVRNQGNVVTGKIIRQITNDDISDRDNITNVNGTIKREGDTVTIAIKYECNNYPSDA
                     SNTRNVILVPAGFQRDQSYPAYHPLALYRNETQPAGARAGLTQASRVVAYSGNGSSYI
                     SGTWVTNDPIPII"
     gene            30962..33061
                     /locus_tag="RD444_001387"
     CDS             30962..33061
                     /locus_tag="RD444_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDT7548770.1"
                     /translation="MFKINESIIVIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKNG
                     IPQSLPEGTTVPIRLMFKSATAEGGYGKHDYLATVEDPVNGIVSIVLEDNILGYVGTV
                     EGSVYIDFPNDRSLDTAGRFTFYIKRSPIDDSTPELENYYFNGFSQTIDKIEKILADG
                     KQEIDQKITESETQIDAKLKDTSNKITKANQDVATINTNIDKANDRIDQTNQQISDLG
                     KLKKMYSNSIDFGNYDYSGNPNLMANINADSFSQGSGALSVVDDGDEVVITLDPNHKL
                     EIFKAKSQPALVVGKTYTMSVEIMLEDDFTGDPSNISLRYIKMPSWLTVLYTPYTLTN
                     TKGVWQKLTVTAKMTTAIDNAESWFIMLQNKDANNSLSGKLRLRHAKLEEGSTATPFQ
                     PNLLAEPYNMCREYPNENIADPKVKFPIESGDHQIYQGYTEEELMIGQTYTITLKGTK
                     PASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQYPRSTVGAC
                     QIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNNAVNVYDP
                     QRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVVVNGT
                     VKFKKAYAFGVPLDDEVPDEFIARIVHGMLTGQSGTNSVSKAMYVQKDLGKIMTNSEF
                     AANEWFTFHGNYWVGVK"
     gene            33062..33508
                     /locus_tag="RD444_001388"
     CDS             33062..33508
                     /locus_tag="RD444_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548771.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            33510..33647
                     /locus_tag="RD444_001389"
     CDS             33510..33647
                     /locus_tag="RD444_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDT7548772.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            33682..33924
                     /locus_tag="RD444_001390"
     CDS             33682..33924
                     /locus_tag="RD444_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDT7548773.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            33940..34137
                     /locus_tag="RD444_001391"
     CDS             33940..34137
                     /locus_tag="RD444_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDT7548774.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            34148..35167
                     /locus_tag="RD444_001392"
     CDS             34148..35167
                     /locus_tag="RD444_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDT7548775.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT        12396 a         5791 c         7169 g         9826 t
ORIGIN      
        1 gtaccatata cccattcgcc atcattcttt accatttgtt tctctacagt aattgttcca
       61 tttgaaaaat cgacattatc ccacgtcaga ccacaaactt cgccaacgcg cattcccgta
      121 taaaatccaa tattcaaagg aatatagaaa ggatggcctt caggagtgat ttctagcata
      181 tgatcaaagt cctcaagaga aatgattttt agatcttttt tagtcgttgg tcgttcttcg
      241 tattttggta tctttacata cagcatagga ttttgcttaa ttaaccccca aggataaacc
      301 gccatattca gcgcattctt aaggacagag tgagtaatag tcattgtttt cttcgagtaa
      361 ccctttttaa attcatcatt gatgaaattt tgtaaaagag caggggagag atccgtaagt
      421 tttttctttc ctaaataacc atctatatga tttttgatgg taaatcggta gttttcatag
      481 gtattgtatt ttagatttag tttaacgtat tcctccatcc aaaaatcaag gtattgtttt
      541 actcgagtat ccgtacctaa aaagtattgt cctgtttcgt caatatctga taaaactttt
      601 cgtaaagcag cttcggcctc tggtcgggtg tctccgccaa ctttctccac tttttttctt
      661 gagccatcat cattgatgtc ttcaaaataa taataccaac gttttccacg ttttctcaca
      721 ccgccacgca tgttatcaat cctttcgtag atgatttgta atagtaagaa attttataat
      781 atttattttt aatctgatag gggatagagc aaaaatcgta ttcataaata aaacatacga
      841 acttatgttc ttttgtgctt aaaaagaaaa gcccgaaggc tgttcttttg atctattagt
      901 ttaaagaaat atcaactgtt tcatcattcc aaatagatgt agcatattga agctttaact
      961 tggcatttgt atcagcttgg ccaaccaaat caccagtaac tgaagcacca tcatccaaat
     1021 cgccactatt taattggtta atattatcta atgtagtgat ttcatccata tcagtggcat
     1081 taccatctgc atttagcttg aagtcataag ggttatatga ctgcttttta ccagaattat
     1141 tagtaattgt aatgtttgcg ataacgtatt gttttccatc atcaggggta tcgaactcac
     1201 taccattact gtattgcaca ttgttaactt taatttcata gccctcatag ctcacagtat
     1261 ctccaacagc aaagtcctgt ttcaaaggat cactagaact ctgtgtatct ttctcagcag
     1321 aatcgttgct acttactttt tctgttttag cagaagtagt ttctttttta tcatcgccac
     1381 cgcctagcgc accaccaacg atgaacacaa cgattaccac tagaatccaa aaccaaactc
     1441 tcttatagaa tggttttttc tctttcataa catatgtttt accatcttct cctgtaacct
     1501 ttttctttgc cattatttct cctccaaaaa aatattatta ctcccactta atggcaggta
     1561 gtgatagtcg ccattaataa aaagaaaagc ccgaaggctg atctttaagt tctaagcttg
     1621 tcctgttact tcgaatttat ctacggtaat agcaggaaca gtaattgttc ccccaatagt
     1681 agattcataa tcaacagttc cttgagatac tccgtaaata gtgattaagt cattttctaa
     1741 tattctacta gaaagttgat cagtagggac ttcaattaga accattttat catagtcttg
     1801 atctaccgcc atccgatatt gagaggcatc atctccttca ataacttgaa caatttctcc
     1861 agatagtgtc actttatttc caatatgttc gtctggtgtt cgagctaatg tatcataagt
     1921 aattccggta ttataatctg ctggatttac ttcttttggt gtagaatctt ctttgatttc
     1981 actatcaaaa gtgacgtctt cgctatctga actttccgtc gaacttgaat ttgctacttc
     2041 tgaagtagac ttatcagttt tagaagctga tgtagaagaa gcagtactag tagaagcatt
     2101 agtagaagct gagacagcaa ccccagcaat gaataaaatt gctgatacac aagtgatgat
     2161 taaaccaact tttttaggtt ttttcttaat taaattcata attaacataa ttaaaccaac
     2221 tataaaacct aataaaccaa ctattgataa aaatattgct aaacccataa taaataaaca
     2281 cctaacctat tctatttttt tactcccact tagggcagga agtagtagcc gccaattaaa
     2341 ttaaaaatct atacacactt tcaggaagcc cgtacaaatt tgttaattcc tcaactttcc
     2401 tagggtattg atcgttgtct tctttataaa gagaaacaat gagattagca gcaaagcaat
     2461 tcgcttcgct ttctgatttg cttctagaat ttcttgtaga gacgtaataa cttgataatc
     2521 ctttgtggaa aatggcatga ccaagctcat gggcgcaaat ataaaaacgc tcttctgatt
     2581 ctttcaattc gtggcttaag agaatgacgg atctccctaa taactcttga aattgtcctt
     2641 tcgggttatt cataaatggc acgtacttaa tttgaatgcc catttgttca caaatgataa
     2701 aaggattagc agaattatat ttctgcttta atttaccagc tagatttatt acgtctctct
     2761 ccataaaaat cacttctctt tgtttttatc ttcttttcta aattcccaga acaaaccagt
     2821 taaaatatct ttaacacgct gtctctcttc atctgttaaa gtttcaccgc cataagccat
     2881 atctacgttt gaatcaagta ttttatcgag ttcaatcaaa tcatcttcat cagcccattc
     2941 cggaatatta ctggtactaa taggtgaatt ttcttcaaaa taggaaatag aaacgcccaa
     3001 agctactgat agttttttta aagtttccaa ggttggatct ttccgttcac ctttttcaaa
     3061 tctagatatt tgagaagcac ttactccaga ttttaacgct aactgattaa ctcctaatcc
     3121 tttagaggtt ctcaattctt ttagtttttc tccaaattcc atgataaaac ccctttctaa
     3181 ataaatgata tagccttgtg gcaataaaag caaaaaaaaa tgcaatttgg caacaaaaag
     3241 tgttgccaag tgacaaaaaa agtattatag tattgtcata aggcaatgga ggtgtattag
     3301 atgaaaactt tgcttaagca agaaaagctc tactccttga tgcaatcaaa aggagatgat
     3361 ccatattcac ttgctaaaag aatgaatgtt gctccgtcaa caatttatag aattttaaac
     3421 ggggatcgcg gaatcggtgg agaactcatt ccgaaattgc ttaaagcttt tgatttatct
     3481 gaaaaagatt tcgataagct ttttattttt agtgaagtat tgccaaaaaa caacagcaag
     3541 gaggtttcta aatgaaattt ttaataatag caatatggat atttggaata ctttgtattc
     3601 agtcaatgcg taaattagaa cctagcaata gttggtaccc tttctacgca ttgctagttt
     3661 tattaataat tactagttat ctattatccg tctaatatcg tacaaaagtg agttaaaaac
     3721 aagggggact tccaaatgac acgacaagaa aaaataagca tcgttttaga tgcaagacca
     3781 agactggttc atatcatcaa atgtgcgaat gacgatcaac ttgatcgttt agttgaagaa
     3841 gtcaacaaag accttcaacg tgaactagac gaagcagctt tcgtttgatt ctttaaatta
     3901 atagtataaa aaaattgctc gtattgatat acgggcgaat aagaatatga ggtgtttaaa
     3961 ctgttaaaaa aatcaagtgt tattcgagaa tcgttagtcg aagtaattaa taagagcggt
     4021 gagaccaaaa aggcaatagc aagacaaatc aacgtctctc aacagtcatt aagcgattgg
     4081 acaacattgc ttaatacgaa gcccgtgacg ttggaaaatg ctcaggcgtt aacggatcat
     4141 tttagagatt cagatttcac tcttcaagtg attcatgagt tctttggttt atttaaatca
     4201 atagatggtg atgtttatag aagagatcca tcttcattag acaagttgca aatgattgaa
     4261 tcagatgagc ggaaacagaa gaagcaagaa gtagaaaaaa ttcttcttaa acaagtaaat
     4321 tacttaactg ttgatgatcg tcaacaaatc attgcatatg cttatgaatt tttagacgag
     4381 atcatggttg aagtcacact aataagtgca ttatgcgaaa tacttggaat cgatattcgc
     4441 aagcttagtg aggaacggct gtcgtactgg gtagtacaag gatatatgaa aggatgatgg
     4501 aaatggaaac attggaaaat atttttccaa aaaaagttgt cttgaagcgc aacagtaaga
     4561 gaaacgtcga aaaactaact tactcagtta ctgaagcagc attagctata acaacaaatc
     4621 ctcaaaacgt aaaagctttg atcgatatgg ggtacttggg ttgcttaaag attggagaga
     4681 tacggatacc caaagctgaa gttaaccgtt ttttaaacaa ttatatgaac cgagatttgg
     4741 ctagtgaaat tgctaaatat agagaggaga gaaagaaatg aaaactgtat ttaaaatgac
     4801 tgtcaagagc gctttgctta tgagtctagt agcaatcgta ctggcaagta ttaatccagc
     4861 atatgcactt atttattggg gaaccttagt agcggttact gctgtaagag aaagtttcaa
     4921 aatgccaaca caaaaaagac cgaccagcga cggcaatcgc taatcggcaa cataacaaaa
     4981 tatcttatct gtattttagc atgaaaggaa ggctaaaaca atgaacgatt ttggacaagc
     5041 attagatcag tatttaacta ctccagaatg gggcacacca cacgaagagg aggaagacga
     5101 tgagtaaatc taccttagaa atgagccgtc aagaatggct tgaggatcgt caaaaaggta
     5161 ttggtggttc tgatgttgga acgattttag gattgaacaa atggaaatca ccttatcaat
     5221 tatggcttga aaaaacagga caagtcgtac ttgaagaaac agcaagcgag ccagcttatt
     5281 ggggtaatgt tttagaagaa gtggttgcta aagaatttca agaacggaca ggtaaaaagg
     5341 ttcgcagaag aaatcaagtc tttgaacatc cactgcatcc gtttctaaga gcgaacatcg
     5401 atcgtgatgt tgttggagaa aacgccattc tggaatgcaa aacagccaat caatttctcg
     5461 gtaaagaatg ggaaggcgaa gaggtaccgc ttagttatct ctgccaagtt cagcattaca
     5521 tgaacgttct aaacaaagac tattgttaca tcgctgtctt gatcggtggc caaaaattca
     5581 tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag
     5641 agttttggga aacgaacgtt cttggaggta tcgagcctgt aattgacgga agtgaagcga
     5701 ctgctgactt cttaaaagaa aaatatgcag atgtagaaga aaatcaaaca gctctaccat
     5761 cacgttttga tgaacttatc gagcaaaaaa gagaactcaa gcggacgaaa aaagaaattg
     5821 agtcagctat ccgtcaagta gataacgaga ttatcagcga gctaggaaaa cgtaaggcaa
     5881 gtatcgggat cactcaaaaa aacatcatca gttggaaact tgttagtact aaacgcgtga
     5941 actcgaagaa actagcagag aaatatccag atatcgcaaa tgatgaagag atttataacg
     6001 ttactgaatc aagaaggcta accgaaaagg agatcaaata atatggcaac aaatgaatcg
     6061 ttaaaaaacc aattggcaga aaagccacag aaacaagttg caccaggaca gttagggctt
     6121 aaagctctaa tgaatacacc aacaatgaga aagaaatttg aagaagtact tcatgacaat
     6181 gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat
     6241 agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta
     6301 gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga
     6361 aaatgggaaa agaaagctca atttatgctt ggctataaag gatatatcca attagcccaa
     6421 cgatcaggta aatacaaagc attaaatgtg attgaagttt acgaaggaga actaaaaagc
     6481 tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacatc tgatgaagtc
     6541 attggatatg ttggctattt tgaattactg aatggattca agaaaactgt ctattggacc
     6601 aaacaagaaa ttgaagctca tcgaattgct aacaataaag atcgcgacaa aacaaagtta
     6661 agtggtgtgt gggcgtctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt
     6721 ctttctaaat gggggatctt gtccatcgaa atgcaagaag ctaccacatc ggatgagaga
     6781 gtccaaagag ttcaagaaga tggcagcatt attgctgaaa cagaagttga ggaagatatt
     6841 cctgaaagaa aagaagcaga ggttatttct gaagaaaacg aagatgtaca aactggatta
     6901 tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gttttctccc tcaaattact
     6961 agaacgaaag gaggaacaca attggattac atcggacagc ttaatgcttt tgacaattgg
     7021 cttgaatata acgagcttgg cgctggtccc caactgcttt ggtataagct aatggctata
     7081 gcaaacaaaa gtggatggca gagcgaatta tcgattgcca atacaaggct acaagcaatg
     7141 actaaaacgt ctgaaaaaac attgattaac aatcgtaatc aattgatcca aaacggactc
     7201 cttcaatata aaaagagagg tcgtacaaaa gctggagttt atattctttc tgatctaact
     7261 ggaaatttta cagtaaaaac tacagtagat aatacggtag aaaactccgc tactggaaat
     7321 attccagtag atagtaaagt aaatccgaaa gtaaataggg aagtaaatcc ttcagtagat
     7381 tctacagtaa atccttcagc ttatataaac aatacaaaac aaaacaaaac aaataaagaa
     7441 gatgatgata taggcgtgta tgagttcatc caaaaaaact gggggaaagc acctactgga
     7501 cttttgcaag gagcattagg accgatgatt aaaacttggg gagcagatat gattctcttt
     7561 gcttttaaat tagctttcga aaacaacgtt gagatgacag gattgaaaaa atacgttgaa
     7621 gcgatattaa attcatggag taatcaagga attaagacaa tggaatcagc ggaaaaagcc
     7681 caagaagctt ttaagaacaa gaaaaaacaa aactatcttc ctaaacgtca aaacaatgta
     7741 cggcgtgaaa agttacctga ttgggtcaac aaacctcaag aagaaaagac gctagatcct
     7801 gataaaaaag cagaattaga agcccgcttt gctgcttatc aggctaagaa ggaggcgctt
     7861 cttgagaacg aataaatatc gtaatcgaaa aactatccat cgaggtatca agttcgattc
     7921 aaaagcagaa gcagagtact acgatttggc actgtggcaa gcacaggcga atggttggaa
     7981 agtgaagctc caggaaaggt ttgagctgat gccgaaattt gaactagacg gaaagaagta
     8041 tcgcaagatc gagtatattc ccgacttcac attttataaa aacggcaaac ttgtcaaagt
     8101 cgtagatgtt aaagggatgc agacaaaaga ctttaagatc aaggcgaaac tcttctgcag
     8161 tcaatatcga gtaccactga tcttagcgaa gaaatatcgg aatacgttca aggaagaacg
     8221 gttttaacga ggtgatccat catgacaaca gaagaagtga ttcaaatgcg aattcgaagc
     8281 attcaacgtg aaattgacga tctggagcgg acaaaggcag tgatggtcaa tgaaacggct
     8341 agaaaggcaa tcgatttgca catagagaac ttaagaaggg aaattcgtag attggaggaa
     8401 tgagcgtgga taagaaagca gcaatgaaac gaattgctga attaaccaag tcagaatctt
     8461 ggcaagaaga caaagaaata gttgcagaag tccaaaagct tggcaaacca atgtgggctg
     8521 aaaaaaccaa acggaaaacg ccgagaaaaa ttgcaatctg gcatggtgat cgaattctag
     8581 taacaggtac tgctgaacag ttatctgaaa ttactggtct gagcaaaaac atcatctggg
     8641 atagagcgaa gaatatggat attgattcta aaggacgtca atttaagtat gtggaggaga
     8701 aataatggat ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaaaat
     8761 tcagaaagat agaaaatcaa aagaacgagc ggaattagtt aagttggaaa tggctaaaac
     8821 aggagcagga gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta
     8881 caacgaaatt caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag
     8941 caagaaactt catcaagatc gtttcaaatt tgtagaaaag tatagagaga tattagacga
     9001 atacatggag gacagcaaat gatcacccta acaatatttt ggtgtgtaat tggcggctta
     9061 ctagcgatgt taggagcata cctcatttac gagagtgcaa aaggcaaaaa atatacagca
     9121 gtagggatta ttttgtttgt tgtcggaatt ttagttgcga ttggtgcaga aataaatctt
     9181 tatttaaacg gtacgacaga agagttgatg tggtttttat tgtggacaag gagataggag
     9241 gacagcgaaa tgggtgatat gagtgaatac tggaaagata ttaaaccgca tctaaaagaa
     9301 cgtagaaaac aacatgtcag aaagatgggc aattcagcaa ctaaaaatat tgaagccttg
     9361 ggatttgaat ttactcatta tccaaacaat catcaatttg caattaatac ccataatgga
     9421 attattgact actggggaac tacaggaacg tggattgagc gcaaaacaaa aaaacgtggt
     9481 aaagggatac gtagtttgag gaaatatatt aatcaggagg acagcaaatg aactatacac
     9541 aacaagaact gactgattta tgtccaaaac atgtggctga gttcatcaat aatgaagtcc
     9601 tgcctaaata tgcagatggt ttaaatacag cagaaaacat tacagatttt atgattaatg
     9661 atgctattga tcgtttgaga tttttagaaa tagattgtat tgcttattat cgtctacacg
     9721 cagaagttgc tttgattgat ccgtatattg ctttaagcca aaatcgaaaa atactcgtag
     9781 cctatattga taccaaacag attttagaag gcagagatgt cgagatagag atcgatgagg
     9841 aggaagcgaa atgaatgttc aaaatagcat tttatctgtt cgattacaca gatgattcgt
     9901 ttaagaaagt ttatttccat cactggaatg atagcaagcc agtttttaca aaaaacaaga
     9961 ggagagctca ggagtatttt gatgaaagat cagcaaataa agatatagtg cagttaaaaa
    10021 aagcagaatc accatctgca aaaacattgt cgattcgatt ggaggaagaa gaatgaaact
    10081 aaaagacgga ttttacgcta gcagtcacgg catcggcggt ttaatgctag atatgccgac
    10141 aaagaaccct aaaacacgta agaaaccaaa agtcaaagtc ggtgacatgg ttcgctgcga
    10201 agcagaagga ttcatctatc catttcgtgg atatgtagag catctctata atcactcagc
    10261 aatcattcgt attgaaaaca cgatggaatg cgataagtgg ttagcgaaaa gcaaagagaa
    10321 tttagcagtg gctcgattgg tggatatgga actaatcaat gacaaataaa aaagccggat
    10381 cgctccgact gatgtaataa atccgacaag tttattatat cacaggaggc agctatggag
    10441 ttaaaaggaa ttgagttaag tgacatcgaa aaaatgcaag gagatcattg tgcaatcatc
    10501 atttcaaacg ggcaaatgaa aagtgtaaag cttcctccgt ttggaacaat agtcatagaa
    10561 tcccattgca ataaagtcaa gcaagttaaa gaagaagtga aacaattatt ttaaatatcg
    10621 tcctaccaga aaactggcgg acacaagttg acaagaactt tcttgttgat ttgtgtccgc
    10681 tttttttgtt tgcgaaatta ttgaggaggc tatctatatg aatgatttaa ttcaggaata
    10741 caagaatgac ttgaaaatgt tgaaaaagca atatcaaaaa atcttaaata aacgatatcg
    10801 catacctgtc aaagaacatg ggagagtgat acataaatta attgatgatc gttctcctca
    10861 agatatagca gatcaaaaag taatagcaga agctatctct actacagagt acgctttatt
    10921 ctggttagaa acaggcagag agaagccttt cgatgatgag caagccaaga agataccaaa
    10981 gcatagaaga gccgttaaac tggcagatat agacgttatg agctatcaag tttatttaca
    11041 ggaagtagaa aagccagcag aagagacgat ttctccgaag aaaaaagaga tgttgctcca
    11101 agtgacggag atagaatctt tgctttctaa taaagaactg acattatttc atttaattaa
    11161 taaagattta tgtacttatg gagaagcagc agaacaaatg aatctagctg tgggtactgt
    11221 taaatctatg tcacagcgaa ttaaaaataa gatcgacaat tattttgaat atggtcatca
    11281 aatcaatcta tttgaaattt gctaaaactt gtaaaccatt cccacctata agtgaagaca
    11341 attaatagat tagacactca caagtttatt cattctttta ttctgaataa ttgttcttca
    11401 aaaataaaac gcaagggagg gaatctccct catcgtttta aattaagctt cgatagacag
    11461 cagcggaaat attaagaata aggatgtgaa tttcaactcc ttctaaatta ttcttattat
    11521 ctatcatccg ttgctgtctg ttaatttatg tattggaggg aaaacgaatg gataaagaaa
    11581 tcaaagcaac tgtcaaatta gatttgactg aactaaaaga actgctcaac aaggctagtg
    11641 accaagtcga acaaataaag tatcaaggta tggatcgaaa acggttgatt gatattattg
    11701 agcaacaacg tttagaattg atccaaaaag aagctgtatg taaagagtac aaaaaacatt
    11761 tagaacaagt aattgagtat cattcagtag aaaagtacag atcagtggtg caaaaaaata
    11821 gagcaacgag tcctgatcat cgtcaagatg tatcgattag tatcaagacg ccagtattta
    11881 attctgttaa atcagaaaag ctagaaagta tctcaactgc tttggtagaa aaagcagctg
    11941 aaatgaactc cctagcaaat gaagttagtc gtcttattta gtcacatcga taacttgaga
    12001 gtctttggtg attccgtttt ttatagaatt aatgttttgt tcagcggaat atttagtcaa
    12061 gtatgtttcg ctagttgcta ctacttcatt gttattggac ttaataacga agtaatattg
    12121 gccattagtt gcttctctta taacaaagta caaatttttc accacctttt aatttatttc
    12181 agcggaccac tcgctgataa ctaaaattat acgcttagta tttattttca caatattaat
    12241 ttgtcactgt ggcggaaagg gtagacgcta agcatgcgtg ctaggtcaat acttcggcaa
    12301 ctatgcaagg ttcgattcct tgccagtgac ttaaggaacc tacggaaaca attcatctta
    12361 tcggatgccg atgaattggc tgactagtcg ggatgctact agcagttaga aggcataaaa
    12421 tactagcgca gacgtgcgcc actctcaggt gtaggttagg agagaaacat tagttggggt
    12481 tattaggaat acgataacct gcttgcgaca aagctttgta ctgtcgcgtt ggtcatgaac
    12541 agagacggta ttctgtttca gtattcgtta gcaaccgagg gttggaaatg ggcgctcaaa
    12601 gtacacgagc aaggcgaggt cgatagtaat cgatggaatc ggtgtaggtt gctattacat
    12661 attagatcac tctttgagtg gtctttttat tttagaaaag agaggatttt gaaaaatgga
    12721 taatgaaaaa tttattacta aatgcaaaca attggtagca gactatgcaa atgaaaactt
    12781 tgataaaaca gatcaaacgc agattacaat ggacgatgta tttgttgttt ggtcctgtaa
    12841 gacgctacaa aataataaag cgttgctgag tactacggta tcggacggaa tgtattatga
    12901 gctaacgtat aacggcgata aatcagaaat ctattttgat gcctacaaaa aatgggaaaa
    12961 caaagcgatc aaagtctagc acatgctagg ctctttcttt acataaggga ggctgcataa
    13021 tgagaaacta ctggtatgta tcactaacta atgaatatcc tcgaaccatt gatgattgtt
    13081 cagtgcgtgt tgtgcgttct gtacaaatca aagggaagta ctctattgtc gaaatgctaa
    13141 gagaagctac accaaacgaa gtggataaat gcaaacttct ttattgcggt catggtttct
    13201 attcagataa acacatacag gaaaatcttt caaagtatgt gtagattaca aaacaaatgt
    13261 tgcaaacaag cgaggtggtg tcacatgtga agaaatacga actagctaaa gatgattatg
    13321 aaaaagggtt gaagtataga gaaatagcgg aaaagtatgg tgtatccatc agcacagtaa
    13381 agtcatggaa atcccgttac tggtctcgag aaaaggttgc aaccaagaac gcaactattc
    13441 cgaacaacaa aggagcgcca gaggacaaca aaaacgctgt tacccatggc ctttttgcca
    13501 attggttacc ttctgaaaca ttagaaatta tgaataaggt cgcaacctct aaacctgagg
    13561 atatcttatg gaataatatc atgatccagt acacggctat tatccgagca cagaaaatca
    13621 tgtatgttga ttatgagggt agtttgtcca aagaagtttc taaatggtcc tcgagtgatt
    13681 ctggaagttc agaagaatat gctattcaat atgcttggga caagcaagct aatttcatga
    13741 atgcacaatc aagggctatg agcacgctat ctagcttaat tagacaattt gtctctatag
    13801 ctgatgaaca tgatgaacgt agaaagaaac tagaatacat ggatgtacaa gtcaatttag
    13861 caaaagcaca attgaagcag ttagatgatg gttatgattc gtcagaagaa caaactgtaa
    13921 ttattgatga cattccgtta gttgaaagcg aggctggttc aaatggcatt aatagccaag
    13981 aaacaaactc aaattaagac tacggacttg attaacccgc atttttataa agtttggcac
    14041 gcaaagtgtc catatatttt aatgaaaggc ggacgtggct cttttaaatc atctgttatc
    14101 agtctgaaat tagcaacaga aatgaagaag cacacacaag ctaagcacaa agtaaatgtt
    14161 gtctgcatga tgagtcagca caagtattta agagatgctg tttatgaaca aatcaagtgg
    14221 gcactttcaa tgcttggcat ttcaaaagaa tataaatttc gtacttctcc tttgagaatc
    14281 attcataaac ggactggatc taagttctac ttttacggtg tggatgatcc cttaaaacta
    14341 aaatcaaatg caattggcga tgtcattgct ttgtggtatg aagaagccgc aaactttgaa
    14401 agcgaagaag tatttgacca gacgaatgca acctttatcc gtcaacggtc gccatgggta
    14461 gatcaagtac aagtttacta ttcatggaat ccacccaaga atccatatga ctgggttaat
    14521 gaatgggtag aaaaatgcat gcgcctaaat gatcatttag tggaccactc aacttataaa
    14581 gatgatgagt taggttttac ggatccgcaa caattaaagc tgatagaaac gtatagagaa
    14641 aatgatgaag attactatag atggctttac ttaggcgaag tgattggtct gggtacgctc
    14701 atctataaca tggatcattt ccatccgctt gatgagctac cagatgatga ttacatcgtt
    14761 cagatttgct tttcaatcga tagtggacac caaatatcag cgactacctg tggttgctac
    14821 gctattacca aaaagaaaaa tgtcattcta ttggatactt attattattc tcctgaagga
    14881 aaagtaaata agaaggcacc tgatgagctt tctaaagatt tgcatgattt tattgagcga
    14941 tgtcaaacaa aatacaataa gtatgcctac aaaatcacta ttgattcggc ggaaggcgct
    15001 ctaaaaaatc aatactataa agattacaat gagaggtttc atgcagtggc caaagctaaa
    15061 aaagtggata tgatcgatta tgtacagaac ctccttgcac aaggcaggtt tttttattta
    15121 gatacggaag caaatcgaat atttattaaa gagcatcgtg attatcgatg ggatgaagat
    15181 actttacaat ctgatgatcc aaaagttatc aaagttggtg accatacatg tgaccagttc
    15241 caatattttg taaaagataa tctaagtgat ttaggactga aatggtaggt gagaaaatga
    15301 gtgggattca gcgcatcaaa aactttttta agaaaggagt gaaacgaata gatatgaatt
    15361 tgaatggcat agaacttggt aaaatcacag atcatcccaa aattgggatt gattcgctag
    15421 agtacgctcg aatagcggag aacttcaaat attatgctaa tttatttccg gatgtcaact
    15481 ataagagttc atttggcgac aataaaaaac gagaatttaa atcattgaac gtgactaaga
    15541 ctgctgcgag acgattggct agtatcattt tcaatgaaaa atgtaaagtt acgctgaacg
    15601 atcccactga taaaaaggat gtttctaaag agattaaaga agcatctgaa tttttggaac
    15661 aaacattata cgacaacaat ttctataatt tgttcgaatt gaatcttgag aaaggtatcg
    15721 ctgcaggagg atttgctatg cggccttaca ttgatggaga taagattaaa atctcgtgga
    15781 ttcgtgcaga tcagttttac ccgttacgtt caaatacaaa cgaagttagt gagtgtgcta
    15841 ttgctactaa atcaattcaa actgaagggg acacgaatta ctactacacg ctcctcgaat
    15901 ttcatgagtg gcaagacgaa aagtatgtta tcagtaatga gctttacaaa tctgacaaca
    15961 gtaacgttgt tggaaagcaa gttccactgt cgattctata tcctgactta gctgaaacag
    16021 ttacactaga aggcttgaag agaccgcttt ttgcttactt cagaacgcct ggagctaaca
    16081 ataaatcgtt agagagccca ttaggcgttg gaatcgttga taatgcaaaa gaaatcttag
    16141 ataccatcaa taatactcat gaccaatttg cttgggaaat acaaatggga caaagacgtg
    16201 ttgtagttcc tgcagagttt cttaaaacag atgaagccca tccgccaatg tttgatagtg
    16261 atcaaaatgt atttgtaggg atgtatgggg cagaaaatgc tggaataaaa gatattacta
    16321 cggcaatacg aacagttcag tataaggacg ctatcagtca tctaattaaa gagttcgagg
    16381 tgcaggttgg tttgtcagtg ggttcgatga actatgcgga tgacggcatt aaaacggcca
    16441 ctgagattgt ttctaacaat tccatgactt atcagacacg ttcaagctat ttgactatgg
    16501 ttgaaaaagt tatcaatgag cttattcatt ctatttttga acttgcagga tacggagaaa
    16561 tgtttgaaag caagaaaccg ctattctcta ttgaatatga tagttattta gtaacagtta
    16621 gttttgagga tggtctattt gtcgatcgta acaaacaatt agaaaatgat ctaaaggctg
    16681 taactgctgg cgtaatgcct aagaaacagt ttcttatacg taattacaat ctaaacgaag
    16741 atgaattaga agattggtta gctgcattaa aagatgaaat gccagaagcg ggatcaaccg
    16801 aacggcgtag tcaagatgcc ttattcgatt taggtgatta attatgatta caccagaaaa
    16861 aatgcaaaag gccgcaaact caattatcaa tatctattca gaactggaag accgaatctt
    16921 taacatcata atcaaagcgt taaaacaatc tcgttttcaa gatgtagcta aagaagacgt
    16981 gcttttgtgg caagtaaatc aactttctaa aatgggtaca ctgaacgata aagttattga
    17041 tttgttagca agttatactg gagaaactca agaagcgatt gaacaattga ttaaaggaaa
    17101 cggcgtgaag atcgttgatg aaattgatcg agagttagaa cgaatggtcc ataaaagtgt
    17161 tcctgtatct gacgacgtaa acaaaattct agactctttg gttcgtcaaa ctttccaaga
    17221 cttaaataat aatgtcaatc aaacgctgat cactactaat ttcaacgaga atgcagtcat
    17281 gagagcttat caagcaattc tcaaacaatc aaccatcgaa tctatgacag gtcttaaaac
    17341 gcatgagaaa gccgtaaaag ataacgtcta caaaatggtt gatatgggaa ttaaatcggg
    17401 ttttgttgat aaagctggtc gagagtggtc gatggaagct tactcgagaa cagtgattca
    17461 atccacctca cacagaacgt ttaatgatct tcgtttgaaa cgaatggagg acttcgactg
    17521 tgtcactgca ttaatgagta gtcatccagt agctcgcgaa gcctgtgcgc caattcaagg
    17581 cggatgggta ttaactgtgc cgaaaaatga agcgccagaa gaattcaagc atttaccatc
    17641 tatttacgat cacggatatg gcgagcccgg tggaacgcaa ggaattaact gtacacatat
    17701 tctttatcct ggtaggcctg atgtgaatac aaacaatcag ccgcgatatg atccagaaga
    17761 agcacaaaga aatgctgaga tccagcagaa gcaaagaaga ttagagcgtg atattcgtta
    17821 ccagaagaag cgaatgagtg cggcggtaga attggaagat cccgaaactg tccagatgtg
    17881 caaacaagtg attgctaaca aacagaaaca gttaagagag cttatcaacg aacatgaatt
    17941 cttagttcgt gattacagta gagaacaagt acaaagttaa taattttagg cttagcaatc
    18001 gctagtcttt ttattttgcc ccgaatacgg cgttaaactg ttcaatccat cgagggcgta
    18061 gcctcgttaa acaacgaaag gatgaatgaa atgaaacgtg aagaactgaa agaacttggc
    18121 ttaactgatg aacagattgg atcaattatg gctttgcatg gtgtgactgt gaacgagttg
    18181 aatagtcggg tgtctaccgc ggaacaacag gccactcaat atcaagaaca gttagagaaa
    18241 aatcaaaatg agctgaatga tttcaaagca aatgctaaag gaaatgaaga tcttactaag
    18301 cagttagagg atttacaatc taggtttgat gaaatcaaga cgagttctga acaacaaatt
    18361 gctgatctta aaaaatcatc agcgattgac ttagctctaa cacaagccgg agctaaaaac
    18421 attaaggctg ctaaagcctt gctagacagc gaatcattgg aactgacaga cgagggatta
    18481 aaaggattag atgaccaact ggccgcgctt aaagaaagcg acggttattt atttggccaa
    18541 tctgaacagg ttccacctaa tcccgatggt aagaaggcta ccttttctgg gaatgctagt
    18601 tctgcgcaaa acaatgaaga agatgctttt gctaaagcat taggtattat gccaaacaaa
    18661 aattaaattg gagggaataa aatatggcaa ttaattacat tacaaaagac aatgggattt
    18721 tcgatcagaa gatcactcaa ggattgttaa cgacgatctt aggtattcca caagttgaat
    18781 ttgtgaacgg tggtaaatca tttacattaa cgactatttc aacttctggt ttgaaatacc
    18841 atacacgtaa caagggattc aacagcggta cctatggaaa cgacaaaaaa gtgtacacaa
    18901 tgggtcaaga ccgtgacgtt gagttttaca tcgataaaca agatgttgat gaaacaaatc
    18961 aagatttggc tgtagctaac atctcaaatg tatttattac agaacacgtg caacctgaaa
    19021 ttgatgcgta tcgtttttct actttagctt taggagcagg aaatactaaa gaagaaacaa
    19081 ttactgaaaa gaatgcttat acagccatta aagctgcaat tttaccagct cgtaaatttg
    19141 gcccacaaaa cctagtagcg tttgtatcaa caaccgtaat ggatgcatta gaacgttctt
    19201 cagaatttac tcgtaatatc actaatcaaa atgttgggca aacggcacta gaatctaggg
    19261 taacttctct tgatggtgtg ttattggtcg aagtttggga cgatactcgt ttcaaaacta
    19321 agttcgattt cactgatggc tacgctgctg ccgctgatgc acaagacatc aatatcttag
    19381 ttgtcgctaa acaagcagtt atccccgtgg ttaaagaaaa caccgtcttc ttgtttgcgc
    19441 caggcgaaca ttcacaaggt gatggctact tatatcaaaa ccgcttatac cacgactgct
    19501 ttatcaaaga acaacaaaaa gaaggtgtat ctgtctcttt ggccccaaaa gcttagcccc
    19561 atccggcgta actttgaata aaacaacagc tacgctaacg gtgggagcaa cagaaacatt
    19621 gtctgctact gtatcaccag aaacggcaac tgataaatca gtcaaattta cctctagcga
    19681 tgaaacaatt gcaacagtaa cgccagtgca aggaaaggtt actgctatta aagtaggagc
    19741 tacgacggtc acagcgacaa ccgtaaatgg taaaactgct acgtgtgaag tcacggtaac
    19801 cgcagcaagc gaaggatagt ttagtagcta tcctttttaa ttgaaaggag gcagttatga
    19861 gctatcttac gcatgaggaa tatctaaaat caggattcaa caaagtatca gtttcggagt
    19921 ttgatgactt agaaaaatgt gctgaacgtc agcttaatcg agtgacaggc gatttttaca
    19981 tgagacattc tttagctgat gacacgttca aatatcgagt ggataagttc aaaatcgcaa
    20041 tggctgtcca aattgaatat ttgaagtcag ttggagttac ttcgttatca gacttactaa
    20101 atgcttcacc ttcaagtgtc agcgttggtc gtatgcgtat tgaatcggga agcacgaatg
    20161 cagcaacagt tggcagaacg atggttgcaa cagaggctta taacgagttg atctatacag
    20221 gacttcttta caagggagtt gactatcgat gattccatta atgccaaaag aactttgtaa
    20281 ccagtcaatt actttgaggc tgctagatgg tcatgacaaa tggcaaaaac ctatcttttc
    20341 agacccaatc atgattaatc atatgatttt ccaacctcaa acagtgtaca gcggtagtaa
    20401 taataatcgg caagtggtag ccaacgctat cgcttttttg tttgctggag tatctgatcc
    20461 gatgccagtg attaataaaa atcatgttgg gtcaaagatt gactttgaag gtgagactta
    20521 cactatcact actatcgttg ataaccgtaa tccatacagt aacgaagtct actcgtatga
    20581 gttggaggtg ctgtaatgct ccatgttaag gttgaaaaaa atggcgtcga tcgtaagtta
    20641 tcagtgatga acatcaattc agcactgtac tatatgacat ctcaaatgca catggatatg
    20701 aatctatatg tgcctaaacg tcaaggaaat ttaagagaca aatcatttgt taacaagaac
    20761 cgaatcacat ataccgttcc ttacgctaaa cctcaattta gagggatcgt caatggtagt
    20821 agagttaaga attatacaac gccagggaca agccgacgtt gggacctcag agcaaaagcg
    20881 aatcacatgg atgattggcg tagagcgttt atcaaaggag gaaacttgta atggatttat
    20941 gggaacgatt atctgactcg atagattcta ttcaaggcct tccaatgcca tgctcgatgg
    21001 gatttctaaa cggtgaagat acactttgcg tttattctat gccgggtagc cgaacagtcg
    21061 aagaatactt tgacggtacg aaagagcgtg aaatgctcta tgaggtcgga tttaacacga
    21121 aagaccaaga aaaagccaat caaactcttt ggctcatatc aaatcattta gacgaactct
    21181 caactctgaa gtcagaagat gagagcttcg tctttttagg tatcgaaata agcgagacac
    21241 cttttgtaag cgaacaggac actcaaggga actcaactta tttattaggt atcaaaatca
    21301 ccattcatca attcaaaaat taggaggaaa tttaaatggc agaaaatagt aaagaatttt
    21361 tactaaactt taaaaacaaa ttggaaatcg atacttcagg aagtacagat ttagatcaaa
    21421 tcgcatcggc taaattcgca ccattagcag cagggatcac aactattact ccggctgcag
    21481 cggacactac agatgcatcc ccttactacg atggagaagg attcactgaa tccactgtaa
    21541 ctggtaaaaa tatcacgttc caagttgcgg gacaccgtgt atttggagat ccagctcaag
    21601 attatgtagc gtctaaattc ttgtcaatcg gagatgaatt acgcacgtta gcacaatgga
    21661 ctgatgccaa agggaataag gttcaagctg ttgttacatt gacatctatc gtgccatttg
    21721 gtggtgcagc taatgctaaa caaacgttca gcttcacaat ggcattcaac ggcaagccaa
    21781 aaacggtagc agcgggggag taattagccc cacaagcgta aagcttaata aaacaacgtt
    21841 atcgcttgtt gttggggcaa cagaaacctt aacagctacc gttttaccgg caaatgctac
    21901 aaataaaaac gttacatggt cagctgttga ttctacaatt gccacagttg atgcaaaagg
    21961 caaagtagtt gctgttaaag caggaactac ggaaattaca gttaacacag tagatggtaa
    22021 taagagtgca gaatgtgcat taactgtcac cgcactataa aactatttta gagcagatga
    22081 acgattggtt tatctgctct tatatttcag gaggaaaaca tggctattaa caatattatc
    22141 gacttagatg ctaaattatc gctaacaaaa tctatgaaaa tcgcagggaa agtatatgaa
    22201 gttcaaattt cagatgaaat tgacaaaact ttaaccgatt taacgactat tgatattcca
    22261 gatcaattaa aaaatatgac ttcaaaactt gaaaaaatgg atgaagatga taatggagct
    22321 aaagaattca aagaatttac tcaatctgaa atggatgaat taaaagataa agctgtaact
    22381 actctagatg tgattcttgg gagtggtgaa ggtaatcgcg tttataattt ttacaacaaa
    22441 agtacaaaag cccttttcac aattattgga ttgcttgaaa aagagttggg ggaagttgtt
    22501 tctgaacgta gtaaaacagc aaaaaaacat tacaaaaata atcgtaagaa gtgattagat
    22561 gtttgatcta acgagaaaac cagaaacaac agtgattatt tcaggtaatg agtatcaaat
    22621 tgatttgtct tttgacactg ttattcgatt ctatgaacta attgacgata aaaatttgga
    22681 gtctatagaa aaaataattc ttgggttcaa attgttttat atcgattcaa aaaaagcaga
    22741 agatacattc acttttgaag aaatgcaaca agctattaac gacatagttg actatattca
    22801 atcgaatcca tatggaagca ttggaagtga aggagaaccg actggtcagg attcaaatat
    22861 gaattattcg tattctcaag acgctggagc tatttattct tcgtttatgg cagattacaa
    22921 aatcgacttg ttaaatgagc aaggttcaat gcattatcta actttcaaag cgttgatgtc
    22981 tggtttaagc gaagacactc aatttcagcg gatactagcg attcggtcaa gaagcatagc
    23041 tggtttagaa ggagaagaac taaatagctt attagaactg aaaaattatt atgctcttga
    23101 ttctgaaaaa acagttaaca gtcttgatga tcaactaggc gatatgtttt ccatgttagc
    23161 agcacaagcc aaatcataag gaggtgagta tttgagcgca gatgcaacaa taaatattga
    23221 tgtgatgctg tcgaatttac ctaaatttaa aacagacgtt agttttgttg atgacgtatt
    23281 aacaaaattg ggaatgaata caggatcaaa aattgatgat tcatttaaag ccgagacagc
    23341 aaaagttaca acaattgcca aatcaacaaa aaaagatgtc gatcaaactt ttgataaacc
    23401 agttaagttc acaatcaagg ctgataattc ggatgctgaa aaagatgtca aagagacaaa
    23461 ggctttttta aaaggcatac cgaaaagcaa aataactgaa ctgaaggcgg ataatgacgg
    23521 agcatcactg aaaataaaag ctacaaaaga gggtataagc aagataccta acaggaaaga
    23581 aacaatactt aacgcagatg cttcacaagc aaaaacagaa acaaaagacc tcggtgatac
    23641 tgctgaaaaa accaaatcta agtttatcag cttgaaagat aaactatcta ttggtgcgat
    23701 tgctggtgct tcttcacaag cactgcagat tttaacaggt agtttttcag atttaatcgg
    23761 tgaaactgct caatcatctg acgcaatgga taaattcaaa tctacgatgc agtttgctgg
    23821 ctttacagaa aaagaaacaa aagaagctgc taaatttgta aaaaaatatg cagatgacac
    23881 agtatatgaa ttgtccgata tctcgaatac tacagcacaa ctagcagcaa atggtattgg
    23941 gaattatcaa gagttaacag aagcagcagg gaacttaaac gctgtagcag gtggtaattc
    24001 tgaaacattt aaatcagtag caatgatgct tacccaaacg gctggtgcag ggaaactaac
    24061 tacagaaaac tggaatcaat tggctgatgc cattcccggt gcatcaggta aaatgcaaga
    24121 agcgatgaaa aacaacggtg ctttcactgg aaactttcgt gatgcgatgg aagaaggtca
    24181 aatatcagct gaagaattta gtaaagcgat tgttgactta ggtatgactg atgtagctga
    24241 agaagctgca aagtctacta agactttcga gggcgcaatg ggtaacctcc aagcgaatat
    24301 cgttactaaa atgaatgaga tcgttgatgg catcggcaaa gataagctta cagatattat
    24361 cagcttcata tcagacaata ttactaaact atttgattct attttgaaag gcatagatta
    24421 tttaaacgat aatcaggata aattatcgag tatctttgat aatttgaaaa agatttctaa
    24481 aatatttttc ggtgctgcat gggatgcggt tcttgatatt ataaaaaaac tcactggtaa
    24541 ttttagttta ctttcaaaag aaactggcaa aacgaaagac cccttacaac gatttgatga
    24601 tgtcttaagt gctttgtcga aacatgaaaa gggaatagca cttctaggta aagcattagc
    24661 tactttattt attgctaaaa aaatcgttgg tgtggtctcg gcttttggga aactgcttga
    24721 tatgttcggc ggaaaagcat tactttctag tccaattttt cttatacctg cagcaattgc
    24781 aggaattgga tttgcgtttt ataaagcata taagacaagc aagccatttc gtgagtttat
    24841 agatgggatt gtcgatgcag taaaaaactt tgtagaggta tcattaaaga aaatcaaatc
    24901 attttttaaa aatgttacag aaggatttgt tgaatttaag gattcagtta cggataaagt
    24961 gactagtatc aaaaaaagca tcaagaaagt ttttaactcc gttattgatt tttttaaaga
    25021 ggattggaaa gaagtattaa cattcattct aaatccaatt gctggtgtag ttgctctatt
    25081 gtacaaacac aataagaagt ttaaaaagtg ggtagatgat ctacttgata ctattaaaga
    25141 tggattgaaa gcttttaaaa agaaagttct tgatccacca tacgatgcaa ttacagattt
    25201 gattgataaa atagctaaaa ctttttcaaa atggatgggc aaaatagaaa aagctcttga
    25261 aaaagcgggt aagaaacttg ggaaagctac agaggtaatt ttttggctta tctatgggcc
    25321 aatacggtta ttaggtagaa aaataaaaaa aggtttcgat gaagttgctg aatggataga
    25381 agataaactt gaaaaagcag ggaaagcagt tgggaaggtt gtcgataaaa tctcttcaac
    25441 aataaaaaaa atctttaatt ctttatcaaa atcagtaaaa aaatcaatgg aattatttac
    25501 tgaatatgtt attgagcctg tggattcagt tcgaaaaaaa gtcgttaaaa caattagtaa
    25561 cttagtagat aaagtagtcg atttctttgt tgatttagtc aatattacaa agcacaaatg
    25621 gagagaaata aaatcctcga tggaaaaacc ggttgacgat gctaaagaaa atgtctctaa
    25681 aaccatcagc aaattaaaag aaaaaatagc tgaaattttt gaccgtatca aatcaacaac
    25741 taaaagcgca tggaatacaa ttaagcgcta cacagtggaa ccaatagagg acgcctacaa
    25801 aaaagtagtt gaaaaagtag gagagattta ttctggtgtg acaaaatatt ggaatgattt
    25861 aaaggacaaa accaaagaaa aattcgatga aatcgttgat tatgttaaaa aagttcctgg
    25921 tcgaattggt gatgctttta aagatggaaa agaagcaatc ggcgatggta ttaaagctgc
    25981 agcgcaatac atggttgata ttttaggtac gggagtcaat ggcgtaatta aaggtatcaa
    26041 ctgggtactg gaaaaagtta atgcaccaga ttctgttcga gttgataaat gggatgttcc
    26101 taaatttgct acaggtggta ttcaccaagg tggattgatg cttgttaacg atggcgaagg
    26161 agaggaattg gttcgacatc ctgatggaag aatggaaatt ccaaaaggga aaaatgtttt
    26221 gatgcatgcg gaagctggta ctcaagtatt gaatcatagc caaacaaaat cgtttgctga
    26281 agcttttggt attccaatgt acgcaaaagg aaacgtaagc aatctaggtg atttcttcaa
    26341 gtctgcgtgg aatggaataa aagacattgg ttctgatatt ttagatgcag ttcaacatcc
    26401 agtggagttt gttaaaaaag ctatttcgga acatgtgaat tttgatgcta ctcatccagt
    26461 atttgatatt gctacaggcg gtgtcaagaa agtaacaaat ggcgttatgg attggatcaa
    26521 agataaaata gcaagttttg gttctattgg tggaagtttt gacggggcga tggctgacaa
    26581 tgtctataaa tatttagtag atattgcaaa tcaaacagta agtaaatttg gcatgagtgg
    26641 tatcacttcg ggttatcgac caggagaccc ttattaccac ggaaaacacc aagcgattga
    26701 tattgcttat cctgctggaa tgaatggttc aagtaagtat ttcgatcctg cgaactgggt
    26761 gtttgaacat tttgctgata aggttggcta tgttattact caaggtaaag ttcgagatag
    26821 gacaggtcaa tcagggcaac ctgctacagg aaattgggag acatggccag ataacgatca
    26881 ctacgaccat ttgcatatta caggtaagct tggctctggt gatatttata aagcaggcga
    26941 aggtggcgga aaaggttcgc ctacaggttc tggcgttgca aggtggacta gccaattaaa
    27001 agaagccttg cgaatgaatg gattaccaac cactgcttcg tatgtcaatg cctggttaag
    27061 gcaaatcgag actgaatcag gaggcaatga gcgtgctgtt cagcctggaa tagacccaga
    27121 tggtgatggc tctggtccag caatgggact aatacaagct aagaaaggaa catttttagc
    27181 aaatgcgttt cctggccacg gaaatatttt taatggtttt gatagcatgc ttgctggtat
    27241 tagatatgca ctgaaaagat atggtcctga tatgctcgca gtcattggtc atggtcatgg
    27301 ttactcaaat gggggagaag tatttggacc agagttagct atgtttggtg aagaccctgc
    27361 ttatccatat gaaatcaaca tcaatcctgc aaaaccaagt gcagatatgc tgattcaaaa
    27421 agcgattgtt gcaagagata gatacaaacc agccgtacaa acaaatcaag caggttattc
    27481 aaatcaacat ccaagtggcg gaaatgagca caaaacgatg agtaaaaaag atattgaaac
    27541 aatcgttcaa gcgttaaatg aaagaccagt gcgtgtcgaa agtattctag acggtaagaa
    27601 agtcagcaaa agtgtagatg aatatactgg ttcatcatta gcaagaaaac tatatacgag
    27661 aggaaagaat ttcaatggat gataaaacat cagtatttct ccaatttagt acaggtaaat
    27721 ttgacttact agcaaattac cgaataaaaa tcattgatat aaaaattggg atgccagtac
    27781 ctaaaaatga attttcttct tatgcaggtt cagtaggaaa aaagctgctg acacactcat
    27841 ttgattcttt tcctattact tttgaatttg attattttgc agacaatcta aatgatctta
    27901 ttttgactga aacagaattg agagaactat ttaataaaga agctgaatac tactttatct
    27961 atacgaaaga acctggtaaa agatacccag tgattgttga gagtatgact gtaaccaaaa
    28021 aggcatattt taaaggaaat tgtgttgtat cattttccgc ctataaagga tattctgaat
    28081 cgatggcgac gactttatct gatttcagtt tggatgagga ttggcagttt tctcaaggtc
    28141 tagtttctga agattttagt tatacacaca atactagttt ctttaagatt tttaatgctg
    28201 gcagttttga aattgatccg agagagtcag atttacgtat taccctcgaa ggagaatcag
    28261 aaggaaatgt gactattttc aataagacca caggcgatcg tttcatttat tatccttctc
    28321 tctcaactaa tttagggcag acgttagttt tggatggtgt atacccaaaa ttgaatggtg
    28381 taagttgtgg tattgataca aaccatggac taatcacttt agctgaaggg gtcaatgaaa
    28441 tcgaaattca aaatattact agagtgaaat cttcttggga tttccgtttc ttgtataagt
    28501 aggtgatact ttgaaaaaca tattaatacg taattatgaa gaaacgaaag aggaaatcct
    28561 tattaactac gataaggatt ctttttctgt ctcgtggcaa caaaatgaaa cgtgggagtt
    28621 atctgtgact gtaccagaaa caaaaaggaa tcaaataact tttgatttaa ttgactatga
    28681 aaactatgtt gtttttgatg gtcagcagta ttcaatcaag cagatgagac catatgcttc
    28741 tggtagccaa atctataaag atgtagtagc aactcatgtt tattacacta ttcaagatgg
    28801 atggcaatat gacactatat ctggaacaaa atcaatcaat gatcttttga ctcatatttt
    28861 taaagctgga aaccgaggat ttagttggga agttgtagat cccaacaatg tatttttaaa
    28921 gaaagaacag gagaattttg gaaacgataa ttatttaaat ctcatcaatg aaattttgga
    28981 agattacggt gctgttgtga taccaaacaa taagcattta gtattttatc ccatttcaga
    29041 atatggaaat ataactgagc agcaaatccg atataaatat aatacagacg aagtgtcttt
    29101 tgatattgat acttatgccc tgaaaacaca aatcaaaggc tatggtaagt tgaaagaaaa
    29161 tgcaaacaca gacaatttaa aagatagtga ttatgtcttt cctcccgtaa cttatacgag
    29221 tgctgaatca gaaaaatggg gcattagaat acaagatcca gtggaagacg agcgttacac
    29281 aattcaaaat aatatgctcg tgtatctgaa acaacagtta catgattatc cagatatttc
    29341 tggttcggtg acattgaaat gggctgtatc tcttaacaaa ggggataagg ttctttttgt
    29401 ttatgaacct ttgaatataa gtacctacat tcaagttgtg ggaattactg attatccagc
    29461 tatccctaat aaagcgccag agattgtatt atcaaatacc aagaaaacaa tcacttcaat
    29521 actggcaaat cttgctaaga aagggctgat gtaaatgggg ttaattaaat tattatcaaa
    29581 caatattgct ttgaagtgga aagaaacatt taaccaaaat gttgattatc tgaacaatct
    29641 tgagaagaaa ctgtctgatc aagacaaatc aacaaacagt cgtatagata atctcgtgct
    29701 tcattcaggc ggtgattctc ctaacgaagt agtggatgct cgaataaatg ctgaaggtac
    29761 gatctatcca acgctttatt cgagattatt agcattggat aaccttttca atttgaatta
    29821 tacagaatta aagacaagac aagataatca acaaggtcag ctaaatcaac taaatgtttc
    29881 tgttggaact cttatgggag catacggcga aacgctcgat ttatatgttg ctaaaacagg
    29941 aagtgatcaa agtggggatg gtacagaaaa aaatccattt cttacaattc aagctgctgt
    30001 aaatcaaata ccgttattaa ctagttcacg agtaactatt tggattggtg atggcgtata
    30061 ccttgaagat gtcgtgattc gcaatctcaa agcagttagc attactctgc gcagtcgcca
    30121 aagtgtaaca gatgcaacgt ctgacttaag tgttaaagtt cgctccattt catttattag
    30181 ctctttaggt taccaacaag ttaatggaat cgagtttgtc gatcaggcaa atatttccgg
    30241 tgcggcatat attgacgggg atataaaatg tgcaatctac tcagagcaat caagttatct
    30301 aggagtttgg aattgtcgtt tcgctgaaaa tacctttggc aaagggaata gatgtctttt
    30361 cgccatagga gcttcaaaaa ttggtacaag taataatttc tatcaaaatc agaactgtat
    30421 tgctgaagct agaaatttag ctgatatcaa tattgattca aatgatcgcg gtagtggaaa
    30481 tgactatggt gtaattgccg ataacggaac tgctcgagtt aaagtagctg gatcaaaagt
    30541 gaaggctaat agaattgctg aagtaagaaa tcaagggaat gtcgttacag gtaaaatcat
    30601 tcgccaaatc actaacgatg atattagcga tcgtgacaat attacaaacg ttaacggtac
    30661 tattaaacgt gaaggagaca cagtcacaat agcaatcaag tatgaatgca ataattatcc
    30721 ttctgatgct tctaacacaa gaaatgttat tttggttccc gctggatttc aacgtgatca
    30781 aagttatcct gcttaccatc cgctggcttt atatcgcaat gaaacccaac ctgctggcgc
    30841 aagggcaggc ttgacacaag ctagtcgcgt agtcgcatac tcaggaaatg gatcatctta
    30901 tatttcaggt acatgggtaa cgaatgaccc aataccaatt atttaagaaa ggaggatata
    30961 gatgtttaaa attaatgaat cgattattgt gattcaagca gaagccacta gtccaaacag
    31021 gacgaatgtt gttttttggt cgcatgatcg gggaacagct aagcttcgaa tgaagttagt
    31081 tcggaaaaac ggcattcctc aaagtctccc agaaggaact acggttccga ttcgcttgat
    31141 gttcaaatct gcaacggcag aaggtggtta tggtaaacat gactatctag ctacggtaga
    31201 agatcctgtg aatgggattg tttctattgt gttagaggat aatatactgg gatacgtagg
    31261 caccgtagaa ggtagcgtat atattgattt cccaaacgac cgctcgttag atacagctgg
    31321 tcgttttact ttttatatca aacgtagtcc aatcgatgat agtacgccag aactagaaaa
    31381 ttattatttc aatggtttta gccagacaat tgataaaatc gaaaaaattc tagctgatgg
    31441 aaagcaagag attgatcaga aaattacgga atctgaaacg cagattgatg cgaaactgaa
    31501 agacacaagc aacaaaatca cgaaagccaa tcaagatgtc gcaactatca atacgaatat
    31561 tgataaagca aatgatcgta ttgatcaaac gaaccagcaa ataagtgatc tcggaaagct
    31621 gaaaaagatg tactccaaca gcatcgattt cgggaactat gattatagtg gaaatccgaa
    31681 tttgatggct aatataaacg ctgatagctt ctcgcaaggt agtggtgcct tatctgttgt
    31741 agatgatggt gacgaggtag tgattacgct tgatccaaac cataaattag aaatattcaa
    31801 agcgaaaagt caaccagctc tggtagtggg gaaaacatat accatgagtg tagaaattat
    31861 gttagaagat gatttcactg gagaccctag taatatatct ctaagatata ttaaaatgcc
    31921 cagctggtta acagtactat atacgcctta tacattaact aatactaagg gtgtatggca
    31981 aaaactaact gttaccgcta aaatgactac tgcgattgat aacgctgaaa gctggtttat
    32041 aatgctacaa aataaagacg ctaataacag cctatccggc aaactacgtt tgagacacgc
    32101 taaactcgaa gaaggctcaa cagctacacc attccaacct aacttattag cagaacctta
    32161 caacatgtgt cgcgaatatc ctaacgaaaa tattgccgat cctaaagtta agttcccaat
    32221 cgaatctggc gaccaccaaa tatatcaagg ctacacagaa gaagagctta tgataggtca
    32281 aacgtatact atcacgctta aaggaacaaa acccgcaagt caaacctttg tagcgtataa
    32341 tcattggact gctcgtttag gagaactaaa gccggttgat gggttgacag acgtatggtc
    32401 tctaacattc acaccaacga atgttgtggc gatgcctaaa cttttccgtg tttatcagta
    32461 tccacgatca acagtaggcg catgccaaat tgactggctc aagattgaaa aaggcaacac
    32521 ccgaacgccc aatattagtc agtttaaata cttcggtgaa ggattgaaag acagcaacaa
    32581 tccgaatgat tacagttggg atgtcacacc tgaatatact gaaaaaggct tgaataatgc
    32641 tgttaatgtg tatgatcctc agagagttga aggtttgaaa aactttgccg atggtattca
    32701 aatagcagga gataaagtga tcagtgaaaa tgattgcact gtttatacat taactaaaga
    32761 caacagtcaa tcgtttatcg atgggtatgc aacatttatt aaacacggaa aagaagttgt
    32821 tgtaaatggt acagtaaagt tcaaaaaagc ttatgctttt ggtgtaccac ttgatgatga
    32881 agtcccagat gaatttattg caagaattgt tcatggcatg cttacaggtc agtcaggaac
    32941 aaatagtgta tcaaaagcga tgtacgtgca aaaagactta ggaaaaatta tgacgaatag
    33001 cgaatttgcc gcaaatgaat ggtttacatt ccacggtaat tattgggtag gagtgaaata
    33061 aatgaaaaac atttggaaat acggacgtac tggtggagag tacgcaggaa aagtattgga
    33121 cgacatgctt gtatccgttc cttacacgga tcagccaccg ctcgaaggat tacgtgctga
    33181 tggcgaaccg ctaacgattg ctgatcagat gtttgatcct aaactgaacc aatggattgt
    33241 tttagcgaac acactggatc acaacgattt aaacaatctc aaagcgatgt acgaggcact
    33301 ggaacacgag aacggcgatt taaaacagct caacgccaaa ctcatgctaa gcgatgtagc
    33361 aattaaacag gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc
    33421 aaaaatgatg cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact
    33481 aaatccaact ttaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt
    33541 atgattgggg ctgttttact gacgatcaag ttcgactatt cgttccacta tgcattacag
    33601 acgaagaagc agataaaatc attagcaaag aagagagcgc atcttaagtg atgcgttttt
    33661 ttgttggaaa gttggtggaa catgaaagaa gaagcgctcc aagacgttgt ggagagatta
    33721 gtaagaattg aaacaaaatt agacaactac gaatcactta gagaaaaggc tgatagtgca
    33781 aaagatttag cagataaagc ctattcagta gcactaaaca atgcagaaga catcaaggaa
    33841 atgaagaaca ataataaatg ggcttggggc tatatgattg gcttaggcat tacgatcatt
    33901 ggctatttct tgactaaatt gtaaaggagg tgagaagaaa tgattttacc agataagtat
    33961 tatcaagtca ttaaatggac agttttaaca gtattgccag ctgcatctgc tttagtagcc
    34021 atgttaggga aagcgtatgg atggaatgga acagatatga tagtgttgac tatcaatgca
    34081 gtagcaacat ttttaggcgt tatcactggt gtgtcggctt ataatttgaa aaaataggag
    34141 gaaacaaatg aaaaagaaaa ttactattac tgcgatgagc ctattaacgg ctcttttttt
    34201 attgccaatt aatgggtttg cctatactat caacaatgaa tttaatttgg gcgcaaatga
    34261 aggtagctca caaatagcaa ataatcagta cattttactg catgaaacgg ctaatgaaac
    34321 agcaacagga cgcaatgaag cgcagtatat gcaacgttca tggactagcg cttatactgc
    34381 ttatattgtg ggagacggcg gaattgttta tcaagtcggt caacctggtt atgtacagta
    34441 cggtgctggt tcgtatgcta atgccaacag tcctgtgcag attgagttac aacacacaca
    34501 tgataaagca acgtttgaga aaaactacaa ggcatacgtt gaattggcta gagattcagc
    34561 aatgaaatat ggtattccat taacattaga cacgccttat aaccaaccag gaatcaaatc
    34621 acatttatgg gtaacacaaa atatctgggg agatcatact gatccatacg gttatctttc
    34681 tgaaatgggc gtaagtaaag aaaaattagc ctatgatttg gctcatggat ttaccgatga
    34741 aaatccgaca acttcagatg ataaaccagt cattgatcca actagagcag gtgcagcaaa
    34801 tcctacactg acagatggaa caaattacgc ccacattgat cagttcggag aaatcgaaaa
    34861 tgcgaacttg catgtggctg gatggcacat tgctaactat aaatacgagt atattttcat
    34921 tatggactac aatactggga aagaattagc tcgagtaaaa gctgatggaa tatatagacc
    34981 agatgtaaat caagcttata atacttctgg aaacgttggc tatcatgtat ctttcaatat
    35041 gcgtaatttt cctaataaga aagtctatgt catgatgcgg gcaacgaatg atccagaagg
    35101 gaacactaaa ggcggagcac aagattttca tgacaaacgt tggtatttaa atattccgaa
    35161 acgataaaaa atagcccctc ga
//
LOCUS       DAPBAB010000023        34788 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPBAB010000023 DAPBAB010000000
VERSION     DAPBAB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.023"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(468..3617)
                     /locus_tag="RD444_001393"
     CDS             complement(468..3617)
                     /locus_tag="RD444_001393"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDT7548776.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3630..4847)
                     /locus_tag="RD444_001394"
     CDS             complement(3630..4847)
                     /locus_tag="RD444_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDT7548777.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(4837..6432)
                     /locus_tag="RD444_001395"
     CDS             complement(4837..6432)
                     /locus_tag="RD444_001395"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDT7548778.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(6942..7724)
                     /locus_tag="RD444_001396"
     CDS             complement(6942..7724)
                     /locus_tag="RD444_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548779.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(7789..8625)
                     /locus_tag="RD444_001397"
     CDS             complement(7789..8625)
                     /locus_tag="RD444_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7548780.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8643..10052)
                     /locus_tag="RD444_001398"
     CDS             complement(8643..10052)
                     /locus_tag="RD444_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7548781.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10066..11889)
                     /locus_tag="RD444_001399"
     CDS             complement(10066..11889)
                     /locus_tag="RD444_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7548782.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12732..13058)
                     /locus_tag="RD444_001400"
     CDS             complement(12732..13058)
                     /locus_tag="RD444_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548783.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13104..13466)
                     /locus_tag="RD444_001401"
     CDS             complement(13104..13466)
                     /locus_tag="RD444_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDT7548784.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLEMSMY"
     gene            complement(13503..13718)
                     /locus_tag="RD444_001402"
     CDS             complement(13503..13718)
                     /locus_tag="RD444_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7548785.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            13845..14180
                     /locus_tag="RD444_001403"
     CDS             13845..14180
                     /locus_tag="RD444_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7548786.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14256..16253)
                     /locus_tag="RD444_001404"
     CDS             complement(14256..16253)
                     /locus_tag="RD444_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548787.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16351..16626)
                     /locus_tag="RD444_001405"
     CDS             complement(16351..16626)
                     /locus_tag="RD444_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548788.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            16859..17593
                     /locus_tag="RD444_001406"
     CDS             16859..17593
                     /locus_tag="RD444_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7548789.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINVIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17632..18774
                     /locus_tag="RD444_001407"
     CDS             17632..18774
                     /locus_tag="RD444_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7548790.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(18869..20260)
                     /gene="sufB"
                     /locus_tag="RD444_001408"
     CDS             complement(18869..20260)
                     /gene="sufB"
                     /locus_tag="RD444_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDT7548791.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSASSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20265..20735)
                     /locus_tag="RD444_001409"
     CDS             complement(20265..20735)
                     /locus_tag="RD444_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDT7548792.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVQDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMKNDGTAAFHHYHKEEE"
     gene            complement(20722..21957)
                     /locus_tag="RD444_001410"
     CDS             complement(20722..21957)
                     /locus_tag="RD444_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7548793.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPFVYLDNAATTQKPKQVLQSLAH
                     YYEHDNANVHRGVHTLAERATHEYEQAREKVRAFIHAEETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIDLTEEGFLDMEDAAKKI
                     TNKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIAFGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTHDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(21954..23240)
                     /gene="sufD"
                     /locus_tag="RD444_001411"
     CDS             complement(21954..23240)
                     /gene="sufD"
                     /locus_tag="RD444_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDT7548794.1"
                     /translation="MKEKNWLDYLNAVNDFSLSKGEPDWMRTFRQDALTKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMDEDQLTALHVAFMNSGIFLYVPKNIVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKVPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23253..24023)
                     /gene="sufC"
                     /locus_tag="RD444_001412"
     CDS             complement(23253..24023)
                     /gene="sufC"
                     /locus_tag="RD444_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDT7548795.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFNGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEA"
     gene            complement(24220..25068)
                     /locus_tag="RD444_001413"
     CDS             complement(24220..25068)
                     /locus_tag="RD444_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDT7548796.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIALEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIAENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25092..25778)
                     /locus_tag="RD444_001414"
     CDS             complement(25092..25778)
                     /locus_tag="RD444_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548797.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(25771..26805)
                     /locus_tag="RD444_001415"
     CDS             complement(25771..26805)
                     /locus_tag="RD444_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7548798.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(26868..27124)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(27200..27538)
                     /locus_tag="RD444_001416"
     CDS             complement(27200..27538)
                     /locus_tag="RD444_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDT7548799.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27540..27896)
                     /locus_tag="RD444_001417"
     CDS             complement(27540..27896)
                     /locus_tag="RD444_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDT7548800.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(27902..29089)
                     /locus_tag="RD444_001418"
     CDS             complement(27902..29089)
                     /locus_tag="RD444_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7548801.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPARGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29280..30125)
                     /locus_tag="RD444_001419"
     CDS             complement(29280..30125)
                     /locus_tag="RD444_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7548802.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30359..31324
                     /locus_tag="RD444_001420"
     CDS             30359..31324
                     /locus_tag="RD444_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDT7548803.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            31824..32867
                     /locus_tag="RD444_001421"
     CDS             31824..32867
                     /locus_tag="RD444_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDT7548804.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(32929..33393)
                     /locus_tag="RD444_001422"
     CDS             complement(32929..33393)
                     /locus_tag="RD444_001422"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDT7548805.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            33638..34585
                     /locus_tag="RD444_001423"
     CDS             33638..34585
                     /locus_tag="RD444_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7548806.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT        10054 a         7205 c         5984 g        11545 t
ORIGIN      
        1 taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga ttccatttaa
       61 tttttatctc attttttcct actagttagt atcaaatact ctcctatttt ctcaataaac
      121 tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattatagc aaaaaatcac
      181 tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa aacaaaagaa
      241 aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac ggtcatataa
      301 caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat agctgtctta
      361 aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt ctacacttcc
      421 acttgactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta cctcaattca
      481 ttctcaagtg gaatcaactc atcatctaac attttacgcc atgctcgctt catttcttgt
      541 gggtatttaa atggcttcgc ttctggatgt ttggctttat atccttcgta atccttactg
      601 ttgataattg ctttcatatt aggaattgga tcttcacctg gtagatattg cagattagaa
      661 gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat caaatcatca
      721 ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact atctaaagtc
      781 cccacatcaa atgcatttaa aatttgtcgg taaacttctt tgactccttc tgttttattc
      841 aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc agcataagtt
      901 cctagtaatt gatcaatata agttgaatca atatcataca acttagtggt attttcacta
      961 taaaattcga tcgttgaaaa atcttctcct tctttttctg gttcttcctc tattaatgaa
     1021 cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg attgttcaaa
     1081 gttggagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga ttcataagca
     1141 cgattcaatt cttgaaatgc tttgacgtac tcgatacgga tttcaagtgg cgtatgttca
     1201 tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgttt gaatcttttt
     1261 ttggattcct gataacttgg ataaatcaaa cctgattctt cttttgcttt tgaatacagt
     1321 tttgtcgcat cctcgacatt cttctccatt gtcgctggct tacgaaacgt cacaatcaat
     1381 ccttttgtct tatcaggata cgtacgattc gtacgtgaaa aagcttggat caatcctgca
     1441 tactctagat tacgatccac aaatagggtt tgaatcgtag gggcatcaaa acctgttaat
     1501 agacgatcca ctacaatcgc taaatcgatt tgtttcccaa attgtttgaa ttccgctctt
     1561 tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc taatgaccag
     1621 gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc ttgttgggca
     1681 cttgaatctc ggctattttc ttctaatgaa taggtaatag cgattcgtgg aaaatcagga
     1741 tcctccatcg ttcgtccttc acgaatgagc tgattgggaa actcttttgt taaccaatca
     1801 ggctctttcg tcatctcttt gatggcccga taatactttt tggccatgtc aatggaactc
     1861 gtggttaaga ttgcagattt cgttggcctg ccatttcgaa aatcaaattt tgtataggca
     1921 ttatctggac ggaacatttt atgaatgact ttttggatat gttcatctgc ttcaaaaaca
     1981 gctgggtcca aataactttc ttttttgaca ggttccattt gatcaatcat acgattgatc
     2041 tgttctgatg aatactctgc atatgtttct acttctttta atttccgata gattttattc
     2101 tctagtgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa gacagagcca
     2161 tcttctagcg cattcttaat ggtataggta tgcaagacct ctccatactg atcatgggtt
     2221 gtacgtgcca attgcccttt ggcttgtttc cgattctcag gaaaaatcgg tgtacctgta
     2281 aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt ttcagcactt
     2341 aacgctcgat gacactcatc tactacaaac acgatatgtt ggcccattaa tttttggaag
     2401 cgattggttc cttttttctc ttcttgtttc tttgcatatt tcaacgccgc atctagtttt
     2461 tgacgggttg taatgattac aacatttgca ttggcatctg ccaacagggt ttcacttaat
     2521 tctttggcac ttcccgttcc aacgatcaag ctattcgctt tggcgttccc ggaagaaatc
     2581 ccagtattaa attccgaagc aaattttgta aactctgtgg tggtttgatt atctaaatct
     2641 ttgcggtcca ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa aagcttagta
     2701 gatacaaaac tagtcaacgt ctttcctgaa ccagtcgcat gccacacata ccctgattga
     2761 tgtttatttg cggcaataaa caaggcttga atcgcatgta cttgataagg atgtaacacc
     2821 atgagggttt tattgtcttg atcttcactg acgattgtat aatccgcaat caaacgatga
     2881 gcatccggga tatttaacac ttgtttacaa aactcatata agttttctac tttttcattg
     2941 tctttcgttc gccaactaaa taaaaactta gggtgcaagt ctttcggtaa ggcatttgca
     3001 aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga aaagatattg
     3061 ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaatgcctg ataaacacca
     3121 tcttttgcag ttacttgttt cagctcgatt tggacaatcg gtaatccatt gattaataag
     3181 gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc aatttgatgg
     3241 accacttcat aagtagagat tcctccacca atgtcttgat tggaataaag gataagtgag
     3301 acagaaccca attgcgaatc ttcccgttca atcgtgatcc gagccatccc attttcacct
     3361 tttaaccatt tcgcagcatc aaatggggtt ttcgttcgca gcagtaattc cgttttgatc
     3421 gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt atttgagata
     3481 attttttggc gtaaattgac ccaaaggtct tcttctgatt ttagatccgg acgatacgtc
     3541 cactgattat gcccttctcc aagtacacga atcaactgtt cttcgacttc tgcttcctca
     3601 cggtgactaa ttttcattct tcttccctcc tagacaaaca tcttttgtaa gaagcttttt
     3661 ttcgtttctt ttagtaaatc taacttacgt tgatgaagag cgatcgtatc atctagctgt
     3721 ttgaagaaag taccgatttt ttgttgttca tcctttacag gaaccttaac actgatattc
     3781 tcaatatcag aactgttata agaaggcaat gcccctactt gggagtatct tggcaaattt
     3841 atagtttcaa acaatatttt accaaaatag atatcccaag tattattaaa taaataagcc
     3901 atggtattgt tatctattaa gaaagggctt gtgaccaaac gttttctatt aagcattatt
     3961 gctgcgccaa ctttagcaaa tataatcgct ggcacatctt ttattggctt ccatttttta
     4021 cgctcaattt gctcattaga tacataatga tttgcatttc tcatttcata ctcattacca
     4081 atgttattca tgtcggaaac tttaaaaaat ggagtacctt cagaaccacc ttgctcggca
     4141 tcaggaaatc caattcccga ttgagtttta cctaattctc ccaacttacg ctcttcccaa
     4201 tcttccgtaa aaccaggaaa ccggatttct ggcacttttg ctccgttttc agggaacatt
     4261 ttttgtaaga atcctttttt cgtttccttc agcaaatcta acttacgctg atgaagagcg
     4321 atcgtgtcgt caagttgttt gaagaaggtg ccgatttttt gttgttcttc ctcactagga
     4381 aacattgcta ctatattttt aaactttttt agagacagaa catgttgacc tgttccctct
     4441 gcttgaatag ataattgtcg ttggattcca ttgttaatac gtcttaagaa gaagtcggaa
     4501 tcagcagatt ttaaagtaaa tacaggataa aaatagctta taatccctct atcaataatg
     4561 tcattacggt taaacacaaa cacatcatta tcacttcgac tacggaacgt gaaatatcct
     4621 ctaggtagta caaagtaacc aatattattt tctgtagtta cttgccgatt tgcaaaatag
     4681 tcttcttgca aaactatccc tttttgttga gatgaagtta gcataggaaa ttgattttgt
     4741 acacttgtct tttcaacata atctacaacg aagtccccca acttcctctg ttcccaatca
     4801 tctgtaaatc ctgggaaacg gatttctggt tgtgtatcat tgctcatgtt tgaacacctc
     4861 cagcgcacct tgtaaccacg cttcattttc aggtgttgtt tgcagggaag agatagcttc
     4921 taaaagtttt ttttctagca cttgctgttc ttgtcgtatc ttcttgatgt catttccgac
     4981 atgaaccata tcaattggtt cttcttcttc gaaagtatct acatagcgtg ggatatttaa
     5041 gttatagtca ttttctttga tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc
     5101 ttttctttca gcgtaggttt cgaggatttt ttgaatgttt tcttccgata gtttattttg
     5161 atttttccct ttaacaaatt cacgactagc atcaatgaat aatacatctc gagtttgacg
     5221 attcttcttc aacacaatca ccgtcgttgg aatcgatgtc ccaaagaaca agttcgcagg
     5281 cataccgatc acagcataaa tactgccatc ttctagtaat ttttgacgga tgaccccttc
     5341 tgcagcgcca cggaataaaa ctccgtgtgg taagacgatg gccatggtac ctgtttcctt
     5401 caaatgatag aacccatgaa gaagaaacgc aaaatctgct ttggattttg gcgctaattt
     5461 cccataacga ttaaaccgag aatcatctaa gaaagtcgta tctgctgacc aattagccga
     5521 ataaggtggg ttcattacta ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt
     5581 attcaacgta tccccattgc ggagattcat ttcttcggcg tcgacaccat gtaaaatcaa
     5641 gttcattttt gctagattgt atgtcgttgt atttagttct tgcccatgat atttaacgtt
     5701 atctgggtgt gttaagtagt tacgtacgtt taacatcaat gaacctgaac ccatcgttgg
     5761 atcaaaaaca ctaaagaatc gtcgttcctt ttgatcgagc gttacgattt gcgccatcat
     5821 atcagaaacc atatgaggtg tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa
     5881 ttgactgatc agaaactcat acgcatcccc gatcacgtcc ccatcgtgtt ctaaaacatc
     5941 tacatcattc aatttcttga tgacttcggt aatcgtcaca tttcgttgtt gctcatctgt
     6001 tcctagtttt tttgattgga gatccacatc atcaaataac ccattaaatt gattgtaggt
     6061 agatgccaat tgcacaaagg ctttgtttag atcgttcaat tggaacgttg cttgttttgc
     6121 ttgatccgct aagacgttga aaagatactc tggtgcaatt gcatatccca aaatatcaac
     6181 gattgtggca atcagatctt cttttgattc ttcatctgaa agtaattcac gatacaacat
     6241 tgtttgtttg cttactgtgt catattcctc taaggattca tctgctaaaa gaaccacttg
     6301 ttccaacaat cgatcggata aatatttata gaaaatcagt cccaataaat aatttttata
     6361 ttctgacgca tccattttac tacggagatt atctgccgca ctaaataatt tttgatttaa
     6421 ttctgcaccc aaaaagatac actcctatct cttatttaaa gttgctatat accctcttat
     6481 tttagcatac ccccttttat aaaaagcaaa aaaaaaaaga aagaaactta ttcttgctaa
     6541 taaactacgc gaaaggagtc taacgttgtt tgatataact tagactgatc gttaacttat
     6601 tttttatatt tctacatagt tatctttaga accatttcag atacatatga aacaattaca
     6661 tatgattttt tcataaaaaa acagtactgt ctagtagtat aaaatataca aatttcttaa
     6721 aaattttttc actaaaatca ctacctgaaa cagtctaaaa cacttatata tcaacgttta
     6781 atgaaactat ctccgtttca tttcagatac aaataaatat agtcgggctc caaatccgaa
     6841 aaaaaatacc cccagtagct tatcctaagc tactggagat gtataagtac ggtcatataa
     6901 caacggggta gggtaaataa atgaaaaaga tggaagaatc attattcaat aactttttta
     6961 aatacttcgt tgtaatcagt tacctgtccg ttcttaaata ccacagaata atcttttttc
     7021 ccattttcaa gtaaccgaag tggaatgtct ttaccaactt gctctgtcac tgcattactg
     7081 atacctgtta gattagctaa taaatctgat aaagattgag catctgtaga aatctctgta
     7141 tcagtattta tcgtgattcc tttatcttca tttacaagct tccaagtagg tggtatagct
     7201 gtgtatgttt cgatgaatga atcaattctt tcatctatcg tcttcccagt atctacttct
     7261 gtctcattac tcttagatga atcgattctt tcatccatcg tctgctccgt aactgcttct
     7321 gtctcattat tctcaccgga aactctcttc acacccaaac ttatatattg tccatcttta
     7381 gtgtgcatag ttaaaattcc cacgctttta ctaataaatt cactataacc agttctttca
     7441 atagagactt caatgtattt cacactatca tactcaaaag aaaaatagac atgtaaaagc
     7501 attttgttgt ttacatctac ataataatcc tttgactcta cttgagacct tcctatacgg
     7561 aatacagctt tactgtctga tatctctata ccagcatcct tattattaat ctgccaatct
     7621 ccaagtagct cttctggcaa gctagtagtt tcttgtgaac actttaccac ttcactggta
     7681 gaggtcgaaa cattcacctc cgcactgtta gaagaatttt ccaaggaatc aggagcctta
     7741 gcacaaccaa caagtcttta tatattgtgc agccgatatt ttcttcattc agtacaccct
     7801 tgaaatatgc agtgttagat aaagcagttc gtcctgattt gctgaggcac ccatctcttc
     7861 ttgaacgtac tccttgattt tcaaggccgc ccgatactct ttcggaaaac gctgcttcac
     7921 tgaagccagc aaaaaatcat ccttctccgg caactcttcc ttcttaatca ttcgggcaat
     7981 aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc gaatctttat caaccttttt
     8041 ttcgggaaac agatatccta gcatagccaa gatatccttt agtgctctta ccgagagcat
     8101 ggtttcttcc atatttgctt gttcactttg ggcatttaca atgtgaaacg caatgtttcc
     8161 agcctcctct tctggcaact gaacattcat ttttttgttg atcaattgga tagcatagca
     8221 gccgatctca aattcttttg ggtaatactt ttgaatttca atcagcaggc gattttgaaa
     8281 aacaatgcct ttttgactgc gttcaatggc atgcttcaag tgatcaacca aggtaaaaaa
     8341 tagactttta ttcagcttaa agtcaaactt ggcgttggca taatcgataa ttcgcttgac
     8401 gcaaataatt agatcatccg gagcagcctc aaacagttta atgtaatcct tttttagagc
     8461 atcagaaagt gaatctaatt taaatacttt ttggattttg tcagccggaa ccaaatcgcc
     8521 tttctttagt tgaaatccaa tccctttccc catcaaaatt acttctttgc cttgcttatc
     8581 taagcaaaga attaaactgt tgtttaacgt tttaattact ctcattagat tcaccgttct
     8641 ctctaagcta agccatttct tgcgattact ttttgatacc aaaagaagct ttcttttttt
     8701 attcgtttta attctttaag attctcatcg gttcggtcaa catatataaa accataacgc
     8761 ttattgaagc cttgatgggt actaacaaca tcaatcgctg accaaggtaa ataaccaaaa
     8821 acatctacac catctgttaa agctgcttga atagccagaa tatgtttggc tagataatca
     8881 attcgatcct ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc cccatagcca
     8941 ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca atttcctcaa tgttaatcgt
     9001 agtccagtag ggtcaactac ccagccatat ggcgttttat ctacataagg attttcagct
     9061 gcccggtaga caccttcttc tcccaacatg atctgttgat cccccgctct agcggaaaca
     9121 tctgatccgt catttttgct ggcagcgatg gtggcagttg aataataatt aatcgcaata
     9181 aaatcgggtt taccggagga taaaattgcc tgatcctctg cctcaatttt aggatataat
     9241 ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac tgctacatct
     9301 aagaaagacc agcctctcaa tgtttcccaa ttatgggctg caattgcatc ttctggactg
     9361 gaggtagctt gatacatagc cgttaaattg attgctggac cgatttttcc tttaagattc
     9421 aactgatgat acaagtgaat tactctagct tgagctaata aaacatggtg attaatttga
     9481 aagagttctt tttttgaagg cagctctcca cttggtggca aaccaatcgc tccaggatga
     9541 aggatcatcg tgttttgctc attaattgtc agccaatagt tcaccttgtc accatactca
     9601 gtaagcaata cctgtacata gtccacaaaa taatcgatag ttgctcgatt taaccaaccg
     9661 ccctgctcct ctagaaaata aggtaaatcg aaatggtaca tggtgacaat cggttcgatt
     9721 ccctgattca ggcattcatc aattacttga tgataaaaag aaattccctt ttgattggtt
     9781 ttattatttt caatatccat aattcgcgtc caagcgatgg aaaagcgaaa agcttttaag
     9841 cctaattctt taaacagctg tatatcttct ttatagtgat ggtaaaaatc cgaggcaact
     9901 tcaaaattag ctaccccttc tgtatgctga tacttgtcaa taatagacgg ttgtttccca
     9961 tcctcctgat aggcaccctc tgcctggtag gcactaatgg aggcacccca taaaaacttc
    10021 tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc tctccttaac taatttgcat
    10081 caatggttcg tgttccttta agcttccgct tgcttcacta attcctgcgt aattttcact
    10141 gttggtcact accacaatca ctgtatcatc gtagccagcg tttctaatgg cagcgaaatc
    10201 ggcgttgatt aataattgtc ctgctgacac ctgatctcct gcttttacaa atacttcaaa
    10261 gggttcccct tttaaattca cggtgtcgat ccccacatgg atcaataatt caataccttc
    10321 tttagaacgt agaccaatcg catgtttaga ttctaaaata gtaatgactt ctccctcaac
    10381 aggtgaataa atgctgtttt cttctggatg aatggctacg ccctttccca atagtccctt
    10441 agaaaagaca gcatcgttca cttcagaaat aggacacacg gttcctttta ccggattttc
    10501 aacaatgact gcttcggcta ttgccgtttg ctctgattca tcagaagtat cgtcaaatcc
    10561 aattagataa gcaatgataa aggttgcaac aaaggcgatt acacacgtaa taattgcgtg
    10621 aacaatattc attggatttt ctccaataaa ggctggtaag gctgctaagc caggggaaac
    10681 aaaggcatat ctgactaaac cgctcaagcc agcatacaaa ccagcaattg caccaccaat
    10741 catggaagca actaatggtt ttttcagctt caaggttact ccatagagtg aaggctcagt
    10801 aattcccatt aaagcagtga tcccagtgga cacagctagc tgcttcaact ctttgttttt
    10861 agttttcgcg gctacagcaa aggtagcacc tgattgagca atattagaag ccagcattcc
    10921 tggcccattg atcatttcag aacccgtact gctcaattgc atagtagcga ttggcgtcat
    10981 tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca aaaatggaat
    11041 taaccaagct gctttgccgt taatccattc agcccccatt gctataagat cgttcagcaa
    11101 cgttccaaac ggtccaatga caaccaacgc taaaggcccc ataattaaaa ataccagcat
    11161 cggcttagta aagaatttaa taaacgaagg cgaaattcga tccgcaaagg gctctacata
    11221 ggacatgatc caaacagtaa agataatcgg taaaactgat cccgcataat ctgcagctaa
    11281 taccggaatt cctagaaaag aaacggcatc gccagtagaa aaaagctgtg aaatatttgg
    11341 atgaagcaaa atgccagcaa cagtaatcgc caaaattggg ttgacatcaa actttatggc
    11401 tgccccatag gctaatagta ctggcataaa ataaaaggct gcatcagcaa tagtattcaa
    11461 aatgatgtaa gtttgactac tgtctgatag tacaccagtc aaagtcaaga cggccaaaat
    11521 ggcttttacc atcccggcac ctgttatagc tggaatcatc ggtgtaaagg tggtagaaat
    11581 gacactaatc aatcttgaaa taatactttc ttgattgtct gaagaggggc cctttttaat
    11641 tgaagttccc acttcctttt gaatttcctt gaaggctgtt tggacttcat tgccaatcac
    11701 cacttggtat tgtccaccgc tatccaccac actaattaca ccaagtgttt tttctaaggc
    11761 tttcgtatcg gctttccttt tatctttgag ttcaaaacgc agtctggtgg cacaatgggt
    11821 taagctgacc acattttctt ttcccccaac ctgttgcaat acatttcttc ctacttctct
    11881 gtaatccatt attctctctc cttaaagcag taaaaaagac caagtcaaac cacgccccaa
    11941 tcaacattga agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac atgccaataa
    12001 cgttattttg ctgtaatcca ataatagcac attatgatag cgcaatcaac agggaattta
    12061 attttttatc ttttcgtttg accgtatagg gtaatataac agttagatca catcaactag
    12121 ggtatatctg cgtatacttg ctgctaaaat aagtatatct aatattaact ataccagtaa
    12181 aaccattcct acataaccag cgggaaattc ccaaaatcgt gaaagcatca aaaatgcata
    12241 taacggatca cacaacttca acaattctgc tcttgggaaa aattgatgaa tttggtgagt
    12301 aaaggatgga ataaacagca ttccaacaat ataggcaaca atagtgatac ttgttttttt
    12361 ggtaaaaatc agtaagcaat agccaatttc aatcattccg agatataaag gtatcgatag
    12421 aatgatttgg gctagtattc ttaaaaatta actagcaaat gtttaaactg atttatttct
    12481 aatctacatg tagtaagtat ttcaattcca ccaattatca ataaaaatac caaacatatc
    12541 cataataatt ttttcttttt catttctttt atattgttat aacagtgaat aacataaaaa
    12601 ttcccgacaa tcattgtaat atttttttgt cacctgtatt agtatttaat ttataggaaa
    12661 ctataaaaac tccctagtga atttacacct ttgggagttc ctgtttggac ggtatgcata
    12721 atacatttaa atcaatttaa cattaacacc tcagcatatg gtatctccac atatatacgg
    12781 tctttctttg gatagtactc aatatttatg ttgatagcac ttccattgtc taatatagct
    12841 ttagcccggt atgtgttatc tccattttca tgagtaatcg tcacatcatc acatgatcga
    12901 tttaagttgt actgttcttg taaaatctgt gttaccaatg gttttgatga cttctctaat
    12961 ccagatgtac ctgtcatttt tgaaaaaata atacctataa tagccaagac aacaatacct
    13021 ataatgacaa acttcttgtt catatttact ttattcaact ttttatctcc tatctccaaa
    13081 ataatttgct cttatccctc ttatcagtac attgacatct ctaaaagtac accaaagatt
    13141 gctccagcta cccctgctat catcaaaatc gtacccattt tctcatcttt ttctcgatat
    13201 aagtagccca taataacagc aacagcacca aatacaacag gaataaatgc taacgatact
    13261 accatagaaa tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt
    13321 tgttccttca tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt
    13381 gtctcatctg tttcttgtag tgccccacaa aactggcaat acttactttc atctgttatt
    13441 tctttgccac attttctaca atacatgtct tctcctccta aaatcattca attgcttatt
    13501 agttaactgt ttactttatt aaatacatca gaaaacagct cattcattgg tacacttaat
    13561 atctcagaaa gcatcttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa
    13621 cttacacatt gctgtgtaac attcattttt tttgctaact ccgtttgaga aagtcctttt
    13681 tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt
    13741 atatacaaca atatgatgta tttcaactca taaaatataa aaaactttaa ataccattac
    13801 aaatacaaca acttgttgta ctatactgag atgggggtgt attaatgaaa cagaaacaat
    13861 atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg
    13921 aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta acagagccta
    13981 acctcgatag cttaacagct ctttctagag ccctaggaac ccctgtaagc cattttctag
    14041 acaatgttgt cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg
    14101 aagatgcagt tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca
    14161 atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg
    14221 agatgaaata agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt
    14281 ctcttcctta ctctccgtcc atcatctctt gttacacttg taaattgata cagaggaact
    14341 atcccagctc ctgataatat caattctttt attgtttcat ctatccctgc ccctttcgct
    14401 aaatatatta aaaaactact acttgctttc acattgtata gtttcttaat atccttagaa
    14461 gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttttat taaagcattt
    14521 ttcacgctgt tatccccaac attacgtagg cgttcacaaa cagtgttaat aagttctttc
    14581 tctgtcatta gatatgtccc ttgccacgat aagccctgta aaacttcatg tgcttcatct
    14641 aaaatcaatt gtttttcctt gctgagttct gatcgggaat tttgaggaaa tactctttta
    14701 gcctcttttt cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa
    14761 tgaatttttt ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca
    14821 ttaaaaacgt ctaatcgata gacattgctt ctcttatgat taaagtttcc taacgctttc
    14881 tcttccttta acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat
    14941 ttttttatca atcctataaa agtcataagt gatagtaact tattacattt atctatgtca
    15001 taagttttac catagtaaga actcattttt ttagctaatt gttcccccga caaaatacaa
    15061 gtcacacttt ccttactatc atcaaaaaaa ttaacatcag aaagcaagac atcatagaca
    15121 cgtcgcattt cctctagata attccttatt gtcctgaaaa gtttgtcttt cattgattca
    15181 agagtgtcaa aaagtacttt tgattcctct ttcaattttc gaaaatgtgt aggatacaag
    15241 tctaggttct ctaatagcca attaaatgtt tctacccgac tgtctttctt taaaattagc
    15301 atcacaactc caataaaatt ttcactgata tcacaattac tggaatgaca ataaaaaatg
    15361 gaatgcccag ttttactggt aaaaatacta gcagagggat tatgatcgtt gtgaaacaga
    15421 catcttaagt ttttacttga cttaagtaat ttattcatat caatggttaa caatctttca
    15481 taaatctcat taaaagacat gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc
    15541 atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt gactagttca
    15601 tactctctcc ctgaatcact ttgacaattt ttagaacatg tcatggtaaa atcaatcgga
    15661 agctcagtaa tatctaacat gttgtcttca ttaattatta tcactgcatc tgacttatat
    15721 cctccaaaaa acattctatt cgaagcattt acattgtcat cacacccggg gatgttcttt
    15781 ttcaagtatt cgtaaacagc aaccatttta ccatagtcag taattgcttc ttttaataga
    15841 aagacaatct tgtatttttc tagattttca gagtaccgaa tacttttttg tatcgcaaac
    15901 gcagaattat gaactaaatt gatagataaa accttttcta tgcttaagta tctactatca
    15961 tctttactca aatctactac cttccccttc tcatctttaa cagtgttatc gacatccaga
    16021 ataattactc tggtttccac aaaactctcc ttgtcattgc ctttattctt tgtcttgctt
    16081 ggaataaatg attgtgatga atttagtatc tctacaaact catctcttgt aacttctcta
    16141 tagttatcta aacacctatc tcttacatca gttttttctt tttttgtaac tggcttctct
    16201 aataattttt cttgatctac ataaattgga atattaatca ttgatgtttc catttgtgtt
    16261 tcctccttat aaaattctgt taaagactaa aacatacaga taataaaaac aaactcttta
    16321 ttactatatg ttttagtctt atttgtttac tcaaccttta taccgttcca tgaaactatc
    16381 aatatcagat tttttaaatc taatgaccgt atcaattttt ataacaggta acccgtattg
    16441 agaaacatat tttctaatcg ttcctactga tacacctaga tattcacttg cctgctgata
    16501 gtccatatac tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt
    16561 aattatctct ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag
    16621 agacatattt ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt
    16681 attttttgct caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt
    16741 accagttcat cgacatacac ataatacctc gataaaaaag gggtgtcaac agctttgtaa
    16801 aatttattta caacacccct ttttttgagg tatactaatt ttaggaggtg tggtcaatat
    16861 gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa
    16921 agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg
    16981 tcaattaatt gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc
    17041 agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg
    17101 caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg tactagatgg
    17161 tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca
    17221 ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt
    17281 tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt
    17341 aaaaatagaa gaattattca tcaatgttat tgtagaagaa tttgacaata acattctttt
    17401 cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc tacctattca
    17461 attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa
    17521 gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg
    17581 tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt aatggcaaaa
    17641 atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc
    17701 accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa
    17761 gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa
    17821 gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt
    17881 gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca
    17941 tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa
    18001 tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc
    18061 tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga
    18121 aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg
    18181 gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac
    18241 acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat
    18301 aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa
    18361 tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac tctttctatc
    18421 ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc
    18481 tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt
    18541 aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg
    18601 tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa
    18661 caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat
    18721 aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc
    18781 tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat
    18841 atcaagggtt ctagctccta taacaggttt acccaacact gccttccatc tcataactga
    18901 tcaatcggtt gagttcgact gcatattcca ttggaagttc tttggtgaat ggttcaacaa
    18961 agcccatgac gatcatttca gtggcttcag attcggtcag gcctcggctc atcaggtagt
    19021 agagttgttc ttctgagatt tttgagactt tggcttcgtg ctctaaagag acttggctgt
    19081 tgtggatctc attgaatggg atcgtatcgg atttcgacca ttcgtccatg atgatcgtgt
    19141 cacattcgat atgagaaatc gaactcgcac tgtttttggc aaaggttact tgtcctcgat
    19201 agtttacttc gccgccgtct tttgaaattg atttagagac gatagaacta gaagtgtttg
    19261 gtgcgttgtg gatcattttt gcacccgtat cttggatttg gtttgctcct gcaaaggcga
    19321 tggagagcat ggtgcctcgt gcaccttttc catctaagta aacacttggg tatttcatcg
    19381 ttgtttttgc acctaggttt ccgtcaatcc attcaacggt tgcgccttcg taagcttttg
    19441 ctcgtttggt tactaagttg tacacattgt ctgaccagtt ttggatcgtg gtgtaacggc
    19501 aatacgcatc ttttcttgta aagatttcta cgatggcggc atgcaaacta ttacttgaat
    19561 aagtaggtgc cgtacagcct tcgacatagt ggatgcttgc tccttcgtca acgatgatca
    19621 gcgtacgttc gaattgcccc gtgttttccg cattgatccg gaagtacgtt tgtaatggga
    19681 catcgacttt gacgcctttt ggcacgtaaa taaaggtccc gcctgaccat acggctgaat
    19741 tcagcgccgc taatttgttg tcggttggcg gtacaagttt tgaaaagtac tctttgaata
    19801 attcagggta ttctttcaaa gcagagtcag tgtcagtaaa gacgatccct aatttttcga
    19861 attcatcttt catattatgg tacacgactt ctgattcata ttgtgcggaa gcaccggcta
    19921 gataagctcg ttctgcttct gggataccga tcttctcaaa tgtatctttg attttttctg
    19981 gtacgtcttc ccagtcacga gcgggtttgt cacttggttt ttggtaatat ttgatggcat
    20041 cgaaatcgat gtctgaaagg tcgggtcccc atgtttgcat tggcatttta ttgaatgttt
    20101 ctagagattt cagacggaat tcaagcatcc attctggttc gcctttgatt cttgacatct
    20161 cacggatcac ttcttctgtc aagccttctc ctgtactgaa aaccggttcg acatcatcgt
    20221 gaaacccgaa tttatattct tctaattctg gtacgcccat gttctcactc ctcttccttg
    20281 tggtaatgat ggaaagctgc ggtaccatcg tttttcattg cttttcctag tgctttccag
    20341 gcaagtgtcg cacatttgat ccgagcggga aatttagcta caccaccaag catagcagca
    20401 tcgcctaatt ggtcgggatc ttggacttct tttccttgga caagctgtag aaagtctaca
    20461 gttaaatgtt ctgcttcttc tattgatttt ccaatgacgg catctgtcat catcgaggca
    20521 cttgccgtac tgatggagca gccgctgccg tcaaaggcga tatttttgat gattccgtct
    20581 tttacatcga cttgcaattc gatcacatct ccacatgtgg ggttgttcaa ttcgatcttg
    20641 cttgtcgagt cggtcaagct tccacgatga tgcggatggg aagaatgatc caagatcact
    20701 tgacggtata aattgtctaa tttagatagt cccatgttgg aaaaactcct ttgttgccat
    20761 gattgcttca actaaacggt cggcgtcgtg cgtcgtgttg tagaaataaa agctggcgcg
    20821 agcagtcgct gatacttcaa ggtacttcaa tagcggttgt gcacaatgat gacctgctct
    20881 aacggctacc ccttccatgt ctaaagctgt cgcgacatca tggggatgta acccatccaa
    20941 attgaacgca acgactcctg tccgaaaatt cgggtcttgc ggaccataaa cagtcaatcc
    21001 atcgattgct tgtaattttg gcaacacata agcggctagt tcttcttcat atcgatggat
    21061 attgtccatg ccgagttctt gcaaataatc gattgcatgg ccgaatgcga tagcgccagc
    21121 gatatttggt gtccctgctt cgaatttcca aggtagttct ttccatgtac tgtcgtataa
    21181 ttcgacaaaa tcgatcattt cgccaccaaa ttcaacgggt tccatttctt ctaaccattt
    21241 tcgtttacca tataagacac caatacctgt tggtccgcac attttgtgtc cactgaaagc
    21301 aaagaaatca cagtctagtg cttgaacatc aatggtcatg tgcggggcag actgtgcacc
    21361 atctactacg atgaccccgc cgttttcatg gacaagttca gctaattctt gaacaggatt
    21421 gatgacacct aagacattgg atacatgagc aatggaaacg attttggttt tgttcgtgat
    21481 ctttttcgct gcatcctcca tatccaggaa gccttcttct gttagatcga tatatttcag
    21541 gacggcacct gttcgttttg ccaactgctg ccatgggata acattagaat gatgttccat
    21601 gtaagagagc actatctcgt ctcctgcttt gatatagcgg tcgccgaagc ttctggctac
    21661 ccaattcaag cttgtcgtcg taccacgagt aaatagaact tctgctgttt cttctgcatg
    21721 gatgaatgcc cgtacttttt ctcttgcttg ctcatactca tgggttgcgc gttctgctaa
    21781 cgtatggaca ccgcgatgaa cattggcgtt atcgtgttcg taatagtgcg ctaatgactg
    21841 gagcacttgt tttggctttt gggtcgtcgc tgcattatcc agataaacga aaggttcatc
    21901 attgacgtct tgaaataaaa taggaaaatc attgcgtagc tttttagcat cgatcatcca
    21961 ttcaacttcc cttcgattac ttcgactaat tctttccgaa cttgttctac agggattgct
    22021 gttaagacag aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc tttatgcaat
    22081 cctcgactca tcagatagta catttcttct ggatcaactc gtcctacact ggcagcatgt
    22141 ccagcagtta cttcgttttc atcgatcaaa agaataggat tcgcgtcacc acgtgctttg
    22201 tcagaaagca tcaagacacg gctttcttgt tgggcatctg caccttttgc ccctttcagg
    22261 atgtggccaa taccgttaaa ggtcaatgtt cctttttctc ggatcactcc gtgttgcaaa
    22321 atatgcccga ttgtatgagg gactttattg gttactcgtg tatcgattcc ttggatttgt
    22381 ctgcccgagc tgattgccac gattttgact tctgcgtgtg caccttctcc tgctaaatct
    22441 gagtcaaagt cagcgatcac atgaccgtca ttcatgacac caattgccca gtcgacagaa
    22501 gcatctcgta aaatgtgccc acggcgattc atataagaag taatattctc tcctaattga
    22561 tctacagctg aatatttgat ttttgaacca gctttagcga tgacttccac gatgatgtta
    22621 cctgatgatt ttgctacttg ttctttggtt gtctggaaac gttccagata agaaaattca
    22681 ctatgttcat ctgccacgat caacacatgc ttgaagaagt gctcatcaga tgcgccatct
    22741 tgaatgaata aggactcgag tggttcatcg atcacgatat ttttaggcac atacaggaaa
    22801 ataccgctgt tcataaaagc tacatggagt gcagtcaatt ggtcttcgtc catgtttact
    22861 gcttttttca tataatattc ttggactagt tctgggtatt cgatcattgc agtgaataag
    22921 tcagtaaaaa tgactccttt ttctgctaat tccacaggca gttgttcaaa aatcgtccat
    22981 gaaccttgtt ggacaagcac aggattgtct ttcattgcat caaatgccgg aatcgtgcct
    23041 gtttcactga tagtttgtgt ttctttcaca tcgaataacg accagcgatg gaatttcacc
    23101 cgatcgatat gcggcaatgg cagttcatct gcttttgtta gtgcgtcttg acggaaggtt
    23161 ctcatccaat caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtt aagataatcg
    23221 agccagtttt tttctttcat tcttgctccc ccttacgctt cttctttata gtcgattcct
    23281 aattctttac tgattccagc gtagccttct gcttccaggc gtttagctaa gtcagcattt
    23341 cctgtcatga ctactcgacc ttccatcatg atgtggacga cgtcaggtgt gatatagttc
    23401 aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg catttcattt
    23461 actcctttag caacgacttt caaagcatcg atatccagac ccgagtcgat ttcgtctaaa
    23521 atcgcaaatg ttggttctaa catcaacaat tgtaggatct cattccgttt cttttccccg
    23581 ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt caataaagcc
    23641 attttttcat ctaacttttt caaaaatgcc ataacagaga tcttgtcgtt ttcatcacgt
    23701 ttcgcattga ttgctgcacg catgaattcc gcattcgtga tacctggtat ctcacttgga
    23761 tattgcatag ctaaaaacaa gccaagtcgt gcacgttcat ctacttccaa gtctaaaaca
    23821 ttttttccgt taaacaagat ttcaccttct gttacttcat agtttggatt gcccatgata
    23881 gcggcagata aggttgattt tcctgtgccg ttaggtccca tgatggcgtg aatttctccg
    23941 gttttcattg ttaggtttac gcctttaagg atttcttttt cttcgatcga cacatgtaga
    24001 ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt tctcactcat
    24061 tttagactaa ttgagaatga agcgcaatga tcttccttac taaaatcaaa tatcttttat
    24121 tgattattaa acaattttga tttctaaatt agaataggta taaaaaagat gaggaataag
    24181 ccgttacaga cttatttctc atcttttttg tttcattcct tattttgcat cagcctttac
    24241 tggtttaaca gagccgcccc atttgtctgt gatccattct tggacttctt tacggtgaag
    24301 cacttcaatc agttttttga ttttgtcact gtcttcatca cctttgcgag tcgcaatgat
    24361 attcacgtaa ggagagttgt ctttttcaag taatacggca tcttctaatg gattcaatcc
    24421 tgcgccataa gcgaagtttg cattgattgc gactaggtca ccttcatcat tttggtacgt
    24481 agaagctaaa agtgatgggt cgatcgtatg gttgaacttc aagttcttag ggttttctgc
    24541 aatatcatcg aatgttgctg tttccagatc tactccatct ttgactttga ctagcccagc
    24601 atcttgtaaa atcgtgatga tgcgtcccca gtctgattca gaattcgaag tgatgatcgt
    24661 tgcaccatct tttagttcag aaacatcttt gactttttta gagtacaagc ccattggttc
    24721 gatgtggaca gcaccggcat tgacaaagtc ataccctttt tctttgattt ctttgttcaa
    24781 ataaggaata tgctggaagt aattggcatc gatatcgcct tcttccaaag attggttcgg
    24841 taaaacgtag tcatcgaatt tgacgatttc aagagcgatt ccttcatctt tcaataatgg
    24901 tttcacatgt tccaaaatct ctgcgtgcgg tgtcggcgaa gcgccgactt tcaatgtcgt
    24961 gtcgtctgct ttatttgctg aatctttgct gtcgctgttg ttaccgcatg ctgctaatcc
    25021 tacagtcagc aaaagcgctg ctgctaatcc aaaaatcttc tttttcatat cctatatccc
    25081 cctatatcct attatctttt gtctgttttc ttagttaaga aatcaccgat tccttgaacg
    25141 ataaatacga ttaataaaat aattaaagtc gcaacgaatg tcactgtcag attaccacgc
    25201 tggtagcctt cctgccaagc aagacctcca agtccacctg cgccgatcgc gccggccatc
    25261 gcagtaaatc cgatcatcga gatcgttgta actgttaatc cagaaatcaa tgcgggaata
    25321 ctttcaggta tcaagacctt ccagatgatt tcccagtaac ttgctcccat tgcatctgct
    25381 gcttccagta caccagggct gacttcacga aaagcaatct ctactagacg ggcataaaat
    25441 ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg cacctagcat agtgcctacg
    25501 attgctttag taaatggaat gatcaaaacc atcaggatca taaatggcgt tgaccggaaa
    25561 atattactga tgatcgatac gattccataa atgattcgag catacggttt gtttttgcgt
    25621 ccaatagagt aaaggatcaa tcccaaaact aatccgatga tcgtcactaa gaccattgag
    25681 attaatgtca tatatagtgt atcaaggata gcctgattca tcgtttccgg gttgatttgt
    25741 ttaaaatcta gatatgtttc tgaaaagctt ttatccacga tggatcacct caattcctac
    25801 tccttgttct ttgatgaaca cttctgcttg atctaattct ttggtttcac cagtcatcaa
    25861 gacagtcaat gaaccaaagg aaccttcttt cacttgtttg atctttccat acacaacgtt
    25921 gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact ggctcgttgg catttctttc
    25981 gctaaaggtc aaagttgcca aacggccgct tggattttct ttgatgagtt cagctaatag
    26041 ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca aagcgtttgg ttacttcttt
    26101 tttcggttgt ctgaagacag tcaatacgtc tccttcttct acgactttcc ccaactccat
    26161 cacagcgact ttgttgcaga ttttgcggat cacattcatt tcatgtgtga tcaagacgat
    26221 ggtcaaattc aatttcttat tgatcgctaa caataagtct aatacttctt ctgttgtttg
    26281 cgggtccaat gcgctagtag cctcatcaca aagcaggatc tcaggatcat ttgccaatgc
    26341 tcgtgcgata ccgacgcgtt gtttctgccc gccggaaagt tgtgaaggat atgcttgtcc
    26401 gcgtccttct aacccgacta aacgcaacag ttcttctgcc cgttctttac gtttgctctt
    26461 tggtacgcca gctaattcca gcggcagctg gatattttca agtacagttc ttgaccataa
    26521 caggttgaaa tgctggaaga tcataccgat tttctttcgg aatgtacgta attctttgtt
    26581 ttttaatttt gtaatatctt gatcgtttac gatcacttcg ccgctcgtgg gaagctcaag
    26641 tccattcagg agacggacta atgtactttt tcctgcgcca gaatagccga cgattccata
    26701 aatatcccct ttttcaacgt ttagtgaaac gtcattgacg gcttggatgt ttccgttttt
    26761 cccattgaat gtctttttga cattcgttag ttggattaat gacatgccat tcacatcttt
    26821 ctcttatgat ttttcaaaca aaaaagcctt cgccctcttg ccttaagcaa aaggacgaaa
    26881 gcttgaactt ccgtgttacc accttgattt accgttactt cgcaataaca gcctcttcca
    26941 gtacttggac ttagcttctt tccgtatact gtcgcgctgt aacgggcact cccgtagtaa
    27001 gtttgcttac gctcgcttac tctgctctaa gaccatcttc catctcattt tcgttaccca
    27061 ttttcagcaa acagggctct cttcaaacgt ccgtgacatg tactcttctt ttcaaagctt
    27121 tctcgtttca tataggattg cttatgatta tagcaactgg aagaatcagt gtcaactttt
    27181 ttcaagtgat tttctgattt tataattcgt caaattcttt tggatcaaca tctttgaaac
    27241 ttaagatcca cgcattcatt tcatctgaat cattcaagat attgatatct tcaccgattt
    27301 tatcattaac agaagaaact gtacccgtta gcgggctggc aaattcattg accgcttttt
    27361 ccgcttcaac ttcgatcaga ggttcatctt tttttagcga ttgtcctgct tttggcagat
    27421 tggcaaacgt gatgtctcca agttcttctt gcgcttcatt tgtcaaacca acacagtatt
    27481 cttttccatt aaataagatc caaaggttgt cctttttctt caaatgcttg cctgccattt
    27541 tatatcagtt ctccttcata aattttttca ttgaatccaa ttgcaacaag ctggtcctct
    27601 cgtaagatca gtggccgctt gatcagcata ccatctgtcg ataagacttg gctcgctttt
    27661 gaagccgtaa actcgcctac ttgatctttc agtccaagtt cccgatattt catcccgctt
    27721 gtgttgaaca ggcgacggat tggtacttga ctcgtttcta gccaagtctc aatcgttgag
    27781 gccttcggta cttctttgat catatcaaca gtttgataac taacgccatg ctggtctaac
    27841 cacgctttgg cttttttgca tgttgaacat ttcgggtacc agtaaaaggt gtacaatttt
    27901 cttacctcct tgaagatctt gcaggtgctt cattttggta tctcatcgat aatatcaacc
    27961 cgataccgat catgttccct aaaatcgaag atcctccctg actaatgaaa ggcaatggga
    28021 tccctgttaa aggaagtaat ccgatatttg ctccaatatt ctcaaagaca tggaacagca
    28081 gcatcatgat caaaccgctt gcgatatagg cgtaaaattc attattcgta tcgaaacaca
    28141 cccgaatcat acgatagatt aaaataaaat acaagaaaat gacaaatgca ccaccgataa
    28201 agccgaagtt ttcaccgaca acggtaaaaa tcatatcgga ctcacgaaca ggcacataaa
    28261 tattgcttac gttgaatcct ttaccaaaca agccaccggt accaattgcc aaaagtcctc
    28321 gggccggctg ataactcatt cctgacgtat catggaaagg gtccagccat gaatcaatac
    28381 gtgcaaactg ataagatttg aagccaactt tgtaaagcaa gtctcttccg ccttcagtcg
    28441 taaccaagaa gatcgttcct gctccaacga ctactgccaa gattactaca ggtgcgatga
    28501 tgcgccaaga gattccagac atcaagaaaa tcccgccaaa gatcgcaaga aagacaagca
    28561 tcgttccaaa gtcatcttgg gccatgatca atgctaaaac cggtattgtc acgatcaaga
    28621 gcttcccaat cagccaaaag tccgatttca tcgtccgttc ttttacttgc gtattatgtt
    28681 tagttacgat cagtgccatc atcaagatgt acgcaatctt catcaactct gctggctgga
    28741 aagtaaacgc accgaatcga aaccagtttt tagaccctgt tgaagcagct aaattctgat
    28801 catagaattt gagcaagagt cccattacca ctaaccccaa accataaaag taaggagtca
    28861 gtttccagat ccatttggaa ttcaaatgca tgatgacaac gattgctgcc gctccaacta
    28921 aataccagac aacttgcatc cctacgcctt tcaagactgc tggattgttc ggatcatggc
    28981 taagtgctac atataatgag agcagaccaa tcaaacataa cagaaagact ggtaaaatca
    29041 cgccataatc aatacgatta tcgttcgaag cttttaattt tttttccatg tttcatccct
    29101 tttcttaaaa accatactgc tgattattat agcggtccag ttagataatg gaaagtcttt
    29161 ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag ttgcttgagc aaatgcagta
    29221 caaaagccta ctgcttatct ttagtgtatc atagaacttt ttttgataca tgcaattatc
    29281 taccaactga gattctcctc gctgtgagaa gtttctgtgc tttcattgta tcgaaggatc
    29341 tcttcggcaa tctctagcag ctgatcagaa accagattgg ttcgttggat gaaataaaaa
    29401 taacgatgga aattcaacgg agtcattggc acattttctg gaacagagcg cttagacaaa
    29461 atcgagcgac cgattccttc cttcagcaac gcaacaatta catcgttact tttcacgatc
    29521 atcttcttat cttggatatt catctcttca aaataacgct gcgtgtaatg aaaaacacca
    29581 gagtttgctt ctcttacaag ccacaatttg ctttttgggt ccccagcgat gactagctgg
    29641 tcttccatca aagagtatct ttgtacgccg gaagtctcta atggcttttc gataaaacca
    29701 aaatcggatt catgtttcgc tacttgttct aatgattcat gagaattcgc gatttcgatc
    29761 tcaaaatgca ctttaggaaa ccttttgatc agatgtttca tcaactgagg caaaggatag
    29821 acagcaaagg tgtgggaagc aacgattcga catacttctt cttcattttg ttcattgagc
    29881 aatttttgct gagtttcttc ccattcttct aacaaaacca gtgtccgttc atataataag
    29941 tcggcttgct tagttggaag catctctttt cttccgttac gttgaaaaag atcgatattc
    30001 aattcccgct ccaactgttt gatttgattc gagactgctg gttgagataa aaacagtact
    30061 tctgctgctc tggaaaaatt tcttgtttca tatactgctc gaaaagtcgt taataactta
    30121 aacatggcac cacttccata actttttatg ataattacta taaaaactat ttgatttaaa
    30181 tatacaaaaa accatctata atagaaatgt acaacaaaac agttgctgat ccaactagta
    30241 acttagatga agacaatcag ctgtctgctt gctcgtaatc atacattcct attcatttta
    30301 caatgtattc gttcatcttg gtactaaaat ctattaaata aaaaggagaa tattatatat
    30361 gaagaacttt ttgaaaatta ttccgatccc gatctgcggg ttgatccttg ggttagcttc
    30421 ccttggaaat ctgtttaaag actatcacca tgttgcttta ggcaatctaa ctggcggtat
    30481 ctctatgatt cttatgatat tcatttttgt taaattgttt atcttattcg aacacacgaa
    30541 acaaacgttg catgatccaa tcatcgcctc cgtttcacca acttttacga tgtcactcat
    30601 ggttatttgt acttattttg tttcatatga aagtattgca ccaattgtca aattcatttg
    30661 gctgtgcgct gttatcttcc aagtgatcct tgtcttttat ttcaattatc accatgttat
    30721 caaagctgat ctttcaattg aagcaattta tccaagctgg tttatcattt atgttggatt
    30781 tggggtcatc actgtaactg ccgggaactt cttcccgctt attggaaaaa ttttcttttg
    30841 gatttcattg atctgctatg cgttcttatt gccgatcatc atcaaacgtg tctttttcgt
    30901 aagaaatatg gctcatccga cacgtccgct gattacgatc gttgcagcac ctggttctct
    30961 ttgcctaaca ggttatctta aaaactttgc tgaacctaat ttttatcttg tcgtagcatt
    31021 gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct atgcttccac gtttgttcaa
    31081 gcttcgtttt tatccaagct atgcagcctt tacattcccg cttgttatct ctgcaacagc
    31141 tatatttact actgttcatt acttcaacag tttaggtatc tatagtagcg tattggattt
    31201 cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc ttctacgttc ttgctcatta
    31261 cgtacgtttc ttaattaaag aacatagcaa acatggaaaa tttgtttcta aaaaaatcgc
    31321 ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacggcc
    31381 aaacaaaaac agctgttcat gtttttgaat tggttgggct tattaccgca gatgcaagct
    31441 tttgaacacc gtttaccaag aggtcgtaaa agaagtggcc agcttcaaga aataagaagg
    31501 aatttacgaa aattgcttct caaatttttg tgaaatttcg gcttatttcc gaaaaagcta
    31561 cttctggaac accgtttacc aagaggtcgt gaaaaagccg cttagtttcg agtattaaga
    31621 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttggcttaat accgagaaac
    31681 tggcttttga acaccgttta ttcgaaacaa gaggtcgtga caagactttt gtcacgacct
    31741 cttgttcttt tgtcaaaata ctggtttagt gctactatgt tcgagtatgc ttttagtatt
    31801 tattgacgaa aggaaccgat caaatgaaat cattgtcaca tccaataaaa tctgtaaagg
    31861 attcagcggt ccgcgaaatc ttacccggtt tactgctttc ttttgccatc gctgccttca
    31921 gcaggcttct tgctatttat ctgccacagt taggtggagc gactattgct attttattgg
    31981 gtattttatt aggaaatact ttttttaaac agcctgttct agcaatcggc acaaaatttg
    32041 ctgaaagcaa actattagaa ttctctgttt ttttactagg tgcaactgtc acagctcaaa
    32101 cgattgctca aattggtata aaaggaattg tttttgtgtt actgcaaatg tcagtcacta
    32161 tcatcggtgc ttacttgatt ggtaaaaaac tgcaattcag cgatacttct tctttgatga
    32221 tggctggagg aaacgctgtt tgtggttctt ctgctattgc ttcgatcgcc ccttccatcc
    32281 atgcgaaaga ggaagaaaaa ggacagatca tcacactagt caatctactg ggtactgtga
    32341 tgatgttgac tctccctttc cttggattac agttgtttgg tggaaatctc attgaaaaaa
    32401 gtgcactttt gggtggcaca ctccaatctg ttggtcaagt agtcgctggg gcaagcttac
    32461 ttgacaattc tgttgtcgaa ttcgccatgc tatttaagat tctacgtatt atgatgctcg
    32521 ttgttgttgt ctatctgttt gaaaaattca tgcacaagaa acaaccagct ctgtttgaaa
    32581 cgccaactga tacgcagaaa aaacgaaaat taccacttcc ttggtatgta ttagggtttg
    32641 tgatcttttg tttggtcaac agcttgtttt ctttacctgt agttattgac caatcggcac
    32701 attttatcag cagctggttt gagatcactg ctttagcggc aattggtctg cgtttagact
    32761 ttgcaaaatt catgaaagaa ggtacacgct tcttatctta tggattagct gtcgggcttc
    32821 tccaaacagt gatggcttta ctatttattt ggctgatcaa agtttagaat gaacatgaaa
    32881 aaacagttgt gattctcgca tggaacaatg tccgaatcac aactgttttt aattttgcag
    32941 atatccttct aacaacaaca attggcgttt gatttcaacc ccatctattg cgtttcccat
    33001 atatcctgtc agttttccat tttgcccgat cactcgatga caaggaataa cgattggcaa
    33061 tggattggtt cgatttgcct gcccaatcgc tcgcacagct ttcggactat caacagcaac
    33121 agctacttct ttatacgttc ggatttctcc ataaggaatg gtcgacaaag ctttccagac
    33181 attttgctga aaaggcgtac ctctctttat agataaagga acatcaaatt cttttctttt
    33241 tcccgcaaaa tattcttcaa actgttcttg tgtgcgcgtt aaaatttgct gattcgctga
    33301 ttcgctttct gaaattggct gaaaagatac agcagtcaat ccggattcat ctgcatctaa
    33361 ccataaaaag ccgataggtg tgtgtatttt cattttgttt gctcctggtc agtagactgg
    33421 aacttcctcc tttttctcta tccaaaacta cttgccaact aataaaaatt gttcttaaaa
    33481 gtttgataaa ccgaggaaac atggtaaact aaatacgtta gtttaccaaa gaatagctgt
    33541 tcttttccat aaaatcaaat ttaaaaggtg ttatttaagg tttattaaat tttgaatgta
    33601 aaccatatat aaacatacga ttgaaaggat agaaacgatg aaagtatcaa ttgttgtccc
    33661 atgttttaat gaagaaaaaa ctgttcctct tttttttgat gaggttgaaa aattcaaagg
    33721 tgcccatgag ttcgaatatt tgtttattga cgacggatca aaagacggta cgttagcagc
    33781 aatcaagaag ctagcctcag cttatcctta tgtccgcttt gtctcatttt ccagaaattt
    33841 tgggaaagaa gcggctctgt acgctggatt gcaagcttct acaggagatt tagtgaccgt
    33901 gatggatgtt gatctgcaag atccgcccga actgcttcct gagatgatca accggatcac
    33961 tacttctgat gtcgatagtg tcggcacccg gcgttcgact cgtgaaggcg agcctgcgat
    34021 acgcagtttc tttgcgaaaa aattctacca gttgatcaat aaaatcagcg atacagaaat
    34081 ggtcgatggc gctcgtgatt tccgcttgat gactcgtcaa atggtggatg ccgtcctaga
    34141 attaacagaa tacaaccgat tttcaaaagg attatttagc tgggtcggat ttaaaacaga
    34201 atatatttct tatgaaaacc gcgaacgtgt ggctggcgaa acatcttggt ccttctggaa
    34261 attattcaac tactcgattg atggtatcgt gaacttctcg gatgcaccgt taacgattgc
    34321 ttcatttatc ggcgctttgt cttgtgtcgg ttctggtatt gcaattttgt tcattatttt
    34381 gcgggcattg ttgtttggtg atcctacatc aggatggcct tccatggtct cgatcttcct
    34441 gttcattggt ggaatccaat tgttatgtct tggaatcatc gggaaatata tcgggaaaat
    34501 cttcttagaa acgaaacagc gtcctgttta tatcataaga gaaacagaaa aagtgaagcc
    34561 aaatgaattg aagcctcacc aataatatac gtcaaatgaa gtaaacattt ttttaaatga
    34621 aaaaggactg tgacaaaagt cttgtgtacc gacccccaaa agttagacca aaaaatctaa
    34681 cttttggagg tcggtatttt ttatggctaa gtatactttt gaacttaaat taaagatcgt
    34741 tcatgattat ttggatggca aaggcggaag tgattatctg gcaaagaa
//
LOCUS       DAPBAB010000024        33031 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPBAB010000024 DAPBAB010000000
VERSION     DAPBAB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.024"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(41..2491)
                     /gene="parC"
                     /locus_tag="RD444_001424"
     CDS             complement(41..2491)
                     /gene="parC"
                     /locus_tag="RD444_001424"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDT7548807.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     VTIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(2503..4548)
                     /gene="parE"
                     /locus_tag="RD444_001425"
     CDS             complement(2503..4548)
                     /gene="parE"
                     /locus_tag="RD444_001425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDT7548808.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLTVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEVPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4714..5148)
                     /locus_tag="RD444_001426"
     CDS             complement(4714..5148)
                     /locus_tag="RD444_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDT7548809.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEVADDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5275..5913
                     /gene="plsY"
                     /locus_tag="RD444_001427"
     CDS             5275..5913
                     /gene="plsY"
                     /locus_tag="RD444_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDT7548810.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(6045..6920)
                     /locus_tag="RD444_001428"
     CDS             complement(6045..6920)
                     /locus_tag="RD444_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDT7548811.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNREIFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADAVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(7002..7793)
                     /gene="codY"
                     /locus_tag="RD444_001429"
     CDS             complement(7002..7793)
                     /gene="codY"
                     /locus_tag="RD444_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDT7548812.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7811..9211)
                     /gene="hslU"
                     /locus_tag="RD444_001430"
     CDS             complement(7811..9211)
                     /gene="hslU"
                     /locus_tag="RD444_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDT7548813.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALIKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9225..9773)
                     /gene="hslV"
                     /locus_tag="RD444_001431"
     CDS             complement(9225..9773)
                     /gene="hslV"
                     /locus_tag="RD444_001431"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDT7548814.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9810..10715)
                     /gene="xerC"
                     /locus_tag="RD444_001432"
     CDS             complement(9810..10715)
                     /gene="xerC"
                     /locus_tag="RD444_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDT7548815.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10913..12289)
                     /gene="trmFO"
                     /locus_tag="RD444_001433"
     CDS             complement(10913..12289)
                     /gene="trmFO"
                     /locus_tag="RD444_001433"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDT7548816.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLDSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12258..14336)
                     /gene="topA"
                     /locus_tag="RD444_001434"
     CDS             complement(12258..14336)
                     /gene="topA"
                     /locus_tag="RD444_001434"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDT7548817.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVIARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDIVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14458..15315)
                     /gene="dprA"
                     /locus_tag="RD444_001435"
     CDS             complement(14458..15315)
                     /gene="dprA"
                     /locus_tag="RD444_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDT7548818.1"
                     /translation="MYQIEENLLKLAYAKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITTHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVVEAGYVIVSGCAKGIDTYGQQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHRFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15371..16138)
                     /locus_tag="RD444_001436"
     CDS             complement(15371..16138)
                     /locus_tag="RD444_001436"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDT7548819.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16131..16991)
                     /gene="ylqF"
                     /locus_tag="RD444_001437"
     CDS             complement(16131..16991)
                     /gene="ylqF"
                     /locus_tag="RD444_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDT7548820.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            17252..17650
                     /locus_tag="RD444_001438"
     CDS             17252..17650
                     /locus_tag="RD444_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7548821.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            17929..18429
                     /locus_tag="RD444_001439"
     CDS             17929..18429
                     /locus_tag="RD444_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDT7548822.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(18512..19063)
                     /gene="lepB"
                     /locus_tag="RD444_001440"
     CDS             complement(18512..19063)
                     /gene="lepB"
                     /locus_tag="RD444_001440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7548823.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(19215..19442)
                     /locus_tag="RD444_001441"
     CDS             complement(19215..19442)
                     /locus_tag="RD444_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDT7548824.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(19439..19957)
                     /gene="msrA"
                     /locus_tag="RD444_001442"
     CDS             complement(19439..19957)
                     /gene="msrA"
                     /locus_tag="RD444_001442"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7548825.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(19977..20690)
                     /locus_tag="RD444_001443"
     CDS             complement(19977..20690)
                     /locus_tag="RD444_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDT7548826.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(20693..21589)
                     /locus_tag="RD444_001444"
     CDS             complement(20693..21589)
                     /locus_tag="RD444_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7548827.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(21747..22589)
                     /locus_tag="RD444_001445"
     CDS             complement(21747..22589)
                     /locus_tag="RD444_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7548828.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            22721..23374
                     /locus_tag="RD444_001446"
     CDS             22721..23374
                     /locus_tag="RD444_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDT7548829.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(23438..23965)
                     /locus_tag="RD444_001447"
     CDS             complement(23438..23965)
                     /locus_tag="RD444_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7548830.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(23985..24932)
                     /locus_tag="RD444_001448"
     CDS             complement(23985..24932)
                     /locus_tag="RD444_001448"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDT7548831.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(24944..26830)
                     /locus_tag="RD444_001449"
     CDS             complement(24944..26830)
                     /locus_tag="RD444_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7548832.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(26995..29067)
                     /locus_tag="RD444_001450"
     CDS             complement(26995..29067)
                     /locus_tag="RD444_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDT7548833.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(29080..29355)
                     /locus_tag="RD444_001451"
     CDS             complement(29080..29355)
                     /locus_tag="RD444_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548834.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(29717..30172)
                     /locus_tag="RD444_001452"
     CDS             complement(29717..30172)
                     /locus_tag="RD444_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548835.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(30326..31534)
                     /locus_tag="RD444_001453"
     CDS             complement(30326..31534)
                     /locus_tag="RD444_001453"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDT7548836.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(31646..31978)
                     /locus_tag="RD444_001454"
     CDS             complement(31646..31978)
                     /locus_tag="RD444_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDT7548837.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            32126..33001
                     /locus_tag="RD444_001455"
     CDS             32126..33001
                     /locus_tag="RD444_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7548838.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
BASE COUNT         8826 a         7024 c         5713 g        11468 t
ORIGIN      
        1 ttaatacaga tggcgtctct tcattaaaat actacaacag ttacgactcg atgaaaatct
       61 caggagcatc gataacttcg atgacttctc cacctttttt ctcatccatc gcaaatgatc
      121 cattcgatgt tcgatcacct atagggaaat cagttgggtc aatcgttaac tgctgccctt
      181 tttgattcat caaggttatt tcttttgatc ttccttccga catatagata atacggtgcg
      241 gatttttttt cagctcacgc atgaccgtca ctccccgctt cgcacgtgaa gtttggctga
      301 tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt aacgattacg atcggcgtat
      361 caccttctgc taacacaagc agcccgttga taatcctatc tccatctttc aaattcatag
      421 acttcactcc agctgccttt gctccaacaa cgggaacatc tgttaaagga tagcgcaaac
      481 ccattccttg atgcgttact aagaaaacat ctaagcctgg ctgatcttct ttcagataca
      541 cagcaatcag ttcatcttct tccgccttta gcttcataca gctgagcgga cggctcttat
      601 aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt gatcaatcca ttcttgctga
      661 tgaatacaaa tgttttctct gcttgaattt tttgatatgg gaaaacagct aaaatcgatt
      721 cgtctacaga cagattggag attgtttgag aaatgtgctc accagcatct ttccatttca
      781 aatcgggtag ttcatggaca gggcggtaga tcacatttgc tttatttgtg atcagaagaa
      841 catggtccaa tgtactcaat tcacctgagt atagcaagta gtcgccttct cgcatcccga
      901 tttcttctgg tttggaagca ctataagaac gaaggcttgt tcgcttcaca tagccttctc
      961 gcgtcacact gacaattacg tcttcttgtg ccactaagac ttgcgtatca attttgattt
     1021 cttcgatttc gtcttcgatt gtcgttagac gagcggaaga ataatttttt ttcacttctc
     1081 gcaattcttt tttcataaca gagaaaagct ctttgtcatt tgaaaggatt ttattcaact
     1141 ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag ctctgtgata tccgtgttcg
     1201 tcaaacggta taattgcaat gtgacaattg cttctgcttg ttcttctgta aattgaaaga
     1261 cgtcgactaa attctttttg gcgtcttttt tgtctttact ttcacgaatc gttacgatta
     1321 cttcatccag aatcgataaa gctttcatca atccttctac gatatgctgt cttttttgag
     1381 ctttggctaa atcgaattga ctacgttttg ttatgacttg ctttcgatgc tcgatataac
     1441 ttcttaaaat gtctttcagt ccaacttgat gcggagtcat atggtcgata gcgaccatat
     1501 tgaaattgta attgatctgt agctcagtgt ttttgaacaa ataattcaaa attccttgag
     1561 cattggcgtc ttttttcaat tcgacgacaa tttgcagacc tgttctatca ctttcatctc
     1621 gtacttcagc tattccatcg attttcttat tcaaacggat ctcatccatt tttttcacca
     1681 aggtcgcttt atttacttca tatgggattt ctgtgatcac gatttgctgt ttattaccct
     1741 tcattgcctc tatctttgtt ttcgaacgaa gaataacttt tcctcgccct gtttcataag
     1801 cttttttgat ttcatctttt ccttgcaaga tccctcctgt tggaaagtca ggtccaggaa
     1861 tatattccat caatttttca aggctcgctt gtggatgatc gatcatatag attgtgccat
     1921 caatcacttc agctaagtta tgagtcggga tttctgttgc atatccagca gatatacctg
     1981 tcgatccatt gacgagaagg tttgggtatc gagctggaag gactgttggt tctttttctg
     2041 tatcatcaaa attccacacg agatcgactg tttctttttc gatatctgct agcatctctc
     2101 cacttaactt ggataatctg gcttcagtat aacgcatcgc agcaggtgga tcaccgtcca
     2161 tactcccgtt attcccgtgc atttcgatca gtacttcccg caacttccag tcttggctca
     2221 tgcggaccat cgcttcataa atactgatgt caccatgagg atgataattc cccatgatat
     2281 ttccgacaga ttttgctgat ttacggaatc ctttgtcata cgtattgcca tctttgttca
     2341 tggcaaataa tatccggcgt tgcactggct tcaatccatc acggatatca ggcagtgcac
     2401 gttcttggat aatatatttg gaataacgtc cgaagcggtc acccatcact tcttctaaag
     2461 ttaattcttg tacttcttgt cgattttcca tcaggtgacc tcctattcta cttcaaataa
     2521 ctgattgttt tcatttttat ctgctcgttc ttcatctaat aaatcattag aaactgaagg
     2581 agagatttca gtatcctctt ttttatctaa gatactgcca tcttcttcga gcgtaaattg
     2641 cacgtgattt tcgatccatt ttcttcgagg ttctactttg tcacccatca gtgtggttac
     2701 tcgacgttca gcttgcgcag catcatcgat acggacgcgg atcaacgtac gggaagtcgg
     2761 atccatcgtt gtttcccaca attgttccgc attcatctct ccaagtcctt tatatcgctg
     2821 cagcatatat cctttgccga cttttttaat cattgctgcc aattcgtcat ctgtccacgc
     2881 atattcaatg acttgcttct ttccttgtcc tttggatact ttatataaag gcggtaacgc
     2941 gatatatact ttcccagctt caatcagcgg tttcatataa cgataaaaga aagtaagcag
     3001 taatacttga atatgggcgc cgtctgtatc cgcatcggtc atgatgatta ctttgtcgta
     3061 attacagtct tcgatagaaa actcaggacc gactcctgca ccaatcgtat agatcatcgt
     3121 attgatttct tcatttttca agatatcttg cattttagct ttttcggtat tgatgacttt
     3181 tcctcgtaaa ggtagaattg cttggaactt tctgtctctt ccttgtttag cagatccgcc
     3241 agcagaatcc ccttcgacta aatacaattc atttttctta gggtttcggg attgtgcggg
     3301 tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc ttcttcccat ttcggctttc
     3361 ttctctggct ttacgtgctg cttctctcgc ttctctggct ttgattgctt tacggaccaa
     3421 catctggctc atttcgctat tttcctgcaa ataaaagccc atttgttcac tgatgacgtt
     3481 atctactacg gtacgagcaa ctggcgtacc aagtttttct tttgtttgcc cttcaaattg
     3541 cagaagattt tcaggtacac ggatagaaag tacagcagct aacccttctc tgaaatcact
     3601 accttccaga ttcttgtccc gttctttcag caaaccaact tttctcgcat attcattata
     3661 agatttcgtc attgccgctt tcattccagc ttcatgggtt cccccatctt ttgtccgaac
     3721 gttattgaca aaagataaga cattttctga atacccgtca ttgtattgat aagcgacctc
     3781 tacttcgata ccttcttttt ctcccgaaaa ataaacaact ggtgtcaatg tatctttttc
     3841 ttcattcaga taatcaacaa attctttgat tccttcttcg taatgaaaaa tttccttgac
     3901 tggtacttct ccacgaagat cagaaagaga gattttaacg ccttttagaa gaaaggcaga
     3961 ttctcttaac cgttcggcta aaatttcata tgaaaatttc gttgtactga atattgtatc
     4021 gtctggtaaa aaatggacag tcgtcccgtt tgcttttttt gtttttccta tctttttcaa
     4081 cgtaccgtca ggtttaccgc cgtttttaaa tttctgctga tactctacgc catctctaac
     4141 gatcgttaca gttagccatt tcgataaggc atttaccaca ctggctccta caccgtgcag
     4201 tccaccagaa gttttgtacc ctccttgacc aaactttccg ccagcatgca gcacagtaaa
     4261 aatcacttca actgttggta ttccggaagc atgcattcct acaggcatcc cacgtccgct
     4321 atctgcaaca gttatactat tgtcttcatg gatggtcaca tcaatctcac tgccataacc
     4381 tgataatgct tcgtcaactg cattatcgac gatttcatag actaaatgat gcaagcctcg
     4441 gctgtcagtg gatccaatgt acatccctgg tctttttcta actgcctcta atccttccaa
     4501 tacttggatg gacgagtcat tgtattcgtt gtttactttt tttgccaagg caaacgctcc
     4561 ttcaataata ctttgcggca aaaccgcacc acgattatca tactagaaaa ggtcgtgaaa
     4621 aaaaagaggg tagacgagaa atcaatttta attatctcct aaccgctttt tcgacattcc
     4681 cctctatcga tcatctcttc tttgtggtca tttttatttt tggttttctt tcaacttacc
     4741 aagttcaatc ttaatacaac gatccatcac gatatctgta cgtcctgctt ttcttagcat
     4801 ctcagctgct tctttgtttt caatccctaa ttgtgcccag aacactttag catcagtttt
     4861 caaaaaatca tctgctactt caggtaaaaa ttcactccgt ctaaatacat ctacgatatc
     4921 gattggatca tcgatttctg tcaaattcgc atagactttc tcgcctagaa tttcttcacc
     4981 agccaataat ggatttactg gtatgatttt atatccattg tcttgcaata cctgtgcaac
     5041 catgtaactt gttcgttctg gtttattgct caagcctacg acggcaatat tttttgcttc
     5101 ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt acagacattt ctgccacccc
     5161 ttgcttttct ttttattcca tctattatac ttgtatcttt cattttttca attgttcctg
     5221 ttacctattc atgctaaaat aaaggatagt tttcaacttg aaagagggta aaaaatgaaa
     5281 ttgatcttat tacttatttt agcatatctt ttaggttcta ttccttccgg tgtctggatt
     5341 ggcaaattgt ttttcaaaaa agatatccga caacacggga gcggtaatac tggaacgacc
     5401 aatacatttc gtgtattagg caaaacagcc gggattgttg tcctgcttat ggatatttta
     5461 aaaggtacat tggctacttg tttgccgatg atcttccacc tttctgggat taaccctctt
     5521 tggtttgggg tatgcgccat tcttggccat acatttccga tttttgctgg gttcaaagga
     5581 ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat ttaatcctgt attttttgtt
     5641 tattcttgta tcatctttat cgttagttta ttttgtacaa gtatggtgag tttgagcagt
     5701 atgatcagtg cagtattgat cactttatct acgatcgtcc ttccttatgt agctcctgtg
     5761 attttggcta agcccaattg gttactgacg atcattgcct ttttagtttc ttcctttatc
     5821 ttttatcggc ataaagacaa tatcaagcgt atcagaaacc atactgaaag tcgaatcccg
     5881 tttggtttga atagcagcaa gaaaaaatcc tgatactttt tcattccaaa gagataaaac
     5941 gggagtgtga caaatcgatg tcacactccc ttattactta caggatagct tttttacaca
     6001 ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa cctcttactt cactgtgatg
     6061 gaatattttg tcttgaagat ttcatgggca cctagttgat tgatccctac tttttccatc
     6121 aagtttccac tactgttcac cgcgtcagcg attccgcacc acggttcgat acaaacgaaa
     6181 ggcgcttctt ttggataagg agaccagatc cctacataag gcatatcttt atagcttaaa
     6241 gttacgctat gggaagattt ttcacttcga atggcatacg catttagacc ccttgtttca
     6301 aaaaccaagg catcttgctc aaaaatttca cgattcaaag ccagattcgt atttgtctga
     6361 cctaacgtct tttgatccat atcgatgaat gggcctttca atggtaggcg taatcttgat
     6421 ttcaatggag aaaatgccag atagaaatct tcaaatgaca atccttcttc aagaggtaca
     6481 ttgaaagcag gatgtccacc gatagaaaag aacatttctt tttcaccggt atttgccact
     6541 tgataatgaa ccgtgatgcc gtctcctcct aattcatagc tgatcactaa ttcaaaatca
     6601 aatggatact tttctaatgt ctccggcgta ctccttaaga tgaaactagc catttcttgt
     6661 ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc cgtgttgccc catttgatat
     6721 gttttccctt gataaacata ctgatcatct tttaaacgtc caacaaaagg gaataaaacc
     6781 ggtgcatgtc tgccccaata cgctggatca ccttgccaga tatactctaa accatgttct
     6841 tttgatttca gactgatcaa ttccgcaccg tgttcagcga tttcggcgat cagctggtca
     6901 ttttgaataa taacagtcat tgccattcct ccaacttcga cttacttttt aaaatgggaa
     6961 ggctggaaca aacatttcta tctgccccag tccccttact tctaataact gtgttttgct
     7021 aactcgtctt taaaacggga attcaatact tttaaataag ttcccttcat gcctaacgaa
     7081 cgagattcaa tgattccagc tgattccaat tttcgtaaag cattgacgat cacagaacga
     7141 gtgatgccga tactatcggc aatattagaa gcagtcagac gaccttcatc tccatccaat
     7201 gcttcaaaga tagcttgtac agccttcaat tcgctataag ataatgtgtt gattgccatt
     7261 tggacagccg ttgcactccg tacatctgct tcgatgcttc gagacttctg ataaaggatc
     7321 tgcataccga caaccgtagc tccatattct gctaagacta gatcgtcatc tgcaaacgat
     7381 tgttccatac gagataagat aatcgtaccc aaacgttcgc cagctccaaa gatcggaacg
     7441 acagtcgtca gaccaaatgg atatttctct cttgactcta ccggaaatgc agtaatatca
     7501 ctagtaattg ggatattagc ttcagttttc atcaactgtg ccactgtatc agtataagat
     7561 tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt cattgttcac atcgtgacgt
     7621 tcattataac ctagtaatac tccgtcactt cctatgatat aggtgttgct gtcaagaata
     7681 ccacctagtg tgacagccat tttatcgtaa ggcaaatctg cttgcatgtc aaaagtattc
     7741 ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta gcaagtctgt cattgtcgtc
     7801 ctccgatttt ttataaaata tagcgactta aatcttcatt tgccgcaatt gagccaagtt
     7861 tgtcatccac atatgcttct gtgatcgtta tttctcccat ttgcatatct ggtgcttcaa
     7921 ataatagatc ttctagtagt ttttccaaaa tggtatgcag tctgcgtgcg ccgatattat
     7981 ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg ttcaattgct tcttttgtaa
     8041 acgtaactga cacattttct gtaccaatca atgctacata ttgttttatt aaagcattat
     8101 tcggttcagt cagaattttc acaaaatctt cagctgtcaa atcatctaat tctacccgta
     8161 ttgggaaacg cccttggagt tctggaatca aatcactcgg tttagataaa tggaacgctc
     8221 ctgaagcaat gaataagaca tgatctgttt ggatgatccc gtattttgta tttacttgtg
     8281 aaccttcgac gattggcaat atgtctcttt ggacgccttc tctggatact tctccagatt
     8341 gctgtgattt agaagtgatc ttatcgattt catcgataaa aataattcca ctttcttccg
     8401 ctaatttgat tgcctcacta tggatatcag catccttgac taattttgct gattcttcct
     8461 tgatcaataa ctctaaagct tctttgactg ttaccgtccg ttccactttt ttcttcggtg
     8521 tcaaagcacc tagagtttca tttaggtcga tccccatttg ctccaatcca ttgttcatca
     8581 ttggtgcagg tttcttttgg tcttcaactt caatggttac ttcacgacta tccagtaaac
     8641 ccttttgtag ttgttccaag actgtacggc gattgattcg aatatcttct gtcacttctt
     8701 cttgcggttc agcggtattt tgctgactag cattaaaaag attcatcatt tgttcaaatt
     8761 gattagtgga ttgtttctgt tcttttttga taccaggtac caagattttt accaaacgtt
     8821 tttctgcatt ttttttagca cttgtgtata cacggctgta ctgctctttt tctacgattt
     8881 ggatcgcatt ttctactaaa tcccgaacca ttgattctac atctcttccg acataaccga
     8941 cttcagtaaa ttttgttgct tcgactttga caaatggtgc attgacgatt tttgccaaac
     9001 gtcgtgcgat ttctgtttta ccgacccctg taggtccgat catcagcata ttttttggtg
     9061 taatatcttg ttgcattttt tcatctaact gcaaacggcg atagcggtta cgcaaagcaa
     9121 cggcaacaga tttttttgct gcgtgctgtc ctacgatata ttcatctaat tcacttacga
     9181 tttgtcttgg tgtttgtttt aattcataca tatctactca cctcttacag ttcttctacg
     9241 ataatattat gattagtaaa gatacagata tctgcggcaa tatttaacgc attttctgca
     9301 atttctttcg cactcatgtc gttttgagca tagtttttca ttgctcgggc tgcagccaac
     9361 gcaaaattcc ctcctgatcc gatagctaag ataccatcat ctggtgtgat aacttctcct
     9421 gtacctgaaa caagaagcat ttctttatca ttcattacga tcagcatcgc ttcaagtttt
     9481 tgcatagatt gctgtgtccg ccattcttga gctaattcga cagctgcgcg ttgtagattc
     9541 ccgttatatt cgtttagttt gccttcaaat ttttcttcta aagtaaacgc atctgcaaca
     9601 cttccggcga aacctacaac aacttctcca ttgtagatac gtcgtacttt ctttgcagtg
     9661 cctttcatga caacagattc acccattgtt acttggccat ctccagccat agcaaatttc
     9721 ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg gtgattcgac catgtttgtt
     9781 tcctcctaaa tttgttcttt tccttatttt tacgctcttg gatggaaaga acgatagttt
     9841 ttttgtaaac tttcttttgt tacatgggca tagatctggg tagtcgataa attggcatgt
     9901 cctagcaatt cttggactgt ccgcaaatct gccccattat ttaataaatg agtagcaaat
     9961 gtatggcgca gcatgtgagg atggatatca ctatccaaac tgcttttttt gatcatttga
    10021 ttcaagacat attcaattcc agtaggcgtg attggttttc catgatgatt cacaaaaacg
    10081 atatcatgct cttcgtgata ttttgtcatc aatacctggc ggccttccct gaaatattct
    10141 tgcagagcat cttgagcaaa agaacctaga ggagcatatc tctctttgtt ccctttacca
    10201 tggatcaaca tgacctctga atcaaaatca atgtctgata ccgacaaact gctacactca
    10261 cttaaacgga tgcctgaccc gtaaaggact tccagtaaag cgcggttcct caaatccaaa
    10321 ggactatctc ctgctacgct gtcaaaaagt gcttgcattt cattttcata aaaaaaacgt
    10381 ggcagtctca aatttttctt tttgagatga acgtaagaaa atggattttc ttttatcact
    10441 tcttctttga gaagatactg ataaaacgaa cgtaggctgg ctatcttgcg actgatagaa
    10501 tttctgctat agctaggacg atgatcagct aatgcactca aatagacacg aaccgtaaaa
    10561 tattccgcct tcagcagatc agcgtctcca gattcctcca aaaaatgttt gaaatggaga
    10621 atgtcttctt catatgcttc tttcgttttt tctgaatatc ctctttcgac aatcaaataa
    10681 cgcaaaaatt tttgttccca cgtggcttct gtcattttct caccttcctt ttgtcccaga
    10741 gaataattta gcacagcgtt tataaaaaga caaataaatt gtcagaattt aaccaatatt
    10801 tctaagtttg ttcacaattg gcgcaattcc tcacattttg tttgagaaat tcttaagaaa
    10861 aacgcaagaa aaagagcgag actatccgtc tcaccctctt catggagttt ccttactcat
    10921 ttgggattgc agttttttct aattctgttt ttattgcctc attagctttt aaagctcgtt
    10981 gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg ctctggcagt tcaggaaaca
    11041 gaccatagtt ggcattcatc ggttggaaat gttttccttc cgcatgagta atgtagtatg
    11101 ccatacttcc aatagctgtt tcttgtggaa actcgactgg ttccatttct tttgctaagc
    11161 gtgcggcgtt gattccagca agtagtccgc tcgctgcact ttcaacatag ccttctacac
    11221 cagtcatctg acctgcaaag aacagatctt cgcgtttcgt tgattggtaa gtcggtttca
    11281 gcaattctgg cgaattcata aagctgtttc ggtgcatcac accatagcga acgaattcag
    11341 cattttctaa cccaggaatc atccggaaaa cacgtttttg ctcgccccat ttcaagtgag
    11401 tctgaaagcc cacgatgttg tataaagaag ctgcagcatt gtcttgacgc agctggataa
    11461 cagcataagg acgtttgcca gttttaggat cttccaatcc aactggtttc ataggtccga
    11521 aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat tggcatacat ccttcaaaat
    11581 atttttcttt ctcaaaagat ttaagttctg ccacttcggc attgattagt gcctcatgga
    11641 aagctttgaa ttcctcttct gtcatcggac agttcaagta agctgcttct cctttgtcat
    11701 agcgggactt caaatatact ttatccatat cgatcgtagc tttgtctacg attggtgcag
    11761 ctgcatcata gaaataaaaa ccatctgaac cattgaatgc tttgatgctt tctgctaatg
    11821 gatgagaagt caaaggtcct gttgctacta cggtgattcc ttccggtatt tcagtaattt
    11881 cttcgtttat cactgttact aatggatgct ctttgacttt tcgggtaatt gtttcagaaa
    11941 aagaatcgcg gtcaacagct aaggcaccac cagctggcac tgctgtttca tctgccgaag
    12001 tgattactac cgaatccaat cggcgcattt cttctttcaa taaccctact gcattagcca
    12061 aactgtttcc tcttaatgaa ttggaacata ctagttcagc aaaattactt gtgtgatgcg
    12121 cctcagtcga tttgactgga cgcatttcat ataaacggac agggactcct gcttgtgcga
    12181 tttgccaagc agcttcactt ccggcaagcc ctgctccaac aacatttacc attttcaatg
    12241 aaagaacctc ttttctatta tttttggatg tcttcttcgt aatctccatt tacacagact
    12301 acttgtttac cgcctttgac tttcttctcg accagatatc cgtcacattt tggacaattt
    12361 ctgcccactg gtttatccca agaagtgaat tcacattctg ggaaacggga acaaccatag
    12421 aatagtctgt ttttcttaga ttttcgttca atgacttgtc cttcatgaca tactgggcaa
    12481 gtcacaccga tttcttttac gattggtttc gtattccgac aatcaggaaa attactgcaa
    12541 gcataaaact tcccatattt ccctaatttt attaccatcg gatgtccaca gacatcacaa
    12601 tcgaatcctg ctggttcatc tttgatttga attttttcga ttttttcttc cgcattcgtc
    12661 agttcttttt caaacggttt atagaaacga tcaaccactt tgacccacgc ttccgtgcct
    12721 tcttcaattt tatccaaatc tccttccatt tctgctgtga aatggatatc gacgatttga
    12781 gggaagaatt cacagatcag tgaattgaca atttctccta gttcagtcgg ctcaaatctt
    12841 ttttgtgtca attttacata gtagcgtctt tggatcgtat caagtgttgg tgcataagtg
    12901 gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca acgtagcctc actaaaacgt
    12961 gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa cagattggac tatatcgcct
    13021 tcttcaagtt ctggaagaat attttctttc tcttctttgc cgtcatctct accttccaca
    13081 tacacttgca tgaatccttt gaatttgatt tttgaaccgt tcgcaatgaa gatgacacca
    13141 ttttgttcta atgtcacttt cattgtatct aaaatagctg gtgtcatctg actagccaca
    13201 aaacgtgacc agatcaatgt ataaagtttc aattgatctt tatctaaata ttgtttgagt
    13261 tcatctggtg tacgaagcac actcgttgga cgcacagctt catgggcatc ttgtgcgcct
    13321 tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt cttttccata cgtatcttca
    13381 ataaatgctg cggcctctgc ttttgctgta tcggcaatcc gggtagaatc agtacgcatg
    13441 tatgtaatca agccgactgt tccttgttta cccaaagtga ttccttcata taattgctgt
    13501 gctaccatca tggtttttct tgttcgaaag tttaacttac gggcagcttc ttgttgcagg
    13561 ctacttgtag tgaaaggatt cgcagggttt cttttgcgct ctttcttttc tactttttta
    13621 acttcgaagt ctgttccatc aattctagca atgacttctt ttacactttg tgcatctggt
    13681 aatttctttt tcttaccgtc gattccccag aaatttgctt tgaatttctt gcgagctttt
    13741 ttaaaattac cgtcgatgct ccaatattct tctggaacaa attctcggat ctctttttca
    13801 cggtcaataa taatttttaa agcgacagat tggacacgtc ctgcacttaa ccctttcttg
    13861 accttacgcc agagaatagg actgatcgaa taaccaacga ttctgtctaa ggtacgacga
    13921 gcttgctgtg catctactaa atctacatcg atcgttcttg gttccttaaa agctgctttg
    13981 actgcgtctt ttgtgatttc attgaagacc acccgatttt tttctttcag atccaaccca
    14041 agtaggtacg ctaaatgcca agcaatcgct tctccttctc tatccgggtc acttgccaag
    14101 taaacttttt gagctttttt tgctgcggct ttaaggcttt tgatgacatc gccttttccg
    14161 cgtatagaaa tataatgggg ttcataattg ttttcaaaat cgatacccat tttacttttc
    14221 ggtaaatctc ttatatggcc aacactggcc attactttat agtttcgtcc aagatatttc
    14281 tcaattgtct tggcttttgc aggtgattct acaatcacta aatatttata cgccattact
    14341 gaatggactc ctttccattg tcgtgttcct actattaaaa gaggattcat acaaatcgta
    14401 gctaatcata tccattcaga aaacgtttgt caagaaaagc aaatgaaaag tatggtgtta
    14461 ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg caaactgcac cgtcttggat
    14521 caaatgatga catccggtag aatgttctgt taaaatattt cctggaacag aaaaaactgt
    14581 tcttccaaga tccaatgctt gctgtgctgt tatcagagag ccgctttttt tcttggcttc
    14641 aaagacacat gttcctttag acaaagctgc gataatccga tttctcattg gaaaacgatg
    14701 ctttttcggg ccggattgag gaggatattc actcaaaaga agatgatcat ttgcaatcac
    14761 ctcttgcaag tcacgatttt cagcaggata gacttgctct atccctgttc caatgactgc
    14821 gattgtccgt ccaccatgag agattgctgc ttgttgaccg tatgtatcta tccctttcgc
    14881 gcagccactg acgatcacat atccagcttc taccactggt tttagtaaat aataaactgt
    14941 ttttttggct aaagcagaag tgtgtcttcc accaacaacg cttaacatgt tacaagaaag
    15001 taaagataga tcaccattat aaaacagtac aattggcgga taggtcaagt gaagtagctg
    15061 agggggatat ttgggatcaa agcaagtgat aaacgactgt ttcgattgga ttttgcccca
    15121 gttagcgtga atttcctgcc aacttttgcg aaacagctcc cgatgtgtgg ttattttacc
    15181 tatcttaatg atgtcttcaa atccgatttc tttataagga tactgcatca tccagttagc
    15241 aagtgcccat tttccttgac tactgattcc ttttgcataa gcaagtttca ataaattctc
    15301 ttctatttga tacatcataa aaccgccttt cgtctctctt tctaataaga tacgtaaaag
    15361 cggctgtttt ttattgtaaa tattttttta caggtgcaaa acttttacga tggattggcg
    15421 taattccttg tcgttcaagg ccttctaagt gttcctttgt tccataacct gcattatgcg
    15481 agaaaccata tccaggataa agcacgtcat attctttcat caattggtct cgatatactt
    15541 tagcaatgat acttgcagca gcaatcgaca cagatcgtgc atcccctttg attatttttt
    15601 cctgctggat aggcaaatcc aaggtcatcg catcgatcaa caaatagctt ggtacaatgg
    15661 acaatttttc tactgcttgg atcatcgcta atttacttgc ttcataaata ttcacatcat
    15721 cgattgtctg ctgatcaacg atcccgatcc cgattgccag ggctttttct tgaacttgtt
    15781 gataaagaag ttctcgcttt ttctctgata attgtttgga gtcattcagc cctaatatga
    15841 tctcattttt cggcaagata actgcagctg ccacgacagg tccagctaat ggtcctcttc
    15901 ctacttcatc aatgccggca atccattggt gaccttgtgc gtacccttgc ttttcaaagc
    15961 aaaacatttc ttcatatttt tccttaatta atgcttcctt tttcaatcgt cgttcaaaag
    16021 aagccagtgc ttgctgcacg cctttgcgtg gatcatttct taatgattct agtgtaggat
    16081 catctattct ctgtatgctt ttcaatcgtt ctttgatact ttggatggat tcagtcatca
    16141 cttttttgct ccatttcttc gcaacgatcc agtgtgtatc gtccaagttt tccttggcga
    16201 atatcatgca cgactaattc gctgccgcga tcatagtctt ctttataccc acgttttgct
    16261 gtaatatcca tcagtaattc cggtgccaat ttttcttctt gctcgattga taaaccataa
    16321 cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt ctaatccgta aatcgctaaa
    16381 tcatctaaat ggacaagctg gtctttgatc gcgcctgtca gcgctagttt tttgccgatt
    16441 tcttcatctt caaatttggg ccataaaatc cctggtgtgt ctaacaattc caactcatta
    16501 cctaaacgaa gccactgctg accttttgtt acgccaggct tatttcctgt ctgtgcaatt
    16561 ttcttgccta ctagtcgatt catcaacgta gatttcccaa catttggaat acctaagacc
    16621 attgcccgaa tagcgcgagg cttgatcccc cgagcagcat ctcttgcccg tttttctttc
    16681 aatgcttctt tagccgcagg tactagacct ttaattcctt tactttcttg tgccgtgata
    16741 gctaatgcaa gatggccttg tttacgaaaa tattcttgcc acagctttgt ctgttgtgga
    16801 tctgccagat ccgctttatt caataaaaca aggcgaggtt tttgctgaat gatctgatct
    16861 agcattggat tacgagaaga taaaggtaat cgtgcgtcta ccaattcaaa tacgatgtcc
    16921 acatatttca ttttttcaga aacttctcgt cgggcttttg ccatatgtcc cggaaaccat
    16981 tggatggtca ttgttctctc cattcctttc ataccggaat attccccggc tttacttgtt
    17041 ttttttcttt ttcaatataa attcactttt ttattataaa gaaattccat ctattagtta
    17101 agtgaaagtg aaccagtcct cattctatca catcttcact tttttgatga tgtaaatttt
    17161 tattttggtt tctatatatg aatctaaatt tttgacaacg tttcctaaat aagagatgat
    17221 agacgaacaa aacagaaagg agacgaaaca aatgaatagt atggtttttg ttaattttcc
    17281 tgttgctgat gtcaatcgtt ccgttgcttt ttatgaaaag ctgggtttta aaaaaaatga
    17341 agaattttct actgaagaag ctggttcaat ggtatgggat gacacttttt gggtaatgct
    17401 tctctctcat gatttttata aaaaatttat aaaagataaa gaaatagctg ataataaaaa
    17461 aacaagtggt gctttgattt cgtttagtgt tgaaaacgcg gatgaagtaa aaaaatttgc
    17521 tcaaattgca aaagaaaatg gtggtacttt ccacgaagta gatatgggaa tgcctgaaga
    17581 agaaatgttt tcattagaag tccaagatct agacggaaat acattagaac ccgtatggat
    17641 gaagatgtaa gcagataact aatctgacgt tttttataac aaaaaaacaa aggcggatga
    17701 tacgataagt attctccgcc tctatttttt tcattcgtac aatctctttt ttacagttta
    17761 taaaagcagt cttctcaccc tttttttaat tgttagatgc cagtgtatgt aaatcaccaa
    17821 tgtgtcatga cacattggtg attttatggt atccttttat tattaactga caaaggagag
    17881 aatattttga acactttgat tgcaaaagac gtttttagat tgcccgaaat gaatgcaaga
    17941 ttgttgtcga cctctgctct tttattagcc cttgatgtca tcttatacaa gatagcgatt
    18001 ggtcctgctt attttcaaat cacattcgga tttttaagta tggcgcttat gggttatcta
    18061 tatggaccga tctgggctgg tttgcttgaa atgttgtcga acactttaaa tttcacgata
    18121 tttggttcag gaacgtttca agctgctttt cttatcacgg ctttccttgg tggatttatt
    18181 aatggcttat ttttatatca taaaaagata aacctgcctt ccgtcatcgc tgcccaatta
    18241 ttgattatga tcccagtcag cctttttatc aattcttggc tgatgtctgt tttgtttggt
    18301 gcagatttca aagtgatttt tctaggtaga gtcatcagga atatgatcca aatccctatt
    18361 caaataatgg ttgtttattc atttatcaaa actttagagg ctagaaaagt gtttaaacga
    18421 gctgtttgat tttacgtact aaggtagtaa actgattttg gattaaacaa taaagcgtgc
    18481 agtctcaaca gattgcacgc tttattgttt ctcactggat ccattttatt tcatcaagtg
    18541 gataatagac aaatcttgct ttacctagaa tatcagtttt attgatcgtg ccaaacgaac
    18601 gactatcttt tgaaactctt cgattatctc caagtacaaa ataattatct tccggaagct
    18661 tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt ataggatgca tttgcatgat
    18721 ctgatttcag gtttttagtt agataaggtt ccttaactac tttcccattg atcttcaatt
    18781 gatcattttg gtagctgaca ttttctcctg gaagaccgat tactcgtttg atataaatcg
    18841 tgccatctgc taattgaaaa acaacaatat caaatcgtct gatctccgaa aatttttcca
    18901 tcacaaccat atcaccttgc ttcaacacgt tttccattga attgccttcc actggtaccg
    18961 ggatgaataa aaatccccgc aacatgaacg caaggaatac agaaacaagt aagtatttga
    19021 atacaagcca gaagcgatta atatagcgtt gtttttttgt caatctctat caatccatcc
    19081 ctcgtttttt cattctttct ttattataag aaaaatatca aaaaaatgcg actagatagc
    19141 tgaaataaaa ataattcttt caattaggct attggacaaa aaactttttt gatcataaag
    19201 tacaaaacta tctcttatat gtcatgatta ttttctaaat atttttccca tgcttcgtca
    19261 aagatatcca tggacggcaa atagtcgaca ttcagttcca gataattaga aatttcttcg
    19321 tagctagagc tgtgcttagg aaattggatg tctttaccgg cttctgtagc gaaaacagat
    19381 aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat aatgaaaaaa gcttcgattc
    19441 acccttcgtt cctccaattt tcttcgataa aggcagcccg gcgtgcatgg tttcttttaa
    19501 aattttcagg atgcttttta taatactctt gatggtcttc ttctgctaga taaaatggtt
    19561 gggctggttc gatttttgta acgattggct gttcgaaacg tccactttca gctaactgtt
    19621 tcttactttt ttcagcaatg atccgctgtt cttctgtcaa ataaaagatc accgggcgat
    19681 aattgtcacc gcgatcctcg aattgtccga aagcgtcagt tggatcagtt tgctgccagt
    19741 aaagttctac taattgttca tatgaaatca atgttggatc atagataatc tccactgctt
    19801 ctgtatgtcc agtcgtttgg ctttttactt gttcataagt cggatctggt acatgccctc
    19861 cagtatatcc tgacatcact gtatggatac caggaagcgt gtcgaaaggc tggatcatac
    19921 accagaaaca gcctcctgca aatattgctc gttccattat gattccccct ctaatactac
    19981 tcatccgatg attttttatt ttcttttgtc ttgctttcag tactgctggt gctagttttg
    20041 ttggtatttg agttgtttga agcatcagag gtatcttttg gcaaatatag actaaaccga
    20101 atgtcatcat ttactaagtc gattttatct gctttaatat acatcccgtt ttgcattcgg
    20161 aattgatcca acctaagcat gatagtcaga tcatctgtat tgacttcgat ccaatctggt
    20221 aatttatagt ttctcttgat catattcatc acatctttga ttggcaaact caatgttccg
    20281 atagaaagac ttctagcttt tagttggaca tttccgtttt ccatcacata cggatcaaaa
    20341 tataaataga aatcgatagg aaaacctagt acttcgaaag taccattgag tagtgcttca
    20401 ttttccaaat aaaatttata ttcaatatca ctatcttttt gatactcact aaggaaaaag
    20461 ctgatgattt gattgacttt ttctttattt gaattgatcg ttaagacagg atctccttgt
    20521 ctttccgtaa gagctggtat ttctgggagt tctggttccc gattagcaaa aatacggctg
    20581 cctaaaaata cggtcgaacc aagtactacc ccaagaagga taaggaaggc gaatttccag
    20641 taatttgttt tctttgtttt gtttgaagat acgtcagatc ttgttttcat gtttattgat
    20701 catcctcctt ctctagccat tggttttgtg tctcgatcag ctgatctctc actgcatttg
    20761 ccatcaattg ataacctaaa ttatttggat ggaaatgatc ttcttcatag agcacattat
    20821 ttttgatgcc atttagattt tctgtcgtgc tagttgtttg atctgttcct tcttctgtga
    20881 cacctacttc ctgattcaat ccttgataaa gcaaatcatt gattgggata aagtagcagt
    20941 tcggcatttt ttttgtcacc gtttctgttc ctttgttcca attgttaaca attgtctgca
    21001 tgtcagtgat ctcaggaaag ttcaaataaa atggattgta gatgcctaaa atgtaaatag
    21061 gtgcggtttt attgagggat cgaaattctt ccaatagttc tgtgacattt tcctgatagt
    21121 ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa taaattattt tggataactt
    21181 tcatcaaatc attaccgcca acagtcagtg tgataaaatc agctgttttg atattgtttc
    21241 gaatttcttc ttttgttttt actcgcttta agatctggtc acttcgttca ccatttacac
    21301 catagttttc tgtttcaacc gcggtcaaat tataacggtc ttttagatca tttgcgacaa
    21361 ggggaacgaa tcctccttgc ttggtttgat ctcctactcc ttcagtcagc gaatctccga
    21421 tagctatgta gtgaagtttt tcttttttat ttggatcttc tctcacagta gttactggtt
    21481 tcattagtgg gccagctttc ggtatagaag cagctaaacc tgcatagatg atgatcgcaa
    21541 ttaataatgg gaacaaaacg aggatcagat gtcgtttcgt taatttcatt ttttcacctc
    21601 ttgtattcga ttggttctaa tgttatcaaa aattttatca aaaaggtagt ctttatgaaa
    21661 aaagaaaaca attattttcc taattttata aagaaaaaga gaataagctg caagcctttc
    21721 aggctgaggg cttatcctct tttcgactaa tctacataat acatgattgc aaacgcgtct
    21781 ttaccagtat gcgtagcgat aaccggattc gtatgcagta cagggatatg catgtccggg
    21841 aataattctt gcaaaccctc tttgaattct tgcgccaatt cttttgcatc cgcatgtgaa
    21901 ataccgattt gtcgcacatt tggcaattct ttcagttcat ttttcaaatt attaaaccat
    21961 ttatcgaacg ttttctttcc tcgcccctta gttacaggga ttaattcaga atgggcgaaa
    22021 ttcatcacca cgcgtatgtt caaaacacta gacagcaaac cagttgttcg actgattctt
    22081 ccacctttta ccaagttatc tagtgtggat attccgatgt ataattttgt attgtcttgt
    22141 acttttttga cttcttcaag gatttcctct actgttttac cttcttttgc taattcagct
    22201 gctcgaatta cctgaaaaga taatccttgg tcagtggtat cactgtcgat aaccgttact
    22261 ttcgtttttg ttagattact ggcttgtctt gctgcttcta ctgttccact caagcctttt
    22321 gtcatatgga tcgaaatcac ttcactgccg tcttctccta gctcatcata catcgagata
    22381 aattctccaa tcggtggttg gcttgttttg ggcagatttt ttgcttttgc catcatttcc
    22441 ataaatcttt cgccgtctag ttggtcatca tcgggatata ctgttccgtc gatcatgatg
    22501 gatagcggca tgacacgaat gcctaaagca tcgcgtacat ttttttccat cgtacatgaa
    22561 gaatctgtta cgatttttac atttgtcatt attttctctc tctctttctt taacaagatg
    22621 ccttattcat gttaaactac atgtaagaca atttctattt ttagtataac agacaaataa
    22681 tgatttttca ttaattacac ctagtaaagg agccgttttc atggaaaaaa cgaaatttaa
    22741 tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc acccatggaa ttgggactgg
    22801 attgagtatc gcgggactag tggttctttt agtaaaaggt gcccacttgc attcctccct
    22861 ccatattgtc tcttatgcta tctatggatc gatgatgatt cttttatttt taacgtctac
    22921 tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg ttccaagtat tcgatcatag
    22981 ttcgattttt cttttgattg caggaagtta tactcctttt tgtttgctga gtatccgagg
    23041 atggctaggt tggactttat ttgtattaat ctggttgttt gcgattcttg gcattatata
    23101 taaagcgttg acgttgcata aacgggagag agtatcaaaa gtatctacga tcatctatat
    23161 ttttatgggg tggctctgtg tcgtggctgc tgttcctctt tatcactctc ttggaatgac
    23221 tggtgttatt ttgatggtag caggcgggct ttcttacacg ataggcgcat tcttttatag
    23281 cttgaattct atccgctata tgcatgttgt ttggcatttg tttgttatgt taggtgcagg
    23341 ttttatgtac ttctctgttt tattctttac ttaataaaaa aaaataaagc tatggatggg
    23401 acagctctaa ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc
    23461 cgctttttac aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt
    23521 tttcatctat ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg
    23581 gaaaataagc atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa
    23641 atggcagaaa ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt
    23701 ctttttccag agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat
    23761 aggaagaatc tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc
    23821 cttcgaaagt ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt
    23881 tcagatcatt tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat
    23941 cctgtgccca aattgcagct aacattttct catcctttct gattctaaac agcgatagga
    24001 gctttgatcg ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca
    24061 aatactgact gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc
    24121 aattgttctt tcatttgttc gatatgattg atataaagat gagcgtctcc taatgtgtga
    24181 acaaattcgc caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa
    24241 cttgcaatgt tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag
    24301 cttagccgtc cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg
    24361 ctaggaacat cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt
    24421 ttgattgctt cgatcacttc tttgatttga tcgataaagc caccgtcttt tgtttcccag
    24481 tgacgccatt gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca
    24541 tcggttagga tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca
    24601 ggatctgtca aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt
    24661 ttgatgtagc gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag
    24721 cggatattcg tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt
    24781 actcgtttcg ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg
    24841 aataagctta atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat
    24901 tttcttccta agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt
    24961 cactcaagta ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt
    25021 cgttttgcaa ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga
    25081 gcaatgagat tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt
    25141 gttccatgta agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg
    25201 ctttttcagc aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt
    25261 aataaagttc ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca
    25321 agaaataacg gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt
    25381 ccaaaatcgt caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat
    25441 taggttgctg gatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt
    25501 tgcctatcag tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta
    25561 ctccgatttg actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa
    25621 tcatccgttt atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg
    25681 tttcaccgat agacattgta ccgctatcca ggggaattct gccagctaga atattaagca
    25741 atgtcgattt tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca
    25801 gtaaatctaa atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt
    25861 ccagtacttt tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt
    25921 ttacttggta caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg
    25981 tgccacgtgc ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc
    26041 gcttttcttc ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt
    26101 cataatttcc tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta
    26161 ctctatctag aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca
    26221 aatagttttc caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta
    26281 atagtaaatc gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac
    26341 ctgataaagt actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct
    26401 ttgcgttggt atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat
    26461 aacgtttttg cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat
    26521 atgctcgaac agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta
    26581 attcatcgga aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa
    26641 acactgtttg ccgatcaccg tcgccgctat cgataccggc aataatattt aagagacttg
    26701 ttttacccgt cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag
    26761 aaatctggtc aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt
    26821 ctttcaacat gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga
    26881 aaattttttt ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc
    26941 ctgttctgat atgttttgtt ttttataaaa cttgaaaatt tttacctttt tattttattg
    27001 gactcgtttt tttgactttg ctcgaaacgc aaatcggaaa gaaaagttat cagatggttt
    27061 gttcatcgta taagattcta acaaattgat attcctgcga tagctgttcg cagaaaacgt
    27121 cgtgattttc tcagcaactt cgtattcatc aatgattttt ctttcaagat aataacctct
    27181 tagcattttt tttgtaaaca gactatcttg ctggatcatc cacgaagaaa tcaaattccc
    27241 cgagttcatt tttattcctt caagtttgcg ttgctggata aaaaattgaa ccatcgtttc
    27301 atcataaata tcagtcaagt tatctaaact ttcaataatc aactgagtat ttttttgata
    27361 tgtctctctg atttcttgga tatcttcatc actaataaat gtctgacgat cagcatgtct
    27421 tctttgccaa aacattttcg gatggagaac gattcgcatt aacctacgga caacaatgaa
    27481 ccaaaagccg ataaaagaca aaatctggct tgtgatagaa tgttcgaatc gagaaagaaa
    27541 acgggagtaa aaacgataac cattagaaga aagcttgccc gaacgataac tttcatccaa
    27601 tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct tgtacttctt gcttttcatc
    27661 atcggttaaa tcttccaagt acagatcacg tatccgttct tgataatttt ctatcactgc
    27721 ttcagtggct aaaaattctt ttgtattacg atcaatttcc tttatttctt ttcgcaaaac
    27781 ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc gtgcttttcg cttcctctgt
    27841 ttccgtcagc ataggcaaga caatgatccc tatgaccaaa gtcactaaga tgacacccgc
    27901 cgtcagaaac aaaagcaaag accgctggta aaacatcata ttattgattg aaaatggcaa
    27961 aataaaaatc gtagccaaac tgactgttcc ctttacaccg ccaaatgtta agatcaagat
    28021 ctcgttcaac tgttttttga actttttact gccatctttg aatacataaa aaagactgat
    28081 agaaatgaat ctgatcacaa aaagcatgac agagattaat acgataatcg ctaataaaag
    28141 accgtttgga tataaaccgt ctccccatac tggcgagaac acttgcgtca attcgattcc
    28201 taaaaatata aatactaatg cattcagtgt aaaagcaata gttgtccatg tactgtggga
    28261 caagctcgac aattctgcat caaacacaga gattttccta aaaccagaag cttggagaat
    28321 acctgctgcc actgcagcaa tgattccaga tacatcaaat gcctctgcca atacataggc
    28381 tagaaaagga agcaaaagtt cgatcagcag ataccctgtg acatcttgtg ctgatgcttt
    28441 ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc aaaaagccga tcaaagctcc
    28501 cccaatacta gaaactacta gactcatccc cgcgtttact gctgaaaaag atccggtgat
    28561 cagcgcacca agagcaaact ggaaggcagt aacaccagat gcatcattca aaagaccttc
    28621 tccttccaaa atatgcatcg cctttttagg gatattcact cgtccagaaa gagaactgac
    28681 tgctaccgca tctgtcggac ctaacgctgc tccaaaagca aagcaggcag caagtgggac
    28741 acttggcaac aggaatgata acgtcgcacc taccccgaca agggtcaaaa tgactcctcc
    28801 aaatgccaag aacaaaattg tcccgaaatt tttgagaatc gaagaaatat ctgctttttc
    28861 tccttctcta aataacaatg gtgcaataat cataactaag aacaattcag gttcaaaatc
    28921 gattgattgt ccccactctg tcagacctaa taccactcca aggaaaattt ggatcagtgg
    28981 agctggaatc attggcaaaa tgcggctagc tatgttcgat aaggtgatcg ttactgcaaa
    29041 tacaataact aactcagcaa attccatcgt ccatcccctt tattttttat tttgtgtaag
    29101 catttttaaa aattgttctt ttttctttaa aattttggtt tcgactactt caacctgtgt
    29161 ttggttaccc tctaattcac actgtacacg ttttgactcc aaacgaagca gatcatgttt
    29221 cagacgttcc aactgacgat acatcaatat atttctttta cgcaacttac aaagtatttg
    29281 acggcggtca tccatcgaaa gggcgccata gcgatcatgc aacagttttt tttgttgttc
    29341 aaaactctct ctcattttcc agatctccta tacacaaact tccatcatta tagcaaagtt
    29401 ctttttggat gaaaaatatt tcattgtttc atcgtttatt gctagttaga aatttccata
    29461 aatttttcaa cgcctcattc tattccaaat agtatcagat atcaagtaaa acttaagaaa
    29521 gataaaagtt tctgacaaaa gaatgtttac ttttccattc ctgcctttct tgttctttta
    29581 tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac agtcccttat tctgaataaa
    29641 cggtgtggaa gagacagcct atcagtagta ataagacaca tctctttcac agcttcattt
    29701 ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac atcttctaca cgttgatctg
    29761 gattgatcac gatatacaag ttacctgttt tttcgctgat ttttgttgat tgatattgat
    29821 tgtctaatcc ttctgttcct ttatctagat atgggaagta cacaagatca ggtgtgccat
    29881 ttcggtaaag gatttccatt cgctcgatat cttcgtattt caatgcgcga ttaaacatgc
    29941 ccatttccag tccgcctagg ttgatatcat gtgtcgcaac tgcgtcccct tcatggaaga
    30001 tttcgatttt aaatccagca cacggatgga tttcaacaaa ttcactgcca ttgattcgtc
    30061 caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat atgacggcgg ttgatcgtcc
    30121 aggtttctcc atttctaaaa taaaatttta gcgctgtcat ttctttactc attaaaaaga
    30181 ctcctttatg atgtaattta ttcttgtact acctcagttt agcacaaatt tatgtaagtg
    30241 gtcacatctt atgttttttc ttctaatgac gtatctgccc actttaagta gccaacaaag
    30301 ctgagcagat acagtctagt gttttttaaa tcaatttttc tttcgataaa aaatccaaaa
    30361 cctgtttttt atcatttttt attctttgtt ctacgacggc tttttcagct aattgcaaag
    30421 cttcactaac ccacttacca ggtttttttc cagtttcttc tagcaagatt ttaccatcta
    30481 ctgctaattc gcttaatgac ttgataggta aacgatcata ggattctcct acggcatcca
    30541 tctgaggtgt ttcgtcaaaa tagatcatca attcttctac ttgcagtgca tgttttttgc
    30601 ctaagaagta caattcgaat ggttccaaag ctccagtttc tcgttgtcgt agtccttgga
    30661 caagtgcttg aacgtcttta atcatttggt tagaacactt ccacgatttc aaaaaggaac
    30721 gaatctcatt ttcttttaga cccagttggc tgatcaataa tgtccatact tggctctctt
    30781 ctttcaatgg gacggatggt aattcagcaa aacgtaaaag tgcttcgcct tcttctttca
    30841 aactgggaca gcaaatgtag cactctgttt caataaatgc atggattccc gcggcccgtt
    30901 catttcccaa gagcatcttg atgaattcca catttatacg ttctacagat attttaccta
    30961 atagctcgtg gttttctttg atggcgtaga aggtttcttc ctctaatttg aaccctagtt
    31021 gactgacaaa acgcaacccg cgcatcattc gtaaggcatc ttcatggaac cgctcatgag
    31081 gattgcctac agctcgcaag atctgttctt ctaaatcttg cagaccatcg aacaaatcga
    31141 tgatttctcc atcttctttc aaagcaaacg cattgatcgt aaaatctctt cttttcaaat
    31201 cttctgccaa cgaacgaacg aattcgacat gatctggtct gcggtagtcc tgataagttg
    31261 attccgtccg aaatgtggtg atctcatatt gttcatcatt ttctaatacc aacacagttc
    31321 catgttcgat cccaatatct atcgtttttg gaaaaagctg tttgatttct tctggaaatg
    31381 cgcttgttgc gatatccaca tcatggattg gctggttcag caagatatca cgtacacttc
    31441 cacctacaaa ataagcttca tatcctgcat tctctaattt tttcagaact ggaattgcct
    31501 tttggtattc cagaggtaat tgttttaatt tcatttttcc aacctttcct gcgtgtttct
    31561 tgtactgaga taaagactca gttgcatggt gactgtttga caagtccgtt tcagagtctt
    31621 caaggccagg accacttcat cttgtttact cgtccttacg ctcaaagcga ttatggtctc
    31681 tttgattgaa tttttccatg tttttttcaa aaacttcagt taagtcgata ttcaatgaat
    31741 tagccatgat catcgtaacg aataaaacat caccaagttc ttctgctacc gagttaactg
    31801 gttcagaagc ctttttgctt ttttctccat aagtgtggtt gacttctctt gccaattcac
    31861 ctacttcttc agttaatcga gccatctgag ctaatggaga gaaatacccc accttgaatt
    31921 gctggatata ttcatccact tcctgctgca tactgtataa agaacgcttt ttatccatct
    31981 ttttccctcc aaaactatct taccaaaact ttaacgcaaa tcctagataa gagcagttga
    32041 gaaaatcgaa tttccatgct acatttagaa aggaatttct aaatggaaat tctgtgaaaa
    32101 tacataattg aaagctggaa ttcatatggt caaacgaaaa acagtaaaag aagttctttt
    32161 tatcattata ggaacaagta tttatgcatt tggtcttgtt tacttgaaca tcgctaatca
    32221 tttagcagaa gggggcgtct cagggatcac cttgatctta agagctttat tcggaattga
    32281 tccagcttat acgactttat taatcaacat cccattgatt ttactaggag gaaagatcct
    32341 cggtaaacgt tccttggcct atacagttct aggtacactt tctttgtcat tctttttatg
    32401 gatctggcaa agggttccac tagaaatcaa tttacagcat gatctactga tcgtttctct
    32461 acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc taccgcatgg gtggaacaac
    32521 aggtggcagc gatattattg ctcggatttt agaacaaaat tacggcatca ctatggggcg
    32581 ttccctattg gcatttgata tcatcgtctt acttgcttca ttaacctata tcgacttgaa
    32641 acgtatgatg tatacgttga ttgtttcgta tgttttcagt cgtgtgattg attcgatctt
    32701 agatggcgga tattctgcaa aaggtctact tgtcgtctcc aacaaaagcg aagagattgc
    32761 accactactg atgaccggtc tgcaaagagg tgtgactttt ctttctggag aaggcgcatt
    32821 ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc tctagtgaat taactgaagc
    32881 taagaggatc gtccacgaga ttgacgaaaa agctttcctt tcaatcatca atgtgcatga
    32941 agtggaaggg caaggcttta cttatttgaa gccacagaca agattactga aaaaactata
    33001 agaaaaaaga caaagatagg ctatgaggaa a
//
LOCUS       DAPBAB010000025        32689 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPBAB010000025 DAPBAB010000000
VERSION     DAPBAB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.025"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            189..419
                     /locus_tag="RD444_001456"
     CDS             189..419
                     /locus_tag="RD444_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDT7548839.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="RD444_001457"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="RD444_001457"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDT7548840.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="RD444_001458"
     CDS             1830..2006
                     /locus_tag="RD444_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDT7548841.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="RD444_001459"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="RD444_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDT7548842.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="RD444_001460"
     CDS             2622..3089
                     /locus_tag="RD444_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7548843.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="RD444_001461"
     CDS             3174..4058
                     /locus_tag="RD444_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7548844.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="RD444_001462"
     CDS             4172..4408
                     /locus_tag="RD444_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDT7548845.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="RD444_001463"
     tRNA            4496..4576
                     /locus_tag="RD444_001463"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="RD444_001464"
     tRNA            4583..4654
                     /locus_tag="RD444_001464"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="RD444_001465"
     CDS             4779..4949
                     /locus_tag="RD444_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548846.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="RD444_001466"
     CDS             5169..5876
                     /locus_tag="RD444_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7548847.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="RD444_001467"
     CDS             complement(5935..6579)
                     /locus_tag="RD444_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDT7548848.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="RD444_001468"
     CDS             6705..6992
                     /locus_tag="RD444_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548849.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="RD444_001469"
     CDS             7100..7420
                     /locus_tag="RD444_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7548850.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="RD444_001470"
     CDS             7501..8814
                     /locus_tag="RD444_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDT7548851.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="RD444_001471"
     CDS             8811..9530
                     /locus_tag="RD444_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDT7548852.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="RD444_001472"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="RD444_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDT7548853.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="RD444_001473"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="RD444_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDT7548854.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="RD444_001474"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="RD444_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDT7548855.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="RD444_001475"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="RD444_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDT7548856.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14967..15860
                     /locus_tag="RD444_001476"
     CDS             14967..15860
                     /locus_tag="RD444_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7548857.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="RD444_001477"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="RD444_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDT7548858.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="RD444_001478"
     CDS             17797..18654
                     /locus_tag="RD444_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDT7548859.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="RD444_001479"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="RD444_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDT7548860.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="RD444_001480"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="RD444_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDT7548861.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="RD444_001481"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="RD444_001481"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDT7548862.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /locus_tag="RD444_001482"
     CDS             21410..22168
                     /locus_tag="RD444_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDT7548863.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="RD444_001483"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="RD444_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDT7548864.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23095..24126
                     /locus_tag="RD444_001484"
     CDS             23095..24126
                     /locus_tag="RD444_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDT7548865.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24123..24890
                     /locus_tag="RD444_001485"
     CDS             24123..24890
                     /locus_tag="RD444_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDT7548866.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24990..26558
                     /gene="liaX"
                     /locus_tag="RD444_001486"
     CDS             24990..26558
                     /gene="liaX"
                     /locus_tag="RD444_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDT7548867.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26855..27175
                     /locus_tag="RD444_001487"
     CDS             26855..27175
                     /locus_tag="RD444_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDT7548868.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27175..27525
                     /locus_tag="RD444_001488"
     CDS             27175..27525
                     /locus_tag="RD444_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDT7548869.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27692..28630
                     /locus_tag="RD444_001489"
     CDS             27692..28630
                     /locus_tag="RD444_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDT7548870.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28645..29475
                     /locus_tag="RD444_001490"
     CDS             28645..29475
                     /locus_tag="RD444_001490"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDT7548871.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29519..30544
                     /locus_tag="RD444_001491"
     CDS             29519..30544
                     /locus_tag="RD444_001491"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDT7548872.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30559..31497
                     /gene="galU"
                     /locus_tag="RD444_001492"
     CDS             30559..31497
                     /gene="galU"
                     /locus_tag="RD444_001492"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDT7548873.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31622..32062
                     /locus_tag="RD444_001493"
     CDS             31622..32062
                     /locus_tag="RD444_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDT7548874.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32065..32667
                     /locus_tag="RD444_001494"
     CDS             32065..32667
                     /locus_tag="RD444_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7548875.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        10852 a         5544 c         7087 g         9206 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaagcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgac gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagtttc aggaggtctc tttgacttag atcagctgga agaagacatc gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgacagcga agccgcacaa aaagtgatca
    13261 gtgaaaataa cgctaataaa gaaacctatg accaattcca ttctcttgcc gaagaactag
    13321 aagaaatcga attgatggtg gagatgctgc aagaagaacc tgatgcagca atgcaagaag
    13381 aagcagaaac acgtatcaat gcattagatg aaaaaatgaa gacgtatgaa ttatctatgc
    13441 tgttagacgg accttatgat cgaaacaatg cgatcgtcga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagactgg ggcagcatgc ttttacgtat gtatacacgt tgggcagagc
    13561 agcatggcta tcaagtagaa acactagatt atcaagcagg tgatgaagca gggatcaaga
    13621 gtgtgaccct gctgattaaa ggacataacg cttttggtta tctaaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacggatt tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gctcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatcccta ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct cgattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gagatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tcgtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacgttgact ttagtcggtg tattcatggc agttatcatg aatgcaacta
    15121 aattagctca ggatatagaa ggaaacgttg atgtatctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagtaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaggaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat ggctgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaagcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaaatc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatatctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactgatt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcatttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacacaaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgaggattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgtg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta ttgttgccac accatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    19321 aaggcagtac cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatgtagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctgacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcattgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggctttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcggctcg tatcagcgac tatactgcct ttttctatct
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cgggagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc atatttttca
    22981 tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc gggaaagggt
    23041 ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    23101 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    23161 catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg aataaaagag
    23221 gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga atcgatttat
    23281 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    23341 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    23401 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacatggt
    23461 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    23521 tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    23581 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    23641 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatctttgg
    23701 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    23761 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    23821 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    23881 ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    23941 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    24001 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    24061 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    24121 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    24181 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcttgt
    24241 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    24301 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    24361 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    24421 tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta gataagactt
    24481 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    24541 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    24601 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    24661 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    24721 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    24781 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    24841 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    24901 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    24961 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    25021 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    25081 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    25141 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    25201 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    25261 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    25321 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    25381 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    25441 tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    25501 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    25561 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    25621 aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc caaactgttt
    25681 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtc cctgggattg
    25741 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    25801 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    25861 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    25921 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    25981 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    26041 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    26101 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    26161 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    26221 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    26281 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    26341 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    26401 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    26461 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    26521 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    26581 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    26641 cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga acttgccaat
    26701 tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat gaatatggta
    26761 atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt cacaacgatt
    26821 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    26881 caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    26941 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    27001 cattttatac attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta
    27061 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    27121 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    27181 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    27241 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    27301 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    27361 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    27421 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    27481 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    27541 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    27601 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    27661 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    27721 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    27781 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    27841 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    27901 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    27961 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc
    28021 tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca gttatttaga
    28081 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    28141 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    28201 acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    28261 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    28321 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    28381 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    28441 cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg tcaaaacagg
    28501 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    28561 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    28621 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    28681 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgccatg
    28741 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    28801 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    28861 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    28921 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    28981 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaagcaatc
    29041 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    29101 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    29161 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    29221 tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg ttatctaatg
    29281 tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta
    29341 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    29401 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    29461 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    29521 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    29581 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    29641 caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg attccatcca
    29701 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    29761 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    29821 gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct ctgacatctt
    29881 gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    29941 tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    30001 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    30061 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    30121 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    30181 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    30241 aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    30301 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    30361 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa tggaaatcga
    30421 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    30481 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
    30541 ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc aggattagga
    30601 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    30661 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    30721 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    30781 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    30841 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    30901 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    30961 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    31021 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    31081 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    31141 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    31201 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    31261 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    31321 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    31381 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    31441 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    31501 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    31561 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    31621 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    31681 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    31741 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    31801 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    31861 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    31921 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    31981 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    32041 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    32101 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    32161 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    32221 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    32281 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    32341 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    32401 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    32461 agtattgcta aagatacttc agaagatatc ataattgatg taaaagacgc tgctggtaaa
    32521 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    32581 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    32641 atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaa
//
LOCUS       DAPBAB010000026        32573 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPBAB010000026 DAPBAB010000000
VERSION     DAPBAB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.026"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..626)
                     /locus_tag="RD444_001495"
     CDS             complement(<1..626)
                     /locus_tag="RD444_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7548876.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="RD444_001496"
     CDS             899..3370
                     /locus_tag="RD444_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7548877.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="RD444_001497"
     CDS             complement(3473..5209)
                     /locus_tag="RD444_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDT7548878.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="RD444_001498"
     CDS             complement(5206..5910)
                     /locus_tag="RD444_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7548879.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="RD444_001499"
     CDS             6041..6544
                     /locus_tag="RD444_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDT7548880.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="RD444_001500"
     CDS             6504..6842
                     /locus_tag="RD444_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDT7548881.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="RD444_001501"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="RD444_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDT7548882.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="RD444_001502"
     CDS             8393..8971
                     /locus_tag="RD444_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDT7548883.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="RD444_001503"
     CDS             8975..9496
                     /locus_tag="RD444_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7548884.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="RD444_001504"
     CDS             complement(9575..10129)
                     /locus_tag="RD444_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDT7548885.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="RD444_001505"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="RD444_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDT7548886.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="RD444_001506"
     CDS             10669..10920
                     /locus_tag="RD444_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDT7548887.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="RD444_001507"
     CDS             11045..11836
                     /locus_tag="RD444_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548888.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="RD444_001508"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="RD444_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDT7548889.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="RD444_001509"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="RD444_001509"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDT7548890.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="RD444_001510"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="RD444_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDT7548891.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14019..14312
                     /locus_tag="RD444_001511"
     CDS             14019..14312
                     /locus_tag="RD444_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDT7548892.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            15155..17482
                     /locus_tag="RD444_001512"
     CDS             15155..17482
                     /locus_tag="RD444_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7548893.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            17608..17937
                     /locus_tag="RD444_001513"
     CDS             17608..17937
                     /locus_tag="RD444_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDT7548894.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            17977..18981
                     /locus_tag="RD444_001514"
     CDS             17977..18981
                     /locus_tag="RD444_001514"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7548895.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            18978..20407
                     /locus_tag="RD444_001515"
                     /pseudo
     CDS             18978..20407
                     /locus_tag="RD444_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            20613..21785
                     /locus_tag="RD444_001516"
     CDS             20613..21785
                     /locus_tag="RD444_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7548896.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(21947..22402)
                     /locus_tag="RD444_001517"
     CDS             complement(21947..22402)
                     /locus_tag="RD444_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548897.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            22992..23414
                     /locus_tag="RD444_001518"
     CDS             22992..23414
                     /locus_tag="RD444_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7548898.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(23580..24773)
                     /locus_tag="RD444_001519"
     CDS             complement(23580..24773)
                     /locus_tag="RD444_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7548899.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            24997..25374
                     /locus_tag="RD444_001520"
     CDS             24997..25374
                     /locus_tag="RD444_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7548900.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            25362..25700
                     /locus_tag="RD444_001521"
     CDS             25362..25700
                     /locus_tag="RD444_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7548901.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            25834..26277
                     /locus_tag="RD444_001522"
     CDS             25834..26277
                     /locus_tag="RD444_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDT7548902.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            26539..27354
                     /locus_tag="RD444_001523"
     CDS             26539..27354
                     /locus_tag="RD444_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7548903.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            27364..28026
                     /locus_tag="RD444_001524"
     CDS             27364..28026
                     /locus_tag="RD444_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7548904.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            28231..29895
                     /gene="alsS"
                     /locus_tag="RD444_001525"
     CDS             28231..29895
                     /gene="alsS"
                     /locus_tag="RD444_001525"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDT7548905.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            29908..30618
                     /gene="budA"
                     /locus_tag="RD444_001526"
     CDS             29908..30618
                     /gene="budA"
                     /locus_tag="RD444_001526"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDT7548906.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            30748..31245
                     /locus_tag="RD444_001527"
     CDS             30748..31245
                     /locus_tag="RD444_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDT7548907.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            31430..31690
                     /locus_tag="RD444_001528"
     CDS             31430..31690
                     /locus_tag="RD444_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548908.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(32052..32291)
                     /locus_tag="RD444_001529"
     CDS             complement(32052..32291)
                     /locus_tag="RD444_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548909.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT        10964 a         5762 c         6524 g         9323 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaacgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt
    13861 ggtaggggag aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc
    13921 actatgataa tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac
    13981 ataaatggag tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc
    14041 aagaaaagat gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa
    14101 gttcaacaaa aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag
    14161 tgacttatac agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt
    14221 atgtcgaaca aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc
    14281 gggcatatca gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa
    14341 gttcaagtat gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc
    14401 tataatacaa agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag
    14461 cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa
    14521 tcaattggat gcgttacatg aataaagata attctctttt atttgtacga ataaatacca
    14581 aaacgtacct cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc
    14641 gttcttgaaa caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc
    14701 tgagcgttag aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga
    14761 atacaagatc agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg
    14821 acacatttct cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat
    14881 aatcccgtcc taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa
    14941 ttaatcaagt tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa
    15001 aataaaatct ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa
    15061 agccccaaac tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa
    15121 aggattattt gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa
    15181 taaaatccaa cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag
    15241 aaaatactta catcccatta tgatttcgat catcttgatt tttactttct tagggagctt
    15301 atcaagtatt tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga
    15361 ggagacaaat agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag
    15421 caacaacgag acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga
    15481 aatcacagca gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac
    15541 atttagtact gttgcggcca ctgcgaattt caacggacat accataccag atggatatac
    15601 agtgatatat gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa
    15661 aactgcgtta caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca
    15721 taatctgttt attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta
    15781 tatgggggtg gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag
    15841 ttattctttt acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt
    15901 ttcaatcggt atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg
    15961 gcagatcgtt gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt
    16021 tcaggtcaat gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa
    16081 aatcggaacg tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac
    16141 tccaacagca gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga
    16201 aagtaacgtc gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca
    16261 agactatttt ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc
    16321 tgcacaagac ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc
    16381 agcgacagaa acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg
    16441 aaatgtaatc gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa
    16501 gacaaatgca acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt
    16561 tgtaccgatc gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa
    16621 tgatctcagt attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa
    16681 gggagttgaa gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa
    16741 aagcggacta gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc
    16801 tgatgtctat acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata
    16861 tgatggaaaa gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc
    16921 gcttactcca gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc
    16981 aaaaaatatg gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa
    17041 agtcaaagca gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta
    17101 taccatctat aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc
    17161 ccaaagtcac tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc
    17221 attggattat accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat
    17281 tagtttaggc gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa
    17341 gttgacgatc aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt
    17401 tgtcttttta ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa
    17461 aaaagcaggt gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt
    17521 ctctttttta tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca
    17581 cctattacat ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt
    17641 caaaacgacg gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt
    17701 actttcgcta tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt
    17761 gtggaagacg cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa
    17821 aatgtgacaa aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa
    17881 gatgagcaag gtcgtgacgc tgtttaccga tttgcggaaa taaagacttc agattaagta
    17941 aaagaaaaat cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa
    18001 ttgacgacca tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta
    18061 acaaaaacag tagagaataa gcagacagat tttgcagatg gggataaagt tccttatctc
    18121 atcaccgtaa caattcctga gaacatcaac gagataggaa cctatacgat caaagataca
    18181 gctgatcctc agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag
    18241 attcatacat ttcacacaca aaaaacaaaa catggttttg tcttatcaga ttctggaaaa
    18301 gatcttagtt cgtttgctgg gaaaaaatta acgatccgtt accaaatgac attgaaagaa
    18361 aacagtgaaa aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta
    18421 gaaacacatc ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg
    18481 aaacaaaaac tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa
    18541 gaaaacggga taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc
    18601 agtaaagatg gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag
    18661 attaaaccgc ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa
    18721 ggcagttatg atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat
    18781 ccgacgaata atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa
    18841 aatggaggaa gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg
    18901 ttgttgctag tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac
    18961 aataggaggg aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg
    19021 ctatcatgct gcttcctttc gttctcggcc taggaactgc agaggtttct aggccgtatc
    19081 acctacacca gaaaatgtta cggttaattt gcataaattg aaattcactt cagcaccaga
    19141 aaatcaaata aacaacggaa cggaattgac atttccgaat agcgaaccgt tgaacggcgt
    19201 tgaattcaac gtatatgaca tcactgctac gtactatcca agtaaagaca cagcagttcc
    19261 agcagacgct acacccttcg caagtgtaac gacttctggt gaaggattag ctaacctgac
    19321 tcttccaggt aaatctgatg ggaaagatgc tgtatacgtc tttgtagaaa caccaaagcc
    19381 cggtgtcgaa acgtcgccaa atatcgtgtt aagcttaccc ttttataacc cgaattcatc
    19441 agaccctgac aaacctttag acacgattca cctatatgct aaaaatgtca tcaaagaaag
    19501 caatatcaaa atccagaaag ctgggaactt ttcaggggta gataagttag caggggcgca
    19561 attccagatc aaaaattcag agaatcaatt cgtgaccggt tttgatgaaa acactggtgt
    19621 cgctctctat gatcctgctg agtcagcgtt gacattcaca actgatactg aaggatccat
    19681 cagtgtcaat ggattaatag acggcaatta tactttagtt gaaacaaaag caccagatgg
    19741 ttatcaatta accaatccac aaaaagaaac tacttttata gtaaaaaacg gaaaattgca
    19801 acaaacaacc ggtatcatcc atggcgcaga tgaagccaat cttgggaatc caaacggtgt
    19861 agtcaatcgg gaaaatacaa ctacggataa cacgattact gtagaaaatg tccaaaacta
    19921 cggtgatttc cacttcatca aacaagatgt gaacacgcaa gaaaaattag ccgatgcaga
    19981 attttctatt ctaagagcaa aagatccaac tgttaaatta atgaaaaaaa tagcaaatct
    20041 tgcctcagga gaatacccat atgcttggtc agatgaagtc acaggttctg actgggaaga
    20101 agtgaaattg atttctgatt cagaagggaa attttcaatc gaagggctga aacaaggggc
    20161 atactgcctt caagaaacaa aagcaccaga aggctacgca ttacctaaga atgaagctgc
    20221 gtacactgaa tttacagtag atgatgatcc aacaacttct gatgaaacaa cgatcgataa
    20281 ccaacctaaa ggaatcttgc cttcaactgg tggtatgggg atcatcgtat tcatcttggt
    20341 aggtactgct ttagttggag gagcagtgat ctacttcaaa aaacgccatc aagaaacaga
    20401 agcttaaact attggaaaca aaaatcatac gataaataaa cgatagtgat tcaaatgggg
    20461 agaaaggttg tgacaagtgg tcggtctctt ggtattcgct caacaaacgg caaaatagga
    20521 ggaactattt tgccgtttgt tgagcgaata ctcaggtcaa ggcgctgatt tcacaacctc
    20581 ttcatataca tattagaaaa taggtggaca tgatgaataa aagcagaata agaatactgg
    20641 acatcttcat ggcaatcatt ttagtcgttg ggatcgggat cttttcttat ccttttgtag
    20701 aagattcact gaatgatttt cttgcccagc aaatgatcat ccattatcaa aagcaagcat
    20761 cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa aatgacaaag aaaaaccaac
    20821 agttggcaga gcaaaacgta tcaccaggga taggcagttt caaccaagca gttgatgcaa
    20881 aagcattaaa agatttacct agtaatgcct tttttatgga acatatgctt ggagtcatcg
    20941 aaatcccaaa aatcaatgtc agcttaccta tattcgatca gacaacggaa atatttttgc
    21001 aaaaaggaac gtcattattg gaagaaagtt cttatccgac tggcgggaaa aacacccatg
    21061 ccgttctttc tgggcatcgt ggactcccag aagcaaaatt atttacagat ttgcctaagt
    21121 tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa gacattggcg tatcaagtag
    21181 aaaaaatcca agtggtctta cctgatgaag tagattcttt gggtatccaa aaaggcagag
    21241 atctcgtgac tttgcttacc tgtactcctt atatggtcaa tacacatcga ctgcttgtga
    21301 cggggcatcg tattccttat caagcaaaag aggcaaaaaa agcgatccaa ggaattgatc
    21361 aatggaaaaa atggaaattt tttatttggt ttatcggtat actacttgga tcaatcggct
    21421 tagtttggct actcatcgct tatttagatt ctttggccat cgctaagcga aactatcctt
    21481 tatcttttta tgtaaagaac acaaacggac gaccaatcga aggaatggtc ttttctgtaa
    21541 aaacgctcaa cggcaaacac tatattacta gagaaaaagt tccatttgtg aaagcttctg
    21601 atgaatacgg actagtgaga ttttctgatt taaaaggcag gaactatcgc ttgcaacatg
    21661 aggagctcct gcttaaaatc catgtaaaac ataaacattc gaaacagttt tcaatgaaat
    21721 tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt gtactatttg attgagaaag
    21781 aatgaatgaa gatgtccaaa aaagcagagg gaacagacta aatgaaaaaa gagagaagct
    21841 gacgaaatgg acgtgtaaac tgctattttt ggtttactat ctggaacgcc agcttctatt
    21901 tttttatgtt tcagaagaat tttattatta tagtataact tttgaattag tttccataac
    21961 atttggagca aggggttaaa ccacgagctt gagcttcgct taaagtggca ggggtatacg
    22021 taccatttcc acatttatgt gtatggtatt ttgaaccggt cggtgtgaca tagacagttt
    22081 gttcattatt ttgctcagga gctgcggctt gtgcctgctg agaggcctgt tcagctgctg
    22141 cttgctgtcg cgcctgttct gcttgttgct gtgcgattgc ttcttgttgc gcttgttctg
    22201 cttgttgtcg tgcaatttct tcttgctttt tccgttcttc ttctgcttct tttgctttga
    22261 ttgtggaatc aattgcagca agacggcttg tcaactcttg tttgttttca ggtagcgctt
    22321 ggatagctgc tgaagcagtt gcatagtttt cttttgtagg atttgcttca gcttgtgaca
    22381 aagcagattc cgcttctgtc actttttttt ctagtgcagc tttttccgct ttttgtttct
    22441 cttcttcttt tttcttttca atttgttctt gttctttcga tgaatctgcc gtactttcgg
    22501 agatcgtggt actgctgctg gaagcctgag ctgaacttgt gctttgagta gtagacgtag
    22561 tagcaccaca cccactcaac aaacttcctg cgaccaaaaa caatgcaact aattttttca
    22621 ctaaatttcc tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt
    22681 acttacttgt tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg
    22741 ttttgataca cgggattttt tgctatagtt ctgttattaa gtatgcaata ataaaatatg
    22801 tacgttattt tattattaga ttattgacat ttatataata aacgattaaa tgattcatat
    22861 caatagaata cttaggaaaa aaagagaaat aataacaggt ttatgcacta ttgacggtgt
    22921 gacaatatga tatttgtatc cgtttttttt cttgtatgta gtatagtaaa gtagatacag
    22981 gaggagaaaa tatgcagact tttttaacaa aagtttctca aaagaaaatt aaaatgattc
    23041 atttactgct tgaaacagaa cgttggtgtt caatagaaga actacaaaac gaattgaagg
    23101 tatcaagtaa gtctatcttg aattatctaa cagatttaga agaattgttc caaaaatatc
    23161 ctgataaggt cattttgaag aatgagaata atcggcgctt ttctatggaa aagcaggaga
    23221 acttcccaat ctacattatt tatcttcatt tctacagaaa atcatataat tatcatttga
    23281 ttgaattcat gtatcaacat cctgagaaaa atttggaaga ttatgcagac gctcaatata
    23341 cgagtgtatc cacagttttt cgttatgcta agttattagt aaagtacttt gaaatgacgg
    23401 acgtcaaaga atagtagccg ccagatagaa ggaaattagc agttgaaaaa gctgccctgc
    23461 atcaagtaat acgaccagcc aaacggaaac agtgtctcat gtttttgatt tggctggtct
    23521 tattaccgca gatgcaagct tttgccacac ctctttcttg tcatttacag actcaactat
    23581 caatgctgca agctttccct taatgctgta acaccagtaa gagttgtcaa gaccagcaga
    23641 ctgactaggt aaataccgct caaaaacaag attacggcag ttaaactata atgatccatc
    23701 aagaaaccga tgacgataga agaaaaacca ccaacagctc gtccaaggtt cataatgaaa
    23761 ttattcgccg tcgcgcggat ttctgttgga tacaaactac taatcactgc gccatatcca
    23821 ccatacatgc cattgataaa gtagccgact atcacagcgg ctgctaccaa actccactgg
    23881 ctatgggcta acacaagtag atagacagat aaggatgaac aaattaaaaa caaagaaaag
    23941 gctagtttag gccccaattt atccatgatt atgccaaaag tcatcatccc caaagacatg
    24001 cctaaaatag tactgaccat ccacaatgaa gacccagaaa ccgaaagccc taatcgattt
    24061 tgcataattg aaggcagcca attcattaac ccaaaatagc cggcaatttg gacagttacc
    24121 atgatactaa ggccacatgt ttgccaagca agatgttttg attcaaacag tctgctaagt
    24181 ttggttttct tttcggcaga ttgtacagat ttgaattcct ttggttcagt caagccgcga
    24241 cgaatgaaca agaccatcaa caccggcagt acacctacga catacaacat cttccaacca
    24301 aacgttggaa tgatcacagc agctaaaatc gcagccaaag ccgcaccgac ctgcgcacca
    24361 ataccagcaa ccgaagaagc tcgtccaatt tgtttttttg aaaaactttc ggaaatcaag
    24421 gacatacacg ccccgtattc tcctcccgct cctacacctg cgataaagcg aaagagataa
    24481 accaagtaga tgtttgaagc aaaatacatc aataaagaag caatcgaaaa cagaaggacc
    24541 gtttgagaaa atactttcac acgtccatat ttatcagcta tgatgccaaa aaaaatacct
    24601 ccaatgagca tccccaaatt agtgatcgtt gaaatgaaac ccgcttgcgt accactaata
    24661 tgaaaagaag taatcattga agataatgaa aaagctaaaa acataatatc catattctct
    24721 aaagcaattc ccgccgatgt cgataataat aactttcgct gatacgttgt catttttata
    24781 ccccttttcc taaaatttcc ctaaattgtt aaaagtatat ccaaaaaaga ataaaaaaac
    24841 aagacttttt tatttgaaat aatacacaaa caaccgatat agataatcca gcttagtttt
    24901 aatagaaatg agtattgact tggagtacac ttaaagttgt agaataaata aaatacataa
    24961 gtaaagtggc aagaaattaa gagaaagagt gatcagatga atatcaaaca agtcagtgaa
    25021 gaaaagggca tttcggccga tacattgcgc tattatgaac gaatcgggtt gattccacca
    25081 gttaatcgga caaaaggcgg aatccgtgat tatacagaag aagacctaaa atgggtcgac
    25141 tttacgatct gtatgcgaaa tgccggatta tcgatcgagt ctttgattga atatattcgc
    25201 ctatctagcg aaggagaagc aactgtattt gagcggcgta agcttttgat tgaagaaagt
    25261 gaacttttga cgaaaaaaat tgctgaaatg caagaatgtt tggaacgttt acaaggaaaa
    25321 atcaagaaat atgatcgtgt gttatctaga aataaaatgg aatgtatcaa gtaaaaaaat
    25381 caaggagcat cgctatggag ttacgcttac taaaatattt ttggacaatt gcagaagaag
    25441 gaacaatctc tcaagccgca gaagttctac atcttacaca accaacctta agccgacaac
    25501 tgaaggagtt agaagaggaa ctaggaacag aactgtttgt acgcgaaaac cgcaaaatga
    25561 tcttgacaga agcaggttat tttctaaaaa agccgagcag aagaaatttt agatttaacg
    25621 aatcaaacca ctcaagaatt cgagaacaga aaaaaacaat tattcagcgg ccgatttacg
    25681 atcggctgtg tggaagctga taattcgaat acattggcac tattactaga aaagttgatc
    25741 cgtgattatc ccagcgttca atttcatatc cacagcggaa caagtgatga tatcacggag
    25801 tgactagaaa aaagcctgtt agatttagcg attttgctag atccagtaga tacagagaaa
    25861 tacgaaacga ttttgttacc aagaaaggaa aaatggggat tgctcatttt agaaaattct
    25921 gttttagcaa aaaaagacgt gataacaaaa gaagactttg cgaatgtacc tcttttgatc
    25981 tcaaagcgtc cagaaataca gcgaatgttt gctgactggt taggcataaa aaaggagcag
    26041 ttgctgatca caggaacgta caatttgatt ttcaatgtat ttgcgctaat tgagaatcag
    26101 gtaggtgctg ctttagtcat agaaggtgct gtaagtcatc tttcaaagcg agtgacattc
    26161 gttccgcttg acccaatgat tgaaacgaac tgcatgctag tctggaaaaa gaatcggatt
    26221 ttatcacctg tagtcaatga attgattcac cgtttcaaag aagcttttga acagtaataa
    26281 gtatctctaa tcgaagaaat ttcaatacaa aagcgagacg tatgacagtc ttgcttttgt
    26341 attttttcat ttttaaatca aaaaaaataa agagatttac ataacttgtt aaataagcgc
    26401 ttacaaacat aagaagaacg gaaatgaatc agctataata accgttgttc tggattgtaa
    26461 tcattacaaa gaaatgaaga ttataacttt ggaggtgttc gaaatgaaag aaaaaaagca
    26521 aaaattcgtt caaaaaacat gccctttatt caatggttgg gtatgattct gctgattgta
    26581 ttagctttat tgacgatcat tcctttttta tggatggttt ctgcttcatt caaagcaaat
    26641 aatgaagtgt tcacgattcc gattcaatgg atccctaaaa cttggcattt agagaattat
    26701 ctggttattt ggtctcgtat tcctttagtg acatttttta agaatacagc ttttttaagc
    26761 gttgtgatca cgttgattca gttgtttact tctagttttg ctgcttacgg gttttctaaa
    26821 ctaaatttta aagggcgcga tattttattt ttagcatata tcggcacgat tgctgtccct
    26881 tggcagtctt atatgatccc gcagttcatt atgatgagaa aagtgggttt aacatatacg
    26941 ttgtggtcgt tggtgctatt acaagcgttc aatgcttttg gtgtgttctt gttgaagcaa
    27001 tactacagta gtatcccaga ttctttatgt gagagtgcgc ggatcgatgg cttatcagag
    27061 tggggaatct atcgcaagat cattttacct cttacaaaac cggcgattgc cagtttgacg
    27121 atcatcacat tcgttaatac atggaacgat tatatggggc cattcattta tttgtcttct
    27181 acggaaaata aaacaatcca gttagggttg aaaatgttta ttgggctatt tgatgcagag
    27241 tacgcattaa tcatggctgc ctcagttgtt tctgttcttc ctgttgcttt tatattttta
    27301 gcgatgcaaa aatattttgt agaagggatc gcttcatcag gaatgaagga ataggaggtg
    27361 attatgaaag agcagcgttt tgacaaaaat gtttatatgg aattctttta ttggttgtat
    27421 caatcattgg caagtagcct ttgtttttgg tgtgtcaata tcttgtatat agcagctgcg
    27481 ttccttttgt cgatagatgg aaggaatgtc ttttccttta ttttttcttt actttttatt
    27541 ggtccaggtt gcatcgtgat gataggtatg acagaagaat tagcaaaaga aaaaagtttt
    27601 tcaccagtac gtttgttcat aaagacgttt cgccaatttt ggctaaaggg atttttttat
    27661 tggctatttg cttggatagt tagtgtgata atgatttttg attgtttctt ttttatacgt
    27721 ttttcttatg gaaaatggtt gattccgttg tttgttttac ttgcttgttt atcagtttcc
    27781 tttagcatca actactggta ttttcaggtg agaaatccag cgtcgaaacc aaatcaagtt
    27841 ctacggatag cgttctacta tacgttgaaa aaatggtatg tgagtttact agatttttta
    27901 ctattgacaa gtttgtttct cttctttttc gtaaagcccc aatggtgtat tctactaggt
    27961 ccttctatcg tatttggttt gatttacttc aacaatcgta aattgatgag aactatggat
    28021 ctgtagactt tataaattga aactttcaat taagaatgac agtttttatg taaaaactgt
    28081 cattcttttt cattttttga aacatatggc taaacaaatg aaataaaacg tgctatactt
    28141 ttgaatgaaa caggtttaga aactgtatca attatttgtg attatttcta actaataatt
    28201 aaaaataaag cataaaaagg acgatgttca atggacgaaa aaatgaaaca aggttcgaaa
    28261 tctgtagtag aaagtttgat taatcatcat gttgattatg tattcggtat accaggtgca
    28321 aaaatcgatg gtgtatttaa tgaattagag gatcaaggtc ctgaattaat cgtgactcgt
    28381 cacgaacaaa atgctgcatt tatggcacaa gcaatcggac gtattacagg agagccagga
    28441 gtagttatcg caacaagtgg tcctggtgct agtaacttag ctacaggttt ggtaacagcg
    28501 acagcagaag gtgacccagt tttagcaatt gccggacaag taaaacgaag cgatctattg
    28561 aagttgaccc atcaaagcat ggataatgca gcactgttcc agccaatcac aaaatacagt
    28621 gcagaaatcc aagatccaga aacaatttca gaagtgattg ccaatgctta tcgtatggct
    28681 aagagttcaa aaaaaggcgc gagctttatc agtatcccgc aagatgtagt ggatgcacct
    28741 gtccaaggga atgtcatcaa gccattgagc gatcctaaat taggttctgc ttctgcagat
    28801 gatatccgtt acctagctga acgcattaga gaagcaaaac taccggtact attagtaggt
    28861 atgcgaggat caagtgaaaa agaaacgtta gccatcaggc aattagttgg aaagacagca
    28921 ttgcctgttg tagagacatt ccaagcagct ggagtgatct cacgtgaatt agaagcgcat
    28981 ttctttggtc gtgtgggttt atttagaaac caaccaggag atatgctttt gaaacgaagt
    29041 gatctagtta ttgctattgg ctatgatcca atcgaatatg aagcaagaaa ctggaatgcg
    29101 gaaaaagatg cgcggatcat tgtgatcgat gaagcgccag ctgaaatcga tccttttatg
    29161 cagcctgaaa gagaattgat cggtgatatc tcagccactc ttgatttatt gacaggttca
    29221 ttagagcctc aacaagtgtc agaagatgca aaagaatatt tagcatctct tcaagcagaa
    29281 ttaacggagc gggacattgt tcaatcaaaa ggagaagcag gtatcttgca tcctttagaa
    29341 gttattaaca cgcttcagtc aaaagtaaca gatgatatga ctgttaccgt agatgtaggt
    29401 agtcattata tttggatggc acgccacttt agaagttatg aacctcgtca tttgttattc
    29461 agtaatggta tgcaaacgct tggtgttgca ttgccatggg cgatttctgc tgcattggtg
    29521 cgtccaaata cacaaatcgt atcagtttcc ggcgatgggg gatttttatt ctctgcccag
    29581 gatttagaaa cggcggtacg taaaaaattg aatatcgttc atttgatttg gaatgatgga
    29641 cactacaata tggtagagtt ccaagaaaaa atgaaatatc agcgggcgtc aggtgtagac
    29701 tttggtccag ttgattttgt gaaatatgca gaagcttttg gcgctaaagg aatccgagct
    29761 acaagtgtag aggaattaga aaaagcttta gaagaaggat ttgcgacaga aggacccgta
    29821 attattgata ttccgattga ttaccgtgat aatgagaaat tgggagaaac aatcttacca
    29881 gatcaattct attaaggaag tgtcgacatg actgaaaaaa tcttatacca gcacgggaca
    29941 ttaggagctt tgatggctgg cttgatggat ggaacagaaa cgattgctca tatattagaa
    30001 aaaggaactc tgggattggg aacattgcat gggcttgatg gtgaagtcat ctttttagat
    30061 ggcgtagctt atcaaggaag atcagatggc tcagttgccc aattagatgg aagtgaactt
    30121 accccctatg cagcaattac agattttacg ccagatacta gtttttctgt ctcaaaaact
    30181 gcagatggag aacaattgaa aaaagacata ttagtaagag aagccgggga aaacctattt
    30241 ttggcagtga agataactgg attattcaaa aatatgcaca tccgtatcat gccgaagcaa
    30301 gaaaaacctt acagaagact agcaaaaatt tcagagtctc aaccagaatt ccaacaaagc
    30361 aatgtccaag gaacattagt cgggttcttt acgcctgaat tattccaagg agttgctgca
    30421 gcgggatttc acttgcattt tatcgatgat acacatacat ttggcggaca tgtaatggat
    30481 tttgaagtgg ctgaaggaaa agttgagatt agccgcatct cgtcccttgt acagcatttt
    30541 ccagttgatg atccgacttt tgttcacgaa acgattgatt atgctgattt agcagcagaa
    30601 atccaacaag cagaatagaa aaagacagcg agatgcgttc aaggtgatta acctgacgta
    30661 tctcgttttt ttgaggagga aacctagcgt ttacaaaaaa actgagctat aatcttctcc
    30721 atagatcaaa aaggagcggt ttttatcatg tggacagaaa aacagaaaaa agatgcatac
    30781 gaaattttat tattacagca acaaggatta ttagaagcag aagataactg gttagcaaat
    30841 ttagcaaatt catctgcatt gttgaatgag actttgcctg agactgtctt tgcaggatat
    30901 tatttattcg atggtaatga attgatttta ggaccgttcc aagggcgagt ttcttgcaca
    30961 cgtatcaaga tgggaaaagg agtttgtgga gaatcagctg aaaaacaaga aacattgatc
    31021 gttgatgatg tcaaaaccca tgcaaattac atttcctgcg attcagccgc acaatcagaa
    31081 atcgtagtgc caatggtaaa agaaggtaat ttaatcggtg ttttagatat cgattgcgga
    31141 gtaacgaaag ggtatgatga aatcgatcaa gcatttctag aaaaatatgt agatctttta
    31201 ttagcaaatt ttaatataaa tgctttttca tttattaata actaaaatcg tctattgaaa
    31261 caaaaaagaa gagaaatcaa gaataaaaag agccggaaag acagtgggca tttctgtttt
    31321 tcggtttttt tattttttta taaaatcaca agtgttattg attttcataa tcatatttta
    31381 atggtattat cattttaaat aatggaaaaa cattaaggat ggatgaaata tgaataacga
    31441 aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac aaaaagtggt
    31501 tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg aatatcatga
    31561 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
    31621 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
    31681 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
    31741 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
    31801 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
    31861 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
    31921 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
    31981 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
    32041 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
    32101 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
    32161 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
    32221 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
    32281 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
    32341 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
    32401 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
    32461 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
    32521 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tag
//
LOCUS       DAPBAB010000027        31189 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPBAB010000027 DAPBAB010000000
VERSION     DAPBAB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.027"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(244..2316)
                     /locus_tag="RD444_001530"
     CDS             complement(244..2316)
                     /locus_tag="RD444_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7548910.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2249..2626)
                     /locus_tag="RD444_001531"
     CDS             complement(2249..2626)
                     /locus_tag="RD444_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDT7548911.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2755..3333)
                     /locus_tag="RD444_001532"
     CDS             complement(2755..3333)
                     /locus_tag="RD444_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7548912.1"
                     /translation="MRGNMKMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYL
                     SLRFLGYFTKESTIDPEDSYFWMINDFIATLP"
     gene            3465..4757
                     /locus_tag="RD444_001533"
     CDS             3465..4757
                     /locus_tag="RD444_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7548913.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATDNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            complement(4817..5935)
                     /locus_tag="RD444_001534"
     CDS             complement(4817..5935)
                     /locus_tag="RD444_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDT7548914.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5949..7370)
                     /gene="arcD"
                     /locus_tag="RD444_001535"
     CDS             complement(5949..7370)
                     /gene="arcD"
                     /locus_tag="RD444_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDT7548915.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7617..8738)
                     /gene="mnmA"
                     /locus_tag="RD444_001536"
     CDS             complement(7617..8738)
                     /gene="mnmA"
                     /locus_tag="RD444_001536"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDT7548916.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            9142..9741
                     /locus_tag="RD444_001537"
     CDS             9142..9741
                     /locus_tag="RD444_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7548917.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9891..11164)
                     /gene="efmA"
                     /locus_tag="RD444_001538"
                     /pseudo
     CDS             complement(9891..11164)
                     /gene="efmA"
                     /locus_tag="RD444_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(11435..11779)
                     /locus_tag="RD444_001539"
     CDS             complement(11435..11779)
                     /locus_tag="RD444_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDT7548918.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11916..13061)
                     /locus_tag="RD444_001540"
     CDS             complement(11916..13061)
                     /locus_tag="RD444_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7548919.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            complement(13241..14215)
                     /locus_tag="RD444_001541"
     CDS             complement(13241..14215)
                     /locus_tag="RD444_001541"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDT7548920.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLTIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14295..15170)
                     /locus_tag="RD444_001542"
     CDS             complement(14295..15170)
                     /locus_tag="RD444_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDT7548921.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELTNCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15300..17207
                     /locus_tag="RD444_001543"
     CDS             15300..17207
                     /locus_tag="RD444_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDT7548922.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAIYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17748..21014)
                     /locus_tag="RD444_001544"
     CDS             complement(17748..21014)
                     /locus_tag="RD444_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDT7548923.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEGNAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPIRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNQATGEVLENAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEQYVEM
                     ASYETKEILHTVDYRKPNYSTVYVTPEGSDTGNGSRQNPLSLTQALRYAYAGQTIVLA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWVLR
                     GFDVTNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTVKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIDVVVYVMKQRKK
                     "
     gene            complement(21285..21830)
                     /locus_tag="RD444_001545"
     CDS             complement(21285..21830)
                     /locus_tag="RD444_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7548924.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21842..22534)
                     /locus_tag="RD444_001546"
     CDS             complement(21842..22534)
                     /locus_tag="RD444_001546"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDT7548925.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSQDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22553..22837)
                     /locus_tag="RD444_001547"
     CDS             complement(22553..22837)
                     /locus_tag="RD444_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548926.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22850..23413)
                     /locus_tag="RD444_001548"
     CDS             complement(22850..23413)
                     /locus_tag="RD444_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7548927.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23640..24290
                     /locus_tag="RD444_001549"
     CDS             23640..24290
                     /locus_tag="RD444_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDT7548928.1"
                     /translation="MIKRNWPWLLALVLLFLFGFSFVLGLLLIGVVLVLWGLFGSRRK
                     KGKQEEIPALTNELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAKLKAI
                     DLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIKNKQT
                     YEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQKD"
     gene            24414..25610
                     /locus_tag="RD444_001550"
     CDS             24414..25610
                     /locus_tag="RD444_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDT7548929.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25711..27087)
                     /locus_tag="RD444_001551"
     CDS             complement(25711..27087)
                     /locus_tag="RD444_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7548930.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSFSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIISILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(27124..27990)
                     /locus_tag="RD444_001552"
     CDS             complement(27124..27990)
                     /locus_tag="RD444_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDT7548931.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(28079..29104)
                     /locus_tag="RD444_001553"
                     /pseudo
     CDS             complement(28079..29104)
                     /locus_tag="RD444_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(29122..29796)
                     /locus_tag="RD444_001554"
     CDS             complement(29122..29796)
                     /locus_tag="RD444_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7548932.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29977..30873)
                     /locus_tag="RD444_001555"
     CDS             complement(29977..30873)
                     /locus_tag="RD444_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7548933.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(30947..31072)
                     /locus_tag="RD444_001556"
     CDS             complement(30947..31072)
                     /locus_tag="RD444_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7548934.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         9030 a         6663 c         5571 g         9925 t
ORIGIN      
        1 aacggttgtg taaaaatatc attttagagc atgaaaaaag gagtggattt ctccactcca
       61 aaaaagtcta acttttgggg gtcactacat tgtcacagtc cttttttccg aataaatggt
      121 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaagcaatt
      181 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct atcacaacct ctttcatatc
      241 ttattacttc ttcattgctg cttttagtgc tgctgctaat gggctttctt ctggttcttc
      301 tgcttgtgca tattttttca gcaagcgtcg ttcttcatgt ttggtcattt tcttcttctg
      361 ctctttttta tcaagcattt tttctgtcgt tccatcgtat ttacaacgga aataagcacc
      421 gtttttacct tcgatgattt ccattttccg gtgacattgc gggcatcgat gattagagac
      481 ttttggatct tttcttctgc ggtagttgca atctgaattg gtgcagacat agattttgcc
      541 gtcacgtgtg tttttctcgc gcagattggc cccacattct gggcatttct tttgggtaat
      601 cgagaaatct tgatattctt gtttgcttga tttgatctca ttcaccaagc gttttgtttc
      661 tttttcgatt ccattcaaga aggttcctgc ttgtttcttt cctgcagcaa tagcttcaag
      721 gtctttttcc cagctttctg tcaaatctgg tgttactaaa gagggattta ccaatactaa
      781 taattgctta cctttaggag atacttgcag tccttgaggg gtacgttcca tcagttcaga
      841 tttgattagt ttctcgataa tctctgcacg cgtagctggg gttcccagac tgtatttttc
      901 catttgacca agcaagcttg cttctgacaa tggtttaggc ggtgcagata gttctttttt
      961 gatggtgaaa tcgggattga ttctcatttc tttttcccat ttgactgttt ccgtctcagt
     1021 ttcagtttcg cctttccatc ctgcttggac gacacgattt tgccgaaaaa tgaatcgttc
     1081 tttaccaaat tcaaccgtaa cttttgtttg gctgacttta tggggttgag caaacagtcc
     1141 taaaaaacga gagacaatca tttgatagat tttttgctct tcattactta atttttcata
     1201 acgtgctctg ttttcagttg gcaaaagtgc atggtgatcg gtcactttac tgtcttggaa
     1261 tacttttgtc tgttggacga tggctccgtt tttcaaatac tgcttgacct caggagcaaa
     1321 gtcgctgact gcttgcaatc gttctttcat cgtacttttc atgtcatttg tcagatattt
     1381 gctgtctgta cgcggataag taacgatctt atgggtctca tacaaacttt gtacaagact
     1441 taatgttttc tttgcagaat agccatagcg ttgattggct tctctttgga tctctgttaa
     1501 atcataaggc aatggcgcag attccgtttt taatttttct tggacttctg tgacagttcc
     1561 tttgcttgta ctgatcaatt gaacaagttt ttgtgcttct tcttgtgttt ttaaagcaaa
     1621 ttgatttttt tgaagcattt tggcttgttc ttttccagct tgcaacgtga tcgtgaaata
     1681 cgtttgtgga cggaactgct cgattttctt ttcttggtct ctcactaatg ctagtgtcgg
     1741 tgtctgcaca cggcctgctg ataaattatc cgcgtacttc acagttaagg ctctggtcac
     1801 attcaatcct accagccagt ccgcttttgc ccgggcaaga gcagattcat acaatgcatc
     1861 atattctttc gcaggtctta gttgcttgaa tccagttttg attgctttat ctgtttgaga
     1921 agagatccat aaccgcttca ctggtttatt gaaatgcaca tactctaaga tccagcgagc
     1981 aacaagttcc ccttctcttc cggcatctgt cgcaatgact gcttctgaga catcttttcg
     2041 ctgagccagc tgcttgattg cttttaattg atgtcctgtt ttaggaagtg gtttgattcc
     2101 tagtttttta gggatcattg gcaacgtgtc catttgccag ctctgccatt ccttattcag
     2161 atcttccggc attttcaatc ctaataaatg accaagggcc caagtaacga tcaccttggg
     2221 tccttcataa taatttttgt gcttttgatt agcaccaagc accttgctta ggtctttcgc
     2281 aacgcttggt ttttccgcta gaattagttg tttcataaaa ttcctttccg ctttctactg
     2341 tgatgggctg atgatttttc aacaacgcaa gtcctttgta ttgctgtaaa tcatcgtcac
     2401 actcttcaca ccagcgctca tctccgtcat tccaaaaggc gctgagaaag ttactttttg
     2461 cattgatata ttcataattt tgatggattt cgttccattg tttttcgtac gcttgtcgtg
     2521 cttcttcgaa agactcgaat gtttcttctg cttcaatatc ttcttcccag tcttcaaaaa
     2581 accaccaagg ctcattgtcc ccgaacatgg taattacttg atacatactc gtgctccttc
     2641 tttcagcttt tctagactta tcttagtcac ttttcaagaa agttgcaaga attttctctt
     2701 ataaaaggat catttaatga acatcttgaa aatgatataa atgaatctga ttgtttaagg
     2761 caaagtagcg ataaagtcat tgatcatcca aaaatagctg tcctctggat cgattgtcga
     2821 ttcttttgta aagtatccta aaaaacgcaa ggaaagatag ccgtggatca tactacataa
     2881 ttctctgctt cggtggactt tttcttcttt cgttaattga aaagcctcaa agatctgcaa
     2941 aatgatctgg ttcgtttcat ggattccctc taataaaact gcttcattgg tgtggggcac
     3001 actgatcagt aactcataca ctgcttgatt tgaaaaagca aaggatcgat aagtttctgc
     3061 atagatctgc agtgcttcct gaccactttt tcccacaaga gcccgacgca gctggtcatt
     3121 aagttcctgc agaagtttcg ctgttagcgc tgttttgaca tcacgaatat ttttgaaatg
     3181 gttatacagc gagggagcct tgatatccaa gcgttttgct aaatgctgaa aggttatttg
     3241 ctgaacatcc atctcttctg ctagttctcg aaagcagtcg atgattttct cttgagacag
     3301 atttcttttt ttcattttca tgtttcctct cataaaataa atccttcact ctttcagttt
     3361 accacgaaaa agaaagagaa aaaagagact ttcattgata aactacatat tgtagtttat
     3421 aacaaaaaga tgtagatgaa aattttcaga aagaaggagt ctaattggct gcaagaataa
     3481 aggaaaaatt atttaggaaa agagaaactt gggaacaaaa cttgtttgtc ttatggtttg
     3541 gaacatttat ggctggtatc ggatttagtt tagtcatgcc ttttatgtca ctttacatag
     3601 atacgttagg taattatagt acgtcacaat tgaacttttg gagcggattg actttttcat
     3661 ctactttctt ggtgactacg ctgatctctc cttggtgggg aaaattagcc caccaaaaag
     3721 gaagaaaatt gatgctgttg cgtgcttcat taggaatggc aatcgtcatt tctttgatgg
     3781 gttgcgtgac tagtgtgtat caattagtcg cattgcgtct gcttcaaggg attttttctg
     3841 gttatatcag taatgccgct gcccttgtcg caactgggac gccaaaagaa aaaagtggtc
     3901 aagttcttgg aaccttagca actggttctg ttacaggaac attacttgga ccgcttcttg
     3961 gcgggatctc tgcttcagcg tttggctatc gccctacctt tttcattaca gggatcatct
     4021 tatttttcgt cttcttgttg agtctgtttt tcgtccatga aaaatttgtc ccagtgaaaa
     4081 aagaaaacat ggtttctgcc aaacagattt tcaaagactt gaaatatcca cacgtcgtca
     4141 tcggtatgtt cgtcacaacg atgatcatcc aagcatccaa caattccatt agtccaatca
     4201 tcagtctgta cattcgccaa ttgctgcatg gacatgggaa tgttacgttg atcagcggaa
     4261 tcatcgcttc gatacctggg attgccactt tgatagccgc tccaagattt ggtcgcttag
     4321 gtgacaagat tgggagtgaa agaatacttg cgattggttt gggttttgcg attctggttt
     4381 atatcccaat ggcattcgtc acgaatgttt ggcaattagc tgccttacgt tttttgatgg
     4441 gtatctctga tgcttgtctg ctccctgctg tccaagcatt gatcacaaaa tattctcctc
     4501 atcaagctgc tggacggatc ttcagttata accaatcttt ccaagcaact gataacgtag
     4561 tagggcctat gatcggctcc agtgtttcta gtgcatttgg ttatcgtggc gtctttcttt
     4621 cgacttctct tcttgttttg gtcaacttct tgcttgtacg acacaataca caagagatcc
     4681 atcagcaaga aactgcagaa gaaaaagctt caggagtctc catatcttca gggcactctc
     4741 cttctcatag acaataagcg taaatgaaaa acactgcttg aacagttgtc acagactgtc
     4801 cttgcagtgt ttttgtttag gcctgttttt tcaagaactc agataagagg aataatccct
     4861 tttccaaggt ttctttttta caacagtaac ctagtcgggc atgcttcggt aaatcaaagg
     4921 cacttcctgg tactaatagt actcctgttt cttctaataa attgatacaa aatgtctggt
     4981 catcttcttc gatggctaat ttaatgaaag aagtagagac gtaattggga gcgacaagtt
     5041 caactttcgg ttcattggct acccattgtt ttaaaatcac aaggttctct gtaatgatct
     5101 ttttgtttcg ctccaatatt ttttctttat tcttcaatgc atgtacagca agatcatctg
     5161 acaggactcc gccgcaaatc atcgtgtagt ctcgatactt tcgaaaaact tctgccaact
     5221 caggtccagt agctgtccag ccaatacgaa tacctggaat ggagtaggtt ttggataggg
     5281 aattggttgc tatacctttt tcatagacat ctacgatcga caagaattca cctttatccg
     5341 ttaaaggcgc atatacttca tctatcaata catatgcatc tactgtacga gcaatttctg
     5401 cgattttttc taaagttttc cgatctagca atgtgcctgt aggattgttt gcactattca
     5461 aacagatgat ttttgtttca ggtttgacta aggcttctag ctgttctaaa tcaaactgcc
     5521 aatcttcttc ttccttcaaa tgaacaaagt caacagcggc acctaaagat ttggggatgt
     5581 catataattg ctgataagta ggaagcatcg aaacaacatg gtctcctgct tccactaacg
     5641 cgtatagagc taaaagatta gcaccagtcg ctccattcgt ttgtaagatg ttttccggat
     5701 cgacggtttg ataaagagaa gcgacttctt gtttgaaaac atctgatcct tcgatccagc
     5761 catagtccaa tggttcgttt ttgcgttttt cgaaaaattc acttacgcta gtcccatcta
     5821 agccaacgag ttcgtctaat gtcaatgctt cgatcgagct ttgtgcaata tcatataccg
     5881 ctttcttttc ccatttattt agccattctt ctacaccgaa atttgcgatt ttcatgtttc
     5941 tttccccctt atatcttgat ccagcctaca gcaagcaacc cgattgaaag cagtgcggcc
     6001 acacaaatca gcccaatcat tactttttct tttcccgtaa tttgatgttt ggtttcacga
     6061 caagcgatcc agtagaagca aaaaccagga agataggcaa tactacataa aaggacataa
     6121 gaaaatcctg ctaaaatgat gcccatcaat tcaaaagcta cagcaatccc accgatcact
     6181 acttgttttt tgattccttc ttttaaagaa aactggactt gatagatccc gacaagtaga
     6241 taacaaatca aaattgccgc tgtacataaa gaataggcaa attgataggc ataatcagta
     6301 actaagaaag tcaataagaa tagattagtc aagattgcag taacaactaa cgaataagtt
     6361 ggggcatttt ttttattcgt ttttccccaa cctttcggca acagaccgtc tcgagccatc
     6421 aacatcgttg tttctgctgg gagcatcgtc caagacaacc aagaaccaat gatcgaaata
     6481 atcaagccga tattgatcag cgctgctccc caatttccca cgattccttt taaaatattt
     6541 gccatagcag gttgtgaaat agttgctaac tcttcttgag ataagacgcc gtatggcaag
     6601 atcgaagcaa ggatatagat aaacaataaa caaagcagac caatgatcga tgcttgctgg
     6661 gcttctgaac gtttcttcgc gcggttggct aaaacacttg cgccttcgat ccctacaaag
     6721 acccacatca taaccatcaa acaaccttta atctgcaacc agatcgttcc ttgctgccct
     6781 gttccattca ctagattatc agatacgtta ccccaaaaat cagcagtgaa tatattcact
     6841 ttaaatgcga taaatacaaa tattaaaaat aggaataatg gcactaattt acagatcgtc
     6901 acgattgtat tgatgacgct cgctccttca actccattgt ttactaataa agtcagaccc
     6961 caaataaaaa tacttgcaaa taaaatagaa ggaacatttt gtccaccttt gaatatcgcg
     7021 aaaaattctc ccatggcgct catcagcatc gttgcaaaag caacatttcc cagccacgca
     7081 gacaaccagt aaccccaacc actgatgaag ccacctaatt taccaaatga agcttccgcg
     7141 taaccgaaaa taccaccatc tagatctgga cgtttttctc ctaaatgatt cagcgataat
     7201 accaaagcta aaataccaat cccaacgatt atccatgaaa ggatcgctgg ccctggagct
     7261 gcagaactag ccagatcact tgtgatccca aacacaccgc ttccgattga cgaactgaca
     7321 accagagcaa ccagtcccca tttgctaata cccgttgatt tcttttccat ttccatcacc
     7381 ctcacttata aaattgaaca aatcacttgc aaaagatttc cttctatttt gcaactgctc
     7441 ttgcttaaca aaaagcaact ttattcatat tatcggatat tatgaacttt ttacacaagt
     7501 atttcaagta taaaatgaat aaaaattaat tttattgcaa aaaaaacagc tatgctatcc
     7561 aaaagaaaag gtgtttcttt tgttagccat agctgttttg attcatttaa tatcgcttaa
     7621 acgtattgta aaacttttgt ttcttgatat gcgtggtcga tcaaaccacc acccagacat
     7681 tccatttcat cataaaatac aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg
     7741 aatacgactt ctgcatgatt atcatccaat aagcgaactg tcacaggaac gtcttgctga
     7801 cgataacgga atttcgctgt acacttgaat tcttttggca ttggttcatt cgtcgtaaag
     7861 tggatttcac ttgcatccaa gcttgtggca tacaatgcag gatgatggaa cccttgcccc
     7921 acatataatg tgtttgtcgc tagatctttt cctacaacaa accagggttc ttgcgaatct
     7981 cctccgccac cgatccctag tccttgacgt tgaccaatcg tataatacat caagccagca
     8041 tgttgccctt tgacttcacc atcaagagtg accatatttc ctttttttgc tggcagatag
     8101 ttgctcaaaa attgtttgaa gttcttttca ccaataaaac agattcccgt tgaatctttt
     8161 ttcttagcag tcgcaaggcc tgcgcgttca gcaattgccc gtacttctga tttttccatg
     8221 ccgcctagag ggaacatcgt ttttgaaagc tgttcttgtg ataattggct aaggaagtaa
     8281 gtttgatctt tattattgtc tactccacga agcatgtgga ccacgccgtt ttcatcgcga
     8341 gtgacttgcg cataatgtcc agtagctaca taatcggcgc caagatccat tgcataatcc
     8401 aagaatgctt tgaatttgat ttctttgttg cacatcacgt ctgggttagg tgtacgtcct
     8461 gcgcggtatt cggctaggaa atattcaaat acacgatccc aatattcttt ttcaaaattc
     8521 acagaataat aaggaatccc gatttgagca gctactttcg ctacatcctt ataatcttcc
     8581 gtggccgtgc atacaccgtt ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc
     8641 acgtcgtatc cttgttcctt taacaaaagt gctgttacag atgagtcgac accgccgctc
     8701 atccctacga caacgcgtgt tttgctgttg tctgtcatga tatcaccatc atttcaatta
     8761 gattctgaaa catctacgag ttgaaggtcg caacttttca gtattttaca ttatacgtca
     8821 tatttctgaa aatttcatcc cttaattatc aagtcgtaaa aatactgttc tactggacct
     8881 attcagtaca caaacatgat agtcaaccaa aaaatcactc atcttttgag cattcgatat
     8941 aaaaaattgg catactcata aaaagggcca gcctttcggc aataaggaat aattttataa
     9001 attgtgaaaa actatttttc aaaattgctt cttatcgctt aaagctacac gtctcattta
     9061 caaccttttc tttttagatg ttatgagttg ctcagataag tgattttgtt tactctaaga
     9121 aaaaagggag gcaaaagata catgaattac tttaccagtg atatgcactt tttccatgaa
     9181 cgcttattag gaaaaaacga ctttgctccg cggctctttg caaatgtgga tgaaatgaac
     9241 caacagttga tcactagctg gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta
     9301 ggcgaccttg ctatgcatcc gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc
     9361 actcaactgc aaggaacaat ccacttgatc aaagggaacc atgacagtcg cgcttttttc
     9421 cgttatttag aaaaaaatga tcctaggctt actgacggaa gtcccaaatt ttatttttat
     9481 gatgtaggtg ccatcgttaa attcgaccat caacaatatt acttgactca ttatccgatg
     9541 ttgctcgggc ctaacgacac tatccgcaat cttcatggac atatccatca ttacagcgta
     9601 cctatcgaga acgatgtcaa tgtaggcgta gacgcaccgg aaagggaact cttaaaggaa
     9661 aaactaccat ttggcactcc gttatctgag caacaaatca aagaaatcta tgaagcaaag
     9721 aggaagtacg ataggtcgtg aaaaaactgc cctatatcaa gtattaaaca aataaagata
     9781 attactcata tttttcattt gagtaggctt attgccacag gaacattagg atcagaaatg
     9841 gagttgtaac aagacttctg tcacaactcc attccatact attcgtcttt ttactcatgg
     9901 tgttctttta ccaatttttt ctgtaattct ttatcataat ttcgagcagc tgacgttaaa
     9961 aataaaacga ttccgcataa taaaatcccg attccagaaa acaagaacat tttttctacc
    10021 cctaaacgat cagctaatgg tcctgcaaat aataatccaa ttggacctgc caggttcatc
    10081 aaagagttga agttccctaa gacacgacct aaaacattcg attcataact ctgctggatc
    10141 attgccatca gcagtgtatt gaaatatggc gttgcacaac ctgctcctgc attcaagata
    10201 agaaagtaca agaacccttg actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg
    10261 cccataatga tgtaggctat aataataggt ttcattcggt ctttccatgt tcctataaaa
    10321 ctgatcagtg caccgcctaa gagcattccg actgcgtaga ctacttccac tgctcccgcc
    10381 tgtccaacgg aaccaccaaa gtaatctagc gtcatcaatg gatacagact gattgctggc
    10441 atgaacaaca gcatcacaaa tgcaccattg atcgttatgt accataaccc tttgttatcc
    10501 atcaattgct gcagcccaaa ttttgcatct ttaaaaatag taagcggaac ttccaatatt
    10561 tcaggagaaa cttttgggat cttcacaaaa atcaataagc cgacaccaaa aactgccccc
    10621 aatacatcta ataaaataag gtaattgaca ggaacgactg aaaacaaagc cgcacctaga
    10681 gctggcgcaa tgatgaaatt cgctgattgg accattccta actgtccatt cgtccttgtt
    10741 atgtgagaag atggaacgat tgtcggcata atcgactgat cgttggcatt tggaatgttt
    10801 gtgcgaccga acgcataaac aaagacacaa aaactagcca aatcgggaag gattctgaaa
    10861 tcgttccgac tactgctaaa ataagcgcga aaatcgctac aataatatcc gtgacaatca
    10921 aaagagcttt tttatcccag cgatccacta gcggttctac aaatgggctt agtaaaacca
    10981 tgggaatcat tcctagtaaa gtagcaaaac tcaaaattgt cgccgaaccg gtttctcttg
    11041 tcaaatacca gatgatcgca tattggacga ccatactagt gattcctgac aaaaactgtc
    11101 ccgataaaaa gaacaaataa tttctcttcc aattcgttaa aacgaccgac tgttcatttt
    11161 ccataaacat ccctcttttc tttttcattt tgtatagcgt ttgcacaaca atcattatac
    11221 gggattcttt tttcaaaaaa tacctttttc cctaaataac aatcgtttca atttaaattt
    11281 ctttacttag aaacaataaa tcagcaaaat attaaaaata acaacatgtg ttaaatttaa
    11341 aaataaaaaa gacaactaat ttgcgtcgtc cttacaaagt tagtcatcta acaataggcc
    11401 gtttcaaaac tagttgtctt tttatatcca cagatcattc gactttttcg aataacccac
    11461 ggctaatcat cccatccatc agaaaataaa atttatcttg gctcattttc tgttttggat
    11521 ctttgaaaat attgactgct ttcaatccat ttgctgtagt gatcgacatt ttcaatgttt
    11581 gaatatcttt tgatacagtg atttttaatt tgaatccttc tttgctttgc ggcgtggcat
    11641 ctaatggtcg gatcaattga taattgcctc cgtttgtttc tgtaaatcca ttatcgcgca
    11701 atgtatatct ttttgcatca ggatgcaaac gatagctaat tggtgcgccc agtaccgttg
    11761 ctgttgtttc aaatgccatg attttcacct cgataaaatt agtttctctt tattataaaa
    11821 gtttatcctg atttttacta ggtttttcag ttaagttccc taaaaaatga aggcgttctc
    11881 tcagacatct gttcatataa gaatgacttc actagttatt ttttgacagc acgaactaat
    11941 tgttcaagga aatactggat gtcgtcttcg gtatttccca aaccaaagct gatacggatg
    12001 gattctttta gtatcggtgt atcttctcca tacattgctt ctaatacgtg tgaaggctcg
    12061 atattacctg ctgtacaagc tgaaccagta gaaatagcga ccccattaag atcaagcttc
    12121 atcagtagca aatcatttgc ttcgccaatt aagcgaagat tcaacacatg agggagttta
    12181 ttcgttaaat caccatttac ttgataaggg atttcgtttg cttctaatgt atcaagaatc
    12241 aattgctgga atcctttata tttgttgtta cgtgcttcac gttcttcagg tgacagcagt
    12301 tcaactgctt ttgccatacc ccagatccct gctaaatttt ctgtacctgc acgccgcttt
    12361 tcttcctgct ccccgcctaa aagcaatgca gggatattta ctccgtctcg tttatataag
    12421 aaaccgacgc cttttggccc attgatttta tgagcagaga tacttaataa atcgattcct
    12481 aattcttctg gatggatcgt ttggtttccg tacgcttgta ccgcatctgt atggaaataa
    12541 gctggatgat ccgctaaaag ttcaccgatt tctttgattg gcatcaaatt tccgatctca
    12601 ttgttcccat acatcacaga gaccaagatc gtatcttctc gtaacgcttt tttgacctct
    12661 tcgatcgaca gattcccttt ttcatctaca ggcagatatg taacgtcaaa cccctttgtc
    12721 tctaaatatt tcatggtatt aagaacagca ggatgttcga tagctgtagt gattagatgt
    12781 ctgccttctt tttgacacga gaacgctgtt tctaagatag ctgtattatc cccttctgtt
    12841 cccccactgt taaagatgat ctcgtgaggt tttgcttgca gactgtctgc aatgatttgt
    12901 cttgctgttt ccaatttttc atgcgcgtgg cgaccaaatc catgtatgct tgaaggattt
    12961 ccaaacgtgt cttgcatgat tgccgtcatc gcttcaatta cttgtggatg catgggggta
    13021 gttgccgcat gatccagata aatagttttc acttcattca tcccttccaa attattgcta
    13081 ggcacattct agcataaaat agcgaacaaa ccaaagaaga gctcattttt gtctagagga
    13141 cgtttcatgg gcatagaaaa aaggagctat gacaaaagtc ttgtcgcagc tccttgaagc
    13201 tgctcacttg tgtcacaact tctttccctc ttttttttgt ttattcgaat tctttttctt
    13261 ccagacggaa taacggactc atcggtgtat tttcatggat gcgtttgacc gcttctccca
    13321 ttaattctga acatgtaacg attgttaagt tttctggatg acgattttct tctgtgaaaa
    13381 cagaatcagt gatacaaata tctttgattg gtgcattgtc taaaatactt ttcgcatgat
    13441 cggacaataa tccatgtgac gcacaggcat atacttcttt tgcgccgttt tcttttaata
    13501 catttgctgc tcgtgcaagc gttgctcctg tgttcaagat atcatctacc atgatacatg
    13561 tctttccttg aacatcaccg atcacataac caccctcagt tgaagcatca tctgcatgat
    13621 ctacgatggt taatgtgctg ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc
    13681 tgttttttgg cgaaacaact acgatgtcat ctcccgtcag acctaattga cggtaataat
    13741 gagcaaacaa cggcatcgta aataaattat ctacaggaat atcaaagaat ccttgtactt
    13801 gaaccgtgtg cagatctaaa gtcagtactc gagttgcccc tgcttcttcg atcaagttag
    13861 cgatcaattt cgcagtgatc ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc
    13921 catagtaagg caatacgaca ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca
    13981 tgatcagtag ttccatatag tagtcattga caggtgcatt cgttgattga acgatataca
    14041 catgatcccc tcgaatactt tcttcgatat tgatggaaat ctcgccatca ctaaattgct
    14101 tcacgacgct tttacctaat tctgttccga acactttcgc gattttttca gctaatggac
    14161 ggttgccatt caaactaaaa atcttcaacg tgtcatcctg atacttatta ctcatgtagt
    14221 cctccccaaa gcttctctat tcttcgatag tttaacatat ttttccaaga ttttctgtta
    14281 atttcagcaa attcttaagg aagtgaaact gttccgttac tccaactggc aggaatgact
    14341 ttatgataat cgccccagtt gtcgtgtact ttgtaataac gttctcctgt ttcagtttca
    14401 aaataagcgt acgctgtcac ccaatgattg ccatatgtac tgccaagtac tttcaaaata
    14461 ccaatcatca caggtttgcc ttcacgtata cgttttgtag ctcgctgcca tgaacctaga
    14521 agagttgctc gtgcacgctc atgattgcca ttcttcttta aaaaactaga aatccctgta
    14581 ctaacctgaa aaggaatcgt aggaagtcct agtggttgga tctggctacg caatgccttc
    14641 gtcaaaacca acgaatcagc cgtatttttc ttgcgaatct catttggaag catccacccc
    14701 ttacgatagt cttgataata ggcaaggaga actgctgccg cgtaagtacc acaaagatag
    14761 cctgaggggg tagcccattt cttatacctc aaaaaacgtt cttctggcaa tcccacccag
    14821 caatttgtga gttcctttcc tgaccttttt tcttcgatca aaacgggaat atcttctttt
    14881 ctgttgatcc atctagataa ctcgattggc tcttcattga tcagcttcca ttttttacaa
    14941 gaaagaccct cttgataagt atattttgct ggagaatatt ctaccggatg tcctgattga
    15001 tcgataatca cataactttg atcttcaaaa accagagcta aaaaaagcaa tttacttttt
    15061 cttgactgca gctgctgagt gctggctaat tttttaattt ttttatctac tttgaaatat
    15121 aaaggtttat ccactacttg attcagttgg gatcgcctcc tttttttcat tataagtgaa
    15181 ggattgaaaa aaacaaatca gtttgattgt tcgaattttc aattttttat gttctatttg
    15241 ttaattcctt cattttgtaa gataatgggc acacgaaaca aaaaaggagc gatcgatgaa
    15301 tgaacaaaga acgattgaaa caagatttct atgaagcagt caatgaagaa tggcaaaaaa
    15361 cagcaaaaat ccctgcagat aaaccagcta caggcggttt ccaagattta gttgacggga
    15421 tcgaccaact tttgatggga gaaacagacg atctgcttgc ccatccagaa aaagtgtctt
    15481 cagaaaaaat gaaacatttc cttgcatatt atcaactggc aaacgattat gaccgaagaa
    15541 atgagttgca tgctcaacca cttcaaccta taatcgaaaa agttgaatca ttgcagtctt
    15601 tcgacgaatt gaataagcaa tttccagaat ggctgttgga tacattacca ttgccattta
    15661 atctggatgt agatgcagat atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc
    15721 cttctttatt ccttccagat aagacgtatt atgcatcaga ccatcccaac ggtgcacaac
    15781 taataggcgt tttctttgat atggctgttc agttattaga gcttgccggc aaagaaaaaa
    15841 gtcaggcaga agcaatcacg gaacaagcag tgcaatttga taaattgatt gctcctcatg
    15901 taaaaagtgc agaagaaaac gcagattaca gcaaaatgta taatccgcaa tcttttgata
    15961 catttattca atacacagat aaactggact tgaaaaaact tgtcacgggt ttgattggga
    16021 caactccaga taaagtgatc gtaactgatc cagtgtactt cgatcatttg aatgacatcc
    16081 tgacagaagc tcattttccg ttattcaaaa gctggatgat cattaaaaca gtacgttctc
    16141 ttagtggtta tctatcagaa gaattccgcc aagtcagcgg aatctattct cgcgtattgt
    16201 ctggaacaga cgaagcaatg cctcagaaaa aagcagcgta ttaccttgcc tctggacaat
    16261 tcgatcaagt agttggcgat tactacggaa aaaaatattt tggcgaagct gctaaaaaag
    16321 acgtagaaca aatggtgaaa aaaatgatcg ccatttataa aaaacgtcta gaagaaaatg
    16381 actggcttgg ggatgcaaca aaagaaaaag ccgtcataaa attaaataac ttaggtatcc
    16441 aagtaggcta ccccgataag atcccagcat tattcgacca gttcaaaaca gtacctgcag
    16501 atcagggtgg tactttgctt tcaaatactc ttgttttcag caaactcaca ttaaaagatc
    16561 gcttcagcaa atggaacaaa ccagtggatc gttcagaatg ggaaatgagt gctgacacgg
    16621 tcaatgctta ttatcatcca tttcggaatg tgatcgtctt tccggcagcg atccttcaag
    16681 caccatttta cagtctagaa caatcaagct ctgctaatta tggtggaatc ggagctgtta
    16741 tcgctcatga gatttcccat gcatttgata ataacggcgc tttatttgac gaatatggca
    16801 acttgaacaa ctggtggaca gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa
    16861 tgatcgaaga attcgatggt ctgccttttg ctgacggaaa agtaaacgga aaattaaccg
    16921 tttcggaaaa tattgctgat gcaggcggtc taagctgtgc attggaagca gcaaaaacgg
    16981 aatcagatct ttctatcgag gaattctttg tcaattgggc aacgatttgg cgtacaaaag
    17041 caaaaaaaga ataccaacaa cttttactgc aaatcgatgt ccatgcccct gctaaactcc
    17101 gagcaaacgt gcaaccaaaa aaccttgacg aattctatga aacatttggt atcacggaac
    17161 aagacgctat gtatctagcg ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag
    17221 aagtggtcag cttcaagaaa taagaaggaa ttggggaaaa ttgcttctca aatttttgtg
    17281 acatttcggc ttattttttg aagaagttac ttctggaata ccatgtatca gaggttgtga
    17341 aaaagctgct tagtttcaag tagtaagaaa aattttgtga aaatggcttt ccacattttt
    17401 gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc gagaggttgt
    17461 gaaatcagcg tcctgacctg agtattagag aaaaaaacgg aaaaatagtt cctcatattt
    17521 tgccgtttgt tgagctaata ccgagggtca gctgattgaa caccgtttag tggagaggtt
    17581 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaagcag caaggagaac
    17641 ctcatctata aaaagataga aggaagtgag acatctattg gtctcatttc cttctatttt
    17701 tactttcctc agtcaattgt tttacatcat aaaaggagaa attccactta tttttttcgc
    17761 tgtttcatga cataaacaac tacatcgatt aacaaaattc ctccagctaa tgctagcaac
    17821 gggttattaa attctcccgt ttttgggagt ccttgagtac catttttttc actttctttt
    17881 gctgtcctat ccggatgaga aggttttcca tttggtgatt catttcctgg attttctctt
    17941 tctggtttgt catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc
    18001 tgggtaacag attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa
    18061 gtgacttcta gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa
    18121 aattgagcac ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct
    18181 tcaaagacag gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc
    18241 agttcaatga tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc
    18301 ggattatttt gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga
    18361 tattccgaag acggcgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca
    18421 tccactcttt cctttactgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca
    18481 tggatacttc cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt
    18541 agagactcga tccaatcttt tgatacggta tgtccaagag tattttctga ttgtttctct
    18601 cgttcattcc aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg
    18661 cggatctgct ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg
    18721 tagtctgtgt ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa
    18781 cttgtactgc tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga
    18841 ttttcgaaag acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt
    18901 ttgaatccat taccgttacc ttctccctca attcccggta cccagccgtt tcgataagca
    18961 actgagtttt tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc
    19021 caaccgtcat ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt
    19081 gtcagtttgg ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat
    19141 gtacagttct tgactaagtt atgcgctggc catgcttcga aaggatcatt tgagctacca
    19201 ctgatttgga ttcctgtatt tccattccga taagcatgga tctcttcaat caaattatgg
    19261 tttccagaaa tctgcaatcc tttttgcata gcggcactat ttgtaacgtc aaacccacgt
    19321 aaaacccagt aatgactaaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca
    19381 ggtcgcttgt tttctggatt tttctctgca atcaattgaa tcgggttttc tcttgtcccg
    19441 ttaactcctt ttggaatcgt caatcctctt tcaaaggaat aagttccagg agccaggacg
    19501 attgtctgcc cagcatatgc gtatctcaat gcttgcgtca gagataacgg attttgtctg
    19561 cttccgtttc ctgtatctga cccttccggt gttacataaa ctgtcgaata gttaggttta
    19621 cggtagtcaa ctgtatgcag aatctctttt gtttcataac tagccatctc tacgtattgt
    19681 tctggcggat agtttttatc tggtgtaaaa ctgaaagtaa attcgttcgt tccttccatc
    19741 agttttgtct gataatcaaa ttcccgattt gcagtgattg ccaagtcttt tgcgttctca
    19801 agtacctctc ctgttgcttg atttttgatt gttaataatc cgtcagcatt tgctctaaag
    19861 gacatagtat ggtcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca
    19921 atcaagcgaa tgggcctttc ttgtccagga ggatctgtcg caggatctga gtaagccact
    19981 tcgattcctt ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc
    20041 gattcatctg aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct
    20101 cctgtttcag gatcgtaata atatgcctct aaaccagtat tcgttttttt catctctacg
    20161 tccagtttag taatcgagtt cgtggccgga aacagattcc catgaccgtt tccgaatata
    20221 ttataagaac ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc
    20281 actgagacac tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt
    20341 gcccgtgtcc ctaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct
    20401 tgtctatccg agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa
    20461 taaaatgcct gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat
    20521 ccttcttggg cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag
    20581 ttgatgtctt tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg
    20641 aattttccgc cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca
    20701 tacctactag cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct
    20761 tcagattgct tttttgtctt ttcattttct gattctttca tcataggatc atttccttct
    20821 tccgtatcca tatcttcact agttcttggt acttcctcca ttgttgacat cccttcaatg
    20881 ttcacgtgtg catttccttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca
    20941 gaaattaaaa ccggggtact gagcataagg agattcatca ttagcgcact gtattttata
    21001 cgttttttta acaagtgtat tcctcccgca acaaattaga aagcgtttac ttaaagtatt
    21061 acctaattta tttttactgt caataaaaaa caaaattata ataagtgtat tttataaaaa
    21121 gaaaaaatat aacttaagtt gttgataata aagagattta ttaacgtaaa aagaataaga
    21181 tttttcaaaa taacgtataa aaaagagcat aaaacaaagt cgtttgtttt atgctcttct
    21241 tttattattt tgtgcttttt attttatcaa attactttgt ttttttataa aatttctgcg
    21301 cctagtgtgg attcaaaatg tctcaatgcc cattcatgtc caacgggatc gaaacttgcc
    21361 acggcatctt tgaaaatatg gagtttgtat cctagattat acgcatcgac tgctgtatgc
    21421 aacacacaga tatctgtaca tacgccggtc aaataaatat ctgtgatctg gcgttctcgc
    21481 aaacggatgt ctagatctgt tccactaaaa gcggaataat gccgtttgtc tatccaataa
    21541 acattttcct cttgctcata gtcttggtat aaacttttta aagagccgta caattcccgt
    21601 ccacccgttc ctatcacatt atgcggagga aataagcggt tctccggatg aaaggaatct
    21661 tttggatcat gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt
    21721 gtatgagcag tcagcttttt ttcaattact tgtcctgctg tcccagtagt cagctttcca
    21781 tcctctgcaa caaaatcata tgtgtagtca atcgagatca atgcgttcat cttagtcctc
    21841 cttatacgag atgtttgaca aattcgatca ccatttctgc tgatcgtttg cctgcgtctt
    21901 cgataaattc gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta
    21961 cgatcaaaaa tgggatattg aactgacgag ctgtttgtcc aacagcggct ccttccattt
    22021 cgcaagctaa tgcttcaggg aaattcgtta agatttcttt gattttatct tgcgagtcga
    22081 caaatgtatc acctgtaacg atcaatcctt cttttgcgtt caaattcgtt gcttttgctg
    22141 ctttaaccat ttcactgcgc aaatatgtgc ttgcttcata atatagaggc attcctggta
    22201 attgtcctgg tttgtagcca aatcctgttg catctgcatc aaaataagct actttgtctg
    22261 atataacgat gtcacctact tgtaagcctt caccgattcc gccagctgaa cctgtattga
    22321 tcaccatgtt cacgccgtat tgttggatca acagacttgt ggtaatagat gctaagactt
    22381 ttcctatacc ggaacgtacg acgatcactt cgtggttacc taatgaacct gaaataaata
    22441 aagcgcctgc gcgttcccat gataatggtt cgcttaggtt ctctctcaaa atttttactt
    22501 cttcttccat tgcgccgata ataccgattt tcatcttttt aattccttct ttctatataa
    22561 atgcgattgc taaaaaaacc acacataaca ttaggataac aataataaga gatttcatca
    22621 aaaaagagtt ccatttggtc gtcttttccc gttcgctgat ccttgttttc gtgattggtt
    22681 tattcttttt gctttcttta cgataaaagc tggcaatctt tttttcttct tgctgataag
    22741 ctgtttctgc ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc
    22801 gaagttcgct tcgcgtaata agcggtcctt tggccataag ccgatccctc tactttcctt
    22861 tggtcaatag ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat
    22921 ctttcatcgc ttgttgcgcc tcttcaagag ttagatgata caactccagt acttcgtctt
    22981 catcttgtgc taaaggattc tcgaccttct ctaccccttg tgcatgataa atatgaagga
    23041 cttcatttgc aaaacctggt gaaagataca tcgaagtaag atggctcagc gacttcgcac
    23101 gatagccagt ttcttcttct aattctctag ctgctgtcat ttcgggattt tgtccttctc
    23161 ctggatcgat ttttccagct ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt
    23221 gtttgacaag aagcagacga tcttgttcgt caaaagcaat gatcccaaca ccgccaggat
    23281 gaaataccag ttctctcttc gaagttcctc catctggcaa acgaacttcg tctacagcaa
    23341 catcaataat ttttccgcta tagatttctt tgcgactgat tgttttttct tcaaattgct
    23401 cattgttcag catatcttct cctgctttct ttgtaatcat aactgctttt tttcattttt
    23461 caatttttct caaaaactcc ttattagttt accatagata aaccttagat tttctctaaa
    23521 tatagaaaat ccttctttag tccgatactc tttctagtag tccgtatgct aaaataagta
    23581 tgtaatcgaa taacgaaaag aattcaatac tcaacgataa atgacatgga gggtttatta
    23641 tgataaaacg aaattggccg tggcttctag cattggtctt gctatttctc tttggttttt
    23701 cttttgttct aggactttta ttgataggtg tagttttagt tttgtgggga ctttttggtt
    23761 cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact gactaacgaa cttgaaactc
    23821 actatgaaaa aagcggtatg acagcgaatg aaatttcatt ttttcgacaa acaatgaatc
    23881 aaacaaaaaa tgagatcaat caattgcaaa tgaatatgca gcagacagcc aaacttaaag
    23941 caatcgatct ccgccatgat ccagtcaaag cagcaaaagc tttattcaaa gaattggtga
    24001 aagaaccaaa ccgactgcat gaagcaagtc agtttcttta tacgcatctg ccgaatctag
    24061 ttgatttgac aaataagtat aatgagatca atgaccatga gatcaaaaac aaacagacct
    24121 atgaaaaact agaagaaagc gcgcagatca tcgaccagct gtcagcttta atcgcacaag
    24181 attatcaaaa attcgttgct gaggatctag atgatatcga cgtagagata tctgttgcga
    24241 aacagagctt aaagcgtgat aataaatatg aaccagaaca aaaggattaa ctcttttgtt
    24301 cataaaagaa gactcggaac attcagatga tgtctggctg tttcgagtac tttttctaaa
    24361 gtagagacat tcatgtaaga atcgagaaca gattcaacaa ggaggaattt tttatggatc
    24421 ataaaccgac tgaaacaaaa gatgttacac cagttaacga tacacttgaa gatttattga
    24481 acaacccgtt ttcaactcca acagatacgc tgaccacttc acaacaagcg gagattgatc
    24541 agctccaaga gcaacagaca gctgctcgtt taattgacaa actaccggcg gaacgccaag
    24601 aacaagctaa acagctagca gcaaaaatcg atgctaatga tgcacagtct gtcatcagct
    24661 acggatcggc tgcccaagca aaattaagtg aattctcaca atcgatgttg aatcatgtgc
    24721 aagcacaaga tattggtcct gtaggagatt ccttaactga actgatgtat cgtctccaag
    24781 aagccaatcc tgacgaactg cgtgccggag aagggaacat cttccagcgt gtcttcggga
    24841 aggtcaaaca atcgatctat gaagtgacag caaaatatca aaagattggt gcacaaattg
    24901 acaagatttc agtgaaatta gataaagaaa aagacggtct gctaaaagat aatctgatgt
    24961 tagaacagct ttaccagaaa aacaaagact actttgatgc actgaatatt tatatcgctg
    25021 ctggagaatt gaaaatggaa gaacttcaaa caacgatcat tccagaagcg atgaaaagag
    25081 cagaagaaac tggcgatcag atggatgttc agattgccaa tgattacacg caattcctag
    25141 atcgtctgga taaacgtacg catgatcttc gcttagctag acagatcacg atccagcaag
    25201 caccacaaat ccgtttgatc caaaacacaa atcaggcctt ggctgaaaag atccaagcat
    25261 ctatcgctac agccattccg ctgtggaaaa accaagtcgt gatcgcattg accttgcttc
    25321 gtcaaaaaga tgcagtaact gctcaacgcc aagtctctga aacaacaaac gacttattga
    25381 agaaaaattc agaaatgctg aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg
    25441 gaatcgtgga tatcgagacc cttcaaacta ctcaaaatga tttgatcgaa acaatccaag
    25501 agacattgcg tatccaaaaa gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg
    25561 gacacatgga agaagatctg aaacaaaaac tgttggattt aacccaatag atcacctttc
    25621 gcttgttact aaaacaaacg cccaacaaca gtttattaca actgttgatg ggcgtttttc
    25681 ttatctttct tctaacttct ccattggagt ctatttctca ttcatcttgc tgtgtccata
    25741 gccatagcca aaataaatga tcagtccaat aaccattacg ataccgaaca cgatccatgt
    25801 cacagtttgc agacgaagca tcaatacaat acaagtcaag atggaaatga ttggcaatag
    25861 cggaacgaat ggcactttga attctccagc ttttggtttg ccaaacattt ttctcaactt
    25921 cagtaagcct agactaacaa gcatcaaata ggcgagcgcc atgatattcg ttagttccgc
    25981 taatacattc aatgggaaaa atccggcaaa gaatacagct gccacaccag cggcataagt
    26041 cgcttttttc ggtgtacgat gcttttgatc gatttctgtc ataaacttag gaagtaagcc
    26101 atccttgctg acagcaaaca gtaaacgagc aagtgaatac atcatcgaga ttgttaccgt
    26161 cagtagcgtt aaaatggccc ctacagcgat caccgcgccc actgtcccat gtcctacata
    26221 cctcattgca aaagccactg ggtcattgac tcctagttca gtatacggaa cgattcctgt
    26281 caagatcagc gtcacgatga tgtaaaggac agtagcaatc agtatcgaac caatgattcc
    26341 tcttggcaca tctttttgcg ggtttttgac ttcctcggct gccattgcaa cggcatcaaa
    26401 tcccaagaaa gcaaaaaaca caagagctgc accatctagc acaccggaaa aaccaaaagg
    26461 catgaatggc tgccaattat caggtttaac gaaaaatatt cctacaatga taaacaacgc
    26521 gatgatacca aatttgacat ataccatggc attattcaaa cgtaatgctt tcttggcttc
    26581 aagcgacacg atgtaagtga cgaatatcac tacgagcacc gcaatcaagt caatgtatgt
    26641 accattttcc ggattgtacc cgccagttaa tgcttgtgga agttcggccc ctaaactttt
    26701 caaaaaacct tgcatatagc cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa
    26761 ttccccgatc aacaaccacc ctgtcagcca ggcagcaaat tcaccaaata caacatacag
    26821 ataggcatat ggcccaccaa tcactggcac tcgcgaagca aattctgcga aacttagtcc
    26881 agacaaaatg acaacgattg cagctaagac aaatgaaaat gacagtgctg gaccagcatt
    26941 ttgattggcc gcaacccctg tcaccacaaa tattcccgta ccgatgatcg caccaattcc
    27001 aagcatgatc aaatcagacg tttttagttc ttttttcatc tcactcgtat gattgagatt
    27061 gatctctttc ttacgaaaca aatccattga agttcctccc cattttaggt tggaaaacag
    27121 cgattaaact gttttcatat accctaaaag gataccacct aaaatgacta aaaggcaacc
    27181 aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc
    27241 taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga
    27301 tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt
    27361 tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat
    27421 cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat
    27481 gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa
    27541 atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata
    27601 cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt
    27661 ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga
    27721 caatggtaac cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc
    27781 aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg
    27841 actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt
    27901 ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa
    27961 catagggact aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag
    28021 aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct
    28081 aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc
    28141 cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat
    28201 aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct
    28261 tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa
    28321 ggctgactcc ttgtgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat
    28381 atttgatggc attactaagc aattggttga agataaaagc gatccattta gcatcggtca
    28441 aaacagtatg atcctcacca acaatctcgt agcgaatatt ttttgggata aaataatttg
    28501 cctgactgcg tatcacaggc tgaatgattc cttttagcct atattcttgg atgaggtagt
    28561 cccgcgaaaa actgtctagt cttgcataat acaaaacttg ttctacgtat tcatcgattt
    28621 ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac
    28681 gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat
    28741 aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga
    28801 tcagctgatt gatatattct tctaccagtt tttcttcttc tgttcgtcca ccgctcaaat
    28861 agttttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc
    28921 tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag
    28981 caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg
    29041 caataaaaaa cagtcatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat
    29101 tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat
    29161 atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa
    29221 tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact
    29281 taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa
    29341 atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt
    29401 gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt
    29461 gatttttgca atcagtacat cgatttggaa aggttttgtc acaaaatcat ctgcccccat
    29521 gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac
    29581 tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat
    29641 atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc
    29701 ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat
    29761 gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct
    29821 tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat
    29881 tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcttttgt cttttcaata
    29941 cagcttaaat ttattatgtt attcttaaca attggtctat gatctgttat ttttccttag
    30001 ttgtttttgt cttttaaatt tccaataaca ccaataaccg aaaagcgtta gcactagcaa
    30061 tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt
    30121 tgaagagact tcttctgtaa agggaactcg ccttcctgtc actagtaatc ggtgagtgtt
    30181 cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc tgattgctaa
    30241 tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg
    30301 accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag
    30361 ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt
    30421 tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc
    30481 atcaaataaa ggcaagctaa cattgatttt cggaatatag attgctccga tcatatgttg
    30541 ttcgtagtat tcttttcctg gatttcggac atttctaacc gcttgctcaa aaggatcttt
    30601 gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg
    30661 cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt
    30721 ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa
    30781 aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg
    30841 ggtttgaggt ctattacctg ttctcttttt cattttcttc accttcttat ttaaaaaaac
    30901 tgcagcaatc ggttttataa aggttgctgc agcttatttt tgttgcttat acttcagact
    30961 tcattttaaa tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta
    31021 agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta
    31081 ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat
    31141 aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacata
//
LOCUS       DAPBAB010000028        30718 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPBAB010000028 DAPBAB010000000
VERSION     DAPBAB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.028"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            311..1090
                     /locus_tag="RD444_001557"
     CDS             311..1090
                     /locus_tag="RD444_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548935.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            1136..2080
                     /locus_tag="RD444_001558"
     CDS             1136..2080
                     /locus_tag="RD444_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7548936.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2096..2875
                     /locus_tag="RD444_001559"
     CDS             2096..2875
                     /locus_tag="RD444_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDT7548937.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2887..3585
                     /locus_tag="RD444_001560"
     CDS             2887..3585
                     /locus_tag="RD444_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDT7548938.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3613..4377
                     /locus_tag="RD444_001561"
     CDS             3613..4377
                     /locus_tag="RD444_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDT7548939.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4422..5111
                     /locus_tag="RD444_001562"
     CDS             4422..5111
                     /locus_tag="RD444_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDT7548940.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            6338..7018
                     /locus_tag="RD444_001563"
     CDS             6338..7018
                     /locus_tag="RD444_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDT7548941.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            7047..8360
                     /locus_tag="RD444_001564"
     CDS             7047..8360
                     /locus_tag="RD444_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDT7548942.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            9166..9933
                     /locus_tag="RD444_001565"
     CDS             9166..9933
                     /locus_tag="RD444_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDT7548943.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            9960..10961
                     /locus_tag="RD444_001566"
     CDS             9960..10961
                     /locus_tag="RD444_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDT7548944.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            10984..12270
                     /locus_tag="RD444_001567"
     CDS             10984..12270
                     /locus_tag="RD444_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDT7548945.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            12304..12957
                     /locus_tag="RD444_001568"
     CDS             12304..12957
                     /locus_tag="RD444_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7548946.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            12990..14195
                     /locus_tag="RD444_001569"
     CDS             12990..14195
                     /locus_tag="RD444_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7548947.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            14325..15650
                     /locus_tag="RD444_001570"
     CDS             14325..15650
                     /locus_tag="RD444_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548948.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            15667..16797
                     /locus_tag="RD444_001571"
     CDS             15667..16797
                     /locus_tag="RD444_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548949.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            16823..17908
                     /locus_tag="RD444_001572"
     CDS             16823..17908
                     /locus_tag="RD444_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HDT7548950.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            17931..18746
                     /locus_tag="RD444_001573"
     CDS             17931..18746
                     /locus_tag="RD444_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548951.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            18730..20448
                     /locus_tag="RD444_001574"
     CDS             18730..20448
                     /locus_tag="RD444_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HDT7548952.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            20474..21310
                     /locus_tag="RD444_001575"
     CDS             20474..21310
                     /locus_tag="RD444_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7548953.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            21315..22748
                     /locus_tag="RD444_001576"
     CDS             21315..22748
                     /locus_tag="RD444_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDT7548954.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
     gene            22786..24426
                     /locus_tag="RD444_001577"
     CDS             22786..24426
                     /locus_tag="RD444_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDT7548955.1"
                     /translation="MRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYLYDQYKDV
                     CSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCDEPVIINF
                     ADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDDGKKHKLF
                     SGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNWFDIGHAD
                     KYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVEYVRPRIF
                     EYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKRYSVSGDN
                     IQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALLSVSVPRE
                     LFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELAKLFHSVD
                     GKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDLKKIELIE
                     ALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
     gene            24514..24912
                     /locus_tag="RD444_001578"
     CDS             24514..24912
                     /locus_tag="RD444_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDT7548956.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            24937..25653
                     /locus_tag="RD444_001579"
     CDS             24937..25653
                     /locus_tag="RD444_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7548957.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            26885..27464
                     /locus_tag="RD444_001580"
                     /pseudo
     CDS             26885..27464
                     /locus_tag="RD444_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            27524..29176
                     /locus_tag="RD444_001581"
     CDS             27524..29176
                     /locus_tag="RD444_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDT7548958.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            29520..29885
                     /locus_tag="RD444_001582"
     CDS             29520..29885
                     /locus_tag="RD444_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDT7548959.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            29973..30416
                     /locus_tag="RD444_001583"
     CDS             29973..30416
                     /locus_tag="RD444_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548960.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
     gene            complement(30571..>30718)
                     /locus_tag="RD444_001584"
     CDS             complement(30571..>30718)
                     /locus_tag="RD444_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7548961.1"
                     /translation="EKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRY
                     SLGY"
BASE COUNT        10004 a         4479 c         6589 g         9646 t
ORIGIN      
        1 tattattaat tcaatgttaa tttttttgtt attttatttt ttgtgtaact tttaaaaaca
       61 cctatgaaaa ttaaggaaat aaaaatgaaa tcgattaatt gttatcttat taatgtttta
      121 tttactttta aatcataaat tgccctagtc aagaaaacag tttgtggtat gattatatag
      181 ggaaaacttg tttacaaata tttctgtgaa tatattagtt taattatgtt ttttaaaata
      241 aaaagctgtc atttttatgt aagtgaatta tatcttaatt tatctatcat caatacttag
      301 gagtgaatct atggaaaagc cattcgtcaa gtcattgact gctgtggcgg taggtgcagg
      361 tgctattgcg atctgtttgt ttggttacca tatcaataat cagcggcaac atcagcaacg
      421 gatcaattat gcagaatctg ccatcaccaa tcaaaaagac accttgactt ctttatcaaa
      481 agaagtagac aagctgtata gtacaaaaga aaaaattttt ctaaaccctg aaataacaga
      541 agaaacaatc acaagtttgt caaataagct atcctatgtc aaactttcag cagatgatta
      601 cgacatcaaa gaatcagaat tgcctaaaga agctgttgca atccaagatg agaagaaagc
      661 aatcgtgaca cagttagata gtgcagaatc aaaactgaag atccagacag ctgtcaacga
      721 tttgtttact aaaaatgtat caaactggca gcaagctgta gacgatgtca tcatcaaaga
      781 aaaggtgaca agtgcagatg tcgctcgtgt acgtgaaaat atgagctttt ttaaagatag
      841 tccttggaaa acggttgtta tgcaatattt aggattcgct gatactcaga tcgcgcaagt
      901 cacgcaacta gatcagttat tcgatacgat gttgaaggat ggtcaagtga ctgccaccgc
      961 tacttacgat caatatttga cggcgctcag tcaaatagag cagatccgga atgaaaaaat
     1021 aagcgcagca tacgcaacaa aagcacaaac tgttgctcag caaatgggct atagcaatat
     1081 aagttattga cagttaaaac gttgattcaa gcaaggttag caaacggagg aaactatggc
     1141 aagacgaaga agaaaatcaa gagggaaaaa aatatttctg attattttag cagttcttat
     1201 cgtattagta ttagcaattg taggagtagg agcaaaatta tatctggatc tctcacattc
     1261 tattgagaaa acttacgaat cagtggaacg aacccaagga gaacgaacga aaccagttga
     1321 tctggacaaa caagaatctt tctctgttct attgatgggg attgatactg gagatttagg
     1381 tcgggtcgat caaggaagat cagatacgat gatggttgcc atagtcagtc ctgaagacaa
     1441 acagacaacg atcgtcagca ttcctcgtga tacgtatgtc gacatcgtag gtcgtgacac
     1501 aaaagacaag atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt
     1561 acaaaaatat gtagacatcc caatcgatca ctatgtctct atcaatatga aagggctaaa
     1621 ggaacttgtt gatgcagttg gtggtatcga agtcaataat gacctcactt tctcacaaga
     1681 tggctatgac tttacaatcg gtaaaatcac attgaacggg gatcaggcat tatcttattc
     1741 tcgtatgcgt tatgaagatc caaatggtga ctatggtaga caagagcgtc aaagaaagat
     1801 cgtagaaggt gtagcaaaaa aagtattgag tttaaacggt gtcagcaact atcaatccgt
     1861 tttgaatgct ctagagtcga atatgaaaac agacatgagc tttgatgaaa tgaaaaaaat
     1921 cgctttagat tatcgggagg catttggtac gatcaagcaa gatcagctgc aaggagaagg
     1981 ctttatgcaa gataatatct cctatcaaaa agtcagtgat caagaacttg atcgtgtaaa
     2041 aaaagaatta aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga
     2101 agagacaatc aacttggaag aaatttttga aacattacgc aaacacttgg taacaatcat
     2161 catctcaatg tttgctggct tggccatctc aggaatcgta acatttttca tcttgacacc
     2221 aaaatacagt tcacaagcac aattgatcgt gacactgcca caatcagaca cagcaaatgt
     2281 caatgatgtc aatacgaacc tgcaaatgat caatacgtat aaagatatga tcgtcagtga
     2341 tttagtacta aatgaagtca aagatcgttt agaaacagaa gacaatgtga aaatgactgc
     2401 tggacaaatc aaagatgcga tctccgtcaa tcagtcacaa aattcacaaa tgttctcgat
     2461 ccaggcaata agcacgaatg cagtgactgc tcaacagatc gctaacacaa cttcacaagt
     2521 tttccaagag aacgcaaaag acgtgatgaa tgtcgataaa atctcgatta tttcaggtgc
     2581 agttgccaat acgacacctg tttcgccaaa taataaattg aatctagtga ttggcttagt
     2641 attaggaatg atggtaggcg taggtctggc attcttattc gaattacttg atcggacagt
     2701 gaaagatgaa aaatatgtga cggatgcatt aggattccca atcttaggaa cagttccaga
     2761 aatgtcagcg aaagaattga acgctggtat ccgtaaaaaa ggaacagctc ctgcacgacc
     2821 aaaagctaca aacgataaaa cagccccatc acgcagaagc cgctcaagag tctaggagga
     2881 agaaaaatgg cacgaacaca gaaacaaaaa gcagcacaaa ctcatgcagt cagtttgatc
     2941 acattaacaa acccatcttc accaatcgcc gaacaatatc gtacgatccg gacgaatatc
     3001 cagtttgcct ccgcagctgg tcagcaaatc aaaacgattg tcgtgacttc atcaggtcct
     3061 ggagaaggaa aatccaccac tgcagcaaac attgcagtcg tttttgctaa atccggtcaa
     3121 cgagttctac tagtcgatgc agatttacgt aaacccgtcg tctataagac attccagctg
     3181 aacaatgcca ggggattaag tacagcactt agttcatcag gaagtgtagc agatgtaatc
     3241 caacgaacgc cagtcgagaa cttgtcgatt ttgcctagtg gacctaagcc acccaaccca
     3301 tcagaattgt taagttcgcc aagaatggat cagatcctag acgaagcaag acagttattt
     3361 gacgtggtga tattcgatat gccaccagta gtagccgtaa cagatgcaca gatcatgtct
     3421 tcaaaaacgg atggcacttt attggtcgta cgtgaaaata catcacgtaa agaatcattg
     3481 aataaagcaa aagaactgct agagatggta caagcacgag ttttaggtgt tgtatacaac
     3541 ggagcagaac atagcaaaga tgctggttat tactacggaa attaaggaat aagaagagag
     3601 gagacaagag aaatgatcga tctacattgc catatcctac caggaatcga tgacggggca
     3661 gaaaacttag aagcaagtat tgcaatggct gaaaaagcga ttcagcaagg catcacgcat
     3721 attttatgca caccacacca caataatgga aaatacagca atgaaaaatc acaagtcatt
     3781 tctctagtcg cttcgttaca agccgaatta gagaaaagac agttgccttt gacgttattg
     3841 gaaggacaag aagtacgtat taccggaacg ctcatcgaag acatccaccg agatgaaatt
     3901 ttatttacag atttagatga tacatatctt ctgatcgaat ttccgacact ggaagtgccg
     3961 ttatatgcag aatggctttt ttttgagttg ttagaactag gaaagacacc agtgatcgtc
     4021 catccagaac gcaacgcgca ttttcgagaa gacccaaatc atttgcttcc gttcctagat
     4081 atgggatgtt tggcccaaat tactgcgcca agttatgttg gtgtatttgg caaagacatt
     4141 cagaaaacag ccaaaacaat ggtcaaacat aatctcgtgc agatggctgc ttctgatgcc
     4201 catggggtct ctaagcgtac cttttactta aaagagtgct atgagcagat tgctaaggac
     4261 tttggtaagg aaaaagtagt acagatgaaa caagtcgcaa aggatctgat caacggggat
     4321 gaaatcagtt atccagtcta tgaagaggta aaaaagaaga agtttggttt attttagttc
     4381 tttattcgta gataaaaagg aaatgaggag agagggaaag tatggaaagt gaagatttat
     4441 tatctcgtat tagtgaagta catcctaatc aagtttggtt ggataaagaa aaagttgaaa
     4501 aacgatatat ctatttattt tttaaaagaa tgattgatat tgtaggaagc attattggtt
     4561 tgattttagc ttctcctttg atgttctatg tagcttatcg aatcagaaaa gaagagccag
     4621 gaagtccgat atttttttgc catacacgtg ttggtaaaaa tggaaaaaca ttcaaaatgt
     4681 ataaattccg ttcgatgtgt ttagatgctg aagaaaaatt ggaagaatta cttgatcaaa
     4741 atgaaataga aggtgcaatg ttcaagatga aagaagatcc ccgtgtcact aaaataggta
     4801 aaattatccg tgccaaaagc attgatgaat tgccacagct atggaatgtt ttaaaaggcg
     4861 atatgagtct aattggtcca cggccacctt tggagaggga agtagctgag tacacgagat
     4921 atgatcttca acgcttgtat gtaaaacctg gttgttcagg tttgtggcaa gttagtgata
     4981 gaaataatgt tagtttccat aaaatggttg agttggattt agagtatatt agtcatcgaa
     5041 gtttaatgta cgacttgagg ttgattttaa aaacgtttta tgttattgtc aagcccaatg
     5101 gagcatatta gttagggata gaatgatgga ttgttaataa aaaagattac acatgttcat
     5161 attatcgttt caaaagtaat aactgtaatt atggagggtt tgaatcactt gtagaaaatc
     5221 taaaaaaaga aaggtgaatg gggcaattca gtatcattat tcttatataa aagaaaatat
     5281 tcgaaatttt gggatgatac cactcttaaa tataatgatc taaatgcttt aatattggta
     5341 tatccgatat aggaactgac gagctattta gtatgataca aaagtatatt gatagccatt
     5401 aataaatttt ccaaaaaata atgacagcac accaattttt ataatttagc ttgttgaatc
     5461 ggtatgttca tgggacggtt caaaaaaaga atcgaaaaaa taaacggtaa aattttagtg
     5521 aatcctatgt aataaaattt ggatagatgg aaaaacagca cataaggagg ctgagtttgt
     5581 tgtcatttct gctcctacaa actatggtag tgaatgtgtt attgaattag tgatgtctgt
     5641 tacttcagag acattgggga taattaactt cactatacct gtagggtata caaaaaatgg
     5701 aagaaaaaat attatcaata gattttattt agttcagaat ttttacgtgg aagtaaatta
     5761 ttgatgataa tctttatcca attagaatta ttatcgtagg atgtgatgat gatagccgtg
     5821 aagttgttca agtatttacc aatgtctaca acgcagaaaa ttagtttggg tttttcggat
     5881 tggaaaattt caatgtataa aatcataaat ttgctgtaaa tagacagttt ggggtagtaa
     5941 tagcccaata aattgttcga taaactcatc tttggggtga gaatagctca atcattgaat
     6001 ttcaaacctg cctttggggt gggattagag gtcaatatgt acctacataa tactttttgg
     6061 aaaatgatgg tgttgagaat gtaagtacat ttcaatgtga aattctaata tagccccaaa
     6121 cgaggtcaat aaaaatgacc taaactcgta tttctcccct aaacgaggag gagatagata
     6181 tgaaaattga atgtgccggc gttggagttg gctgctttta gtaggaatga acatggttcg
     6241 atgagaactg tttcagatag agtggtcgat gatcacaggc ctttaagtcg tttacagatg
     6301 atgagacttg taaaaataga gggacgaatg gaaaaagatg attggtatag aagaaaagaa
     6361 aggtgttcag aaagacgtaa caattgatag ggaagagaat cttaggggac atagattcta
     6421 ttggattgga cgcagagcac aagatatctt cttctcggct ctagctttga ttgcattgag
     6481 ccctgtgatg ctaattacag cgctcgccat tgtgattgac gatcccggtg gcagcccgat
     6541 ttttgtgcag gatagagttg gaagaaatgg aaagctgttc aagttttata aattccgatc
     6601 tatgtgtgta gatgctgaag caaaattaga agccttgttg gcacaaaacg agatggatgg
     6661 tccggtcttc aagattaaag atgacccaag aatcacgaga gttggtaaat ttattcgaaa
     6721 gacaagtatc gatgagttac ctcagctctg gaacattctt aaaggtgata tgagtattat
     6781 tggacccaga cctgctttac cgagagaggt ggaacaatac ggtgactacg aaaggcagcg
     6841 gttgttcgta cagcctggtc ttagttgcta ttggcagatt gctccacatc gtaatgaact
     6901 gtcctttgaa gaatgggtgg aacttgatgt gaagtacatc aatgaaagaa gctttatcac
     6961 ggactggaag attatcttct tgactttaca ggctgtatta tttggatatg gtgaatgatt
     7021 atatatagta ttaggagttg aaatatgtga atattatttt attatcgggt ggtagcggta
     7081 ggcggttatg gccgctgtcc aacgatattc gtagcaaaca attcatcaaa ctgtttaagg
     7141 atgaaaatgg cgaatatgaa tctatgattc aaagagttta tcgccagatt acaactgttg
     7201 acccagatgc aaaggtcact attgcaacaa gtaagtctca ggctagtgca atccataatc
     7261 agttgggaga taaggcttcg atttgtgtag aaccttgtcg tagagatact ttccctgcaa
     7321 ttttgttagc tgcagcatat cttcacgatg aacttggcgt aggtgatgat gaaagtgttg
     7381 cggtttgtcc tgttgatcct tatgtagata attcctatta cgaaggcgtt gaagcccttg
     7441 agaagttggt gcagcagaat acggctaatt tgactttgat gggtattgca cctacatatc
     7501 ctagtgaaaa gtacggttat atcatccctg aaagttctaa cgaagtaagt actgttaagg
     7561 agtttaagga aaaacctgac aaagcaacgg cagagaaata tttagctcag aatgctgttt
     7621 ggaatgccgg tgtatttgcg ttcaaacttg gttacctgtt ggaaaaagct cacgcaatgg
     7681 tggattttga agattatcgt gatttgtatt ccaagtatga gactttaacc aagatcagct
     7741 ttgactatgc tgtggtcgaa aaagaaacga gcattcaagt gatgcgttat aacggggatt
     7801 ggaaagatgt cggaacttgg aacatgatgg ctgaagttat ggctgataat attaagggga
     7861 aagctgtaat ggatgatacc tgtgttaata ctcagattgt gaatgagttg aatattccta
     7921 ttcttgctat gggctgcaaa gatatggtaa ttgcagcaag tggcgatgga attctgattt
     7981 ctgataaaga gcgcagtggc tatatgaagc cgtatgttga gaaaatcagt acggaagtaa
     8041 tgtttgcaga aaaatcctgg ggtacatata ccgttatgga cgttcaggaa ggctccatga
     8101 ctgttaaagt ttctatgcag gccggacaga agttgagcta ccactaccac aattctcgcg
     8161 atgaagtttg gactgttgta gcaggacagg gtaaagcgat tgtagatggt atgaagcaga
     8221 ttcttcgtgc gggtgatgtg gtgactattg ccgcaggatg taaacataca atcgaagccg
     8281 tgacaaatct ggatattatc gaagttcaga ttggtgaaac tattagcgtc gctgacaagt
     8341 ataagagttc tttgaactaa aagttcgcga ggtagttatt atggagatac taaaatttca
     8401 acccgtgggt aaagattatc tttggggtgg agaacggttt aagacggaat ttggaaaaaa
     8461 ttgacttaac accattaagc ttatacgtgg gaatgcttag ttcatctaga cgataactac
     8521 gtacggaaat atcaaatatg aaggacagaa tggtaaaacc gagttgtggt atgtattgga
     8581 agctacaccg gggggtgatt tatgcttttg agtacgatgt aacagaagaa atgttacgaa
     8641 atgctgtgat ggacagatca cttgggaaac atttatataa agaagaagta cacaagggag
     8701 atctttttta tgtcagttca tggtattggc gctggagtat tagtggctga aatacaggaa
     8761 agctcaaatg ttacctatcg agtttacgat tatgatcgag tagataagaa tgataataag
     8821 tgagaattgt actttgaaaa agcgattcag gtaatgaata tggaagccac tttgaaattg
     8881 gagaaaagaa ataagctcat tcatttttat cctggtggct ctcgtgaaat actgtgtaga
     8941 tgtaaatact gtgagcccga aagaatccaa gtaaagacgg ggttttcgtt ctcggtaaaa
     9001 gaggaatctt ttcaagttct tttatgtata tcgggggacg gacaagttga gtcggcaaat
     9061 ggtgttatga aactcattag atttgtaaaa aagatattgt atgtttttgc caacgaatat
     9121 tggaagatgt taagtgttga gcgatgcgga cttactgaaa attagatggg gatagaggga
     9181 ggatatctaa tgtcacgaat gagatttatg aacacagaaa ttgacaatct tacaatgggt
     9241 gaagtattgg tagaggttga aaaaatgatt caagatagga gatgcggata tgttgtaact
     9301 ccgaatgtgg atcacattgt tcagttggaa acaggaaaag agttgcaaga tgtttataaa
     9361 aacgcatctt taattttgac ggatggtaaa ccattgattt ggatttcaaa gttttataag
     9421 acaccaataa aagaaaaaat atcgggatcc gatatatttt ctttactgtg caagatggct
     9481 gcagaaaagg gatacacaat gtatttttta ggtgcggcag agggcgttgc ggctaaagcg
     9541 gcagataacc tgatgaaaag atttccggga ttaaatgttg taggaacata ctctcctcct
     9601 tatgggttcg aaaaagacga agatgaaatg tttaaaattg attcccaaat taaacgagca
     9661 aatccagata tgcttattgt gggtttgggt tgccctaaac aggaaaaatt tatgttttat
     9721 aactgtgata gattgggagt acctgtgtct tttggtctag gagctagttt ggactttgag
     9781 gcagggaatg ttaaaagagc accaaaatgg atgtcagatt atggatttga gtggctgttt
     9841 agaattactc aggaccctaa aagaatggca aaacgatatt tggttgatga tttaaaaatc
     9901 tttaaattag cactaaaata tcggaaaaaa taaactatat tcaatgagga caatgaagta
     9961 tgattattac aaaaacacct tttcgcatgt ctttttttgg cggcggaacg gatatggaag
    10021 attattttcg agaaaatggc ggtgcagtgc tttccactac ttttgataag tactgttatg
    10081 tgaatgtgcg tcatttgcct agattttttg attacagcac agagttatca tattctcgta
    10141 ctgagagagt aacaaatatt gaagatatcg agcatcctgc aattcgtaat gcgatgaaaa
    10201 tgctagatat gcatgaaatt cgccttacat atgaagcaga tcttcctgca cgttctggtc
    10261 tgggtacatc tagttctttt gccgtaggta tgttgaatgc tttctatgct ttgaagggca
    10321 agtacgctga caagaagaaa ctcgttgata aagcaattta tcttgaaaga gtcctttgta
    10381 atgaagcagg cggatggcaa gatcagattg cagcagctta tggtggcttt aaccgaatca
    10441 atttcaactc agatggctat gaagtattgc ctattattat tacgcctgag cgtaaaaagc
    10501 agcttaacaa caacttgatg atgttcttta ctggattcac cagattttcc tctgatgttc
    10561 agaaggcaaa taatgtcagc ggcacagagg acaaaagagt aagactgaaa aaaatgtatg
    10621 agcttgtgga tgaagcagaa gcggttctga ctgataagaa cagagatcta gatgatttcg
    10681 gcagactgct tgatctcact tggagattga aaaaggctac cggtggtgcg attagcactg
    10741 gaagtatcga tgaactttat gaaaagggca tggctgctgg tgcattgggc ggtaaacttc
    10801 tcggagcagg tggcggcggt ttcttggttt tttatgttca gcctgaaaaa caaaatgctg
    10861 tgcgttcggc aatgagtaat ttgatgtata ttccgtttga atttgaaaac ggtggaacac
    10921 aggttattca ttacacaccg gaaatgtata ttccaaaata aagaaatcag cgaggataag
    10981 aatatgaaag ttgttattat ggcaggcggt aagggaactc gcatctcatc ggttgcaagc
    11041 gatattccaa agcccatgat taaaattgaa aacaaaccgg tattggagca cgaaatcgaa
    11101 tgtctgagag accagggatt cacagatatt attcttaccg ttagccatct tggcgatatc
    11161 attatgaact attttggcga tggttctgga atttcaccag ttactggtca gccctttgac
    11221 gttaaaattg aatacttttt ggagaaagag ccattaggaa atgccggtgc attgtttcgt
    11281 ttaaaggata atttgaccga agacttcctc ctgctgaatg cagatgcgat gtttgacgtt
    11341 gatttcaacc gatttgtagc atatcaccgt gaaaagggtg gcttggtgac attgtttact
    11401 caccctaaca gtcatccata tgacagcggt ctaatcattg cagataagga tggctctgta
    11461 gacagatggc ttgtaaagga agattcccgt ccaacttatt accgtaacag agtaaatgcg
    11521 ggactccatg tgattagtcc tgctgttctt gatgtcgaga taaacacacc taagattgac
    11581 ttggaccgtc agttattaaa gccgttggca ggaaccggaa aaatgttctg ctatgatagt
    11641 ccagaatacg taaaagacat gggtacccct gatcgttatt atgctgtctg tgaagatttt
    11701 aaggctggaa gagtgaaggg caaaaatctt cagaataagc agaaagctgt tttccttgat
    11761 cgtgatggaa cgattaatca atatgttggc tttctgcgtc atattgatga gttcgaacta
    11821 atcaagggta ctgaagatgc agtgaaaaag atcaatgccg ctggatatct ggctattgta
    11881 ataacgaatc agccagtaat tgcaagaggt gaagtttctt ttgaagaatt ggaagagatc
    11941 cataacaaaa tggagacact tcttggatat aagggcgctt atttggatgc agtttattat
    12001 tgcccgcatc atccacataa gggatatgag gaagagcgcc cagagctaaa attcgattgt
    12061 gaatgcagaa agccaaagcc cggaatgtta attcaggcag ctacggactt caacatagat
    12121 ttgagtcagt cttggatgat tggcgatggc gagaatgata ttaaagcggg ccaagccgcc
    12181 ggctgtaaga ctgcattggt tggcgagagt gagtatggac aggatatgac catagcaacc
    12241 ctgagtgaat ttgcagataa gatctgctaa gggatgtttt atcacgaaag gagagtataa
    12301 acaatgatag acaatagatt acagaaacac attgatttgc tgattaaacg ctatcccact
    12361 cttgaagtaa tcaagcagga tattgttgac ggatacctga ttatggagga atgctattta
    12421 aatgatggca agctgctgat agcaggaaat ggcggatctg ctgctgattc cgagcatatt
    12481 gcgggtgagc tgatgaaaag ctttaagacg cctcgccctg ttagtaggga atttgcagat
    12541 aaattgattg cggttgatcc tgtaaagggt tctgaattag ctaagaatct tgaatatggt
    12601 ttgatggcga ttcctctcgt tgctcatgaa gcgctttcca ctgcgtatat taatgatgtt
    12661 gatggcctag gtgttttcgc acagcagttg tttggatttg gaagacctgg cgatgtattc
    12721 cttggtattt cgacatccgg caatagcaaa aatgtgataa atgcaactgt tgttgctaga
    12781 gctcttggta tgaaagtaat tggcttaact ggagccaaag gtggagaact tgcaagggta
    12841 gcagatgttt gtgttaaagc tcctgaaaca gaaacatata tgattcagga gtaccatttg
    12901 ccaatttacc actgttggtg tctaatgttg gaagataaat tttttgggaa tagataaaat
    12961 atctaagccc atattgaaag gataaaatta tgaatgtact atttataaca actcaatttc
    13021 catatcctct agataatggc ggaaaaattg gtgcgtacaa cggtatcagt gttttatcta
    13081 gagactacga tgtgacggta ttgtctttca ccgaagaacc gcagtatgtt gaagaaggtg
    13141 tcagtttttt tcgagagaaa tttaatagtg taaaatttga aaatcctgtg atacatgatg
    13201 tacacataag aaataatcca atgaaattgg tagaagcttt agcaataggg tattgtctac
    13261 gccaacctta tatagtttca aagttttatg attcaaaaat gctgcagtta atagatgaaa
    13321 aattttctaa caatgcatat tgggatatca tttttattga ctatctgaat atgcaaagtt
    13381 atggtcaata catacgggag aagtataaaa cacaatttgg taagtatatc ttaaaagacc
    13441 acaacataga atatgattta gtcctacagg aagctgaatc aagtcgaggt ttaaaaaaga
    13501 agttgcttca gttagagtat aagcgtactc gaaagtatga agaggccgca gtaaataggg
    13561 ccgattattg ctttagtgtg tgtgatgcaa atacggaagt actgaaaaag attaatgaca
    13621 acagttatac gatgcttcca acttatgaaa tgcttacagg ggtgaaaaaa aatatagaga
    13681 agaacacgat cctatatttg ggtaaccttt cttggaaatc caatatgcaa ggattagttt
    13741 ggtttttaga gtcaatttat ccaaatattc aacaaagaat tccggatgta aaaatcacaa
    13801 ttgtcggtag cgggccagtt ggaacagatt ttagtaaata ttcaggcgtg gattatcgtg
    13861 ggtatgtaaa agatatttca cacatttatg acgatcaagc ggtgctaatt gtacctttgt
    13921 tagaaggtag tggtataaga ataaaaatat tagaagcgtt caataatgaa attgcagttg
    13981 tatctacatt agttgggtgt gatacgattg gcgcaaaaga tggaagtgag ttatttatag
    14041 cagataacga aaaagatttt gcaacggctg tagttagact gcttgaagac atgaatttga
    14101 atgaaaaagt aagaaagaat gcaaaattat tcttaaaaga aaaatactcc ttaacggcta
    14161 gacaagatga gtttagtgcg ataattaaca gctaatatat atataagctt atctgatgat
    14221 gatttatagg aggaaatata tggagatggc tgagagaaag gaaaagaaac ttggaaataa
    14281 gaatatcata ttattttaga aaactcctgt tattttatgt ttgtgtgatt gttgcggcta
    14341 tgagttacca ccttacaggc aattccaatg tagcgtcatt aattgctgta gtaggagtag
    14401 tttatgctat tgtaaaggcg tgtaataacg agtatctggc tatatgggtt atgtttgtat
    14461 taggttctga tatatggggg atagtgccat atattaacat tggtagtggt gtgttgcatt
    14521 ggtcagattt taatgttata gtagcgatat ttctctgctt gagattcatc aaagttaaag
    14581 ctaggagtag atacgggatt gtctatagtg taatgcttat atttattttg attgcatgtg
    14641 tgcaatcgca ttcaatctat ggacaagatg tacatacaac gctcttaaca gctagagaat
    14701 tgctagttgt aatatccttt tggccgatag taaaaatagt atattcagaa agaataacta
    14761 aaaatgaatt cattgattta gtgtgtaaat tcacaagatg gacaatgtgt atatattggt
    14821 ttgaatggat tcttgttaat atgggattca atataacctt tttacagaca ggtgcacgct
    14881 ggggtactag aatctatata gatccgatat ttatcatttt atatttcttt ataaatctat
    14941 atgcggttat atataaaaga gagggctata atagtacaaa taaaagtata tggaattgca
    15001 tttttagttt actgacattt gttataatct cgcaaggcag aagtggtata ttatatgtag
    15061 taataacctc ggttattatg cttttaattt cgcttaaacc atccaagatt attagatttg
    15121 gtatattagg aattgcctta attgcatgtc tagtgtctgt gacccaaatt agagaggtcg
    15181 ttatatcttc gtttgtagag tctcagagca gtgacacagg taatatcgca tatagaattg
    15241 ctgaacaaga ttattttgaa aataaattag aaggtcatga actatttgga gttggtatac
    15301 caaacaatca tgatgccaga gctgtagaat attcaggcaa aacaatagat tttgagtcta
    15361 aaaaatataa tccctattat cttgaggact taggcgcttt tgaaattcgt tatagatttg
    15421 gtataattgc atatgcgtta tatatagttg ctttagctat aagtattata gatgccggat
    15481 ttcgaagaga caaatcggtc gccgcatttg cgggtatggc attcatggtg tttatggcgc
    15541 ttaattttaa tttgctagcg tatattacag caagaccgtt cggatttatg atttctttaa
    15601 ttttgataga aatatcggca ttcaatgatg aattcatata tattgaatag gtaagggaga
    15661 gcacagatga atttagatcg attaaagaaa atagcaaata tgatttcacc aatcataaaa
    15721 gtgataaatg cgccgttagc gtattttagt gggaaatcag attatatgga gaagggaata
    15781 ttgtggccta ctgtaacaac ctactccaat aacagtagaa aagtgattca atatacaact
    15841 ttacctaaaa acgtagacaa catggctcaa atgcaatggg ctggggaaag ttttgaggac
    15901 atatgtatag ttcttcaagg tccagtatta gaagacggtg attttacact aaataccgtt
    15961 aaaatgtata ataaatacta tcctaaagta aaacttgtag tgtctacttg gataggatta
    16021 tctgaagaga cagaatgcaa gttgcgtgaa gctggtgcag atgttgtatt atgcaagaaa
    16081 cctgaaaaaa caggtgcgta caatattaat ctacaactta cttctgcata cgaaggcgta
    16141 aaaatggcgc aaaaactggg gtgtagttat gcgctgaaaa cgcgaactga tcaaagacta
    16201 tatgcaaatg atgtactaaa gtatttccaa acattattga atatttttcc atcttcggac
    16261 tgtaaagtag tgaatcagcg attaatattt acgacttatg gaaaatcatt tagatatctt
    16321 ccgtttaatt tatgtgattt tttggtatac ggagaaacta gtgaattatt aaaattatat
    16381 ggtattcaaa gagataaccg agcatcagac tttgggaaaa ttatttatac tgaggaaaaa
    16441 gagtttaata aaaagatatt tacactacta gaaggaaaac aaggtgtatc tccatatgat
    16501 gtttttccag attttgatgc actatataaa aaatatatgt ttgcagaata ctatattgtg
    16561 tataattatt tcctggaaaa tattgcaagt aatgaatctt atggtgactt aatggatgcc
    16621 tattatagtt atttaaagaa atatgcagtt gttgctgatt cagaaaaatt gttactatat
    16681 tggccgaaat atgttcaata taatgttaag ttcgaaaatg aacttgattt ctatgcgaag
    16741 ttggattcaa aaaaatggat ggatatttat gctaattata caccttttag tcagtgatta
    16801 tatggaaagg atgaggtgaa taatgaaaat cggaatagtg actattgtta atggggcaag
    16861 ttttggtaac agattacaaa attatgcgac tcaaaaagtg ttggaaaata acggctatac
    16921 agttgagaca attaataatt gttttgacaa agaaagtacc ctatccattg ttatcaaaaa
    16981 cgctatttat acattattta agtttatacc gcatttcaaa aagtcaaaaa gtatctggaa
    17041 aagaatgtat acattttatc aatgggacaa gcagtatata gattattctc atcgtaaaat
    17101 tgatgcatct aaaaaaaaca gctttgtgag tataaagaat gattatgact atttcttggt
    17161 tggtagcgat caaatttgga atccaaaata tgcagccaat aagggatttg aacttttatc
    17221 atttgttcca aatgaaaaaa gagtttcatt atcaaccagt ttgggagttc catctatacc
    17281 aagtgattta gaaaaattat atgtgacaga gtggaataag tttaaatcaa tatcggtacg
    17341 tgaaaatcaa gcggcaaaaa taattgagga cttgacaggc acaactgcgg ctgttcacat
    17401 tgacccaact ttaatgttgg agcgttcgga atgggaagcc tttcaaaaaa aaccagacaa
    17461 tattgatgaa aattacatct tgtcgttttt cttaactaat ccgcctaagg atactgttga
    17521 tgtattagat aaggtcgcta aagatttgaa aataggtgtt ctcaccttag atgcgtcttg
    17581 ggaatatttt caagatatta ctccgaatca ttttgtttat ttgatttcac atgcaagaat
    17641 gatttataca gattcattcc actgcacagt tttttcattg ctatttggaa aaccggttgt
    17701 ggtatttgac cgttttgata gtgaaaaaat gaattctagg ttaagtacat tgttaaataa
    17761 agtaaataaa gcacattttt gggtaggaaa aaaagctgat gaagcaatga ttcgtgcaat
    17821 agcatctgaa cctgaatatg acatagataa gctgatggta tcagagaggg aatgtgtaaa
    17881 agcttatctt aaagagtatt tacagtaaaa ttttgaaatg ggatgaaaat atggagcatt
    17941 taagtgattg ttttggtaaa atgaagattg ttacgacttc atgcgcaaga gaatatttta
    18001 aggatgattt ggataaatca gagtattcat gttttgttga ttctcgagca gctatgtttt
    18061 tcgcaatggg acaagcttta gctaaaagaa ggaatgtagt tgtaatgctt gctgatgaat
    18121 tgacatcgtg ttatacggca ctaacagaat gtgcatttca acaagttgat ttaattgtgt
    18181 ttgcttttca gacagtcaga catgaaattg atttagcatc tttgaagaat tgtggttgca
    18241 atctaattaa ctgtagcgcc caagacgata ttgatagagt tgtgtccaac actaaggggt
    18301 taaatcagac aacagtaatt aaaatcaata tgaattgtga gtcggataaa gatcatatat
    18361 gtgagtatat agacctgcca gacatacctg tttttttcca cggtagctta tactcacctg
    18421 caagtaaaaa gaaatatgag cagtatggac aggtatcgaa gtatttaggc tatctcgtgg
    18481 ggggaaaaga tgcaatactc ataatttctc acaatgagtt tttaaacgat atcagtgtat
    18541 ggattaatta ttattctttt ggagaaattc aacccaaaat tattatattt gacagtccga
    18601 atgactatca atataaatgg ctgtgtgact tagaatgcag tgtagctcat tcaaccaacc
    18661 gatttttgtc tgaggattta gccttattgc aaaataacgg aatttccatt gtgttaagag
    18721 gtgataatta tgatatcgtc agttaaaaat gttcaaattt tagtatcttt attaaaacag
    18781 cataatatta aagatatagt tatctcgcca ggtagtagag attttcaatt ggtgcatagt
    18841 gtagaaacag acagtttttt tagaacctat acggttgtag atgagcgcag tgctgccttt
    18901 tttgctaatg gactttctat ggcgattgat aagcccgtgt gtgtatgttg tacttcttca
    18961 actgcctctt caaattatat ggcggcagct aaagatgctt ttgctaatgg catccaactt
    19021 gtacttctta ctgctgatag agattaccgc cgcttatatc agatggaaga tcagatgatt
    19081 gatcaggtag atatgtatgg ggcttatgtg cgttctaacg ttgatattcc tattgttaag
    19141 gattctgatg atgaatggta tgctaccaga aaaataaatg aagcaatatt ggagttaaat
    19201 catggcgtta agggtcccgt gcagattaat tatcaattaa tggatttaag tgcgccgttt
    19261 gttgagacca tgccagagta tcgtaaaatt acaagatatc aagaggatga tttactgtca
    19321 tcagtagaga tttttaaaca gaaactatta tcaaaaaaga gaataatggt tttatgtgga
    19381 cagcaggaag aaaataattt aagtgcctta ttggaagagt tctttattaa atataataca
    19441 attataatca aggataacta ttctaatata cacggtgaac actttattaa gactgttcta
    19501 gtaacagaaa gaatgagtgt aaatgcattt attgattatt gcccagattt ggtaataact
    19561 attggtaaac atgtatggtc ttttgtgaaa tatactctta gagataaaaa gcttaaattt
    19621 gagcattggc ggatatctga ggatggtaag attgttgatc cgttcaacaa gctaacatct
    19681 gtctttcagt gctcagcgga attttttatg gagactgtca ctgctggtgt gaatagcaat
    19741 aataatcttg agtattataa tctatggaag acaagattat caaaagtttg ctatccagag
    19801 ttagtctata gcaattttta tgcaatttca gaactagtaa aaaaaattcc agatagtgca
    19861 ttagttcatt ctagtatttt aaatgcggct agattgtatg actttgtatc tgatgataaa
    19921 gcattactca gttttgctaa tttaggggct gatggtattg atggttgttt accaacattt
    19981 ttaggtcagg cagaagcaga taaaactcga cctgcgtatt taatttcagg agacttgagt
    20041 ttcttttatg ctatgaatag tttatgtggt gacattccag ataatgcaca tattttcctt
    20101 attaacaatc attctggaag tgaattccat acaaattttg gtaagtttta caacggatca
    20161 ttaagtgttg ataagcatat agctgcagga catatttcaa acgcaaagga ttgggcatta
    20221 gaaaataata taaaatacat tactgcggat tctaaggaaa gtctcaaatc ggggttggat
    20281 gtatttatta atacatttga cagtcctgtt ttatttgagg tctttacaga tccggatacg
    20341 gatacgtctg ttctggaatc tttttatagt ataaataagg taatcacacc aaaaatgtat
    20401 tttagaaagg cgttagatat tataaaaaag catttacaca ttaagtagat gtttattgcg
    20461 aggtgaaaca aatatggcaa taaaatctta tattaaacgg ttattgcttt atatccgtca
    20521 tggagatccc aaaataaatt tagatgtgag ttatttatct gaaggtgcaa tgcttgctgg
    20581 taagactgtg ataattacgg gtggtgcaaa aggacttggt tttgcaattg caaaatcatt
    20641 tatagctcaa ggcgcaagcg ttattattac aggaagagac atggattcgt tgaataatgc
    20701 taaatgtcag cttggagata tgtgttctat cagggcgttt gatataactg atgctaaaat
    20761 gtatcgaacg ttttttgaag atgcatacga gacctttgga cgtattgata tactgcttaa
    20821 taatgctggg atctcgttac acgaagatgg atttatgagt gttactgaga aaacttggga
    20881 taagcaaatg gatacaaatt tgaaaggtgt ttatttcttt acacaggaat ttattaagtt
    20941 tgttaaagaa aagaatcaaa tgcaagcaaa tattattatg atgtcctcag aaagaggctt
    21001 gtgtggtgat agtattccgt atggattatc aaaggctgca attaactctt tagttgtagg
    21061 cttgtctaag aaatatattt ctgagggaat tagggttaat ggtattgctc ctggagtaac
    21121 tgcaaccgct atgactggta tttctgaaaa gggtaatttg tataatcctg caacgatcgg
    21181 ttatagatat tttctcccag aagaagttgc tcaagttgct ttgtttttgg caagtagtct
    21241 ttcaaactgt atcagtggtg aaatcatagc ctgtgaccag gggcaatatt taagtcgaaa
    21301 ttgggggtaa taacttggga atattattaa aaaaatataa tagccttagt gagcctgcaa
    21361 aagcatctat ttggtttaca ctttgtaatg tgttgcaaaa aggaattcta tttttgacta
    21421 cacctatttt tacaagaatg ctttcaactg cagaatatgg taagtatacg atttttatat
    21481 cgtggtattc tattatagca attttcacaa cgttgaattt atctgcaggt gtctataata
    21541 aaggactaat taaatacgag aataaatatg cattaacctc gtcattttta ggattatcgt
    21601 catttatttc cctttgtgca ttgatgctat accttccatt tagaacggtt ataaatgggc
    21661 tactggattt atcgacggag tttgtcatat tgattcttat tgaatcaata tttgagccgg
    21721 cttttttgct ttggtcagtt aaggaacgat ttgattacaa gtatcaaaaa ctagtactca
    21781 tcacagttct tatggctgtt ttaaatccag gtctcggtat tatattaatc ttatcatttg
    21841 gggagtatgc tatttggaga gtacgagctt atgtcgtggt aaatgttgta gtggggatgt
    21901 ttattggcat aggaatactg gctaagggta agaaattatt tgataaagag tactggacat
    21961 atgcgctcag atttaatttg ccattaatac cacattattt atcaatgaca ttgctgaatc
    22021 aggttgatcg ccttatgata aatagtatgg ttggagctag cgaggctgcg atttatagta
    22081 tcgcttacac tatatctagt atgatgaata tcataacaac agctataaac ggttctttta
    22141 ttccttttac atacaaagca attaaagatg gcgtgtatga aaaaattaaa tccgttgcta
    22201 atcaactatt agtgttgatt tttgtcttgt gttcgtttac tatgctgttc gggccagaat
    22261 tgatttgggt tgtcggagga gaaaaatata tggatgctgt ttggattatt cctccagttg
    22321 cggcatcagt atttttcatg tttttatatc cgctttttgg caatattgaa ttttactatg
    22381 aaaagacaaa ctacgtaatg tttgcatctt gtgtgggctc tatcgcaaaa attatttcaa
    22441 actatgttag tattccccgt tttggatata tttcggctgg ttacacgaca ttgatttgct
    22501 atatgttgtt ttcctttgcc cactacatgt tttataaaaa aattcttaga gaaaatggta
    22561 taaaagaaga tatgtataat attaaaaata ttatatttct ttctgtctta atgattgtgt
    22621 taactattgg tatgacaata ttatactttt ctacaattct aagatatagt attattattg
    22681 gattagttat tgtggctttt cttaaaagga cgcaaataat gaaaatcctt aaaaatataa
    22741 aaaattaaca agcaaatctt gggaatggaa cggaggcacg gtgaaatgag attattgatt
    22801 ccatctgcta agattgtacc ggaagaactt caccatcttg gaaaacttcc tgcaattatc
    22861 tatccgatta atcaaaaaat tgtatttgat tatttatatg atcaatataa agatgtatgt
    22921 tctgctatcg atattgcctg ttacgaaaag atggataagg tagcaagacg attagataag
    22981 tatattaaat ctaaaacagt aaatattatt cagcttaaag agttagggga tcttggaaga
    23041 actatatatg attctctaat aggatgtgac gagcctgtta ttattaattt tgctgatacc
    23101 attatcaatg ataatatata ttccttggaa tgtgacagct ttttctatgc tgaagactat
    23161 tatagtaata cttggacatt ctttgaagaa aaagacggag atattatttc tgttttagat
    23221 aagaacgagt taaaagaaga tgatggaaag aagcacaaac tgttttctgg tgtatttcaa
    23281 attatggatg cccagtattt tagagagtgt ttgcgtaaag ctttgatgtc aaatgtagta
    23341 aatgtgaata gtttttatca agctttacaa gagtatagca aacgatatga atttctctct
    23401 attaaaacaa ataattggtt tgacattgga catgcagata aatattataa ttccaaattg
    23461 gaagttaagg caagagaatt taaccacatt tcaattgata aagatcgtag catcctgaga
    23521 aaaattagtg aagatgttga aaaatttatc ggagaaatta agtggtatct taagttgcca
    23581 gcacaggtag aatatgtgag acctagaata ttcgaatatt ctacttcgta tattaatcca
    23641 tatgtaagta tggaatacta ttcgtatcat actgttcacg agttatttct ctatagtgac
    23701 ttaacaaaaa aacaatggat agatatcttt aatagaatac gatttgtatg ctcagacttt
    23761 aagagatact ctgtttcagg ggataatatt caaaagtcat tgaaagatat gtatctcgat
    23821 aaaacatttc agagattcaa taagctcaga aaggaccccc ggttcacaga attcttttct
    23881 tctgatattc aaataaatgg agtcagatat aaatctttgg accagataga agctttattg
    23941 agcgtaagtg ttccaagaga attgtttgac attacgcaat ttaatattat tcatggtgac
    24001 ttatgctttg ccaatattat ggtagataac acattttcgt tcattaaagt catagatccg
    24061 cgaggtaagt ttggagattt tgatatttat ggtgattata gatatgaact ggcaaaattg
    24121 tttcatagtg ttgatggaaa atatgacttt ataattaagg acttgtttac aataaaatat
    24181 gatccaaaga aggcaatcat agactatatt gttcaagatc gtaaacgaga ttatgattta
    24241 tatgaagtat tttactctgt cttcaaagat gaaataggtt ctgatttgaa aaaaattgaa
    24301 cttattgagg ctttgctgtt tttaagtatg attcctttgc atggagaaag cttaaaccat
    24361 cagatggcta tgttagcaac tggtttggaa attttaggac gagttgtccc ggatatttat
    24421 tgttaatgct ttgagctatt gtttttctat ttgatcagtg taaagccggg catataaatt
    24481 gcctggctaa aataaaggag atgtcttagt tatatgttta aagattacgc atttgttttt
    24541 gatattgatg gaactttgtg ccccatcaaa aagaaagatg agaaatatga agatttagtt
    24601 ccgtatgcta atatggtgga gaagttgcga tactataagg agaacggagc aaagattaca
    24661 ctgttcacat ctcgcaatat gaattcttat catggtaatt taggtgtgat caatgcaaat
    24721 actgcaaaaa tcttgttggc ttggctagat aagtgggaga ttccgtatga tgaaattctt
    24781 tatggaaagc cttggccagg ccataaaggg ttttatgttg atgatcgcac agttcgtccg
    24841 gatgaatttt tatcgtgtac cctggatcag ctcgatgaga tttgcaaaaa atccaaattg
    24901 aaatctgagt aaaggagact ttataaagtg aataagatga cggttattat tacaatggct
    24961 ggtcttggca gtcgctttcg taaagttggc tacactgtgc ccaaatttat gattgaagca
    25021 aaaggtaaaa ccttatttga ttggtcctta gacagcttgc ttgattataa ttcacatgta
    25081 gaaaagtatg tttttgttgt gcgtaaggaa gatgatgcta ctgtttttat caaagaacat
    25141 tgcagtctgt atggaattga aaatgtcgag gtagttgaaa ttgactatat gactgatgga
    25201 caagccacta catgtatgct tgcaattcct tattgcgcac ctgataatgc aattatggtt
    25261 tataacatag atacatatgt tgagccaggt gagatgaaat acgttgatat ttctggagaa
    25321 ggacatattc cgtgtttcca tgcggacggt gatcattgga gttttgccaa gttgaacgaa
    25381 tatggtgatg tagtagaagt aagagaaaaa gttagaattt ctaacaattg tactttaggt
    25441 gcatattatt tttcttctgc aaaattatat gcatcactct acgaagaata ttatgcagat
    25501 gaaaggcatc tggaaaagaa tgagaaatat attgcgccgc tatataatta tatgatagag
    25561 aagggcatga aggtaaccat tagtatagta gataaatcaa aagttcacgt actcggtact
    25621 ccagaagagc tcgatgtgtt cttaaatgaa tagggaaaat ataatcatag aaaatgttaa
    25681 ttataaagct gcgtgtgtgg gggagtacta ttcgatagat attctaaaaa tactaatggc
    25741 tattttagtt gttgttcgtc atttaggtca aaactattta gctccagatt aagcatttag
    25801 tattggtatt gttgcaggac tgagtacggt tgccgttcct acttttttca tcatcagttc
    25861 ctttttgttg tgtagtggtg aagttacatc cgctaaatta tctaaacaaa tctacagagt
    25921 acttaaaatt tacattattt ggtctatatt atatattcca ttagtattaa agaattgggt
    25981 acagtcagat tttagctgga aaacagatgt attattgtat atcagagatt ttctgtttat
    26041 tggtagttac tatcacctgt ggttcttgcc tgcactggct tttgctctat ggttagtacg
    26101 caaaagccag gtttctaaaa taaagtttct cagtggtttg ttgctatgcg tagtaggttt
    26161 agcatttgaa tcttattggt ttatattatc tgggaatgat tccatcgagt atattagata
    26221 ttgttataaa agcgtgtttg taacaactcg taatggatta tttataggtg taccatgcgt
    26281 cattattggg caacacttag ccttccacaa aaacactata cttaataagg tctttaaata
    26341 ccgtaatttt atcttatgtg ctgttttagc tataggatgc ttattagttg gtgaaagttt
    26401 catcataaat caaatagctt tgcgagaagt caataatttg ttaatatttt ccattccatt
    26461 agcactggta gttgtatcag tatcactgag cctaaagata gtacatttat aaaaataaca
    26521 gtacatctgc gaagaattag tacgttatta ttttgtttac atccgtatat aatagctgct
    26581 ttaaatttta ttttcccaaa attggggatt acaaattcat taatagaaat gacttgtgga
    26641 atgataataa ttttaatttc tacagttttt gtgtatgcgt gtagtgtgaa atttaccttg
    26701 ctgaaaaaat tatattagat tagtaactgg tacatgtaaa tttgttgaaa ttatagcagc
    26761 atagagatag gaattctgtt gagttttatt ttcgctggcc gaggttattc atgttagagt
    26821 gggtacctcc ttttacgaaa ccttcttgat ggtactcatt tagcgcatga tgaaaggaga
    26881 gtttatgaag aaaatcatat cttctatttt ggttaatgtg ttatctgcat tgtatcaaat
    26941 atttgggttt cgattttaat tgcagttttg gctatgtttt tatacttgtt tgcgatggct
    27001 gaggagaaat caggattagg tttgaaaaaa tcttttcttg tctggattcg gtatttcaga
    27061 aaatcaagct catttagaaa aatgtttttg cttgtgtttt ttacaaatat gattcttttt
    27121 agaacactga tggatagata tgtttggcta aatcctttgt ctaatgtgtt tggtagttgg
    27181 tggatttact ctgtgaatga aacgacaggg gagattagat taactacaga atgttttgaa
    27241 aatgttattc tgtttattcc atttataatt ctagtaatgt ggtataaggc ttcagaaaaa
    27301 gattttggaa aaatttatca gagattataa aatttgtttt tttatgttcg tgtcttatag
    27361 agtttttgca gttattttta cacatagaga cattccaatt gtcagattta ttttataata
    27421 ctttgggtgg cggcattagt ggggttatat attatttgag ctgatatatt catcctaacc
    27481 ttaaaaataa atgagcagaa ttttaaaaga ttggagagat tctatggatc ataatgcaaa
    27541 ttattatcat tggcttacaa atgccaatgc agaaattgta gaggaactgc ggtcatattc
    27601 cgagaaggac attgaggact ccttctataa aaatctgtcc tttggtaccg gtggtctgcg
    27661 tggcaccatt ggtgcaggca ctaatcgtat gaacgtacac accgtaggta aggcaagcca
    27721 gggactgtca gactatctga ataagaccta tgaaacccct tctgtcgtcg tgggctatga
    27781 ttcccgaatc aagtccgatg tatttgcaaa ggttgccgcg gacatctttg ctgcaaacgg
    27841 tattcaggtg catctgtggc cggagctgct gcctgtgcct accgtatcct ttgctatccg
    27901 ttatctgaag gcttctgccg gtgttatgat caccgcatcc cataatcctt ccaagtataa
    27961 cggatataag gtctatggcg caggcggctg ccagatcacc accgaggctg ccgctgagat
    28021 tctggctgaa attgaaaagc tggatatttt tgcagatgtg aaaatgggcg gcgacgcttc
    28081 cagaatcgct tctatccagc cagaggttct gactgctttc attgaagaag taaagggcca
    28141 gtcggttctg tttggcgaag aagtggacaa gaatgttgct atcgtgtatt ctcctttgaa
    28201 tggtaccggt ctggtgcctg ttacccgtac tctggcagaa atgggctaca gcaatgtgac
    28261 cgttgtcgaa gagcagcgtc tgccagacgg taatttcccg acctgtcctt atccgaatcc
    28321 ggaaattaat gaagctatgt ctctgggcat ggagtatgcc aagaagtata atgctgatct
    28381 gctgctggct accgaccctg actgcgaccg tgtgggtatc gccgtgaaga atgcagaagg
    28441 ggattatgtc ctgctgtccg gtaacgaaac tggtctgttg cttctggact acatatgctc
    28501 tcagagaatt aagcacggca agatgcccac tgaccctgtg atggtcaaga ccatcgtgac
    28561 tatggatctg ggcgagcgga tcgcttccaa ctatggcgtg cgtactgtcg atgtgttgac
    28621 cggcttcaag ttcatcggcg aacagattgg tatgctggaa aaggcaggca aggtggacag
    28681 ctatattttc ggctttgagg aatcctacgg ctatctatcc ggcagctatg tccgtgacaa
    28741 ggatggtgtc aacgccgctt atctgatctg cgagatgttc tcttactaca agactcaggg
    28801 tatcagtctg tttgataagc tgaacgagat ttatgctaag tatggctact gtttgaacac
    28861 cctgcattcc tttgagtttg agggtgcggc aggttttacc aagatgcaga aaattatggt
    28921 tgaattccat aaaggtcttg atatgattgg aggtaagaag gtcattgaaa ccttggatta
    28981 cagcacggga ctgaatggcc tgccgaagtc tgatgttctc aaatatctgc tggagggcaa
    29041 ctgctctgta gtggttcggc cttccggcac tgagccgaag ctgaagacct atatcagcgt
    29101 tagcgcagaa agcaaggaag ctgccgaagt aatggaagct gttatggctg ctgaactggc
    29161 tgaaatgttt aagtaatact atgaaaaaga tctgagtgtc aatgtccgaa gtagcaacat
    29221 ctatgtttaa taaaaatttc ctgctttacg gttctgttta cataattgag gtaaaggggc
    29281 cgactgtgtg gatcgaaaag gcaaagaagt gttaacagag acacagcagc ctgatgaagc
    29341 accgatcaaa gcagtaaggg agaaataaga gatattagag ttttaataat gtattcagca
    29401 atagagatga aaggagtaga atatgaataa aactattgaa ataataaaat gagggggaaa
    29461 gaaataaacg tgatattatt agttttgaga aggcgttttc aatattcgct attgtattga
    29521 tgtactattt tcagcaatcg tatttgccag caatactgaa caaagcaata tctattggtg
    29581 atacaggagt gcatatattc ttcttttgct caggttttgg gctatatctg tcatatttaa
    29641 aaaagcctat tacattgggt gaatttttac agaaacgatt tatcaagatt tatattccgt
    29701 atattatcgt aattacggta tcagtgttac taccttatat gacacaagat ttaaatgctg
    29761 caaaagtata tttgtctcat gttttgttat ttaaaatttt tctaccagta tatgaggagt
    29821 cactgggtcc ctttggttta tttcgacaat tattcagttc tatttcttat tcataccaat
    29881 tgtaaaacta tagagaaaaa taggaatgaa atgattgctt gttgcaagct gagttattag
    29941 tatctgggct attatgatgt ttcatacagg atatggaagc atacgagttc ttgggagctg
    30001 ctttgcgcaa tatatttggg aatttacttt cggcatgttt ggttgcagag tatttagtga
    30061 aaagaaaaat agtaagattt aataatatat tcgtgattat ggcgtccatt gttggaattg
    30121 gttttgctgg tatatcgaaa attattggtg gactgatgga gtctattaat gatatttttg
    30181 cattcttcgg ttacggtgca attgcaattt taatgtatca agttattcca tttagaaaga
    30241 taatgcctag gatttcaaga tactcatatg atatatatct actccatatt cttgtattta
    30301 gtacgattca tcaaattatc cctaacttgg ttttggcggc atttgtttcc attcctataa
    30361 cgttggtgtt gtcatattat tatagtatta tgttatatag acttaaatta aaatgatagc
    30421 ataattgtcg cctctaccgg gcggttgcca tcctgattaa cagcctccac gcggcggatg
    30481 gtgggcactt aggtagttcg taacctagtg gtagatggtt ggtaaaaagg agataaaact
    30541 aaatgattac acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
    30601 ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa gccagtaata tgctttcaaa
    30661 tctactctct agccctttaa cagaacgtga gttgaaattt tgacaaccta atttttct
//
LOCUS       DAPBAB010000029        28991 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPBAB010000029 DAPBAB010000000
VERSION     DAPBAB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.029"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..421)
                     /locus_tag="RD444_001585"
     CDS             complement(<1..421)
                     /locus_tag="RD444_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDT7548962.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKEN"
     gene            581..1087
                     /locus_tag="RD444_001586"
     CDS             581..1087
                     /locus_tag="RD444_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7548963.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1255..3168
                     /locus_tag="RD444_001587"
     CDS             1255..3168
                     /locus_tag="RD444_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDT7548964.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3173..4174
                     /locus_tag="RD444_001588"
     CDS             3173..4174
                     /locus_tag="RD444_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7548965.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4376..4582)
                     /locus_tag="RD444_001589"
     CDS             complement(4376..4582)
                     /locus_tag="RD444_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDT7548966.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5228..6076)
                     /locus_tag="RD444_001590"
     CDS             complement(5228..6076)
                     /locus_tag="RD444_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7548967.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            6075..7142
                     /gene="gnd"
                     /locus_tag="RD444_001591"
     CDS             6075..7142
                     /gene="gnd"
                     /locus_tag="RD444_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDT7548968.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            7144..8145
                     /locus_tag="RD444_001592"
     CDS             7144..8145
                     /locus_tag="RD444_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDT7548969.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            8142..8426
                     /locus_tag="RD444_001593"
     CDS             8142..8426
                     /locus_tag="RD444_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDT7548970.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            8459..9256
                     /locus_tag="RD444_001594"
     CDS             8459..9256
                     /locus_tag="RD444_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7548971.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            9270..10103
                     /locus_tag="RD444_001595"
     CDS             9270..10103
                     /locus_tag="RD444_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7548972.1"
                     /translation="MEILSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            10121..10978
                     /locus_tag="RD444_001596"
     CDS             10121..10978
                     /locus_tag="RD444_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7548973.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            11000..11509
                     /locus_tag="RD444_001597"
     CDS             11000..11509
                     /locus_tag="RD444_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7548974.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            11546..11947
                     /locus_tag="RD444_001598"
     CDS             11546..11947
                     /locus_tag="RD444_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548975.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            12059..12412
                     /locus_tag="RD444_001599"
     CDS             12059..12412
                     /locus_tag="RD444_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548976.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            12446..12889
                     /locus_tag="RD444_001600"
     CDS             12446..12889
                     /locus_tag="RD444_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7548977.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            12906..13991
                     /gene="uxuA"
                     /locus_tag="RD444_001601"
     CDS             12906..13991
                     /gene="uxuA"
                     /locus_tag="RD444_001601"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDT7548978.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            13984..14697
                     /locus_tag="RD444_001602"
     CDS             13984..14697
                     /locus_tag="RD444_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7548979.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            15026..16327
                     /locus_tag="RD444_001603"
     CDS             15026..16327
                     /locus_tag="RD444_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7548980.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(16405..16554)
                     /locus_tag="RD444_001604"
     CDS             complement(16405..16554)
                     /locus_tag="RD444_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548981.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            16950..17150
                     /locus_tag="RD444_001605"
     CDS             16950..17150
                     /locus_tag="RD444_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7548982.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            17718..18281
                     /locus_tag="RD444_001606"
     CDS             17718..18281
                     /locus_tag="RD444_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548983.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(18342..18707)
                     /locus_tag="RD444_001607"
     CDS             complement(18342..18707)
                     /locus_tag="RD444_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548984.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(18783..19028)
                     /locus_tag="RD444_001608"
     CDS             complement(18783..19028)
                     /locus_tag="RD444_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7548985.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(19098..19352)
                     /locus_tag="RD444_001609"
     CDS             complement(19098..19352)
                     /locus_tag="RD444_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548986.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(19516..19839)
                     /locus_tag="RD444_001610"
     CDS             complement(19516..19839)
                     /locus_tag="RD444_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548987.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            20026..20099
                     /locus_tag="RD444_001611"
     tRNA            20026..20099
                     /locus_tag="RD444_001611"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20060..20062,aa:Ile,seq:cat)
     gene            complement(20484..20981)
                     /locus_tag="RD444_001612"
     CDS             complement(20484..20981)
                     /locus_tag="RD444_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7548988.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(21021..21830)
                     /locus_tag="RD444_001613"
     CDS             complement(21021..21830)
                     /locus_tag="RD444_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7548989.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            22164..23021
                     /locus_tag="RD444_001614"
     CDS             22164..23021
                     /locus_tag="RD444_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDT7548990.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            23014..23916
                     /locus_tag="RD444_001615"
     CDS             23014..23916
                     /locus_tag="RD444_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDT7548991.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            23949..24299
                     /locus_tag="RD444_001616"
     CDS             23949..24299
                     /locus_tag="RD444_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7548992.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            24348..25271
                     /locus_tag="RD444_001617"
     CDS             24348..25271
                     /locus_tag="RD444_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7548993.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            25349..26242
                     /locus_tag="RD444_001618"
     CDS             25349..26242
                     /locus_tag="RD444_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7548994.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            26280..26843
                     /gene="amaP"
                     /locus_tag="RD444_001619"
     CDS             26280..26843
                     /gene="amaP"
                     /locus_tag="RD444_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDT7548995.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            26857..27045
                     /locus_tag="RD444_001620"
     CDS             26857..27045
                     /locus_tag="RD444_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDT7548996.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            27058..27960
                     /locus_tag="RD444_001621"
     CDS             27058..27960
                     /locus_tag="RD444_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7548997.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            27992..28234
                     /locus_tag="RD444_001622"
     CDS             27992..28234
                     /locus_tag="RD444_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7548998.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(28490..28861)
                     /locus_tag="RD444_001623"
     CDS             complement(28490..28861)
                     /locus_tag="RD444_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7548999.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        10096 a         4747 c         5824 g         8324 t
ORIGIN      
        1 tattttcttt aaaatcgaca agatgaccag aaagcaagag ttcgttgagc tcttgctttc
       61 ttttagtttt agaaaggaac atcctgattt tatcttcgct aaatccagtc aaaagttttg
      121 cagattggtc gtagataatc ggttttttca ataaagaagg ggatgtacga atctcaatat
      181 atagttcact caaactgagt tcatctattc tgtgaacaag atttttataa ctaatagaat
      241 gagtagagag catttcggtc acgccgttgt ttgtcaattt tagtaagtgg ataaattcat
      301 ttttggttag tggttgacga attaagttct gttcgataaa cggtatatca tggacgataa
      361 gccaacgttt tgcgcgtctt gaagacgagc agccagaacc tgtatacaat ttaatcatca
      421 tacattcctc caatagctaa gtatttatga aataataaca tgaagaaata gtcgaaaagt
      481 gccatttaga ggtgtaaaat aagaaaatgt tgtcacgtat aatggaacta gctagataag
      541 aaatactatt aaatcaagaa aaaagaaaga aggcacaagt atgtcaaaca cagccgacgc
      601 aatagtagtg atcgatttac aaaaaggggt atgttttgat tcttcgccga tacacgaatt
      661 agaaacagtg acagcattag tgaataaaag aatcaaaaca tacgaaacga ttgggaaacc
      721 agttattttc gttcaacacg aagatgctca attagtgcca caaacaaaac catgggagct
      781 attgtcggaa ttatctattc ctgagcacag ttattttgtg agaaaaaccc atgcgaattc
      841 attttataag actaatttga gtgttttatt aactgaattg gactgtcatt cgatcgaatt
      901 atgtggggca caagcagagt attgtataga tacgactgtg aagtttgctc atggattagg
      961 ataccagctt atgatgtgta gaggagcaac gacaacttat gataatcctt ttatgaaagc
     1021 aaaggaaaca atcgcttttt acgagaaaat ttgggataaa cgatttctga catttttgga
     1081 gcaataagtt gtttcttcgt ttaaaaaaag cagaatggat actggtttat ccattctgct
     1141 ttattacgta aaatgagaaa ataatcctgt tctattttta tgggataaac catttatttt
     1201 ttgtataaaa aatgtttgcg tttacatata attctcctag aaacataaaa aggagtgaat
     1261 agcttgagaa caaaacaagt attaaaggga gcgttggtca tcttatttgc tgtaagtaca
     1321 agcgtcccgc aatggacagt atatggagag agcatttctt ccgaatcgga atctcgggag
     1381 gtagaaacca cagaaactac tgatgctgga aatgaaacga aatcgagtac ttcacaaaca
     1441 gaaacaaata ctgaaacaac ggttgagaca tctgttacta gcacagatca agaaacgacc
     1501 gctaccacag ataaccaaca aacagaagaa gcaacagcta caaaaatgtc aaacgacgaa
     1561 tcctcttctg ctaaagtaag tgacaaagag aatgggacag aaaatgatga tatcggtaaa
     1621 ggtgaaaatt tcaatggtaa agaatatttg aattacttcg gtaaacagat gacagaaata
     1681 cttaatggac caacagaaga catcaagtca aaattaatag agttattagt cactgagctt
     1741 gctgttaatg attatggtgt agataaagag attatcaaac atctgattgc tacttatcta
     1801 ttgtacgata tcgcttttgc aaaaaacagt gtgttgatgt cgatgcttgg aggtacagat
     1861 gatgggacaa cgcctacaga aaacacaggc ttctatccaa atacaaaaca acttcatcaa
     1921 actgtactaa acaaacaaga tggcaaaatg atggatgtat atgcatatta tgtcgatcaa
     1981 ggttcagata aaacagtcat gatccatggc ggatttcgag ggaattggaa taacgggatc
     2041 gttacagaag aatacaatga tttttataag gccgggtata atttattatt tgttgattcc
     2101 agagcgactg gtaatagcgg aggcgattat gtgacctatg gacaatatga gtcagatgac
     2161 gtcctttact ggataaatca agaagttcga gagcggccat ctcaaaagat tttactttat
     2221 ggtggttcga tgggagcagc tacgatgatg tctgtattag cgaaagatat tcctgtcaat
     2281 gtcaaaggaa ttattgaaaa ttgcggattt gcatcaattg atgaacaact acgttttacg
     2341 tacagccaga cagtagcccc cgcactaccg gatgccataa aaaatcagtt agacatcatt
     2401 ggggatcaag agcatgaaga tttatttatg ggccttttga aacaatacta ttttgatcag
     2461 gagatgcatt tggatacaaa agctgctcta cctacgattg gaatgtcaga ttcattgcct
     2521 aaattgatta tccatggaac agcagatgat gtagtacctg tgtctaacgc gcaaaaatta
     2581 tatgagttat caggagggta taaagatctt ttattagtag aaggagcagg gcatggaaag
     2641 gcacaggaag ttgaccatgc tgcctacacg aaacatgtaa cggacttttt gaaagtagtg
     2701 ttccatgatc agataaacgt aaagtatgtg gatgaaaaca ataaaagtct actcaaagac
     2761 caggacgaaa tccagcttta cggagcttat ggagaaaatt atatgacaga gcaaaagaca
     2821 ttcgaaggat acgaattagc aaatgttgaa ggaccgactg aagggatttt taatgagacg
     2881 ataccaacta ttattttcta ttacaaaaaa atcccagtag ctccaccaaa aaagcaagat
     2941 ccgacagaga atgcagacaa taagggaaag aatgaacctc gagaggatca atcaagtgat
     3001 gatacgcaac cgattcagat gacagtgaaa gtacaaaaaa agacagatgc aaaaaaaaga
     3061 atgttgccga aaactggaga aaagaaacat tcgcttctca tttattcagg tggattttta
     3121 tctgttagca cgattatcac atggatttgg cgaagaaaga gagggtaaat gaatgaaatg
     3181 gttcaagcga atagtgattg cagtaccagt tatcatcgta gttattttag tcactgtatt
     3241 tttgattaat caaatcacac caaagccgat tagcttgctt gttagaaaac aatttgacac
     3301 aagtgaaaaa gaacaagtat atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat
     3361 cgatataaaa aaagaaatat cctatccttc aaagtacaaa aataataata tggatatcta
     3421 cacaccgaaa gtggaaaaga aaaaattacc gattcttttc tggatgcatg gaggcgcata
     3481 tgtaggaggg gataaaaatg attgtcgtga ttatttggaa tatctatgtt cagacacgca
     3541 gcaaatcatc gtaaatatag attatgaacg atcaccggaa gcaaaacatc ctacaccagt
     3601 tatccaattg aacgaagcca ttcaagcagc taaaaaagag ataaaagatc aagcagattg
     3661 gtcgaatatt tcgattggtg gaaattcggc aggtgcacaa attagtggag aatatttgtt
     3721 agcacttcaa aatgaagaaa ttaaaaaagc tgatcatctt gatccgactt tggagaacaa
     3781 gcagattacg aagtttattt ctttgtctgg tctgttggat ccttctgcac ttactcaagt
     3841 aagtgataaa atctctagct ttctttatga aaaatgtgga tgggcttact ttgataataa
     3901 ggactttgaa caatcagaga aggtcaaaag tttagccttg gtcagacatg cggatcgttg
     3961 gacgcaaaac acattcttaa cggatggaaa tacaaatacg tttacgaaac aaatggaaga
     4021 agtaacttct gtatttgaga aagcgggggt gaaagtcaca aaagtagatt atgcggagaa
     4081 aaatgctgtt ttgaaccatg aatatcagtt tgatttcagc aataaacaag cgcaagaaac
     4141 atatcaaaag ttagtaatgt ttttcaagga gtaaagtctt atgatgcaaa agaggttgta
     4201 atctgtcact tagctccaag tcacaaggag caatttcaat aaacaagctt ttcatggttt
     4261 atgaatattg taacttgtga ctgaggagtt agagatggca aggggcacct tttattcgaa
     4321 atttgggaat atcacacgat tattgtcacg atctctttta cgtaaatttt ataagtcaga
     4381 tttgttgttt tgatcgttta aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg
     4441 acaaaaagcc gagtggtaag caaaagaatg ctggttccac taatattccg ttgttattga
     4501 tataaggctg ggaacaaaga tgaatagcaa ataaaatcaa tgcactagcg aaaaaaagca
     4561 aagaaatatg gatagctttc ataagcagca cctccttttt tataattgta tgataaatag
     4621 attaagaatc tataaatgtg acaagtttgt taggatgtga ataaaatgat catctagcga
     4681 acttgaaaca aagatggcta atgataagca attaatgatt cagcaatcag ataggtacaa
     4741 agtttaccat catacgtcta gaccaataat agaaaatatg ggaatataat agaaaagatt
     4801 taggataccc ccaaaagaat ttccccaaaa attcgatgta tcctaaactt ttttatttta
     4861 cataacaatc atatcaactg tgaaataaaa ttacaaatga taacctaaaa tgaggccaaa
     4921 agccagtgtt atcaaggaaa tacttaattc accaaaattt tttttaattt tttagaaaat
     4981 aagaaacaaa aataataaaa aaaagaaaag ataaattttt tactactgta tactaacttt
     5041 gttatcgcat ctaacaagta actcgaataa taccaataca accttctcca aagatgcgat
     5101 gacatacgtt ctaacccacc gactattgat gttctgtacc acccttgcag aaccaaaaaa
     5161 gccactacca aatatttggt agtggctttt tttgttttat ggatttttcg ctttttctgt
     5221 tatggtgcta ttctttgatt tcagaaatag tatgtcgaac tttagcgata gaacgacgtg
     5281 attctttttc gttgtaaaac atcaaaatca catatagaga gtccaaaatg cttagttgag
     5341 aaatacggga agctaaagct tcagatcgga attctgtttc ttcagaaata gaaataaaaa
     5401 cgacatcgcc aagtttagca agggaagaat tagcttggct agtaataacg attgtttttg
     5461 ctccagtttt tcttacggtt tcagcaatat ggattgtttc ttttgattgc cctgtgtgag
     5521 aaatcaaaac agcacagtct ttttccgtca ataaagaagc ttccatcaat tgaatatggt
     5581 aatccgtgct gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa gcgtcagttg
     5641 ctacaatctc tgaaccgcca acaccgaaaa aatagacacg tttagattca ttgataatag
     5701 tagccgcttt ttttagatcc ttttgttgta acaacttttt tgtgtcattt aaagttgaaa
     5761 tactagactc gaacaccttt tgcgccatag tgagctcagt atctgttttc tgaatatttt
     5821 cgtggataga aatagcagat agatcatttt cttggatcaa catggccatt ttaaaatctt
     5881 taaatccatt ataaccaagt ttttttgtaa attgaaaaaa agtagagtca gcaatgccca
     5941 gttcacttgc taaattgcta ataggactat gacttacttt gcttggattt tctaaaatat
     6001 aatcggcgat agcctgttct tttgggctga agtcgttgta cattgttttg atttgtagtc
     6061 ttatggagac gctcatgaga attcctcatt ttcataataa gattatcatt ttttccgttc
     6121 tcattaatgt taaagataca aggagagtag gaaaaaatca aacaaaaaat atttttgtaa
     6181 aatctattta caaaaaaatt tttttgtata atgagggtat aagaaatgga ggcgctatca
     6241 tggaaattgg tgtaattggt ttgggaaaaa tgggattgaa tttagcttta aacttaaaag
     6301 agcaccatca caaagtacaa ggactcgaca tttcaagtac agctgttagt caggcaagag
     6361 aaaatgacat tcctgcttat caagagatct ctgaatttct agctactttt gagcagaaaa
     6421 aaatcatctg gttaatgctt cctgctggag agataaccga aaacatgtta agaaaacttt
     6481 ttccaaaact cggaccagga gacattatta ttgaaggcgg aaactcttat tataaagatt
     6541 caatccgtcg agcagaagag tttcagaaaa atgaaatcgg ttacttggat tgtgggactt
     6601 ctggaggaat cgaaggagca cgacatcaag cctgcttaat gattggagga gataaagaaa
     6661 cttttctatc cgtggaacaa ttattcaaag atgtagcaat tgaaagagga tatctgtatg
     6721 ctggtgcatc cggaagcgga cacttcttga aaatgatcca taatggaatc gaatatggaa
     6781 tgatgcaagc aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat gcttttgact
     6841 tggaacaagt cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg ctgatggaaa
     6901 tcatggaaga tcagttaaac gaacatccca atttaagtaa ttatcgggga atcgtttctg
     6961 catcaggtga agcaaaatgg acggttgaaa cagcgctaga aatggctgtt ccagttccag
     7021 taattgcaac tagtttgttc atgaggaatc tttcacagga agaagatagt ttttcagcaa
     7081 aagtagtttc agcactaaga aatgggtttg gcggacatga aatagttgaa aagggtgagt
     7141 aaaatgccag attatcatat agcaatcgtt aattccagca gtttcggaag agtctttcca
     7201 caccacttgg aaagattaaa gtcagttgga gacgttcatt tctttactgt tgatcaagag
     7261 attccaggaa aagaactagc ggagttactt aaaggattca atatcattat tgcaagtgtt
     7321 acgccatttt ttcgacgaga tttttttgaa aacaaagatg aactgctatt gatttcccga
     7381 catggtattg gttacaacaa tattgattta aaagcagcaa aagaacacca gacaatcgta
     7441 tctatcatac ctgctttagt tgaacgaaat gctgtagcag aaaataatat aactaatttg
     7501 ttgtccatgc ttcgacgcac gacacaagca caagcaagag tcaaggacga tcgatgggaa
     7561 gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt gattggtgca
     7621 ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta
     7681 gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa aaaagtagaa
     7741 cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt gacagaagag
     7801 aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt ttatctatct
     7861 aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct gaaaagcgga
     7921 aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca agatcatccg
     7981 tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac aagggaatgt
     8041 ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca gcaccttctt
     8101 cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag aaaatggtga
     8161 acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt ggtatttcaa
     8221 aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc cacggaacgg
     8281 tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg attcaatttt
     8341 tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata gctacaatca
     8401 tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga ccgatctagt
     8461 gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt tagctagtaa
     8521 ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag cagaagggaa
     8581 aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat tagtgattga
     8641 ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat ggtatgaatc
     8701 tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc tagcacaaga
     8761 tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa tccaagatat
     8821 ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct ttgctgttgc
     8881 tggtttagtt ggaggattag gagataaata cgcaagaaat gcggcagcac atgctatgca
     8941 tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag aaaaagtagc
     9001 gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag acgagttata
     9061 tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat tatttccgaa
     9121 agataaacag ataatagata aaatggctga atttattgat tcgaaagaga aagtgcattt
     9181 gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac tggaaggtta
     9241 tatcagaaag atctgaagga ggaggacaca tggaaatatt aagtgtagta caaagtttgt
     9301 tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga gccacgttga
     9361 ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg ggggaagttg
     9421 cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt ttttctccag
     9481 gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca gtcgcattat
     9541 taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa
     9601 gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat acagacaaag
     9661 ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat ccaacggttg
     9721 cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg gcgccattga
     9781 tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt gaagtagtag
     9841 gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt cgaaaagatt
     9901 atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct ttagatatta
     9961 cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa
    10021 aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt gatgacgaag
    10081 atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa taatggataa
    10141 tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct atctgagatg
    10201 gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat cactactata
    10261 cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa aagcagcata
    10321 taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg tgattactgg
    10381 cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa aagagatctt
    10441 gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac tagcaggaat
    10501 cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg cggtaccttg
    10561 ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg ggttgtatca
    10621 ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta atgctacagg
    10681 cctaatgcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt tagggttgtt
    10741 tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga aatttgcgat
    10801 atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag ggatattacc
    10861 tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga aagtcacaca
    10921 agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta ttttataaaa
    10981 agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg agcgtttgat
    11041 ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca tttttgttgt
    11101 agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg caggaagccg
    11161 tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt gggatgaata
    11221 tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga tttacgactt
    11281 agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc aaaaaaatcc
    11341 tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt
    11401 gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca ctccatctgc
    11461 acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa caaatgctta
    11521 gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt
    11581 tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc gttttctttc
    11641 agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg cttacgatac
    11701 gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag agaaagttat
    11761 ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt ttgattggaa
    11821 agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt tgttcagttt
    11881 aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg atctgctatc
    11941 cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat tgggaaaagc
    12001 agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca tattaacgat
    12061 gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg
    12121 ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag aggctattct
    12181 tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa attatttttc
    12241 aaataaagaa cagtggctga aacaagttga taagttgaaa gaaaaaaacg tcaattatat
    12301 ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag atttacgagg
    12361 gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat aagtaaaaag
    12421 tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac atggtaagtt
    12481 gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc atattgaaac
    12541 aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca aagaaaaatt
    12601 aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta gcgcaagtcc
    12661 gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag cagccactta
    12721 tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc agatgcttca
    12781 cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata atttaactgt
    12841 ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga aggaaggctg
    12901 aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc ccattaaaat
    12961 atgttcgcca aataccggga ataaatggga taattggaac tttgctgact aaaatgcctg
    13021 gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag agccaaggac
    13081 tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca gggacagatg
    13141 aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct cgatgtgggg
    13201 ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact gatttgttta
    13261 atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc gatcagatgg
    13321 agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt
    13381 gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa ggaatgaccg
    13441 aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa
    13501 aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt tttggttttc
    13561 ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta gattctccat
    13621 ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac gatttagttg
    13681 aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac gtggagtttt
    13741 taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc cttgatatgt
    13801 atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg
    13861 gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg
    13921 gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca ctgggagagc
    13981 attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt agaaactgga
    14041 cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga aatgcgtaag
    14101 gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca aatgggcatt
    14161 ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca atataaagaa
    14221 aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga aattttatct
    14281 tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta
    14341 gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat ttcaggggat
    14401 gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc aaaaatgaat
    14461 attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga agcagcaagg
    14521 cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc cgatcataat
    14581 gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc agactatgaa
    14641 ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta tctctaataa
    14701 agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg aacagatgaa
    14761 aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa tttagaacaa
    14821 acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag aacgagaatc
    14881 gtccatatga gggtttccac ggctctataa tttctaggac tgatagaaaa tcattctatc
    14941 ggtccctttt gagcatgaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca
    15001 caacacaatc aaaggagcgt tctgtatgcc tgatcttcat catatcaaac tactacttgg
    15061 aatcaaagat aaaaacattt tcatcacaaa tgtggaatcg aagagcatta agaatatcaa
    15121 gagtcttgtt gtttctgcga cgttaagtaa agaaattcgt cgctgtcctt tgtgtaaaca
    15181 gatgaatcat gaaggcatga tcgttaagaa cggaaagaaa aaatcgctca ttcaacttaa
    15241 taaatgtgcg aaccaactga cttatcttgc tttagcgaaa caacgctacc actgtcgtgg
    15301 ctgccacacc tattttacgg cgaataccta cattgtcgat agaaactgct ttatcgctaa
    15361 acaagtgcgc tataaaattt tagaagagct aactgaaaaa caagcaatga cgacaattgc
    15421 taagcattgc ggcgtttctt ggtcgaccgt ttctcggaca ctcgcttctc tgatccccat
    15481 gacaaaagta aaaagaaatt ggttaccgcg ttgtttatta atggatgaat ttcgttcact
    15541 gaaaaatcaa gtcggtccct actccttttc ttgtatggat ggcgatactg gaaaattact
    15601 ggatattctt ccttcacgga agaaaaaggc tctcgtttct tattttatgc aatttgaacg
    15661 acgcgcacga ttgaaggtaa aggtcatcgt gacggacatg aatgtttctt atgcctctct
    15721 gataaaggaa tgttttccaa aggcaaaact cgttgtggat cgttttcaca ttgtcaaaca
    15781 tctcattcgt aaatttgagg atattcgcgt acgaatcatg aaaagctttg atcgaaacga
    15841 tccaacacaa gccaaatatt atcgacaatt aaaggcgttg tctcggcttc tgatcaaacg
    15901 acaggacaag cttgtttatg acaaatggac caaatggcgc aattttggtt gggcgtatct
    15961 caccgaaagt gaagtggtgg atcgtctgct ttcgatctct gacgaattac gaatcgccta
    16021 tttttactat caagaaattc ttcaagcgtt tcacgacaaa gaggcggaca ctttctttaa
    16081 acttgtaaga acgatgccca attccgttcc aaaagaactg cattctataa aaaaagcttt
    16141 tataaactat gaatccggca ttcgtttagc cctcgaattg ccttattcaa atgccaaaat
    16201 agaaaatctg catacccaca tcaaagcatt gaaacgagtc gcttatggct ttcgaagctt
    16261 tcggaaaatg aagaccagaa ttttcttact aaataatctg attacctatg aatcaaaaaa
    16321 catctaaagt agaaaaggaa ttctacctta gatgttctta tctcactcta tcagtcctat
    16381 ttgacaaaga gccgtttcca ctccttatgc agattttttt aattcttgca ctgcttgttc
    16441 gatcgtaaca cctgtcgcta catgattggt attttctttg tcaaaaacga caaatagatt
    16501 caaatctgta tccaaggtta ctcgatattg tatatctttg ttatagaatg tcatagccat
    16561 tctcctttct ttaaatactt gctgaaaggc aacgaacgcg ttgataacga aattccattt
    16621 ataagtatac caccaattga aaggttttca tagttaagaa attttaaagt caaaagttgc
    16681 agataaaagc aaataaacaa gaaaacaaaa agtgaatcaa tcgcggttat tcaacttttt
    16741 cttcgattcg aagacaatag aagacaatga attttatatt tcattgcatt atccttgtgt
    16801 tattagaaaa agcatgatat attaaatgag taatctattt tgaaacgtaa tttgagcgat
    16861 atatctacat agataaaact ccttttgcct atcaaatatt tattgcaacg aaaaaacgta
    16921 cattgatacg tatcaggagg aatttattta tgaataacgg tacagtaaaa tggttcaacg
    16981 cagacaaagg ttttggcttt atcactggtg aagatggaaa tgatgtattt gcacattttt
    17041 cagctatcca aggtgacggc ttcaaaactt tagaagaagg ccaagcagtg tcttacgatg
    17101 ttgaagatgg tcaacgtggc cctcaagcaa caaacattgt taaagcttaa taaaaattga
    17161 cttttaaccg tcttttcgaa agaaaagacg gtttttttgt acgaaaaaat tcattggtcg
    17221 ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc ttttatggta
    17281 gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat atgaatcgag
    17341 atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta cagtttcgtt
    17401 cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa gagggcggca
    17461 catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag gctatcctac
    17521 tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt cattaaatcc
    17581 cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatctgt ttttttaata
    17641 acgtaataat ttttttattt tttgtgcttt cgccgttatt gtagagtgat ttagcattct
    17701 tatttttaaa aataactgtg ttatatatta aaaaaggcat aatagttata agctttttta
    17761 aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc gttattattt
    17821 tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta tcaatattaa
    17881 cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc acaaaatcaa
    17941 ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac agaggcttaa
    18001 atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa
    18061 gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc aatctgtgct
    18121 gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg agatgttcat
    18181 ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt tttgcacgtg
    18241 aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg gttaaagctt
    18301 tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag ataaattcct
    18361 ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc cggataaatc
    18421 ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt gccacgatac
    18481 ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc atccatgaca
    18541 agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc cacagtagca
    18601 tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg tcctaaaagc
    18661 tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga aatgtctcct
    18721 ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa
    18781 aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg
    18841 taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc ttcttcgtac
    18901 ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg agataaagat
    18961 cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg
    19021 atactcattt aatcaccttt cattatagta gaatagttat aatgactata gaaagctaca
    19081 catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag aatgacgatc
    19141 aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg ttcaaagtag
    19201 ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat agctaaataa
    19261 aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc gaccgcaggc
    19321 acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg attcatataa
    19381 taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat aaaagaagaa
    19441 tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg aactgttccg
    19501 ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt aattcaatac
    19561 atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc catagcgaat
    19621 ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc ctttatagct
    19681 gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc caataaacag
    19741 aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa ctaaatcgaa
    19801 taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat aaaagaataa
    19861 cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg acacaagtaa
    19921 aagaaataag aataacaata ttattttact tatattttga aacttgtgag agtttgtcat
    19981 tcgtgctata gttgaaaagt aacaaaaata aattttattt atgatgtacc tttagctcag
    20041 ttggttagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta caaggtacat
    20101 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct
    20161 tcttatgttt ttacttggct gttcttatta ctgcagatgc aagcttttga ataccgttta
    20221 aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa cggaaaaata
    20281 gttcctcata ttttgccgtt tgttaagcta ataccgaggg tcagctgatt gaacaccatt
    20341 taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc aatctacttt
    20401 gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct ttttttattg tgtgttaaat
    20461 atcgtaaatg aagatagata tttttatgat tcgacgatag agacgttgaa atctttcaac
    20521 agctctaaag cggattctcc gtgttttcta ctggagacta acattggatc aacaaaaaag
    20581 tctgcttcag gaaaagcact ctgcaggcct atcagattag agagtacaca caagtttgtt
    20641 tcaatcccga ctacttcgat ctctttttgt tcttctttgc tttcaaataa agtttcctgg
    20701 atttcagcta aagtttcagg tggaatcgta aagtaatact ttttaatcac tcgttcattt
    20761 tcttgtggag aaagaagagg gatcaattgc aaatcttctc gttcttcttc tttgtccttc
    20821 agttcaactg gtatatcccg tgtgaagaga atatattcat tattttggcg tgctttagct
    20881 aatctttgag caatacgttc tgccaattga tctgactctg gaatataaaa ttcgctgttt
    20941 ggatcaagaa tatgattttg catatcaata ataactaaca ttttctttcc tcctacattc
    21001 atatcgtcat tttattcttt ttaatagaag ttttttaaaa tataagcaac accagcttct
    21061 tcattcgtca gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc attccccatc
    21121 gcaatactca acccgctgta attcaacata gcaatgtcgt tttcttgatc gccaaaagcc
    21181 atgatgtcac tcgattcgat tccaaggtaa tccgccagaa aaacaagtga atgagcttta
    21241 gtgatacctg taggcgtgat ttctaagaaa tgcggatcag agaatactga ttggatacca
    21301 tctgcttgca gatctctttg tagcttctgt agtttttctt cactcttatc tgttaatgct
    21361 aatttaagat agctaagaga aggatcttcc aacatattta aaatttgatc aaaagaatgg
    21421 ttctgtaaat tgaaatgttc ataataaggg tcatctttga ctagatttga ttcatcgaat
    21481 gtcagctgat catcttgggt ttctcggcta gcaatgatgg atattttcgc ttcttgagca
    21541 tagaagaagg cttttctcag tgtatcgcta tctatttttg aacgtctgag aactttcgcc
    21601 atttttccat ccttcatttc agcaatcagt gaaccattgg aagaaatcaa atagccaccg
    21661 attcctaatt cggaaaaaat atctttagca gaaatgctgt tccttccggt agcgacggtt
    21721 acgattccgc cattttcatc aaatttccga agtgcttgga tattttcttc tggtatcttt
    21781 ccatttcgat caagcaaggt accgtctaaa tcaatcgctg ctagtttcat tttatcttcc
    21841 tactttcttt ttacatatct attttcctgt taccttaaga ttaaatgaga atttaaaaaa
    21901 atgaaactaa cttgctttgt cattatgaat tttttcaaaa aacattctta agaaaaaggt
    21961 ggctatatat agtagttttt ggtcaatacc tttgaataaa aagccatgaa aaacgaatag
    22021 ttagcgctta ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat gaaaacaaag
    22081 catatatttt gctttaaaac caaatgagga atattctaga tgtagaacag gttagaaaaa
    22141 gattagaaaa aggagtgatt ttgatggaga agagatacgg cgtagttcct gtggtaactg
    22201 cttcagacga aaattatgct ccttacttga gcgtgatgat cgcaactgca ttagaaaatt
    22261 gcaataaaac aagacgcata aaattttatg tgattgacga tggtctatca gaatatagta
    22321 aagaaggatt ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc caattcctga
    22381 cagtggaaaa agatatctac gaagacttcc tagtcagtga ccatatcaca actaccgctt
    22441 acttaagaat ttctttacca aacttattag ctaaagagga ttataagaaa gtcctttact
    22501 tagattcaga tgtgttagta ctagacgata tcgtaaaact atatgatgag ccgttaaatg
    22561 gaaaaacaat cggcgctatt atcgatccag gccaagtgaa agctttggaa agattaggaa
    22621 tcgattccga tgacttgtac ttcaattcag gagtgatggt gattgacatt gatcaatgga
    22681 ataaaaaaga aattacagaa aaaactatcc attacctcag tgaaaatggc gatcgaatca
    22741 tctatcacga ccaagatgct ttgaatgctg tactttatga agattgggaa caacttcatc
    22801 caaaatggaa catgcagact tcgctgattt ttgaacgtca tccagcacca aatgaaaaat
    22861 atgaacgtca gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt acaggacatg
    22921 ataaaccttg gaatacgttg aaagatcatc cgtataccaa tttatactta aaaaaattag
    22981 cacacagtac tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat agcagtagtc
    23041 gcaagttgta acacaaaatt cgtaccacat ttagctgcat tatttgtctc agtattagat
    23101 aattgcaacc cgtctaaatt tgttcggttc tatgtgattg acgatgatat cgattttgaa
    23161 agtaaacagc ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga tgtggaattc
    23221 ttgaaaatca ataaagaatt ctttacaaat gtggtaatca gcgatcgtat tccagaaaca
    23281 gcttattatc gtattgcgat tccggaattg ttccgcggca cagaagttga acgtatctta
    23341 tatatggatt gcgacatgat cgctttacaa gatatctcta aattatggcg tcttgacttc
    23401 ggtgattcaa tcgtagcagc tgttgaagac gctgggttcc atcaacgact ggaaaaaatg
    23461 aaaatttcag ctaaatcaat gcgttatttc aactcaggtt tgatgctgat caatgtgaaa
    23521 aaatggttag atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca taatccagaa
    23581 aaattacgat tccatgacca agatgcatta aatgctatct tacatgaccg ttggttacca
    23641 cttcatccgc gttggaatgc acaaggctat atcatggcaa aagcaaaaaa acatccaaca
    23701 gcagcaggtg aacgtgaata cgaagaaact cgcaacaatc cttacattat ccatttttca
    23761 ggacatgtga aaccgtggag caaggatttt gaagggccaa cgaagaaata ttacgaaaaa
    23821 tatgcaggga tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc aaaatatgca
    23881 aaagttcaac caaaaacaga tgtagttgtt aaataataga agacaacaac tgaagagggg
    23941 gagtaggaat ggaaattaca atgtctaaaa atgcagtaga gacgttaatt gaaaaggttg
    24001 gggaaaatac aaaaatcgca ctcgcgctta tcaatgattc cgatccattt ctaagggaca
    24061 aaggtgcttt tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg gaatttggag
    24121 aatatgcgac taaaatcgag catccattat tggacatcta tacatctaaa ttagagcaaa
    24181 attatttcgg taaacgattg aacatggact tcaacaaaca gcttgacagc ttttcattag
    24241 aaaatgaaat cgcagtactc gattataata tcaaattaaa gaactgcttc ttcagctaat
    24301 agtttcgaga aagtccaaaa aggaggtata cattgagata ctactttgtg gatgaagaaa
    24361 agaatgtaat agaaacagat gaaaaaaaat ataattggct cgtacttgat agtgcaaagc
    24421 ccgaagagat tgagcaagca atcgaagaat tcgagcttcc tgatgatatt ttcgtaggaa
    24481 ccgaatatcc ggaagaagtt tctcggatgg agcgccttta tgagacgaaa ctagagcatc
    24541 cattttcttt ggtagtgatt aatctagatc agtcagataa tcgtatcgaa aaaaaattaa
    24601 cgccaatatc ttttgtactt tccgataatc tcttgattac ttgtatcgat agaaaaaccg
    24661 aatttgtgga tcggctgctg acaaagcatg gaaatcattt aaactcgttt gaaaagatca
    24721 tcacgtatac attattagat atttacaccc attacgtgaa agaactaaga gagatgaaaa
    24781 agcgaattga cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa gaactgtata
    24841 aacaggcaga tcttgaaaga gacatcgtat atatcgatca taccttaaga gatcaacggg
    24901 aaacaatgga tcaattatgg aattctgaaa ctttcatcaa acgtttagac gatgaacagc
    24961 tactctatga tgtccatttg agacagcgac aaacagaaaa aatgatcgag atctatcgag
    25021 atttattaga gagtattggt gatctattca acgggatgat ggacaataat ctaaatcata
    25081 tcatgaaata tctagattca gctgcattaa tcatttccgt tccggcgttg ttcgcaggga
    25141 tttggggaat gaatacagga ggattaccgg gtaaaagttc tgctctaggc tttatactcg
    25201 tagtctttgg ctctttgata gcagcagtag ttttaggtta ccatttatac cggaaagatt
    25261 atacaaatta aagaatctat ttaaaacagg tatcaaacaa agaaatttgt tttggcaata
    25321 caatttaaaa actaggagga atacagcaat gacagaacca aaattaatcg acccgcgaaa
    25381 cttgtaccat acggagaaat ttcctaaaca agatcaggat acaccagctt tacagcagaa
    25441 tatgattcct gttcctgatt gtggagaaga atcctatgaa ggcaataatc aactagaaaa
    25501 ccgccgtgta ctaatcactg gtggagattc agggatcggt cgtgcagcag cgattgcatt
    25561 tgctagagaa ggggcagata tcgctcttca attcttccca ggagaagaaa aagatgcaga
    25621 ggaagtagca aaatacgtga aagttgcagg acgtaagatt gtcttactac cttttgatct
    25681 tcgtgacgaa caagcaccta aagaaatcat agataaagcc gtgagtgaac ttggcgggct
    25741 agatacgatg gtactaaacg caggacagca aatttcttgt cagtctattg ctgagttacc
    25801 acttgagcaa gtcaaagaca catttatgat aaacatcatc gctatgtttg ctttagtgaa
    25861 agaagctgtt ccgcatatcc cagcaggagg agcaatcgta acaacaactt ctgttcaagc
    25921 attcaatcca agcgaacatt tattagatta cgcagcaaca aaagcttcaa tcgctaactt
    25981 tacgatcggt ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg gtgtagcacc
    26041 tggtccaatc tggacaccat tacaactaga cgaaggacaa ccttctgatt cgatccctga
    26101 atttggtcag caaagtttac tagaacgtgc aggtcagcca ggagaattag caccagttta
    26161 tgttttctta gcatcgaata aagcaagtta tgttacagcc caaatttacg gagtaactgg
    26221 tggagaagcg atcaacttat aagaagttga tcgtttcacc cctatgagga ggtgcgaaaa
    26281 tgaataaagg tgtaaaaacg atcggagtga tcggaacatt acttttgcta tctgtgcttt
    26341 tgtttgcgat actattaagt gcaacctatc cattgccttt tcagctagag aagtttcgat
    26401 tcttttccat cactaattca tacgtgcaac aatatgtatt ttggcttgcc gttgcatttg
    26461 ctattttgac aattatcacg ttacttgttt tcctgtttta tccaaaaaca atcggaaact
    26521 ttctactgaa acatggtgat ggggagttga cgttggataa aaaagcaatt gaaggaatgg
    26581 ttcgttctca cttgcatgaa gatgaattca tccattcacc aaaagtaaat gtcaaagcaa
    26641 cgaaaaacaa gatcgccgtc aatgtaaaag gggagttgaa acgaacttca gcactcattg
    26701 gtaaaacagg aacattgatg aaagaaatcg aaaatgaagt acaacacata ttgggaacaa
    26761 aagagccagt gaaagtatcg gtgaaataca caggctttga agaaccagct aaacgaagca
    26821 atgaccgcaa acgagtagaa taggggggga ccaaaaatga aagaactatt ttcgacatac
    26881 aagctcccta tcatctttgg ggtacttgga ttgatattag ccgttttatg gatcaccgtg
    26941 ggctttttca agaccttatt gatcttgatc atggcagcga tcggcgtcgc tatcggtttt
    27001 tatctgaaac aaacgaaaat cttagatcac tattttaaat cttaggagga aacatccatg
    27061 aataacaata acaaaaataa taacaataaa atgaacaacc aaaaaccagt gtctgcacat
    27121 ccagacccaa cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa caaaacagca
    27181 ccagtatctg cacatccaga tccagtagtc cctaacgttc aacaaggagt agaagagaag
    27241 aaacatgatg cagaaaagca tgcacctcat catccaggaa ctccagaatc taaagttcca
    27301 gaacaacatg atgcaatcga tccttctaaa cctgtggaag agcaaaaaca tccttctgac
    27361 catgcgaaag cacaagaaaa acttagttct gaccacacaa acggacaagg aaaacttggt
    27421 tctgatcata caaataacca aggaaagcat gattctgaac aggaagattc aatgcgcggt
    27481 gagctgaatt atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat tgaacaagta
    27541 gacggtttat tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa acttgtgaat
    27601 acagataatg taacggctgg gatcgaaaca gaagtcggta aaaaacaagt agcagttgat
    27661 atggatgtca ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga aatgcaagaa
    27721 gtaatcggca aagaagtaaa aaatatgaca catttagaag tgatcgaagt caacgctaac
    27781 gtagtggaca tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt gcaagacaaa
    27841 gtaactgacg cagcgaaaaa aaccggtgaa tttgcttcga atcaaacaga taaagtaaaa
    27901 tcagcagctg gaagcggcgc acaaaaagta aaagaaaata cagaaccacg ggttcagtaa
    27961 taaggaagta aaacattagg aggaacgaaa catgggattt atttggtcac ttatcgtcgg
    28021 aggaattatc ggagcaattg caggagcaat cacgaataaa ggttcttcga tggggattat
    28081 tgcaaatgtc gtagcaggtt tagttggatc agcacttggt caagcattat taggttcttg
    28141 gggaccaagt attgcaggaa tggcaatcgt gccatcattg attggtgcca tcattttagt
    28201 actaatcgta tcattctttg ctcgaaaact gtaaaaagac ttttaaaaag aggtcgtgaa
    28261 aaagctgttc ttcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc
    28321 atttggttgg gcatattatc gaagatgcaa gcttttgaac actgtttata cggaataaga
    28381 ggctgtgaca agacttttgt cacagcctct tatttatgcg ggcaaattcc caattagatt
    28441 tttaaaagtt tttccttaaa tgtgtcttta ttgatacaga gaagcttctt taataaaaca
    28501 atgcctccct aaattttctt gagataggaa aggtgaacat acaaaataat acgaccactt
    28561 tgaaaaccca gatgatccac aaatggatcg tatgatactg atgaagtaaa aagcaatgga
    28621 tcagaaactc aaagagattg aaaaagacaa ggaagaaata atatctcttt tttactcttg
    28681 tctgttttag ccaataatag ctcgtaaagt aatctagaca aacaaataga agtaaacaaa
    28741 gtttcgtaag agcagcaatc gtatcatgtt gctctgctag acgattgacg agcaggaaaa
    28801 atgttggtaa tggaatatag atcacgagta atgaaagaaa aaagtaaaca ataaatggca
    28861 tactattcct cctaattgtg tgagttcgcc ctcattttaa aataacgata tttgtttgtg
    28921 aagctttttc gtgtcgtaac aaatgttatc taatgtattt ttaaataaat aatcattatt
    28981 atcaaaataa t
//
LOCUS       DAPBAB010000030        28033 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPBAB010000030 DAPBAB010000000
VERSION     DAPBAB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.030"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            185..1183
                     /locus_tag="RD444_001624"
     CDS             185..1183
                     /locus_tag="RD444_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7549000.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="RD444_001625"
     CDS             1295..1804
                     /locus_tag="RD444_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDT7549001.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="RD444_001626"
     CDS             complement(1834..2493)
                     /locus_tag="RD444_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDT7549002.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="RD444_001627"
     CDS             complement(2495..3259)
                     /locus_tag="RD444_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDT7549003.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="RD444_001628"
     CDS             complement(3267..3944)
                     /locus_tag="RD444_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDT7549004.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="RD444_001629"
     CDS             complement(3981..5366)
                     /locus_tag="RD444_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7549005.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="RD444_001630"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RD444_001630"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="RD444_001631"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="RD444_001631"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDT7549006.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="RD444_001632"
     CDS             6305..7162
                     /locus_tag="RD444_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549007.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="RD444_001633"
     CDS             complement(7243..7578)
                     /locus_tag="RD444_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549008.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="RD444_001634"
     CDS             complement(7645..9015)
                     /locus_tag="RD444_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDT7549009.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="RD444_001635"
     CDS             complement(9093..9629)
                     /locus_tag="RD444_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7549010.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="RD444_001636"
     CDS             9938..11419
                     /locus_tag="RD444_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549011.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="RD444_001637"
     CDS             complement(11522..12229)
                     /locus_tag="RD444_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDT7549012.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="RD444_001638"
     CDS             complement(12308..12646)
                     /locus_tag="RD444_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDT7549013.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="RD444_001639"
     CDS             complement(12757..13719)
                     /locus_tag="RD444_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDT7549014.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="RD444_001640"
     CDS             complement(13734..15065)
                     /locus_tag="RD444_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7549015.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="RD444_001641"
     CDS             complement(15073..15753)
                     /locus_tag="RD444_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDT7549016.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="RD444_001642"
     CDS             complement(15972..16451)
                     /locus_tag="RD444_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDT7549017.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="RD444_001643"
     CDS             complement(16578..18257)
                     /locus_tag="RD444_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDT7549018.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="RD444_001644"
     CDS             18805..20307
                     /locus_tag="RD444_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549019.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="RD444_001645"
     CDS             20320..20982
                     /locus_tag="RD444_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549020.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="RD444_001646"
     CDS             complement(21083..21286)
                     /locus_tag="RD444_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDT7549021.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="RD444_001647"
     CDS             complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="RD444_001647"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDT7549022.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="RD444_001648"
     CDS             complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="RD444_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDT7549023.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(25250..25864)
                     /locus_tag="RD444_001649"
     CDS             complement(25250..25864)
                     /locus_tag="RD444_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7549024.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(26470..26847)
                     /locus_tag="RD444_001650"
     CDS             complement(26470..26847)
                     /locus_tag="RD444_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549025.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(27147..28028)
                     /gene="galU"
                     /locus_tag="RD444_001651"
     CDS             complement(27147..28028)
                     /gene="galU"
                     /locus_tag="RD444_001651"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDT7549026.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         8325 a         5875 c         4686 g         9147 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag cgccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc
    21421 ccatactttg acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat
    21481 atcaagcgtt tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc
    21541 ttttttatcc aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc
    21601 caaagggata ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact
    21661 tcctactcct aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc
    21721 atctgaggtc atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc
    21781 tttgctgaag cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt
    21841 gaagtcaatc gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt
    21901 gcgatttgca tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg
    21961 tgtcaatagc tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt
    22021 tccggataac atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact
    22081 tggctgagaa gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat
    22141 tgtcaaaaag tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc
    22201 ttcgtataca taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc
    22261 agtcatatct ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc
    22321 tgtagtcaat tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc
    22381 tccacctaaa atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc
    22441 tgaaagttct tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa
    22501 aatcttccca ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc
    22561 aatttgtcga atgattttca tacatttttc ctctttcttt aagtataact gatgatagga
    22621 tcttaaaaaa taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg
    22681 gttcttggag tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta
    22741 ctgcagacct attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc
    22801 ttgcatcttg atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac
    22861 accaatcaag ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata
    22921 agcaagaact gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg
    22981 catagtcaaa gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa
    23041 gatgtacatc gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc
    23101 atgcaaaatc gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag
    23161 aatccctttg gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg
    23221 gaaagaagaa gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat
    23281 tccagcaact aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt
    23341 ttgagtcacg ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt
    23401 atgcccaagc atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata
    23461 atcatctgat ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat
    23521 catcatataa ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc
    23581 accagacaac acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat
    23641 ccaagaggtc gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga
    23701 gtcatatcct aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc
    23761 tgccatcccg aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa
    23821 acgctccagt cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt
    23881 acgcataata ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc
    23941 agatccatac ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc
    24001 gccaaaatca tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata
    24061 gatattttcg ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt
    24121 ttcctgcgta agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac
    24181 atccagtttt tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata
    24241 cgacttatcc agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact
    24301 tgtttcgcca tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac
    24361 gacaaaagtc agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg
    24421 tgtcgataat ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt
    24481 tatttcttcc tttctttttt cagtaatttt gatgaataaa aaatggataa tcaaatgaat
    24541 tatacagtaa aaatgacatt tccacatgaa taatttatta gaaaatcact tttttctatt
    24601 ttgttttttt tctcctatgt atgattattt ttttctcttt ccatttatat taagtatttt
    24661 atttctctgt ttttccttaa aattcttttt ttatttttag attagagaaa atctcgtcct
    24721 gtttttttca ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca
    24781 cttataaata tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag
    24841 gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat
    24901 gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga
    24961 gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt tttatttttc
    25021 tgacactaca tattcttata actcatttct cataactgtg aaacaactta taaaaataac
    25081 aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa
    25141 aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt
    25201 tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac
    25261 ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg
    25321 ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat
    25381 catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac
    25441 tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat
    25501 gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg
    25561 aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg
    25621 caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa
    25681 gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata
    25741 tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca
    25801 gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac
    25861 tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag
    25921 gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca
    25981 caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca
    26041 gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt
    26101 aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg
    26161 gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt
    26221 tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa
    26281 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
    26341 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
    26401 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
    26461 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
    26521 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
    26581 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
    26641 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
    26701 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
    26761 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
    26821 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
    26881 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
    26941 ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat
    27001 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
    27061 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
    27121 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
    27181 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
    27241 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
    27301 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
    27361 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
    27421 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
    27481 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
    27541 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
    27601 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
    27661 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
    27721 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
    27781 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
    27841 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
    27901 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
    27961 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
    28021 accttcactt gct
//
LOCUS       DAPBAB010000031        26895 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPBAB010000031 DAPBAB010000000
VERSION     DAPBAB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.031"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(40..1146)
                     /locus_tag="RD444_001652"
     CDS             complement(40..1146)
                     /locus_tag="RD444_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDT7549027.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1356..2357
                     /gene="ccpA"
                     /locus_tag="RD444_001653"
     CDS             1356..2357
                     /gene="ccpA"
                     /locus_tag="RD444_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDT7549028.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2694..5039)
                     /locus_tag="RD444_001654"
     CDS             complement(2694..5039)
                     /locus_tag="RD444_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDT7549029.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5318..6220
                     /locus_tag="RD444_001655"
     CDS             5318..6220
                     /locus_tag="RD444_001655"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDT7549030.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            6433..8208
                     /locus_tag="RD444_001656"
     CDS             6433..8208
                     /locus_tag="RD444_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDT7549031.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8274..9860)
                     /locus_tag="RD444_001657"
     CDS             complement(8274..9860)
                     /locus_tag="RD444_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDT7549032.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            10111..10335
                     /locus_tag="RD444_001658"
     CDS             10111..10335
                     /locus_tag="RD444_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDT7549033.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10476..12227
                     /locus_tag="RD444_001659"
     CDS             10476..12227
                     /locus_tag="RD444_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7549034.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12227..14011
                     /locus_tag="RD444_001660"
     CDS             12227..14011
                     /locus_tag="RD444_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549035.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14337..15188
                     /locus_tag="RD444_001661"
     CDS             14337..15188
                     /locus_tag="RD444_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7549036.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15285..17165
                     /locus_tag="RD444_001662"
     CDS             15285..17165
                     /locus_tag="RD444_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7549037.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(17205..18107)
                     /locus_tag="RD444_001663"
     CDS             complement(17205..18107)
                     /locus_tag="RD444_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7549038.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18338..19741
                     /locus_tag="RD444_001664"
     CDS             18338..19741
                     /locus_tag="RD444_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDT7549039.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19741..21174
                     /locus_tag="RD444_001665"
     CDS             19741..21174
                     /locus_tag="RD444_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDT7549040.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21319..22245
                     /gene="trxB"
                     /locus_tag="RD444_001666"
     CDS             21319..22245
                     /gene="trxB"
                     /locus_tag="RD444_001666"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDT7549041.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22459..24186
                     /locus_tag="RD444_001667"
     CDS             22459..24186
                     /locus_tag="RD444_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDT7549042.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24201..24551
                     /locus_tag="RD444_001668"
     CDS             24201..24551
                     /locus_tag="RD444_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDT7549043.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(24643..25503)
                     /locus_tag="RD444_001669"
     CDS             complement(24643..25503)
                     /locus_tag="RD444_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDT7549044.1"
                     /translation="MSSDSTEVVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25595..26023
                     /locus_tag="RD444_001670"
     CDS             25595..26023
                     /locus_tag="RD444_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7549045.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26185..26361
                     /locus_tag="RD444_001671"
     CDS             26185..26361
                     /locus_tag="RD444_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDT7549046.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26388..26834
                     /locus_tag="RD444_001672"
     CDS             26388..26834
                     /locus_tag="RD444_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDT7549047.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8510 a         4797 c         5747 g         7841 t
ORIGIN      
        1 aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa
       61 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
      121 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      181 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      241 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      301 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      361 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      421 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      481 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      541 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      601 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      661 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      721 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      781 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      841 tacattgcca ggctgaccat agcgagtacg aagttctttt ccgatgatct cccaagggtt
      901 ttcgctgtct aaatagccgc ggacatcata cgcccaacta ctgttttgag catcttcaac
      961 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
     1021 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1081 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1141 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1201 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1261 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
     1321 cagaaaacat ataaaatatg aatgggagaa cacaaatgga aaagcaaaca atcactatct
     1381 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1441 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agtcatcgat cgcttggatt
     1501 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggggtaa
     1561 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1621 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
     1681 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
     1741 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1801 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1861 aagatgcagt aaataaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1921 cgttgatcga tcctatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1981 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     2041 gtctatctct agccgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2101 atgaattagc catcggtatt ttggatggtt tattagatcg tggcgtgaaa gtaccggaag
     2161 gattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2221 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     2281 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2341 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgagcctg ccgtttaact
     2401 ctgcgttaca aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat attgtgactc
     2461 gtgattcgga gttggcagga gaacaccgtt tattcgggaa aaggggttat gacaaaattt
     2521 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2581 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     2641 ggactgtgac aatactattg tcacaatctc ttattactgt gtattctatt aaatcaatca
     2701 tctttctcat ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc
     2761 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     2821 ggtccatttt tcgcccatag cgattcaact gtttttccag gtgtttggat ggatttttta
     2881 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     2941 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     3001 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     3061 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     3121 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     3181 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     3241 ctgcgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     3301 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     3361 tcttcataga cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg
     3421 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     3481 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     3541 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     3601 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     3661 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     3721 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     3781 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     3841 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     3901 atgtatccat aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc
     3961 gaatggattg tatatccttt attttggatt gtttgctgtg ccttttgata ataagcgtca
     4021 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     4081 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     4141 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     4201 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     4261 gaaagatctt cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg
     4321 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     4381 attcctcttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     4441 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     4501 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     4561 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     4621 gatactaacg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     4681 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc
     4741 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     4801 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     4861 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     4921 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     4981 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     5041 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     5101 cagacaaggc tgtttttggc aggaatttct tttttaaagg agttaaaacg tgagaaaatg
     5161 atctttttta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     5221 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     5281 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     5341 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     5401 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     5461 gaatattgat gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca
     5521 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     5581 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     5641 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     5701 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     5761 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     5821 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     5881 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     5941 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccgaca
     6001 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     6061 aatgtttgac aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa
     6121 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga
     6181 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     6241 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt
     6301 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt
     6361 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     6421 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     6481 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     6541 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     6601 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     6661 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     6721 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc
     6781 gtgaagaaca agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt
     6841 tttacacatg aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag
     6901 atcattatcg cttgtttact agctatttat taccgcaaat acggttctag cagaaaattg
     6961 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     7021 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     7081 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     7141 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     7201 gtctatcagt ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     7261 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     7321 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     7381 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     7441 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     7501 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     7561 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaaccat caaaaagaaa
     7621 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct accaatcgta
     7681 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     7741 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     7801 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     7861 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     7921 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     7981 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     8041 gtttttcttt tcggtgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt
     8101 ttaagtgtct acactggcgc aggcgtgccg ttaacgcaat tcatcgtagt aacaagcatg
     8161 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     8221 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     8281 atttcttcta ccatttcctg caaacgctgt tcatattttt tgtttccttt atataaatca
     8341 ccaatcgtcc atgccaacag gaggaggaca agaacaatga ttacataagc gatgtgatca
     8401 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     8461 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     8521 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     8581 gggatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     8641 ttcaaagcgg tatgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     8701 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     8761 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     8821 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     8881 atcaagatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     8941 gaaggatctt gaacagaacc ggttttgaag acagcgacac ccataatcaa tagcagcgca
     9001 ttgactagaa aagccatcga caattggata ttagagtccc aagtagaaaa acgtacagct
     9061 tgtgctacat cttcttcgtc ttggtgatcg atcttccgag tttgtgaaac tgctgaatgt
     9121 agatataagt tatgtggcat gactgttgcg ccaataatcc ctaatgctcc agttagcgga
     9181 gtttcgccac caatgacagg tgagtcggag aatgtatggc tgttagggat aaagccagca
     9241 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     9301 acgaaaatca agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     9361 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     9421 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     9481 aattcagtca agatccataa aatgacaccc aatgtcttac ttgtgcgtgc tcggattgct
     9541 tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatactg taaaagcata
     9601 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     9661 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     9721 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc cactgttcca
     9781 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     9841 ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     9901 cacctcaaaa agaaaaaatt aaagaaaaag attcggtatg cctaattttt tttcgtggcg
     9961 tgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
    10021 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
    10081 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
    10141 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
    10201 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
    10261 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
    10321 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
    10381 ttttatttcg taatttgtta gactattcag attattcagc aaaaagacaa gcacagacaa
    10441 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aagttaggct
    10501 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
    10561 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag attgccgata
    10621 aaaatttatc agtaaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
    10681 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcggttgg
    10741 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
    10801 aaaaatatcg gactggggat ttgatggctc atgcaacgaa tgatttgaat gcgatccaga
    10861 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
    10921 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
    10981 ctttgctggc cgtcacttca cgagtgctgg ggtcgaaact ccatgatgct tttcgtgatt
    11041 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
    11101 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
    11161 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
    11221 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
    11281 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
    11341 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
    11401 atcgcgtcaa tgaacttttg catgaaaaaa cgcatatcgt ggaacaactg ggtgcagtgc
    11461 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
    11521 aatcaccagc attagaacaa atcaagtttg caatccaaga aggagaaaca ttaggtattg
    11581 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
    11641 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
    11701 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
    11761 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
    11821 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
    11881 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
    11941 tcaaaaatcc tgaattatta atattagatg atgcactttc tgcagtagat gccaaaacag
    12001 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcatttcag
    12061 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
    12121 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
    12181 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
    12241 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
    12301 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
    12361 tctgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
    12421 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
    12481 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcttcatt
    12541 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
    12601 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
    12661 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
    12721 ttcattcatt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttctt
    12781 gccaatttta ggaatagtca tctggtatta ccagaaattt agttctcgta tttaccggaa
    12841 tatgcgggaa aaattaagtc agctgaatac gaaattgaat gaatcgattt ccggcatgcg
    12901 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
    12961 tgagtattta gacacgcgtt atgcgatgat caaaacaaat tcgattctgt taagtccgat
    13021 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
    13081 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
    13141 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
    13201 tggtagtcgt atcttgaaga ttatggatac agaagaatat gcaccacaac aaaaccctgg
    13261 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
    13321 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
    13381 attagtggga cacaccggaa gtgggaaaag ctcgatcatc aatgtgttga tgcggtttta
    13441 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
    13501 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
    13561 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
    13621 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
    13681 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
    13741 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
    13801 aacagaagga ttgatccaag aaggattggc gaaaatgcga caaggacgga caacgattgc
    13861 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
    13921 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
    13981 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtagc attttgacct
    14041 aagcactaga gtgaaaataa aacatagctt cccatatttt tcatctgtga gagttagtgt
    14101 cgtagttacc agatttaaaa aagacgacgt cagctagtca gaagtgccta atagccccta
    14161 agaaagcgtt gacaaaatcg tttgctttcg ataatataaa gaaaacgata caacttggat
    14221 agtgattgcc tgatgcaagc taaacctaat gagtctccca tggtttcatg gacggctttt
    14281 tgggtttttc tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc
    14341 tgattgagaa agtactgaat aacaacgtcg taataacaag ggatgaaaga gatcgggaaa
    14401 tgattgtgat gggaaaagga ttggcattca aaaagaaggt gggtgacccg attcttgatt
    14461 catgcatcga caaaaaattt cagttgacaa atgacggact ttcacaacag ttccaagaat
    14521 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
    14581 aagaggctct gcaagtccat ttaaatgatt ctgtgtatat ttcattgacg gatcacttgt
    14641 atacagctat cgaacgtcaa aaaatgggga tctttgtgcc aaatgtattg ctgtatgata
    14701 tcaaacaact gtttcctaaa gagtatgcca ttgggaaaaa aacagtgaag aaaatctcgg
    14761 ataaatacca catcaagtta tcagaagacg aagccggatt tatcgcattg catttagtca
    14821 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttaccaca acaatacagg
    14881 atattttaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccact
    14941 tttttcgatt cgtcacacac ctgcgttttt ttctgacacg tgttctagat ggagcgaaat
    15001 cagaagatgg agaagtttct gaggaattgt tccaagttgt tcaaaaaaaa tataaggata
    15061 cgtttggttg cgttgagaaa atagcagact ttttaaccgg ggctttcgac taccagatgt
    15121 ccacggatga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    15181 gtaaataaat aaaaaaggag gacaaattaa caagaacgta tgaaaataag ggtagtgaca
    15241 ttcagttggc taaaccttcc actaattaat tggagggaat agaaatggga aaataccagg
    15301 aattagcaga aaacatcgta aagaatatcg gaggaaaaga aaatatccgt agcttgacac
    15361 actgtgtgac gcgacttcgt ttcaaactaa aggaggaaaa taaagcaaag gatgatgttt
    15421 tgaaaaagat ggaagggata gtcaccatca tgaaaagcgg cgggcaatat caagttgtaa
    15481 tcgggaatca tgtgccagat gtctatgcag aagtactgac agtagccggg atttccgatg
    15541 aagtagaaga agaggtttct tcaagtagta atctgttcaa tcgattaatc gacatattaa
    15601 gtggctgttt ccagcctttt ttaggtgctt tagctgcagc gggaatggtc aaaggattaa
    15661 atgctttgtt cttgtttctc aaattttata ctgaaggatc gggtacgtac atcatgctga
    15721 atgggatagg ggatgcaatt ttttatttca tgccagtgat cttagggtac acagcagcga
    15781 aaaaatttca tctgaatccg atggtggggc tcgtgattgg tgcagcactt tgttatccga
    15841 ctgtacaagg agcagcacta caaagtggtt tccctgatgg cagtcctgcg ccttatagct
    15901 tgctgggtct tccggcttat gaaacatttt tgggtatccc atgggtgggc agcagttata
    15961 ccagtagcgt cgtacctgtt atcttcatta tcgcatttgc cggacaagtc caaaaaattg
    16021 ccaagaaatg gattcctgca gtcgtccaga cctttttggt tcctttttct gtgttattaa
    16081 tcgcacttcc tgctggcttt ttgattatcg gtccgattgt cagtatactg acagatctat
    16141 taagcacggg attccaagca ttgatggact tttctccagc tgtgtatggt gcagttctag
    16201 ggtttctttg gcaagtactg gtgattttcg gacttcattg gagtattatc cctcttgcga
    16261 ttatgcagct agcgcaaaat gggtggagtc agatgttagt aggcgtttat ggcgcgagtt
    16321 ttgcacagac agcagtggta ttggcgatgt atttcaagtt caaggatcaa aaaatgaagg
    16381 cgctttgcat gccagctgtg atttcaggaa tcttcggcgt aacagaaccg gctatttatg
    16441 gaatcacctt accgaagaag aagccattta tttactcgat gattggtggt gccatttcag
    16501 gaatctattt gatggcatct ggaacaatca gctacacaat gggcggatta gggatttttg
    16561 gggtggtaaa ctacatcacg gaaacagacg ccagtggtat gtttcaagcg tttatcggca
    16621 ttgctattgc ttcggtaatc gggttttcgc tgactttttt cttttggaag gatgaagcag
    16681 gtgaaacaga tgctatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag
    16741 gacaaatcgc tccactaaat gcagcgaagg atgcagcttt tgcacaagga acactaggtc
    16801 gaggaatctt gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca
    16861 tgaccctgtt cccgacgaaa catgcaattg gaatggtttc tgaaacaggg ctggaattgc
    16921 tgattcatgt cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc
    16981 agcaaggaga aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg
    17041 aaaaggaagg atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg
    17101 atattttgga aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga
    17161 cctgaacggt taggtgaaga atggaatagg cttaactatt ctttttattc cacttcttta
    17221 ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt
    17281 tgaccgacag aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta
    17341 tataacacgt ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa
    17401 tacagtcgct cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa
    17461 tcaccgatac tggtcaaagc taaaattgga acatgatttt gtttcaatat ttctgcggta
    17521 cgaagaatca ctttattttc acccgtataa gaaatcagga tcccgcacat atcgctcgtg
    17581 caattgtacg cttcatagaa tgattcacca aaagtggtga caattgtgac atccattttg
    17641 atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaag
    17701 atcacgattt tttttgcctg gagcagtaaa tacttggctt tttgcagttg gtcatgatga
    17761 atgagagaaa gtgtatcttg aatcgtcatc tgttctaaag aagccatttt atttgcgatc
    17821 gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct
    17881 tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc
    17941 attttttttg ctacacggat gagtgtagac gggtggacat agcatcgctc agcgatctct
    18001 tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt
    18061 tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta gttaccttct
    18121 tcttattttt ttacgtaaat tataactttt ttgaaaatgt tttgtaaacg gatttatact
    18181 atataactta aaatagactt tttttgacaa gatatttaca aaaatccact agaaaacagt
    18241 aacttttgta aatgttactg gaaagaaacg gatttctatt gtttgttttg tttacaaaaa
    18301 agataatgaa agtgtaaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc
    18361 tacgattggt ggaggcagca gttacacccc tgagttgatg gaaggattta tcaaaagata
    18421 tgaggaatta ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct
    18481 tgagatcgtt agtcagatgt ctcagcgtat gtgggatgct tctccgtatg aagtcaaaat
    18541 ctatgcaact ctggatcggg aagaagcatt gaatgatgcg gattttgtta cgactcagtt
    18601 tcgtgtcggt caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat
    18661 gattgggcaa gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt
    18721 ggtcttggca atcgttgaag acatgaagcg tttatgtcca aatgcttggc tgatcaattt
    18781 cgcgaaccca agtgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt
    18841 gattggatta tgcaatgtgc cagtaatggc gatgatgaca gaaccagcaa tgcttggcaa
    18901 gaaaaaagaa gagctgatct acaagtttgc cggtctaaat catttccatt ggcacaaagt
    18961 agcagataat aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga
    19021 caatggattg ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat
    19081 gaatatgatc ccttgtggct accaccgcta ttactaccgt gaagaagaaa tgctggaaca
    19141 tgctttagaa gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc
    19201 agagctattt gaattataca aagatccaga attagaccat aaacctgaac aattgcaaaa
    19261 acgaggtggc gcttattatt cagacgctgc ctgtgaaacg attgcttcaa tctatggaaa
    19321 taaagaaaca cagatcgtgg tatctacaaa gaatgatgga gctgttcctg acctgccggc
    19381 agattgtgta gtagaagtca ctgcctatgt tggcgcacaa ggagcgagaa atgtagcgtt
    19441 tggttctctt cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct
    19501 gacgatcgaa gcagctgtga ctggtgatta tgggacagct ttacaggcgt ttacgatcaa
    19561 tccgcttata cgatcaggaa agacagcaaa acggatcatg gatgagttgt ttattgcaca
    19621 ccagcagtat ttaccgcaat tcaaggaaac aatcagccga ttaaaagaag aaggaatcga
    19681 agttcaagat gaactggcaa gagaattatc aggtaataca catataatgg aggaagtata
    19741 atgggattca gaaaagattt tttatggggc ggtgctacag cagccaatca atgtgagggc
    19801 ggttatgatg aaggtggacg cggactagca aacgtagatg ttgtgccaat cggaaaagac
    19861 cgttttcctg ttatcaccgg aaagaaaaaa atgtttgaat ttgatgagga tcatttttat
    19921 ccagcaaaaa ttgcaatcga tatgtatcat cgttataaag aagatattgc tttgtttgca
    19981 gaaatggggt tcaagactta tcgaatgtcc atcgcctgga gtcgtatttt tccaatgggg
    20041 gatgaaaaag agccgaatga agctggcttg aaattttatg aggatgtctt taaagaatgc
    20101 caaaagcacg gaatcgagcc acttgtgacg attacgcatt ttgattgtcc aatgcatttg
    20161 atcaaagaat acggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgt
    20221 cgagtcatct tcaatcgata taaaggactt gtcaaatact ggctgacatt caatgagatc
    20281 aatatgatcc ttcatgcacc attcatggga gcaggactgt actttgaaga aggagaaaac
    20341 gaagaacaag tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg
    20401 aagattgctc atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca
    20461 tattatccat acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa
    20521 aattatttct tcattgatgt tcagtcacga ggagaatacc cagcttatgc attgaaagaa
    20581 atggaacgaa atggcattca gatcgaaatg gaaccagggg atgccgcatt gctgaaagaa
    20641 catactgttg attttatctc tttttcttat tattcttccc gagtttcgac taccgacaac
    20701 gaactcttgg aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctgcaa
    20761 gcaagtgaat gggggtggca aattgatcca ttaggcttga gaataacgat gaatgatatt
    20821 tatgatcgtt atcaaaaacc attatttatt gtagaaaacg gcttaggtgc agtcgatacg
    20881 ccagatgaga atggttatgt ggaagatgat taccgaatcg attacctggc agcgcatatc
    20941 caggcgatga aggatgcagt agaactagac ggagtcgatc tattaggcta tacgacttgg
    21001 ggatgtattg atttagtatc agctggtacc ggtgagatga agaaaagata cggattcatt
    21061 tatgtcgatc gtgataacca aggaaatggc acattagccc gttcaaagaa aaaatcattc
    21121 aactggtata aaaaagtaat tgattcaaat ggagaaaatc tttcgaacga ttgatcaatg
    21181 atttataaaa gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa
    21241 acgcaagcag aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa
    21301 gtaaaggaga atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg
    21361 actgctgctt tatatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggtgcc
    21421 cctggcggcc aaatgaacaa tacggcagaa atcgaaaatt atcctggatt cgattcgatc
    21481 atgggccccg atctagctta taagatgtat gaaagtgtct caagatttgg gacagaaaat
    21541 gcttatggaa tcgttcaaga tatcaaggat cacggcagcc ataaagaagt catctgtgaa
    21601 gataaaagtt acgaaggaaa aacagtcatt atcgcaacag gctgtatcca ccgtaaatta
    21661 ggcgtacctg gcgaggaaga atttgctgga cgaggcgttt cttactgtgc agtctgtgat
    21721 ggtgcgtttt tccgtaacaa acgtctagta gtcatcgggg gtggggactc agcagtagaa
    21781 gaagccatct atttgacaca atttgcttct gaagtagtga tcgtccaccg tagagatgaa
    21841 ttacgtgcac aaaagatcat ccaagaccgt gcatttgcaa atgaaaaaat ttcctttatg
    21901 tgggatacag tcgttgacga aatcgttgga aacgaaatgg tagtgacagg tgtgaaagca
    21961 cgtaatgtca aaaccggcaa tgaatttgat ttagcagcag acggagtatt catctatgtt
    22021 ggactggatc cattaactgc accttttaga gaagcaggtt tgacgaacga agctggctgg
    22081 attccaacag acaacgaaat gaaaacgaat atgccaggcg tttttgccat cggagatgtt
    22141 cgtgaaaaag atttgcgaca aatcactaca gctgttggtg acggcggtat tgctggacaa
    22201 caagtgtata aatacttgga atctttagaa gaaacaactg cataagttta aaaagagtag
    22261 aagaacgggt tcaattgttc ttttactctt ttttttcgcg tgaaatttac tgaatctgat
    22321 ttatgcgaac acgtccaaca ccttttgata aaagaatcct ttcttgattt aagcgttttt
    22381 tggtcctcat actttcatat ctaataagtg aatgctatac ttattaagaa ccatatttca
    22441 gaaagaggtg ttattttcat gtcttgggaa caagtgtatg aacaatgggt acatgaagaa
    22501 aatttagaag aaaacttaaa aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt
    22561 gaagatgctt tttatgcacc gttggaattc ggtactgcag gcatgcgggg aatcttagga
    22621 cctgggatca atcgaatgaa catctatacg gtaagacaag caacagaagg actagcacgt
    22681 ttcatggata cacaaggtga agaagcaaaa cgccgaggtg tcgctattgc ttatgactca
    22741 cgccatcagt caccagaatt tgcaatggaa gcagccaaaa cactggcaca tcacaatatc
    22801 ccgtcttatg tattcgaaag tctgcgtcca acaccagagc tttcttttgc agttcgtcat
    22861 ttgaagacgt ttgcgggaat catggtaact gcatctcaca acccagcggc ttacaatgga
    22921 tataaagtct acggagaaga tggcggacaa atgcctcctg cagacgcaga tgcattgacg
    22981 aaatatgtcc gtgaagtcag caatccgcta aaagtcgaag tattatctga cgaagaagct
    23041 aaacatagcg ggttgatcac gatcatcgga gaagaagtgg acgctgctta tctagaagaa
    23101 atcaaagcag tgacgattga tcgtgaattg gtagaaacaa tggggaaaga tttgaaactt
    23161 gtgtacacgc cacttcatgg aacagggaaa atgttgggcg aacgtgcgtt gaaacaagct
    23221 ggattcgaac aattcgtact agttcccgaa caagctgtgg cagatccaga tttcactacg
    23281 gtcaaatcgc caaatccaga agaacattca gcttttgaat atgctatacg acttggagaa
    23341 aaagaaggag ctgacttgct gattgcaaca gatcctgatg cagaccgttt aggcgcagcg
    23401 gttcgtttgc cagatggttc ttatgaagta ttgactggga accaaatcgg ggcattgttt
    23461 gctcaatata ttttggaagc acatcaatca agaggcactt taccagaaaa cgcagcggta
    23521 ttgaaatcaa tcgtttccag cgaattgcct acagcaattg cggaacacta cggtgctacc
    23581 atgttcaatg tgttgacagg gtttaaattc attgctgaaa aaatccaaca attcgaagaa
    23641 gaccattcac atacttttat gtttggattc gaagaaagtt atggttattt agttaaacct
    23701 tttgttcgcg ataaagacgc gatccaagca ttgcttttat tagcagaagt agcagcttac
    23761 tataaaaaac aaggcaaaac gttgtatgac ggcttgcaaa cgatttttga aacctatggc
    23821 tattttgcag aaaaaacgat ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc
    23881 tctgctttga tggaaaaatt ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt
    23941 attcaaacag aagactttaa agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa
    24001 ctaacaacac cgccatccga cgtattgaaa tatacattgg aagaaaatgg ctggattgct
    24061 atccgccctt caggaactga gccgaaaatc aagttttata ttggtgtaaa aggaagctct
    24121 acagaagacg cagccgaaaa agtagcctct tatgaagcga ttatcaaaaa gatgacagaa
    24181 gaataaaaaa cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca
    24241 agttggaggt gaatggattg ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat
    24301 tattcaaaat gttgagtgat cccactcgtc ttaaaattct gctatatctg aaagacgggg
    24361 aacaaaatgt gacagcaatc acccaagcag tggaaatgga gcagtctgct gtatcgcatc
    24421 aactacgttt attaagagaa aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt
    24481 tgtatagtct tgatgattct catgtactgg atattttgaa ccagacactc aaacatgtgg
    24541 aacatcaata acgataggtg caaatagata aacactggag tgtgacaacg aaatttcttc
    24601 gttgtcacac tccagtgttt tttaagaaaa agacaaaaat gattaatcgc ttttttctgt
    24661 tgtttcagtt ccataataac tatgatcttc taggtctaaa gcattcaaaa taagcgaagc
    24721 cgtttcttca atagataaag atgccacatt gatcactaca catcctagtt tttcataaag
    24781 atcattcgca aaatcgagtt cagctctaat tttttcgata tcggaataag cagtatcagg
    24841 gtttaatccg taagagcgca tccgttcttt acggatatta tttaaaatat ctgggttatt
    24901 cgttagtccc acgatttttt taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg
    24961 tgggatcaaa ggtaaattgg caacttttaa atttttgtta gctaaaaaca agctcaaagg
    25021 cgttttggaa gttcgggata cgcctagcaa aacgacatct gcttctaaaa aaccacgagg
    25081 atctttccca tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt
    25141 ctcattcaat aaatggaggg caccaggttg ccgaattggc ttgataccgg ttaaacgttc
    25201 aatttctgat atcggtggcg tcatcacatc aaaactaaat aattgatgtt cttcacaaaa
    25261 agaacgtgtt atctcgatca gttcttgatt gattaatgta tgcaggacaa gtgctttttc
    25321 ttctaatgca tcttgcaatg cctgctttaa tgttttttca tctgtcacga acgttctgcg
    25381 gaataggttg aactcaacgg taggatactg agccatagta gcttgcgcta gttttgtcgc
    25441 agtttctcca gcagagtcgg agatgacaaa aaaagtaacg acttctgtag aatcggaaga
    25501 catggataaa acctcacttg catttttttt atgtttatta taccatactc tagaatgcgt
    25561 acttcgtaat cattttgatt tggaggaata agtcatgaaa aaacagacat tgatcgaaca
    25621 atcattagaa acattgaaaa ataacggttt taaatatact aaaaaaagag aagcattgtt
    25681 aacttattta gtgaagcgta accgctacgt atctgctaaa gaagtttttg actatatgaa
    25741 tcaggaattc aaaggggtaa gttatgatac gatttatcgg aatctccatg attttgcaga
    25801 gatcgattta ctggaagaaa ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg
    25861 tcagggggaa atcggccatc accatcattt tatctgtaca gtgtgcggaa caacgaaaga
    25921 aatccatatg tgtccgatga attatttcga agaacagcta ggtggatgca cgatcgaagg
    25981 gcatagattc gagattttcg gaagatgtga aaattgtcaa taagggtata aataaaataa
    26041 catttgataa ttttaaaaaa aagcaagatt tccttgacgg tcaaaacagc attagatata
    26101 atatttacag gacagtcttg ttttttgaga tatatttcaa taacaattac ggttactttt
    26161 taagctcgga gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt
    26221 gacgatgctc ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt acaagaatct
    26281 cgtaaacgtg aattctacga aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct
    26341 agaaaacgca aaaaattcta gttctgtgat gaattggagt tggtagtgtg tcactactaa
    26401 gtagattaaa cgatgacatg aaagcagcga tgaaagcgaa agataaggaa tctttacaag
    26461 tcatccgaat gatcaagtca tctatacaaa acgagcagat taaagaaggt cacgacttga
    26521 cagaagaaga agaactaacg gtgttgtctc gcgaaatgaa acagcgacgt gattctttgc
    26581 atgagtttga agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa
    26641 tagtcgaaaa atatatgccg gaacaacttt ccgatgaaga aattcgacaa cttgttcaag
    26701 aagcaatcac tcaaactggt gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa
    26761 ttatgcctaa agtcaaagga aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac
    26821 tattacaatc ttaattttat catttaaact gcggacagtg attgtccgca gttttttgtg
    26881 ttcgtaaaaa aagaa
//
LOCUS       DAPBAB010000032        25646 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPBAB010000032 DAPBAB010000000
VERSION     DAPBAB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.032"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            133..3660
                     /locus_tag="RD444_001673"
     CDS             133..3660
                     /locus_tag="RD444_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDT7549048.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="RD444_001674"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="RD444_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDT7549049.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="RD444_001675"
     CDS             7448..7789
                     /locus_tag="RD444_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7549050.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="RD444_001676"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="RD444_001676"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDT7549051.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="RD444_001677"
     CDS             9146..11566
                     /locus_tag="RD444_001677"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDT7549052.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD444_001678"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD444_001678"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDT7549053.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="RD444_001679"
     CDS             13000..13737
                     /locus_tag="RD444_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7549054.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="RD444_001680"
     CDS             13751..14566
                     /locus_tag="RD444_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDT7549055.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="RD444_001681"
     CDS             14579..15226
                     /locus_tag="RD444_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7549056.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="RD444_001682"
     CDS             15242..15898
                     /locus_tag="RD444_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7549057.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="RD444_001683"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="RD444_001683"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDT7549058.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="RD444_001684"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="RD444_001684"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDT7549059.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="RD444_001685"
     CDS             18240..18758
                     /locus_tag="RD444_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7549060.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="RD444_001686"
     CDS             18865..19365
                     /locus_tag="RD444_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7549061.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="RD444_001687"
     CDS             complement(19645..20565)
                     /locus_tag="RD444_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDT7549062.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="RD444_001688"
     CDS             20730..21743
                     /locus_tag="RD444_001688"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDT7549063.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="RD444_001689"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="RD444_001689"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDT7549064.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="RD444_001690"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="RD444_001690"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDT7549065.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="RD444_001691"
     CDS             complement(23383..24144)
                     /locus_tag="RD444_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7549066.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="RD444_001692"
     CDS             24333..25427
                     /locus_tag="RD444_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7549067.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25646)
                     /locus_tag="RD444_001693"
     CDS             complement(25513..>25646)
                     /locus_tag="RD444_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7549068.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8493 a         4442 c         5408 g         7303 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcactt
//
LOCUS       DAPBAB010000033        25303 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPBAB010000033 DAPBAB010000000
VERSION     DAPBAB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.033"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..210)
                     /locus_tag="RD444_001694"
     CDS             complement(<1..210)
                     /locus_tag="RD444_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7549069.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="RD444_001695"
     CDS             complement(309..623)
                     /locus_tag="RD444_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549070.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="RD444_001696"
     CDS             complement(691..1185)
                     /locus_tag="RD444_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549071.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1205..1492)
                     /locus_tag="RD444_001697"
     CDS             complement(1205..1492)
                     /locus_tag="RD444_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549072.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(1708..3099)
                     /locus_tag="RD444_001698"
     CDS             complement(1708..3099)
                     /locus_tag="RD444_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDT7549073.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="RD444_001699"
     CDS             complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="RD444_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDT7549074.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3699..3896)
                     /locus_tag="RD444_001700"
     CDS             complement(3699..3896)
                     /locus_tag="RD444_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549075.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3887..6565)
                     /locus_tag="RD444_001701"
     CDS             complement(3887..6565)
                     /locus_tag="RD444_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549076.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6552..6770)
                     /locus_tag="RD444_001702"
     CDS             complement(6552..6770)
                     /locus_tag="RD444_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549077.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6846..7898)
                     /locus_tag="RD444_001703"
     CDS             complement(6846..7898)
                     /locus_tag="RD444_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDT7549078.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8051..8377)
                     /locus_tag="RD444_001704"
     CDS             complement(8051..8377)
                     /locus_tag="RD444_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549079.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8389..8589)
                     /locus_tag="RD444_001705"
     CDS             complement(8389..8589)
                     /locus_tag="RD444_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549080.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8712..9314
                     /locus_tag="RD444_001706"
     CDS             8712..9314
                     /locus_tag="RD444_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7549081.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9369..10535
                     /locus_tag="RD444_001707"
     CDS             9369..10535
                     /locus_tag="RD444_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7549082.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(10624..10722)
                     /gene="rpsI"
                     /locus_tag="RD444_001708"
                     /pseudo
     CDS             complement(10624..10722)
                     /gene="rpsI"
                     /locus_tag="RD444_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(10992..14069)
                     /locus_tag="RD444_001709"
     CDS             complement(10992..14069)
                     /locus_tag="RD444_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDT7549083.1"
                     /translation="MAEEYRFDENELELVALRWLEEVGYTSVSAKELEDSGMSKRKDY
                     KEVVLIDRLKEALVTINPEVSMAVIDEAIKTITVHKHPDMAVNNQTFHRYITDGIEIP
                     TREGNRNVTKRILLFDFNEPRNNDFLAVNQFIVEEDNQRKRTDIILFVNGLPIVLFEL
                     KNATDEAVGISDAYNQLQTYKQEIPSLLTFNEFMVASDGVQAKVGTLSSNEERFMVWR
                     TIDGKVEHGTASSQLEVLIKGMCNPEVLLDLLHHFILFKTDGDKTIKILAAYHQYHAV
                     NRAVEEAKRAVSTKGDNKIGVVWHTQGSGKSLSMVFYTGKLVQELNNPTVVVLTDRND
                     LDDQLFLTFSQSSDLLRQTPKQANDGDELKELLSVNAGGIVFSTMQKFAPKEGNQMDV
                     LTDRRNVIVMADEAHRSQYGFSADINAKDGSIKYGYAKYLRDALPNASYIGFTGTPIS
                     SADKSTKAVFGDYIDVYDMTQAVEDGATVKIYYESRIIPVSLPDGLDIDDLVEDIVEG
                     QEDDVASKTKAKWSRIEAITGSQPRLEKLAQDFVKHFETRQEASFGKSMIVTMSRRIA
                     IDLYQEIIKLRPEWHSDDDDKGKIKIVMTGSSSDPDYWQPFIGNKTRRDKLAKRMKDD
                     SDELQIVIVRDMWLTGFDVPSMNTMYIDKPMQGHNLMQAIARVNRVFKDKTGGLIVDY
                     IGIAPNLKNALQEYTPNDQKQAGIDTQVAVNLVKEKYDLITNTILDKFDYSDFNSTDY
                     TKKLQVITNAANYILGLDDVEDVKRFKDLVTEITRAYALCVTEEEVQQYNAEIAFFKA
                     VKVYLIKLNFVDGVKSPQQIDEELKQLISKSVVTEDIVDIYDSLGLEKAELSILSDKF
                     LEEIKEMPQKNLALQLLENLLAGKIKSIQKTNVVQAEKYSELLKKSINKYNTRGITSE
                     IAIRELIELAKDMKTMKEEGEELGLTVEEKAFYDVISKNSDEDEQVLKDIAVGIAKTI
                     EENMTIDWTKRSSEKARMRVEIKRFLRKNNFKTDLETIDLVITQAELMAENLI"
     gene            complement(14073..15329)
                     /locus_tag="RD444_001710"
     CDS             complement(14073..15329)
                     /locus_tag="RD444_001710"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDT7549084.1"
                     /translation="MKYEIIKLQDVVDKLGDGLHGTPIYDNNGEYYFINGNNLDDGKI
                     VFKENTKKVSREEYHKYKKDLNDRTILVSINGTLGNVAVYKNEKVMLGKSACYFNVIE
                     NVNKDYIKYVIMSPDFKKYIEQHYTGTTIKNVSLKTMRNYTFKLPSENHQLLVSKILS
                     SLDSKIELNNQIISNLEELASTLFKRWFVDFEFPDENGNPYKSSGGKMVDSELGEIPE
                     GWEITELKSIVDVVDNRGKTPPLEKDKTDFPIIDVKTLSGNGRVVNYNKAQKYVSEET
                     YSSWFRSGHPKINDILISTVGSIGEIKMFLTDKGTIAQNVVGLRAKKHLEYHLYQFLL
                     YNKSNLITYNIGSVQPSIKVTHLLKHKVLLPNKKDLNKFNSVMIGLTDKIINLSKEIN
                     SLTEIRDSLLPKLLSGEIELTEDEGV"
     gene            complement(15319..16842)
                     /locus_tag="RD444_001711"
     CDS             complement(15319..16842)
                     /locus_tag="RD444_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDT7549085.1"
                     /translation="MVSAELKIEDKLWAAADKLRGSMDASEYKNVVLGLIFLKYVSDS
                     FEEKYEELTKDEYADPEDKDEYLADNIFWVPAEARWEKINRDAKTPKIGETIDEAMIA
                     IEKENPSLKNVLPKNYSRPQLDKTRLGETVDLITNIKVGDSENRKTDTLGRTYEYFLG
                     KFASAEGKGGGEFYTPRSVVSLLVEMLEPYKGRIYDPCCGSGGMFIQSEKFVEKHQGK
                     IGDISVYGQEFNPTTWQLCKMNLAIRGIDGNIGTHNADTFQNDLHKGLRADYILANPP
                     FNISDWGQEKLLEDSRWKYGTPPKNNANYAWIQHMVSKLAPEGTAGFVLANGSMSTST
                     KDEFEIRKNLIENDLVECIVALPSQMFYNTQIPACLWFVTKSKAKKNERNHQGEILFI
                     DARNEGFMADRTTKEFSEEDIKKLADAYHAWKGTNDKEYTDVAGFCSSASLEIVKEQD
                     YILTPGRYVGLEDEVEDSEPFEEKMERLTALLSEQFIHSHQLEEDIRKALGGIGYEI"
     gene            complement(16923..17144)
                     /locus_tag="RD444_001712"
     CDS             complement(16923..17144)
                     /locus_tag="RD444_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549086.1"
                     /translation="MNTKRTYVEIEKDFHHLRKKNYYPKQYNEKLQALMDELKENYDF
                     SVYTEENSRAIALHYHMSQKFQTDKPTLF"
     gene            complement(17226..17444)
                     /locus_tag="RD444_001713"
     CDS             complement(17226..17444)
                     /locus_tag="RD444_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549087.1"
                     /translation="MKDTVTIIKSNLSYQQAIFSLLALDSLPNDYVGNQIFRENVQTI
                     VEKTLENDGSELIIIETNSNDIEAIAYF"
     gene            complement(17869..18696)
                     /locus_tag="RD444_001714"
     CDS             complement(17869..18696)
                     /locus_tag="RD444_001714"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549088.1"
                     /translation="MLNQIQRDRILGKMTASQRNVLFEYKLKDRKNYFLNQIYLKDTD
                     WEFIDYKESLHYQDKDVSKKERLFCQCGREIKYQCVLRSNKNGELINLSLHHLEKHTS
                     IPKSIAKEIEQGILELDEVLDDILQKFDKGERFPQWYKNFYRKYQCILQVSEPYRERM
                     RMFIKEDLPISTSDELELREKSNLYLKELEKPIVITDISDKRFLKDIADYINSREKGE
                     FYILHVAEYLSAKNYDGALDFGDISQAVDILTEQFPTILKTLSLPMKEKISDEKIII"
     gene            complement(18689..20101)
                     /locus_tag="RD444_001715"
     CDS             complement(18689..20101)
                     /locus_tag="RD444_001715"
                     /inference="COORDINATES: protein motif:HMM:NF014814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549089.1"
                     /translation="MRMGERIPTRKLGESDLEYYVRRLTYNMIFHRIISYEIRDSEGD
                     IHYESIDFLNTEEALMNLDYKNVYENVFLSINSFRYCSRREENLRAVNAFYCDIDTEK
                     KWCMKSEDFWQQKVIPFLEAKQFPFPSLVVNSGRGLHLYWIITPVEVNHPSVTFLYKS
                     IQTKLSRLFKELKADYLIDCSRHLRIAGTINKKEGKENQHVSIIYVDDEKDYELSVFR
                     DYFQHQFDMDKEKQKKKNNMILIDYPIKTSYRSKRKSQKKDSDTFSGVRKKFSKRSLY
                     TTRIHDLNQLIKQRNYQLTGHRNDFFFIYANLLMELQYSEKSIMDTVESWNATLVNPL
                     SDSEIKRIVKSSFSKNEAYKFTNTYLIQKLNISEKEQNSFKTMINPQIKKKRKNKKER
                     ERYVQKKKIEEKRQKENMRKKEAYTSTKAMNQEKKQKLINRAIELRKKGHTISAIAQQ
                     LHLSESTIKRYIKKGGSACA"
     gene            complement(20963..21199)
                     /locus_tag="RD444_001716"
     CDS             complement(20963..21199)
                     /locus_tag="RD444_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010776246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549090.1"
                     /translation="MDELAKKKFVQHKTKKTYYQSNATIPKIVVNQLANVLYKEFEEL
                     AGMEQEKLLLSEELVMALWNKYMERVNRELLEEV"
     gene            complement(21192..22628)
                     /locus_tag="RD444_001717"
     CDS             complement(21192..22628)
                     /locus_tag="RD444_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549091.1"
                     /translation="MIKTNKWIKLTSVSFLILVVLSACDKKQDIQQSEGSSQSSTLES
                     TEQIKEKESIIKERTATDYDAIYVQNGLVIAQTGDDSSYNWTIYDTELNEKNSILNAD
                     MKRDELAHSLETGYSKKVNGTAVIEQDVHEDEVYSTYLDENAELAKISLGERTIDNDV
                     SINTLTSGKYEIIPYRDWSGVTINYLANGYREKRFSTAHGSMSGDINKNGYFTLLDQM
                     NDKKTVNDTSVQIYDAKLKDSGDNSSGVKNIATKTINTVKLDTATNINDKNRLFIRQY
                     TSGLVDDPAIWTFYDLDSDTSYNYPSNINIIKFTDYGIETPIGDGEFLGDIGEAENYI
                     FEDLNEKVTPDDSTPSSIDFTPRKKGIISSDGTVIKEPFAFEIKKPNINGIAPYKLEK
                     EDKYGLINVNTGEIIKEPFAHTIQQYNDNGLSAMKESEGGKYSIINEKGNVLEESEYI
                     YISPLTNDNLASYREEVNGSLGVLKING"
     gene            complement(22642..22857)
                     /locus_tag="RD444_001718"
     CDS             complement(22642..22857)
                     /locus_tag="RD444_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase, SecE subunit domain
                     protein"
                     /protein_id="HDT7549092.1"
                     /translation="MALKGGKIFQEMIEVPDKQEGGCLTACISWLIGFAIIIGILVLG
                     FNFLMSHPIFLFGFIAVALFIGVKVSK"
     gene            complement(23058..23630)
                     /locus_tag="RD444_001719"
     CDS             complement(23058..23630)
                     /locus_tag="RD444_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7549093.1"
                     /translation="MTKTIGYIRTSKNSQNLGVEVQKEALIRYKIDVFFIEQVSGRKE
                     NRKELDKALRELEKGDTLIIYKIDRLGRSTKQLVNIMSELQERGINLISIKENIDTST
                     PMGKVIFTILSAIAELEADYISERTKEALAILKENGVRLGNQGLDRRTVNKIKKLYTE
                     TDLTQKEIAEKCHVCIKTVYNVKKRYQLTR"
     gene            complement(24043..24258)
                     /locus_tag="RD444_001720"
     CDS             complement(24043..24258)
                     /locus_tag="RD444_001720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549094.1"
                     /translation="MEFKDFPFGRHLKKLWRFPFDFLKNSIKNDRVLKKLCQKLSFCY
                     HLIFGKQPFIKDDVGVTEPKKKGQCRA"
     gene            24366..25301
                     /locus_tag="RD444_001721"
     CDS             24366..25301
                     /locus_tag="RD444_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549095.1"
                     /translation="MEGVNMNTPIVNKRAIDSEELFQIINNAEGIYQSTLEKMLLCNR
                     ISLASRLDTLKRQKWIQQERFHKQFVYSNNFDLKNLKTLDAQANIIQKLHTFNIYVDK
                     ITVFTNSKKQKELFLSTHSSGRTCFKTITRLKQQANQLINQLPYESAESDFFVECVKN
                     ELTKFPILASSMTEESKNSYYTASLDTIEILTVPTIEFLPFLKSKLDDFSYRNIERNT
                     HYIREDILVYIEQIDKLIYFKKNEHREYEFNQIISIMDFYYFLAKYFHSKDSVYFSLS
                     KDEFDNAHTLYRKSEENKQKFNTVQIKKDKQKAQS"
BASE COUNT         7803 a         4695 c         3290 g         9515 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttttattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattc gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ctcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 accatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttctag attgtttatc
     2341 tcttcctaaa gtaattaact cacctctacg ctcatagaaa tttacacctt gtttgtaaag
     2401 aacatagcca agtttgttat ttgtttttga aaattgataa acttcaatgt tatattcttt
     2461 tgccaaatga tccgaaaaat cttcaaaaga attcacttcg catttttcca acgttgcttc
     2521 gatcgcattt tgcacaaact cttgataatt aatttttcca tatttcatct tagaaggatc
     2581 gtttgaataa agattggttc tcttttctaa gttttcatag tactcattat tcactgattc
     2641 aagaccatgt tcattgagga cttgatcgtt aatttttcgc attagttcta aatctttagg
     2701 gtcaatacgc aaccttttac atgtttccat gttaatatta ttaatgacaa gatgactatg
     2761 aaaatgatct ttatcattgt gacttgcaac aatgatttgc gaatttggaa aagctttctc
     2821 tgcaaattcg acagctattt cgtgcatttt atcaatactt aactctttag aagtactctt
     2881 atcaaacgaa tgaataacat gaaacgcttg tgctatatct ttagatttat tcatcttggt
     2941 acgtactaaa ttcatttctt gcagtgcttc ctcactatca tcactacaat ttattcctga
     3001 gactgcaaca catttttctt tttttgaagg attagctgca tattgtaata gcgaggataa
     3061 atttttcttg cgtcctttcg atgttatagt agctcccaaa gttgtttcac ctcatctttt
     3121 aaacttgcaa taatctgaat tgagtgaatt aaattccgat caactttttg ggaatactct
     3181 tctttcgtac ccggtctagt ttgcttttct atccaatatt cgaaatattc tttttctact
     3241 tctgataatt taaaaaaaga gggggcactc cgattggact ccaaaaaaat gtcatctgga
     3301 taactaatca tcaacttttt tgctatgcta ttcgcaagtt catctttaaa aatgttgtaa
     3361 cggctagcat caaaactaaa atgtaggctc ttttcttttt tatcttcaga ttcttctcta
     3421 aaatatttag caatgatatt taatgccttt accgcttgat tcaaattgtt accaatttga
     3481 tttaactgtt tggttaaaac taccctatca tcttcagcta acaacggcgg ttcatatttg
     3541 agtcccttta accgctcacg gcaaacgtct gctctagaaa ttcccctttg ttttgcaatc
     3601 aatgtcagtt caagataagt ctttttatcg acacgaaaat ttaaggcctc actatgtttg
     3661 cgattttttt cgtacatttt ttgtttatca tccattgatt atcccctcac atttttttct
     3721 agagagcaac atttgaaatt gctctctatt tcatttttca tctttaattc ttcttgaatc
     3781 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgcgttctt tactgattca
     3841 cataattcgt caagttctaa gaaatcatgc gtactaatct ttagaattaa ttgcatgaca
     3901 atccccccct tttatttctt aataaattat cgaaaagtgg attaggatca ttaagtgtat
     3961 ctctgaataa ctcattgtct tcataagtga atcttcgtct tgattttgat cccttatcat
     4021 tttcacggat ttcactatca ataatcactg tttcatgatt tgaatcaaat tttttcgctg
     4081 actttctgtc ttcaacgata ttgatttttt gtgttttttc tttcatttct ccactatctt
     4141 tttgaatgtt ctcccgaaca tctactgttt tgaatccttg actacttgta gaactttgta
     4201 cattttctcg gacatctact gtttttggtt gacttgcact gctaccttgc acattctctc
     4261 ggacatccac tgatttaaac ccttgactac ttgtagaatt ttgtacattt tctcgaacat
     4321 ctactgtttt tggttgactt gtactgctac tttgcagatt ttctcgaaca tcaattgtct
     4381 tagtaccttg agcatttgaa gaactttgta aattctctct tacatcaaca ttccgtccaa
     4441 catgaccatt cagtgaacct tgaatatttt ctcggacatc tactgatcgg ccaccatgac
     4501 tacttgtaga attttgtgga ttttctttga ctaatttttc taatcctgta tttgaatttt
     4561 tagcttcttg aattctttct ttggtcaacg cttcttttcc gtcaatatta gcgcctgtcc
     4621 gcccttctac acgtttcctt aacattattt cctcgcgatt cattgcttca ggcgtgtatt
     4681 tcgcttcatg acgcttctga ttaattaatt cttcagcaga cttagcttga ccgtgcatag
     4741 catttttacg ttcttcaata tcttgttgga ctttttgctg aattgcgctt gcaggattat
     4801 tattcgattc agcattattt ttcattgctt cacgcatacg ttcagaacta gacataattc
     4861 tatcggcatt ttcaacttta gcaccatctt ttctatctga aagacgtgtt ggcacggaat
     4921 cagcatattt tccagtttca tcttctttat ttttagctaa cataggtttg ctatttttat
     4981 taatagcgga cactgtacca tcatctaaac cttctttaaa cttattcttg gtatcattag
     5041 ctatatcacg catactctta aatggttttg ttgctctttc actagccatt gtagctccat
     5101 ctgaagctaa accggataat ccacgttcat gggcatatcc caaagctctt ccagttttgc
     5161 cagctttttt tgatagagtt tcgccgaaat tcttttctgg ttttcgatct tctccttctc
     5221 cgcttccttt agcacgatta aatccatttg atccttttct aaacagcatt gcgcccattc
     5281 caaaagcaga agcaagttgc ccatagcctt ctttgagtcc aatatcaaca ccaaagtatc
     5341 tcatgactgt tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt
     5401 ttataaaaat atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa
     5461 acattgataa cccaaatcct tgaaacccaa cagttaagta acattgtaga atatctgata
     5521 gaacaacttt tgaacgctgt cctgtctcaa tatctgttgc aaaaacaaga acacctaaca
     5581 cacgtttaaa tgcaagttct agtattgctg taatgatcac aaacgcgctg aacacatagg
     5641 cgattgctaa cgctgtcaac ccaattgaaa tcccccatag atttgcttgg tatcggtaat
     5701 agcctgattt caagctatca gaaaatgttg ataagaagtt gtcagaaaac tttgtagcaa
     5761 caaattcatt attagaattt tcaactaatt cataacgaag attgtccgca attggatttt
     5821 ctttttccaa tttatcaatc actttatccg ttaacacttg tgtgagatcc gtctgttgaa
     5881 aattttttcg tgttaaatta tttcgatcct ccgttttatc aatgcttgag tactgatctg
     5941 ttttattaat atatgctaaa tctgttactc cttgttttat caatgaccaa gcgactccgt
     6001 catctgaatt tgtaatcgta gtagagtcct tataaaatgt tttagcaaat tgaataccac
     6061 tagaaatcat ggttggcatt aaaagtatta atgctataga catcactacg ttcattccaa
     6121 ccgattttaa atccactgtt ccttttccag taatcatttt gtaaccaaca aacatcaatg
     6181 aaaggatcat taatccaatg acaattgtat ttacggctga ttgataaaca ttatttagcc
     6241 ctgtactttc aatgaaattg aacaaatcca gcacatctgg aatcatattt tgaatcgaat
     6301 ctgtcacagt aaacatgcct tttaccacag cccatccgag ccaaatcccg aactgactaa
     6361 ggaaatctcc attgtacttt tttaggtaag gtctataaag agaattattc aaaaactctg
     6421 tttgactttt tgtgtcatca aaccaattga aaatactcgc atgagcaata ttacttgtaa
     6481 aaataatcat agctatcaga acaagaaaat taacaaccaa ccaatttatt tttttattgg
     6541 caatcttttt ttcaattatt ttcatcgtta cctcccttag cccattttct gaggctctca
     6601 gagagcttct gagccatttt ttgattaatt atgacatgag ttacacaatt atcattttca
     6661 gctcttaaat cgagaactat agtgttgaag caaatagtct cgattaattc gagactatct
     6721 gtattttgat ctagacacca aacttgatct attaaacgat ttttaatcaa aataacccct
     6781 cctatattca aagttctcaa atgaagtgga gatacccctc catttcattt gactcaattc
     6841 tattcttatt gcttatctat tccttcgctg atttgcaatt caattagttc taacgcttta
     6901 ttttttagag aagcaaagat ataatcgtct ttttgtttga tacgatgatc tgtttttgct
     6961 cttcgtatca agtcacgtaa agtcattgat agctgttctc taacatgatc tacaaaatac
     7021 ttgttattac aaatttcata gataattttc agttgaattt tttgttcttt ttcagcagat
     7081 ctaatcgcat atttgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta
     7141 gccagtttta agcaattcac aatactttct ccccatattt gtttaagaag attttcttca
     7201 ataaatgttt cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaga
     7261 agatcatcat tatgtgattc tttaatatct ttactatctt tataagactt atataattct
     7321 agttcatttt ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg
     7381 catagttcaa attttgaatt atcaacagaa tattgatttg ttccatagtt caaattttga
     7441 actatcaagt ttttttgtct attttcttga tgggttatct tattaatttt ataaacatca
     7501 tctttgcaca gaacaggctt taacaaataa taaaaatttg ggaacatttg cattttactt
     7561 ttattgaagc cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct
     7621 gataagattg tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc
     7681 aaaaaaattt tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct
     7741 tttagtaaca tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg
     7801 tttaaaaaaa taggatattg atagtataat tctgcatata tatcagaatt agaatagcct
     7861 tgcttttcaa ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt
     7921 atttgctata atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg
     7981 atctgcaaac tgacgggaac actcttttta attttttcta cttcatcaaa tttaaaaaac
     8041 gaggtgtttt ttaaatttga tgactcaata tccatttttt gcattcttct tttgaaaaca
     8101 actccatttt ttctaataaa ataactggca atcccattct tttaaatttc aaaaaagttg
     8161 tatatgatac attcaaatat ttagccatct cttttccttt tagaaatggt ctttctaatc
     8221 cggtatcaat tcttgcttga tcaatagcct cttttgtcat ttcaaaaaca taatttttca
     8281 attgattcgt ttgttgttta tctaacatta ctgtaattcc tgcatccatt atttttctcc
     8341 tctcattttc tatttcttca atcagccaac tcgccaagtt gtatttattt agattttcaa
     8401 atcattaagt aaccaattta ccaacttttg ataagtagat gctctaatac tttctttgct
     8461 cccattttcg attttgacat atgttttccg atttatacct atcaattttg ctgcatcgtt
     8521 tttagtaagt ataagatcgg ctctatgtct tcttaagctt ctaatgaatt gatcattaag
     8581 agttatcatt cagcgcctcc tttcaaatta ttcactttgt ggtttttatc ttaataagca
     8641 cattttaaac cacaaaacga gtaatatcaa ttaaaatatt actcaatttg tggttttcat
     8701 gctataatag tttggaattt actatagggg gcgttacttt gaatagaata aaattttata
     8761 ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt aactatacaa
     8821 ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca tggaatatcc
     8881 ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa acaaattcca
     8941 tagaacaacc taatcttgat agaataaaaa ctgctcgttt agaaaaaaag gacaaaatag
     9001 tcaagaatat tgccagtctt gactcagatt attttactga attatctaat ctatactttt
     9061 ccatgacttc tttacttgaa gtcaaacttc aagaagatga ctccgacttt ctttttttaa
     9121 aaagtattct agaggactcc tttgaataca cgattctatt tgccaaacaa aagaagttta
     9181 ttaataaagc tttaaactta aatatcgatg aaagaaaaga gttagaatct gagttatttg
     9241 gcaacttaac ccaagtacag gttaacaatc taaaaagaga gataacagct cgattaatat
     9301 caaaacttca ataaaactaa ttattcaatc agccaactcg ccaagttatc agattggaga
     9361 gattagttat gtcaatacaa aagtacacaa caaaaagcgg tgcacgatat agtgtccgct
     9421 tgtatgttcg taaagatgaa aatggaaagc aagaatacta tgtaaaaaaa gggtttaaaa
     9481 ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa gataccggct
     9541 atcataagcc ttctaatgac ttgtttataa cagtgtatga agaatggttg ccttcttata
     9601 ggaatcgtgt acaagaaacg acatatcaac gtacaaaaga ccttttccgc ttgcatatac
     9661 taccaaaatt cgggaataag tccatttcta agattactcc tgttcactgt cagaatgcag
     9721 tcaatgaatg ggctttaaaa ttcaagaata tcaaacagct taagtcttac agctctcaaa
     9781 tatttgagta tgccattttt gcagagctat tacaccgaaa tcctatggca aatacaaatc
     9841 taccaaagcg ggaaaagaaa gaaaatgaaa attacttttc attagaagaa ctaaaagctt
     9901 tttgggatat tctcaataaa gaagagcctt taaaacatat tttaatcttc cagcttttga
     9961 ttactactgg tatacgtaaa ggagagctag ctgctcttca ttggtctgac atttattttg
    10021 aagaacagct aatgtatgtt agaaagagct atgcaactat tcgaaatgaa gagaagaatg
    10081 caagaagaaa aactgtcagg attcaaaaaa atacaaaaaa tatatcttcc gaacgaatat
    10141 taccaattga tgcacaaact attgaaatgt taaaaatctg gaaaaaagaa caagctcgag
    10201 agcttttgca gtttggcata aatacaaata caaaaaatca acttatcttt acctatgtaa
    10261 atgcagacgg tgacattaat caacctttgc atgcggacta ttcaaataat atattgaaac
    10321 gtttagagaa gaaatataaa tttaagcacg ttactattca tggattccgg cacactcacg
    10381 ctactcttct actagaaggt ggagcaagta tcaaagaaac acaagatcga ttgggtcata
    10441 aaaatgctga aactaccctt aacacttatt ctcatgttac tgaaaaagct caacgaaatg
    10501 cgttagaaaa attcattgct tacactggtt tataatattg tcattacctt tctcattacc
    10561 agaacgaaaa aaagacacct tctcgcaatg agaaagtgtc tgaaatcgtt ggtataacaa
    10621 cgattaacgt tttgaaaact gtgaagcttt acgtgctttc ttaagacctg gttttttacg
    10681 ttcaaccata cgtgcgtcac gagtaagcaa gccagcacgt ttaaagcaga acggaaatct
    10741 ttatatatca acgtttacaa tggtttttct caccatcttt tatactttag aatacagtct
    10801 taaattcttc aatttacctc aaattttcaa aatccagacc caaaaataaa atagatttta
    10861 gccacatgta tcaagggatt agataacatt tcaaaaataa aatagaccca atttattaaa
    10921 atatgaaaaa aatttctatg ttcgtgggta aaataaaaaa gcctaaaatt aaggcttttt
    10981 tatttttacc tctaaatcaa gttttctgcc attaactctg cttgtgtaat cactaaatca
    11041 atggtttcta aatccgtctt aaaattattt tttctcaaaa aacgtttaat ttcaactctc
    11101 attcgggctt tctcagaact acgttttgtc caatcaattg tcatattttc ttcaatagtt
    11161 ttagcgatac ctactgcaat atcttttaga acttgctcgt cttcatcaga atttttagag
    11221 ataacatcat aaaaagcttt ttcctcaaca gtcaagccta attcttcgcc ttcttctttc
    11281 attgtcttca tgtcttttgc caattcaatt aattcacgaa tggcaatttc agaagtaatc
    11341 cctctagtat tgtatttatt tatggatttt ttcaatagtt cagaatattt ttctgcttgt
    11401 acaacattcg ttttttgaat agactttatt tttcctgcta ataaattttc aagtagttgt
    11461 agagcaagat tcttttgtgg catttcttta atttcttcta aaaacttgtc agacaaaata
    11521 gacaattcag ctttttctaa tcctagagaa tcgtaaatat ccacaatatc ttccgttaca
    11581 acggacttag aaattaattg cttcaattct tcatcgattt gttgaggaga ttttactcca
    11641 tctacaaagt ttaatttaat taaatatact tttacagctt taaaaaaggc aatttcagcg
    11701 ttgtattgtt ggacttcttc ctctgtcaca cacaaagcat acgctcgagt gatttccgtt
    11761 actaaatctt taaatctctt aacatcttcg acatcatcta atcctaaaat atagtttgca
    11821 gcatttgtaa taacttgtaa tttttttgta taatctgttg aattgaaatc agaataatcg
    11881 aatttatcta aaatcgtatt ggtaatcaaa tcatattttt ctttgactaa attcacagcg
    11941 acttgggtat caatacctgc ttgtttttgg tcatttggtg tatattcttg tagagcattt
    12001 ttcaaattcg gtgcaatacc aatgtaatcg acaatcaatc cacctgtttt gtctttaaat
    12061 acacgattta cacgagcgat tgcttgcatt aaattatgcc cttgcattgg tttatcaata
    12121 tacatggtat tcatgctagg aacatcaaaa cctgtcagcc acatatctcg aacaataaca
    12181 atttgaagtt catcagaatc atctttcatt cgcttcgcta atttatcacg tcttgtctta
    12241 ttaccaataa aaggttgcca ataatctggg tcactacttg aacctgtcat tacaatctta
    12301 attttccctt tatcatcatc atcagaatgc cattcaggac gaagtttaat tatttcttgg
    12361 tataaatcaa tcgcaattct tctagacatt gtaacaatca ttgatttacc gaaagaagcc
    12421 tcttgtcttg tttcaaagtg tttcacaaaa tcttgagcca atttttctaa tcgaggttgt
    12481 gaacctgtga tagcttctat ccgactccat tttgctttcg tttttgaagc gacatcatct
    12541 tcttgacctt ccacaatatc ttctactaaa tcgtctatat ctaatccatc tggaagtgac
    12601 acagggataa ttcggctttc ataatagatt tttactgtag ccccatcttc taccgcttga
    12661 gtcatatcgt atacatctat ataatcccca aacacagctt ttgtagactt gtctgcacta
    12721 gaaataggtg ttcctgtaaa accaatgtag gaagcgttag gtaaggcatc tcgtaagtat
    12781 ttagcatatc catatttgat tgagccatct tttgcattaa tatccgcact aaaaccatat
    12841 tgagaacggt gagcttcatc tgccattaca ataacgtttc gtctatcagt caatacatcc
    12901 atctggttac cttctttagg tgcaaatttc tgcatggtac taaacacaat gccccctgca
    12961 ttgacagaca ataactcttt taactcatcc ccatcattgg cttgttttgg tgtttgccgt
    13021 aataaatcac ttgattgact aaacgttaaa aacaattggt catctaaatc atttctatca
    13081 gttaaaacta caacagtcgg attgtttaat tcttgcacca atttcccagt ataaaaaacc
    13141 attgataggc ttttacctga cccttgggtg tgccaaacaa ccccaatttt attatctcct
    13201 ttagtgctaa ccgcacgttt tgcttcttct actgcacgat tcactgcatg atattgatga
    13261 taagctgcta aaatcttaat cgttttatcc ccatcagttt taaataagat aaaatgatgc
    13321 agtaaatcaa gtaacacttc tggattacac atccctttga ttagcacttc taattgacta
    13381 ctagctgttc catgttccac ttttccatca atcgttcgcc aaaccataaa acgttcttca
    13441 ttacttgaca aagtccctac ttttgcttgc acaccatcac tggcaaccat gaattcatta
    13501 aacgtaagta atgaagggat ttcttgttta tatgtttgta attggttata cgcgtctgaa
    13561 atacctacgg cttcatccgt agcattcttt aattcaaata acacaatagg caatccattg
    13621 acaaagagaa taatatccgt tctttttcgt tgattatcct cttccacaat aaattggttg
    13681 acggctaaaa aatcattgtt tcttggctca ttaaaatcaa ataataaaat gcgttttgta
    13741 acattgcgat taccttctcg tgtaggaatt tcaataccat ctgtaatgta tcgatggaaa
    13801 gtctgattat tgactgccat atcgggatgt ttatggacag taattgtctt aatagcttca
    13861 tcaatcactg ccattgatac ttcaggatta attgttacaa gagcttcttt caaacggtct
    13921 atcaaaacaa cttctttgta atctttccgt ttagacatcc cactatcttc taattctttg
    13981 gcagaaacag aagtatatcc cacttcttct aaccaacgca aagcaactaa ttctaactca
    14041 ttttcatcga accggtattc ttcagccaaa aattacaccc cctcatcttc tgttagttct
    14101 atttcaccag ataatagttt tggtaataat gaatctctaa tttctgttag actgtttatc
    14161 tctttactta aattaataat tttatcggtt aaaccaatca taacactatt aaatttgttt
    14221 aaatcttttt tattaggtaa aagtaccttg tgtttaagaa gatgagtaac ttttatactt
    14281 ggctgcacac tcccaatatt ataagtaatc aaattggact tattatataa taaaaattgg
    14341 tataaatgat attctaagtg ttttttagct cttaaaccaa caacattttg agcaattgtt
    14401 cctttatcag tcaaaaacat ttttatttca cctatactcc caactgtcga tatcaaaata
    14461 tcatttattt tgggatgacc acttctaaac cacgaactat aggtttcctc actaacatat
    14521 ttttgagcct tgttataatt gacaactcta ccgttaccac ttaaagtctt tacatcaata
    14581 ataggaaaat ctgttttatc tttttctaaa ggtggcgttt ttcctctatt atcaaccaca
    14641 tcaactatac tttttaattc agttatttcc catccttcag gaatttcacc taattcacta
    14701 tcaaccatct ttccaccact agatttatat ggatttccat tctcatcagg aaattcaaaa
    14761 tcaacaaacc atcgtttgaa caatgtagag gctagttctt caagattaga aataatttga
    14821 ttgtttaatt ctatttttga gtccaatgag gaaagtattt tagaaacaag taattgatga
    14881 ttttcactcg gtaatttaaa tgtataattt ctcatggttt ttaatgatac attttttatt
    14941 gtagttcctg tataatgttg ctctatatat tttttgaaat ctggagacat aatcacgtac
    15001 tttatataat ctttattcac attttctata acattaaaat aacaagcact tttccctaac
    15061 attacttttt catttttata tacagccaca ttaccaagtg ttccattaat tgagactagt
    15121 attgtcctat catttaaatc tttcttatat ttatgatatt cttctctgct taccttttta
    15181 gtattttctt taaacacaat tttcccgtcg tctaaattgt tgccattaat aaaatagtac
    15241 tcaccattat tatcatatat cggagtaccg tgtaatccat ctcctaattt atcgactaca
    15301 tcttgtagct taattatttc atatttcata tccaatccct ccaagtgctt ttcggatatc
    15361 ttcttctaat tgatgagagt gaataaattg ttctgataac aatgcagtta atcgttccat
    15421 tttttcttca aatggctcag aatcctctac ttcatcttcc aaccctacat agcgaccagg
    15481 cgtcaaaata tagtcttgtt ctttaacaat ttccaaggaa gctgatgaac aaaagcctgc
    15541 tacatcagta tattctttat cattggttcc cttccatgcg tgataagcat ctgccaattt
    15601 ttttatatct tcttcgctaa actcttttgt tgttctatca gccataaatc cttcatttct
    15661 ggcatcaata aataaaattt caccttgatg attgcgttca ttttttttag ctttggattt
    15721 tgttacaaac cacaaacatg ctggaatttg agtgttataa aacatttggc ttggtaaagc
    15781 cacaatacat tctactaaat cattttcaat tagatttttg cggatttcaa attcatcttt
    15841 tgtagatgtg gacatacttc cattggctaa cacaaatcct gctgttcctt caggagctaa
    15901 tttagacacc atatgttgaa tccaagcata gtttgcattg ttttttggtg gtgtaccata
    15961 cttccaccga gaatcctcta atagcttttc ttgaccccaa tcgcttatat taaagggagg
    16021 gttagctaaa atatagtccg ctcttaaacc tttgtgtaag tcgttttgaa atgtatcagc
    16081 attatgggtg ccgatatttc catcgatacc tctaatagcc aagttcattt tacataattg
    16141 ccatgttgtt ggattaaatt cttgtccata aacggaaata tcgccaattt tcccttgatg
    16201 cttttctaca aacttttctg attgaataaa cataccacct gaaccacaac atgggtcata
    16261 aatacgtcct ttataaggtt ctagcatttc tactaatagc gaaacgaccg aacgtggtgt
    16321 gtaaaattcc cctccccctt ttccttccgc acttgcaaac tttccaagaa aatattcgta
    16381 tgttcttcct aaagtgtcag ttttgcggtt ttctgaatct cctactttaa tattagtaat
    16441 taaatcgacc gtttctccta aacgagtttt atccaattga ggtcttgaat aatttttagg
    16501 taatacattt ttcaaagatg gattctcttt ttcaatcgca atcatcgctt catcaatcgt
    16561 ttcaccaatt tttggtgttt tagcatcacg attgattttt tcccatcttg cttcagccgg
    16621 tacccaaaaa atattatcag ccaaatattc atctttatct tccggatccg catactcatc
    16681 tttggttagt tcttcatact tttcttcaaa actgtctgaa acatatttta aaaatattaa
    16741 tcctaaaacg acatttttat attctgaagc atccatgctt cctcttagtt tatctgcagc
    16801 tgcccacagt ttatcttcaa ttttaagttc tgctgagacc attatttact tcctcctaat
    16861 cattgtcata ctttactatt ataacagctt ttaagcattt ttaggacagc aaaaaaaccc
    16921 attcaaaata gagtgggttt atccgtttga aatttttgag acatatgata atgcaaagca
    16981 attgctctag aattctcctc tgtgtaaact gaaaaatcat agttttcctt tagctcatcc
    17041 atcaatgctt gtaatttctc attatactgt tttggataat aattcttttt cctcaaatga
    17101 tggaaatctt tttcgatttc tacgtaagta cgttttgtat tcatttttaa tgcatcatcc
    17161 ttatttagct taatcaatta tatctataaa taccaataaa tcaactttat taacttgtcc
    17221 tcttatcaaa aatacgcaat agcttcaata tcatttgaat tagtttctat aataattagt
    17281 tcacttccgt cattttctaa agttttttca acaatcgttt gaacattttc tctgaatatt
    17341 tggttcccta cataatcatt cggcaatgaa tctaacgcta gtaaagagaa gatagcttgt
    17401 tgataactca aatttgattt aataattgtt actgtgtcct tcatgttatt gacctccgta
    17461 tcttttatag gtccagaaga ctaccatcct ttaaaactat agtcaatatg tgctatgtaa
    17521 aattatctgc gtatttccaa taaaatcgac ttaaaaatcg agctacttaa aagcctgtat
    17581 tattaagggt tttatgattg ttgacaaaat taataaatta ttgaaatatc aatgtttcga
    17641 ggcactattt cccaaaaaac acctctcaca ccacacaaag aaatttttgt gggtaattct
    17701 acgcttattt acatctgaga ggtatttttt atgtagcaac cagtttttga gtaaataaaa
    17761 agaccgaaaa gtcattaaaa cttgcggtct tttcaactct ttggataata tatagttata
    17821 gatatcaact ctctaaccct ttcctaaaaa atgaaagata aataattatc aaataattat
    17881 tttctcgtca gaaatttttt ctttcatggg aagtgacaat gtttttaaaa tcgttggaaa
    17941 ttgttctgtt aaaatgtcta cagcttgact aatgtctcca aaatccaaag ctccatcata
    18001 atttttagca gacaaatact ctgccacatg caatatataa aattctcctt tttctctact
    18061 atttatataa tctgcaatat cctttaaaaa tcgtttatct gaaatatctg taataacaat
    18121 tggtttctct aattccttaa gatagagatt ggatttttca cgaagttcca gttcatcact
    18181 ggttgatata ggtaagtctt cttttatgaa cattctcatt ctttctcggt aaggctcact
    18241 tacttgtaat atacactggt atttccgata aaaattctta taccattgag gaaatctttc
    18301 tcccttgtca aacttctgta aaatatcgtc taatacctca tctagttcta atattccctg
    18361 ctcaatttcc ttagcaatac ttttgggaat cgaagtgtgt ttctctaaat gatgtaaact
    18421 caaatttatc aattcaccat ttttattaga acgtaataca cactgatact taatttctct
    18481 gccacactga caaaacaagc gttctttttt agatacgtct ttatcctgat aatgcaaact
    18541 ttctttataa tcgataaatt cccaatcagt atctttcaaa tatatttggt tcaaaaaata
    18601 gttttttcta tctttcagct tgtattcaaa taagacattt ctttgcgacg ccgtcatttt
    18661 ccctaaaatt ctatctcgtt gtatttgatt aagcacaagc acttccccct ttctttatat
    18721 agcgtttaat ggtactttct gataaatgca gctgttgagc aatcgctgaa atagtatgtc
    18781 ctttttttcg aagttctatt gctctattaa ttaacttttg ctttttctct tgattcatgg
    18841 cttttgtaga tgtatacgct tctttttttc gcatattctc tttttgccgt ttttcttcta
    18901 tttttttctt ttgaacatat cgttctcgtt cttttttatt tttccttttc ttttttattt
    18961 gaggatttat catggtttta aagctatttt gttctttttc tgaaatgttt aatttttgaa
    19021 tcagataggt atttgtaaat ttatatgctt catttttaga aaatgaactt tttacaatcc
    19081 gttttatctc gctatcagat aacggattca ctagcgtggc attccagctt tcaactgtat
    19141 ccataattga tttttctgaa tactgaagtt ccattaaaag attggcatag ataaagaaaa
    19201 aatcatttct gtgacctgtc aattgataat ttctttgttt tattaactga ttcaaatcat
    19261 ggattcttgt ggtatataag ctccgtttac taaatttttt tcttactcca gaaaatgtat
    19321 cgctatcttt tttctgagat tttcttttac ttcgatagct agtttttatc ggataatcaa
    19381 ttagaatcat attatttttc ttcttttgtt tttctttatc catatcaaat tgatgttgaa
    19441 aataatctct aaagacgctt agttcatagt ctttctcatc gtctacataa atgattgata
    19501 cgtgttgatt ttcttttcct tcttttttat ttattgtgcc tgcaattcgt aagtggcggc
    19561 tacaatcaat caaataatca gctttcaact ctttaaataa acggctcaat tttgtttgaa
    19621 tcgatttata taaaaatgtc acactaggat gattcacttc aacaggagtg attatccaat
    19681 ataaatgtaa cccacgaccg ctattgacaa ctagacttgg aaaaggaaat tgttttgctt
    19741 ctaaaaaagg tattactttt tgttgccaaa agtcttcact tttcatacac catttctttt
    19801 ccgtatcaat atcgcaataa aaggcattga ccgctcgtaa attttcttct cttcttgaac
    19861 aatacctaaa gctattaatg cttaaaaaga cattttcata cacattttta tagtcgagat
    19921 tcatcaaagc ttcttctgta tttagaaaat caatactttc ataatgaata tctccttcac
    19981 tatctcttat ctcataacta atgattcgat gaaatatcat gttatatgtt aatcttctaa
    20041 cataatattc taaatctgat tctcctaatt tacgagtagg tattctttcc cccattctca
    20101 tttcatattc ttctgcaaaa ttttttatga ttactccctc catataagtt attattaact
    20161 atttatttta tttctttaga gaatacatat atgtataagt gcaggatgag aaaagatgca
    20221 cagtatcaat agttatttac attatttttt tcttaacctt tacttttttc aataatatgc
    20281 actctattag cgatggaaaa tttttgacct tattaaaatt tatccccctt ttttttatgt
    20341 ttaaaaaatc ccatcgacat ccatataata acactattat ctattctgta aatagttaat
    20401 cttaaattct atacaagaat ttttttatgg tgtatttctt gtataatcga tttataccaa
    20461 aaaaaatata ttgtcaatat ttgaaaaaac gcctaacaaa cccttttatt acaacgattt
    20521 tgtttataaa ataaaagtgt tatgagatat acaatagttt tgttcacagc taaataaaac
    20581 aaattaacac aagtcaaaaa caccatcttc agttgactaa ctccaaaaat aaaataacga
    20641 taaaaaattt atatttacac ttttcaaagt gtttttgtaa aaccatgctt ttttatcgac
    20701 tttaaacatt cttaacaatt tcttaaaaca ttcgaaatta actgtttaag cggtgcatca
    20761 cccataaata tttcttcgta ttgtctgaga ggtatttttc caaaaataaa tcgttaaaat
    20821 gttgatgttt caacatccta atgtttttgt caataaagag aaactagttg attttattgg
    20881 tttttttcaa tgccgatttt taagtggttt ctatcataaa tgttgagata aatttttgaa
    20941 taaaaaagag gcacgatttt actcacacct cttccagcaa ttccctattc actctttcca
    21001 tatatttatt ccataatgcc atgactaatt cttctgacaa aagtaatttt tcttgttcca
    21061 ttcctgctaa ttcttcaaat tctttatata gcacatttgc caactgattc actacaattt
    21121 taggaattgt tgcatttgat tgataatacg tttttttagt cttgtgttga acaaatttct
    21181 ttttggctaa ttcatccatt aattttcaat actcctaggc taccatttac ttcttctcgg
    21241 tagctggcta gattgtcatt ggttagcggt gatatataaa tatattctga ttcttccaac
    21301 acatttcctt tttcattaat aatgctatat ttcccacctt cagattcttt cattgctgat
    21361 aatccattgt cgttatattg ctgaatagta tgggcaaatg gttctttgat aatttcacca
    21421 gtatttacgt ttattagtcc atacttgtcc tctttttcta gtttataagg agcaattcca
    21481 ttgatgttcg gtttcttaat ttcaaaggca aacggttctt ttattactgt tccatcggat
    21541 gaaataatcc ctttttttct tggtgtaaaa tcaatacttg aaggagtaga atcatcaggt
    21601 gttacttttt catttaaatc ttcaaaaata tagttttctg cttcaccaat gtctcctaaa
    21661 aattcaccgt caccaattgg tgtttcaatt ccatagtcag taaatttgat aatattaata
    21721 tttgatggat aattgtaact cgtatcagaa tccaaatcat aaaatgtcca aatcgcaggg
    21781 tcatcaacta aaccacttgt atactgcctg ataaatagac ggtttttatc gtttatattt
    21841 gttgctgtat ctaatttcac tgtattgatt gttttagtag caatgttttt tacaccacta
    21901 ctgttatcac cactatcttt taatttagca tcataaatct gaacagaagt atcattaact
    21961 gtttttttat cattcatttg gtcaagaagt gtgaaatagc catttttatt tatatctccc
    22021 gacatacttc catgtgctgt tgaaaacctc ttttctctgt atccgttagc taaataatta
    22081 attgtaactc ctgaccaatc acgataagga ataatttcat atttcccaga agttaatgta
    22141 tttattgaca catcattatc aatcgttcgc tcccctagac taatctttgc taattcagca
    22201 ttttcatcta aataagtgga atatacttca tcttcatgaa catcctgttc tattactgct
    22261 gttccattta ccttttttga ataacctgtt tctaacgaat gagctagttc atctcttttc
    22321 atatcagcat ttaaaataga atttttttca tttaattccg tatcataaat cgtccaatta
    22381 tacgaactat cgtctccagt ttgtgcaata actaaaccat tttgaacata aatagcatca
    22441 taatctgtag ctgttctttc tttaataatt gattcttttt ccttaatttg ttctgtcgat
    22501 tcaagcgttg acgattggga acttccttca ctttgttgaa tgtcttgttt tttatcacaa
    22561 gcacttaaca caacaagtat taaaaagctc acacttgtta gttttatcca tttattcgtt
    22621 ttaatcacca cacctatctt attatttact tactttcaca ccaataaata gagctactgc
    22681 gataaaacca aataaaaaaa ttggatggct catcaaaaaa ttgaaaccta ataccaaaat
    22741 cccaatgata atagcaaatc cgataagcca acttatacag gctgttaaac aaccaccttc
    22801 ctgtttgtca ggaacctcta tcatttcttg aaagattttt ccacccttta atgccacaaa
    22861 atcaaccctt tctttatttt agctgcatga atgaaacgtt cgtttaagac caacaaaaaa
    22921 agctgatatt tctgaaaaga aaacatcaga gttatcaact gttctatgta tttatatttt
    22981 tttaggctct atcggtaaca aacgaacgtc tcttaccggc aattattata ccaaattaag
    23041 tagaataatg aaaagcgtta tctggtcaat tgataccgtt tttttacatt atacacggtt
    23101 ttaatacata catgacattt ttcggcaatt tctttctggg ttaaatcagt ttctgtatat
    23161 aactttttaa ttttattgac agttcttcgg tctaatccct gattacctaa tctcacgcca
    23221 ttttctttga gaattgccag tgcttctttc gtccgttccg atatataatc ggcttcaagc
    23281 tcggcaatcg ccgataatat agtaaaaata accttcccca tcggtgtcga tgtatcaata
    23341 ttttctttga tactaatcaa attgattcct ctttcttgta gctcgctcat gatattgacc
    23401 aattgcttgg tggaacgtcc taatctgtct atcttataaa taatcagcgt atctcctttt
    23461 tctaattccc tcagagcttt atctaattct tttcgattct ctttccttcc tgacacttgt
    23521 tcgataaaaa aaacatcaat tttataacga ataagtgctt ctttttgaac ttctacaccc
    23581 aaattttgtg aatttttact agttctaata taaccgattg tttttgtcat aatgattctc
    23641 ctcctttcgt ctataaattt tagtgtgcta cgctattcaa attcaaaaat tacctgacta
    23701 tcacagtcag ggtttaaaaa taatagtaca ggtgatagta ttctggattg tcttctatat
    23761 caaggttatc tttaattatt gcatcatccc ccaccgttgt cgagaaaatc acttatgtct
    23821 ttacttttat atggaaacgt tctatttaaa gttgttttat accgctttca ggaattgtta
    23881 gcagaaaaac tcttattttg tcaacaactc gaaacacaac attcaaattt tcatctttat
    23941 tcttatgatg ctatactgga gcgggaatga aggggtagat acttttaaat ttgggatact
    24001 ttcgagggat agttacgaga accaattcct ttattctatc attcatgccc tacattgccc
    24061 tttcttttta ggctccgtaa cccctacatc atccttaata aaaggctgtt ttccaaaaat
    24121 caaatgataa caaaaagata acttttggca taactttttt aaaactctat catttttgat
    24181 gctgtttttt aaaaaatcga atggaaacct ccataatttt tttaaatgcc tgccaaatgg
    24241 aaaatcttta aattccaata gcccattgct ctgcttttct gatggataaa aataaaggat
    24301 tctttcgaga atcactattt tcgtttttga aatagcaaag ctaattaatc actatataaa
    24361 aaataatgga gggagtgaac atgaacacac caattgtaaa caaaagagct attgattcag
    24421 aagaattatt tcaaataata aataatgctg aagggattta tcaatcaact ttagaaaaaa
    24481 tgcttttatg caatcgaatc agtttagcat cacgactaga cacattgaaa agacaaaaat
    24541 ggattcaaca agagagattt cacaaacaat ttgtttattc caataacttt gacttaaaga
    24601 acttaaaaac attagatgct caagcaaaca tcattcaaaa attacacaca tttaatatct
    24661 atgtagacaa gataaccgtc tttactaatt caaaaaaaca aaaagaattg tttctatcaa
    24721 cacattcgtc aggaagaact tgttttaaaa caataacaag gttaaaacaa caagctaatc
    24781 aactcattaa tcaattacca tatgagtctg ctgaatctga ttttttcgtc gaatgtgtaa
    24841 aaaatgaact aacaaaattt cctatcctcg cttcaagtat gacggaggaa tcgaaaaaca
    24901 gctactatac agcttcttta gataccatag aaattttgac agttcctact attgaattcc
    24961 ttccgtttct caaatctaaa ttagatgatt tttcttacag aaatattgag agaaataccc
    25021 attatatccg agaagatatc cttgtttaca ttgagcaaat agataagctt atttatttta
    25081 aaaagaatga acacagagaa tatgagttta atcaaataat ttccattatg gatttttact
    25141 actttttagc aaaatatttt cactcgaaag attctgtcta tttttcttta tctaaagatg
    25201 aatttgacaa tgctcacact ttgtatcgta aaagcgagga aaacaaacaa aaatttaata
    25261 ctgtccaaat aaaaaaggac aaacaaaaag cacaatctta atc
//
LOCUS       DAPBAB010000034        24271 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPBAB010000034 DAPBAB010000000
VERSION     DAPBAB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.034"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..572
                     /locus_tag="RD444_001722"
     CDS             <1..572
                     /locus_tag="RD444_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7549096.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="RD444_001723"
     CDS             576..1217
                     /locus_tag="RD444_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549097.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="RD444_001724"
     CDS             1214..2131
                     /locus_tag="RD444_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7549098.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="RD444_001725"
     CDS             2128..2799
                     /locus_tag="RD444_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549099.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="RD444_001726"
     CDS             complement(3340..3828)
                     /locus_tag="RD444_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDT7549100.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="RD444_001727"
     CDS             complement(4029..4295)
                     /locus_tag="RD444_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDT7549101.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="RD444_001728"
     CDS             complement(4295..4558)
                     /locus_tag="RD444_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7549102.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="RD444_001729"
     CDS             5034..5219
                     /locus_tag="RD444_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7549103.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="RD444_001730"
     CDS             complement(5361..7184)
                     /locus_tag="RD444_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDT7549104.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="RD444_001731"
     CDS             complement(7782..10487)
                     /locus_tag="RD444_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDT7549105.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="RD444_001732"
     CDS             complement(10793..11740)
                     /locus_tag="RD444_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDT7549106.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="RD444_001733"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="RD444_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDT7549107.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="RD444_001734"
     CDS             complement(12375..12737)
                     /locus_tag="RD444_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7549108.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="RD444_001735"
     CDS             complement(12748..13869)
                     /locus_tag="RD444_001735"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDT7549109.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="RD444_001736"
     CDS             complement(13885..14235)
                     /locus_tag="RD444_001736"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDT7549110.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="RD444_001737"
     CDS             complement(14365..15876)
                     /locus_tag="RD444_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7549111.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="RD444_001738"
     CDS             complement(16198..17550)
                     /locus_tag="RD444_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDT7549112.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="RD444_001739"
     CDS             complement(17703..18779)
                     /locus_tag="RD444_001739"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDT7549113.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="RD444_001740"
     CDS             18975..20720
                     /locus_tag="RD444_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDT7549114.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="RD444_001741"
     CDS             complement(21000..21437)
                     /locus_tag="RD444_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDT7549115.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="RD444_001742"
     CDS             complement(21421..22794)
                     /locus_tag="RD444_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549116.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="RD444_001743"
     CDS             complement(23116..23604)
                     /locus_tag="RD444_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549117.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="RD444_001744"
     CDS             complement(23626..24228)
                     /locus_tag="RD444_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7549118.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAPBAB010000035        23828 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPBAB010000035 DAPBAB010000000
VERSION     DAPBAB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.035"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(53..787)
                     /locus_tag="RD444_001745"
     CDS             complement(53..787)
                     /locus_tag="RD444_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDT7549119.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="RD444_001746"
     CDS             1114..1524
                     /locus_tag="RD444_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDT7549120.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="RD444_001747"
     CDS             1517..2218
                     /locus_tag="RD444_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDT7549121.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="RD444_001748"
     CDS             2218..3804
                     /locus_tag="RD444_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549122.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="RD444_001749"
     CDS             3823..4044
                     /locus_tag="RD444_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDT7549123.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="RD444_001750"
     CDS             4041..4802
                     /locus_tag="RD444_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDT7549124.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="RD444_001751"
     CDS             5182..5691
                     /locus_tag="RD444_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDT7549125.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="RD444_001752"
     CDS             complement(5766..6305)
                     /locus_tag="RD444_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDT7549126.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="RD444_001753"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="RD444_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDT7549127.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="RD444_001754"
     CDS             7318..8169
                     /locus_tag="RD444_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDT7549128.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="RD444_001755"
     CDS             8197..8832
                     /locus_tag="RD444_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDT7549129.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="RD444_001756"
     CDS             8852..9625
                     /locus_tag="RD444_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7549130.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="RD444_001757"
     CDS             10033..10830
                     /locus_tag="RD444_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDT7549131.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="RD444_001758"
     CDS             10808..11221
                     /locus_tag="RD444_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7549132.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="RD444_001759"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="RD444_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDT7549133.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="RD444_001760"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="RD444_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDT7549134.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="RD444_001761"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="RD444_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDT7549135.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="RD444_001762"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="RD444_001762"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDT7549136.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="RD444_001763"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="RD444_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDT7549137.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="RD444_001764"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="RD444_001764"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDT7549138.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="RD444_001765"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="RD444_001765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDT7549139.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="RD444_001766"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="RD444_001766"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDT7549140.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="RD444_001767"
     CDS             21905..22483
                     /locus_tag="RD444_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7549141.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="RD444_001768"
     CDS             22535..23017
                     /locus_tag="RD444_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDT7549142.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="RD444_001769"
     CDS             23134..23508
                     /locus_tag="RD444_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549143.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DAPBAB010000036        23672 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPBAB010000036 DAPBAB010000000
VERSION     DAPBAB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.036"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            287..694
                     /locus_tag="RD444_001770"
     CDS             287..694
                     /locus_tag="RD444_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549144.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="RD444_001771"
     CDS             708..1109
                     /locus_tag="RD444_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549145.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="RD444_001772"
     CDS             1111..1482
                     /locus_tag="RD444_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549146.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="RD444_001773"
     CDS             complement(1518..1820)
                     /locus_tag="RD444_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549147.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="RD444_001774"
     CDS             complement(2069..2269)
                     /locus_tag="RD444_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7549148.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="RD444_001775"
     CDS             complement(2574..3806)
                     /locus_tag="RD444_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDT7549149.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4060..4629
                     /locus_tag="RD444_001776"
     CDS             4060..4629
                     /locus_tag="RD444_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7549150.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4807..5247)
                     /locus_tag="RD444_001777"
     CDS             complement(4807..5247)
                     /locus_tag="RD444_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDT7549151.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5405..6169
                     /gene="map"
                     /locus_tag="RD444_001778"
     CDS             5405..6169
                     /gene="map"
                     /locus_tag="RD444_001778"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDT7549152.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6201..7124
                     /locus_tag="RD444_001779"
     CDS             6201..7124
                     /locus_tag="RD444_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDT7549153.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7200..8339
                     /locus_tag="RD444_001780"
     CDS             7200..8339
                     /locus_tag="RD444_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDT7549154.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8332..9132
                     /locus_tag="RD444_001781"
     CDS             8332..9132
                     /locus_tag="RD444_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7549155.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9132..9959
                     /locus_tag="RD444_001782"
     CDS             9132..9959
                     /locus_tag="RD444_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7549156.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9937..10671
                     /locus_tag="RD444_001783"
     CDS             9937..10671
                     /locus_tag="RD444_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7549157.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10771..11637
                     /gene="rfbA"
                     /locus_tag="RD444_001784"
     CDS             10771..11637
                     /gene="rfbA"
                     /locus_tag="RD444_001784"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDT7549158.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11651..12223
                     /gene="rfbC"
                     /locus_tag="RD444_001785"
     CDS             11651..12223
                     /gene="rfbC"
                     /locus_tag="RD444_001785"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDT7549159.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12245..13273
                     /gene="rfbB"
                     /locus_tag="RD444_001786"
     CDS             12245..13273
                     /gene="rfbB"
                     /locus_tag="RD444_001786"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDT7549160.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13371..14222
                     /gene="rfbD"
                     /locus_tag="RD444_001787"
     CDS             13371..14222
                     /gene="rfbD"
                     /locus_tag="RD444_001787"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDT7549161.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14257..16290
                     /locus_tag="RD444_001788"
     CDS             14257..16290
                     /locus_tag="RD444_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549162.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16334..17614
                     /locus_tag="RD444_001789"
     CDS             16334..17614
                     /locus_tag="RD444_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDT7549163.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17824..18630
                     /locus_tag="RD444_001790"
     CDS             17824..18630
                     /locus_tag="RD444_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549164.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18642..19862
                     /locus_tag="RD444_001791"
     CDS             18642..19862
                     /locus_tag="RD444_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549165.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19852..21438
                     /locus_tag="RD444_001792"
     CDS             19852..21438
                     /locus_tag="RD444_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7549166.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21477..23615
                     /locus_tag="RD444_001793"
     CDS             21477..23615
                     /locus_tag="RD444_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7549167.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7568 a         3941 c         4851 g         7312 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga
     3901 ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg
     3961 aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat
     4021 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt
     4081 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     4141 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     4201 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     4261 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     4321 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     4381 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     4441 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     4501 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     4561 attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa
     4621 atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat
     4681 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     4741 gaatgcaatg aataaaagag ccatccagat tgaataaatc tggatggctc ttttattgca
     4801 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     4861 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     4921 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     4981 tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc
     5041 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     5101 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     5161 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     5221 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     5281 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
     5341 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     5401 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     5461 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     5521 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     5581 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     5641 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     5701 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     5761 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     5821 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     5881 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     5941 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     6001 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     6061 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     6121 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     6181 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     6241 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     6301 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     6361 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     6421 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     6481 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     6541 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     6601 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     6661 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     6721 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     6781 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     6841 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     6901 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     6961 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     7021 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     7081 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     7141 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     7201 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     7261 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     7321 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     7381 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     7441 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     7501 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     7561 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     7621 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     7681 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     7741 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     7801 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     7861 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     7921 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     7981 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     8041 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     8101 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     8161 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     8221 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     8281 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     8341 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     8401 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     8461 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     8521 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     8581 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     8641 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     8701 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     8761 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     8821 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     8881 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     8941 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     9001 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     9061 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     9121 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     9181 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     9241 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     9301 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     9361 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     9421 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     9481 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     9541 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     9601 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     9661 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     9721 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     9781 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     9841 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     9901 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     9961 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
    10021 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
    10081 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
    10141 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
    10201 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
    10261 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
    10321 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
    10381 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
    10441 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
    10501 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
    10561 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
    10621 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
    10681 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
    10741 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
    10801 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
    10861 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
    10921 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
    10981 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
    11041 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
    11101 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
    11161 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
    11221 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
    11281 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
    11341 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
    11401 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
    11461 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
    11521 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
    11581 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
    11641 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
    11701 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
    11761 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    11821 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    11881 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    11941 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    12001 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    12061 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    12121 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    12181 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    12241 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    12301 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    12361 aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    12421 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    12481 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    12541 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    12601 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    12661 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    12721 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    12781 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    12841 tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    12901 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    12961 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    13021 cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa
    13081 cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct
    13141 aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    13201 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    13261 aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag
    13321 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat
    13381 taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc
    13441 tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg
    13501 cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag
    13561 ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa
    13621 atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg
    13681 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    13741 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    13801 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    13861 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    13921 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    13981 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa
    14041 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    14101 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca
    14161 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    14221 aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
    14281 cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt
    14341 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
    14401 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
    14461 actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca
    14521 cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta
    14581 aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga
    14641 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
    14701 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
    14761 aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
    14821 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct
    14881 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
    14941 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
    15001 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
    15061 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
    15121 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
    15181 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
    15241 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
    15301 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
    15361 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
    15421 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
    15481 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
    15541 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
    15601 gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt
    15661 gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg
    15721 agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat
    15781 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
    15841 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
    15901 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
    15961 aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
    16021 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
    16081 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
    16141 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
    16201 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
    16261 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
    16321 agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
    16381 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg
    16441 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
    16501 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
    16561 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
    16621 tggagactta ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg
    16681 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
    16741 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
    16801 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
    16861 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg
    16921 gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt
    16981 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
    17041 tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc
    17101 ggctgtctct gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta
    17161 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
    17221 gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
    17281 attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt
    17341 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
    17401 gcagtcattt atggaggata cattgacttc atacccatgg gttccgatta tttatatcct
    17461 gttggctatg gccgttcatc gatatgatga accagaagtt acggaggaat tctatgttat
    17521 ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag
    17581 cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt
    17641 gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga
    17701 aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt
    17761 accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca
    17821 caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag aaaattactg
    17881 ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta
    17941 tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga
    18001 ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg
    18061 attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat
    18121 acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta
    18181 tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat
    18241 ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct
    18301 ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg
    18361 caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct
    18421 aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt
    18481 caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact
    18541 ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga
    18601 ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta
    18661 gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg
    18721 aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga
    18781 atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat
    18841 ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg
    18901 aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct
    18961 atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa
    19021 tggtcgatga tatcattgaa tttgctgaca tcggcgacca tatttatcaa cctgtaaaaa
    19081 cctattcaag tgggatgttc gttcgtctag cttttgctgt agcgatcaat gtgaatcctg
    19141 atattttgat tgtggatgaa gcattggcag taggagatct tgaatttcaa ctaaaatgta
    19201 tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata
    19261 ttagttcgat tcgccgtttt tgtgatcgtt cctattggtt gaaaaatgga aaagttgtcg
    19321 aatatggtga tacgatggaa gtaacgacga attacgaaaa ctttttgaag aaaaaatcag
    19381 ttaaaacagt agatcgaaat aaaaaccaag tagaacgctt cgcagctcca gatatcgtag
    19441 acgtgacgaa agccgaattg ttggataaag ataaaaatcc agtggatatg ctggaacaag
    19501 gagaaaaact atacgttaaa gtaacctatg aagtaaaaga cgatacaatc aaaaatccag
    19561 ttttaggaat tgctctgcgt acagtcgaca atttttatgt gtgcggactc aatactctac
    19621 tagatggtat caagatccct tggaaaaaag gtgagaatat cttttatctg gagtatgaaa
    19681 agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa
    19741 cggttccttt ggtatacaag acaaaataca tgaacttgtt tgtcagcggt tcttatatcg
    19801 gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat
    19861 gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa
    19921 ccaggaagta aagttttaga gtttggtcct ggaaacggcc gattaacgaa acatttgatc
    19981 ggagcaaaac aatgtgaagt gagcattgtc gagctagata aggaattgtt cgattttgtc
    20041 agcgagtttg cccaagatgg attttatgga gatatcgaaa gttttgaatg ggcaaactat
    20101 tatgctggac aaacatttga ttacgttttg tttgccgatg ttttagaaca tttggtagat
    20161 cctggaaaaa cgttgaagaa agttagagaa tttttgaatg aagaaggaga aattctaatt
    20221 acattcccta accttgcgca taattctgtc atgattgatt tgttcaataa tcaattgcca
    20281 tgggcttctt atggtttgct agatgaaacc cataattctt tttatactca tgatggcttc
    20341 caaaaagttt ttgaaaaagc aggattatac atcaatattg aagattattt gtacttagct
    20401 gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt acgttatgat
    20461 ttcaaaatgc gaccatttgg cgaagtctac caatacttct tctcattgat gaaacatcca
    20521 gtagcacaaa gttccattgc gcagccgcaa aattctaatt atgtgaaagt attagaagta
    20581 atccagcaaa cgaaacaaga tgaaataatt caacaaatac cattcaataa tttcacaggg
    20641 gaaaatgaga cactaagttt ccatgtatca gaattagcag aacgaatggt gtttcgcttc
    20701 gcgcagcaac ctagctttat cgaattcagc gcagaagctg ctggagaaaa actcacattt
    20761 atcgattcaa atgctgtagt caaaacagta aatgattgtt atttgtttga tggtaaagat
    20821 ttaccagagt tctttttaac ggatattgca gggaaagaag tgactatcca ttgtcactat
    20881 cgttttatcg gagaattgac accaacaatg aaagaattgc tggaaacaat ccgtccaatg
    20941 gctgaagtta caaagcagct ttctgagaaa aatgatgaat tagaaagaga aaatcattat
    21001 cttattgaga ataatacaag attagatcag acgttgaaaa caaccataaa tcgctattgc
    21061 acgttgttgg aaagtgatga atggacaatc aaacatagat tagggcgacg taagaaagaa
    21121 acatcgaaaa aaatccaaga aaaagagctt tctctatgta ttgacgaaaa aatctgggat
    21181 gcagaaacaa agatattgaa aatcatcggg tggggaatcg ctaattcgga tcgccaacca
    21241 ttgtcctata agttatcagc cgaccaatca ccattttttg aagcaattcc tgttttacga
    21301 gaagaagtca atcaagcaaa acagctagct gaaggagcaa aagcaggatt tgaactgcgg
    21361 atcctttgcg agaaggaacg ttcattctta gttgaagcag tggctcaaaa tggtcaatca
    21421 tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc
    21481 aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt
    21541 tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag
    21601 aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat
    21661 ataatcttga tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag
    21721 gaaaagcaat cttagacttc caaacagcaa gacatcaaaa tggcatagct gtgaaattaa
    21781 acggtaaata tccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac
    21841 tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcaatc cgccgggtca
    21901 aactggaatt gaaaccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac
    21961 ctgatctgaa agagcaaagg aaagaagtac aaggatttgc ctaccgccca ttgatttcca
    22021 tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac
    22081 ttgatcaaac gtatgatcac tgggaactat gtatcagtga tgatgcttct actgatccag
    22141 ctatccgaaa gtgtctagaa tcctatcaag caaaagatga tagaattaaa gtcgttttcc
    22201 gacaagaaaa tggacacatc agtctggcaa ctaattctgc attgaaaatg gcagaaggag
    22261 agtttatagc gctcttagat aatgatgacg aattacctcc atttgcctta tatgaagtgg
    22321 caaaagtgct aaacgtacat ccagaactgg atttgatcta cagtgacgaa gataaaatcg
    22381 atgcagatgg gaatcgcttt gacccgcact tcaaagcaga ctggtctccc gatacattga
    22441 tgggaaataa ttatatctct cacttaggcg tatatcggac aagcattgtt aaggaacttg
    22501 gcggtttccg taaaggatat gaaggctctc aagattatga tttggttctt cgtgtgacag
    22561 aacaaattcc tgcagaacac atttaccaca ttgaccgtgt cttgtatcat tggcggacga
    22621 tcccgggatc aactgcaagc aatggagaag ctaaaagcta catctatgat tctggagtga
    22681 aagcattgac agatgctcta tctagaagaa atatcaaggg aagcgtacat cctggcagaa
    22741 tttctggttt ttatgaaatc acttatgatg ttttgcaaga agacttggtc agtgtgatca
    22801 ttccaactaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa
    22861 cgtcttatcc aaattatgaa atcattattg cagataatgg aagtacagat cctaaaatgc
    22921 aagaattatt tgcagaatac aagcatcagc tgaaagaccg atttatcgtc gaattaattg
    22981 acattccatt caattattca cgtatcaaca atctggcagc agaaaaagca aacggcaaat
    23041 actttttatt cttgaataac gatacagaag tgattgaacc tgactggatg acagcgatgg
    23101 tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaattattt tatccggatg
    23161 atacaacgca gcatgcaggt gtattattag ggatcggtgg cgttgcagga catgcattga
    23221 ataactatga tcgaacacat tgtggttact ttggccgatt ggtcattgac gtcaattact
    23281 tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct gtcaatggat
    23341 ttgatgaaat attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac
    23401 tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa
    23461 gaggttatga agatacacca gaaaaacaaa gacgttttgc tggggaaatc aaaaaaatgc
    23521 aagataagtg gccttcttat attgctcatg acccatttta taacgataat ctaactaaag
    23581 aaggaatcgg cgatttttcg ttgagacctg attgattaaa agaactgtat tttcattttt
    23641 attaatgaaa atacagttct tttaatttaa tt
//
LOCUS       DAPBAB010000037        23570 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPBAB010000037 DAPBAB010000000
VERSION     DAPBAB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.037"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD444_001794"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD444_001794"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDT7549168.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="RD444_001795"
     CDS             complement(1172..1867)
                     /locus_tag="RD444_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDT7549169.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="RD444_001796"
     CDS             complement(1880..2596)
                     /locus_tag="RD444_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDT7549170.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="RD444_001797"
     CDS             2926..3531
                     /locus_tag="RD444_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDT7549171.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="RD444_001798"
     CDS             3856..4179
                     /locus_tag="RD444_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDT7549172.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="RD444_001799"
     CDS             4361..5731
                     /locus_tag="RD444_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDT7549173.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="RD444_001800"
     CDS             complement(5861..7162)
                     /locus_tag="RD444_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDT7549174.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="RD444_001801"
     CDS             complement(7392..9350)
                     /locus_tag="RD444_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDT7549175.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="RD444_001802"
     CDS             complement(9527..9793)
                     /locus_tag="RD444_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7549176.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="RD444_001803"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="RD444_001803"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDT7549177.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="RD444_001804"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="RD444_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDT7549178.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="RD444_001805"
     CDS             13137..13700
                     /locus_tag="RD444_001805"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDT7549179.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="RD444_001806"
     CDS             complement(13953..14474)
                     /locus_tag="RD444_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDT7549180.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="RD444_001807"
     CDS             complement(14477..15319)
                     /locus_tag="RD444_001807"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDT7549181.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="RD444_001808"
     CDS             complement(15312..16037)
                     /locus_tag="RD444_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDT7549182.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="RD444_001809"
     CDS             16213..16509
                     /locus_tag="RD444_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549183.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="RD444_001810"
     CDS             17077..18669
                     /locus_tag="RD444_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7549184.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="RD444_001811"
     CDS             18682..19422
                     /locus_tag="RD444_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7549185.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="RD444_001812"
     CDS             19590..20939
                     /locus_tag="RD444_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7549186.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="RD444_001813"
     CDS             complement(21067..21657)
                     /locus_tag="RD444_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDT7549187.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="RD444_001814"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="RD444_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDT7549188.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6749 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa
//
LOCUS       DAPBAB010000038        22303 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPBAB010000038 DAPBAB010000000
VERSION     DAPBAB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.038"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(48..1343)
                     /gene="purB"
                     /locus_tag="RD444_001815"
     CDS             complement(48..1343)
                     /gene="purB"
                     /locus_tag="RD444_001815"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDT7549189.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1356..2480)
                     /gene="purK"
                     /locus_tag="RD444_001816"
     CDS             complement(1356..2480)
                     /gene="purK"
                     /locus_tag="RD444_001816"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDT7549190.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2473..2961)
                     /gene="purE"
                     /locus_tag="RD444_001817"
     CDS             complement(2473..2961)
                     /gene="purE"
                     /locus_tag="RD444_001817"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDT7549191.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3377..4684)
                     /locus_tag="RD444_001818"
     CDS             complement(3377..4684)
                     /locus_tag="RD444_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDT7549192.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4687..5268)
                     /locus_tag="RD444_001819"
     CDS             complement(4687..5268)
                     /locus_tag="RD444_001819"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDT7549193.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5384..5481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5840..7132)
                     /locus_tag="RD444_001820"
     CDS             complement(5840..7132)
                     /locus_tag="RD444_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7549194.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7129..7752)
                     /locus_tag="RD444_001821"
     CDS             complement(7129..7752)
                     /locus_tag="RD444_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549195.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7755..8591)
                     /locus_tag="RD444_001822"
     CDS             complement(7755..8591)
                     /locus_tag="RD444_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7549196.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8608..9495)
                     /locus_tag="RD444_001823"
     CDS             complement(8608..9495)
                     /locus_tag="RD444_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7549197.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9511..10794)
                     /locus_tag="RD444_001824"
     CDS             complement(9511..10794)
                     /locus_tag="RD444_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7549198.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11477..12160)
                     /locus_tag="RD444_001825"
     CDS             complement(11477..12160)
                     /locus_tag="RD444_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDT7549199.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12178..12795)
                     /locus_tag="RD444_001826"
     CDS             complement(12178..12795)
                     /locus_tag="RD444_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7549200.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12805..13629)
                     /locus_tag="RD444_001827"
     CDS             complement(12805..13629)
                     /locus_tag="RD444_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDT7549201.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13622..14182)
                     /locus_tag="RD444_001828"
     CDS             complement(13622..14182)
                     /locus_tag="RD444_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549202.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14356..15312
                     /locus_tag="RD444_001829"
     CDS             14356..15312
                     /locus_tag="RD444_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7549203.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMIHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15617..16573)
                     /locus_tag="RD444_001830"
     CDS             complement(15617..16573)
                     /locus_tag="RD444_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDT7549204.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCERLLTFIRESQEDAREHQENYWLRQPLYYETCFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILARIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16678..17280
                     /locus_tag="RD444_001831"
     CDS             16678..17280
                     /locus_tag="RD444_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7549205.1"
                     /translation="MQFGRFRLGMRTIKSAIAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17368..18867)
                     /locus_tag="RD444_001832"
     CDS             complement(17368..18867)
                     /locus_tag="RD444_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDT7549206.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMNEE"
     gene            complement(18883..19407)
                     /locus_tag="RD444_001833"
     CDS             complement(18883..19407)
                     /locus_tag="RD444_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDT7549207.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19582..21231
                     /locus_tag="RD444_001834"
     CDS             19582..21231
                     /locus_tag="RD444_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDT7549208.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYNQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKISDNLYDVLFKE
                     TIEHQTTVRTQSMYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGTKQ
                     YINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVPAI
                     AGFLLGKINEAKEQSVEMLLETDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQKHE
                     RNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLIHS
                     IVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21200..21916
                     /locus_tag="RD444_001835"
     CDS             21200..21916
                     /locus_tag="RD444_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7549209.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     LKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22018..>22303)
                     /locus_tag="RD444_001836"
     CDS             complement(22018..>22303)
                     /locus_tag="RD444_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDT7549210.1"
                     /translation="ALKSNHLAGFATDVFANETEVFFRSFKPWETIPDPAIQQLVEMY
                     PRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPKARQLR"
BASE COUNT         6567 a         4807 c         3904 g         7025 t
ORIGIN      
        1 ttatttttgc tattctattt taattgaatt ttgcaactca ctcttcttta ttccaaacct
       61 actcgtttga atatcacgtc aacgtttttc aaatgatgat gataatcaaa ggcatcgtct
      121 atttcttctt tagataatac cgatgtgatg gttggatctt cttctaacag cgggcggaac
      181 tgcacttggt tgtcccaagc gtacgcagtt tttggctgaa caagatcata agcttcttct
      241 cttgtcattc ccttatcgat caatttcagc ataacgcgct ggctataaat caaaccaaat
      301 gttgcagaca tattccgctt catgttttca ggaaagaccg ttagattttt aacgatattg
      361 ccaaaacgat ttaacatgta attcaataaa atcgtggtat caggcaagat gattcgttct
      421 gctgaagaat gagaaatatc tctttcatgc cataatgtga cattttcata ggcagtgacc
      481 atgtgtccgc gcaccactct ggctagacca gccatatttt ctgaaccgat cggattcctt
      541 ttgtgcggca tagcagaaga acctttttga cctttcgcaa aaaattcttc tacttcacgt
      601 gtttcggatt tttgcaatcc gcgtatctct gttgcaaaac gttcaatact tgtagcaata
      661 agcgccatcg ctgagaaata ttcagcatgt agatcccgag ggagaacttg agtagagatt
      721 tcttgtggac ggatccccaa ttgtttacat acatactctt caataaatgg cgggatattt
      781 gcgaacgttc ccactgctcc tgagattttt cctgcttcta ctccttttgc cgcatgttca
      841 aatcgttcga tgttccgttt catttctgaa taccatgtgg caagtttcaa tccaaaagta
      901 gttggttcag catggacacc gtgtgtccga cccatcatca ctgtatattt gtgttctttt
      961 gctttttcgc caatgatttc cgtaaaacgt ttcaagtctt cccgtaaaat atcatttgct
     1021 tgcttgatga gatagccata agcagtatct acgacatccg tacttgttag accataatgg
     1081 acccattttc tttcttctcc caatgtttca gaaaccgctc gtgtaaacgc aacgacatca
     1141 tggcgtgtta atttttcgat ttccaagatc cgatcgatat caaatgatgc atgctcacga
     1201 atttttttca catcttcttg cgggatttca ccaagttctg cccaagcttc atctgctaaa
     1261 atctcgactt cgagccacgc acggtatttg ttctcttccg tccagatatt ccccatttca
     1321 gggcgtgtat aacgttctaa catgtttttt ctcctctaat cccaaatatt cgtttgtttg
     1381 atttcttcca gcgtttgctg gatgtcttct gtcaagatag tgatatgtcc catcttgcga
     1441 cctttttttg cttgtttttt tccatagaaa tggaattgcc agtctggctt attctcaatc
     1501 aagcgatagc tttccagcag ttgttctccc aaaacattga ccattactgc attgcttatc
     1561 aaacgtactt tttctcctaa tggccaacca caaatacctc gaatatgggc atcgaactga
     1621 ctcatagaac aagcttcaat agaataatgt ccagaattgt gaggacgagg agctaattca
     1681 ttcacatata gcccgcctgt ttttgttaaa aacatttcta cccctaaaac acctgaaagg
     1741 ctcactgctt ctgcaatcac acgggcaata cgctcagcct cctcgatcac atcctgatca
     1801 atcgcggctg gagcaatcgt ttcatgaaga atattgttgt ggtggatgtt ttccacgacc
     1861 ggaaaagtcg tgaaatctcc ttgaccattt cctgccacca tgatcgaaat ctctttttca
     1921 aacgggatcc atgcttctaa aacacaggtt ccttctctta aaaggtccat ggcaggagct
     1981 agatccgccc gacttttcaa aacatactgc cccttgccat catagccgcc tcgagtggtt
     2041 ttcaaaacac aaggatagcc gatcccgtca atcgcatctt ggatatccgt cggactgacg
     2101 atcgttgcat aaggagcaat cacgatgtta ttcgtttcca aaaaagattt ttctaacagg
     2161 cggtcttgcg taatcgcaag caagtctgtc ccttgaggaa taaatgacat gggtaaaatc
     2221 gcatttaacg cctctacact gacgttttcg aattcatagg taatgacatc tgttcttctg
     2281 gcaagctctt tcagcgcaaa ggtgtcatca tagacacttt caatgtgcca atctgcaact
     2341 tgtgcggctg gacaatcgct cacaggatcc agcacaccta ctttaaagcc catttctttt
     2401 gcactaatcg ccatcatacg acctaattga ccgccaccga cgatcccgat cgttgaacca
     2461 ggcaacaaag gtttaaccaa gttgatcact actttctata acagttttag ccaacattga
     2521 acgtctttca gccaatttag cctccaattc taaatcatac atggaaagca tctgtatcgc
     2581 aagtagtcca gcattaattg ctcccgcttt tccgattgct gtagtagcta caggtactcc
     2641 tccaggcatt tggacaatcg aaagaagcga atccaaacca tttaatgttc gtgattggac
     2701 cggtacaccg ataaccggca aagttgtttt tgatgcgacc atacccggca gatgcgcggc
     2761 tccccctgct cctgcaataa tgattttgat tccttttttt cgagcgtttt gtgcatattc
     2821 aaacatcaaa tctggggttc gatgagcaga aacgaccttt ttttcataag acacaccgaa
     2881 ttcatccaat atttgacatg cttctttcat tgtttcccaa tctgaagtac tccccataat
     2941 cacagagatt tttgctgtca ttgtttttcc tcccagtttt tctacattca tattttaaca
     3001 atcacttttt ttgatgtcaa agaaatttcg aatgttaaca aagaaaaaca ttttattgtt
     3061 cgtgtttatt aaagataaaa aaaaggaatg tgacaaaagt taggtcacat tcctttttat
     3121 ggataaacgg tgttctcctg ccaactcctc ggtcacaagg aacaatattc aacaaacatg
     3181 agaagctttt ttttgaaatt gttccttgtg actcggagat gaacggcagg ctcacgacct
     3241 cttccttcaa cggtgttcaa tcagctgacc ctcggtatta gctcaaacaa acggcaaaat
     3301 acgaggaact atttttccgt ttgtttctct aacaatcagg tcaaaacgct gatttcacaa
     3361 cctttctttt gctctattac ttcttgaagg cttcttccaa tatttcttca tcttttttca
     3421 atccattaaa gatcaaattc aatatgatag ccgtaatgct actcattacg atgccatttc
     3481 cagtgaacat ttgcaatgtt tgcggcattt gactgaataa tgtcggcgta acattgaaac
     3541 ccagcccaaa tccaacagaa acagcaacaa tcaatagatt tttatcattg ctgaaatcaa
     3601 cttccaacag catacggatt ccttggactg ccaccatccc gaacatgact aacattcctc
     3661 caccaagtac cggctcaggg atgatttgtg ctaacgcacc gattttaggg aataatccta
     3721 aaacaatcaa gaagaatgca gagaaataga tgggttttct tgttttgatt cccgaaagtt
     3781 gaaccaatcc gacattttga gaaaagccag tataagggaa tgtattgaaa ataccgccta
     3841 agataacggc aagtccttct gcacgatatc cctttttcaa ttcttcttct ccaacatttt
     3901 tcccagtaat gtcaccaagt gcaaaatata cacctgttga ttctaccatg ctgaccatgg
     3961 aaatgatgat cattagtaaa atcgaccata cgtcgaaagt tggtttacca aaataaaagg
     4021 gttgtggaac atggaacaac ggcgcatctc caatcgcgct cagatcaatc attcccatga
     4081 aagcagctaa gatgcttcct ccgactaaac caatcaatac cgcgattgaa cgaatgaacc
     4141 cttttgccag catttgcacg cctacaatta ggagaatcgt cacaaatgca agtagcagat
     4201 ttgttttgtc accaaaatca gctgctgttg cattacctcc acccattttc tcgattgcta
     4261 caggaatcaa agtcaaacca ataacggtga taaccgttcc tgttactata ggagggaata
     4321 gttttttaat tttagaaaag acaccagaaa ctaacacaac aaagatccca gaagcgataa
     4381 tagatccgta gatagctcct actccttcat tggttccgat caagatcagt ggagctactg
     4441 cttggatcgc acatcctaaa acgacaggca agccaattcc gaaaaatcgg ttaactgtca
     4501 attgcaataa tgtcgctaat ccacacataa agatatcaat tgaaatcaaa taagtcatct
     4561 gttcttgatt aaagcctagt cctgtaccga tcaaaagagg caccgctact gctcctgcat
     4621 acatcgctag cagatgctgc agccctaaaa ccgcagcttt tccattttgt gtttcttttt
     4681 ccacgattat gcgtcctcct caagaaactc aacttgtcca ttcgtcaagg aagcaatacg
     4741 cgcaagagaa acgactctca atcccatttc ttcaagtaac tgacgtccat cttggaaaga
     4801 cttttcgatg acgatcccga ttccttctac ttgcgctcca gcttgctggc ataattcgat
     4861 caatcctttt gccgcttgtc cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc
     4921 tgaagataaa aatttacgag aaatagaaat cgtacttgtc acttgtttcg taaaagaata
     4981 aacagaagct gtcaataatt cttcatccat cgtcaaactt tttgcttttc tagcgaagat
     5041 cattggaaca tctaattctc ttgcagcaaa taaagctggt gcgatcccag aagcttcaat
     5101 ggtcacaact ttcgtgattc cttgttcttt aaatacttct gcaaaccgtt ttcccatttg
     5161 ttccatcaat acagggtcta cttggtgagt gacgaagctg tcgactttta atacgccctc
     5221 gcctaatata cgtccgtctt tttgaatacg ttcaactaat tctttcactt tatttgctcc
     5281 tttatcattt attaataccg aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt
     5341 aaagaaaaac accctccact ttatcataag aaaagaaggg tgaaatagcc aatcttttac
     5401 ttatagtcca gcatttacgg tactggctag aaactgtcat ccatattatg acgatatata
     5461 agtaggtatg taaaactaat aatagtgtaa attcattttc ttgcaactgt tttgcacgtg
     5521 aaggcagttt gccatatttg aataaaaaat gttgtttcaa atattgaaaa cagtctaaca
     5581 ctactttctg aaactgacaa cgtggtttac ttattttttt gtaaaatcta atgattttta
     5641 atgcattatt cctttttgtt cgggtttaaa tagttatttt tattttctcc tatctaaaaa
     5701 agcagcccac ataaaaagaa ggcatgacaa aaagcatgtc atagcccctt tttccgaata
     5761 aatagtgttc aaaagacagc ctatcggcaa taaggcaaga ttttataaac tacgggaagt
     5821 aatttccgta aatctattct tattctttta agctgacaag ctcgtcaatc actactgctt
     5881 ttcctgaagc aatagaatga tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta
     5941 cgtgagttgc tgcccgattc cacggatctt cttctttttc ttcaccaaat agatcatcta
     6001 ataaacgcgg atctcctccg ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg
     6061 gctcaccgaa catcggatag acatggatgg actgaagaac ggctgctcct tcatcctttt
     6121 tatctccacc tgcattgatg taagactttt cgatcacttt catttcgatc cgtcctttgc
     6181 tgccattgaa agaaacattg aatccttccc acggcatata cgcgtttagc gaatacgtca
     6241 aaatcgcacc agtttcatat ttgaccgtta cgcctagtgt atcttcaata gagatatcat
     6301 cggaaaatac actgcggtca cgaatatatc cactgtcttt ttctgcttct aaatacattt
     6361 ctcgcaattc ctcattggct gacaggtcta tagcaaatgg atcagcttta gctagttcgc
     6421 ttccatagca tcgactgtaa aatatttcta ctccgcgttt ctccgcatta tgcatcccat
     6481 aaaagtttaa tccaccgaat gcaaatacct tttggggacg gctgttcaac caaaaattca
     6541 ctagatcaaa atgatgggta gatttatgaa caagaagccc gccgctattg tgcttatttc
     6601 gatgccatct tctgaaataa tctgccccgt gttgcgtatt taacagccat tcgaagtgga
     6661 tagaaaacac atcaccaatc acatcacttt ctatcagttc ctttatcttc gtgtgatacg
     6721 gtgcataacg ataattaaaa gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta
     6781 ttatttcttg agctttttcc tcatcgatag tcagaggttt ttcgcaaata acatcatatc
     6841 cttttttcat tgcacgaatg atataatcat gatgcgttcg atctactgac gtaataatga
     6901 cttcagccgg ccgtacgtca tccagtagtt gctcaaattg tgatggcata catgtcttta
     6961 cttttttctg tccatacttg ttcacaagta tccgatttgc ataatccatc cttgtttgag
     7021 atacatcaca aaaaccgact agttcacaag tttgatgata cgtagaggca attgcctcat
     7081 aaaaaaaacg cgctctccca ccaagtccga cttgtacata ttttttcttc atttaaagtc
     7141 attctccttt tcttttaagc aaaatactgt gacaaatggc agccctggat acagtaatgc
     7201 agtgagtaaa aaatcatgct gtatacacca gaacagtcca tacaaaaggc ataaatacca
     7261 cagaaacccc ttgacgtttt ttgacaacag ccagatattc cttttgatca aataaacaag
     7321 actatctgct ttatcttctt tgcagatatc aattagcgta ctaaaaaaac tatgggatac
     7381 aaaaagcaac agacacaata gaaaaggaat cagataccaa gctccaatct ttcccactaa
     7441 taaataccaa tcaactgcaa aaataatgac aaacagctga aacaacgtaa tgatgaaaca
     7501 catccgtttg atttttcctt tcctccaaca tacctttttt tctgccaagt aatggacaga
     7561 tagataataa gaaggataca tcatcaaaaa ataaatcaga tagacaacac gagcagacag
     7621 cactaatcct aaagtgaaca aacatcctag aactaaagcg aaaagagcat tactgataag
     7681 actggttaaa aaaatccgac agacatagta gttgagcttg taaagaaaca tctcttctat
     7741 tggctttttc atattcagcc cttcagaccg cttgtactga tcccctggac aagatattta
     7801 ttgaagaata agaaaatcag aaaaaccggt agcagcgaca acgtagacat cgcaaacata
     7861 ctcgcgtaag tcgtactaga tccagcgtct gcaaataatt tgatggcaat cgataccgtg
     7921 taagatctcg gcttgtttaa ataaatcaat ggtcccatga agtcgtccca tttccaataa
     7981 aactggatga tgatcgtcgt gatgatcgaa ggtttcagca aaggaaaaat aattttggta
     8041 aagacactat acttgctgca tccgtcaata atcgctgatt catctaactc tttggggatg
     8101 ctgaccatga attgcatgat ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa
     8161 atcagcggca catacgtccc aaccaaattc agccgattgt aaataataaa ctgcggaatc
     8221 aaaatcactt gtgcaggaat catcatcgtt actaacatga cgacgaacca gaatcttttt
     8281 cctttgaaat cgattctcga taatgcgtaa gcaatacaag tcgagctcag aactgtccct
     8341 atcgtcgaaa ctgcagagat gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca
     8401 gcaaaccccg cccatccttt tgcgtaattt tcccattgaa aagtctctgg aatcaaattc
     8461 agcgaaccac tcataatctc ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc
     8521 ggataaatca tgactagacc taatagcaaa ataaaaccgt attgaaacca tttcgttact
     8581 gtattattca tttcttcctc ctctcattca cacaacgacc catttttttg atgatttgaa
     8641 aatcagaaag gtgacagctc ccataatcaa cagcatgatc cacgaaatag cactagcata
     8701 ccccatagaa ttaaaagaaa aagcttggtt atacacataa agagcataaa agttcgtggc
     8761 atcattcgga ccaccttggg taatgataaa tgcctgcgta aaagtcataa acgccgcgat
     8821 tgtctgcata accaagttat acaaaatgat cggcgaaaga agaggtaacg tgatcttgta
     8881 aaacgtctgc catttgttcg ctccatcgat ctctgccgct tcataatacg agcctgggat
     8941 ctctttgata cctgcagcaa agatgatcat tgccgaaccg aattgccaag cagacatcaa
     9001 aacaagaggg atcatgacca gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa
     9061 tcgttccagg aaaatattca ctaacccgtt tttagaaaac aactcccgcc aaactagagc
     9121 gatagaaata ctgccaccga tcaaactagg cacataatac aaagaacgaa agaaagtcgt
     9181 tcctttgata ttcctcgtta aaagataagc aatcaataga ccaaagatca gtttcacagg
     9241 tacagatgca aatacataga gaaaagtgac tcggatggag ttcaaaaaac gggcatcact
     9301 cagcatcttt atataattat ccaagccgat ccatgagact ggatttccca aacgataatc
     9361 ggtgaacgaa taataaagtg acaagatcat cggtatcact gttatcaata gaaatcctag
     9421 gatgaatgga gaagcaaata cgtaaccgac cacattgtct tgatccaaga accgttttag
     9481 tttttctttt ttcatgcctt cctcctgctg ctatcctaga atgtttgacg caaactcata
     9541 aagactctct gctgcttctt ctgctgttac ttcttcataa atgacttttt caactaaaag
     9601 attgtattgg tcttgaattt ccggattcaa agcactatca aagacattca cttcgccgtt
     9661 gtccatatcg gaaatcattt ctaaataatc aaatgtcgct tgcaaagttg catcatctgc
     9721 acgagcgagc tgttctcgaa tattcgacat gattggtaca ccacgttctc cttttaaaat
     9781 ttcattggct tcttcactgt tttgaaagaa atcaataaac ttagcagctt cttctttatg
     9841 ggctgagctt tttgccatac tcagcatttg cgaagaacgt aaagggatac cagattctcc
     9901 atctgctttt ctgcgaggtg gattaatcaa ctgaatctcc atatcttcag gagcccctga
     9961 caatatttct gtcaactggt tacttgatag aaacaccatt gccgcttctc tatccctaac
    10021 cgcctgaaaa gacagatccg tcgtacttgc agctccacca cctggttctg gatgggcccc
    10081 tttgtctacc agatttttac gcagctggaa aaaatctgtt agatagtctg gattttcaaa
    10141 tcccaagctg ttagatagat cttcccgatt ataaaaattc aatcctttct caacttgtgg
    10201 tatttgaata atacagccag ctataaaatc ttcgaatctt gaaatagcat agattcctaa
    10261 ttcttcagtg atttgatagc tgatttgttc aaattcatcc catgtccaat ttgaagctgg
    10321 ttcagggata tttaattgtt caaatatatt tttattgtaa gcaatcgcaa acgaattcac
    10381 cccgttagac agtccaacta attgcccgtc gtaagtcgtc gttgctaaat aggattcatc
    10441 tgcattagaa atatcaatta tcccttgctc cacatattca tctaatggtt caatctgatt
    10501 gatgtattga ggaaattgat tgttcagttg gaaaatgtcc cagatatcat ctgatgctgc
    10561 tagtgtacct aattttgtgt aatatccttc ataatcataa aattcataat cgatcgtcac
    10621 atggggattt ttttcttcat aaagctcgat tacttttact gttgcatcgt gtcggacttg
    10681 cgaaccccac caagccatgt tcagcgtgat ttttttgtca tcttcttgcc cacaagccgt
    10741 caagaagaat aacataacac ttaaagctaa gatgattctc attcttttct tcattctctg
    10801 cacctctact tccattgtta tttttcaata tactgtattt ttatacagaa catatttatt
    10861 aaacgatctt tttcaagggg aataaaaacc aagacttgcc ttatcgttct gcctaggatg
    10921 aacaactcaa tactttcaag aaaaattaga tagtgctgat tcttctttca tcgctaaaaa
    10981 cacttcttgc ttcaatgatg ggaggataat tacgcaaaga aaaaaggata tcagacaact
    11041 gcccaatatc cttgacatag ctaaataagc atgcttcttc gtgtgcgctc ttccctaaaa
    11101 aacagaaaaa taactagtaa ttctgtatga aattaggtac aatatacacg tcgatgcaca
    11161 agcattgact atggcaactt gattggtcag ttcccgctga cttttcaagc cccgacaatt
    11221 tagctgctac taaattgtcg ggtgcctttt ttagttttta ttcgtttttc attctataga
    11281 aagcgcttca ataaatcaag acattttttt gttagaatgc taataataac aaaaggtttt
    11341 aactcttctt atgtacatta caggtaaaaa aacaaaaaaa tttctctttt ttttgaattc
    11401 tgaacaaata gtactggagt acaaaagacg aggaagagcc actatctgct cttcctcgtc
    11461 ttttgattct cacctgttat tcatgattct ttttctcagt ttcccgatat ttttccattt
    11521 tacgactaaa aagctctccg ctacttggtg gcgtatatgt gatggcattt gctcctgctt
    11581 gaatggtctc agcaatactt tcttccgttg gaccacctgt cgcaatgata ggaagttcag
    11641 gatatttttt acgaaaatgg cgtactgctt ctgcagtttc tttacctgca ctgatattga
    11701 taatcgaaat accagctgct aacttttcat ctatttctgt atatttcgac gttaccgtac
    11761 tgataactgg aatatcaact actcgacaaa cttcttttac tgtctctaaa ggcgtaggac
    11821 cgttcaaaac gacccctaac gaaccttgcg cttctgcgaa catgctcatg taggaagaac
    11881 gagcaccttg tgtcaaacca ccacctacac cggagaaaac tggaatgtca gctgcctcaa
    11941 taatgctttt agtaatcgct ggatgaggtg taaaaggata cacagcaatt actgcattcg
    12001 catttgtatt acgtatgatc gcaatatccg tagtaaagat gatcgaacgg atcaattttc
    12061 caaatatctt gatcccgctt gcttctaaaa tgatttccgg tactcttacg atatcttttc
    12121 tcaattccgt catgatttct ggcgtaaatt gttctgccat cagccacttc tcctttgcta
    12181 tctccggtca tactgattca attgatacgt ctcgatgacc tggatgattt tttgcgcata
    12241 accggggtct gtcgcataac ctgcatcttg caatgcttgt gcggcttcct gatagttggt
    12301 ggccgtaatc actttttcat acagcgcagg attccaatct actccttgga cgaacagttg
    12361 tgtatggtca tccatggact gttcccatga atcatacact ttgaagtctc cttggatagt
    12421 tatccattct tcgttaacaa attcctttgt ttccaggctt actttcttct gatccccata
    12481 agccttgatc ccgaaaagat tattgtattt gctagccaag gtactttttc cccaatttga
    12541 ttctaatata gcttgtccta aaatgatact aggaagcact ccataaccat cttgtagttc
    12601 ttgggcatgc ggaacaagcc gttcaataaa ttgctgatgg gattgcgctt cattttcttg
    12661 ctgattatca gtaaaaggag aagacagatt cttcaaggaa aaaacaaagg ctaagagtat
    12721 cactgctaca ccggcaacca ctgctggcca acggatattc ttgcgctttt gctttttcca
    12781 cttttttttc gccatgatca attcctactt ttcatttaat tcattcagat attcttctag
    12841 cgtcaattgg tgtttggtga tatactcagc gttttcttta ccgacataac gtaagtgcca
    12901 aggttcatat gtgatcttag ttatatcttc gtgtcctttt ggataacgaa cgataaatcc
    12961 atactgcgca gcattatctg cgatccattt agctccaggc tgatctccgt aacttgcatc
    13021 caatacagta gaaggataac tgttgtacca gttttgatcg acgacatcga ctgccagacc
    13081 tgtgtggtgt tcgctgtatc ctggctccgt aatcgtttct ttagtaatct ttgtcgcctc
    13141 ctcttctgat acaccttgac gactcatcac atcttggaca ttttgttcga aaacttgttg
    13201 ttgggatgat acagaccggt aagctgagac catcactaat ggaaatccag ctgcttcagc
    13261 agccttggca aatgcttcat attctggagc aatgcgtttg tcgatcaagt atccgttact
    13321 taatgatgcc aattgagtag actcatcaat ttctttttct aatttgtgat ctggaccaac
    13381 caaaactagc ttccaatcgt ctgttttaac atctggcaat tgtttggacg tacgatcttt
    13441 tgtttctttg tcctttacct tgctgctggt tgtttgactg ttcttttctt tactagcagt
    13501 tgttttggtg cttgttcgtt cgactgcttg attgccggct tgaacatgcg aatcatcatc
    13561 gctaaaaata acatagccca ttgctgcgat cataatcaca actgctccaa atcctaaaaa
    13621 tttattcact tgaaaaatcc tttcgttgtt cttcacgttc cccattcgcc tcatgaacgt
    13681 aagtttcttc cagattttca ttcacccaat cgagaagttc gattttttct ccttcgatct
    13741 gtttttgaac atctgctggc agacgaaaat tcaaaatatc atcatagccc cattcatcat
    13801 attgaatact tactttcaag tcaaggtatc taccagcctg ctgattattt ttcaaaggga
    13861 tcagctcgac ttttgctgct ttgctgctaa gccatttttg cgcgatgcgt gttttgattt
    13921 gcttgtactc tgcaacggca acgtttcgtt tttcgggaaa aataatcgtt tgacgcaaaa
    13981 tcttttgcgt tagcatccat tcataatcag tgaaaagatt ttttactttc tgcatcgctt
    14041 cagatggcag atttttctcg ccatctttcc atttttgcca ttgttctgga ctgatcagca
    14101 agtattcatc taaaaaattt ttttcagttt caaactgttc taataataca ctcataataa
    14161 gatcaatata tacttgatgc acgtttatcc cctcctttta actattctac ttaagttttg
    14221 atcaaaaagc acctcttaaa tcgctgcttt catttttttt aatgtttcaa gtcctctcct
    14281 ctttattctt cgtgattttt gttataataa tagacagaca agaaactcaa tccaactatg
    14341 aaggagcgtt ttcagatgaa aaaatatcaa tggggaatcg ttggtcttgg taccatcgca
    14401 catgagtttg ccgaaagctt caatcaagag acgagcgaac taacagcagt tgcttccaga
    14461 acttcagaaa aagcggaaaa cttcgctcac cgttacaata ttccaaaagc ttacggaagc
    14521 tatcaagaaa tgttagacga tgctgaaatc gacatcgtgt acattgctgt accaaataga
    14581 cagcatatcg accatatctt ggcagcattg gaagcaggca aacacgttct atgtgaaaaa
    14641 gccatcacga tgaataaaaa agaactggct gacgctatgc gcttagcaga agaaaagaat
    14701 ttgatcttag cagaagcgat gacaatcttc aacatgccac tttatcagca gttacgctca
    14761 ataatggata caggaaaact gggggcttta aaaatgatcc aagcaccttt cggcagttac
    14821 aaagaaccag accctaaaaa ccggttcttc aatccagaat tagctggagg agcactgttg
    14881 gatatcggta cgtatgctgt atctttcgct cgtttctttt tgagctctca acctgaagta
    14941 gttgcctcta caatggttcc ttttgaaaca ggcgtggacg aacaatcagt cactatcttg
    15001 cgtaacaaag aaaatgaact tgctgctgtc agcttgactt ttcaggcaaa aatgccaaaa
    15061 gtcggtatcg ttgcctttga gaacggttat gtgacgattg ctgattaccc gcgtgctgat
    15121 cgtgcggaga tcttatttac agatggtaca aaggaattca tcgaaagcgg gaatacatct
    15181 gaagcaatga attacgaaat aaagaatatg atacacatga ttgaaggtga cttgcccaat
    15241 cgctctctct tcttgacaaa agatgtcatc gaaatcttgg atcaaatgca agttctatgg
    15301 aaaaatatgt aattttaaaa ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    15361 tcaaacaaaa atagctcctc ctatttttcg tttggctgga cttattaccg cagatgcaag
    15421 cttttgaaca ccgtttaagg aagaggtcgt gagcctgccg ttcagctccg agtcacaagg
    15481 agcaatttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
    15541 tgacaggaga acaccgttta aagaagaggt tgtgaactca gctttttgac ctgaatatta
    15601 gctgaattca caacctctat ttttggattt tttgttggga gaattctgcc ttgatttcaa
    15661 taaaactttt aaaagactgc aaaaactgga acaattctgc tcgatcctcg aattcagggc
    15721 gactagtcgg cagaggcatt tgccgataca atacataaac ttcttcaata cgtgctaaaa
    15781 tttgtctgcc atcatttgac tctgaaaatg tttctgcagt ataaatcaat aaaccataga
    15841 tatgttttcc ataataagct ggttgctgga ttctctctag attttccagc atgtctttaa
    15901 tcacattggc ctgtgctctt ctcatagaga agcaagtttc gtagtatagc ggctgtctga
    15961 gccagtagtt ttcttgatgt tctctggcat cttcttggct ttcgcgaatg aatgtcagta
    16021 aacgttcgca tttgatctgc acatcttctt tattttctga taaaagaaat tctgccattt
    16081 gtctcaaaat atttcgaaat tctttttcga tctgtatttg gttttccttc agctgcttcg
    16141 tacgatctgg catgtaaaca tttgcaagca aagcaaatac taatccaatc gccattaatc
    16201 ccgcttcatt gccgatcagc tgccaagaca tgttttcctc cactaaataa tgagtgacca
    16261 aaaccgaatt tactacgatc ccttcggtta atttgaagcg agaggcaagc gggatgaaca
    16321 ggaataaaaa aagactgaag ccaaaaatag tatacccaac agcaaaaaaa ctgaacagtg
    16381 ctgttgccgt agctaatata aaggctgcga ttcgattcat gcccgtacgc aatgtagagc
    16441 gcttggtatt tcctacactt aataatgcaa tgatccctgc agctggccaa tacaacagcg
    16501 ataagctacc cgctgtgacc atcgaaaaga agacactgat cactgttttg actgttcgca
    16561 ttcctatacg catttacttc cccctttttt tattttaatt atacttgaat aatgaaaaac
    16621 tagctatcag acaaaagttt aggtatgata gtaaaaagac caaaaaggag aatctctatg
    16681 caatttggcc gttttcgact aggtatgcgt acgatcaaaa gtgccatagc tgttttttta
    16741 tgtatcttgt tttttcattt gactcaacga ggaatcccga tgattgctgc attatctgct
    16801 gttttttctt tgcgtcagga cttgactaca accgtttctt ttggaaagtc taggatcatc
    16861 ggcaatacat taggtggtac attagctatc atttattttt ttgtcaaaag ctattttcac
    16921 aatgattttt tggtagaatt gatcttgctt cctattctag ttattattgt gatcgtcgtg
    16981 tcagatggta ttaacaataa ttccggtatc atttccgcaa tcgctacgtt gttgctgatt
    17041 gcgttgagca tccctcaagg cgaatccgct ttgtatgcta tccaacgtgt actagatact
    17101 tttataggga cattcattgc aatcggtatc aatttctttc ttcgtcctcc tgaaacagaa
    17161 aagaaggaag aactatcaga agatttggtc gaactccaaa agaaagaacg attgctaaga
    17221 acaaatttac aagagataga agctaagatc caaaaacaaa agaaaaatag cgaggactaa
    17281 ttttcttttg caataaaata aaaaaatgga gagaagaact ttaaaaagtc ttcctccatt
    17341 tttgattgaa cggtgaagtg tactatttta ttcttcattc atcgagtttt gttctgcctc
    17401 ttttggcgct tcatacatat ttacttggct agtagtccca cgtgtcgaca aattctctac
    17461 gatttccttc aagtcaagtc ctgttgtcgc ttttagtgtt tcttgagtag aagaaagcaa
    17521 atttgtggca taatttgtca ctcggttcgc tccactggtt tctcctgaac tgcttcctgt
    17581 atcgacaaca gagatttttt cgatatttcc tagcggctgt gctgcttcgc gcatcaattg
    17641 cggtagcata tcgataacca tactcaagac agctgcttcg ccatattcct tgaaggcatt
    17701 ggcgatcttt tgttttgctt cggcttcggc ttttcctttt gctagtgctg cttcggcttc
    17761 tgcttgtcca gctaaacgtg tcttattggc ttccgcttct gccagtgctt ccacacggaa
    17821 tcgttctgct tcggcttcag tcacttcttt gactttttga gcttgtgctt cttgttcttt
    17881 agcgtaccga tcggcatctg cttttttctt cacttccgaa tcatattgct tttcacgacg
    17941 tgtgatttct ttttcttcca gttcgatttg tttttgacgt tctacgattt taatctgcat
    18001 ttcctgttcg attacttgtt gttgtgctcg ggcactttct aaattgtagg cttggtctgc
    18061 ttttgcttta gcaacatctt gttcttgttt gtaggttgct aatttcagtt ctttttcttt
    18121 taatgattct gcgatctctg tttgtcgctg taattcagct gcttgtgatt ctttttctgc
    18181 ttgagctttt ttgatacgcg tttcttttag agcttccgct tctgctatat ctgcatctct
    18241 tttgacttgg gcgattcttg gtttccctaa agagtcaaga taaccgtttt tgtctctgac
    18301 ctctttgata gtgaaagaaa cgatgatcaa ccccattttt gcaaggtcta cagaagcgac
    18361 ttcttgtacg ctttgactga acttatcgcg attttgatag atttcttcta cagtcataga
    18421 accaagaatc gagcgcaagt gtccttctaa tacttctctt gcttcatttt ccagttcttc
    18481 tcttgtcttg cctaaaaact gttctgctgc tgtagcaatc tcttctacag atgaaccgat
    18541 tttgatgatc gaagtaccat cacacatcac cggtacgcct tgttccgtat acacttcagg
    18601 ggtggagacg tccaatttac tcgataatag actgatccga ttcgaacgtt gaaaaactgg
    18661 caagacgaat gcgccacctc cacgcacaat tttgattttg ttattgcttt catcaatgtg
    18721 cacatttttg cttcctaaat aacttccact gatgatcaac gcttcatccg gttttgctgt
    18781 ctggtacttc gctacaaaga cgatcagcaa catcagtaaa atcaaagctg cgattgcgat
    18841 cgcgataaaa acaactggta tttccattta acacacgctc ctttaagctt ttatcatgct
    18901 ttcataaggc atgacataac agattctttc cttgatctca atgatcaata ctttcgttcc
    18961 ccgctcagct gtctgttctt gggggctgta aaaacaagct ggacgactaa tggaaccggt
    19021 aacacttttc aactgaattt ctcccatacc tcgtacaggg atcggtgtga tcacaacagc
    19081 tacttgtcct tccagagttt tttcagaaga cgacatcgtt gattcggcat gtttcatcgg
    19141 catcaatata tagaaattaa gcaaaaatac caacactatc gatatgcctc cactagcaat
    19201 aaataaaaat aaagatgatt gatctgttaa acgctcacct agaaaaccaa acaagcttgt
    19261 gaaagttaac caagggataa taagcatcgg atcaagcggg ccatcaaaat cgaatacatc
    19321 tccaaaaata atcaatagga ctgccaatcc tgcacaaatc agcaatgtat accaataaat
    19381 cgtctcaagt gcgattcccc ctatcatata cacgcctcct tccatttcta cgtctatcat
    19441 accaatgttt gaaaattctg tatagcttct tttaccgact cagtggtgtt ttttatagga
    19501 aggttgtgtt tatcgttgac aatttgttaa atttttgttt tcatgttaaa attttcacaa
    19561 taaattcttt cgaggtagcc tatgtttcct ttttttaaga aaaaaccatt aaagctccag
    19621 aacacgatca tccttgtcgt ttgtttctcc acattcgttt ctttgattgt ttctgccata
    19681 ttgatcagga attttattct ttcgatgaac cgatctagtg tgtcagataa aatctctagc
    19741 attgctaaaa tcgttgccaa tgataaactc atccaaaaaa agctgcagac tggtgaccca
    19801 acacatgcga ttcaaggaat aacaaatgaa atcatgattg catcgaattt agattttatc
    19861 gtggtcatgg accctgaagg cattcgatat tctcacgcag acccaagtta catcggtctt
    19921 cctttctcaa gtcatgaaga tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa
    19981 aaagaaggaa tgttagggtt cggcttccgc tatttcgttc ctatttatta caatcaagag
    20041 attattggtg cagtttgtgt tggcttaacg aacaacacga ttgacaatca agtcaaagat
    20101 ggacaaaaaa tgattgtttt ttcattgatt atcggactta gcgtaggcat cataggcgcc
    20161 gtttttcttg cccaaaaaat gaaaaaagta ctgcttggac ttgaaccaca agaaatagca
    20221 gaacagttaa gtgaaaaaac gattatcgaa aatgaagtag atgaaggaat cttagccatc
    20281 tcttctgata aaactatcct cttgatcaat acacctgcaa aaaacatttt ctaccaaaca
    20341 gagcagctat tggatttcga agtaggaaaa aaaatcagtg ataatctata tgacgttttg
    20401 tttaaagaga cgattgagca tcaaacgacc gttcggactc aatccatgta tttaaatggg
    20461 atagaaacta tttctagcgt tacacctctt tttagcaatg accgtttttt aggagcagtc
    20521 gcaactttac gaaatcgatc agaagtaatg aaattaatgg aggagcttgg ggggacgaaa
    20581 caatatataa actccatgag agctcaaacc catgaattca tgaataaact tcatgtcatt
    20641 tctggtttga ttgatttgaa gaagtatgaa gaagtcaccc attttatcca gcagctaaat
    20701 caaaattata aaaaagagat tggccaagtc tcaagtctga tcaaagttcc tgcaattgcc
    20761 ggttttttat taggcaaaat aaacgaagcc aaagaacaat ctgttgaaat gctgttagaa
    20821 accgattctt ttatccctaa aatggagatg gatgactcca tccattcact tttgcaagtg
    20881 ttagggaact tacttgataa tgcaaaagaa gccgtgctac aaaaacatga aagaaacgga
    20941 caggtccgga ttcatttaaa ctatgacaca gaagctgaag tattcatcgt gaccgtctgc
    21001 gatgacggtg tcgggatatc agaagaaatc tcagaaaaaa tatttacgct aggtttttcg
    21061 acgaagggaa ttaatcgtgg gtttgggttg aatctcatcc attcaatcgt caaacgccac
    21121 ggtggatttt tagaattttc caaacaaaca aatggcggta caatcgtcta tctagaatta
    21181 ccaaaaaagg aagatcaaaa tgatgacaaa tgtcctgata atcgaagatg atccaatggt
    21241 tgcagaattg aatcgtactt ttgtggaaag catccctaat atgaacgtaa tcgaaagttt
    21301 cagaaatgta gaagaaggaa aaaaatattt cagacaaaaa aacgcagatc tgctgctgct
    21361 tgatgtatac atgcctggcg aaagtggtat ccatttttta tcttggctta gacagcaagg
    21421 aaatgatatc ccagtgattt tgatcactgc tgcagatgat atcgaaacca taaagaatgt
    21481 gatccgctac ggtgccttag attatttgat caaaccgttc actttgcaac gttttcagct
    21541 ggcgatcgaa aagtatcttg ctttctctca gacaatccaa acgactgaaa aagccgatca
    21601 gaaattgatc gatcaagtat tcaaccacac cactcccatt cacgaaatac catcattcat
    21661 cgatttgcct aaaggtctgt taaaactgac tttaaaaaaa ataagtgcat ttattctaaa
    21721 agaagatact attttttcaa caaaagaact ggctcaaaaa agctcactgt cacgtatatc
    21781 agtcaagaaa tatttgaatt ttttggtaga acaagatata ttgaacgaaa gaatcaaata
    21841 tttggaaatc ggccggccgc taacctgtta tgaattgaca aagaatgggc ggaaaaagct
    21901 cacagattac tgttgagtca tcgaattatc ccatacaaaa tagtccaagt atacagaagt
    21961 aacaactgtt tacttggact atttttaaac attttattga cgaatatcca tgttttctta
    22021 tcttaattga cgggctttag gaagagatac ctcatttttc gtttttccag tttctattat
    22081 ttcacgaaag ttctcgaaac ttgtcgaaat catatttgat aatgcctcat ctgtatttga
    22141 acctacatgc ggggttacaa gaactctagg gtacatttct actaattgct ggatagcagg
    22201 gtctggaatc gtctcccatg gcttgaagga tcggaaaaaa acctctgttt catttgcaaa
    22261 aacatcggtt gcaaaaccag ctaaatgatt gcttttcaaa gct
//
LOCUS       DAPBAB010000039        22218 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPBAB010000039 DAPBAB010000000
VERSION     DAPBAB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.039"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            170..514
                     /locus_tag="RD444_001837"
     CDS             170..514
                     /locus_tag="RD444_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDT7549211.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="RD444_001838"
     CDS             515..934
                     /locus_tag="RD444_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDT7549212.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="RD444_001839"
     CDS             complement(1026..1376)
                     /locus_tag="RD444_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549213.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="RD444_001840"
     CDS             complement(1342..2673)
                     /locus_tag="RD444_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDT7549214.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="RD444_001841"
     CDS             2929..3495
                     /locus_tag="RD444_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7549215.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="RD444_001842"
     tRNA            3640..3727
                     /locus_tag="RD444_001842"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="RD444_001843"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="RD444_001843"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDT7549216.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="RD444_001844"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="RD444_001844"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDT7549217.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="RD444_001845"
     CDS             complement(7450..7896)
                     /locus_tag="RD444_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDT7549218.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="RD444_001846"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="RD444_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7549219.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="RD444_001847"
     CDS             8361..8879
                     /locus_tag="RD444_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7549220.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="RD444_001848"
     CDS             complement(8956..9912)
                     /locus_tag="RD444_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7549221.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="RD444_001849"
     CDS             10078..10884
                     /locus_tag="RD444_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549222.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="RD444_001850"
     CDS             10881..11870
                     /locus_tag="RD444_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDT7549223.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="RD444_001851"
     CDS             11867..12871
                     /locus_tag="RD444_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDT7549224.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="RD444_001852"
     CDS             13003..13545
                     /locus_tag="RD444_001852"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDT7549225.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="RD444_001853"
     CDS             13565..14263
                     /locus_tag="RD444_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDT7549226.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="RD444_001854"
     CDS             14284..14496
                     /locus_tag="RD444_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDT7549227.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="RD444_001855"
     CDS             14496..15458
                     /locus_tag="RD444_001855"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDT7549228.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="RD444_001856"
     CDS             15476..15871
                     /locus_tag="RD444_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7549229.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="RD444_001857"
     CDS             16034..16399
                     /locus_tag="RD444_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDT7549230.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="RD444_001858"
     CDS             16396..18207
                     /locus_tag="RD444_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDT7549231.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="RD444_001859"
     CDS             complement(18269..18436)
                     /locus_tag="RD444_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDT7549232.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="RD444_001860"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="RD444_001860"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDT7549233.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="RD444_001861"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="RD444_001861"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDT7549234.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="RD444_001862"
     CDS             21193..22173
                     /locus_tag="RD444_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7549235.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7208 a         3806 c         4620 g         6584 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttt
//
LOCUS       DAPBAB010000040        21958 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPBAB010000040 DAPBAB010000000
VERSION     DAPBAB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.040"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(302..487)
                     /locus_tag="RD444_001863"
     CDS             complement(302..487)
                     /locus_tag="RD444_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549236.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1337..1678)
                     /locus_tag="RD444_001864"
     CDS             complement(1337..1678)
                     /locus_tag="RD444_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7549237.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1678..1908)
                     /locus_tag="RD444_001865"
     CDS             complement(1678..1908)
                     /locus_tag="RD444_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7549238.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2204..2542
                     /locus_tag="RD444_001866"
     CDS             2204..2542
                     /locus_tag="RD444_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549239.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2747..3322)
                     /locus_tag="RD444_001867"
     CDS             complement(2747..3322)
                     /locus_tag="RD444_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDT7549240.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLIINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(3685..4266)
                     /locus_tag="RD444_001868"
     CDS             complement(3685..4266)
                     /locus_tag="RD444_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7549241.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASETGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4459..5085)
                     /locus_tag="RD444_001869"
     CDS             complement(4459..5085)
                     /locus_tag="RD444_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7549242.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYEPVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5107..6438)
                     /locus_tag="RD444_001870"
     CDS             complement(5107..6438)
                     /locus_tag="RD444_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDT7549243.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENTRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6593..6994)
                     /locus_tag="RD444_001871"
     CDS             complement(6593..6994)
                     /locus_tag="RD444_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDT7549244.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7080..7286)
                     /locus_tag="RD444_001872"
     CDS             complement(7080..7286)
                     /locus_tag="RD444_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549245.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7381..8883)
                     /locus_tag="RD444_001873"
                     /pseudo
     CDS             complement(7381..8883)
                     /locus_tag="RD444_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
     gene            complement(8901..9356)
                     /gene="argR"
                     /locus_tag="RD444_001874"
     CDS             complement(8901..9356)
                     /gene="argR"
                     /locus_tag="RD444_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDT7549246.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9454..9861)
                     /locus_tag="RD444_001875"
                     /pseudo
     CDS             complement(9454..9861)
                     /locus_tag="RD444_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
     gene            complement(9876..10070)
                     /locus_tag="RD444_001876"
     CDS             complement(9876..10070)
                     /locus_tag="RD444_001876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549247.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKIG"
     gene            10291..11664
                     /locus_tag="RD444_001877"
     CDS             10291..11664
                     /locus_tag="RD444_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7549248.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEKLQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11739..12851
                     /locus_tag="RD444_001878"
     CDS             11739..12851
                     /locus_tag="RD444_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDT7549249.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTSHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12923..14560)
                     /locus_tag="RD444_001879"
     CDS             complement(12923..14560)
                     /locus_tag="RD444_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDT7549250.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14728..15336)
                     /locus_tag="RD444_001880"
     CDS             complement(14728..15336)
                     /locus_tag="RD444_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7549251.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15720..16871)
                     /gene="queG"
                     /locus_tag="RD444_001881"
     CDS             complement(15720..16871)
                     /gene="queG"
                     /locus_tag="RD444_001881"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDT7549252.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16890..17642)
                     /locus_tag="RD444_001882"
     CDS             complement(16890..17642)
                     /locus_tag="RD444_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549253.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFIPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17782..18732)
                     /locus_tag="RD444_001883"
     CDS             complement(17782..18732)
                     /locus_tag="RD444_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDT7549254.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKGKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            complement(18733..19572)
                     /locus_tag="RD444_001884"
     CDS             complement(18733..19572)
                     /locus_tag="RD444_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7549255.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19569..20315)
                     /locus_tag="RD444_001885"
     CDS             complement(19569..20315)
                     /locus_tag="RD444_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7549256.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20553..21923
                     /locus_tag="RD444_001886"
     CDS             20553..21923
                     /locus_tag="RD444_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7549257.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQTVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAMNGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
BASE COUNT         6115 a         4472 c         3821 g         7550 t
ORIGIN      
        1 tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct
       61 agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccaaaa taacagctga
      121 aaagatagga cttactctta gtaaagtatc tgaactatta atttttaaac tatcattttt
      181 atcctcaact acatacatat ctgttgcaac ttcactttct gtctcatcca gataaatgat
      241 taaggaatcg ggaattgcaa tatcttcagc tgacgctgga atagttgagc caaattagtc
      301 atcatatctt tattataaaa taaccaacag caaaaaatac aatgaaaaac aaacttggaa
      361 ctaatgtatc attaataaat gttgaaaaat tcagcaactc attactgtat gattttagca
      421 gcccaaccaa catataccct ataaaagcta ataagatggt taggaatcct ttagaatttt
      481 tactcattca taaacacttc cttaaatagc ttttattata gaaggtattg taaataataa
      541 tattgacaaa aagtgactca aaataaaaaa aaccttacat tatactggaa tgaactgtat
      601 tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa gtaattttat
      661 taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta cttttacatt
      721 cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg
      781 agcattacaa gtattggtgt cgttagaaca actaaataga aactagcggt agatttttca
      841 gaatctatgc taatttctgt acggatgttc cagtgatggc aaatttctct tttcattgtt
      901 tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct
      961 agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt
     1021 actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc
     1081 tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa
     1141 gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat
     1201 gctatatatt ctttagttta agatacggaa attaatccca tcgaccacac tagtagaatg
     1261 aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag
     1321 agtttactta caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt
     1381 gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt
     1441 gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta
     1501 aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt
     1561 cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct actgagggat
     1621 caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct tctgacacta
     1681 taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc
     1741 ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc
     1801 agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa
     1861 agttaccact acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc
     1921 cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct
     1981 agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca
     2041 gttcattttt tctttaaata aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa
     2101 gctacccaaa tactatagct aactccccgc taccttcaat cttgcagata aaacgctttg
     2161 gaaaaaaatc gagaaaatcg caaagacgac aggccatcct atcatgcctg attataaaca
     2221 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
     2281 aggtgaagaa gtttcaactg ttcggattaa agaatgcccg attcaaatag aaacattggt
     2341 tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt
     2401 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     2461 tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt
     2521 ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca
     2581 gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat
     2641 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     2701 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     2761 ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc
     2821 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     2881 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     2941 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     3001 cttcattcca ctcataaaag ccactcattg ggaaaacaca acgattttgc aaaaatggtt
     3061 gatgaaaggt ctttttttct tctactgtct ctgcacgggc attgattatc aattgtcctt
     3121 ttttaaatcc ggtaaatccc cacttcatga tttccggagt gaggatgttt tcttcctctt
     3181 ttcttaacgt aacaacaaga tttgaaggaa aaacttctcc agctgctaat tctgctgcct
     3241 gctccggttg tttcttctgg gcttctcgat aataattctg aaggagctct gactctagat
     3301 caaaaaaata acgaccacac atcctaatcc ctcccaattt tttttctatc atacactgca
     3361 acaagaagtt cgcaccaaca aaacgctttc aatttgtgta gaactaaaaa agttgtgaca
     3421 agtgacaaac tctccagtga atggtattct cctgccaact cctcggtcac aagtcacaat
     3481 attcaacaaa catgagaagc tgttttttga aattgttcct tgtgacccga agtgaaacgg
     3541 caggcttacg acctctgggt aaacggtgtt caaaagcttg catctgcggt aataagccca
     3601 accaaacgaa aaacatgagc agctattttt atttggctgt tcttattact tgatgcaggg
     3661 cagctttttc acaacctcta attattaaag aaccttttgc gaagttaaca atctttcttt
     3721 gatcaattga aaattttgtc taagtagtga tagatcctgt gtgactttca acaataagat
     3781 acttccctgg atttcagcaa tcaacgcttt ggcttgacgg tctgcttctt catcggacag
     3841 tttttcttca gaaagtgcca ggatattttg tttcgtatac ttgacccatt gattgactaa
     3901 ttctagtagc aacagacggt attcttcatc cactgtactc aactcaataa tcaaattacc
     3961 tacaggacag ccatgataag tgatctgtgt ctcatgatca gcaaaaatcc agtccaacat
     4021 ttcttctaat gcttcttctg gctgttttcc agcatttaag atgtcttcga acaattctgt
     4081 ctgccacacg tccatatgcg catggatcac cgctagacaa acttcttttt ttgaagaaaa
     4141 gtaataataa aattgccctt tccccgtctc agaagctgct agaatatctc ggattgacgt
     4201 ttcatgatag ccttttttat aaataagatc tttggtcgct tccaagatct tttcctttgt
     4261 tttcaatgat attctccttt ttcatttctc atagtcctat attatacaat aaattcatca
     4321 ttttttcttc ttaatcgttt aaaaaagact gtgccaaatt atctgacaca gccctcacta
     4381 taaaactgtt ctcatgtgtt ctcatatagc tagatgttca ttgattcaag aactgcgagg
     4441 acaaaagcag ttcctatctt atgatagttg ctctaatact tcctctgttg tcatcacttg
     4501 atctgcaata aagccaaagt tcgtcaacgc tgcatccatt gcttcttggc caggagcacc
     4561 aattgcatct gtcgctacaa caacattgaa tccactctct accaattcac gcatatggga
     4621 atcgctacac aagtttccgt tcattccacc gagaattact gtgtcgattc cattttttct
     4681 caattgaagc gccaaatcat tgctttctgg tccaaagatt ttatgaggag agctgatgat
     4741 cgtattttca tccaaatatg gtttcaattc ttcgataaaa tcggctcctg aaccttcttc
     4801 gactggttca tattgacttt ttctagcaaa catctgctgc tcaagcatca tatgttctcc
     4861 tgcaccgcca aattgccatt tatggtcatg tggataaaaa taatgaggag aaataaagag
     4921 tttgtatcct ttttcttttg cttgtttcac catttcgatc atattttcga tcgtattgta
     4981 tttttctaag atatcttttg tcaaaccaaa gccaccgcct gctggtttca agaactcgac
     5041 ttgcgggtca gttagaacta ctgctgtatt tttgttgttg aacatgataa attctccatt
     5101 cgttatttat tttaatacat catttagagc ttcactcatt gttggatgag aatagatttg
     5161 atctctcaat aacgtataag gaagttttcc attcatagct agcgaaatca agttgatgac
     5221 ttcatgagaa tcttccgcat agatacttgc ccctagaatc aagtcagttt cagggtcgac
     5281 taaaactttg aatactcctt taggatcttc taatacttgt gctttaggaa caccgcttgt
     5341 catatacttg aataatttgt aattgacgcc ttgtgctttg gcttgttttt ctgttaatcc
     5401 tacattggat aaagttggag tgataaatac actgtaaggc acagctttcc ggttattcgt
     5461 tgttcgtgta ttctcgcctt tcaactgatc catgataatt cgataatcat ctagagaaat
     5521 ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc caaatgttgt ctgcatctgt
     5581 acgaaggtat tcatccacag cgattgctcc gcgatctgtt agtttaacat ctgtattttc
     5641 taatccaagt gatgctgtac ttggttttct tccagttgca gccaaaactt ttgacgcttg
     5701 gattgttact tcttggccat cttctgtaaa ggtgacggca gctgttgatt cttggtctgc
     5761 tacttgatct acagatacac ctagatggaa ttcgattccc gcatttgtca tgtcttctaa
     5821 aatcatttgc gcgatatctt catcttcacg aggaagaaat tcaggatggg catcaagaac
     5881 gacaacttta gatccgtatt cattgaacat tgatgcaaat tccaagccaa tatatccagc
     5941 cccgatgatc actaattttt ccggtaattg ttcctgatcc atcgcttgcg tagaatcaat
     6001 caagtagcga cttgtattca atcctttgat tggtaaaatg attggttcag aaccagtatt
     6061 gatgaaaatc cgttcgcctt caacaatcac tttttcacca ttattcgtca cttcgatttg
     6121 atggttagaa agaaaacgtg ctgtaccgtc catcactgtc ccagttgcct catcgctgat
     6181 catatgataa tttttggctc gaagcatgcc cgtcaatttt tctttccgag caacagcctc
     6241 tgtaaagtcg attcctcttt ctccattgaa aatcaatgat ttagaaggaa tacagccgat
     6301 gttgatacat gttccgccat acatacgtgt tgacttttca acgactaaaa ccgattcgcc
     6361 ttttgaagct aacgcctttg ctaaagtctt accgcccttt ccaaaaccga tcacaatatt
     6421 ttgatatttt tccatcattt ttccctcgct tcttttttac ctaccgattg gtacaaaaat
     6481 atgataaccg ataatcgtct attttcccaa tcatctgctt tatttgtcaa aaaaaaccag
     6541 acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc ttctaaacaa
     6601 tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag aattcatgcg
     6661 catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc acttctaaca
     6721 ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt gcttttactg
     6781 tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca ttgaaacaag
     6841 aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg attcctggag
     6901 gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg cgcccgccat
     6961 ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg attgaccatt
     7021 tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa aaattctgct
     7081 tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa aatactactc
     7141 gtgaacaaca gcgtcatatt tgttccaaaa taaattgcaa tcttttctac ataatctttt
     7201 atatgattga ttttaagata catatatacc agaaagttga ccccataaac caatactgtt
     7261 aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc ctaccagcat
     7321 tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa aaagactggt
     7381 ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg ctaattttac
     7441 taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta gtaacggctc
     7501 atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta gatcataccg
     7561 aataccttct tcatttttcg ggtcgaagtt catccctttt tcttcttcca acagtcgctt
     7621 catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca attttagtcg
     7681 gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta atgtgatacc
     7741 ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg caccatcatc
     7801 cagaaaataa ttttccttga atgcagcgtg tccgcttgtt tcgatggcta gctgtgtatc
     7861 tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc cggaaatata
     7921 acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag aagtagggga
     7981 attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta atactgcgat
     8041 caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac gatccacatc
     8101 tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct tgatactttc
     8161 catcgcttct ttattatcag gatttgggat atgattagga aatcggccat ctggttccaa
     8221 aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag caaagaatcc
     8281 accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt tgctttctag
     8341 tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg tcaaaaggtc
     8401 tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg ataggataaa
     8461 agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc cattataata
     8521 atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact gttcaaattg
     8581 agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat gtatcccttg
     8641 ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc tactgtcatg
     8701 accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt tctttatcca
     8761 gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta cttcatatcc
     8821 ttcaactggt aatgcgattc ctcgaatatc cgatccattt tacagcgctt tcatatcttt
     8881 catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc aaatcgctgt
     8941 gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat cgttccgaca
     9001 acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc atttcgttca
     9061 gtcaatacga tgaccatgaa ctgaacagac tggatacgca aaacctcgcg tctaattgct
     9121 gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt atagcgtact
     9181 attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg agaaatcgtt
     9241 gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc ctgtgtccca
     9301 ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt tttcatatta
     9361 gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata ttttggtatt
     9421 ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt taacacgcaa
     9481 aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa tctctttgtg
     9541 ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt tctcaaacgt
     9601 tgctggttct tcgtcatagg gcccgacgtg taagatttgg gcacaaagac cttcttccat
     9661 ctttttgaat actacttgtt ctttcaagtc ttccggaagt ttgctagaac gctgaatggc
     9721 ttcttgtgct atcgcttctg taacaaagtc aggctgtcag atcatcaatt gataagaaaa
     9781 atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc attgtccttc
     9841 cagtggatag acagtataca tctgataatc aggaatcagc caattttttt aggcgacatg
     9901 cgtattgcat agctgactgc atatagacag gctactcttc gtttaaagtc ttcctgattc
     9961 gggtcgccga tccctctgat gctaaagaat ggttttttag gaacctctag tatttgcggg
    10021 ctttttttca tactatagct cttgtcttta cgccattcaa ttttttccat ttttcttcct
    10081 ccgcgaaatt ccatttagtt atttattatc atacctcttt ttgagtgact tggctaatcg
    10141 tgttgacact atttttcagt ttgtgctaga ataagtgaag taaataaatc gtacagataa
    10201 cacgtttcgg cgtgtcgaga gagaggcggt ttctattttt ataatagaaa tcactctctt
    10261 tttttacgaa attttttaga ggtgatgtaa atgacaaaac aaaaacagct gcttttagta
    10321 cctgcagtaa ttgcaacagg gatgatgtct tttgcgggcg tattgatcga gacggccatg
    10381 aatgtaactt tcccaactct tatcagacag tttgatttaa cgactgcaca agtccaatgg
    10441 gtaacaacga tttatctttt gatgatttcg atcattgttc cgctgtctac ttatttgaat
    10501 cgcaatttta gtctcaaaaa actcttttta gcttcaaatc tgcttttctt ggcaggtata
    10561 atgatcgatt tcttctctcc taccttcatg gtcctattgt ttggtcgatt gctccaaggt
    10621 gcagcgacag gtatcgctct tccgttgatg ttccatatta tcttgacatt tgcccccctc
    10681 aatcgacgag gcgcgatgat gggtgtcggt actttgacaa cggccattgc tccagcaatt
    10741 ggtcccactt atggaggcat tatgacttca cattttacat ggaatcatat cttcctgttt
    10801 cttgtgcccg ttttgattct ttctttgatt atcggctggt ttgctattcc cgagatggca
    10861 gtacaaaaaa gtggttattt ggattggata agcgttttag gaatttgtct tttgtttagt
    10921 ggaagtctaa catttttgag tacgttggga acagtttcca gctggattgc tttaatcatc
    10981 ggcggtattg gctggttcct tttttactat cgaagtaaaa aagtggagca tccgctgatc
    11041 cgactgtcta ttctttcaaa taaaacattc cgcttgtttt tgattggctt cttagtttat
    11101 cagtttttat tacttggtgt atcttttgtc ttgccgaatt tccttcagat cgttcaagga
    11161 agtagtcctt ttgtcgctgg attagcgatg cttcccggcg cagcggttgg tgctttgctt
    11221 tctccttttt ccgggaaaat gctggatcaa tatggtccga agaagccaat ttttgctggg
    11281 ctgattcttt ctttgattgg ttggctagca ttattcctac tcataggaca cgcttcagtg
    11341 ggtgtattga ttgcttgtca tgtgttttat atgatcggta tcggattatc ttacagcaat
    11401 atgatgacca ccggaatgaa tgcactgaat gaaaaactac aaggagacgg gaacgcaatg
    11461 ttcaatacct tgcagcagtt ttctggcgct gtggctactt cattagtagc ggtcattatc
    11521 aattacgtcc agcatcatac ttcccataac tatgaagtgt ctactacatt aggatcaaaa
    11581 gcagcattag gcgtcttgtt gctgttgctt ctcgtaagct ttgctcgatt cgcctattat
    11641 ctgttctttg ctaaaaaagc gtagattcca tatacggtag gtgttgcatc tgccgttttt
    11701 ttgtgtgaaa atggttgttt gtaaaaggag gcttttttat ggaaaaaatt gccattatcg
    11761 gtggcgggat cattggtatg accctagcca gttacttaga tacatcaaaa ttcaatgttg
    11821 ctttatttga tgaagggaca ggacaggcaa ctaaagctag cgctgggatt atctcccctt
    11881 ggttgtcgaa acgcagaaac aaacaatggt atcgtctggc taaagacggc gctgcttttt
    11941 tccctaaact tataaaagat ttccaacttg atcaaacgat ttacgcacaa tcagggacac
    12001 ttttgcttcg acctgaaaaa gatttagctg aattggctgt tcttgccgag aaacgaaaag
    12061 aagaagcgcc agaaattgga aacatcgaat ggctttcacc tgaacaaaca tctcaagcac
    12121 ttccgttatt gaaaccagca gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa
    12181 aagcatattt agaacatctt ggtcagcgtt tagcacaaaa acaaatggag cagatccagg
    12241 caaaagtcac ttcgcataag aagagcgatt cttggctcat caagggaccg tttggagaat
    12301 acacagctga tcgtgtaatt ttgacacctg gcccggcact taagccttta ttacaagaat
    12361 taggctatgc tgcagatata cgtccgcaaa aagggcagct cttagcattt cagacatctt
    12421 ataagatgag tcaagactgg cctgttgcta tgcttgacgg agaagctgat ttcattcctt
    12481 tcatggatgg aaagatcctt ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg
    12541 ctcctactag cgaagcttat aagggattga ctaagagtac agaacagttc ttgactgatc
    12601 cagatcagat aaaaaactgg ccatacgagt tccgtgtagg tacccgtgct tatacgtcag
    12661 atttttcccc atttttcggc gtactgccag atgatccaag cttactggct gctagcggtc
    12721 taggctcttc tggcttgacc acaggtcctt atatcggcta tctattagcc aacttcttaa
    12781 atactggtca ttggcctacg gaagattatc aaaaggacat ctccacctat attacgaaaa
    12841 atccatcata gaaaaacagc tttttcaagt aaggatcccg agcgatattg cgcatggatt
    12901 acttcttgaa aaagctgtta ttttagttca atttctgaat caaattctcc aaactttctt
    12961 ttgcttcatc tggcaagaga tcttttccta tctgagcttc aatgaatcgt aaacgaatat
    13021 caagtcgttg tttcaccaga tttttatagg acgtatcctc aaaagaaaca ggaaaatctg
    13081 gtataggtaa atagtggatg tcttcaaatg gaccaaacga cttgaattct gccgtttgtt
    13141 gcacgattct ttcaacgatt gacaatgtcg tttcaggttt gattttttta tcatcgaaat
    13201 atccagtatt caggacatag caggctgttt gctgtttcaa aaacaactct cgaaaagctt
    13261 gatagtcttc cctaagagga tagacacgga aaggattagc aaatggttca atgactagct
    13321 catctagctg tctttttgta gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg
    13381 caccaaaaga cgctgctaaa accgggtcat tgatcttgat aattggcgga agcgaatcat
    13441 ctttcatgat ccaaaaaaca gcatcgatct tttcgctaag atgatcgact cggtcgggtg
    13501 tcgcataacg tgatttgact gtccgcccat ttccgtttcg tatatctcct gttactagga
    13561 ctttttctcc tttacttgta catgtcacgc ccacattctg acaagtaagg aaagaagaaa
    13621 tggccggatc atcagacgga tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag
    13681 tagcaaaacc attttttcga gaaataacga aagcatcgtc atgcaagatc gaaacagagt
    13741 gaccttccgt atgttttgct aaggtaatcg ttgattttcc agaaccggac aacccaaata
    13801 cagccatcgt ataggtctgg tcattactag catattgttt catcccaccg tggcaggcaa
    13861 taaaaccatt gcgatttccc aatgcccaag caagcgtcaa agtcgctttt tttaattctc
    13921 caaagtaacg taatcctagc acagccgctg tattatggag agcatcaaac aaaatgagtc
    13981 cattgggaaa tgtttcatgt gtccactcgg gatcacaaaa gatataaaga tctccctcat
    14041 caaaggaaac agacttttga taagtcttta agatcttcga attcgctggc tgaaaattca
    14101 gcaaataaga atacaaattg ttctcaaatc cttctggaat cagcaaatga ctttgcacag
    14161 aaaattcttc agataggcca acgattactt ttgtatggta aaactggcgt ttgcgacttt
    14221 gatacacagc ttctcttaaa attttctcgt atttgctttg ttccttgtct aagtcctcaa
    14281 agatccgccg agcacttgct gttcttccca caatgcttcc atcattgaat actagtgtcc
    14341 tgacatcagc tggtaaatct agttcttcag catgtgagat tgttaaatct gtgatgatcg
    14401 ttcctggact cccttttgct agttgataag ctgtcttcct gtcaaaaact tcatgaacat
    14461 tattaccgta aaatgtcgtt tctacaatcg atcgaatact ggataacagt ggatttccac
    14521 ttgtgatttc gtttaattcg agactctctt ttgtactcat ggctcctcct cattccctaa
    14581 acaaaaaaac gttttcttta ttatacatta ttcgagtttt tatttgtact gctgcgctgt
    14641 gggaagcaaa cgagttattt ttcacacaaa aaaaccgaga acttgaataa acaagtttct
    14701 cggtttcatc attttccttt tatcggctta gtaccagccg tttgctaacc agaatgtttt
    14761 agcagcatcc catgaaccat aacgagatgt tacatattgt tctgctacac gttcttgatt
    14821 ggcagctgaa tagtcgccgt ttaagtatga agcatctaat tgataacggc cgatgtaacg
    14881 tccgttagta gctgtgtatg aaccgcctga ttctttttgt gcgatccatt cttttgcaga
    14941 gcttgaagtt ggcgctgttg cagcagctgg ggcagcagga gctacttcaa ctgtttcaac
    15001 aggtgcaacg tatgtatgtt cttgcgttgc tgtttcagtt gtagatgctg tattttctgt
    15061 tgttttgttt tctgttgttg ctgaagaatc atttgaagtt gggattgtta attgttcacc
    15121 aacataaatg cggttgatgt ttgcgatcga atttttttca gcaattgcat cgatcattga
    15181 gttgtctcct gcaaattttt gtgagatctt agataatgaa tcacctgatt ttactgtata
    15241 aacttcatca gcgtgagcat ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa
    15301 agttgtacct aaaacaattt tcttaaatga attcatatta aactcctttt tttctccgtt
    15361 ttttattctt tcgacgctat taactttatc atttagaaat atttcatagg taaaagaatc
    15421 atgacattta atgacaaaat gacgaattat ttaatatttg taacatttaa acaaatattt
    15481 ttttgataca aaaccataaa aaccttgttg tataagcaaa gtaatcgttt ttctatgttt
    15541 ttttattttt gaatgaaaaa cgcagattct gtaacattta ctgcaataat ccataaaaaa
    15601 aagcaaaaaa atttttgatt tttatcaaat tctcttaaaa aaatgtaaaa aatacaaaaa
    15661 aaccagtaat tgttacaaca attactggac aaaaactttg aataatgtta actatttttt
    15721 tattttaact ttctccgcaa taatttctgg gcatgttcca attcgtttat aacgacttct
    15781 tctgtttctt tagctaatat ttcatctaat acatcaagcc atttttccgg ttcttttgtt
    15841 cctagacgtc caatagccca agctgctgtt cctctgatga cagggcggac atctgttaaa
    15901 cattctgaaa tctcaggcaa tgccgttcga tcacctagat tagctaaagc aatcaatgcg
    15961 tttctctgca aaggtttctt cccacgccat gaaccagcta aatgcccaaa ttgctgtttg
    16021 aagtctttat tagacatcga aagcattggc tttaattttg gatatacttc gtctactttt
    16081 ggttccatct cttcatgaaa atgaaaatct tttccttgat tgtaagggca gactaactgg
    16141 cagatatcac atccataaat gacatttcgc atttttttac gatattcttc cggcatcatc
    16201 ccttttgtct gagtttgata agacaaacat cgctgggcat tcattcttcc gtcaccaagc
    16261 aatgcttttg tcggacaagc agtcacgcag cgcatacagt caccgcaacc aaattctcct
    16321 ggtttatcag gctcaaatac aatattagta acgatctcac caagatagac aaatgaaccg
    16381 aactcttcag tgatcaacaa tccgtttcgt ccaataaatc ctaaaccagc tcgctgtgct
    16441 acagcgacat cgatcagttc tccagtatct acttgcggag ctagacgcca ttcctcttct
    16501 ttttgccatt tttctgctct tgtttggatg aattcaatca atcggttcaa acggtctctg
    16561 agtatatcat ggtaatcgat cccccaagaa gcacgagcaa actgtccgcg tttttcatcc
    16621 ctaggcactt cttcatggat cctagtagga tacgcaagtg caatggaaat gatcgatttc
    16681 ggctgatcaa atgtcatttc aggataaaga cgctcatcga tatttggatg ttcaaagcca
    16741 gaagtatggc cagctgcttt ttgttcttcc aacgattctt ttaatgaatc aaaaggttcc
    16801 gccgtggtga acccgatttt gtcaattcct aatcgtttgc tttcttgtat gatttcttct
    16861 tttaatgtca tggtcttcct cgcttgctgt taacggctgt ataacccata atatagtttt
    16921 tcttttttca gctggacaaa ctggatctgc tgatcggcaa tcttcttttc ttccatccat
    16981 ttttttcttg cagcaaatac ttcccaataa tttgttgaaa ttaattttct tttgttatct
    17041 aatgcattaa aaaaattcaa tgaaactggt tttgtactta acagttcatg gagccttacc
    17101 tcttcccatg gcgaaaactc agacagatcg cgggaataca atacttcttc atagagattt
    17161 ttttgctgtg tgtacgtgaa tgtttgcggg attttcccag gcctggcaat ttctactaga
    17221 cgattataaa acatattcat taatttgctg ttggcaataa attgactttt ataataggcc
    17281 gactggatct ttttgattac tccttctgct ttgatgactg aaaataatgc aggccatgct
    17341 ttgacggtta tttcgtcgtt ttgtttaact aatacgccag gtatgaagcc gactggagaa
    17401 aggaacacaa aataaccgat accggtattt tcatctaaag gctgaaattc atcaaacaac
    17461 catttgccta tgtacagctc ttcatctggt tctgttgtct cattttgatt tgctatgaat
    17521 aatttagcta attctgcttc aagaaattga aggtcttgat ccttgtcggt attttcctgc
    17581 tggctgatca atcgaaagac ttcttcttta atcgtatatt tatctttacg tgtaaaattc
    17641 atagccacac cttccttcct tactctaact ataacggaat ttcttcaaaa aaggtatctg
    17701 tacgccgaaa aacagcataa tttttttcat atctcttata aaaaatgcaa ggcctgctcc
    17761 tcaagctaga ccttgcactt ttcagctttc ttttatttgt gacaaacctt catggatcgt
    17821 ttcaatattc catttcatca tctgatagta agaatctcct tgttctcctt cttttgctat
    17881 ggaatcagtg aacagtttat catagatttt caaacccgtt tcttttgcta cccgttccat
    17941 gcttcttgag tctacactgg tttccacgaa taaaaccggt acttcttttg ctcttatctg
    18001 atcaatgatc gctttcattt gatcaggcgt accttgactt tctgtattga tctcccatat
    18061 ataagctgct ggcaaatcat atgctcgtga aaaatacttg aatgcccctt cacttgttac
    18121 taataatttt ttgttctctt cgatagaagc aaaactttcc ttcgcttgtg tatctaattc
    18181 ttttagtttt tccacatatg cttctgcatt ttttttatat agtgtcgcat tttctgcatc
    18241 tttttcagag aatatacgtg cgatttcctc cacatatttt attccgttag ataggtctag
    18301 ccaagcatgg ggatctgctt ttgtatgttc ttcaccgcta gttaaataaa gaggttctac
    18361 atttttgcta acagcaaaat aatcttttcc ttcttctttt ttagccgttt ccatcaagtt
    18421 atcgaaccag ctgttacctg tttcaagatt caaaccgttg tataaaataa catccgcatc
    18481 gcttgccttt ttgatgtctt ctggtagtac ttcatattca tgcggatctg ttccgactgg
    18541 tacgatactg tggatatcta tttgatctgt tccgacctct tttgccatgt ccgccaagat
    18601 cgaattggta gccacgactg acaactttcc tttacttcct tcatccgctt ggtcagaagc
    18661 ctgctttccg caaccggcta ataccaagaa actaactgcc aagattaaaa agagtgattt
    18721 ttttgttttc atctatctgc ttcctttctg aaataaaatt ccttttgttg gagaaaataa
    18781 aaaagcaagg ataaaaaatg cggtacatgc taacacgatc gtagctcctg atggccaatt
    18841 gtaactataa ctgaaaaaca caccgacaac tgcacttagc ataccgattc ctgcagctgt
    18901 aatgatcatt ttcagtaaat ggttcgttag caagtaagcc gtggcggctg gtgtgatcaa
    18961 catggcaatc accaagattg ttcctactgt ctgtaaactg actacagcca ctaatgtcaa
    19021 aaagaacatc aaaagataat ggatcagcga tgtgttcaac ccataagctt ttgccattgt
    19081 cggatcgaat gatgtgatct tcaattcttt ataaaagaaa aagacaaata tcaagacgat
    19141 tccactaacg agtgctgtca gcaacaaatc gctttcccta acagctaaga cattcccaaa
    19201 taaaatatga tacaaatctg tcgcactctg ggcatatgaa atcaagataa tccctaatgc
    19261 aaaaaaggag ctaaagacaa ttccaatcgc tgtatcattt ttcaatcggc tccgctgagt
    19321 cacgaaacca atcactgctg ctgttagcat cccaaaagca gtggctccaa agatgtaact
    19381 aaaaccaaac atataagaaa tggctacacc tggaagaact gcgtgagaga tagcgtctcc
    19441 catcaaagac atccctcgta aaataataaa agaaccgatg actccagaag caagtcctac
    19501 aagcatggaa gtgaccaaag cgtattgcag gaattgataa ttcattaatc catcaataaa
    19561 ggattggatc attttttctc tcctcctttt actaaatgtc tgatgatctc accataggcc
    19621 aattgaaggt tcttcgatgt aaagacttct tgtactggac cggaagcgat cagctgttta
    19681 tttaaaagaa taacctcatc gaaatattct tctactttat gaagatcatg atggacgatc
    19741 aaaatcgttt ttcctgaatt cttcaattcc tgcaagatgt caaagatctt tcgttcactt
    19801 aacgcatcaa tccctacgaa tggttcatct aaaaagatcc attctgctcc ttgggctaga
    19861 gctcttgcaa taaaaactct ctgaagctgt ccacccgata gttcgctgat ctgtcttttg
    19921 gcatattctt ccatccctac ttttttcaaa gcttgtcttg cacgttcttt ttcttttttc
    19981 ccaggtcttt gtccaattcg taaagatgga tatgttccta aaagtacaac gcctaataca
    20041 tcaattggaa aggaaagatc caattcactt cgctgttcca cgtaggctat ttttttacgg
    20101 actgagttta ccggctgtcc agaaaaggtg actttaccag tagtagctgg tatcaactct
    20161 agcaaagatt tcataaatgt tgattttcct gcaccgtttg gtccgataat accggtaatt
    20221 ttttgtggat ttatcgtcaa gttgatagtt ttcaaaacag ttttcccttg ataagcggct
    20281 gaaaggtctt gaattacaat ggccgtacgt tccaaatctt acactccttt ttaggttaac
    20341 ctaacttttt atttaaatat agtttaacta aagtggaaag cattttcaag tagaatgatg
    20401 aaaaagtttt tttcgaattt tcctcgcatt ttatgaaaat tttctgtaga atcacatcaa
    20461 gagttcaata aggctaacct aataatgaaa aacatgtgtt gaaacgtttg attatttttt
    20521 cttgttgact caacaacgaa aggagttttt gtatggaaga agaaaagtta actcgaagtc
    20581 ttagtgcccg tcatatccaa atgatcgcac taggtggaac gatcggtgtc ggtctgttta
    20641 tgggtgcctc ctcaaccata cgctggacag ggccctcagt gatgggagcc tatgcgattg
    20701 cagggttctt tttgtatttg attatgcgtg ctttaggcga aatgctttac gttgatcctt
    20761 cgactggttc ttttgcaaat tacgcgagcg agtacatcca tcctgtggct ggctacttga
    20821 ccgcttggag taatattttt caatatattg tcgtaggaat aagtgaagtg attgctgtag
    20881 gatcttatat gaattattgg tggcctgatt tgccggcaat tattcccggg atcattgtcg
    20941 tcttgtttct tatgctggca aatcttattt ctgtcaaggc gtttggcgaa ttagaatttt
    21001 ggttttcgat catcaaagtc ataacgatca ttttgatgat cattgcaggt cttggggtaa
    21061 tcttatttgg atttggtaat catggtcaga cagtagggat ttcaaatctt tggaaaaatg
    21121 gtggattctt cactggaggc gtcaaaggat ttttctttgc attatcgatc gttgttgctt
    21181 cctatcaagg aatcgaactg attggaatga ctgctggtga agcagagaat cctaaaaaga
    21241 cgatcattga agctgtccag tccactatcg gtcgaatctt gattttttat attggtgcta
    21301 tttttatcat tgtgagtatc tatccgtgga atcaattgag tgagattggt tcaccttttg
    21361 tacaaacttt ttctaaagtc ggcattacat ttgctgcagg actgattaat tttgtcgtga
    21421 ttacagcggc attatcaggt tgcaattcag ggattttcag cgcaagtcgt atggtctata
    21481 cacttgccat gaatggaaag atgtctaaaa aatttctgaa actttctcgt cacggcgcgc
    21541 ctttttatcc tgtcgtagca atctcaggtg gaatcttaat tggtctgatc ttaaattatc
    21601 ttctaccaat gttcatcaac cagtcagaaa atattttcgt tttcgtatac agctcaagta
    21661 ttttgcctgg tatggttcct tgggtagtga ttttgatcag cgagattcgc ttcaaaaaga
    21721 agcacgcaga taaaatgcat gatcatcctt ttaaaatgcc actatctcct tatacgaatt
    21781 atgtgacact tgctttttta gctatcgtat tgattttcat gtttatcaat cctgaaacga
    21841 gaatctcatt gattattgga ctcgttttct tgctttacat gacgatccat tactttgtac
    21901 gcgaaaaaag aaccggaaaa taaaaaaaga acgaagcttc accttagtgg aagcttcg
//
LOCUS       DAPBAB010000041        21617 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPBAB010000041 DAPBAB010000000
VERSION     DAPBAB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.041"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            68..808
                     /locus_tag="RD444_001887"
     CDS             68..808
                     /locus_tag="RD444_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7549258.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="RD444_001888"
     CDS             complement(1232..1402)
                     /locus_tag="RD444_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7549259.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="RD444_001889"
     CDS             complement(1596..1775)
                     /locus_tag="RD444_001889"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549260.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1785..1961)
                     /locus_tag="RD444_001890"
     CDS             complement(1785..1961)
                     /locus_tag="RD444_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549261.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2162..3274
                     /locus_tag="RD444_001891"
     CDS             2162..3274
                     /locus_tag="RD444_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7549262.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="RD444_001892"
     CDS             complement(3352..3645)
                     /locus_tag="RD444_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDT7549263.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="RD444_001893"
     CDS             3813..4127
                     /locus_tag="RD444_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDT7549264.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="RD444_001894"
     CDS             4148..6127
                     /locus_tag="RD444_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7549265.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="RD444_001895"
     CDS             6140..6466
                     /locus_tag="RD444_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDT7549266.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="RD444_001896"
     CDS             6482..7000
                     /locus_tag="RD444_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549267.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="RD444_001897"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="RD444_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDT7549268.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="RD444_001898"
     CDS             complement(8436..9941)
                     /locus_tag="RD444_001898"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDT7549269.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="RD444_001899"
     CDS             complement(10033..10623)
                     /locus_tag="RD444_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7549270.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="RD444_001900"
     CDS             10802..12448
                     /locus_tag="RD444_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7549271.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="RD444_001901"
     CDS             12497..13216
                     /locus_tag="RD444_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDT7549272.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="RD444_001902"
     CDS             13695..14642
                     /locus_tag="RD444_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7549273.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="RD444_001903"
     CDS             14654..15574
                     /locus_tag="RD444_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7549274.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="RD444_001904"
     CDS             15615..17072
                     /locus_tag="RD444_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7549275.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="RD444_001905"
     CDS             17417..18031
                     /locus_tag="RD444_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7549276.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="RD444_001906"
     CDS             18024..19748
                     /locus_tag="RD444_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7549277.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="RD444_001907"
     CDS             19745..21196
                     /locus_tag="RD444_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7549278.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6973 a         3674 c         4365 g         6605 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac agttcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tattttt
//
LOCUS       DAPBAB010000042        21592 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPBAB010000042 DAPBAB010000000
VERSION     DAPBAB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.042"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            175..402
                     /locus_tag="RD444_001908"
     CDS             175..402
                     /locus_tag="RD444_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDT7549279.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            418..1584
                     /locus_tag="RD444_001909"
     CDS             418..1584
                     /locus_tag="RD444_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDT7549280.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1819..2862
                     /gene="hrcA"
                     /locus_tag="RD444_001910"
     CDS             1819..2862
                     /gene="hrcA"
                     /locus_tag="RD444_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDT7549281.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2956..3519
                     /gene="grpE"
                     /locus_tag="RD444_001911"
     CDS             2956..3519
                     /gene="grpE"
                     /locus_tag="RD444_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDT7549282.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3570..5399
                     /gene="dnaK"
                     /locus_tag="RD444_001912"
     CDS             3570..5399
                     /gene="dnaK"
                     /locus_tag="RD444_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDT7549283.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5550..6716
                     /gene="dnaJ"
                     /locus_tag="RD444_001913"
     CDS             5550..6716
                     /gene="dnaJ"
                     /locus_tag="RD444_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDT7549284.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7084..7821)
                     /locus_tag="RD444_001914"
     CDS             complement(7084..7821)
                     /locus_tag="RD444_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7549285.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7823..9325)
                     /locus_tag="RD444_001915"
     CDS             complement(7823..9325)
                     /locus_tag="RD444_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDT7549286.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9584..11578
                     /gene="uvrB"
                     /locus_tag="RD444_001916"
     CDS             9584..11578
                     /gene="uvrB"
                     /locus_tag="RD444_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDT7549287.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11588..14404
                     /gene="uvrA"
                     /locus_tag="RD444_001917"
     CDS             11588..14404
                     /gene="uvrA"
                     /locus_tag="RD444_001917"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDT7549288.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14667..15932
                     /locus_tag="RD444_001918"
     CDS             14667..15932
                     /locus_tag="RD444_001918"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDT7549289.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15936..16667
                     /locus_tag="RD444_001919"
     CDS             15936..16667
                     /locus_tag="RD444_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDT7549290.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            16802..17686
                     /gene="rapZ"
                     /locus_tag="RD444_001920"
     CDS             16802..17686
                     /gene="rapZ"
                     /locus_tag="RD444_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDT7549291.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17683..18681
                     /locus_tag="RD444_001921"
     CDS             17683..18681
                     /locus_tag="RD444_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDT7549292.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18715..19650
                     /gene="whiA"
                     /locus_tag="RD444_001922"
     CDS             18715..19650
                     /gene="whiA"
                     /locus_tag="RD444_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDT7549293.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20193..20843
                     /locus_tag="RD444_001923"
     CDS             20193..20843
                     /locus_tag="RD444_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549294.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            21030..>21592
                     /locus_tag="RD444_001924"
     CDS             21030..>21592
                     /locus_tag="RD444_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDT7549295.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         7103 a         3658 c         4712 g         6119 t
ORIGIN      
        1 ttttatgaat aaaataagga caaaattgtt taggaagaat catgagtgta aaaaaatcat
       61 ccaagaagtc gattcttgga ttttttagtg gatttgttcg ccaaatacgt ttagtatgcg
      121 tatatgagag gttgtaagca aagcgttgtg gtctgagcac ttaaggagga atctgtgaaa
      181 atagcttttc atatttttgc agattttgac ttaatgccga aggtcagctt tgcgattacc
      241 gtttatcgag aggctgtaaa agcaccgtgt agctccgatg acaataagca agcattttta
      301 gagctaggtt tccttgattc taaatatgcg gcttatttca atgagttggt gcttgaacgc
      361 gattttaaga aggtcgaaaa caggcacgac ttttcgacgt aaggagaaag aatttcaatg
      421 atagaaacaa taaatcccgc agaaaacaag gtttctgcgt acattcatat cccattttgc
      481 gagcatatct gctattactg tgactttaat aaagtttttt tagaagggca gccagtagat
      541 gagtatgtgg aaatgctgct gaatgaaatg agattaatgc tggaaagtca tcctacagcg
      601 aatttggaaa cactttatgt aggaggcgga acaccaactt ctttatctgc taaacaactt
      661 gatcgactat tatcaggtgc tagagagatc ctgccttttc aaaatggtaa tgagtttact
      721 gtagaagcga atcctggtga tttgacccgt gaaaaacttc aagtgatgaa aaactatggc
      781 gtcaatcgtt tatccatggg agtgcagaca tttgacaatc gcttattgaa aaaaattgga
      841 cgtaaacata ctgcagaaga tgtgtatcaa acaatgcaat ttttagaagc agaaaatttt
      901 accaatgtaa gcatcgattt gatttatgca ttaccgggac aaacactaga aggatatcgg
      961 gatactttgg aaaaagcttt ggctttggat ctgcctcact attctttgta ctcattgatc
     1021 ctcgaaaaca aaacaatgtt catgaactgg gtacgacaag gaagactcga actgcctgat
     1081 caggaaacgg aaaccagaat gtttgaagag acgattgaag caatggaaaa gcatggacgt
     1141 caccaatatg aaataagtaa ctttgggctc tccggacatg aatctcaaca taacctcatg
     1201 tactggaaca atgaccacta ttttgggttt ggagctggtg ctagcggtta tcttggagat
     1261 attcgttacc gcaacaaagg accgatccaa cactatttac gcccattacg agcaaatgaa
     1321 cgcccggtat tggaagaaga aaaattgaca cgaaaaaatc aaatagaaga agaaatgttc
     1381 ttaggactaa gaaaaaaaac agggattact aaaaaacatt tctatgatcg ctatcaaaaa
     1441 acaattgaat cggtgtacgg tgatgtgcta agggaattag agcaagaagg gttacttgtt
     1501 aatgaagaag accgtgttta tttgacacca agagggactt ttttggggaa caatgtgttt
     1561 gagcgctttt tatttgaaaa atagtttgaa gaatatttgt tttttatcga aacttgaaaa
     1621 gaaaaagttt agaaagtttg acaatgaggt attgaagtat tgatataact ggatttacat
     1681 caattattta gaattagcac tcttgtgttt agagtgctaa aaaatggagt ctttctcttg
     1741 acaggagaag ggcttcttgc tatatttata agtgaattag cacttagaat atatgagtgc
     1801 taatgagagg tgaggaagat gttaacaaag cgacaaaacg acattttacg tctgatcatc
     1861 caacactata cgagtagtgg cgtgcccgtt ggttccaaaa cattaatgtc tgagggagtc
     1921 aaggccagct cagcaacgat tcgcaatgat atgaaagcat tagaagatga aggattgctg
     1981 ttgaagactc actcttcttc tgggcggatt ccatctgctt taggctatcg ttattacgtg
     2041 gatcatttgt taaaacctgc tcgcgtagca gaagatgatt tgctgctgat tcgtcagtct
     2101 ctaggcagag aatttcatga aatcaatgag attattaagc aatctgctga gatattatct
     2161 gagctgacaa gctatacgac tttttcattg ggacctgaaa taaaagaccg ccgactgact
     2221 ggctttcgta tcgtaccctt gaataatcga cagattattg ctatcgtcgt aacagatatg
     2281 gggaatgtag aaagccaagt gttcactctt cccgaatata tgggaagtca ggaactagaa
     2341 aaaatggtac agattgtaaa cgaccgtcta gtcggagatc ctctgatgac ggtgtaccat
     2401 aaacttcgta cggagattcc gatgattctt cataaatatt tccagacgac agaagggatg
     2461 tctcagttat ttgacaccgt tcttggtcat gtttttgaag aaaaggttta tgtcagtgga
     2521 caaatgaatc tcttggatta tgagccccgt caagatcttg atcagttcaa atcgatgttt
     2581 tcttttatga aagactcaga cggacttaca caaatgatca ctccgatgga tagcaatatt
     2641 catatcaaga ttggtgctga attaggcaat gatttattgc agaatatgag tatgattcaa
     2701 gcaagttacg agatagatgg tcatggaaga ggtacaatag ccttgttagg gcctactagt
     2761 atgccttatt ctcggatgtt cggtctagtc gatgtttatc gaagagaact agcagctaaa
     2821 cttgctgatt attaccgctc gcttgacttg tcaaactcgt aatgtgaata acgaagctat
     2881 ttgcagctga tcgttctctc tttcttagta agagcaatca aacagctgac tgaataatga
     2941 aaggaagact gtatcgtgaa agaaaatcaa gaagaactag ataaagaaat gacagatgct
     3001 cagccagaac cggaaatcga tgtcgaagct gcagaagatt cgggtatttc agaagctgaa
     3061 gcggaagaat tcgaaacagc gaaattaaaa gctgagttag aagaaatgga agatagatac
     3121 ttgcgggctc gtgctgaaat cgcaaatatg gcaaatcgag gaaaaaatga gcgtgagcaa
     3181 ttacaaaaat accgttcaca agatttagcc aaaaaattac ttccatcaat tgacaacctg
     3241 gaacgtgcat tagccacaga agtgtcagat gatcaagggg caggtttgaa aaaaggcgtg
     3301 gaaatggtct tggaaagttt gcgtaacgca ctggaagaag aaggaattga aaaaatccct
     3361 gctaaaggag aagcctttga tcctaatctt catcaagcag tacaaactgt tccagcgact
     3421 gaagatacac cagccgatac gattgtagaa gtactacaag aaggctacaa attacatgat
     3481 cgcgtgttaa gaccaactat ggtcattgtt gcacaataat tgtgcggata taaaacttac
     3541 actttcaatt aaagaaggag acgttaatta tgagtaaaat tatcggtatt gacttaggta
     3601 cgactaactc tgcagtagca gtattggaag gaaacgaagc aaaaatcatc gctaacccag
     3661 aaggaaaccg tacaacacct tctgttgtat cattcaaaaa tggagaaatc caagtagggg
     3721 aagttgcaaa acgtcaagct gtgacaaacc caaatacgat ttcatcaatc aaacgccata
     3781 tcggtgaagc aggttacaaa gtagatgtag aaggtaaatc atatacacca caagaaatct
     3841 cagcaatgat ccttcaatat atcaaaggat tcgcagaaga ctatttaggt gaaaaagtcg
     3901 aaaaagcagt tatcactgtg ccagcttact ttaatgatgc acaacgtcaa gcaacaaaag
     3961 atgctggtaa gatcgctggt ctagaagtag aacgtatcgt caatgaacca actgcagctg
     4021 ctcttgccta tggtttagat aaaacagatc gcgacgaaaa aatcttagta ttcgaccttg
     4081 gtggcggtac ttttgacgta tctatccttg aactaggaga cggtgtcttt gatgtattgt
     4141 caacagctgg cgacaaccat ctaggtgggg acgactttga taacaaaatc atcgactaca
     4201 tggtagcaga attcaaaaaa gaaaacagca ttgacttgtc tcaagacaaa atggctttac
     4261 aacgcttgaa agacgctgct gaaaaagcga aaaaagattt atctggcgta tcaagcacac
     4321 aaatcagctt gccatttatc acagcaggag aatctggacc attgcatttg gaaatgacat
     4381 tgacacgtgc aaaatttgat gaattaacag ctgacttagt agaacgtaca aaagtaccag
     4441 tacgtcaagc gttaaaagat gcaggtctgt cacaatcaga aatcgacgaa gttattttag
     4501 tcggtggatc tactcgtatc cctgcagttg tagaagcagt acgtaaagaa acaggaaaag
     4561 aaccaaacaa atcagttaac cctgatgaag tagttgctat gggtgctgct atccaaggtg
     4621 gcgtgatcac tggtgatgtg aaagatgttg ttttacttga cgttacacca ttgtcattag
     4681 gtatcgaaac aatgggtgga gtgttcacta aattgattga ccgcaataca acgattccaa
     4741 caagtaaatc acaagtattc tcaactgcgg cagataatca accagcagta gacatccatg
     4801 tattacaagg tgaacgccca atggctgctg acaacaaaac tctaggaaga ttccaattga
     4861 cagatattcc agcagctcct cgtggtattc cacaaatcga agtaacattc gatattgata
     4921 aaaacgggat tgtaaatgta tctgcgaaag atctaggtac acaaaaagaa caaaaaatca
     4981 cgatcaaatc ttcttcaggt ctaacagatg aagaaatcga acgtatggtg aaagatgcag
     5041 aagcaaacgc tgaagcagat aaagctcgta aagaggaagt tgacctacgt aatgatgtag
     5101 acgcattgct attctctgtg gacaaaacat tgaaagaatt agaaggcaaa gtggacgaag
     5161 aagaagtgaa aaaagcagaa acagctcgtg acgaattaaa agccgctgtt gaagcaaaca
     5221 acatcgagga aatgaaaact aaacgcgatg cattgaacga aatcgttcaa aacttgactg
     5281 ttaaactata cgaacaagct gcacaacaac aagctcaaga aaatccagaa gcagctcaag
     5341 gcggagcaga tgatgtcgtt gatgctgatt ttgaagaagt cgatggcgac gataaataat
     5401 cagttcactg aatttcgaga gaaggccaaa gttgctactt tggctttttc ttttgaaaaa
     5461 tagtttgaag tatcggtttt tgtttcaaac aaaatatgat atgataacaa gcgacgtgaa
     5521 aatgatgaga accaatagga ggaaagccta tggcaacaaa acgtgattat tatgaagtct
     5581 tagggctctc aaaaggagca tcagaagatg aaataaagaa agcttatcgt aagctttcga
     5641 aaaaatacca tcctgacatc aataaagaac ctgatgcgga agaaaaattc aaagaagtat
     5701 cagaagcata tgagatttta agtgacccgc aaaaacgcgc tgcatacgac caatacgggc
     5761 atgcaggcac agatccaaat tacggtgctg gtggagctgg tggcggattt ggtggattcg
     5821 gcggcttctc tggtggcggc ttcggtggtt ttgaagatat cttcgactct ttctttggag
     5881 gaggcggtcg atcagtcgat ccaaatgcgc ctcgtcaagg tgctgatttg caatatacgg
     5941 tcgacttgtc ttttgaagaa gcgatttttg gtgtagaaaa agaaatcaag tataatcggg
     6001 aagaaatctg tcatacttgt ggtggaaacg gtgcaaaacc aggcacacaa ccaactactt
     6061 gtcataaatg tcatggtgca ggtacgatca atgtagaacg ccaaacgcca cttggacgag
     6121 tgatgagccg tcaaacttgt gatgtttgtc atggcacagg gaaagaaatc aaagaacctt
     6181 gtccaacctg tcatggctca ggacatgaga agaaagcaca ttcagttaaa gtgaatgtac
     6241 cagctggtgt agaagaagga caacaaatgc gtttagctgg tcaaggagaa gctggagaaa
     6301 acggcggacc atttggtgat ttgtatgtgg tctttcgtgt agaagaaagt gatatctttg
     6361 atcgtgaagg ttctgaaatt tactatgaat tgcctttaag ttttgtacaa gcagctttgg
     6421 gggatgaagt ccaagtacca actgtccatg gagatgtgaa attgaagatc cctgctggaa
     6481 cacagacagg tacgaacttc cgtttacgtg gaaaaggcgc tccgaagtta cgtggtggtg
     6541 caacaggcga ccaacatgtc aaagtaaaac tgatcacacc gaaaaatcta aatgaacaac
     6601 aaagagaagc attacgtgca ttcgctgaag ctggcggcat caaagttgaa gaacagcaag
     6661 aagacggttt cttcgataag tttaaagatg cttttggcgg gaaaaagcgg aagtagtttt
     6721 tctagtaaaa atataaagta gacttgccca aattttctat ttctagtaaa aacgctaagt
     6781 cattaataat taaaaaccag acgaacaatc atacgttcgc ctggtttttt tgtagttgtc
     6841 atgtttgtac caacaaaaaa gccagcttta gaataagata atcatcatag tttctgaagg
     6901 tggtatcgac tatgtacttg atatcgtcaa tgaaccgatg gtactttctt atgcgatgga
     6961 acaggtagag cgattgcttg tttgtgcagg agaacaagcg atgcggctgg tacagttaag
     7021 tgaaaaaaca agaaactaaa aatgcccggt cgtcatgaag caaccgggca tttttggttt
     7081 ttattaaata ttcaatactt tattcaagaa atcttttgtc cgttcatttt gcggattttc
     7141 aaagatttgt tgaggcgttc cgtcttctaa aaagtttccg tcgtcaatga acatgacacg
     7201 attggccact tctttagcga agcccatttc gtgcgtaacg ataaccatcg tcataccttg
     7261 ttctgctaac tttttcatga cgttcaagac atcaccgacc atttcggggt cgagtgcaga
     7321 tgtaggctca tcaaaaagca tgatgtcagg gttcatcgct agagcacgag caatagccac
     7381 acgctgtttt tgtccaccag aaagcatttc aggatacata tcttttttat cttctagacc
     7441 aactgtctgc agtaatttga ttgctttttt ctctgcttct tctctagatt ctttcttcag
     7501 gtccatcggt gctaaaatga tgttttccaa aatagaaaga tgagggaata ggttaaagtg
     7561 ctggaatacc attccgatat gttgtctcac caagttgata tctgtgcttt tatccatcaa
     7621 gtgggcgccg tcaataataa tttcaccgtc agaaggttct tctaaacgat tcaagcaacg
     7681 caaaaatgta ctttttcctg aacctgaagg gccaatgata cagacgacat ctccttcgtt
     7741 gatggataca ttgatgtcgt ttaatactgt gttatcgccg tattttttga ctaaatgctc
     7801 aactaagatt ttttctgcca tcttacttca ccttcttttc taaaactttt gctaattttg
     7861 tcaatgctgt gatcaaaatc aagtacatga tcgcaatgat caagtaaaca tacgtacttt
     7921 gtgttgtacg tgcaacaatg atcttaccag tttgcagcaa ttcgactaca ccgattgcgg
     7981 aaatgatcgt tgtgtctttt aatgagataa cgaactgatt gacgaatgat gggatcatga
     8041 tgcgtattgc ttgtggcaaa atgatttttt gcattgtccg attataagaa agacctaaac
     8101 ttctggaagc ttccatttgt ccgacaggaa cggcgttgat cccgcctcga acaatctctg
     8161 cgatataagc acttgcgtta agagtcaagg tgataacacc agcaaggaag tcaggaatcg
     8221 taaatccaac gataccaggc aacccaaaga aaataaagaa cgcaagaacc atcattggaa
     8281 ttcctcggat gatatcgaca taaatactag caattgtgcg aagagcgcgt attggtgcta
     8341 cactgaataa tccaaaaatc acaccgatta ctatagccaa agcaaaggag atcaaagtta
     8401 ggagaattgt tctccaaagt cctttcaaca atgcttgata gttgttttgg ataagtccaa
     8461 gaaatgttga ttcatcagca gtggaagact cactagaagt ttctgaacca tccgctacat
     8521 attctttgat aatcttatcg tattcgcctg ttctttttaa ttctttcaat ccttcattga
     8581 acatattaag aagctcagga ttttgtcctt ttttaacggc aaatgcatat tttccgccag
     8641 attctttttc gatcggtgtt tccaattctt gtccttgttt gatcgcataa ccgattaccg
     8701 gataatcatc catcattgca tcgattgcgc caacttttaa cccgtcgtac aattgatcag
     8761 cagcatcaaa atatttgata gaatagccat attcagattc atggtcgctt aaaaattcag
     8821 cactttcggt accaactttt gctccgactg tttttccttt caaatcttca taagacttga
     8881 tcgaagtatc tccttttttg acagctaatt ggatcccgct atcaaagtaa gaatcagaaa
     8941 aatcatagct tttctttcgt tcatctgtta cagtcattcc ggcaatcatt ccgtctgctt
     9001 gtcctgattc caaagcttgc aaagcagcag agaaaccacg gtagtccatt cggatgttaa
     9061 atccttgcag tttggcgatg ttttccatca attttacatc gatgccttcg tactcgccct
     9121 ttgcgttttg aaattcaaat ggagcaaaag cagagtcact ggcaattacg tatacgtctt
     9181 ttttgggctc tattttttgc attgtctcgc ttgtgcttga ctctgccgtt gtagtgttgt
     9241 cagcttgtgc aacatgacca aacgcaagtg tcacaagtcc tgtcattaag aaaacaaatg
     9301 agtgaaataa atgctttctt ttcataatca acctcctaaa tttccaatat tttattatta
     9361 taggcgaaaa gtgctgaatc agacaagact gtaagctcga aaaaatgaaa aaaatgcgag
     9421 tttctaacat tttatgttat aaaacgtaac atgtataata agataaaaaa gtggcgaaag
     9481 tatgttcggt gtggtataat tttttagtaa ggaatgcctt tgctttcctt ttctttttat
     9541 tggtcgtaaa atgaaaagga atgaatgaaa aggaggacga agaatgattg agagagatac
     9601 ctcccgacac tttgagcttg tctcgaaata ccaaccggct ggtgatcagc ctgaagcgat
     9661 taaccaatta gtggatggcg tagtcggcgg gaaaaaagca caaatcttat taggtgcgac
     9721 tggaacaggg aaaacatata cgatttccaa tcttattgag aaagtgaata aaccaacact
     9781 gattattgct cataataaaa cattggctgg tcagctgtac ggggaattca aggaattttt
     9841 ccctaacaat gctgttgaat attttgtttc ttattatgat tattatcagc ctgaagctta
     9901 tgtgccttca agcgatacgt atatcgaaaa agattcaagt gtaaatgatg agatcgacaa
     9961 attacgacat tcggctacta gttcattatt agaaagaaat gatgtgatcg tcattgcatc
    10021 cgtttcatgt atttttggat taggttcgcc ttttgagtac caaaaacaag tagtttcgat
    10081 tcgacaggga gcagaactag atcgcaatca gttgattcgt gacttagtca gtatccaatt
    10141 cgaaagaaat gatatagatt ttcagcgcgg tcgttttcga gtgcgcggtg atgtcgtgga
    10201 gatttttccg gcttcaagag atgaacgtgc cttacgtgtg gagttttttg gagatgaggt
    10261 cgaacgtatc cgagaagtca atgcactgac aggtgaagtt ttaggagaaa cggaacacgt
    10321 ggcaatcttc ccggcgactc actttgtgac gaatgatgaa catatggagc atgccgtagc
    10381 gaatatcaaa gcggaattag aacagcgttt aactgtcttg cgaaatgaaa acaaacttct
    10441 ggaagctcaa cgtttagaac agcgaaccaa ttacgatatt gaaatgatgt tagaaatggg
    10501 ctacacgtcg ggaattgaaa attattctcg gcatatggac ggacgtaaag aaggtgaacc
    10561 gccttatacg ctattggatt tctttcctga agattttttg atcgtggcgg acgaatcgca
    10621 cgtaaccatg cctcaaatca gaggaatgta taatggtgac cgagcgagaa aacagatgct
    10681 agtagattac ggtttccgtt tgccatctgc tttagacaat cggccacttc ggttagaaga
    10741 gtttgaaaaa catgtcaatc agatcattta tgtctctgcg acgccaggac catatgaaca
    10801 tgaacaaaca gataccgtta tccaacaaat catccgtccg acagggctgc ttgacccagt
    10861 gatcgaagtt cgtccaatca tgggacaaat cgatgatcta gtaggcgaaa taaatgaacg
    10921 agtcgaaaaa gaccagcggg tgtttgtaac gacattgacg aaaaaaatgg cagaagacct
    10981 cacagattat ttcaaagaat tagggatcaa agtcaaatat ttgcatagtg atatcaaaac
    11041 gctggaacga acagaaatca ttcgagatct tcgattaggt gaatttgatg tgttagttgg
    11101 aatcaatttg cttcgtgaag gattagacgt acctgaagtt tcccttgttg ctatcttgga
    11161 tgcggataaa gaaggctttt tacgtagtga acgttcctta gtccaaacga ttggacgagc
    11221 tgctcggaat gaagaaggta aagtaatcat gtacgcagac aaagtaacag attctatgcg
    11281 tctagcgatg gatgaaacgt caagacgtcg tacgattcag cagaagtaca atgaagaaca
    11341 tgggatcgtt ccaaaaacga tcattaaaga aatccgagat ttgatttcta ttacgaaaga
    11401 atcggaagat gatacaaaag aagcggtaca agtctcgtac gaagaaatga cgaaggaaga
    11461 aaaagacacg ttattgatga aattagaaaa agaaatgaaa gatgcggcaa aagctttgga
    11521 ttttgaaaca gccgcgaatg tgcgagatat gattttagaa ttaaaagctt caaagtagga
    11581 ggacaacatg gctaacgata aaattacgat tcatggtgca cgtgcccata acttgaaaaa
    11641 tatagatgta acgattccac gagataaatt cgtggtcgtt acaggtttgt ccggttctgg
    11701 aaaaagttcc ctagcatttg atacgctgta cgctgaaggg cagcggagat acgtagagag
    11761 cctttctgct tatgctcgtc agtttttagg acaaatggat aagccggacg tagatagcat
    11821 cgacggattg agtccggcga tttctattga tcagaaaaca acaagtaaaa atcctcggtc
    11881 gacagtaggt actgtgacgg aaatcaatga ctatttacgc ttgttgtttg cacgtgtagg
    11941 acatccgatt tgtcccaacg atcatatcga gattaccagt caatcagtag atcaaatggc
    12001 agatcaagta atggctttgc ctgagcgcac aaagatccaa gtgttggcac cgatcgtggt
    12061 aaagaaaaaa ggacaacata aaaaaatatt cgaacgaatc caaaaagagg gatacgtccg
    12121 cgttcgagtc gatggtgaga tctatgattt aagtgaagtg cctgagctgg agaaaaacaa
    12181 aaaacatgat atcgcgatcg tcatcgaccg gatcattgtg aaagaaggga ttcgttctcg
    12241 cctatttgat tctttagagg cggccctgcg attagcagac ggctatgctc tggttgacgt
    12301 gatcggtgaa gaagaaatgt tgtttagcga acactatgcc tgtccttatt gcggatttac
    12361 tgttggcgaa ctagaaccaa gactgttctc attcaatgcg ccttttggag cttgtccaga
    12421 ctgtgatggt ttaggtgtca aattagaagt cgatcaagat ttagtgattc cagatagaac
    12481 gaagacactt agtcaaggtg ctatcgttcc atggaatcca atcagctctc agtattatcc
    12541 tcaaatgtta gcgcaggctt gtgaaagttt cggtatcgat ttagatacac cattcgaaga
    12601 attaccggaa gagcatcagg agatcatctt gaatgggaca gatgatcttt tccatttcca
    12661 ttatgaaaat gattttggtg gcgttcgcga tgtagaaacg actttcgaag gggtattgaa
    12721 gaatatcgag cgtcgttatc atgatacgaa cagtgatttc acgcgtgagc aaatgcgtct
    12781 ttatatgaca gaattaactt gtcagacatg taaaggttat cgattgaatc cccaagctct
    12841 atctgttcaa atcaatggaa cgaatatcgg gcaaacaaat gagttgtcta ttcaaaaagc
    12901 agtagatttc ttctcaaact taacgttatc tgaacaagag aaagtaattg ccaaaccgat
    12961 tttaaaagaa gtaaatgaca gactttcatt cttggaaaat gtgggactag actatctaac
    13021 tttgagtcgc gcttccggaa cattatctgg gggagaagcg cagcgaatca gattggctac
    13081 acaaattgga tcgaatcttt ctggagtttt gtatatttta gatgaaccat cgatcggatt
    13141 gcatcaacgc gacaacgatc gactgattgg ctcattaaag aaaatgcgtg atcttggcaa
    13201 caccctcgtt gtagtcgaac atgatgaaga tacgatgtgg gcagcagatt atttgattga
    13261 cgtcggacct ggggccggag aacaaggcgg acaaatcgtg gctgcaggca ctcctgaaga
    13321 agtagctaat gatgaaaatt ctttgacagg gaaatatctt tcagggaaaa aatcaatccc
    13381 tgtaccaaaa gaacgccgta aaggtaatgg gaaagcaatc aagatcactg gtgcttcaga
    13441 aaacaatctg aaaaatatta gtgttgaatt tccattagga gaatttgtag cagtcacagg
    13501 tgtgtctggt tctggaaaga gtacattggt caatagtatc ttaaagaaat cgttagcgca
    13561 aaaattaaat aagaattctg ctaagccagg taaattcaag acaatttccg gctacgaaag
    13621 tatcgaaaag atcatcgata tcgatcaaag cccaatcggc cggacgccga gaagtaatcc
    13681 agcgacttat acaagtgtat ttgatgatat ccgtgggtta tttgctcaaa cgaacgaggc
    13741 aaaaatgcgg ggttataaga aagggcgttt tagtttcaac gtaaaaggcg gtcgttgtga
    13801 agcttgtcgc ggggatggaa ttattaagat cgaaatgcac tttttgcctg atgtctatgt
    13861 tccttgtgaa gtatgtcatg gtaaacgata taattctgaa acattagaag tgcattacaa
    13921 aggaaaaagc attgctgata ttttggaaat gacagtagag gatgctgtag aattcttcaa
    13981 gcacattcca aagattcatc gcaaactgca aacgattgtt gatgttggct taggttatgt
    14041 gacgatgggg caaccagcaa cgacattgtc cggtggtgag gcacaacgga tgaaacttgc
    14101 cagtgaattg cacaaaatct ctaatggaaa gaatttctat atactagatg aaccaacgac
    14161 aggacttcat agcgatgaca tcgcccgctt gttgcatgta ttacaaagat tagtagatgc
    14221 tggtaacaca gttttagtga ttgaacacaa tctagatgta atcaaaacag cagattatat
    14281 cattgattta ggaccagaag gtggagaagg tggaggaacg atccttgcga ctggaacacc
    14341 agaagaaatc attaacgtaa aagaaagtta tacaggtcac tatttgaaaa aaataatggt
    14401 ataaagagga tagatagctg agatagttaa aaaaacataa aaaaatgaat ttttgcttgt
    14461 tttattaata tcagtgttct agaatcatta catggtagtt ctttctacac tgattggtat
    14521 agataaaaac ttcctataag ttcaatccgt tcaaaaaaga attgagcaat cggaggagtt
    14581 tcctttgatt gcttttttct ttatggtatg atattccttg tgtagttgga aaagaaaaac
    14641 aagagattat gagtaagtga gagataatga tgaacagtat tgaaaatgaa gaatttatcc
    14701 ctatattatt aggaagcgac atgaacgttt atggtatggc acgttctttt aatgaagcat
    14761 acggcaaaat ctgccaagca tatgcttcgg atcagttagc ccctacccgc tacagtaaaa
    14821 ttgtcaatgt agaagttatt ccaggatttg ataaagaccc tgtatttatc gagacgatgc
    14881 ttcgtttggc aaaagaaaga tattctgata agagtaaaaa atatcttttg attgcttgtg
    14941 gtgatggata tgcagaattg atctcccaac ataaacaaga gctgtcagaa tattttatct
    15001 gtccatatat tgattattct ttatttgaaa ggctgatcaa taaagtaagc ttttatgaag
    15061 tttgtgaaga atatgatctt ccgtatccta aaacattgat cgtcagagaa gaaatgttag
    15121 taaatggaca tttggaacag gaattgcctt ttgaattccc agttgctttg aaacctgcaa
    15181 acagtgtgga gtatctttct gtccaatttg aaggaagaaa aaaagctttt attttagaga
    15241 ctagagaaga gttcgattta attttaggcc ggatatacga agccggttat aaaagtgaaa
    15301 tgattgtaca agattttatc ccgggtgatg atagtaatat gcgtgtgcta aatgcgtatg
    15361 tagatgaaga tcatcaagtt cggatgatgt gccttggtca tccactatta gaagatccta
    15421 caccagcatc gattggtaat tacgttgtga tcatgccaga ttataacgaa aaaatctatc
    15481 aaaccattaa ggcatttttg gaaaaaatcg aatatacagg ctttgccaat ttcgatatga
    15541 agtatgatcc tagagatgga gaatataaat tatttgaaat caatttgaga caaggtcgta
    15601 gcagtttctt tgtgacattg aacggcttga atctggctcg ttttgtcaca gaagatcgag
    15661 tgttcaacaa accctttgtt gaaacgacct atggaacgaa tcaatcagat aaagcaagat
    15721 tatggatggg tgtgcccaaa aaaatctttt tagaatatgc tcgggaaaat gaagacaaaa
    15781 aactagctga gcaaatgatc aaagaaaatc gttacggtac tactgtattt tatgaaaaag
    15841 accgatcaat caagcgctgg ttgttgatga aatatatgtt ccataactat attccaagat
    15901 tcaaaaagta ttttcatgtg aaagaaggct agttcatgga gaattttttc agtattttag
    15961 gcggaatggg cacgatggcg acagaaagtt ttgttcgctt gatcaaccac cgaacgaaag
    16021 ctacaaaaga tcaagaatat ttgaattatg tcttattcaa tcatgcaaca gttcctgatc
    16081 gtacagctta tattttagat cgatcagaag aaaatccaat gccgtttctg ctggatgata
    16141 ttgagaaaca aaatttattg cgaccaaact tcattgtgtt gacttgtaac accgcacact
    16201 acttttttga agagttacaa gcggcaacgg atattcctat cttgcatatg ccaagagaag
    16261 cagcaaatga acttgtccgt cagcatacga cagggagagt agcgattcta ggaacagaag
    16321 gaagtatgaa agctggaatc tatgaacgcg aagtcaaaaa tcttggtttc gaaacagtga
    16381 ttcctgatac tgcgcttcaa gaaaaaatca attacctgat ctatcatgag atcaaggaat
    16441 cggattattt gaatcaagag ctctattacg aaatcttaga ggaagcggta gagcgcctaa
    16501 actgtgaaaa agtcatccta ggttgcacag aattatcttt gatgcatgaa tttgctgaag
    16561 ataatcatta cccagtgatc gatgctcaat ctattttagc ggatcgaacg atcgaacgtg
    16621 cattagctga aagaagcgag gcattggata cagcatcgga aaagtaaaaa aattggattt
    16681 ggggcaaata tcctaaaaga tatttggtcc aaatcttttt gtaaaagaaa aagaaaactg
    16741 gtaatgatac gcccgggatg atataatttt attatgcaca atgtgagagg ggttcgtaaa
    16801 tatgacagat aatttgcaat tagtcatcat aacaggtatg agtggtgcag ggaaaacagt
    16861 agctatccaa agttttgaag atatgggcta tttttgtatc gataatatgc cgcctcgatt
    16921 gattccaaaa ttctgggaat tgatcaaaga atcaggtaaa gtcacaaaaa tggctttagg
    16981 agtggatttg cgttctcgtt ccttctttga agaaatccaa aacatgctga tcgaaatcga
    17041 aaatacgaac ttcatcgaca cttctatctt atttttagat gcttcagacg aagaactagt
    17101 ggctcgctac aaagaaacaa gacgagcaca tccaatggcg atggacggtt tgatcactga
    17161 agggatacgt aaagaacggg cgatactaga cgacttgaaa gcccaagctt ccgtgatcat
    17221 tgatacgaca acattgacac cacgacaatt acgtgaaaaa atcaatgagt cattcaaatc
    17281 aaagggagac caaggatttc ggatcgaatt tgtatcattt ggttttaaat acgggttgcc
    17341 gatcgattcg gatattgtga tggatgtgcg ttttttgccc aaccctcatt atatcccgga
    17401 actaagacca ttgaccggaa aagatcagcc agtctatgat tatgtgatgt cctttgatga
    17461 aacgcaatca ttctaccata aatttttgga tcttctggaa actgtattgc ctggatacat
    17521 taaagaagga aaaagcagct tgacagttgc aatcggctgt acaggcggtc aacaccgttc
    17581 tgtggcattg actgaacgtt tggcaaatgc tttagggaaa aactataaag tcaatgtgac
    17641 acatcgggac aaggataaaa ggaaagagac ggtgaaccgg tcgtgaaaat gaaaacctac
    17701 cggataagaa aaccaaagat cgtagtggtc ggcggtggca ctggtctgcc agttatcctg
    17761 aaaagtttac gaaatcaagg agcagatatc acagcagtag tgactgttgc agatgatggt
    17821 ggaagcagcg gtgcaatcag agaatcgatc gctatggctc ctcctggtga cttgagaaat
    17881 gtcttagtgg cgttatcgga tatgccacaa ttctatgaag atattttcca ataccgtttc
    17941 aaaaaggaag atcagttttt agccaatcat acgatcggta atctgatcat tgccgcagtt
    18001 tccgagatgc ggggaagcac ttatgaggcg attcaattat tagcaaaaat gatgcatgta
    18061 gatggacatg tttatccttc ttcagaaact cctttgacct tgcatgctgt ttttaaagac
    18121 ggaacgactg cagtaggaga atctaaaatc gcgatcaacc gtaaaaccat tgaccgtgtg
    18181 tatgtctgca acacaaatga taaaagtgaa gccaaagcgg ctcgaaaagt cgtttcagca
    18241 attatggacg ccgacatggt cgttttggga cccggaagtt tattcaccag tatccttcca
    18301 aatttagtga taccagaaat cggcgaagca atgctcaaga caactgctga aaccgtctat
    18361 atctgtaata tcatgaccca aaaaggggaa acggaacatt tcacagatgc cgatcatata
    18421 cgtgttttac acaagcattt gaatcagcct tttatcgata cagtattggt caatacggaa
    18481 aaagtgcctg aagattatat ggacccagag atctatgatg agtatctagt acaagtcgca
    18541 cacgacttca acggattgag aaatgaagga tgtcgagtga tttccaccga ctttttagaa
    18601 ttgaaagatg gtggtgtctt ccacgatgga gacaaagtag tagaagaatt gttccgttta
    18661 gttttcggtt caaaatatta agcaatttat tgttggagaa aggaggagaa taggatgtca
    18721 tttgctgcag atgtgaagaa agaactaact ggtttggaag ttcatcgaga acatgcgaaa
    18781 gcggaattag ctgctttgct gcgaatgaac ggctctttag gtattgtgaa ccagcagttt
    18841 attttgaacg tccaaacaga aaatgctgca attgccagac gtatctattc tcttttgaaa
    18901 gatcattatc aggtacgaag tgagctactc gttcgtaaaa aaatgaaatt aaaaaagaac
    18961 aatgtctata tcgtccggtt gaagcaaggc acaaaagatg tgttgatgga tcttgatatc
    19021 atggatggta tgatgttcaa tggacacgtt tcggacgaga ttatgggaaa ttcgcaaaaa
    19081 atgcgttctt acttacgtgg tgcttttatg gcctcaggtt cagtcaataa tccggaaacg
    19141 agtcgttatc atttggaaat attttctatt tatgaacagc ataaccaaga catttgcgat
    19201 atgttaaatt attacggtct acatgctcga acgcttgaac ggcgcaatgg ctatatttcg
    19261 tatttaaaag gagcagaaaa aattgctgat ttcttaacat tgattggtgc aaccaactca
    19321 atgctccgat ttgaagatgt tcgaatcgta cgggatatga gaaattcggt caatcgtcta
    19381 gtcaattgtg agacagcaaa tctgaacaag acgattgatg cggcttctaa acaaatcgaa
    19441 aacatcgaat tgatcgaagc aaaggtcggt ctacatgccc ttcctgaaaa acttcaggaa
    19501 atcgcagaac ttcggcttca acatccagag gtcagcttga aagaattagg ggaaatgatt
    19561 ccctcaggtg caatatcaaa atcgggaatt aatcaccgaa ttcgcaaaat aaatgaattt
    19621 gcggaaaaac tgcgtgaaga agcagtttaa tccaaagaaa aaactgatac atgaagcagg
    19681 agatgcgaat gtatcagttt tttttaaaag ctatacttgc ttttcttccg tcaaactata
    19741 attccaactt tactggagta acataatgtt gaaaggcttc tattgttttt taatacaatc
    19801 gtataaaaag cggggagaca actgaatgaa gattttagtt ttatacataa acttttgctt
    19861 aatatagaag tcctgatata gataggatca agtatttatc tataactgga gattaattaa
    19921 aaactggaag gaactgctcg atcaaccttg agaactgttt ttcttacttc tggtcaagat
    19981 tctcgtggtt ggtggataag gatggttgat aagttcattg gattagctag tcgaaaacaa
    20041 ccaggtgtaa ttaatgtaga agttttaaca aggggatgga gtgatttatt cagtgataat
    20101 cataagttca taaatagtaa ttgggatgta tggttggtat acgttatacc tgaacttgaa
    20161 ctatcaagat ttggtttaga atttgagaat cgatgtttat aaagggaggg caaaaaatgt
    20221 gcttttttac aacttttact atcattatga ttgcagtact ttttattttt ctagatatag
    20281 caaagcgaaa tacagcattt ttattatatc gggttttact tcgagcaggt ctcataacat
    20341 ttatatcgat cgttggattt tttctattca cagttattgt atttatttgg agaactacag
    20401 caccaccatt gccagagatt acatatggag aatttccgtt tagtcttgaa tatgaaatag
    20461 atgaggaaag aattttgatt gaagatacgg tgattgcaga atttatagga agttttagag
    20521 ggaatgtgac aactcaacct agacgaagtt ggagtataaa acttttaaat aatagtggag
    20581 agttgaaagc atttcattat gaattcttaa taatagaaag tgaagatgta acaatcactt
    20641 taagtagtgg ctctgctgga tattttatga atgatggtgg aagcaagcca cagctcacag
    20701 tccgtgatcg tattgcaggt tttatgcatt tttctcacta tgacttagaa gaagctcgta
    20761 aaatacttgc agaatatggg atcacattaa tcaattggga atatgcgcca ccgataagaa
    20821 attttttcaa tgattattat tagtcttata aaaaacagaa tgtaaaattt atgtgcgcga
    20881 gtatttagtt ttccattttt tgtttgatag atatatagca ttgttgaagc atgggatagt
    20941 ggtgccaatt ttatcaatga ggttggtcag gcagttaaca taattggtga tgctataaat
    21001 ccatttaatt ggaattggag gaattaggtg tggaaacagt gagattatgg atattcatga
    21061 aaaattttcg ttcggctttg agatatgtta ttctggaatc aaaatcaggt tattatctga
    21121 ttgacacaga tcactatact ttttggggag gctacctttt tccgattttt aattggataa
    21181 tcccccataa ggttatgaaa attaacttgc ctgcagaaga aataaataga ttaatactca
    21241 acaaagattt acaaaggaaa agagagaaag aacattctaa tgttgcttgg ataggtccag
    21301 catttgcagt agtgacttct tcaattgtcg gtcctatgct agagagtatt tatagtgatt
    21361 tttctattgg aatacgttat ttatttgtta ctttaagtat agctgttctt ttgtgcataa
    21421 agggttatgt tagcaagaag aataaagata ttttaacact aatcggtgta aacaatttat
    21481 ctagtactaa aattatactg tttcctgaat ctataaaaga aggatcaagg ttgttgataa
    21541 tttttataat gcttatactg ttttctgttt tatttggggt gatgtttata ga
//
LOCUS       DAPBAB010000043        21277 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPBAB010000043 DAPBAB010000000
VERSION     DAPBAB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.043"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            130..504
                     /locus_tag="RD444_001925"
     CDS             130..504
                     /locus_tag="RD444_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7549296.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(618..1247)
                     /locus_tag="RD444_001926"
     CDS             complement(618..1247)
                     /locus_tag="RD444_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7549297.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1381..1453)
                     /locus_tag="RD444_001927"
     tRNA            complement(1381..1453)
                     /locus_tag="RD444_001927"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Thr,seq:ggt)
     gene            1667..1740
                     /locus_tag="RD444_001928"
     tRNA            1667..1740
                     /locus_tag="RD444_001928"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1701..1703,aa:Asn,seq:gtt)
     gene            complement(1878..2042)
                     /locus_tag="RD444_001929"
     CDS             complement(1878..2042)
                     /locus_tag="RD444_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549298.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2337..3665
                     /locus_tag="RD444_001930"
     CDS             2337..3665
                     /locus_tag="RD444_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDT7549299.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3867..4700
                     /locus_tag="RD444_001931"
     CDS             3867..4700
                     /locus_tag="RD444_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDT7549300.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4716..5453
                     /locus_tag="RD444_001932"
     CDS             4716..5453
                     /locus_tag="RD444_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDT7549301.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5608..6576)
                     /locus_tag="RD444_001933"
     CDS             complement(5608..6576)
                     /locus_tag="RD444_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDT7549302.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6808..7641
                     /locus_tag="RD444_001934"
     CDS             6808..7641
                     /locus_tag="RD444_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDT7549303.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7675..8514
                     /locus_tag="RD444_001935"
     CDS             7675..8514
                     /locus_tag="RD444_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7549304.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8540..9100
                     /locus_tag="RD444_001936"
     CDS             8540..9100
                     /locus_tag="RD444_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDT7549305.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9299..11020
                     /locus_tag="RD444_001937"
     CDS             9299..11020
                     /locus_tag="RD444_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDT7549306.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11185..12327
                     /locus_tag="RD444_001938"
     CDS             11185..12327
                     /locus_tag="RD444_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7549307.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12343..13554
                     /gene="thiI"
                     /locus_tag="RD444_001939"
     CDS             12343..13554
                     /gene="thiI"
                     /locus_tag="RD444_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDT7549308.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14127..14774
                     /locus_tag="RD444_001940"
     CDS             14127..14774
                     /locus_tag="RD444_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDT7549309.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14903..15119
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15167..17812
                     /locus_tag="RD444_001941"
     CDS             15167..17812
                     /locus_tag="RD444_001941"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDT7549310.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18122..19435
                     /locus_tag="RD444_001942"
     CDS             18122..19435
                     /locus_tag="RD444_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDT7549311.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19425..20078
                     /locus_tag="RD444_001943"
     CDS             19425..20078
                     /locus_tag="RD444_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7549312.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20133..20825
                     /gene="radC"
                     /locus_tag="RD444_001944"
     CDS             20133..20825
                     /gene="radC"
                     /locus_tag="RD444_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDT7549313.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20975..21175
                     /locus_tag="RD444_001945"
     CDS             20975..21175
                     /locus_tag="RD444_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7549314.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         7184 a         3549 c         4445 g         6099 t
ORIGIN      
        1 tacactctga gatgtaagaa atatcataag atatttctta catctttttg ttttgagcag
       61 gactttagat aattttatta atatatgcta gcatgctttt gtaagaaaaa gaaagagggc
      121 gtattagtta tgtatcttaa aattacggaa caagcacaag aaagattaca aaaatatatt
      181 gatgaaggcg caacagttat attggattta gatgatggcg ttggggaata ttctaaaatg
      241 ggtgtttgct ctttagatac tagttttcgt ttacttcttc ttgataaaga acagagaaaa
      301 aatgattata aactccaatt agatagtgat attggggatg tttatataaa agattattca
      361 aaaatgtaca tggatgaaca tatgaccctt tctttagatc cacgtctagg tgtatttact
      421 cttgaaagcc caagtggttc tttagacagt catgttcaat tagtagatct acgaccagaa
      481 aaattctctg gaataaaaca ataactttct ctctattgtc cataaaacta gtctcaatca
      541 agctgtattg aaaaaacaag acaacattct taccatggta aataaaaaga cgacacattt
      601 tgtcgtcttt ttttaaatta actattttcg ataaaagatc gcatagcttg tgccattatt
      661 tctggctttt ctctattcaa ttcatgtttt ccgtttttaa taaagagtag ccggccattc
      721 gttgaaagtt tatataaacg ttttgctgct ggtttattcg catgatcttt ggtaccgcat
      781 aaaatcaatt taggaagtcg tgatgtacga agctgttttt tgcagtctaa tgtttcaagt
      841 gaacgaagta gggtaagggt ttgtttttta gatagaccta atttttcaaa tatcttagaa
      901 ggtacgcagt aaaaataaat attctggaaa gtaagcaata agcgattagg agactcaaat
      961 tgtggagcga ctaaaacaac cttttttaaa tatggagaag gagttgcaag atagtttaaa
     1021 gcaataacag cgcctaaaga taatccacat aaaataaaag gttcctttat ctgattcaat
     1081 tgtacttcga tgtatttggt taagtcgtct aaagtagcag tgtctgtgct gtttaaatga
     1141 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
     1201 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
     1261 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
     1321 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
     1381 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
     1441 gagctaaagc ggcattatca ttaatacaaa taataggata acgaatatta atgtatttgt
     1501 caacagaatt ttttcgaaaa aatgtgtttc tctaatagga aaacagattt tagaaaaaat
     1561 aaaaataaca aaaaatgcga acggtttctt tgataatata ataaaaaaat tcaaaaaaag
     1621 tattgcgtta tttagaagaa attgatatta tattcatgta cttgattccg gcatagctca
     1681 gttggtagta gcgcatgact gttaatcatg atgtcgtagg ttcgagtcct actgccggag
     1741 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
     1801 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
     1861 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
     1921 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
     1981 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
     2041 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
     2101 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
     2161 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
     2221 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
     2281 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
     2341 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
     2401 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
     2461 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
     2521 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
     2581 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
     2641 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
     2701 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     2761 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     2821 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     2881 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     2941 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     3001 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     3061 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     3121 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     3181 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     3241 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     3301 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     3361 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     3421 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     3481 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     3541 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     3601 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     3661 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     3721 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     3781 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     3841 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     3901 taaatggtaa agtgattcca gttgttggtg gagatgttaa tcagacctat cgtataaaaa
     3961 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     4021 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     4081 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     4141 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     4201 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     4261 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     4321 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     4381 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     4441 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     4501 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     4561 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     4621 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     4681 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     4741 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     4801 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     4861 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     4921 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     4981 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     5041 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     5101 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     5161 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     5221 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     5281 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     5341 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     5401 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     5461 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     5521 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     5581 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     5641 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     5701 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     5761 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     5821 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     5881 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     5941 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     6001 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     6061 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     6121 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     6181 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     6241 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     6301 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     6361 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     6421 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     6481 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     6541 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     6601 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     6661 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     6721 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     6781 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     6841 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     6901 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     6961 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     7021 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     7081 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     7141 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     7201 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     7261 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     7321 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     7381 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     7441 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     7501 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     7561 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     7621 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     7681 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     7741 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     7801 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     7861 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     7921 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     7981 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     8041 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     8101 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     8161 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     8221 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     8281 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     8341 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     8401 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     8461 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     8521 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     8581 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     8641 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     8701 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     8761 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     8821 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     8881 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     8941 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     9001 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     9061 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     9121 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     9181 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     9241 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     9301 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     9361 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     9421 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     9481 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     9541 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     9601 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     9661 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     9721 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     9781 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     9841 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     9901 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     9961 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
    10021 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
    10081 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
    10141 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
    10201 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
    10261 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
    10321 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
    10381 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
    10441 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
    10501 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
    10561 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
    10621 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
    10681 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
    10741 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
    10801 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
    10861 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
    10921 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
    10981 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
    11041 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
    11101 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
    11161 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
    11221 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
    11281 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
    11341 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
    11401 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
    11461 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
    11521 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
    11581 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
    11641 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
    11701 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    11761 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    11821 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    11881 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    11941 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    12001 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    12061 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    12121 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    12181 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    12241 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    12301 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    12361 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    12421 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    12481 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    12541 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    12601 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    12661 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    12721 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    12781 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    12841 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    12901 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    12961 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    13021 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    13081 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    13141 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    13201 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    13261 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    13321 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    13381 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    13441 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    13501 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    13561 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    13621 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    13681 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    13741 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    13801 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    13861 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttgcgaa
    13921 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
    13981 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
    14041 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
    14101 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
    14161 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
    14221 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
    14281 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
    14341 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
    14401 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
    14461 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
    14521 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
    14581 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
    14641 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
    14701 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
    14761 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
    14821 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
    14881 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
    14941 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
    15001 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
    15061 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
    15121 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
    15181 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
    15241 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
    15301 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
    15361 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
    15421 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
    15481 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
    15541 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
    15601 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
    15661 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
    15721 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
    15781 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
    15841 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
    15901 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
    15961 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
    16021 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
    16081 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
    16141 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
    16201 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
    16261 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
    16321 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
    16381 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
    16441 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
    16501 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
    16561 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
    16621 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
    16681 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
    16741 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
    16801 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
    16861 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
    16921 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
    16981 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
    17041 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
    17101 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
    17161 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
    17221 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
    17281 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
    17341 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
    17401 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
    17461 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
    17521 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
    17581 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
    17641 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
    17701 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
    17761 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
    17821 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
    17881 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
    17941 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
    18001 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
    18061 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
    18121 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
    18181 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
    18241 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
    18301 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
    18361 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
    18421 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
    18481 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
    18541 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
    18601 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
    18661 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
    18721 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
    18781 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
    18841 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
    18901 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
    18961 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
    19021 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
    19081 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
    19141 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
    19201 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
    19261 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
    19321 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
    19381 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
    19441 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
    19501 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
    19561 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
    19621 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
    19681 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
    19741 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
    19801 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
    19861 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
    19921 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
    19981 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
    20041 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
    20101 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
    20161 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
    20221 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
    20281 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
    20341 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
    20401 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
    20461 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
    20521 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
    20581 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
    20641 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tattccatcg cagcaggata
    20701 tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact gtactggacc
    20761 acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg atggaagaga
    20821 agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg taattttgtt
    20881 tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat gttacaattt
    20941 aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag taaaatggtt
    21001 caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg tattcgttca
    21061 cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag cagtgacttt
    21121 cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag cttaatttta
    21181 tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc gagtaacaaa
    21241 aatacctgcc acaagagaca atttctcttg tggcagg
//
LOCUS       DAPBAB010000044        20441 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPBAB010000044 DAPBAB010000000
VERSION     DAPBAB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.046"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            132..452
                     /locus_tag="RD444_001946"
     CDS             132..452
                     /locus_tag="RD444_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549315.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            904..1104
                     /locus_tag="RD444_001947"
     CDS             904..1104
                     /locus_tag="RD444_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7549316.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1251..4040
                     /locus_tag="RD444_001948"
     CDS             1251..4040
                     /locus_tag="RD444_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDT7549317.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4087..4614
                     /locus_tag="RD444_001949"
     CDS             4087..4614
                     /locus_tag="RD444_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDT7549318.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4635..5825
                     /locus_tag="RD444_001950"
     CDS             4635..5825
                     /locus_tag="RD444_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7549319.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5865..7163
                     /gene="asnS"
                     /locus_tag="RD444_001951"
     CDS             5865..7163
                     /gene="asnS"
                     /locus_tag="RD444_001951"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDT7549320.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8216..8890
                     /locus_tag="RD444_001952"
     CDS             8216..8890
                     /locus_tag="RD444_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7549321.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8887..9228
                     /locus_tag="RD444_001953"
     CDS             8887..9228
                     /locus_tag="RD444_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549322.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9258..9617
                     /locus_tag="RD444_001954"
     CDS             9258..9617
                     /locus_tag="RD444_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDT7549323.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10141..12222
                     /gene="cadA"
                     /locus_tag="RD444_001955"
     CDS             10141..12222
                     /gene="cadA"
                     /locus_tag="RD444_001955"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDT7549324.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12533..13999
                     /locus_tag="RD444_001956"
     CDS             12533..13999
                     /locus_tag="RD444_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7549325.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14614..15153
                     /locus_tag="RD444_001957"
     CDS             14614..15153
                     /locus_tag="RD444_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HDT7549326.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15227..15667
                     /locus_tag="RD444_001958"
     CDS             15227..15667
                     /locus_tag="RD444_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDT7549327.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15680..15889
                     /gene="copZ"
                     /locus_tag="RD444_001959"
     CDS             15680..15889
                     /gene="copZ"
                     /locus_tag="RD444_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDT7549328.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15889..18075
                     /locus_tag="RD444_001960"
     CDS             15889..18075
                     /locus_tag="RD444_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDT7549329.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18095..20266
                     /gene="copB"
                     /locus_tag="RD444_001961"
     CDS             18095..20266
                     /gene="copB"
                     /locus_tag="RD444_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDT7549330.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6819 a         3553 c         4434 g         5635 t
ORIGIN      
        1 gttgtgacgt ggtcacgacc tctttttgac gattatgata caaaaaacat catttatgaa
       61 caaaatgtga ataacgggcg ttcttgagat atgattttaa ttgggaaaaa cttatcgata
      121 aggaggtctt tatgaaaaaa agactgttat ggtgtttgct gtttctatcc ctaagtttaa
      181 caagtcagcc agtttttgct gaagaaacac agcggtctcc tattacaggc acacgtctgt
      241 acaaaaatgt ttatgtacag acaacagatc ttgattctgc gattcctgaa acagaagaac
      301 ggtctttgca gccaagattt ggttactttt ggtactgtcc gacctgtgat tatgaaagtt
      361 gctggcattt cttacattct acagctgcag cttttgcttt tactcatact cagaaatatg
      421 atggacatca tcctgttatt tatgaggtat agatatgagc aaaaaagaaa aactaatctc
      481 gttgatcgta ttggtttgcc ttgttcttct ggtaactgtc ataggctatc agaaatacca
      541 tgagaacatc agacctacta aagaatcttc cccagcctcg accgttgaaa cagaaccgat
      601 aagtagtcag aaagaataat ttttttagtt taaagaagaa catcagagca aaaaaacacc
      661 aactgaacaa tatgtcgttt attttaaaaa aagttcggtt tttcacttta tgtaatcgct
      721 gagaaaactt tttttaataa aaaaagtcga aaaactctag cttttttgta aacggtatga
      781 tatactatag gagtagtttt tgttcggtaa tctgtggtag cttgtttcaa ataaacatct
      841 tccagatata caccattcaa gtaattgcaa aatatgtgtt gatacacaag gaggattttt
      901 attatggaaa caggtacagt aaaatggttt aactcagaaa aaggatttgg attcatcact
      961 gcagaaaacg gaaacgatgt attcgttcat ttctcagcta tccaaggcga cggattcaaa
     1021 actttagaag aaggccaagc agtaactttc gatgttgaag aaggccaacg cggacctcaa
     1081 gcaacaaacg ttactaaagc ttaattaacc tatactaaaa aataaacgcc cagacatttg
     1141 cgtgtctggg cgtttttgtc tattttcttt aaaaagcgat gagagtattt ttctgcactt
     1201 tgtagtataa tttcaaaaga acattagagg caggagaaga ggaagatatt ttgaaaccaa
     1261 gaacttatgc agtcgttgat atcgaaacga ctgggacgaa tccaaaagaa gatcggatca
     1321 tccaatttgg ctgcgtcatg attgaatcag gtcggatcac ttcacgtttt tcgatcgata
     1381 ttcatccagg gcggaagatt tcaaaacaaa tccagcattt aacagggatc acgaatcaac
     1441 gcgtccaaaa agcgccttat ttcgaagatg tcgcccaaac gatttacaat cttttggcag
     1501 acaccatatt tgtcgcgcat aatatttatt ttgattataa ttttttgaac catgaactca
     1561 tgcgatgcgg acttcctagt ttgaagatac caggcatcga tacagtagaa cttgcacaaa
     1621 ttttcttgcc gacggaacca agctttcgct tagctgattt gtcagaaagc cttggattta
     1681 ttcatgagaa tcctcatcag gcagatagtg atgccgaagt gacgggtcaa cttcttttgc
     1741 tgatcgaaga acggatgaga aaattgccaa tcatcacgtt agagcagatt gcgcgattaa
     1801 gtagacacac aggtatggat acgagtcgtt tcatttatca tgtgattgaa gaaatgaaag
     1861 aaaaaccaga gccacttgat ccctcattag aaatcgtcga tgggctagct ttgcagaaaa
     1921 aagaagtgga actattcaca tccgtccatt atggcgagcg gacatatcca agaaaaaaac
     1981 aagcaaaaga aaaaatgttt ggtaaaacac tgatgtatcg aaaagaacag aacagactga
     2041 tgaatgcggt ctacgaccat ttcacaaaag atgaatcaaa agatttgatg atcgaagcag
     2101 ctacaggaat gggtaaaaca atcggttatc tgctgccatt gagttatttg gctacacctg
     2161 aaaaacccgc tgtcatcagt accgtttcgc tagttttgca gcagcagatc atagaaaaag
     2221 acattccatt gctgaatcaa ttgctagatc agcccataca acctgtaatt atcaaaagct
     2281 atcgccacta tattgattta cagcgtttta aaggcacact tgtggaaccg ccagaacaaa
     2341 aacaatatgc tttgtatcaa atggcggtct tagtttggct gacgcaaacg aagaccggtg
     2401 atttggatga attacattta acaaatttga accattcatt ctttgcagat atcgcgcaca
     2461 gaggtattgg atttttagca agaaatcagt cattttacga acaagacttt gttcgtttct
     2521 tgcaaaagaa aatcaggcaa agtaattttt tgattgtcaa tcatgcgttt ttaatacaag
     2581 aaacacagcg aaaagaacca ttgcttccag ctagtccatt tttgttgatc gatgaagtac
     2641 atcatttgcc ggaaatcgct gaaaaagtaa atgacaaacg attaagtatt aactatttcc
     2701 ataaacaaat ccagcaatta gaagaaccaa atcagctgtt tgaccgaata caaagctatt
     2761 taccgaaaga acatgaagcc ttacgctggt tatcgattta tcgagaagaa ctttctgcgc
     2821 tatgcgaagc acagatttgt atggcagaag gattaatgag tttagtgagc ggcaagcaac
     2881 ctaacggaca gtatccagaa gaagtcatga tgacaaaaga gatgttggat cagctgccac
     2941 tggaatctgg gacagccatt gatcacctgc agctttttta tcaggagatc cagcagctac
     3001 agtcacaaat gcgtctgact tttgagatta tccaagaaag ctggacaaac cagatgcgga
     3061 tcgatttagc aagtttatat agcttatttg atcaattaga caagcagtac ttgctgatgg
     3121 atcaatggct ggaagactgg cgagatcatt tgatccattg gttcgttcct gggaacacag
     3181 cgcagcagat gatttttcat ctccatgatt ttcaagcggc tatgttgccg tcgactgttt
     3241 ggtatcctcg atatgatcgt attctttata ttggaggaac gctgaagatc ggccctaatc
     3301 gacactatct ctctaagcgg cttggcattg aagcagcacc tcttaaagtg attgcctctc
     3361 cttatgatta tgagaaacag gcaaggatct atgtgccaaa agaggcacct gccgtccatc
     3421 aagtcagcaa tcaagcatat atcgattatt tagagacagc acttacaggt cttatcgaac
     3481 aagaagatcg gccaatactt gtgttattca cttctcatga tgttttgcag aaggtctatc
     3541 agaaaatgca tctgtctatg ctagaaaaag gacgagaaat actagctcaa gggtttggcg
     3601 gaagccggga gaagcttttg aaacgcttca ttttgtcaga aaaaagcgta ttgtttggcg
     3661 ctgacagctt ttgggaaggt gtagatttgc ctggggatgc tttgcagatc gttgtagtca
     3721 cacgtttacc cttcgataat ccgcttcgcc cgatggtcaa agctaggaac gcttatttag
     3781 aatcagaggg gatcaatcct ttttaccagg aatccgtacc taaagcagct ttgaagttac
     3841 gccaagcatt aggtcgcttg ataaggacgg agacagatcg tggcgtattg cttattttgg
     3901 atcgaagact agtcactgca aaatatggtg cacggatcat ccatgcatta ccgaaaaaag
     3961 taccgatcaa agagctagct atggaggaaa tctatcagga catccatgaa ttcttaaaga
     4021 aaaatgaaga aggaagttag aggagctgtc ttttttcata attaaaatct gctataatga
     4081 aaaggaatga agataaagga gcgacgaccc ttgaagaaaa aacagacaaa ggaaaaaacg
     4141 attaatcgcg tgttacttgg cttgatcgta tttttattag cggtcatcgt gatcagctcg
     4201 attttttact tgcgttccag tcgtccggcg gtgcaggcga agaaagaagc agtagaaatc
     4261 gcaaagaaat atgcaaatct tgaggaagcc gatcattttt attggtttac tcgaaagaag
     4321 acatactttt cactgacagg tacaaatgat aaaggaaaag aaatcgccgt tatcattcct
     4381 aaatctggtg aaaaagtaaa agtattggat caaacaaagg gactgactga aaacgaagca
     4441 aaacagacaa ttgctgcggc ccatcctgaa atccaaatcg aaaaagctgc tttagggatg
     4501 tatgatgatc agcctgtgtg ggaagtagta ggaaaagaaa caaatgggaa actgaattat
     4561 tacctgattg gctttgaatc tggagatgaa gtcaagacaa tcaagggaat ttaaaaggta
     4621 aaggaatgaa tgggatggag ttatcaaaaa gaacaaaacg attagaaccg tctgtcactt
     4681 tggcagctgc ggcaaaggcc aaggcattaa aagcaaaagg cagagatgtg ctgagtttga
     4741 ctgtgggaga accggatttt gcgacaccag aaaacatcca agaagcagca atcgaagcaa
     4801 tccgcaacgg gaaagctagc tattatacac caacagcagg aatcccagag cttcgacagg
     4861 caatcgttga ttatctgaag aaaaactatg gacttgtcta tgaaccttca caaacagtcg
     4921 taacagacgg agcgaaattt gctttgtata cccttttcca aacgatcctt gacccgcaag
     4981 atgaagtcat cattcctgtg ccttactggg tcagttacgg tgagcaagta aaactggcag
     5041 aaggacttcc tgtctttgtg aaaggggaag aatccaatag ctggaaagtt actgttgaac
     5101 agctagaaca agcacgttca gaaaaaacaa aagccgtaat catcaattct ccatcgaatc
     5161 cgactggaat gatttatagc aagaatgaac tgcaggcaat cggtgaatgg gcagtaaaaa
     5221 atgatatttt gatagttgct gatgatattt atggtcgttt agtttataat ggcaatgagt
     5281 ttactccaat tgcaacgatt tcagaagcaa tcaaaaatca gactatcatc atcaacggcg
     5341 tgtctaaaac atatgcgatg acaggctggc gcattggtta tgcggtggga aatcctgtga
     5401 tcatcaatgg aatgatcgct gttgcttccc agtcaaccag taatccgaca gcagtcagtc
     5461 aatatgcagc tgttgaagca ttgaaaggcg agcaagatac agtggaagaa atgcggaacg
     5521 cctttgaaga acgcctgaat acgttatatt cgcttgtggc agaactgccg ggtgtttctc
     5581 ttgaaaagcc acaaggagct ttttatcttt ttcctaatgt aaaagaaacg ctacgcatgt
     5641 gtaaatatga aaatgtcaca caatgggtag aagatctttt agaagaaaca ggggtagcgc
     5701 ttgtgactgg cgaaggcttt ggggcaccag aaaatgtccg catgagttat gctactgatc
     5761 gcgctacgtt agaagaagcg gtacgcagaa tcaagcaatt tattgagagt aaaagtcaaa
     5821 attaatccta aagaaattca aggaatataa aaggagagtc atacgtggaa caaattcaaa
     5881 tcatcgattc gaaaaaccat gtgggagaaa cagtaacaat cggcgcttgg gtcgcaaata
     5941 agcgatcaag cggaaaaatc gcatttttac aattaagaga cggaacagcc tttttccaag
     6001 gtgtagttgt aaaaagcgaa gtgtcggaag aagttttcca aacagctaaa aatctacacc
     6061 aagaaacatc cgtgttagta actggagaaa tccgtgaaga cagtcgttca aaatttggtt
     6121 atgaaatcgg aattacaaat gtggaagtgg taagcgagag ccacgattat ccgatcacac
     6181 caaaagaaca cggaacagac ttcttgatgg atcatcgtca tttatggctg cgttcttcaa
     6241 gacaacatgc gattatgcag atcagaaatg aaatcattcg tgcaacgtat gaattcttta
     6301 atcaacatca ttttgtcaaa attgatccgc cgatcttgac tggttctgca ccagaaggca
     6361 ctacggatct attcgaaaca aattactttg atcaaaaagc ttatttatct caaagtggcc
     6421 aattatacat ggaagctgct gctatggctt tcggaaaagt cttttcattc ggtccgactt
     6481 tccgtgctga aaaatcaaaa acacgacgtc atttgatcga attctggatg atcgaaccag
     6541 aaatggcttt cactcatcaa gaagaaagtt tagaagtaca ggaacaatat gtagcattct
     6601 tagtacaaaa tgttttagat cattgcgaat atgcattaga tgtactagga cgagacaaag
     6661 aagtcttaaa aacttataca aaattgccat tccctcgtat tacttatgat gatgcgatcg
     6721 aattactgaa gaaaaatgga tttgatgaca tcgaatgggg cgaagacttt ggttcacctc
     6781 atgaaacgtt cattgcgaat tcattcgaac agcctgtctt tatcctaaac tatccaaaag
     6841 ccatcaagcc attctacatg aaaccacatc caacaagaga agacgtggtg atctgtgcag
     6901 atatgatcgc accagaaggt tacggtgaaa ttattggcgg aagcgaacgg gcaacagact
     6961 atgatttctt gctagaacaa atccgtaatc acggtttgga tgaaaaagaa tattcatggt
     7021 atcttgatct acgtaaatac ggttctgttc cgcactctgg ttttggtcta ggtcttgaac
     7081 gaacagtcac ttggattgct ggtatcgacc atgtacgtga agctattcca ttcccacgtt
     7141 tgttgaaccg aatttatcca taagaggtga tgaaaaagct gttctgcatc aagtaattag
     7201 aacagccaag caaaaacagc tgttcatgtt tttgaattgg cgggacttat taccgcagat
     7261 gtaagctttt gaataccgtt tagtggagag gttgtgaaaa agctgtttag tttcgagtat
     7321 taagaaaaat tttgtggaaa tggctttcca catttttgca taaatttggc ttaataccga
     7381 gaaactggct tttgaacacc gtttaaggaa gaggttgtgc gcctgccgtt cagctccgag
     7441 tcacaaggag taatttcaaa aaacagtttt tcatgtttga tgactattgt gacttgtgac
     7501 cgaggagttg gccggagagc accgtttatt ctaaaaaaga actgcgaact ctcgcagttc
     7561 tttttttaaa atacgggata aaggaagttt ccagcagaca agagaagaaa gcaaaagaaa
     7621 ttgctatttg attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga
     7681 aggtaattca aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc
     7741 tagcacctga aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc
     7801 atgcaataat ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta
     7861 tgaccaatct atcccaagca atctagaaga gatattagta aagggacatt tactagaagt
     7921 cttagatgag cctataaata tatgttctga tggcgtcatc agtggtctga ttgacggaag
     7981 cttaggtgtg atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg
     8041 ttaagaaaaa aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa
     8101 gggactaggc tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta
     8161 tgggataata taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa
     8221 aaaacgtata ttatttatcg gtgatagcat tacagacgca ggaagagacc gagcagaagc
     8281 aactgatcta ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc
     8341 aacgatggaa tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca
     8401 agtacgctgg caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg
     8461 aatcaatgat acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc
     8521 tcagcaattt gaatccgttt atcgaaaatt actaaatgaa cttcaaacag tgggaatctg
     8581 caacatcatc ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg
     8641 taaagactta gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt
     8701 gctggttcct ttagatggta tattcaatgc tttagggatt gtttatggtt tttcgaaata
     8761 tacaagagaa gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg
     8821 gatagatgca attgccgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag
     8881 tgagaaatga tggaaatacg gtgggcagta aaagaagatc tacctcaaat caatcagctg
     8941 attactgatc atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag
     9001 aataaactat tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg
     9061 gacgtgcaac agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac
     9121 acattagtaa caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atatttgata
     9181 ctggaagcta aaaaaagaag aataaaatca ttttttgccc agttttaatc aaaaacagta
     9241 atagaaggag aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac
     9301 taatcggtgg tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta
     9361 tcccaatcgg tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag
     9421 aagtaattta tcaccagtct ggcatgtcat tgattgttaa tatcatctgg ctgatcgttt
     9481 caggcctacc aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca
     9541 tagggatacc ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg
     9601 gtgcagaaat tgtataaaag agaataataa aggttagata aagcatagag tcaggtggga
     9661 tacatgtatt tcacctgact ctatgcgttc tcctgtataa gttcttccat gtgactttta
     9721 cgtcttttat ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa
     9781 ctctttctat atttttgaaa gcgtagtgga gcttcgaata tctgggaagg acattttgca
     9841 gggattgaaa aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca
     9901 acaggcttca aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata
     9961 agagcagtgg caatgatcga aaagaaaaag aaagaataag ccaaaacatc tgtatggaag
    10021 aaattttccg atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac
    10081 cgttcattta taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa
    10141 atgaaaagtt acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa
    10201 ggagttcaga agatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta
    10261 acgattgacg cagaggaaga tcatttggct acgattgaac aagaaataaa aaaagtagtg
    10321 aaggaattag aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat
    10381 ggaaatgaaa aagagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct
    10441 ttattgctat tatttgattt taatgaacca attcgcttga tagggtattt attgatttat
    10501 ctgctgatag gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc
    10561 tttgatgaga attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat
    10621 ccagaagccg tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca
    10681 gtcagtcatt caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac
    10741 gtgcagacga cggaaggatt gatgaccgtg aaccctgaag acgtgcagat cggtcagttt
    10801 gtacttgtga agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca
    10861 ttagtcgata cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa
    10921 acagtattaa gtggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct
    10981 tatgaaaatt ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa
    11041 gcaccagctg aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga
    11101 ttggccgttt tattagctgt tttacctcca cttgttgttt ctggagcggc gttttctgaa
    11161 tggatctatc gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatctcc
    11221 gtacccctta gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa
    11281 ggttcaaatt acttggagtt gcttgcacaa actgagacag tggtattcga taaaaccggc
    11341 acattaacga aaggtgactt ttcgatccag acgatcgata caaaaatcga tccaaagata
    11401 ttcatgcaat atgttgcgag tgcagaacag ttttctacac atccgattgc tcaatctgtt
    11461 ttagaaggct acgcaggtcc gcttttacca acagctaata tccaagaatt tgcaggtgaa
    11521 gggatcttag ctgaagttga tggcaaacaa gtattagcag gtaatcacaa attgatggaa
    11581 agatttgaaa tctcttttcc ttcaagtcaa gaaatcggta cattgcttta tctagcgatc
    11641 gatcaaagtt acagtggtta tcttgttatt gcagatacct tgaaagaaga tgccgtagac
    11701 gcattggttc aattaaaaaa ggctggtgtg aagaacacag tcatgttgac gggcgattcg
    11761 aaaaaaattg ctgatcatat cgggaagcaa gtaggcgtgg acaagatata tagcgaattg
    11821 cttccagaag ataaagtgca gcggttagag gaaatcttac aaagaaacaa taaaaagacc
    11881 gcatttgtcg gagatggaat caacgatgca ccggtgctag caagagcaga tgtaggaatc
    11941 gcaatgggtg gattgggaag cgatgctgca attgaagcag cagatgtagt catcatgaat
    12001 gatcaaccat cgaagattgc agaagcaatc catttagcaa agaaaacatt gaaaatcgtc
    12061 aaacagaaca ttgtctttgc tattgggata aaaattttgg tacttgcctt aggtgctttt
    12121 ggttttgcat caatgggaga tgcagtattt gcagatgttg gggtgacagt attagctgtt
    12181 ttgaatgcca tgcgaagctt gcatgtaaaa aataaacgtt gatcaatgaa gaggacgaat
    12241 gaacgtcctc ttttttttat atccttttct taatgaaaaa tgtaaagaaa atagatagga
    12301 ttataaaata actaatgttt ttaattttgc agaaaattca aaatagcttg aaattcaata
    12361 aggtaaatga aataaaagaa aataacttat ttttttgatg gaaaaaatat gaaaaaacga
    12421 tgtgtattaa ataaaagaat acacacataa aataacactg tcttgtaagt ttgttaaaaa
    12481 ttgaatatga ttacatctgg ataacaaatt tttatcataa aggagagaac gaatgaacaa
    12541 caaacaatta cgctggttta ctgttgcgtt aattgcattt aacatggttt ggggcatggg
    12601 gaatgtagtt aataactatg ctcagcaagg agtatcagtt gtcacctctt ggctattgat
    12661 tttggccatt tattttattc catacgcatt gatcgttgga caattagggt cagccttcaa
    12721 ggatagtaaa ggaggcgtca gttcatgggt cgaaaatacg acatctaata aacgactggc
    12781 atactatgcc gcttggactt actgggtcgt ccatattcct tatttggcac aaaagcctca
    12841 agcaatcctg attgctggcg gttgggcagt ccaaggaaat ggaaatatcg taaatacgat
    12901 gtcagtacaa atggtagcag ttatttcact tattatattt cttgcctttt tatatttatc
    12961 aacaaaagga ctttctactt tgaaagtgat tggcggactg gccggaacag cgatgttcgt
    13021 catgtcgatc ttatttattc ttttagctgt aacggcaccg ttcatcaatc caacgatgga
    13081 atttgccaca ccacatatga atcaagtgaa aacatatatt ccaacatttg atttcagtta
    13141 tttcacaacc gtttcgatgc tagtatttgc agttggagga gcggaaaaaa tatcccctta
    13201 tgtcaactct acaagaaatc ctgcaaagga atttcctaaa gggatgatct tcttagcagc
    13261 aatggtaggg atttgtgctg tattaggctc agtcgcaatg ggtatgctgt ttgctagcga
    13321 taatattcct aaagacttga tggcaaatgg ggcatacagt gcttttcaaa ttttaggtaa
    13381 ttactatggt atcggtaact cattgatgat catttacgca ttgacaaaca tgattggaca
    13441 aatctccgca ttagcctttt caattgatgc accactaaaa atcttgttag cagatgcgga
    13501 tccagaattt gtaccaaact ggctacgtaa acgtaatcgt aaaggtacat tggtgaatgg
    13561 ttatcttctg acaggtatac tagtaggaat cattattttg ttgccgatct ttggtatcaa
    13621 agatatgaat gaattagtaa aatggttgac gaacttgaac tcagttgtca tgccaatgcg
    13681 ttacctatgg gtgttcttgg catatatgat gttgaataaa gcttataaga aattcaattc
    13741 tgaatataaa tttgttaaaa atccaaaaat cggttttatc tttggtgctt ggtgctttgc
    13801 cttcactgcg tttgcatgta tcctaggaat ggtgccaaaa gtagattata ctgctgatcc
    13861 taaagcatgg tggttccaat tattcacgaa tatcatcaca ccaattgtct tgatcttatt
    13921 aggtatgatc ttgccagcaa ttgctcgtcg agacaaaaac aaagaaatca ctgaaccaga
    13981 aacagtggtt cagccttaaa atagaaatac ttgttacaag aggttgtgac agaagtggtc
    14041 agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg tgaagtttcg
    14101 gcttgttttt cgcagaagct acttctggaa caccgtgaat cagaggttgt gaaaaagctg
    14161 tcctgcatca agtaataaga acagccaaac aaaaatagcc cctcatattt ttcgttttgc
    14221 tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg tagtgagcct
    14281 gccgtttaac tccgagtcac aaggagcaat ttcaaaaaac aacttctcat gtttgttgac
    14341 tattgtgcct tgtgaccgag gtgttggcag gagaacaccg tttaaggaag aggttgtaaa
    14401 atcagcgatt tgacctgagt attagaggaa caaacgaaaa aatagctcct catattttgc
    14461 cgttttttga gttaatgctg aaggtcagct gattgaacac catttattcg aaacaaggga
    14521 ctttgacacg acttttgtca cagtcccttg tttttttgcg gcgttataag tggtatgatc
    14581 gaaccaaatt ccaaagagaa gtgagggaga agaatgaaga tcatcgtgat tggacaaagt
    14641 gggtcgggaa aatcgaccct cgcaaggaag atcaaagaaa tcaccgactt tccgctactg
    14701 ccgctagacc ttttatggca tacaacagac tacagtattc aggcaaaaaa gtggtttttg
    14761 caagaacagc aatcgtttat ggtatctaat ccgtcatgga ttgttgaagg aaattatact
    14821 tctacattag aggaaaggat taaagaagca gataagatca tttggcttaa agtgccgcgt
    14881 tatctcgcca tctaccgagt agtttggcgt tctctaaaaa gaaagataaa caaaaaatct
    14941 cggccagata tgcctgaatc attttcagag aaactaaaca gggaatactt agaattttta
    15001 tcttttatct ggcattttga agaaaacaat gaaccgaaaa tacagcagct gatccaagta
    15061 gctaatgcaa gaaataaact gatcatcctt aaaacaagac gagagaaaca agaattttta
    15121 actaaattga aaatagagag caaaaataat tgacagtttt tgtttacaag cgtaatctgt
    15181 attcataagc taatgattac atttgtaaac gaaaggtgtg aaataaatgg aaaaagtcaa
    15241 aacgccagta aagatcagtg attcagagtg ggagatcatg cgtgttattt ggacacttgg
    15301 acagacgaca gcccaagaaa tcacgcagat cttagctgat tcgatggatt ggaaaccagc
    15361 aacgatcaaa actttgctgg gacgtttggt aaaaaaagaa gtgatctgga cagaacaaga
    15421 aggaaaaaaa ttcatttatc atccagctgt ctcggagatg gaaacagttc gcagtgcgac
    15481 ggaaaatctt ttttcccata tctgtgcaaa aaggattgga gagacgattg cggatctagt
    15541 agaagaagcc actttgactc aagaagatat caatgagatt ttgaaacaac tggaacaaaa
    15601 gcaaccagta gatacgatcg aatgtaactg tatcccaggt caatgtgaat gtaaacaaca
    15661 tcaataggag gaagaaaaaa tgaaacaaca attttcaatc gaaggtatga gctgcaatca
    15721 ttgtgcagca agagtagaag aaacagtaag cgcacttgac ggcgtgcaaa aagtgaaagt
    15781 aaatttgaaa aaagcaaacg gtacggtcaa gtttgatgaa acaaaagttc aatccgaaaa
    15841 gatttgtcaa gcaatcaatg gattgggtta taaagcagag gtgatttgat ggcaacagca
    15901 gaaaccaaaa tggatacgtt cgtcattaca ggtatgactt gtgcaaattg ttctgctagg
    15961 atcgaaaaag agctgaacgg acaacctggt gtggtcaatg ccacagtcaa cttggccact
    16021 gaaaaagcaa gtgtcaaata tgaagggacg accactgaaa aattaatcca aagcgtagaa
    16081 aacatcggtt atggtgcaat tttgtacgat gaagcccaca aacaaaaaat tgcagaagaa
    16141 aagcaagcgt atttgaaaaa aatgctattc gatcttatat tgagtacggt tttgactttg
    16201 ccgttgatgt tgtcaatgat tgctatgatg ttaggaagcc atgcggcgat tgttcacttt
    16261 tttcattttc caatcgtcca gttagtgctg tctgctcctg tccaatttta tgtcggtgct
    16321 cgtttttata aaggtgcgta ccatgcaatc aagacgaaag caccaaatat ggatgtattg
    16381 gtagcaattg gaacatcggc tgcctttgca ttgagcattt acaacggatt tttccgtgga
    16441 cacccccaag atctttattt tgaaagcagc agtatgatca tcacgctgat tttgctcggc
    16501 aaatatttgg aacatacagc aaaaacgaaa acaggtaatg caatcaaaca attgatgtct
    16561 cttcaaacaa aaaccgccca agtgataaga aacgggaaag aagaaacgtt agcgattgaa
    16621 gaagtagtcg ttggtgatca actagtcatc cgtcctggtg agcagattcc agcagatggt
    16681 cgaattattt caggaagcag tgcgatagat gaaagtatgc ttactggtga aaatcttcct
    16741 gtagaaaaga atccggatga cacgcttttt ggcgggacaa tcaatacgaa tggtctgcta
    16801 cacatggaag tcacacaagt cggcaagcag acagtattag cgcaaatcat tcaaatggtg
    16861 gaagatgctc aagggagcaa agccccaatc caaaaaatcg cagatcggat ctcagggatt
    16921 tttgttccga tcgtattagt catcgcgttt atcacgctca tagctacagg tttgatcact
    16981 ggtgattggc aactcgcatt gatccatagt gtctctgtat tagtcattgc gtgtccatgt
    17041 gcattaggtt tagcgacccc aacagctatt atggtaggaa cgggtgttgg tgcacgaaat
    17101 ggaattttaa ttaaaggcgg agaagcatta gaagcagctg ctcacttaga cagcattgtt
    17161 ttggataaaa ctggaacgat tacagaagga aagcccaaag ttacagatct agtcggttca
    17221 aaagaagtcc tatctatttt ttatacttta gaacaagctt ctgaacatcc actaggtaaa
    17281 gcaatcgtgg aatacggaaa actgcaagaa gcagcaacat acgatatgat tgattttaca
    17341 gcccacccag gtgctgggat cagcggtacg attaatggag tacgctattt tgcgggtaca
    17401 cgtaaacgat taattgaatt gaacctatca tttgatgaat accaagaaca cgcgttagaa
    17461 ttagaacagc agggaaaaac agtcatgttt ttagctgatg aaaagcaagt gatcgggtgg
    17521 attgccgtgg ctgatcaaat caaactagaa gccaaacaag caatcaagca gctccagaat
    17581 aaaggattag atgtttttat gctgacagga gacaacaaac tggcagccga aacgattggt
    17641 aaacaagttg gtattgatcc aaagcatatc tttgctgaag tacttccaga ggataaagca
    17701 gcctacgtag aaaaacttca gaaagatggc aaaaaagtcg gtatggcagg cgatggaatc
    17761 aatgatgcac cggccttagc attagctgat gtcggtatgg caatgggaag tggaacggac
    17821 attgcgatgg aaacagccga tgtgacgctg atgaatagca gtttggcatc cattgcacaa
    17881 acaatcgagt tgtctaggtt gacattgaga aaaatcaaac aaaatctttt ctgggctttt
    17941 gtctataata cgattggtat tccttttgcc gcactaggct ttttgaatcc aatcattgca
    18001 ggaggagcga tggcgtttag ttccgtcagt gtattgttga actccctgag cttaaatcga
    18061 cacatgaaaa agtaagagaa gggatggagt aaaaatgaat gggaacgata tggagaaaaa
    18121 acatgagcat aaaaaagaag tagtaaacaa agaaagtaca gaatctcatg aaaaacagat
    18181 ggagatgaaa catgaccata gtcagatgga tcattccatg cacatgggac ataaccatgg
    18241 agatatagca aacagcaaac aaatggacca ttcgatgcac atggatcacg aacacggcgg
    18301 catggatcat tccatgcaca tggggaattt caaacaaaaa ttttggctat cgcttatcct
    18361 agctatccca atcattcttt tttcaccaat gatgggaatg gaatttccat ttcaagtaac
    18421 gtttcctggt tctgattggc tcgtattgat cttagcaact atactattta tctatggcgg
    18481 tcagccattt ttaagtggag ctaagatgga attgaaacaa aaaagtcctg ctatgatgac
    18541 actgatcgcg atggggatta cagttgcata tatttatagt gtctattcgt ttatcgcaaa
    18601 tctgctcaat ccacatacac acgtcatgga tttcttctgg gaattggcta ctttgatcgt
    18661 tattatgcta ctaggtcact ggattgaaat gaatgctgtg tcgaatgcca gcaatgcttt
    18721 acaaaaacta gcagaattac tgccagaatc agtgaaacgc ttgacgaaag atggaaaaga
    18781 agagacagtt tcattgaaag aagtaaacga aggtgatcgt ttgatcgtcc ggtctggtga
    18841 taaaatgcca acagatggtg taatcttgaa aggtgaaaca attgttgacg aatcagctgt
    18901 gactggcgaa tcaagaggaa tcaaaaaaca agccaatgac aaagtgattg gcggttctat
    18961 caatggggat ggaacgatcg aaatcgaagt aactggaact ggtgaaaatg gttatctagc
    19021 gaaggttatg gaaatggtgc gaaaagcaca aggagaaaaa tccaagctag agtcattatc
    19081 tgacaaagtg gcaaaatggc tgttttatgt cgcattaatt gtgggtatcc tggcattcat
    19141 cgcctggttg ttcttgacag atcttcctaa tgcattagaa cgtatggtaa cagtctttat
    19201 catcgcctgt cctcatgctc taggtttagc tatcccatta gttgttgctc gctccacatc
    19261 aatagcagct aaaaacggat tacttttgaa aaaccgcaat gcactggagc aagccaatga
    19321 tctagatgtg atcatgctgg acaaaacagg aacactaaca gaaggaaaat ttactgtaac
    19381 gggtgtcgaa gtacttgatg atgcgttcaa taaaaatgaa atccttcaat atctaggagc
    19441 cttggaagcg aatgccaatc atccattggc tgttggcatt atgaattatt tgaaagaaca
    19501 tgaaatcaag ccttatcaag ctgaaaatct aaaaaatctt tcaggagtcg ggctagaggc
    19561 aacagtcaaa aaacaacaag tgaaaatagt taatgaaaaa gaagttgagc gtctcggatt
    19621 aaacgtagaa caagcattat tgaaacctta ccaagaacaa ggaaatacca tcagcttcct
    19681 gattttagaa aatcatctag ctgcaatcgt tgctttaggt gacgtagtaa aaacagaagc
    19741 aaaagaattt atccgaacat tgaaagaaag aaaaatcact cctgtcatgc ttactggtga
    19801 caataaaaac gctgcccaag cagtagcaga ttatttaggg atcgaagaat attatggcgg
    19861 tttattacca gatgataaag aagctgttgt ccagaaatac cttgaccaag ggaaaaaagt
    19921 aatcatggtg ggtgatggga tcaatgatgc gccaagttta gcccgtgctt caatcgggat
    19981 ggcaatcggt gcagggacag atatcgcaat cgattctgcg gatgtcgtat tgacaaacag
    20041 cgatccaaaa gatattttgc attttcttga tctagccaag caaacgcgaa agaaaatgat
    20101 ccagaacctc tggtgggggg caggttacaa tattcttgca attccgctag cagccggaat
    20161 tcttgcgcca attggtatca tattaagtcc agcagttggt gctgtcttga tgtcgttaag
    20221 tacggtagtt gtagctctaa atgctttgac attgaaaatc aaataataat gaaaaaaggg
    20281 gctctttgtc aataaggact gatgggttgt acaaaatcaa atctgggtca gaatgaacct
    20341 cattctggct cagatttttt gttatcttac tttgattaat tgattgatca aaaagattct
    20401 aattctcatg ttctcaaatg atttaaatcc gtaggatact c
//
LOCUS       DAPBAB010000045        20262 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPBAB010000045 DAPBAB010000000
VERSION     DAPBAB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.047"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            153..914
                     /locus_tag="RD444_001962"
     CDS             153..914
                     /locus_tag="RD444_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDT7549331.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1142..1336
                     /locus_tag="RD444_001963"
     CDS             1142..1336
                     /locus_tag="RD444_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549332.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            1605..1904
                     /locus_tag="RD444_001964"
     CDS             1605..1904
                     /locus_tag="RD444_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549333.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(2001..2435)
                     /locus_tag="RD444_001965"
     CDS             complement(2001..2435)
                     /locus_tag="RD444_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549334.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(2667..3377)
                     /locus_tag="RD444_001966"
     CDS             complement(2667..3377)
                     /locus_tag="RD444_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDT7549335.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(3367..4227)
                     /locus_tag="RD444_001967"
     CDS             complement(3367..4227)
                     /locus_tag="RD444_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDT7549336.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(4232..4870)
                     /locus_tag="RD444_001968"
     CDS             complement(4232..4870)
                     /locus_tag="RD444_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDT7549337.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(4885..5184)
                     /locus_tag="RD444_001969"
     CDS             complement(4885..5184)
                     /locus_tag="RD444_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549338.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(5227..6711)
                     /locus_tag="RD444_001970"
     CDS             complement(5227..6711)
                     /locus_tag="RD444_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDT7549339.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(6731..7192)
                     /locus_tag="RD444_001971"
     CDS             complement(6731..7192)
                     /locus_tag="RD444_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549340.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(7210..8277)
                     /gene="ulaG"
                     /locus_tag="RD444_001972"
     CDS             complement(7210..8277)
                     /gene="ulaG"
                     /locus_tag="RD444_001972"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDT7549341.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            8495..9265
                     /locus_tag="RD444_001973"
     CDS             8495..9265
                     /locus_tag="RD444_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7549342.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(9538..10366)
                     /locus_tag="RD444_001974"
                     /pseudo
     CDS             complement(9538..10366)
                     /locus_tag="RD444_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10933..12168
                     /locus_tag="RD444_001975"
     CDS             10933..12168
                     /locus_tag="RD444_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDT7549343.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(13167..13427)
                     /locus_tag="RD444_001976"
     CDS             complement(13167..13427)
                     /locus_tag="RD444_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDT7549344.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(13454..14755)
                     /locus_tag="RD444_001977"
     CDS             complement(13454..14755)
                     /locus_tag="RD444_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549345.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(15047..15505)
                     /locus_tag="RD444_001978"
     CDS             complement(15047..15505)
                     /locus_tag="RD444_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7549346.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(15518..16848)
                     /locus_tag="RD444_001979"
                     /pseudo
     CDS             complement(15518..16848)
                     /locus_tag="RD444_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(16859..18301)
                     /locus_tag="RD444_001980"
     CDS             complement(16859..18301)
                     /locus_tag="RD444_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7549347.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(18288..19052)
                     /locus_tag="RD444_001981"
     CDS             complement(18288..19052)
                     /locus_tag="RD444_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDT7549348.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(19156..20031)
                     /locus_tag="RD444_001982"
     CDS             complement(19156..20031)
                     /locus_tag="RD444_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7549349.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6413 a         4114 c         3152 g         6583 t
ORIGIN      
        1 tcttttttag aatcaaatgt gattaaatag aaaattttac aataatatgc acatataaat
       61 aataagatga tataatcaac aaagaaaatg tgattttttt cactatattt tatcacatga
      121 aaattcatta agaaagtagg aatcgaagta ttatgcacac accagaagaa attatggaac
      181 tcagtattca tcttggaaca aaaaaaatac ataaacctat cttagctaaa ctgattttag
      241 gttttatcgg aggagccatg atttctctag gttatttggc atacgtacga gtctctgctt
      301 ctatcccaga atctatggct agcttgacta gtttagttgg tgcctcggtt tttcctatcg
      361 gcctgctggt catccttctt ggcggtggtg aattgatcac cggaaatatg atggctgtgg
      421 cgattgcctt tatggataaa aaagtcacat tctcacagct ggttaagaat tggtcagtga
      481 ttacttttgc aaatattatt ggtgcaattt tcgttgccta tttctttgga cactttgttg
      541 gtttaactca ttcaggtgtc tatctcgacc aagtgattca tattgctgat cataaaatcg
      601 ctgtttcttg gtggcaagca ttaggctccg gaataggttg taattggttt gtcggtttag
      661 cgttatggtt atcatatgga gcaaaagatg cggccggcaa agtactaacg atttggtttc
      721 cagtcatgat ttttgtggca atcggtttcc agcatagtgt ggccaactgt tttgtgatac
      781 ctgctgccat atttgaaggt caagcgactt ggttagaatt tctctcgaat tttatccccg
      841 tttatattgg aaatatcatc ggcggagcta tttttgtttc aggtttttat tacgttagtt
      901 ataagcatca ttaagattga aggtaatgtt cattcgagca ttaccttcaa tctctttata
      961 tactatttat attttaagaa caatagcctt ttattatata tattattcaa attagttata
     1021 tcgagtgaaa aaaactttca aattgattct ttttatttat cataaagata tagatacata
     1081 atcattttca cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca
     1141 aatgtattat gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat
     1201 cagtaacgaa tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg
     1261 tgccataaaa ataaagaacg agctagaaaa agaactgact tgtggcaaag ctgttgtaga
     1321 tatcatcgaa aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac
     1381 aaattgctaa gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat
     1441 aaatgtttgt ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga
     1501 gctatatcaa gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa
     1561 attgtttatt tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg
     1621 aatttgacag tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag
     1681 caatttccca caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc
     1741 gtactcgttc ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc
     1801 gcatagcatt tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc
     1861 aaaaaattgt atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat
     1921 agattgagtg atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt
     1981 cttttgtacg ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta
     2041 tctatgacat aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa
     2101 aatttttttg ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta
     2161 gtgatcttct ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc
     2221 ggatgaatga ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg
     2281 attccgataa tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct
     2341 ctagctaacc ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca
     2401 gaaatctcat cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt
     2461 ctccctctaa taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa
     2521 gattctctcg attccttaaa atctctcttt ccttttagaa tgcatccttt ttgataaata
     2581 aaaaggttgt gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt
     2641 aattttctac ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg
     2701 cgaaaataat gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc
     2761 tcgttttctt tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc
     2821 ataggtgaat ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg
     2881 tttggatcta attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg
     2941 taagcttctc ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa
     3001 gcatcagcat gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg
     3061 gagtgcgtgg aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca
     3121 acatgtgtgg ggagatctga agatggtttt aagctatctt cttctattat atttcccgac
     3181 aaatctgtta ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta
     3241 atgacgatga ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact
     3301 aatccgtatc gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt
     3361 ttttcttcac aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc
     3421 ctgctggatt tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt
     3481 tccatcataa tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct
     3541 accaaaagga acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt
     3601 taagtggata gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc
     3661 cggccatgcg gacaaattgc ctaggtctgg atatacttgt agaaaagggg aatgaatctg
     3721 ttctttgatt tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac
     3781 ggaaagaatg atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa
     3841 gtcttctctg gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat
     3901 tactttgacg cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc
     3961 ttttttcttt tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat
     4021 cgaatggata cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc
     4081 agaccaatct aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc
     4141 gaagtccatt tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaatgc
     4201 tttttcataa atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt
     4261 tgaagttttg tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa
     4321 aggtatacac atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta
     4381 cagaaacatt aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc
     4441 aagtttcacc agataacagc gcatcacggc tttgatggta gattacctgc cggataccga
     4501 tgttatgcca atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt
     4561 cacctatttc tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca
     4621 tccagtctgc tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt
     4681 tcgtatcagc gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt
     4741 gtccttcctg taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt
     4801 tcataacatc tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg
     4861 gcctgctcat cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa
     4921 tttttcactg atttctttgg ctgacatcac atttttgata ccgataactg taacttcttt
     4981 tttctctgcc tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg
     5041 gccagctgaa ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt
     5101 tagttctttc attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt
     5161 tgcacacgat actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat
     5221 tttcttttat actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata
     5281 accctcagga tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac
     5341 ggcaattcca gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc
     5401 ccagtcgaac atcccgatat aaccaccata tgttgacatc ccaatccatg tagcgaataa
     5461 tgctgaaccg cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa
     5521 tccacctcgg ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa
     5581 accggctatg acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg
     5641 tcccaaagca ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt
     5701 agcagctacg tcaattcctg gaacagctcc tggtaaaatt gtgtttgaga ttccttggaa
     5761 agattgagtt agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac
     5821 cgcaaaattt aaagaagtcg tcataatgta gaataagaaa ctctgtcctt cttgcatgaa
     5881 ttgcgcttga atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag
     5941 catgagtaac gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt
     6001 gatatcttcg atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt
     6061 catccactca gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc
     6121 aaatccggca ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgc
     6181 ccaataacaa cctaaaatca gtcccataaa aatcaatact tgtatcctac ctagatcagg
     6241 aaagcaaaac agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt
     6301 agtgaaaacg gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac
     6361 aaatgcaatc agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa
     6421 tccagcatcc acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa
     6481 ccgttccttt agtccaacca aaatcggccg aaaattattg accaaaccac cagagccaac
     6541 agtcaaaata aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg
     6601 gcgtttcagc aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt
     6661 caagatattt gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct
     6721 cctctatcaa ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag
     6781 agatgagacg ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt
     6841 ctagatgttc ttctggatct tttgctgcaa gtgtaaaaaa caaactagcc gttttctcct
     6901 catcctcccc ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg
     6961 ttccaaaaac accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca
     7021 caatataagg accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa
     7081 taatttcctt atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga
     7141 tttctttatc tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc
     7201 ccttctttct tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc
     7261 aaatcctcga taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc
     7321 gcctacttgc cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca
     7381 aatttcagtg atctcttttg aatcagccat aaaattagac caaatatcgt agtggacagg
     7441 aatcactact tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt
     7501 gtctgttatc cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc
     7561 attaccatgt ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc
     7621 tcctgatgtt tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc
     7681 ttttaatacc tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa
     7741 agctactaca ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt
     7801 ttcttcagga attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt
     7861 tgcttcagga caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg
     7921 gatatgtgta acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg
     7981 ctgagtacgt aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc
     8041 ttttttcatc atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa
     8101 aatattcgtt ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc
     8161 aaaagttcca ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc
     8221 aggaaatgtt tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg
     8281 cttgctcccc ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc
     8341 atggaaaccc ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga
     8401 ttgtttcagt ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc
     8461 catggatcaa gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga
     8521 aaaacgtcaa aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga
     8581 attagctgaa tcccttgatg tatctattag tacgatacga agagatttaa atttattaga
     8641 agaaaaaaac gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac
     8701 tgattttgat caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa
     8761 atacattaac gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact
     8821 ggattttctt caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc
     8881 tccgcacaaa gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga
     8941 agcactagtg ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat
     9001 cattggctgt agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc
     9061 aacgatcaac gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca
     9121 tcgaaaaatc ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt
     9181 gatcactgat aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa
     9241 aaccattcaa gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa
     9301 aaaaatgaat aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg
     9361 aatcccaaga tgtgtccatc caatcgatac catcataatc tattgcttct tttataaccg
     9421 ttatatgttg cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca
     9481 cacctccttt tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta
     9541 tttcaggtcg ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt
     9601 ttttgcgtcc atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc
     9661 aatttcataa agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat
     9721 acccttctca cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc
     9781 gatatcataa tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt
     9841 aagtgtacgc tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat
     9901 tgccacgttt gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac
     9961 ttgattaaaa taaaataatg ctgcatctgg tttttttcgc ataaagatga tcaagcagtc
    10021 cttgataaaa taaaatttga tttaaaagat cagaattact tactttttcc tgatcgatcg
    10081 tttgtaaaat tttataagat tcttggtatt ttgatacaga attcaatgat tgaatctcat
    10141 ttattttttt cctgattttg tcttcttctg tttctactaa tatatcggag atctcgacat
    10201 tcagcttttt gcaaagcgca cttaagatat tgatgcctgg cgacatattc ttgttttcga
    10261 tattgctgac agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt gttaacccct
    10321 ttttctttcg gtattctttt agtaatgctc catctacttt aatcaaaaca atttccttcc
    10381 ctttaaaaaa ttatttctcc tttatatcat acacaaaaaa aagttctaaa caaccaaaaa
    10441 tgtttagaac agaataaaaa ttttaatttt aaaataatat tcaaattaaa caactggcgt
    10501 atctgctcct tgatttcttt ttgtgttatc tgaataatta tcgtttacag ttataacaca
    10561 tagtgaaaat tgtcatggcg agaaagtcta agaatatttt taaacataac tatttctttc
    10621 ttacgtgtgt ataatgacga cagcacacct atcaattaat ataattattt tattttttat
    10681 gttctcatgt ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat
    10741 cgatcaaaaa attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt
    10801 taaatttaga agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct
    10861 actcgaacgc tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat
    10921 aaggaggatt tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca
    10981 acacgaatga ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc
    11041 ttttctaaaa cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg
    11101 ccgatcctta cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt
    11161 aaaaaccctt ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt
    11221 gctaagtttg agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc
    11281 cgcaataaaa agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt
    11341 caatcagagc atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc
    11401 ctttggcact ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa
    11461 cacgctgcac aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct
    11521 gatctaaatc tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat
    11581 tatcgaatcg ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta
    11641 acttttatac agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt
    11701 tcaggcaatc cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta
    11761 gcggttattg cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt
    11821 ttaataacag aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc
    11881 cacggcgggg aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg
    11941 gggatgcaat ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc
    12001 gccctcacaa aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca
    12061 cccaagattc gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa
    12121 caagcaattc aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa
    12181 aaagcttttt taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa
    12241 tacaaaaaaa gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa
    12301 aacacaaaat catccttaaa ataaaagatc tgacgcaatc caaatgagca taggtagcgt
    12361 ctgccattta ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt
    12421 ttcttcaaaa gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata
    12481 gatctagaag catcgctttg tagagatttg tgctgctagt cccttcaaca attactaaat
    12541 caaatgtagg atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat
    12601 gatctcgcaa tccgatgcat actcctatat aaactgccag caatccccac atgctgagta
    12661 gatcatgtca gaatgttcta tgtcgtcagc aacgataaac taaaagtcac gttcaccatg
    12721 actgattgct attcccagcg caattatttc tatacacgtt tcatgatttg aataaacact
    12781 ctcatcatac atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc
    12841 tcccaaatct atatttgcct tgatcacttt ggtagtagat cgactaattc ttaatccatt
    12901 gctgataaaa ttttcatttt tgcctccttc tttctgccaa aacagttaaa acaataataa
    12961 tctttatgta aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac
    13021 ggtggtcaaa agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat
    13081 ttcacaaaaa tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac
    13141 ttctgtcaca accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc
    13201 tcgatcataa aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa
    13261 acattcatat gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc
    13321 caaatccatc gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta
    13381 gccgttaata aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt
    13441 ctaaaaacca aacttagaca gtttggactt cgttttctcc acgactttgt tcatctgaca
    13501 gcatcttttt atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt
    13561 tgatcatgat caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg
    13621 gagctggtaa tgtccaagtt ggcaaaacag taaatctatt gaccatttct aatttgactg
    13681 caaaataact gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag
    13741 gattcaacat cataggagct ccaaaaatca tgggctcatt gatattgaag ataccaggaa
    13801 taatagaaaa acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa
    13861 cagataccac gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt
    13921 caggagcgat atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct
    13981 catccaacat gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga
    14041 taccaaatga ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat
    14101 tccctaaaat attggttaaa ggctcgaata acgataataa ggtagcgttt acatcaaagc
    14161 ccgcgacgct tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct
    14221 caaaactacg ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat
    14281 ttttaaagaa acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc
    14341 cagatgctcc caaagaactc ataggtaaga cccagccaag aggtgccttt tcaggagtta
    14401 aaacgtttac aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca
    14461 cactatctaa tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag
    14521 ccatcaatcc catcgtcatc ctaaaaggta aaacaatcgt tgccgaataa ggggcaataa
    14581 attcattcca tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa
    14641 tcaatgacaa tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa
    14701 tttttgccat tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa
    14761 ccactccttt tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata
    14821 actaattata taattaagaa attttatttc aatatattct cttaaataat caaatttatt
    14881 tttaagaatc ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt
    14941 aacaaaaatc aaaatgatta aatctagtga ttgaaactta tcatttcaaa aaaattttgt
    15001 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
    15061 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
    15121 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
    15181 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
    15241 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
    15301 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
    15361 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
    15421 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
    15481 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
    15541 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
    15601 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
    15661 ctagtaactt ctctgctgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
    15721 gttttcttag tgtaattgtc tccacaccag ttgcaccaac atatgccggt atttcaacaa
    15781 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
    15841 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
    15901 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
    15961 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
    16021 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
    16081 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagttttg tctcctatat
    16141 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
    16201 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
    16261 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
    16321 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
    16381 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
    16441 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
    16501 cgagtccaca cgtttcctgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
    16561 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag
    16621 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt ttttcagcat
    16681 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
    16741 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
    16801 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
    16861 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
    16921 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
    16981 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
    17041 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
    17101 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
    17161 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
    17221 cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
    17281 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
    17341 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
    17401 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
    17461 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
    17521 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaacctgata
    17581 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
    17641 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
    17701 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgccc
    17761 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
    17821 agcccaaaaa aatagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
    17881 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
    17941 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
    18001 tgatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
    18061 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
    18121 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcatcagctg
    18181 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
    18241 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
    18301 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
    18361 aaaaattcga cttggtgagt acgaggatac acaaaacttg aaccatttaa caattcctga
    18421 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
    18481 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
    18541 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
    18601 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctggttt
    18661 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
    18721 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
    18781 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
    18841 ttgagaagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
    18901 tcctttctta gtttcacagc gtgctcgaaa cccggcatat ttgccatcaa tgtagttgat
    18961 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
    19021 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
    19081 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
    19141 agttacgccc ttattttaat agattcgtgc tagatgaatt gccaaataga tgatttcatt
    19201 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
    19261 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
    19321 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
    19381 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
    19441 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
    19501 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
    19561 gattcgctcg accacttcta ttgctatatc atattcattt ggataaagga atctcatttc
    19621 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
    19681 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
    19741 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
    19801 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
    19861 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
    19921 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
    19981 taattcacct ccactatatg ataaaacaaa acaacttgca gactccttca tacatatttt
    20041 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
    20101 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
    20161 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
    20221 gtaattacat aaaaacaaaa aaaatatagc aactctctat aa
//
LOCUS       DAPBAB010000046        20043 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPBAB010000046 DAPBAB010000000
VERSION     DAPBAB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.048"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..394
                     /locus_tag="RD444_001983"
     CDS             <1..394
                     /locus_tag="RD444_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDT7549350.1"
                     /translation="TEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTETQAAPVAETQ
                     PAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQ
                     ERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(478..1200)
                     /locus_tag="RD444_001984"
     CDS             complement(478..1200)
                     /locus_tag="RD444_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDT7549351.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQSYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKTVPIDHLLIETDGLHAAEWALGGIFSEKYYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1190..1927)
                     /locus_tag="RD444_001985"
     CDS             complement(1190..1927)
                     /locus_tag="RD444_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549352.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYRNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1939..2931)
                     /locus_tag="RD444_001986"
     CDS             complement(1939..2931)
                     /locus_tag="RD444_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7549353.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQETKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDITYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDNPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2946..3689)
                     /locus_tag="RD444_001987"
     CDS             complement(2946..3689)
                     /locus_tag="RD444_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549354.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3763..3867)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3930..5252)
                     /locus_tag="RD444_001988"
     CDS             complement(3930..5252)
                     /locus_tag="RD444_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7549355.1"
                     /translation="MKTYDAIVIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYFVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANVVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRNPPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5239..6204)
                     /locus_tag="RD444_001989"
     CDS             complement(5239..6204)
                     /locus_tag="RD444_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549356.1"
                     /translation="MTKLIEFKHVQKKYDRKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFNEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6201..7739)
                     /locus_tag="RD444_001990"
     CDS             complement(6201..7739)
                     /locus_tag="RD444_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDT7549357.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKVLNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8155..8442)
                     /locus_tag="RD444_001991"
     CDS             complement(8155..8442)
                     /locus_tag="RD444_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549358.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8576..9244
                     /locus_tag="RD444_001992"
     CDS             8576..9244
                     /locus_tag="RD444_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7549359.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9530..10270)
                     /locus_tag="RD444_001993"
     CDS             complement(9530..10270)
                     /locus_tag="RD444_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549360.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKGNLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKMLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10400..11347)
                     /locus_tag="RD444_001994"
     CDS             complement(10400..11347)
                     /locus_tag="RD444_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7549361.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11476..12240)
                     /locus_tag="RD444_001995"
     CDS             complement(11476..12240)
                     /locus_tag="RD444_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7549362.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRGSEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12244..12633)
                     /locus_tag="RD444_001996"
     CDS             complement(12244..12633)
                     /locus_tag="RD444_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549363.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12767..13309
                     /locus_tag="RD444_001997"
     CDS             12767..13309
                     /locus_tag="RD444_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDT7549364.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13457..14089
                     /locus_tag="RD444_001998"
     CDS             13457..14089
                     /locus_tag="RD444_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549365.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14223..14348)
                     /locus_tag="RD444_001999"
     CDS             complement(14223..14348)
                     /locus_tag="RD444_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549366.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYSILWEELAA"
     gene            complement(14482..15378)
                     /locus_tag="RD444_002000"
     CDS             complement(14482..15378)
                     /locus_tag="RD444_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDT7549367.1"
                     /translation="MNYYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKK
                     EFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLS
                     VDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRATAGNEKYRFITLSSIEHF
                     PLTLANYFFVQKNQLPVTKAFKELAELIGDVNEAYFFDQIEVIVDRIRKNKYKNRRPS
                     IFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEETAFLKRQIFSELS
                     KKFIPHAFSYIKEYRTVLVSKT"
     gene            complement(15560..16486)
                     /locus_tag="RD444_002001"
     CDS             complement(15560..16486)
                     /locus_tag="RD444_002001"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDT7549368.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16571..17332)
                     /gene="pflA"
                     /locus_tag="RD444_002002"
     CDS             complement(16571..17332)
                     /gene="pflA"
                     /locus_tag="RD444_002002"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDT7549369.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGNKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17576..19807)
                     /gene="pflB"
                     /locus_tag="RD444_002003"
     CDS             complement(17576..19807)
                     /gene="pflB"
                     /locus_tag="RD444_002003"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDT7549370.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
BASE COUNT         5738 a         4097 c         3392 g         6816 t
ORIGIN      
        1 aacagaaggc tctgcacaag cagctgctga acctcaagca gccgttcaag aagcaccagt
       61 ccaagctgaa caacctgttg tacaagaaac ggttcaaacg gaaacacaag cagcgcctgt
      121 tgctgaaaca caacctgctc ctgctgtaac agaaactgct gctactccag caagtacaag
      181 ctctgcaaaa gaatggatcg cacaaaaaga atcaagcggt tcttacacag caacgaacgg
      241 acgttacatc ggccgttacc aattagattc ttcttactta aatggtgact actctgctgc
      301 aaaccaagaa agagtagcag aacaatatgt tacttcacgt tatggttctt gggaagcggc
      361 gaaagcattc tgggaagcaa acggttggta ctaagattca tttattaatc gaacggttta
      421 cttttataca aagaaagcca aaaagaggtg tgacacaact agtgtcacac ctccttttta
      481 tttacctaat ctaatgaaac gttccaagtt ttgataagta tttttcttaa cttcttcaac
      541 tgatagctct ttgatctcag cgattgtctt taactgttct tcaagcagtg agacataata
      601 tttttctgaa aaaatccccc ctaaagccca ctctgctgca tgtagtccat ccgtttcaat
      661 aagtaagtgg tcgattggga ctgttttagc tagttgccag actgctgggt tctttttcaa
      721 atcaagacca atgctgaaat aacagcctaa atcaatatag tcttgttgat agtccaaaga
      781 agaataccaa tgaatcaaat agtgattagg gtaagattgg atataattta agatctcttt
      841 ttcgcatcct tttgtatgca gtacaacagg tttctcattt atggctgcaa aagcaagctg
      901 tcgctcaaat actttttttt gtgtagtcat cggtacattc gtccaaatat tatctaaccc
      961 tatttcaccg atgatccttg cttttttcaa aaaaggctca acttgttcaa acgtatagct
     1021 atctgctttc caaggatgga taccgtaact taaggcttga gtcttacttg ctgcaagctg
     1081 tctgttttcc ttccattctt ctggcgaatc acaattgatg atcgtcaaaa tcgtgtgttc
     1141 ctgctgaagg agtgctaaac tctgactccc atctaaatga caatgagcat cacttaacat
     1201 ctaattcacc tgccaataac tgataaatcg tttgcttcaa ctctgattgc tgcagtagcc
     1261 attcacgtga ccgtcgtttt tcaggcactt gaatctcagc aatcacatgt gccggacggg
     1321 cggaaagcac aagaatcctg tctgataaat aaattgcttc ttcgatatca tgcgtcacaa
     1381 gtaaagtagt ccgctttgct tttttaggca gcttcaacat ccagtcttgc atctgccctc
     1441 tggtcatagc atctaacgct ccaaatggtt catcaagaag caagtaagaa gaaggattca
     1501 tgattgttcg taacaaggcc gttcgctgct tcatcccgcc agataattgt tccggcaaaa
     1561 aatgttcata tccgtttaac ccaaagatat ccagataatc ccttacttgt tcttcattga
     1621 ttgttagatg attgattttt tgatataacg taacgtttcg atagacgctc agccaaggaa
     1681 gtaacatatc ttcttgcggc atataactaa atgctgtatg gagaccagtt actttttgat
     1741 cgtctacttt gatcgttcca gaagtcatag gctggactcc tgttaaagtt ttcagcaatg
     1801 ttgacttacc ggctccgctt ggcgcgacaa tcgatgtaaa ggtatcttta gggatagtga
     1861 aatggatgtc ttctaatatt ttcaggtttt caatgactac attcaagtgg ctgatttgga
     1921 ttgacatatc tatcactcct attttataaa ctgattagtg aatagttctt gcgaaggtac
     1981 atcttgcttg atcaatccgt tttcctgcat aaaagctgta taattgcgcc aaactttttc
     2041 ttccatcaat ccccaattct caggattatc cgtataattt tttgacaaga atgcttgtga
     2101 ttcttctaaa aacgtccgat cattttcttt tgcatatttt tgcaacagtt tagcactgtc
     2161 atttggatcc ttaatagctt cctgatagcc ttttttcgtg gcatccataa aagactgtac
     2221 caattctgga tcacttttga tcagttgatc attcgtaata attaccggag tatagtaatc
     2281 taaccgttca tctaattctt tcaatgggat atagtttagt tcaatacccg cttcttttgc
     2341 tttgatcata tcccaacctt caaagtacca ttggatatct gaggattttc ctaagctttc
     2401 tggcccttct ccattgcttc ctaccattgt cagttttgaa aagtccccgc cgtctttttc
     2461 catcacagct tttaagacag cttcttcact tggactttgc caacctgcat atgtttttcc
     2521 ttcaaaatct tttggtgaat ggatgttact gtctgatagg gtagcgaatc cagaagtatt
     2581 atgtttgatc actgtagcaa ttgctttgac tggtatattc tgtccatctg cgtgagcata
     2641 agtgatatct tcttgataac ttacaccaaa ttgtgctttg tccgcaccta ctagaccaat
     2701 acttgtcgaa ttatctcctg gttcgatgat ttggacattt aaaccagcct ctttataata
     2761 gcctttttct tttgctaggt agatgccagt atgattagta ttcggtacat aatccaaaac
     2821 aagtgtcact tttttcaaat cctttgtttc ttgtttgcta cttgtttctt ttgcagtgcc
     2881 acaagcggat aaaagcaaca gtagcggtaa taaaagccat aattttttca tttgatcccc
     2941 ttctcttaaa aaatataaag catacgataa tacccttttt tcaataatga acaagcgcca
     3001 ttcagcaata aactcaatag aatgacacaa atgatcgcag caaaaacttt atcgatctgg
     3061 tagctgtttt ttacgcgtat catgtaatac cctaagcctg cttgagcact tagccattct
     3121 ccaacgactg ctccgctgac acaataagta gcagccactt ttaaaccgga aaagaagccg
     3181 gataaacccg ctggtatttt atatattcga taaatcttcc ctgtatctgc ccccatcgtt
     3241 ttcaaaaaga tgatttgctc ttttgatact ttcctcaatg tatccgaaaa agaaactacg
     3301 atcggaaaat agcttatcaa gacaacaagg atcacttttg gaagcatacc gaaaccaaac
     3361 catagggtaa gtaatggccc taacaccatg attggcaaca tttgtgaaat cactagtaac
     3421 ggataaaaag acatttctaa aaacttccaa taatccattg ccaaggcagt aataaaagaa
     3481 agaacgctag ctattaccaa accaagcact gcttcattta atgtcgtgac agaatgggaa
     3541 agtaatactt gtgcatctgc cgctaatgca gaaaatacag cagcaggtcc gggtaaaata
     3601 aaggcaggta ttttccaaac tatgacaaca atctgccaaa taattaaaat gaatgccata
     3661 ctgactgctg gataaagatt tttcttcatt ttgttacctc ctttcttcta atatgaataa
     3721 caaaaaacgc ttgaaatggg cacgacatag taacccgctt caagcgtctg ctatgtttct
     3781 cccttcacca gtattaactg acaggttcaa tgggtctaag ttcacaactt actctcatgt
     3841 cgtctacgac atccccagaa acaggtattc attttttaaa gtgtagcaca agaaggaaaa
     3901 agcaacaagc gctttagtta tcatgctgat tatttcaata aataggagag atcacttgcc
     3961 aatgtcggat accccatgat atagctatcc aactcttttt tagtcaattt ttgattgatg
     4021 actagtgtca aatcatcgat caattcgtct gcttgctcgc taatcactgc agcaccaatc
     4081 aaataatttt gctggtcaaa ggtcaattca gccttagcaa ctggttcatt gacacgatga
     4141 taggtgaacc aactggtaag atccatctct accacttgat ctgacgaaga agcgtgactt
     4201 tttgtaacac ctacttcagc aagtttagga ctggcgtaga cgatcgtagg tataatcgga
     4261 tatttgatag gttcgcttgt tgcatccgtt atccgcttag ctacataatt tccttcaaaa
     4321 gtggcgactg gtgtcaattt gggggggttc cttgaaacaa tatctccaca cgcaaaaatt
     4381 tttggattgc tcgtttgcag ataatcattg actgcaattc cgtgtttatc aaagacgaca
     4441 ttcgcttgct caagtgctaa agattctgta ttcggctgtc tcccagtcgc acaaaaaatc
     4501 atatctgcta ctaattccgt ctcttttccg actaaacgat agcgagtacc ttctgaaatg
     4561 atcttttggg tttcaacacc aaaagcaaac tgaatgccag atgcttccat ttgatggacg
     4621 gcttcctcta ccaaagacgc ctcaaattct tttaatggcc gttgattatg atggacaatg
     4681 gttactttgc tccctgcagc attggcaatc gtagctagct caaaagcaat ataaccacca
     4741 ccgataaaaa tgatttcttt aggcagtaca ggcaatgata aaaagtctgc actagttttc
     4801 aagtattcct ttccctccac tggtaagatc gtaggtcttt gcccggttgc caaaacaaag
     4861 taatccgcat ggaagacttc ttcgttgact tcaatagatg aatcatccaa aaaacgagcg
     4921 gttcctgaaa gatgatcgat ttcagcttct gctaattgtt tttttcggct ttctgggact
     4981 ggatccgtaa atgttcgttt gaatttttga agttcttccc agttggcagt tgggatctcg
     5041 ttaaaacctt ttccactcaa ttgttttacc cgattacgtg cctctactgc acttaacaag
     5101 acttttttag gatcacatcc acggttagga caagtcccgc cccatagatc ttcttcgacc
     5161 actagtactg ttttacctgc ttctttcaat ccataagcag cacttaagcc gctcacacct
     5221 gaaccgatga cgattgcatc atatgttttc atttccttcc tcctctcgac ttaaaaaagc
     5281 aaagatttct ttacttgtca gcgacccaat acttctttgc ttttctacta tcacaatgct
     5341 tggatacacc gaaagaagcg aatatacttc tttcataggc gtatcaaagc aaacttgttg
     5401 acgagcattt tctggtattt catttaagaa atatccattg acgactaaat cttctactcg
     5461 ccactctttc gtctgtttcg gttcattaaa gaaatgccga acgtagtcgt ttttaggatt
     5521 catcaataac tgctctggtg tgtcacattg gataatttct ccatccttca taaatgcaat
     5581 acgatcacct aacttgattg cttcttccat attgtgcgta acgaaaacga tggtcgtgcc
     5641 caattcttcg tgtaaggaca aaaccaattc ttgtaatgag tttcttgaaa tcggatctaa
     5701 tgcgctaaat ggctcgtcca tcaaaatcac atctgggctt gcagcaatag ctcggatgat
     5761 cccgatccgt tgctgttctc ctccagacaa ctctctaggc atacgatctc gataaatatc
     5821 aggagataag ccaacttttt gaagaagttc atctacacgg tctgctcttt tctgcttttc
     5881 ccaacctaac atttcaggga tgacttcaat attttgcttg actgtcatgg tcgggaacaa
     5941 agcgatttgc tgcagcacat atcccatatt ccaccgcatt ttctgcagat tatattcatt
     6001 taacgatttt cctttgaagt agatgtctcc tgctgatggt ttagataatc cattgatcat
     6061 tttcaaagta gttgtttttc cactaccgga aggaccaacc aaaacaaaga tttctccttt
     6121 tttgattgcc agatttaggt catcgatgac atactttcta tcatactttt tttgcacatg
     6181 tttgaactcg attaatttag tcactgatct tcctccttta ctaatccttc atttttcagg
     6241 aagattcgtg ctacttctgt cggctttttc ttttcgacat tgacttggta attcatatca
     6301 atcatttgat cctctgttat ttttccgctt aatttattca gtactttaac gatttcagga
     6361 tgttctttag caaaatcttt tttcatcagc ggtgcgcctt gataagctgg gaataaccct
     6421 cgatcatctt ctagagtcac tagatcgtac tccttgagct ggctatctgt ggaataggca
     6481 tccacaatat tgacatctcc attattgatt gcttggtaac gcaaacttgg ctccatgctt
     6541 tgtacacttg aaaaattcaa gccatatgct tcttggattc ctttatatcc atctgaacga
     6601 tcaataaatt ccagtgtgaa tcccgctttg atctgatcag agatagctgc caaatcggaa
     6661 atctttttaa gttgatgttc gttcgcaaaa gatctcttga ctgccaaggc atacgtattt
     6721 tggtaagcca ttggttttaa taatgtcata tcgaattgct tattcaataa ttcttctgct
     6781 tcgtcatatg ttttttcttt tgtcaaatca gcacttttca ttgcttcagg taccttcacc
     6841 aagctttcta ggacagtacc tgtgaactca ggatagatat cgatttgacc ttgatccagt
     6901 gcattgaaca aaaaactcgt cttgccaaaa ttcggtttta attcaacttc tgtttttgtg
     6961 ttgtcttcaa tcaattcttt atacatttgg atcaggattt caggttccgc tcctaatttt
     7021 ccagcaatcg tgatggtttc atttttaaac aaggcagact gatataaaga aacgccgcca
     7081 aataaacaca ccactgctgc taaagtaatg atggaatatc gaggtttctt atgctgcaac
     7141 cagcgaatca gtccgctgaa tataattgca agcaaggcag aactgatcgc accaatcaat
     7201 gtcaaaacag gtgtatttcg gtcaatacct aacaaaataa atgtccctaa tccacctgct
     7261 ccgatcaatg ctgccaaagt agccgtaccg atgattaaaa ctaaggcagt tcggatacct
     7321 gagataatga caggtaatgc gatgggaagc tctactttca gcaagcgtct cattctggac
     7381 ataccaaatg ctaccgctgc ttcttcaata gacgggtcaa tttcagctag accaatatac
     7441 gtgttttgga aaatcggaag caaagcgtaa ataacaagtg caatcaatgc tggaacagtt
     7501 ccgatcccta caaatgggat aagtaaacct aataaggcaa gtgaaggaat cgtttggagc
     7561 acacccgtga tctgcaataa gacttctgcc atttttttat gattggctgc ccaaatcgct
     7621 aaaggaatag caatcagtgc tgcaatcagc aaagaaatga gagaaatgga cagatgttga
     7681 aatgcagctt ccagcaactc gccttttctt tcacttaatg tttgtataaa attattcatg
     7741 cgaccttcct ttctgttatt ctttaagtat aaacatttct ttcctttttc ccaactaact
     7801 tgtcttgcct taaccttaca gcacgaacaa aaaaaaagaa aaaaatacct ctaaatatgc
     7861 tgacaaaaaa gaatccaagc aacaaagtcg gatcaacttt ttggttctcg ttttttcact
     7921 acgaatacca taaaaatcta tacgaaattg tcacttggaa tcagctatat cttttttaca
     7981 cacacaactt ttattttaat aaaaagaaac agccaataaa gtagaaatct cgtgggggag
     8041 aacaccttta ttggcctgtt caacgaatat tatactgtat catgacactt ttttgccaaa
     8101 aatgtaaaaa acggttgttt tgacatcata aacgtcttgt tttcttattt ttacttatat
     8161 caccacttta ttttctatct ctttcactcc ataaagcgga atgagttctt cttttgactg
     8221 ataataaaca gctttctcac ggttcttcat caaaaaaaga aagatgtctt tcatagcaag
     8281 tggtgctttg ccgtgttcaa ataaaaagat aaattgattt gctttattta tctggaccat
     8341 tgtcacaggc accagttttt gttcttgagc caaaaaaact ccttgatact ctgttttttt
     8401 gggtgatttt gtaagaattg tcaataagtc tgctgctcgc atttttccac tccttatttg
     8461 ataaataaaa agaaacagcc aaaaataatt ttttttcatt ttcctcttga agtaagagta
     8521 taagtcagta taataaagaa gttgctgaaa attttcaaaa gaaaagaggc tagaaatgaa
     8581 aaaattaaat ttatttacag aattgtgcgg aatcatttta ggcgcaggaa tctacggatt
     8641 agctgtgaca ggaatcaata ttccatctaa gctggcagat ggtggggtta ctggtattgc
     8701 tttgctcttg cataatcttt ttggattcgc tccttctgtc acttcactgc tttttaattt
     8761 acctttattg cttatctcat tgtttctttt tggaaaacac gcttttctcc ggacgatcgt
     8821 cggaacattt gcgctagttt tctttttaca tttatgggag agccttcata tacattttgc
     8881 tgtagatagt ctactgatca atagtttgat gacaggtatt ctttctggca ttggttgcgg
     8941 tctcgtcttt cgattcggcg gaagcacagg cggaacagat atcatttatc aagccattga
     9001 aaaatatttt catattaaga ttggcaaaag tcttttcttg attacttttg gtattttagt
     9061 tgtctccttg ctttatttgg atctcacaca ttttctgtat acattactta gctgttctat
     9121 tctatcttat acattgaata aagtgaaata tctggaaatt ggatctgttt tgccccaatt
     9181 ggaagcaaaa cagactacag aaacatttga accacttgac cctcttgaac agttagagca
     9241 ctgattctta tttttcacta ctttatttac agaaatatca ttatgtaaac taaaatgata
     9301 ttattcagta aaatcttctt tataaaaaaa taaactattt ttgcaaacga tctgcgaaaa
     9361 tagtttgttt ttttattttg ccattatttt cttacttatt gggagaaaaa attcgcttaa
     9421 gctcaaaaaa atctgaggat acgatataaa cttccggata gagtataaaa taaggaaacg
     9481 ccgcagcgtt tcctttccaa ctgtagccga atataaattt gtatatcact tatttaaaat
     9541 agctattttc tcccaaagag tttccttgat catcgatcaa tccttgtaat gagcgttcta
     9601 aaataccatg gctttttttc gatttttttt cttctgactg ctttgtttcc tctaacattt
     9661 ttgttcgatt gaatgttcgt acagacggct caccatttct tttttcttga tacggcgtcg
     9721 gttcagtatc gaacaacaga taagagtcat ccgttttttt catcgattcg attaaatgat
     9781 cctcagaaat tttgctttct ctagggtctg ggataatcga tgcgggtaca tatttaggaa
     9841 caaaataaga acgctcccgc accttcgtgt caacagatgc gtttcgtgta gtatgcttgg
     9901 actctgtcct tgtttcactt tgtctgacag aacgagagga atcaccaaaa agtttttttc
     9961 gtcctgtcga tgtgatttct gattggtaat ggccggtata atccggtaat tttcctttat
    10021 gcttttccaa atttccctta cgcggagctg acttcaatcc atttgttgca cgacgcttgc
    10081 gacggatttt ctcggtgttc gtgttcgtga aatgaaattc tttcatctgc tcaaacgggg
    10141 actgattctg ttcagtcaaa agatcttctt gtccgtcaaa taatactcgc ttcttgtcgt
    10201 tggttaattt tacgcctaac tcatcatcgt acgcaggaaa acggaaatgt tttcgtctga
    10261 tttctttcat tcaaaccatc cttctcaaaa atggaacttt tctcaataat agcacagatt
    10321 cattgttttc agtagcaatg cgctgattat tttaagaaaa tagcgatgaa tttcataaat
    10381 atatatatag taataagact taattcgttg tctctctgat aactagttca ggaaaaaaac
    10441 gacgataacc ttgactcgcc cctttttctt ctattttttt taacaacatt tctccacata
    10501 cttcacccat acgtataata tcttgcttga tcgttgtcaa tttaggtgaa ctgatttggt
    10561 ctaaaaatac cccatcaaat ccaatcacac caaattcttc gggcaagtgt cctccagcat
    10621 ttataatccc tcttactgct cctagtgcta gccgatctga actacataca aaacaagttt
    10681 tatctgtaaa atctgcccag ttttctgtca tatatctttc tacttctgtc gaatgatttg
    10741 gaaaccgcaa gattttacta ggaagatcaa cttccttcat cacagataaa taaccagttt
    10801 cccttgaaat ttcaaaaggc tcgtccactt cgataccgat ataaataatc gtttgataat
    10861 ctctttctaa ggcatattgc gttgcttttg ctgtaccata ttcattatcg ctgtctacat
    10921 aatcatatcc ttcagaattt tccccaaaca agacaactgg tttttccaaa cgatcgatcc
    10981 aatcgaagtc tttttttctg acacctgtga tgatgtatcc atcacaagaa ccgacgtcga
    11041 aggcatcatt tgtaactaat tggagagaat aatgcgcttt accgatccgt ttagcgattc
    11101 ccatcagcaa attcatgtaa tagggctcag ttgtgtcgat ttcttccagt ataaataatt
    11161 tgatgatctg cgaccgattc gtgacaagtg cttttgcagc aatatttgga cggtaatcca
    11221 gttcagccat tgctgagaaa actaactctt tcaattcgtc agtcacctgt tccggatgat
    11281 tgatgactcg agagaccgtc atttttgaaa cattggcacg ttttgcaaca tctgctaaag
    11341 tgggcatgct ctcttccttt ctaacatgtt gtcattctta ctatcaatta taccaaattt
    11401 tcctaacaga gtttattata gcccaaaata aaaaacggaa aatcctctat cgtcatgagt
    11461 gattttccgt ttcaattatt tatacttttt tatcaaattt tttgctaaaa aagtggaagc
    11521 ttcttccatt agtggtattt gtaccatatg tcgctgtgtt tttgaggaac gaaaaacaat
    11581 ttcttttccg taagacttat gccgattttt ttctacaaat cgttccgttt gtatataagg
    11641 gattttttcg tcagcttgtc catgccaaaa gaacagagga cgtccagcta acttttccgg
    11701 ctgcaatgac aaatcatagc tcttaaccca tgaaatcaaa tcaaaatagt cttttggtac
    11761 gacataatta tattccgatc cgcgccttac gatttctttt ccatattcca aaggtgcagg
    11821 ggaacccatc aaacacacgc ccgctactat atgaggatgt ttagccaaca aggcgcatgt
    11881 tgtcattccc cccatcgaaa ccccgccaac tgcaagctga tcatgtagaa gacctctttt
    11941 ttctaaatgc ttgataagtg tgtcaaattc aaacagattt ccgtagatac tatcccaaaa
    12001 tgtataggat ggaatagaag aaaccggctg tttgcgctcc ccatgattca tcgcatctgg
    12061 caggatcaca cgaatgccct gctgcgcaat ttttcttcct tgcgtcaaaa ctaactcctt
    12121 acttgtctgc catccgtgat aatagatgac aacgggaata cgttcctctc ttaattcttt
    12181 cagaacaact tctaacactg gaatatgatg gacagaacgt ttcaatataa ttggattcaa
    12241 ttgttactca cctattttca ttaaatttcc tgatcgccat agcaaccatg ctccaacagt
    12301 tgtctggaac aggttcagtg taccaaataa tataaatgca ggactgaatg gctcgggaaa
    12361 taagcacaga gaacaaagtc ccaccactcc taatgctagc gaaaggagtg gataaataga
    12421 aaaattcaaa aaactggcaa cgctaccgaa aatattacca ataaacagta tcaaaaagaa
    12481 aaaaacgctt aaaattaaac catctgtcag cgacatctga aaaacaatgt tctctacctc
    12541 aattggctgt tgaatcgaat agacacctaa gtatatactg agaaggagac ttagcacgtg
    12601 gctggcacaa aacacacgat tgaccgtttt cattttaatc acctaaccta tctttataat
    12661 tctattataa tcattttatc attttttgta tgtataaagt ttcaattttt cttcacttac
    12721 gatttttttt gatattgttg ttatataaat gaaaggagtt ttacaaatga ttgatccaaa
    12781 aatgttgggg atgatcttcc tcattaattt tgcttatatc actctgaaca cgttacgttt
    12841 catgttgaca atgaaaggct atcgactgat tgctccactt gtcagtatgg tcgagatcac
    12901 gatttatatc gtcggcttat cccttgtttt agatcgttta gataatccac tgaatttatt
    12961 tgtctatgcg ttaggttacg cagtaggtat cagtgtcgga attttgattg aagacaaatt
    13021 ggcattagga tacatcatgg tcacaacgat tttgccagca aatagcgcag aagataaaag
    13081 tttgccgggt atactgagag aacacggtta tggtgtgaca cagacttctg caatggggct
    13141 ggaaggtgag cggctagttc tcgaaatact ttctcctaga aaaagcgaac gtgaactgta
    13201 tcgcctgatc aaagaaatcg aagagcgtgc ttttatcatt tcttatgagc caaaatatat
    13261 ttcaggtggt ttctggacta aaaaagtgag aaaacgacaa ggaaaataac tcacaaactt
    13321 aaaaactgtc taatatctaa attaaatttt aaatgttaga cagtttttat ttattatttt
    13381 gattaaaaaa catcgacata tactcatcaa tttgttatta tactttatat acttatcaat
    13441 ttaatttgga gatattatgg aaattcaacg tagaaccact aattttttac atttaggctc
    13501 tttttttgca actttgacga tcttattttt tctatgttat tcatacctta agacctcaca
    13561 tgcgccttct agtgcattag gctggttgct tttggatcat tctgatatca cagaagccga
    13621 agaaaatccg ttttttttga ttttgtcttc tctttgcggc ttactgtttt ctgtttattt
    13681 cggtgcctat ttttttcatc gacatcctgg tacgttaaac gaagcttcaa gcgtattgat
    13741 tgcatttaaa acgttttctt ttttagcaat tcttttttat tcgtttaatc acaatacgct
    13801 tttcttcttt atcatgaatg gtttagtcgt gattttatcc atttttactt attttatttt
    13861 ttcactcatt tttagccaat tgaaatgtcc actcttcttt cgattagttc ctgtcacctg
    13921 ttttatttac agcttgtttt ccttttttgt ccttgcttta atgccactga ccaacccttc
    13981 gacgatccaa gtcgcaggaa atctgttgga tatgctcttc atcgtcagtc taggtgtatt
    14041 tatgtggcaa agaaaaaaca aacaaaataa aaagcatttt gaagcctgac tattacgact
    14101 tcataaatgc ttttttagtt ttaaacactt caatctaaaa gaataaccca agattggata
    14161 aattattaat gactcgccat tcgtttctta gtgattctgg atcatcaaat ggacttccta
    14221 atctaagcag ctagttcttc ccataaaatg ctatacttga ctgaatcata atattgatta
    14281 tttaaaaatc taacttttct tatcactact tcctttgtca ttccacattt ctcaccattt
    14341 ttttgcattc ctttgtttcc agaccatgta gtgaatccaa taggtggtag ataggcaaaa
    14401 tactcaaata attcttttaa ccataaagga agagaaaggc tcctcctgca agagtcagta
    14461 taatttttgc ttactgctct cttaggtttt actcactaaa actgttctat attctttgat
    14521 ataagaaaat gcatgcggta taaatttctt gctcagctca gaaaagattt gtcgtttaag
    14581 aaaggccgtt tcctccattg tcaatgaagg atccaatgtt ggtaattcaa tctcaaaatt
    14641 gtttttttcg acaataggta attctaatgt gaatgattct tcttccttta tagtattcgt
    14701 cacaagtaga gactgatgga aaatactagg ccggcgattt ttatatttat tcttacgtat
    14761 cctgtcaacg ataacttcta tttgatcaaa aaagtacgct tcatttacgt ctccaataag
    14821 ttcggctaat tctttgaatg ccttcgttac aggaagctga ttcttctgta caaaaaagta
    14881 attagccaaa gtaagcggga agtgttctat agaggaaagt gtgataaaac ggtacttctc
    14941 atttcctgca gttgccctca gcaattctgt ctctcggctg tttcttactc cataaaaata
    15001 cgcttctttc cgttcatctt cgaaacaata cgcccaaata acttctccca tcgctaattt
    15061 ttgttcatca acagagagtc tcttcaattg ttcagctatg gtttcagcag ccgaagtggc
    15121 ttgttgcaag taatctttag gatcaaatat tggaaagttc aaatgatatc ttcgttcttc
    15181 cctcttgatt aatcctgcat cgatcaatag atcaagaaaa tgggaaaatt tattttgcga
    15241 aaattctttt ttcaattctc ttagaatcgt ttcttgttct cgatgttcat atagataatc
    15301 ggtcaatgaa gaaaaaagcg gatcatcggt cagtttttga taaaagtttt ccttattttt
    15361 tgaataataa tagttcatat atgttctctt ttctatccgt tttattttac ttaaaaggct
    15421 tttacgtaaa aaggaggaag aacaagagtc ataattgact gttattctcc tccatttctt
    15481 cggcctttag tatgttctat gtttcttttt atatcaatcg cttatccatt cattttgaat
    15541 gcttcatact accctttgat tagctaaata cagccgtcaa ttgaggtacg acttgttttt
    15601 tacgtgaaac gacaccttta agcaaagcac ggttgttggt caaagttgtt tggaaagctt
    15661 cttctacttt ttcttttcct ttaccagcaa caagtaattc tgaatcactg tctaagatgt
    15721 ttgtgacaac taatacgaac agatcgtagc catttgcttt catttcatct tccattgctg
    15781 cttctagctc tgcttgtcga tccatcactt cattaagatc cactgtattg acttgagcga
    15841 tacgaacgtt ggaactgccc attgggaaac tttttgcatc caaatcaaga agaaccgcag
    15901 ctgatttatc acttagattt gttcctgctt tcaacatttc aagtccataa gtgtttagat
    15961 cgacttcagc gattgctgca agttcttttg cagctgcttc atcttcaggc gtacatgttg
    16021 gtgatttgaa caacaatgta tctgaaatga tagctgagag catgattcct gcgatttctt
    16081 taggaattgt tacttggttt tctttatata gttttaaaat aatcgtactt gtacatccaa
    16141 ctggttctgc acgatagtac aatggattag ctgtttcaaa gtttgcaata cgatggtggt
    16201 ctactacagc taaaatattt acttcgctga tatctgaaat actttgttgg aattcattat
    16261 gatccacaag catgacatct gcattttctg ctttagctga ctctactact cgtggtgcag
    16321 atagctgaaa gtggtctaat gcgtattgtg tttcttcatt aggtgtacct agagcaactg
    16381 cttctgtttc tttacctaat tcattttgca agtgtgaaaa cgcaatggct gccccgattg
    16441 catctgtatc tggattttgg tgaccaaaaa ctaaaatttt tgacatgtca ttcgctcctt
    16501 tgtgatttct cctatttaat aatagcaaaa aaacggaaag aagcaatact tatcttcttt
    16561 ccgatgcgac ctatcgcgtt aaatattttg tatactcttc tgtatgaagc aatcgtttag
    16621 cattttcgac acgttctttt gtaggtggtt cgatcccttc taatggatat ttcaaaccaa
    16681 gtgtttccca cttgtatttt cccatcgtat gataaggaag aatctctact ctgtctacat
    16741 ttgacaaggt ttgaataaag ttgtctagtc ggatcaaata ttcatcataa tcacttcgct
    16801 caggaactag tacatggcga atccaaaccg gtttgttgat ttttgacaaa tattgagcca
    16861 tttctaaaat attttcattg tcccaatgcg ttagtttttt atgctcttca ttatcgatgt
    16921 gtttgatgtc aaataacagt aaatctgtat acttcatcaa ttcttgaaaa cgtgaaaaga
    16981 aaggttcttc atacgtaaaa ggttttccgc atgtatccaa tgtagtatgc atcttccgct
    17041 cttttgcttt tttaaatagt tcgatcaaaa aatcgatctg caatagaggt tctccaccac
    17101 taacagtaat accaccttta tttccccagt aaggtctgaa gcgttctgct ttatctaaaa
    17161 gttcatctgc tgtgtattct ttccctccgc caatattcca agtatctgga ttgtgacaaa
    17221 attggcaacg catgcggcat ccttgcataa acacgacaaa gcgcagtcca ggtccgtcta
    17281 ctgaaccgaa agtttcgata gaatgaacat agccgattgt tttttcttcc atttaagttc
    17341 acatcttttc tttgatttca ttttaagtac aagttagctg tcgaagcgaa gctttctgaa
    17401 caaaattttt cgcaattgcc gcttcgttat gtgtaaaatt tacagggacg gaacatacaa
    17461 gtcctgcccc tgtaaatcgt ttctttcaaa taagcatact gattattcga ttttgatgcg
    17521 atggctattc aacgtccttt cgacgctaag tcgaaaaact tttttcacga actttttaca
    17581 ttctgtcgtg tgaagttctc atcaagacat ctaattgttg ttctcttgtc aagtcacgga
    17641 atttaactgc ataaccagat acccggatcg tcaagttcgg atatttcatt ggattttcca
    17701 ttgcatcacg caataaatca ttactgaata cattgacatt caaatgataa gctccttttg
    17761 agaagtatcc atctaagaca ttacgcaagt tttcgattct tgtatcttgt tcttttccaa
    17821 gacctgaagg attgattgtt tgggtatttg aaattccatc caatgcacag ccgtaattca
    17881 ttttagctgt tgagttcagg ctagctaata acccactttg ttccgcattg taacttggat
    17941 ttgctcctgg cgcaagtggt tttccagctt cacggccatc aggagtattt cctgtatttt
    18001 taccgtaaac aacatttgaa gtaatcgtca ataatgaagt tgttggtttt gagttgcggt
    18061 aagttttatg acgtttgatt tgtcccatga agtattccaa gatccatgaa actaattcat
    18121 ctgcacggtc atcatcattt ccgtatttag ggaagtcgcc atccacttca aaatcaactg
    18181 ctattccttt ttcattacgg attgctttta cttttgcata tttgatcgct gacaagctgt
    18241 ctgccgcatg agaaatccca gcaatacctg tagcaaatgt gcgttgcaag tcagaatcca
    18301 tcaaagccag ctgagctgct tcataagaat atttatcatg catataatga atgatgttca
    18361 acgtattgac atatagttct gctaaccaat ccatcatatt catgaagtta tgtttcactt
    18421 cttcaaagtc tagtatttct gatgtgattg gacgatattc tggtccaact tgggcgccag
    18481 tcacttcatc gactccgcca ttgatggcat ataatagtgt tttagccaag ttagcacgag
    18541 caccaaagaa ttgcatgtct tttccgacaa ctgttcctga tacacagcaa gcaatcgcac
    18601 aatcatcaga accccattgt tcacgaagct gattatcatt ttcgaattga atcgaagaac
    18661 tttcgatagc gattctgcta gcatatgttc tgaatccttc tggaaggtgt tcagaataca
    18721 aaacagtcaa gtttggttct ggtgaaggtc ccatatttgt taatgtatga agcagacgga
    18781 aatcattctt tgtaactaat gaacgaccat ctaatcccat acctgcaatg gataaagtag
    18841 cccagatagg gaatcctgag aataattggt tatattcagg tgtacgcgcg aatttgacca
    18901 tacgtaattt cattactaag tggtcgatca attcttgtgc ttctttttct gtgattactc
    18961 cattatccaa atcacgttgg atatagatat caaggaatgc tgagatacgt ccgattgaca
    19021 tcgcagcacc attttgtgat ttgattgctg ctaaatatcc gaagtatagc cattggacag
    19081 cttcttttgc atttgcagct ggtttagaaa tatcatagcc atatttcgca gccatttctt
    19141 tcatttgcgc taatgcacga tattgttcag tgatttcttc acgtaaacga atcacttctt
    19201 cactcattgt atgataacca gtcatatcat gatctttttt cttttgttcc atcaaatagt
    19261 cgattccata caatgccaca cgacgatagt caccgatgat acgtccgcga ccgtaagcgt
    19321 ctggaagccc tgtaatgatt ttgtttttac gagcagcacg catttcagca gtataagcgt
    19381 caaatacacc ttgattatgt gttttgcgcc agtcagtgaa aatatgtgtc aattcatcgt
    19441 ttggtgtata gccattactt tttagcgcat tgttagccat attgattccg ccgaacggca
    19501 tgaatgcttg tttcaaaggt ttgtctgtct gtagaccgac gattttttcc aaatctttat
    19561 ccaaatagcc tgcatcgtgt gaagtaatag ttgatacgat ttctgtgtcc atatcataga
    19621 caccgttacg ctcgaattgg atatcattta acttttgcaa ttcatcccat aatttttctg
    19681 ttgcttctgt tgggccttcc aaaaagctgt catcaccgcg atattcagtg tagttatttt
    19741 gaataaagtc tctagtgtct acgccttctt tccatttggt acctttaaat ccattccatt
    19801 gctccattgt atggacctcc taaatttctg ttttatgtaa cagtttttac gtgattagta
    19861 taacacagga ccctatcttt gcaagggttt tcttgttttt tatttaacaa tttttatgtt
    19921 ttatttacat atatttaaat caacgttgac ataccaacat ttaagcattg tgaacacttt
    19981 catctttttt tgcaaacgta taaggattta ttaaaaatga atgaaaaaga gacgccgcct
    20041 gta
//
LOCUS       DAPBAB010000047        19996 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPBAB010000047 DAPBAB010000000
VERSION     DAPBAB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.049"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            4..816
                     /locus_tag="RD444_002004"
     CDS             4..816
                     /locus_tag="RD444_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDT7549371.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="RD444_002005"
     CDS             813..1613
                     /locus_tag="RD444_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDT7549372.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="RD444_002006"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="RD444_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDT7549373.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="RD444_002007"
     CDS             3110..4819
                     /locus_tag="RD444_002007"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDT7549374.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="RD444_002008"
     CDS             5031..9383
                     /locus_tag="RD444_002008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDT7549375.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="RD444_002009"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="RD444_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDT7549376.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="RD444_002010"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="RD444_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDT7549377.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="RD444_002011"
     CDS             11221..11514
                     /locus_tag="RD444_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDT7549378.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="RD444_002012"
     CDS             11511..11822
                     /locus_tag="RD444_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDT7549379.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="RD444_002013"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="RD444_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDT7549380.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="RD444_002014"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="RD444_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDT7549381.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="RD444_002015"
     CDS             complement(14623..14844)
                     /locus_tag="RD444_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7549382.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="RD444_002016"
     CDS             15292..17655
                     /locus_tag="RD444_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDT7549383.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="RD444_002017"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="RD444_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDT7549384.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="RD444_002018"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="RD444_002018"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDT7549385.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3513 c         4389 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcc
//
LOCUS       DAPBAB010000048        18973 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPBAB010000048 DAPBAB010000000
VERSION     DAPBAB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.050"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..174)
                     /locus_tag="RD444_002019"
     CDS             complement(<1..174)
                     /locus_tag="RD444_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549386.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
     gene            complement(341..2311)
                     /locus_tag="RD444_002020"
     CDS             complement(341..2311)
                     /locus_tag="RD444_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549387.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2326..3192)
                     /locus_tag="RD444_002021"
     CDS             complement(2326..3192)
                     /locus_tag="RD444_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549388.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3182..3415)
                     /locus_tag="RD444_002022"
     CDS             complement(3182..3415)
                     /locus_tag="RD444_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7549389.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3427..5583)
                     /locus_tag="RD444_002023"
     CDS             complement(3427..5583)
                     /locus_tag="RD444_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549390.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5603..5824)
                     /locus_tag="RD444_002024"
     CDS             complement(5603..5824)
                     /locus_tag="RD444_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549391.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(5934..6272)
                     /locus_tag="RD444_002025"
     CDS             complement(5934..6272)
                     /locus_tag="RD444_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549392.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6672..10313)
                     /locus_tag="RD444_002026"
     CDS             complement(6672..10313)
                     /locus_tag="RD444_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549393.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPAPDN
                     QENNQETVINKSVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10316..12265)
                     /locus_tag="RD444_002027"
     CDS             complement(10316..12265)
                     /locus_tag="RD444_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDT7549394.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12307..13365)
                     /locus_tag="RD444_002028"
     CDS             complement(12307..13365)
                     /locus_tag="RD444_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDT7549395.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13484..13939)
                     /locus_tag="RD444_002029"
     CDS             complement(13484..13939)
                     /locus_tag="RD444_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549396.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14523..15326)
                     /locus_tag="RD444_002030"
     CDS             complement(14523..15326)
                     /locus_tag="RD444_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7549397.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16306..17529
                     /locus_tag="RD444_002031"
     CDS             16306..17529
                     /locus_tag="RD444_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDT7549398.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17564..18331
                     /locus_tag="RD444_002032"
     CDS             17564..18331
                     /locus_tag="RD444_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7549399.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            18523..18921
                     /locus_tag="RD444_002033"
     CDS             18523..18921
                     /locus_tag="RD444_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7549400.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         5734 a         3620 c         2749 g         6870 t
ORIGIN      
        1 aactctcgct ttttcttcat cagtaaagtt tttatacaac tgattacaat aatgaggttt
       61 ttgaagtttc tgaataccta tactaacttc aaatccttct tcatgttgaa ttaggattac
      121 ttctgtgtct ctacctagaa atattcgatc taaaattttt atgtttggtt tcattattta
      181 acctccacta taattatttc actcaataag gtttcacttt atgaaatttc tatagttctc
      241 ttttcacaaa acgtaccatg ttctctaaga ctgtttttag tctcaaatat acatgttttt
      301 tcttatcctt tccttataca ctcaaaatca aagataaaac ttaaagaata aactctccaa
      361 taacaaaatt attagtcaga tccactggtc tcctgatctg aaatttatta cttcttcctg
      421 ttgtcaaata ctgtctcaaa tcaacagaaa gtttttgatc atcttctcct tttctagcag
      481 gagcaacttt tttttcatac ttcattaact ctccactggt catctttcca atgatagtgt
      541 aattaaaatc actttcaaaa ctttggtaaa tttttccatc tgtattgaat atatcagctg
      601 tttcaatatc ttctttgtat tcattgtctt ctcctgaacc gattgtatca aatagaaagg
      661 caagacgaat tccatcttct tcaagcattg ttaatctttt tttaattagc ccaacggttt
      721 caatagataa cttatcgaag ccgtgaatca taacaatatc atcttttttt gcttgtgatg
      781 tgatgaaatc aaaggtattt aaaaattgag cttccataac attggtttct gctcttagat
      841 cacctaagtt atagtaaaac tgtaatttgt cacttttacc gatttctttc agtgtcgttc
      901 tttgattaaa catcctagag aagctgtcta atgctgttct caatgtgtct tttaatacaa
      961 taatacgatc gacttctttt tcagtaccag attttttggc ttttgtttca gcattagtca
     1021 aatagctaat aaaatcccca agaactggca ttgtatcact actgttcacc aaaagttttg
     1081 aacgttcttc ataattaata tcccaaagtt tctgacttat ataaaagtta cctaattctt
     1141 ttttcaataa aactctagtt gcagaatcta atgttctgcc tgctaacaaa taaaaaatct
     1201 gagttagttt atcgagattc cgtctatata aatcaccaat gtccttttga ttaccaaaaa
     1261 gctccaaaga gttaagacct tcttcagcca aattaacctt ttctattaca ctgtttaacg
     1321 aaggtttggc aacaaatgtt ctaccagttg gatcaccaaa gtaatcaaat ttatttaaga
     1381 caatatgaaa agttcgttta tgatttgcca ttgcattatt agcaatcaat tgcccccaaa
     1441 gtgatgatgc tgagattttt tcatcatagc ctttaattgt agcgtttttg tgtgatgcca
     1501 ctaaaacttt acttgtaaaa gaatcatcca gtgccataaa agctacacca cttgcgtagc
     1561 tagtagacat agtgccgata gcccatccat tcgcatcatc taaacctttg cgaattgcat
     1621 gatcatttcc tgcaaaatcc gaactcatag acgtatactc atagacattc ccgattggtt
     1681 cacttaaaag tctatcaaac agatcacgtt gatctcctcc atatgacata agctttaatc
     1741 catacatttt ttcatcatac aaccgttgga gtttaattag ctgttcgctg attttattag
     1801 gagaatcgga tactaatagg atttctagct tccaatcaat aattttattt gccatgctgt
     1861 ctagtaaaga tgctgtattc aaatccataa tccgcataac agttttgcta atttcagcat
     1921 ctgaattttt tccttctaaa ttattttcat aactattcgt tgatccccca acattctcgt
     1981 gcattatttc ttgttgatct ttttgtgaca ctggcttatc tgcttcaatt agataggctt
     2041 ttaccccttc tactctcact tcaggaatag ctttaaccat ccaaccaaat ccataatttc
     2101 tattcattcc ataagtattc actaatttca caattgttcc ccaccgctta cctgtcctaa
     2161 tgtaagatgg tgtaaattga taaccaacta aagatgttaa atcactaaca aaggtattat
     2221 tcactacctt tgaatttcta gttttttgtt cttttttagt ttttctcttt tttttgaaat
     2281 ttaatagttg tgatctaccc ataatttcca tttaatctct cacctctatt tcagtgcata
     2341 aatatcagat aatatttctt ttgtttgttt agctgacaac tgagtgacac tataataaag
     2401 tcctctactc gtatagtttt ctaaacgttc cacaccttca tccaatacca tcttagattg
     2461 agatttcaag ataagatatt ggattactgt tgttttcaca ccatcaatat attctgatac
     2521 aaatcgttct tgctgatcta ttatgtcaaa gactgctttg ttactccttg catttcgttt
     2581 ttgtattttc atatttcgag tttgtctttc cgtattctga ggttgagatg aagtgatttt
     2641 gatttcagct aaggaacgat ctctggtatt ttgatattgt attgatttcg attcctgagc
     2701 cataacttct tctggaaaag cggttagtga tgtagcccca tctagattga acattaatgc
     2761 tacactccca tctcgatata cgattgttcc atcttgtttc acttttaaaa tctctgtgcc
     2821 tcgaaatggt ttctttttcc ccatagatac tcttgctgaa ttaactactg atttgaacag
     2881 tctaatagaa ccagaaatca atgaaccact agatggatca ataaacatat actggctaat
     2941 tagaataaga aatccaatta tcattaataa cgttggaaca aaaataaatt tcagtttaat
     3001 cgtatcagaa actaagtaaa caattgatcc aagaacaacg actgcaacaa ctatagctga
     3061 tacaattaat ctttttgctg taacagaata ggtgctattg acatgctttt caagccattc
     3121 aactctgaca attgtttcag aaggtttttg atgtaagtta tgtttaaatc catagtttga
     3181 gttatctgcc atcagaaata cttcctttca cgttcaactt acggtaaaca cctacagtta
     3241 tcactattgc aacaataagc aagccaatta aagatattga cttgctcatt gtctcgttcg
     3301 tttgtggtag ttcatttacc tgatacacga gctgtgcttc ctgtaagtgc tgagaagagg
     3361 tcatgttttc ttgattgttt tgaattactg gataaactat tttttcttct cgcataatag
     3421 ccctccttag ttattatcag ataagtgagc ttgaattttg tactgatttc cgtctggagt
     3481 tttcacaact gctttttctc catcaataga aatcacttca gccttagttg ttttcccatc
     3541 aatactgatg acaacatctt tcccaacttt gaactctgta gttggtgttg cttcatagct
     3601 actatttgtt gaagcagagt tcgtatttgt cccaatcgaa ttttccactc tcgaaataga
     3661 tgtttgagtt tgagcctgtg cttgttgatt attatgacta ataaagtagg acgatcccaa
     3721 gatcgttccg caagccattg cagcgacaga aatgatagta gccatctttt tacttgtaaa
     3781 atcacgtttt gaacttgatt gattttctgt agcaactgct aatgatttcg ttttttctgc
     3841 catttgagct agatcacttc gggaacgaga taagtcttct cttaaagctt cactttgttc
     3901 tgccaacttt tcgctattta atttataacc ctctgctaaa tctttgacct ctttcattcg
     3961 atctttatat tcttcgatag ttttttgttg ctctacaagt tttgtttcta attctttcgt
     4021 gttgttagct acctgctttt caacttcttc tttgttcgca aaatatttga caaccacatc
     4081 tgttgttttc tcttttgtat catggaactc agcttcattt tctgactttg ttttttcgac
     4141 aatctctaaa gttttttctt gcacgtctgt taatgatttt tcaatttctg gtttcaatga
     4201 attgataatt cgttccacca caacttcttt tgcttcttgg ctttgtttca atgcatcgtt
     4261 tttaatagat tcaattgctt cttggtactg ttgatcaaga ctttcttcag ttgcttttct
     4321 atattcatct tcttgttgtt taacttctgg taaatgctca gcatcatacc gttgctctag
     4381 tttctttagt tcagcatcca aataagcctg tttcgccata tcataatctt tacgaatgat
     4441 attggatttt tccatcacta aatcatcaat tttagatgct tctgcttcgt agctctcttt
     4501 agcaaccgat agtgtatttg taaactgatt ttgaccattt aatgaggttg cttcctctat
     4561 ccgcaaaagc tcatcagcta acatttgctt tcctttgagg atcaattgct ccttcgtctt
     4621 atttaattca ctgttgattt tattattagc aatagagatg cgagcgttca tactctcttt
     4681 agctaaaaac agcaatggtt tcaactcagg tatggatagc atctcctcgt ttaaatcgac
     4741 agggtcatta atctcatcta aaactacagg ttgaaatttg tcagcaatga tctgttcaaa
     4801 atttttttct aaaaaatcag catctaagtt tttcggtaca tcactttcag atgtgatttt
     4861 ttcagcttca taaacataat tttctttgtc actttgggat tcgggttttt catcaatttt
     4921 tggttgttgg taggtttctg tttgtatttg tgtttcttct atgcttactt cttggttttc
     4981 tgcttcggtt ggtttgttat catccacaaa aagagcttct ttttcatctg gtacattttc
     5041 tgctacggct tcaaattcaa cttcttcaga agagttgaag gtctcagcaa ttcgttctaa
     5101 tccattagta gctacttcta attgctctac tgcgctatag aagtctgtag cttcttcaac
     5161 ttctgattca aacacattag gatcatccgg aataactaat aattgaccac tactatccat
     5221 aaaacggaca cctgctaata gcttctcttt aaatttttct ggtagatcag ctacataatt
     5281 caaacttgac ttcaatagaa cgacatactt ctcagaatcg agaatagcat catcctcatc
     5341 tataactccc tcactgttga gcttttgtac atcttcaatt accatataat ttttctttgg
     5401 taaatctttg gtcagcaacg catcgaattc tttccatacc tttgctttag ctttttttgc
     5461 cttcgcttct ttttcttctt ttttgtacac ttcgagtaat tgtggcttca aagagagtgc
     5521 aactacagga actgtttctg ccacataatt aatagattcg gtaatatctt tgaattttgc
     5581 cattattgta aaactccttt tttcagtcaa tagttctttt ctgtttatct agcatatata
     5641 catatgcttt ttcgaattta ataagcgttt caagtctggc tttttcaatc tttttactcc
     5701 catttcgata atcatagaac tggttaacat tcatattcat tatctttgcc agttcggtac
     5761 ctttcagact gctcattaat acttcgttgg cgtgagctaa cattttttct ctatcaaaaa
     5821 ccatcttaca tttactttct tccctcactt ctaaactacg cttggagcgt agttttttgg
     5881 taaaaagtaa attgaacaaa tcggcatatt atccaaattt gttcaattca cttttaacct
     5941 aacagttcat tttttggaaa ttttgaaact agatagatac caacaactgg aataagtgct
     6001 gatatgatat tagctttttg gttcaaatca ttacctgttt tagtacccat tgtgtaaagc
     6061 ataacggttg taattacagg aataataaag attgcaataa tcgccccaac atattttaat
     6121 ccatctcccc attttgcatc accaaaaaat ttcattgcag tgaatcctgg tttcaacgaa
     6181 aaaacgaatg ccaagaaata caaaacaact tgtactaaag ttttcatatc aggcgtcatt
     6241 ccgctaacca aatttttaat agaatcgaac atataaaaac ctccttttta atatttttaa
     6301 taagttaatt atatcatcga caaatcttat ttgttcactt gtcaatgatc taactaagaa
     6361 aaagacgtct cgatatttac atctttatgt tatcataata acataaattt tcgatgatta
     6421 aaagagattt tccattaaat tatttcactt tttttattgg ctaaaaatgc tggtttatca
     6481 agtttttgtt tagataataa ttctgttctt taccagcata agcccttttt aattatacaa
     6541 ctttgaacag taaatattta attcatttat agacaattta aaataatttt tttgcataaa
     6601 tagaaatttt ccttgacatc ttcagttaga aatcaaagta aaaagaagga gcaaatcaat
     6661 gctccttctt tttactcgtc ttcagactta ttttcagttg atttttgatt accgtatttt
     6721 acaatatagt ccttgactga catggtagat ttcttcacta ctttaccagt tttggatgct
     6781 cctgtagcag tagccttggc aaccttagcc gttcctttac ctattttaat agcttcgttt
     6841 ttagcttcat aggttatccc tgctagacta ccttttggag cttttgctaa agctttatct
     6901 gctgaggtgt aaacattttt accagttttt gatagatatt caacggctga tttagctgtt
     6961 gatgttttct tggttgattg attagatttc cctgaattat ctgaagtaga tttagaatta
     7021 tttgttctat tatttttatt tgtcagttca ggttctctaa ttgattgatt gtctttcgac
     7081 attcctgatc caatatttgt ttcatattta tctgtttgat taacattagg agtaggaatg
     7141 aactttttct tcacatctga ttgttcttta tccgtttctt ttgttagcga ttctctgtct
     7201 ttaagtactg tgtcattccg aatagcacta ttatcaaagt tgctgttaag ctctcttgac
     7261 tgactttcac cagatgctcc atctcctaat tcccgatgca ctctgtctga cacgtcctca
     7321 ccactcattt cattcctcag ttcttgatga gtttgatcta aagtaatctc gtcttcttca
     7381 ttctcaggaa ttgcaacagc aattggcgta tgccgttgtt gttcgagttc tctatttcga
     7441 tctgccacaa gcgcatctct cgtctcaatg atattcccat cctcatcgac taaatctcca
     7501 ttcacattat atccatcgct gtgtttacca agtttattac caaactcatc aacaagattt
     7561 ccatcttcat caactaaacg acctttttcg tcaactaaac gtccgttact atcatgacca
     7621 aaatcaccat ttagcaacct gcgtccattt tcgtcaacaa gatttccatc ttcatcgact
     7681 aagcgacctt tttcatcaat taaacgtcct tcgctatcca taccaatatt accatcttca
     7741 ctatcttcca atggtttacc attagtatct ataaggtttc catcttcatc gaccaaacgc
     7801 cctttttcgt caataaagtt gcctttagca tctttaccgt atttactatc ttttaatatt
     7861 tcaccatttt catcgacaaa gtttccgttt tcatcaacca tacgtcctaa atgatcaata
     7921 tattgttggg agttattacg tagttgatct tgacgaacta aatctttgaa agaggtatta
     7981 ttgattgtgg aaccaactcc gccaccgttg aatgacattc cttctggtgt ttcaggaatt
     8041 actttatcag caatggactt agcatcagca ttgctatctc ccatattgtt caaacgttca
     8101 gccatttttt gcgctccaac tgctccaaga cctcctgcaa ccatatcttt tactgagctg
     8161 ttgccaccat cataataggg atttttagct ccttggcttt tcttgattgc tttagaaacg
     8221 ccgtccacaa ctcgcacagc atcatttcta ttaccaaata ataacttagc acgttcatca
     8281 aacccaaacg ctacttcaag tggtaagcca ataagcatat taatcaaatt atttactttt
     8341 tcgccttttt taaagactaa aagatatcct acacctaaaa tcatagcaga tcgaacaata
     8401 tttgctacgc catcgccaac taatgtatta ggaatgaatc ccacagcagt atcaccaaat
     8461 gcacgaataa aagtaatcag aaagataggt aattgtagtc cgatcagaga tgttgtaaca
     8521 acacctaatg aaattgccat cacacctgct cctgatgcaa gtgatcctgt tagaactact
     8581 tcccaaatcc gaataaattc acgtttaaat gctttcacga tattcacatt gaacatcgcc
     8641 caagcacatg ctaagtaaag aactgctaaa acgattgatt cagcgttcat cgctagccac
     8701 gagccaaact tacccatcgc tccatgacca actaaggatg ctgtacgcca ttctgttttt
     8761 actgttgaca tattcttagc actgccttga attcctgaag gagcgatatt atacgcataa
     8821 aacttagctc ctgatgtatc aagtgccgat tggagcaata aagctaccga ctgtgtgcta
     8881 aaagttgcat tgttagcaac gccccaaggt tgagtagcat caaagttatg cgctgttcct
     8941 tttccatcag gcttgcgatt gtcgtctgtc acattttgat ttgctgacca aatatatcca
     9001 tttaaattgg ttggtttctt aattgcactt ccttcttttc catcccctaa attatagcct
     9061 gtccaaacag caggcgctct tcccatataa gcatcatttt cggcgacatt aacatcaccg
     9121 gcataagtat taatattgaa agtatcattc gatgaccatt cttttaatag agccataccg
     9181 actgaattag aatctagatc attattttgt ctggcataac tgctagtatt gatcgctctt
     9241 atcattgcag attttttaga tggttcataa tctgtatcaa cgaacttgat ttcggatgta
     9301 ggctttccgt caggtacatc accaccagta tcagatgatg gagcaagatt ttgagtagaa
     9361 gcccaaagtc ttgtgttaat gatgtagtca gaaacaacat ttgttggtat atctccatct
     9421 ttttttgctg actggatgcc gttgatataa actcccaata gcgatcctgc cactggtaag
     9481 aatataaaca atacaaaaat acgaacaatc catttttcaa gtactggtga taatcccttc
     9541 gagttaacac catctttcga caataatttc attccttgat aacctagtat tgctgtgaaa
     9601 aagagcatag ataagcttgc tatcgcttta aataattcat tatttaatcc caaattgtta
     9661 agcaagctag ttaatgcttt agataccgga ttagatacat ccgatccacc ttctccccac
     9721 cccataagag aaaatatatt catactcatg aaaaattttc caaggagttc atttatggat
     9781 tcagcaatat tagaaaggat taaactaatt gatccgataa aactaccaat cgtcctacca
     9841 atacttccta gaatggatga tgcttgactg gcaatagcat ttcctgagac tgtttcaata
     9901 tatccatagt aactataagt tgctagagta gatgctagtt gtgtacctgc attcccccct
     9961 gcaatcgaat taccttgttt agaaatatca gaataaggaa aatcagcact gataccacct
    10021 tgtcctaaca aattcatttg tccagtttta gcagcactat cattgacttc tttatctccc
    10081 atcaagatac gagcatatag tgtccacatc ccttgttctg ccggtttact ttccgcataa
    10141 gcaacgctct tatttataac tgtttcttga ttgttttctt gattgtcagg agctggatca
    10201 ttcattccaa atggagcgcc taaagttact gctaataccc ctccaataac tagacctctt
    10261 acgacccatc gttttattct aaggttttgt agtaaatatt tcttgaattt catagctacg
    10321 cccctccaat cgaaaataca atcttgtttt ctttactttc agcaacggaa ggctttgcaa
    10381 taatttcagc attagaatca ccttttcctt caaacgctcc agcttcactc aacttaacat
    10441 cgaatatttt tgcgtttgga taagtaatta ttgcttcagt aatcgcttct tcaaactcag
    10501 attcaactaa atgcaagata ttccttgcac cccctgattt agaatcctct tgtgaagcca
    10561 tgtcaaaaac taagtgcatg gagagatcgt ctgcataaaa attttgacca tatactcttt
    10621 ctttcctagg tggaagacgt aagtctatat ttaaatgttt tcgtactaat tcagcactct
    10681 tcaacatttc attataagca atttccagaa gagtttgttc ggataaagcc caaaatggaa
    10741 ctattcgtcc aaatcggtta ataacttcag gctttagacc ttcaccaatc aaggcttgtt
    10801 tgatccctga tgcgactttt cggtattctc tttttagttc agtgctgtat tcattagaat
    10861 tttccttgtt tgtaacacga gatagtgagc tatatcggtt taacgaatcg aacatctccg
    10921 cacctgcatt tgttgtcgca aataaaattg tatttgacaa tgaaacggtg tcgtgaactt
    10981 ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc ttcatctaaa attgataaaa
    11041 aggcatcgag tgtttctaca ccagctttct catattcatc acacacaata atcgcagaag
    11101 gatcatgcct tacatgagta cctatgagtc gtctaaaacg cattgcatca tctttggttt
    11161 tgtaatccgg catattaact aagagaactt tattttcatc gccataaagt cctcttgcca
    11221 cggctttagc cattgcagtt ttaccgaccc ctgttgatcc tgtaaacaga gcagttgctt
    11281 ttggttgctt tcgaccttta aaacgatcaa atccaagctt catacgttta aacaacattg
    11341 ttatagtata aactgctaac ggttgtcctt ttatggactt taattcctct tgaagttttt
    11401 ccatatcaaa atcagaatct agctgaatat tatacttacc aaatatttgc aatagacttt
    11461 ctcgattata agcatttccg tctactcgat cttttgcaat caaatcctcc attacagcaa
    11521 ttgatttagc cggttcggac aaatcctcac gataaagttt gtttgcttgt acgatttctt
    11581 gaagtaattc atcaggaatc ctttgcgata atctaaaaga agcctgagga tcatctttaa
    11641 cagaggatgt atttagccat tgtctcaaaa tatcgacgac aacatcgctt tttagttcat
    11701 tcaattctaa cggttgtagc cgtctcccta aggcatcatc tcttttgata tagttttcat
    11761 attcttttct tgtagtagcg gtaatcattt gaagtgattc atttgctaac ggttctttta
    11821 gcgaatcacc cccaatttta ttgccactcc caaaagagct aacaacttta tgaaactcgt
    11881 caatgaagag gacaaattca ttatctttgt cctcagcttt taaaattcgc tcatattctt
    11941 tcaatcttgg aattaatgtt tctaaacgtg tttggaggac atcatctccc ccaacacgca
    12001 attgtccgat tgataattta tatacttgta ctttctttcc atcttgtcgc tgtctttcag
    12061 cccattcact tgcaagtgct gttttacctg ttccggcttc acctaacaag atagcttgcg
    12121 gtgtttccgg tctattcaag atcagtgaca actcatggag ttcgtcatca cgtcccacta
    12181 acttatgttt tggtggtttc attggtgtga gagcatgatt aaattcggtt tgaatattct
    12241 taaaaaactc ggtactattt tccatctgtt tgtgttcact tcctctaaaa taagaataat
    12301 ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc ttcatgtctc caaaagaatc
    12361 tttgttaaag tcagcatttt tgtattgatc aatcaatttt tgtgtatcat tgattgcttt
    12421 aatttcggtc tccattcgac tgtctagttg acgaatactt tcttgcgaat ctttatcgtc
    12481 aggattttgt tgtaaacgtt ccagatactc agcttttgtg gcttcaattt tcttgatgtt
    12541 agtatttgaa gcatttaact gatcttctag cttatttaca acattattct tacttgtaac
    12601 tgctacaact tttgaataat caatatcgcc atctttggta agaaagcttc cttgatagac
    12661 attagttgaa tctgaatatg gatttattct gaaattgata ttatcaggag tattaacagg
    12721 ttttgctttt ttcccaaaag aaattactcc ttcattatta acatcattca tagaatattg
    12781 agaaagtgct tttgtaactg aatctgcttc attcacattg actttggtat catcgtcagc
    12841 tgtatcatca ccagaaatta gtgatacttg gtttgcaata aaaacttgat aagctctttc
    12901 ctctaattta ccttttagga ataagaaagc atccccatca gtacttaaca tcccatagct
    12961 catttcaagc ttcttaggac gatctttttc ttttgttgcc atatataagt tatagttttt
    13021 tccttgcgta gataacaact ttctagcgtt ttgggagtaa ccaagtttta ctactgttac
    13081 atctctattt tgatctgtcc acacatctga aatagtgatt tctgctcctg atcgagcaaa
    13141 ctccaatgaa gaccctatag gtgttgtatt gttcacgaga taataattat agtttcctaa
    13201 ttgataaatg tagctaccca agcctgttaa taaaaaaagc actcctagaa aagttgcaag
    13261 cataatgcgt aatccgtgac gtgttctctt atccttatca ccaagcaacc ctccaataat
    13321 actgtattga tctttggtag atgttgaatt gacgtttttt tccatcagtt tgtctgcccc
    13381 ctttcataaa agagcaaaca atcagacgtt ttttcggtaa ttctaggttc gggattttga
    13441 atcatttcgg tatattcttc cccattaata tttaacacaa aaattatctt cctttcttcg
    13501 ttcctttctt ttgctcttta ttttccaaat aatcaatgat ttttgctatt gtaccaagat
    13561 agtaattccc ctcagctttg ttttttcgga ttttgctaac cattggagta ggtacaatct
    13621 cttcatctct caattcactg aaagattttt tacctagata ttttccaata acagatttta
    13681 aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc ttttagttca tggttcatat
    13741 tgtcaacgac ttttgcggtt tcaataattg ttttagttct cagcttaata atttcatctg
    13801 gaattttcaa tcctcgtaat ttcatcgttg tactaactgg taacccatgc tttactgcat
    13861 cagcaatact aaaacttttt agatcatcta gtaagctctc catatagtta acaaattcat
    13921 gccaattaat agcattcatt gtctcacccc ttcatttttt taaaatgagg ttcgggcgta
    13981 acacatttat gttattataa tatcataata acataaataa atcaatcagc aaatttagat
    14041 atcccttaaa gtatcagcaa ttattcaaca agatatttat tttagctatt tcagtcatca
    14101 taaaagacta attagcaata aaataactaa tattatccat ttatcagacg aaaagaaaaa
    14161 acttatctaa ttactctata ttaactttta acaaatagtt gacttgtcca aaaatgttac
    14221 cttcaatctc acaaagatat taagatgttt cctaccaaaa ctgatatcac cggacttttt
    14281 attctgtgtt gtccaaaagt gttaccttta gtcgatcaac aaattaaaaa aaattgtttt
    14341 tttcttgact tgtccaaaag tgttacccat agttgttaaa atttgtatag attagccatt
    14401 ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa acttcttgca tttattgtcc
    14461 aaaagtgtta cccatacttt tttaaagaag caggttgtat taaaattaca accttcttct
    14521 tctcaatctt cttctaacca attatgcatt gaaatggttg gcaagtttga ttctggctca
    14581 ctaaaagtga atctgagccg atcaactctt cttcctttct ttgcatagat tttttcataa
    14641 tcaaaagttt caaatacctg tattccgttt tcatcaggag ctaaaaattc ttctttcgcc
    14701 tgctctagta ttcttttgtc aatatttgtc attctataac ttgaaggtat acttaatgct
    14761 cttctaaaat cttcaacttt gactacccaa taaccatttc gccactcttt atttctgtat
    14821 tgcatgagat gacgatacaa ttcttttgtg tagctacttc ttagttttgt aaaattatca
    14881 agttcaaaac gagtaaagtt tttgccaatt gagtttaaga taaattcaaa ttcctcattt
    14941 gccttaattg taactgtttc cttctgttta tctatccgga atttttgaaa aagccacagc
    15001 tgttcataga cgtctgaatc ttcaaaacgg taggttaaag tacctagttt gtcactcaaa
    15061 gcttttaaca cagaataaaa actgtccttt tctcttctgt cgtatttgac caaataacga
    15121 aagaggttga aatcaaaagt tacttctgct gttccttgac gtttcatttt tgataaaata
    15181 gcccaaaaca catccatttc aacaggctta aatcctcgca aagctgttgt attcagacca
    15241 ttgttataac gcacaattgc atttgtttgt tctgatacta aatctacatc actatcaata
    15301 atcttttcgg taacttcttg agacattgat tactctccaa tcttcttttt gcgctccttt
    15361 aatcatgtta atctggtact aattaaagga aacaattttt tctgcttgtt acctaaatta
    15421 aatcacaact aaaggtaaca gtcaacaaag atttgttact aatctaattt tctatttttt
    15481 cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc tagtgtgata cctcaaatgt
    15541 ccacttttgt tacctttgct gtccaaaagt gttaccctta ctgtccactt ttgttacctt
    15601 tactgtccaa aagtgttacc tttatacccc tttaatactt actctcccaa gagattaagc
    15661 ctcccctaac agatatatta acagatattt aacagatata ttaacagata tttaataaag
    15721 gaggattact cctctcttat ttcaaaatct taaaaataaa aaatcttaaa aaaaactaac
    15781 actatctagg agtttatttt gcttgtgtaa ctttgaataa ttgaaaaaaa ggaaaaggag
    15841 gaagcttcag cttccccttt tatttatatt tataatatat attttaatct ttaaaacttt
    15901 agcaaccttc aaaaaggctt tatatcaatc ttaaatagat tttagggtgg acataaagcg
    15961 atgcgaacat ggacaaaaag cgatgtgaat gtggacaaaa agcgatgcaa acgtggacaa
    16021 aaagcgatgt ggtattccga atatatccaa aaaaagaaaa agtatgtgaa tttatttcta
    16081 aaaactagaa aaatatttgt tttttaattt tggtaatttt tataatttcg tatttttttg
    16141 aaaaggattt ttatattgaa aatgttctaa aatagcgatt tctgctgtct tatcaatgaa
    16201 aattgattaa aaaaataaaa tgtgttttac agagattgag ttatgttata aatttacgat
    16261 acatttctta ttgtatcgta tcgaaaacag gtctatactt attcaatgga atcgtaccag
    16321 ttccaaattt ttaagctcgt accaagctta gaaagaagag gcaatcgtat gaaaataaca
    16381 gaaaagaatg taaacccgaa atttgaacct accgaaagca atctaaaaaa gtttgattta
    16441 actgttactt tatcgaatca acttgtagat agcattgttg aaatggatgc tatgccacaa
    16501 aaaatttatc tttatgctat aggacatctt gaccctaaaa atccgccttt taataatact
    16561 gttaaagtat ctaaaaaggc tttatttgcg ttgatgtctt ctagttcatc ttcttcaaaa
    16621 cattcaagat taaaacaagt tattgaaaaa ttgcatcagc aatctgtcat taaaattcaa
    16681 agaactaaac ctaacggtaa gactgaatac aaagtcatct caccgctaga aattactgaa
    16741 ttcaatgatt tttctgatgc agtatctatt aaatttagta gttcaattat gcctttatta
    16801 acgaacttag aagatcattt tacgcaatat acatttgatc aagtagttaa cttgacaagc
    16861 aagtatgcga tcagtttttt tcaattattc actaaagatt ttaatctgta tgaagcaaat
    16921 agagataact taacatggag tacaaaatca cttgatagat taaaaaatcc tgttttctcc
    16981 attgcagatt tacgaaagaa atttagtatt gagaaaaaat ataaagcttt tcgagacttt
    17041 gaaagacgtg taattaaaga accgattgcc caactgaata aatacactga ttttaaaatt
    17101 gactatgagc ttatcaagaa gggtcatact tatacaaaca taaagttctt gcttacacgt
    17161 accatgtcag ttccggtacc ttataagcaa gaacaaagag accctgctta tttaaaaggc
    17221 gcagaagata aaacagagaa aatgaatcaa tcgtacttga aagctatgaa tagcaaatac
    17281 actaaattac taatcaaaaa ctttttgatc agttctgccg acttatgcga tcttaaactt
    17341 atgagccgac ttcagaataa agtttatccg ctttatgatc gtttatccaa agatagagga
    17401 tttgaatcag ttgtcaatca cctatcttac gttgctactc ataaggagga ttacacaata
    17461 aaaaatactg ttaaatatct cgaaaaatca attacgagtt atttgcctta tgtgaacctt
    17521 tatgattaac aactgaaaaa atgcgtgaag gaaggaaaaa cttttgaaaa aagatgaact
    17581 gacaaagtac aatgaggagt tcttagcaac aattgacatg aaaatggatt taaagtcgta
    17641 tgaactttat caattgctgt tcagttatat ctcacccaca aagctaaaga aaacaggcgt
    17701 agtagattta cctgttaaag cgattattaa ttacatggat gttgatcaaa atcttgaaac
    17761 ggaactaaaa accatagtta acactttgca aagacaagca gtttatgaaa tttcgaatct
    17821 agacaaaaaa tataagacgt atactactgt atctgctatt gatcggattc attgggattt
    17881 agaagagcat agtgtatctt tcagtttttc agaagatgta attcccttaa ttgtctcaat
    17941 caaaaagaag tatccgttct ttgtttttga agacttagca tcttttcaaa aaaaatatag
    18001 catcgtgtta tacaaatatt tggcgatgta ttataaaaaa tatcttatcg ccaaaaataa
    18061 aggagatttt gagaaagctg aaatatataa gaatcccctg attgaaatag ttaaaattcg
    18121 tgagattacg aaaacagaaa atgagtataa aacaatatcg aattttgata gacgtgtttt
    18181 aagggaatca gttgaggaaa ttaatgaaaa atcaatgctg aatattgatt ttgctaaagt
    18241 tagaaaaggc agaaattaca attttgctgt ccagtttaat atttctagtg agtttgatga
    18301 aaaaacttat agagaaaaaa aattaggtta atgcaaaata tatcttactc gtttgaagat
    18361 gcttcagacg tatgctaaga tatatagtgc cttgggagca aggtattaat ctcctttaga
    18421 ggttaattag cttgtgatcg cctatcaatt tttttggtag gcgattcttt tttttgaaac
    18481 aaaaaagcaa aaattatttt ttaattagag aggtgcaatg cgatgaaata ctttttttgt
    18541 gcagtattcg aatggaaagg aaatgaatat gcagttaaat tcccagattt tccagactta
    18601 tatacacatg gtgaaacttt agaagatgct tttgagatgg cgcaagaagt attggaagga
    18661 tacttggtgc ttttagagtt gcaagaaaaa gaaattccat cgcctacgcc tgttcaagag
    18721 ttaaatgcaa gaatcaagcg atctgagaat gttaatttat ttgtcgtaaa agctaatacg
    18781 aaagttgcta aagctcgaaa caagcaaaat tatgatcgaa aatcagtgac tgtggaacac
    18841 tatttaaatg ttttagcggc tgaagagaat attaatctgt cgaaagcttt gaatgatttg
    18901 ttgagagaaa agttgatttg atgtctctca gatgatcata ttaagcaaaa acagcttgtt
    18961 ttaggacgta tgg
//
LOCUS       DAPBAB010000049        17772 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPBAB010000049 DAPBAB010000000
VERSION     DAPBAB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.051"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..380)
                     /locus_tag="RD444_002034"
     CDS             complement(<1..380)
                     /locus_tag="RD444_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7549401.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAFMAVLKMDE"
     gene            complement(467..964)
                     /locus_tag="RD444_002035"
     CDS             complement(467..964)
                     /locus_tag="RD444_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDT7549402.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(1081..1305)
                     /locus_tag="RD444_002036"
     CDS             complement(1081..1305)
                     /locus_tag="RD444_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549403.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(1409..3319)
                     /gene="ltrA"
                     /locus_tag="RD444_002037"
     CDS             complement(1409..3319)
                     /gene="ltrA"
                     /locus_tag="RD444_002037"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDT7549404.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(4103..5308)
                     /locus_tag="RD444_002038"
     CDS             complement(4103..5308)
                     /locus_tag="RD444_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7549405.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(5331..5484)
                     /locus_tag="RD444_002039"
                     /pseudo
     CDS             complement(5331..5484)
                     /locus_tag="RD444_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(5487..6872)
                     /locus_tag="RD444_002040"
     CDS             complement(5487..6872)
                     /locus_tag="RD444_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDT7549406.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(6901..7287)
                     /locus_tag="RD444_002041"
     CDS             complement(6901..7287)
                     /locus_tag="RD444_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7549407.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(7303..7617)
                     /locus_tag="RD444_002042"
     CDS             complement(7303..7617)
                     /locus_tag="RD444_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7549408.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(7970..8449)
                     /gene="rlmH"
                     /locus_tag="RD444_002043"
     CDS             complement(7970..8449)
                     /gene="rlmH"
                     /locus_tag="RD444_002043"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDT7549409.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            8877..10166
                     /locus_tag="RD444_002044"
     CDS             8877..10166
                     /locus_tag="RD444_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDT7549410.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(10205..10810)
                     /locus_tag="RD444_002045"
     CDS             complement(10205..10810)
                     /locus_tag="RD444_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDT7549411.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(11006..11470)
                     /locus_tag="RD444_002046"
     CDS             complement(11006..11470)
                     /locus_tag="RD444_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7549412.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(11492..11812)
                     /locus_tag="RD444_002047"
     CDS             complement(11492..11812)
                     /locus_tag="RD444_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDT7549413.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(11955..13034)
                     /gene="pepA"
                     /locus_tag="RD444_002048"
     CDS             complement(11955..13034)
                     /gene="pepA"
                     /locus_tag="RD444_002048"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDT7549414.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13234..13584
                     /locus_tag="RD444_002049"
     CDS             13234..13584
                     /locus_tag="RD444_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDT7549415.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            13769..14722
                     /locus_tag="RD444_002050"
     CDS             13769..14722
                     /locus_tag="RD444_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7549416.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            14735..16522
                     /locus_tag="RD444_002051"
     CDS             14735..16522
                     /locus_tag="RD444_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549417.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            16509..17711
                     /locus_tag="RD444_002052"
     CDS             16509..17711
                     /locus_tag="RD444_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549418.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         5033 a         3531 c         3184 g         6024 t
ORIGIN      
        1 tcctcgtcca ttttcagaac ggccatgaag gcttcttgtg gctggtttct gccaagcgtt
       61 actttttttc ctgcataggt cagctttcgt cgcagtgcat aagcaatgac agacttcaaa
      121 aatccataag gctttttacc atcaaaggtt tttgtagaca taaaccatgt gaaagccaca
      181 ggaatcccaa agtatttgag aaatgctccc tctatcatag aaagaggggg caagttgcca
      241 agtatcataa ctgcaaagag tgacacgaca aaccatgtca tttgcgtaaa ggttatggga
      301 aacggaagtc taaagtcatt gatggagtac aacactttct caacagacca gatactggta
      361 tagcttcgta ttttcttcat gtaatcaatc ctttcataaa aatggggata gtcttttgac
      421 taccccgtaa acaagaaaat ctgccagtaa gaatgtaccg acagatttaa tagacgattt
      481 caaaaatccc gtgattggtt gagataaacg ttcccgaaag gtctaaatcc cgaccatagg
      541 attcataatc aatatagttt tgaagactgg ctggcacttc gcctaaagca cccgtttctt
      601 caatgtagta gcgtgccacg tcagacatat catcacaatc ggaatgaatg ataatatctt
      661 cttgatgttc gcttagttct tcaatacttg aaaaatgagt gagcagagca gatagctccg
      721 attgtagttc ttctggcaac tccgatacca tttcccatag gcgattgagt tcgctaatag
      781 aagtgtattc gtcaaccgta aagggtaact cgtaatcatg aatggcgtat tcctcatatt
      841 catcattcaa gccgattttc tctttgactt cctcaaagtc aatgggaaag gtaaaccacg
      901 caccgaccaa ttcgccctca ttgtatttgc ctaaattcgc aatatagact tgcatatcgt
      961 ccatatattc acgtcctttc tttgtagaga ttcaaaaatc cctaccgcac ttcgtttggt
     1021 gtaccattcc tttgcggaac ataagaaaac cacttatatt ccacaaaaga acggttttat
     1081 ttaagcacca ataatgcgat tgaatagctc tagtaaaatg tcttttactc cagcagcgtt
     1141 gaagactaag ccaaccgcaa taatcgcaat aattaaaaag ccaatcagtt tgctaaactc
     1201 acgcttgaag ccaagataca agccaatcac aacgattgct aaaagcacca gtgattgagc
     1261 gtttgataga aaccagttat atatggatag gtaagttcct gatattataa atatctcaaa
     1321 cttttccccc cattcacacg gagcgtgcga ctttcaccgc actccgcgtt ccatcgtgtt
     1381 tatattgaaa agtatttatg tagtagacct atatcaagtt taattcttga ttgccagcta
     1441 atactctata tttatttagt cttatgagct ggactttatt tagcttcaat agagctatat
     1501 atttttgtat tgtttcttct gttttagcgt gtattagacg gtgtaccgcc tcttttacca
     1561 aaataagatt tttataatcg tcatttccac cagctccagt tggtacaata tgatgacaat
     1621 gtatttcatt agcttgttga aattcttcca gagttatagc acattttcca tgttgagcag
     1681 aaaatagcga aattcgattg tctgcgtatt ctattgtgcg attttccaaa gataatttca
     1741 ttaactggta catcagtaat ttatcaacac ttagattggt gtggataaat tttcttccct
     1801 ctagagtgta gcaattcgct aaatacacct ttgccattgg ttttctgtgt tggatatatc
     1861 caacagggaa aataggttcg ccctcgccag caacataccg aagcatttta gatttaccat
     1921 atcttgttgc ctcctttgtc gtaagttgtc tgcctgtttt tctcaatctg ccccttttgt
     1981 ttgtacctaa tctgttagtg aagatagcca ttacagctct attcagtttc atacaatcca
     2041 gatttacatt cgttgcaatc tgataataat tttgcatacc taataccatt gaattataaa
     2101 gtcttgtttc ttctaattcc gttttccctt ttgagggaga agcaattttc tttgcctgtg
     2161 ataccagctt gtctttttgg tttttgaatt gtttatcact tatatgtgat ttaacaacta
     2221 acttctttct tctcggtatc attttaatct tgaaaccaag aaagtcggag tatctttttc
     2281 taacatttac aattcttgtt ttttcttgtg atacttccaa ttttaatcgt tgttcaatcc
     2341 attgagtgat tgctattttg gctttttcag cactttcctt gtagcggcag aaaactctaa
     2401 aatcatctgc ataacgaacc atatacattt cctttaactt tgttttcttc atttctttgt
     2461 aagcgttgct tttacatggg ctaccgcttt tattgatatg aaccacatag ttttttgtaa
     2521 caggattttc ctgccattgg ctttctaccc aatggtctaa ctcattaaga acaatgtttg
     2581 ctagtagtgg ggatattatt cccccttgcg gagttccttt ctctggatat gttattgtac
     2641 cgttagtcat tttgattgga gcttttaata tcctttttaa aatgaatatc aagtgcttgt
     2701 cccgtattcc catagcccat atctgtttta tcagcttgga atggttcacg ttgtcaaaga
     2761 aacccttaat atcaaattca atcacataat gtagctggct gatttgtagg cggttatagg
     2821 ttgcttttat tgcattttca actgacctat taggtctaaa accatagctg ttttcactaa
     2881 atttagcttc gcagattggt tctaatactt gttttataca ttgttgtata agcctgtccc
     2941 acatacaagg aatacctaga ggtctggttt taccatttgg ttttggtatt tcttttcgcc
     3001 tcactggctt aggtcgatac ccatgtggac tgcctgcaac aatgtatctt acttttttga
     3061 caactgtttc tgctggcaat ttgccaatat ctttaattat tgtaccgtct gttcctgctg
     3121 ttttactgcc tgcatttgct ttgatatttc gatatgccag taatatattt tctctcgata
     3181 ggataatatc cataagatga gtgaaaatat ctccattctt acttttggca tataaatcat
     3241 caaaggtgtt ttgcatttcg taatattcag cgtgtcttaa atcttctaca cacaatgttt
     3301 catttttatt tttaggcata aggcttcact cctttacttg aaattgagtg agctttttta
     3361 tcttactcgt aatccttatt gaaacaacat aatacttttc taatatatac acgacttgag
     3421 ggcattcctc cattctcatt acaagaattt cttaggttcg ccctctgctt ttcagcatta
     3481 gttcaactgc ttattgttct tcgtcagttt attctcttga tgagaaccca atacctttcc
     3541 tcgttccgat aatcttatct atacatatat atccttaggt cactagctat aagcctgctg
     3601 gcttgatacc gcctgtaacg ttatatggca tttcataacc tatattctta ctttgccaca
     3661 ccagctacga tttctcgtac ctttgcattt ctacaaagtt caaatttaga cccgtacatt
     3721 cgctgtttcg tcagttcctt atagaacatt ctaaccatag atattttata gacccccgac
     3781 ctgttggata cgctacccac ctctggatag ttttcgatag aagatatatg ttcatcaact
     3841 attacggtat ctttcagccg tctttaccga gcttcaaaca ttctgagttt cctcatacaa
     3901 tgcttgtcgg aggctcaggg caggcgtttc agagcgttac ttcatcattc cacctgtcag
     3961 attatcagtt ctcctaataa ccaaactttt tcgttctttt attagtgcct tagcttttca
     4021 ctaaggaacg agtcgcacaa aggttttgtc caaaattcat aaaaatgttc tcctctctat
     4081 attcaatgaa tttgtatttg agttattttt ttgttgttat cacgtcctgt tcttttactg
     4141 actgttgctt caaaatctgc ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt
     4201 ttacaacctg cgtctggttg atttcatcaa gtttaatcgc aacctttaag gtcggggcaa
     4261 cttgatgaga tagccagttc agcgtccttt ggaaggagta aggctctggt tttgtggtta
     4321 gttttaatcg ttcacgattg ttcccaataa accaagccca ttcttcattc agtttccaat
     4381 cagaacgagg tttggaatcg tctttatcta caaaacggat ataccgattg ataattttaa
     4441 aggcggtatg ctctggattg tcatagacga gtaaatcacg gactgcataa taggcacgct
     4501 catttttcaa tcgaatctca aaacggtttt ttacttctgc gtcttcaatg ggaatatcat
     4561 ttttcttgta ctgctcgtag tccttttcat agatacagaa ataaacttca ctttgtaatg
     4621 aaccgatata gagggtgttt cccatacatt ccttttcctc tttgcgtacc agttcgccac
     4681 tgcgatagct tttaaaactg cggaagacgg agatacattc ttcctgttgg cacttttcag
     4741 tgagtacagg gatatttaaa atccctgtct tatcgttaat ggcaaggtca aggcgtttca
     4801 tcacaccgcc agccaccaaa acgtccataa agaactcata ccagcttctt tgttgtgcca
     4861 gaagatagct ttcaaattgt ctgcacccac gacctttcaa ttccaccaga actcctttgt
     4921 ccagttcatg ggagcaaagg acgaatatgt cgcctaaagc ataatgctct gaataagaat
     4981 agaaaccata gtcctcatga agaaaatagg acagtttcag ttgtaagatg ttttcgacca
     5041 cctgctgtac gtctgttgtc ggaaagcgaa ttcttacata atcaaacagc atttcaaggg
     5101 gagcgtcggg attgaagcgt tccagagctt cccaaaggga ctgctgtaaa tcctctgatg
     5161 gcttgacttt tcctgtttca atatcgctta gatactgcct tgtaatacca gtcgcaacag
     5221 ctaaacggtt ttgagatagt ccataagcca agcgtttttc ttttaaatgc tgtaaccaag
     5281 tttgttcatt cagtaaaaat ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca
     5341 tttgacatac gctgaaattt tgtaaatccc ttgtaaccaa aggattttct aatgtttttt
     5401 tgactgtttc ctgtcgattt gtacccccct gttagatacc gaggggttaa gtgctggcgt
     5461 ggctattgcc acaccagcca gcaagatcag tccacacctg cgacttccgc ttcgcacgtc
     5521 gcctgcgtgg actgtctgct gttggataac tttttaattt cctccaagaa atcatatcct
     5581 tttggtacaa ggggagtata aaactctgat atgacacttg ttcctacatc aacatagcca
     5641 cgacctttga ttcgctttaa gaagaaatcc ttttgtacgt cactgccaaa catcatgcca
     5701 tagcccattt cagacatacg acctaaagcc actctgaaat taaactgatc acggattccg
     5761 tcgcctaaat attttgcgtc tggacgttga caagccagta ttagaaagaa gccagcttga
     5821 cgacctaaca tgacaatctg tttcagctta ttcataactg cggtgttttc ttttgttccc
     5881 agcatttcca tgaaagcgac gtattcatca aagattaaga agtgtgccgg gagacctaag
     5941 taagcataat ttttgccagt cttatagttc ttcatctgct tcatttcctc actacgtttc
     6001 atcatttctt catagaatgt ttcaatgcaa gaaagcaagt cttcttttct atagtagaca
     6061 tttgccatca cagaacctaa gtccgcaagg tcagcatttt tcgggtcaag aatatacagt
     6121 tttgaatctg tatgaagcaa ggcttcaatc agtgtcagta taaagtaagt tttaccgcca
     6181 cctgtaccac cagcaatcaa catatgaggg agcttatcat attcccacca tacgtttttc
     6241 attaagcgaa gtttaccatc tttagcttct acttcatcaa tagaaatacg actggctatg
     6301 gtgtcataga gcaaagtata ttccacatag gaatccttta actctttatc cgtcagctca
     6361 cagtacaagc cactctctaa tttcttttcc aagtgtaaga gttggtcttg atattttccc
     6421 agcgtgattt ccacccgtat ctgtatcaag ccatttttaa gtcgataata cattttaggg
     6481 aagtaggtta tcttttcctt tgtacgacca gcactatctt taaagaaacc ctctgttttg
     6541 acctgttcag attcatacca cttgttttca agtatcatct ttgccagttt ttgacggtgg
     6601 taaagttgtt taaccgtatc atagcgaacc cgtttgaata caaacgctac cagcaagcag
     6661 ataagaattg cgacactgaa actgataatt aaatagggaa tgtcaatctt atctgcttgt
     6721 gataggttaa aatcctgcca gttgatctgc tggattgtct tcacatgaaa cagtccgaca
     6781 accagcagga aaacaggcag gagtgacgct atcgtaaaat gaaagactaa atctttacca
     6841 gatgggcgaa tccttttacc acgctgtttc atgcgaaaaa gtctcctttc tacctagcga
     6901 ctatttgtct tgtgtcggtt ctttctttgc ttgtggttga gctttgaatg aactagaatc
     6961 ctttgtcagc acaatatcgt ctgccttgat ataccagtca acatctgctc cttgataggt
     7021 ggcagtagca acggtgtccg caatgggatt gataagttcc acccgtgcgt tataatcaaa
     7081 ctctttcaaa ggcacgctgg caggaatact tacttgaatc atgcgtcctt gtcctttgga
     7141 ttttaagtca taggtacgtt ccttgatttc atctgaaacc gacccgtctt cattttggat
     7201 tctcacttca cgacgtagag cagagaattt caattctcca aaagtcgtgt ctttatctaa
     7261 tacaatgcca tttgctaatc tcatcatttt tcctctcttt ctttattctt ttatcatgtc
     7321 gtcagcatgt aaaaggtaat ttgtaaaacc acgagtgccg attttgtagc cctctgcggt
     7381 aatacgtgga ttgactaact tcacacgttc ctcaaagccg aaatgttttt cgccagcttc
     7441 agcaggaagc accaccacaa tatcatctgc tctttgaaca tcagaataga gattatagct
     7501 tcttgataag acagttagcc gtccgttgat tcttcgctga acgactttat cctcgccagc
     7561 aaattctaaa ttgccgaatg ttttttccat gttgggaatc acaaatttaa gttccatatt
     7621 tttacctatc ctttcttttt tattggctga atgaatgttt gatggtctta aagagtgggg
     7681 aacgaccttt tgattcttga ttttttgttt tcataagttc acttcctttc aaaatcgggt
     7741 aaaaaaatag acacctcatt ttttgaagtg tctacctatt aaatattcaa attttattgg
     7801 aagtatcttt atatcttcac ttttcaagga taaatcgtcg tatcaaagct cattcataag
     7861 tagtaaatta gtagtaaatt gagtggtttt gaccttgata aagtgtgata agtccagttt
     7921 ttatgcggat aactagattt ttatgctatt tttctcttaa aaaatatcat tatttgtggt
     7981 atggagcccc cgcgttgatc cggaaggcac ggtagatttg ctcgacgagg atcaggcgca
     8041 tcaattgatg tggatacgtc attttgccaa aagagatctg agcattgctt cttttcatga
     8101 cggcttcgct gagtcctaaa gaaccgccga taacaaaaac aaggtggctt tttccttgga
     8161 tacctaattg gtcgatttgt ttagcaaaag attcgcttgt gggatttttc ccttcaattg
     8221 ctaagccata gacgtattct tgttctttga ttttcgctaa gatccgttct ccttcttttt
     8281 cttttacttg tctcatttgc gcttcgctta aattctccgg ggctttttcg tcaggaactt
     8341 cgatcaattc gattttcgca taggcattca gccgtttgac atactcattg ataccttgga
     8401 tcaagtattt ttcttttaat tttcctactg aaataatttt gatattcatg ttttctctcc
     8461 tttgatcttc acgctcactt tgtttattat aatcaatttt ttcgtctata tgtttatttt
     8521 gactattttt gttaaaaaga tttccccaac agttatgcac aacatccaca ggtttatcca
     8581 cagaaaacag cgttgtttta ccttatttca tcacattcat taggcaaaca tacgttttat
     8641 aagaatttat ccactttata cacaggctgt ggatatgttt tatcccttat ttttccaatc
     8701 ctatccgcgt agttttacac aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa
     8761 aacaatgatt tttcctaaag tttttcataa aaagttcaaa gtctcaaagt agactaaagt
     8821 caacaaataa gactatacta acagtagata aatgaatgta attggaggag gaacacatgg
     8881 atagaaaaaa tgtgacacca aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt
     8941 tgggtctggg gcttgttgga gggatcgttg gaggactact gacagctggc attttttatg
     9001 cagcgatggg cactggcaac aatagttcta ctgctacaag cggcaatcaa aattctgccg
     9061 gtgaaacagt tgtggaaaat gtcaaagtca atgtagattc tgatatcacc aaagcggtag
     9121 ataaagttca agacgctgtc gtttctgtta tcaacttaca aagtcaaaac caatcaagcg
     9181 gttttggcgg attattcggg cagcaagaag aaagtagcag ctcagatgat agtaatttgg
     9241 aagcttatag tgaaggaagc ggcgtaatct acaaaaaaga cgggaatact gcttatgtag
     9301 tcacaaacaa tcacgtagta gatggacagc aaggtttgga agtcatgatg aaagacggta
     9361 cgaaggtaaa agctgaatta gttggaacag atgcctattc agatttagcg gtgttgaaga
     9421 tcaattcaga caaagtcgaa acggttgcct cttttgggga ctcaagtgct ttgaaggttg
     9481 gtgaacctgc gattgcgatt ggttccccat taggatctga atatgcgaat tctgtcactt
     9541 caggaatcat ttcttccttg aaccgacaag taacaagtac gaatgaatcg aacgaaacag
     9601 tcaatatcaa tgcgatccaa acagatgcag cgatcaaccc gggaaattct ggtggtcctt
     9661 tagtgaatat cgaaggacaa gttatcggta tcaactcaag taagatcgca agtacttctg
     9721 aatcgacttc taatgtaagc gtagaaggaa tgggatttgc catcccaagt aacgatgtag
     9781 tgaatatcat caaccaactg gaaaaagacg gaaaagtgac acgtccagct ctaggtatca
     9841 caatggtcga cttatctgcc gtttctactc agcaacaaga acaaatcttg aagattcctt
     9901 catctgtaac taacggtgtc atcgtgacaa atgtccaaac agctacaccg gcagaaaaag
     9961 ctgggttgaa acaatacgac gtcattacta agatcgatga caaagaagtt tcttctggtg
    10021 tagaacttca atctgctttg tataagaaaa aagtgggaga ttctgtcaaa atcacttact
    10081 atgaaggctc gaaagaaaaa acaactactg ttgaattgac gatcgatcaa tcggcattaa
    10141 aacaaaatca aaatagtgaa aattaataaa aaagaggttg tgacaaagac ttgtcacaac
    10201 ctctttattt tggaaatact tctccgacta cggcatcctc aggcaatact aagattcctt
    10261 ttttttctgg agcatttggc aaatgcaatt cacgagcaga acaaatcatt ccatagctgg
    10321 ctacccctcg caaagttcct ggccagatca tcattccatc tggcatcatg gctcctggtt
    10381 ttgcaacgac aactttttgt ccagcttcga tatttggtgc accgcaaaca atttgcaata
    10441 ctgtcccatt gtccacttct gtctcagtaa tcgataaatg gtcagagtca ggatgcggga
    10501 cgcatgtttt tacatacccg acaacgatct tcggttcatg atcagcttca agctgttctt
    10561 cgaatcctgc ttctttaatc aatcgattca atttcgctaa ttcttcatct gttaagctta
    10621 cttggccgtt cccttcaatt gttagatgat cggagatatg gaaaaagttc caagcaacaa
    10681 cttgaccttg ttcatctttg atccgagcaa cattccattt acgttcgaca gtgttttctg
    10741 ttcctttatc gtcggctatg ataaccatca aagtgtctcc gacgtgtgct ttgttataag
    10801 caaaaatcat gttctttcct ccattatcct ttgctgagat ttatcttaac aaaaaattgc
    10861 attcactgca tgagattttt ttcatgcttt atttccgaag aagcttcttc tggaacacca
    10921 tttattcgta acaagggact gtgacaagac ttttgtcaca gtccctttac tgtttgatta
    10981 aaaaggttat acaagttcca cttgcttaat tactcggctg ttcttcttcg tcagaaacga
    11041 tcaagacatc acaaggtgaa tgacgtatga cgtagctggc tacacttcct gtcataaagc
    11101 gttcgactgc atttagtcca gactgcccaa ccatgattag atcgattgca tatttttttg
    11161 gcaatttgac tgccatggat tcacgaacag agccgtatgc aataattcct tctatatttg
    11221 tgtaatcaac agattttcca tattctttac attcttcgat catttgcttg gctgtttcag
    11281 tttgctgatc gactagattt tgatcaatag gagcatagcc cataaaatta taaaattgct
    11341 gatcgattac gtttgctaca tatactttcc cattattacg gcgagccact tctaacgctt
    11401 ttttgaaagc agcaagtgct tgttcactac cgtcaatacc tactaaaatg ttcttatatt
    11461 cttgattcat cttgacccct cctaacagat gtcagccttt tattacactt tctaggaacg
    11521 attcgatttc ctgttttgtt ttacgatctt tattcactag acgacctaat tcttttccgt
    11581 cttggatcac gacaaaactt gggataccaa aaatgttcca ttcagcggct acatctatgt
    11641 acttgtcacg gtctacttca ataaattgcc aatcagaaaa gtcattttcg atttcaggca
    11701 tttgcggttt gataaaacgg cagtctccgc accagccggc agtaaagaaa aatacacttt
    11761 ttccagtttc tacgtagcct gctaattctt ctaaactatt tgggataatc attctttttc
    11821 ctctactttc tgtttatgta tttttcaatt aaattataca cctttctttc gaactaacaa
    11881 acattgatcc tctttttata aaaaaaatct gacaaaaggt tgcttttgcc agattttata
    11941 gtttcactat cgcgttattt cccgtatacg atcgtgttca ctgttgattt atctaaacct
    12001 ttcaatgttt gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt
    12061 tgatgcgtat ggatataacg tcccactacg ccaatcactg tacttgggat tccttcattt
    12121 tgtgtatgag cagcacctgc atctgtaccg ccttttgaaa caaagtattg ataagggatg
    12181 ttgtttgttt cagcgatatc tagcaagtac tcacgcaaac gaggaagcat gatcaaacct
    12241 ggatcttgga tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc
    12301 actgtgtcat ctgctgcaga gcagtctact gcaaagaaca gatctggctt gaatttattg
    12361 acagaagctt tagaaccacg caagcctact tcttcttgga cattcgcgcc tgcaatcaat
    12421 gtatgaccta atgtttcatt ttgtaacgct tctaaagctt ctaataccat tgtacagcca
    12481 tagcggttgt cccacgattt gctgatgata ttcttcccgt tggctgtttt gatcgtttca
    12541 gcgtaaggaa cgattgtgtc tcctggaaga acaccgtaac tcattgcctc ttctcttgat
    12601 tcaaacccag catcgaacaa aatatctgtt acttctactg atttttgtcc gcttgttcct
    12661 cgtaataaat gtgggggaac ggaagaagaa atacatggat aattcttacc agtagaggtt
    12721 ttaagtgtaa aacgttgagc agaaacaacg taagggttcc atccgcctag aggtacgact
    12781 ttaaaaagcc cgttttcttg gatttgtgtc agcatgaatc cgacttcatc catatgtgct
    12841 gcgaccatta cacgaggtgc atccgcttct tcatggtggc gcagcccaaa aatgccgccc
    12901 aaaccatcta actgtaactc atctaccagc ggttccatat gactttccat ataggcgcga
    12961 acgtcttgtt caaaaccgct tgttccttgt aattctgtta actctttgat tcgttgaaat
    13021 gttttttctt ccatatgcta ttcgtctaaa tttcagacta aactcctctc agacttatat
    13081 tgatctgcgg ataaaatatg attttttctg cagaccgatc gtatttatta tagcttattt
    13141 tttctggtag cggttattta tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa
    13201 gagtagcaag tgagaagaag gaggtatatc cacatgtatg aagaaagcga attaagctat
    13261 tttaaaggcg ggttagccgt tgggatcggt ctaggattat tgagtggggt agcttcaacg
    13321 atttggtatc atcgcaaaaa agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca
    13381 tcttttttaa aagaaggcgc aatcgaaggt gcttggatct cttttgaaaa agaacctatt
    13441 cggaaattcg ccgttcggtc gagtgcttat cggggtgggc tttcccggat agaagatggc
    13501 gaaactgttt attatgaatt tttagcagat gctcatacag gaaccgtatt agacatttca
    13561 agaaaaaagg gcgcaataga ttaatacgtc aaaaaaatga aataacgaat acgcacccac
    13621 tatctaaaag agaggtatcc tatccttctt ttatagtggg tgatttttat aaaaataggg
    13681 ctatttcctc ttggaatagc tagtatttgt agttattata cggtattctt gaaataagat
    13741 tagttttatt atataaggag agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat
    13801 tccttgttat aacgaagagg cgacaatttc aactgttatt gaagatttta aaagggaatt
    13861 accagaagca gatatttatg tctatgataa taattcgacc gatcgaacat acgaattggc
    13921 cgttcaaggc ggagcaatcg taaagaaaga accacgtcaa ggaaaaggga atgtaatccg
    13981 tcaaatgttt tttgatattg atgccgatta ttatttgatg gtagatggtg atgatactta
    14041 tcctgcagaa gctgcacatg gtttgcttga gaaattacgt tctggcgaag cagatatggt
    14101 cattggcgat cgtctatcaa atggaaccta ttttgatgaa aataagcgtc ctttccatga
    14161 ttttgggaat aatctggtta aaaatacaat tacttactta tataaaacaa aaattcgcga
    14221 tgtcatgacc ggctatcgag gattcaatcg aatttttgtc aaaagcttcc ctattatgag
    14281 ttctggtttt caaatcgaaa cagaattgac gattcatgct ttagataaaa aattcaagtt
    14341 cgttgagatt ccaatcgatt atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac
    14401 attctctgac ggattcaaag tgatcatgat gatcatcaaa atgtgcaaag attataaacc
    14461 tttgcttttc tttgggatct ggaccatctt cttcttttta tttggattgg tcatggggat
    14521 tcctgtgatt cgtgaatata tgctgacaag ctttattaca cgcatccctt ctgcgatttt
    14581 aagtactgga ttgatgactt tagcccttct ttcattagta acagggttga ttttggatac
    14641 ggtcgtcaca aatgccaaga aagaatatga attgaattta taccatgtct accacgaata
    14701 tcaaaataaa gaaatgaagt aggaggcctt ttttatgcat aagaaaaagg agaatctatt
    14761 tcctactttt tttgcaactt tatttgcgtc aggttttgct ttttttactt cttatggtct
    14821 aggaactttg aatccaacag taatcattgg tttagccatg gttttgctac ttgcttttaa
    14881 gcaatatgga atagagttgt tttcaataaa aaacaaagtt gcagctattg cccgattgtt
    14941 tttttctgca gcttttgctc ttacactttc tattggcaga cagatacaat ttcagtatgt
    15001 ctcagcaaac tatacaaaga caacgattgc tgatggaagt attttttact ttcttgttta
    15061 tttctccatt tttgtgtatt cttttttatt tttacaatcg atttatttat caatgccaaa
    15121 actggaaaca ttttttactg ctcaaaacac acaggctttc tcttcaagaa aatatttttt
    15181 attctttact gtctttttag ttctgtgttg gcttccttat ctgttggcat tgtatcctgg
    15241 agtggttttg cctgattcgt taagttcggt cgcccaatct atgggtgatg ttcctataag
    15301 caatcatcat ccagtcttat tcaccttact ggtcaagcta tttgttcatg atttaccaac
    15361 tgatacgatc aatcacggtg tatttttatt ttcgattttt caaacattga taacagcttc
    15421 agcgataagt tattctcttt gctggctgtt aaaaagaaac atccattttt ttccggtact
    15481 cattagttta tgctattttg catttgcacc ggtttttcca atctacgcct tgaacatgca
    15541 aaaagatact ttgttctcta tcgcttgctt tttattttcc ttagcggttt ttgactttat
    15601 ttttcaacga gaaaagccaa cgattctcaa tcagatttac gtaatcatcc ttgctttact
    15661 gaccacctat ctaagaaata atgggattta tgtagtaacc ggcacaggtt tgatttgcgg
    15721 cttgattatt ttgaaacaaa aaaggtatca agcattcttt caagtggtgc tgggttatac
    15781 agtgattgct ggactactca tccacccgtt gatggctcgt tatgctattc ctacagaaac
    15841 tgctgaacaa ttaggtgttc ctttgcagca aatttctaga actattgtta tggatggtga
    15901 cttgtctaag aaagataaag cattcatgaa tcagctactc ccgataaaag attatgaagc
    15961 gtattctcct agtcttgcag actcaatcaa gtggcataaa aattttaaca atgagttttt
    16021 gaatacacat aaaaaagaat ttctatcact atgggcaaga aatttaccaa gtaatataaa
    16081 aatctataca gatgcttata ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa
    16141 tagttacggc tttttagata ctcgtgtaaa cacgaataat tatggtatga agcaagtaga
    16201 tttgattgaa catcttactg gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt
    16261 ctttggttcc ggcacattac tgtggttagc tatcggcagt gcacttttag caatctttaa
    16321 gaaaaaatat cggtatttac tcctttttct tcccggtatg cttgtttttg ctactattat
    16381 gattgctact cctgtggctt ttagtttacg gtatgtgttt gttttagcat taggtctacc
    16441 tgtttatatc ctctctcctt ttttactaga taaaagagaa aaaatgaaaa cagatatagg
    16501 agatactcat gaaactattt aaaaagatat tttttgcttt tgttctcttg atcgctagct
    16561 attgcgtact actgacaatt acttttttca ttccgcagca ctctattgaa tcgaatgcag
    16621 aaaaatctct acagatggtt gaaaaagaag gaatgtatcc ttctatcaat caaggaaata
    16681 tgctaggcac tcggttagat aattttactg atcatttgat gatacgaaaa acaaaagcag
    16741 atcctgaatt gaactccttg gagaacgcga tgtctatggc ggattatccc cgttattggc
    16801 atggttatca gcttttttta cgacctttac tgctagggat gagtttgggg agtattcgaa
    16861 tgatttacgc agccattctt tttctgctga ttggattgac gagctacttt ttaaccaagt
    16921 gtagcgacat ctatttaacg acagccttgt tgatttcgct tgctatagga aatgctgcaa
    16981 cctttttctt cagtatgcaa ttttcaaata tttggatttt gactttattg tctgtattac
    17041 tatttttaac aaaaccacaa ctctccaaca ccaaccaaca attattcttg tatttctttg
    17101 tcattggttc tttagctaac ttttttgact tgttgacaac accggtcatc tcttggggga
    17161 tacctgtgat cattatttat tatgtaacaa ataagtattt aatggataaa cgcagttcat
    17221 tgagcaaaca agtaggcagt tttgttttta ccggtatctt ttggggatta ggctatggtt
    17281 tgacatgggt ggctaaatgg atcctatcat cgatcatatt gaacaaaaac attgtaaaag
    17341 atgcaatcaa ccaaatcctc tttcgaacgg aaggaaacga caaatacccg ttgcatcgat
    17401 tagagatgct gaaaagcaat attagattga tgtatccaaa agtagcaata ttattgctat
    17461 tgattacttg tcttgttttt ctctattttg cttatcgtaa acgaaatagc tggcgtccgt
    17521 tccaacttct agttttgttt gttttagctg ctgtaacgcc gtatatttgg tacaatattt
    17581 tagctaatca ctcacagatt cattattggt ttacgtatcg tacgcaaatc atcacaagtt
    17641 ttgctatact tagtgctttc gcctttttga tccctcctga gaaatcaaaa gacgaactaa
    17701 attactttta agaataccga tgtactaatt catttttaaa cataaaaact ccagctgtca
    17761 gagatagccg ga
//
LOCUS       DAPBAB010000050        17764 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPBAB010000050 DAPBAB010000000
VERSION     DAPBAB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.052"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(46..756)
                     /locus_tag="RD444_002053"
     CDS             complement(46..756)
                     /locus_tag="RD444_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549419.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(774..1270)
                     /locus_tag="RD444_002054"
                     /pseudo
     CDS             complement(774..1270)
                     /locus_tag="RD444_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1307..1759)
                     /locus_tag="RD444_002055"
     CDS             complement(1307..1759)
                     /locus_tag="RD444_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7549420.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWSFVKKGNKRIY"
     gene            complement(1894..2499)
                     /locus_tag="RD444_002056"
     CDS             complement(1894..2499)
                     /locus_tag="RD444_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7549421.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(2949..3569)
                     /locus_tag="RD444_002057"
     CDS             complement(2949..3569)
                     /locus_tag="RD444_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7549422.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3571..4440)
                     /locus_tag="RD444_002058"
     CDS             complement(3571..4440)
                     /locus_tag="RD444_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDT7549423.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4463..4870)
                     /locus_tag="RD444_002059"
     CDS             complement(4463..4870)
                     /locus_tag="RD444_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDT7549424.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(4889..6178)
                     /locus_tag="RD444_002060"
     CDS             complement(4889..6178)
                     /locus_tag="RD444_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549425.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6205..6507)
                     /locus_tag="RD444_002061"
     CDS             complement(6205..6507)
                     /locus_tag="RD444_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549426.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6532..8625)
                     /locus_tag="RD444_002062"
     CDS             complement(6532..8625)
                     /locus_tag="RD444_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7549427.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9114..9842)
                     /locus_tag="RD444_002063"
     CDS             complement(9114..9842)
                     /locus_tag="RD444_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDT7549428.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(9987..12608)
                     /locus_tag="RD444_002064"
                     /pseudo
     CDS             complement(9987..12608)
                     /locus_tag="RD444_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(12722..14053)
                     /locus_tag="RD444_002065"
     CDS             complement(12722..14053)
                     /locus_tag="RD444_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7549429.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(14019..15497)
                     /locus_tag="RD444_002066"
     CDS             complement(14019..15497)
                     /locus_tag="RD444_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7549430.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(15802..17637)
                     /gene="tdc"
                     /locus_tag="RD444_002067"
     CDS             complement(15802..17637)
                     /gene="tdc"
                     /locus_tag="RD444_002067"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDT7549431.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         5052 a         3809 c         3017 g         5886 t
ORIGIN      
        1 aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc
       61 tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt
      121 ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc
      181 aatgaccagc accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca
      241 gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc
      301 cggagcatcg gaatctgcac ctaaaggcag gaatgattcg tcaccttggg cttttcctaa
      361 atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa
      421 ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt
      481 tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt
      541 aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta
      601 atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt
      661 tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac
      721 ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat
      781 aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg
      841 ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa
      901 acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt
      961 aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct
     1021 aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc
     1081 gcaggctctt gatcgatact cactaggaaa aaatctgttt ctttatcttt tagctcttct
     1141 gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtaattat
     1201 cttttccaaa tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg
     1261 aaggcgtcat ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt
     1321 tattgccttt tttgacaaaa ctccaatctt cgctaacatt cttgcatact ttatttaaaa
     1381 gatattccaa ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt
     1441 agttgttttc tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt
     1501 tcttgatttt tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt
     1561 tcttgattgc tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt
     1621 ggatgattcc aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat
     1681 cgacttcttt gaattcgata ttgctaatag agtctagcgc tcgggcaatc attccgatgt
     1741 ctcgaaggat ttcagccatt tcatcccacc tttattttgt ttgtaagact agtttacctc
     1801 tttttgttgc aagtgcaact atttttaata aaaaaatcaa gttctttttt aataactagt
     1861 aaagtatatt cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt
     1921 ttctaaaata agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc
     1981 tatactaaaa aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc
     2041 gtggatcgca caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg
     2101 tttattcgta tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc
     2161 atagacttct ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg
     2221 aagtgaccat tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat
     2281 ataggcacga acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt
     2341 tatttcttcg aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat
     2401 ctcctcaaaa cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag
     2461 tgcgagtagt cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc
     2521 aaatgtatga ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa
     2581 tgaaatctgc aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc
     2641 tctggggctg tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccatcaa
     2701 acgaaaaata tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct
     2761 ttttcacaac ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa
     2821 tattcatcaa acatgagaag ctgtttcttt gaaattgctc cttgtgactc ggagctgaac
     2881 gacaggctca cgacctctcg gtaaacggtg ttcaaaagcc aatttttcga tattaagcca
     2941 tttctttgtt acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa
     3001 tccacttgag tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca
     3061 tattttcggt cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc
     3121 gttcgatctt ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga
     3181 tctgattcac gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg
     3241 cgaatcgtga tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc
     3301 ttatcactaa aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca
     3361 agtgctttgt aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa
     3421 ttgttaaacc cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac
     3481 atatctggaa atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta
     3541 ccagccagaa gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc
     3601 caagttcata aacagtccaa tcttatgctt gacaatttct tttactgctt tatttgctgg
     3661 aatcacatta tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac
     3721 tttcgtccag ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat
     3781 caattcagga taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc
     3841 aactactgca tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg
     3901 tccatgattc gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt
     3961 cactgcgtct tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc
     4021 tgtaccgccg attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc
     4081 tactactgct tttgtcttca agatattttc tttgaaaggt agatgggacc cgtcatacat
     4141 gacagaagta tatccgctat cgattgcttc tttgatatca tcaaagtctc gggcgtggtc
     4201 taagtgtaag actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa
     4261 cacatgcatg ccgatatatt tagcggtatc gacacttgtt tgaataatga ttggcgaacc
     4321 catttctttt gctgcgccta gcatggcagg aagcatttct aggttatgcg tattaaaagc
     4381 tccaactgta aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat
     4441 cttttattgc caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt
     4501 gtcgcatctc atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag
     4561 cagcatctgc acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg
     4621 tctgtttatt ttccaagctc ttttctaaat attggatggc ttgatcgttc tcatgcaatt
     4681 cagaaaattt aagtccaact tgttcataaa gttccgctaa tttatgccta tcttgggtag
     4741 tggaaatctc ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg
     4801 atagggcttt ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa
     4861 atccaaacat cattttccct actttcaatt aaaacgcttt caaaactggg cttaagatcc
     4921 aggcatataa gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgatgaatc
     4981 ccatcgaatt aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag
     5041 caataccgcc aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg
     5101 tccctccggc aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg
     5161 gcaaagtaaa catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacgg
     5221 catttgggct ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta
     5281 atttcatagc tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag
     5341 ctagcaatat atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat
     5401 agttttgatc tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga
     5461 tatatagcgg gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat
     5521 gtgtcggcaa cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct
     5581 tagcaacacc ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat
     5641 ttgatccagt gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac
     5701 aagctccgat aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc
     5761 caaagactgt cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact
     5821 tccattttgt gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg
     5881 caatatctct acctaaaccc aaatcattca ttgcttgatc attgatagat tcaatagaag
     5941 ggatgatccc ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc
     6001 caacgatgat gctcgaaccg gcactcaata cttgaaatcc taggagtgtt tttaacgtgc
     6061 cagagatgac ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg
     6121 caatggtgat cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgctcatgt
     6181 ttttaccctc ttttcttttt gtatttagat aactccctta tcttgcagaa ctggggtgat
     6241 tttttcttca atttcatttt ttgaaacaat atttttcaaa tagatcatcg ccgttttagg
     6301 atcaactgcg aacttttcga actgcgaacg aaaattttct gctgtgatga tcacatctgg
     6361 tttcatggaa actgcggaag aaatatcaca atggtccagt ttggcttcta ctcctaattt
     6421 gttcaaaaca tcctcagcag acatctgtgc tgcaaaacta cttcctaaac ctgcgccgca
     6481 tacgaataaa atgttcaatt tgttcatctt tgttctcccc cgtatcattt attatttctc
     6541 aactactgaa aaaagcagtt gtttgaaatc ttcgatattt gtgcattcaa tcaatcgatc
     6601 caacttctct tcgttgttga tcaattcaat tagttctttc atgatgttca gatgggagta
     6661 ggagtctact gctgccaaac aaaaaataat ctttactgga tccatttcag gattaccgaa
     6721 atcgattggt tgctcaatcg tagctacgct gactcctaat ttattgaccc catcttctgg
     6781 tcgggcatgg gctagtgcca tgtgtttgcc aatcacgata tacggtccgt aatcattcac
     6841 tgcacgaatc atcgcttcga catagcgctc ttcgacaatg ttttctttaa ccaatggtct
     6901 agcagctagt tcaatcgatt cctcccaagt agtggctttt tctttgatca tgatattgct
     6961 gtccgtcaaa atatctttta acataggctg gatctccctt ttgttgattt ctaaattgtt
     7021 tgtttcgaat aatttttcta acttgccata gatgattcga tctactcggc caccactttc
     7081 tttcatgaca tctattattt gagtgaataa ctccgtcgat tcatgactgt taggctctaa
     7141 ccgctgtagt tcttcatttc ttttcaaaaa gtcttctata accaagatat tcgtctcagt
     7201 taaaatcgga tcgacaacta aaagcggctt atttcgataa ttcaattgaa aggtgctgaa
     7261 aaccagattg acatccaatt tatctactaa ttcgatttct tttgaactta aaactgcagt
     7321 aatctcgatc tgaggaaact tttctttcag attctctgct aacagatttc cagtagccat
     7381 cccatggtta cagacaacga ctgatttata aacataattc aagtctcgct tgattacact
     7441 ggcgatcgtc gaaaagtgga tgacaagaaa agctatctcg tcctccagta tcttgccacc
     7501 aattacttct tcgatcgtag gaacaaaatg agtgactgcg cggtagattg ggaaataatt
     7561 tgttcggatg ttttctttca atggattcat gacttggata tgattcttga ttcttacaat
     7621 cagtgcagcc atgtgcttat agagattttc gaataaggct tcttccttta gatggaaagg
     7681 tacgtgtgtc tcgttctcta caaattggat caattggatc gttagtaatt gagcttgtac
     7741 ccactcgatt gaattggaga tatctctagc attgaacgtc tcaatagtga aacgaagata
     7801 atttctttct acttgcggga cattgtccaa ttgaaattcc tcgatcatgt ggtcaataaa
     7861 tgcagaaaag ctactgtctt ttatttctgt atttttccaa ttaatggcag agataaattc
     7921 atgtttcctg attcgctgga tccagatcaa tgtaaaaagc aggatttgtt ttttataaat
     7981 atcatcttct tgtttcaaca acttattgga atagatctcc tgtatttttt tgatttcctc
     8041 caaaggaaaa taatggagaa agattttttg agccggtgtt aattgtgcat cttgattcat
     8101 aaagaagtcg gtcattccca gattctgatt gataaaatca tagatcatcg tacggataga
     8161 tcgttctgaa cctttataaa ccagtccttg ctttccataa ctgacgatct ctatcccata
     8221 actgaccagt gttgctcgta accgtctcat atcttcatct aatgtacttt tagagatttg
     8281 aaattcttct tcttttttgt ttagatggat cgggattcgg ctgtaagcaa cggctaaaac
     8341 tagatcaaaa atacgttcgc gccgattcaa aaagccgtct ttcacatctt ttatttcagt
     8401 ttctaatcga gcttgctttt ccttttccag cttcaacaaa aaacctttgt tgcgaattgt
     8461 acggatctcc ggaagctttt tactgctcaa ccagatattc gcttcaagaa cttcctgacg
     8521 aatcgtccgc tgactgacat tgaactcact tgctagatgt tttgctttta ctggatattg
     8581 ggctgtcatg attctgtgaa gtatttcaat tgttcttgtt ttcatagaaa tgatctcctt
     8641 ctgatttcaa gtatatggtt ccgcttacat aataaaaagt agatatttct gccacttgaa
     8701 gtggaaagaa agtaagcaaa gcaaacaaaa aataagccat atgctttttc atctcctcaa
     8761 cgaaaaagac atacgcctat tattacaaaa attttgccca tctatacaaa aaagaggttg
     8821 tgaaaaagct gtcctgcatc aagtaataag aacaactaaa ctaaaatagt gcttcctatt
     8881 ttttgtttgg ttgttcttat tatcgcagat gcaagctttt aaacaccatt tagtggagaa
     8941 gctgtgacag agatgttcag ccttgagcga taagaagcga ttttgaaaaa tcgcttccca
     9001 tattttacaa aatcgtgcct tattgccgat aggctatctc tggaacaccg tttattcaga
     9061 aaaaggagct gcgacaaggc ttttgtcaca actccgttca taaatatttc ctgtcaatcc
     9121 agcataatcg gttgaataaa atcggcttgc catctttttt ctttctcaga aaaatcaaaa
     9181 taccatatct gagaaaaata ctgtcgttgt gtcttacttc ctgagatcaa tcgcccatct
     9241 tttcgatcga ctgaataatc acagcatctg aaatcaattc ggatggcaaa actattttcg
     9301 tggatctgtc gataatttcc tacttcctga atagttattt ttttagtatg atttcttaca
     9361 ccgtctgctt tgtttttttg cagaactttc tgatgttcgg caaacagctt gtctgtataa
     9421 cagcttctgg ctttttcagg agtttcttct tcccaagcgg attggatctt catgaatatc
     9481 ttttggattt ccaaaaactg tgctttcttt tctttagccg ttcctgacat gcgggacaat
     9541 atctccgatt tcagcctttt cttttgtctt ttattcttga cgatagaaaa agcagaaacc
     9601 cctcctactg acaaaacagc taatgcaaga aaccccgttt gctcataact agaaggacct
     9661 gtacttggac cataataccc ataagaatct ctgccgtaag aacgactgcc aaaataactg
     9721 cccccatatg aacgaccgcc aaatgaacct ctgcccgaac ttcgaccgct agatgtacct
     9781 ccaccatgac cacccgcgac agcgaatcct tgggtcggca gagcaaacaa acataggccc
     9841 actaacaaaa tgattgctaa ataattgatt ttttcattgt cttcctcgtt atcttttatt
     9901 acaaaaaact attttatcat agccttgcta aaaggaagag tcaataacca gatgcataaa
     9961 ctgttttttt agcaattttg tcttatttag tcattgagta acatcggaca gatgtatgtg
    10021 tcggcagtcc ataattcctc tatcttcagc atgaacaagc ttgcagacaa gagatctgcg
    10081 cttcctccag gagagatttg gttttcctga caaaagtgca acagtgctgt aaatggacga
    10141 tttggtaaag cactttgact agcagcatga gcaagttttt gcatttccac taacgttttc
    10201 tgatcactgc gatgcaatac gcatgtgtca tctagcgaac tcatcaaaac taacaacata
    10261 tgcatttgtt tctgtacata tgaatcatta tctagctcct tagctgcttg gattgcaagc
    10321 ctgatatgcg gataccctaa tatcgcttct tcatatgcac catttacgcc atatttttgc
    10381 ttcaccctat gaccatgagt ggttttttta tgacgatatt ttgtatcagg aaaagcagca
    10441 agcttgctga catcatcaag tattttcgat aaagaaaaag gctgttccat actgattttt
    10501 tgtgcaatga cactgatcaa tagccccatt gcccaaatcg ctcctttatg ggtattgatg
    10561 ccatttgtag caatcagcat tgccttttcc gcttttcgcc caatctcagc aattctttct
    10621 cttaactcct gatcaatagc ataatgccat gctatttgag ccatttcttc aaaaaaagga
    10681 gttagcgtct tagcagactt atccaaaatg cgccgacctt cttttacggc ttttgcgatc
    10741 gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac ttcactacct
    10801 ttgattccca tagcgattcc tatatcggct cttttcaaag caggtgcatc atttacgccg
    10861 tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttcacgat tcgcagtttg
    10921 tgttctggtg tcgttcgagc aaagacgtcg acttttggta cttgtcgaat caactcttct
    10981 tcagacataa gatccaaatc gatcccttct aaaactttag cagtatgttt taagccaatt
    11041 tgttccgcga tcgcttttgc tgtatctttg tgatcccccg taatcatttt cactgagatt
    11101 cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg atcaatcaac
    11161 ccagcaatcc ctgcaaaagt taattgggtt aaagactcat gcgtgagttc ctgtttcgaa
    11221 tctacttttt tgtaagcaaa tcctaaaaca cgctgacctt ttttcgcaaa cgctgtggct
    11281 gtttgacccc atgcttctta ttctgaagaa gaaagagttg atttagccaa taatacttct
    11341 ggcgcacctt tgacgtaaat cacccctcct ttttcctccg catgcatcgt tgccatatat
    11401 ttgtattcag agctgaacgg aatttttgct tctatttttt tcaaaggcag atcggctgtt
    11461 tcggtgtggc gaatcagtgc aagttctgtc ggattgccac ttaaattttc tgttttctgt
    11521 tcttctttcg tctttaattc ctgacaattt ttcataataa acagtacgcg agtttcttcc
    11581 tttgctgcta catccacaac tgtcatttca ttttttgtca atgtccctgt cttatctgaa
    11641 cagatcactg tcatagaacc taatgtttct acagaaggca gtcctttgat gatcgccttt
    11701 tcttttgcca tttcatgtac tccgacagat aggatcatgg tcaacacagc tggcaatcct
    11761 tcgggtacca ttgacaccac caaagcgatc atagctgaaa gaaggatatg ccaatccatg
    11821 ccatagcgga atgttgtaaa aacaactaag aacaagatca aacaaagaat gccttgaaaa
    11881 atttgtttgt tcaatcggtt gatcttcttg atcagaggcg tcgtttgttg ttcgaccgat
    11941 tgcagtgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac aacgacaccg
    12001 actgcagaac cagattgtac aagcgttcct gaaaaagcca tatttttttg gtcaccgatt
    12061 gattcttctg ctgccaaaac ggaatgattt ttttcagatg cttctgattc tcccgtcaaa
    12121 atcgattctt ctactagtag atcatgtact tcgatcaacc gaacatctgc tggaacgacg
    12181 tctccggcac gtaagacaat gacatctcct tttactagtt ctttcgcagg gattttttct
    12241 tgcatcccgt tcgatagtac tatcgcttct tgtcccatca gctgcttcaa accattcaac
    12301 gattcttctg cttttctttc ttgccaataa ctaacgagtc cgttgacgat cactacgaat
    12361 aaaataatac ttccttcgat gtattcgctc gaagcaaatt tcaaaaaaga ggcaatgatc
    12421 aacacgatca tcagcagatc agtaaagtgt tttgctattt ttttccatcg ttttgtctgc
    12481 tgtcttgctt ctatctcatt atatccttga tcagtcaggc gtttctgccg ttcttcttgc
    12541 gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt caatctgtac
    12601 cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg gaagtttttt
    12661 caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcagaa tgtatctggg
    12721 cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat acaaaccaga
    12781 taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg
    12841 aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc cgtttctttt
    12901 ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga aaacaactcg
    12961 ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa aaatgtagac
    13021 aaagaccgac aaaaaataac atccctgtaa ttaaaatacc gtgtaaaggt acgcctttct
    13081 tagaaagcgc acaaaaccaa tggggcaaac ttttctcttt tgctgtttga tataaaatcc
    13141 tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat
    13201 tgacaattgc tgctccgctt tggcaatgga tcacttgcag catagtcact aaaggacttt
    13261 cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat
    13321 agaaaaagag gatacgccaa ggcagacagt tgattgcatt tttcaaattc ttttgtgggt
    13381 cactcgtctc accaactgtt aatccgatca tttcaattcc agcaaaactg aataaaacca
    13441 taggaaatga agcaatcatt ccagcaaagc caaacgggac taattcattg agctgtccaa
    13501 aatttgcttt gattccaata gactgggtat gaaaatctga gacactcaat cctataccca
    13561 ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac
    13621 caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc
    13681 ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt
    13741 ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact
    13801 ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa
    13861 ataaatcacc catgacccgc atcaaaccat aaatagccaa accgcatatc aaataaacga
    13921 gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc
    13981 caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg
    14041 ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc
    14101 caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg
    14161 gctgtgactg caattagatt gatccatgtc ccagctttca tcgtcagtcc gacagaaaat
    14221 ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct
    14281 ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact
    14341 gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc
    14401 aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg
    14461 acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg
    14521 aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag
    14581 gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca
    14641 ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc
    14701 aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg
    14761 aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggatttttc
    14821 aaccgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca
    14881 aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg
    14941 cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc
    15001 atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc
    15061 atatcaaatt tcaaattcaa aaaagtgatg atccaataaa cgactaaaat gacaactaat
    15121 gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagca
    15181 agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca
    15241 acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg
    15301 ggaaacattc cggcaaattc accagagatc aatgagattg gaaaagcgta caataatact
    15361 gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt
    15421 acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat
    15481 gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt
    15541 ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat
    15601 caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc
    15661 caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg
    15721 ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat
    15781 tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt
    15841 ggcgatttct tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat
    15901 agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat
    15961 tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga
    16021 agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt
    16081 ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt
    16141 gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt
    16201 aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat
    16261 caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc
    16321 ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt
    16381 tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg
    16441 tttgattacg attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt
    16501 gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac
    16561 gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa
    16621 ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg
    16681 taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga ttttatctac
    16741 actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat
    16801 tttttgttca gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc
    16861 ctcttgaact gggatagcaa ccatctgatc taagcctact ccgcaaatat ctaacgcctt
    16921 catccaagaa tagtgttttg tttgaggcac cagccatttc cctaatcttt ggatattttt
    16981 tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt
    17041 catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac
    17101 ttgttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcagc gggcgtacca
    17161 taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat
    17221 attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga
    17281 agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc
    17341 aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat
    17401 cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt
    17461 ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat
    17521 ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac
    17581 tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt
    17641 tcttccctcc atattttgtt ggtcaatgtc tcgcagatac atgtgtttat ctgtaacacc
    17701 tcttcactat agcgccaaaa atgagaaatt tttttttata acatcaaatc cacgtagatt
    17761 ttta
//
LOCUS       DAPBAB010000051        17740 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPBAB010000051 DAPBAB010000000
VERSION     DAPBAB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.053"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..1030)
                     /locus_tag="RD444_002068"
     CDS             complement(<1..1030)
                     /locus_tag="RD444_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7549432.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="RD444_002069"
     CDS             complement(1112..1801)
                     /locus_tag="RD444_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7549433.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="RD444_002070"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="RD444_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDT7549434.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="RD444_002071"
     CDS             complement(2328..2831)
                     /locus_tag="RD444_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDT7549435.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="RD444_002072"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="RD444_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDT7549436.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="RD444_002073"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="RD444_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDT7549437.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="RD444_002074"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="RD444_002074"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDT7549438.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="RD444_002075"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="RD444_002075"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDT7549439.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="RD444_002076"
     CDS             complement(6443..7462)
                     /locus_tag="RD444_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDT7549440.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="RD444_002077"
     CDS             complement(7635..8540)
                     /locus_tag="RD444_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7549441.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="RD444_002078"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="RD444_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDT7549442.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="RD444_002079"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="RD444_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDT7549443.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="RD444_002080"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="RD444_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7549444.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="RD444_002081"
     CDS             9753..10397
                     /locus_tag="RD444_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7549445.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="RD444_002082"
     CDS             complement(10453..10956)
                     /locus_tag="RD444_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDT7549446.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="RD444_002083"
     CDS             11553..12518
                     /locus_tag="RD444_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDT7549447.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="RD444_002084"
     CDS             complement(12617..13789)
                     /locus_tag="RD444_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549448.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="RD444_002085"
     CDS             complement(13776..14921)
                     /locus_tag="RD444_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549449.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="RD444_002086"
     CDS             complement(14935..15867)
                     /locus_tag="RD444_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549450.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="RD444_002087"
     CDS             15984..17063
                     /locus_tag="RD444_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7549451.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="RD444_002088"
     CDS             17060..17689
                     /locus_tag="RD444_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7549452.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
BASE COUNT         5331 a         3698 c         3192 g         5519 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg
//
LOCUS       DAPBAB010000052        17538 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPBAB010000052 DAPBAB010000000
VERSION     DAPBAB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.054"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(37..846)
                     /locus_tag="RD444_002089"
     CDS             complement(37..846)
                     /locus_tag="RD444_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7549453.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1139..2575
                     /locus_tag="RD444_002090"
     CDS             1139..2575
                     /locus_tag="RD444_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDT7549454.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2745..3941
                     /locus_tag="RD444_002091"
     CDS             2745..3941
                     /locus_tag="RD444_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7549455.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4003..4188)
                     /locus_tag="RD444_002092"
     CDS             complement(4003..4188)
                     /locus_tag="RD444_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDT7549456.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4419..7733
                     /gene="dnaE"
                     /locus_tag="RD444_002093"
     CDS             4419..7733
                     /gene="dnaE"
                     /locus_tag="RD444_002093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDT7549457.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7901..8863
                     /gene="pfkA"
                     /locus_tag="RD444_002094"
     CDS             7901..8863
                     /gene="pfkA"
                     /locus_tag="RD444_002094"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDT7549458.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8937..10721
                     /gene="pyk"
                     /locus_tag="RD444_002095"
     CDS             8937..10721
                     /gene="pyk"
                     /locus_tag="RD444_002095"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDT7549459.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10756..11658)
                     /locus_tag="RD444_002096"
     CDS             complement(10756..11658)
                     /locus_tag="RD444_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7549460.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11844..12413
                     /locus_tag="RD444_002097"
     CDS             11844..12413
                     /locus_tag="RD444_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDT7549461.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12471..12650
                     /gene="rpmF"
                     /locus_tag="RD444_002098"
     CDS             12471..12650
                     /gene="rpmF"
                     /locus_tag="RD444_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDT7549462.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12820..14241
                     /gene="gndA"
                     /locus_tag="RD444_002099"
     CDS             12820..14241
                     /gene="gndA"
                     /locus_tag="RD444_002099"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDT7549463.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14434..15120
                     /locus_tag="RD444_002100"
     CDS             14434..15120
                     /locus_tag="RD444_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7549464.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15117..16622
                     /locus_tag="RD444_002101"
     CDS             15117..16622
                     /locus_tag="RD444_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7549465.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            17309..>17538
                     /locus_tag="RD444_002102"
     rRNA            17309..>17538
                     /locus_tag="RD444_002102"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5673 a         3059 c         3681 g         5125 t
ORIGIN      
        1 tgtcacaacc tcttttttgt ggaatctaat tctctattat agtttaagta gtttttccag
       61 ataaacagct aaaccgtctt cctgattgct caaaggagtg acatcatttg caatgccttt
      121 gagttggtca gtaccgttag ccatagctac tccccaacct gcataatcca acaattccaa
      181 atcattatgc tcgtcaccaa aggcaatgac gtctttttga tcgatagaca ggtagttagc
      241 aatctcttgc acgccttttg ctttttggat tccttttgaa acaatctcta agatggcagt
      301 aggtccaccc catgtccgaa catcgacata actgccaaat tgacgtgtta attcagcaga
      361 tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa gatgtaggat ttgtcgtcaa
      421 atttttagga gaaagcaaat tcttctctcc tggacgagaa gaagcaaaaa ttttttcatc
      481 aaagaaatca aaactgtcaa taaaaaaagt atcacgattt tcagcagcga taaaatctaa
      541 atttagctgc tttttttgcg ataagatttc aaaagcgatt tcacgattga tcaacgtttc
      601 tctttcacca tcccagtgct gattagggat gtgtactaat gcgccgttaa aattaaccat
      661 aggtgttgtt aattctaatt gttggtaaaa ctgatggctc attcggaatg ggcgtcctgt
      721 agcaatactg acatgatgac cggcattgat tgcttttttt agagtctctt ttgttttaga
      781 tgtaatcatc gaatcttgat tgagggtagt tccgtccaga tcgatggcaa ttaatttttt
      841 attcaataag ggtccttcct tcctcaacta gcatttgttt tagtctagca tattttaggt
      901 aattaacaag agcaatcagc tattcctaac aaaaactaca gttatggcta tataaatagc
      961 taaacataag ctaaacacga acgttaagta ttattttcgc tttttttgtt aaaaaaacgt
     1021 tgaaaagcga atgaaacgtc tatataattt gaaattgttc gatggatttg ccctatttta
     1081 cttatttttt attattatag ttttccatca gaaaaaaatt cagggagtgg aactttttat
     1141 ggaaaagttt ttcggtttaa agaaaaacaa tacaaatgta tcaactgaaa taatggcggg
     1201 agtaacgaca ttctttgcca tgagttatat cttattcgta aatccgacga ttttgtcagc
     1261 atcaggtatg cccttccaag cagtatttct agcgacaatc attgcatcga ttattggtac
     1321 gctggtaatg ggattgttcg caaatgtacc atatgctcag gcacctggta tgggattgaa
     1381 cgcatttttc acttttacgg tcgtttttgg gcttggttat tcttggcaac aagccttggc
     1441 aatggtgttt atttgcggat taatcaatat ttttattact gtcacaaata ttcgaaaaat
     1501 gattattcgg gcgattcccg aaagtcttca acatgcgatt ggtggcggga tcggtatttt
     1561 cgttgcgtat gtcggaatca aaaatgcagg attcttatct ttttcagcag accagtcagc
     1621 tatttcgagc tctgtcgttg aaggcggaaa agcgacaaat gttacgataa atggtggaat
     1681 cgtccctgcg ttagccaact ttgacaacgc tcctatctta ttggctgtga ttggtttggt
     1741 actgacagcc attttagttg taaaaaatgt ccgtggggct attcttatcg gtatcgtagc
     1801 gaccacagtc cttggtattt ttatgggtgt tgttgattta agttccattg attggcaaac
     1861 gaattcgtta ggtaattcat ttaaagaatt aggaacaact ttcggtgcag catttggttc
     1921 tgaaggcatg caatctttat tcagtgattc ttccaagatt cctcaagtat tgatgacgat
     1981 catcgcattc agtttatctg atactttcga tacgatcgga acatttatcg gtacaggacg
     2041 ccgcacaggt attttctcaa aagaagacga gatcgcgttg gaagatggtc gtgggttcaa
     2101 aacaaaaatg gataaagcac tatttgccga tgcgatcgct acttctgtgg gggctatctt
     2161 tggtacatcg aatacaacaa cttatgtcga atctgcagct gggatcgggg ccggtggacg
     2221 tactggtttg acttcagtag tagtggcagt gctattcgca ttaagtagtt tattctctcc
     2281 actgatcgcc attgtaccaa atcaagcaac tgcgccagca ttgattttag ttggagtaat
     2341 gatgatggct tcttttgcag acatcaaatg gctagatatg gaagaagctt taccggcctt
     2401 ctttgcatcg atctttatgg gattgtgcta tagcatttcc tatgggattg cagcaggatt
     2461 tattttctat acaattgtga aagtaatcaa aggaaaatac aatcaagttt ctgttgcttt
     2521 gtggatcgtt gatattttat tcattatcaa cttcatcatt ctagcaacaa tttaactaaa
     2581 gagaagggat tggaacagta tttgttctga tctctttttg ttttacataa aaagaacgtt
     2641 acatataaaa tgacctttgc gggatttttc tctttttact ggcgagatcg ttgcttgttt
     2701 attcgaaaat aggtatgata aaagagacga aagagggatg atagatggag attaattgga
     2761 gaaaaaattt agtgattgcg tggattggct gttttttcac tggtgcaagt attagtttag
     2821 ttatgccatt cataccagtt tatgttgagc aactaggaac accaaaagat caaatcgaat
     2881 tgttttcggg actagcaatc tctgtgactg cttttgcatc tgccgtggtt gcaccaatct
     2941 ggggaaatct agctgatcga aaaggccgaa aactcatgat gatccgtgca gcagcaggga
     3001 tgacattaac aatgggatct ttggcttttg taccaaacgc gtattggctt ttgatcatgc
     3061 ggttttggaa tgggattttg tctggatata ttccaaatgc cacagcgatg attgcttcac
     3121 aggcacctag agaaaagagc ggatgggcgt taggaacatt gtctaccgga gcgattgctg
     3181 gaaatctgat cggaccttca atgggcggtg cactggctca gtggtttggt atggaaaatg
     3241 tattcttgat cacaggggct ttgttgatga tcacaacggt attgaccatt ttcttagtca
     3301 aagaagattt ccatccaatc gagaaaaaag atctgataag caccaaagaa attttttcaa
     3361 aaatggatca tctctctatt ttgatcggtc tcttcattac gaccttgatt ttacagatag
     3421 gaatcactac gatcagccct attttgacct tatatattcg tgaattgagc ggaagtacag
     3481 aaaatatcct ttttgtcagc ggcttgattg tttcgatagc tggtgtttcc gctgtctttt
     3541 cttcaccgaa actaggaaaa ctaggggata agattgggaa tcaaaaagtt ctaattggag
     3601 gactcatttt atcattttgc tgttatttac ctatggcatt cgtcactaca ccgctccaat
     3661 taggtatact tcgctttatt ttaggctttt caactggcgc tttgatgcct tctgtcaata
     3721 cactgatcag taagattacg cctcaagaag gagtcagccg aatctatagc tacaatcaga
     3781 tgtttagcaa ctttggacaa gttttaggtc ccatgctagg atcgacagtc gcgcatgctt
     3841 ataattacag tgctgttttt attgtgacaa gtttgtttgt tctgtcaaat attcttcttt
     3901 ctttattcaa ctttcgtaaa gttcttcgca gcagattata atggtggtga ggagtagaaa
     3961 ataaaaaaga gcttgagaaa tctcaagctc ttggctgttt ttttacgaat gggcagaggt
     4021 ttcttttctt ggcgcaactg caccaatcaa aaggataatg acacccgcaa tgaatgaaat
     4081 gattgttgtc aaagtaaaat catatgttcc gcttgttaat gcagcaccaa tataaccgac
     4141 tacttgacct agaacgacag cccagaataa tgtaacgata tatcgcataa agaacacctc
     4201 ttttctatct taattttacc agattttctt tgaatgtaaa gaaacgagac caaatctcaa
     4261 taaaatttga gttcttgcga aagaactcat tcctacagat tacaagcaag caaatgaagt
     4321 ggaataatca acttcagcgc ctgctatctt aatgaaagta aaaggatcaa accttttaac
     4381 gctagtgaat tttaacgaaa aaaaaggcgg aatgcaagat gtttttccca caactttata
     4441 caattacttc ttattctctt ttacaaagta cgatacgtat tcgagaatat gtacaagaag
     4501 caaaaaaaca aggctacact tcattagcca taacggataa agatgtctta tacggtgcac
     4561 ctgaattcta tcgtatttgt gtagaagaag gagtacagcc aatcattgga ttacatctta
     4621 gttatactta tgaagaaaaa aattatggct tgcttgcgta cgcaaaagat gaaataggtt
     4681 atcagcagct gatgaaactt tccagccgaa aaatgatcca agaaaaattg gaacttgcag
     4741 aaatgacaga aaccgaccat ctagcgtttg ttgttccggc aaatcattct gtttctgagg
     4801 ctttcaataa agaggataac tttttcggtc attggcagtt tttacttgaa aatgttttta
     4861 ctagaaattt ttatatcggc ttgacagaaa acacgcctga tcaattgcct gaatggtatc
     4921 agtatgtgat aaaagaaaat tattcgcttt gtgcattaca tcccatcgct agtttacaac
     4981 aagaagaact ttttgcactt gaagtgatga aacatatcaa agagggaagc caaatacctt
     5041 tagaaaattt atctgcatct ttaccactaa cagcaaatgt ctatcttctt tcagaacaag
     5101 aaatgatcaa acgattcgaa aaagcgggtc tttcccaagc tgtaataggc gcccagcgtt
     5161 tagctgaatc gatttcattt gaattccagt tccatcagaa attacttccg cattatccag
     5221 ttcctaatgg aaaacaagct ggagaatatt taagagagtt atgctattcg caagtggaac
     5281 gccgaatcac tgactataca aaggaatacc aagaacgttt ggactacgag ttatctgtca
     5341 tccatcaaat gggattcgat gattatttct tgatcgtatg ggatgtaatg gcttttgctc
     5401 atagaaacaa aatcgttacc ggtgcaggtc gtggttctgc tgcgggttca ttagtagcct
     5461 atgtgttaga aatcacagat gtggatccat tagaatatga tttgctgttc gaacgctttt
     5521 taaaccccga acgctttacg atgccagata tcgatttgga tattcctgac aatcgacgtg
     5581 aagaagtatt gaattatgtt cgagaaaagt atggacaata tcatatggca caaatcgcaa
     5641 catttggcac aatggcagca aaaatggttc ttagagatgc agctagagtg tttggtttat
     5701 cgcaaagtga agcaaaccgc tggtcaaaag caatacccaa tcaattgaaa atcacattga
     5761 cccaagctta taaaacttcg catcaattag tagaattagt tcaggcaagc gaaaaaaacc
     5821 aacttttatt caaaactgca gtgattttag aaggattgcc aagacatgtt tcgactcacg
     5881 cagcaggtgt agttatcagc gataagaatc tacttgaact agttccgctc caacgcggtt
     5941 ctgatgaagc ttttctgacc cagtatacga tgaatgaggt agaagcaatc ggtcttttga
     6001 aaatggactt tttaggacta cgcaacttat cgattattga ttttactttg aaagctgtta
     6061 agcgagtaga agggtatgag atccgattaa aagagatccc gctaaatgat gcaaaaacgc
     6121 tgctcttgtt tcaacgaggc gaaacctcag gagtatttca atttgaatct gctggtatcc
     6181 ggaacgtttt acgaagatta ggtccagaaa acatcgaaga tattgccgca gtcaatgcat
     6241 tgtatcgacc aggccctatg caaaatatag ataccttcat tgccaggaag aaaggaaaag
     6301 aagccattcg ctacccagat gattcactga tcccgatttt gaaaaacaca tatggagtga
     6361 tcgtctatca ggaacaaatc atgcagatcg cctcgactat ggctggattt acattagggc
     6421 agtcagatat tctgcgacgg gcagtgagta aaaagaaaaa agacgtatta gatgaagaaa
     6481 agtcgcattt tgtatcagga gcattacaac agggatattc ggaaaaaaca gcagcgaccg
     6541 tatatgaata tatcgaaaaa tttgcaaact atggattcaa tcggtcacat gcatttgctt
     6601 attcattcgt gggattccaa atggcgtatc taaaagccca tcatccaggt gctttcttta
     6661 ccagcctgat gaattctgtt cggcataaca catcaaaatt gaaagaatat attgcagaag
     6721 cacgtaaaaa taaactcaaa ctttcctcgc cgtccattaa tcaaagtaat tccagttttg
     6781 agttggtcaa taaggaagaa atcagattcg gactgacagc gataaaaggt attcggcgtg
     6841 attttgtaga agatatatta aacgaaagaa aacaaaatgg cccgttccag tcagtggatc
     6901 aattcctcat acggatcgat aagcgttggc tgaaattaga gttactgcaa tcactgacag
     6961 cagtaggcgt ttttgatgaa ttatctccta acaggaaaca attgatgctt gatctcgaag
     7021 gaaaaatcca aaatatcgtt tacagtggtg gaagtcttga cttgctggga atcatggcat
     7081 taaaagaaga agaagtcgct gactatacac tagaagaacg cttgaagctg gaagaggagt
     7141 acttaggtat ttatttgtca ggtcatcctg cagaagggtt tgatcgtctt cgtttagcta
     7201 aacaagttca gctgattaca gatatcgtcc ctaatcaacc agtcaattta ttagtgatga
     7261 tcaaggaagt tagagaaatt cggacaaaaa aaggagaact gatggccttt gttgaaggaa
     7321 ccgacagtag tggagaaatt tctgtcacag tttttccaac tctttatcgt aaatatcgta
     7381 atttgtttgc cgttgatcga gtgatctttg ttcaagggcg gagcgaaatc agcaaatata
     7441 atcaggaact gcaactaatt ggagaaagct ttgctgatcc taaccagtta gaagcccagt
     7501 atccttcaca aacttgctat ttgcgtatta cagaagaacg ggaagatccc gagataatga
     7561 aacaattaca agtgattttt cagaaacacc caggatatat accagtagtt atgtatcacg
     7621 aaaaaacgca acgaaaagta gttctgtcag atgcttattg ggtggatgac agcaagagta
     7681 taaaggggca acttatttat ctgcttggcg aagataatgt ggttttcaaa taacttacgg
     7741 taaaaaatac tacttgcttt catttttttt cattttttta tccgtaaatc aagacaagtg
     7801 agaaaaataa tgttagaatg ttagtcgtgg ttaacgcaaa aatggttgta aacctaagtc
     7861 atacttagga taaagtattt taatgaggtg aatgtttatt atgaaacgca tcggaatttt
     7921 gaccagtggc ggagacgcac ctggaatgaa tgcagctgtt cgtgccgttg ttcgtaaagg
     7981 tatttacgaa ggactggaag tatacgggat caattatggt tttgccggtt tagttgctgg
     8041 agatattcgt cgtcttgatg tggcagatgt aggtgacaag atccaacgtg gtggtacatt
     8101 cttgtattct gcacgttatc cagaatttgc aactgaagaa ggtcaattaa aaggaatcga
     8161 acaattgaaa aaattcggaa tcgaaggtct agtggttatc ggaggagacg gctcttatca
     8221 tggcgcaatg gctttgacaa aacgcggttt cccagcagta ggtattcctg gaacaatcga
     8281 taatgatatt cctgggactg atttcacaat cggttttgat acagcaatca atacagtact
     8341 agaatcaatt gaccgtattc gtgacacagc gacttctcat gttcgtacat tcgttatcga
     8401 agtaatggga cgcaatgcag gagacatcgc tttatggtca ggtgtggctg gcggagcgga
     8461 tgaaatcatc atcccagaac atgattttga tatggcttca gttgctaaaa aaatccaaga
     8521 aggccgcgat cgcgggaaga aacattgctt gatcatcttg gctgaaggtg taatgggcgg
     8581 aaatgaattc gctgagaaac tttcagaatt tggcgactat catacacgtg tatctatctt
     8641 gggacacgtt gtccgtggtg gagcaccaag cgcacgcgac cgcgtgttgg caagtaaatt
     8701 cggtgcttac gctgtcgaat tgcttcaaga aggaaaaggt ggattatgtg tcggtatcca
     8761 tgacaacgaa atcgttgcgt ctgacatcat tgatacactt gaaaacaaca aacataaacc
     8821 agacttatca ctatatgatc taaataattc actttcattt taagagtgaa agaaaccaag
     8881 ctatttaagc tgtgtacagc tttaaataaa caaaattgaa tgggagagtt ttttaaatga
     8941 aaaaaaccaa aatcgttagt acattaggac cagcaagtaa ctcagtagaa attatttctc
     9001 aattgattga atctggggca aatgtcttcc gctttaactt ttctcatggt gaccatgaag
     9061 aacaattatc acgtatgact atggtccgtg aagcagttaa aaagacagga aaagatgtcg
     9121 gtattctttt agatacaaaa ggagctgaaa tccgtacaac tgttcaaggc acaacagaag
     9181 ctgattttgg tcgtgcagga tacatcaaat ttgaagtagg agatcaaaca cgcatctcta
     9241 tggatcctga acatgtaggt tcaaaagaaa aaatcgcggt tacttaccca ggattatttg
     9301 acgatgttca cgttggcgga cacattttat ttgatgatgg tttgatcgat atgcaaatca
     9361 ttgaaaaaga cgaagcaaac cgtgaattag tcgtagaagt taaaaatgca ggtatgctag
     9421 gatcacgtaa aggggtaaat gcaccaggcg tttctattag tttgccaggt attactccaa
     9481 aagatgctga cgatattcgt tttggtttag acaacgatat cgactttatc gctgcaagtt
     9541 ttgtacgtaa agcacaagac gttctagata tccgcgaaat cttagaagaa aaagacatga
     9601 ctcatgttca aatcttccct aaaatcgaat ctcaagaagg tatcgacaac attgacgaaa
     9661 tcatcaaagt ttctgacggt gttatgattg cccgcggaga tatgggtgtt gaaatcccag
     9721 ctgaactagt accaatggtt caaaaacgca tcatcaaaaa atgtaatgct gctggtatcc
     9781 cagttatcac tgctacacaa atgttagaat caatgcaaga aaacccacgt ccaacacgtg
     9841 cggaagcttc tgacgttgct aacgctgtat ttgatggcac agatgcaaca atgctatctg
     9901 gtgaatctgc aaacggggac tacccagttg aagctgtttc aacaatggca cgtatcgacc
     9961 aagaagctga aaatgcatta tcagaattag gtacattcca attgaacgaa ttcgataaaa
    10021 cagatgtaac tgaaacaatc ggtttatctg tagctcgtgc tgcaaaaaat ctaggcgtga
    10081 aaacaatcgt tgctgcaact gaatcaggat acactgcaaa aatgatttca aaatatcgtc
    10141 cagatgcaga tatcttggct gttacttttg acgaacgcac aaaacgtggt ttgatgttaa
    10201 actggggcgt ttacccaaca gttgctgaaa aaccatcaac aactgatgaa atgtttgaac
    10261 tagcagctaa aaaagctgtt gaattaggtt tcgcatcaga aggcgattta atcttgatca
    10321 ctgctggtgt accagttggt gaacgcggaa caactaacgt gatgaaaatt caaatgattg
    10381 gttctaaatt attagaagct caaggcgttg gacaaaaatc tgtcgttgca aatgcagtta
    10441 ttgcttcatc tgctgaagaa gctgtaaaaa aagcaaaaga cggaatggtt ctagttgtac
    10501 catcaactga caaagaatat atgcctgcaa tcgaaaaagc agctgctgtt atcgttgaag
    10561 aaggcggatt gacttctcac gctgcagttg taggaattgc acaagatatt cctgtaatcg
    10621 ttggtgctaa aggcgcaaca acaacaatta cagaaggtga attgattacc gttgattcac
    10681 gtcgcggcat cgtttaccgt ggcgaaacaa cagctatcta atttagctta ataaaaaata
    10741 aaaacagctc ttcttttacg aagagctgtt tttttgtgct gtaacgaatt catggataac
    10801 ttggactact tgttcttgaa gaggcgtgac gatctgctgt gttcgttttg ccaatgaaat
    10861 caagaagcga ggctgaactt catctgaaag agggaggcaa actaaatgat cttcaggatg
    10921 gacagcaatc tctgccaaaa aaccgatacc cacattttct cggaccatac tctttaaaat
    10981 cgtcaagtca ttgctttgat aaacaacttt tggatgagta tgtgtttgtt tagaaagctt
    11041 agtgaatgct gcggggtgga cataatgttc gttcaatagt acaaactgtt catctgctat
    11101 atctttgaaa gagacagtat cctgcttgga aagaggatgt gtaggtggca cgacgatcat
    11161 gaaacgtttg tccaaaagta actctgtttc taaatattga tccacgattg gctgtgttga
    11221 gcctaataca gctaaatcga tcctcccttg tcggatcatc ccgtataagt cccgagagcc
    11281 tccatcggta atctcaattt gattcatcaa ttcatgcttg ataaagtaac ttgacagttt
    11341 aggaaagtat tcattgccga taataggagg catgccaaaa cgaagctttt tcttttgcaa
    11401 ctgagtgatt tcagctcttg cagtagaaat ctcttggaga atggtttgtg catggagaaa
    11461 cagctgcttt ccagaaggcg tgacgattaa ttcgtgatgc gatcgatctc ttgtaataag
    11521 tacggtgtca aactcttttt caagacgttg taatgcatat gtaatagtag gttggctcac
    11581 acggaaataa ttagccactt tcgtaaaatt tttttgtttg acgatttgga catagtattc
    11641 gatatctttc gtgttcatga agatcatccg ctcctttaat ggaataatga ttattgtaaa
    11701 tgattctgag gatgatagca aacagaggct ttctaataaa gatttttctg tttttttatg
    11761 aactgcaagg tgtcaagaaa atttcattga caacatgctt tttgccctgt ataattgctt
    11821 ttgttgctta ggagtgatga aaaatgaaat ggtctttatt ggaattgaga aaataccagg
    11881 agacaccgtt aacttttcat gagacgctcg acttgaaagc cgctctcatg aagcgagaca
    11941 atttaatact tgatgttgcg ccagtggaag tcgaaggttt ggtctcagtt gataagaaag
    12001 gctatatcgt tcattacact gtgcagacga ctttgacagt tccttcaaca cgttcactag
    12061 aacctgttga actgcccatg gatttttctg tagacgaagt attcatgaca gaagaacaat
    12121 tccaaagtag agatgaacaa atgtctgcgg atgagatttt gctaattgaa ggtccattat
    12181 tgaatttagc agagtcgatt gaagacaaca tcttattggc gattccaatg cgggtattga
    12241 caaaagaaga ggaagcagca acagaacttc caaaaggcaa tgattgggaa gttgtttccg
    12301 aagaagaata ccagcgcaga aaaatggaag aagccgagaa taaaattgat cctcgtcttg
    12361 caaaactatc tgaacttttt gattctaaat caaaagacga cgataacaag taagattgga
    12421 atatattgtg tctaatttag atacaagact ttacaaggag gtgtagcaaa atggcagtac
    12481 cagctagaag aacatcaaaa gctaaaaaag caaaacgtcg tacacactac aaattgacaa
    12541 tcaaagggtt aaacgaatgt ccaaactgtg gtgaaatgaa aaaaagccat cacgtatgtg
    12601 caaattgtgg ttactatgat ggtaaggacg taatgtcaaa agaagcttaa tacattttaa
    12661 ttagaaaatg taaaacctca aggctaacca tggagccttg aggtttattt ttttgtagtc
    12721 ggtaaataaa aaatatgttg ttctttagtt tgcaatgtag ccgtgttaag ctataatgta
    12781 agagattagg gagaaatccc gtatgtagga ggagttcata tgtcaaaaca acaaatcggc
    12841 gttgtcggta tggccgttat gggtaaaaat ctagccttga acatagaaag ccgcggctat
    12901 tcagtggctt tattcaatcg tacaggggct aaaacaaccg ctgtagtaga agaacaccca
    12961 gataaaaatt tcaaagcaac atatacaatc gaagaatttg tagaatctat cgagaaacca
    13021 cgtcggatct tgctaatggt caaagcagga ccggcaacag atgcaacgat ccaagagttg
    13081 cttcctcacc ttgataaagg cgatattttg attgacggag gaaatacatt ctttaaagat
    13141 acgatgcgtc gtaatgaaga gttagccaac tctggcatca actttatcgg tacaggcgtt
    13201 tccggtggtg aagaaggagc tttaaaagga ccttcaatca tgcctggtgg acaaaaagaa
    13261 gcctatgcgc tagttgcacc aatcttagag caaatttctg caaaagcaga agacgaagca
    13321 ccatgtgtca catatattgg cccaaatgga gccggacatt atgtgaaaat ggtacacaac
    13381 ggtattgagt atggagatat gcaactgatt gctgaatctt atgacttaat gaagaatatt
    13441 ttaaaccttt cagtagaaga aatggctgat atctttaaag aatggaatca aggcgaatta
    13501 gacagctatt tgatagaaat cactgctgac attctgacac gtaaagacga tgaaggaaca
    13561 ggtaaaccag tagtagatgt gatccttgat gcagctggga ataaaggaac aggtaaatgg
    13621 acaagccaaa gcgcgcttga cttaggtgtg ccacttccat tgatcactga atctgtattt
    13681 gcccgctata tttctgctta taaagaagaa cgcgtacaag caagcaagat tttatctcgt
    13741 acgaacgatt ttgagttcac gggagacaaa aaagaattgg tcgaaaaaat ccgagaagcg
    13801 ttgtatttca gtaaaatcat gagttatgcc caaggatttg ctcaattacg tgtcgcttca
    13861 aaagaatttg attgggactt gccatttggt gaaatcgcaa aaatctggcg cgctggatgt
    13921 atcatccgag ctcgattctt gcaaaaaatt acggatgcct atgacaaaaa tcctgagatc
    13981 gaaaatttat tgctagatga ttacttcgtt gaaatcacga aaaaatatca gcaatcagtc
    14041 agagacgtcg ttgccttggc tgtccaagct ggcgtacctg taccgacttt ctcatcagcc
    14101 atcgcttact tcgattcata cagagcagaa cgtctgcctg caaatataat ccaagcccaa
    14161 cgcgattact ttggcgcaca tacctatgaa cgtgtcgaca aagaaggaat cttccattac
    14221 tcatggtacc atgaagaata atagtctttc ggctgttagg aaatgagaaa ataaaaagag
    14281 gacgaaagag ctgaagtcga ttgttatgag tagacttcag ctctttttta aaattgctat
    14341 ccatgataaa atgactatag atttttaagc atattatcgg tataatgata gagtttttaa
    14401 aaagccagta gaatagaaag gataaaataa taaatgagca atattctaat cattgaagat
    14461 gaaaagaact tggcacgatt tgtcgagcta gagttgaagc atgaagggta taacgcagaa
    14521 gtccactaca acggccgaac tggtttagat gcggcattaa ataatgagtg ggatgccata
    14581 ttgttggatc tgatgctccc agaattgaac ggtttagaag tctgcagacg tgtacgccaa
    14641 gtcaaaaata caccaatcat tatgatgact gcaagagatt ctgtgatcga tcgtgtatct
    14701 ggacttgacc acggagcaga tgactacatc gtcaaaccat ttgctatcga agaattatta
    14761 gcacgtcttc gtgcattgtt gcgtcgaatc gatattgaag gcgataaaaa tgtggctaaa
    14821 caaactacta ttacttatcg cgatttgact atcgaaaaag aaaaccgtgt cgtacgtcgc
    14881 ggaaatgaag taattgaact aacaaaaaga gaatacgagt tgctgcttac tttaatggaa
    14941 aacgtgaacg tggtattagc acgtgacgtt ttactaaata aagtgtgggg atacgaaaca
    15001 gaagttgaaa caaacgtagt ggacgtatat attcgttatc ttcgcaacaa aattgacgtt
    15061 cccggggagg aaagctatat ccaaactgta cgcggaacag gttatgtgat gcgctcgtga
    15121 taaagccgaa aaaatcggaa aaaagagaac tgaaagggcc ctccttgaca atcaagtggg
    15181 cctttgcgag ttctttcttc atattcgttg tgtttacaat tttcgctgtc attacctata
    15241 aatcatctat caatctgatt gtggcaaaag aaagaaacaa tgtagagcgt acgatttcag
    15301 aagtagcttc tcgtttggct aactcggatg aagaactgac attagtaaat acgtatcgta
    15361 atctgacgaa agcgacaaat gacggagatg aggtcgtaga tcatacttca ctggtcgaag
    15421 gaaacttaat gaagatcgat tcctttatag ctgagctagg gcagccatca atggatcttt
    15481 atgtttatga tttggacgag aacttgattt ttaaaaccca tgaaaacagt cgaaacctga
    15541 ttcagactac acgtaaagtt ccaacgatcg ttacactaga aggaaaaact ggctttttat
    15601 ctatccagcc tatatattct aaaggtacaa gagaaaagat cggctatgcg caaagcttct
    15661 atgaattatc ttctttctat gatattcgaa ataagctatt attgactctt attgttttag
    15721 aagttgtttc tttgatctta agtagtattt taggtttctt tttatcttct tatttcttaa
    15781 aaccactcaa ggtactcaga gatacaatgg atactatacg taaagatccg caatcagata
    15841 tccacatgcc tgaaatcgac acaaatgatg aattggcaga tttagctgaa atattcaatg
    15901 agatgcttga ccgtatgcgt ttgtatattg aacaacaaga gcaattcgtg gaagacgtat
    15961 ctcatgagtt aaggacacct gttgcaatta ttgaagggca tcttagtctg ctaaatcgtt
    16021 gggggaaaga tgatcctgag atattagaag aatcattaag cgccagcatg caagaaatta
    16081 gccgtatgaa aagcttagtt caagagatgc tagacttatc aagagcagaa caagtagatg
    16141 ttcattatgc gaatgaaaca actaaaggaa aagaagtcac ctatcaagtt tataataatt
    16201 tcaagatatt atatccagac tatgtgatca ctttagatga tgatttgaca aaagaagtga
    16261 ccctgcaaat ttatcgtaat cactttgaac aattaatcat tatcatattg gataacgcag
    16321 taaagtattc aactacacga aaagaagtcc atatctctat ttcttccact ctgaatgaat
    16381 ttgagatcgc gattcaggac tttggcgaag gaatacccaa agaagacctt aataagatct
    16441 tcaatcgttt ttatcgagtg gataaagcaa gagctagaac gaagggtgga aatggtctgg
    16501 ggttgtctat cgctaaacaa ctggttgaaa actataaagg acgtatctta gcagaaagtg
    16561 tagtagggca agggacaatt tttagaatct ttattcctat cgcaaaggca gaagacaagt
    16621 aaagcatgtg ctttacttgt ttttttatat atccaacaaa gaataaggta caaaatacaa
    16681 gcaagttaga aattcaggga tgtcattata aaaaaaacta gcgaacaatt aaatagaata
    16741 acatccgtta attaaacgta agtagctttt agatgaacga aaaaacaatg aaatgctgta
    16801 atattcgtgt ttaaaaataa taaataaaaa tagggaattt tctattgcaa ttttataaag
    16861 agattgctat attattattt gttctaaatg attcctttaa ggtgtcacta aaaattataa
    16921 aaaatagggt tgacgcactt ttgatgtcat gttataataa cgaagtcgtt tggatgaggt
    16981 agctaataaa gcttgatttt atcgaaaaaa acttcgaaaa agttgttgac aaataacaat
    17041 aaagatgata tgataatcaa gtcgctgcta atgagtgaca aagacgttta gaaaaaataa
    17101 acaaagaagt tgttgacatt ctcttctgaa tctgataaga ttaaagagtt gttgaaacaa
    17161 aaagattaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
    17221 attcaattca agacaacaaa catttttaac aagcaagcaa tatgctagca aacaaattga
    17281 gcttaacaat cgcaagattg ttcgaacttt ttatgagagt ttgatcctgg ctcaggacga
    17341 acgctggcgg cgtgcctaat acatgcaagt cgaacgcttc tttttccacc ggagcttgct
    17401 ccaccggaaa aagaggagtg gcgaacgggt gagtaacacg tgggtaacct gcccatcaga
    17461 aggggataac acttggaaac aggtgctaat accgtataac aatcaaaacc gcatggtttt
    17521 gatttgaaag gcgctttc
//
LOCUS       DAPBAB010000053        17006 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPBAB010000053 DAPBAB010000000
VERSION     DAPBAB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.055"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..127)
                     /locus_tag="RD444_002103"
     CDS             complement(<1..127)
                     /locus_tag="RD444_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDT7549466.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="RD444_002104"
     CDS             complement(231..542)
                     /locus_tag="RD444_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7549467.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="RD444_002105"
     CDS             complement(544..741)
                     /locus_tag="RD444_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDT7549468.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="RD444_002106"
     CDS             complement(1135..2862)
                     /locus_tag="RD444_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDT7549469.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="RD444_002107"
     CDS             complement(2855..4048)
                     /locus_tag="RD444_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7549470.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="RD444_002108"
     CDS             complement(4235..4879)
                     /locus_tag="RD444_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7549471.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="RD444_002109"
     CDS             complement(4973..5107)
                     /locus_tag="RD444_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDT7549472.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="RD444_002110"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="RD444_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDT7549473.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="RD444_002111"
     CDS             complement(7238..7711)
                     /locus_tag="RD444_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDT7549474.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="RD444_002112"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="RD444_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDT7549475.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="RD444_002113"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="RD444_002113"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDT7549476.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="RD444_002114"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="RD444_002114"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDT7549477.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="RD444_002115"
     CDS             complement(11758..12897)
                     /locus_tag="RD444_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7549478.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="RD444_002116"
     CDS             complement(13206..13919)
                     /locus_tag="RD444_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDT7549479.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="RD444_002117"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="RD444_002117"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDT7549480.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="RD444_002118"
     CDS             15108..16640
                     /locus_tag="RD444_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7549481.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17006)
                     /locus_tag="RD444_002119"
     CDS             complement(16831..>17006)
                     /locus_tag="RD444_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7549482.1"
                     /translation="FMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQT
                     VVFKGKRNLENKK"
BASE COUNT         5278 a         3493 c         2910 g         5325 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aattcccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaata
//
LOCUS       DAPBAB010000054        16255 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPBAB010000054 DAPBAB010000000
VERSION     DAPBAB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.057"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(160..444)
                     /locus_tag="RD444_002120"
     CDS             complement(160..444)
                     /locus_tag="RD444_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549483.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="RD444_002121"
     CDS             complement(462..1352)
                     /locus_tag="RD444_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDT7549484.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="RD444_002122"
     CDS             complement(1372..1590)
                     /locus_tag="RD444_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDT7549485.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="RD444_002123"
     CDS             complement(1560..3260)
                     /locus_tag="RD444_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDT7549486.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="RD444_002124"
     CDS             3527..4300
                     /locus_tag="RD444_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549487.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="RD444_002125"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="RD444_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDT7549488.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="RD444_002126"
     CDS             complement(5124..6095)
                     /locus_tag="RD444_002126"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDT7549489.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="RD444_002127"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="RD444_002127"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDT7549490.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="RD444_002128"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="RD444_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDT7549491.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="RD444_002129"
     CDS             complement(9050..9874)
                     /locus_tag="RD444_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7549492.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="RD444_002130"
     CDS             complement(9847..10527)
                     /locus_tag="RD444_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7549493.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="RD444_002131"
     CDS             complement(10550..11488)
                     /locus_tag="RD444_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDT7549494.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12596)
                     /locus_tag="RD444_002132"
     CDS             complement(11733..12596)
                     /locus_tag="RD444_002132"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDT7549495.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="RD444_002133"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="RD444_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDT7549496.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..>16255
                     /gene="proC"
                     /locus_tag="RD444_002134"
     CDS             15452..>16255
                     /gene="proC"
                     /locus_tag="RD444_002134"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDT7549497.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         4758 a         3482 c         2786 g         5229 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaa
//
LOCUS       DAPBAB010000055        16187 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPBAB010000055 DAPBAB010000000
VERSION     DAPBAB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.058"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(296..1024)
                     /locus_tag="RD444_002135"
     CDS             complement(296..1024)
                     /locus_tag="RD444_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDT7549498.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="RD444_002136"
     CDS             complement(1002..2576)
                     /locus_tag="RD444_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDT7549499.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="RD444_002137"
     CDS             2805..3743
                     /locus_tag="RD444_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7549500.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="RD444_002138"
     CDS             3755..4666
                     /locus_tag="RD444_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7549501.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="RD444_002139"
     CDS             4690..6162
                     /locus_tag="RD444_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7549502.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="RD444_002140"
     CDS             6213..6824
                     /locus_tag="RD444_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7549503.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="RD444_002141"
     CDS             6854..8767
                     /locus_tag="RD444_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDT7549504.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="RD444_002142"
     CDS             8895..11876
                     /locus_tag="RD444_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDT7549505.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="RD444_002143"
     CDS             11873..13534
                     /locus_tag="RD444_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDT7549506.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="RD444_002144"
     CDS             13574..14506
                     /locus_tag="RD444_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDT7549507.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14865..16127)
                     /gene="guaA"
                     /locus_tag="RD444_002145"
                     /pseudo
     CDS             complement(14865..16127)
                     /gene="guaA"
                     /locus_tag="RD444_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
BASE COUNT         5168 a         2637 c         3278 g         5104 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 aacgttgatt tatcggcatt ccccatccat attatattta cgtcttattc ccactccact
    14881 gttgcaggtg gtttgctggt aatatcatac acgatacggt taacgtgtgc gacttcattt
    14941 acgatacgga cactgatttt ttgcaagact tcccaaggga tacgggcaaa gtcagcggtc
    15001 atgccgtcaa tagaagtgac agcacggata cctacagtgt agtcatacgt acggccatct
    15061 cccataaccc caacagagcg aatgcctggt aggacggtga agtattgcca gatgtcgcga
    15121 tctaaaccgg cattggcgat ttcttcgcgc aagatagcgt ctgattcacg gacgatttct
    15181 aatttttctt ctgtcaattc accaaggaca cgaataccta agcctggtcc agggaatggt
    15241 tggcgccaaa cgatgctgtc aggcatgcct aattgggtac ctaattcacg tacttcgtct
    15301 ttgaacaatg tgttcaatgg ctcgatcagc ttgaattgca tgtcttcagg taatccacct
    15361 acattgtgat gtgatttgat ggtttgggca gtttcagttc ctgattcgat cacatcggta
    15421 taaagggttc cttgtgcaag gaaagaaacg ccttcttcac cggcaagttt ggttgcttca
    15481 tcatcgaaga catatacaaa ttcatttcca atgattttac gtttttcttc tggatcagac
    15541 acgccagcaa gttttgataa gaaacgttct ttggcatcga cttttataat gttcaagcca
    15601 aattttccgc caagactttc cataacttgt tctgcttcac ctttacgcaa caatacgtga
    15661 tcaacgaaga tacatgttaa ttgatcgccg atcgcttttt gtaaaagaac accaacaaca
    15721 gaggaatcta ctccgcctga aaggcctaat aaaacttttt tgtcaccgac ttgttcacga
    15781 atttttgcga tctccatgtc gatgaagtta tccattgtcc agtcaccagt acattggcag
    15841 acatctagtg taaaatggcg caacaagtca tttccatatt cggaatggcg aacttctgca
    15901 tggaattgga ttccgtacat tttgcgttct gcattttgga tcgatgcgat ggggcaatct
    15961 ttgctggtag caacgacatc gaaatcagtc ggtgcttggg taactaggtc accatgactc
    16021 atccaaacgg tttgtttttc aggagtgttt tggaaaaggg ccgcttctgc agaagtgatt
    16081 tccaattctg cttttccgta ttcacggttt ttggctggtt taacagaatt aggcagaagt
    16141 cgcccatcct ctataggtgg gtgatgaatg ccgttcggta tgagggt
//
LOCUS       DAPBAB010000056        15939 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPBAB010000056 DAPBAB010000000
VERSION     DAPBAB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.059"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(173..475)
                     /locus_tag="RD444_002146"
     CDS             complement(173..475)
                     /locus_tag="RD444_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549508.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="RD444_002147"
     tmRNA           complement(695..1061)
                     /gene="ssrA"
                     /locus_tag="RD444_002147"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1232..2005)
                     /locus_tag="RD444_002148"
     CDS             complement(1232..2005)
                     /locus_tag="RD444_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7549509.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2149..2499
                     /locus_tag="RD444_002149"
     CDS             2149..2499
                     /locus_tag="RD444_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDT7549510.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2480..3196
                     /locus_tag="RD444_002150"
     CDS             2480..3196
                     /locus_tag="RD444_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7549511.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3196..4644
                     /locus_tag="RD444_002151"
     CDS             3196..4644
                     /locus_tag="RD444_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7549512.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4714..5223)
                     /locus_tag="RD444_002152"
     CDS             complement(4714..5223)
                     /locus_tag="RD444_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549513.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5213..5938)
                     /locus_tag="RD444_002153"
     CDS             complement(5213..5938)
                     /locus_tag="RD444_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549514.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6161..8281)
                     /locus_tag="RD444_002154"
     CDS             complement(6161..8281)
                     /locus_tag="RD444_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7549515.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8511..9689
                     /locus_tag="RD444_002155"
     CDS             8511..9689
                     /locus_tag="RD444_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDT7549516.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9705..10463
                     /gene="aroD"
                     /locus_tag="RD444_002156"
     CDS             9705..10463
                     /gene="aroD"
                     /locus_tag="RD444_002156"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDT7549517.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10486..10971
                     /gene="ybaK"
                     /locus_tag="RD444_002157"
     CDS             10486..10971
                     /gene="ybaK"
                     /locus_tag="RD444_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDT7549518.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11042..12295)
                     /locus_tag="RD444_002158"
     CDS             complement(11042..12295)
                     /locus_tag="RD444_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7549519.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12412..13761
                     /gene="gdhA"
                     /locus_tag="RD444_002159"
     CDS             12412..13761
                     /gene="gdhA"
                     /locus_tag="RD444_002159"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDT7549520.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13873..15219
                     /locus_tag="RD444_002160"
     CDS             13873..15219
                     /locus_tag="RD444_002160"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDT7549521.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15527..15913)
                     /locus_tag="RD444_002161"
     CDS             complement(15527..15913)
                     /locus_tag="RD444_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7549522.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5217 a         2844 c         3191 g         4687 t
ORIGIN      
        1 cttataattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
       61 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatccctttt
      121 ataagtatgg tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt
      181 tatacctttc tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc
      241 gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg
      301 tttttgttgc cggaatttta aagtagttct tattagagta atagagattg attaataagc
      361 tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa
      421 atctattcga aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt
      481 tcacctcaaa aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata
      541 atatggatgg aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt
      601 ttcattcatt taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaataagg
      661 atttaagcat ctagcgaaat ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc
      721 gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca
      781 ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa
      841 cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg
      901 ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt
      961 tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc
     1021 aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac
     1081 gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga
     1141 atcatatccc attatttata taataactgc ccctttctta agaaaaaata agcgattttc
     1201 ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt
     1261 ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt
     1321 cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg
     1381 gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt
     1441 tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc
     1501 gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga
     1561 tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg
     1621 aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct
     1681 gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac
     1741 attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac
     1801 cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt
     1861 ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt
     1921 ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga
     1981 aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa taagtttatc
     2041 ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca
     2101 gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc
     2161 agtattggta tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga
     2221 aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg
     2281 ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc
     2341 ggctattatt taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta
     2401 gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg
     2461 gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga
     2521 aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa
     2581 agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct
     2641 gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac
     2701 tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga
     2761 gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat
     2821 gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag tagtttcaga
     2881 tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta
     2941 tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt
     3001 agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta
     3061 tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat
     3121 cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga
     3181 ttctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttctg cggaatggtc
     3241 gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa
     3301 gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc
     3361 tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg
     3421 acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt
     3481 gtcatttatc cgactaatac agctttactg aattttacct taacagctga tcaatggaaa
     3541 aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca
     3601 caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt
     3661 gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg
     3721 ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca
     3781 ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat
     3841 tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc
     3901 aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt
     3961 cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac
     4021 ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt
     4081 aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat
     4141 gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca
     4201 ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca
     4261 ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg
     4321 gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta
     4381 gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa
     4441 caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca
     4501 aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa
     4561 ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct
     4621 gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc
     4681 tataccgctt gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat
     4741 ataaggactt tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc
     4801 atgagctaaa taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc
     4861 aaatgtatct tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag
     4921 ctttactttt tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat
     4981 atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa
     5041 caggacatgg tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc
     5101 aaaattatcc agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg
     5161 tttttcgaga ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt
     5221 cattacgtcc aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac
     5281 taagaatgag acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat
     5341 ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa
     5401 ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta
     5461 agacaagaaa attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc
     5521 acaataaaac caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa
     5581 ggaataattt ggttttagaa agaccagtca ggtgccaatt gatcaattcc tgcttgtttt
     5641 gcctgactga atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga
     5701 atatgccttt tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg
     5761 cagaagcgcc aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat
     5821 ggtaaagagt caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga
     5881 gtattgtctg ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac
     5941 ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt
     6001 gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg
     6061 ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag
     6121 cgacaataat tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg
     6181 tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt
     6241 tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt
     6301 tctctaagcc attcttgtag tcataatcac tggtatcaat tggatctaat ccactattcg
     6361 tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa
     6421 ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt
     6481 caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat
     6541 caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact
     6601 gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt
     6661 gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag
     6721 ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta
     6781 attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga
     6841 tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg
     6901 tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta
     6961 acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa
     7021 tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa
     7081 atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag
     7141 aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac
     7201 cagcgttccc atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg
     7261 cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa
     7321 acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga
     7381 aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa
     7441 cttcatgctg gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc
     7501 tttctaaatg gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg
     7561 gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt
     7621 ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca
     7681 agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat
     7741 cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa
     7801 aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa
     7861 cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg
     7921 cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga
     7981 agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat
     8041 aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa
     8101 gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt
     8161 cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga
     8221 ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca
     8281 agataatctt cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag
     8341 ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt
     8401 ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt
     8461 cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa
     8521 aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag
     8581 accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc
     8641 aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc
     8701 agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa
     8761 aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag
     8821 gagacgggat cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt
     8881 ataacgaaac gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc
     8941 cggaaatcat aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc
     9001 agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat
     9061 ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac
     9121 ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata
     9181 ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc
     9241 tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg
     9301 atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac
     9361 aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag
     9421 tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag
     9481 aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa
     9541 caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc
     9601 gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt
     9661 ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg
     9721 gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag
     9781 aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc
     9841 gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta
     9901 tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg
     9961 gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag
    10021 gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga
    10081 tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa
    10141 caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata
    10201 tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga
    10261 ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg
    10321 gtaaaatttc tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa
    10381 gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc
    10441 aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa
    10501 atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac
    10561 gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa
    10621 agccaatctc gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt
    10681 gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat
    10741 aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt
    10801 ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa
    10861 agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct
    10921 caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt
    10981 ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac
    11041 attatgctct ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga
    11101 tattaaagcc gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat
    11161 caatgatttt actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag
    11221 ttacaaatga tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata
    11281 gttgaacaaa atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt
    11341 caagccgctc aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga
    11401 ccaagttctt tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg
    11461 tttgtgtagg gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt
    11521 tattgacaaa acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa
    11581 gtaaatccat cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga
    11641 tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca
    11701 agggggactc tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt
    11761 acccatctcc agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg
    11821 caggagcatg gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga
    11881 tcaatttctc tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga
    11941 gcaatgcttc gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct
    12001 cttcttttaa atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt
    12061 gcgcaaacgt actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa
    12121 caggacgatt gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg
    12181 cttttttgtt tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg
    12241 ccatcatccc gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct
    12301 cacctcaagg aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca
    12361 aaagagaaaa tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac
    12421 gcaacagaat atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa
    12481 gaatttttac aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat
    12541 cccgaatata tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa
    12601 tttcgagtgc cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc
    12661 caatacaact cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat
    12721 ctaagtatct tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg
    12781 ccaatcggtg gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag
    12841 atcatgcgtt tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt
    12901 gatgttcctg cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa
    12961 tataaacgat tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg
    13021 ggaagtaaga tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta
    13081 ttgaacgaag aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat
    13141 gtagccattt atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca
    13201 gattctagcg ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc
    13261 aaagaaatca atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat
    13321 catgctggag aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct
    13381 actcaaaatg aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc
    13441 gtagcagaag gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca
    13501 ggcgtgtggt attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta
    13561 gaaatgagtc agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg
    13621 gatcaaatca tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac
    13681 gaaaaaaatc tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg
    13741 gcagcagaag gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt
    13801 tatttgcttg aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa
    13861 ggaggagaac aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac
    13921 gaacaagagt tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa
    13981 ggtactggcg caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa
    14041 gaagaatttg cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta
    14101 gttgttatcg gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat
    14161 cacacattct tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc
    14221 ggaaattcaa tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac
    14281 ttttcagtaa acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt
    14341 gtattcaaag agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac
    14401 gctacaacag ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa
    14461 actttcgtga ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg
    14521 ttaccaatcg ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca
    14581 agcaaagcat actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg
    14641 cgcaacattc tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga
    14701 atgcaatact tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa
    14761 aaaggcatct atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc
    14821 atccaagaag gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa
    14881 tcaatcacaa ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa
    14941 gaagtggact tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt
    15001 gacgtgccta acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca
    15061 atgtacttct ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc
    15121 gaccaaccag gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc
    15181 tttgaagatc ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat
    15241 ttaaataatc agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt
    15301 actcaaacat actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag
    15361 tcccttgttt gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta
    15421 agaacagtga ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag
    15481 caaaaaagca ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta
    15541 atttttcttt tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc
    15601 gcttttcgct tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag
    15661 agtcaccaga agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag
    15721 catctacgtt gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag
    15781 tttctgcagg cattggagct ttgactaccg cataataatc tttacctaca taagtagact
    15841 gatagtagtc ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta
    15901 aaattttttt catgaaagat ctcctttttt atttttatt
//
LOCUS       DAPBAB010000057        15354 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPBAB010000057 DAPBAB010000000
VERSION     DAPBAB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.060"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(21..251)
                     /locus_tag="RD444_002162"
     CDS             complement(21..251)
                     /locus_tag="RD444_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549523.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="RD444_002163"
     CDS             complement(687..1253)
                     /locus_tag="RD444_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7549524.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="RD444_002164"
     CDS             complement(1253..2209)
                     /locus_tag="RD444_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDT7549525.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="RD444_002165"
     CDS             complement(2209..2865)
                     /locus_tag="RD444_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDT7549526.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="RD444_002166"
     CDS             3235..5253
                     /locus_tag="RD444_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7549527.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="RD444_002167"
     CDS             5680..8094
                     /locus_tag="RD444_002167"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDT7549528.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="RD444_002168"
     CDS             8442..9995
                     /locus_tag="RD444_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDT7549529.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="RD444_002169"
     CDS             complement(10376..12160)
                     /locus_tag="RD444_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549530.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="RD444_002170"
     CDS             complement(12175..13896)
                     /locus_tag="RD444_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549531.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="RD444_002171"
     CDS             13996..14757
                     /locus_tag="RD444_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7549532.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4998 a         2802 c         3007 g         4547 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gttt
//
LOCUS       DAPBAB010000058        14754 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPBAB010000058 DAPBAB010000000
VERSION     DAPBAB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.061"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..152)
                     /locus_tag="RD444_002172"
     CDS             complement(<1..152)
                     /locus_tag="RD444_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7549533.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            235..384
                     /locus_tag="RD444_002173"
     CDS             235..384
                     /locus_tag="RD444_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HDT7549534.1"
                     /translation="MKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPL
                     ENELR"
     gene            complement(1133..1746)
                     /locus_tag="RD444_002174"
                     /pseudo
     CDS             complement(1133..1746)
                     /locus_tag="RD444_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2967..3671)
                     /locus_tag="RD444_002175"
     CDS             complement(2967..3671)
                     /locus_tag="RD444_002175"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7549535.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            complement(3686..4555)
                     /locus_tag="RD444_002176"
     CDS             complement(3686..4555)
                     /locus_tag="RD444_002176"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDT7549536.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            complement(4663..5679)
                     /locus_tag="RD444_002177"
     CDS             complement(4663..5679)
                     /locus_tag="RD444_002177"
                     /inference="COORDINATES: protein motif:HMM:NF013539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7549537.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            5869..8592
                     /locus_tag="RD444_002178"
     CDS             5869..8592
                     /locus_tag="RD444_002178"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7549538.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKSKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            8720..9142
                     /locus_tag="RD444_002179"
     CDS             8720..9142
                     /locus_tag="RD444_002179"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HDT7549539.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            9160..9630
                     /locus_tag="RD444_002180"
     CDS             9160..9630
                     /locus_tag="RD444_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549540.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            9654..10436
                     /locus_tag="RD444_002181"
     CDS             9654..10436
                     /locus_tag="RD444_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549541.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            10420..11250
                     /locus_tag="RD444_002182"
     CDS             10420..11250
                     /locus_tag="RD444_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014093338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7549542.1"
                     /translation="MKMTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMM
                     PALEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTI
                     TSIKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLR
                     YFGVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQG
                     DEATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT
                     "
     gene            11285..12283
                     /locus_tag="RD444_002183"
     CDS             11285..12283
                     /locus_tag="RD444_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7549543.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            12300..13154
                     /locus_tag="RD444_002184"
     CDS             12300..13154
                     /locus_tag="RD444_002184"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549544.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(13728..14754)
                     /locus_tag="RD444_002185"
                     /pseudo
     CDS             complement(13728..14754)
                     /locus_tag="RD444_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         4724 a         2331 c         2840 g         4859 t
ORIGIN      
        1 acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatataa caattcctga
       61 acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat aatgacgtct
      121 tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc ttttttctca
      181 attttacact aaaatagatt ttttggaaaa ctttgcaaca gaaccatttc taatatgaaa
      241 gcaattatca acagtaagga ttacgaagga tataaagcca aacatccaga agcgaaacca
      301 tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa tgttagatga tgagttgatt
      361 ccacttgaaa atgaattgag gtaaatgaga agagttaaga aaattgtacg attcaaagat
      421 agtagtaaag tggaagtgta gaaaataggg atagatgtct tttatttgtt cacattttat
      481 aacgaagtat ttaagacagc tattgaataa tgtttcctcc acctttttca ttcatttacc
      541 ccaccccgtt gttatatgac cgtgtttcga gaggaaccaa taaaaagcat aggctaacta
      601 ggggttcctt tttcttttga ttttttggaa tccgcttaag tgaaatgtat ctgaaatgaa
      661 acagagataa agtgattttt tgctgtaatg acaaggtttt agctgtttca gataccatgt
      721 aacccaaaat agtttattga gaaaatagga gagtatttga tactaactag taggaaaaaa
      781 tgagataaaa agtaaatgga atcatttcag gtaatctcaa ataaaaaata tacaggcctg
      841 tggcacttta cctacgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      901 gtttgactcc gtcgtattga ttttaaatga tattcgatga ccaattaata ggtcgttttt
      961 gaaatttttg tctctatatg actcagtttg ataattattg atagatcgat ttgggtctat
     1021 atcgaacaag ggaagattgc aaaagtcgaa gcttaaagga ttgatattaa aggattaaga
     1081 ggtaaaaagg tatccccata atatcctctg actccgtttt tgacctcgct tatcatccgt
     1141 tcatgtaaga atcaaataat tcgactgttt cattttgccg attttttgat actttaatat
     1201 aaatgtccat gattgtttta gaagaagaat gtcctagcct atcttgtaca tcttttactt
     1261 gagcacctgc ttcaaagaga agggtagcat gagtctttct gaagccatgg actttgatta
     1321 tcttcatatc tgggaacttc cgtgctactt tatccatgac atggttaggg tagcctttgt
     1381 aaaggaagtc ccttttttta ttccctttga tagtgctgat tacaggtata tcatctatat
     1441 tatctatccg aatccctcta gaaaggttct tttatgttac agaacgtttc cattttttag
     1501 aatacgtaag gttccattat caattgaaat tactcccttt gatttgtctg tttttgtagg
     1561 attaatacat agagagccct cggatagttc tactaacgtt ttgttgaagc agatagtttg
     1621 attatcgaaa tctatgtctc tacatgtcag tccgagtatt tctccttttc gttcccctgt
     1681 ataagaagct attctaaaga atactaaagc acgctcatct tttaacttat gcaaacattc
     1741 aaaaaacaga tatttcacat atatctaaaa ttcggagatt gaaagaatga atttatctaa
     1801 gaattttcac taatgttgcg aatataatat cagaaaattt cgaaataaat tttgttaaaa
     1861 attagaaaaa tccgaatact tagtagtgga taaatttttt tcaggaatag tacgttttat
     1921 taaaatgaaa actaatccga catcaacttt tttatcgaag caaacatcaa gcggtaaaaa
     1981 gacctcttta gtttgaaaag tgttgctttc atattgattt aaattttggt taagtaggta
     2041 aattaagaaa tgatcagtgt aagtatttcc tgatttagcg caccttgttc actttgaaaa
     2101 tacaggtttt tgatggacag attttgcttc aaatgtttaa agaacatgcc actgtacttg
     2161 gtacaggtcg gcgcccccat ttggagttgc gtcagatcga gtcgtgacga ttcattttgt
     2221 acaaattaga atttaaaatt gaatacatga cgcgcacatt ttcccagaga gccatattct
     2281 gtaattaatc acagcaatta gtgattatgc acaatctaaa aaactgattt ataataaaaa
     2341 tattattcaa ataaataata ataactgccc gcatgatgtt gacagttaca atagcacata
     2401 gatagactgg caaagatggt tcttaatctt tcactttggt cagaattctt gggttatcct
     2461 tccagtgaaa gtcagcagaa aatccttaat gcttacaaat aatttttgcg aaataggttt
     2521 tattggtaaa ggactcaatc atgaatatga attactttta tctggtaaat ttggaaaaat
     2581 tttgcaaaga aataaaaatt agacaaattt gctacacata atcaaagatt aaaaaagttt
     2641 ttaaaatata gttgtttgaa tggggatatg aattagagaa aattggttga ttcaacgggt
     2701 gctatcattt ttgatagcgt catatgtgaa gtaagcacac ctaatgaaaa aggttcaggc
     2761 ttgctttgaa gacttattaa caactatgtc gactatcctt gacttagggt agaggagtac
     2821 ctccacacac cttaattaga tgataatgtt tggactgtag gctacttagc ggattaagat
     2881 aaagaggaag tagaaaaaga gccttgcgga aagacacttt atctgttatc cgcgaggctc
     2941 tttatacatt taagcatatt taaaaattat ttggaaacag tactttcaaa ctttgtctta
     3001 taagcagggt acttagatat ttcatattca aacggtttct gatttttagc aaatccaata
     3061 cttgtaaaga caaagactgg ctcatttttt tctaaatcaa gataaaggtt ttctaaatct
     3121 gttggcggtg taacttcaat tgagcgtttg gcataagcta atctaattcc cctactttct
     3181 aatactgaga acagggattc tttggcaaaa tcatgttcaa caatttcagg taaaacagat
     3241 aggggaatgc aactttttgc aatacaagtt ggatagtcat cgatgtatcg aattctttcc
     3301 aagacaagca ctaaagtatt ctgctcaatc tccaaacatt cactaattgt tgaatcagca
     3361 ggtatgatgg tttgttggat aatcttagaa ctgattttct ttccttgctg aaccgcatct
     3421 tggaagaatc cgataaaaga gtcgagttcg tggtcaacat atttttctga agctggtttc
     3481 actagatacc cgcgaccttc atttttagaa ataattcctt cgtcttctaa agaagctaaa
     3541 gctttccgaa ttgttggacg actcaccgaa aattcctggg ccaaatctct ttccgaaggc
     3601 aataattcag atttccgatc agtggtgata tacacttttc gaatttgttc ttttactttg
     3661 tcaaaaatca tagtaatcca tccttttacc aaggtattaa cattatatta ccattttttt
     3721 gacttaatat cccagatttt ccttcaatta attccttcgc cgctaattga ccccacaaat
     3781 agccaatata tcggtcataa aaagttggag atgatccacg ctgaagatac gcaagattta
     3841 agtatttggt ttcttttggt aaatttttgg taataaattc aatgattttt tgtttgttag
     3901 atataccttc tggtaaaagc aatataggcg agtagaactc cgactcaaat tttttacata
     3961 ttaagtgtaa gtattccttg tcgtctctct caacccaata agtattattt gcaatggaag
     4021 cgtactctgg aagagcaaag cagcctcggc ccatgacttc cattataata atacgatcat
     4081 gagcattagc agtagaagtt aaatcatcga ttgatcggat tatagtgttt aaggcgctat
     4141 cgaagccaag tgtataatcg gtattgggga aatcgttatc aatagtccct gggatgacag
     4201 atacttgtaa ttctgattca tttttcaata gttcagctcc tttcaaggaa ccgttacctc
     4261 caataacaat taagttgtcg atctccatct tatttaggtt ttcaataata attttccgac
     4321 tttcgagatc tctcattttc tcaaatctgg ctgttccaat aagtgttcca gctttgaata
     4381 cctcaacttc ttttagagaa actgaggcaa tacattcctt aaatattccg gcataaccat
     4441 tcttaaaaaa gactacttcc gaatctggtt cgctatcaat tgtttttttt attgctaata
     4501 aagctgaatt cattccggga gagtcgccac cactacagat aactgcgatt ttcattttag
     4561 atcctcctaa aaaaggtgac tgcgattcta ttctcccatt agagaaaatg aagcaatcaa
     4621 atgggcaatg gagtcgcggt cacctttttc atatttttat gattagtatt ccatttgctt
     4681 ataataacgg cgagtagtta aagggtggtt acgttcttct tctagaaaag cactaattcg
     4741 ttcaaagaca gtagctaaga cgactgggga aataattttt cgcgtgtctt ccgaaatgcc
     4801 aggtaagtca tagtcctttg tatcaaccgt tacgtgaaca tctgtaaact taggaacgaa
     4861 attcttcacg cgttctgtga gaggtcgaga atcatcttct ccttctgtaa tgatgacact
     4921 tacacccgct tcaattaatt caagagttcc atggaagaag tctgctgcat gaacaggacg
     4981 agtcttaatc cattgcatct cttctaaaat acacatgcca tagtaatatg cttccgcaaa
     5041 gctatcacca gcaccggtta aaatatgata tgaagtatct ttgtgttttt ttgcaaaaga
     5101 ttttgccatt ggttccgctt gctctttagc gtgcactaag gcttcaggta ttttttttaa
     5161 atcctcaata taggtattgt attgattgat atcgatttca tttctttgtt tcattaagaa
     5221 gagagcaatc agcagtcctt gaaggtaata attttctgaa gatgtatcat ctaaaacgtc
     5281 gttatagata gaatatgttg cagctttttc taggggcgtt ttaggacttc ctaccaaagc
     5341 taatgttttg acgttcattt cattacagta ttcgactaac tcaagtgttt cttttgttgt
     5401 accagataaa gatgggataa caaccaaaga tttgttagaa aatgttttat tgggatgaat
     5461 cataaattcg gcagcgataa cacgatgaaa agggaaagtg ctacgagttc tcgcgaattc
     5521 gattgttgaa tcaaaaagaa taccaactcc tccagaccca ataaagtaaa tttcttctaa
     5581 gtcagatgcg atggattcta aaatattgga aatttgttca tttgttgcaa cggcaccagc
     5641 ttgagtcttt aaaaatcttt cttgatcaaa attaaacata aaaatctctc ctttgattac
     5701 caatggtaat attttgtttg cataaataaa ataccacagg taataaaaag tgtcaaatat
     5761 tttttaggaa aaataccata ggtattgtta aagtggaaag atgtttataa agaatattga
     5821 attttatagt gaaaatcatt tattattaag taaaggaggt gggttcattt gaaaacgaaa
     5881 attttaaaag aattaaatga tctacttaac agctatgatt ttccacaata ttttccagaa
     5941 gaatgtaacg cgcaatatat tgcccaaaaa tttcaaataa aaagaaatac ggctagtaag
     6001 catctaaacg aactaatgga acagaaaaaa gttgtgaaaa tcaatggtcg tcctgttctt
     6061 ttttttccag tccatacaat tgaacggtat atatcacatt cattagatag ttttgagttt
     6121 tcttcaattg attcgtttag agaaaaaatt tcaaagaaaa aagcagatca tttttttgat
     6181 gatttaattg gtgcaaaaca aagtttaaaa gaagcggttc aacaagggat atcagcaatg
     6241 aaatatccag gtggattacc aataatgtat tttggatcgt ccggagttgg gaagagttac
     6301 ttagtggaaa aactagctga gtattgccga caaactggta ttattcatga gaatgctccg
     6361 ttcatcgagt taaactgcgc tcagtatttt aataatccag agttattaac aagtcagttg
     6421 tttggccata tgaagggatc gtttaccggc gccgattctg accatattgg tataatagaa
     6481 gcagcagacg gtggaatcgt ctttcttgac gaaattcatc gtttaccacc agaaggacaa
     6541 gagaaacttt ttattcatat ggataaaggc gtttttcgaa gagttggaga tagtggtcct
     6601 tggcgtttat cacatgttag atatattttt gcgacaacgg aacaggaaga aaattttttt
     6661 ctagaaactt ttcgacgaag aattccgatt gtttgtactt tgcctaatta ccaagataga
     6721 gaaattagcg aacgaaaaga gattgtcttc catttattag agaaagagtc tactctgatc
     6781 aataaagaaa tattagtttc tgaagccgcc cttagtttcc tgattcgctt agatttgtat
     6841 ggaaacattg gtgagctgga tggactaata aaacaaacta ttgcgaataa actatttagt
     6901 ggaagcaata aaaaatatat cgaaattcat atttctgatt tacctagcag gtacttgcta
     6961 acagaaaata ttgagttaaa aaaccaaatt gcgagccaac ggttctatat ctttagtaat
     7021 ggtaactatg ttcatgattg tgacgagact tcaactgtaa ctacaaattt acgaggtttg
     7081 attcatcgtg tttctaaata ttattcagag tgtcatgatg tgaatagaaa tagtgaagag
     7141 tttcgacgtt ttgtgaagga ccaaatggca gaatttaatg atcgttttat ttaccaaaaa
     7201 aattcagaag tcttagttaa gtattattta ccggaaatcc aaaatattgt tcaaaaaatc
     7261 tctcccagtc tttttgttca cctttctggc aatacggtcc aagctatagc tacctatctc
     7321 gctattaggg atcaatgggg gtatgggaaa gagaatgctt cagcgaatga aattgaaaag
     7381 ttatatgaaa acctaaagtg taactatttg acggatttaa atgtattctt actgatcttc
     7441 gagaacgccc tggatacaac attgactatt tgggataaac tgtatatcag tttgtgtgtt
     7501 attagtgaga ccggaaatga agcgaaagac attaacgcaa ttattttagc ccatggatat
     7561 gcgacagcta gtagtattgc taatgttgcc aatcgtttgg ttaatcagtt tgtcatagat
     7621 tctattgata tgcctctaga tatcagcttt agtgaagtaa ttgacagatt acttcactat
     7681 atagagtatc gaaagtctaa agatggacta gtgattttcg ttgatatggg aagtttagct
     7741 caaattaaga ctgaaattga acaagtaatc gaagttccaa caatgattat taataatgtt
     7801 acaaccgaaa tggctattga aaccgcacaa ctgattcaaa gtacgagtga tatccaaaag
     7861 gttgtaaaaa aattgccgta ttctcagttt gaaaagcaag ttctatatcc catcaaaatt
     7921 agaaaacgta caatcgttgt ttcctgcaat actggtttgg gtacctccat aaaaattaaa
     7981 gaaatgatgg aaaataattt gtcgaaagac ttgggcattg aatttctgcc atacgaaaat
     8041 gaaactctta gagatactca gcaattagag tttctcatta aaacaaagga tatcattgcc
     8101 attatcggaa cagatgatcc gcgtgtgtcg aacattccct atatttctct tgaagaatta
     8161 tttgatggtg aaattggcaa actctataaa gttcttttga gagtctatgg aaaagagatt
     8221 gctgaggcaa ttaatcaaaa tattgttaaa aatagctcat taaaccgctt gattgacagt
     8281 cttactattt tagatgccga aaaagttgtc aatcaaattg aggaaagttt aaaagattat
     8341 caaatgaggg tggggaaaac gttatcatca ttgtccaaaa ttaatcttta tgttcatatc
     8401 agctgtatga ttgagcgact aattcgtaat cagggagtaa gtagttatcc agcaatagat
     8461 cagtttctta agcgatacga aactgaggtt gcttctattc aaaaggcact tagtaagatt
     8521 gagaaacttt ataatattaa gattcctatg gaagagattg catatgtgtg caacatcatt
     8581 gtaagttatt gataaaataa agtgacacta ttcacagcga tggaaatagt gtcactttat
     8641 tttttgtacg ccctttcatt taagtggtga cagtaattgg cacagtattt gctaataaca
     8701 aagcgaaagg agttcgggta tgagacaaat attatgtatg agccacggtc ctctagcaaa
     8761 aggaatgatt gaaactttga gtattatcgt aggagatttg gataatattg attttcgatg
     8821 tgcctatgtc gatggaaata atgacgtaga aatgatcatc gatgattatc taaaggaatc
     8881 tatgggaaat gagacgattg ttgtaaccga catttttggt ggaagcatta ataatgagtg
     8941 gctaagacgg ttgccagatc tttctaaagt ctatttgatt tctggaatga atttaagtct
     9001 tttagtggat ttatatttaa aattccaatt agtttcatct gatcagattg aagaaattat
     9061 tgatcatgca attcaagcat cttctggttc gattaagttt tgtaatcagc tagattatga
     9121 gcaaacggtt gatgactttt aaataaagga gggaagagaa tgattaaatt agtgaggatc
     9181 gatcatcgtt tgatacacgg tcaagttgct ttaacttgga cgaacgcaat cagtgcggat
     9241 tgcattttag tagcgaacga tgaaattttg aatgatccaa ttcaaaagac aactttgaaa
     9301 ttagccgcac cacataacgt gaaggttgtt atcaaatcaa ttcaagattc gattgatgca
     9361 attaatagtg ggaagacaga taaatacaaa ctatttgtag ttaccaaaac catttccgat
     9421 gctaaacggt tggcaaatgg ggttgaacaa attaaatctg taaatatcgg tagtgttaag
     9481 gaaactagtg gcagtagcgc tttgacgcat acaatttttg taacagaaga ggaacgagca
     9541 gaacttaaag gaatggtctc agagggcatt gaagtgttcg ctcaacaagt tccaacagat
     9601 aaggcaatag atttaaaaaa atctttgtaa taaaaaaata agggggtaat ttcatggata
     9661 gtctcttagt acaagcaatt ttgcttggtc tcttgactgc gttcatcgta attgactgga
     9721 tgacaggtac gaataactta tcaagaccaa tcgtatcttc cgtctttgtt ggtttaatta
     9781 tgggcgatct taagatgggg attatgatgg gggcaacact ggagttagca tttatcggag
     9841 caataacaat cggagcggca cgtccgcctg atacggtatc tggtgggatt ttagggactg
     9901 cgtttgctat tgcaactggc aagggagcag aatttgcgtt gaccttggca ttcccaatcg
     9961 ctgcgctatt cttattagtt gataatctac taacattgct tgttctacca atgattgcta
    10021 gaaaggcaga tagatatgct gaaaagggcg aactaaaaaa tgtgtcgaaa atgcatgttt
    10081 taggttttat cttagtaaaa agtttaccac gggctttatt tgtcggattc tcattttact
    10141 taggaagtcc tgttatggaa cgaatcttaa atgctattcc aacttttgtc caagatggga
    10201 ttaacattgc tgcaggcatt atgccagcac taggttttgc tattttattg caaatgcttc
    10261 ttaagaaaga tgtctttgta tatttcgtaa ttggttttgc actctttagc tacttacatg
    10321 tgccgattct ggggattgca atcttcggcg catgtctagc ttatattata gtaaacatgg
    10381 aaaccaaagc tgcaatgggt tcaagagggg aggagatggt tgaagatgac ttctagtggg
    10441 aaagctttaa ctaaagatga tttaacaaaa atttgtattc ggtcatgtgc attagaagca
    10501 tcatggaatt ttgagcgcca acaacatatg ggctttgctt ttgctatgat gccagcgtta
    10561 gaaaaaattt atagtggcaa tgaagaagaa aagcaaaaag cagctcaaag acatctagag
    10621 ttttttaaca cagctccgcc attatcaaca ttcataatgg gaattgtggc ttctatggaa
    10681 gaagaaaatg ccaaaaatcc tgatgacttt gatacttcta caatcacttc tattaaaact
    10741 gcattgatgg gaccattagc tggtattggt gattcaatgt tttggggtac cttattagtt
    10801 atcggtatcg gtgtaggtgc ttcattagca aaagaaggca atattttggg tccattttta
    10861 tttttactaa tcttcaatgt ccctaatttt cttctccgtt actttggagt tatgcaggga
    10921 tataactttg gtacaaagct attagagaac attgaatcct ctggcgtcat gaaaacgttg
    10981 actcatgctg cctcaatttt gggtcttatg gtaattgggg gcatgtctgg aagtatggta
    11041 tcaatcaaag tagctggttc tattggacaa ggtgatgaag caacaaaaat tcaaagtatc
    11101 attgatggta ttttaccaaa tctgctgtca gtattatgtg tatttctgat ttactggatg
    11161 cttaaaaaag gctttaaaac aacttacgtg ttattcggaa tctttatctt tggtatcata
    11221 gcagccttct tcggattttt gacaacttaa ttgaataatc taaataattg taaggagaga
    11281 actaatgtta aactataatg caaaagaaat gaaaaaaaaa ggcaaacgga tttacggtat
    11341 tcgtgaagat gtagaaaaaa ttgcaaagca agcttatgat tctggaatta gtaatatttt
    11401 ctttactgct tctggtggat cggtagccgt aatgcaacca tttgagtatg tattgatgac
    11461 tgaatcaagt attcctgttt actctatgac tgcagctgag cttgtcttaa tgggacataa
    11521 acagttgaat gaaaaatctt tagtaatctt atactcaaaa tctggagata cgaaagaaac
    11581 tattaaggca gctgaatatt taaaagaacg aaacgtgcca accgttggta ttgtaagtaa
    11641 agaagataca ccgttagagg ctctatcaac ttatgcaatc aattacctag acggccgccc
    11701 tcaagaaatg ccactttatt tcttaattat gtctatcatg aatttgaatg gggaatttcc
    11761 tgaatacact cgttttgctg atgaactgaa aaacttacca acggctctga attcagttag
    11821 agaacaggct gatgaaaaag ggaagaagta tgccgaagaa tttaaagaag atccttatca
    11881 aatttggatt ggtagcggga atttatgggg aatcacctac tcttattcaa tgtgtgtgct
    11941 tgaggaatct caatggttgc gtacaaagtc tgtctcatca ccagagtttt tccatggtac
    12001 cctcgagtta gtcgaaaaag gagtttgtgt aacacttttg aaaactgagg gggaaacacg
    12061 gcttttagat gctcgagttg aaaaatttgc ggtccaatat acagataaat ttaatgtttt
    12121 tgatactaag gattatgagc taccaggtat tagtgacgaa tttcgaaagt acttttgtgc
    12181 tccagttatg tgggcagccc taggtcgcgt cagtgttcat cttgaagata ttcggaacca
    12241 ctctttggat aaacgtcgtt attaccgagt agttgaatac taaaagtagg tgattaatta
    12301 tgacagcgta tgtgattgga tttggcgata atgttgttga ttactatgag aatttgaatc
    12361 aaaaatttcc agggggaaat gcagttaatt ttgctgtgaa tgcaaaaaag agtggtgtta
    12421 aagcatacta tgtcggtagt ctatcatctg atcctgatgg cgtgttatta caacacgcca
    12481 tcagttctga aggtgttgat atctctttct cggaagttat tgacgagcct actgagcaag
    12541 cacatgtaac tattaaagat ggagataggg agtttatagg tggcgtgcgt ggctcccgat
    12601 tgacacctgc tctctcagca gaacttattg atttgatgaa gaacagttca ttagttcata
    12661 ctagttgcca tagtcgaaca gaaacaaaaa ttaaagaatt aaaaaaatat gatgttaaag
    12721 tttcttttga tttttctgac ttagataaat atagaacgca tgaatacttg tcagatgttt
    12781 gtccgtcaat ttatattgca cagttttcag tcgctacaga tgatcaagag gaaattaatc
    12841 gcttaattag tgaatgcaag gactttgagg ttccgtatct tctttttacg cgtggtagta
    12901 agagccctgt ttttgttgac actcgagaga ataagactta tgaaggattc attcgcaaag
    12961 ttgagtcacc tcatgataca atgggcgcag gtgatgcata ctttgcagct tttgcaagta
    13021 attttgtctt atctaaacgt gaaaaatcaa tgatagaaca tgttattgat tgttttcaca
    13081 aaggtgcaaa ccttgcatat gaaacattga tgattgatgg ctcctttggt catgctacac
    13141 ctattaaccc ttagctttgt gctttgggct tttattgctg gaatttgtat agtagactca
    13201 tcataactat atttttgtat gtgaaaaggc aagaggctat gatgaaaata acactggcta
    13261 attgcatgag ggagcatatc gttgttaaag cgtcattgat agtatgaata aatatctcga
    13321 taatattata ttgttattta cttaatttat tgattagtca agcaaagata atcttctgtt
    13381 acttaacgag actagtaggt acaagcactt catgttatgt acgacccaaa aatattgctg
    13441 ttgattattt gctgaaatta ttgcgagctt caatttattt ttaaaggact tggtaggctg
    13501 cgtgatgaaa taagacttgg aaggctgtct aaaccaatat atagtagact aaatagtaaa
    13561 aaagagcatg gtaattgtct ctttgtcaat aagtctaatg gtaataagac aagttggaga
    13621 ttgagaatat ttttattgtg taacctattt tgaagcgtac ttaactagcc ccggccaaaa
    13681 tcaaattttt tcggggtata tcaagggttt gcgtcaataa cagaatgtta tttatctatc
    13741 acgttccttg gtggcgtgta gagttgatgg ttacgtagta gcgtatctac cagacgcgta
    13801 aattttcttg cggttaagac gtgtgctcgt ttgtgtttat ttttgggacc tctttacatt
    13861 tgctttgata aaaagcttta tattgaggaa gatatttttt tacagagttg gaagcttcaa
    13921 ctaagtagta tctaaaatag cgactccttt gttttgtcag aggtgtattt tgtgaccgat
    13981 agttaacaga ttgtttgcct ttccaacata agcctgcgta ttttgccaat tttgtttgat
    14041 cttcaaatcg ttcaatttga ccgatttcag cgattaatcc agcggcgtgt acctttccaa
    14101 tacctggaac actagtcaaa tattgatatt ctggaataac gacaacaaat tgctcggttg
    14161 ctttatccaa gtctttgatg ttcttttcaa gcgcacgaat ttcacataca aaaacattta
    14221 gaacgacatt gatggattct tatgcaagac tatctaaccg ataactcacg gcaattgcac
    14281 gttgaatcga tttggcattt ttttctggat ctttaaagcg acatttcctt tttcttttag
    14341 taagtcacag aaagcttcca aaggaagcac agccaattca tctagcgtga agtcttctgt
    14401 cacaagagta atgacagtgg cactatagag acttgtcgat tcatcgcgca tttatcgagt
    14461 aagcgtattg catttgtacg ttagattttc cagaaagtgt tgttttgtac gagtgaagtt
    14521 gcttaatgag tcgatagcga gtgcgtgtta aacgctgtaa agccacgtac ttctcttctt
    14581 tgataggaga agttgtgaac cgttgaatac gcaataaatc agcaattctt aacgcgttgt
    14641 ttcgatcagt cgtatcttcc tcaaacatgc gactgaattg tttcatccga aaaggatttt
    14701 caatcctcac aagtgcattg agttttttta gctctgcatc ttcatggaaa aaca
//
LOCUS       DAPBAB010000059        14269 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPBAB010000059 DAPBAB010000000
VERSION     DAPBAB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.062"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="RD444_002186"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="RD444_002186"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDT7549545.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="RD444_002187"
     CDS             complement(2262..2513)
                     /locus_tag="RD444_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDT7549546.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="RD444_002188"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="RD444_002188"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDT7549547.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="RD444_002189"
     CDS             3463..3681
                     /locus_tag="RD444_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549548.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="RD444_002190"
     CDS             complement(3970..4482)
                     /locus_tag="RD444_002190"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDT7549549.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="RD444_002191"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="RD444_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDT7549550.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="RD444_002192"
     CDS             complement(6935..7378)
                     /locus_tag="RD444_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDT7549551.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="RD444_002193"
     CDS             complement(7379..8167)
                     /locus_tag="RD444_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7549552.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="RD444_002194"
     CDS             complement(8164..9105)
                     /locus_tag="RD444_002194"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDT7549553.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="RD444_002195"
     CDS             complement(9282..9869)
                     /locus_tag="RD444_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDT7549554.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="RD444_002196"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="RD444_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDT7549555.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11700..12710
                     /gene="gap"
                     /locus_tag="RD444_002197"
     CDS             11700..12710
                     /gene="gap"
                     /locus_tag="RD444_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDT7549556.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12795..13730)
                     /locus_tag="RD444_002198"
     CDS             complement(12795..13730)
                     /locus_tag="RD444_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7549557.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13916..>14269
                     /locus_tag="RD444_002199"
     CDS             13916..>14269
                     /locus_tag="RD444_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549558.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFD"
BASE COUNT         3983 a         3167 c         2520 g         4599 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgtatta attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata
    11701 tgacagtaaa agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca
    11761 tcaaagaagt ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta
    11821 tgttagcaca attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg
    11881 caacagaaga ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg
    11941 caagcaaaat cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt
    12001 tttatacatc tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta
    12061 tttctgcccc agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag
    12121 atgaaaacga caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg
    12181 cttatttctt aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct
    12241 acacttccac acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc
    12301 gttcagctgc tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag
    12361 ttatccctga gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg
    12421 gttcattaac tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg
    12481 aagcaatcaa aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg
    12541 tttctagcga tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag
    12601 taacaactgc tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg
    12661 gatttacttg ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat
    12721 caatcgataa aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct
    12781 acttttaaac tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat
    12841 ttttcaggat ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct
    12901 tttggtccct tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct
    12961 tcgtctccat gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt
    13021 gcttcaccga aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc
    13081 ggaaatgctg gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa
    13141 gaataaccgc aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc
    13201 atcataactg ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca
    13261 ttgttttcta gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat
    13321 ccaatgtaat ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa
    13381 cccatatcat gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca
    13441 tgagccacaa gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat
    13501 ttcagaccgt cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat
    13561 ttatgtgcat ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag
    13621 tctttctcag aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca
    13681 acaatcagag ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca
    13741 tctcatttct cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc
    13801 aatgttcatt tttataaccc gcgaattgta aaattaagtt agaaaaataa aaaggcattt
    13861 atggtacact caaattgtat ttccgacgaa agaaaacaaa ggagtgtaac cataaatgac
    13921 ctacaaacat cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa
    13981 taagccggtt gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt
    14041 caataagttc aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa
    14101 gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga
    14161 gaaagtcact cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggtctgtttc
    14221 atgcggtatg agaacacttt atcgtttatt ttctaaagga atatttgat
//
LOCUS       DAPBAB010000060        14114 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPBAB010000060 DAPBAB010000000
VERSION     DAPBAB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.063"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..552)
                     /locus_tag="RD444_002200"
     CDS             complement(<1..552)
                     /locus_tag="RD444_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDT7549559.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
     gene            complement(568..1047)
                     /locus_tag="RD444_002201"
     CDS             complement(568..1047)
                     /locus_tag="RD444_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDT7549560.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1053..2077)
                     /locus_tag="RD444_002202"
                     /pseudo
     CDS             complement(1053..2077)
                     /locus_tag="RD444_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2094..3200)
                     /locus_tag="RD444_002203"
     CDS             complement(2094..3200)
                     /locus_tag="RD444_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDT7549561.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="RD444_002204"
                     /gene_synonym="adhC"
     CDS             complement(3429..6026)
                     /gene="adhE"
                     /locus_tag="RD444_002204"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDT7549562.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="RD444_002205"
     CDS             complement(6331..6711)
                     /gene="yajC"
                     /locus_tag="RD444_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDT7549563.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="RD444_002206"
     CDS             complement(6808..7953)
                     /gene="tgt"
                     /locus_tag="RD444_002206"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDT7549564.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8171..8869
                     /locus_tag="RD444_002207"
     CDS             8171..8869
                     /locus_tag="RD444_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDT7549565.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9037..11676)
                     /locus_tag="RD444_002208"
     CDS             complement(9037..11676)
                     /locus_tag="RD444_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7549566.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12295..13326)
                     /gene="queA"
                     /locus_tag="RD444_002209"
     CDS             complement(12295..13326)
                     /gene="queA"
                     /locus_tag="RD444_002209"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDT7549567.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13519..>14114
                     /locus_tag="RD444_002210"
     CDS             13519..>14114
                     /locus_tag="RD444_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7549568.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3957 a         3095 c         2542 g         4520 t
ORIGIN      
        1 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
       61 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
      121 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
      181 accaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
      241 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
      301 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
      361 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
      421 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
      481 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
      541 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
      601 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
      661 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
      721 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
      781 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
      841 tacggctaaa cgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
      901 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
      961 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
     1021 taataccttg tttaaaatca ttttcattac agctacgctc cttgctcttg gatcttttga
     1081 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
     1141 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
     1201 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
     1261 gcttcttcaa tggctttttc ctgttcatca gttagctgtt ctttgatggc atcattcatg
     1321 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc taccccgcca
     1381 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
     1441 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
     1501 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
     1561 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
     1621 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
     1681 atcaccttaa agaaatgttc ttcatcattg aatagataag gaacggaaaa aatcgaataa
     1741 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
     1801 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
     1861 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
     1921 actggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttacg tctgcatctg
     1981 cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga cctaggctaa
     2041 gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt cttttaacaa
     2101 taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata aggcattgct
     2161 gtgattccga tattccgata aaaatctaaa ctgtccccca ttcctgtccc gattgaaacg
     2221 ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc acgataagcg
     2281 acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat accgtaagca
     2341 aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa cgtatgccat
     2401 aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag tgtttcgatc
     2461 aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat ctctggaata
     2521 gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt atgatttcct
     2581 tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta tttgatatgg
     2641 atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag tttcccgatt
     2701 tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt cttattttcc
     2761 ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc attcatgacc
     2821 cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc ttcatacaag
     2881 taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc gccttcttcc
     2941 atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt tgtcatctgg
     3001 taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac tgtccagctt
     3061 ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc gtctttgatc
     3121 gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt aggtacaaaa
     3181 atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt ttatatcctt
     3241 aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac tgtctaaaat
     3301 tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa aaaaagaact
     3361 tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt ttctacgctt
     3421 tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga gatcaatggt
     3481 tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat acggtctacg
     3541 gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt gatgccgaca
     3601 gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc tgttgtttcg
     3661 cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg gaaatagtca
     3721 tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat aatatgcggt
     3781 aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat tttatgtgcg
     3841 actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat tgtagaagcg
     3901 ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc taaatagtca
     3961 aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc catgactgaa
     4021 acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt gacagatgct
     4081 ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa tgcataatct
     4141 gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg cgttacttca
     4201 gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg tttgtcgatc
     4261 ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa agatgtgtct
     4321 gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc gcctccaaga
     4381 gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta tacggtattt
     4441 gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt tttacgtcgt
     4501 gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg gtcacaaacg
     4561 atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt cttttcaaag
     4621 aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc tacagttttg
     4681 acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc ataagaaccg
     4741 catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc gccttctgca
     4801 gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt gacatgcaac
     4861 tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt caacatacct
     4921 tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag aactggagaa
     4981 agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc aatcagcatt
     5041 ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc agaatgtcca
     5101 acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc taatttttgc
     5161 aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt tactgctgca
     5221 tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc attatcaaat
     5281 gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt ttcaatataa
     5341 gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta agcagacttg
     5401 accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt cattagcata
     5461 gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg agctccggca
     5521 gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg ggcacttgga
     5581 tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt gaaaatcgtt
     5641 gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg ttcagcaatt
     5701 tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc atgtttgata
     5761 ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc gtaaacaccg
     5821 cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa agctgccatt
     5881 gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat ttctttcaat
     5941 gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat tgtcttcgtt
     6001 ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg ttatttgtta
     6061 aataattcac atgtatatca tacaatattt atttttctat gtaaacactt ttttgtgatt
     6121 tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg tatagaaaaa
     6181 tatataaagt tatttattat tatttatatg acacacccga ttattcaata ttgagcaata
     6241 tatcccatgc tcttatctct ttcttttttg aataaaaaat gaggtaggac atctttctgc
     6301 cctacctcgt ctttaaataa gctttgatac ttatttttct tccttctctt ctgtctcttc
     6361 cagcgtttct gttgtttcag tttttacttc aactgtctca gctggttctt ctgttgtttc
     6421 aacagtagtc gttgcatcat ttgttacgac agtccctggt ttgaccgttt tgattgctgc
     6481 acgatcgtac tcaaggaaga tcccttcgca gtctaaaaca actgtccgtt tgtcattgtc
     6541 gatttctgaa acaactccat gtaatccgcc gatcgttacg actgaatcac ctggtttcat
     6601 gctatctaac aagttttgac gttcttgctg ttgtttcttt tgcgaacgtg acataaaaaa
     6661 ccacattgct aatagcgcga taaacatcac aagcattggt aatgttccca tttttttcac
     6721 ctcactaatt ctgtatctac aaaaaacact ttagcagaaa aatgctgata acactagttt
     6781 tttacttaat ttttaaaaga ttttccttta gaagcttttt gcgttttctt tattgaaccc
     6841 gtattcttca aaaaaggctt ctctaaattc aagcaaatta tcatccatga ttgcttgacg
     6901 tacttgtttc attagattca acaagaagta taaattgtgg taagaagtca aacgaatccc
     6961 aaatgtttca tcacatttga tcaaatggcg gatataggca cgtgtatagt ttttacatgt
     7021 ataacaatca catttctcat caattggacg gaaatcatgg gcatatttcg catttttcac
     7081 tactagacgg ccttgagaag tcatacatgt gccatttcga gcgattcgag taggcaagac
     7141 acagtcaaac atgtctactc cacggataac accatcgatc aatgaatcag cagcaccgac
     7201 acccatcaag taacgtggtt tgttttcggg gatcaacggt gtagtgaatt ctaatacacg
     7261 attcatttct gatttaggtt ccccgacaga taacccgccg attgagtaac cagggaaatc
     7321 catcccgact agatctttcg cgctttgacg acgaagatct tcaaaccctg ctccttggat
     7381 gattccaaat aatccttgac ggtctggatt agcatgagct ttcaatcctc gttctgccca
     7441 tcgagacgta cgttctactg attttttcac gtagtcgtaa ctttcatcga atggtggaca
     7501 ttcgtcaaaa ctcatcatga tatcagagcc caatttattt tggatgttga ttgctttttc
     7561 tggtgataaa aacatacgcg aaccgttcaa gtgatttttg aaatgcacgc cttcttcttc
     7621 gatattccgc atatcactta atgaaaaaac ttgaaagccg cctgaatctg ttaaaatcgg
     7681 ttgatcccag ttcataaatt tatgcagtcc gccagcttct tctactaagt cttcaccagg
     7741 gcgtagccat aaatgatatg tattacttaa aataacgcca gcacccattt ctttcaactc
     7801 ttctggggac attgttttta ctgtagctaa tgttcctact ggcatgaaca ttggtgtggg
     7861 gaaagttccg tgtggcgtga tcaattcccc taaacgcgcg ccagtatgtt tttctttctt
     7921 gattaaacga tagcgaatgg caggttgcgt cattcatttc cctactttct atataaaata
     7981 tcttgacaac tcccaccatc ataatcgttt caagtgcttt ttgcaagttc cctcggcttc
     8041 cttctacttt atttaatatt ctttttttta tccttttttt atgaataaca aaaaaaagaa
     8101 aaacattatg acagaaatat tttttgatac atggtatatt ggtgttatta aagaaaagga
     8161 gggaaaaatc atgcaatctt ctgcagaatt aaaacgccaa gcaaaagaga gtctaaaagg
     8221 acgttgggga caagctgttt tactaaatct aatccctaca ttgattggta ttgctactat
     8281 tcttcttatt atgattccta ttatcttgtt tgccaccacc atatatgatg gatcagcgag
     8341 cgtctcagaa gcgacaactt caggaggcgg tagttcagga ggcggtggcg gaattatttc
     8401 cagcatcatt agcgctttgt tcatgagcgg tatttcttgg acttatcttg atattttacg
     8461 tggacaaaaa gatcagatcg aaccattcaa agatgcattt cgtggattca aaggtttgtt
     8521 tatcggcgga gtgatcagct tagctgtgct gatctcgatc tttacaacat tatggggttt
     8581 gctattagtt attcctggaa tcattaaaag ctattcttat tctcaaagct attttattta
     8641 ctacgatatc gtccaagaaa caggacaaac acctaaagta ctggatacga tcactgcaag
     8701 tcgaaaattg atggatggtt ataaaggaca acttttctgg ttagatcttt catttatcgg
     8761 ctggcatatc ttagcattag caactgctgg tattggttat ctatggctca gcccatacat
     8821 ttctgcaaca aaagcagcat tttatgaaca attgccaaaa gatatttaaa agagagtgtg
     8881 acttgtgacc gagggggtgg caggagaaca ccgtttatcg agaggcagta aaagaaacat
     8941 tcagcccatt agcaaaaagg aggtgtgaca acattcatgt cacacctcct ttttgtatag
     9001 ttccagtaaa aagaaaatca acttagaaac agttaatcat tcctcttctt ctgcttccgg
     9061 taatcgatgg aacttgatca gttcattcac cgcgattggt acgatagata aaatccctac
     9121 caagaaccag tgattcaaac tgatgtgtac taggccgaat agttcttgcg taaatggcag
     9181 caaagcaaca gccgttgtaa tagctaatgc cagtaacgcc atttctacca aagctttatt
     9241 ggtcgctaaa ttgactttga agatcgattg gctgctgcgt acattgaaca catgcagaat
     9301 cgatgaataa gctaagatca agaatgcaac agtttgaccg acatgctgac ttgcgtctac
     9361 ccatgggctg atagtatcga cataactacc taaataataa ccaaacaagg ttacgatggt
     9421 gaagacccct gcatttatgg caattttttg caataaacct cgagcaaaga tcccttcatt
     9481 tttcggaatg ggtggctgat ccatgatgcc gtgttccgct ggctctcttc ctaaagcaaa
     9541 tcctggaagt ccatcagcaa ctacattgac aaacagtagt tgcacagctg taaatggtgc
     9601 tccccatcct aacagcatcg caatcaaaac aataaagatc tccgaaatat tacagcttag
     9661 caaaaagttg atagctttac gaatattctg ataaaccgaa cgtccttgac tgacagcgtc
     9721 tacgatcgtc gcaaagttgt catccgtcaa aatcatatct gcggcacttt gcgccacatc
     9781 tgttcctgtg atccccatgg cacacccaac gttacttgct ttcaacgctg gtgcatcatt
     9841 cacaccatcc ccagtcatcg ctacgactgc acccgtacgt tgccaagatt tgacgatccg
     9901 gatcttgtct tctggcgtta cccgagcata gacagagaga gctttgacac gcgcatctaa
     9961 ttcttcgtct gataattgat gcagttctga tcctgaaagt gcttctgacg gatcattcaa
    10021 gatacctaat tctttcgcta tggcactggc agtcaccaca tgatctccag tgatcatgac
    10081 tgttttgatg ccagcctttt tcgctcgctt gattgctcct ttactttctg gacgtggtgg
    10141 atcgatcatt ccgatcagac caatcaaacg taagtctttt tccagtgctt cagaagaaat
    10201 atcttgcggt tcttcgtcat aagtagcata accaacagca atcactctca gtgcccgttt
    10261 accaaagcga tcattaacga tcgccgcttg ttctacatcg cctgaactaa agcgcggagc
    10321 aagaacatcg aatgctcctt tggtgattga gatgtatttt tcgcccattt ggtgaactgt
    10381 tgtcatcatt tttcgttcag aatcaaaagg aagctcgttt actcgtggat atttttcttc
    10441 taattctgcc tttgtatgat aattctcttc aacagccctt acgatggcta cttctgttgg
    10501 attacctttg ataacaaggt cgtcatcttc ttgctcgacc gtcacatctg tacatagtgc
    10561 agccattttc aatatttcca tcgcgctgtt cgtcattgaa tcttcgatat tcgttacttc
    10621 atcttctttg ctccataccc ggcggacgcg catttcattt tgtgtcagcg ttcctgtttt
    10681 gtctgagcag ataacattag cggttcctag cgtttcaact gctggcaact gtcggatgat
    10741 cgcatgtttc ctcgccatct tctgcacacc gtatgcaagt gtcaacgtaa caataacagt
    10801 caaggtttcc ggtacagctg caacagccaa ggaaatcgaa gtcatgaaca tctcaaggag
    10861 tggttctcct tgcagttcac caatcacaaa tactaaagcc gcagccgcta aagcgatcat
    10921 actgattcgt ttgcctagct gattcaatcg gacctgtaaa ggtgtttttt gcatagtatt
    10981 ttcgttcaaa agatccgcga tcttacccat ttctgtagcc atgccaatgg cagtgacgat
    11041 cgctttccca cgacctgcag caaccgtaca tcctttaaat accatattaa gttggtcacc
    11101 taatgactca tcttcttcag caataaatgc tgcatctttt tcaactgctt cactttctcc
    11161 agtcaacgcc gattcttcta cacgtaattg tgatgcttcg atcaaacggg catcggcaga
    11221 aatagcatca ccggactcta aaacaagaat atcacccact accagttgct ctgactcaac
    11281 agtggtcact ttgccgtctc gtatgacagt tgccgtttgt ttattcatat cttgcaagga
    11341 ttccagtgct ttttctgcgt ttccttcttg gacaatcgcc aataccgagt tgatcacaac
    11401 gatagcgatg atcagcagtc cttcaaataa atccccatgt tctgtagcaa atgcggtata
    11461 aaaggaaatc accgctgcta caagaaggat gatcgttgta aaatcggaca agcttcgaaa
    11521 gaattttttg atcatcgatt ctttcggcgc ctcatcgaat ttattcaatc cctgcttttt
    11581 tcgccgttca tccacttctt gtgaagacaa cccagtatct ggatctgctt ctgttacctt
    11641 ctttacttct tctattgtct gcttgtattc ttccatccta ttcctccttt aaaatcttcg
    11701 tacaaaaaaa ggtacttatt ttactataac atcctttttt tatttacttc aaataaaggg
    11761 cttatcgatt atttgttaag aaagcgtaaa atttaaagtg cttatatttg caaacgctac
    11821 caaaggatgc tattatatag atgtaaggaa ggaggatacc atcaaataga cagaagaatt
    11881 tctcgataga acagtgtaag caaacaaagt tgttagaagt acaggtttca cccagacatg
    11941 cgtgtagagt acaaacctat aaatagtgaa tgcgtatgga tgtttggatc acgaagtatc
    12001 cgaaacaaga agtaatgcga tacgtaacaa ttgaataacg taaacgtctg aaaattgaat
    12061 aggaacaaga gatgtttcaa atgttgaaga tgtaacgaaa acaaaatttt tgtttaacat
    12121 gaagaaattc aacaccgtgc tttgcatgta tgatggaaag ttacaatcat ttctgtttat
    12181 ttgaatcctt ccgaggtaca agaaagccga gcttatcgag agctcggctt tttctttgtc
    12241 ataaaatctt tgtaaagctt ttttgttatt caaaactagc ggcttttcat aaccttattt
    12301 cacgaacatc gcatctccaa aactaaagaa acgatagcgc tcttcaatcg catgctggta
    12361 agcagataaa gtcaattctc ttcctgcaaa ggcactaact aacatgacta aagtcgattt
    12421 tggcaaatgg aaattagttg agaaagcttc taccactttg aattcatagc caggcgtaat
    12481 gaaaatatca gtccatccgc tgtctgcttt gatttctcca ttaaatttcg ttccaatcgt
    12541 ttctaatgtt cgaattgaag ttgttccgac agccacgatt ttcccgccat ttttacgtac
    12601 ttggttcaat cgctcagccg cttcttccgt caaacggtag aattcactat gcatatggtg
    12661 ttcttcaatt ttatctacgc tgacaggtcg gaatgtacct aaccctacat gtagtgtcag
    12721 atagactaat tcaacaccct ttgcttcgat ctcatcaagt aattctttcg taaaatgcaa
    12781 tccagctgtt ggcgctgcag cagaaccatt ttctttcgca tagacagttt gatagcgatc
    12841 tgggtcttcc agacgttctt tgatatatgg aggcaatggc atttctccca atgattcaag
    12901 aatctctaga aaaattcctt catactcaaa gtcaatgatc cgtccgccgt gttccaattc
    12961 ttctttcact actgcttgca atctgccatc accaaaagag attcttgtcc caactttggc
    13021 acgtttcgca ggtttgatca atgtctccca cgtatctcct tgtgtattcg ttagaagaag
    13081 gacttctaaa tgcgcaccag tatctggttt ttctccatat aaacgagcag gcagaactcg
    13141 tgtattattc ataactagcg catcaccagg ctgtaactct tcaatgattt catggaaatg
    13201 tttatcttcc atctctccag ttttccgatc caaaacaagt aaacgagaat gatcgcggtc
    13261 ttttaatggt gtttgtgcga ttaattcttc cggcaagtca aaatcaaaat cttctgttgt
    13321 taacatatcg tcactcactt tctatcatgt gcttcatcat tctatcatgt ttttcgttct
    13381 gttggcaaaa tcagcacttt ttcacttcta ttttttgttt tcctaaataa aaaaactacc
    13441 ctatttgtat tttttcattg ttttaggcat actagtaata gatagttctt cattgaaaat
    13501 agaaaaagag gtggcaccat gattgaattt caaaatgtgt caaaaattta taaaggtgga
    13561 aaagtcgctg tagaggacgt caatctttca tttgaaaagg gtgagttcat ttgttttatc
    13621 ggtacgagcg gaagcggaaa aacgacgacg atgcggatga tcaaccgaat gactgatcca
    13681 acacaaggga aaattttgat tgatggaaaa tccatccaag aaattgatcc agtggaattg
    13741 cgacgtcaga tcggctatgt tatccaaagt attggattga tgcctcacat gacgatccgt
    13801 gaaaatatca ctcttgtcca aaaattgttg aaagtcagtc aagaagaacg gaataaaaca
    13861 gctgaaaaaa tgattgatct tgtggaattg ccccgtgaga tgttagatcg ctacccacat
    13921 gaattatctg gtggtcagca gcagcgtatt ggagtcgttc gtgcccttgc agcgaatcaa
    13981 gatatcatct tgatggacga gccattcggt gcgcttgatc caatcactcg tgattccttg
    14041 caagaccttg tcaaagattt acaagagcgt ttgggaaaaa cgatcgtatt tgtaacccat
    14101 gacatggatg aagc
//
LOCUS       DAPBAB010000061        13980 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPBAB010000061 DAPBAB010000000
VERSION     DAPBAB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.064"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(142..1086)
                     /locus_tag="RD444_002211"
     CDS             complement(142..1086)
                     /locus_tag="RD444_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDT7549569.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1220..2611)
                     /locus_tag="RD444_002212"
     CDS             complement(1220..2611)
                     /locus_tag="RD444_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDT7549570.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2737..3147
                     /gene="tagD"
                     /locus_tag="RD444_002213"
     CDS             2737..3147
                     /gene="tagD"
                     /locus_tag="RD444_002213"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDT7549571.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3323..3736
                     /locus_tag="RD444_002214"
     CDS             3323..3736
                     /locus_tag="RD444_002214"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7549572.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3893..4150
                     /locus_tag="RD444_002215"
     CDS             3893..4150
                     /locus_tag="RD444_002215"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7549573.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4237..5475
                     /gene="istA"
                     /locus_tag="RD444_002216"
     CDS             4237..5475
                     /gene="istA"
                     /locus_tag="RD444_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDT7549574.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            5465..6205
                     /gene="istB"
                     /locus_tag="RD444_002217"
     CDS             5465..6205
                     /gene="istB"
                     /locus_tag="RD444_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDT7549575.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            6615..6779
                     /locus_tag="RD444_002218"
                     /pseudo
     CDS             6615..6779
                     /locus_tag="RD444_002218"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6846..8273)
                     /locus_tag="RD444_002219"
     CDS             complement(6846..8273)
                     /locus_tag="RD444_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDT7549576.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(8270..9412)
                     /locus_tag="RD444_002220"
     CDS             complement(8270..9412)
                     /locus_tag="RD444_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDT7549577.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            complement(9416..10411)
                     /locus_tag="RD444_002221"
     CDS             complement(9416..10411)
                     /locus_tag="RD444_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7549578.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            complement(10525..11514)
                     /locus_tag="RD444_002222"
     CDS             complement(10525..11514)
                     /locus_tag="RD444_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7549579.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            complement(11486..12316)
                     /locus_tag="RD444_002223"
     CDS             complement(11486..12316)
                     /locus_tag="RD444_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7549580.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            complement(12354..13745)
                     /locus_tag="RD444_002224"
     CDS             complement(12354..13745)
                     /locus_tag="RD444_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDT7549581.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
BASE COUNT         4732 a         2537 c         2073 g         4638 t
ORIGIN      
        1 aagttctctt tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa
       61 gaattaaata gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc
      121 aaagatgcaa tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa
      181 tatatcaaat ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc
      241 acgaatatcc cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg
      301 atcaattgaa tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg
      361 ctgtcctaaa gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat
      421 ataaacaaag tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc
      481 tgccaattgt tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt
      541 tggattttga tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata
      601 taaatgacag taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt
      661 taatggacgg atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc
      721 agctgatgaa ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag
      781 caataaaagc gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc
      841 tgccacacta gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat
      901 tctttccatc aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat
      961 atccaagttt tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt
     1021 tcccaaataa ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt
     1081 gtccatcaaa ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt
     1141 tatattgtaa aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt
     1201 catataccta aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga
     1261 tagaacccag atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg
     1321 tgataaataa tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat
     1381 cttttactag atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata
     1441 agaaggttaa caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa
     1501 aattatgagc agttaaagta tcttttccca ttaatgtata tggtagttta tcatgtgcat
     1561 attcaatata gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg
     1621 cacttttcca aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt
     1681 ttaaatcaac tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt
     1741 ctgtaatatg cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg
     1801 caattatact tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt
     1861 taagaaaacg actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac
     1921 aaactaatgt ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa
     1981 ttacaaaaaa tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag
     2041 caataatgat gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa
     2101 ataaacggtt gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt
     2161 ttatgccata ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca
     2221 atgaaagtat ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact
     2281 gataaatcat aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc
     2341 catattttaa attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc
     2401 aatcatattt ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga
     2461 caactcccgc tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta
     2521 aatacgggcc tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt
     2581 tcacatattt taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa
     2641 tatttcttat cctttttagg ataaactggt cctttcaatt ctaacaaaaa aatgctaaac
     2701 taacaatgta tattaatgaa tttcgagagg tgaaagatga aaagagtaat tacttacggt
     2761 acctttgatt tgcttcatta cgggcatatc aatttactga aacgagcaaa acaatacgga
     2821 gactatctta ttgttgccct ttctacagat gaattcaatt ggaatgcgaa acaaaaaaaa
     2881 tgttatttta gttatgaaaa aagaaaacag ttacttgaag ctattcgcta cgttgattta
     2941 gttattcctg aagaaaattg ggaacaaaaa gtaaaagata taaaagaata ccatgcagat
     3001 tatttcgtga tgggcgatga ttgggctgga cattttgatg aacttgaaca actaacagat
     3061 gcacaagtga tttatttacc aagaacacct gagatctcta caactcaaat aaagaacgaa
     3121 cttaattctt taaatagtga taaataatat tttttaaaca ataaaagaac aatatatctg
     3181 gataagacgt aaaaaaatgg ctctataatt tctaggactg atagaatttt ctgtcggtcc
     3241 tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga gtcgtctaaa
     3301 acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag agctcttcac
     3361 tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata aggacaagaa
     3421 agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat tttgtaaaga
     3481 atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct taaatcgcag
     3541 tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc aatcatgcgg
     3601 gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt cgaaacaagt
     3661 tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg
     3721 ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc aacgctgcga
     3781 cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt
     3841 ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct ctttgacatt
     3901 ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc
     3961 tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt cattagtcaa
     4021 agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc aacacttgaa
     4081 caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg
     4141 gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta catttttgta
     4201 tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat acgcgaagga
     4261 gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc aaaacaatac
     4321 gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa agaatcaaaa
     4381 cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat catccaagac
     4441 aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca caaaggattt
     4501 ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc gaaagtacac
     4561 aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga ctggaaggaa
     4621 gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt tttgtacgtc
     4681 ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca agacacactc
     4741 tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga aatctggttc
     4801 gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc gcatttcaac
     4861 aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg tcggccattt
     4921 cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc
     4981 tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt
     5041 gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg ttggcagacg
     5101 aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta ttttgaagac
     5161 tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg caggtactct
     5221 gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa agaagaccaa
     5281 gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac aaaacagctg
     5341 aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa
     5401 gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct ggtggaggtt
     5461 gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc ttggtttgaa
     5521 tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg agctttcttt
     5581 aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata agcaaaaaat
     5641 gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag agtttgattt
     5701 tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca tgggctttgt
     5761 ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga cacatctgac
     5821 gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca tcacctgtca
     5881 tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc gtgttctcaa
     5941 acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc cgattcaaaa
     6001 acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc gctctacgat
     6061 cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt ctgagatcgc
     6121 agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca caggcaagtc
     6181 ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat tgccgaaaac
     6241 ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat caaaaagaca
     6301 cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt ctataatcag
     6361 atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg cgctatttaa
     6421 cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa atggcttagc
     6481 agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact tcgagaaatg
     6541 accaaagaag ttccctcgaa acttcactat cttaaaaagc ctttttcata tctgaaaaag
     6601 gcttttaact agcattttcg aaaaagtatt ttaatacaaa attagagaat cttcgcacac
     6661 atatcaaaac attgaaacga tttgcttatg gattttgcag ttttaccaat atgcgcttgt
     6721 ggatttttct aatcaacggg ttaataagct atgcattaaa agctagtaaa tcttactaac
     6781 tttgttgatc ataaatgacc ttatcagccc gatttgacaa agaactactt ttttacaaca
     6841 ttattttatt ttcgaataat ctttcttact tcatttcgta tatctggaaa aagaaaataa
     6901 acaaacggaa gataaataat gaaaatcatt attgatttaa ttgccatatc taaaaatgaa
     6961 gaagagaata attgtggaag aaagtaatag gccgaagtag tcaaagtaag attgactata
     7021 aaaaataaga caacgttctt cccaaagaaa taggatattt ttatttcttt tcgtacaaaa
     7081 aatactctaa agaaaaagac taataattct actaccacga gtacgattgt agccgctaca
     7141 gcgccataag taggcacaaa agctacattt ccaataatac ttacaatcgc accaatgatc
     7201 attggataag catactcctt atctttttct atagcaattg caaattggtt agcaaaaact
     7261 ccaccaattg gactaacaac tataattaga caagaaataa gcatgtttat tgtcatcggt
     7321 ttgaaattct ccccaaaaaa ccatggaaca aattcttttg tattgataaa caccacacta
     7381 aaaagaatta atgcaataat aatcgtgtaa tcaaaagatt tttttgtgta aactaaaata
     7441 ttttctgatt ttccctctgc ctgtgcttta gatattttag gcaacataac aatagtaatt
     7501 gatgttaaga ctgccaatat aatacgagca attttttgag actgatcata ataagataat
     7561 gctgttggat ccacaaaata cccaactatc gttttgtcta aagttgtgta cacttgcata
     7621 aacaactgag gagtcaaaat aataaaagct ggtttccaca actgtttaaa aacattagag
     7681 ttaaagagtg gtttacctac ttttccgtgg atactcaacc agaaaactaa acttcctaaa
     7741 aagttagtaa ttgaagtaat gaacacataa atccacaaat catctttagt tgaaacaaaa
     7801 aggattatta acaataccga tgataatttg ataactgtat ttctaataac cactttcgaa
     7861 aattcttcaa ttccttgaaa aaaccaagaa atatctaatg ttagccctgc aataaatatc
     7921 aattgaatta agtataaatt tttatacttt acatcaaaaa aataaatata ggctatatag
     7981 agaaaggaaa cactcaagcc tattaaaaat tgaagaaagt aaatattcca gaattgtaaa
     8041 gatagtgctt ttttatcttt ccttgattta gcgatatttc gaatacgaaa aggattcatt
     8101 cccacagcaa tgacagtcat taaaaattgt gatattgaga aaacataccc ataaatccct
     8161 aaaccagttg aacctattct acgactaagt ataggtatag taagtaaggg caaaacaatc
     8221 aaaaagactt gatatatcgc attataaaat atatctttta ctgttttttt cactttatat
     8281 ccctctctaa caactcaaga accttttctg tacttgcccc tgtttcataa tttccaatat
     8341 aatcaagtaa ctcttgagaa cttttcaaaa gcttttcttg ttggttttta aaaacatcaa
     8401 caaggccata actatcttgt acgcttataa ttggtaactc acttaactgt atataaaaac
     8461 ctctttctaa gctatattct ttttcatcat agttaaaaag aataattggt ttttttgtta
     8521 gcgcaaaatc gaaaaaaata gatgaataat ctgtgattaa aacatctatc tcttcaaaaa
     8581 gcatttcaag aggaatccca tcattaatta aagtaatttg atgttttttc atatattcta
     8641 tacttttaga atcagttgct aatttactat gaacattagg atgaaattta gtcattaaat
     8701 ttaattcttg ttgttgcttt ttaaaaaatt cacgtaagtt atccacacct aaccattcta
     8761 gttgcgtaat tccgttgtcc tcaatattat ttcgaaaagt tggagcaaat aagacattga
     8821 tttgtttctt tttcaagtca aacacttgct ctttactaga ggctaattca tcgtttctag
     8881 gagttcctac ttctaatatt ttagaaggaa cgatattgaa tgctgaaaca tacttctcag
     8941 aactatagcg attaccagat aataaatatg taattgactc actatctttt ttcgcatagc
     9001 ttaggtaacg tttaggtaac ttatcttttg catcaaattc aatttttttt aaaggcgtac
     9061 cgtgccatgt ttgaaaataa aattgttttt tcctttttgc atacttaatc gttttccgac
     9121 tatcgtctat ccaatactta gctgtataca aataaaaggc agctaaaaaa gaatcggaat
     9181 caattctttt aatataatct ggtaaatcag ttataatcgc ttcttttttg attaaccaaa
     9241 taattctgta attcaatttt ttttcatgaa ttttctctga aataactctt ggatttccag
     9301 aataattacg cccgccaaaa cttgaaaata caattaaatt tttttgaata ggtaatattt
     9361 taataaaaac cgggaaaata ttccttgaaa aaaatttttt tatattcttc atctcttacc
     9421 tcacagattc tatcttaatc aaaaatttac ttgctatctt actacttgac atatagtaag
     9481 gcaagttttt atttattaat aaaaacttta ctaatttttt taattttggt gaaaagtcgg
     9541 gatcaatttt cacttctttt tcaaagcgtt gtaaaatatt tatagaacgt aaataatctt
     9601 ctttattggt atagttattt ttaatcattg tcatcatcat ccttattgct tcatagacat
     9661 agcgagttct aacattaatt gataattccg gataaaactt atctatgaat actagtatat
     9721 cttcatggac ttcaaaagaa tcaaaaattc taggattgaa gcttcctctc agtatacttt
     9781 tagggctttg gtaatagtga tatttagcag atgaattgac agcaactttt tcggaatgat
     9841 cgatcaacaa ataagtagta gccttatctt ccattaattt acccagtggg aatcttatat
     9901 tatcaaataa atttctattg aataatttat tatttgcaga atagccaatt ttattattta
     9961 taaataagtt tttcattgct tgttctctat caaatatttc ccattttctc tcagcaattg
    10021 tatgttcttt tagttgttct aaacttgaaa tcttttttat ttttagcacc gagatatctg
    10081 ctccataagt ttgcgtacca aaatatagta actcaagata gtctggttct atccaatcat
    10141 cgctgtcaat aaagcacaca aaatctcctg ttgcagaatc aagaccagaa ttcctagccg
    10201 cacttaaacc accattcact tgatgtatca cttttaatcg catatctttc tttttatatt
    10261 cttctgcaat ttccccactt cgatccgggg aaccatcatc gactactatt atttcaatat
    10321 ttgaatgtgt ctgattggaa atagattcta agcagttttc taaatagttt tctacgttat
    10381 atgcgggtac tataacacta attttatgca ctctcttcac ttcctaaaaa tcatcttttt
    10441 tatttttaca aatgtatcat taccagtaac aattttagtc ttccgaagtt aataaacata
    10501 aaaaataaca tatcgaaaaa aactttattt ttttagaact tttttatata aaataaaata
    10561 agttttagga ttaattaagt ataaaaataa agctaatttt ttagcaattg atatctcatt
    10621 gttacttctt atttttgaat atatactttt tatttctctt ttagactgat tataatattt
    10681 ttgtttttct ttctgatttt gatttttgcc agtaatttta aaccctgttg aaagatatcc
    10741 gtctactaac cgagcattgg ccaaattaat aagtcgagga taatctttct ttacttcatc
    10801 aactaatatt tttctctgca aaatattatc atactgtttt gaattaaaag actgataaac
    10861 gatgctatcg ttattttgat aataaaaaat acctgaaaat ggcaaaatat caattttact
    10921 agccgcccta aaagcttcat acataggaac catatcctca tataatttat tttctggaaa
    10981 aactatatga ttgaataatt cccttttaaa aaatttattc catacgtaaa agtccactgc
    11041 cttacttcta aacatttgct caattgcttc ttcacttgaa caaccaatct tcttttcggg
    11101 aacaaataaa tttgcatttt ctatattttt tcctttactt aatccaaaag caatgatatc
    11161 ggggtgattc ctatggtagt ctttcaaaac attattaaaa caatcaggta aaaaataatc
    11221 gtcactatct aaaaatccta ccaaatttcc agttgcttct tttatcccat agttcctagc
    11281 aactgatgga ccagcgtttt ccaagtgata acatgctact ttactctcta gattagcaat
    11341 ttcgtcacca atgtcactac taccatctgt agatccatca tcaattaaca agatttcaat
    11401 attgggataa ccttgcgtta agacgctgtc tacagctctt cttaaagtat tctttgcatt
    11461 gtaaaaaggt atgattatag aaagtttagt ttgtttttct aagttttgat tcatatactt
    11521 ttcccctaat tttattcggt aataaactaa ttacaagatg tccgcaaata actatcagaa
    11581 aatcttttag tgatcctaga ccatctttcc atacgcgata tcggccttta attcctggta
    11641 aaagatattt acttcctcca cgtctagcat acatgtcagc acctgctcta gcatgtacta
    11701 gaatctcttg gatattatat cctttatatc ccgccttcaa tagtcttacc cacaaataat
    11761 aatcttcaaa tccaattaat gggcgatagt ttcccacttc taaaatagca tttttatcaa
    11821 acatgactgt catatgatta aaagggtttc ttcttttcga aaacacacgg atttcatcat
    11881 tatactcagg tacaatcctt cttccgataa cattgctaag tgtgccagta aattcttcaa
    11941 tattactccc aacaatcgct atttctggat attcttggaa gatctctaat tgtttttcca
    12001 atctgtgtgg ttccatgatg tcatctgtat ccattctggc cactaagcga tttttagtat
    12061 gttctaatcc agttgctaaa gcaatcccca atccttgatt tttttctaat tttacaactg
    12121 taaacaagtg aggaactttt tgctggaaat aagtgacaac tgcctgaagc ttctcattta
    12181 tagggccatc ataaaccaat acgatttctg ccggcatcac cgtttgctca aagatactag
    12241 ctaatgcttc ctttaaatat tccggctttt ctttatcata caaagaaagc agtatactta
    12301 tatttttatt ttccaatgtt ctctcctact tctttgctca aaatttttcc aatctaacga
    12361 tacgtttttt cttttggaaa tgcactttct gctaatgaac tgatattttc atcttcatct
    12421 agtccttgtg aggacatctt atcaaataag attttgattg tttgaagtaa aatcttcaaa
    12481 tctaatagta aggaatactt tttgatatac aacaaatcaa aacgtagttt actgttataa
    12541 tctgatacat attttccgta tacttgtgca tatccggtaa ttcccgcacg tacattgtgt
    12601 cttaagtaat aataaggatt ttcttcattg aactgatcaa cgaaaaatgg tctttctggc
    12661 cttggaccga caatcgacat atcgcctttc aatacattaa agatttgagg taattcgtca
    12721 aaacgtacag cacggataaa tttacctact ggtgtcacac gtgcatcgtt acttttagcc
    12781 aagactggtc ctgattttgc ttcagctgtc gcactcatgg aacggaattt atagattgaa
    12841 aattcctttt gatttttcgt aatacgtact tgtttataga tgattggtcc aggtgaagtt
    12901 gtttttacca aaatcgctgt gattagcata aaaggtgagg cgaggatcag taaaataagc
    12961 gaaacgatga tatcaaagag acgcttgaat agttcgtatt ctggttggat tctgaaccca
    13021 gaagcttcaa tgatgctttc atcttcaaaa ttcataatat tcggatttat cattgtcaga
    13081 ttctcaaaag tcgtatttaa aaataatttc ttctcttttt tagtcactaa ttgataaatg
    13141 ttgatttttt catcttctgg gattttagaa gccaaataga cgatatccat atcatcgatc
    13201 aatttttttg tattttcgta ataattatcg attgccactg cttcaacctt atgtttattg
    13261 tttttcttag attgaaaatt ttggatagct ggtgcgacat ctttctccaa acccacgata
    13321 aggactttct tatcacgact gactcttaag tacagtttgt aaacgactat ccggtaaaca
    13381 ctaagtaata tcgtacctag gataaacgag atcaaaatga cgccacgcgg aaatgcaaac
    13441 catcggccag caaaagtaac taccatgatc cccaatgcta ttagtacttg tccaataaca
    13501 gtaatgaaga caatatcact gatcattcgg ttatagaata cataagctcc taacaaaata
    13561 ttcaacgcaa taaagatgat ggaaataaaa attgctgaat gctgaaaggt ttctaagttt
    13621 ctcactggta cttctccctg gaatttcagc aaaaaactaa gatagatcga tacatttaat
    13681 acaagtacat ccataagaat aatgaatatt ctgcgtgccg cattccactc cccattttta
    13741 ttcatatata cactcctaac cataaaataa tgcctctatc atcttaccat agcaaagttt
    13801 aaaaacctag tgaataacca aattgtaaag gaaattaaaa gaaacgggga aaagcgttta
    13861 ttgtctgttt atatgtgtat ttctgcataa cagaaagttg tctcagaagt cccatcactt
    13921 cctttatgct gaactagtat tctcctgcta attccttatt cacaagttac aatattcata
//
LOCUS       DAPBAB010000062        13338 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPBAB010000062 DAPBAB010000000
VERSION     DAPBAB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.065"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="RD444_002225"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="RD444_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDT7549582.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="RD444_002226"
     CDS             complement(740..1462)
                     /locus_tag="RD444_002226"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDT7549583.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="RD444_002227"
     CDS             complement(1598..2479)
                     /locus_tag="RD444_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDT7549584.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="RD444_002228"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="RD444_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDT7549585.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="RD444_002229"
     CDS             3719..4198
                     /locus_tag="RD444_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDT7549586.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="RD444_002230"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="RD444_002230"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDT7549587.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="RD444_002231"
     CDS             5499..5627
                     /locus_tag="RD444_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDT7549588.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5631..6176
                     /locus_tag="RD444_002232"
     CDS             5631..6176
                     /locus_tag="RD444_002232"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDT7549589.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6631..8322)
                     /locus_tag="RD444_002233"
     CDS             complement(6631..8322)
                     /locus_tag="RD444_002233"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDT7549590.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8742..9689)
                     /gene="arcC"
                     /locus_tag="RD444_002234"
     CDS             complement(8742..9689)
                     /gene="arcC"
                     /locus_tag="RD444_002234"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDT7549591.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9804..10823)
                     /gene="argF"
                     /locus_tag="RD444_002235"
     CDS             complement(9804..10823)
                     /gene="argF"
                     /locus_tag="RD444_002235"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDT7549592.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10914..12143)
                     /gene="arcA"
                     /locus_tag="RD444_002236"
     CDS             complement(10914..12143)
                     /gene="arcA"
                     /locus_tag="RD444_002236"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDT7549593.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12604..13305
                     /locus_tag="RD444_002237"
     CDS             12604..13305
                     /locus_tag="RD444_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7549594.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3754 a         2711 c         2368 g         4505 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa aaagggagct gtgacacgac ttttgtcaca gtccccttat ttgcaaacaa
     6481 acagtgttta agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt
     6541 tgctcaaaaa acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga
     6601 tcagaacgct gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag
     6661 caaacgaagg tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc
     6721 tttttgttca tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc
     6781 taatttgatt gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac
     6841 cgtttcaggg tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc
     6901 atagtctgct gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc
     6961 atgtgtatat tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt
     7021 aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc
     7081 aacttgttta gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc
     7141 tttagcagat tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg
     7201 agttgataat tttttaccac cttgcgtgat caaaccaaat ggaatatggt gcatgtcgtc
     7261 agaccagtca aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg
     7321 ttcattccct actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc
     7381 agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg
     7441 attcaggtca taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg
     7501 tttttcttct aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt
     7561 caacgaatcg aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc
     7621 atcacggaac cattgccata attgaatagc ctctttatcg ccttcttcca gacgtttgaa
     7681 ccaagcacga gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac
     7741 gtataaacga agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt
     7801 tttgtaagcg acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg
     7861 gattggctga taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga
     7921 acgcaaatgt cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac
     7981 tgttccttga ttcccgatgt tgctatcccc ataatgttct ttttctttca ctacggtttg
     8041 caaaactttt ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac
     8101 ttcgattttt tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg
     8161 tggcgctttt cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc
     8221 agctgatttt ggattttcta gtaattgctc gacttgttcc agtgttaaat catctttgac
     8281 agcgtcatag acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt
     8341 gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac
     8401 tagagacgag atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct
     8461 gatatcggta gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct
     8521 tcttcattcg gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct
     8581 tgttcaacaa atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa
     8641 ggctagtcgt ttcataaaaa aaaaatccag atggcctaaa ggagaagaaa taccacctgg
     8701 atagctataa atcatttcgt aaatacgaaa gacttaacaa attaatcagc tacgataatc
     8761 gttccttctt cagaagctaa aagattctcg atattttcta aagaagtgat gattgcctta
     8821 gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta ctttcggaag catgcttcct
     8881 ggagcgaatt gcttttcatc aatgtattgt ttcatttctg aaacagtaac agtttctaat
     8941 ttcttttgat ctggtttttg gtaattgaca tacacattgt ctactcctgt caatacgatc
     9001 agcaaatccg catcgatgat ttcggctaat ttttctgaag caaaatcttt atcgattacg
     9061 gcttctcgtc cttctaatcc agtagctgtt tctacaacag gaatcccacc gccaccaaca
     9121 gagactgtga taactccttg atcgaccagc gtttcgatga cacgtgcttc tttaatcgag
     9181 atgggtttag gggaagcgac tacttttctc catccacgtc cagcatcttc tttgaatgtc
     9241 acagtggagt cagcattcat ttgttctttg gcttcttctt ccgtgtagaa tggaccgact
     9301 ggtttacttg gatttttgaa tgaaggatca ttttcatcca caataacttg agtgactaaa
     9361 gaaaccacat ctttatcgat ccctttttcc ttcagtactt cgcccatggc gttttgtaac
     9421 cagtaaccga tagatccttc tgtcattgca acacatgtat ctaaaggcat tgccggtgtt
     9481 ttctcagaat cagcagcttg ctgctgaatc aataggttac cgacttgcgg accatttccg
     9541 tgggagatga tcaattcatc tccttgttcg ataaatttca ctaaatactt tgctgtttcc
     9601 attaacgctt cttgttgggc ttttgcactt gcatccgtag acaagatggc attgccccct
     9661 aaagcgacaa ctacttttcg tttggccatt tcattcgtcc tttcttcagt tctaccgaat
     9721 tttctagata acaaaacgag ccagcgtttt gtgattttcc ctcaatgctg actcgttttt
     9781 tcgtgagatg aatgtggtta tgcttaaacg cgagggataa acaagttacc taatgttgct
     9841 gccatgatcg ctttgattga gtgcatacga ttttctgctt ggtcaaattg acgagcatat
     9901 ttgctgcgga agacttcatc tgttacttcc atttcagtga tgccaaagcg ttctttcatt
     9961 tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa
    10021 cgatctgtgt tgtgtgtttt ttctaccatt tccatattga tttggtaagg tttcaacaat
    10081 ttgatacgtt cttcgaattt atcttcttca cccattgata cccatacatc agaataaagg
    10141 acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat
    10201 ttttcagcaa agccttctgc cattttgacg atttcttctt caggttgtaa ttcttttggt
    10261 gcaacgatac gcatattcgc tcctaaaatt gcacctgtga ctaataaaga gttagccata
    10321 ttgtttcgac catctccgca gtaagcaaca gtgatacctt ctactgttcc aaagttttct
    10381 tggatagtta aaaagtcagc gatcatttgt gttggatgcc attcatcagt caagccattc
    10441 caaactggaa caccagagaa ttctgctagt tcctctacca ttttttgact gaatccacgg
    10501 aattcgattc cgtcaaacat acgtcctagt actttagctg tatcttctgt ggattctttt
    10561 ttacctaatt ggatatcatt tgcacctaga tattcaggat gtgcacctag atcgatcgct
    10621 gcagtagtaa atgcagaacg agtacgtgta gatgtttttt caaataaaag cgcaatattt
    10681 tttccttcta gatagtgatg tgggatacca cgttttttca agtctttcaa gtgttctgaa
    10741 aaatcgatca aatattgtaa ttcttctttt gtaaagtctt tttctgctaa taagcttctt
    10801 ccttggaata ctgattcttt catattaaac attctccttt agattgtttt tatttaagat
    10861 taaagaggaa atcccgacgg cagctgacag taagactgcc gtcaggaaac aaattacttc
    10921 aagtcttcac ggacaagtgg ttggctcatg cagcgtggac caccacgacc tcgtgacaat
    10981 tcacttgaat tgatttctaa cactttgatg ccatagctac gaagcaattc gttcgataca
    11041 taatttcggt tataagtgac tacaacacct ggagcgattg ccaatgtgtt tgatccatca
    11101 ttccattgtt ctcgaggtgc aacgatctca tctccattac cagttgggat cagtacaaga
    11161 tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg
    11221 atatcatcac catctggttc gattgtaaag acatccatct taccgtcttt actttgaatt
    11281 gctggatgga tagtgaattt atcgtgatct accattgtaa atacggtatc caaatgcatc
    11341 attgcacggt tgttcggaat tttgattgcc aacacttttt caaatcctga attttttgcg
    11401 aataggtggc gtgctaattt ttctaaagct ttcgcattag ttcgttgaga aatcccaaca
    11461 gctactactt tatcgcttaa gatcaattcg tctccgcctt cgatatgatc tggattttca
    11521 cgatccaacc atacttcgat tcctttattt gcaaatcttg gatgatgttt caaaatatat
    11581 tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca tcttattgac tgtcatccca
    11641 tttcccattg atgcggaagg gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa
    11701 aatggatagt catcgtctgc tgacagatca tatagactgc tagaacgtac ttcgatatct
    11761 tttgtacgaa ccccagccat gattttatct accatttctt gcgtttccat tgatagaagg
    11821 aactcatgaa gaccctctct tacagcattc gatgcaatat gagattcatc caacatttta
    11881 ttcaagaatt gttctttgac attgcctgcg tcaatcgctt ctgccgctaa tttttccaag
    11941 taaagagtct cgacgccttc attttgcaat gtcttcgcaa agttgtcatg ttcttcttga
    12001 gcaatcggaa gatatggaat atcatcgaac aacagacgat ccataatatc tggtgtcaaa
    12061 ttttccactt cttgtccagg ccgtttcagt aaaactgtct ttaatttccc tatttcagag
    12121 aaaacgtgaa taggtttatc catgttgtgt tgcctccttc tttttgatta catgtatagg
    12181 atatcctcct tttgtaatca ctttcataat tttgtctcat tcaaaatgag acattcctca
    12241 cattaatatt cgataattaa ataattaaac gtttttctat ttttaagcgt aatttattca
    12301 ttttttattc tgtatacact ttcaaaaaaa tatttattca ttgaaaaaat ttttttgaat
    12361 aaattaaaaa aataaattat attatcgttg ttagcgttta ctatatgtat tattgttgtt
    12421 ttttatacac aaatcattcg cttcaaaaca aattattcaa taccattgta tattttttac
    12481 ataataacaa attatatttg taaagtatta aaacttgttt ctaatgttgg tatgttaaaa
    12541 ctttacaaat tctttaaaaa aagtgacaat aaatatagtg aacaataagg agaggacttt
    12601 ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt
    12661 gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat
    12721 aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc
    12781 aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat
    12841 gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat
    12901 acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa
    12961 ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt
    13021 gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa
    13081 caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc
    13141 attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact
    13201 gctggacaag taatcaaaca actacgtgat tccgggctgt tagattatga caaaaagcgt
    13261 tttactttct gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa
    13321 gcagtgaccc tgataaat
//
LOCUS       DAPBAB010000063        13146 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPBAB010000063 DAPBAB010000000
VERSION     DAPBAB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.066"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(77..149)
                     /locus_tag="RD444_002238"
     tRNA            complement(77..149)
                     /locus_tag="RD444_002238"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="RD444_002239"
     CDS             530..1099
                     /locus_tag="RD444_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDT7549595.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="RD444_002240"
     CDS             1086..1946
                     /locus_tag="RD444_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7549596.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="RD444_002241"
     CDS             2136..2840
                     /locus_tag="RD444_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7549597.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="RD444_002242"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="RD444_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDT7549598.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="RD444_002243"
     CDS             4677..5993
                     /locus_tag="RD444_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549599.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="RD444_002244"
     CDS             5994..6863
                     /locus_tag="RD444_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDT7549600.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="RD444_002245"
     CDS             6918..7727
                     /locus_tag="RD444_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7549601.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="RD444_002246"
     CDS             complement(7830..8459)
                     /locus_tag="RD444_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDT7549602.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="RD444_002247"
     CDS             8629..9921
                     /locus_tag="RD444_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549603.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="RD444_002248"
     CDS             complement(9997..10659)
                     /locus_tag="RD444_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7549604.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="RD444_002249"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="RD444_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDT7549605.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="RD444_002250"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="RD444_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDT7549606.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DAPBAB010000064        12859 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPBAB010000064 DAPBAB010000000
VERSION     DAPBAB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.067"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(115..450)
                     /locus_tag="RD444_002251"
     CDS             complement(115..450)
                     /locus_tag="RD444_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7549607.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            736..2616
                     /locus_tag="RD444_002252"
     CDS             736..2616
                     /locus_tag="RD444_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDT7549608.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2695..2922
                     /locus_tag="RD444_002253"
     CDS             2695..2922
                     /locus_tag="RD444_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7549609.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2927..3469
                     /locus_tag="RD444_002254"
     CDS             2927..3469
                     /locus_tag="RD444_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDT7549610.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3720..4397
                     /locus_tag="RD444_002255"
     CDS             3720..4397
                     /locus_tag="RD444_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDT7549611.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4718..5959
                     /gene="sstT"
                     /locus_tag="RD444_002256"
     CDS             4718..5959
                     /gene="sstT"
                     /locus_tag="RD444_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDT7549612.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6106..6741
                     /locus_tag="RD444_002257"
     CDS             6106..6741
                     /locus_tag="RD444_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDT7549613.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6932..7675
                     /gene="truA"
                     /locus_tag="RD444_002258"
     CDS             6932..7675
                     /gene="truA"
                     /locus_tag="RD444_002258"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDT7549614.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7909..8742
                     /gene="msrA"
                     /locus_tag="RD444_002259"
     CDS             7909..8742
                     /gene="msrA"
                     /locus_tag="RD444_002259"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7549615.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8856..9816
                     /locus_tag="RD444_002260"
                     /pseudo
     CDS             8856..9816
                     /locus_tag="RD444_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            10105..10803
                     /locus_tag="RD444_002261"
     CDS             10105..10803
                     /locus_tag="RD444_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDT7549616.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10953..12269
                     /locus_tag="RD444_002262"
     CDS             10953..12269
                     /locus_tag="RD444_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549617.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVLNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            12285..12743
                     /locus_tag="RD444_002263"
                     /pseudo
     CDS             12285..12743
                     /locus_tag="RD444_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         4183 a         2085 c         2702 g         3889 t
ORIGIN      
        1 gtaaccgata cattagtttc aatgtttact gagtaactaa ttattttgca ggaggacaat
       61 ttatttacac aaaattattg acgctcccaa aatactcgtt tacataaaaa aagattattt
      121 agatgcgata atatcataga taaagaaaca ttcatcataa gaaacagtga tcccaaattc
      181 tgtttcaata tttttgaagg ccttttttac atgaaaaatt gtttcatgag aaggtcttgt
      241 gtcatcttct gatataattt taagttcatg accgtttatc aatgtacgct caattagaca
      301 cgctgtatga atcaaaagtt ttaaaagaat ggaatgagtg aattttttgt ggaaagtaca
      361 ttccaattct tggataaaat ccattaagct tttttggatc ttattcccag ctaataagta
      421 gagatatttg tccactgctt caactcccat atcaaaaaac gatatttctt gtgaatgagt
      481 ggctgctgcc cttttttgat tgttgttctt cagtcgtaag atgacaaagc atcttatctt
      541 tgaataaaaa aatagaacta cctaaggaag cgtttcggcg cttccttttt ctatatgatc
      601 gtttaatgac tgtaggaatt cagtatacca aaagaaaacc gcctttttga ataaacttga
      661 tagtggtcaa ttttttatga tggataagta tctatactta gtctcgtaag aatgaaaagg
      721 aaaagaggga gaaagatgaa attacaaaag tggatccaag agcatcgaaa tcatatcacc
      781 gccttatcag ctgtgttgat tatttttggt tttatcggga aatttactgg atttgtcttg
      841 ttctataaca ttgctctagc catagcttct attattgcgg tcatacctat tataatccat
      901 gcgtatcagg cagtaaaagt caaagttatc tcaattgatt tactcgttac tattgctgtg
      961 cttggtgcct ttatgatcgg agaatacaag gagtcagcta tcgttacatt cttgtttttg
     1021 tttggtagtt atctggaaca aaaaacattg accaaaacgc gggaatcaat caaggctttg
     1081 accgagatgg ccccatcgac tgccagcgtt gtccaagaag atggaacgac cgaagaagtg
     1141 gaaatcgatg acgtggaaga aggcgatatc gtcttagtaa aaactggtgg agctattcct
     1201 gtggacggac gagtcattga cgggaaaggg tatgcggatg aagccgcagt gacgggtgaa
     1261 tcacgacaag tcgtcaaaat cagtggagat aacgtatttt ctggaacgat tttagcagat
     1321 ggctacgtga cgatcgaagc aactaaggtc ggagatgata cgacctttgc caaaattata
     1381 gaattagtcg aagaagcaca agataccaaa tcacatgcgg aaaaattcat tgatcaattt
     1441 tctcagtatt atactccaac cgttttactt attgcgttgc ttgttggtct gatcagtcgt
     1501 gattttcgtt tagccatcac tgttttggtt cttggttgtc ctggcgcttt agtcattggt
     1561 gcgcctgttt cgaatgtagc aggaatcggt aatggagcga aacggggcat cttagtcaaa
     1621 ggcggtgaag caatggatac tttttccaaa atcgatactt ttgtctttga taaaaccggt
     1681 acattgacca aaggaactac tgctgtatcc actatcaaga actatacaga ggatgaaggg
     1741 gacagcttag ctttagtggc tcgattagaa acgttatccg atcatcctct ggggcgcgcc
     1801 gtgatccatt atgctcagga gaaagacatt gattttcaac aattagcagt caaagataat
     1861 cagacgatca agggacaagg aatcacagct gagattgatg gtcatacagt ttgtgcgggc
     1921 aacaccaaat tgattgccgc tcacaaactt tctctcacac caaagcaagt acaagattta
     1981 catcaattac aacaaagagg cagctcagtc attattgtag caatcgacaa aaaaatcact
     2041 caaatcattg gtgtgtcaga tgtgattcga ccagaagtcg ctgaacaatt agctatgttg
     2101 aaacaagcag gagcaaaaca tttagtgatg ctgacaggtg ataatcagat gactgctgat
     2161 tatgtagctg acatgttagg cattgatgaa gtccatgctg aattattgcc agatgaaaag
     2221 gttcaatttg tgaaaaaata ccaagaacaa ggattgcacg ttgctttcat tggagatgga
     2281 atcaatgatt ctccctcttt ggctgcggct gatatcggga ttgctatggg atcaggaacg
     2341 gatgtcgcta tcgaaacgag tgacgttgtc ttgatgcaat ctagttttgg atcgttggtt
     2401 catgcttacc gtttggctaa gaaaactgtt ttgaataccc gtgaaaatgt agtgattgcc
     2461 attggtgtcg tcttattttt actcatcggc ttatttgccg ggtttattta catggctagt
     2521 gggatgttcg ttcacgaagc tagtatcttg atcgttatct ttaacgcgat gcgtttattg
     2581 tattttggac acgaaagcaa agaagaaaaa tcgtaaaact tgataagaat caattttttt
     2641 atggaagctg taatctagaa taagactatc aagaaaaagg aaggaagaac tacgatgaaa
     2701 aaaatgatta tgcaattaga tacactagct tgtccaagct gtatgcaaaa aatagaacat
     2761 gcggtttctc aacagcctgg ggtaagcaat acgaaagttt tgtttaatgc gagcaaaatc
     2821 aaagcaaact ttgatccaga tgaagtccac cctgaacaat tagctgcagt tgtggaaaaa
     2881 ttaggttatg aagtccaatc aacaaaagta aaagaggcgt aaatcagtga atatagtgga
     2941 agaaaaattt gctcaagaaa caaaacaagc agatatcgat catcataaac caactgctgg
     3001 cgccatggtg gggcatgtat tggcaaattt taaattagaa gaaatgaaat tgatccaagc
     3061 acaattgtat gttttgggta catcttcgca agaattaagt accctttttt cagaatatgc
     3121 tgatcaagaa gcgagatggt tcaataaaat ctctcatgag ttagtgattg aaaatgaaat
     3181 cgttccgtct actttagaag aagtcatgcg ttatggaaaa atttacgaac atggtcaaaa
     3241 caaatattta ccagcctcaa caatgttgga aaactttgca aaagattttg atttccaaaa
     3301 tgtctttatc acacgggcaa tcaaattagc tgaaaaagaa gaaaaatatg gcttgaaaca
     3361 attgcttgtt gaacttttaa gtttcaataa agaaatcatc taccaagtac aaagactcca
     3421 aggaaaaact gtccgacaag atttggatga ggaagacgat gatgactaaa agtctatgtt
     3481 tcttcaaata caaatataaa gcgtgaggta aattggaaaa tcgcgaagat taaaataaaa
     3541 gaagcacccg ctgatgtatt acagacaaca tcagcgggtg cttcttagtt tttaaaaggt
     3601 ttaaaagatg gatgagaaaa tacaaaaaat caataaaatc acattgctta tttgatagaa
     3661 taaagaagtg ggagtaatcg aaaggagttt tttaatttaa aaaaaggatt ttagtaacaa
     3721 ttggtctagc ttttttgtta gctggttgta ccaacgggaa tgaatcgaaa aaagaatcaa
     3781 caaaagaaac agcgaaagca acaattgcaa ctactacaac aactagcaca gagagcaagc
     3841 caactgcagc aagtacggaa acagcagata cctctacttc gtcagagtct tccaaaacga
     3901 ctgaaacaac tcaaacatct acttcaagtt cttctgtaga ggcttcatct tcagaaagca
     3961 gcgaacaagt cgaacaaaca ctgtggaatg cagacaaatc cgcacaatta gaggcattta
     4021 tggctacatg gggccaaaca atgggacaac aatacaaaag ctatacgaat caaatgagtg
     4081 tggacttata tggtcttaaa gtccctcaag ttattttgaa cggggagtgg aagatggcta
     4141 ttggtggagt gcctgtttct gcagaatggt cagaaagtgg gacaggtcaa gcagactatc
     4201 aaatcacagc cgtctattcc gatgcagaga cagaaccata tctgaaaaag cacgtctatc
     4261 tattcgggtt tcagcaaaat cagccaaaag tattggtgac acaacaaaat caggggaatc
     4321 ccgataatta tctttacttt aacgagacag cgaataatga attgaaaaat ggctttaatc
     4381 aaatcgttta tgggtaagta aaccagtgag agtgagagaa agaagagact cagtggaaaa
     4441 tattaattga ttgctatgtc taagaattaa gatgaaggat gaaatttcta gtttcgtcct
     4501 tcatcttagt tacgttttac ttggataact ataataataa cgataaatag ttttctctcg
     4561 acttctttag agattgaata aaccctccaa tactaaaatt gaatgttgca agagcatgaa
     4621 ttgccaattg tattattaga atttttagaa aattgcacct ttagtttctt tagataaaat
     4681 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     4741 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     4801 ccccacgtgg acatttattt caatattggg gacgttgttt gtcagttgct tgaaggcaat
     4861 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     4921 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     4981 tgtagcagtc attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     5041 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     5101 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gttttattct gggctgtatt
     5161 gttgggacta gcttttcgag cacgttcaga accgacaaaa gagttggtgg atcagatatc
     5221 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     5281 attggtttat caatcaattg ctacgactgg aatcgctgga ttggcagagt atctgcagct
     5341 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     5401 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     5461 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     5521 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     5581 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     5641 agggattgaa gcaccatttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     5701 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     5761 gtttggtatc tctaatgatg tcgcgatgca aatcgttggt attggattta ttatcggtgt
     5821 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     5881 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     5941 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     6001 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgttt
     6061 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     6121 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     6181 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     6241 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     6301 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     6361 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     6421 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     6481 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     6541 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     6601 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     6661 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     6721 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     6781 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     6841 ctgtcttaga gaagcaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     6901 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     6961 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     7021 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     7081 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     7141 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     7201 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     7261 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     7321 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     7381 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     7441 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     7501 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     7561 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     7621 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgct cataaaaaag
     7681 aatcatcttt actcgacttc tgagaattgg agaacatccc ttttttcaga ggtcgttttt
     7741 tagttaaacc aaaccataga gtatgctttt taagataaag atcatcttat atcatctgaa
     7801 gctgcttatc ttctttgaat aaatacgatt caatcgatta tttcctcact ttatgataaa
     7861 ttacagctaa agtcactagc ttgtcttctt cggaaaggag gagaatctat ggatgaggaa
     7921 caaatattca ttcaaacact ttataatctg attttgaatc cgaatacacg agattgggaa
     7981 cgaaaagtat tgattcagac aaagaatgac acaagagaaa atatatccgt taaagaacag
     8041 ttatcaaaac tagaagtaac actacgtcca ttagctatcc gaatgaatct gacgcctgat
     8101 gtaatggact tttatttatt gattacggaa ggatttgata aggagcaaaa atatgacttt
     8161 tccaagcatg ccatgcaaga tgctgactat caagaacgtg cagtatttgc agggggatgt
     8221 ttctggtgta tggtcgagcc gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga
     8281 tacacgggcg gacatgttga aaaaccgaat tatgatcaag taagtggtgg ttacacaggt
     8341 catgtggaag ctgtggagat catttatgac acaagagaaa taagttatag cgagttgctg
     8401 acaatctatt ggcaaattac tgatcccaca gatacatttg gacaatttca agatagaggg
     8461 aagcaatatc gtccagtgat tttttatcaa gacgaacgac aaaaagaatt agcggaacaa
     8521 tcaaaacaga aactagacag ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa
     8581 ccagccggta cgttttggcc agcagaaaac tatcatcagc aattttacaa aaaacaaccg
     8641 aaacgctata aaaaaatcca gcaagcacgt aatcaatttc tgatctatca acgagtaaaa
     8701 aacaaatggc aaaaaaatat acgaaaaaat cattttgatt aactcctttt ctttaaaatg
     8761 tagtaaacta aaaatagaaa acaagaaagc gttttaaaaa gatttgatga ggaggagaaa
     8821 aattgtatat tgcagatagt gcacgttatg aaaaaatgga atatcgtaaa tccggtcatt
     8881 caggtttaaa attaccgatt ttatctctcc tctcggatta tggcaaaatt ttggtgatta
     8941 tgatcctatc cataatcaaa gagagatttt aagaggcgca ttcgacatgg gaattaccca
     9001 tttcgacctt gcgaataatt atggaggccc tgctggagct gcagagaaaa actttgggcg
     9061 tatttttaga gaagattttc aagcctaccg tgatgaactg attatttcta gcaaagcagg
     9121 ttatcatatg tgggaagggc catatgggga atggggctct cgtaaatcca tcatatcaag
     9181 ttgtgaccaa agtttccaaa gaatggggct agattatgtt gacatcttct atcatcatcg
     9241 cccagaccct gatacacctt tggaagaaac agcagaagcg ttgatgcaac ttgtaagaca
     9301 aggaaaagca ttatatattg gtatatccaa ctataatgga gaagatacaa aaaaatgact
     9361 gaaattttaa aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt
     9421 tctcgtgcac tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca
     9481 atcacgttca gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct
     9541 gaagattcta gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca
     9601 actctggaaa aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg
     9661 caaatagccc ttgcatggaa tttacgacaa aaatctgtta caagtgtact gatcggagca
     9721 agtcggttaa gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca
     9781 gaagaagaat tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat
     9841 ccatatatta aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg
     9901 aacatcatat tttttgtcga tcaaaataaa aatgatcgaa ggctaggagt gaattttatc
     9961 ctagtctttt ttatggtaaa tatcttcttc gtttgtatgt ctatgaacgg attaatcgat
    10021 tttttataca aaaaatcatc agatgattca aacgtgttat aataattacg aaaaaataca
    10081 tcagatgaaa aggaggtatg tggtttggaa gaaaataaaa ccctagtaga agtaactact
    10141 gacaagatcc ttagatattt aaaggaaatg cagttgcaag taggagataa actgccgaat
    10201 gaatatgaat tgtccgatta tttagaggtg gggcgaagca cggtgcgaga agctgtgcgg
    10261 gctctagctt ccaggaatat ccttgaagtt cggcaaggag cggggacatt tgtcagtgat
    10321 aagcgaggga ttgccaatga cccactcggt ttttctttga ttgaagatca ggatcggatg
    10381 atacgagact tatttgaact gagatattta ttggagccta agatggcagg tttggcagcg
    10441 gaatatgcaa cgaagaatca aatcgtccaa atgaaaaaat taacgcaaaa gattgaaact
    10501 tcttttcgag aaggcacaca agaacatgtt caattagaca tcgattttca tacgatgatt
    10561 gcagaagcta gcggaaacat agccttgctg catattatcc caatcattaa tgaatcgatt
    10621 gctttgttta ataaaaatta tgatatccag catctgaaaa tcgagacgat cgatattcat
    10681 acagagatcg ttcaggccat cgaaaggcgg aacgtacttg ctgcgtttga cgcaatgact
    10741 gtccatatgg caataaatcg gagagaatta cagaaaataa taggagaaaa agaaactaat
    10801 tgacagttca tctgatgtat tttatgataa atacatcaga tgattttttg ttttatattt
    10861 ggtaaaaaag aggagtaaat agtttctgtt caccaaaatg taagcgctga ctaatagaca
    10921 aaaagagatg acgtcaagga gaagaagcag gaatgagggg atctggaatg agttttatgg
    10981 ataaattagc caaagtttta gggaacgtgg caacgagaat caataattta aggtatatca
    11041 tggtgataaa aaacgcattt gctgcattga ttccggtaat tattacgggt gcttttggca
    11101 cattgttttc agcaatggtc tttgactcgg aaaatggctt agcacagata agttacttgc
    11161 gttttttaga agaattaaag cctatcgcat ctgccatcag ctatgtcaca ctaagtttct
    11221 tgaccatcta tgctgttttt ctaataggga tcgaattagc caagctgaat aaagtggatg
    11281 gtgtattccc tggtatcatc gcagtgatgt cttatttatc tgttaatcca actgtttatg
    11341 aattagtgaa tgaaaatcag actgttatag tagaaaacgt attggctaaa caatatacag
    11401 acacgaaagg gttatttttg ggaatgttgg tctccatcct ttctattgaa ctttattgct
    11461 ggttgaggaa acagaagaaa ctacagctga gaatgcctga taccgtgcca acgaatgttt
    11521 ctgctagttt ttctgcttta ttcccgacga ttctgactgt atcgggtatc gcggctgcgg
    11581 gatttatcat caaagaactt acaggaatgt acctttatga tatcatttac aatgtcgtac
    11641 aacgcccatt agaaggtgtc gttcaagggt taccgggtat tttgctattg atgtttattg
    11701 cgcaagtctt ttgggtgatt ggtatccatg gaaatcaaat ggtgaagccg atacgggaac
    11761 cgttgctttt agcggcaatt gctgtaaata cggaagcttt tgaagctgga aaagaagtac
    11821 tgaacattat tactatgcca ttttgggata tgtatatgag tatgggtggg tctggagtca
    11881 cgatcggttt actgattgca gtattgttag ttggaaaacg ggaggatatg aaacaaatca
    11941 ccaagttatc ccttgctcct gggatcttca acattaatga accagttatt tttggtatgc
    12001 cgatcatgct gaatccaatt ttagctattc cgtttatctt aacgcctctt atcacaggaa
    12061 cgattggtta ttttgcaacc gctttagggt ttgcggcgaa agcagttgta atggtccctt
    12121 ggccaatgcc gccgatcgtc aatgcttatc tggcaacagc aggaaatatc ggtgcagtta
    12181 ttacgcaact tgtttgtatt attgtttcta ttttgatcta tctgccattt gtaaaagtag
    12241 caaatcgtgt ggaaaatgat gggatctaaa aacaggagga aatcatgaaa attactattc
    12301 caaaaaattt tattcttggt gctgcatctt ctgcttggca aacggaaggt tggaaaggaa
    12361 aaaaagaagg acaggattcg tttatagata gctggtataa aaatgaacat tttgtttggc
    12421 ataatgggta tggcccagcc gtagcaacta attttatgga acaataccag caggatgtgg
    12481 atctgatgaa atccatccat ctgacacatt atcggacttc tatcaattgg gctcgttttt
    12541 ttactgatta tgagaactgt atagtagatg aagaatatgc tcagcatata agtgaaatga
    12601 tcgactcttt gatcgaagca gacgtagaac cgatgatttg tttggaacat tatgaagtcc
    12661 cagcttttct tttggaaaaa tatgatggct ggtcttcaag aaaagtagtt gatttttatg
    12721 ctaagtatgc tcaaattgcc tttgtggtgt tagtatcaaa tcttagacag cttttcgtag
    12781 agacaagaat aaattaaact tatctacgag aggatgaatt tttatgaccc gatatgaaga
    12841 aaattttaaa caaatgatt
//
LOCUS       DAPBAB010000065        11845 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPBAB010000065 DAPBAB010000000
VERSION     DAPBAB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.069"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(37..456)
                     /locus_tag="RD444_002264"
     CDS             complement(37..456)
                     /locus_tag="RD444_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HDT7549618.1"
                     /translation="MKKIIVATHYRLAEGFKSTLGYIAPNMVEIIAINAYLENTSVEN
                     QVQAALDQFEAEEQILVFTDLLGGSVNQTFAKFLDRYNIQLIAGVNLPILMTLALAIG
                     SNDLSETTIRQAIEESRQQIVYVNDLLQVSAMDDMDE"
     gene            complement(477..1307)
                     /locus_tag="RD444_002265"
     CDS             complement(477..1307)
                     /locus_tag="RD444_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7549619.1"
                     /translation="MNNERTKGLTQELNKKDRRNAAWRWSFIGSNNINYGTLQGTGYA
                     WSMAKTLRKIYPNDDDYVEAMRVEYEYFNITPYMGPLVAGADVALQEQQGTESLGAVR
                     SIKTSLMGPLSGIGDSLLWVLYPTIMGSISGYMALEGNPLGAIIWIILNLGLVWMRLK
                     LFDLGYESGIRLITDMADKLNAITESASVMGLTVVGSLIATVVKVYTPIEFTFGEVTM
                     GLQAEVFDRILPALLPVLLTALVYFLMEKKKWGFLKVIFLLLAFSLLGAYFGFLGVAP
                     "
     gene            complement(1300..2088)
                     /locus_tag="RD444_002266"
     CDS             complement(1300..2088)
                     /locus_tag="RD444_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549620.1"
                     /translation="MPEFLQNILIILIPMLTLIDNRGITIINHWPVTVGLLAGLVMGD
                     LQSAMVIAGTFQLMSLGVAALGGSSVPDYGLATVVAIYLNARTGAEIGTSIAVGLPVG
                     ILAINLDIFSRTINSFVSEKSKQYLEERKFSKMQWINFISVFFVAMQAFIPMVILVAF
                     GQVAIETATQWIPEWITNGLNVAGGMLPVVGVAMLMRYMPTKRYIWALLAGFIMAAYM
                     ELPILAVSIVGLALAVFIYNNLTSQKVAVTTENAAYDEGDDFDE"
     gene            complement(2113..2586)
                     /locus_tag="RD444_002267"
     CDS             complement(2113..2586)
                     /locus_tag="RD444_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549621.1"
                     /translation="MGLVLARIDERLVHGIVVTQWASAVKAKRIMVVDDQVAQDEPRK
                     AAMRLSKPAGTGMSLIDTETGITNFKAGKYDSHNVLLVVNNVKTLLDLSEQGIAIPKV
                     NIGIMLDRNDRKKLAKNFAASPEELQQLQQLADKGIPVSYQFAPSDKEEPLDKFI"
     gene            complement(2603..4660)
                     /locus_tag="RD444_002268"
     CDS             complement(2603..4660)
                     /locus_tag="RD444_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 31"
                     /protein_id="HDT7549622.1"
                     /translation="MLFKSKEVIVSEQSFVVKDRNQCLKVEGWGRDTVRVTCYPNHKG
                     FDPKQGIEELEKNVLTTATVTETSDQLILTNNRLTATYDGEKLVFYNNGTIILEEYSR
                     KQSQVRRTVGIDTHIPIPDEPTSSLNISPREFTYSGENTYQATLRFEGDPNEKLYGMG
                     GYQEENFNKNGGTYELMQRNSQTSIPFYLSDKQYGFIWNNAAIGEVTFGYNQKIWKSN
                     NTNLIDYLVTVGENPKQIIENYTQMTGRTPRLEPELLGLWQSKLRYQTLDELRTVYEG
                     YNQRGISLSVLVVDYYHWTSDGDFAFDSRYWEGIEDFAEEIKKTGTQLMVSLWPTVTE
                     ESRYYQSYRNNQLLIRSVTNKDSLFAGKALLDFFHPESKTFLRQVLDENYRSKGISLF
                     WADQAEPEMDVYQHKEYLIYEGSLEKYANKYPLHYLQAIQKNPERSNTEPTLIRSAWF
                     NAQKYGALAWSGDIESSFDSLRRQVQVGLSMGISGITWWTSDIGGFHSGNSQSPVFKE
                     LMIRWFQFAVFSPILRMHGDRQPHTQKIGNSGGGVRTSGGPNEIWSFGEEVEGILTRF
                     IHIRTRLKDYIRDVYNESATKGLPIMRSLFIEFPDDPKSWEETTHYLFGSDLLIAPVV
                     TEGATHIPVYLPKGCNWIQVFTKKVYPGGQIYKIAVTLNTIPVFCKEGAPLEHHLADI
                     FGN"
     gene            complement(4710..6767)
                     /locus_tag="RD444_002269"
     CDS             complement(4710..6767)
                     /locus_tag="RD444_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDT7549623.1"
                     /translation="MITIEANRIVKKYDDELLIIEPWGQHSLRVRSFLDQHFVERENA
                     LTELHGYDEKLVETYYKNNQATIVNGKIKAVLDHRDRLTFYNEKDEVLLKEFIRLRAV
                     KHDDGSEDVGTVEFTKDFNSTLKLKSREYRANNDGSNFRTKVRFEPHAQEKIYGMGQY
                     QQNYLDLKNTMLELAQRNSQMSVPFYVSSRNYGFLWNHPGIGKVTFAKNMTEWEMYGT
                     DYIDYWITAGDSPKEITEQYTSVTGRVPEMPKKLLGLWQSKLRYRTPEEVLEVVEGYK
                     QRGIQLSTIAIDYFHWPKQGEYRFDPDFWPEPEKLVKTLKEDYNVEPLISIWPTVQSD
                     AYNYEQYLQGGYLIKVNRGVRLTMQIQGSTVFMDTTNPEARDYVWNLIKKNYKDIGID
                     YYWVDVAEPGYAVYDFENYRYHSGTAVESGNLYPNGYLKMVYDGNKDSNESVVTLVRG
                     AWAGAQKYGALAWSGDIDSSFEAFHNQVNSGLNMGMAGIPWWTTDIGGFHGGNPEDPE
                     FRELMVRWFQYSTFSPILRMHGDRLPHSKPLSNSGGGSMPTGAPNEIWSYGSEVENIM
                     TDYVEIRERMKDYLAESMTEAHELGYPIMRTLFYEFPEDKKSWEIDNTYMLGSDILVA
                     PIMNYKERGREVYLPSGEKWRHLFTGKEYAGGATYYVAAPLEQIPVFIKESASELFES
                     LGK"
     gene            complement(6790..8283)
                     /gene="xylB"
                     /locus_tag="RD444_002270"
     CDS             complement(6790..8283)
                     /gene="xylB"
                     /locus_tag="RD444_002270"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDT7549624.1"
                     /translation="MGYVLGLDLGTGALKGVLVDETGVIRHIEACDYPLHSPKGGYNE
                     QNPADWLTACGALFEKFSCNVSDFQAELAGISFSGQMHSLVVTDEAGTVLRPAILWND
                     VRNSAQCKRIMDEFGNEIIAITKNRALEGFTLPKILWIQEHEPAIWEKVRKIFLPKDY
                     LRYYLTGHSHMDYSDAAGTLLMDTTKKEWSKPILEKYNIPEVILPPLVESSACVGTLK
                     PEIKATFGFEKEVRIFTGGADNAVSSLGSGLTDSHTALASIGTSGVFLALEEEDHTRY
                     GGKVHLFNHVLPDTYYAMGVTLAAGHSVSWFKNLLAPEQSYDEFLRNIPNVPIGSNGL
                     LFTPYISGERTPHVDSQIRGSFIGLSNHHTRDDLIRAVLEGVTFSLKDSQQLMTSLKG
                     EEFKRIISVGGGAKNKTWLQMQADIFNTEVVSLDTEEGPGLGAAILAAVGLGWFDSFE
                     SCTNQVIRYGTTVQPIPENVEKYNTVYDLYTKVYGATKDLSHALSIL"
     gene            complement(8302..9609)
                     /gene="xylA"
                     /locus_tag="RD444_002271"
     CDS             complement(8302..9609)
                     /gene="xylA"
                     /locus_tag="RD444_002271"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDT7549625.1"
                     /translation="MNYFEAVPKIQYEGKQTKNKFAFRYYNPEEEILGKPMKEHLRFA
                     VAYWHTITADGSDPFGAGTYQRNWEGNNPMETAKNRVEAFFEILEKLGAEYFCFHDVD
                     IAPQGDSLEEFFQNIDTITDLIKEKMDQTGIKLLWNTANMFTHPRFVNGAATTNNADV
                     YAIAAAQVKKGLDISKKLNGQNYVFWGGREGYESLLNTNMDFELDNLARFYKMAIAYT
                     KKIGHQPQFLIEPKPKEPSKHQYDFDAATSMAFIMKYGLQDHFKLNLEANHATLAGHT
                     FEHEINVARSYNALGSLDANQGDLLLGWDTDEFPTDIYSTTLAMYEVLENGGIAPGGI
                     NFDAKVRRTSFEMEDLILAHIVGMDTYARGLRAAAKMKEERFFEEIMEEKYASFTQGI
                     GKKIIDDQETFESLSEYALSLSDVTNQSSHIEYIKSLINDYLV"
     gene            10041..11012
                     /locus_tag="RD444_002272"
     CDS             10041..11012
                     /locus_tag="RD444_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7549626.1"
                     /translation="MASVSGGRKPIYLSFNNECALVIAIEIGKDYIHSSLSYINGELV
                     NKLSYHSLLVTKDTLVMLLTKIISDLTSKIPQTPYGILGIGMAIHGIVKDDTVLFTPY
                     SDIDSIPLKDLLAKEIPYPIVLINEANAGALGEYTFKTPSENLVNINIQDGIGAGIVE
                     NGKLFIGANGTAGEIGHTTLYPDGILCPCGNHGCLEHYASTRILLKKLLDSDHKPHPD
                     ISVIVKKWHQGDAHTQKILKHNAKLLSIGINNIVLMYDPEVVIINSEIYRGIPELIDE
                     IKKHLTGKNTKNLAIKNTSLKGNSTLYGCVSIVAQSFLKINNLKFTV"
     gene            complement(11103..11414)
                     /locus_tag="RD444_002273"
     CDS             complement(11103..11414)
                     /locus_tag="RD444_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549627.1"
                     /translation="MRNKPITVTLFANAYDNELKTNPHSKLKEGTYSVDNDRISFLTK
                     KGEGLVLNLNIQHIDSIATFNQSVIQIKDHNMTEVKNKIYAFSKKSNDKSYWETLAEI
                     I"
BASE COUNT         3237 a         2622 c         2264 g         3722 t
ORIGIN      
        1 gcaaaaagat tgaacgaatt caatcttttt gcttttttat tcatccatat catccatagc
       61 tgatacttgc aacaaatcgt taacataaac aatttgttgt cgggactctt cgatggcttg
      121 gcgaatggtt gtttcactca aatcgttgct accgattgct aaggctaaag tcattaagat
      181 tggcaaatta acgcccgcaa tcaattggat attgtaacgg tctaaaaatt tggcaaaagt
      241 ctggttaacg gaacctccca acaaatccgt gaaaactaat atttgttctt cggcttcgaa
      301 ttggtctaat gccgcttgga cttggttttc aacagaagtg ttttctaggt acgcattaat
      361 agcaatgatt tccaccatat ttggtgcaat atagcctaac gtactcttga acccttccgc
      421 taagcggtag tgtgtagcta caataatttt tttcacctat ttccctcctc gtatgattaa
      481 ggtgcgactc ctaagaaacc gaagtaggcc ccaagtaaag agaacgcgag cagcaagaag
      541 ataaccttta aaaagcccca ttttttcttt tccatcaaga agtaaaccaa tgcggtcaac
      601 aagaccggta acaatgctgg taagatacgg tcaaagactt ccgcctgcaa gcccatggtt
      661 acttcaccga aagtaaattc tattggcgtg taaactttaa caacagtcgc aattaagctt
      721 cccactaccg tcagtcccat gacggaagct gattctgtga tggcgttgag tttatcggcc
      781 atgtcagtga ttaaacgtat ccccgattcg taccccaaat caaacaattt cagacgcatc
      841 cagactaatc cgaggttcaa tataatccaa atgatagcgc ctaatgggtt accttccaga
      901 gccatgtagc cactgataga tcccatgata gttgggtaca atacccatag taaggaatct
      961 ccgataccgg acaaaggacc catcagggat gttttgattg aacggacagc tcccaaggat
     1021 tctgtccctt gttgctcttg tagcgctacg tcagcaccag cgactaaggg tcccatataa
     1081 ggggtgatgt tgaagtactc atattcaacc ctcattgctt ccacgtaatc atcgtcattt
     1141 ggataaattt tacgcaaggt tttcgccatg gaccaggcat agccggttcc ttgcaaggtt
     1201 ccgtagttaa tgttgttact gccgataaaa gaccagcgcc aagctgcatt acgacgatct
     1261 tttttgttta gttcctgtgt aagtcctttt gttctctcat tattcatcga agtcatctcc
     1321 ctcatcgtat gcagcatttt cagttgttac agccactttt tggctcgtta agttattata
     1381 gataaaaact gccagtgcta atccaacaat tgaaacggcc aagattggta attccatata
     1441 tgctgccatt ataaaaccgg ctaacaaagc ccaaatgtag cgttttgttg gcatatagcg
     1501 cattaacatg gctaccccga cgactggcag cataccccca gcgacgttta aaccatttgt
     1561 aatccattct ggaatccatt gggtggctgt ttcaattgct acttgaccaa aggctacgag
     1621 gataaccatc ggaatgaaag cttgcatcgc gacgaagaag acactgatga agttaatcca
     1681 ttgcatctta ctaaatttcc gttcctccaa atattgttta gatttttcgg aaacgaaaga
     1741 gttgattgtc cgcgaaaaga tgtccaagtt aatagccaaa ataccgactg gtaaaccgac
     1801 cgcgatggaa gttcctattt ctgctcctgt acgagcattc aagtaaatcg caacaacggt
     1861 tgccaaacca taatccggta ccgatgatcc acccaatgcg gctactccaa ggctcattaa
     1921 ttgaaacgtt ccagcgataa ccatggcgga ttgcaaatct cccatcacta aaccagctaa
     1981 aagaccaacc gttactggcc agtggttaat aatggtaatt ccccggttat caataagagt
     2041 aagcataggg attaaaataa ttaagatatt ctgtaaaaat tctggcatta taattcctcc
     2101 aatctcaatt tattaaataa atttgtctaa cggttcttct ttgtcggatg gagcaaattg
     2161 gtatgatacc ggaatgccct tatctgcaag ctgttgcaac tgttgcaatt cttctgggga
     2221 agccgcgaaa ttcttagcta actttttgcg gtcgtttcta tccagcataa tcccgatgtt
     2281 cactttcgga atggcgatgc cttgttcact caagtcgagt aacgttttaa cattattaac
     2341 taccaacaac acgttatgac tgtcatattt cccggcctta aaattggtaa tgccagtttc
     2401 tgtgtcaatc aaggacattc ctgtccctgc tggtttgctt agacgcatag ctgcctttct
     2461 tggttcatct tgggcaactt gatcgtccac taccatgata cgttttgctt ttaccgcact
     2521 ggcccattgt gtcacaacaa ttccgtgaac cagtcgttca tcaattcgag ctaatactaa
     2581 tcccattttt catttctcct tttcaatttc caaatatatc tgctagatgg tgttcgagtg
     2641 gtgctccctc tttacaaaat accggaatgg tattcagtgt tacagcaatt ttgtatatct
     2701 gaccaccagg gtaaactttt ttcgtgaaaa cttgaatcca attacaaccc tttggcaagt
     2761 agactggtat gtgtgttgcg ccttctgtca caaccggagc aatcaacaaa tcgctgccga
     2821 ataaataatg agtggtttct tcccaagatt tgggatcatc tggaaattct atgaacagac
     2881 tacgcataat tggtaaacct tttgtagcgc tctcattata tacatcccta atgtaatcct
     2941 tgagacgcgt tctgatatgg ataaagcgcg tcaatatgcc ttcaacttct tccccgaaag
     3001 accatatttc atttggtcca ccgcttgtcc ttaccccccc tcccgaattc cctatttttt
     3061 gggtatgagg ttggcgatca ccgtgcatcc gtagaatcgg actgaaaaca gcgaactgaa
     3121 accagcgaat catgagttct ttgaaaactg gggactgact gtttccacta tggaaacctc
     3181 cgatatcgct cgtccaccaa gtaatgccac ttatgcccat actaagaccg acttgaactt
     3241 gtcttcttag tgaatcgaat gaactttcga tatcccccga ccaagctaat gccccgtatt
     3301 tctgggcgtt aaaccaggca gaacggatta aggtaggttc tgtgttcgat ctctctggat
     3361 ttttctggat tgcctgtaga taatggagag ggtatttatt ggcatatttt tctaagctac
     3421 cctcgtatat aagatattcc ttgtgttggt acacatccat ttctggttcc gcttggtcag
     3481 cccaaaacaa ggaaattccc ttgctccggt aattttcgtc caagacctga cggaggaatg
     3541 tcttactctc tggatggaag aaatctaaca gagcttttcc ggcaaatagc gaatctttat
     3601 ttgtgaccga cctgatcaag agttgattat tccggtagct ctgataataa cgactctcct
     3661 cggttaccgt tggccataga gataccatca gttgggtgcc agtcttcttg atttcttccg
     3721 caaagtcctc gattccttcc caatagcgag aatcaaatgc gaaatcacca tctgaagtcc
     3781 aatgatagta atcaacaacc aaaacggaaa gagaaatgcc tcgttgattg taaccttcat
     3841 aaaccgttct taattcatcc aacgtctgat aacgcagctt gctttgccat aaccccagta
     3901 attccggttc caaccgaggc gtgcgcccag tcatttgcgt ataattttct atgatttgtt
     3961 tcggattttc tccgacggtc accaagtaat caataagatt cgtattgttg ctcttccata
     4021 ttttctggtt gtagccaaat gttacttccc cgattgccgc attattccag atgaaaccat
     4081 actgcttatc ggagagatag aatggtattg acgtctggga gttccgttgc attaactcgt
     4141 acgttccccc gtttttgtta aagttttctt cttgatatcc gcccatcccg tacaattttt
     4201 cattcggatc accttcaaaa cgaagagttg cttggtaggt gttctcgcca gaataggtga
     4261 attctcgagg tgaaatattt aacgatgagg tcggttcgtc cggaatcgga atgtgggtgt
     4321 caatgcctac agtacgtcgg acttgacttt gcttccgaga gtattcctcc aggattattg
     4381 tcccgttgtt gtaaaagacc aacttttctc catcgtatgt ggctgttaaa cggttgttgg
     4441 tcaagattaa ttggtccgac gtctctgtaa cggttgctgt agtcaagacg ttcttctcca
     4501 attcttctat cccttgtttt gggtcgaacc ctttgtggtt gggataacag gtaacgcgca
     4561 ccgtgtcgcg tccccatcct tccactttca ggcattgatt acggtccttc acgacgaagg
     4621 actgttcgga aacaatcact tcttttgatt taaagagcat agcttctttc ctcccaattg
     4681 acttgtcctt tatctcctct ctttatgggc tatttgccta acgattcaaa aagttcagag
     4741 gcggattctt taataaatac cgggatttgt tcaagaggtg ctgcgacata ataagtcgct
     4801 cccccagcat attctttacc ggtgaataag tgtcgccatt tttcaccgct tggtaagtaa
     4861 acctccctac ctctttcttt gtagttcata atcggagcaa ccaggatgtc agaaccaagc
     4921 atgtaggtat tatcgatttc ccaggatttt ttgtcctctg ggaattcgta gaataatgtc
     4981 cgcatgattg ggtatccgag ttcatgtgct tctgtcattg attctgccaa gtagtctttc
     5041 atccgttctc taatttccac gtagtccgtc atgatgtttt caacttcact gccgtaagac
     5101 caaatttcgt ttggtgcacc tgttggcatc gaaccaccac cagaattgga gagcggttta
     5161 ctatgtggca gacggtctcc atgcatgcgc aaaataggag aaaacgtgct gtactggaac
     5221 cagcgaacca tcaattcgcg gaattccgga tcttccggat taccaccatg gaagcccccg
     5281 atgtcagtcg tccaccatgg aataccggcc atccccatgt tcaatccgga gttgacttga
     5341 ttatgaaagg cttcgaaact ggaatcaata tccccagacc aagccaaagc accatacttc
     5401 tgagcacctg cccaagcacc tcgtaccaat gtgacaacgg attcatttga atccttattg
     5461 ccatcgtaaa ccatcttcaa gtatccgttt ggatacaagt tgccactttc caccgccgtt
     5521 ccggaatggt agcgataatt ttcgaaatca taaacagcat aacctggttc agccacatcc
     5581 acccagtagt aatcaatacc gatgtcttta taatttttct tgattaggtt ccagacataa
     5641 tcacgggctt caggattagt cgtatccatg aaaacagtgc ttccttggat ttgcatcgtc
     5701 aagcgaactc cgcgattcac tttaatcaaa tagccacctt ggagatattg ctcatagtta
     5761 taggcatctg attggacagt tggccaaatc gaaatcaatg gttcgacatt gtaatcttcc
     5821 ttgagtgttt tgaccagttt ttctggttct ggccaaaaat cgggatcaaa tcgatactcc
     5881 ccttgcttcg gccagtggaa atagtcaata gcaatagtcg atagctgaat gcctcgctgt
     5941 ttgtatcctt ctaccacttc taagacttct tcaggagtgc gatagcgtag tttactttgc
     6001 cataatccca gcagtttttt cggcatttca ggaacacgtc ccgtaacgct cgtgtattgc
     6061 tccgtaattt ctttaggtga atctccggct gtaatccaat aatcgatata atccgttccg
     6121 tacatttccc attcggtcat gttctttgca aaggttactt ttcctatacc aggatgattc
     6181 cacaagaaac cgtagttccg gcttgaaacg taaaagggaa ccgacatttg tgaattacgt
     6241 tgagccaatt cgagcattgt gttctttaaa tcaagataat tttgttggta ttgccccatc
     6301 ccatatattt tttcctgggc atgcggttca aatcggactt ttgttcgaaa gttcgacccg
     6361 tcgttatttg cacggtattc acgagacttc aactttaaag tggaattaaa atctttggta
     6421 aattcgactg tcccaacatc ctcactgcca tcgtcatgct taacggccct cagacgaatg
     6481 aattctttta agagaacttc atccttttca ttgtaaaacg tcaaacgatc tcgatgatca
     6541 aggacagctt ttattttacc gtttacaatc gtagcttgat tgtttttgta gtatgtctca
     6601 accagctttt catcataccc atgcagttca gttaatgcat tttcccgctc aacaaagtgt
     6661 tgatccaaaa acgaccggac tcgaagtgaa tgctggcccc atggttcaat aattagtaat
     6721 tcatcatcgt atttttttac gattcgattt gcttcaattg taatcatgat ttcctcctat
     6781 tagaattact tacaatatac taagcgcatg agacaaatct tttgtcgcac cgtacacctt
     6841 cgtatataag tcataaacag tattatattt ttctacgttt tccggaattg gttggacagt
     6901 agtaccatac cgaatgacct ggttggtaca agattcgaaa gaatcgaacc aacctagacc
     6961 gactgcagcc aagattgctg ctcccaaacc cggaccttct tccgtatcca ggctcacgac
     7021 ttccgtgtta aaaatatcag cctgcatctg taaccaagtc ttgtttttag cgccgccacc
     7081 cacactaatg atgcgcttga actcttcgcc ttttaaagaa gtcataagtt gttgagaatc
     7141 cttcaaggaa aatgttaccc cttccaaaac agcccgaatc aggtcatccc gggtatggtg
     7201 attactcaat ccgatgaagg acccacggat ttgactgtca acgtgtggtg tgcgttcccc
     7261 agaaatatac ggggtaaata gtagtccatt actgcctatc ggcacattag gaatgtttct
     7321 taagaattca tcgtatgatt gttccggtgc cagtaaattt ttgaaccatg agacgctatg
     7381 tccagcagcc aatgtgacgc ccatcgcgta gtaggtatct ggcaatacat ggttgaatag
     7441 atggactttt ccaccataac gcgtgtgatc ctcttcttcc agggccaaga acacaccgga
     7501 agtaccgata cttgctaaag cggtatggga atccgtcaat ccagatccca atgaggacac
     7561 agcattgtcg gctccaccgg taaaaattcg tacctctttt tcaaagccaa aggtcgcttt
     7621 gatttctggt ttcaatgttc caacacaagc ggatgattcc actaatgggg gtaagataac
     7681 ttcgggaatg ttatattttt ccagtatagg tttggaccac tctttcttag tcgtatccat
     7741 caataaagta ccggcggcat cggaataatc catgtggctg tggcccgtca ggtaatagcg
     7801 taagtaatcc ttgggtaaaa atattttacg taccttttcc cagatagcgg gctcatgttc
     7861 ctgtatccaa aggatttttg gtaaggtgaa tccttccaga gccctgttct tagtgattgc
     7921 aatgatttca ttcccaaatt catccataat gcgcttacat tgggccgaat ttcgaacatc
     7981 attccatagg atagccggtc gtaagacagt tcccgcttca tctgtaacga ccaaggaatg
     8041 catttgtccg gaaaaactaa ttcctgctag ttcagcctgg aaatcagaga cattgcaaga
     8101 aaatttttcg aacaatgcac cacaggctgt gagccaatca gcgggatttt gttcattata
     8161 gccccctttt gggctatgaa gggggtaatc acaagcttca atgtgccgaa ttaccccggt
     8221 ttcatccacc aacactcctt taagggcccc tgttcctaag tctagcccta atacataacc
     8281 catgttttac accccgactt tctatactaa ataatcatta atcagtgatt tgatgtattc
     8341 gatatgactt gattggtttg tcacatctga aagggaaagt gcgtactcac taagcgattc
     8401 aaaggtttct tggtcatcaa taattttttt accgattcct tgggtgaagc ttgcatattt
     8461 ttcttccata atttcttcga agaaacgttc ttctttcatc ttagcagcgg cacgcaaacc
     8521 acgtgcataa gtatccatcc ctacgatatg tgctaaaatc aaatcttcca tttcgaagga
     8581 agtccgacgt actttagcat cgaagttgat accacctggt gcaatccccc cattttccaa
     8641 gacctcatac attgccaaag tcgtggaata aatatctgta gggaattcgt ctgtatccca
     8701 ccccaacaac aaatcacctt ggttagcatc caacgaaccc aatgcgttgt aactgcgggc
     8761 tacattgatt tcgtgttcga acgtatgtcc tgccaacgta gcatggttgg cttcaaggtt
     8821 aagtttaaag tggtcttgta aaccatattt catgatgaac gccattgaag tagctgcatc
     8881 aaaatcatat tggtgttttg atggttcttt tggttttggt tcaatcagaa attggggttg
     8941 atggccaatt tttttcgtat aggcaatcgc cattttgtag aaacgagcca agttatccaa
     9001 ttcaaaatcc atgttggtat tcaataaact ttcatagcct tctcttccac cccaaaaaac
     9061 ataattttga ccatttaatt ttttgctaat atctaaacct ttttttactt gtgctgcagc
     9121 aatagcgtag acatcagcat tattagttgt tgctgctccg ttgacaaaac gaggatgtgt
     9181 aaacatatta gcagtgttcc aaagtaattt aatgcccgtt tgatccattt tttctttaat
     9241 cagatcggta atggtatcga tgttttggaa aaactcttcc aaggaatcac cttgaggagc
     9301 aatatcgaca tcgtggaaac agaaatattc cgctcccaat ttttctaaaa tttcaaagaa
     9361 agcctccaca cggtttttag ccgtttccat tgggttgttg ccttcccaat ttctttggta
     9421 agtacctgca ccgaatggat cggagccgtc tgccgtaatt gtgtgccagt atgctaccgc
     9481 aaaacggaga tgctctttca tcggcttgcc tagtatttct tcttcgggat tatagtagcg
     9541 aaaagcaaat ttatttttag tctgtttgcc ctcgtattgt atttttggta ccgcttcaaa
     9601 ataattcatt gctaattcca cctttcttag ttagttcgaa actcgtacta acctgatatg
     9661 attatattac ctgggtggga acagcttgtc aagcgctttc gttttttaaa aattaccttg
     9721 gttggttagg tgcgatttat attttttccc tgggacttcc tttagaatcc ttgacaagat
     9781 tgaaattata gtaaatgatt taataaaatc tatttcatat ttgaagtatt tcgaaattat
     9841 cttaattaaa ggagcagact aaattgaatg attacacacc gacacattac aatgagtcga
     9901 ccatcctcga attcatcatt caaaacgccg aggtttctcg atcaaaatta gcgaaattaa
     9961 caaacttaac gaagtcgtct atttccgata tcacaaaaac attgattgaa aaatcactcg
    10021 tctacgaatt aaacactgga atggcctccg tatcaggcgg tagaaaacct atttacttga
    10081 gttttaacaa cgaatgcgcc cttgttattg cgatagagat tgggaaggac tatatccata
    10141 gttctttgtc ctacatcaat ggggagttgg tcaataaatt gagttaccat tccctcttag
    10201 taaccaagga tacacttgtc atgttgttga caaaaataat ctccgattta acttcgaaaa
    10261 taccccagac cccttatggt attttaggaa ttgggatggc aatccatggc atcgtgaaag
    10321 atgatacagt cttgtttacg ccttattcag acattgattc cattcctttg aaagacctac
    10381 ttgccaaaga aattccctac ccaatcgtct taattaatga ggcaaacgcg ggtgctcttg
    10441 gggagtatac gtttaaaacc ccttcggaga atttggttaa catcaatatt caagacggga
    10501 ttggggctgg tatagttgag aatggtaagc tgtttatcgg ggcaaacggc acagcagggg
    10561 aaattggaca cactaccctt taccccgatg gcattctatg tccgtgcggg aatcatgggt
    10621 gtttggagca ttatgcttct acccgtattc tgttgaagaa gctgcttgat tcagatcata
    10681 aaccacatcc ggacatctca gtaatcgtta aaaaatggca ccagggggat gcacataccc
    10741 agaaaatact gaagcataat gcgaaattac tcagcatcgg tattaacaat attgtgctaa
    10801 tgtatgaccc agaggttgtc atcatcaaca gtgaaattta ccgaggcatt cccgaattga
    10861 ttgacgagat taagaagcac ttgactggta aaaacacaaa aaacttagct attaagaata
    10921 catctctaaa aggtaattct acactctatg gctgtgtttc catagttgcc cagtcctttc
    10981 tgaaaatcaa taatctgaaa tttacagtgt aaagtgatag aaaaatatcc tccttaaaac
    11041 ttcctatttt tcgggaggat atgattttat tagggtgtaa gtacttagca gcattttttt
    11101 cactaaatga tttcagctaa cgtttcccaa taacttttat catttgattt tttagaaaaa
    11161 gcataaattt tgttttttac ttccgtcata ttatgatctt ttatttgaat aacgctctga
    11221 ttgaaagtag ctatactgtc aatatgctga atatttaaat ttaacactaa tccttcccct
    11281 ttttttgtta aaaatgaaat ccgatcatta tctacgctat aagttccttc ttttaatttt
    11341 gaatgaggat ttgtttttag ctcgttatca taagcgttag caaataacgt aacggttata
    11401 ggtttatttc tcattttttc gtctctcctt tttggtctag agttttacaa cttttttctt
    11461 ggcgtttgtc aagaagccta tcttttgatc ttatttttta tttttttaat tatcttctgt
    11521 tctgttcata cagttgactc ctttcttatt tagtcctttt ttcaagtatg tccgtctgtc
    11581 gcctgttttg cccttatcat gagggattta gggaagtaaa gggcgagtgt agcgagtgca
    11641 ttttaccctt tacttcccta aagaacttat gatataaacc ggttggcgag agactgactt
    11701 gaaaatagga cgtgaataga agtaataggt tatgaacaaa cgggtaattt tttcaataaa
    11761 aaaacgaaca aaagcagcaa tctttttcga ttgctgcttt tgttcgtttc atctgtttta
    11821 attgcattta tttttgggta ttttg
//
LOCUS       DAPBAB010000066        11593 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPBAB010000066 DAPBAB010000000
VERSION     DAPBAB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.070"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..442)
                     /locus_tag="RD444_002274"
     CDS             complement(<1..442)
                     /locus_tag="RD444_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDT7549628.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="RD444_002275"
     CDS             complement(500..1552)
                     /locus_tag="RD444_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549629.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="RD444_002276"
     CDS             complement(1565..2503)
                     /locus_tag="RD444_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDT7549630.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2954..3604)
                     /locus_tag="RD444_002277"
     CDS             complement(2954..3604)
                     /locus_tag="RD444_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549631.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3704..4213)
                     /locus_tag="RD444_002278"
     CDS             complement(3704..4213)
                     /locus_tag="RD444_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDT7549632.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4576..4959)
                     /locus_tag="RD444_002279"
     CDS             complement(4576..4959)
                     /locus_tag="RD444_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7549633.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            complement(5200..5814)
                     /locus_tag="RD444_002280"
     CDS             complement(5200..5814)
                     /locus_tag="RD444_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549634.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            5958..6519
                     /locus_tag="RD444_002281"
                     /pseudo
     CDS             5958..6519
                     /locus_tag="RD444_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(6630..7604)
                     /gene="lacD"
                     /locus_tag="RD444_002282"
     CDS             complement(6630..7604)
                     /gene="lacD"
                     /locus_tag="RD444_002282"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDT7549635.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(7623..8078)
                     /locus_tag="RD444_002283"
     CDS             complement(7623..8078)
                     /locus_tag="RD444_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549636.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(8106..9551)
                     /locus_tag="RD444_002284"
     CDS             complement(8106..9551)
                     /locus_tag="RD444_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7549637.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(9548..10483)
                     /gene="pfkB"
                     /locus_tag="RD444_002285"
     CDS             complement(9548..10483)
                     /gene="pfkB"
                     /locus_tag="RD444_002285"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDT7549638.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10651..11409
                     /locus_tag="RD444_002286"
     CDS             10651..11409
                     /locus_tag="RD444_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7549639.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT         3632 a         2432 c         1800 g         3729 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     3061 tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     3121 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta ttgagttgat
     3181 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     3241 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcacttca cctaatgaat
     3301 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3361 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     3421 tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat
     3481 tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc
     3541 tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat
     3601 tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc
     3661 tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat
     3721 tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat
     3781 ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagagttctg
     3841 cctcagcaaa aacttctaaa atatcatcag tggtcataga caaggcgatt tttaattttt
     3901 ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg
     3961 gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa
     4021 cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct
     4081 gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa
     4141 aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat
     4201 cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt
     4261 cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgatac ggagctgggt
     4321 atcatgagtg aatgatattt ataaatcact ggatattgaa gtatattctg ttttctcaac
     4381 ttatttagtc agatttttta atgaagattt tttgagtaaa atttctataa ttcctactta
     4441 ttgaaaagta gtttgtagtg ctgcttctac ctctctcatc tgttctacaa attccaaata
     4501 acttgacgca gcttgcggaa tgaaaaaatg atatgtgaat atttctcctc ctagcccgct
     4561 taatatgcct attttttata cccatgattt gcaggaaggg gatcggaagt aggttgcaga
     4621 aagccgcctt tcataatctg tcggaaaccg gacatcaaaa cggattattt tacttgtcac
     4681 atttgtattt tcttgtgagg aagtgccaaa tacattattt acttgagtga cattctcatt
     4741 gtcctgtctg atattgcttc gtataaaagt accagatcca cgtctcgtat ataacaaacc
     4801 ttcatcaata aggagttgta tagctttgcg gatagtaaca cggcttgttt taaactctct
     4861 agccagactt tcttgctcag gtaatttaga accttcctga tattttccct caacgatcct
     4921 ctcttttaga gccaaggcta tttctttata ttttggcata ttttcccctc atttcattga
     4981 tcctacaaaa aaattagtat atatacaaat ttttgtaaag tatatatact aataaataca
     5041 aaaatatttt tattgttgga tacagaaatc tatttttaac tggggatatc tttgccagct
     5101 aatatctttg agactacacc tatcttcatc taaatggatc aattactcgc tttttccatt
     5161 gaccttcgtg gtacttctgc tttctgatag taaaaaacat tactgaatga tcttactata
     5221 taacatacag cggagatcta cttctttatt aaggatcttc atgacgttat ctttttcttt
     5281 tttccgttcg aaaccgcgtc gttcataaaa ttgatacgta cagccgctat ccgtgaacag
     5341 aaatacttct tttccttgct ctcggcgttc aaattctttt aataatttac taccgatccc
     5401 cttgatcttt aaatcagggt tggcagctaa aaagacaatt tctccatctg gggaatgttt
     5461 ttttaaatag tctgaaagca attcgtcatt ggtgtcgtca tacccgcctt tgctgtcttt
     5521 cacaaatgca ttttgtaaga aatcaaatag tttaacatat aacttttgac taaatgaatg
     5581 atgctttttt gattttcctt tgatgtctgc taataaaaca cctgccaact catcatccgc
     5641 atataaagca atgatttgtg tcgcgcgatt caattctaga taccaaaagt atcttccata
     5701 aagattcaaa aggagtttgt tcttggtata ccaatcaaag tgcatacctt taatcgcgaa
     5761 ttgaatggct tttttgtagt ctttcttttc cagttgctta aaggctaatt ccatcactta
     5821 ttctcctatt ctttaaatat tgatatgcta tacttatatt ataatagaca aggtgtcggt
     5881 tatctatgtt cagatagcgg tgaaattgtc ggataaccga cacaattcct tgattatgtt
     5941 gattaggagg ctgcaaaatg aaaaaagagg atcgccgcgt caaaaaaaca gaaaaagcgt
     6001 tagcagaagc attatcaaaa ttattagtag ataaaaaaat tcaaaacatc accatccgtg
     6061 aactgaccga aactgctgat cttcaccgtt cgacatttta tacacattat acggatatct
     6121 atgatcttta tgaaaaactg gaaaatagct ttttccaaga cttggatgaa atcatttcct
     6181 acgatacgac acatagctac gaggatctat acgctcaatt gatcgactat gtctatacga
     6241 atacgttcgt atacaagtta ttcgtcatca accacggaga tatccaattt caaaagagat
     6301 taaataatct gatcgaagaa aattatttaa aaatctggct acaagaagac aaaaaaaccg
     6361 tgatcacaga agaaatgcgt tacttcacca tctaccatat ccaagggtgc ctctccgttt
     6421 taaatcattg ggtagaaaag caattttgca tactctaaga aaaaaatgct gaccttcttg
     6481 cgcttattga acgacaacat agaacctatc atgccttaaa aacaaacgaa aaaggaagcg
     6541 tcgggacgct tccaattctt tatttaaaag ctcaaattga ttattaaaaa tcattaggtt
     6601 acaaacttaa ggttatagtt aaagctttct tacaatttaa tagctgttgc tgtttctttc
     6661 aaaacattat tcagctcttc gatgttcttg cgtccttgtg tctgcatcca ttctttggca
     6721 gcatattcac ctttagtaac gaatggttct acaccattcg cccaagttgc gcgcccacag
     6781 agtaccccat tgaatgtaga gccagcctct tttgcaaatt tcaaggtttc ttggaacaat
     6841 tcagcagtta ctcctgcgct caagaaaata aatggcaggt tggtagtctc ggattgttct
     6901 ttgaaataag cagcagcttc ttctttggta taagcaactt catttttagt gtaaccatcc
     6961 acatagttca tattaacagg aacttccact ttcaaaacgt caactccata gcgaggctta
     7021 gaaaattcct tcatcatttc atttacttta tgcggttttt tcaaggcata ttccttcgaa
     7081 gaagtatcag gatcgttggc atcataagaa accaattcta agaaaaatgg caaatcttca
     7141 gcaacacatt cagaaccaat ccgctccata aacgccttct tttggtcatt gatgtcatca
     7201 gattcatcta catcgtagta taacaagaac ttacaagcat cggccccttg ctcctttaga
     7261 cgttttacag accaaataga taaaatatcc ggtaaacgtc ctggagttga agcatcgtaa
     7321 ccagtttttt cataggccaa caacagacca gcatttttat cgcgaacttc ggctgccggc
     7381 aacccatatt ctggatctaa taagattgct gaagcatatg gagtcaattc agaagaaatg
     7441 atttttttga aattgatgat ttgttcatcg gttgctgatt tattttgaaa tttaccgatc
     7501 atttttttca aggcaccacg ttgatcgatt gctaacgcac tgatgatgcc actttcatta
     7561 cttagttgac gaatataagt tttctttttt tctgttaaag gcatgatttc actacttcct
     7621 ttttagtttt cttctaattt ttggtttaaa agttcagcta tttcttcagc agactgggct
     7681 tgtttcagac cttctgtcac atctttgtgc atcaaaagtc tggctacctg agataaaaat
     7741 tttagatggg ttgttccagc ctctgaatct ggaataaaca gagcgattac aaaatccgtt
     7801 ccttctccat ctaaactttt ccaatcgacc ttaccgttta accgaacaat cacaatatcg
     7861 gcatccttga tactagcatc tttggcatga gggatggcaa aaccatccat cataccagtc
     7921 gtgctttctt cttctctttc catcaatttt tcgtaaactg catctcggtc ttcactaatg
     7981 ctcaagacaa ttgactgatc tgcaaggaag ttcattacct catcttgcgt cgtaaaattt
     8041 ttattcataa aaactttatt tgttttgatc aaagtcattt gaaatccttc tttctagaat
     8101 aacctttagt tgttggttgc ttctttcatt tcaacagcag ctgcttctgc ttcacgtttt
     8161 tctacactac gtttttggat caaccctaaa attactccgc caacgattga accaagtaaa
     8221 atcgacccta cccatgcaac cttaccagta acaacaggta atactagaaa acctccatga
     8281 ggtgcgggaa cttggacacc aaacatatat gtcagtacag cagcaattga tgaacctagc
     8341 atcatagttg gcagaacacg cagcggatct ttagccgcaa atggaattgc tccttcggta
     8401 atgtgcgttg agcccaagat aaagttgact aaaccagcgt tgcgatcatt cgtgtcgaaa
     8461 tattttccaa aaaacagtgc ggcaaatcca gtaatcaatg ggggcgcgat acaagctgcc
     8521 gaaacccctg ccataaatgt atggttacct tgtgccaaca acgcagtccc tgtaacataa
     8581 gccgctttat tgactggacc tcccatatca aatgctgaca tacagccaac gataatccct
     8641 aaaattaatg gattagaatt ttggaatcct gccaagaaat tcatcattcc ttggttgact
     8701 gcttccattg gatttgctaa taaagacatg actacaccaa caaacaagac accgatcacc
     8761 ggatataaga agatcgcctt cagaccatcc aattgtttag gcaagccttt catgaccttt
     8821 tgcaatagta agataaagta gcctgctaag aaacctgcaa aaattccgcc taagaaaccg
     8881 gctccgccag aattggctaa cagtccgcca acaaacccaa caaccaaacc aggacgttta
     8941 gcgattcctt cagcgatata agctgctaaa ataggaacca tcattcccat tgccgcatca
     9001 ccaacaccct ttaacatagc ggcagtcgga ttgtaagtag gatcattggg atcaaaagag
     9061 tacacacccc agatggcaaa cgaaattgca ataattaccc cacctgccac tacaaacggc
     9121 agcatatgag aaacaccgtt catgagattt ttataaatac tgtgaccaat gcctgctgca
     9181 acccccatct ccgctttctc accagcagcg tcaactgttt tgttttctgc agtgattgaa
     9241 cctttaccag ccaacgcatc ttcgatcaat tggtcagcat ctttaatccc cttgctgaca
     9301 ggcacatcga ttacacgctt acctgcgaaa cgttctgctt gaacatcttt gtcagctgca
     9361 atgattacag cgtcagcgtt ttttatttct tctggtgtta attcattctc tatccctact
     9421 tgaccatggg tttcaacttt gatagatacc cctttgcgct tagctgcctg ctccaatgct
     9481 tcttgagcca tataagtgtg ggcaattcct gttggacatc ctgttgcggc aacaattttg
     9541 taagttgtca ttttttactc gctccctctt tgatttcgat ttgtttttcc aactctgcaa
     9601 catccgaaaa atctgttaat cctttacgaa aagcggttga acttccagct gctaaacttt
     9661 tcttcaaatt aatatctacc tgttcacctt ttaaatatcc tgcaagaaat gttcccaaca
     9721 aagtgtcacc agcacacgct gtattgacta ctttaccttt aggagaattc ccagaataaa
     9781 cgatatcctt atttaaaaat attcctccct ctcctcctag agaaaggaga acatttttcg
     9841 ctccatcggc aatcaagcgt tcagcataat gaatatatcc ttcacgatca ctaagctcga
     9901 cattatacca agattgcaat tcctcctcgt ttggttttaa taagaacggc tgatacggca
     9961 gacaatcaat tatttccgga tagcttgaat caataatcag atggacctta ttttcaaaac
    10021 aaatacgact gatatcaata agaatttcgg gtgagacccc tcgcggcaaa ctgccagaaa
    10081 cacacaaata atcgtcagct tgcagctttt gtatttgatt tagaaaagtt ttgatttgtt
    10141 cctgaggaat ctcaggtcct ttattaacca atttgaattc ttcattggtt ttattaactt
    10201 gtgtaaaaac gttgattcta gtcattcccg gtacgctaat aaaatcagtg ctaatatctt
    10261 tttgatgcaa ataatcctca atataattcc cggtaaaacc tgctttaaat cctaaagctg
    10321 tactatcgat acctaattgc ttcatgatca atgaaacatt cacacctttt ccattagctt
    10381 gtatatcatc atcgtttgtt cgattgactt gggatggttt catttcatct gtttcgataa
    10441 aaaggtcgat tgccaagttt aaagtacatg tataaactga catttacgct cctcctttct
    10501 tacatattca ttatgtatca agatcgaatg taaatgtttt cataaatgac taaattcatt
    10561 gaaaactttt acaaaatata ttaaataaac ttgaagtaaa ggaaactccc gctttacaat
    10621 agaattatcc taactaatcg gaggcaaaat atggatacaa aattaagtga atcggagaaa
    10681 tatctctggg attttattca aagtcacata ctcgaaatcc cctcctattc cattgtaaaa
    10741 ctaagtgaat atgccaacgt ttcaacagca accgtcgttc gtactatgaa gaaaaaagga
    10801 tatgaggggt ttacgtcttt taaacattcg ttaaaagaaa aagaaaatac aaatatcaat
    10861 tttgccgctc ttgataaagt tgatgaagga ataaagactg ctattcttaa aaatgagcaa
    10921 gaggtaatcc gcaccatcaa tatggtagaa agcgggaata ttgaagatgc tattcaacga
    10981 atcaaggctg ctagaagaat catgattttt gcccgaggtt tttcagaatt gattgctcag
    11041 gaaatgatgg tcaaatttca acttaccggt aaatattgtg agatgcatac tgatcccaat
    11101 atcataaaaa acattagtag aaaattatcc aaagatgaca tcgtgatctt tgtttcacta
    11161 aacggtgaaa caaaggaatt gactaccgca gctgaaaact gttataaagc tgagatcggg
    11221 acaattttgc ttactgctaa tcgtcattca agtttgatgg aacatatcga gattcctttt
    11281 gttggcttta aatcagaagg ctccttcttc ccagattatg aggtacgctc cagacttcca
    11341 ctttctatac tggctagaat cttacttgac tcctatgcac tgcgaacatc aggattcaca
    11401 cagcactaac tctatatctg taagactgta cctaaaatat atctgtgtga attaactaag
    11461 gttgtctaga aaaatattta atcgctattt gtattctcgt gctttctatc attgctcctt
    11521 ctataacttc ttagtttcaa gatagaatag tgcattgaga ctaccaggac tacttacgca
    11581 aatatataat aat
//
LOCUS       DAPBAB010000067        11514 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPBAB010000067 DAPBAB010000000
VERSION     DAPBAB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.071"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(79..2154)
                     /locus_tag="RD444_002287"
     CDS             complement(79..2154)
                     /locus_tag="RD444_002287"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDT7549640.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="RD444_002288"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="RD444_002288"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDT7549641.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="RD444_002289"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="RD444_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDT7549642.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="RD444_002290"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="RD444_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDT7549643.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="RD444_002291"
     CDS             complement(5326..5721)
                     /locus_tag="RD444_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDT7549644.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="RD444_002292"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="RD444_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDT7549645.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="RD444_002293"
     CDS             complement(6198..8390)
                     /locus_tag="RD444_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDT7549646.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="RD444_002294"
     CDS             complement(8414..9385)
                     /locus_tag="RD444_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDT7549647.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="RD444_002295"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="RD444_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDT7549648.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3111 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaa
//
LOCUS       DAPBAB010000068        11444 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPBAB010000068 DAPBAB010000000
VERSION     DAPBAB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.072"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            130..1389
                     /gene="lysA"
                     /locus_tag="RD444_002296"
     CDS             130..1389
                     /gene="lysA"
                     /locus_tag="RD444_002296"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDT7549649.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKERSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1376..2176
                     /gene="dapF"
                     /locus_tag="RD444_002297"
     CDS             1376..2176
                     /gene="dapF"
                     /locus_tag="RD444_002297"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDT7549650.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2179..3060
                     /locus_tag="RD444_002298"
     CDS             2179..3060
                     /locus_tag="RD444_002298"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDT7549651.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGKEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3057..3773
                     /locus_tag="RD444_002299"
     CDS             3057..3773
                     /locus_tag="RD444_002299"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDT7549652.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3779..4474
                     /gene="dapD"
                     /locus_tag="RD444_002300"
     CDS             3779..4474
                     /gene="dapD"
                     /locus_tag="RD444_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDT7549653.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAGTVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4490..5491
                     /locus_tag="RD444_002301"
     CDS             4490..5491
                     /locus_tag="RD444_002301"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDT7549654.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5488..6849
                     /locus_tag="RD444_002302"
     CDS             5488..6849
                     /locus_tag="RD444_002302"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDT7549655.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYSKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6809..7960
                     /locus_tag="RD444_002303"
     CDS             6809..7960
                     /locus_tag="RD444_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDT7549656.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFHYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSRSKEERVYNDEFIQSI"
     gene            7935..9104
                     /locus_tag="RD444_002304"
     CDS             7935..9104
                     /locus_tag="RD444_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7549657.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPKHVKQAGIAAIEEDFSHYTGMRGLEELREAACMFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9280..10659
                     /locus_tag="RD444_002305"
     CDS             9280..10659
                     /locus_tag="RD444_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7549658.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSVSDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11063..11444
                     /locus_tag="RD444_002306"
                     /pseudo
     CDS             11063..11444
                     /locus_tag="RD444_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3825 a         2003 c         2541 g         3075 t
ORIGIN      
        1 taaaaaaata tttcgaaata tactagacaa ttcagaaaat tttgtttaat attattccaa
       61 taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg
      121 aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt ggcagaaaag
      181 tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat gctgtcattc
      241 aagcgaggct ttgtatcgaa acgttttgat acaaaagtac tttacgcctc taaagcattc
      301 caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga tgttgtcagt
      361 ggtggggaaa tctataccgc tttgcaagct gatttcccta gagatactat ctacttccat
      421 ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt gttgcatatt
      481 gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg ctatttaaaa
      541 aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca tgaatatatc
      601 gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga gtgccaacaa
      661 gcccttcagt tgataaaaga aagaagctat ctagagttag aaggatttca tgctcatatc
      721 gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt gacgaattac
      781 ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg gattcgttat
      841 acacaaaatg atcagccaat cccagtcgaa gaggcactaa aaaaactgat tgaatatacg
      901 gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgattga accgggtaga
      961 agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa aaagacacca
     1021 cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc agctctgtat
     1081 caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac agagaaagtg
     1141 acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg
     1201 aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg atactcgatg
     1261 agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa tggccaatca
     1321 agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt ttcctatgat
     1381 ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg cagaagacac
     1441 agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata cagacggatt
     1501 gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg gcagccgcgc
     1561 gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc agaacatgat
     1621 gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta cggtgttgga
     1681 tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc cggaaaaaat
     1741 caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt
     1801 aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg aaaatgcgcg
     1861 tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt tcaaagaaaa
     1921 aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga
     1981 aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc
     2041 taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac aaggagactt
     2101 gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg aatggacaag
     2161 aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat
     2221 gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca tttagatgaa
     2281 caaacagacg gattattagt tttaggtaca acaggagaat cttctacgct cacacaatct
     2341 gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt gccggtgatt
     2401 gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca ttttgcttct
     2461 ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag tgatgcagga
     2521 ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat tttgtataat
     2581 gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt agcagaacat
     2641 cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat ggatgccgct
     2701 cggttgattg gaaaagaatt cttcctttac agtggaaatg atgatttgat cctaccagta
     2761 atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc taaaatagtc
     2821 catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga aaagcaatta
     2881 aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc tgtcaaagca
     2941 gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt ttctctttca
     3001 aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt tgaaaaatga
     3061 aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa
     3121 gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg cttgctttta
     3181 cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag aaaatccttt
     3241 cgtatagctt gtcttatcaa cttccgttag tcattggcac gacaggttat acacaatcac
     3301 agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt gctaattttt
     3361 cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct
     3421 ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca tctggtactg
     3481 caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta tacaactggc
     3541 aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca ggttctttac
     3601 ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc aaacatgaat
     3661 ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg ttgaaagatc
     3721 agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg taaaaaacgt
     3781 ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc ctgtgaaagt
     3841 ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg gtgaaggaag
     3901 cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag ctgaatcaga
     3961 aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc ctttgctaga
     4021 tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc aagtgtcaat
     4081 cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag taatcggtga
     4141 aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg
     4201 ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt
     4261 tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag gtgtccatat
     4321 tggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg cagcaggaac
     4381 tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa
     4441 tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat
     4501 agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca aagtgttaga
     4561 agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt cagccggtaa
     4621 agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa cttcatttga
     4681 aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt ttgcgccaat
     4741 cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg aagattcgga
     4801 tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc ggctgattgc
     4861 aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac aagaagcctt
     4921 tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg ggcaaaaagg
     4981 cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc cttataatat
     5041 tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata caaaagaaga
     5101 acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc cagtatcagc
     5161 aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag ttcaactgca
     5221 aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag ggattcaatt
     5281 agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag gaacagatat
     5341 ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt tgttctatac
     5401 gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt
     5461 aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc gaaattcggc
     5521 ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt tctttcagat
     5581 cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa
     5641 gtaacagact tactttattt gatttatgct taccttcagc atcatgttga ttggacaccc
     5701 ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc cctgcagtta
     5761 cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa gataactgaa
     5821 gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga gtatcttggt
     5881 taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa aatcgatcag
     5941 gaaaaaacag cacaggaaat cacaaaagca tttaaaagat attcgaaaat ggttgttcca
     6001 gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac
     6061 attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa ttggacggat
     6121 gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat catcgaggaa
     6181 atctctttta gagaactgag agagatggct tatatggggg ctaacgttct tcatgaagca
     6241 gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac caatgatcct
     6301 ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg actgacagga
     6361 atagctggac gcaaggattt tctttccatc actttattta aacgacatat gtccgatgaa
     6421 atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc aatcgaacat
     6481 attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat tgccgataaa
     6541 ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat cgtcgtgatt
     6601 gaagacttag ctttgatctc tgtcgttggc ggaccacata aagaattgat cggtttatct
     6661 ggaaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt atcacaagga
     6721 gcacaggagt tgaatctgat catcggtgta ccgaataacc aatatgaaac agtagtgaag
     6781 ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg cactccatca
     6841 aattcctgaa atcggatttc atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa
     6901 aaacagcggg gccgttatcc atgaaatcgg cgaaacagga ctactccttt attttgataa
     6961 tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag tcaaagaaga
     7021 aacgaatctc tcttttgctt ctaagcatga agggtatatg catgcatgtg gtcatgacgg
     7081 acatatggcg atgcttttag ggttgactga ctttataact aaacacattg aggatcttat
     7141 ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg cacaaagcgt
     7201 tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct ttcatctatg
     7261 gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg cacaaagcag
     7321 tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg aaaaaggaat
     7381 cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg accggcaatt
     7441 accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa cgatccgaaa
     7501 tgttttagct ggagaagtta ttgtatctgg aagtatccgt tcttacagtt cagatatgca
     7561 aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta cagctactat
     7621 ttcttttcac tataatgacg gctatccagc tgtaatcaac catagaggct tatatgcagc
     7681 actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag ccgaagattt
     7741 tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg gtgatacccc
     7801 tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg gtgtagaatg
     7861 gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca gatcaaaaga
     7921 ggagcgagta tataatgatg aatttatcca atcgatttag tcccaggctt tctagaatag
     7981 aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat gtgatcaaac
     8041 tcaccctggg agaaccggat tttccaactc ctaaacatgt caaacaagcc ggaatagcag
     8101 cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa ctgcgggaag
     8161 cggcttgtat gtttcagcaa cagcgttatg gattaactta tgatccgcaa acggaagttt
     8221 tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt ttggaagaag
     8281 gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta gtggaactag
     8341 ctggtgctga attgatcccg atagatactt ctgatacagg gtttgtctgt cagccggaac
     8401 aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt ttaaactatc
     8461 ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc gctgaggttt
     8521 taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg acttatagtg
     8581 gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata agcgggttat
     8641 ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa aaagccctta
     8701 ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg atgtcgcaaa
     8761 aagctgcagc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg aaagagcaat
     8821 atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt gaagtgaccc
     8881 agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa actgactctt
     8941 ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca ggaattgcat
     9001 ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg gaaaaattac
     9061 atgaggcctg caaacgaatc aaagactttt taacagaaca gtgattcata gattttatca
     9121 aaaaaacaat cgttaatttt gcggtatatt ctgcaaaatt aacgattgtt ttttaaattt
     9181 agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt
     9241 tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc
     9301 attgtaggtg catctcatgg agggcatcaa tcaatcttgg aactattgac acgatacggt
     9361 gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt
     9421 atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca
     9481 gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat
     9541 gccgataaaa aaacagtaac ggttactagc gtatcagata acgaaacaaa agaatatcct
     9601 tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca
     9661 gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa
     9721 ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa
     9781 gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga
     9841 cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa
     9901 aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt
     9961 gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt
    10021 gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc
    10081 caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca
    10141 cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt
    10201 agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc
    10261 ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg
    10321 aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg
    10381 ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc
    10441 tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca
    10501 ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca
    10561 gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc
    10621 gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag
    10681 aggttgtgac agaagtggtc agcttcaaga aataagaagg aattcacgaa aatcgtttct
    10741 caaatttttg tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgagt
    10801 cagaggttgt gaaaaagctg ccttgcatca agtaataaga acagccaagt aaaaacagct
    10861 tcttatgttt ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt
    10921 attttcaaaa aagggactgt gacacgattt gtgtatcgac ccccaaaagt tagaccaaaa
    10981 aatctaactt ttggaggtcg gtatttttta tggttaaata tacttttgaa cttaaattaa
    11041 agatcgttca tgattatttg gatggcaaag gcgattggac cgtaaaaatc cagatcaaga
    11101 gattgaagcg gaaatgctta aaattcggga gaagcacaag gattatggct gtttacggat
    11161 gaccaatgaa ctaagaaacc gcggtttctc tatcaacaaa aagaaagttc agcgcctgat
    11221 caagaagcta ggaataaaag taacggcata cacacctaaa tcacggcgct acaattctta
    11281 tcgaggacaa gtcggagcag tagcccaaaa tcggatccat cgtcgtttct acaccagtat
    11341 ctgccatcag aaaatcacga cggatacaac tgaatttaaa taccatgaag tggatgccaa
    11401 aggcattatc cgtcaaaaga aattgtatct agatcctttt atgg
//
LOCUS       DAPBAB010000069        11317 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPBAB010000069 DAPBAB010000000
VERSION     DAPBAB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.073"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..619
                     /locus_tag="RD444_002307"
     CDS             <1..619
                     /locus_tag="RD444_002307"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7549659.1"
                     /translation="VDIIGYDTYDKINHAYAFGGVAMTINTIQAYLDIPIDFFVTLNM
                     QGLEQIVDSIGGITLTPTATFTQDSYSFYQGEIINLDGKASLAYSRNRHADDSGDYGR
                     QARQREIIMAIIEKGTKVTTLATKIPSLIKSLQGNMTTNLSVDDMWTIINKYSTALGN
                     IEQVQLKGEGQLLDDGIYYEMINEESLEEVQNILQKQLDYHLNID"
     gene            669..1193
                     /locus_tag="RD444_002308"
     CDS             669..1193
                     /locus_tag="RD444_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7549660.1"
                     /translation="MQNDIRGKIKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSP
                     DINNLTLLSSLYEISIDDLLKEKKEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLV
                     LLLISLIGCLISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNI
                     FHWGGSTNVEYIEN"
     gene            complement(1190..1654)
                     /locus_tag="RD444_002309"
     CDS             complement(1190..1654)
                     /locus_tag="RD444_002309"
                     /inference="COORDINATES: protein motif:HMM:NF037267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5626 family protein"
                     /protein_id="HDT7549661.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            2568..4109
                     /gene="istA"
                     /locus_tag="RD444_002310"
     CDS             2568..4109
                     /gene="istA"
                     /locus_tag="RD444_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDT7549662.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            4109..4861
                     /gene="istB"
                     /locus_tag="RD444_002311"
     CDS             4109..4861
                     /gene="istB"
                     /locus_tag="RD444_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDT7549663.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWHEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(5044..5619)
                     /locus_tag="RD444_002312"
     CDS             complement(5044..5619)
                     /locus_tag="RD444_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549664.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            6407..6979
                     /locus_tag="RD444_002313"
     CDS             6407..6979
                     /locus_tag="RD444_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDT7549665.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVNEALKKYDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKYRHLW
                     SETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            7172..7735
                     /locus_tag="RD444_002314"
     CDS             7172..7735
                     /locus_tag="RD444_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7549666.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(8014..8301)
                     /locus_tag="RD444_002315"
     CDS             complement(8014..8301)
                     /locus_tag="RD444_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7549667.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(8291..8620)
                     /locus_tag="RD444_002316"
     CDS             complement(8291..8620)
                     /locus_tag="RD444_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7549668.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            8925..9254
                     /locus_tag="RD444_002317"
     CDS             8925..9254
                     /locus_tag="RD444_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7549669.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(9541..10026)
                     /gene="vanZ"
                     /locus_tag="RD444_002318"
     CDS             complement(9541..10026)
                     /gene="vanZ"
                     /locus_tag="RD444_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDT7549670.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10179..11090)
                     /locus_tag="RD444_002319"
     CDS             complement(10179..11090)
                     /locus_tag="RD444_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDT7549671.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3835 a         1984 c         1888 g         3610 t
ORIGIN      
        1 tgtcgatatt atcggttatg atacttacga caaaattaac catgcctatg cctttggtgg
       61 agtagccatg actataaata caatacaagc atacttagat atacctattg acttttttgt
      121 aacgttaaat atgcagggtc tagaacaaat tgttgattct attggaggaa taactcttac
      181 tccaactgcc acttttacac aagatagtta ttcattttat caaggcgaaa ttattaactt
      241 agatgggaag gcttccttag cgtactctcg aaatcgtcat gcagatgatt caggtgacta
      301 tgggagacaa gctcgtcaaa gggaaataat aatggcaatt atagagaaag gaactaaagt
      361 tactacacta gcaactaaga taccttcact cattaagagt cttcagggaa atatgactac
      421 taatctgtca gttgacgata tgtggactat tattaataaa tattccactg ctttaggaaa
      481 tatagagcaa gttcaattaa agggggaagg acaactactt gatgacggaa tatactatga
      541 aatgattaac gaagaatctt tagaagaagt tcagaatatc cttcaaaaac aactagatta
      601 tcacttgaat atagactaaa taatctattt cttaatatcc aataaataaa agtcattaat
      661 aggagaggat acagaatgac attaggggaa aaattaaaaa atcacggcat ataaaaggca
      721 tctctcaaag tgaagtagca aaatatttaa atatatctag acaatctatt tctaaatggg
      781 aaaatgatac tagttcccca gatataaaca acttaacttt attaagtagt ttatatgaaa
      841 tctctatcga tgatctttta aaagaaaaaa aagaagttaa aaaaaatata aaagagaata
      901 gtatagagat aatagaatct aaaaataaat taatttattt aaaaaataat acaaaccgag
      961 atgaagggtt agttttattg ctcatttcct taatcggctg tttgatttct cctcttggac
     1021 taattattgc gcctgttgtt ctgaaaagaa ataaagcaac aaacactttt tataaaggag
     1081 tgattgctgc ttcagtcatc tgttttacag taagcctgtt tgtctcctat ggacatttta
     1141 gtaatatctt tcattggggc ggctccacta acgtggaata tattgagaac taatacgtaa
     1201 tattaatgga attatctgtc agtgtagcat ctagataaga gttactagat aaaattccag
     1261 ctttccattc taatgtgtag ctagcttttt tgctggagac gagagacagt ctcgtgctag
     1321 taaaggatcc ggtcactgcg actgcagaag gggagtaggc cctaataaat tggttattcg
     1381 agactgtcac atagtaattt gccttccaca ttccaaattt agatccagat attttataat
     1441 ttccgttgtt gacggagaac agggagagcc cgggaacctc ttccacacga atcaagatag
     1501 gatttccttc ttcgtctacc tccatgaact cttgcgtgcc accttggatt aagttatagt
     1561 agactccatt atttgccaca tatgcttcta cagtgtttac ttcaaataaa aagaaagata
     1621 aacataaagt aacaaccaat aaaacttgtc tcaaaaaatc cctcctaaaa ttaatatact
     1681 catagtttaa tatatacggg tttaattagt aagagaacat tttgtgaaca ttcttatatt
     1741 gaaacggctc tctctacaaa tggacgcata tataagtgta tctgggttag taaacattca
     1801 ctgttaccaa ctctacttac taaacagata aaatatcaac gaaataaaaa aaccaaatca
     1861 actaattttt tactaataac gtagataata aaatcaaaaa gcaaatttga gcggttgcaa
     1921 ctataaagtg acctgatgta aaccataaga tgcttaatta agctatactt agatgagaca
     1981 ctatacttag gaagaactta gattagcata gcttcctaga acaaaaaaat acgagaggaa
     2041 gattctttta tgactaataa atatctatct gtatttactt ttttggttgg cttgtttttt
     2101 gttattaata gtgtagcaag ttctgatttt gatcttagta agattactct tttaactgta
     2161 ctaccaataa taataggtag tgtgttaatt cttttttctg tgacttccct ttttagacag
     2221 cgaggtaatt ccaaaaagca ttagcgaaaa agataaaagc agttttctct ttacccaaga
     2281 gagaactgct ttttatcatc tccacatttc ttgcttaatt aattacagct tcataaatac
     2341 tatcaatatg gctaataggg aaaccttcag taagggttac cctagatcct cctctagtgt
     2401 aaccagtgat tacggcatcg aaaatgtacc atctctgcaa taatttttac caggctcggc
     2461 aaagaaatgt accagtaagt gcaaagcagt gtaccacaaa tacctcttgt ataatgtagg
     2521 tgcacacctt agggtgtgac atttatttta caggaggtat ttttattatg tttcgttatc
     2581 gaaggatact tgaaatgcac agtgaaggaa ttagtattcg cagtattgca gcaagcacgg
     2641 ggaactcacg tcagaaaatt actgaggtta tccgaaaagc tgaggagaaa gggatagcga
     2701 gggcgttgtc ggaagaaatg accgatcaat ggctaggcga atttttgttt ccagaaaaac
     2761 aattagaagc caatggacgg cacctgcctg acttcgacaa gattcacaag gaattggcca
     2821 aaccaaacgt gaccttgacg cttcttcatc aggaatacgc agttgagtgt cgcatgagtg
     2881 ggaaaatccc ttacgcctat cgtacatttt gtgactatta caatgcgttc gcgcgaaagc
     2941 ataaagccac tatgcgcatc cgtcggaagc ctggtgaaat tatggaagtg gattgggctg
     3001 gatcgccagc ttatgtcatc gatcgagata cgggtgaaaa agtaaaggct tatgtctttg
     3061 tggcaacctt accgtgcagc caattgtctt atgcggaagc attcctaacg atgaaatcgg
     3121 tggcttggat tacggcacat aaccatgcat ttgaatattt tggcggttct accgacataa
     3181 ttgttcccga taatctaaaa acaggtgtaa aaaagaatga aggcatcgat gtcatcctta
     3241 atgaatcata tcgagagcta ggggaacatt acgggtccat cattttacct gcccgagttt
     3301 ctactcccaa ggataagccc agtgttgaag gcagcgtagg cgtcatctcc acttggatta
     3361 tagctgcctt acgcaatacg cattgtttta caatcgaaga tttaaatcgt gaaatcagaa
     3421 tgaaattaaa tgaattcaat gagcgaccgt tcactaagaa accaggatcc aggatcagag
     3481 ccttcgagga agaagaaaaa ttcgcactta atcctcttcc actggcacct tataagatgt
     3541 ctgtctggaa aaaagcaaag gttcaactaa actaccacgt ctcagttgaa agcatgtttt
     3601 attccgttcc atacgaatac attcaacaaa atatagatat tcgaatgaca gagaatctaa
     3661 ttgaactata ttttaattcc atgcgaatcg caacacataa acgtctgtac gggaaatttg
     3721 ggcagtattc gactgtcaga gatcatatgc cagataatca caaaatgtat gtggaccata
     3781 ccccagaatc ctcgatggaa tgggctgaaa ccatcggttc ctcaacagta aaggttgttc
     3841 aacacctctt agaccgttat caaaatgaaa aacaagcctt aaagtcgatc atgtctttga
     3901 agagctttgg taaaaagtat tctaaaaatg aaatagaaac ggcctgttca acagctttaa
     3961 atctggcttc tttgccttct gttaaaatta tccagaccat tctaaagggc aacaagcaga
     4021 agcgagcaac aagccaatca catattgaag acactaacat tgaaaagcat ggattcactc
     4081 gcggtgcgtc ctattttgga gggaaataat gatgttaaat gagcaaacag aagtaaaatt
     4141 agctgaattg aaactttcag gcatgcttga atcctatcgt gaacagatga caaataaaga
     4201 gtaccaatcc atgagtttcg aagaccgctt caacctcatg gttgatctgg agcattcccg
     4261 gcgaaaaaat aataaactac agcggttgat taaatctgca aactttagaa atgcctctgc
     4321 cagcattgag gatatcgaat accatccgga ccgtaattta gataagaacc tcattttaaa
     4381 actagccagt gggacttatt tggaagaaca ccataacatc attttgatgg gcgcttcagg
     4441 gaatgggaaa acgtatatcg ccaatgcctt cggaatacag gcatgccggc aatactacaa
     4501 cgtgaaatac atccgactcc ctgaactact agatgaatta accatctcga aaaatcaagc
     4561 agatggtagt tttcgaaaaa taatcaacag atataaaaaa gttcaattac tcatcatcga
     4621 tgaatggcta ttaaccaatc tttcgttaga aaattccacc tttctattgg aaataatcga
     4681 atcccgtctt aaaacagcat caactatttt ctgttctcaa ttccatccag aaggatggca
     4741 cgaaaagctc ggcaaccccc aaatcgctga tgccattcta gataggatca tacatgattc
     4801 ttatcaaatt ctggtagacg gtaaagtttc tatgcgtgaa cgttacggca tccgtaatta
     4861 aatgagtcat taaaataaat taagagccac tccatgattt tcatgagtgg ctcttagttg
     4921 accttgtggt cattattgtc gcttttatta tttgaaatgg taaccgcgtc gcacttactg
     4981 gtacatttct ttgcacttag tggtaaaaaa caatgctcta gtggtacatt ttcgatgccg
     5041 tattcaacca gcttgaataa aggcactaac acctgctgct gcatcatcgc aatggccaga
     5101 taaataatcc cctgtaacca tgcctaataa tactcctaac aatcccccag ctgtgccaac
     5161 taaggtacct cctactttca aagcaccagc attatacttc aactcgctac gcaatgcatt
     5221 taatcctctg taagaagtag catatgagtt aaccccacca ttcacaaatt tagttgtgaa
     5281 agcatcttgt gaattaacaa cagctgtcaa ttcagctcca tcatattttt cgactaaaga
     5341 aaaaaattgc tcttcactaa tagaattatc ttgactagaa gtagataata ctgattgtgt
     5401 agaaaataaa tcagggactg aaacagttga ttgattggaa gcaatcaatt cagctaactc
     5461 ttcttcaaac tgaatgtaaa gatcattttt tacaatttca taactaacag aatcttcctg
     5521 tttttcagaa gctttggcta ttgtaggagt aattaaggtc gatgtagtaa ttggcaataa
     5581 ggctaaaata gtaaataaat acttaaattt atttttcatt ttttatcctt ctttctattt
     5641 tttctacttt taataccaca acttgaaaaa taaaaaatct aagcaagagt aacatctctt
     5701 acgtaatgat tttgcaatag tttttcctcc tctcacaatc tattattatg tttttcgagc
     5761 tgtttcaaaa gcacttttct atttacatct gggaaactaa tcgttgcttt tataaaaaaa
     5821 gatgatagca gaaaaaattg aacttttctt tactataagg tatttataaa atcatcttca
     5881 taggtcaaag tagtagtatt aatctcaact ttgataagct ggtgtatatc aattttctct
     5941 ttaatatgta caatcattaa aaatagattt ttttactatt tatggtttca attaacaaaa
     6001 gaacctccac actaaatcac tataaattta ttatcacctt agttttttat gtgctcctag
     6061 catggaaatc ccatcaaaaa agtaaggttt gtattcaaat gtttttcgcc aacgagggta
     6121 tcttcttttt tacactcttt gctgtatcaa aatataactg aaattctaag tctcttctat
     6181 atcagtgatt atgtgattcg aaatatccat tccctcaaaa gaagttcata tagaaagtga
     6241 atagagagtg gctcctgtaa atatgatata gttaaactgg caaaataaaa tatgcttttt
     6301 caaaaagtaa tatttttgtt ttaattacga agaactactt ttaaatactg taagcacctt
     6361 ggcttgttag ttaactattc ttaaaatgag agggagacaa atatccatga ataaagctat
     6421 attttgggat ctgttaggta cgctgggagg ggatagtaga acactgatta atcaaaattt
     6481 caattttttt aaagacacaa taccggcctt aaaaaaagcg gcacaaagtc attatctaaa
     6541 tatcatcatt acgaatcaaa gtcatatttc acatggacgt attactatgg cagaatatac
     6601 tgatgcctta gagagcttaa taaatgagtt agagaaaaat gacattcaaa taacggatgt
     6661 atacacttgc cctcatgcta gaaaagacaa ttgtgattgc aagaagcctc aacctacatt
     6721 tgtcaatgaa gcattaaaaa aatacgattt aaatccaagt aagtgtttcg tagtaggcga
     6781 ttccggtaaa aacgatatga tgttggccaa aaatgctggt ataaacagtg ttttactttt
     6841 aacgggagag ggaattgata gtttaaccaa atatagacat ctgtggagtg aaacaaaacc
     6901 tacttttatt gctgaaaaca gtttagatgc tataaataaa atacatatgt atgcagatgg
     6961 agactcttct gattcatgat accagaagag tctccattta ttactaaaaa ccgtatcaaa
     7021 aacaatcgat tatgagacaa aatgaaatga cctcttttat taaataaaag aggtcatatt
     7081 ttgtatcata acttgttgca ttaactatgt atctataact cccttttatg atacaatcaa
     7141 gccagaatta aagtacgaaa ggaagaaaag catgagtgga tacatttatg gttatgcccg
     7201 agtgagtaca cgttcacaag aactaaatcg tcaattagac ttactcaatg aacaaaattg
     7261 taatgaaatc ctaactgaga aaatgacggg aaccaaagcg gttcgtcccc aactaaatcg
     7321 tttaaaagat aaaattcgtc cgggagatac ggtaattgta gaaagttttt ctcgattggg
     7381 tcgtagtaca aaggatttga ttgaccttgt tacctatttt gaagaacacc atgttaagct
     7441 ggtgagcttg aaagaaaact tcgataccag cacgcctcac ggacgcttaa tgatgactgt
     7501 cttccaagca tttagtcagt tcgaacgtga tttaattgtt gaacgtacca aagaaggttt
     7561 gaaaagtgca cgtgcgagag gtcgtaaagg tggccgtcca cgagtgaacc agaaagatgt
     7621 tgatcgagca gtaaaattgt ataaaagtca ggtttatagc gtaaaagaaa ttacggaaat
     7681 gactggtatt agcaaggcaa cactctaccg ctacttaaaa gacaataggg aataatctat
     7741 tttaatcttt aggaatctat aatatttatg ggagaattag agtgattttt ttattattac
     7801 taaaaaacaa tataaaataa cgcattttaa gccctctttg tgcataagga tgaagctttt
     7861 aatttgaaag aggcttaaaa tgcgttttaa ttttttctat ttttcataaa tatataggtt
     7921 agtggtttaa aattataggg tattacaact ctcagctgtt aaaaattatc ttttaaagtg
     7981 tcgtctatcc tctcaagttg aaacattggg ttcttaccta atctttttta attgttcata
     8041 aaaattttca tgcgttccac atagtaagat aggtatcact gcattatctt taatttcata
     8101 tgcaacgcga tatgtcgtac ctgcatactt gaatccaata gcccaaattc ctgataaatc
     8161 acccgttttt tgttctcccg aatgaggacg aactgctatc tcatcataaa ttatcgttaa
     8221 aaattgttct tttaatgttt tctcttttaa tttttttaga tactttatta cgtgtttaga
     8281 aggtagaatc tcataagcca atttctgctg ccaactcctc ttttgtcatc ggtttagaat
     8341 ttgcaacagt atcttcagca atagatttaa atgcagaagt taacgcattt tttctatttc
     8401 taaattcttt taagatctct tgtttgctat aaccttctga aagaatatca gccaaaatat
     8461 cttcactaaa atcaaaaaat tcttttttag gctctacaaa ctcataaagg ataccattct
     8521 ctacaagctt agcttctact tcaacaccat tgggcacatc gaattcttta gggaccgtta
     8581 acataataga atttccttgt tttcttgttt ttgtaatcat attatttcct ccattatttt
     8641 acatttcgta attacttagt aattacattg tacgctattt ttagtagaaa tacaaaataa
     8701 taaaactcct ttagaacaat tttcaaagga gttttatttg attaggaaat tatgtagaat
     8761 aataatatgt ttttaacttg gaatcaaaaa cgcacttgca agtaagttta cttcaataca
     8821 tgaaattgct tataatagtg tattggaatt aaggtagatt gacaaaagag tgatcaaata
     8881 ggtatacttc attcaaataa tcgtttgaat gtgaggtgag atgaatgaaa aaagtttgcc
     8941 aagtgactgt cgtacataaa gaaaaagtaa atggaataaa aaataaattg gatcaaaaag
     9001 atttttcaaa cttacttgtg ttagggaaat gtttcagcga tttttctaga attaaaattt
     9061 tctatgcttt agaaacctat aaggaaatgt gcgtctgtga tttagcagaa atacttactg
     9121 ctagtgtcgc tacaacgtca caccatttac gttttttaaa aaaacatgga atggcaaaat
     9181 cacgtcaaga tggaaaagta gtttattact ctttagcgaa tgaagacatc ctttccgcta
     9241 ttcgtgtttt ttaaagttat cagaaaatct atctatgaaa tcttagtttg ggggtaccgt
     9301 caggaaaatg cggatttaca acgttaagga aatttattaa agagaccaat tcctcagcac
     9361 taaaattgat aattgggctt tctttgttac tatagtgtta ggtaagcttt ttactttatc
     9421 accggttttc ccctcacttc acacctacgg gcgagtttcc ccacatcccg ttttccatct
     9481 aatccaattc attcaatatc tttatgtgta tgaaagatca gattgcttga tttagacatc
     9541 ttacacgtaa tttattctta aatgggtacg gtaaacgagc aataatacga gcaaaagtat
     9601 acctacaaaa ataataactc tgtctaattt tttttgattc atatgcttat tgcttaaacc
     9661 atataatttc agtccaagaa agcctccaac agtatttgta attacatctg ttatgtctgt
     9721 cgctccaata gcgaagataa attgaattat ttcaaaagta agacttaaaa ccagtacaaa
     9781 agcaaactta ggtaaaaatc cgatttcttt aaaattgaca ttcaaaagca agccaaatgg
     9841 aataaagatt ataacattat ctatcatctc tctgaaattc ccagtagcag taaatggagt
     9901 caagttaaga cttctttgat gataattaaa tactgataaa atattgtatt gtaatttgaa
     9961 taacactaac cagattagtg tcactaaata taaagctagc aatcctctag ataatatttt
    10021 tcccaatata atttcctcta attcttttgt ttaagttgta tcattagtat agcaatgtcc
    10081 tagttaacgt gggttttaaa ttaaaaagct aacgttaccc caaaagtgct agtacaataa
    10141 tgctgttcat taatttcgtt ggtttcattc cgccatcctt acctccttga attagtatgt
    10201 gttgatccgg gaaacactgt cacaattaca ccgtctatat tgtttcctga tatctcataa
    10261 cgaagattag tcggcacatg aatggtggta tttttagtaa caggataata aaagatctca
    10321 tatttttccc catttacact aacagaaatg gttttttctt cttttaggta atccatatat
    10381 tcctcgagaa cgaaattctt ttctttcata atcgcactat gtggtaaacc aacatagcga
    10441 atatgccatg gttcatattg aattcctgtt aactctgttt tgtcctctgg ataacgtaaa
    10501 atgaacccgt atttccaagc attttcttct atccactttc cttcaggggc tcgttccatt
    10561 ttcgtcaagc ttgatcctac atctagtgat aaacctgaat tatgctcact ataacctgct
    10621 ggtaaggcat actcagcccc catttcttgg taaagcacac tttgctcatc aaagtctcga
    10681 tagccactat taataataaa atgactaacg ccacccttta cagcatcatt gaccatctct
    10741 gaaaattttt gtgctatttc ttttgacata taaatattac tatcaagcaa cccgtatcca
    10801 tttattaatt cgtcatgttt agataaattc acgatatctg acttcacact ttcttggcga
    10861 acaggatatt tactattgat taatagcaga tttccttgat aaacctgttc ttctgtaatt
    10921 gttttctctt gggtattttc agaagtccca ctattttcta aatgttcttt gggattttga
    10981 tcataatttt gaaattcgac tttttcctga gaaaacagtg cttcattaac gtagtcataa
    11041 cctaagtata ttaagaataa caataacaat aaaaaaaaca acttcttcat tttcagtctc
    11101 ctccttaact atatagttgg aagtatgaaa tatttctaga atcttcccct taaacggcac
    11161 aatctcgttt gagattgtgc cgttttgccc cgtagaaggc actctcttcg ggttttcggt
    11221 ctgtactgaa atcaaggtat tattgggaat cccagcttaa atcatagata ccgtaaggga
    11281 ttttattctt tatttaaaac tttgcaacag aaccgtt
//
LOCUS       DAPBAB010000070        11254 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPBAB010000070 DAPBAB010000000
VERSION     DAPBAB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.075"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="RD444_002320"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="RD444_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDT7549672.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="RD444_002321"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="RD444_002321"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDT7549673.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="RD444_002322"
     CDS             complement(2169..2906)
                     /locus_tag="RD444_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDT7549674.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="RD444_002323"
     CDS             complement(2997..3524)
                     /locus_tag="RD444_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549675.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="RD444_002324"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="RD444_002324"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDT7549676.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="RD444_002325"
     CDS             complement(4401..4886)
                     /locus_tag="RD444_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDT7549677.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="RD444_002326"
     CDS             complement(5039..5236)
                     /locus_tag="RD444_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549678.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="RD444_002327"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="RD444_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDT7549679.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="RD444_002328"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="RD444_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDT7549680.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="RD444_002329"
     CDS             9211..9423
                     /locus_tag="RD444_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDT7549681.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="RD444_002330"
     CDS             9425..11104
                     /locus_tag="RD444_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDT7549682.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DAPBAB010000071        11150 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPBAB010000071 DAPBAB010000000
VERSION     DAPBAB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.076"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="RD444_002331"
     CDS             6..305
                     /locus_tag="RD444_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549683.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="RD444_002332"
     CDS             378..1739
                     /locus_tag="RD444_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDT7549684.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="RD444_002333"
     CDS             1761..2021
                     /locus_tag="RD444_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549685.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="RD444_002334"
     CDS             2035..2787
                     /locus_tag="RD444_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDT7549686.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="RD444_002335"
     CDS             complement(2881..3486)
                     /locus_tag="RD444_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7549687.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="RD444_002336"
     CDS             complement(3542..4516)
                     /locus_tag="RD444_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDT7549688.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="RD444_002337"
     CDS             complement(4894..5613)
                     /locus_tag="RD444_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7549689.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="RD444_002338"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="RD444_002338"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDT7549690.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="RD444_002339"
     CDS             7164..8561
                     /locus_tag="RD444_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7549691.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="RD444_002340"
     CDS             8642..9082
                     /locus_tag="RD444_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7549692.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="RD444_002341"
     CDS             9229..9555
                     /locus_tag="RD444_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7549693.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="RD444_002342"
     CDS             9556..10305
                     /locus_tag="RD444_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549694.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="RD444_002343"
     CDS             complement(10331..10741)
                     /locus_tag="RD444_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDT7549695.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DAPBAB010000072        11134 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPBAB010000072 DAPBAB010000000
VERSION     DAPBAB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.077"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            117..353
                     /gene="secG"
                     /locus_tag="RD444_002344"
     CDS             117..353
                     /gene="secG"
                     /locus_tag="RD444_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDT7549696.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            456..1211
                     /locus_tag="RD444_002345"
     CDS             456..1211
                     /locus_tag="RD444_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7549697.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            1214..3571
                     /gene="rnr"
                     /locus_tag="RD444_002346"
     CDS             1214..3571
                     /gene="rnr"
                     /locus_tag="RD444_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDT7549698.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            3593..4057
                     /gene="smpB"
                     /locus_tag="RD444_002347"
     CDS             3593..4057
                     /gene="smpB"
                     /locus_tag="RD444_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDT7549699.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            4359..5078
                     /gene="atpB"
                     /locus_tag="RD444_002348"
     CDS             4359..5078
                     /gene="atpB"
                     /locus_tag="RD444_002348"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDT7549700.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            5131..5346
                     /gene="atpE"
                     /locus_tag="RD444_002349"
     CDS             5131..5346
                     /gene="atpE"
                     /locus_tag="RD444_002349"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDT7549701.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            5437..5961
                     /gene="atpF"
                     /locus_tag="RD444_002350"
     CDS             5437..5961
                     /gene="atpF"
                     /locus_tag="RD444_002350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDT7549702.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            5948..6490
                     /locus_tag="RD444_002351"
     CDS             5948..6490
                     /locus_tag="RD444_002351"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDT7549703.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            6513..8069
                     /locus_tag="RD444_002352"
     CDS             6513..8069
                     /locus_tag="RD444_002352"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDT7549704.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            8083..8985
                     /locus_tag="RD444_002353"
     CDS             8083..8985
                     /locus_tag="RD444_002353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDT7549705.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            9008..10414
                     /gene="atpD"
                     /locus_tag="RD444_002354"
     CDS             9008..10414
                     /gene="atpD"
                     /locus_tag="RD444_002354"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDT7549706.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            10428..10850
                     /locus_tag="RD444_002355"
     CDS             10428..10850
                     /locus_tag="RD444_002355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDT7549707.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         3700 a         1966 c         2450 g         3018 t
ORIGIN      
        1 aataacagct gaaagttttg aactttcagc tgttattcca tttatataga gttgtcacgg
       61 acttttatct atgataaagt taaagagacg atatcttttg ggaaggaggc tgcgatatgt
      121 ataatttaat tttgggaatc gtgatcgttt tatcgattgt tatggtcatt gcgattatga
      181 tgcagccaag caagcaaaat agtgcagcga gtgcatttac cggcggtgcg gatcaactat
      241 tcggcaaaca aaaagctcgc ggctttgaag ctgtcatgca gcgatcaact gcagttatgg
      301 gagctatctg gatgatctta ttatttgtcc tagcatattt atcttcgaag tagttgaata
      361 agcctctcgc aaaacggcgg gcggcttttt ttattgcaaa gagaacgctt aagaagaagt
      421 tatgaaagaa actatggtaa aatgaggcga aagaggtgag acgagtgaga caattaccaa
      481 aaccccttta tgcaaaacat ggaaagcgag cggttttgct gcttcacgct tactcaggaa
      541 gtccaaacga tgttcgaatg ctcgcacgat ttttggaaaa agcagattat acagtttatg
      601 cgccgctttt taaaggacac ggaacaatgg agccatatga tatcttgcaa gaaaaagcag
      661 aaagctggtg ggcagacact aaaaaagcca tccatttttt acagtctgag cagttttctg
      721 atattgccgt attaggatta tcaatgggcg gcatctttgc tgtacgagca ctagaagaag
      781 aatcagtgat tggcggcggt tttttctgtt cgcctttgtc tcctgtaaaa acaaacgtgc
      841 ctgaaaactt tgaaaaatac gtacgtcaag tgctgaagac agcaggtaaa tcagagaaag
      901 aaatcaatga aaaagcagtt gcttaccgct cgttagctga acaacaatta atggatatac
      961 aagatcaggc agcaatcgta gaaagtaggt tgtcagatat ccaacagccg atatttttag
     1021 ctcaagctgg gaaagatgaa atgatcgacc ctaatggcgt atttgaaact gcacgcaagt
     1081 tgagccgtca gcgagtgaca cttcaatggt atcctgaaag tggtcatgtg attacagttg
     1141 gtacagcaag acgcgaatta gaaaaagacg tcttggaatt tttagaaaaa ttaccttgga
     1201 atgaggaata aatatgacaa aagaaacgat aaaagagaaa attctctttt tcatggaaag
     1261 cagtcgcaaa cgcagttttt ctatggaaga aatcgctgaa ggtttagggt tccaaaaaag
     1321 cgatgatttt aaattactag tacaaacgat tgctcaaatg gaaagagaac agagtatagt
     1381 tttcaataaa aaaggaaaag tgaaacttcc tttaaaacca atcttgatcg aaggaacatt
     1441 tcgagcaaat gaacgaggat ttggatttgt aacgattgat cccgaagaag atgacatcta
     1501 tatcccaaaa gaagcgactg gttttgcaat ggatggagac accgtagcaa tcgatatcat
     1561 gaagcgagca aatacagata tggatcgcgg agcagagggg aaagtagtcg agattcgaga
     1621 ccgggcgact actcagttag caggggaatt cgttgcttat tcagaagaag aaatacaaga
     1681 aacagatctt tacggcgttg ttatcccgaa agatagaaag ctcaaccaat ttaaagtata
     1741 tgcagcagca gagggagttc gtccagttga cggaagtatt gtgctgatcg aactgaccca
     1801 ttaccctgaa aaagggtatg cgactagttt agaagggata atcaagcaag tgattggtca
     1861 taagaatgac cctggcatgg atatcctctc aattgtcgtg gctaatggta tccctacgaa
     1921 atttcctgat gatgtattgt ctgaagcaga tgaagttcca gatcaaatca gcgaaaagga
     1981 tataactgga cgtagagatt taagaaatca gctcattgtt acgattgatg gagaagatgc
     2041 aaaagatcta gatgatgcag ttacggttaa aaaattagat aacggtaatt attttcttgg
     2101 agtacacatt gcagatgttt cttactatgt gacacaaggc agtcaattgg acagagaagc
     2161 atatgaaaga gggacaagtg tatatttgac tgatcgagta attccgatga tcccgcaacg
     2221 tttatcgaat ggtatctgtt cactgaatcc ccatgttcca aggctgacca tgagttgcga
     2281 aatggagatt gatccaaatg gacaagtagt ctctcatgat attttcccaa gtgtgatcca
     2341 aacaacagaa cggatgacct acacagctgt caatcaaatc ttagaagatc aagacgaaga
     2401 aacgatgcaa cgctatgctc agctcgtacc tatgttccaa gaaatgggtg aattgcaccg
     2461 aatccttgaa acgatgcgaa tcaaacgtgg cgcgatttct tttgaggatc gcgaagcgaa
     2521 aatccttgtt gatgctgaag gacacccaaa tgacatccag ctaagaagca gaggaattgg
     2581 tgaacgcttg atcgaatctt tcatgcttgc ggccaatgaa acggtagctg aacattataa
     2641 taaattaaat ctgccgttta tttaccgaat acatgaacag ccaaaagaag aaaaaatgca
     2701 gcgcttcttt gattttgctt cagctttggg cattctggtc cgaggaacga aaggaacgat
     2761 cacaccaaaa gatttacagc atgtgatcga aggagtagaa gacaaaccag aagctgcagt
     2821 tatcaatact atgttgctaa gaagcatgca acaagcacgc tattcagaag acaattttgg
     2881 gcattatggc ttagcggcag attactacac acactttaca tctcctattc gacgctatcc
     2941 cgatctaatc gtccatcgtc tgatccgttc ttacagtgaa gataaaagtg aaagcaatca
     3001 aagttcgtgg gctgcggaac ttcctgaaat tgcagatcat agttctaaga tggaacgtcg
     3061 tgcagtagaa acagaacgtg aagtagattc catgaagaaa gcagaattca tgatggacaa
     3121 agtgggagaa gaattcgatg ggatcatcag ctctgttgtg aaattcggcc tgtttatcga
     3181 actgccgaat acagtagagg gattgatcca tatcaatgaa ttgaaacagg actacttcca
     3241 ttttgttgaa aatcatttag ctcttgtagg tgaaagaact ggtctgactt ttaaaatcgg
     3301 acagaaagtg cgagtaaaag tagtcaaagc tgatccagat gaacgagcag tagattttga
     3361 acttgtttcc gcagaagagg ttacagcgtt ggaacggcca aaaacacgaa atggaaaatc
     3421 aggaaaacgc gaaggaaaac aagcaggaaa acgcccagaa agaaatggca agaagaacaa
     3481 acgatatagc gatagtaagc aaacgttcgg taagaaaaaa gggaagaagc cattttacaa
     3541 aggtgttaaa aaaaagaaaa agaaaaaata aaatgagttg gaggaaacag ctatgccaaa
     3601 aggcgaggga aaattaattg cacaaaacaa gaaagctcgc catgattatt cgatcatcga
     3661 cacgatggaa gcagggatgg tcttgcaagg aaccgagatc aagtcgatac gaaacagccg
     3721 gatcaatcta aaagatggat ttattcgcgt ccgcaacgga gaagctttct tgcataatgt
     3781 tcatatcagt ccttatgaac aaggaaatat ttttaatcat gatccgttgc gcacgagaaa
     3841 gttattatta cacaaaaaac aaatcatccg gcttgaaaat gaattgaaaa atactggaat
     3901 cactgttgtt cctttaaaag tctatattcg taacggctat gccaaggtat tgattggtct
     3961 ggcgaaaggg aaaaaatctt atgataaacg ggaagatttg aaacgaaaag atatcgatcg
     4021 acaaattgat cgaacattaa aaaatttctc tagataattt cattatcctt tttcgcatat
     4081 tcggtgcttt tgcttttact taatgataaa atttgttagc atttaatctg ataaaacatg
     4141 attatcatag gttttttctt agatcaggag caccgtgtat aaagaaaaat tcgcttcgtt
     4201 tttgactgaa ataaaaaagt gtctaatttt catgaaaatg ttttgaattt tctgtttcat
     4261 ggtggtatga taccgtgtgg ttagaaaccg attggaaaaa accagatacg cacatgaatt
     4321 gattttatgt gaatttcggg gaaaagaggt gaaatggatt ggatgaacgc tcgctaacgt
     4381 tccacattgg accaatttgg tttgatggta cagtttgtat aatggtgtta ctgacctgtt
     4441 tgattgtttt cttcttagct tactttttta caagaaatct taagttgaaa ccaacaggca
     4501 aacaaaatgc gatagaatgg gtcattgatt ttagtcgagg aatcgtaaca gataatctgc
     4561 ctagaaaaga attgagcaat ttccatttgt tggcatttac attgttttta tttgtttttg
     4621 ttgcaaacaa catcggttta gtaacaaaaa tcgttttacc taacgaaaca acactttgga
     4681 aaagcccgac agcagacccg tttgttactt taactttggc atttataatg atcttactta
     4741 ctcacttatt tggggtgaaa aagttaggct tcaaaggata tttcgtaaat tcgttcttga
     4801 aaccatatag ttttatgttt ccgatgaaac tgattgaaga atttacaaac ttattaacac
     4861 ttgctttacg tctttatggg aatatttatg cgggggaagt tttgctaacc ttgattgcaa
     4921 aaatggtaat aagcttaggc tggttctcat tgccgttagc aattccatta gagatggtct
     4981 ggatcgcatt ctccttgttt atcggaagca tccaagcatt tgtcttcgtt actttatcta
     5041 tggtttatat gagccataag attgaagtgg aagaataata ttcgttttaa aaacaaacta
     5101 ctatttatct aatttaggag gaaaacaatt atgaattata tcgcagcagc aatcgcaatc
     5161 atgggagcag caatcggagc cggttatggt aacggtcaag ttatctctaa aacaatcgaa
     5221 tcaatggctc gccaacctga aatgtctggt caattccgta caacaatgtt tatcggggta
     5281 gctctagtcg aagctgttcc tatcttaggg gtagttgtcg ccttgattct agtttttggt
     5341 gtttaattga atcagtatta aggcagaaac aatttggttt gttactgctt ttttacatga
     5401 aaactagaag tacttcaaag aaaggaaggc tcgcctatgc tgaatcaatt ggcaattgca
     5461 gaagttggca atgcgatgtt aggaaatgtc atcgttgtca gcggctcatt tttgatacta
     5521 ctggctctct tgaagcattt tgcttgggga ccaatcagcg atattttgaa aaaacgtgaa
     5581 gacaagatcg ccaatgattt ggattctgca gaacaatctc gcatcaactc agcgaaaatg
     5641 gaacaagaac gcgaacaaca attgttagcc tctcgttctg atgcagctga tatcatcaaa
     5701 aatgcgaaag aaagtggaga attaagccgc caaaatattt tgaaggatgc tcaagaagaa
     5761 gcagctcgtc taaaaagtaa agcccaagct gatatcactg tagaacgtga ttcagcgctg
     5821 aactctgtaa aagacgatgt tgcagaacta tctcttcaaa tcgcggaaaa aatcttgaac
     5881 aaagaattat ctccagaaat gcacgaatca ttaatcaatc aatatatcga aggtctaggt
     5941 tcttcaaatg aaactagata aatatacagt aggaagacgt tacgggaaag ctttatttga
     6001 actggcgatc gattcaaatc aagcagaaga aatctatcaa gatctcgtaa gcttacgtca
     6061 aatctatgaa caagtacctg gattaggtaa tatgttaagt gatgttcgtc tagaacctca
     6121 tgaaaaacga gaaatcatgg acaagcttgt tagtggctat gatggaatcg ttaaaaactt
     6181 tttagaagtt gtttatgcgt ataaccgaat ggatgatctt ttgttcatga tcgaagaata
     6241 tgaacgtcga tatgatgaac atcaaggttt gctgttggga agtgtcacaa cagcagttcc
     6301 actttcagac gaacaacgaa tacaattaga aaagaatgtt gcaaaaacaa tgaactatca
     6361 atcagtcgaa ttaaaacaaa tcgttgattc ttctattatt ggtggcgcga ttgttgaagc
     6421 taaccatcga gtaatcgacg gaagcattcg cacgcaatta gaaaatatgc gtaatcaatt
     6481 aaatagatag tacaaagagg taggtgaatc gaatggccat caaagcagaa gaaattagtg
     6541 ccttgattaa agaacaaatt gaaaattatc aaaacgtgct ttcagtcgaa gaaatcggta
     6601 ccgtaactta tgtaggtgac gggatcgctc gtgcacatgg tttagaaaac gcgatgagtg
     6661 gtgagttgct tgaattttca aatggctcat acggaatggc gcaaaactta gaatcaaatg
     6721 atgtaggtat cattatcctt ggcgatttcg agtccattcg tgaaggagat aaagtaaaac
     6781 gaacaggaaa gatcatggaa gttccagttg gagaggcctt gatcggacgg gtagttaatc
     6841 cgctaggtca gccaatcgac ggactaggtg aaatcgttac tgacaaagct cgtccagtag
     6901 aagcaatggc accaggcgtt atgcaacgta aatctgttaa cgaaccaatg caaacaggtc
     6961 taaaagcaat cgacgctctt gtaccaatcg gacgtggcca acgtgaatta gttatcggtg
     7021 accgtaaaac agggaaaact tctattgcga tcgatacgat catcaaccaa aaaggtcaag
     7081 atatgatctg tatctatgta gcaatcggac aaaaagattc tacagttcgt acacaagttg
     7141 aaacattgaa aaaatatggc gcaatggatt acacaatcgt tgtgaatgcc ggtgcgtctc
     7201 aaccagcgcc gttgctttat atcgcaccat atgctggtac tgcaatgggt gaagagttca
     7261 tgtacaacgg taaacatgtc ttgatcattt ttgatgatct ttcaaaacaa gctgttgctt
     7321 atcgtgaact ttccttgcta cttcgccgtc caccaggtcg tgaagcttat ccaggggatg
     7381 ttttctactt acactcacgc ttattggaac gtgcagcaaa attaagtgat gatttaggtg
     7441 gcggttcaat gactgcctta ccatttgttg aaacacaagc tggagatatc tctgcttata
     7501 tcccaacaaa cgttatttcg atcactgatg gacaaatctt cttagaaagc gacttgttct
     7561 acgcaggtac tcgtccagcg gttgatgccg gactatctgt atctcgtgta gggggttctg
     7621 cgcaaatcaa agcaatgaaa aaagttgctg ggactttacg tctagactta gcaagttacc
     7681 gtgaattaga agcatttaca caattcggtt ctgacttgga tgcagcaaca caagcaaaac
     7741 taaatcgtgg acgccgtaca gtagaaatct tgaaacaaaa attgcatgca ccgctagctg
     7801 ttgaaaaaca agtagtgatt ttgtatgctt tgacacatgg attcttagat agtatccctg
     7861 tagacagtat tttagacttc gaacatgaat tattcgaata tctggatacc aatcattcag
     7921 atatctttga aacaatccgt acaacaaaag atcttccaga agaagaagcg ttgaacagtg
     7981 cgattcagga atataaagac atgttcttag ctacaaaagg aaataattcc tctacagaag
     8041 ataaattgaa atccattcaa aatgcatagt gaggtgagat gaatgggtgc ttcattaaat
     8101 gaaatcaaga cacgtatcgc ttcaacgaaa aaaacgagcc aaatcacgcg ggcaatgcag
     8161 atggtgtctg cttctaagtt gacaaaatct gaagcttctt cacagaagtt tcaggtttat
     8221 gcaacaaaag taagagaaat cgttacgcat ttgactgcta ctcagttaaa tgatattgcc
     8281 tcagaaaatc ctagagggga tatcaattac aacagcatgc tgctcagccg tcctgttaag
     8341 aaaactggtt atatcgtcat cactgcagat ggcggtttag tcggtggata taacagctcg
     8401 atattgaaac aaacgatgtc tattttagaa gaagaccacc aatcacccga tgattacgtg
     8461 atgatcgcaa ttggcggaac tggtgcagac ttctttaaag cacgcggtat caatctagca
     8521 tatgagctgc gcaatctatc tgatcagcca agctttgatg aagtccgcaa aatcgtaaat
     8581 atggcaacaa ctatgtacca aaacgaagta ttcgatgaat tatacgtatg ttataaccat
     8641 catatcaatt cgctaactag tcagtttcgt gtagaaaaaa tgctgccaat ctcagactta
     8701 gatccagaag aagcaactac tttcgaacaa gaatatattt ttgaaccttc aaaagaagaa
     8761 atcttgtctc agttattgcc tcagtatgct gaaagtttga tctacggtgc gatcgttgac
     8821 gccaaaacag cagaacatgc tgctggtatg acagcaatga aaacagcaac tgataatgct
     8881 gctaatatca ttgatgagct gacggtttct tataaccgtg caagacaagg ggctattacc
     8941 caagaaatta ccgaaattgt tgccggagct tcggcactag aatagtattg agattggagg
     9001 aaaaaagatg agttcaggca agattgttca agtaatcggt cccgttgtcg acgtggaatt
     9061 ttctttagac caatccttac cagatatcaa caacgcatta gttgtttata aaaatgacga
     9121 aaataaatca aaagttgttc ttgaagcagc gttagaatta ggggacggag tgatccggac
     9181 cattgcgatg gaatcaacag atggtttaca aagaggaatg gaagtcattg atacaggcaa
     9241 agcaatctct gttcctgtag gtaaagaaac attaggtcgc gtattcaacg tactaggaga
     9301 tacgatcgat ttagaaacac ctttcccaga agatgcggaa agaagcgaaa ttcataaaaa
     9361 agcaccagcc tttgacgaat taagtacaag tacagaaatt ttggaaacag ggatcaaagt
     9421 tatcgatttg cttgccccat atttaaaagg tgggaaagtc ggactattcg gtggtgccgg
     9481 tgttggtaaa accgtactga tccaagaact gatccataat atcgcccaag aacatggtgg
     9541 tatttctgta tttactggtg taggtgaacg tactcgtgaa ggtaatgact tgtattatga
     9601 aatgaaagat tcaggagtta tcgaaaaaac agccatggtg ttcggacaaa tgaacgaacc
     9661 accaggtgca cgtatgcgtg ttgctttgac tggtttgacg attgcggaat atttccgtga
     9721 tgtagaaggt caagatgtac tgttgtttat cgacaacatt ttccgtttca ctcaagctgg
     9781 atctgaagta tcagccttgt taggacggat gccttctgcg gttggttatc aaccaacatt
     9841 ggcaacagaa atgggtcaat tgcaagaacg tatcacatct acgaaaaaag gttcgatcac
     9901 atcgatccaa gcaatctatg tgccagccga tgactacact gacccggcgc cggcaacagc
     9961 attcgcccat ttggatgcga caaccaactt ggaacgtaaa ttaacagaac aagggattta
    10021 tccagcggta gatccattgg cttcttcttc aagtgcttta gcacctgaaa tcgtgggaga
    10081 agaacattat aaagtagcaa cagaagtcca acacgtattg caacgatatc gtgaattaca
    10141 agatatcatt gctatcttag gtatggatga attatccgat caagaaaaag tgttagtttc
    10201 tcgtgcccgc agagtccaat tcttcttgtc acaaaacttt aacgtggctg aacaatttac
    10261 tggtcttcca ggttcatatg tccctgtaga agaaacagtg aaaggattcc gcgaaatctt
    10321 ggaaggcaaa tacgatgatc ttcctgaaga agctttccga agtgttggcc gaatcgaaga
    10381 cgtcgtagaa aaagcgaaaa cactaggcta ctagaaaggg gtgccccatg gatcaatatt
    10441 taacagtaaa tgtggtaact cctgccggtt tggtctatga tcaccatgca gctatcgtcg
    10501 ttgcacgtac gacagctggt gagcttggga ttttgcctaa acatgcacca atcatcgttc
    10561 cattgacgat tgatgaagtt cgtgtaaaac gaacagattc agatacccac gtcgattgga
    10621 tcgctgttaa tggcggaatc atggaagttc gtgataatgt tgtttcaatc gttgctgata
    10681 gtgcggaacg tgaacgtgat atcgatgtat ctcgtgcgga acgtgcaaaa caacgcgcag
    10741 aacgccaaat cgaagaagca aaagcaaaac aagatacgaa cgaattgaaa cgggcaactg
    10801 ttgcgttgca ccgagcaatc aatcgtatca aagtatctaa gcactcataa aatgagatgc
    10861 ggatgtttat ccgtgtttaa gaacagtaaa aagaggttat gacagaagtg gtcagcttca
    10921 agacataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatc tcagcttatt
    10981 tttcgaagaa gctacttctg gaacaccgta tatcagaggt tgtaaaatca gcgttttgtc
    11041 ctgagtatta gagaaacaaa tggaaaatag ttcctcatat tttgccgttt tttgagctaa
    11101 taccgagggt cagatgattg aacaccgttt attt
//
LOCUS       DAPBAB010000073        10758 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPBAB010000073 DAPBAB010000000
VERSION     DAPBAB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.078"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(58..1041)
                     /gene="istA"
                     /locus_tag="RD444_002356"
                     /pseudo
     CDS             complement(58..1041)
                     /gene="istA"
                     /locus_tag="RD444_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1436..2278)
                     /locus_tag="RD444_002357"
     CDS             complement(1436..2278)
                     /locus_tag="RD444_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7549708.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2603..3019
                     /locus_tag="RD444_002358"
     CDS             2603..3019
                     /locus_tag="RD444_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7549709.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3016..3486
                     /locus_tag="RD444_002359"
     CDS             3016..3486
                     /locus_tag="RD444_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549710.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3500..4273
                     /locus_tag="RD444_002360"
     CDS             3500..4273
                     /locus_tag="RD444_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549711.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4287..5099
                     /locus_tag="RD444_002361"
     CDS             4287..5099
                     /locus_tag="RD444_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7549712.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5153..6118
                     /locus_tag="RD444_002362"
     CDS             5153..6118
                     /locus_tag="RD444_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7549713.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6234..8957
                     /locus_tag="RD444_002363"
     CDS             6234..8957
                     /locus_tag="RD444_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7549714.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8970..9602
                     /locus_tag="RD444_002364"
     CDS             8970..9602
                     /locus_tag="RD444_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7549715.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9807..10034
                     /locus_tag="RD444_002365"
     CDS             9807..10034
                     /locus_tag="RD444_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDT7549716.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10103..10369
                     /locus_tag="RD444_002366"
     CDS             10103..10369
                     /locus_tag="RD444_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7549717.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10359..10628
                     /locus_tag="RD444_002367"
     CDS             10359..10628
                     /locus_tag="RD444_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7549718.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3563 a         1715 c         2104 g         3376 t
ORIGIN      
        1 ataaatagtt tttctatcaa gtatattgaa ctacgataaa tttgttattt tgttttgttt
       61 aatttcgttg tacttattgg ctttgacttc gatttcttta aatactgggt aatcggtgtc
      121 cggcaaagct agcaaatctg ttttttctgt gttccataga tcattgataa tgacatgttt
      181 ttcgtaatgg attcgctctc gatcgtgtga cagctccacc gctaatgcct cgttgaaact
      241 ggcgaaatcc ttcataatcg gtgtagatga aaagaagttg taccgtacat agccgacttt
      301 gttttccata ctacctttct cgtttccgct acgtggattg cacacctgtg tctcaaaacc
      361 atagtgcata gaaaatcgga ggaaaccgtc agtcagcacc gcttgttcag tctttgtttt
      421 tgtctaagta acggccgttg acatattatc aatccgtatt ttccgaggaa cgccacctga
      481 ttggctgaag agttctttca tgccttccaa caaacattct tgattctcgg cggggagtac
      541 aacggcgaat cctgcattac taaaagggaa agtcatcact aataccttca catctttaaa
      601 cgctccatcg tgacaaactt ccatcgtacc aaaatcaagt tgcgcttctg ctggcgggtg
      661 ttcaagacgc tcaaagcctt ggtcttccag tgtttccaag tagtgtgtcg ctttccaatc
      721 ttgaatgaat acacagactg ttcggtagga tcctggaaag ccatcggctt ttaaagcagt
      781 agtcattgct tcagcagtcc tacgcttttt atttggcagc ttagcatctt ctgaaagcca
      841 tagggaaacg atgcctcccc acttttctgt atacatcata ccagtctttt tgcgtgcttt
      901 aggttgcggc agcaaatcat catctgcata ttttttagcc gtgcgccaat ttacatctag
      961 ctcctttgca atatgattaa tggatttatc atgattattt cgtaaatttc tgatagtatt
     1021 aatatcggtc attgtgagca tacctttctt acctccagtt acagtgtcga gactataact
     1081 ttagaggagt ttcaggttgc ccgcaatggc tttttatctt aacttaatcg atttgcggag
     1141 ttgtgtagtt tttcgttgca cttgtatgta cttttattct gccataaaca gcttaatgaa
     1201 tgtccacttg aaaaagtgaa taaacgaaat agatgactcg atttaataag gaagtaaacc
     1261 cacacagaaa tataggtgat tttccttgaa tgaccaagat attgaaagtt agtgaaaaaa
     1321 cacaaaaagg tagccctatt cacagaaggt ctgccttttg tgttatcgcc agcaaacatt
     1381 agagggggta tttcatatag cttttgtcct ctcgacagtc ctatcagttt ctcttttaac
     1441 ttatctctaa gtgctgtcca gttttaatag cttctagctt gtttcctaac tgtgttttta
     1501 atatgttgaa ggcttttgaa cctgtgcagt ggcaagtaag gattttttct actcgtgagt
     1561 cattcaattt atttccaatt ttcattacct cttgctcagg tagacctaaa ttatttatat
     1621 aaggtatgcc aatcatatgg aagccaccaa ttaggtattt tatgggttta gtcggaaatg
     1681 tttcaagagc aatttccatc atatcgataa tgccatgatg actacagcca gtaaagacaa
     1741 tcagaccgtc ttcttgctca attacgacca ataattcatc tgaaaaatca tcataaacaa
     1801 atttatcatc catctttttg tataaattct tggtggtcga gtttgctttg cctaggggga
     1861 tgatatggaa gctcggagca agttctgttt tcgtttttat tgtatgtaaa ttctcatgca
     1921 ttttaaggat ttcattttta ggaataccaa catacagaaa cgtggttaaa atctttaaat
     1981 aatggggtaa agttacacct tcagctgtat aaataggggc gtttcctgat tttaaatatc
     2041 ttaacccgcc ggtgtggtca gaatgaccgt gtgaaataat cactgcctcc agatcattca
     2101 aagtttcccc cattttagtc gcattataga ctaatttttt attagcgcca gcatcgaaga
     2161 gatatttctt ccctgagtag ttaattaaga gagataaccc atgtccactt ttaaagttca
     2221 aatttattct tgagttttca aataaaacct tgatttccac tataacatct cctttcacat
     2281 gaacgtgtat tcatatattg tagcacatta agttctattt tttgttgtaa ttaccgaaaa
     2341 aaatattaat ggacgctttt tacgctttat ttcgtatgtg gaagaaaatt tcttaggatg
     2401 tggcggggga ttggggcaaa gctaaattcc aattacagtg atcatttaat tagacacctt
     2461 taaataccca cactttcatc aacactataa tttagggaaa gaaaagtgtt ggcatgatat
     2521 agaaaaacct ttaattaaca gcgtttccta aaattggcac gcatcttgct tatagaaaag
     2581 cgtaaggata ggaggaatga aaatggcaac tataattctt gccagccatg gggaatttgc
     2641 tcaaggatta aagcaaacag cgacaatgat tattggagat agtgtaccaa ttcatgcctt
     2701 gtcagcattt agagatgaag atgaatctat tttggtacaa ataaaaaaac ttttagccac
     2761 aatagatata gaagaaacct acattttaac tgatattcta ggtggtagtg tgaataatga
     2821 tatgctcact attattaaag agtatgaggg attgcaatta attactggaa tgaacttacc
     2881 tttagttatt tctattgcaa cctataatgg aaaaattggg gaaagtgagc tagaaaagct
     2941 tattaaagag agtcaacaat cagtgattga ttgtagaaaa ttattagtag aacaacttac
     3001 tggaggagat gacctatgat taagttagta cgtattgacc atcgtttgtt gcacggacag
     3061 gtagtatttt cttggagtaa atcattaaat atcaatcgta ttattattgc caataaagag
     3121 gcagcgaatg atgaattcaa aaaaatgtct ttgaatttat ctaaaccgac tggcattaaa
     3181 ttaacaattt tttcagttga acagttacta gaaaaaatgc ctaaaatcga aacacttaac
     3241 gaaaatatca tgcttatttt tggtaataca aaagaaattc gtcaattttg tgaagcctat
     3301 cctaaagtac aagaaatcaa ctatggaggg ataatcaaaa aagaaggttc caagcaattt
     3361 agtaatgcca ttttcttgaa tgaatctgag caagaagatg ccaaaaaatt aaaagctctt
     3421 ggcattaaac aatttattca acaggtacca acttctaaaa aagaagattt gaatacaatg
     3481 ttataaagga ggaatgaaaa tgctcgttca gtcaattggt ttagggttaa tcggtgtttt
     3541 ttgtatttta gactcaaggc ttttaggaag aatgaatttt gaacgaccgt tgattgtcag
     3601 tacgcttgtt ggtttggtac ttggagacat ggaaaaagga ttaattgttg gagcctcttt
     3661 ggaattgatg tccttaggat tagtaaatat cggagctgct tcaccacctg atatgaatat
     3721 ggcatcaatt attgctacag cctttgccat tttatcaaat gcgaatgcag aagcagcatt
     3781 aacgattgcc attccaattt cagtgttagg tcaaatacta ggtatcttaa tgcggacgat
     3841 tttgtcaaat ttgactcacg ttgcggatcg ctttatcgaa caaggaaaat ttagccaagc
     3901 aaggagaatt catatacttt atggctcggg attatatgct ctcatgtatt ttattcctat
     3961 ttttctagca atttattttg gaacgaatct tgtttctcag attgttgatg cgattcctga
     4021 gtggctaaca aatggcttaa cggttgcaag taaaatttta ccagcatatg ggtttgcgtt
     4081 gttattacaa acgatgttaa cgaaaaaaat ggtgccattt ttattacttg gatttttaat
     4141 tactgcttat tctggattgg gaatcattgg tattgcagcc ttcgcatgta ttgttgcgtt
     4201 tgtcatggtt caagttcaag gtaaaaactc aaatggtaac caaaatgagc cagatccatt
     4261 agatgactta taaggaggta gattaagtga aagagaaaga tcaaaatgca tggaaatatc
     4321 ggcagttctt atggcgttca tgggcaattc aagcctcatg gaactatgaa cgacaaatga
     4381 acatgggttt tatgtacgga attgcaccaa ttctagataa catttacaag aatccagaag
     4441 atgaagcaat gaagaaagaa gcttacgagc ggcatatgat gtactacaac tgtacccctc
     4501 aaacaagtgc atttgtgtta gggttaactg cttctatgga ggagcaatac tacgaagacc
     4561 aagaaaactt taatccggaa tcaatcagtg cagtgaagac ttctctgatg ggaccgctat
     4621 ctggtgttgg tgattctttc tttcaaggaa cgattcgggt attagccttt ggactaggaa
     4681 ttaatttagc ccaacaaggt agtattttag gtccaattct agcaatgtta atttcctttg
     4741 taccttcctt tttagttact tactatggtg ggaaaattgg ctataacatg gggaataaat
     4801 acttgtctaa actgtatcaa gaaggtttga tggataaagt aatgtatgtt tgctccattg
     4861 tggggttgat ggtcattggt tcgatgattg ccagcatgat cgggattaca acaccaattc
     4921 aaatgggtaa agcttttgtc ttacaagatg tattagatag tattatgcca caatttttac
     4981 cattaggatt aaccttcttc atgtactggt tattgcagaa aaaggtcaaa acaggatgga
     5041 tgttggcaat ctgtttagtc ggaggaatta tttttagttt gttaggtatc ttacaataag
     5101 ggaataacca ataaatttgg ttgaataata taaataagga ggaattcaat tgatgaattc
     5161 aaaagaaatt gttaaagaaa tcgtagcgaa aaaagaggta aaatcaatcg tatttgttgg
     5221 ctgtggcgct tcaaaagcgg acctttatcc tgcaaagtat tttctagacc aaaatgcgaa
     5281 gcagttacgg attagtcatt acacagccag cgaatttaac tatgcaacac cagcatctgt
     5341 agatgagtca actgtcgtta tttcagcttc tttaggaggt acgactccag aaacagttga
     5401 agctaacgca gtagctaaag aaaaaggtgc tacagttatt agtctaacac gaagcattga
     5461 ctcaccgtta acaaaagatg cagattatgt aatttatcat cgatttgcag agaactatgc
     5521 tgctaaatta gaaaaaacag gctactgttt agaattggca gtagaacttt tacatcaagt
     5581 agaaggttat gaccattatg aagcaatgca agaaggattt gctactatct atgatcttgc
     5641 ccaaaaaagt gcagaatcag ctcgtaaaga tgcgaagaaa tttgctgaac gctttaaaga
     5701 tgaaaaagtt atttatgtaa tgagtagtgg tgctacacat gaagtagcat actcaacatc
     5761 aatttgcttg atgatggaaa tgcaatggat taattctggt accttccatt caggagaatt
     5821 tttccacgga ccattcgaaa ttgtggataa agacgtaccg tttatattat taatgaatga
     5881 tggaaaaact agaaatatag atgctcgagc attaactttc cttgaacgct ttgatacgaa
     5941 aacagaagtt gtagatgcct tggattatgg cttgtcttca catattgcta aagaagtctt
     6001 agactacttt aacccattcg tgattactgc agtatttaga gtttatgcag aagaattgtc
     6061 agaagcaaga gaacatccat taactaagag acgttatatg tggaaactaa cttattaaaa
     6121 aatagatcaa actaaataag gtggcttgag aaattaagcc accttgtttt cttcaatgtg
     6181 attttataca tatggtaaaa taactagtac atattgtgaa agcaggtggc aagatgcagc
     6241 gattaaatga ctatctttat caactaacat tgcttttgaa atctacgcct tcagataatt
     6301 attcagctcg cagtatttcg caagctctac agttgaatag aagtacaatc agtagctact
     6361 taaatgaagg tgttcgtgaa gggcttttca taaaagtaaa aagttacccg gttttatttt
     6421 tacatagaac agctcttgaa gaactacata tcacactgaa taactccgaa atagaatcaa
     6481 ttgaaagctt gttaacaatt tctcaaaaac ccgcattaga tcaagtaatt ggctctaaag
     6541 gaagtttgaa agaagcaatt gatcaaatta aaacggcagt cttatatcct ggaaaagggt
     6601 tgcctttatt attgatcggc gctagtggat cagggaaaac ctttctagcg aataaaatat
     6661 atgaatatgc agtagaagaa aaggtcattc acacatcggc acctttcata gattataatt
     6721 gtgcacaata tgtgagcaat ccagagttac tatcctctgc tttatttggc tacacgaaag
     6781 gagcatttac cggagcaagc caggagcata ctggtttgct agaaaaagcc gatggaggag
     6841 tattgttttt agatgaagtg catcgcttat ctgaagaagg gcaagagaaa ttatttacat
     6901 ttatggatac gggtgaattc tctcctatgg gggataatag tattcggaaa aaagccgatg
     6961 ttcgattagt ctttgcaaca acagaaaata tatatacgac tttcttacca acttttctta
     7021 ggagattgcc agttattgtt aatctaccta gatttcaaca acgtccgtcg tttgaacgtc
     7081 tgtcattgat tgatgaattt tttgtttctg agagccaaat tttagctaag gaactatctg
     7141 tttctgatgc attgattcat ttcttaatga atagtgattt tgaaggtaac gtggggaata
     7201 ttaaaaatat tatcaagtat gcttgcggaa acgcatatat ccaccagaag agtcagcaaa
     7261 caattcaagt taaattattg gatatgcctt tagagtacag ttataaattc aaggagcaac
     7321 taaataaacc cagaaagaaa agagcagatc gaatctacta tccagaagaa acacgacaag
     7381 tgcaactaga aagaaacaat cagctttttg ttcaattttt tgaccaatta cttactggat
     7441 tctctgaagt gattgaaaat aacaaggata ttaaactatt tttagaagaa atggttacca
     7501 aggtaacaca agtaatggat acgcttattt ttcaagagga ttacgaaaaa gagcagtctt
     7561 tatacgccat tctaaactat catattcgtc aaagtattga ttttatgcga gaaagctacg
     7621 ggtttgaaca agatggtaac cgggtagtcg ctttggccaa ctatctatat gtcaaggata
     7681 atcaagtgat ttttgaaaat agtcttcaag aaaagaaaac caaagatcag cttaacgaat
     7741 tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta agccttttat
     7801 ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg ctatattttt
     7861 atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta gctcatggtt
     7921 attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag aacgtttttc
     7981 aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa ttgattcaat
     8041 tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg ggttcactaa
     8101 accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc attgatcata
     8161 tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa agtatcaccg
     8221 aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta cttcccaaga
     8281 aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt gcgacccaga
     8341 ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta attattccgt
     8401 atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa ttatacgatg
     8461 tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat attggattag
     8521 atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa aaagagctga
     8581 ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt agtaaaattg
     8641 tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa aaagcaatta
     8701 aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt ttgttgtacc
     8761 ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat gagcaagagg
     8821 acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat agcgcttttt
     8881 ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga ttattgagcg
     8941 atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt gatatggatg
     9001 gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc tttcaagaac
     9061 tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca gaagctgcaa
     9121 tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat ccgaaatatg
     9181 tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa gaagtgaaac
     9241 cgttacttga cttcctcaag aagcgggata taccattggc actggcatct tcttctccag
     9301 ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt tcctttgtta
     9361 taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta aaagcgaaac
     9421 agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt attaaatcag
     9481 caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc gatcagagtg
     9541 aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta gaaaaaggtt
     9601 agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta accaaaaaaa
     9661 cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa aatgcttcgg
     9721 cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc taaggtcatg
     9781 tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg aatagttatc
     9841 aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact gctgccgtaa
     9901 ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat tggagaaaat
     9961 cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat accgtgctat
    10021 ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg ttgtattgac
    10081 aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta gaatcatgtc
    10141 tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat tagaatatgc
    10201 aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg ataaaatcga
    10261 agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag agaaaaaaga
    10321 gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa gtcatctaca
    10381 tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa ataattgtcg
    10441 cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga aaaggtttga
    10501 ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata ttggctaata
    10561 tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt acaatatata
    10621 aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa tcttttgaat
    10681 tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct gcttttttat
    10741 ttggtcttga agaagaaa
//
LOCUS       DAPBAB010000074        10646 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPBAB010000074 DAPBAB010000000
VERSION     DAPBAB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.079"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            23..646
                     /locus_tag="RD444_002368"
     CDS             23..646
                     /locus_tag="RD444_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549719.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="RD444_002369"
     CDS             1096..2145
                     /locus_tag="RD444_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7549720.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="RD444_002370"
     CDS             2142..3023
                     /locus_tag="RD444_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549721.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="RD444_002371"
     CDS             3026..3796
                     /locus_tag="RD444_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7549722.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="RD444_002372"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="RD444_002372"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDT7549723.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="RD444_002373"
     CDS             5107..6270
                     /locus_tag="RD444_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7549724.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="RD444_002374"
     CDS             6387..6797
                     /locus_tag="RD444_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7549725.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="RD444_002375"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="RD444_002375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7549726.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="RD444_002376"
     CDS             9370..9654
                     /locus_tag="RD444_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549727.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="RD444_002377"
     CDS             9814..10050
                     /locus_tag="RD444_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7549728.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="RD444_002378"
     CDS             10047..10445
                     /locus_tag="RD444_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDT7549729.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3715 a         1773 c         2253 g         2905 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttat
//
LOCUS       DAPBAB010000075        10616 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPBAB010000075 DAPBAB010000000
VERSION     DAPBAB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.080"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            288..2234
                     /locus_tag="RD444_002379"
     CDS             288..2234
                     /locus_tag="RD444_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549730.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2237..2692
                     /locus_tag="RD444_002380"
     CDS             2237..2692
                     /locus_tag="RD444_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549731.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2706..4034
                     /locus_tag="RD444_002381"
     CDS             2706..4034
                     /locus_tag="RD444_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDT7549732.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4068..4352
                     /locus_tag="RD444_002382"
     CDS             4068..4352
                     /locus_tag="RD444_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549733.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4354..4869
                     /locus_tag="RD444_002383"
     CDS             4354..4869
                     /locus_tag="RD444_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDT7549734.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4885..5547
                     /gene="rpe"
                     /locus_tag="RD444_002384"
     CDS             4885..5547
                     /gene="rpe"
                     /locus_tag="RD444_002384"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDT7549735.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5554..6126
                     /locus_tag="RD444_002385"
     CDS             5554..6126
                     /locus_tag="RD444_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7549736.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6313..7833)
                     /locus_tag="RD444_002386"
     CDS             complement(6313..7833)
                     /locus_tag="RD444_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDT7549737.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8076..8261)
                     /locus_tag="RD444_002387"
     CDS             complement(8076..8261)
                     /locus_tag="RD444_002387"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDT7549738.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8245..8427)
                     /locus_tag="RD444_002388"
     CDS             complement(8245..8427)
                     /locus_tag="RD444_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549739.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8668..8886
                     /locus_tag="RD444_002389"
                     /pseudo
     CDS             8668..8886
                     /locus_tag="RD444_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9381..9980
                     /locus_tag="RD444_002390"
     CDS             9381..9980
                     /locus_tag="RD444_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7549740.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10044..>10616
                     /locus_tag="RD444_002391"
     CDS             10044..>10616
                     /locus_tag="RD444_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HDT7549741.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3509 a         1670 c         1983 g         3454 t
ORIGIN      
        1 tataataaaa aaatacgaat tttagtgacg ccattatatt gtttttattt ttcaattgta
       61 atatttcata ttacttccat aataaggagt attttctatt ggaatatctt agctctttta
      121 aggatttctc cttttttgtt tctcattttt taagattatt tggatttcct ttacaacaaa
      181 cttccacaag tattattgac atagatgtaa ttgagggaag ggggttccag tgctagaata
      241 aataatgaaa agaggtaaca aatgctagcg ttaagaggtg aacaagtttg attgaaagac
      301 atgtaaattt attaaaatta ttaagtcgac aagaagactt taaacccgct tcttttttta
      361 gtgataaatt ggcagtgtcg accaagacca tttatcaaga tattgaattg ttggaagatt
      421 atgtagttaa ttaccaagta acaattgagc gattaccttc tttgggtatc aaactcatag
      481 gtgataggaa aaacgttgaa catttgattt catcggtaga aaattatctt gaaaaagata
      541 tttattctcc agaaaaaaga cgtgtcgaga tcattaaaga agttttgttt ggtgataagc
      601 ctgtttcttt aactcatttg tctcaaagtt ttatggttag taaaacatcg ttatacaatg
      661 acctgagaat tatcaataaa attattggca gtggagaagt taagttgcag tcatctgcag
      721 aaggaattag ttttactggt aatgaacaag caattcaacg agcaatcaag caacttgtat
      781 tctattatgc gaatgacatg gaatcaaaaa cattttttca agttttaaat atgttgtttg
      841 attcaaaagt tattagtgtc attcatgatt tgctattcaa tgattacagt gaactgactg
      901 aaaaggtttc tgattactat gttcggtcat tgatttctac tttagttatc caatgtaacc
      961 gtttactgaa aggtcatcat ttaactgttg aagaagaatt cctcttcaat agcattcgct
     1021 atatggaaac gtacattgtg gcaaatggtt taattgaaga attgaaggca aaattgaatc
     1081 ttccatttta tgacgcagat aaagaatatc tatgtcgtca actatttgca catcgtgtga
     1141 caaataattt aaaaaccagc aatgaagagt acgctatgag cgttcataaa ttgattgaaa
     1201 gaatgagtga gattgaaaaa attgatttga ctcaaaatga tcgattatat caatccttat
     1261 tgtatcatgt gccagcaatg gtccttcgtt taaaaaaagg tattaaaatt caaaatccgt
     1321 tattagaaaa tatcaaatct caatattcag aactctttag catagtatgg tacgccatgt
     1381 ccatccttga agaagaattt gatgtgactt taacggacga tgaagtttcc ttaattttga
     1441 ttcattttca gattgctatt gaagatcagt ccaaagcaaa taatatcatc attatttgcc
     1501 aatacggtat ttcttctgcg caattaattt acaataaagt tcgacggttt ttaccagcaa
     1561 aagataatgt agaaatatca actttagata agtttcattt agctgaaatt ggggaagttg
     1621 atttgattat ttcctctctt tcctttgatg caggtggtat acccttagta agggtatctc
     1681 cattggtaag taaaacagat tatatgaata ttatggaagc ttatactaaa tatttaatag
     1741 aagaaccttc gcgatttatt caaaatgaac tgattcctaa agcggttaaa gttcccacca
     1801 tatctaaatt tgttcaatca tctttagtaa aaacaggagt acaggtggaa agcaaagaag
     1861 aatgtttgga tttgatgatt tccgaactag aagccaattc attagttact gaagagtttc
     1921 gtgcttctat cttctctaga gagaaaatgg ggaatactaa tttagaaagt ggtgttgctt
     1981 taccgcatgc gaatccatca atggtcattg aatccaacat ttttattgta gcattaaaaa
     2041 atgcgattaa ttggggcgat cgaaaagtca aattagtcat tatggttagt ttagctgaag
     2101 aagatgtatc tgagattaga ggagtatatg aagaacttta ccaatttatt gggcaaaaag
     2161 aaatggtcga tcgcttgtta caagttcatg atagcgaaaa attactaaga atttttcaac
     2221 aaaaaggaga ataaaaatgt atctagataa agaattatgt ttatttcaat tagaagtgaa
     2281 agatcaaaaa gaattgtttc aagtaatgtc tgaacaatta aaaaacgctg gttgcgtgaa
     2341 agatagtttc ttagaaggta tcaccaaccg tgaacaagaa tttccaactg gattagaagt
     2401 aaatcaaatt gggtttgcta tcccacatac agattccgca catgtaaata gctctcaaat
     2461 ctgttttgcc tcattaaagg aaccactggt attttcagat atgacagata aatctcatga
     2521 aatacctgta cgtttagtct ttatgttggc aatgtcacaa cctcatgaac aaattgatac
     2581 actacaaaac ttagttagcc tatttcaaaa tgaagaaaaa gtaaatgaat tacttgcatg
     2641 taccacgaaa gaagcattta ttgagttagt acattctgca ggagtttatt aggaggaata
     2701 gagagatgga tattgtatta aatgcctttg aatggtttat tggattaggg tcaaacgtct
     2761 ttttaccact gattattttt gtaatcggga tattatttgg attaaaacct ggtaaagcat
     2821 ttgtttccgg cgttactgtt ggtatcggtt ctattggttt aggattagtt ttaagtttat
     2881 tatcagatgg tttaggaaca gcaattcaac aaatggggga acaatacggt ggtggcctga
     2941 atatcctaga tattggcgta ggtgttggtg gacctttggc ttactctaca tcattgggaa
     3001 ttttaatgat tccaatttca ttaattctta acttcatatt tgtggttttg ggttggacaa
     3061 aaacattgaa tgttgatatt tggaactttt ggttcgcaat tttcctaggt atgattactc
     3121 aatctgttac gggaaacttt tggtttggct taattggcgc gattgtcgca gtaattttac
     3181 aatggttctt agcagatcgt acacaaaaaa ctgttagcga atttttcggc tatccaggaa
     3241 tctctatcac acatatgatg ggattatcag gagtactatt tgccaaacca gtaaattggg
     3301 tttttgatcg tattcctgga tttaataaaa tagatgcaga tgcagaaaca ttaacaaaac
     3361 gtttcggtgt tttaggtgac acagttgtta ttggcctaat tatcggtatt gtagtgggta
     3421 ttatggcagg ctatgacgta gcaggaattg gaaacttagg aattatgact gcggcagtga
     3481 tgaagattat gcctaaaatg gtagcaatgt ttatggaagg tttaatgcca attgcagaag
     3541 cggcgaaaga atttacgaat aaaaagttaa atggtcgcca agtaaacatt ggaatggatg
     3601 cagcgttaac tgtgggtcat ccaactatta tgtcaacttc tctattgctt gtaccaatct
     3661 cattattgct agcactaatt cttccaggaa ataaagtatt accttttgga gatttagcat
     3721 tctttgcttt cgccatctgt ttaatgattc cttattttaa aggaaatatt gtgagatcat
     3781 tagtaggatg ttcgttgtat gttgttacaa cattatatat ggcaacatgg ttggcaccaa
     3841 ttatcactga tgtgtttaca ttagcaagtt tcgatgtggg gacagcagga ttagtaacta
     3901 tgttgttatg tggattatgg ccagtagggt tgtttgtttt attagcatct tactttggtg
     3961 taattggaat aggtggatta ggagttgttt tagtaggtgc ttctatctac atgaacaaag
     4021 tgaagaaagc ttaatcataa aattaaaata agaaagaaga gatcaatatg aaaaaattat
     4081 tagttatttg tggagcaggt catgctactt ctacaattgc agtagcaaaa ttatcaaaat
     4141 ggttggaaga agaaggatta acagatcaag tgaaaattta tcaatctaaa attgctgatg
     4201 agctaaataa aattgatgaa tatgatgctg tagtaagtac aacaattgtc cctgatgtaa
     4261 ttaaagctaa agtaacgaat ggcttgggct tattaacagg tattggtaca gatgccattt
     4321 atgcagatgt aaaagaaaga ttaggtgttt aatatgcgtt ttcatcatga cactttgact
     4381 attgaaacag tttcagggcg tccttcgtat catgatattc gaaaagaact tttagataca
     4441 gttgatacag caggtattga gaatggaatg ttagtaattt cgagcccgca tacaacttgc
     4501 tcactctttt ttgaagaaac gatgcatgac acaaactttt ttggtgatga ttatttacat
     4561 gtagacatca acaatgtaat ggaaacgatt attccaaaaa tgacgagtga aaatcaatac
     4621 aacagtccgg ggccaaaaca cattgatttt ggaacaagat tatctgatcc caattaccct
     4681 gcagaaaaat gggtcatgct aaataccgat gcacatttac gttcatctat ttatggaacc
     4741 aactcaatga ctttaattgt tcaagaaggt aaacttttat taggagaaat gggccgtgtc
     4801 tattttgtcg attgggatca actaagagag cgaactagaa caatcaatct catgattatg
     4861 ggagaataaa cgggggaata gagaatgaca attgctccat cattgttaaa tgcacctatc
     4921 gcagaagtta aagagttatt tgaaatctta aaagaaaaga atgttgatta tttgcatatt
     4981 gatgtaatgg atggctgttt tgtaccagat atggcttttg gaccaagtgt tattaaagat
     5041 attcgtccac taacagacgt tgtgtttgat gttcatttga tgattgatac accagaaaaa
     5101 attgtcacaa gatttgtaga agcaggagca gatattgtta ccttccacat tgaagctacc
     5161 aaacacgcaa tgaaggtcat tcagttagtc aaggaagcag gagcaaaagt agggattact
     5221 tttaatcctg caacaccagt cagttttatt gaaccagtat tatctttagt cgatcaagta
     5281 ttagtaatga caatcaatcc aggaacagag aataagcatt ttattcaaga gacagtagtt
     5341 aaaatcgaac agttagatgt aatacgcaaa caaaatgatt atacgtatga tattgaagta
     5401 gatggcaaga ttgataatca aacgattaaa gtttgttcta aagcaggggc ggatattttt
     5461 gtatcaggcg gtttcatctt caatgggaaa aataccccgc aagaacaaat tgataaacta
     5521 aatcaatcat tagaggaagt gaagtaaaag atcatgacca ataaagacca aaataaaaac
     5581 tatttagaag cgattatctt agaattacaa aataactcaa aacgtattaa ttctgtagag
     5641 ttagaacaat ttagtgagaa aattgttcaa gctaaacaca tctttctagc tggtgcagga
     5701 cgctctggaa ttgccatgca agcatttacg aatcgcttaa tgcatttagg tttttcagtc
     5761 agccttgtcg gtgaaattag ttcaccacat tcgcaacctg gagatttatt aattatctgt
     5821 tctggctcag gtgaaacagg tagtttaaaa aatttagcgg ccaaagcaaa tcaaagtggt
     5881 gtaagcattg ctttagttac catgaaaaaa gactcgtcga ttggtcaatt agcagattct
     5941 gttcttgtat taccaggtat taccaaagat gaaaatcaac gagaagaaga tactttttca
     6001 caacctatgg gttcaagttt tgaacaatta gcatttctca cctttgatag tttagtgtta
     6061 tctttaatgg ataaaacagg tgaaagctca gaaacgatgt ttgctcgaca tgcagatttt
     6121 gagtagtaga ttttaaagat aaaaaaccat tctctaaatg ctaattattc agcaaatatg
     6181 agaatggttt ttttatttga ttagacataa cttaataatt cagtagtttt gagcgtaagc
     6241 gtgtcataga atggtaccta aacacaaata gcccgatttg atacactcaa atcaggttat
     6301 ttgtggctta tattatttac agatttcttg tatctgctga ttccatggca aataagcgcg
     6361 tagcgcttca ggtgtcatcg aagacagatt gggaatctct gtaaaaagat gatttaaata
     6421 tttgcggatg tttaacccat tcgcaacagc tgtctggata atactcaata taattccatt
     6481 tgatctagcg ccttttaagc ttttagaaaa tagccagttt tttcgaccga tcactaactc
     6541 tttgatggca cgctctgctc ggttatttga aagttccaag cgtccatcaa gcaacacaat
     6601 ctgaagtccg tctttatgat tgactgcgta ttgaaccgcc tttccaagct gcgattttgg
     6661 caaataagca aaactctcac accaatgaaa aaattcagtc atcacaggct gcaacgtttc
     6721 cttcctcgct acaaaccttt cttctgcgct aagcttatta aaggtccgtt cgattcgaaa
     6781 catcttgtca cagaagtcta atcctatttt ggcatgactt tttggggacg gttgtttacc
     6841 caatgcttca aaaaactttc tgcgaacatg cgcccaacaa taaacattgt ttaccggcag
     6901 ctttccatag gccgcgtaac catcagtatg caagaaaccg ctaaatgatt ctaaaaaact
     6961 ttttggctca tcaacggaac gcgaatttgc gtgctcgtag agcacaactt gtttttcttc
     7021 gcttttacct gaagcatata accagtaaaa ggtcttttct ttatgagtat tcaatacccg
     7081 atagcttgtc tcatccgcgt gtaagacctc ctgattgctt aattcttgat gaaggcaatt
     7141 gtagattggc tctaaaacca tttcacttac cttgatatgc caattagtga tttttttacg
     7201 atcaagaggg aaaccaagtg catgccattc tttttcttgt cgatatgccg gcaccttttt
     7261 aacaaatttt tcccaaatac tctgtgtcac aattgaagag gaacctagac tattattgat
     7321 caacggttta ggaacaggcg cttttttgat ttttgtttct cctaatgctt cacattctct
     7381 gcaatgatag gcatgttggt agatgacttg tttttctata tgggcaggaa catacaatgg
     7441 ctcttctcta agccttgtcg caccgatatc atgtaaagta tgaccacagt cttcacacaa
     7501 acaatcggca ccttttaatt cataatgaat aggaacttca ggcagacctt ctaatgaaag
     7561 tgctttgagt cccttgcgtt ttctgcgttt aaaagaagat accgtaatgg gttcctggct
     7621 ttcttgtcca gtgtgctctg gttcattaaa agttgagtcg tctaataaat cgatttgccc
     7681 atctacgggt ggtgtttctt tttttcgtcc aaacaagcgt cgattcattt cagcaatttg
     7741 ttctctaaga agagtatttt cctgttgctg gatttcaagc aactgtgtta attgcttgat
     7801 ttgtacaaga agttcagatt ccatttttgt catttttaca ccaccattct agcgattttc
     7861 ttacatctat tataccattt tatgacaacg aaaaacagac aaaatccttg atttaacaag
     7921 gttttcgtct aatatatcgc accttttttc gtttctttta gcgtacattt ttcttcgatt
     7981 gaaaggccag tcagtaagac ttcttttctg gaacgcggcc attgaaggta cccactttca
     8041 aatcttttgt acattaatac aaatccattt tgatcctagt aaagagcctt gaagcgatct
     8101 ttcttcgtac cgcaaaataa gaaaagcgct tgatcgtaaa catcaaggtc atattcttct
     8161 ataacgaaag ctgctagtcc atcaattcct tttcgaagat ctgtttttcc acaaacgata
     8221 aaaatatgtt tcaatgcagt tgcatcaatg agcattggca ttcaactctt ttaacaaggt
     8281 atacaaaaga tatttatcca ttccattata aataaatatt tccttatctt ccgatttgat
     8341 tctgcaagct agttttggct tggtgtcaat ttgcgatttt tgagccgcac ttgaaaattg
     8401 aactggtaca atatcttttt tattcattcg tttttctcct tctatatttg atagtttaat
     8461 tatagaagaa agataatcgg cgtacgagat actatcctat gacacgctta catttaattt
     8521 aatattttgc ctcaaattag ccacactttt tatagaacta agaggaaaaa tgaagttttc
     8581 ttcctattaa tatagttgtt tgaaatctta aatatagcgt tttacgatgt aatgggaagt
     8641 atggtgaata aatcaactat gttcgtacga agcatttact caactaattt aatcgaatct
     8701 ttcaataaac aaatcaagaa atacagccac cgcaaggaac agttccaaaa tgaagagtcg
     8761 atggaacgtt tcttagtctc gtcttttgat acttacaacc aaaaattcct aggtcgcagt
     8821 cataaaggtt tccaacaggc cgaaggcgag ctcgaacaaa tgctgagtca accgatggag
     8881 aattagaaac aatctacagt agggaagaac cattgacaca aaattattga cgctcccaac
     8941 taaaagtgtc aaaataatga tgaaatcatt tagttcttat aaattagaaa agagaaaata
     9001 aatattccat atctacttta ttggatccgt aacatgacta aattctgaat actttttaaa
     9061 gcttatcatt tggtaaaact gaatataagt taattatata gactgaaaat tttgaatttt
     9121 cagaatagaa taacttaaaa gtactgacaa aattttttaa aggggcaata ttaatgttct
     9181 gagataatca aatcctaccg aaaatatcaa aaatccaatg tgacacaatc ggattgtgtc
     9241 tcattcgagg gaatcacaca atcccacgtg gctctaggca gaattgaatt tttgcttggg
     9301 gctttttcct tatattgatt ttgaatgaga cttattatat gttataatgc aattaagtat
     9361 cattggagag ggcgaagaaa atgaagacgg tagaacctat tcgagacaaa aaaaagatcg
     9421 atgcgatgaa ggcaatcttg gcttcgggaa aatatggcca acgaaatttg gttctttttt
     9481 cgattgggat caacaccgcc tatcgcattt ctgatttgcg tcaattgaag ctggccgacg
     9541 tgctggaaat ttcacgcggt cgcgtggtgg tcaaagatcg attagcgatg aaggaacaaa
     9601 aaacagctaa acataattcg gtctatattt ccaacaaatt gcgaaaaacg attttggagt
     9661 acgttcaaca ggaatttcct gatcaactcc ttcagcaaga ttttgacggt tatttgttcc
     9721 ctagtcgaaa agggacgaat gaaccgctca accgccaaag tctgtggcga atcattcatg
     9781 atgctggcac ggcaatcgga ttgaaagaca ttggcccaca ttccatgcga aaaacgttcg
     9841 gttactttct ctataaacag gggaccaaaa cagaaatcat tcagtccttg ttgaaccatt
     9901 cttctcagcg cgaaacattg cggtatattg gtattacaca agaagacaaa gatacagctg
     9961 taaaaagctt agatttataa tataaaagtt aagtttaaac atggatagtt ttaaattagt
    10021 agattgagtg ttgggaggaa agaatgagta cattatatat tataggaaac ggttttgatt
    10081 taagtcatgg tttaaaaact agatacactg atttttcaaa atgggttgaa aaaagtcata
    10141 gtgaaatatt cgataaaata gacacaatga taaatgaaaa taatgaggtg agaagtacag
    10201 ctgatgataa gataggttgg tttaattttg aaagtagttt gcatgagtta attcaaagtc
    10261 cagtctttga agattatgta gaagagtcta aggattatct cataggttat ggacatgaag
    10321 attggtcaga ttctgcccat catgattttc aaaatatggt ttatgattat gttgtttctc
    10381 tagaagaatt acaacaactt ttcaaggaat ggatacgcaa agaaatacag ccgacaatag
    10441 aacataaaat tccaaaatac caattttcgt ccgatgtact attctttaca ttcaattata
    10501 cagatgtttt ggaaattcta tacaagcaac cgaaagaaaa catatatcat attcatggca
    10561 gttatgacga tataataatc ggccactccg agaattttga caactttata ggagat
//
LOCUS       DAPBAB010000076        10576 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPBAB010000076 DAPBAB010000000
VERSION     DAPBAB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.081"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            79..1383
                     /locus_tag="RD444_002392"
     CDS             79..1383
                     /locus_tag="RD444_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDT7549742.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1475..1840)
                     /locus_tag="RD444_002393"
     CDS             complement(1475..1840)
                     /locus_tag="RD444_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDT7549743.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1837..2202)
                     /locus_tag="RD444_002394"
     CDS             complement(1837..2202)
                     /locus_tag="RD444_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDT7549744.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2394..2642)
                     /locus_tag="RD444_002395"
     CDS             complement(2394..2642)
                     /locus_tag="RD444_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549745.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2641..2769
                     /locus_tag="RD444_002396"
     CDS             2641..2769
                     /locus_tag="RD444_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDT7549746.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2826..4520
                     /locus_tag="RD444_002397"
     CDS             2826..4520
                     /locus_tag="RD444_002397"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDT7549747.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4524..4643
                     /locus_tag="RD444_002398"
                     /pseudo
     CDS             4524..4643
                     /locus_tag="RD444_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4620..4793
                     /locus_tag="RD444_002399"
     CDS             4620..4793
                     /locus_tag="RD444_002399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549748.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5171..6304
                     /locus_tag="RD444_002400"
     CDS             5171..6304
                     /locus_tag="RD444_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDT7549749.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLELVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6335..6655
                     /locus_tag="RD444_002401"
     CDS             6335..6655
                     /locus_tag="RD444_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDT7549750.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6684..7148)
                     /locus_tag="RD444_002402"
     CDS             complement(6684..7148)
                     /locus_tag="RD444_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549751.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7277..8526)
                     /locus_tag="RD444_002403"
                     /pseudo
     CDS             complement(7277..8526)
                     /locus_tag="RD444_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8555..8872)
                     /locus_tag="RD444_002404"
     CDS             complement(8555..8872)
                     /locus_tag="RD444_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7549752.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8898..9218)
                     /locus_tag="RD444_002405"
     CDS             complement(8898..9218)
                     /locus_tag="RD444_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549753.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9619..>10576)
                     /locus_tag="RD444_002406"
     CDS             complement(9619..>10576)
                     /locus_tag="RD444_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7549754.1"
                     /translation="DRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNY
                     HKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDP
                     MVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNE
                     ETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGR
                     EHEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFK
                     NRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT         3377 a         2101 c         1858 g         3240 t
ORIGIN      
        1 ttatgtgggc aattctatgt aaataaagaa gtcaatctag aaaagctctc caatctcctg
       61 catgtttgca aaaccacttt gctcaatgat atcaataaca taaaaaaaga atttgaagaa
      121 cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc agaacatata
      181 cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa aacttgtctg
      241 ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga attcatctct
      301 gtcagcaaag cttattcatt aaaaaaacaa gtattagctt attttaatga ctgcgggatc
      361 gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct gcttcttaat
      421 gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt ttttgaacgt
      481 gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga taaagaaaat
      541 aaagaaatct tattgattgg ttttgctatc agtttcctac gccagcaagt gtgtgctgtg
      601 acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa
      661 tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt cgcctttatt
      721 ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat tgcggaagac
      781 tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga actagttgct
      841 acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccattcga acgtgcgctg
      901 attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga aaaattttat
      961 cttctaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt ttccagctgg
     1021 tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc ttttgtgatc
     1081 gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat cgtcacaaat
     1141 tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac tttcgatttt
     1201 caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc gcagcagtcc
     1261 tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca atatgaaaat
     1321 attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt gcaaaacaaa
     1381 taagggactg tgacaaaaat catgtcacag cctcttattt gttgaagatg cccatttctg
     1441 taacaatctc ttgatacaca tcaggctcac aacctcactt attttggaca tctacgggtc
     1501 cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac gccgctagtt
     1561 ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct gaaacaagaa
     1621 tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg ttctcgctga
     1681 ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc ttgattgata
     1741 gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt acaagttttt
     1801 caaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc ctcttctttg
     1861 tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc gaatgataat
     1921 aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact cgatgatatc
     1981 agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt ttcttgtatc
     2041 tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat caaaaagttt
     2101 gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat ttaaaatagc
     2161 aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta cctactttct
     2221 tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc tatttaaaag
     2281 ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccct ttttctccct
     2341 ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc
     2401 gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa
     2461 atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt atttacagga
     2521 aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg
     2581 cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga ggcgtttttc
     2641 atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct tggcgctgca
     2701 tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga tgaattcgaa
     2761 tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac attggaggaa
     2821 tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat atggcagcgg
     2881 ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt tattcattgg
     2941 atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa tattggaata
     3001 aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc atgcttaatt
     3061 accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt actgagccag
     3121 ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc tttgacaaag
     3181 cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga ttcaataata
     3241 aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa gaaaaactag
     3301 acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa acaaacttct
     3361 ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa gaattgcgtc
     3421 gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg ggcgtcaatc
     3481 gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac ttgaaagaac
     3541 aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa gaaacaccga
     3601 tgcaagatga aatcactgtg actggtttga aaatgaccaa tacactgact caagaagaag
     3661 aacacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc attacagatt
     3721 cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat ggtgcagctt
     3781 ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct gacaaattct
     3841 ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa aaccatttgt
     3901 tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg aattcattta
     3961 tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct atcgtcgtgc
     4021 atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg ggttatttcc
     4081 tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg aatggaaaag
     4141 aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca gtgaatattc
     4201 gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc tatatgcctt
     4261 atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc attcctaaac
     4321 attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag atgatcttta
     4381 ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg ggtatcccta
     4441 tgtcacgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta gctcgtgccg
     4501 caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     4561 gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta ttaccgcaga
     4621 tgcaagcttt tgaacaccgt ttaccaagag gttgtgaaaa agctgccctg catcaagtaa
     4681 taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttggac ttattaccgc
     4741 agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaagtcgtt tagtttcgag
     4801 tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt gacttaatac
     4861 cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc tttttgaaaa
     4921 caaccatttt ttcaacgttt actctcttgc caatccaaca tcatctaaca gatacaacta
     4981 tttcaatgat taccattaca ctaaaaaatc ggaaactcac ctctccgaac acaaatacat
     5041 aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa ggtaagatct
     5101 cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga atctgaaagg
     5161 agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa tcagaaacgt
     5221 agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag caacagccat
     5281 ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa aagggtatgt
     5341 tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc aaacattgaa
     5401 aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc atttctgcct
     5461 gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg gcgggctttt
     5521 cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc taagagttcc
     5581 ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg aaaaagtttt
     5641 atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag gagtaaaaaa
     5701 aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc aaaaattcgc
     5761 tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc gtctagataa
     5821 agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac aagctggaac
     5881 agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg taggaatgga
     5941 cggattagag cttgtcaaag aaaaagcgaa aaatttagta agcttacaaa acggcacaat
     6001 ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc aagtaactgg
     6061 tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc gggatatcgc
     6121 tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg aaatgattgc
     6181 agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat cagaagcagc
     6241 acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg ttttgaaacc
     6301 ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac gaaataccca
     6361 tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct tccgcttcta
     6421 tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg ggattttcta
     6481 tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca cctattttta
     6541 tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa ggaacatttt
     6601 tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg catgagattt
     6661 gaaaaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag acgagcggtc
     6721 gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc ataaaacgtg
     6781 gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc ttttctgatt
     6841 tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac aaacagggca
     6901 caaagatttg gtgtcaaatc agggcggtta tggaagtcgt tttcaataac gcccgctcct
     6961 ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact ttccttatta
     7021 gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca ttttgatgaa
     7081 aaccacttcg cactttgttt tactaattct gggtgttctc ttaatttgat ccagttatat
     7141 gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat acaagtcttt
     7201 atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa cggaaaacaa tcacttgttc
     7261 tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg atcgtagcta
     7321 ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg gctagtacac
     7381 cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt acccatggag
     7441 gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca acactaacta
     7501 agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg ataggtaacc
     7561 caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag tttgttacaa
     7621 ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa cctaaagttg
     7681 cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg ataaacggtg
     7741 cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca ttgattgttt
     7801 gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga
     7861 taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat ggttcttgga
     7921 ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa attgcagcaa
     7981 ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa
     8041 ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg cttaaacgta
     8101 caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg tttgagctca
     8161 tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct gcggaaacac
     8221 cgtcttttcg gtagccgtta gctaaatgat aagcaagaaa gcaatcaaga cagaaagaat
     8281 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8341 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8401 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8461 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8521 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8581 caatgatctc ttttgctaaa tctgtaaaag ctatggatgt catcagatga tcttgactgt
     8641 ggactgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8701 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     8761 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     8821 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     8881 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     8941 ccattcatca tgccatagtc agccatattg attatatcta aaggaatgcc tttaggtgct
     9001 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9061 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9121 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9181 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9241 cttacacttg tatataagca agtagcgtgc caactttgaa agcgctttaa aaagttgata
     9301 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag tgcacactaa
     9361 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9421 caaaacttgt cacaactttt tttcgaataa acggtattca aaagcttgca tctgcggtaa
     9481 taagtccaac caagttaaaa acataagatg ttgtttttac tttgctattc ttattacttg
     9541 atgcaggaca gctttttcac aacctctttt atttcaaata accagtaaat acaatgaaca
     9601 tctacaaaaa acttctattt atttatagat ttcgtcaggc aaatccaatt ctctcttatc
     9661 aatcaaagtc tgataccacg aagcagattt tttcaatgtt ctgcttcgtt catcgttcaa
     9721 ttcgattcga actaatccat atcgattctt aaatgcattc attggtgaca cacagtcagt
     9781 gaatgcccat aacatatagc cttcgcaatt tgcgccttct tctgttgctt tgatcaaata
     9841 acgcaaatgc tgtccgataa attcgatacg gtaatcatct tgcacgattc ctttctcatc
     9901 catataccgt tcttcatgtt ctcttcccat accgttttct gttatcagcc aagggatatt
     9961 atgataattt tcttttagat acatggctat atcatacatg attttcggat agatttccca
    10021 tcccctggaa ttattcattt tcttcccagg taaatcaaag acttcataat atcgttctgg
    10081 atggaaaggt gtctcttcat tccattgata acgaggagcg cgaacgcgtt taggataata
    10141 ttgattgatt ccaaggaaat ctactgtatg atgctgaatc gtttctaaat cctctgttgc
    10201 aggatcgaaa taaatcgatt gtttttgaca caactccagg aactcttctg tgtagtaccc
    10261 ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa tcatacatat aagcagcttt
    10321 ttggtcttct ggagaattag atcgagcata gaccatttca ggatttaaaa tacagccaat
    10381 ccttccacca taatttccgc tgcggtaaat ctctactgct ttagccgttg ctaaaacttt
    10441 atgataattc cacatcatcc acttttttgt gttttgttca tgcggccagc gaacagcatc
    10501 caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat gtaaaccagt tttttaccct
    10561 atcaccgtaa cgatca
//
LOCUS       DAPBAB010000077        10533 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPBAB010000077 DAPBAB010000000
VERSION     DAPBAB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.082"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(1..656)
                     /locus_tag="RD444_002407"
                     /pseudo
     CDS             complement(1..656)
                     /locus_tag="RD444_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(661..1428)
                     /locus_tag="RD444_002408"
     CDS             complement(661..1428)
                     /locus_tag="RD444_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7549755.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1916..2341
                     /gene="lacA"
                     /locus_tag="RD444_002409"
     CDS             1916..2341
                     /gene="lacA"
                     /locus_tag="RD444_002409"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDT7549756.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2358..2873
                     /gene="lacB"
                     /locus_tag="RD444_002410"
     CDS             2358..2873
                     /gene="lacB"
                     /locus_tag="RD444_002410"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDT7549757.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2884..3816
                     /gene="lacC"
                     /locus_tag="RD444_002411"
     CDS             2884..3816
                     /gene="lacC"
                     /locus_tag="RD444_002411"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDT7549758.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3819..4799
                     /gene="lacD"
                     /locus_tag="RD444_002412"
     CDS             3819..4799
                     /gene="lacD"
                     /locus_tag="RD444_002412"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDT7549759.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4827..5144
                     /locus_tag="RD444_002413"
     CDS             4827..5144
                     /locus_tag="RD444_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7549760.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5144..6850
                     /locus_tag="RD444_002414"
     CDS             5144..6850
                     /locus_tag="RD444_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7549761.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6992..8398
                     /gene="lacG"
                     /locus_tag="RD444_002415"
     CDS             6992..8398
                     /gene="lacG"
                     /locus_tag="RD444_002415"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDT7549762.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8421..8693
                     /locus_tag="RD444_002416"
     CDS             8421..8693
                     /locus_tag="RD444_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HDT7549763.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8714..9613
                     /locus_tag="RD444_002417"
     CDS             8714..9613
                     /locus_tag="RD444_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDT7549764.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(10085..>10533)
                     /locus_tag="RD444_002418"
     CDS             complement(10085..>10533)
                     /locus_tag="RD444_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7549765.1"
                     /translation="FNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEF
                     DNQAIDELLTTFDINQSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELF
                     DYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT         3303 a         1798 c         2107 g         3325 t
ORIGIN      
        1 tttatgacga cttttggaag taaaaatcca tccattatct ttataatcag gattgaatct
       61 tatatgttgg tgatttaact caatttcctc tttaagaatt tctatagccc ttttagataa
      121 ttggacattt tggtaacttt gtaagttttt tacattacct tcatagctat tttctgcagc
      181 tcctccgtga gtggactgac taatgatgat attttgcttt tcaaaatatt gataggcacc
      241 aatattttgg agttttcgac tctgtaagtc aacagggcca ttagttagaa tacctattgg
      301 gatgcggttt ttatgtagag ccagtagtag ttgatgtatc tcaggaatca tcgtaatctg
      361 atcgagttca tattcatagt cagcttggaa gtctagagct tctgtcctag aaatactatc
      421 gatgccaaat tcttccatag tcttctgaca gcgaaaaatt ctaagctgct caatactgat
      481 gagtttttcg tgtatttagg aaaagcaaca tcagaccaac atctgaaacg tttgtaaatg
      541 ttatctattt gttggatatc aatcgttgga aaacatttct caacgctact tttaaagggg
      601 agttgatggt cataaagagt gtcatcaagg tcaaaaacaa tatttttata agtcataatt
      661 ctagtccgct tttaaaatag tagtgtactg gctaaattcc tctaagtgtt gagggctaat
      721 ctggttatct gtaatgatgg tatcgagttg atctagatta tagaagttaa agaaatcgta
      781 tcgatcgaat ttagtactgt ctactaataa gaatttttct acagcattgt ttagggcaag
      841 ttgttgaatc acaccttcct tgtcactata tgtagcaata gaattatggg taacagcatt
      901 tgcactaaca aaagctttgg caaatctaat tgcttttaaa tttgtcgaag ccattgaacc
      961 tacaaaagct ccagttattt ctctatattc accgccaaga agcaataaat caatggtttc
     1021 gctatcattt agaatcaaga acacagggag actatttgta atgacacgaa ttttataacc
     1081 tttacgacct ttcaattcta atgccagttg ttcaagtgta gttccgggtc caataaaaat
     1141 agtatcgcca tctttgatta aagagcaggc ttttttagca atgtcaattt tttcttttgt
     1201 atttagactt ttcttctcga tatgtgtctt ttcaagtggc tttttagagg aaagtaattg
     1261 tgcaccacca tgagtacgtg ttaaaagtcc atcagcttct agagcatcaa ggtctctacg
     1321 ggctgtcata tcggaaatat ctagttcatc tattatttct tttatggtta tggtcccatc
     1381 aatctttaac atatctaaaa ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt
     1441 ctttcataaa gtaatttttt tccaaagata attctctttt aattgtatca taaaagacaa
     1501 tattttcaag gtaaaacaaa caatttcaaa caaaaacaaa tgttagatga tgaaataaga
     1561 acagtggatt gacgtatatt agcttaggtc agattttgta taagacgaaa ataaagtagg
     1621 acctcttaat cagtaagtta tagaaagtaa aagacttttg taatacttga atagatattt
     1681 cacgtccatt ttgtgatgga ttaaatgaac aaaaatgaac aataatttaa cggtgttatc
     1741 tattttttaa aaaaacaaat aaaaaaacaa aaaataaaca aaaaagtgtt gctttttgtt
     1801 tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga acattttgca
     1861 aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg tcaaaatggc
     1921 tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga aaaattttct
     1981 tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt ttgttgatgt
     2041 taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta ttgtaattga
     2101 tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg ttgcagcaga
     2161 agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac gaatgattac
     2221 ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag gtttcgtcaa
     2281 tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca aaatgtgtta
     2341 aggaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc acagatgaaa
     2401 aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat ttcgggacat
     2461 atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa gcagtagtaa
     2521 gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt aacaatgcag
     2581 tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca gctatatatg
     2641 caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact ggtggtcttc
     2701 ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca gaagaaaata
     2761 aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa gcagatgaag
     2821 atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat taaggtataa
     2881 caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc tctagaaaca
     2941 ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg tggaaaaggc
     3001 ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac aggtttctta
     3061 ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt tagtccagct
     3121 ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga gggtaaccaa
     3181 acagaaattt tggaacaagg accaacagtt tctcatgaag aagcagaagg ttttcttgac
     3241 cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag tttaccttca
     3301 ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga aggagtagca
     3361 gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc ggctaaacct
     3421 actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga agtaacaaaa
     3481 gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat cgaatggata
     3541 gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt ctataaggta
     3601 gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac ggtagctggt
     3661 attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca tgcgatgaca
     3721 ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac taattacgaa
     3781 acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca gaacagaaac
     3841 gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg gcatttgacc
     3901 aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca actgtagctc
     3961 aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct tcttcaatgt
     4021 tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa gcaggtcttc
     4081 ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg cctgactgct
     4141 tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt aagttcttgc
     4201 tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct tatattgaac
     4261 gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc cttgcttatg
     4321 atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct cgcaaagtca
     4381 ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt aaagtagaag
     4441 taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtggtt tacagtaaag
     4501 ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca tacatctacc
     4561 taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct cacgactcag
     4621 gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct gtagaacctt
     4681 acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga ttcgaaaata
     4741 ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat aaagtatagt
     4801 aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc ttagggtttg
     4861 aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt aaagcggctg
     4921 aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc tgtattgcag
     4981 aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag gaacttccat
     5041 acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta ttaaaagatg
     5101 tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata aactcattga
     5161 acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct atcttcgtgc
     5221 tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta tctttatcct
     5281 tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg agaccttctt
     5341 gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg gtactaccgc
     5401 taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga ttaacttctt
     5461 atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc ctgcaaaaga
     5521 aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat ttattgcagc
     5581 ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta ttcgtatgcc
     5641 tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat tcactgtagc
     5701 ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg taaccgttgc
     5761 agaatcaatc ggtaccgttc ttgctcctct cttctcagct gcagatggtt accttggaat
     5821 tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg gtccttcaat
     5881 tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact tgcatcttat
     5941 ccaagctgga caacatgcag ataaagttat cacttctggt actcaaatgt ttattgttac
     6001 catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga tttgtaaatc
     6061 agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg gggttaatga
     6121 gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac cgttcatttt
     6181 cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga acatgaactc
     6241 attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac tcggtactaa
     6301 cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata ctatcattta
     6361 ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt ctggtaaaac
     6421 aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag cagatgccgt
     6481 tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata tcacaaaaga
     6541 aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg ccaatgcctt
     6601 gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta gttacggtgc
     6661 tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg tcgcttcaaa
     6721 cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa aaactgaagg
     6781 agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg ttcaacaaca
     6841 gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca gttccttata
     6901 atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca gaatatcgtc
     6961 tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta aagattttat
     7021 ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg atggtaaagg
     7081 cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag agcctgcaag
     7141 tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg gcgttaatgg
     7201 tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg aagtaaatga
     7261 aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc atgttgagcc
     7321 attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg gagatttctt
     7381 aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg aagagttccc
     7441 agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg gtcaatactt
     7501 ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc aatcacacca
     7561 caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag gttataaagg
     7621 cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag aaaatccagc
     7681 agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct tggatgcaac
     7741 ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc tagctgaaaa
     7801 tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag ctaaagattt
     7861 gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct ttgatggtga
     7921 gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc agattaaagg
     7981 tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact ggattattta
     8041 tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa actacaagaa
     8101 gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata ataccgttta
     8161 tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag acgcaattgc
     8221 ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct tctcatggtc
     8281 aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc aagaacgcta
     8341 tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga tagagtaata
     8401 ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa cacttattta
     8461 gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg attcctgagc
     8521 ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga aaagagtgga
     8581 tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa tcactcgatg
     8641 tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa taataaaaag
     8701 aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc agtttaaaac
     8761 acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc tctggcaagc
     8821 tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg gtagtttacg
     8881 aaatgattgg gctatttata gacctcaaga aagacctttt tttacaggtc ttattcgtag
     8941 acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata atagcgtgac
     9001 ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt ttgaactgag
     9061 agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa cgaacttgga
     9121 aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata gtcctttagt
     9181 agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt cttgctcgat
     9241 accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac cttttttgga
     9301 aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca ttacacttga
     9361 ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag gtcttcgagt
     9421 agattttgat gattttccga acttgattct gtggtctaca actaataaaa gtccattcat
     9481 tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt tcttagaaga
     9541 taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct atgatattac
     9601 tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat actcttacct
     9661 tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt taagtttttt
     9721 gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac agtctggtag
     9781 taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc attaatttgt
     9841 ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga actaagagga
     9901 aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata gcgttttgga
     9961 atggcaacag tttttttgac aaattttata aggtgcagaa cttctttccg tatgctattc
    10021 cgattgtcct tgacaatgag cctcctcgga tgtgacgatc ttcggcagga gctaacagtt
    10081 aaagttagac tgcctcgcta gcgttagcac atccgagctc attatcaagg attcgctgcg
    10141 ccaatctctg gttacggtaa ccaaccggtg tctcgtagcc aagtgtaccg tgcaaccgaa
    10201 ggtggttcca ccaattgaca tagtcaaata actccaaatc caattgttgt aaggtttcaa
    10261 atgtgtattg atagacaaat tctactttca gcgacttata agttgattca gctacggcat
    10321 tatcaaaagg acagccttta tgactcaatg attgattgat gtcaaaagtt gttaataatt
    10381 catcaatagc ttggttatca aactcttttc cacgatcagt atgaaaaatc tcaacctctg
    10441 tcagaggttg tttgatacgg ctaaatgctt tttttactag aacggcatct ttatgttctc
    10501 cacaagaata gccgagaatt tctcgattga aca
//
LOCUS       DAPBAB010000078        10496 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPBAB010000078 DAPBAB010000000
VERSION     DAPBAB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.083"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..360)
                     /locus_tag="RD444_002419"
     CDS             complement(<1..360)
                     /locus_tag="RD444_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDT7549766.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="RD444_002420"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="RD444_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDT7549767.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="RD444_002421"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="RD444_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDT7549768.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="RD444_002422"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="RD444_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDT7549769.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1964..2089)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2240..2872
                     /locus_tag="RD444_002423"
     CDS             2240..2872
                     /locus_tag="RD444_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDT7549770.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="RD444_002424"
     CDS             complement(2874..3779)
                     /locus_tag="RD444_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7549771.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4523)
                     /locus_tag="RD444_002425"
     CDS             complement(3864..4523)
                     /locus_tag="RD444_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7549772.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="RD444_002426"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="RD444_002426"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDT7549773.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="RD444_002427"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="RD444_002427"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDT7549774.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="RD444_002428"
     CDS             6766..7851
                     /locus_tag="RD444_002428"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDT7549775.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="RD444_002429"
     CDS             7848..8912
                     /locus_tag="RD444_002429"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDT7549776.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9161..9964)
                     /locus_tag="RD444_002430"
     CDS             complement(9161..9964)
                     /locus_tag="RD444_002430"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDT7549777.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="RD444_002431"
                     /pseudo
     CDS             complement(9984..10289)
                     /gene="kduI"
                     /locus_tag="RD444_002431"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10271..>10496)
                     /locus_tag="RD444_002432"
     CDS             complement(10271..>10496)
                     /locus_tag="RD444_002432"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDT7549778.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3106 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagcaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     9001 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaacgctt ctgtcccagc
     9061 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caagccgctg
     9121 atttcacaac ctctccacta aacggtgtac taaagctctt ttaaggttgt tttccgatat
     9181 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     9241 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     9301 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     9361 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     9421 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     9481 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     9541 taccgccgcc tttttcgatc atatcccgaa taactgcttt tgaaacgatg aatggggcat
     9601 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9661 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9721 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9781 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9841 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9901 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9961 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
    10021 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
    10081 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac gactaaatgt
    10141 tttgtttcat ctggtttgcc catcatatgg aacacacgtg tatcttcatt ggcataatca
    10201 aaatagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagca
    10261 cttcctggtt ctaaaatcgt ataacccatt tataaatgac agctatcgct gaagcttcta
    10321 ctgctgtaaa tatccccgtc aagataccac cgataatgat gacaatcaat agaaggctag
    10381 gaatcgcctc ttttattatt tttccagcat gagccatctg tcctttttcg acaacagggt
    10441 atttattctt tttagcaacg atgaatgcta ctagcatgat tcctagcgcc cacaaa
//
LOCUS       DAPBAB010000079        10383 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPBAB010000079 DAPBAB010000000
VERSION     DAPBAB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.084"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(52..1518)
                     /locus_tag="RD444_002433"
     CDS             complement(52..1518)
                     /locus_tag="RD444_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDT7549779.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="RD444_002434"
     CDS             1905..2501
                     /locus_tag="RD444_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7549780.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="RD444_002435"
     CDS             complement(2559..3041)
                     /locus_tag="RD444_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549781.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="RD444_002436"
     CDS             complement(3147..3344)
                     /locus_tag="RD444_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549782.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3639..4397
                     /locus_tag="RD444_002437"
     CDS             3639..4397
                     /locus_tag="RD444_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7549783.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4457..4885
                     /locus_tag="RD444_002438"
     CDS             4457..4885
                     /locus_tag="RD444_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549784.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4931..5003
                     /locus_tag="RD444_002439"
     tRNA            4931..5003
                     /locus_tag="RD444_002439"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4964..4966,aa:Gln,seq:ctg)
     gene            complement(5153..5524)
                     /locus_tag="RD444_002440"
     CDS             complement(5153..5524)
                     /locus_tag="RD444_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7549785.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5739..5924
                     /locus_tag="RD444_002441"
     CDS             5739..5924
                     /locus_tag="RD444_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549786.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6007..6195)
                     /locus_tag="RD444_002442"
     CDS             complement(6007..6195)
                     /locus_tag="RD444_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549787.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6219..6482)
                     /locus_tag="RD444_002443"
     CDS             complement(6219..6482)
                     /locus_tag="RD444_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549788.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6640..6897
                     /locus_tag="RD444_002444"
     CDS             6640..6897
                     /locus_tag="RD444_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDT7549789.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6968..7159)
                     /locus_tag="RD444_002445"
     CDS             complement(6968..7159)
                     /locus_tag="RD444_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549790.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7300..7620)
                     /locus_tag="RD444_002446"
     CDS             complement(7300..7620)
                     /locus_tag="RD444_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549791.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7643..8077)
                     /locus_tag="RD444_002447"
     CDS             complement(7643..8077)
                     /locus_tag="RD444_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7549792.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8412..9050
                     /locus_tag="RD444_002448"
     CDS             8412..9050
                     /locus_tag="RD444_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7549793.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9374..9457)
                     /locus_tag="RD444_002449"
     tRNA            complement(9374..9457)
                     /locus_tag="RD444_002449"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9421..9423),aa:Leu,seq:cag)
     gene            9985..>10383
                     /locus_tag="RD444_002450"
     CDS             9985..>10383
                     /locus_tag="RD444_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549794.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSV"
BASE COUNT         3282 a         2043 c         1663 g         3395 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa
     3541 aactaattat ttcctacata ttaaaatata atacttccat ttccaataaa aatacattta
     3601 taataggaaa gataaacagc tgaaaaggga gatttcaaat gaagaaaaaa acgttcaaaa
     3661 aactattgtt tctagggggc acagctgctc ttttattaag cggtacaagt tattacctac
     3721 atatccattc tccagaacat acaatgaaca ctgcacaaaa aagttcaaca caaaaaacga
     3781 aaaagacaaa agagaaagca gaagttttac tagatgttcc attagagagc cagtttgatt
     3841 ctccttcttt agaaaatggc tgtgaagtca cttcgctttc tatgctttta tctttttatg
     3901 gttatgaaac aactaaaaac cagttagctg agcaattgga ttacgtccct gttttcaatg
     3961 cagatgggac tcatggcgat cctaatgaag gatttgttgg tgatatcagt ggcggagact
     4021 gggctatggt cgtctatgtt cccccagttg cctcccttgc tcagcaaatc gtccaaacgg
     4081 attatcacac ttttccaaaa accgatgctc aactcgatga tatcaaaaaa gcactatcta
     4141 aaggaaatcc tgtctggact tctgtgacta tcaattttga agtcccagat gaaactgatt
     4201 ttatgacttg gaccacaaat aatggagaag taagagtcac tcctcttgtt catgcttgtg
     4261 tagtcactgg atatgacaaa gaaaatattt atgtcaacga tccctatggt gtaaaaaatc
     4321 gagcagtacc gatcaacgat ttttcggcga ttttcactgc aatgggtgga cagatgctaa
     4381 ctttggagaa aagctagtaa ttcaaacaaa aatcccttat actcaagata ccaaaccttt
     4441 gaagggagcg tagataatgg aaaaattcac acataaaaaa atggatccaa atgaaattcc
     4501 gattattttt gtccgtgact gcaagggaaa tgttcaaggt aaagtctcta ttaatgagtg
     4561 gaacgagcgt cgcagacctg ctaccctcaa tgaactggaa atcaaattgt atcgtcaatc
     4621 tcttgtttat tacgcagatc aagagtacga gaaagccacc gatttattga aatttctaat
     4681 tgctcgaaca gagtatactc gctttgaata tattgaacgt ttagcaaaca tctatcatat
     4741 tatgaatgaa cctgtcaaag aatatcagtt attggacacc gtcttgtcag tagcagaact
     4801 gattgccctt cctgctggat tagaaaagaa actagtacgc cgccttttac gagtaaagca
     4861 acaactatcc gatcaagaaa aataacttgc gtttttttag gaaagtgcta tgattatttt
     4921 gttgaataaa tgggatgtag ccaaactggt aaggcacttg actctgaatc aagcaattgt
     4981 aggttcgaac cctgccatcc caatttgatg tgcttgctga aaaaatgaac cccctgtaaa
     5041 agaacaatct ttttgcaggg ggattattgt attattttta ctacttgtat ttagtttaca
     5101 taattaaatt atgaataata aaatcgggcg tattcttaat acaaaaactg atttacccaa
     5161 taattgctaa tgctttctcc ggaacttttt ctctaggaac ttcctcatac gtttctatgt
     5221 gtgctccttt attgctaatt ttcaaataat gattttcttt taacacagaa agaccattaa
     5281 attcaaccgt tcgttctttc ccattggcat cagcagcttt ctgcacatac cttgctgtac
     5341 catattcatt tacttcctct ggtttttgtg tctttacata gacctctcct ttttcaacta
     5401 atggatttag ctgatcaagt ataccaggga tttctccata acttccttca gtataatatt
     5461 tcagtccaaa tagcaaaata ccactaatcg ccgccagtcc aatcaaatat ttaatcactt
     5521 tcatctttct acctccttgt tatatattca gtatatgggt cagttaaaga aattccatcc
     5581 gtcccaagtt ggagattttt ctcctacttt agaagcgaga atcgagtgtt tttttagtaa
     5641 aaatgatcgt tatttgatat tattgacgtg tggataaatc agtttttctc gttttgtcca
     5701 tgtaaaaaat catgttgata gcaaaggatg tgttcaaaat gaatcaagaa gaattatttc
     5761 aaaaagtcaa ggaaatgatt aagaacggca atttcgatgg cgcaaaacga tttatcgaag
     5821 aacataaaga acaattagga ccttataaag agaaagccca aaatctttta aaagatgtaa
     5881 atatcgacag tgtcaaaaac aaatttaaaa atctattcaa ataagtaaaa ctttcacttt
     5941 tgtaaaatca aaggaggaca atgctgctgc atcgtcctcc tttggttctt ttctgaatac
     6001 ttcatactaa aataaacgtg tcggatcgta atgataagtc acattgccgt tcagtgaggc
     6061 ttttcccacc acatatttac gattctccaa cttcagccac gcttctcgaa aagccattaa
     6121 ttcttctctt tttacatcaa tcttttcgat ttttccatcg atcaaatctt gaataagttg
     6181 ttcatatgga ttcatttttt tcctccatct gctgtcaatc aataagttgt aaaaataggt
     6241 tcatatgctt ttttggtatc tagatcttct ctgactttga ctgtcgtata aataattttt
     6301 tctactggga tactataata acttccttca ccattattga atcggtgaac gtctttcttt
     6361 gctaaaatac catttatttt attcgtcagt acacgttgtt cgacactgtt acttacaaca
     6421 tagtaaatca cccgaatatt attgatcaaa tgcaaatcga tttctaacac tacatgatcc
     6481 atgaaaatcc cactccttct ttcactttga ttatagcaag tttctcctct aatttcaaaa
     6541 tcgatacaga ttttaccgga taaatccaca aaactgcttg cgccatttcg cacaaagtgt
     6601 tattttgtat catggacttt atttatcgga ggcaatagta tgatcgcgat cggtcaattt
     6661 gttttttata ttcctttttt tattatgata agcattcttt tctattacat aaaatggaca
     6721 aaaaagaagt tttcagtttt actggcctct ttacctgctg tatattttac ttatcaaatt
     6781 ttttctttta ggcattggga aacaacgtct gtattaataa cacacatcat cgaattgacg
     6841 ctttcagtaa tttttcttat tatttggatc tattttttat ataagaatca gaattgaatc
     6901 cctcgcatgt ttaaaaagaa aacgattaaa gatgcagcaa atttctgccg atctttaatc
     6961 gttttttcta ttctgttata ccaaaaaaat gtttgaatcg gtgaaggatc gttccttgtt
     7021 ttaattctat aaattcttgt gcgatcgggt acttttcttc caaattttct ttgttctttt
     7081 cgatcaggct tttcgcttct tcatattttt tatctgcaca tagtgctttt acttcctgta
     7141 atacttttcc tgcttccatt tgcctcaccc tcttgccatt ttattgcttt aagttttatt
     7201 taagttatac gcattattct agtttcatcc caaataacct tttttcattt ttactcctcg
     7261 aactaatcag ttttctaaag gctgattggt tcgtcttttt tatttttcct ccatttcatt
     7321 ctgctcttct gttaatttat ctaatgcttt gattgatgct gcggctcttg cataatcata
     7381 gctgtataat tccataaatt tatcaggcag aacaaaccac gctggatgat aaccgttagc
     7441 tgtgacaggg aatcctttat cttgttggtt atctccatcc catctcagag caacaccttt
     7501 ttttcctttc cactttacta aagcgatcga gtatcccttc tttgtttgtg tgccgtcttc
     7561 aataattgct aaaatttcta ttttttcttt tggcgcacgg acttcttgag gacgaaccat
     7621 tttctattcc tcccttatct cattacttca cgaccaacac actgcaaggg gcatgtgata
     7681 cgacataaga agcggtcgat ccaactaatg cttgttgtat agcacctttt cctgtcgctc
     7741 ccataacgat caaatcaata ttttcttgtc cagggatgac attggcaatc aaatttcttg
     7801 gatctccagt ttcaactatt gcgtggatct cagcaatgcc gtactctttg gcatcattga
     7861 tttttttcag catggctact tctgttcttt gtttttcatc tgcatagatt tttgaaaagg
     7921 cataggcact atgggtcagc tcaactttgt tgatgattga cacgataaat aagtgggctt
     7981 cattgtcctt tgctaagtca agggcttcag cgaacgcttt gatcgattgt cttgatccgt
     8041 caactgctac catgatgttc tggtatcttt tactcattgg aaccgcctcc tttttctttt
     8101 attgtaacat ctcaagcaaa aatacaaaaa atatacgcaa tatgtaaata aaatgatgaa
     8161 caaaagcaat ttctttcaag ttatttatac atttcttttc tcatcagaac acctgttcta
     8221 atcccattat ttctgcatta tgtgaaattc ctcatcattt ttcacatcga gcgaacatca
     8281 ttctgttaca aaaaacgtgg atttgtcata aattgtcatg cttcttttac ctatgaaata
     8341 ttttcacgtg ttaaagtatt cctcgttgaa cacaacaaat aaaacttttt aacgaataaa
     8401 ggagattttt catgacatca cttaaaactt tactttttgg aacaactttg gctgccggcg
     8461 ctgcattctt catgggaacg actgctcatg cggacgaagc ttataccgtt caatcaggtg
     8521 atactctatc aacgatttct caaaaatacg ttggtgataa ctccttgatc aatgccattg
     8581 cagaatcaaa ttcaatttct gatattaacc tgatctactc aggacaacaa ttaacaatcc
     8641 caacagaagg ctctgcacaa gcagctgctg aacctcaagc agccgttcaa gaagcaccag
     8701 tccaagctga acctgttcaa gctgaacaac ctgttgtaca agaaacggtt caaacggaaa
     8761 cacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa actgctgcta
     8821 ctccagcaag tacaagctct gcaaaagaat ggatcgcaca aaaagaatca agcggttctt
     8881 acacagcaac gaacggacgt tacatcggcc gttaccaatt agattcttct tacttaaatg
     8941 gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact tcacgttatg
     9001 gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa gattcattat
     9061 ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt ctttcgcatg
     9121 acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc agtatttctt
     9181 gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa ctgtcccacc
     9241 ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa tttttcctat
     9301 attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct tctctttata
     9361 aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc tcactagcgc
     9421 ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta gtttaggatt
     9481 acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac gccctccctt
     9541 atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg gttggtgaaa
     9601 aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg ggattagtaa
     9661 ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc tatcttcttt
     9721 catttggata ttgattattt cttctgttga ttactatcct tctggaattc tagacgctga
     9781 agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga acttcaaata
     9841 atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga tatgacacaa
     9901 taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa caaatgaata
     9961 aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc agatttaaag
    10021 aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat caacaatgct
    10081 tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa aacgtcttta
    10141 ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga aagtgatgaa
    10201 catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt aagaaacggc
    10261 gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata tgccacacaa
    10321 ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc acttttgtct
    10381 gta
//
LOCUS       DAPBAB010000080        10130 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPBAB010000080 DAPBAB010000000
VERSION     DAPBAB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.085"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..225)
                     /locus_tag="RD444_002451"
     CDS             complement(<1..225)
                     /locus_tag="RD444_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HDT7549795.1"
                     /translation="MENENRTDWTLPRKNLNPKTKQPYKRGRNWWIVVYPESLPENWK
                     EIISTEPVAISPLHDKDVNADGTKKKPHYHI"
     gene            complement(349..504)
                     /locus_tag="RD444_002452"
     CDS             complement(349..504)
                     /locus_tag="RD444_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein repA"
                     /protein_id="HDT7549796.1"
                     /translation="MVEVEKKKVTLSLPVESNDKLEKMAQKYGMTKSGLVTFLINQAD
                     DKGTIFK"
     gene            complement(1048..2259)
                     /locus_tag="RD444_002453"
     CDS             complement(1048..2259)
                     /locus_tag="RD444_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDT7549797.1"
                     /translation="MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNY
                     DLVNRTENYKRDIEQFINDNKSSSRAVRKDAVLINEWIITSDNPFFKDKDDKEIKDFF
                     DTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFNDEHKLSAKTVFNRQALQAVQ
                     DELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKSEVLALSND
                     KAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVI
                     KSYEELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEK
                     ENKSLKNEIKGIYKGFNQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNE
                     TKTRDRDELSR"
     gene            complement(2512..3159)
                     /locus_tag="RD444_002454"
     CDS             complement(2512..3159)
                     /locus_tag="RD444_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-8 chloramphenicol O-acetyltransferase"
                     /protein_id="HDT7549798.1"
                     /translation="MTFNIINLETWDRKEYFNHYFNQQTTYSVTKEFDITLLKSMIKN
                     KGYELYPALIYTIVNIINQNKVFRTGINSEGNLGYWEKLNPLYTVFNKETEKFSNIWT
                     ESNVSFNSFYNSYKSDLLEYKDKNEMFPKKPIPENTVPISMIPWIDFSSFNLNIGNNS
                     RFLLPIITIGKFYSKNNKIYLPVSLQVHHAVCDGYHVSLFMSEFQNIVDSVNEWI"
     gene            complement(3495..3728)
                     /locus_tag="RD444_002455"
     CDS             complement(3495..3728)
                     /locus_tag="RD444_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549799.1"
                     /translation="MSKELKKLVADLQGNMDVVGEFMDSPQSVLDKYNVQGKERTVML
                     AKDSEGLNKLGFSSQLVEGALSGAHSQRCHAPI"
     gene            complement(3780..4991)
                     /locus_tag="RD444_002456"
     CDS             complement(3780..4991)
                     /locus_tag="RD444_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDT7549800.1"
                     /translation="MRILSIFFTNDRKCIIASMLSKTGNYRLYYSLYPFEKKDMICER
                     SFMIVPIGIKKGKLIYKEKNQVNHFYVPNCKASGFLNNVRSIDYNTLLHDTYKNLSLT
                     VTNDKGGNIVFVNQEKLNIPVHFTVKNCYFLDNKTLILLCSSTLNSSMLVYYSIPERE
                     LNYLKMTSQLTFPLSFFEHQIIGEKKIPCLTHINRSDDTVLICLHGGPKYHYNHEYLD
                     LIFDFYHNFNNICLVNFPGSTGYSQKYENELIGNGGVVDVEAVKSVVSYYDSQGFKIK
                     IYGESYGAYIAICLSQFINFSIARIVSVSGFTNLFYMYLCSDSRNLIEKYFSDYRKFS
                     PEKNLAMKELLPIRFLHGSEDKTCPIAQINYFVKKYEGMHLTVLDGFSHYEVDIKKEK
                     IRNKRIVRLLK"
     gene            complement(5003..5809)
                     /locus_tag="RD444_002457"
     CDS             complement(5003..5809)
                     /locus_tag="RD444_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7549801.1"
                     /translation="MMPSQFREQHYLGIKGLKIIVKNELNAFLRNKGLIISQLVQPIL
                     YYIFIVLGINSFVTNIAYEGKTISYADYALTGILGILIISQMTQTIYRITIDKRYGLL
                     ALKRQSGVSPFYYIIGMSMYPTIGICIQSSVLYIIATLFGSSIGLINYLAGIIVVIIV
                     LYFWTSIAALITMKINDYQRRDSIISFIITPLGFTAPAFYLITNVPTFIKVIGYFNPL
                     TYQLYAIRSIVFATPANSLLIIVLVFLATLIMISISSFAVSKMSLVLTER"
     gene            complement(5811..6731)
                     /locus_tag="RD444_002458"
     CDS             complement(5811..6731)
                     /locus_tag="RD444_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549802.1"
                     /translation="MKKNVLDIKDLSLKYGKKFAIKDINLSIFTGEIVALVGHNGAGK
                     SSLFNIIAGIQTPTTGSVEFDQDFIEKRLFATIGFSPQTQVMDWYTNVWDNVYLGSQL
                     AGIDAKTGKLLTEKALDLLSISDLKNKPVDALSGGQQQRVQIARSIVHQPDFYLLDEP
                     TTGLDAETSNRLLSFLKKEAAKGKSVFVSSHDLHLLEEFCDNLVLIDKGRVLYAGDLK
                     NFLIENAPKKSYQITTDRSIHEEIIHKLNEKGIAVTGLDNFQYRFILNQESDLTQLFM
                     DTKEFFRIDEIVKETMSLRDLYIHYREGGL"
     gene            6794..7084
                     /locus_tag="RD444_002459"
     CDS             6794..7084
                     /locus_tag="RD444_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549803.1"
                     /translation="MAERTIDQKIQNVLKKFIDSYKDNRSLTPQTSYLFYDFIILSYH
                     NKRKNRYSISTLSEILLAEGIEANLLINIYAHSLYVLALNDGKQIYDKGFLI"
     gene            7081..8388
                     /locus_tag="RD444_002460"
     CDS             7081..8388
                     /locus_tag="RD444_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HDT7549804.1"
                     /translation="MNYYDRVKRQHGIYFNLLSKYNLHPLKKDHWIYAYKQMYNKKKQ
                     GFKIHLSANTNNAINIAKKFLNYNETKQLDFKIVSTLAKFDIQNTGALGYSQIGKLIT
                     IYPNDDDEFLYTLNELELIFKSDLSPEIPSDFRYMLSSVVNYRYGEIVVDPNFNDLRD
                     KTIPNDIIIPVDDYYIPRLKEIPKHLLLLKVIKKTGKGGTYQCLDLKRNELVLLKQGI
                     HQGDLTIDNVDTINLLSLEKRSLLKLNKENQFPTFIDDFYLENSYFLEINYLNKETLY
                     QFMKKEPLSPEKIKSIILKIISVVKLLHEKYNIIHRDISFDNVLIDEHQEISVIDFEF
                     SYDLKQWNPPSIIMGTPGFYNANRLNIDQYTDVYSIISLLYFMDNFDLYCNYKNDHNK
                     LTLQRNSIDKGTSSFYFIYLQAQYHSARYSLDMLEHDLRTLSV"
     gene            complement(8679..9671)
                     /locus_tag="RD444_002461"
     CDS             complement(8679..9671)
                     /locus_tag="RD444_002461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549805.1"
                     /translation="MSKSNKRGDLEYARLFHTSVKEYKAVVDVLEETTDENRVTKLKI
                     KMMLLRKYISDRENVQIKSIVPKLKTLIPGQATIIAQYYTDFTNETNKKMIHVNALGE
                     EQDVRAVFNDITYGYYLHADFDKVERLRNTNQTFLWVMVDGFIESIEEIIFKLDNLIL
                     DQELTSEMSEPVLPCEPVIRYKEVPENKKNKQSGVWANLIADEITDDALKDIVASMSE
                     DDFKCMLKAQEFMDALGKETVPTVETMRSIVLEERIQDWEDFTRIHEIIAGLKDCGLS
                     TRVEYGENGQEASIKLFREVGEGFIISDPQLVHVPTIELCLNSSGEWRVFGFAI"
BASE COUNT         3353 a         1851 c         1280 g         3646 t
ORIGIN      
        1 tatatgataa tgcggctttt tctttgttcc gtctgcatta acatctttat catgtaatgg
       61 actaattgcc acaggttcag ttgaaataat ctctttccaa ttctctggca aactttcagg
      121 ataaacaaca atccaccagt tacgacctcg cttataaggc tgtttagttt ttggatttaa
      181 attctttcta ggcaatgtcc aatctgttct attttcattt tccatatttt tctacacctt
      241 cctagttact ctactaggaa aacaagggaa aatttgataa acttgagttg cgaaatcaaa
      301 gaaaaccaaa tcttgcgagc ccttgctatc ttcctagcag ggcttttttt atttaaaaat
      361 agtcccctta tcatcagctt gattaattaa aaatgtaacc aaacctgatt tagtcattcc
      421 atatttttgt gccatttttt ctagtttgtc attcgattca acaggtaaag ataaagtcac
      481 ttttttcttc tcaacttcaa ccacaaaatg cacctcaatt ctcttcaaaa tctcttttac
      541 actgtaatta tattatacat acgacaggaa tacaagattt tacaaaaaac tttttttcac
      601 ctcgaaatag gtgcttaaaa cgttcatata aaggtattta caagacattt aatcttttta
      661 tctatttgga caatggacaa tggacacatg gggggtgtcg tagtgccccc atgttttttc
      721 gctccaaaaa ttcccatgtt ttttcgcccc ctgctcccac taaatttcca aataataaaa
      781 ccgtgggcta ttcgcccaca cccaacaagc gcaaagcgct tgaccttgcc tctgaaaagc
      841 aacttgataa ctgcattcgt ttctttccgt cgtcttagca aaagctaaac aactcaaaaa
      901 aagagatcaa gggtaaatac actcactacg ttcgcccttg acctctttta tttcgctgtt
      961 ttttacgcat ttgccgacga aaagtaaaca atatgcagaa ttaccctagt tttgataaaa
     1021 aagcgcttag aacgcaaata tgagcttcta acgacttaat tcatccctat ctcgtgtttt
     1081 agtttcgttt ctatgaattt tttcaaactc tccacctttt acaaactctt taacttcgct
     1141 cataaggtta ttcatcagct ttttagcttg tccaacagtt gcaccaaggg tattttccat
     1201 aaactggtta aaacctttgt aaatgccctt aatttcgttt ttaagcgact tattctcttt
     1261 ctctaattct tcaagtgttt caatattctg attactctta cgtaccaacc tattgtaatc
     1321 tttcgctaat tcgcgatatt tctctttaac tctagtgatt tctttaggta aatcagtttc
     1381 cgcataattt tttagctttt catttgcttt atatagctct tcatagcttt taatcactgc
     1441 atttaattta ctttcaggga taattacatt tcccgttttt cgttcttcat ttttcataaa
     1501 ttcatgtcgc agcttaccaa tcccaatggt ttcgccactt ggtactttga ccgttttcgt
     1561 ttcgtctttt aaatctaaac tttctttctt gattgttggt gctttatcgt tcgataacgc
     1621 taaaacttct gactttcgct gttccagcgt ggtagttatc tcttttaatt cgtcctttgc
     1681 ctttttatat tcaggcaccg ttaaattttt acgctctgac cctttttcgc cacgttcaag
     1741 ctcaaaacca cgctcattta aatactttgg caattcgtct tgtaccgctt gtaaggcttg
     1801 acgattaaaa acagtctttg ctgatagctt gtgttcgtca ttaaacggca caattcctaa
     1861 gtgcatatgc ggtgtcgttt cgtctaagtg aacttgagcg tagcggatat tattatcgcc
     1921 aaatttgtcc gcaaaatatg atttcgcagt atcaaaaaaa tctttaattt ctttatcgtc
     1981 tttatcttta aaaaatgggt tgtcagaagt tatgatccat tcgtttatta atacagcatc
     2041 ttttctgaca gcacgactac ttgatttatt gtcgttaata aattgctcaa tatctcgttt
     2101 ataattttct gtccgattga ccaaatcata atttaaatgc gaccgttcaa catcaatatc
     2161 tttgttggaa tgattgtctg tatttctctg attatgatta cctaccccga ctaaatttcc
     2221 tgattttacc ttctgcattc tcgccactgc aaatgacatg taaacactcc taacttcggc
     2281 acaacttcca tgtaaagtat aacccactag acttatttac tacgtttcat aagtcattaa
     2341 tgcgtgtgct ctgcgaggct agtttttgtg caagcacaaa aaatggactg aataaatcag
     2401 tccataagtt caaaaccaaa ttcaaaatca aaacaccagc aaccaaaaat ggtagttgct
     2461 ataaacttat aaatatcata attacttaag tttatgaaag ttattttaga tttaaatcca
     2521 ttcatttaca ctatcaacta tattttgaaa ttcactcata aataatgaaa catggtaacc
     2581 atcacatacc gcatgatgaa cttgcaatga gactggtaaa tagatcttat tattcttact
     2641 ataaaattta cctattgtaa taattggcaa taggaatcta ctattattac caatatttaa
     2701 attaaatgaa ctaaaatcaa tccaaggaat catcgaaata ggaactgtgt tttcaggtat
     2761 tggtttttta ggaaacattt catttttatc tttatattca agtaagtcac tcttataact
     2821 attataaaaa gaattaaaac taacatttga ttctgtccaa atgttagaaa atttttcagt
     2881 ttctttatta aagactgtat ataaagggtt taatttttcc caataaccca aatttccctc
     2941 actattaatt cctgttctaa atactttatt ttgatttata atatttacaa ttgtataaat
     3001 caaagcagga tacagttcat atcctttatt ttttatcata cttttaagta aagtgatatc
     3061 aaattcttta gtaacactgt aagttgtttg ttgattgaaa taatgattaa aatattcttt
     3121 tctatcccaa gtttccaaat taataatatt aaaagtcata tataattttc ctcctaaatt
     3181 tcatcttatc tctttgtatt taggaggaat aatcctctga ttttttcata tattgtgtca
     3241 cctcttaaac gttaattata ctaaagtaac attttgtata aaacaaagtt actttacttc
     3301 caaaatctaa attcacgttg ccaaaaatca atctgctttt gcaattgttt ttcgttcgct
     3361 tttaaagtcg atttcattaa acttcaactt tgcaaataaa tcagactatt taagtataat
     3421 tgattgactt aacaatgcta ttagcacaat aatatccctc tacataaata aagtagagga
     3481 atatggaaat atttttatat tggtgcatga catctttgac tgtgagcacc agataaagct
     3541 ccttcaacta actgactcga aaaccctagt ttattcaaac cttcgctatc ttttgccaac
     3601 atcacagttc tttctttacc ttgtacattg tatttatcca agacactttg aggactatcc
     3661 ataaattcac cgacaacatc catatttccc tgtaaatctg caaccagttt tttcaattct
     3721 ttagacataa tactgcaccc ccgtaaagtt tataattata tgataacatt catcagtaat
     3781 tacttcaata atctgactat tcttttattt ctgatttttt ctttttttat atctacttca
     3841 taatgagaaa aaccatctag tactgtcaaa tgcattcctt cgtatttttt tacaaaatag
     3901 ttaatttgtg caatcggaca agttttatct tcacttccat gaagaaatct tataggtaat
     3961 aattctttca ttgccagatt tttttctgga gaaaatttcc tataatcaga aaaatacttt
     4021 tcaataaggt ttctcgaatc tgaacaaaga tacatataaa ataaatttgt aaatccacta
     4081 acagagacta ttcttgctat actaaaatta ataaattgag agagacagat tgcgatatac
     4141 gctccataac tctcaccata tatttttatt ttaaatcctt gagaatcata atagctaacg
     4201 acagatttta cagcttcaac atcaacaaca cctccgttac ctattaactc attttcatat
     4261 ttttgggaat atcctgtact tccaggaaaa ttcaccaaac aaatattatt gaaattatga
     4321 tagaaatcaa agattaaatc taagtattca tgattatagt gatatttagg tcctccatgc
     4381 aaacatatca gaactgtatc atcacttcta ttaatgtgag ttaaacaagg aatttttttt
     4441 tcaccaatta tttgatgttc aaaaaaagaa agagggaaag taagttgact agtcatcttt
     4501 aaataattta attctctttc aggaatactg taataaacta gcattgagct attcaaagtt
     4561 gaactacata aaagaatcaa tgttttgtta tcaagaaaat aacaattttt tacagtaaaa
     4621 tgtactggaa tatttaattt ttcttggttt acaaatacaa tattgcctcc tttatcattc
     4681 gtaacagtta aactcaaatt tttataagta tcatgcaaca gagtattata atcaattgat
     4741 ctgacgttat ttaagaaacc agaggcttta caattaggca cataaaaatg attcacttga
     4801 tttttttctt tatagataag ctttcctttt tttataccta ttggcactat cataaaagat
     4861 ctttcgcata tcatatcttt cttttcaaat ggatacagcg aataataaag tctataatta
     4921 cctgttttac ttagcatact tgcaattata cattttcgat cattagtaaa aaaaatgctt
     4981 aggattctca ctcagtcacc ttctatcgtt ctgtcagcac taaactcatc ttggaaacag
     5041 caaatgacga aatacttatc ataatcaatg tggctaaaaa tactaaaacg atgattaata
     5101 aactatttgc tggtgtagca aatactatag accttattgc atatagttga taagttaacg
     5161 gattaaagta accaataact ttaataaatg tagggacatt tgtaattaaa taaaatgcag
     5221 gagctgtaaa ccctaatgga gtaataataa acgaaataat tgaatctctt cgctgataat
     5281 cattaatttt catcgtaatc aacgcagcta tggatgtcca aaaatacaag acaattatca
     5341 caactattat ccctgccaaa taattgatta aaccaattga gcttccaaac aaagtagcta
     5401 taatataaag tacactagac tgaatacata tgcctatagt aggatacata ctcattccta
     5461 taatgtaata aaatggtgaa actccacttt gacgtttcaa agctaataga ccatatcttt
     5521 tatctatagt aattcgataa attgtttgag tcatttgtga aataatcaat attcccaaaa
     5581 tcccagttaa agcataatcg gcataagaaa ttgttttccc ctcataggca atatttgtga
     5641 caaaactatt gatacctaag acaataaaaa tatagtacaa gatcggttga accaattgtg
     5701 atatgatcaa tcctttgttt ctaagaaacg catttagctc attttttaca atgattttta
     5761 atccctttat tcctaaataa tgttgctccc taaattgact tggcatcatt ttaaagaccc
     5821 ccttctctat aatgaatgta taagtctctt aatgacatag tttccttaac aatttcatct
     5881 attctaaaga attcttttgt atccataaac aattgtgtta aatcactttc ctgatttaat
     5941 ataaatctat actgaaagtt gtctaatcct gtcacagcaa tacctttttc atttaactta
     6001 tgaataattt cttcatggat tgatctatca gtagtaatct ggtaactttt tttaggcgcg
     6061 ttttcgatca agaaattttt tagatccccc gcgtataaaa cccttccttt atctatcaaa
     6121 actaaattat cacaaaattc ttctaataaa tgtaagtcat gtgacgacac aaaaactgat
     6181 ttccctttag ctgcttcttt ttttaaaaat gacaacaacc gattagatgt ttctgcatct
     6241 agtcctgttg ttggctcatc taacaaatag aagtcaggct ggtgaacaat ggagcgcgca
     6301 atttgaacac gttgttgctg tcccccagat agtgcatcta caggtttatt ttttaaatcc
     6361 gatatactta ataaatctaa ggctttttcg gtcaataatt ttcctgtttt tgcatctatt
     6421 cctgctaatt gactgcctaa gtaaacgtta tcccacacat ttgtgtacca atccatcacc
     6481 tgagtctgcg gtgaaaaacc tatagttgca aataatcgtt tttctataaa atcctgatca
     6541 aactctactg aacccgtcgt cggagtttga atcccagcaa taatattgaa taagcttgat
     6601 ttacctgctc cattatgccc aactaatgct actatttcac cggtaaatat ggaaagatta
     6661 atgtctttta tcgcaaattt ttttccatat ttaagactta aatcttttat atctaataca
     6721 ttttttttca ttactaaact ccttttctaa ggtataatta attaaatctt acttaaaaga
     6781 gagggattct aatatggctg aacgaaccat cgatcagaaa attcagaatg ttttaaaaaa
     6841 atttattgat tcctataagg ataatcgaag tctcacaccc caaacctcct atttatttta
     6901 tgattttata attctatcct accataataa aagaaaaaat cgttacagta tttctacgtt
     6961 gtcagaaata ttattagcgg aaggtattga agcaaattta ttaataaata tatacgctca
     7021 ttccctttat gtactagcat tgaacgatgg caagcagata tatgataaag gatttttaat
     7081 atgaactact atgatcgagt taagaggcag catggtatct attttaattt attatctaaa
     7141 tataacttac atcccctaaa aaaagatcac tggatttatg catacaaaca aatgtataac
     7201 aaaaaaaaac agggatttaa aattcattta agtgctaata ctaataatgc cattaatata
     7261 gctaaaaaat ttttaaacta taatgaaaca aaacaactag actttaaaat cgtttctact
     7321 ctagcaaaat ttgacataca aaatactggg gcattaggat attctcaaat aggaaagtta
     7381 atcactattt atccaaacga tgatgatgaa tttctttata cacttaatga gctagaactg
     7441 attttcaaat cagacttatc tcctgaaatc ccttcagatt ttagatatat gttaagttct
     7501 gtggttaatt atagatatgg agaaatagtt gtagacccta atttcaatga ccttagagat
     7561 aaaactattc cgaacgatat aattatacct gtggacgatt actatattcc cagactaaaa
     7621 gaaattccta aacatttatt actattaaaa gttataaaga aaactggtaa aggtggaaca
     7681 tatcaatgcc ttgatttaaa aagaaatgag ctagtactat taaaacaagg tatacatcaa
     7741 ggtgatttaa caatcgataa tgtggatact attaatcttt tatctttaga aaagaggagc
     7801 ctcttaaaac taaataaaga aaatcaattt ccaacgttta ttgacgattt ttatttagaa
     7861 aactcttatt tcttggaaat aaattatcta aataaagaga ctctatatca atttatgaaa
     7921 aaagaaccgt tgtctcctga aaaaataaaa tcaattattt taaaaattat ttcagtagtt
     7981 aaattgctcc atgaaaaata taatattatt catagggata tttcattcga taatgtttta
     8041 attgatgaac atcaagaaat ttctgtgatt gattttgaat tttcctatga tttgaaacaa
     8101 tggaatccac cttctattat tatgggtact cctggatttt ataacgcaaa ccgattaaat
     8161 atagaccaat atacagatgt ttacagcatt atcagtctat tatattttat ggataatttc
     8221 gatctatatt gtaattataa aaatgaccac aataaattaa ctttacaaag aaattcaata
     8281 gataaaggta ctagttcttt ttattttatc tatttacaag ctcagtatca ttccgctagg
     8341 tattcactag atatgcttga acatgattta aggaccctaa gcgtttaaat tgttatagtt
     8401 aaactcaact aatctgcaca acttaaatgt cctctatcaa taaattattc tattgaataa
     8461 cgctaagtcc aacagggtat actctgttgg actaaaaata caaacacata tgtgataaaa
     8521 gtatattttt attatcaacc ctattccatg ctgtttcaaa taaatttaag ttagatttta
     8581 ttcttgcaat atgtgagaac cagtagattc attaaatacg agggtatata tttgagaacc
     8641 atgctattct attttatcct tatggaagaa gattctggct aaatggcaaa accaaacact
     8701 ctccattcgc cgcttgaatt aagacataac tcaatagttg gcacatgtac gagttgtgga
     8761 tctgatatta taaatccctc gcctacttct ctgaataact ttatactcgc ttcttgccca
     8821 ttctctccat actctactct cgttgacaac ccacaatcct ttaaacccgc aataatttcg
     8881 tgaattcttg taaaatcttc ccaatcctgg attctttctt ctaaaactat tgaacgcatt
     8941 gtttcaactg ttggcacagt ttctttcccc aacgcatcca taaattcttg tgctttaagc
     9001 atacatttaa agtcgtcttc agacatactt gcaacaatat cttttaaagc atcatcagta
     9061 atttcatccg ctataagatt tgcccacaca cctgattgct tatttttttt attctctggt
     9121 acctctttat accgaataac aggttcgcat ggcaaaactg gttcactcat ttccgaagta
     9181 agttcctgat ccaggatcaa attatccaat ttaaaaatta tctcttcaat actttcaata
     9241 aacccatcga ccatgaccca caaaaaagtt tgattcgtat ttcgcaatct ttcaactttg
     9301 tcaaaatctg catgaagata gtaaccgtaa gtaatgtcat taaacacagc cctaacatct
     9361 tgttcttcac ctagagcgtt tacatgaatc atctttttat ttgtttcgtt agtaaaatca
     9421 gtatagtact gagcaataat tgtagcttga ccgggaatca aggttttaag tttaggtacg
     9481 atacttttta tttgtacatt ttctctatca gatatatatt ttctgagcaa catcattttg
     9541 attttgagtt ttgtcactct attttcatca gtcgtttctt ctaaaacatc cactacagcc
     9601 ttgtattctt ttactgaagt atggaacaat ctcgcatatt caaggtcgcc tcttttatta
     9661 ctttttgaca tttcttttat cctcttactt ttctaaaatt aaactaactt ttaattcttt
     9721 caattttcct ctatctaagt tatactatat gcaaacaagg agctgcgctt taacatagcc
     9781 ccttaaagcg tagtccgttt aagacggcag ctgaatttaa tacgtaaata aatccgtcaa
     9841 ttctcgctaa agtctatgac gggtttattt tttgttgttt ttgctaattg ttagcataat
     9901 gccaactaaa gcaaggacga atgatccaaa ttgaatcatc aaaccaattg cttcagcaac
     9961 agacaaaaaa tgctactccc ttctacgaag taggaaaatg cacataagca tcaccccctg
    10021 agtagccacc gtcataaact cactacaaat atattatagc atatctagct aaatcctctt
    10081 attataattt tgctcatgtt tagaattcat attttccttc atcatcattg
//
LOCUS       DAPBAB010000081        10002 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPBAB010000081 DAPBAB010000000
VERSION     DAPBAB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.086"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..301
                     /locus_tag="RD444_002462"
     CDS             <1..301
                     /locus_tag="RD444_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDT7549806.1"
                     /translation="INLKDICREVAKELKGCERVDGKYDFDRFYQEKGDINAQILFSY
                     KAEQYYKGYKNHEHEFVPEVRKDWNDQLKHEIQQQEIRKQKRAMLFQQNRQQERE"
     gene            312..2516
                     /gene="topB"
                     /locus_tag="RD444_002463"
     CDS             312..2516
                     /gene="topB"
                     /locus_tag="RD444_002463"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7549807.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLENLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGVPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIEILKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPRCQEQEVILYQKVAACTSEVCDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            2532..2681
                     /locus_tag="RD444_002464"
     CDS             2532..2681
                     /locus_tag="RD444_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549808.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            2756..3349
                     /locus_tag="RD444_002465"
     CDS             2756..3349
                     /locus_tag="RD444_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDT7549809.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            3362..4261
                     /locus_tag="RD444_002466"
     CDS             3362..4261
                     /locus_tag="RD444_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDT7549810.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTILKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4264..4749
                     /gene="ssb"
                     /locus_tag="RD444_002467"
     CDS             4264..4749
                     /gene="ssb"
                     /locus_tag="RD444_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7549811.1"
                     /translation="MINNVTLVGRLTKDPDLCYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMANLARKGSLTGITGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5117..5377
                     /locus_tag="RD444_002468"
     CDS             5117..5377
                     /locus_tag="RD444_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549812.1"
                     /translation="MNNYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            5818..6024
                     /locus_tag="RD444_002469"
     CDS             5818..6024
                     /locus_tag="RD444_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDT7549813.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            6024..6275
                     /locus_tag="RD444_002470"
     CDS             6024..6275
                     /locus_tag="RD444_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549814.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            6290..6727
                     /locus_tag="RD444_002471"
     CDS             6290..6727
                     /locus_tag="RD444_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549815.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            6720..7427
                     /locus_tag="RD444_002472"
     CDS             6720..7427
                     /locus_tag="RD444_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549816.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            7808..7987
                     /locus_tag="RD444_002473"
     CDS             7808..7987
                     /locus_tag="RD444_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549817.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            8328..8591
                     /locus_tag="RD444_002474"
     CDS             8328..8591
                     /locus_tag="RD444_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDT7549818.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            8585..8935
                     /locus_tag="RD444_002475"
     CDS             8585..8935
                     /locus_tag="RD444_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7549819.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(8959..9573)
                     /locus_tag="RD444_002476"
     CDS             complement(8959..9573)
                     /locus_tag="RD444_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7549820.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         3620 a         1663 c         1999 g         2720 t
ORIGIN      
        1 tattaatttg aaagatattt gtagagaagt cgcaaaagaa ttaaaaggtt gtgaacgcgt
       61 ggatggtaag tacgattttg atcgttttta tcaagaaaaa ggggatatta atgctcagat
      121 tcttttctct tataaagcag aacaatacta caaaggttat aaaaatcatg aacatgaatt
      181 tgtacctgaa gtgagaaaag attggaacga tcaattgaag catgagatcc agcaacaaga
      241 aatcagaaaa caaaaacgtg ccatgttgtt tcaacaaaac agacaacaag aaagggagtg
      301 aaatcgtcat gatgaaaacc gttattttag cagaaaagcc gtcacaagcg aaagcttatg
      361 ccgatagctt ctctaaagcc acccgaaaag acgggtattt tgaaatccaa gatcgattgt
      421 ttcctggtga aactgtgatc acgtatggtt ttggtcattt agtcgagtta gattcgcccg
      481 atatgtacga tgaaaattgg aaacaatggt cattagagaa tttgcctatt ttccctacac
      541 attatcacta tcatgttcct aaagataaga aaaagcaatt caaggtggtg aaacagcaac
      601 ttcaatcggc cgacaccatt attattgcga cagatagcga tcgtgaaggg gaactgatcg
      661 cttggacgat cattcaacaa gcaggcgccg atcatggaaa gatatttaaa agactttgga
      721 tcaactcctt agaaaaagaa gccatttatc agggattcca gcaattacga gacgcagaag
      781 agacctatcc caaatttgaa gaagcacaag cacgccagat cgctgattgg ttgatcggca
      841 tgaacggcag cccactgtat agtttactct tgcagcaaaa aggcgtacca ggaagttttt
      901 ccttaggcag agtccaaacc ccaacgcttt acatgattta tcagctgcag gaaaagatta
      961 gaaacttcca aaaagagcct tattttgaag gaaaagccca agtaatcgct caaaatggcg
     1021 catttgatgc gaagcttgat ccgaatgaaa cccaagcgac gcaagaagca tttgaagatt
     1081 atttaaaaga aaagggggtt caattgggaa aacagccagg tacgatccac caagttgaaa
     1141 ccgagaaaaa aagtgcggca agtccacgtc ttttttctct atccagcctt caatctaaaa
     1201 tgaatcagct catgaaagct agtgccaaag atactttgga agccatgcaa gggttgtatg
     1261 aagggaaata tctaagctac ccacgaacag atacccctta tattacagaa ggggagtatg
     1321 cgtatctgtt ggatcactta gacgaataca aacacttttt aaaggccgaa gccattccta
     1381 ctcctattca tacaccgaat agtcgatatg tgaataataa gaaagtccag gaacattacg
     1441 cgatcattcc tacaaagacg gtcatgacgg cagcggcttt tgaacaatta agtccactgc
     1501 agcaggccat ttatgaacaa gtgttaaaaa caacggtggc catgtttgcg gaaaaatata
     1561 cctatgaaga aacaacgatc ttgacgcaag ttcagcagct tcagttaaaa gcaatcggaa
     1621 aagtgccggt agatctaggt tggaagaagt tatttggaaa agagagcgac ggaaaagaga
     1681 aggaagaaga gccgctactt cctaaagtga caaaaggaga aatggtgacg gtagatctac
     1741 aagtccttga aaaagaaacc aagccaccac aaccttatac cgaaggtacg ttgatcactg
     1801 cgatgaaaac agctgggaaa accgtggata gtgaagaagc gcaatccata ttgaaagaag
     1861 tagaaggaat cggtacagaa gcgacacggg caaatattat tgaaatcttg aaacaaaagg
     1921 aatatatcaa ggtagagaaa aataagctcg tggtgacgaa taaagggatt ttgctttgcc
     1981 aagccgtgga aaaagagccg ctgcttacga gcgccgaaat gaccgccaaa tgggaaagct
     2041 acttattaaa gattggagaa cgaaaaggaa cacaaaccac ttttctagct aatatccaaa
     2101 aatttgtctc tcacttattg gaagtcgtac cagggcaaat ccaatccact gattttggat
     2161 cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga agccgcgcgt catttaggaa
     2221 tttgtccaag atgccaggaa caagaagtca tactttatca aaaggtggca gcatgtacgt
     2281 ctgaagtctg tgattttaaa ttatggacaa cgattgccaa aaagaaactc acagcgacac
     2341 agctgaagga gattattcaa aatagccgaa caagccaacc agtgaaaggg ttaaaaggtc
     2401 agaagggttc gtttgaagca acgattgttt taaaagaaga cttcactaca ggctttgaat
     2461 tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg acgcactaca aaataatgga
     2521 gggttaatct tatgcgtaat acaacacaca aacaacaaga aatcgaaaat caaaaagcca
     2581 aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg aagactgcag caattaaaga
     2641 accagcagcg gattcaaaaa gaaattgaat tagatcgata gacattaacc ttgttacaaa
     2701 tacactatca tgatatattt aatgtagtgt atttgtaaca aaagggggga agtaaatgca
     2761 agagaaactg aaagaattgt tggctattcg cgatggaaat ttatctatga aggaagccag
     2821 aaaattagga atcgcagcaa ccactgtaca aagattggtg aatcaaggaa aattgatcaa
     2881 agttgatcgt ggttattatg tagaggatgg tcatggaata gatgatttat tttggcttca
     2941 gcaacgattt tccaggggaa tcttctctca tgaaacgact ttagatattt accagctttc
     3001 tacgaatatg ccgaaattca ttcacttaac ttttccccat ggatataacg ttgatcgttc
     3061 aattacaaaa gagcagcagt tacaatttca tttcgtgaag aaagaagttt atgaactagg
     3121 aaaagtagag ggaacttcct ttcaaggaaa tcctattact atgtatgata aagagcgtac
     3181 actttgtgat atttggaatc cgagatatga aattgaatat gaaatgaaat tagaagcatt
     3241 aaaagagtac atggaagacc cattgagaga tccaagtaaa ttaagagatt acatgacaaa
     3301 actacatgta gaaaaagaaa tgaaatacca catgcaaagc ttatattgat ggaggaaaaa
     3361 gatgacaccg acacaatttg cagcaaaaac aagaaattta gcaaagagta caggattaaa
     3421 tgcacagtta gtgcaacggc attttatgat ggatcgtcta attgaacaaa tttctgaatc
     3481 aaagtacaaa aatgacttca ttttgaaagg cggcttttta ttaggatcaa aatatggaat
     3541 cgatagaagg acaacaattg atatcgatac gacatttaga aatagcaaat taactgaaga
     3601 aaaattgaag agcattttta atgatttaac tagaaaaccc acaaaagaag gcattgtttt
     3661 ctctattcaa ggaatgaagg aaacaagaga agccgatttt tatcctggat ttcaaattaa
     3721 attggtggct aatttagaga aagtacgtgt accgtttaaa atggacgtga caacaggaga
     3781 ttctatttat cctgaagtag atacacacta tcaccaatta atgtttgaag ataaaaaagt
     3841 ggagattcca gcttatccaa cagaacaaat tattgcagaa aagctaagtg ctacgttcag
     3901 ctttggcaca gataattcga gagcaaaaga ttactacgac ttatttacca ttctgaaact
     3961 agaaaatatc gatacgaaag agttatacaa aagtgttcga aatacattta caaaacgtgg
     4021 agatacaaag cctttatcct tttattatga aagagaaatg gataagatca ggggaaacga
     4081 ctatttggaa aagcagtggg aaaaatatcg ctcagttaac acgtttgcga attccatttc
     4141 atttacagat acagtcaacg aaattgatca cctaatgaaa agtgtgatca aaattgaaaa
     4201 tcaagaaaga aaaaggcagt ttaaaagatc acaacaaatg aatcaagaaa tggagagata
     4261 aagatgatta ataatgtaac tttagtagga cggttaacca aagatccaga tttatgttat
     4321 accgcaagtg gcacagcagt ggctactttt accctagccg ttaatcgtaa tttcacgaat
     4381 caaaacggca atcgagaagc tgattttatc aactgtgtga tttggcgtaa acctgcagaa
     4441 accatggcaa acttagctag aaaaggtagt ttaacaggaa ttacaggacg gattcaaaca
     4501 cgaacttatg acaaccagca aggacaacgg gtgtacgtaa cagaagttgt ggccgataac
     4561 ttccaattat tagaaagcaa agcagcaaca gaaagccgta tgcacgctga tcaatcgtcc
     4621 agcagtccaa gtcctaccac ctttgaacaa cgggatgtag ctacgcctaa caacaatgga
     4681 ttgaatgctt cacagaatcc atttggtgga caatcaatcg atattagcga tgatgatcta
     4741 ccgttctaag tagaatattc tattgtatcg gttgcatttg tgactaatta tgatataatc
     4801 aaggtaacaa aaaacaggtt catattacga ccgaaaacca ctagcgggct ggctagtggt
     4861 tttcttttac gttcttttca gattaacggg taggcttact gtcttttttg gcaattttgc
     4921 aatcaagcca tttgcagata tagtgggctg ctacttctgc cgcaattgca aaaataaaat
     4981 caaaaaacag actcattaat tacgacctcc tccccttgcc agattaggcc ggggtagcag
     5041 acagcaaaac tattatagca taattgaaat atccaaatga tttgattttt gctataattt
     5101 tatcgaggtg attgtgatga acaactatgt tggtttgctt gataaaatcc gagtaataaa
     5161 aacgcaaccg ttattggttc gctttacttt acaaacgatt catgaatcga ttaattgtgt
     5221 agttgcagat attgaaatta tcgataaatt gctaattatg gatgatggaa aatacaatat
     5281 tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt attgctagca tgtatgttag
     5341 aaatcccgat cattttacgc gatcgatggg catataaata aaagaagagt tgtagtccta
     5401 aatgaggatt gcagctcttt ttgttttaca tagaaacagg aaaagacctt tttaggaaga
     5461 cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc aaccgccacc gccgttcgag
     5521 aagaaaggaa gtgaacgctg cacgttcact aagatatggt gtcttgcaaa gcaagacaaa
     5581 atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct ttattgattt
     5641 gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa gggtaaatgg
     5701 cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt attctggcca acaaatcaat
     5761 aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga atcaaacatg
     5821 cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa ctatcatgca
     5881 ccacatactg ttgaatgcga ttgtggagaa agagcgaaat gtatcaaaag ctatcgtttg
     5941 tatcaatgtc caacatgcgg gaaaaaatat accttgtctt atggcgatta cattttgatt
     6001 gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa attattgact gtaaaagaag
     6061 tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa gctttactta
     6121 ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact atgctatttg
     6181 aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg gaaacttatg
     6241 aaatcgaata ttggttaacc aaattagaga actaaaaagg agaaaaaata tggctcaaaa
     6301 ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat
     6361 gacgttccta taccatgaaa cacgtgtacc caaaaaacat tacaaggaaa aattgtcagt
     6421 cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat ctcattgcga cgtactatcg
     6481 aacactggaa gaactgaaaa aacaaaatgg aaaatggttt ttccaagcct tactatgctt
     6541 ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa tatcaagcct tagaactgac
     6601 ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg gctagcgact ggctagatac
     6661 ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt gaatcggagg agaaagataa
     6721 tgaataaggt ggtttatcag ctaacaacag aagatgtttc gcaattttta acaaacaaag
     6781 aacacttgaa tgaagtaatc agacggattc aaaaagactt tagttgtatg gaagaaatga
     6841 agtactttat tcaaagagtg gaagaacagt tgtatatgga aagcgaaaca agtcaaacga
     6901 aggtcacagt cagccataat catttagacg tgtataaacg tcgtcctgaa acggtaaaaa
     6961 aaggaacaga gcttctacga ctagaaacag aaaaatacat catgaatgtg tacctagaaa
     7021 ggagtgagct ggtcgaaaac tttgtcagct acacgatttg taggaaacac tattactcag
     7081 aatcagaata ttattcaatt ttcgagagca tggaattaat ggaagaagaa atggaagagc
     7141 taaacagcaa aaatgcgacg gtagacgatt attaccttgt atttactagg tttttgacgt
     7201 gtgggacgga atctatcgtg ttaatgggaa tggattatga actcgctgta cccgaagcat
     7261 ttttgaggga atatgtagaa gaagtagata acccaggcga cgactggtac acgactttaa
     7321 ctgatttttt agacacctac acttacgacg atacagaaga aatccaaaat gaacttgacc
     7381 gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg taaataacca tttataccga
     7441 aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa ttaagggaat ggttcttatt
     7501 tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag cgggcaaagc
     7561 ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc tattgttcgt
     7621 actgtttgtt ggcgagatac ttatgcgtca tgctgcagct gtacatttaa acttcgtagt
     7681 attcttagga atactggtat tttgtttgat cttttggcga tttgtattag cccttgtgag
     7741 tctactattc aagatgattg cagccatatt tttcataggg ttatttcgat gaaaggagag
     7801 agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag atgaaaagag
     7861 acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta agattatgtg
     7921 tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac cagcactaaa
     7981 aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt gttcctacta
     8041 tttagaatat tttctatgtc ctttttattc cattttgtat caataactat gaaatcttta
     8101 aagtatccag aagagattat ttttagatca atttttttct tacctgattc taaagatttt
     8161 gttttcttaa acatattatg atttcctttc gactattttc atataaatgt taacatagtt
     8221 tatggattca tggaatgtta atctaattta atagaaaagg ataattaagt atatacgttt
     8281 tttcttatgt atgttatact atgtatatac agaaagaggt gtctactatg gaaatgttac
     8341 ctataaaaaa atggggaaat agtaatggcc ttagattgcc aaagtatatt atggaatatc
     8401 ttggaattca tacagaagac aaagtgaaga taatacaaga agaagtaaac ggtcagaagc
     8461 ggctaattat cgaagcggct aatatagaaa atgaattaac aattgaacag ttatttgaaa
     8521 attacactga tgaaagaaat catgtgacga ttcaaaattt aggtgaagca gtaggtaatg
     8581 aaaaatggtg agaaaaccta gacaaggcga tattctatta ttgaatacag cacctcgatc
     8641 tggacacgaa cagacaggta aaagacctta tattgtttta agtcatgata ttattgcgga
     8701 ttatagtaac gttgttacag ttgctccaat ttctagtaca actagaaact atccgctata
     8761 tgtaaatata aactcagaat atgaaatgaa gacaacagga aaagtattat tagatcaact
     8821 aacgacaatt gattatgaag ccagagagtg tatttttttg gaaaaagcgc atgatagttt
     8881 agttgaggag ttattgatga aagttagaac agtttttcaa aaagtaagca aataaaatag
     8941 aaacagcagc atgatcaact attcgtgatt gaccatgctg tcttttttta ttctatatac
     9001 ggtttgtctc gtaacattgt agtcttttgc aatcttagcg atcgcaacac ctttttttag
     9061 atcctcaacg atgtttttat aaacaagacg acgttgggga tcttttgcat tggcactgta
     9121 tagcttagga cgtcctttat aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg
     9181 acgtcgttta gattcggtac gttcttgttc agcgatcatg gccaaaattt gaatgatcaa
     9241 atctttaatg aatttatcca ggagaggatt gccaattgct tcggccatga tcggtagact
     9301 tgtgatgatc agctgcacat ttttcttctt caagtaattg actgaagcga tgatctcgtc
     9361 gtaatttctt ccaagtcgat caatagcttc cacaataaaa atatcttgat ctcggacgaa
     9421 atccagtgct tcttgaaaaa caggtcgatg agtaatcgtt gcgccagatt gtttctcaat
     9481 gaaaattctc tctgcaccga attttttcat ttcttcaatc tgccgttgtt cattttgatc
     9541 gatggatgag acacgtatgt atgcaactct caaaattttt acctactttc tgcaactatt
     9601 taaaaaacac cctaatcata cacgagaaaa ccagattttt caatgtcgcg gtcgtgtcta
     9661 gttagggtat accttatata gacaaaaact aatgatggag gaaatagtat gagaattttt
     9721 tgtaagtgtc tactgataac ttattctcaa atgttgtaag gattttattc atttgttaat
     9781 attaaagaac ggaactccaa aaaagtccgt actctaattt gcggtctctc actcttttag
     9841 agtgagagtt tttattagat tttcaaatta attagtaaag tgatagaata aattgatcat
     9901 acaaatcaaa agaagaagca gggtgatagc atacctatgc ttctttttta tgtatttagc
     9961 tatttcttct ttacaatatc gaacatatgt ttgtataatg tg
//
LOCUS       DAPBAB010000082         9964 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPBAB010000082 DAPBAB010000000
VERSION     DAPBAB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.087"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            8..81
                     /locus_tag="RD444_002477"
     tRNA            8..81
                     /locus_tag="RD444_002477"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="RD444_002478"
     tRNA            101..173
                     /locus_tag="RD444_002478"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="RD444_002479"
     tRNA            201..274
                     /locus_tag="RD444_002479"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="RD444_002480"
     tRNA            288..361
                     /locus_tag="RD444_002480"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="RD444_002481"
     tRNA            385..474
                     /locus_tag="RD444_002481"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="RD444_002482"
     tRNA            487..560
                     /locus_tag="RD444_002482"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="RD444_002483"
     tRNA            579..654
                     /locus_tag="RD444_002483"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="RD444_002484"
     tRNA            656..726
                     /locus_tag="RD444_002484"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="RD444_002485"
     tRNA            740..813
                     /locus_tag="RD444_002485"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="RD444_002486"
     tRNA            838..925
                     /locus_tag="RD444_002486"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="RD444_002487"
     tRNA            947..1018
                     /locus_tag="RD444_002487"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="RD444_002488"
     tRNA            1041..1130
                     /locus_tag="RD444_002488"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="RD444_002489"
     tRNA            1143..1216
                     /locus_tag="RD444_002489"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="RD444_002490"
     tRNA            1235..1307
                     /locus_tag="RD444_002490"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="RD444_002491"
     tRNA            1311..1383
                     /locus_tag="RD444_002491"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="RD444_002492"
     tRNA            1388..1458
                     /locus_tag="RD444_002492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="RD444_002493"
     tRNA            1471..1544
                     /locus_tag="RD444_002493"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="RD444_002494"
     tRNA            1556..1627
                     /locus_tag="RD444_002494"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="RD444_002495"
     CDS             1893..3086
                     /locus_tag="RD444_002495"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDT7549821.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="RD444_002496"
     CDS             3216..4688
                     /locus_tag="RD444_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7549822.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="RD444_002497"
     CDS             4771..5559
                     /locus_tag="RD444_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDT7549823.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="RD444_002498"
     CDS             complement(5626..6294)
                     /locus_tag="RD444_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDT7549824.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="RD444_002499"
     CDS             6409..8121
                     /locus_tag="RD444_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549825.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="RD444_002500"
     CDS             8124..9896
                     /locus_tag="RD444_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549826.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DAPBAB010000083         9721 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPBAB010000083 DAPBAB010000000
VERSION     DAPBAB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.088"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(16..912)
                     /locus_tag="RD444_002501"
     CDS             complement(16..912)
                     /locus_tag="RD444_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7549827.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVKDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDPSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(1026..1196)
                     /locus_tag="RD444_002502"
     CDS             complement(1026..1196)
                     /locus_tag="RD444_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549828.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(1193..1870)
                     /locus_tag="RD444_002503"
     CDS             complement(1193..1870)
                     /locus_tag="RD444_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDT7549829.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGNSL"
     gene            complement(1961..2866)
                     /locus_tag="RD444_002504"
     CDS             complement(1961..2866)
                     /locus_tag="RD444_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7549830.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            3041..4495
                     /locus_tag="RD444_002505"
     CDS             3041..4495
                     /locus_tag="RD444_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7549831.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVT
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            4511..5542
                     /locus_tag="RD444_002506"
     CDS             4511..5542
                     /locus_tag="RD444_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7549832.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTQDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENTTGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLDKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            5549..6736
                     /locus_tag="RD444_002507"
     CDS             5549..6736
                     /locus_tag="RD444_002507"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDT7549833.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            6892..8274
                     /locus_tag="RD444_002508"
     CDS             6892..8274
                     /locus_tag="RD444_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDT7549834.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            8246..8632
                     /locus_tag="RD444_002509"
     CDS             8246..8632
                     /locus_tag="RD444_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549835.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(8828..9496)
                     /locus_tag="RD444_002510"
     CDS             complement(8828..9496)
                     /locus_tag="RD444_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDT7549836.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTESWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         2859 a         1933 c         1886 g         3043 t
ORIGIN      
        1 tttttattgc ttttatcaat tcaattttag cgtctcaccg ggagtcagga aataattctc
       61 catgctcatc ccattcagcg acaagattgt ttctactggt acgccagttc gttcggctat
      121 ttgatttgga ccttctcctt ccaaaactac ggttgtcgca gcctgtgaag tcgcgctgtt
      181 ggtattagtt tcagagggat ctgttgtgtc ttgtgttgtt cgagcttgca tttcagaagc
      241 ttctaccgac tctgccgtct cagaatcaga agatgttgtg tcttgtgtgc ttgttgactc
      301 tgcttgaaga gaggcgctag tctgcaaata aatatcggaa atcttagcta catgattcgt
      361 ataattagcc gataattgat tgatcaaagt atctaagtct acaccgtctt tattttctgc
      421 tgcctgatac gctgcacgta attttctgat cccttcgtcg gagattgctt gaagctcacg
      481 tgctttttgg ttagcaactg ctgataaacc agctatgccc tcttgatttc cctgaatttc
      541 tgtctggtat tcttcaatca atgcaggcgt cttttcttcg atctttttcg aataatcttt
      601 tacgattgct tgaagttgct ctggcaaacc atttgctgtt ttttcttcag aagtttgtgt
      661 ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc tctgttttct ttgtcgtggt
      721 ttccgttatg gtcgtacttt tcgcttctga cgaatttttt ccatgaaaat atagacccgc
      781 aactcctcct gctatgatca aaaacacggc aactgctgcc ccaatcatga tcatccgctt
      841 acgagctgcc tgtttttgct gtcttcttcg ggtcatccga acattttctc cccactcatt
      901 ttcatcatac atttttttca ccaccaattt ttatgtataa tagcgttatc aaacgcttca
      961 tattcttatt gtagcaaata attgccgctg tgcacatttt tatctttgtt atttataaga
     1021 aaatttcacc agttgagatg attccaagat tgtccgtcga tttctccttg cagctgcatt
     1081 gttcgttcgt ctatctcagc catcagtttc tttatttcat ataagagcgc acggtcttga
     1141 tattctgatt tttcgcttaa ttcatctaat tgttggatca gccaatcatt tgtcataatg
     1201 aatttccttt catttcttgc agtgtaaagg tcaagtcttc gatctgctct tttgcctcag
     1261 tcagtgcttc tacgatatat gcttgttttt cagattcttc ttcctgcatt ttctgatttt
     1321 cctgtactct ttgaataaac cagtcaatcc gctgagtcag cgatggtttg ccttccaatg
     1381 agagatactg ctgataagca attagcttct cccaacgttt ttcctgggat aaatacataa
     1441 attgtgctaa aacgaatggc ggaagatagg tcatatggaa tttatttgcg attcgctgat
     1501 acggcctcaa aaattcggaa atcgtatatt cttcttttcc tccagcttgg tattcttttt
     1561 ccgccacacc tgttgtcaat acgattccta gttctttccc ttttaataca tgtgctgctc
     1621 gttccaagac attatcttgc caaattttta aatgagcagg agaactatac cagtaaaacg
     1681 gaaactggaa aatgatccga tcatgctggg ccaagagctg tagttcagtc tctatatcta
     1741 tttttccgtc tggataactt gattccaaat gatgccaagt agctggtaaa ccagcagaag
     1801 cttctttgaa aaattgctga tttaaagatt cttcgatcgt tggatggcta atgatgataa
     1861 gtgtacgcat cccttttcct cctttttagt cttcgcttta cagtgtagca taaccgccac
     1921 aagtttcttt tgccgatgct tgtcatttta accagactaa tcaggttgtc caagaaactg
     1981 cggtttgttt ttccaactgt tttttcaagg tatccaaagc aatattctta aaattttcgt
     2041 ttttcatacg tttacgatag ttcgtagccg tttctccggt actctttttg aacattcggc
     2101 taaatacgac tagactattg aaccctactt gataggcaat actttccacg gattcatccg
     2161 tcagagaaag aagaaacgcc cctcgattga tccgtacccc gatcaaatat tccttgatac
     2221 tggtacccat atgatttttg aatactcgcg acaaatggct ccgattgacc gctaaatgct
     2281 gggcgatttc ctcaacagac aatttttcag cataatgtgc ctcgatgtat ttgactgttt
     2341 caacagctaa agaggaatga accttttgta caccaccgct gacctttccg ccatctttta
     2401 aaaggactga taaaaaacgg taggttaatg ctgttaactg taattcattt tccaaggttt
     2461 cttccgtatc tttcatgcat tctaaaataa tgtcgatata tttttgtatg tcagaagaga
     2521 ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat gatttcttct gcagcactcc
     2581 caccaaatga aagccagcaa tacatccacg gatcttctcc atctgataag taaaacgtcg
     2641 agtctcctgg acgaatcaag aacagatcac cttttttcaa ttggaattgt tgatttttca
     2701 catgataaga tcctttgcct tccatcacga catgcaggat gtaatcattg cgaatagcag
     2761 gaccaaaaga atgaaacggc atcgttttag agaaaccaca aaaatcaaaa tacaattctt
     2821 tttctacttg ttgatgttcc ttttttaaaa aaatcgattg ctccacgtct atccctcctt
     2881 actcctttag tataatgcaa caaaaagtta aataaaagtc acagaaatat attaaaaagg
     2941 aagcgatttc ttgttaagtt taagtcatta aaagatcttt gttattcgtt gtaaccggtt
     3001 tacttagtat tatatattga acaggagagg aatacagatt atgaaaagca aaatgatcca
     3061 acgtgtgtcc tacgctttag gcgcttttgg acacgacaca ttctatgtta cattaagcac
     3121 ttattttatg gtattcgtga caagccagat gttcgcagga acagataagg caacgaatgc
     3181 aaaaatgatt gctacagtta caagcttagt tgtaggtatc cgcttagttg aaattatttt
     3241 tgatcctttg attggtggca tcatcgataa tactcgtaca aaatacggta aattcaaacc
     3301 atggttagtt atcggcggcg ttgttagttc cgtactcttg gtaacaatct ttaccaattt
     3361 ctttggctta gctactcata atacgacttt atttatcata ttatttgcaa tcgttttcat
     3421 cttattggat agtttttatt catttaagga tatcgctttt tggtcaatga ttccagcttt
     3481 gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt gctcgtttcg gctcttctct
     3541 aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt actttcttta cctatcttgt
     3601 tactcataaa catattgaag gcgcttcagg ctggttctgg ttcggtgtga ttgctgctgt
     3661 gatttcaggc ggtactgcga ttatcactgc cttgggcaca aaagaagtcg atactgcgat
     3721 ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc aaagcagttg ctcaaaatga
     3781 tcagttgatg tggcttgccc ttgcttatat cgcttatgcg attgccaatg tagcaacaac
     3841 tgccgtatta ttctacttct tcaaatatgt acttggccaa ccaactgaat tttggatcgt
     3901 tggtctagtt gctgccttac ttggtttagt agctgttcca ctattcccaa tgcttacacg
     3961 cgtcatctct cgtcgctacg tttaccttgg agggatcgca atgatgattt cttcttactt
     4021 tttctttacg atcgcaggtt caagtttgcc aatcgttatc atcgggctag tattgttcta
     4081 cttccctcaa caattgattt tcttatccgc cttgatgacg atcacagact ctgttgaata
     4141 tggtcaatgg aaaaatggta tccgtaacga agctgttact ctatcaattc gccctttact
     4201 agataaaatc gctggtgctt tgtctaacgg gatcgtaggg tttgtcgcta tcgcttctgg
     4261 gatgactggt aatgctactg ctgctgatat cactgctcat ggcgtaacga ctttcaaagc
     4321 ttatgcattc tatgctcctg ctgctttgat gattttctcc gctattattt ttgcttggaa
     4381 gattacattg acagagaaaa aacatgcaca aatcgttgag gaattggaaa acaaattggc
     4441 tccagctgat acaatggaag atgaacttca cgatgcagta gttccttccc attaagaaag
     4501 gatcttgaaa atgaagatta cagaaaaaga ttttggccaa ggttatcact tgataacctt
     4561 ggagaacaaa aacaagctag cactttctat ctctgattta ggtgctagaa tcgtcagttt
     4621 aaaatcaaat gatcgagaat tagttttggg atttgatacc gctgaagaat atatcgaaaa
     4681 agatccgtat atcggtgctt ctatcggtcg caccgcaggg agaatcgaaa atggccgctt
     4741 ttctctcaat ggaaaaactt accaattagc cacagatcca aaaactgggc ataatctgca
     4801 tggtggtgct cctggatttg aattaaaaaa atggtcttat gtcattttga atggcgaaaa
     4861 tgaagcttct gtcattttta ctactacaag tcccgacgga gaacatggtt ttccaggtac
     4921 aatggatgtg gaaatccgct atactttgac acaagataat atctggcgag tgacctctcg
     4981 cggcaccagt gaccaagata ctttattcaa tccgacaaat catgtttact tcaacttgac
     5041 gggagatgct tctcaatcaa tcgatcaaca tgagctttgg ctcaatagcg aagcttatgc
     5101 tcctttgcga acagatagta ttcctatagg ggtaaaagaa aatactacag gaagtgcttt
     5161 cgattttcaa atacctaaaa aattagcgtc cgtttttgct agtgatttgg atcaaaaaaa
     5221 tcttgttgac ggaatcgatc atccattttt cttaaaagaa accggcttgg ataaagaagc
     5281 cgctcgcttg actagccccg ataaaagaat ccaagtagat attgctacag atgcttctag
     5341 tgtggttatt ttcactgcaa atttcggcac agaaacacca gaaatgcgca accgaaaact
     5401 ggcacatcat ggtggcatca catttgagac tcaaacagct ccaggtgcag agcgcttctc
     5461 agcatttggt tcgattcatt taaaagccgg atcagtattt gaaacagtga cagaattcaa
     5521 aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa agaagcttaa acacaaaatt
     5581 tgccgaaaca ttcggaccta aaaaaactac acaatacttc tcaccaggac gcatcaattt
     5641 gattggtgaa cacacagatt acaatggagg ccatgtcttc cccgcttcca tcacttatgg
     5701 aacgcaaggt gtcgcagcac ctcgagacga taataaaata ttagtttatt ccacaaactt
     5761 tgacgatatc ggtgtcatta gttttacttt agatgagcta gaatatgata aaaaagcaaa
     5821 ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa gaagctggac actcaatcga
     5881 tcatgggttt gagttgttag tggaaggaac aatccctaat ggtgcaggac tttccagtag
     5941 cgcttcatta gaattattgg taggtgttgt attagaagat ctgtttgatt taaaaacaga
     6001 tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat gaattcatcg gtgtcaattc
     6061 aggtatcatg gatcagtttg ccatcggctt cggtgagcta gatcatgcaa tcttattaga
     6121 tacaaatacg ctgaaatacg aaatggtccc agtgaaacta gatggttatg caatcgtcat
     6181 catgaatacg aataagcggc gggaattggc agattcaaaa tacaatgaac gccgcagcga
     6241 atgcgaagaa gccttggcac gtttgcaaaa aactttgcct atctcttctt taggagactt
     6301 agatgaagaa gaatttgaat caactattga ccaaatcggc gatgatacat tgatacgcag
     6361 agctcgccat gccgtttctg aaaatcagcg gactttgaag gcaaaagctg aattagaagc
     6421 tgggaactta gaagcatttg gtcaactgtt aaatgattct caccactctc ttcgctatga
     6481 ttatgaagta actgggatcg aattagatac actagtagat gctgctcaaa aacaagaagg
     6541 tgtcttaggt gcacgaatga caggagctgg atttggtggc tgtgcgattg ctcttgtcaa
     6601 agaagaaaat atacctgcct tcaaaaacaa tgtctatgat gaatacatga aagttgtcgg
     6661 ctatgctccg gaattttatg ttgcacacat tggaaatggg acaacaaaga tcgatccaga
     6721 agctgttcat gtttgataaa aaaatcaacg atctcagcta aaaacaaata gataaaaata
     6781 acctctcagc aattttcaaa tgtgagcctt ttgctcgtat tcgaaaattg ctgtttttta
     6841 ttgacacaga aagagaacat gtgttctaat tgtattgaag aggtgattgt tatgtgggcg
     6901 aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa gagatatttt atgtattgac
     6961 tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg atcttgatcc ccttacaact
     7021 aaattagtag tcatgtccta tccgtccgat tcgacagaaa cacggggtag cggattaatc
     7081 ttggcttcca gtcctgcagc aaaaaaagct tacggcatca ccaatatcag ccgtgccaga
     7141 gatcttccct tcccctatcc aagtgattta tatattgttg ccccaaggat ggcctattat
     7201 atggaaaaga acagagaaat caattctatc tataaaaaat atgcagacga agcgaaccat
     7261 catgtctatt ccgtcgatga aagcttcctt gatgtaacaa atagcatggc actctttggt
     7321 aaaacaaatg cgtacgacat ggctaaactg atccataaag atgtgtttga gcagaccgga
     7381 atctacacga ctataggaat tggcgataac ccgctacttg ctaaactagc cttagacaat
     7441 ggctcaaaag acggaaaaca aatgtttgct gaatggcgtt atgaagatgt accagaaaag
     7501 ttatggaaaa tctcagatat cactgacttt tggggaatcg gcaaacgaac agcaaaacgt
     7561 ctgaaacaaa tgggtataca ttctgtttat gatttagctc acgcggatta ttatcacttg
     7621 aaaagcaagc taggggtcat cggtactcag ctttatgccc atgcttgggg gatcgatcga
     7681 agttttttgg gacaagaata tacaccaaaa tcaaaaagta tcggaaatag tcaaatccta
     7741 gatagagatt atacaagacg agaagaaatc gagatcgtta tcaaagaaat ggccgatcaa
     7801 gtcggcactc gcttgagacg atctgcagca aaaacacaag tagtcagctt atgggtagga
     7861 tactctttag gctatcttga tccaaatggc aagaccgggt ttcaccagca aatgaaaatc
     7921 gaagcgacaa atgctagcag agatctagca gatgctttat tgaccatttt ccaccagcat
     7981 taccactacc aggatattcg gcgtgtaggc gtgaactgtt ccgacctcgt atacgcgaca
     8041 ggtttacagc ttaacctttt tgaagatcct gaaaagcaga taaatgaagc gaaagttgat
     8101 tttgttgtcg atacgattag aaaaaaatac ggctttcaat caatcatcca tgcgcatagt
     8161 ctcttgcaag gaggacgcgc catcgcacgt agttctctcg ttggcgggca tgcaggtggt
     8221 atgagtggat tagaaggtgg aaaagatgag acggacagca aaaaaattca ttgactataa
     8281 cgagtatcga gatcggccat ttggattaaa atgggggact gcttttgcga tggaagaact
     8341 agtcaacggt atcaaaaaaa atgaagaaga agcacgcaaa cagcctcttc ctaaacaaca
     8401 aatgacattg caagaaatcg atagtatact gacagaagcc tttttatctc gtaaagcagt
     8461 aatcattcag ctcaatttaa aagatgaatt tggaagactc ttggatgagg tcgaagggca
     8521 cttcattggt gaagcctatg aagattactt tatgattgac catcagccga tattgtggga
     8581 agacgtacgg tatattgagt taaaggaaac aaaaaaatgg tttgtgacat gagaggttgt
     8641 gacagaagtg atcaacttca aaaaataaga aaggattttt cgaaaattgc ttcttaaatt
     8701 tttgtggaat tttggcttat ttttcgaaga agcaacttct ggaacaacgt atattagata
     8761 tcagaggttg tgaaaaagtc gcttagtttt gagtaacaat ccaaggcgct gatttcacaa
     8821 cctctgtcta tccatctatt cggtaagatt catgcatgac gtcttcttta tttagtatgt
     8881 agagcccttt ttcttctgac cattcaacat aaatcagctg ttttacatct gcatagcctt
     8941 ctttcttcaa gttctccata agccatgatt cagtcaattg attggactga agcactttat
     9001 gctgaatttg cccttcatct acaagcagga tcgataaagc tttgtcttca tctgattttt
     9061 tcaatacact catttgaccg gcattctcta ggataacatg tttggcattt tcaagagaaa
     9121 agcattcctg ctgtcttaag attgctcgca actgctccat ctcgatatgg ttatttgtca
     9181 actctgtcat gttgatgacg ccgtctttga ttagaacaga tggctcgcct ttgacccaac
     9241 gattgacctt ttcatttttt tggacgatac gttcgatacc ataaatcatt actgcccaaa
     9301 gcgcaatggc aaataggaca tgccctacat ggacattgtc atcataggtt gattcttcta
     9361 agatcccacc taaaacaagc gtatagacta aatcaaacgg tgtgatttct gacatggatt
     9421 tcttcccaag caaacgtacc actaataata aaccgatcaa cccgaatacc agtttcattg
     9481 taattgaaaa aatcattttt tctcccccta actatctgtt aattaaatta tatatgcttt
     9541 ctcacgaaca cgcatggaat atacaaggaa gaaatgtatc cttttactaa aaaaaggact
     9601 gtgacactgt agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt
     9661 ttttcatgct ctaaaatgat atttttacac aaccgttacg gttgttttac gaaattggac
     9721 g
//
LOCUS       DAPBAB010000084         9340 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPBAB010000084 DAPBAB010000000
VERSION     DAPBAB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.089"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            197..1489
                     /locus_tag="RD444_002511"
     CDS             197..1489
                     /locus_tag="RD444_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7549837.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(1701..2543)
                     /locus_tag="RD444_002512"
     CDS             complement(1701..2543)
                     /locus_tag="RD444_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7549838.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(2580..4046)
                     /locus_tag="RD444_002513"
     CDS             complement(2580..4046)
                     /locus_tag="RD444_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7549839.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(4102..5946)
                     /locus_tag="RD444_002514"
     CDS             complement(4102..5946)
                     /locus_tag="RD444_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7549840.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6548..7500
                     /locus_tag="RD444_002515"
                     /pseudo
     CDS             6548..7500
                     /locus_tag="RD444_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(7545..8178)
                     /locus_tag="RD444_002516"
                     /pseudo
     CDS             complement(7545..8178)
                     /locus_tag="RD444_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(8438..8986)
                     /locus_tag="RD444_002517"
     CDS             complement(8438..8986)
                     /locus_tag="RD444_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549841.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(8987..>9340)
                     /locus_tag="RD444_002518"
     CDS             complement(8987..>9340)
                     /locus_tag="RD444_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7549842.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT         2824 a         1904 c         1510 g         3102 t
ORIGIN      
        1 gttttagtga cgataatatg aattttaaga ccataaaaat aagcgttttt cgttgagtta
       61 tagcccctac agctctatac tttttgatgt tggtggatga caccagcatc ttttttattt
      121 tccaaaacat atagaaaaga caccttgaaa tcctttagaa ttaatttggc gaaccaaccc
      181 aaaaggagga atcaagatgt cttacactca ccttatcaaa gaaacattag atattctaga
      241 cttatcagtt acttttaacg aaaattgtct cacaaaagaa aaatacaaag ggcaaatctg
      301 tcatatttat cgtggaaact tgatttatac agcccaagaa tgcatccact gtaaacacca
      361 gattgcttca gatattgtgc gttggggtac aacaaccgtc cggttgttaa tgaacgatgt
      421 ttctgaatgc cgtacctacc tcgaattgaa aaagcaacgc ttcaaatgca aagcttgtca
      481 gcggaccttt gtcgcagata cttctgttgc gaaaaaacac tgtttcatca gtgagaaagt
      541 ccgctggtct gtcgtgactc gtctgaaaaa aaatacctca atgacagaga tcgccgctca
      601 gaaaaatcta tcggtctctt ctgtttaccg tattatgaag cgattttatc gtccgttgaa
      661 tccttttcgg acaccgttac ctaaggtact ttgttttgat gaattcaaat cagttcgggg
      721 tgtttcaggt gctatgagtt tcatcatgat ggatggacaa acacagcgct tgttggacat
      781 tgtcgagaac cgacaactgc cttttttaaa acgttatttt agtcattttt cacgagaaat
      841 tcgtgaagcg gtcgaatgga tcgtgattga tatgtacgct ccttatgtat cgctcgttaa
      901 aaagctgttt ccaaaagcac agctgattat tgaccggttt cacatcgtcc aacatattgg
      961 tcgtaccttt cgcaatcatc gaatcaaaga aacgaaccaa ctattaaaaa gcaaagaaca
     1021 aaaacactat caattaggta agcaattaaa aagatactgg aaacttcttc aaaaagatga
     1081 acgcaagctt gattatactc gacgcctttg gcgacctggc ttcaaagctc atttaacaga
     1141 gaccgatatt gtggatcgct tactgaaagg gagccctgct ttgcgtgtag gctatcagtt
     1201 atatcaagac tttctttacg ctgtaaagga acgagactac gtatcgtttg aagaactact
     1261 taccaataat atcatgcttc ccgaaggcta tcagacgacc ttgagaacgt tccagaaatt
     1321 tctgccacaa atcaaaaatg ctttacaaca aagttattca aatggcccac ttgaatgtct
     1381 gaacaaccat atcaaagtgt taaaacgaaa tgcatatggc tttcgtagct tttataattt
     1441 caaattgcgc atcatgattc gtcatggcaa cgcattgatt ttcaactaaa aaagatcaag
     1501 aggaactgat ccacttgatc tccaattatt ctaagatttc tctttaccaa catcatttga
     1561 caaagagccg agatttttta ttatcttacg ttaatccttt ttaaatgtct cttatagata
     1621 acaaacagct ggaaatcacc ttgtttttac tataatccat ctctaaagat tttaaaagca
     1681 tcaatagtat tactgagcgc tcacccatta gcatgtttca tcaaccgatt gatgtggaca
     1741 cctaggtata caacttcatc gttcggaatt gttttatgat aaactttttc aatgtagtct
     1801 ttgatcttag ctgcgctttc catggcagac gggtataaga tccacatttg attgtagaat
     1861 tcttcctcac catcatccaa caaagcatca gaataatatc tttctacaaa gaaacgtaca
     1921 tgtgtaatga agcgggcata atggatcgaa ttagtatcga tatctttctg caacgaatac
     1981 cggaccaaat tgacgatccc gccgatcatt tttgcatact tgaatccagc gccgctatca
     2041 ggatcttccg attgtgcatt gatcaaatgg aaagcaatgt ttgaggcttc ctctttagga
     2101 agatcaattc catattgctc gttcaaaagg gtgagtgcgt acttcccaat ttgaaattct
     2161 ttgggatagt agatttttat ttcccagtac aaacggtttg caatatttac ccctttttgt
     2221 gcgcgttcaa ctgcaaaatg caggtggtct gttaaggtta aatagactcc aggattcagt
     2281 tttttatcta atcctttttc tgcttcaaaa acgatagtac gagtgatttc aaaaaattca
     2341 ataggtattt cttcaactaa ctctaatagt tgtgaagatt tcttttcttc aaaaggcaga
     2401 aatattttat ctactttatc tcctgatatt tcatccccag ctttctgtcc aaagcctatc
     2461 cctttaccta atgcaatcat ttctttgcca ttttcttcaa ccagtaccac acttgaattc
     2521 aatacttttt ttattgaaat cactcattat ccccctatcg ctgcttaaaa agatcgagac
     2581 tactcgaata aggatgcacc gtttgtttcg atcacttctt tgtaccagtt gaacgaatcc
     2641 tttttcaagc ggttatatgt cccatttcct tcatcgtctg catccacata aataaatcca
     2701 tatcgtttag acatttgaga agttcctgca ctaactaaat caattggtcc ccagctagta
     2761 taagctatca aatctacacc ttcatcgatt gcttctttca tttgttggaa gtgtgcttta
     2821 aagtagtcga tgcggtacgg atcatggatt ttaccatcta ctacctgatc gactgctccc
     2881 ataccatttt ccacaatcat caacggtact tggtaacgat cataaagttc gatcaacgaa
     2941 attttcaaac cttttggatc gatttgccag ccccaatctg ttgaaggcaa atgaggattc
     3001 ttaaccccta aaacagtatt tcccggtgtg cgttctgcgt ttggatcagc tgattctgcc
     3061 atggacatgt aatagctgaa agaaacaaaa tctacggtat gtttctttaa aatttctcga
     3121 tcttcatctt ccattttaat ctgaattccc cgattttcca aatccttttc gattagagca
     3181 ggatattctc ccttaacttg aacatctgca taaaaattat tttctaagtt ctttttcaag
     3241 gtcagctcaa cgtcttctgg tgcgcaagtt aatggataag tcatcaattt cgttaacata
     3301 caaccaactt ggcttcctgg aattttttca tgagcatctt ttgtcactaa cgcactcgca
     3361 acaaattgat gatgcaatgc ttgatagata tcttgagctt cttgaccttc tgcgcttttc
     3421 tcttggataa ttccagctgt tgtaaatgga tgacggtgga tgctgtctat ctcattaaat
     3481 gtcagccaat attttacgta tttgccgaag cgatcaaaac aaacgttagc gaagcgaaca
     3541 aaatcatcaa ttactcgacg ctccacccag ccattatatt taactgctaa agctaatggc
     3601 atttcataat gagatagtgt cacaatcggc tcaattccta aacgttgcat ttctttaaag
     3661 acgttttcat aaaatgcaac ccctttttca ttcggttcca gctcttctcc tgtagggaac
     3721 aagcgagtcc aagcaataga gaggcgcaag gttttaaagc ccatttcggc aaataaagct
     3781 aaatcttctt tatagtgatg ataaaaatca acgccccttc tttttgggta ccatttatca
     3841 gatttgtctt cactagcttc ttttatagac tgcagggtga catgcatatg agcagcaaaa
     3901 tctttgttgt ctaaatttgg tttataagta gccatatccg ctactgaaag acctttaccg
     3961 tctacattcc aagcaccttc tacttgattt gctgctatcg cgccgcccca taaaaatcct
     4021 tttggaaaac ttgttgtgtt tgtcattatt ctattctcct ctattccttc attcaatata
     4081 ttttttttca ctatcttgtg tttaagctat cggactgata gctaagagat cactatcatt
     4141 ggttaccaat ttttctaggt taaatgaacg gatagtgtaa ttatccttat tggttaccac
     4201 acagataact gtggggtcga atcctgcttc tttgattaaa tcaatatcaa atgcaatcag
     4261 cttttgtcct ttggctactt tttcaccaga acgaacaaat gcctcaaacc cttttccttc
     4321 taactgaaca gtatcaatac caatatgaaa aagaagttct gcaccattag ccgtttttaa
     4381 accaatagca tgcttcgttt caaataccat tgtgatttct ccatcttcag gagctactaa
     4441 tatcccttct tcaggaatga ttgctactcc atcacctacc ataccactag aaaaaacagt
     4501 gtcttttact tctgatagtg agattacttt tccattcacg ggacttgaaa gaacagcatc
     4561 tgaaacaacg gccgtttcca tcttagtttc aacttcattg acaacttctt tatctttata
     4621 aagaaccaac gctgcagcaa aagcaaccac aaatgaaatc cccgatactg caaatgccca
     4681 aaccagattc attgggtcag ctttgtcagc gaacatcgtg atacttgcaa tcccaggacc
     4741 cactaaagca aaggcttgaa gtgcaacgat tcctgcaaat gctcccccga tcaggctaga
     4801 aaacataacg gcatataaaa cacgtttgtg cagaatcgtt accccgtaaa gagctggttc
     4861 agtaattccg aataatgcag atatacccgc agaaacagcc gtagatttca attttttgtc
     4921 ttttgttttg atactgacag caaagcatgc gccagcttct gaaatattgt gtgccaatga
     4981 agccgggaga taaagaagtt ctgcacccaa ttcactcatg gatgaaacag cgtaagggat
     5041 cattgcttta tgcatacccg ttacaaccat taatggcagt acagctgcta agactccggt
     5101 tgcaacccat ccaaattgat tgtacaaaga cacaatgata gaagagaaag ctgttcctac
     5161 attataacct aatggtccta gaagcaacaa agcaatagga actgtaatca ataagctcat
     5221 cataggtaca aagaaaatac gaattggttt tggtgaaact tttgtaaacc atttttcaag
     5281 ttgagcataa aataaaacag ttaagatggc tgggaatact tgagatgcat acgctatatt
     5341 atccaatcca aaagataaga atgtagcacc ttccgccaac atagctgtca tttcaggaag
     5401 gatcaatgca ccgacagcag atactgcaac caattgattg actttcaatt tattagccgt
     5461 agtcattgcc accagaatag gcaagaaata aagcggtgcc ccgccgacca aatttaaaat
     5521 ctgataggtt tgagattggt cactaatcag ccctaagata gaagctagta gcaacaagga
     5581 cttcaatact cctccacctg caatcgcagg aattaaaggt tggaaaatag aaataataaa
     5641 atctaataga actgaaccta atttttgttt ttgttttggt gcttcttcag tatttgaaac
     5701 attgtcacct aataacgctt tcacttcatc atacacttca acaacatcgt ttccaatgat
     5761 tacttggcat tgcacatttt ggcgaacgcc cataacgcca tccacttttt ctaactggct
     5821 catatttacc ttgctgttgt cttttaacgt aaaacgtaaa cgagttgagc aatgctccaa
     5881 atgggatatg ttttcttccc caccgatgta ctgcaatact tcttctgctg tttgtttgta
     5941 attcatggtg tttccctctt ttcttttttg agtgatgaac ttttgagtcg aagtggccat
     6001 cctcaacaca aaaaacaggc agaaactaaa taaattgaca acaaggcaaa cgtgtattga
     6061 aacgcataca ttgttattca acttatttag gtcctgcctg cattaccagt cacatgcctg
     6121 tttaagttat attcaattta acatattaga atgtaaacgt aaataataaa atgatttttt
     6181 aatcattttt tcattttatt cgttcaatat agttttcgtg ttctaaaagt tttgtctttt
     6241 ttctaagaat atcttccaac atatataact cttattctct tctatcttct agcagatgtt
     6301 ctgtcttttt aagacctagt ataaaaagaa tgggttcatt ttttctttct tgcaggatga
     6361 ggttttttat ataaacttta tttgatataa agaaaaggca ttcataattg tgctatacta
     6421 ctaaaaaatg ataatacagt gaattagatt agcgctttat taaattaaga ggacagcata
     6481 tcgatattgc ttcctttttt ttgctattta tttaagggat agaagtttag aaaggactgt
     6541 ttataagatg aaaaatgata tagtgactta tgcaaacaca aaatgtgaag aaattggcat
     6601 cagtaattta gaagagtgct tttttgacac aaatgacctg tcgcaatttt taagaacggg
     6661 aagaacaaat gccagtaaac ttgccaatga cttagttgca aagaacgttc tatttaaaat
     6721 caatactcgt ccagtgttat ttgccatcaa agagcgcttt gaagagcact ttttgattcc
     6781 ggtaaaagat gtatacgaaa gtatatctga attaaaaaca gacttggagc ccaattctac
     6841 agattccatt ctacaaaaga tgattgggta tgatatgagc ttatccgatg cggtggaaca
     6901 aatgaaaact gctgtcttct atcctggaag agggcttcct atgatgatca ctggagatac
     6961 tggagttgga aaaagttttt ttgttcatac tgcttatcag tttatgaaaa aagtgaacgt
     7021 tattcatgag aatgctcctt ttaaagtact taattgtgcg caataccata ataatcctga
     7081 attattgtcg gggatactat ttggctatgt taaaggtgct tttaccggcg cagatgaaga
     7141 tcgaaatgga ttgttgcaag atgcagacgg tggagtactg tttttagacg aagtacacag
     7201 attgcctcct gaagggcaag agaaactatt tgtttatatg gagatggcgg aacttattct
     7261 agagtcggtg aaactatcca tcacgcctcc aatgtacgtt tagtttttgc gactacagaa
     7321 ggcaaagagg attttctgga aacctttatg cgaagaatcc ctatacatat ccatatccca
     7381 aatattgagg aacgaggtat tttagaaaaa aaacaaatta tcgaaaatac tctctttagc
     7441 gaaagcaaaa actttagcaa ggctattgat gtttctccta aagtcactag tgttgcataa
     7501 atatagtcag aatattctga attattattt tcttgaaaaa agtaaaaaaa atcgtatccc
     7561 aaactttcat ctacatttcc agaaagatct tcatgttgat aatcaattcc tgaatcaatg
     7621 accgctacct tcacatcctt agaacccttg gtttcttccc aagcggatgg catgctaatc
     7681 tctcttaaat tagcttgttt actccaatta ggatcgttat cgatttccta aagaatcagt
     7741 tgtatttggc tcctcaacat cattcaacgg ctctagttca accacatagt ttggctctgc
     7801 ataattgacc atatcagaag cattaaatgt tcttactgcc tcttccacac taatttcttc
     7861 gttcaatttc actaataata cttgatctcg cttattataa tcgctttcat ttgtcctaag
     7921 agtcaaatct ttttccacca taacttttac acccaataaa ttttgcggtt tatttttctc
     7981 tgttgaaatt ctttgtgtag cctttaaact gacaattagc tcattgggta catactctat
     8041 ttgagagtca tctaattggc tcataatcct ttctttttgg ttagtatctg cagttacttg
     8101 tttagtatga gccatgaacc caaacatact taccccaaca accaatccta aatatatttt
     8161 ctttttaaac tttttcatct acatctcctc ctaatagttt tattttagag ttagctctat
     8221 tacttattta ctaactcaac aacagattat catacacttg ttaatgaaat cacaatatct
     8281 cattaaaaca caccctaaaa tcataaccgt tcctatgctc attttttgga ttttttaatc
     8341 acgtaaccag aaaaataaat cccctctatt tcacgcaaat caaagaagaa ctaagtaact
     8401 accgtttaaa ggtagttact tagttcttct ttgattatta tctaacttta ttattcattc
     8461 cccacccttt gcgcagtgag tcttcggtat cttgttttat ctgactggta gttcttatta
     8521 tttctccacg atcatctaat tctggaacaa ttggatattt ttctctgaac aagtctagtt
     8581 ctttcaccac gtcattaaac aaatcttgtt tatcttcttg cgtaagattc tgtttaatat
     8641 actcatctaa agcttttcta agcaattcat ctcttgtcat attagtctca tacttttcta
     8701 ctaaaactct ggaagtcgca tttaacaaag tcgacagttt taaaggatgc ttggtggaat
     8761 aaaccttatc aaactttctg attggtgcgc caactttttt tgttcttcct ctactctgtc
     8821 tttcaacatt ggaggaagtt tttatttctt gaacttggtg cacttctaat tgcttatttt
     8881 cagatggtgt aattgcttgc tgctttttct ttgcttctgc ttcttttttt attttggcga
     8941 gtcctttgtt gttcccaaaa atatcaagtc ctttgatgtc agccaattac tccacctccg
     9001 cataccaatt ctcgtgttct tccaattctt tcaaaatact gataaataat tcgtggcttt
     9061 tgcgatccca ttgttttaag agacctttct ttgtcttctc catcgtaata cctgatccgt
     9121 cgtatcgttt taagcgcgca tttttgagta caacattttt aatcaaatgt tccccataaa
     9181 tctcttgggc ttgtttatac atttcctgat ctactgcgtc accgctttcc aacatgaaag
     9241 gaataacacc agcaacatat aaatttgtat caaattcttt cacaaaaaag tccatatact
     9301 caagatattg catcgcattt ctcatcgatt ttacttgggt
//
LOCUS       DAPBAB010000085         9327 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPBAB010000085 DAPBAB010000000
VERSION     DAPBAB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.090"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            316..1101
                     /locus_tag="RD444_002519"
     CDS             316..1101
                     /locus_tag="RD444_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7549843.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1114..1965
                     /locus_tag="RD444_002520"
     CDS             1114..1965
                     /locus_tag="RD444_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDT7549844.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2089..2541)
                     /locus_tag="RD444_002521"
     CDS             complement(2089..2541)
                     /locus_tag="RD444_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549845.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3006..3374)
                     /locus_tag="RD444_002522"
     CDS             complement(3006..3374)
                     /locus_tag="RD444_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDT7549846.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3524..4006
                     /locus_tag="RD444_002523"
     CDS             3524..4006
                     /locus_tag="RD444_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDT7549847.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4043..5053
                     /locus_tag="RD444_002524"
     CDS             4043..5053
                     /locus_tag="RD444_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDT7549848.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5168..5881)
                     /locus_tag="RD444_002525"
     CDS             complement(5168..5881)
                     /locus_tag="RD444_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549849.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(6071..6661)
                     /locus_tag="RD444_002526"
     CDS             complement(6071..6661)
                     /locus_tag="RD444_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDT7549850.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7184..7855
                     /locus_tag="RD444_002527"
     CDS             7184..7855
                     /locus_tag="RD444_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7549851.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            7852..8013
                     /locus_tag="RD444_002528"
     CDS             7852..8013
                     /locus_tag="RD444_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549852.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            8497..9267
                     /locus_tag="RD444_002529"
     CDS             8497..9267
                     /locus_tag="RD444_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDT7549853.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
BASE COUNT         3209 a         1445 c         1762 g         2911 t
ORIGIN      
        1 gctgtgacaa aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc
       61 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gtaatttttg taaattactt
      121 cttatttctt gaagctggcc acttctgtca caacctcttt ctttatagaa ataaaaggac
      181 actagcaatt catgaaaacg attgctagag cgtatcattt gctttagact atatatcttg
      241 gtgtacactc actttaagac ggaaataaaa gttatccgta ataacaggtt taggtatgaa
      301 aaaggagtgg gtactatgta taaggaatta gaaaacaaag tcgcagtagt aactggtggc
      361 tcaaagggga ttggaacagc gattgctcaa cgattcggga aagaaaaaat gaatgtagtt
      421 gtcaactata actcggataa agaaggagca gaaaaagcag tcaaagcaat cgaagaggca
      481 ggcggaaaag cagtctctgt acaagcagat gtcagttctg aagaaggaat acagcgttta
      541 cttgatacgg ctttagaaaa ttttgggaca ctggacgttt ggattaataa tgctgggatg
      601 gaaaatcagc atccaaccca tgaacttagc cttgaagatt gggaaagagt attaaaagtc
      661 aatttgactg gcgtttttat cggaacgaaa gcggctttaa attactttac agagaatgat
      721 aaaaaaggaa atatcatcaa tatgtcttct gttcatgaac aaatcccatg gccaacgttt
      781 gctcattacg ctgcttcaaa aggcggagta aaactattca ccgaaacggt agcaatggag
      841 tacgcaccaa agggaatcag aatcaatagt ataggacctg gtgctatccg tacacctatt
      901 aatgcggcaa aatttgatga tcctgaacaa aaagaactct tggaaagtat gatcccacta
      961 ggacgcgtag gtgaaccaaa agaaatagcg gcgacagctg catggttggc ttcagacgaa
     1021 tccagttatg ttacaggtat tacactattt gcagatggtg ggatgacttt atatccttct
     1081 ttccaaggtg gaaaaggata agggtgaata aaaatgggtt tattgattgc attgatcccg
     1141 gcagttgggt gggggatcca gccactgatt ttgaaaaaga ttggtggcag accaacaaat
     1201 gagatattag gaactggtat tggagccgtg ctcgttggct tacttgttca attattcgct
     1261 tctccagctg gtatttcagt gactacattt ttgattagtt tactttctgg tgcattctgg
     1321 gtcatcggac aaacaggaca gtatacatct ttcaacttga ttggtgtttc taaaacgatg
     1381 ccaatttcga ctgcattaca gttagtcgga acctcattga ttggtgtctt cgcttttgga
     1441 gaatggtctg gaagtatggg aaaaatcatt ggtgcagcag caatcattct tcttgtattt
     1501 ggttctgctt taacggcaat cagtgatgat cctggaaaaa aaggcggtat cacaaaagga
     1561 atcagtctat tagcatcaac aagtattggt tattgggttt acagtgcatt gcccaaacta
     1621 gttgatgcaa gcggactttc cattttcttt ccgcaaatga tcggtgtctt tttaggggca
     1681 gtgatttatg tgattttccg aaaaccaacc gcattttctg aagacaagag ttggaaagca
     1741 acaatagttg gagccatctt cagtgtgtca gcattggctt atattttttc agcaaatgaa
     1801 aatggggttg ctactgcata tatcattaca cagctaaacg tagttatctc tacattaggc
     1861 ggtatgtaca tcctgcatga aaataagaca ccaaaagaaa aaaaattaac gattatcgga
     1921 ttattgctga tcgtagtagg aagcatggtc acggtcttta tttgaggata aagatggtaa
     1981 aatctcattt attaaaaaaa tatttgtttg aaccaaacag taagtactta taaaaaaata
     2041 gcgctagaat ttccaacatc tctggaattt ctagcgcttt ttaattgttt aagatacaag
     2101 tgaaatatct gtctgcatca ataatttttc attgatccat tgactaaagt ttacgacata
     2161 attgaagtta ccaccgatta gtatctccgc atgattgtct ttataaagat cgtattgtaa
     2221 aagacggtct tttttatttg cgtaaacatc gttatggtac tggactaatt gttctcggaa
     2281 ttgattgatt gctgtaaaat cacgatactg aaggttgaaa cgcatcgtga ttggtgcatt
     2341 tagcatgaat cgctgcattt cttttgctgc ttcctctgct tgatctccgt ataggaactc
     2401 aaaaagatta cgttcagaaa atttccattg cccgccaacc tttgttcctt ttagttttcc
     2461 ttcatgaaga taatttctga tagaacgtgt agtaacacct aattgttcgg ctaattcatt
     2521 tactgagtaa aaatctttca atgttcaccc tcctatgtga atggttctac tgacttagtt
     2581 gatatttgct taatcacgaa atgtagaaag aacgatttta attaaggtca ttgtaaaaat
     2641 caagtcagaa aattctaact tttctttctc taaagaaatt cgttctctcc taaagtgata
     2701 ttatatttaa atatacctga aaataatggg cagtattatg aaagatcact gatattttct
     2761 cgtatttttt ccttattcaa aagttttgca ggaaaattgc tgaaaaaata attttactaa
     2821 gtggaaaatt caaagccatt caatcagtga tttttttcag cacaagaaag actccttata
     2881 aaaatagcag atactttgtc aattaagtgt ttaaccaggg atgataaaaa ggataggatt
     2941 gaacaaaacc tgtataagct gatgaatagt ttcctcgcca gtaaaaggtt tattcgtata
     3001 aagctttatc gccaaaattt atcatgacct tttttatctt caatattaaa tgaaatcaat
     3061 tgttcaatca attcccagtt gattacactt ttccaaggaa ctttgatgac gttatctgta
     3121 tatacgtagc ctgcttcttg gatatttttt tcaagggcac gaatcgctgc tttttccggt
     3181 gcgatagaaa aatgattttt agcaaaacta aatgcaataa taaaggtacc atggtcagta
     3241 aacataggtt ggttccattt tacagttgtg tctagctggg gatacgtttc tgttatctta
     3301 gcaaaaaggt ctttgatctt ttgttgatgt tctttttctt tgatctgatt gatgatcggc
     3361 tgaaattttt ccattctaaa acgcctccat taagtggttt gatttatttt accagaaaaa
     3421 caatagttat gtataaaaaa cgaacaaaac acaagtaatg ttgtaaccgc tatatttata
     3481 tgataagatg tctcaaataa taaaatagaa aagagacata aaaatgattg acttggcaaa
     3541 gaaattctta cacgaaaata tatattttga gttgtatcat catcgagcca tttatacaaa
     3601 tgaagatgct ttggtaataa agaaagaaca aggatttaca ggtacagaga ccaaaagctt
     3661 attcttaaaa gataaaaaag ataataacta cgtatatctg acttttacta cgaaacaaac
     3721 ggatttcaaa gcattgggga agatcgtcgg taaaaggctt tctattgttt catcagaaca
     3781 catggaagaa caaacgggac aaaagcaggg cgctgtttct ccgtttggtt atgaaaaaca
     3841 gattccgatt attattgatg aagagctctt attaaatgaa aaacttgttt ttgcaccagg
     3901 tagaccagat caaacgatgg tcatccaaac ggttgactta aacaaaatca tccagctact
     3961 tgagatgccg atatttttga tgccggcaaa cgaaaaaaac aattaaccaa gtaaaatagt
     4021 gcatacttgg aggtgaagca acatgataag ttatccatta ttagaaagaa atacaacgat
     4081 tggggttact gcaccatcct caggcgtatc agaggaacac cattcgttac tcgaatcagc
     4141 tttaaagaga atggaagaaa aatctttcca agtagttact ggaaagacgc tttggacgca
     4201 agataaggaa aaatcaggtc cagcaagaaa acgagcagat gaattcaatc aaatgatgca
     4261 agatgaaaat atccaaatga tctttccgcc ttggggagga gaattattga ttgagattct
     4321 cgattttgtc gattttgatg ctctacaacc aaaatggata gtaggttatt cagatattag
     4381 tacgttgcta cttgctgtca cattaaaaac agggatagcc actgcacatg gaatcaacct
     4441 tattgattta agaggagagt acttggatga aacgacagca atgtgggaaa gtgtactgaa
     4501 aacaagagaa ggagaatcga ttctacagca accttctgaa aagtatcaaa aagagtggcc
     4561 ccatgagcat tcatcatctt ctgttttcca tctcactgaa ccaacaaatt ggcaatcagt
     4621 cagcggagat gcagtaagga tgaagggacg attattaggt ggctgtattg atacgatcag
     4681 tcatttaatc ggtacccctt atggagatgt ccaatctttt aaaagaaagt ataatgatgg
     4741 aaagccgatc ttgtggtttc tagaaaattg tgagctatcg tcagttgatt taagaagatc
     4801 acttgttcag atgaaactgg caggatggtt tgatggcagc tcggggatca tgttcggtag
     4861 aagtgcagca gaggatttgt tagattataa aatgaaggat gtatacgaag atctgtcaaa
     4921 ggaattaaaa ctaccgatct tgtatgatat tgattgtggt catgtaccgc cacaactgac
     4981 attgatcaat ggtgcctatg cagaagtagc ggtagaagat agaaaagcga ctgttttgca
     5041 aacgtttatt taaaaaattt tttcctgcct tggagtttat caaagaccaa caaatctaaa
     5101 tagaaaagaa aatgaaagaa gctgacagta ttttgccagc ttctttttgt atgatctatt
     5161 agaaatttta attatccata actatcacat tgatcagctg tttcacatca tttggattac
     5221 ctgtaggaat agctactaca gatgcattat caataatttc ttgagttgtt tctctaatga
     5281 acgctggata agatgtttga tctgcaggca tttgccaatg tgtaggaact ggataactag
     5341 tgattgtccc gtcatggtta cttgaataca ctaaaactcc ctggtaactg tatttcccag
     5401 tgagcataac cacatctttc ggatagactg cactccctgc atcataaggg gcgataggtg
     5461 ttcctgcagg ataatgaata acatgtagtt cgaaatcact cgatgtatct ctaaaagtat
     5521 tacctctaat agctagccaa acacgtgcgt actcaatttg tagatcagaa tatcctgcta
     5581 acggatctat tgactgagaa gactgtgagg aagttgtgtc tattgttgaa gaagaaatat
     5641 ttgtctcaac tgtactacta gatgtagaag aagtattggg ctctgagact ttactgcttg
     5701 ttgaagatgt attcgtttca gtagaagctg atttctgagt acttgatttt tcagctgaac
     5761 tggtttgtct gattgaactt ttgtctgtgg tcgcagatgg atcactattt tctttactag
     5821 gagacgtaca tcctacaagg aataaagaga ccaacaataa aataaaacct aactttttca
     5881 tataaaatac ctcattattt ttttaatacc agtatagcgt aatcctgaat ttttgaatcc
     5941 gtaaaacctt gtctatgaat ataaatagag tatatcttct ttgataaatg tcagaaaaag
     6001 tgtgacaaat aaccatcagt aaaattactt gtcatactat tgagaaggag tatttaattg
     6061 aagagaatga ttaggagaaa ggatctccgt actttttata gtaatcatag aatccaagtt
     6121 tgtatagacg aatcgttttt tctcttattt cttctgtttc aaaatggact gcttgaagaa
     6181 tcaagtctgt gaattctata ggagctgttc cattcgcagt aatcaaatta cgatctttca
     6241 cagcttgttg ttctataaaa tcttctttat tctgataatt ttccgattct aaccaaggaa
     6301 attgcgcatt tccggtgtgt ttgtaacctt gcaataatcc gctctgagct aaataatcaa
     6361 ctgccccaca aatagcagct actggtacgt ctgtttttaa caatttatcg attatttgat
     6421 ataaatgatg attttttatg tcccatgaat tgccaccgat cataataaaa aggtcaactt
     6481 tttcaggtat ttgttcgatt aagtaatcga tttttgtttt taatcctcca atggaagtga
     6541 cctctgattg gatagaagca gttttgtttt gccatccctc tttttggttt agtgtgctag
     6601 taatataagc accttcccaa tcggcatatt cctctaatag aaacgtaatc gcagttttca
     6661 ttttcaagct ctcctttact atctatgaca aggacaacag ttttaacata cagacattca
     6721 ttaaatttgg atagcgacag cgaaaacaac aattcttaag ttttagacag agtatacaga
     6781 cagtcaactt aattaaatac gaactaaata aaaatgacag gtaatcattt ttgtttacca
     6841 gcattataac attttgcata catggatgac aaaagaacca attcccactt gcttgagtac
     6901 aatcttaaaa gaaactggaa ggcaaaaagt aattatagaa acgaaaaata aatcatatcg
     6961 ggcactcaga catgaaaaca accctagaaa tatataacta gtccaatgaa aaaacagcga
     7021 atgccgtaga aaacaaggat tctgcgtaca ttcatatctc attttgcgag catatctgct
     7081 attactgtga ctaaatgtta atatggttaa aatgaccgtt atatttttta tataaatatc
     7141 gtataattag tatgaatata gaagaaggaa agaaggtgca atcttggaac ctaaagacaa
     7201 gtatttagat tacgctaggg tcaataaaga taaatttatt gaaacaatca tcaagggaaa
     7261 aatccctgat gatgaaaaag atgcggtatt catggctgga agcccaggag caggtaaaac
     7321 agaggttgca ctaggtttag ctgaaaatta tgacaaccat gttgtgattg atgcggacta
     7381 ttttagagca caatttccag aatacaatgg aaagaactca agtgtttttc agaaagcttc
     7441 ttcttggtta gttgaacaat cattaaaata tgtactggaa catggctatt ctttcatact
     7501 agaggatact tttgctatcc ttagtgcaga gaaaaatatt attcgagccc ttaaaaataa
     7561 ctttcgcgtt acgattttct atgtttacca agatcccaaa gttgcctggg atttcactcg
     7621 aaaacgtgaa ctagctgaag gacgtcatgt gccaaaagaa atgtttatca atgcgttttt
     7681 taagtccaga gagaatattg aaaaagtcaa aaatagacat cctgaagtag tccttcacat
     7741 aatgattaag gattatcaaa acgacatttc tgaagtgcat tatgcagcag ataatattca
     7801 attagtacta cccaatacaa tatacttcaa aagagttgga ggaggaatta catgactgag
     7861 aaagaacaag tacaacaaat cgtgaagaag tataataaaa gtatcgctga tttatcagaa
     7921 aacgcctcag cgaaagaatt taaaacagta atgaagtacg tagcagatga agcaaatcgt
     7981 aagcagcgaa aactagttgg gttggataaa taaatttttt aaaccatatc cttgtaaagt
     8041 tgagtttttt gttttaatga attaagacta ttaaatagat aattattgta acttaaaaaa
     8101 cacagctaaa aattagttgt gttttttatt aggtttactt ttctggtata aaaggaacta
     8161 gctacttttt caaaatttct aaatcattca atatgtagcg cttactaaat tattcgccat
     8221 aaaattttat aattggatca ctcattgttt tacctaagtc atatttaagt aataaccggt
     8281 gtttttacat aagaagactt ggggtatttt ttagtttgat tagctctgat atacctataa
     8341 agaatatgct aaggtaagaa tagtaaaaat tcacatagat gaggtataat aatgatgttt
     8401 ttatatatta cagataactt gacacaaaat gagttttttt cgatttttca agttatctaa
     8461 tgcaattata ttgatatcat taggagctgc attgttatga atccaacatt gattactaag
     8521 aaagaattac taaaaaaatt agatatttct actggtgtat tagctgattt aattagaaat
     8581 ggcatgccaa aagagggaga aatgtttaat cttgataaga ttattacatg gcgagaaaat
     8641 tggtctaaaa acatattagg tgagttagaa gttggtagag tatatactaa caaagaaatt
     8701 tcagagaagt ttaaatgttt caaacagggt ggaatgagaa gatctcatca aactaatact
     8761 cttgtactgt ttagtgatca aacaggaagt aatgtttata aagataagtg gttaaatggt
     8821 attttacaat ataccggtat ggggctaaaa ggggatcaag tattggataa gaatcaaaat
     8881 aaagttttag ccaattcaaa aagtaatttt gtaaagattc atttatttga aacatttaaa
     8941 ccaaaagaac acacgtatct gggagaagta tatttagcag gacaaatata tactgttaat
     9001 gaaaaagata gttcgggcaa cagcagaaaa gtatataaat ttccattagc tttaataaat
     9061 caagagcaat tgattgaaga taaagatata tataaccagg aagaaaatca aactagacat
     9121 attcgtaatt tatctgatgc taaattagag gaagaagcaa gaaaagttag taattataat
     9181 aaaaaaatgg ctgcaaaata tgttaacgaa gaggagtttt gcttctcaaa aacaaaaata
     9241 tataaacgta cccgtggtgc tagttaattt taatatccta aagaatatct caaagttcct
     9301 tcaaaacgtc gttgtgctcg acttaat
//
LOCUS       DAPBAB010000086         9278 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPBAB010000086 DAPBAB010000000
VERSION     DAPBAB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.091"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(30..587)
                     /locus_tag="RD444_002530"
     CDS             complement(30..587)
                     /locus_tag="RD444_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549854.1"
                     /translation="MLLDDKVEFKKISNIDDKERALVEAFSSGKDHIDSYLKNEALED
                     LQYGITKTYLMFVTPKGKETYLLGFFSLTTDRVLITKKSKLQDLLTNWKNPVYRKSIP
                     GIQIHHFAVNGPYQEQHLGQEMMYYAFLFIKTALLSNIGACLITVQSEPDVRGFYESL
                     GFCHTGQVRDSNVSMAIPTNEFFID"
     gene            complement(600..779)
                     /locus_tag="RD444_002531"
     CDS             complement(600..779)
                     /locus_tag="RD444_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549855.1"
                     /translation="MATTIKNTELVFNSEKECSKFLDDIYKNKKVPSNSKIANTLAGM
                     RKIKTINVDGVKYEV"
     gene            1031..1104
                     /locus_tag="RD444_002532"
     tRNA            1031..1104
                     /locus_tag="RD444_002532"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1065..1067,aa:Ile,seq:cat)
     gene            2301..4748
                     /locus_tag="RD444_002533"
     CDS             2301..4748
                     /locus_tag="RD444_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7549856.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            4751..6934
                     /locus_tag="RD444_002534"
     CDS             4751..6934
                     /locus_tag="RD444_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7549857.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            6931..7932
                     /locus_tag="RD444_002535"
     CDS             6931..7932
                     /locus_tag="RD444_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDT7549858.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            7947..8861
                     /locus_tag="RD444_002536"
     CDS             7947..8861
                     /locus_tag="RD444_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7549859.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            9106..9222
                     /locus_tag="RD444_002537"
     CDS             9106..9222
                     /locus_tag="RD444_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDT7549860.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2979 a         1681 c         1829 g         2789 t
ORIGIN      
        1 gaggggcggt acatattatg taacttttcc taatcaataa aaaattcatt agtgggtata
       61 gccatactaa catttgaatc tcttacttga cctgtgtggc agaaaccaag agattcataa
      121 aaaccacgga catcaggttc gctttgaact gtaatcaaac aagctcctat attacttagt
      181 aacgcagttt ttataaataa aaaggcataa tacatcatct cttgacctaa atgttgctct
      241 tgataaggtc cgttaacggc aaagtggtgt atttgtatac cagggattga tttacggtaa
      301 actgggtttt tccaattagt taatagatct tgtaatttgg atttttttgt aatcaataca
      361 cgatcagtag ttaaagagaa aaaacctagt aagtaggttt ctttaccttt tggggttaca
      421 aacattaaat aagtctttgt aatcccatat tgtaaatctt ctagtgcttc gttttttaag
      481 taagaatcaa tatgatcttt tccagaagaa aaagcctcta ctaaggctct ttctttgtca
      541 tcgatatttg atattttttt aaattcaact ttatcatcaa gtaacatatc tacctcgttc
      601 tatacctcgt actttacacc atcaacattt attgttttta ttttcctcat gccagctaat
      661 gtattagcaa tttttgagtt tgaaggaact tttttgtttt tatatatatc gtctagaaat
      721 ttgctacact ctttttctga attgaaaact aattcagtgt ttttaatagt ggtagccata
      781 aaatcactcc tcactatgtt gattttttat gtacatatag tataccaact tagaatctta
      841 gagtaaacat aaatcacttg acaattttaa atatgtttat tagcatattt tgacttctaa
      901 catgctgaat attctagttt ttaatttgaa actgtgtaat aataaatatg ccatcacaac
      961 aaagaatgaa acccattatt atctagtcta tctccattct ttttgttagc aggagttgtg
     1021 gtggctttcc gtacccttag ctcagttggt cagagcaaac ggctcataac cgtccggtcg
     1081 taggttcgag tcctaaaggg taccttaatg tagccatttg aatcgttatg tgttagaatt
     1141 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1201 ttttatgtat tcttttatgg attaatgaaa ggatgtttca catagttata cttctgtata
     1261 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1321 taataaagaa gagtttaaag cgttccccaa aaccactccc ccataagtgt gttacgcttt
     1381 aaactctttt atatttgaag ccattaaaag cataccatat aactgtaaaa aataatggaa
     1441 aaaagactta taattggagt gatagttaat tagtgactta tttttgattt tatagcactg
     1501 atactataaa atatagatat catcatatta cacaatctta ataccaactt aaaaatatct
     1561 cctttcataa gtatggtgat aaaatccgtt ccgggctacc tttttaggta gcctacttta
     1621 atcttcaaac aaaaaatata catctttctt cattctagtt ttcgcagcag gaattttaaa
     1681 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa
     1741 aacttctgaa tcccatactt tataaaaatc tttgacaaat ctattcgaag gatcaaattt
     1801 aaaccataat tgtgtctttc cttctattaa aatatcttag ttatacgcat cttctttgaa
     1861 atttaaggta attgatatgt atcccgacca aatattaggg ttgttagaaa ttaaagacac
     1921 tagtcctagt gataataaaa taccagataa ttatacagcg caacagtaca ttgaagatat
     1981 ttacaatgtt agatctgaaa tacaaagagt tctagataga attgaaaatg aaattggtaa
     2041 gataatgatg taaatggaaa gatcgtgata aagtttttgt tactattttt aataatgcag
     2101 caaaaaaata ttgccaccat tttgccaccg atgactgagg ttttctcagg tgttttgaaa
     2161 tgacctaaag atagaagaga ttatttagtt cctttataac ctttaatact aaaagcttag
     2221 agactatctt agattaatag agaatagtac agttagatta ttgcgaaaag aagccattac
     2281 agcagttagg agtgaatttt atggcatatc caattaaata tattgaaaac aatctggtct
     2341 ggaataaaga tggggaatgt tacgcttact atgagcttgt tccctacaat tactcatttc
     2401 taagtccgga acagaaaata caagtacatg attctttcag acagcttatc gcacaaaatc
     2461 gtgatggcaa gattcatgct ttacaaatca gtacagaatc cagcatactt tctgcacaag
     2521 aacgttccaa aaatgaagtc actggaaagc tcaaaacggt tgcctatgac aaaatcgacc
     2581 aacagacaga cgctttaata tccatgattg gcgaaaatca agtggactac cgtttcttta
     2641 tcggttttaa gttgcttctc aacgatcagg agttttctat gaaaagtctt accgttgaag
     2701 caaaaaatgc ttttactgat tttgtctatg atgtgaacca taagctgatg ggcgattttg
     2761 ttagtatgag taatgatgaa atcctgcgtt ttcagaagat ggaaaagctt ttagaaaata
     2821 aaatctcccg tcgtttcaaa atccgaagat tagataagga tgacttcggc tatctgattg
     2881 aacaccttta cggacagaca ggcactgcct atgaagagta tgagtatcct ctatcaaaga
     2941 aaaagctgga acatgaaaca ctgattaaaa cctatgacct cattaagcct actcgctgtc
     3001 tggtggaaga aaaacagcga tatttgaaaa tccagcagga agacgaaacc gcctatgtag
     3061 cttactttac catcaacagt attgtcggcg aactggactt cccgtcctct gaaatcttct
     3121 actaccagca acagcaattt acctttccga ttgatacgtc aatgaatgtg gaaattgtag
     3181 cgaatcgtaa agccctatct actgtccgca ataaaaagaa agaactgaaa gacttggata
     3241 accacgcttg gcaaagtgat aatgaaacca gctctaatgt ggcggaagct ctggaaagtg
     3301 tgaacgagct ggaaaccaat ttagaccaaa gcaaggaatc tatgtataag ctgtcctatg
     3361 ttgtaagggt atcagcaaat gaccttgacg aacttaaacg tcgttgtaat gaagtgaaag
     3421 atttctatga tgatttgagc gtgaaactgg tacgaccttt tggggatatg ctgggcttac
     3481 atgaagaatt tttacctgcc agcaaaagat atatgaatga ctatattcaa tacgtgacct
     3541 ctgatttcct cgctggttta ggttttggtg ctactcaaat gctgggtgaa aatgagggga
     3601 tttatgttgg ctacagctta gatactggac gcaatgtcta tctgaaacct gctcttgcca
     3661 gtcaaggggt taagggttca gtaaccaatg cgttagcgtc tgcctttgtc ggttcgctgg
     3721 gtggtggtaa atcctttgcg aataacctta tcgtctatta tgcagtgctt tatggggcac
     3781 aagcagtgat tgtagaccca aaagcagaac gtggcagatg gaaagaaacc ttgccagaga
     3841 tttcccatga aatcaatatc gtcaatctga cttctgatga gaaaaacaaa ggcttacttg
     3901 acccttatgt aattatgaaa aataccaaag attctgaatc actggctatt gatattttga
     3961 cattccttac ggggatttcc tctcgtgatg gggaacgctt cccaatcctt agaaaagcca
     4021 ttcgtgcagt aaccaatagt gaagtgcgag ggttgatgaa agtgattgaa gaattacggg
     4081 ttgagaatac gccactaagt accagcatag ccgaccatat cgaaagtttt acagactatg
     4141 actttgccca tctgcttttt agtgatggtt atgtagagca gtctatcagc cttgaaaaac
     4201 aactgaacat tatacaggtt gccgacttgg tacttcctga caaggaaact tcctttgagg
     4261 aatataccac aatggagtta ctatcggtag caatgctgat tgtcattagt acctttgcgt
     4321 tggactttat ccatacagac cgaagtattt tcaagattgt agacttagac gaagcatgga
     4381 gcttcttaca ggtggcacaa gggaaaacac tatctatgaa gctggttcgt gctggtcgtg
     4441 ctatgaacgc tggggtatat ttcgtgaccc aaaatacaga tgacctctta gatgaaaaac
     4501 tgaaaaataa cctcggctta aaatttgcct ttcgttccac tgaccttaac gagattaaaa
     4561 aaacccttgc cttttttggt gtagacccag aggacgaaaa caatcagaag cgattgcgtg
     4621 atttggaaaa cgggcaatgc cttatcagtg atttatatgg tcgtgtcggt gtgatacagt
     4681 tccaccctgt atttgaagaa ctgctccatg cctttgatac cagaccacct gtgcgaaaag
     4741 aggtgtaaat gtgaaaccat caatattaaa cagaataaaa tcaaactgga cgctgaaacg
     4801 tctaggtaaa gtggcaatga cagtggcttt cacacttgtg attgccattt ttcttttagc
     4861 catgctggga acggtggttc aagctgcggg cttggtagat gatacggtca atgtggcaaa
     4921 tgaatacagc cgatacccac ttgaaaacta tcaactggat ttttatgtgg ataatagctg
     4981 gggctggcta ccgtggaact ggtcggacgg aattggaaaa caagtcatgt atggactata
     5041 tgccattacc aattttattt ggacaatcag tctttatgtt tccaatgcga cgggttactt
     5101 agtacaggaa gcctattcct tagacttcat ttccgctaca gcagattcca ttggcaagaa
     5161 tatgcagacc cttgcagggg ttacgcctag tggactatca acagagggtt tctatgttgg
     5221 attcctttta ctcttgattt tggttcttgg ggtttatgtt gcctatacag gactgataaa
     5281 gagagaaacc acaaaggcaa ttcatgccct catgaatttt gtgctggttt ttatcctatc
     5341 ggcttccttt attgcctacg ctcccgacta cattaaaaaa atcaatgact tttcatcaga
     5401 catcagcaac gctagtttgt cactgggtac aaaaattgtc atgccccatt cagatagtca
     5461 aggcaaggac agtgtggact tgatacgtga cagcctattt tccatacagg ttcagcaacc
     5521 gtggctactg cttcaatata acagttcaga cattgaaagt attggtattg accgtgtgga
     5581 aagcctgctc tcaaccagtc cagattccaa caatggggaa gacagagaaa aaattgttgc
     5641 ggaagaaatt gaagatagaa gcaataccaa tatgacgatt acaaagacga ttaaccgttt
     5701 aggtacagtc ttcttcctat ttgtcttcaa tattgggatt tccatatttg tattcctatt
     5761 aacaggaatc atgattttct cgcaggtact ttttatcatc tatgctatgt ttctgcctgt
     5821 gagctttatt ttaagcatga ttccatcatt tgatggtatg tcaaaacgag ccataacaaa
     5881 gctctttaat accattttga cacgagctgg aatcacattg attattacga cagcatttag
     5941 tatttcaacc atgctctata ccttatcggc tggttatccg ttctttttga ttgcttttct
     6001 acagattgtg acctttgcag gaatctactt taagctgggc gatttaatga gtatgttttc
     6061 tctacagagt aacgattctc aaagtgtggg aagtcgtatg atgagaaaac ctcgtatgct
     6121 tatgcacgct cacatgcacc gtctacagcg gaaacttgga cgctccgtga ctgctttagg
     6181 ggctgggtct gccattgcca gtgctacggg taaaaaagga cagtcgggtt cgggaagttc
     6241 tgcgagtaca caagcagatc actctcgacc aaacgggcag gaaaaatcaa cactaggaaa
     6301 gcgtatcggt caaaccatcg gtacagtagc tgataccaaa gacagaatgg tagatactgc
     6361 tggtaatttg aaagaacagg ttaaggattt gccgaccaat gcaagatatg cagtatatca
     6421 aggaaaatct aaagtgacgg ataatgtcca tgatttaacc agaagtattt ctcaaaccaa
     6481 agcggataaa gccagtggac gcaagaaaca gcaggaacaa agacggaaaa ccattgcgga
     6541 gcgtcgctct gaaatggaac aggtcaaaca gaaaaaacag ccagcttctt ccgttcatga
     6601 aagaccagcc actaagcaag aacaatctca tgatggacag acttcaaggc aaacaactgt
     6661 acaggcttcc tatagggaat ctcaacaagc caaacaagag cgtccaacag ttaagtccga
     6721 ttcttcaagt ttaaaaacgg aacgtcaaag taatacagtt caagaaagaa cggttcaaaa
     6781 gccagtaact tcaactgcac caacagatag agcttcacaa cgttcaatca caaaagaacg
     6841 tcagtctact gttcaaagag taccactaca aaatacaaaa agtagaccac caatcaaaac
     6901 cgccaccatt aagaaaggca gtaagaaacc atgaagctga aaactttagt gattggtggt
     6961 tcgggattat tcttgatggt cttctcactg cttctgtttg tcgccatttt attttcagat
     7021 gaacaagatg gcggtttttc caacattcac tatggcggtg tggatgtttc cgcagaagtg
     7081 ctggctcata agcctatggt agaaaaagtt gccaaagaat atggaattga agaatacgtc
     7141 aatatacttc ttgcgattat acaggtggaa tcgggtggta cagcagaaga tgttatgcag
     7201 tcctcggaat ccctcggtct tccacctaat tcattgagta cagaagaatc cattaagcaa
     7261 ggtgtgaagt atttcagtga gttattagcc agtagcgaaa ggcttggtgt agatttagaa
     7321 tcggttatcc agtcctacaa ttatggtggt ggtttcttag gatatgtggc taatcgtggg
     7381 aataaatata cctttgaact ggctcaaagt ttctcaaaag agtattcggg tgacgaaaaa
     7441 gtgtcttacc ccaatcccat agccatacct atcaatgggg gctggcgata caattacggg
     7501 aatatgtttt atgtgcaact ggtaacgcag tatcttgtca caacagagtt tgatgatgat
     7561 acagtacaag ccatcatgga cgaagctctg aaatttgaag gctggaagta tgtctatggc
     7621 ggagcttccc cgactacctc ttttgattgt agcggactga cacaatggac gtatggaaaa
     7681 gctggaatca acttaccacg aacagcacaa cagcaatatg atgtaacccg gcatatccca
     7741 ttattggaag caaaagccgg cgatttggtt ttctttcatt ctacctataa cgctggctct
     7801 tatattactc atatcgggat ttatttaggc gataaccgta tgtttcatgc aggcgaccct
     7861 atcggttatg ccgacttaac aagctcttac tggcaacagc atttagtggg agcaggacga
     7921 atcaaacaat aagaaaggac gattcaatga tgaaattcgg aaaaaatcag aatacagaaa
     7981 aacagacacc aaaggaaaag aaacctcgtg tctataaggt caatcctcgt aaaaaggttg
     8041 tgattgtcct atgggtactt ttaggactta gtttcagctt tgcgatattc aagcacttta
     8101 cagccattga tacccatacc attcatgaaa ccaccatcat agaaaaggaa tatgtcgata
     8161 ctcatcatgt ggaaaatttt gtagagaact ttgcgaaagt ctactattca tgggaactaa
     8221 acgacaagtc cattgataat cgcatagaaa acctgaaagc atatctgaca gaggaacttc
     8281 aagctctgaa tgtcgataca gttcgtaaag atattcctgt atcgtcttct gtaaaaggat
     8341 ttcagatatg gacggtagaa gcagatggcg acaatcaatt tgatgtaacc tacagtgtag
     8401 accaactcat tacagagggg gaaaatacaa agaccgtcca ctctgcttat atagtgagtg
     8461 tctatgtaga cagtactgga aatatggtcc tcattaagaa tccgaccatt actagcatac
     8521 caaagaaatc agactataaa ccaaaagcta ttgaaagtga gggtacggtt gattccatta
     8581 caaccaatga aatcaatgag tttttaacga cgttcttcaa gctctatcct acagcgacag
     8641 ccagtgaact ttcctactat gtgaatgacg ggatattaaa accaatcgga aaagagtaca
     8701 tctttcaaga actggtaaat cctatttaca atcgtaagga taatcaagtc acggtatcgc
     8761 tgacagtgga gtatatcgac cagcagacca aagcaacgca ggtatctcaa tttgatttgg
     8821 tacttgaaaa gaacgggagt aattggaaga ttatagaata acaaatattg gtacattatt
     8881 acagctattt tgtaatcacg tactctcttt gataaaaaat tggagattcc tttacaaata
     8941 tgctcttacg tgctattatt taagtatcta tttaaaagga gttaataaat atgcggcaag
     9001 gtattcttaa ataaactgtc aatttgatag tgggaacaaa taattggatg tcctttttta
     9061 ggagggctta gttttttgca cccagtttaa gaataccttt atcatgtgat tctaaagtat
     9121 ccggagaata tctgtatgct ttgtatgcct atggttatgc ataaaaatcc cagtgataag
     9181 agtatttatc actgggattt ttatgccctt ttgggctttt gaatggagga aaatcacatg
     9241 aaaattatta atattggagt tttagctcat gttgatgc
//
LOCUS       DAPBAB010000087         9103 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPBAB010000087 DAPBAB010000000
VERSION     DAPBAB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.092"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..153)
                     /locus_tag="RD444_002538"
     CDS             complement(<1..153)
                     /locus_tag="RD444_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7549861.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(296..481)
                     /locus_tag="RD444_002539"
     CDS             complement(296..481)
                     /locus_tag="RD444_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549862.1"
                     /translation="MDDVLTDIQNLGYEIILVSFGAIVIGANKLNFHKYPETLKIKWL
                     AQLSLHNKNTFEIISKK"
     gene            complement(574..1116)
                     /locus_tag="RD444_002540"
     CDS             complement(574..1116)
                     /locus_tag="RD444_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDT7549863.1"
                     /translation="MINVSEIKTNKPQKYETLLQEKVYAALESLDISYERVETDEVIT
                     MEDCEQVNLKLDMHMVKTLFLCNRQKTNYYLFITSGDKPFKSKDFSKALGISRVSFAS
                     KEDMTSMLQTKIGAATVFSTLIDTKNKIKIIFDKDVTDDRWYGCSDGTTTGYLKISTN
                     EIIAKLLPYTKHQMEIVKIS"
     gene            1238..2104
                     /locus_tag="RD444_002541"
     CDS             1238..2104
                     /locus_tag="RD444_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7549864.1"
                     /translation="MDMNIQKYIAFLKTVELKSFTKAAEALNYSQSGISRMINDLEDE
                     WGVTLLERNRTGLRLTSDGINIYPYVKNLFDEYESMQRKLEELNGIKFGVIRIGTFSS
                     VATHWLPQLILNFQNKYPNIKFELLLGDYDEIEHWILTGRVDFGFLRLPTLPKLSTQF
                     IHQDDFKVIVPKNHFLEKKDSVSLNDLSGLPFILLDKNGNSNITTLFETMDFSPDINF
                     TTWDDYAIMAMVEKGLGVSILPELILQRVQYDLTILDLDVPLYRKIGIAYKEEKNLSL
                     ASKKFLDLLFFE"
     gene            complement(3126..3269)
                     /locus_tag="RD444_002542"
     CDS             complement(3126..3269)
                     /locus_tag="RD444_002542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549865.1"
                     /translation="MKKYNRSYGEFEEKATRKEFMTVLQWVAEESNRKQRKIAGLDKE
                     EEQ"
     gene            complement(3554..4579)
                     /locus_tag="RD444_002543"
     CDS             complement(3554..4579)
                     /locus_tag="RD444_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HDT7549866.1"
                     /translation="MKTIAVVGGHGQVGKGNVRELTNHGYFVVLMGRNLKKMEVFAQE
                     FLPILEQSEVDLNKFVIPEKLADLDMVIVCLDQTDTQFVEQCQKLGIDYLDISANSDF
                     LKKVNLLPIPEKSKRVIGLGLAPGVTNLAAAAYTKKEPSAQMIVIDVLLGIGDRHGEA
                     AVRWTFEQLNRTYIHPKWAQPIENFVYSRKTDFGPPFGKRRSANFGFADQHLLFSADP
                     SRNYVTFLGLDQNWLTALLRTIKKVGLARLFKNPRVIKSPVWFMKRGLVGDRSFVVKV
                     SNGQVKDPEKIVLFGEEEAKMTAEIAVFVANKILIETLPNGHYHLTELTTMSEILSQL
                     PRLSATE"
     gene            5238..5597
                     /locus_tag="RD444_002544"
     CDS             5238..5597
                     /locus_tag="RD444_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7549867.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
     gene            complement(5670..6488)
                     /locus_tag="RD444_002545"
                     /pseudo
     CDS             complement(5670..6488)
                     /locus_tag="RD444_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(6902..7333)
                     /locus_tag="RD444_002546"
     CDS             complement(6902..7333)
                     /locus_tag="RD444_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7549868.1"
                     /translation="MLNKRIANKNDYKLVLEIWEKSVKQTHDFLAEADFNFYKAIIPE
                     NLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFIGKRYGSQILIELIETEDIKRI
                     DVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITHLVKK"
     gene            7434..7733
                     /locus_tag="RD444_002547"
     CDS             7434..7733
                     /locus_tag="RD444_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549869.1"
                     /translation="MTKIYFMLTSNSGVGSTTIFATRARRNIDDLLAFFSQYYDVSAN
                     YTKDKDMIDLLVLPEPFGPFNNERNLPVIQIPTILFLERDFEKIKTHIDAYLAKK"
     gene            complement(7778..8428)
                     /locus_tag="RD444_002548"
     CDS             complement(7778..8428)
                     /locus_tag="RD444_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDT7549870.1"
                     /translation="MPKVAVMAGTPIDTRMGADLLNEISCELLEVPISGNPAQQTYFQ
                     TLPVFEKMHQIEVKIKDLQSKKIDLLLVYCNSLSASIDFDFLAKKYSMKIITPFQVYA
                     DLAQKHERIGVLTANGQGAAGIESVLVQHHSTIRVYNVTNLDWVHAVEAKIHPSIIVE
                     QFGLTDSMSFFMKNQVEAILIGCTHFPYFLTEYQSRTRIPCINPDDALIEKVKSIL"
BASE COUNT         3023 a         1586 c         1276 g         3218 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcaacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaacccttt tattgaaagt
      241 aagaagaaca ggctttttta ttagaggcgt tcaaaattgt ttgagcggtt agcttttatt
      301 ttttagaaat aatttcaaaa gtatttttgt tgtgtaaaga aagttgtgcc aaccacttta
      361 tttttaatgt ttcaggatat ttatggaaat ttagcttatt tgcaccaatc acaatggctc
      421 cgaatgaaac cagtattatc tcatagccaa gattttgaat atcggttaaa acatcatcca
      481 ttttatcgaa agattagata ttatttttac caattgtatt tgttaaagtt gtatttccaa
      541 ctttaattac aatcctgtct ctatgaatgt tcatcaggat atttttacaa tttccatttg
      601 atgttttgta tagggtaata actttgctat tatttcattt gttgaaatct tcaaatatcc
      661 agtagtagtc ccatcactac aaccatacca cctgtcatcc gttacatctt tatcaaatat
      721 gattttaatt ttattctttg tatcaattag tgtgctaaaa actgtagctg ctccaatttt
      781 ggtttgtagc atacttgtca tatcttcttt ggaagcaaaa gacactcttg agattcctaa
      841 agctttacta aaatctttag atttaaatgg tttgtcgcca ctcgtaataa ataaatagta
      901 atttgttttt tgtctattac acaaaaagag tgtttttacc atatgcatat ctaatttcaa
      961 attcacctgt tcgcaatctt ccatagttat cacttcgtct gtttctacac gttcatacga
     1021 tatgtctaaa ctttctaaag cagcgtaaac tttttcttgt aaaagtgtct catatttttg
     1081 aggtttattg gtttttattt cactaacatt aatcaatctg aatccttctt tcacttaata
     1141 tttactacaa agaaacttta tcatgtataa ttacatcaca aaagagaata tatcagatct
     1201 ttagtatgat aaaaccagat attaatgagg tgtaagcatg gatatgaata ttcaaaagta
     1261 tattgctttt ctaaaaacag ttgaattgaa aagttttact aaagcagctg aagctttaaa
     1321 ttattctcaa tcaggaataa gtaggatgat aaatgatttg gaggatgaat ggggtgtgac
     1381 attacttgaa cggaaccgaa caggtctacg tctaacttct gacggaatta acatttatcc
     1441 atatgtaaaa aatttatttg acgaatatga atctatgcag agaaagctag aagaactgaa
     1501 cggcattaaa tttggcgtaa ttagaattgg gaccttttca agtgtggcta cgcattggct
     1561 acctcaactt atcttaaatt tccaaaataa atatccaaat ataaaatttg aattattact
     1621 tggagattat gatgaaatcg aacattggat tcttactggt agggtagatt ttggatttct
     1681 aagattgccc acactgccaa aattaagtac gcaatttata catcaagatg atttcaaagt
     1741 tatagtaccg aagaaccact ttttagaaaa gaaagattct gtttcactca atgatctctc
     1801 tgggttacct tttattctct tagataaaaa tggaaactcc aatatcacta cattatttga
     1861 aacgatggac ttttcacctg atattaattt cactacttgg gatgattatg caattatggc
     1921 catggttgaa aaaggattag gtgttagtat acttccggaa ttaattttac aacgtgtaca
     1981 atatgatcta acaattttag atttagatgt tccattatat agaaaaatag ggattgcata
     2041 caaagaagaa aaaaatttat ctttagctag taaaaaattt ctagatcttt tattttttga
     2101 ataattcatc aaaagtctta gctcaaaata tatttacaat cataaaatca aatcgttttc
     2161 agactatttt gataaaatac gagatagagc ctaatatcat tacctgacca ctaccgatca
     2221 taataattac ttataacaag attgagttta tccgaaacta ttccaaaaga aatttatata
     2281 ctaatcacac ttcactaaaa ttatttcacc aatacaaata ttttcctata agcaagtcta
     2341 aaaaaactgc tataagacaa aaaaatagcg atttacgatt tttttaccac aatcagtatc
     2401 gagtttaata taatagtatt atcaaaaagg gagtgtgatc aacatgcaaa atataaaaaa
     2461 aatactatta gtagaaatga aacaagttga aagaaacgaa gctgttaact gtgttgcagg
     2521 agctatcagc gaaggactag ctagtattga ttttgaagct gctagggtct aatgatatgt
     2581 ctactaaaaa caaatccaaa ggacttatag tatcaactat tattttttca tgctttattc
     2641 tattaggtat ccataatttc agcacaaacc tttcaatccc aaaattatta ttaaacagtt
     2701 tagggatctt cttgtttatc cttttactct ttctttcgat aagagaatta ttcaaacggt
     2761 aaaaactata ctgtttgttg caacaatttt atttatctaa taaaaaaaga tagtcttcaa
     2821 agaaaaagct attcttaaca tatggcattt tcatattttt aattacctat gacgttctat
     2881 gggagattta ttctcattat gatacaaaat aaagttatat aatttgaaga acacttaaat
     2941 agccttagta ttgcacacaa aagccgacta cttcaattta tgatgtagtc ggtctttttt
     3001 aaatattatt tgtactagat actcactatc agtagcttct tgtaaaacag attttaggat
     3061 tacaatttaa aattcattta cttccttttt aggcattttt actaagaaga acagtgggct
     3121 tttgcctact gctcttcctc tttatccaat cccgcaatct ttcgctgttt ccgattagat
     3181 tcttcagcta cccactgtaa aacagtcata aactcttttc gagtagcctt ttcttcaaat
     3241 tctccatagc tgcgattata ttttttcatt atttcctcag cttcttctct ttcaatcaag
     3301 taacttcaca tccaattcgt tttaagtata agtgtctcgc aatataaggt cgatatctgt
     3361 cacagcaaga actattattg taatctggtt gatatagaat tttattacta tagaaaaaat
     3421 taaatactat aatttaatct aaaaaagcta ttgcatacta caacatattt gttgaaatga
     3481 tagaataaaa gaataatttt aacaaattag agaagcttgt ttccattttt catagtgtaa
     3541 aaagtttttc aatttattct gtggctgata aacgtggaag ctgacttagt atctccgaca
     3601 tggtagttag ttctgttaaa tggtaatgcc catttggtaa tgtctctatt aaaattttgt
     3661 tcgcaacaaa taccgcgatt tctgcagtca tttttgcttc ttcttctcca aagagaacaa
     3721 ttttttcagg atctttaact tgtccatttg atacttttac aacaaaagaa cgatctccga
     3781 ctaatcctcg tttcatgaac catacggggc tctttattac tctcggattt ttaaaaagtc
     3841 ttgctagtcc aacttttttt attgttctta acaatgcagt caaccaattt tgatccaaac
     3901 cgagaaatgt tacataattg cgtgaaggat ctgcagaaaa aagtaaatgc tgatcggcaa
     3961 agccaaagtt agcactgcga cgcttcccaa atggtggtcc aaaatccgtt tttcgactat
     4021 agacaaaatt ttcaattggt tgtgcccatt taggatgtat atacgtacga tttaattgct
     4081 caaaggtcca tcttactgct gcttcaccgt gacgatcacc tattcctaaa aggacatcaa
     4141 ttactatcat ttgagcagat ggctctttct tagtgtacgc ggccgcggct aagttggtaa
     4201 caccaggtgc taaccctaaa ccaatgactc tcttgctttt ttcaggaatg ggtagcaaat
     4261 taactttttt caaaaaatca ctattggcac tgatatccaa ataatcaatt cctaattttt
     4321 gacattgttc gacaaactgg gtatccgttt ggtccaaaca aacaattacc atatcaagat
     4381 cagcaagttt ttcaggaata acaaatttat tcaagtctac ttcactctgt tctagaatgg
     4441 gtaaaaattc ttgggcaaat acttccattt tttttaaatt tcttcccatt aaaacaacga
     4501 aatacccatg atttgttaac tctctaacat taccttttcc aacctgtcca tgaccgccaa
     4561 ctactgctat cgtcttcatc tttcttctcc tttatctacc aagttaattc atttttaccg
     4621 ataggctaaa tcgtataaaa actaaccact tttaatcatt attttattct tagttttatg
     4681 tgagataaat taaccatttt aagtttacaa atgtaatcaa tttctattac tttataagta
     4741 tcgtttacat tcgtcaacga tagagcttga aatattttta tttgaaaatt ttgtatttag
     4801 atgattatga actaatacag tgtcggagga tacctatgta tttaaactaa actattttag
     4861 aaaaaccagt aatcctttga attttctatt ggaaaatgaa gtaaaatatt ataagaataa
     4921 cctttaaagt tgggcaacta ttattcaaac atctaaacat actcctaaga gaaatagata
     4981 tataatagac taaggaagcg ttttaaatat tgtagaaaat agagaaaatt tatcagatct
     5041 tcggtttaat ttttacagcg ttaatcgctc ttgatatgaa tgagctctga taaagccgaa
     5101 gtaaattggc gatttgaaaa gttcaattat ttgagaatag aatatttttt atatagaatt
     5161 gagttatagt taagggggaa taggtgtgtt aaataaaatg tcaatttcaa ctgtggaagt
     5221 cgatggtggt cgtgaaaatg agcgataatt tatttttaga agtacaacca gaagtattta
     5281 agcaagtacc tcgtttaaag gatggattct tgttaacttt aaatgatggc tctaatcaat
     5341 tttcttcagc agagggatgc tgtatgattg gtgatcattt tttattagtt tccacacaac
     5401 attatattcc agcatatgat ttaacaatac cttgcgaaga agcaacagtt aaaacatctg
     5461 aatacgatac tacctttatt aatgggcatt tagtattaga ggtaactcca tcaaatcaaa
     5521 caattcgttt aaagaatgaa agtggtattt tagatagcaa tgtccaagtc tattttgact
     5581 catcagacgc ttcgtaaatc tgttctaaat atagttttaa aacctcgaat atttcttata
     5641 tcgaggtttt ttgatgaatt acctagtgtt tacgtgttaa ggaacaatcg atcgagcgtc
     5701 actaaaacac cgtcttcgtc atttgaagga cattgatgct tagcaacttg cttaacagct
     5761 tccgttgcat tatccattgc ataactcaga ccacaatact ccaacatctc aatgtcattg
     5821 cccccatctc caaaagcaac gcactggtca ggattaatcc ccaagcgctc aactaacatt
     5881 ttcaagccga aagttttatg gcactcagaa agaatcaagt ctacagaacc atgaccacta
     5941 ctggtaggta ctaatagccc ggctaatttt tcacggaaca attcaacgta aaattcggtt
     6001 ttttcaacag gcatagtcgg agcaaatttt aaaattttat cttgaacttc tgcaaaatca
     6061 gctacccatt ctaatctatg gtaatagata ttggttagat caaaaaatgc ttgactcacc
     6121 tttcctcttt cacagtaggc actattcaga ccacacatca cattttttac ttcaggatag
     6181 tctttacata aatcaaggac cacctcgatc gcttttctag gcatatcggc tgaaaatagt
     6241 acctctgttt tatctttaac aaaagcacca ttttcagcaa caaaacttaa ttcatcataa
     6301 taatttggaa ataacgatct caattggtag tattgatttc cacttgcaac aacaaattga
     6361 caatcaacag ctttcattca cgataaaaca gccttgaacc ttgggatatc gtatgtgtaa
     6421 tcagtacgag caaatgttcc atccatatct actgctacca actttagatt atgaggattc
     6481 ttattcataa aaacactcct gtcgcctttg atatcttcat tatattgaaa aagtattcag
     6541 acctaaagca gcctattttt cttactccgt taaaaataag aacatttcta atttcagtta
     6601 actatctaga tacatacaag ccattttttg tattaaaagc taccgacctg tctttctcct
     6661 aacccacagg tcagcctaaa tcagtgttga cataagattt ttatcatttt tactttaact
     6721 gattttctat aactggtaca tccttggtca gctagtatca taagttgatc ctaagataag
     6781 caccttatta ctgaatgtaa tacacaaatt tgatctggat aattcataac ctagaaatag
     6841 gccatcaaaa taaaaatatt acttttattc atgttcaatc aggtaataaa ccattcatga
     6901 attatttttt tactaaatgc gttatagggt atggtttacc aaaaccatct aagtcttctt
     6961 cagattcaat ttcaaatcca tggcttaaat aaaaatgttt tgcatattta ttttgactat
     7021 taacatcaat tctttttata tcttcagttt caatcagctc aattaatatt tgactaccat
     7081 atcgcttacc aataaaatct ggatcaagaa atagcataac taattcttct gcacttacac
     7141 cactaaaacc gacaatttct ccgttatctt cccataaata aagtttaacc tcatctaagt
     7201 tctcaggaat gattgcttta taaaaattaa aatctgcttc agctagaaaa tcatgggttt
     7261 gctttactga cttctcccaa atttctaaaa ctaatttata atcattttta tttgctattc
     7321 ttttgtttaa catgctctta ttcccctttt cttataattt taactttatt tgcaagcata
     7381 gttagtcaaa cataaagcta ccgctatatt ttatttaaaa aggagaattc gtaatgacta
     7441 aaatctattt tatgcttact tctaatagtg gcgttggttc taccacaata tttgcgaccc
     7501 gcgctagacg taacattgat gatcttttag cttttttctc tcagtattat gacgtatcag
     7561 caaattatac caaagataag gatatgattg atctattggt gctaccagag ccctttggac
     7621 cgtttaataa tgagcgtaat ttgccggtca ttcagattcc gactatttta tttttggaga
     7681 gagactttga aaaaataaaa acacacattg atgcttattt ggctaaaaaa taaaacaaaa
     7741 aaatcctaag ttattgtctg tgtcaaacca atgcttatta aagtattgat ttgacttttt
     7801 ctattaatgc atcatctgga ttgatacaag gaattctcgt tctggactga tattctgtaa
     7861 gaaaataagg aaaatgagta caaccgatca aaatcgcttc gacttgattt ttcataaaaa
     7921 aactcattga atccgtcagt ccaaattgtt ctacaataat actagggtgg atctttgctt
     7981 ccaccgcatg tacccaatcc agattcgtga cattataaac tcgtattgtt gaatggtgct
     8041 gcactaaaac actttctatt ccagccgctc cttgaccatt agcggtcaac acaccaatac
     8101 gctcgtgttt ttgagccaaa tccgcatata cttgaaatgg tgtgataatc ttcattgaat
     8161 atttcttagc cagaaaatca aaatcaatcg atgcactcag tgaattacaa taaaccaaca
     8221 gtaaatcaat ttttttactc tgtagatctt ttattttcac ttcaatttgg tgcatctttt
     8281 cgaagactgg aagtgtttgg aaataggtct gttgagctgg atttcctgaa ataggtactt
     8341 ctaacaattc acaggaaatt tcgttcaata gatcagctcc catcctagta tcaataggtg
     8401 ttcccgccat tacagccact ttaggcatac tgatcattcc ttcaaaatga atttttgcct
     8461 attatactct aaaaatcaaa tttgaagaat agcgatccgg aagttaaact gaaaattcta
     8521 aagtaattct ttgatttatt tagattcaac ttaacatctt tagtgtacta ataaaaaatt
     8581 cgctcaatat ttctagctat actttatatc ccaatataca taagcttccc tatttttaca
     8641 ggttacaagt gaaaattaaa ctgcttatat tgattgcatt taactactaa ggatgatgct
     8701 tgttgttagt atgtcagaaa aatccaacag tatagttttg tcagcacatt catcattctt
     8761 tcctgtaaaa caaaaagaga gtagacctat tcttccactt catcatcgtt atctaaaaca
     8821 gcaattttct gctgtaaaat agtcataaat tttttctagt agccttctct ttaaactcat
     8881 cataatctca attatctttt tttaagattt gctcaacttt ttccctcttg attattgcaa
     8941 ttcacacctt ataaagtttt aaactaatgg gagacgtcat ctagcttaac ttatagtccc
     9001 tgctccgttt aatctacact tgttatcttg tattattgat aaaagataga ctagttacgg
     9061 attgaaatta ggttgcaatt ctaaaaatcc agtagtctaa aat
//
LOCUS       DAPBAB010000088         8792 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPBAB010000088 DAPBAB010000000
VERSION     DAPBAB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.093"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(28..282)
                     /locus_tag="RD444_002549"
     CDS             complement(28..282)
                     /locus_tag="RD444_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549871.1"
                     /translation="MKKVFKIFLWIAFITLTIATFFFAVAASYGTILAWFMNGLLFNE
                     NLFAFKWFMFQVICLILTATLRYGVGKLCKKLSMEEDEIQ"
     gene            complement(365..1024)
                     /locus_tag="RD444_002550"
     CDS             complement(365..1024)
                     /locus_tag="RD444_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549872.1"
                     /translation="MLYILTLLFATIGSSTLIAGIFIVKNMFIVFLGLFFLLFLLFVP
                     RIRGVKSNYEKIIEKELIQSHYWFSQDFRSNMNIEVTIGEIHKNGKVDFIVFVTDDKY
                     LFKKYLIGSYDKEQLTNIQIVNIEFLKLPKIFSALEEESIYFYKLHSKSSKERFYAEG
                     ITTNNQKVEIIASNEDIEIKIDDKTKTTKKLLEITNSYVDIESQGLIVATTRNSKMSK
                     F"
     gene            complement(1229..1519)
                     /locus_tag="RD444_002551"
     CDS             complement(1229..1519)
                     /locus_tag="RD444_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549873.1"
                     /translation="MKFFKVKAPFYYALIAAKDKEECMDLYDKVVTDIEDREEFIRCL
                     QELDRNEAIEKDAKTISEETLEPIGMEEATKEIFSIINEQKSCVLSIDSALV"
     gene            complement(1535..1876)
                     /locus_tag="RD444_002552"
     CDS             complement(1535..1876)
                     /locus_tag="RD444_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDT7549874.1"
                     /translation="MKNYDPNMRRGTHTIKVSFQRWEYKGFVMFRKGGNCKGLSVLSL
                     DEEDLYYQPLTDNPIGFGLLPEDDEGNEWFKMTLKNDEGDELLVEDVWEELGDYIVGL
                     EIVTFVADKEQ"
     gene            complement(1863..2192)
                     /locus_tag="RD444_002553"
     CDS             complement(1863..2192)
                     /locus_tag="RD444_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549875.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDITTIIEGILYGEVDGEISSTKDLLEFLNKNKDRFVVLTDYEE
                     DTTNEKL"
     gene            complement(2783..3115)
                     /locus_tag="RD444_002554"
     CDS             complement(2783..3115)
                     /locus_tag="RD444_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDT7549876.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKNPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(3949..5034)
                     /locus_tag="RD444_002555"
     CDS             complement(3949..5034)
                     /locus_tag="RD444_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDT7549877.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(5263..5670)
                     /locus_tag="RD444_002556"
     CDS             complement(5263..5670)
                     /locus_tag="RD444_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549878.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(5689..6507)
                     /locus_tag="RD444_002557"
     CDS             complement(5689..6507)
                     /locus_tag="RD444_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7549879.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(6733..7026)
                     /locus_tag="RD444_002558"
     CDS             complement(6733..7026)
                     /locus_tag="RD444_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDT7549880.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
     gene            complement(7038..7217)
                     /locus_tag="RD444_002559"
     CDS             complement(7038..7217)
                     /locus_tag="RD444_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549881.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            complement(7214..7564)
                     /locus_tag="RD444_002560"
     CDS             complement(7214..7564)
                     /locus_tag="RD444_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549882.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(7557..>8792)
                     /locus_tag="RD444_002561"
     CDS             complement(7557..>8792)
                     /locus_tag="RD444_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDT7549883.1"
                     /translation="LDPLTTELVVMSRSDNTGSGLILASSPEAKKRYGITNVSRPRDL
                     PQPFPKTLHVVPPRMNLYIKRNMQVNNIFRRYVADEDLLIYSIDESILKVTKSLNLFT
                     TEETRSQRRKKLAQMIQERIKEELGLIATVGVGDNPLLAKLALDNEAKHNEGFVAEWT
                     YENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRLGVIGLQL
                     YFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQH
                     NCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVG
                     ITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGARSI
                     TRSTLVGGHAGGNGGIKND"
BASE COUNT         2624 a         1640 c         1219 g         3309 t
ORIGIN      
        1 atatttattc accttcattg atcgtattca ttgaatctca tcttcttcca tagaaagctt
       61 tttacataat tttccgacac catatcgcag tgtggctgtc aatataagac atatcacttg
      121 gaacatgaac cacttaaatg caaataaatt ttcattgaag agcaacccat tcatgaacca
      181 agcgagaatt gtgccatagg aagcggccac agcaaagaaa aaagtagcga tagttagagt
      241 gataaaagca atccataaaa atattttaaa tacttttttc acatggtatt ctccttaatt
      301 tttatttatc atcatcttta aagtactttt tactgttaga tatagagctt caaaagatct
      361 aaatttaaaa tttagacatt ttgctatttc tcgtagttgc aacaatcaac ccctgacttt
      421 cgatatctac ataagaattg gttatctcca ataacttttt agttgtttta gttttatcgt
      481 caatttttat ctctatatcc tcatttgaag caataatttc tactttttga ttatttgttg
      541 ttattccctc tgcataaaat ctttccttac tagatttact gtgtaatttg taaaaataaa
      601 tcgactcttc ttctaaagca ctaaaaattt tcggaagttt caaaaattcg atatttacaa
      661 tttgaatatt agttagttgt tctttgtcat aagatccaat taaatatttc ttaaaaagat
      721 atttgtcatc tgtcacaaaa acaataaaat ccacttttcc atttttatga atttctccta
      781 ttgtaacttc tatattcata ttcgacctaa aatcctgaga aaaccaatag tgagattgaa
      841 tgagttcttt ctctataatt ttttcataat tactttttac tcctctgatt cttggaacaa
      901 acagtaaaaa taatagaaaa aacagtccta aaaaaacaat aaacatattt ttaacaataa
      961 aaattccagc aataagtgta gaacttccaa ttgttgcaaa aagtaaagtc aatatatata
     1021 acacttcaga atctcctaca gagtttacca acattatatc taatcgattc agagtcttta
     1081 tttttttcat cctgagaaat tgcttcatac aatcatctgt tctttccttg ttttttgttg
     1141 gttaattcct tttgatactc tttcctcaac cgaaaagtag ttagagaaac tgtgctatct
     1201 gtatttattt tcatatcatc ttatctcctt aaacaagtgc agagtcaatg cttaacacac
     1261 aagatttttg ttcattgata atactaaaaa tttcttttgt agcttcttcc ataccgattg
     1321 gttctaacgt ttcttcactg attgtttttg catctttttc aatcgcttca tttcgatcta
     1381 attcctgcaa acaacgtata aattcttcac gatcttcgat atctgttact actttatcgt
     1441 atagatccat acactcttct ttatcctttg ctgcaatcaa tgcataataa aatggtgctt
     1501 ttactttgaa aaatttcatt gtctattcct ccgtttactg ttctttatct gctacaaaag
     1561 taacaatttc aagacctaca atgtaatcac ctaactcttc ccatacatct tccactaaca
     1621 attcgtcccc ttcatcattt ttcagggtca ttttgaacca ttcattacct tcatcatctt
     1681 caggaagtaa tccaaacccg attggattat cagtcagcgg ttgataatat aaatcttctt
     1741 cgtctaacga taagacgctt aatcctttgc aattgccgcc ttttctaaac atcacaaacc
     1801 ctttgtactc ccaacgttga aaagaaactt tgatcgtatg tgttcccctt cgcatatttg
     1861 gatcataatt tttcatttgt tgtatcctcc tcataatcag tcagtactac aaagcgatcc
     1921 ttatttttgt tcaaaaattc taataaatct tttgtagaag atatctctcc gtcaacttca
     1981 ccatataaaa taccttcgat aattgttgtt atatctgaaa aactaatttt tactggacat
     2041 ttatcgtcta ccgcattcaa taccgacaaa attgcttctt tttctgcaat ctctcgtttg
     2101 aatttctttg gagctaatcg catgacactc aagtcatttt caagcttttc aatccatgca
     2161 gttttactca ttttcgcaaa tttttgaatc attcctctac cacctttgct atctactaaa
     2221 ttagttttac ttcataacga atatttttat cgtttttcgt tcactcaaaa atatataaaa
     2281 taaatagact cgtttatggc gtccgaaacg agaaacccta gtagacagcg gtaaatgctc
     2341 gattgatcca ataatcaacg tcctcgcaag tatctacatg tactgcagca cacgacacaa
     2401 ttttttgtgc gtcctcccca ttacgagaag acctactcct tattccatga gtttgcgtca
     2461 aagcctagat cccttagtag ctgctcatga ttaatctagg cgctattcta acttgtctaa
     2521 cttctaaatt ccctcttgtt tactctgtgc atattattta cacaaattca tttcaccact
     2581 atccaaacaa gggcatttac tcgattgacc aattttttac gttaccaatc gtaaaaacct
     2641 accccgtcac tccccgaagg gaattgttga tagatcaagt ctactcctta tttttctaat
     2701 gattctaagt tcctttagaa gtctcgacaa tattttcaag caagcgaaaa acgataaaat
     2761 acaagttatg agaatgatca attcagctga tcaaacgccc catccctttt gcggtacaac
     2821 gcaagactcc attttttctt cgaccaatca gtacaccgtt ttggtcataa acatgtttga
     2881 tggttggata tagctctttt aaagggatag gattctttaa aattatttgc ttttgtttcc
     2941 caatgtaata aatcatttct tctgagactt tgtctttctg gattatatat ttcttacact
     3001 gcacaacggt tttactttgg ccagttgata gtttcttcgt gattttctct gtagcggtac
     3061 accgtttacc gtagtgacaa atatcttcga gactcaaaat tcctgtatat gacataaaat
     3121 aatccaccca cttactttaa atatttttaa taagtttttc ttgttatttt tcaaaaatag
     3181 agaattgatt ttcgttcatg acaaacaaaa aagagggaac gcatccaaac atgcgttccc
     3241 tctaatttca gcgttcatca tacggcaaag agccacacgg acatttttat aacattatgc
     3301 ttaggcatag attcatcgta atatcacgta tttttaacct taacacagcc aaaaaaagtg
     3361 gtatcacttt tgaatatctg ttatactgaa tccgttgcgg atagtaccgt aacactgaaa
     3421 ggtaaacgca ttaagggtga tcttaatgcc acctgtggac gtcgacgaag gttggtagcc
     3481 ttccgagtct cacagggcct ttcttttttt tgtattttca attgtccaaa tttatcgtcc
     3541 aaaagtgcag aacgaaaatc aattcatcac aagcatagca gacaattaaa tttaaaacaa
     3601 ctaaatttat acaaaaaagt gtaattgaat attagcagca ctgcctgaaa agaagtaata
     3661 tcaggctttt cagcttgggt aaataagtga attacctctc ttcatgaatt attataactc
     3721 aaaaaaatac atatgcaaac actagttccc tgtttttttg aatgaaaaat aactaaaata
     3781 tctaacgatt atgacattaa aacctacgtt tgtgattgtt ttcataaact tttaatctaa
     3841 aaactccgga ataataacta tttatttgta acatttgtaa taaaattaaa attgtaaaaa
     3901 agttctaatt taaaattgat catttttata gtgttcgctt tttatcaact actctagcca
     3961 attttccgta ctaactgttg gtaattcgtt aaatgtttct ctttgttcgt ttgcaagctt
     4021 tgccgcaata gctttttcaa taaaccagtt ttttacatac acaaaaatca aattctgcaa
     4081 attatttact ttctcagtct tttgtttttg ataagctttc agcaacgtca tatataatcc
     4141 tttatccgca tcaattccgt aagattcaag ttcttcatac acaactaccg tcttctcaag
     4201 ttcctcggct ttatgttttg cgttgtgtat tgtggtgacg gtttgttggg cttcttcgta
     4261 agtgttagaa aatgttttta tcaagcttaa taccttttct ggtacaaatg tgtttatgct
     4321 gtcttctttc atgatttcaa cgaagttatc tagcaaaact tgattttgta attggtctgt
     4381 ttcagtgtct tttaagtgtc ttttagtgtc taaagattta ttaatataat aatcttgtgc
     4441 gatttccgca tttccagtgt tatcaagggt ttcagctgtt ttttctcgac gtgcgatttc
     4501 cgcatttcca gtgttatcaa gggttttagc agtttcctct cgacgtgcga tttccgcatt
     4561 ccgagaagag gttgatttat caatagtttc gcttaaatcg tcatcgaaag aattttgaat
     4621 aaaaagatca tcggcactta tttcaggttg aagtaaatag tatttatttt tttcattttt
     4681 tttcgttttt gggttaaatt ctccttttac gactgaaatc aaattatatg tttctaactc
     4741 gtttaaaatg gtttttactt tgccttggta gcaatttaat ttgtccatta ggtctgcttg
     4801 tgtaaattcc aaatataata gaccatcatc gtcgatccat ttttcatgta aggctctacg
     4861 aaattcattt ttaaaaagca tataggctat ttttgagctg tctttcattt tagagtattt
     4921 agaattctga attaaaaaac taggaaattt ataatatttt tcaatataaa cattcgttgc
     4981 tttatatccc caatttttaa ttatcatttt ttgagattga ttttctgtag acattaaatt
     5041 tctccttctt cttagaaact tgtatatatt caggattttc aacaagtgaa ctataccatg
     5101 tattaagggt gaataaaaca ctttcagtat ttaaggtata agttatatat taaaaaaata
     5161 agtataagtt atactttaaa tacaaataat gtataactta tactttaata ttcatttatc
     5221 tagtataact tatactttaa ttgaataccg catgaaaaaa aattattttg gtaatttttc
     5281 aaactcttct tgtatttctt ctcttatgtg ttcttctttt gctactccta gtctatgctg
     5341 gatatatgag tctgctaatg tattaacaat tttttcgaaa gaaattctcc ctttttcact
     5401 ttgtaactcc gtcatatagt cttgtaaaat tgagagtttg ctatttaatg ccggagagat
     5461 ctttttagga actgatacct tgtttctttt atcaggaatt gaaactggtc ggcctctttt
     5521 tttcaatgta atatttcttg tgttactcaa tgcactattg ctaaagttat attcaccgct
     5581 tattttcaca tcttggcgtt tgttattggg taataatggc tcaaattcac cattctccat
     5641 cattttagga atatcaaaat ctttaggcat tacttatgca actccttatt atttatttaa
     5701 aaactcttgt ctagcatcta gttctgccaa aatatttata aatacttggt gtgctttata
     5761 atcccattga tcaaaatttc catttttgta ttttcttaaa tgtataccaa ttttagaata
     5821 ccttctcaat cttttttgat aatgaatgag atttttggat accacggttc cgtatttttc
     5881 aattgcttct tctaggattt cttcatcaaa atcatcattt ggttctacca tacaaggtac
     5941 aatatctata atttgcaaat caagatcgta tctatcgacc atgaattttt gataatttac
     6001 atatttgttg actccctcta aggattcttc agctgtttga aatgaaatta tcgaatagtc
     6061 tgaagctgcc atagcattat cggaaaattc gctaatggtt ggtggtacat caataaatat
     6121 ttcttgataa ttgttcttta ttggaagaag taattttttc aagacagtaa cttggtctaa
     6181 ttcattttca tgtttattag caaaagcgga gaatgatcga aaactagtgt cacatgccag
     6241 aaggtcaaga ttttcattta ccttaataat actacttgct aattctccat ttgcgacacc
     6301 atttgttata gtggttttga tatcggttat accgaaagtt cttgctatga cttgtgttaa
     6361 gttcgcttga gggtctaaat caataataag cgtttttcta tttcttatca ttgcattgtc
     6421 ataagcaagc atgctgacta tcttagtttt acctactcct cctttaaaat tgccaactgt
     6481 aaatgtagtc ccttgaaaag tatccatgta aatcctccca atctttaatt gataattaaa
     6541 gtataactta tatatcggaa atggtcaaca ttaaaagtat aacttaaata ataatatcta
     6601 aaaacgtttg gatatagcgg tttttaataa taaaagtata agttatacca tatatattta
     6661 tggtataact tatactttta ttatttaagg tataacttat accttaaata ttgcttttcg
     6721 aaaatagcta ttctagtcta tctgtttctt tacttttcgc tgctgatttg aattgaattg
     6781 atcatattca ttttctagta ttaataactt ttcgtataca tcggtcatat tcatactaga
     6841 agcaagactt agatcatata caaatgaagc aggtaatgat tttattctac gttgtcgggt
     6901 tatccagctt gcaaccgttc cttgtgggta ggcatataac tcacaaaatt gttctacagt
     6961 gattcctaaa ttcttgataa tgtaaacatt gatcgggtgc gagtaaacaa atgtattgat
     7021 agacatggtg cgtaagccta ctttctttta ttattgcgtt tgcaataata gtttaacaca
     7081 cgcattattc tagtatctac tcctatttgt aaaaacaaaa aaggattcat agagataagt
     7141 tggccataac cccaaatctc ttctgatagc tgatcaaagg ttatatactt tagctcatat
     7201 tgctggataa tgttcattct aatcgactcc atttattatc ctggtaaaat tcgatatgac
     7261 gtatttcgtc gtagtgaatt ttgtcttttc caacgtagat ccccagctca tcactaccta
     7321 agatcttgcc aacaatatct tctggatagt atccttcagc gtttcgttct tctttttgaa
     7381 tagatactga tctatttttt atgactgcag cgttcaatac ttccgcaatc tcttcagtcg
     7441 tcatttgttc ttttgctaaa ttttgactgg ttcgttgttc attgtcggca tcaatttctt
     7501 cagtatgatc agataaataa aaccctgccc actttaaacg tccacgatca ttgtaatcaa
     7561 tcatttttaa ttcccccatt tccgcccgca tgaccaccaa caagtgtgct tctagtaatg
     7621 cttctagcgc cttctaacat tgaagaagca tgtacaatcg acgtaaaacc atatttttgc
     7681 cgaatcttgt caataatttt atctaaattt tcttcgtcaa tttgcttttg tggatcacta
     7741 aataaatcca gctgaagcga atcggtgtac acaagcttgc tataggttat tccaacatgc
     7801 cttacttctt gtccttcata atacttatca aacaagaaaa gacaataatt ctgcaattcc
     7861 tttgtattat ctgtgatagg gatcttcatc tggtgtgaaa accctggtct cgtttctaag
     7921 atcgacgtgc caatgtgtaa atggacacaa cctgttttac agttatgctg cctaatgcgt
     7981 atcgctactt gttccgccat ttcttttacc accagctcga tttcatttct tctagtataa
     8041 tctcgtggta acacttgtga atttccatat gatttttcct ttgtcggttc tggtgggatt
     8101 gctatatctg ttcgatcgat cccattagca tggaagtaca gctgtaagcc aatcacacct
     8161 agtctattct taatcgtata aggattccag ttagcaagat ctctaatgct cataatcccc
     8221 atctgattga gtcttttttt cattcgagag ccaattcccc aaaagtccgt catttcagga
     8281 atattccata ctttttcagg aacgttttcg tacgtccatt ccgcaacaaa tccttcatta
     8341 tgctttgctt cgttgtctaa ggctagttta gccaaaaggg gattatctcc gacacctact
     8401 gtagcaatca aacctaactc ttctttaata cgttcttgga tcatttgagc gagcttcttc
     8461 cttcgctgac ttcgtgtttc ttcagtcgta aaaagattca gtgattttgt cacttttaaa
     8521 atcgattcat cgatcgagta aatcagtaga tcttcatcag ccacatatct tctgaaaata
     8581 ttatttacct gcatatttcg cttgatatat aggttcatac gtggtggaac aacgtgtagt
     8641 gttttaggaa atggttgtgg taagtcacgt ggtctactca catttgtaat accataccgc
     8701 ttttttgctt caggagaaga agctaatatc aatcctgaac cagtattatc agatcgactc
     8761 ataacaacaa gttctgttgt taatggatct aa
//
LOCUS       DAPBAB010000089         8722 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPBAB010000089 DAPBAB010000000
VERSION     DAPBAB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.094"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            34..279
                     /locus_tag="RD444_002562"
     CDS             34..279
                     /locus_tag="RD444_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549884.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(463..1212)
                     /locus_tag="RD444_002563"
     CDS             complement(463..1212)
                     /locus_tag="RD444_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7549885.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1398..2102
                     /locus_tag="RD444_002564"
     CDS             1398..2102
                     /locus_tag="RD444_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDT7549886.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2142..2699
                     /locus_tag="RD444_002565"
     CDS             2142..2699
                     /locus_tag="RD444_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7549887.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2707..3846
                     /locus_tag="RD444_002566"
     CDS             2707..3846
                     /locus_tag="RD444_002566"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDT7549888.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3858..4289
                     /locus_tag="RD444_002567"
     CDS             3858..4289
                     /locus_tag="RD444_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549889.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4303..5748
                     /locus_tag="RD444_002568"
     CDS             4303..5748
                     /locus_tag="RD444_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDT7549890.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            6034..7659
                     /locus_tag="RD444_002569"
     CDS             6034..7659
                     /locus_tag="RD444_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDT7549891.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            8291..8644
                     /locus_tag="RD444_002570"
     CDS             8291..8644
                     /locus_tag="RD444_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549892.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         2972 a         1235 c         1736 g         2779 t
ORIGIN      
        1 aataactgaa aaaattatta taggagagaa aaaatgtcaa aatctaaaaa aaatatcacc
       61 ataattggtt taggcttaac cgtatattta tttacagaat ttgtttatag acatgtgaat
      121 ttatttgcag aacctttatt tgtattatac gaatcaaatt cctcaatcgt cagtgaactt
      181 gctaatttaa tgatagcatt gctgtttact cccgcgttga gagtcataac tattttggtc
      241 tgtttagtgc tattgttttt tgttaataaa aagaaataac gtagtatttc aaaagacaaa
      301 gaagtgggat gatgattgct atagtatgtt atacgtggtg ctaggtaagt gagaataaac
      361 aaaaaaggaa acacgcaata aagtttattt caaaacttta ttgcgtgttt cttttttaga
      421 ttttttatat ctgttgcttt agtagttacc aatagttttc gcttaatcaa tcaaaacgat
      481 tttttgttta ttgtattctt tacgaacctt ttcaggaatt tcttctgaaa taagataggt
      541 aaggtcttca attggaccga cgatatgatt actgatggtg ccgaatttat cattagttat
      601 catcccaagt acttcagtag aaatacgtat catctcacgt ttaatgtcgg attcttgcat
      661 agttggcacg ctcgttccaa catctagatc tatgttataa atacccataa tataaaggtc
      721 ggctcgaatg ttttgtagtt gcttataagc ttgtacccca atattgatca tagattgttt
      781 ataaaaagta ccgccaatat tgataatttc caaattagga tgctgtgcta atgctatggc
      841 aatcggcggg ctattggtaa tgattgttgc acgaaaatct tgtgggattt cttttgctaa
      901 agccaagtta gttgtacttc catcaatgat gatgacagat tcttctttta aaaaagggag
      961 ggctttatga gctaatgcag ttttttgttc gctagctaca gtttctcggt agttaaaatt
     1021 ggtaattggc ggaccaacac gtaaagcgcc gctgtgtact ctttttaaga gaccttggct
     1081 atccaaatct ttcaaatctc gtcgaatcgt gtcttcggat acattgaaac gttgacttaa
     1141 atcactagcg ataacttttt gttctgcgtt taataaatct agaatttttt gatgacgttc
     1201 taatttcaac atgttgcgtg ctccttctta tttattcttg tttatcatgt catttatgcg
     1261 tttttatgtc aataatttcc gtttttaata cactaaaaag gcataaacaa gcaaaaacaa
     1321 aatattttta tgcagtttta tgttgactag tatttgttaa gcggttacaa tgagggggta
     1381 attaaaggag ggaatgtatg gagaaaaaaa ttattttgtc gccttcactt atgtgtgggg
     1441 atctagcaaa tttggaagga agcatccaag aactagaaag tattgggatg gattccttgc
     1501 atattgacgt gattgacggt tcttttagtc ccagtatgcc tttagggatc gaaacaatca
     1561 aaagaattag agaaatcact tcaatgaatt ttgatgtaca catcatggct aaggataacg
     1621 ataacgaatt ttttattgga gaaatgttga aaattggcgt agagagcatt acttttcatt
     1681 atgaaacaag cctccatatt gatcgatata tccagctcat caagaattct ggaacaaaag
     1741 tagggatagc tttgaatcca gcaactccgt taaatgtatt acaatatatt ttacccgaga
     1801 ttgataggat ttgtttgatg ctgattaatc caggttttgc aacaaacaag tcagaaaaac
     1861 aagtttctta cgcgctaaaa aaaattacag atctttgcca gttagtaaat gagaaagagt
     1921 tagcagtaga tatccaagtg gatggacggg tatcgttaga aacgatcccg ggattattag
     1981 atgcaggcgc aacaaatctg gtattaggaa gtacaagttt gtttcttaaa ggaaatacat
     2041 tagcagaaaa tcaaaaacta ctttatcaag cgatgaagca atcagttagg aaaaatcaat
     2101 agatgtagtg gtcctgaata atagaaatgg aggggaaata gttgagttat aaggcggcga
     2161 aacaaaatat tgtaagagaa attactgaaa cattagaccg tatttcttca aaagatgtgg
     2221 aacaattgat aaagaaaatt gaaaaagcgg aacgagtttt tttcgtgggg gttggcagag
     2281 tactgttagc gctagaagga atggcaaaac gcttagggca tctaggaatt cagacagtag
     2341 tcgtaggaca aatcactgaa ccggcaatta cagataaaga tttattgatt gttggttctg
     2401 gtagtgggga aagtgctttc ccactaacta ttgcaaaaaa agcaaaggaa tttgggtccc
     2461 aagttgccca tataggatca aacccagaca gcacaatgaa agaatattca gatcttttta
     2521 ttcgtatacc cgtacaaaca aaattagcgt taccagagga aatttcttct atacagccaa
     2581 tgactagcct gtttgaacaa agtgttcttt tgttaggaga tagtattgct ttagtgatga
     2641 tagaagaaaa agcaattgat atgtcaaagt tgtggcaata ccatgctaac ttagagtagg
     2701 tgaaaaatga aagcggtaca ttttggagca gggaacatcg gcagagggct tattggacaa
     2761 attctacatc agaatggatt cgatctatgt tttattgata cgaatcctga actgatccat
     2821 caattaaatc aagataaggg ttatactatc gaaattattg atgaaatgaa caggagtgat
     2881 ttcattgatc atgttgtggc actgaatagt ttatccgagc tagaagaggt gattacagca
     2941 acaaaaaatg cagatatctt aacaacatct gttggcgcaa taaatttagt aaaaattgca
     3001 ccgatcattg cgcaagcctt aagtaagcga tttgaacaaa agcatagcat caatatcgta
     3061 gctaatgaaa atgtgataaa tgcaagtgat gtgttgaaaa aagaaattta ttctttgctg
     3121 tcagaaaaag aacaagagaa ttttgatgaa tatgcatatt ttgtgaatac ggcaatcgat
     3181 cgacaatctt taggaaaagt tcttggagga aaaatgatct ctgttgtcga accttattat
     3241 gaatggataa tggatcgaca gcagttagat ccaactacat cttttgatat gaaaaatgta
     3301 aatttggttg atgatatgga gccttacatt gagcgaaagc tttatatcgt taatgctgct
     3361 catgcggcag tagcttattt aggcgcactg aaaggatata agacgattca agaagcaatc
     3421 caaaatatgg agattttcaa tctagtcaag caatttttaa tggagaatat agagtacttt
     3481 gtacaaaaat atcatttttc tagagaagaa ttaacatctt ttgtggaaaa aacattaaac
     3541 cgtcatggga atcagaaact ctccgatgaa attactcgtg taggaagttc ccctattcga
     3601 aaattaggcc ctaatgatcg tttaattgct cccatagtaa aactagattc atatcattta
     3661 ccttacgatg caggaataaa agtaattgct gcgggatatc attatcggaa tcttgctgat
     3721 ccagaagctg aacaactacg tcgaatgatt gtaaatgatg ggttgaaagc aacgattaaa
     3781 aagatttctc gtttggaagg taagctgctg aatgaagttg tcgctgctta tgagtcccag
     3841 atttagagga ggagaatatg ctaaaagaac aattgtcagg aaatacaatg tttatggatg
     3901 cgatcgattc atggcaagag gcaatccgag taagtgcgcg tcctttgata ggaaagggaa
     3961 taatagagga aagctatgta caagcgatga tacaaaatgt gttggataac ggaaattata
     4021 ttatattact gccactagta gcaatgcctc atgcgcgtcc agaatttggg agcaaagggg
     4081 tcgggatgag ctttttatgt ttagataagc cagtcatgtt cccaagtgaa gaaccagtaa
     4141 aattcttctt taccttttct tctgattcgc caaagggaca tttagactta atggccaatc
     4201 ttggagaatt gctgagtgat gaaaaaatgt atcaaaaatt attcgatgtg aagacagaaa
     4261 aagcattgtt agatttgata aagaagtaaa ggagagaaga aaatggaggt agcaaacaaa
     4321 aaaaaacgag tctccatgcg agctaaagtt caagcgattg gcggtttctt aacaaatatg
     4381 gtattgccaa atataggcgc atttattgca tggggaattg ttactgcatt attcattcca
     4441 actgggtggt tcccaaatga atctctgaat gaactcgtaa gtccgacagt cagctatcta
     4501 ttgccgttac tacttgccta tactggtggt cggatggttg gtggtacacg tggagctgtt
     4561 ttaggtacaa tcggaacaat aggtttgatt attggtgctg acataccaat gttcatggga
     4621 gcaatgatta tggggccact tggtgggtgg atgatgaaaa aaacagatga ttttttagat
     4681 gggaaagttc ctgctggttt tgaaatggtc gtcaataatt tttctatagg aattattggt
     4741 ttcatactga tgtgcctttc ttatctggta gttggaccaa taatccaatc agctaatcaa
     4801 tttgtaacga gtgcaattga agcattagtt tcaactggtt ttttaccgat actgtcctta
     4861 atcaacgaac cggcaaaagt cttattttta aataatgtta ttgaccaagg tgtttattat
     4921 ccattaggta tgcaacaaac attagaggca ggcagatcaa tttatttcat ggtagcatca
     4981 aatcctggcc ctggcttagg actattgtta gcttattctt tatttggtaa aaaggcagca
     5041 aaacagacag caccaggtgc aatcatcatc cattttttcg gcggaataca tgagatttat
     5101 tttccatatg tattaatgaa accactcact atattatcta tgattgctgg tggaatgaca
     5161 ggaatcatca cttttgaatt atttgatgcg ggcctggtag caggtccaag tccaggttct
     5221 attttttctt atttagctct aacgcctcgg ggaaattttt tgggtgtcat ggcgggagtg
     5281 gtcacagcaa cactggtttc cttttttgta acatcagtaa ttttaaaagc agataaatca
     5341 acagatgaag acagaaattt tgaactatca gttcaaaaat cgaaaacgat gaaatcagaa
     5401 ggaagaaaat tattaaaaga agaaaatgct agagaaatat gtaccgagaa aaaagagatg
     5461 aataaaattt cctttgcttg tgatgcagga atgggaagca gcgcaatggg cgcaacaata
     5521 ttcaagaaaa aattgcaaaa agcaggtatt actgatgttg agataaaaaa ttatcgaatt
     5581 gaagaggtac cagaagatag tgatttggtg atcgtacatc aagatttgga ggaacggact
     5641 agaaaagcac atcctaacat gaaaattgtg acattaaaga attatttgca agatccagta
     5701 atcaatggat tagcagaaga aatcattgga ttaaaaacag aagactaatt tatgggaatt
     5761 tttgattaac ctttattaaa attattttgc ctaatataaa aaggttgtga catggcttgt
     5821 gtcgcaacct ttttttgtcg cacaaataat taatcggata ttttatgttc atattttttg
     5881 tgatcaacga gaactgatgg ggcaaagata cattttagat acttttaact gttaatttac
     5941 aaaacaaggt gaaacgaata gaaaaatgaa aaaatcgata cggaagaata tgtttgatta
     6001 aaacatgcat gaagagaagg aggttgatga tacatgaaaa aaatatattc aaaggaaatc
     6061 gattgtttta gaggcattgc tgttttagca gtgatatttt atcatttgcc aaatagtttg
     6121 ctgccaggcg gattgcaagg agtaacgatc ttttttgtgg tttctggttt tttaatggct
     6181 aaaaatacga taagagatga aaaaaacaga caattcagtt ttttacattt ttactataag
     6241 cgtatcaaac gtatttatcc atttttactg ttttctttgg tcgtattcat tttcatacta
     6301 gggattagca acattcgatt gataggaaac attcgtagtg agctacccag tttgattttt
     6361 ggctataaca attggtggca gctgaatcaa ggagtatctt attttgactc ttactttaat
     6421 gcttctttat ttaaacatta ttggtcgctt tcagttgaac tacaattcta tttgatatgg
     6481 ccatttttgt ttattgtcat taagcgtatg agaagaaaac aagtgtttta cttaatctat
     6541 acattgattt ttgtttctat tctttttagt ttgtttttac cttcaactaa ggcatattat
     6601 catacgatag ctaaactttt tccattttta ttaggagttt gggggtattt taatcgaata
     6661 actattggcc gtttctttga acaaaacact tttagtaaaa tctggctttt attactagct
     6721 agtctttgtc tgattctgtt cccgattttt ccttatacac taaatgaact actgatcagt
     6781 atttgttttg ctctattgtt agccagtgtc gatgatatga acattgcaaa aaagaccaaa
     6841 atcgctagta caaaactttc ttttgtagga aaaatcagtt atgaacttta tttggtgcat
     6901 ttcccagtat tgattgctct gacaaatcta ttcaaacatc accattggaa atatttggat
     6961 atcgcaggtc tattcactgt gtttttatgt acattactca tgattacttg ctttagaaaa
     7021 ggagtgcaaa agatgagatc acccaaaatt tggacagcta tggcactaat gataatcgga
     7081 gtttctgtac tggtattacg tgctcctgcc agtcgactga ccgctgacca aaatcagttg
     7141 caaagtttat taaaagaaaa ctctagtaaa gcaaatgcag gagaaaatct gttgtcacaa
     7201 gagaagaaaa tattatttat cggtgattct gttatgctgg gggcgtatca ggaactaaag
     7261 gagacattta aaaaagaagc attggtagat gcaaaagaat caagacaaat cactgcgtta
     7321 ccagaaatct taaaggagca taaagattta gtttcttacc aacaaatagt gatcgggtta
     7381 ggaaccaatg gtgtgataac agatgaagct atagaacaaa caatgtcttt gctgaaagat
     7441 aaaaaagtat actgggtaaa tataaaagca ccaactggct ggcaagatac gataaatgcc
     7501 acattagcta cattgccaaa tcagtattct aatataacga tgatcgattg gtatagtgag
     7561 agcaaaaatc atcctgaatt tttttatgat gatgaaaccc atttgaatga aagtggacgt
     7621 acagcttatg caaagtatat tgcttcagtt attcaataag gtagtgatta cgtgtgttaa
     7681 ccttactttt tcctaatagg tgaatcaatt taaataaaaa atacttcata tgataaacgg
     7741 tgtttaagag gaggagcttt ttaatattcg tggtaaaaaa gagatgggcg aggtcgttaa
     7801 ctgagagctt cttggaaaga aatcgctttg gatttatcct ctttgttata cagaacatga
     7861 gcgagacgac aatactagga tgtatgttct gatcgaagtt ttatggttac aagcagcaat
     7921 cagcttagat aaaattaagg atcgtaggaa ccaagtaaag aaggctgtta gtaaagaaac
     7981 aaacgatcaa gtatgtttta ctcttaagaa gatgcagtga ataaataata gtaatataat
     8041 gctagaaggg actgtgacaa aagacgtgtc acagcctttt ttgttatttt aattatcgtc
     8101 cagtcttttt ttataaatga ttgcttaagc attaaatatt aaaaaatata gagttcaaaa
     8161 ttactgcaca attagatgaa aaatttgaac aacaaaaagt gtatttttat actataatag
     8221 cttttgaatt tagttactct attcaaaagt tatgctatgg acaagaaaaa gaataaatag
     8281 aggtgagaaa atgaatagtt tcaagaaaaa atttgtgagt agatttttaa atatgaaaaa
     8341 tttttttgaa aaaaagggag attatgatgc atataattat gtattaaatc tagaaaagca
     8401 aacatattat acactacgta aaaacgatga taatgacttt tggactatgt tacctaaaat
     8461 gctttcgatt gactctaagc tttccctttt agaggaattt attaatttag acattgaaga
     8521 attttgcggg ggaaaagagt taattaatat ggtagagagt gattatcata cctattataa
     8581 agaaagttgt ggatacaaag tggatgagca atgtccacaa tcaattattt tttacattga
     8641 gtaatatgaa ctattaagca ggaaagcggg agcgtcaata attttgtgta aataaattgt
     8701 cctcctgcaa aataattagt ta
//
LOCUS       DAPBAB010000090         8691 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPBAB010000090 DAPBAB010000000
VERSION     DAPBAB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.095"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(83..641)
                     /locus_tag="RD444_002571"
                     /pseudo
     CDS             complement(83..641)
                     /locus_tag="RD444_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            810..2921
                     /locus_tag="RD444_002572"
     CDS             810..2921
                     /locus_tag="RD444_002572"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549893.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            2899..3549
                     /locus_tag="RD444_002573"
     CDS             2899..3549
                     /locus_tag="RD444_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDT7549894.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3536..3988
                     /locus_tag="RD444_002574"
     CDS             3536..3988
                     /locus_tag="RD444_002574"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549895.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4005..4310
                     /locus_tag="RD444_002575"
     CDS             4005..4310
                     /locus_tag="RD444_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HDT7549896.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4322..5599
                     /locus_tag="RD444_002576"
     CDS             4322..5599
                     /locus_tag="RD444_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HDT7549897.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5618..6766
                     /gene="dgoD"
                     /locus_tag="RD444_002577"
     CDS             5618..6766
                     /gene="dgoD"
                     /locus_tag="RD444_002577"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDT7549898.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            6778..7680
                     /locus_tag="RD444_002578"
     CDS             6778..7680
                     /locus_tag="RD444_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HDT7549899.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8354..>8691)
                     /locus_tag="RD444_002579"
     CDS             complement(8354..>8691)
                     /locus_tag="RD444_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7549900.1"
                     /translation="DYLLSIDNQLRQSYEVYQELLAAFDAKDFTEFFNLIESLPHSLN
                     DGFKKAICYLKKHTEAITNSLKYPYSNGKLEGKNNLIKVIKRIAFGFRTFRHLRMRVL
                     IQQNICEII"
BASE COUNT         2835 a         1324 c         1776 g         2756 t
ORIGIN      
        1 taaaaaaata tttggcgtca ctactcagtt tgagtaacaa aaccaaatat tatagaaccg
       61 gctttttcag ttttttccct tcacaaacaa catcgatgat tttctcggca tgggtacggg
      121 tatcattcat taattctgta tcggttggat atttgatgtt ttgtggagca caagtggcat
      181 ccaaaatcaa tgttccggaa ttgaaatcgt cataagaatc gttgtcctta tcgtcttttt
      241 tcttgtcgaa gacgagtatt ttttcattga tttctatcaa cgttgtttcg tccaagcgtt
      301 tccggaaata gaccattgta gagcatcgaa aggcttggca tcttgatagc ccggcaaacc
      361 gatgaagaac tataaatacg ggttttcttg aatttgtaag accgtttctt cgtcgctata
      421 gctatattcc ttttgaatca gtagagctcc gaaggtcatg cgaaagggtt ttgcgacgtt
      481 tccttttttt tacgaaaatt tttagcgtag tctttttcaa gttcagccca tggaatgagc
      541 tctgcttttt gaatccaacg gttagacgga ttcattttga gacctatcgg ttgattgaaa
      601 tcttcaaaag ccaattgaac ggttgtttgt aatttataca tgaaaagcct ccaaaattga
      661 gtggtactcc acaccattct gatggtgcat aagataaaaa attaaagaag tgcctgtcat
      721 ttgtctgatt taagccaagg tttgacaaat tttggatatt ccaattaata ctgtaagcgg
      781 ttatatttaa ctcagggagg tagatgaatg tgaatacaaa agatttaaaa ctgttagaaa
      841 ccattgtacg atcacatgtg acaaactctc aacaattgaa atcattagta catttttcga
      901 ctaggcagat aacttactct ataaaaaaaa ttaatgatga attatccctt catggattga
      961 gtctgataaa gcgaagtaac tcaggggagt ggttatatga agccaatgat cttcaaaaac
     1021 ttttgatata catgaatcaa gcaatggttt ttaaagaagg tgcgattcct gaatatataa
     1081 acgatgattt ggcttatagt gagataagaa gatattcgga actgttattt attctagcca
     1141 accccaaaaa agtgacaacc caagaaataa tggcgtttat tggtatcagt aaaaatacgg
     1201 ctagaaacga cttttctaaa ataaaaaaag agttgccacc agactacact ttgatatttg
     1261 atccccaaaa tggctatatg attgaatcaa gcatggcaaa gcttcataca ttgctcttag
     1321 aagttttcaa ttatttattt gaaaggcatg caacagttaa ccgttttgag tcactctttt
     1381 ctgaagaaag ggaatttgtt ggttttttta ttcacaccta cgaacaaact tttaaattga
     1441 aattttctga taattcacta accaaacttt attatttgat aggtttatca ctttctagtt
     1501 ttaaaagaag gacgaaagga cagattgatt ttagttatga tttacaggaa ttagatgagt
     1561 ttcaaatgat tcgaagctgc attgatcaac attatcaaca tgctgagata gctatagatt
     1621 atgaatggtt gacgcttctg tttttatcat tcaatacaat ttcaaatgag tttgaaattt
     1681 tagattcaag aatctatcaa gtaattatgg agatgattac cttatttcaa agtaaatcag
     1741 ctattcggat tagtaataaa gaagagttga ccaatcgatt gatgaatcat ttacgacctg
     1801 caatctatcg aattcgctac gaattaccat ttgaaataga tgatttagct gaaattgtta
     1861 aggaaaacca tgaagttagt gcggtactta attttgttaa agaatccatt gtacctttgg
     1921 aagcattttt gcgtaagacg attcctgaaa ctgaattata tttgatttct gtttattttt
     1981 gttcggagct attaaattct tctattatcg aaacacctgt tagaaaaaag gcaatcgtcg
     2041 tatgtagcaa tggattgatt atggccaatg taatggcgaa aacattgaaa gaaatttttc
     2101 cagatattca gtttgttgct agttcttcgg tcagagagat ggataagttt gtcgaagatg
     2161 ttgatttgat ttttagcaat caaccattga aaagtgaaat tccattatat attatacaac
     2221 cgattatgaa cgaagaagaa aaaaaccgct tgcggaaccg tgtactcatt gatttttcta
     2281 cgaataatat cagcaaagag actaggaacc ttactgaaat tattaagcgt tactctgctg
     2341 ttcaaaatga agaaagtcta caaactgata ttgagagatt tttaattaat tatgcaaatt
     2401 cactaatata tctagatcgt caaagtaaac aggcacaaaa gatatctttt gtggaattgc
     2461 ttgtttctga agggattatt cttgtatcta cgaaacaact tacttggcag aacgcgatgg
     2521 aattgtcttg tcagccttta ttaaaggagc aggtaattaa tcagaactat cagactattt
     2581 taatgagtca aatggcggat gaagaggctt attattttct taataatcgt attgctattc
     2641 cacattcatc gccagaaaat ggggtaatta agggcggttg ctcgttactt atatcagttg
     2701 atccaattat ttttcctgga aataggccta tccattttat ttcccctttt gcaattggaa
     2761 agaaggaaaa ttatttactt gcgattaacc aattactaca actttctaag gacgaggaat
     2821 atcagcaaga aatacttctt tgcacgacgg aagatgaagt gagaaaaaaa ataaaaaatt
     2881 ttttaaggag gaagcaatat gtcaataacg aattttcctg aattaaccat cattttaaga
     2941 ggatatagtt ttgaagaggc gatgtttgtc atggaagaat tggctgcgat gaacacttcg
     3001 gtaggaattg aagtaacaac caataatgat gactttgcac gtatcatcac agaaggcaac
     3061 aaaaaatttg ggcaacaatt attgattggt gcagggacag ttttaacatt ttcacaagca
     3121 gatcaagcaa ttgctggtgg tgcgaaattt ttattaggac caaaaaaatt ttcaacacca
     3181 attttttcgt tggctaaaga acgtggggtg gtaactgtgc catcagcttt tacgccatcg
     3241 gaaattcaag ttatgtttga tgaaggtgcc gatattgtaa aggtttttcc agcagtcact
     3301 gtagggccaa cctactttaa acaactggcc ggtccttttg gtatacggcc tttaatggca
     3361 gttggaggag tgacgattga taatattgcg gcttttaaaa aagcaggagt tgtgtacttt
     3421 ggggtaggat caagcatgtt tcaaaaagct gatatacaaa accgcaatgg gcaagctatt
     3481 cgaaaatcaa ttcaatcata tttagataaa ctagctgaag caggaagtga agaagatgag
     3541 tctcattaaa ttagaagaaa aaaatatttt gttaaatcaa agtttttcaa accaagaaga
     3601 atactttcaa aagattacaa agaaactgat gacggaaaac atggtaaagg agggatatct
     3661 cgagtcaatc actaagagag aaacaactta tccaacgggg ctatatattg gggaacgctc
     3721 aatcgcaatt ccacatacag attatcagtt atcaaatgtt accgctattt tagtatccgt
     3781 tcttaaagat agtttgcatt ttcagcgaat ggatgatccc gaagaaatgg tttcagtgaa
     3841 tgttattatt caccttttat tcgatagtcc cgataagcaa ccgcaattat tgaaggagtt
     3901 gatgagtaaa gttaccaatc aggatttctt acaagcaatt atggaagcaa agagtaaaac
     3961 agaagtttta actgcattta atcaataaat tggagggaaa tacaatgagt aaaaaaacaa
     4021 ttatcgtcgc atgtgcatca tcaatgatta catctacaat ggcagctaat aaaattcgga
     4081 aaatagcaaa aacaaatgga ttaccagaac ctcgaatttt gcaactaaaa ttttcagaag
     4141 ttgcaggtgc tttagaatca aatcaagttg actttatcgt tcctacaggg aaattacaag
     4201 aaaatgtaac caaaggaatt aaagttatac ctggaacacc atttgttact ggagtgaatg
     4261 aagaaaaatc tgaatctttg gtgttagaag cattgaaaga gcttgactag gagggtgaaa
     4321 aatgaatatt gtcattgatg cgttaaactg gatctcaagt ttgggtccga tgattatgat
     4381 gcctatcatt atcttgattt taggattatt ttttagaatt aagctaaata cattactaaa
     4441 atcagcgttg actgtgggga ttggttttgc gggtgtaaat attattatta attggtttat
     4501 ttcacaggta ggtccatctg ttcagttaat ggtcaaaaat tggggtattc aaacaagtat
     4561 tatggatgtc ggctggccag ctcgtgcagc agccacttgg tcttttcctc tagcagcagt
     4621 tatggtattt attattttgg gtattaatgt gttgatgctg atttttaaaa agaccaatac
     4681 agtaatggtc gatttctgga gttataatca ctatattttt gtagctgctt tggtttatta
     4741 tgcaacggat aaaaatatta tattggcggt aatcttcggg gcgattgatg caattattag
     4801 ttttaaactc gctgattgga caacgccgtt agtagaggat tactttgaac tagaaggggt
     4861 taatttcccg actgcgaact cagttggttg ggcacccttg gcttggctac ttaacaaatt
     4921 gtgggaccgc attcctggat taaataaggt agatgctcgt cctgaaaaaa ttcaagagcg
     4981 gtttggtttt gttggtgagc ctatgtttat gggatttgtc atcggcttgg ttattgggtt
     5041 attagcaaaa gaacctgttg ataaggtatt gatagtggga atgagtactt ctgcaacaat
     5101 ggttctaaca cctaaaatga tgcaaatttt aatggaagga ttattaccct ttgctaattc
     5161 tgttaagaaa ttcttgaatg taaaatttcc aggaaacaat tttacaatgg gtgtggatgc
     5221 tgcgttgaca gttgctaatt caagtgccat tgctacgggt attatcatgg ttccgattac
     5281 cttagtgttg gctgctattt taccaggtaa caaactctta cctatttcag atattgccta
     5341 ccaagcaatg tggctatctg catggccggt cgccttttca aaaggaaata ttattcgcgg
     5401 aatactttcc accacgatta taacagcttg tgtgctttta attgctacta gtttagccgg
     5461 catacataca caattagctt tggctggggg attccaatta ccagcaggaa tgacacaaat
     5521 atctaccgaa gatgcaggta cacacattat tggtttcatt ttaatgaaga ttgctgagtt
     5581 tatttcaaat attttctaat attcaggagg gcatacagtg aaaatttcaa aaattacagt
     5641 ctataaagta aaaccaagat ggatttttgt caaaatatct acagatgaag gtattgaagg
     5701 ctggggtgaa atgatttctg gaactaagac tgaaacagtt gtggcaggag cttatgagat
     5761 aggaaatcga ttcttagttg gtcaaaaccc atttgcaatt gaacgtctgt ttcaggaaat
     5821 gcatcgttca tttttccgag gtggtcctat taatggaacg attgtctctg gacttgaaat
     5881 ggctttatgg gatattaaag ggaaagcctt aaatgttcca gtttatgaat tactaggtgg
     5941 agcagcccgt gataaaatca aagtatattc ttggattggt ggagatcgtc cttctgaagt
     6001 agtagagcag gcacaagatc gagtagatcg tgggtttacc gccgtgaaaa tgaacgcgac
     6061 agaagaatta cactacattg attcttatga aaaagtagct gaagtagtgg aacgtgttgc
     6121 ttcaattaga gaacgttttg gagatacatt gagcattggt gtcgattttc atggtcgtgt
     6181 tcacaaacca atggctaagg ttttagcaaa agcactggaa ccctatcatc caatgttctt
     6241 agaagaagtg gttttgccgg agaatgaaga acatttcagt gaagtagcca atgcagttgc
     6301 tgtaccacta gcaacgggtg aacgtttgta tacaagatgg caatttaaat caattcttca
     6361 acagggagcg attgatatca ttcaaccaga tgtagcttta tgtggaggga ttttagaaac
     6421 aagaaaaatt gctgcaatgg ctgaagctta tgatatagca gttgcccccc atgcaccata
     6481 tggaccgatt gcgttagcag ctacattaca agttgattct tgtacaccta atgtgtttat
     6541 tcaagaacaa agcttaggta ttcattacaa caaagggttt gatttgttag attttgtaaa
     6601 gaataaagag atattccaat acaaagatgg cttcgtagat cttccgacaa aaccaggttt
     6661 agggttagaa atggacgaag atcgagtgaa agaaattgca caagaaggtt tagtttggac
     6721 caatcctaag tggaaaaatt atgatggtac aatcgctgaa tggtaggaga tgagtgcatg
     6781 atcaatacgg ttacaggccc aatcgaagct gagtctttgg gtacaacatt tattcatgaa
     6841 catttatatg tcattcctaa tgagttaaga aagtatgaag attatacctt ggatgatatt
     6901 tatcatagta ttgcggaagt tatggctttt aaacaagctg gtggaaaaac tatcgttgat
     6961 ttgacaccaa ttaattatgg acgaaatccg ttagctttga aaatgatatc tgaagctacc
     7021 ggagtgaatg tagcatttgt tacgggattc cataaggaag agtttcagcc aaaatggttg
     7081 acaacgctca atgatgatga gatctttgag ttgctttatt ccgaaataac tcacggagtc
     7141 acttcacaaa accttttgcc aggagcaatg aaagtaggta cgagtaaaaa tgaggttaca
     7201 ttggctgaac aacgaatact agccatagcc ggtcaagtac aaaaaaaaac agggattccc
     7261 attattacgc attgtgatgg ggggacgatg ggactggagc aaattaattg tttaacaaaa
     7321 aacggtgcga aagcaagtaa tatttgtttg agtcatgtag atttaactaa agattttcac
     7381 tatttagctc aaatctgtga aacgggagca agtatttgta tggatcatat tggccgagag
     7441 ttggcgaatc atgatgcaga ccgcgtccaa ctaattaaac gactggttga tgcgggttat
     7501 gaaaatcaga tttgtctttc aggtgatatg ggacgaaaaa aatatttgcc aacttatgga
     7561 ggaacgcctg ggcttaccta tattttaaca agtttgaaag aagaactatt aaaagtaatg
     7621 agtgaaccaa cttttaataa aatgattgag atgaatccaa ttaatatact gagtaagtga
     7681 agtcaattga atcattcaga agaactaatg aaattagttg ctttcgcagg gttaattctt
     7741 ctctctgggt ttggaaaaga gggcttagct tcggattccc tagttaaact aggggtttta
     7801 tcaggttatt gtactgatta taagtacctg aattattcat gaaatacttt ttccgtcgtt
     7861 ttataggcag ttgggtcaat aaaaagatca gaaaaacgaa cgagagactc tcataacgtg
     7921 ccactaaata aaaatcgtac gttattttaa aaaatgggtt gattctaggc agtactttgt
     7981 cggtctattt ttagaaaaag aggttctttg tcaaacatac atgaattcgc aagctcattc
     8041 accctgaagg atgaaaggca gaaactgtag tatactaatg gtggaaagga aaaaaagccg
     8101 ccagcaattt ggaaaagaac gttatgtcga ttgtcaaact ggcttgcttt atttctcgag
     8161 aaccctgaaa tatagccaat gagcatgtgt ataaaaaatt gattttatat gaaataaagc
     8221 actcctttcc taaaattatt ggaaaggagt gcgtttttta tggaaattat ttaggttctt
     8281 tgtcaattgc tattgttttg ctcaaatcgt atgttaggaa atgcttcggc atttcctttc
     8341 cttttttgcg ttattaaatg atttcacaga tgttctgttg aatcaggaca cgcatccgga
     8401 gatgacgaaa agtccgaaat ccaaaagcaa ttcttttgat tactttgatg aggttattct
     8461 ttccctccaa ttttccgttt gaatagggat attttaagga gtttgtgatt gcttctgtgt
     8521 gctttttcag ataacaaatt gcctttttaa acccatcatt caaggaatgt ggcaatgatt
     8581 caatgagatt gaaaaattca gtaaaatctt ttgcatcaaa agcagctaat aactcttgat
     8641 agacttcata gctttgtcgt agttggttat ctatagagag taggtaatca a
//
LOCUS       DAPBAB010000091         8659 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPBAB010000091 DAPBAB010000000
VERSION     DAPBAB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.096"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            194..2236
                     /locus_tag="RD444_002580"
     CDS             194..2236
                     /locus_tag="RD444_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549901.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2247..2867
                     /locus_tag="RD444_002581"
     CDS             2247..2867
                     /locus_tag="RD444_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDT7549902.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2879..3337
                     /locus_tag="RD444_002582"
     CDS             2879..3337
                     /locus_tag="RD444_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549903.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3356..3634
                     /locus_tag="RD444_002583"
     CDS             3356..3634
                     /locus_tag="RD444_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549904.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3659..5026
                     /locus_tag="RD444_002584"
     CDS             3659..5026
                     /locus_tag="RD444_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDT7549905.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5041..6186
                     /gene="dgoD"
                     /locus_tag="RD444_002585"
     CDS             5041..6186
                     /gene="dgoD"
                     /locus_tag="RD444_002585"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDT7549906.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6213..6704
                     /locus_tag="RD444_002586"
     CDS             6213..6704
                     /locus_tag="RD444_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549907.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7147..8346
                     /locus_tag="RD444_002587"
     CDS             7147..8346
                     /locus_tag="RD444_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7549908.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT         2688 a         1417 c         1845 g         2709 t
ORIGIN      
        1 ccgaatattt aatattcaca ttagccctaa aataattgtc ataccatgtt acatttgata
       61 taacagcgtt cattttaaaa atagctgtta gtgtgacaaa acattaaacc aagtgtgaca
      121 aaaaacgtga tgaaagcttt ccggaaacgt atgtgaacaa gtatataatg attttgtcag
      181 gaggatagtc aaaatgaata aacgaattcg tgaaatactc aaactgatta ttaaaaaccc
      241 tgaaatgaaa ctttccactt taacaagtga acttgatttg acacgtcgtc aaatcaatta
      301 cgcgatcaat caatttaatg aagaattgaa aacaaagaac attccgttaa ttcagagaag
      361 tcattctgga aatattacgg taccaatcga ggttatccaa atgatatcac agcctgatta
      421 cgacataggt gatgatcagg aacttgtcgt tctcactgat agtgaaagac aagcattgat
      481 agtgatgact ctgatcgcta atatcgatta catcagtctg gatcatttgg tagatgcggt
      541 tgatgttagt aagacgactg ttatggaaga tatcaaacga acggaaccgt tcatcagaaa
      601 ttatagtctg acaattcgat ataatcggat gaatggttac caattggttg gttcggaaca
      661 ccgtatctta cagctgttat cggatctagt aaaacggttt ccgatttttc gccgagaaat
      721 gattcgcaga aaattggcac aagatgtttc tgaagaagaa atcgtccatt tgatccataa
      781 catggaacaa atgttgcatc ttagttattc tgatgagtcg gtagactatt tgcagacatc
      841 agcgagattt ttagtaagcc gtgggatcaa gatgtcagat aagcacgcgt tttttcccga
      901 tgatgtgacg gatactcctg aattccgaat gctgatgatc ttggtacatg aaacaggttg
      961 gagtctatcc aaaagttata tggagtggtt cacgttatta tttttaactt ccaatatttt
     1021 tgagagcaaa actactcaaa attatgattc ggatcaagaa ttgcgaaaat tgatcagcca
     1081 aatggttgaa aatttccaaa atcaaacgtt gattttgatc gatgaccgag aagactttga
     1141 acgtaggata ctgagtcatc tgcgtccagc ctgttttcga atacgctata acttgagttt
     1201 aggagtttat tcaatagata gtctgatcaa ggatagtaat catgcgattt tgattgattt
     1261 gatgaaagaa ttgattattc ctattgagaa ttggctaaat aaggcctttc caaatgatga
     1321 attagatctt ttatcctatt actttggcta tcaattaagt aatcataata aattgaataa
     1381 gcaacgacaa agggctgtag ttgtttgtac taatggtgtc atggtctcaa agttagtacg
     1441 agccaatatg gaaaaacttt ttccagagat ccatttttta gcttccctat ctgtgcgtga
     1501 tttctacaaa tttgaagctg attatgatct ggtctttact acgacgccac taaaaagttc
     1561 tatgctgcag tttatgatcg agccaatcat gacatatcag caacaaatca gtttacgata
     1621 tcgagttttg agtgatttag gaattactaa ggttgatcga gcaatggatg aattgttaac
     1681 aatcattagg aaatatgcaa atatcaccaa tccagtttcg ttgcgagaag aactgcagta
     1741 ctttttgatc aaggaagatg gaagcacgcc gctggacaac ttcaatgtat tgccttcatt
     1801 gacttattac ttgcggccga gttatgtaca aatcgttgaa gaagagatgt cttgggaacg
     1861 agcagtggag gtggcttgcc gaccgttatt ggaacatcaa atcattgact cgcgttttat
     1921 cgctgattgc ttggatcaaa tcagacaacc gggatatgca ggttacttgg gaatgaagac
     1981 ttgcattccc catacaacgg tcgataaagg tgtaatcagt gacggtgtca gcattttagt
     2041 ttgtaaaaag ccgatacgat ttcctaatgg tgaagatgtc agcttgattt tgccgctttc
     2101 attttttgat ttaacgaagc atctgcgagc catcaatcaa atcgctgata tcgccaaaga
     2161 tgattcattg attgagcagt tattgattgc tgatgacgaa aagacagttt atcaactatt
     2221 aagacaaaga tcttaaggag gaaaacatga gactagcaga ttatcctaaa ttgacagtga
     2281 tcatgcgtgg ttatactttt gatcaggcag atgctatttt acaagcaatg caggaatttg
     2341 accatcaatt tgccgtagag atgacaatga acacaaaagg agctcttgaa aatatacatg
     2401 aactaaatca acgttatggt gacacgactt atatcggcgc tgggacggta aggacattag
     2461 aacaagccca agcctgctat gaggctggtg caaaattttt attaggacca catatgttca
     2521 caaaagaaat gttgtcttac gccaataaga aaggattatt ggcggtacct gccgcaatga
     2581 ctccttcaga agtcgaccgt atgtttaaag aaggagctga tattgtaaaa gtatttcctg
     2641 cagcggttgt tactccacgt tttttcaagg atattcaagc acctttaggt aagttaccac
     2701 taatgggggt tggtggaatc agtaaggaga atgcgaaaga gttttttgaa caaggtgcta
     2761 gctatttagg tcttggttcc ggtatgttca acaagcaaga catcgaagag ctaaatgtta
     2821 aaaacttagc acgttcgatg aaagaattaa ttgaggctat taaataggag tgaggacttt
     2881 ggaactttat ttgaatgagg aactcgtttt tcgaaatgtt caagcaaaaa cagatagtga
     2941 agtcttagcg tttttagcaa gcgagatgta caaaaaaaac tacgtgaagg aggaatatat
     3001 tcaagcaatc caagaacggg aaaaagaata ccctactgga ttaccatcaa caccaccagg
     3061 aatcgcaatt ccccatgcaa actatgaaat ggtcaataaa acaactttag caattgcgac
     3121 tcttaaagat ccggtcctat ttcataatat ggaagataac aatgagcagt taccaatcca
     3181 aattgtgatc atgatggcga ttggggaacc acatgggcaa gttgaaatgt tacaaaaaat
     3241 tgttggaatc atacaagatg agccactgcg gcaagaaatg atcagagcag gcaatgatac
     3301 tgaattatta gaactattaa agaaagcggt tttttaatat aaggaggaaa tcataatgaa
     3361 acgaatttta gtagcatgcg gaaatggtat cgccacttca acagtagtag caacaaaagt
     3421 tagagagtat ttgcaacaac atggcattga tgtttcaacg actcaaacaa aattgatgga
     3481 agttccagga aaagttcaag actatgatct attggtgacg actggtcaat ttgatggtca
     3541 aacaggtggt gtaccagtaa tcaaagggat gccgatttta acaggtatcg gtgccgatca
     3601 gacaatggaa gagattttga acttactgaa ataatagctg atcaaaggag ctgaaaaaat
     3661 gaaaatcatt atgaacgtct ttaacttctt tattgatgct ggaccaacag ttatgttgcc
     3721 agtcatcatc acgattattg gtttgatttt tgggttaaag atttctcgtg cttttaaatc
     3781 aggtttgaca ttaggtatcg gttttgccgg gatcaagttg atattggact ttatgacgac
     3841 caatgttgga ccagctgcca aggcgatggt tgatcgaact ggtgttaaac tagatgcttt
     3901 ggatgttggc tggggttcga ttgcggctgt tacttgggca tcgccaatca ttccaatctt
     3961 gatttttgcc attctattgg tcaatatcgt tttgctgatt ttgaaacgta cacatacgct
     4021 ggatgttgat atctggaact atcaccatat ggcaatcgtt ggtgttatag tgtattttgt
     4081 gactaagaat gtcttcttag gtgtaggtgc atcagtagtg atggcaattg cgacttttaa
     4141 gatttcagac tggtcgcaac cgatggtaga aagtttcttc ggaatccctg gtgtttcttt
     4201 accaactgtt tctgcgcttt catcactggt tattgcttgg ccgttaaatt ggctgctgga
     4261 tcgtatcccg ttattcagaa aatcaaaatt caccatcaaa gatgcacaaa aatatttggg
     4321 attttttggt gattctatga tcatggggtt agttattggg atggtgattg gtgttttagc
     4381 cggctatgat atcaaagcgg ttttacaatt aggtgtcagc atgtcagctg ttttggtttt
     4441 gattcctaag atgacttcat tatttatgga aggattgatg ccaatttcag atgctgcaca
     4501 aaaatggtca caaaaaaaat tcaaaggtac acgtttattt atcggattgg atgctgcagt
     4561 tgttgtaggt aatccagatg ttatcacaac agcattgatc attattccac taacgattgc
     4621 attagcttta gtattgccag gaaaccgtgt gttaccattc gcagatttag cggttgttcc
     4681 tttccgcgtg gcaatggtcg ttgccttgac tcgtggtaat ttattaaaaa atatcattat
     4741 tggcttagtt gtgacagctt cattgctatg gtgtgggtca gctactgcgc caatgctgac
     4801 agcaattgcc aaatcggttg gtattaactt aggaacttct tcaatgttaa tctcatcatt
     4861 tgctgcgact tccatgatcc aaagttattt ggtatttatc gcatttgctt acaaaccaat
     4921 catcggtatt ccagtgttgt tattagcctt tgcgggcgca tggtattact tcgatggtat
     4981 caaaaaagtt aatcatgaaa tggatgaacc attgaaggca caatagtagg agggtttaaa
     5041 ttgaaaattt cagatgtcac agtgtataaa gtaaaaccaa gatggatttt tgttaagatc
     5101 tcgactgacg aaggaatcga aggctgggga gaaatgatct caggaactaa aacagaaacg
     5161 gttgttgctg gagcttatga gataggaaac cggctgattg gacgtaatcc gtttgaaatc
     5221 gagcgcctct tccaagaaat gcatcgatct ttcttccgag gcggtcctat taatggaacc
     5281 atcgtttcgg gtttagaaat ggctctgtgg gatatcaaag gaaaagcgtt taatgttccg
     5341 gtttatgaat tattgggcgg tgcagcacgt gataaaatca aggtttattc ttggattggt
     5401 ggagatcgac caagtgaagt tgcagaacaa gcacaagatc ggtttgatcg gggctttacg
     5461 gccgtcaaga tgaacgcgac agaagagctg cattatatcg attcctacca aaaagttgat
     5521 gaagtggtgg aacgagttgc gtctattcgc gaacgttttg gtgatgcaat ggatattggt
     5581 gttgattttc atggacgggt ccataaaccg atggcaaaag tgctagcaaa agctttggag
     5641 ccattccatc caatgttttt ggaagaagtc gttttacctg aaaatgaaga acactttaaa
     5701 gaagtagctg attctgtggc tgtaccatta gctactggtg aacgcttgta cactcgttgg
     5761 caattcaaga acctctttaa acaaggtact gttgatatta tccaaccaga tgtggctctt
     5821 tgtgggggaa tcttggaaac ccgtaaaatc gcagcgatgg cggaagctta cgacatggct
     5881 gttgcgcctc atgcgccata tggtcctgtt gcactagcag caactctgca agtagactca
     5941 tgtacgccaa acgtctttat ccaagaacaa agcttaggga tccactataa caaaggtttt
     6001 gatctgttag attttgtgaa gaacaaagaa gttttccaat ataaagacgg ctacgtagat
     6061 ctaccttcaa aaccaggtct gggcttggaa atggatgaag acaaaatcaa agaaattgca
     6121 caagaaggtt tgatttggac caatccgcaa tggaaaaatt atgacggtac gatcgctgag
     6181 tggtgataaa tgaaaataaa ggatgatgaa agatggcaga agcacttatg atcacagttt
     6241 ttttaggact gctgattatt ttgcaaataa tcgatcaaaa gaaaattatc gtgtttgctc
     6301 agagtagtcc tgtaaggaag agtttgagta ttttactgac agtcgccatc ctgttgattt
     6361 tctggccgga acatctgact gatcagatta aactggttgc ttttgcttct gtcattttga
     6421 tcaatggttt tttgaaggag ggtctagcaa aagattctgt tgttagattt ggagtattga
     6481 acggtaactt taatcgatat cctcaagtcc aagtagaaaa actaggtgac gataaatcat
     6541 ttatttcatt ttacaaaagc aagaataatt ctttttcaat gatttttgat aaagatcaaa
     6601 ctgaaatgat ggatgtcttt caagaacttg atatccattc caaaattact ttagatcaaa
     6661 gttcggaaga aaaaagagca gttgtaaaaa aagataatga atgattctac taccgaaatt
     6721 atgatttctt tatcacgaat aatgaaactt gttttaatga atcatattga ttgaagtata
     6781 caatgtatat taagttttgg aaagaaatat attggtacaa gatatagcag gaatcgatta
     6841 ctcgatccct gcttttttta acttcctttg cagcaaaaaa agctcgtaat ctaagagatg
     6901 atggatagtt tttagaatat atatattttc ttcaattcca gaaaaaaatg accaagctag
     6961 tatgctaaaa aagtacgatg cttctatgat cttttttccg tctttattaa cagatgtttt
     7021 aattgatcaa cgcatattgt aatctaccct aatttccata gttcttcacc acttttaaaa
     7081 atagtaaaac ccctacaagt atgctatagt aaattgctaa agaaaaacca ataaggtggg
     7141 agatgtgtgc gcgatcaaac aagcggaaaa atgattcgtt tttattatac aagtttgcat
     7201 tttctttatt ggatgatgtt ttgttctgtc tatgggtttg cgacattgtt tttgatttat
     7261 ttcaagattg atccagctaa aatcggaggg atcttagcat tggtcaatat tgtttcaacg
     7321 gtgcttcagc cttttgtcag tcaagtgatc atcaagaaaa gaaagatccc actgatcacc
     7381 gtgctgaaga cgatgatagc aggattagga gtgattctct tagctggggt attgattccg
     7441 aatttgccga tcattactta tatcttgggt gggattttag tagtttctat gcaatcattt
     7501 ctcaatgcgc tggcatttga gtattcaaat accggctaca ggatcaactt tggcttttca
     7561 agagctggtg gttcattttc ttatgctgta acttcttttt tactgggaca gctgttagcg
     7621 atttattctg ccgcgatttt gccaatcttg gttatagttg aagcagtgat atttttcgtt
     7681 attttgcatc tgctgccgga tgtaaaaaat caagaagttt ttcctatgac aaaagaacag
     7741 gatcgctcag tttctttgag aaaaaaatat cctttcctga ttttgttatt tttaggtttc
     7801 agttttcttt ttactttcca caccatgatc aacagctttt tggcacagat ctttgaaaat
     7861 atccacggcg gcagtaaaga agttggcatc gcattgatga tcgccgcttt atgcgaatta
     7921 ccgggaatga tcttttttga acagcttgtc aaaaagaaat ccagcaaatt ttggctgtct
     7981 gttgccagta tttcatttgc tgtcagagga ttcatgatgc tgctggccag ttcgatcctg
     8041 atgatggaaa tgacgcatct cctacaagcc ttcagtttcg ctttgtttat tccagcttcg
     8101 gcttatttgt tcaatcaatt tctggcggat gatgatcgtg tatttggaca aacgatgatc
     8161 atcatcgcca caactcttgg tggggtgata ggaaatgtct tgggcggcag tctgctgcag
     8221 catttcggtg tatgggagat gctgattttc ggtactggtt ttgcggtgct aggtgcttta
     8281 ttaactattt tgggcattcg aaaaataccg aagacaggaa acgatagtga ttctcttgtt
     8341 gagtagcata aaaatactta gatactagtt ttatataaat aacatatagt gattattggc
     8401 gtaattacaa aaaacgagaa taacttaagt aagcatttct aaataattga gaaacctcat
     8461 ctaaaaagat aagaaaatat ccctaataaa acttctgtaa cattttagat aactgattgt
     8521 aatctgaata ataaactata aggagacggt tagacggtct cctttttttg cgtattataa
     8581 ttctagaatt gttggaaatt gataataatt aacaagaaat attagacagg aacttcgaac
     8641 tgcttatcac attcttatt
//
LOCUS       DAPBAB010000092         8400 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPBAB010000092 DAPBAB010000000
VERSION     DAPBAB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.097"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..1260
                     /locus_tag="RD444_002588"
     CDS             <1..1260
                     /locus_tag="RD444_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDT7549909.1"
                     /translation="SVECIERNLDPMTTPLIVMSRSDNTGSGLTLASSPTAKKKYHIT
                     NVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQIFRKYVADEDLLIYSIDESILKV
                     TKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIATVGIGDNPLLAKLALDNEAKSS
                     KEFIAEWSYETVPEKIWKINEMTDFWGIGSRMKKRLNHMGIMSIEDLANWNPYTIKSQ
                     LGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQVLPKDYTRREEVELVVKEMAEQ
                     VAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKIPITDNTKELQNYCLFLFNKFYE
                     GQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEENLDRIIDKIRQKYGFTAIIHASS
                     MLEGARSITRSTLIGGHAGGLGGLHND"
     gene            1253..1600
                     /locus_tag="RD444_002589"
     CDS             1253..1600
                     /locus_tag="RD444_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549910.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
     gene            1915..2772
                     /locus_tag="RD444_002590"
     CDS             1915..2772
                     /locus_tag="RD444_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDT7549911.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            2792..3352
                     /locus_tag="RD444_002591"
     CDS             2792..3352
                     /locus_tag="RD444_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDT7549912.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            4484..5251
                     /locus_tag="RD444_002592"
     CDS             4484..5251
                     /locus_tag="RD444_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDT7549913.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            5277..5822
                     /locus_tag="RD444_002593"
     CDS             5277..5822
                     /locus_tag="RD444_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549914.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            5823..6389
                     /locus_tag="RD444_002594"
     CDS             5823..6389
                     /locus_tag="RD444_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549915.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            6404..6652
                     /locus_tag="RD444_002595"
     CDS             6404..6652
                     /locus_tag="RD444_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549916.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFLWLGQ"
     gene            6672..6911
                     /locus_tag="RD444_002596"
     CDS             6672..6911
                     /locus_tag="RD444_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549917.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            7282..7563
                     /locus_tag="RD444_002597"
     CDS             7282..7563
                     /locus_tag="RD444_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDT7549918.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            7553..7894
                     /locus_tag="RD444_002598"
     CDS             7553..7894
                     /locus_tag="RD444_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7549919.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         3135 a         1174 c         1676 g         2415 t
ORIGIN      
        1 agcgtggaat gtattgaacg aaatttagac ccaatgacta caccgttaat tgtcatgagt
       61 agatcggaca acactggttc aggtctgact ctagcttctt cacctactgc aaagaaaaaa
      121 tatcatatta ctaatgttag tagagctact gatttacctt atccattacc aaaagaactt
      181 catattgttc caccacgaat gaacttatat ataaaaaaaa atatggaagt taatcagatt
      241 tttagaaagt atgtcgcaga tgaagattta ttgatttatt cgattgatga atcaatttta
      301 aaagtgacaa aatccttaaa tttatttact agttcaggaa caagaagcga gagaagaaga
      361 gaattagcta aaataatcaa agatgagatt cagaaagaaa caggtctaat agcaacagtc
      421 ggaataggag ataacccatt attagctaaa ctcgcactgg acaatgaagc gaagagtagt
      481 aaagaattta tagctgagtg gtcttatgag actgtacctg aaaaaatatg gaaaataaac
      541 gagatgactg acttttgggg cattggatca cgcatgaaaa aaagattgaa tcatatggga
      601 attatgagta tagaagattt agcaaattgg aatccctaca caattaaaag tcagttaggt
      661 gtaatcggac ttcagttata tttccatacc aatggaattg atcgtacgga tattgctatt
      721 ccaccagaac caaccaaaga aaagtcttat gggaattcac aagttctacc aaaagattat
      781 acgaggagag aagaagtaga actagtagtc aaggagatgg cagagcaagt agccattcga
      841 attagacaac acaattgcaa aactggttgt gtacgcttag caattggtac gtcaattttg
      901 gaagaaaaat caggtttctc gcaccagatg aaaataccaa ttacagataa tacgaaagaa
      961 ttacaaaatt attgtctttt tttattcaat aagttttatg aggggcagga agtacgacat
     1021 ataggagtaa cttatagcag attagtatac acagattccc tacaattaga tttatttagt
     1081 gatccaagaa aaaagattgc tgaagaaaat ttggatagga ttattgataa aattagacaa
     1141 aaatatggat ttacagctat tatacatgct tcatctatgt tagaaggtgc aagaagtatt
     1201 acgagaagca ctttaatagg aggacacgca ggaggattag gaggtttgca taatgattga
     1261 atataacgat agaggacgtt taaaatggtc gggattttat ttgtctgatc atactgaaag
     1321 aatcaatgca gacaatgtac aacgtgctaa tcataatttg gcaaaagaac aaatgacgac
     1381 tgaagagatt gcgaaaatac tgagaactgc tgtcataaaa aatagacccg tctgcattca
     1441 aaaagaggaa cgaaacgctg aaggttacta tccagatgat gtagtaggaa aaattattgg
     1501 atatgacgag cttggtgtat ttgtggaaga agaaaaagtt cactatgatg aaattaggaa
     1561 tgtttctttc gcggaattat taaaatggag tcagatatga acattattaa aaaatacgaa
     1621 ttcggttata ttacgtatga acagctaata gaggaattat ggggatatgg tcaacaatta
     1681 attaatgaag tggggattga ttgcttttgt ttctatattg aatccggagc aggataccat
     1741 aagaacagat actatatatt tccatatcca ccaaaatagt ctcttagaga tttttaaatt
     1801 ttctccaaat actttcaatt attgtgaaat atttcctttt aattaatcaa attttttata
     1861 aaagtaatat ataaaataaa ttgcttgaag tattatattt agatatcata atttatggaa
     1921 gaaaaattaa ttaaaaagga tgagattata atggggttac tggttccgag agctaatact
     1981 tatactatac ctttaacgaa acgccaatta attgctaaaa gatatcaaag gattactaga
     2041 gccatcaata gagagttttg gaattctgaa agtgatactg ctcacagttt atacgtaggt
     2101 tcttatggga gaggaacggc aattagtact agtgatatag atataatagt tgaattaccg
     2161 atggcagaat ttgacagatt caaaaattat ttatcaaatg gcccgtctaa gttattacaa
     2221 gttataaaaa acgcatttca ggaaatacta ccaaatagtg atattagagc tgatggacaa
     2281 gttgttaaaa taaatttcca tgatggaatc aaatttgaaa tagttccagc ttttaatgaa
     2341 aaagattatt ggggtgagtc aaaagggttt atttatccag attcaaacat gggagggaac
     2401 tggaaagcta caaatcccaa aaaagagcaa gaagccatga aattaaagaa tacaaaaagt
     2461 aataatttat tatatgctac gtgtaaacat tttcgacatg tacgagatac agaatttacg
     2521 agttatcatc tttcaggaat agtaattgac agtttcgtat atgaggctat gggaaattgg
     2581 aaattcgttg aaaataacag tgggggtcaa aacattagtt ctgtatctta tgagacagct
     2641 ttattagaat actataactc acataaagtt atgggcggtt taaatttata ctctcctgga
     2701 agcaatcaat ttgttaattc tgatagtagt atcatatgtt tagaaaaagt attaaaaaaa
     2761 atagctttat aaagttttat ggaggaacaa catgggagac gaaaaaaagc gcttggaagt
     2821 tcaattacgt gaagcatatg gacgagtaac gtatacttac accactcatt taaagcaagc
     2881 cgataatatt atttctgaca ataaaatttt aaggtgtctt caaattggtt tgtcagccct
     2941 tactactgga ggggtactta gtaccattat tactgataat agtgagttaa cctttgttgc
     3001 tggattatgt tcaacaattt ctcttggatt aaacttatac tttaagaact ttgatcttgc
     3061 tgaagaagct aaaaatcatc aaattgttgc cgataaatta tggttgatca gagaagaata
     3121 cgtaagctta ctaacagatt taaatcgttt aagtgttgaa gatattgtga aaaaaagaga
     3181 tcaattaatg aacgaaactc atgaagcata taaaaatgcc cctaaaacaa atagtaaaag
     3241 ctatgaatca gctcagaaag cattaaaatc tgaagaagag caatttttta cagaagagga
     3301 attggataag atgttaccag agcatcttag aaaaaagagt tctaaatatt gaactctttt
     3361 tttatgctta aaattctctt gtaatttttc tattcaatca ataaataatc tatatttaag
     3421 aagaaatatg atgatagaaa catctcctga agccaccgca agccgttcta agcatagctt
     3481 agcccgacat tgctagatat gctcatctca ccatgaacgt ttcctaaagc caccgcaagc
     3541 cgtcctagcc attgcctagt ccgacatcgc tagattagtg catctcgtca taaaagtttt
     3601 ctaaagccac cgcaagccgt cctagccatt gcctagtccg acatcgctag attagtgcat
     3661 ctcgccataa aagttttcta aagccaccgc aagccgtcct agccattgcc tagtccgaca
     3721 tcgctagatt agtgcatctc gccataaaag ttttctaaag ccaccgcaag ccgttctaag
     3781 catagcttag cccgacattg ctagatatgc tcatctcacc atgaacgttt cctaaagcca
     3841 ccgcaagccg tcctagccat tgcctagtcc gacatcgcta gattagtgca tctcgccatg
     3901 aacgtttcct aaagccaccg caaaccgtcc tagccatcgc ctagtccgat tttgcttgat
     3961 cggttccatt tctgtttatt tagtgggtca acaccgtaat cttaaatagc catcgcctat
     4021 ttaagattac taggtctgct ttgaaggtaa aaagtctttc attcttcaag acttttaggc
     4081 tttcaagcta cgcctgaaag cattgaaatt catcgctctt tatttggttt tgccatttat
     4141 aattccttac gcccattatc tttttcgcct gtcttgcctt gtcagaagtg tttagagctt
     4201 atctcatctt tggattaata caagtggcgt aaattttttt ctaaacgcaa gcgttacgaa
     4261 aataatttac ttaaacactt gtattaatcc ggcagataag aacgcattct aatcactccc
     4321 gacaaggcaa gacaggcgaa aaagataaat aaatgaacga aaggaagaaa cataaaatgc
     4381 aaaaccaaat agcgatgaag aaaaaaccac ctccctctaa aacaatccaa acaaaaaaga
     4441 attttgaaaa accaaaaatc aaaaacagaa agaagcgttc aacatgagag atagaatttt
     4501 agaagttaat caagaaatgg taaacaagtt aacaaatatc agtgggaact atgaaaagta
     4561 cgatggtact ttacttgata actatatttt tcacgataca caaaatatca gaattggcag
     4621 aagtaaaccg agagcgttca ttctcatcaa agagaaatat ttgaatgaat ggaatagcac
     4681 ctatgaaatg gttatgactg ataactttga aaaggtattg gatttcagaa atgctttgtt
     4741 gggttctgat atgggaaata gcagtcttga atctattgaa gttgaaaaca agaccgagaa
     4801 tgaaggtgaa ggattgatta agaatgtctt gaaaatcgaa caaaaatata tcagtgaaga
     4861 tagaagtttt tatagagtca caagctcggt tggtctagca aactttgcta ctaacttaat
     4921 tggaaacaat gctagtgaat ctttgctagt gatgtgtctg aatacaaaga atgaggtcat
     4981 tgcttatagc gaagttttca aaggtggaat taatcaatca gtagcgatac caagagagat
     5041 ttttcaacta gctttgctaa acaattcagc aaaaatcgca gtatcacata atcatccgag
     5101 cggatatgaa tttccaagtg aaaatgacaa aagttttact acgaatttga aaaacgcagg
     5161 aaaaacaatt ggtattgaat tgttagacca tatcatcgta acaagtaaaa acacaagcta
     5221 ttactcattt caagaaaatg gttctttata aaaattagag aaaagaggaa aacaagatgg
     5281 gcgaaagaac acaagcgtta attacagtca aatatggtaa cagcaaaacg aaagcaagcg
     5341 tgtcgattca ttatcagtgg gggtatggtc gatatatgct gatggacgta ttgaatcaag
     5401 gaatttattt aatcaataaa gcatacgact tacgagataa atcaagagaa gaattagcaa
     5461 aaggaatctt tgatagaagt acaggagtta tcaattttgt tacctatgaa tctggagtga
     5521 gtgcttatga tgaaacaggg tttggtgggc gctatgaacc ttatcaagaa acgattgaag
     5581 atgtgatgaa gttttcagat aacaatgatg gctatgctca tttagagatg gtgttcgatg
     5641 aaaatggatt tagtctagat gataaaacca gattgcgatt gtttgatttt gagggtcaac
     5701 agataggact aaacaagttt tgtgaaagcg gaaaagagtt tgctacgaaa gattttatta
     5761 agggatacag attattgcta gaaaattatg gaataaacct agaagaagag agcgtgaaat
     5821 aagtgtttga aaaaagaaaa gaccagttcc ctaatttaga agcgaaaagt caaaaagtga
     5881 ttgatggtga aatgattgaa ttattccaaa aaagttatgg aaccatagaa actaccgcaa
     5941 gttgcaaata ctactttcaa ggaaagagaa acacgtttaa aggctttgcc gattctgatg
     6001 aagaattggc aaagaaaatc attgattggc ttgtcagcag tgaacacgta gcaagagaag
     6061 ataccgttag aacaacgatt aatggggaag tcctaacagg tactagatgt gtttgttgta
     6121 atcatgaatt tgtagaagat gaaggttgct accgaatcaa tgaatatggt gattttattt
     6181 gccagagttg ccacgagata gaatttccaa ccgaaagaag ttggtttgaa tacatgctac
     6241 aagattatga gatggaaaaa gaaaatggtg aagtagtttt ttgtaccaaa gaggaattga
     6301 gtaagagaag cgatgatgaa attgaagatt ggtgtgtgtg gttagtaggg gcagatgatt
     6361 gcgttgcata ttacacaaca gcagaatagg agtgtgaatc agcatggaag gaatggattt
     6421 tctagatcat gaagatttgg ttgattttgg ttatacatgg aaaggaatgg taggtattag
     6481 cagaagtctt gctaatgcct tttacgaaag aaattatgca gtatatgttc tttatgatga
     6541 cgatacagaa agccttgttg acgaggaata taaattggat ttagagaatg ttttatatgg
     6601 aatagaaaag gaagatttag caaaatatat ttttttatgg ttgggtcaat aagtgaaagg
     6661 aagatagaaa catgacaaaa tttaaaagta agggtatgca atggtgttct tattattatg
     6721 ttggaaatga tgaagattta ttgagaaaaa cccaacgaaa aggttatcca gataagatat
     6781 tgaaacaaat aggttattac atgccagaag agaggataga aaaagaattt tgtgaggttc
     6841 tttttgatag tgaacagcta ttagttttgt taacccaaaa taaattgatt tgccaatttg
     6901 aaaataatta agatgaggtg gtgaaccatg aatgaagata agatggtatt aagtgaatat
     6961 gctagattga gaactattaa ttctttagca tctcatttaa gttgttcttt cctaacaaag
     7021 gtgctaaaat taatttacaa atgtgaagat tttgtacaag agaagcacaa agacaagcct
     7081 actgcgaata ggcttgtctt tttttcattc attacgattc cgtatgtgtg ggttagcgac
     7141 aatttgttca attaccgtat gatatagcat cttaatttga ttagattact tgcaatgtag
     7201 tttattaaga gtataatata atcataaatg tggacaattt gagaaataat gagtgtttta
     7261 tactcaaaag gagtggatga aatgaaggtc aaaaatccaa ctgaaaaaag cagaatccaa
     7321 gtcggaatag acaagaattt aaaagagaac gcagaaatga tattggaaga gctaggttta
     7381 aatcctacaa cagcaataac aattctttac aaacaagtag tcgctagagg tgagtttcca
     7441 gtagagataa agctaagtga agaggagaag caaggaatca gattgcaaca gctaactaag
     7501 gacatgccag tagatgttct agatacagat gaaaagttag aagagtggtt cgatgaagcc
     7561 taatgagata gtaacggtct atgttgcatt tacagataaa aatggtggta aaagaagacc
     7621 tattttggtg gttagtgata aagaagatag agtagaattc tttggaatca caagtcaata
     7681 tgagaaaaaa tcagaccaaa taaagagggt gtattttcct attgcagaat gggaaaaagc
     7741 aggacttaaa aagcaatcat ggattgacgt agggtcttta aaggctattc ccaaaagccg
     7801 agagaacata tcattcaaaa aggttggtat gttatcagct agtgatacga aaaagctaaa
     7861 taggtttatc gagaaattac aaagaaaagt ctaacaaggc ttttcttttt tgtttatatg
     7921 tatattaaga tatacgttta tataaacgta tttatgtata tgaaaatata cgtaaatata
     7981 tacgtgatta taaacattaa gaaaacgtta agaattgtta aatagtgata tttagtaata
     8041 tacgaattat aagatatatt ttatatacaa tgtatgtaaa ataaataaaa tgtctgacgg
     8101 atattgaata gcaataaata acaataattc agacggatac agtgagtgaa gtagcttgct
     8161 acgaacgaac aggcagaatg aaacggcagt ttcattagag caagtcgctt gagacgtagc
     8221 gttaccgctg atgtaactgg caagttacat agagagagtt tgcttgcaaa cgaacgttac
     8281 ctacttgaat agaaataaaa aataacagat tagtgaattt tgaataggag ccacaactca
     8341 accaaccgct tgcggtgggt gagtcccacg gaggaaaaga cgaacgaacg gtcgcgtagc
//
LOCUS       DAPBAB010000093         8068 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPBAB010000093 DAPBAB010000000
VERSION     DAPBAB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.098"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..884
                     /locus_tag="RD444_002599"
     CDS             <1..884
                     /locus_tag="RD444_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDT7549920.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="RD444_002600"
     CDS             932..3061
                     /locus_tag="RD444_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDT7549921.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="RD444_002601"
     CDS             3036..3725
                     /locus_tag="RD444_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549922.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="RD444_002602"
     CDS             complement(4133..4819)
                     /locus_tag="RD444_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7549923.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="RD444_002603"
     CDS             complement(4955..5149)
                     /locus_tag="RD444_002603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549924.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="RD444_002604"
     CDS             complement(5199..5639)
                     /locus_tag="RD444_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDT7549925.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="RD444_002605"
     CDS             complement(5643..6488)
                     /locus_tag="RD444_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDT7549926.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="RD444_002606"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="RD444_002606"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDT7549927.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAPBAB010000094         7992 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPBAB010000094 DAPBAB010000000
VERSION     DAPBAB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.099"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..548)
                     /locus_tag="RD444_002607"
     CDS             complement(<1..548)
                     /locus_tag="RD444_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7549928.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAA"
     gene            complement(587..1705)
                     /locus_tag="RD444_002608"
     CDS             complement(587..1705)
                     /locus_tag="RD444_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDT7549929.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(1702..3801)
                     /locus_tag="RD444_002609"
     CDS             complement(1702..3801)
                     /locus_tag="RD444_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDT7549930.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETMNYFAQLFGQLSMSDDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSERYKDIACKLKESICKQMTIDGTF
                     GEQFLEGIGDEEKTTYSVKNYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDPLYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNH
                     QAFVTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIYIQRRKR"
     gene            complement(3852..4673)
                     /locus_tag="RD444_002610"
     CDS             complement(3852..4673)
                     /locus_tag="RD444_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7549931.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(4670..5479)
                     /locus_tag="RD444_002611"
     CDS             complement(4670..5479)
                     /locus_tag="RD444_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549932.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(5500..5994)
                     /locus_tag="RD444_002612"
     CDS             complement(5500..5994)
                     /locus_tag="RD444_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549933.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6006..6494)
                     /locus_tag="RD444_002613"
     CDS             complement(6006..6494)
                     /locus_tag="RD444_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7549934.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(6571..>7992)
                     /locus_tag="RD444_002614"
     CDS             complement(6571..>7992)
                     /locus_tag="RD444_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7549935.1"
                     /translation="VQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGP
                     VAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLL
                     IVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIV
                     ESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKM
                     TMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDE
                     SIEVFTKEYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAAN
                     LEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYH
                     FTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMK
                     YNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         2415 a         1849 c         1339 g         2389 t
ORIGIN      
        1 gctgccatcc cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt
       61 ggatgtcgct tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc
      121 ccattgaaca aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct
      181 aaccctttgc cagaaccagc aacatattca ccatagcaag ccgcattgac atctgttgtc
      241 cattcaatcg gaatattgaa atattgcttt agcgttccta caaaattgaa attttgccaa
      301 gcaagttttg gtgttgatgt gatatgaccg aaactcgttg atttgcattg aatatcaatt
      361 ggaccaaagc agccgacccc gatgcctgcc aattgttcct taaaaggttt aaaaaaatca
      421 atgacctgct tcattgtttc aactggagtt gttgtgggaa aactgactct ttccaaaatc
      481 gttaattcct cagtaccgac tgcacagaca aatttagtcc ctccagcttc aattgcccca
      541 tatatcatca taggttcctc ccttcgaata tgtgaaaaac acttcattaa cgcggcaagc
      601 gttctcgatc agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccagtaag
      661 cgacactagc aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt
      721 gtaaagtgac cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa
      781 gaccatgtcg tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt
      841 caaaacgatc tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg
      901 aatagttttt cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc
      961 caaaataatc ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt
     1021 agaatttaaa ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag
     1081 tacccacgta atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt
     1141 tggtaatgcg ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt
     1201 ccaccaaagc gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct
     1261 cgatcgtaat tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccccg
     1321 taggattaac cactataggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa
     1381 agaaatcccc taaaggactt tcaacagccg ggctatccga cccatcccaa taaattctta
     1441 agattaaatc acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca
     1501 tatgacgaat tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca
     1561 gcggaattgc gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt
     1621 tacctttctc gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt
     1681 gggtaatatt ttttagtata ctcatctttt tctcctttga atgtaaatcg tttttccctt
     1741 ttctacgtga agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga
     1801 aatactcaac tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt
     1861 tttccaagaa aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt
     1921 caatccgaaa tattgactga tcattatact gacaaaaagg cccgatgccc agccgcattt
     1981 gccgcagcca aagtctcgca caacttctcc ttgtttagcc tctaaacgat acggccacca
     2041 ccaccatgaa ccgtctaaat ctgccaaaca aatcagctcc tctaatcggg ttacgaaggc
     2101 ttgatggttg tgcatgtcac tcattaaaac agtgataaag ccaggaaaag tggcgccgga
     2161 ttctaatccc caagtgatgc ctttgatttc ctcgctataa gttggattga aagaactagc
     2221 agaaaaggtc atggtgttct gatataaagg atctttatta tctaaaaatt gataaaaagg
     2281 catcaacgta gtgtctgatt cttctccatc atgcatcaat aaattgattt tcccatccac
     2341 aatcacatca cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agttcttcac
     2401 tgaataggtt gttttctcct catcaccgat cccctccaaa aattgctccc caaaagtacc
     2461 atctatggtc atttgtttac aaattgattc ctttagttta caggcgatgt ctttgtatcg
     2521 ctcgctaaga ctcagttgtt ctagggcttc aaaaatttct gcaagtccac tacaggcctt
     2581 ccaaagacaa agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca
     2641 aacagatgga aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat
     2701 tcttgaagca ttcagtagaa cctgcggata ccgctcaaag aattttgtat ccccagtatg
     2761 ttcaaaataa agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat
     2821 acctcctgta aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt
     2881 tttttgacat aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt
     2941 attccaaatt cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg
     3001 tcgattcata ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac
     3061 tctatcgtct gacattgaca gctgcccaaa tagctgagca aaatagttca ttgtttcctt
     3121 cagccatttt tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc
     3181 cacaaaagca atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt
     3241 ttgagaaatc aacacatttt gctgatccga atacttctct tggtacaaag gcacatcttt
     3301 cactgaaact tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat
     3361 acttaaacgc ttattagcca gtattggagc aaaggatact acaatgacct taaacgtcga
     3421 ataatgatga atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc
     3481 cgattctgac aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt
     3541 ttgagaaagc tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg
     3601 ataaaaggtc aacgtttgaa tcgtcccgca aacatcaagg tttcctaatg tcttttgatt
     3661 acttacatca aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt
     3721 tgaagtagtc acatttccgt cgtttaactc agcattaaat aatgggtgtt tgggtgaata
     3781 aattacttca ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct
     3841 gcagcttcct actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca
     3901 aaaataattt tgatgacgcc gactttttta tctagtaatt tgaaacagcc caatgttacc
     3961 aataacggta caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct
     4021 tcgcccttca ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgcg
     4081 cctaccatga tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc
     4141 gcatacagct tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat
     4201 cgcatcaaat aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt
     4261 ccggcattgg ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac
     4321 aatgaatcgc caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt
     4381 tccacgtcaa aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg
     4441 acaaaggacg cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca
     4501 gctcgatctt cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa
     4561 ccaccgccgc ccatcgtcac cggattcacc gaagcataca tggtcatcga gcgccaaaac
     4621 atcttccgaa ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga
     4681 aatcctcctc ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa
     4741 cagatatatt ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa
     4801 ctgcggcaat tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa
     4861 accacacagc aatattttta ctccaaagca tccgtaacag catagccatc ccaactgccg
     4921 gcagcagatt tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga
     4981 cgcttttgac aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg
     5041 catagtggac aaaccacata acgaagtgca gattgatgat tcctttttga tcaccagatt
     5101 ttgcaaattt gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca
     5161 ttaactgtaa tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc
     5221 ctcccatttt gattgcgaac gctgtagcca acccagcagc caaagaaggc tccgccgtac
     5281 tagagattcc gatattgacg acacccataa aaatggcttc tagtgacgcc ccgacaatca
     5341 accccgtctg caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac
     5401 tcagcatgga catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga
     5461 ctacaaatgc atcatacata atttttctcc ttcctttttc taaaaatcac tcaaaataga
     5521 gatagctgaa cgtttttcca tcgccggtgt aggctgaaaa acggtatctg gataaagttc
     5581 aactaattct tttaagtctt gaatttctga cgtcgttaac atcacttcct ttgataaggt
     5641 tttcttatct tccgatggcg gtgtcatttt cccagcattc cccacgttca ttaattcgat
     5701 ttccgttggg aaagctttcg caacttttac cgcatctttg acattaggga ctaaaaccat
     5761 caagcgcatg tccttcgccc gcggatcttt taacaaatga atcgcgtcat caactgtttt
     5821 aataatgact ttgacattcg atggtgcagc catcttcaat gtcatctttt gaatatcgtt
     5881 ggtagccgcc tcatcgcttg ctactactaa cccggttaaa tttagactct ttgtccaagt
     5941 catcgctact tgtccatgaa ttaatcgttc atcaattcgc actgctttaa tcatttttct
     6001 tcctcctaaa aaaaatcttc ctcttcattt actacatgta cctcatcaag aatggtcatc
     6061 tttaattcct gttggcattt atttaccagt tcctgaatat cctcctttgt taattccttg
     6121 gttgccaata ctatttcgag cagaattggc agattaaagc cagacaaaat agttacagca
     6181 atctctttct ccaaaaaggt tttggtaatc ttctgattga cacttccgcc aaataaatcg
     6241 gtaaaaacaa aaatctgatc ctgctcgttc acctggttta caaatacatt caactcctgt
     6301 tcaaaatcac actcatccac ataagcatta atcaccgtaa tctcttgaac cttccccgtc
     6361 agtaattgaa tactgctaag aaagcctgaa gctaagtgac cgtgagtcgc tattagaatt
     6421 ttcttcatat ggttaattcc tttctttctg ttcactttta tataagcaat tcctgtgcca
     6481 acttttaaaa acattggttt aatcacgtta attcacatta aaaaaagtgt ctaacctaaa
     6541 aaaaacagat tagacacttt tttagacact ttatttttga atatacggtc ctaataattc
     6601 atagagcagc gaaagctcat aagcattgac tttaaaatta tatttcatct ccattgattg
     6661 aaaaatactt ctagaaacat aataaaattc atcttcatct ggtttcaaat cataattcgg
     6721 ttcttcagat ttttctgtcc gagccaagaa taaacgttcc atcatcaatg ccgtatgcat
     6781 atagagattt agttttacac ggccatttaa agtgaaatga tagtaatttt catatttaga
     6841 aatgactgtt tccacttcac tcatcacgat cgttgggttc agaaagttca aacggtccgc
     6901 aattccttcc aacgaaaaga attttactag ctcctgaatc aagcggttaa attctgccgg
     6961 cttcatttca ggactcaaaa tatgctccaa attggctgct ccatcaccat ctaaaatatc
     7021 atacacattg atattaggaa ttgaaaagga acgtggaaga tccgaagtcg tgatgaccaa
     7081 ttttgtcttg tcaaaataag tttcatcatt ttggtcgatt aattcacgta gttctctgta
     7141 ttccttcgtg aaaacttcga tggactcatc cgataaatga tgacgaaaaa catccttcag
     7201 tttatccgag atacccaatc ccgacatgca ggaaatgatg atgttgctac tttgagaaaa
     7261 gccttcaaag tattgaacat tgattgaata cttgtctgct gcatcctccg caatctgttt
     7321 aaatggcgca ttcatcgtca tcttcaagcc aatatcaagt gcaatggaag tcgtcaaatt
     7381 attcagaaga agtaactccc cttttaaatc gttcttgatc tgtgtgtaaa tttgatttaa
     7441 agagcccata tctacgatca gcactagact atctgtattc acttgccgtt tcacgaaatt
     7501 ccttgtcgat tcgacaatct ctgacacatt actctctact ggcatatcca acgcttcaaa
     7561 aacataattg ccgcataatt gattcactac tgcttgaatg ctgctagccg tactatctcc
     7621 gtgagcaagc aacaggccct tcagctcgat ggattcatct acataatcag ataaagtcac
     7681 gacaataagc agtcttagaa agttctcgtc cttttctgag agcggtggta aatgctccaa
     7741 gaactgatta cataagtaag tcgtccgagg tagatttgca gctaatgtac gatttatccc
     7801 ggttaagtct gtcttatagc ttggggaaat gacataggat ctgctaataa tttctgcaac
     7861 aggtccactc gtcgtcaatc cgtacgtttt tcctaaaacg gatacccaga cctttctaaa
     7921 catctgaatc atcaattttc tactcattga taaaaaagaa tccttatcaa tgtgatgcac
     7981 caaatgctgt ac
//
LOCUS       DAPBAB010000095         7760 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPBAB010000095 DAPBAB010000000
VERSION     DAPBAB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.102"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..187
                     /locus_tag="RD444_002615"
     CDS             <1..187
                     /locus_tag="RD444_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7549936.1"
                     /translation="LQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSF
                     ENMRIRIFLINQLINVR"
     gene            complement(366..695)
                     /locus_tag="RD444_002616"
     CDS             complement(366..695)
                     /locus_tag="RD444_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7549937.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(685..957)
                     /locus_tag="RD444_002617"
     CDS             complement(685..957)
                     /locus_tag="RD444_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDT7549938.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1408..2211)
                     /locus_tag="RD444_002618"
     CDS             complement(1408..2211)
                     /locus_tag="RD444_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7549939.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(2189..3313)
                     /locus_tag="RD444_002619"
     CDS             complement(2189..3313)
                     /locus_tag="RD444_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549940.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(3329..4144)
                     /locus_tag="RD444_002620"
     CDS             complement(3329..4144)
                     /locus_tag="RD444_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7549941.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(4134..5006)
                     /locus_tag="RD444_002621"
     CDS             complement(4134..5006)
                     /locus_tag="RD444_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7549942.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(5091..6380)
                     /locus_tag="RD444_002622"
     CDS             complement(5091..6380)
                     /locus_tag="RD444_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7549943.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6524..7035)
                     /locus_tag="RD444_002623"
                     /pseudo
     CDS             complement(6524..7035)
                     /locus_tag="RD444_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7102..7245)
                     /locus_tag="RD444_002624"
     CDS             complement(7102..7245)
                     /locus_tag="RD444_002624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDT7549944.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2406 a         1662 c         1193 g         2499 t
ORIGIN      
        1 gcttcaaaac cttctgacct atgaagaagg tatcaccaac gcaatgatct atccttattc
       61 caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag tatcctacgg
      121 atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat taatcaatgt
      181 aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt gattttgcac
      241 aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag gttcattctg
      301 acccagattt gattttgtac aacccatcag tccttattga caaagagccc ttctttttag
      361 tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg acttaattcc
      421 ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag agttcctacg
      481 tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc aacgtatttc
      541 agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg
      601 tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc agctgtgaaa
      661 gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt cttcaatttt
      721 ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa tattatggat
      781 tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct caacaatttg
      841 cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa taatgctttc
      901 tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt tctccacctc
      961 tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac aactactttt
     1021 gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag tgctaaatgg
     1081 ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg aacactcaac
     1141 gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa
     1201 ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg aagaccgata
     1261 tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg tttattgata
     1321 gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta aaaagtgaat
     1381 gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt ttttcatgcg
     1441 ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt ttccgtcaat
     1501 taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc
     1561 tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt cagcactgct
     1621 accttgatgg atatggccta caaaaatacc taaaatatca ctttcggcaa tcaattcagc
     1681 aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg gatgatgaac
     1741 caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt gaatcatctg
     1801 tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg aaataattcg
     1861 taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt caacactcat
     1921 tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt aaggtgtggt
     1981 tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc ccccttcatg
     2041 tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt cttgtaacaa
     2101 tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag taaacaagtt
     2161 agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca tggctattcc
     2221 ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa
     2281 aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc caagtaggtt
     2341 tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca taactttgac
     2401 ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta
     2461 cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt tgtttttgcc
     2521 aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga tcataagtaa
     2581 ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt
     2641 tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc aataaaacaa
     2701 tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca aaagtttgtt
     2761 tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa cgcacatcca
     2821 aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc gcacgagcca
     2881 atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct tggtatggcg
     2941 ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct gtcttaattt
     3001 tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga tataaagcat
     3061 aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt gctagttgtg
     3121 tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag
     3181 tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg gccacagcta
     3241 aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt cttaattcaa
     3301 gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca aaagtcccga
     3361 taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta attaaactaa
     3421 tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc tgcatcgcca
     3481 acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca tactcattcc
     3541 aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta agaggaaaaa
     3601 caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca tctagaaggc
     3661 tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt
     3721 gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc aacaaataat
     3781 taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata aagtaccaga
     3841 ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa gctacgccta
     3901 acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt ttgataattg
     3961 gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt atcaaactag
     4021 taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata aacaataatg
     4081 gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag cgtttaattt
     4141 tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac agcaaatatt
     4201 aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc aaaagccttt
     4261 tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg agtgatgatc
     4321 ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa taaatatagt
     4381 cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat gggtacacga
     4441 tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc aaaaatgaca
     4501 atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc aattacccaa
     4561 ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt
     4621 ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac caaagaaatc
     4681 acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat aactattcct
     4741 aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag aacggtatag
     4801 gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt tacatagttg
     4861 gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa actcaaatag
     4921 atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc tggcgccaga
     4981 tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat gtcaaaagca
     5041 gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt
     5101 aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt acctgataaa
     5161 attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc tccaggaaat
     5221 gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat ctgattttca
     5281 gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg tggtaaataa
     5341 ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt taagaaatcc
     5401 caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc gccaccagca
     5461 ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc agtaaaagta
     5521 ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc gacgttacca
     5581 tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg gtcttttacc
     5641 atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt agctttgcca
     5701 tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca attatcagca
     5761 ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt agaaaaagtt
     5821 tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt taatatatct
     5881 tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg tttaccatct
     5941 ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc agcattatcc
     6001 ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc tgcaaaataa
     6061 tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc aacagaagct
     6121 tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac aaccttcact
     6181 ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc acctagggta
     6241 tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt tgttgtcttg
     6301 gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac aacccctata
     6361 acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg ccatttttct
     6421 aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg taaataattc
     6481 gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct aagaataaag
     6541 cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt tgattataaa
     6601 atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt gaggttatat
     6661 tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact gtccatacag
     6721 aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac catccttcat
     6781 gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg atcaaatctg
     6841 cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta taaaatatat
     6901 gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca atttgtggcc
     6961 agattgatag gtttgtgcca gcgaaaaaac ggccatttcc aatccctctt cttttaaatc
     7021 acagttaatg gtcatggttg ccttctcatt ggccactgct tgaaacacct gctcaagggt
     7081 taccgaatct gggacaactt cttacctgtc atgggctagt attagcgtac catctgtttg
     7141 ctgacgcaca tctacttcaa aacaaagctg gcgatccaaa caaaagcgaa gaaatgccca
     7201 gctattctcc tccgtattgt cactaccaga gtgtgcagtg atcatggtca ttttgacccc
     7261 tcctttaatt ccgtgatttt ctcccacacg tccactatac cgatgaagcg ttaatttctt
     7321 gttaatttct tgtaaattcc ctgcgttttt tcagtaaact agttgtaaat cttgcatgct
     7381 ccttcctacg aactcatgaa tcgtcgattt tgcccttcta gctagtttca ccaattaagt
     7441 agttctacat tccccgcttt taacctacta cacctttttt tgttttcagc tattccttct
     7501 tcttgctgaa agtccttgag gttctttctt acaggatacc atcaaggtgt tcaatggctt
     7561 attcccattg aatgaccgag acttactatc agatttctgc tcgttgaacc aagtctttga
     7621 tacaggattt cttccgtcat cttctcacaa ataccacctt gcgtttctct agcgattggt
     7681 cgatgtgcac cctcccagtg gactgtcact accccttttt gctatgccta gcccacaaaa
     7741 aaagctgtcc tcacagacag
//
LOCUS       DAPBAB010000096         7741 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPBAB010000096 DAPBAB010000000
VERSION     DAPBAB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.103"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(32..1180)
                     /gene="nagA"
                     /locus_tag="RD444_002625"
     CDS             complement(32..1180)
                     /gene="nagA"
                     /locus_tag="RD444_002625"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDT7549945.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1256..2092)
                     /locus_tag="RD444_002626"
     CDS             complement(1256..2092)
                     /locus_tag="RD444_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7549946.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2276..3493)
                     /locus_tag="RD444_002627"
     CDS             complement(2276..3493)
                     /locus_tag="RD444_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7549947.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     ITSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3642..3821)
                     /locus_tag="RD444_002628"
     CDS             complement(3642..3821)
                     /locus_tag="RD444_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549948.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3876..3959
                     /locus_tag="RD444_002629"
     tRNA            3876..3959
                     /locus_tag="RD444_002629"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:gag)
     gene            complement(4199..5569)
                     /locus_tag="RD444_002630"
     CDS             complement(4199..5569)
                     /locus_tag="RD444_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549949.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSLQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6284..6574)
                     /locus_tag="RD444_002631"
     CDS             complement(6284..6574)
                     /locus_tag="RD444_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549950.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6782..6985)
                     /locus_tag="RD444_002632"
     CDS             complement(6782..6985)
                     /locus_tag="RD444_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549951.1"
                     /translation="MNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLE
                     KAKDEVEDTADKSQQIVEEHLRK"
     gene            complement(7082..7474)
                     /locus_tag="RD444_002633"
     CDS             complement(7082..7474)
                     /locus_tag="RD444_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDT7549952.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEIN"
BASE COUNT         2366 a         1529 c         1182 g         2664 t
ORIGIN      
        1 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
       61 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
      121 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
      181 tcatgactgc ttgttctggt gttgctaatc cccaatctac aacattttta attgcttctt
      241 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
      301 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
      361 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
      421 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
      481 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
      541 caaatacact ggcgccagct tcaacagctt tttgcgcttc ttctagcgta gcgttactgt
      601 gacctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
      661 gacgttccgg tgctaaagca atttttttga ttaaaccgcc tgaagcttct tgccattcat
      721 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
      781 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
      841 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
      901 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
      961 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     1021 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     1081 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     1141 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     1201 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     1261 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     1321 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     1381 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     1441 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     1501 ttgatcgttc gtcagctgat tggtgcaaca gttgaatgct tcgactcaaa taatagcgtg
     1561 cttcagccgt ttttcctgca acaccatata tttttccaat gcaactgatc agttgtattt
     1621 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     1681 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     1741 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     1801 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     1861 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     1921 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     1981 gacttaatac actttgtgaa caaatatctt gcgaaagcat tttttgcgtc attttttgtt
     2041 cttttcgaat ctctttaatg atcgtgccaa aggactcgat taacgtttcc ataagtatcc
     2101 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     2161 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     2221 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
     2281 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
     2341 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
     2401 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
     2461 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
     2521 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
     2581 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
     2641 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
     2701 tatagccttt aacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
     2761 ctacacgatg agatttggat agaccattca gcgtaacaac gaacaaatca ggcgctaatg
     2821 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
     2881 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
     2941 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
     3001 tttttgatgt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
     3061 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
     3121 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
     3181 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
     3241 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
     3301 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
     3361 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
     3421 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
     3481 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
     3541 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
     3601 aaaacgtaag tattaaaaaa acacaaacaa gcgttaagaa cttaattgtc atgatagtca
     3661 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
     3721 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
     3781 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
     3841 gtaaaacctc gctatcatta ttgtgttatt attacgcaga tgtgttggaa ttggcagaca
     3901 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
     3961 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
     4021 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
     4081 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
     4141 taatcgaaaa acgagtaaaa tccctcacct aatactagag cgagggattt tttgtatatt
     4201 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
     4261 taaattcatt tttttgtaaa ctcatgcttt tcattagctc tttaactgcc attagtacta
     4321 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
     4381 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
     4441 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
     4501 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
     4561 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
     4621 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
     4681 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
     4741 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
     4801 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
     4861 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
     4921 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
     4981 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
     5041 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
     5101 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
     5161 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
     5221 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca tacttgcaaa
     5281 gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc ataaagtgtt
     5341 tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc ttataatgtt
     5401 ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact acgtcatccg
     5461 ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc cctattagta
     5521 acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt tttatactcc
     5581 ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa
     5641 agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat
     5701 agtcaacaaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
     5761 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
     5821 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
     5881 gtcgaaccag gacgttctcg tgtgaaagac gaggcttctt caatttactt ttacgactat
     5941 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
     6001 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
     6061 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
     6121 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
     6181 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgaa tccatctttc
     6241 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
     6301 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
     6361 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
     6421 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
     6481 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
     6541 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
     6601 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     6661 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     6721 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     6781 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     6841 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     6901 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     6961 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     7021 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     7081 tttagttgat ctcatctagt ttgacattga tttcattttg cttgctttgt agttgtgcat
     7141 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     7201 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     7261 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     7321 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     7381 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     7441 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     7501 ttacatacta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt
     7561 aattttaata taaatactat caaatactca tttaatcctc cttttttatc tatttaaaaa
     7621 atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt tatggtgaac
     7681 aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga ttactgtatt
     7741 t
//
LOCUS       DAPBAB010000097         7628 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPBAB010000097 DAPBAB010000000
VERSION     DAPBAB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.104"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(16..708)
                     /locus_tag="RD444_002634"
     CDS             complement(16..708)
                     /locus_tag="RD444_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7549953.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1145..1714)
                     /locus_tag="RD444_002635"
     CDS             complement(1145..1714)
                     /locus_tag="RD444_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7549954.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="RD444_002636"
     CDS             complement(1788..2111)
                     /locus_tag="RD444_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549955.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="RD444_002637"
     CDS             complement(2265..3077)
                     /locus_tag="RD444_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549956.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="RD444_002638"
     CDS             3431..4279
                     /locus_tag="RD444_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDT7549957.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="RD444_002639"
     CDS             4424..5365
                     /locus_tag="RD444_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDT7549958.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="RD444_002640"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="RD444_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2273 a         1512 c         1372 g         2471 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctt
//
LOCUS       DAPBAB010000098         7600 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPBAB010000098 DAPBAB010000000
VERSION     DAPBAB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.105"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(943..1356)
                     /locus_tag="RD444_002641"
     CDS             complement(943..1356)
                     /locus_tag="RD444_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDT7549959.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="RD444_002642"
     CDS             complement(1812..2297)
                     /locus_tag="RD444_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549960.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="RD444_002643"
     CDS             complement(2436..2651)
                     /locus_tag="RD444_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDT7549961.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="RD444_002644"
     CDS             2875..3675
                     /locus_tag="RD444_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7549962.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="RD444_002645"
     CDS             3694..5562
                     /locus_tag="RD444_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDT7549963.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="RD444_002646"
     CDS             5555..6046
                     /locus_tag="RD444_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDT7549964.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="RD444_002647"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="RD444_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDT7549965.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="RD444_002648"
     CDS             6606..>7600
                     /locus_tag="RD444_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDT7549966.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1212 c         1443 g         2238 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaatatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAPBAB010000099         7535 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPBAB010000099 DAPBAB010000000
VERSION     DAPBAB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.106"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            13..207
                     /locus_tag="RD444_002649"
     CDS             13..207
                     /locus_tag="RD444_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549967.1"
                     /translation="MIFAAVAAVASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVIL
                     AAASIVVSMFSMFGQTPQGL"
     gene            278..2332
                     /locus_tag="RD444_002650"
     CDS             278..2332
                     /locus_tag="RD444_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549968.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            2332..4944
                     /locus_tag="RD444_002651"
     CDS             2332..4944
                     /locus_tag="RD444_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDT7549969.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNEEISEMI
                     GKRLLLESEKVVKE"
     gene            4941..7472
                     /locus_tag="RD444_002652"
     CDS             4941..7472
                     /locus_tag="RD444_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549970.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
BASE COUNT         2839 a         1160 c         1514 g         2022 t
ORIGIN      
        1 aaaaataatc tgatgatttt tgctgctgtt gcggcagtgg cttccattgc tcttggaggt
       61 attttcttct tctttgggcg aagaggagct gaaaacggga agacacacgt aggttatgta
      121 ttgttggggg taggagttat tttagctgcc gcttctattg tagtttcaat gttctcaatg
      181 tttggacaaa ccccacaagg actataaaaa aacaagtaga aggagtaaaa agtacgtggc
      241 tactttttac tccttttttt atggaaggag aatattcatg ttttttacga tttcaggcaa
      301 tgcaaaattg gataaagagt taataagaaa agcaacgaag aaagtgggaa agcgtacttc
      361 ctttttaccg aaaacaatta aatattctgt cattgaaacg gaagaacagc tacatgtgtt
      421 aggacaagtg ttagaacaag agaatcaatc ttctgttaaa ttgactctca cgttacatga
      481 taaagaagga aaaatagaaa aggctggcac agaatacgtt tttggctcac tggattatca
      541 aatttcagaa ggtttttatc ctacattgat tgatttaatc cgcctagata tatttaaaga
      601 tacggatgaa accatgagtg tggaagagtc ttatcgaaac gaaaaaattt tacaagtttt
      661 agaagaagag ttagaaagcg accaaagaaa agtgaatcaa gtgagtgaag tcgaagcaaa
      721 cgaagaagta ccatgggaac gcccaatctt tccaaaggat gaatcaaaag aaaaagagaa
      781 aggaattatt gacgaacaag ctgacatttt gactcataaa tcaatatttg aggaagagaa
      841 cgaggtaatg ggtcaattgg aggtagatcc ctttgaagaa acaatggagg atgaagagac
      901 ggccgaagta gaagttgatc cagttgaagc taatgaagac ccaacatgga ctgaagggga
      961 ttctcaggaa tatattgaac cacttatcag tgaagaagta gagaaaaatg aaacagagat
     1021 acaaaaggca acggtatttg attatatttt ggatcaatat ccgccagagc atgaatggat
     1081 gaaagaaaaa aataaattat ttttaaatga actttattcg aaatatcaat tacctcaaac
     1141 ctatcaagca tttttagaga gtaaagaaga attaatctca cagggtaaag gcacactttt
     1201 agaacagtta gagtctgtcc aatcaacaaa ttggcatgag cttgcacaag aagatttatt
     1261 agctactttt gaagaacgtc aacaagaaac agatcaagaa attaatgagt atgcgacgga
     1321 acagtcgtcg aattgggaga aagcaaaaga ggaactaaat aaagaagaac agcgagtgat
     1381 tgaagaagaa gtacaaaaaa ttaaagagcg gtacgaaaag aaacgtgaca atgcgtattc
     1441 cttgtgtgta gaaaagatca acaattttac tcagacgaat caagaagcat tatcgaaaga
     1501 aaaagaaaac ttattaaaag aacgtataaa agaacgaaaa gaagaatttt accgagggtt
     1561 gcgtactgat aagctaagga ttagcaaaga attaaatgaa caactgaata aactctatca
     1621 aactacgact gaagttctga tggaaaaaca cgagcagatc caacaagaat tacaagcaca
     1681 aattccaaaa tggaaaaaag agtatgaaat tcaccaacaa gaattgaaag aacaagaaga
     1741 aaaacaacgg aaaaaagaaa aagagcgcga acaattagaa ctaaaaaaac gtgaacagga
     1801 tcttgcggaa caaaaaatgg cgatagaaaa acaacggcta gaacaagaaa aggaaaaaga
     1861 agcagctgct cgtaaggaac gtcaagaaca aatggataca ttgcgacaag cacagttaaa
     1921 ttatctgaca catcctcaac cagtagctgc tgttcaaacg ccagtgaaca accaagcaaa
     1981 tttaactcct tcaccccagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt
     2041 aagcttgctt ctaggtggag gaacagtatt tgcttttaat catatcaatc aatcttcaca
     2101 ggctgattct gtagccacaa cggcgtcaat caaggaagaa gcccaggaac aagcaagaaa
     2161 agagtatgaa caacaattag aagaattatt gaatcgtacc aatcagtcaa caacagagtc
     2221 aagcatggaa aatagcaaag agagtgattc tagtaaggct aaagaaggat caagcgattc
     2281 aactgtacaa tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt
     2341 agagaaacaa cgaagcgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct
     2401 catttctatc gctatactcc ctcaggagaa gagctaccag aaaaacatga caaacgcatt
     2461 tcgaaaaaga ttttttatag tggacttgta tacctccctt tagagtattt agtaacaaga
     2521 gtaccagaag ttttcaaccg attggaaatt gttaagggaa gcttacaagg aataaatctg
     2581 tttcaattac aggaaatgcc acaggtcttt cattttgatt cagctggttt ttggaatcca
     2641 tttactccta caactagttt gggtacacgt ttagcaattt cgacagtttg tgtggttcca
     2701 gctgtactta tgggattgaa aatggacatg tggtttcgtc ctctagcaga tggacaaaaa
     2761 ggggataatc gattaatgac cttaaaagaa atccaacaga tttatccgca aatacctgaa
     2821 acaaaaacta cttttccagg ttatggaggt attccaatca cacattacaa aaaatattgg
     2881 tacattcaaa cagatactgt taatacgtgt attgttggta cctctcgatc agggaaaggt
     2941 cagattgaag tgttagccac cattgacaat ttatctcgtg cagaaaaaca aagttcaatg
     3001 gtcgttaatg atccaaaaac agaattatat gttggtagta aagatgaact agaagctaga
     3061 gggtatgatg tatatgcctt taatatcacc gatccactgc aatcaatgag tgataatcca
     3121 ttggcactca tcgtgaaata ctggaaacga ggagacatag atactgcaac acagttaacc
     3181 aatactttca caaatacgat ttattatgat gccaacgctt cagataacaa atactttaac
     3241 gaaaacgcgc agaaagctgt caatgcgctt atttttgcgt tgttagaaca agctgatcaa
     3301 acaggagatt actccaaatt gacaccgaat aatctcgttg agcttctttc tgagattggc
     3361 ggttttaatt attatccaga tccagacaat gaattcaaac agttgaatgc cttagatgaa
     3421 tttatgaatc agttgccacc tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt
     3481 ggatctgata aagcgaaggg aaatatttta tcaacggcaa tcacgggatt gaatccattt
     3541 actctaacga aaattgcaaa gatgacttct caatcaacga ttgattataa atcggttggg
     3601 tttccaaaat acttagattt gaaattacat gaaagtcttt taaatcaacg agtcgtagta
     3661 gaattttatc ataagggaaa gaaatttcat gaagaacgtg tcaaagtagg ctataaagga
     3721 tttagtgaaa tcaattttga ttgtcattta gttgcaggtg atcaagtttg tttaaaatac
     3781 gatcatcaag gaaaagaata tgtggcttgg tatgaatttt ctcaaagagt attgaaagac
     3841 gaagagggta atatcgttta tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa
     3901 aaagaagccg ttctaacatt agatgaggaa aaaagtaact tattagtaga gtctattcgg
     3961 atgcactata ccgataaacc aatggctatt tttatgctta cacctgatta tgatccatct
     4021 aaccatatca ttgttagtat ttttcttagt cagttatata aagaattatc tacgcaatgt
     4081 gttaagacta aaggagataa atgtcaccgc aggattcatt ttattttaga tgaatttggg
     4141 aatatgccac caatggataa tatggaagga atcatgacgg tcacagctgg ccgaaacatg
     4201 ttgtgggatc tgtttattca atcttatcag caactttatg cgaagtatgg agaacaaaac
     4261 gcagcgatta tcaaagaaaa ttgtcagaca catatttata ttatgtcgac agatgataat
     4321 accatcgaag aattttctaa aaaagtgggg aataagacgg ttgaacaaga gaacagtacc
     4381 atcaattcat taggtatgaa tactcatatc aatcgcaatg ttgattcaga tcgtatctta
     4441 actcctgaaa gaatttctac ttttttagaa ggtgaaacga ttgtactcgc tcctttaaaa
     4501 cgacaagatt tgaaaggtca taaaatacgg ccgtatccta tttttaatca tggggaaacg
     4561 aatcttcctt atgcttatca atatattgaa gatttcaatc catcgtggga tatcaatgat
     4621 atagacatac attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattatgat
     4681 tatttcctac cagatgaata tgctagaaat ctctatcatg gactgcatga tcaattagaa
     4741 gaaaaaaacg aagaaaatat gttttataaa atcgttcaat ctcttgagaa tgagacggct
     4801 aaaaaattgt taattcaatt ttacgaagaa caagcagcag atcgacttca agaatatgtt
     4861 gaaaagttga tcaaaaatga agaaatttct gaaatgattg ggaaacggtt gttattggaa
     4921 tctgaaaagg tggtgaaaga atgatcattg gaggaatttt ttctggcata gccgactggt
     4981 ggtcaggctt attcggagaa ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag
     5041 attatggtaa ttatctgcag tatcaagatg tattcagttg gatttgtaat acttttttat
     5101 ggggtttgat taaagggtta tataatctca atactttact agaaaaaaca atttatcaaa
     5161 gctttgattt aaaagactta ttgaacgccg ctggtgttaa tgaactgttt caaagtttga
     5221 ttagtaaggt attggctctt ttttgtgtcg tcacattaat ttatttaggc ttaaaatttt
     5281 ttatgagtaa acatccacca aaaatcaaaa atatcttggt taatctattt atggccatga
     5341 ttttgatttt tggtggtagt tctttaatcg atgaagggct aaatattagt aaaaactttt
     5401 acggagacgt gactgcagca aataaagcag ataaaacggg ttcacctgct tttcaaatta
     5461 ttcaaaataa tgtgattgat gtgagtacgt tactagaaaa tgatccgaac aaagtagaac
     5521 acataccaac agacaaaaga aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca
     5581 atgggattat tactcctaaa caagctaaag aaatggcgga taaagataag agtggtttat
     5641 cagatttaca aaaggatcgg ttaagagcgt tacaataccg actggaactg gatgataatg
     5701 gaaaagaaat tcctgtgcaa atcagtgatg aaggcttagc caaatatgtt tatacttcag
     5761 ggtatcgtcg atacattagt agtccaggga ttattttagc tggtgaatct tctttggcgg
     5821 tggcctattt atttatccta tttacgatcg ttacttgcat tattgagctg gtatttaaaa
     5881 aattctattt agtgattgcc gcgtcaacgg actttgagac aggacaacgg atgaaaagtg
     5941 ctatcgaaga tatttctcaa tccttcttgt tattggcatt tacaatttta gaattacgaa
     6001 tctatacctt aatgctttca ggtattggtg atttgcatgc ggcaggaaag atcaatggat
     6061 tcttgtatgt tgtcgctttg attgtactaa ccattgcgct attcaaaggc tctcaatcag
     6121 ttacgaaaat tttcggcgtt gatacgagtc tgaaaaacgg cagtaattct cttcttagca
     6181 tgtttgcctt aggaaatatt gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg
     6241 ctggtaagag tggtttaagc catttaaatg atattcgtaa aaatggattt ggccatcgaa
     6301 aagatgttac ccctgataac ggggaagaac aatcagacga taaagctaca actgtaccta
     6361 atagtaagaa aacagtttcc gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg
     6421 cagaaggtct tggttacgtc aatgaacgtg ggttaaaagg aacagctgaa gatcttacag
     6481 aaaaagccaa agacacctta gaagataaat tagacggcac gagagctaaa gaattggctg
     6541 aagctgtgaa gaatccatcc gaagcactag aaaaacaaaa agaaaaagct tcagaagtca
     6601 aagaaggact aaaagatgcg tatggtgaag gacaagtcaa tgcagcagtg aaagctaata
     6661 caacaaccga aaaagaaaat caagaaggta tagtgattcc ttcagtcaaa gatcaccaag
     6721 aagcgttgca ctctaatgaa aattcgattg aagagaaaaa ttccatagaa gaaagaaatt
     6781 cagaagaagg aaaaaatgga cagttaccaa aaactgatcc agaacaatat cgctataaac
     6841 ctaacagtga ccttgaaaat gagaagcgaa tttcagattt atcaaaagta aatcaagaag
     6901 acaaagcaga aaatgaggga cagttaccaa aaactgatcc agaacaatat cggtatagat
     6961 ctgttaactc cgaaaatgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac
     7021 aaacggatga ggattccatc gttctgccaa atgataatgg aatcgaagtt ggaactgaaa
     7081 aatcaacgaa aattggttta cctaaggcac aaaatttacc aatgaaagat agtcgtttga
     7141 atcaattcaa ggataatgat caaagttttg ttcatcaatc acctgcaggt actgttcaaa
     7201 tgaaaccaag tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttatctg
     7261 aaagtggtga gacagggaat gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa
     7321 aacctattca aaacgttcat acaggttaca agccagcaaa tgtggcattt actgcaccaa
     7381 caaaaataaa tgtgacaaga gaaactaaaa acaggaacaa tgcattgaaa gaacaactat
     7441 tgagggatat caaacaggat atcaaaccat aaaaggccta caagttaggc cttttcttta
     7501 tatgaaaagg agaaatcaat tatggataaa tatat
//
LOCUS       DAPBAB010000100         7370 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPBAB010000100 DAPBAB010000000
VERSION     DAPBAB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.107"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(200..1084)
                     /locus_tag="RD444_002653"
     CDS             complement(200..1084)
                     /locus_tag="RD444_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDT7549971.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEADGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1179..1616)
                     /locus_tag="RD444_002654"
     CDS             complement(1179..1616)
                     /locus_tag="RD444_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549972.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(1833..2744)
                     /locus_tag="RD444_002655"
     CDS             complement(1833..2744)
                     /locus_tag="RD444_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDT7549973.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(3121..3759)
                     /locus_tag="RD444_002656"
     CDS             complement(3121..3759)
                     /locus_tag="RD444_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7549974.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(3763..5745)
                     /locus_tag="RD444_002657"
     CDS             complement(3763..5745)
                     /locus_tag="RD444_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDT7549975.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFILLFVERFNQAFHTDYATNIFE
                     DFENSYSYSVISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHETPKEIAGED
                     YQVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            5925..6587
                     /locus_tag="RD444_002658"
     CDS             5925..6587
                     /locus_tag="RD444_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549976.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALRIAHNLV
                     KF"
     gene            complement(7068..>7370)
                     /locus_tag="RD444_002659"
     CDS             complement(7068..>7370)
                     /locus_tag="RD444_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7549977.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMLSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGIIGGLALARKAIRNK"
BASE COUNT         2353 a         1391 c         1115 g         2511 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gtttaaaata
       61 tgtattttta tggcaaattt ttctgttagg tgaaaatttc ttttttgtac aaaaaaaggc
      121 tgtgacaaaa gtcttgtcac agtcccttat tccgaacaac cgatgtcctc ctgacaactc
      181 ttcggtcaca acctccattt taacaataaa cctgtaaatg tgagggtaac actttgattg
      241 tcacaggtaa tttatcccct tcatctccat caacattcgt cgtcaattct tcctcatcag
      301 ctaatgacac aactaattgc tcacaagtaa gataggctag atttttcgta gattcatcta
      361 ctccttttaa taggtctggc aatgttttaa gcgtatcgat caaagaagaa tcttttaaat
      421 aaaccaaatg gagcttacca tcatccactt ttgcatttgg aagtaatgtt tcaaaaccgc
      481 ctactgagtt agttagtcct atcagcaaga tactgctctc aatttcttcc ttctttccat
      541 cagcttctac gtgaaacgag taggattgtg tggaagctaa ctgtttgatt cctgacatga
      601 aataagctag ttttccaaat ttcgtcttct tctccgcatc tacatcattg attgcttcag
      661 ggatagagcc aattgcgacg acattcataa aatagtcgtc atttattttt ccaatatcca
      721 atggcttgac tgattcaatc gaaaaatgat tgatcgcttc ctcaggttct agcggtatcc
      781 ctaatgctcg agccaagtca ttcaccgtac ccaatgggaa aaatccgaaa tttggccgat
      841 gttcttgttc agcgattcca ctgattcctt cgttgactgt tccatctccg cccatgacaa
      901 acacactatg atacccttcc gtagccgctt cgcgagtaaa gttttttgca tcccctgctt
      961 tttttgtatg caagacgacc acttcatcaa acaccgattc caattttgct atcgctaatt
     1021 gctcaaattc ctttgcttgt tcaccgcctg aactggggtt taccactaat aaaacttttt
     1081 tcatcacttt ctttcccttc tgattgattt ctttaatata acgatagcat aaagaaaaaa
     1141 attaggctgt gatgaagtac tgttcatgaa acattcattt attttttgac aagataaaat
     1201 acccatacta tgaaactgag gaaaattaaa acaatgatat aggaaataaa agaaaatgat
     1261 aggcattgta tacctaataa agtaaaaatc ttaggtggca gataaaaaca caaaaatagc
     1321 aagagaatgt aatagcgttt cttccacttt ttgttatcag accttatagc gatcagagga
     1381 ataccaaatg cataactgaa aaactcttga tgtactgtta atatgtcacg accttgaata
     1441 ataaatgcaa caatccccgc tataattagt gtgcctaata ccaaaacgga aaattgatga
     1501 atatatttta tatacaaatg atccattgta cttcctgcct ttttatttgt gagaaaaaca
     1561 aatccgtata cgtgaataac ttctagaaat aaaagaaatt tgcaaaacca ctccatttat
     1621 ctacttctac gtgcattttt taatatcgtt ttctttattt aagattatac agaaaaatat
     1681 ctagggtggc tggattctaa gtaaaaaaaa tttttaaaat aaaaagaact aaatcaagta
     1741 ataattaaac tactgttgat ttagttctaa taaaaagatt tagatgaaca gttttattga
     1801 tttaaatagt tcatattttt tatttatacc gcttatttac tacttgctgt actagaatct
     1861 gaagtgaatg tactcaaaac attttcaaag tctttgtctt tgatttttac attagcgtct
     1921 ttcaatactt cgccaattac tttattctgg aaggtactgt cacttaattt tgtgtctgtt
     1981 gcaatttctt tcaattcttt tttgtattta tccatgtcgt tgcctttgtt ttggtttttc
     2041 accattttaa ccacataata ttccgtagta taagttgaag cattggtaga agtgatcaca
     2101 tcagaaactt gaccatcttt caacttaaag gctgcttctt tgacttctgc cggaaccgtt
     2161 gtagtagtgg aatcaaattt cacttttccg ccatcttctt ttgtcgtagt atcagttgac
     2221 ttgtctttgg ctaatttaga gaaatcgtct cccttatcag cagctttctt cacatctttt
     2281 gcatcatctt cacttgctac ttggataatt tgagcttcca cttctggatg gaaagattcc
     2341 caagccgttt ttaaatcttc atcggtcaat ttgatatgtt tctttagacc ttcttgaaag
     2401 gctagacttt gtttgatact gtctttgaat gtttgttctg tgtaacctgc agatttcaat
     2461 tgtgaatcaa atgaatcacc caattgtttc tttgtttgat catattggga atcgatttct
     2521 ttggttgata cgtcatctcc atatttatct tcaaatactt gataaacgat caactgttgc
     2581 acagcggaat ctgctccttg catttttttc aagctatcca ttacttcttg ttcgctgatt
     2641 gtaccgcctt tataactaac ggctgttttt tcaccggcat tcgtacagcc tgacaataag
     2701 ccagtgccta gtacgatggc aagtgctaaa aatctttttt tcatttatca attctcctac
     2761 ttttcgtttt ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc atcaagaaca
     2821 cctttactaa tctgctcgat acgtcctgaa tagatataag gctacacaat ccagaaagtt
     2881 cgctcagcag aataaatgtc aagtagcaca tcacacctta atatactact tcttttgtta
     2941 ccgtttttca agtctatctt gcgttccgta tttaacgtaa ctaattctta gaacaaatgt
     3001 tatcgatttt gtttttcttc ttccgctgtt gggatattca aatcagacac ttttatgttc
     3061 gattttccag acattttttt gttgttttta ttagaaaaag tttgtttttt catttgtttt
     3121 ctaaagcatg atcttcttgt ttcctatttt ggtcagatat ggatcatgcg tgaccacgat
     3181 aactaacgta ccttgggaag cctgttgcga taagatttca acgacttgct ccttatttcg
     3241 tttatctaat gatccagttg gttcatcggc taaaatgatt ttgctattct tcaataagac
     3301 tctcgcacag gcgattcttt gcttctcacc accagataat gtgtagatag gcgtacttag
     3361 cttcttgttt atatttactt ttttcaatgc ctcatacttt gctgttcgct tatctgaaac
     3421 atttaaattc gaaaacttca atccaatatc taaattaaaa tcgattgttt catattcaat
     3481 caacccaaaa tcttgaaaaa gaaaagagag ggcatttctt tttatcaagc gcttctctct
     3541 tccagttaaa tgttttgttt cttgattaaa taaataaaat tgccctgaac tgaattgatc
     3601 attcaatcct aatatattca ataaagtact tttccctgct ccgctctctc caactacagt
     3661 cacaaaatcc ccagcatccg cagtgaaacg atccaaatga agaatcgttt tgtttcgaat
     3721 gatttttttc gcacctttta tcactatcaa cggctccaat tgttaacctc cattcatcag
     3781 caataactga cttttctttt ctgtagacaa aataaaaaca actgaaagaa taaaatcaat
     3841 gaagctaaag aaaaaaatat taaaaagata tttaggctct ctttctataa gaaaaaggaa
     3901 gattacactt gtcgttcccc atttgatgag gttcatcatg aagaaatctt tatacgtttc
     3961 aaagagcgaa taaccacttg aacgaaagat agcgattttc cgacaattgt agacaaaata
     4021 tatgcataac atgacatagc tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg
     4081 tatcaagtaa tgaaatgatc tgcttaacac tttgacagct atctcttcag tctgagtata
     4141 cggaaatgat aactgcaagt tatcacgtaa ctgactttct tcaatcagct caaataacct
     4201 ttctttcgac aaagagccta tcttgatctt taacggtgga tagatttctc cgctgagaat
     4261 atttctttcc caatacgtac tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc
     4321 gatccagggg atagaaggaa taaatgtata aatgggttga ttgtttttta taaatagaaa
     4381 tttcggttct ttttcactga tttcttgata atattctgtt atttgcgcca ggtcattttt
     4441 taaatctgta tgtctatatc gttcagggat caatataaca gggtgcgttt ctttttcggt
     4501 tatttggatc aaccttccag agacatcaat caaaggaaat ttcttaagat aattcagatt
     4561 tatttttatc cctctgccaa actgttcatt ttcttcattc aagtaatctg atacattgac
     4621 taatatactt ccgtttttat tcaaagtttc atataattcc tgatcggctg ctgatgcaaa
     4681 acgattggaa taaataaaat ccacgtggtt tttcccgatg gttactggat aaaatacctg
     4741 atagtcctca ccagcgattt cttttggtgt ttcatgggaa acagctaact gtgaagaaga
     4801 aaaaacgata gttgctaatt cgacattggc aacggtcaaa agaagagaaa acaacaatcc
     4861 aaatgtaaaa agaatgtatt ggatatagtt gaacgcttct cttaacgaag cgattttttc
     4921 aactaataaa agagaaatca aagagatacc atatattccg ctgaacaaga aaacgtacat
     4981 ctggaacaaa taaagaaatt gctccttata atacataaaa ataaaaccac caatgattcc
     5041 tgcaattatg aatgtaataa tgattttctt tttccccaat aaacaccaca atacttgatg
     5101 taatgaaaag ccattattca acaataaact gatttcttta ttcatcaata agaaaaagga
     5161 tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat aattatatac
     5221 gtctgtactt gaaataaccg aatacgaata gctattttca aagtcttcaa aaatattagt
     5281 ggcataatca gtgtgaaatg cttgattgaa tcgttccaca aatagtaaaa tgaaccgttc
     5341 ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt ttattttgat
     5401 cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct tctcgtgaga
     5461 agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta tcttgaagtt
     5521 ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag aaagctcagt
     5581 ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt gcccaataaa
     5641 atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt catttgtccg
     5701 atagagtacc tgtttattta tactaaccaa ggcaaaacca ctcatgatac cggtcactat
     5761 gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt acttaaatga
     5821 ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaaa tgtataggtc
     5881 atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac atcggagaaa
     5941 aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc gaaaaaatcg
     6001 gtgtttccga taaaacaatc tctagttggg aaaattctcg tacaatgcca gaccttgaaa
     6061 tgctgtcact tttacatcag actttaggtc tgccgattga ttttccttct attgaatcag
     6121 ctaccgaatc aatagaacca acattgccgg atgaacctct tgttatcaag aaatcttatt
     6181 catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt
     6241 tggtctatct ttttcttatc aattatcaat ataaaaatga aacggttgat cgattcaatc
     6301 cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg gaaaattcca
     6361 tcgaacattg ggtatgtaat gatatctttg ttcatggtac atggctgtct tttcacgctg
     6421 gagaggtaga aaaaggtaag aatcatgctg taatcaaaca caaaggaaaa cacgtcatga
     6481 acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc ctcatccaag
     6541 actatgacga tgccttaaga atagcacata atttagttaa attttagttc ttttttatat
     6601 ttctacagcc tgttaataag taaaatttta cttttgtata atgatttacc ccacagatcc
     6661 ttccatatag attttcaaat tacacactag tttaagataa aacaaacaag gaaaaactta
     6721 ccatttactc aaaaaattaa ctcatataag tacattagaa tgtaaaatag actggtcaaa
     6781 atctggtcaa aaaagtagat gtgacacaag ttcgcattca aagcgatgat ccatgatttc
     6841 gtaatggtca attatatgga tcaaagccac gcgacaagag cgcaatggat gtctctaccg
     6901 attggttgaa taaacgaaat aaatagaatg tgaaaaagga tcggacaaat gttccagttt
     6961 gctcgatcct tttttatttg aattcatttt cgctttatca aacatcattt gattactcag
     7021 ttaaatactt ttgacatgag taccaatgaa acatttattc attaaaatta cttatttctt
     7081 atcgcctttc tagccaatgc caatccgccg attattccag cactatcacc taattcgggc
     7141 gtaacaatgt agctagcaat tggtggtatt tcaacatatc catttataag ctcaacaaat
     7201 ttatcctgaa cttttttctt catgtgttct tgtttcatta caccaccgcc aaaaataatt
     7261 ctatctggcg aaagcatcag tgttgtattg tatgcacatt gggcaaggta aaaggcttca
     7321 atctgccaaa acgaatcgtc agccaataaa ttctggcctt ttacacccaa
//
LOCUS       DAPBAB010000101         7367 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPBAB010000101 DAPBAB010000000
VERSION     DAPBAB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.108"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="RD444_002660"
     CDS             complement(99..1229)
                     /locus_tag="RD444_002660"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDT7549978.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="RD444_002661"
     CDS             complement(1878..3080)
                     /locus_tag="RD444_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7549979.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="RD444_002662"
     CDS             complement(3140..3343)
                     /locus_tag="RD444_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDT7549980.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="RD444_002663"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="RD444_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="RD444_002664"
     CDS             4880..5692
                     /locus_tag="RD444_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549981.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="RD444_002665"
     CDS             complement(6716..6913)
                     /locus_tag="RD444_002665"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDT7549982.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..>7367)
                     /locus_tag="RD444_002666"
     CDS             complement(7059..>7367)
                     /locus_tag="RD444_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7549983.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMFSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGIIGGLALARKAVRNKQP
                     "
BASE COUNT         2268 a         1477 c         1133 g         2489 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaa
//
LOCUS       DAPBAB010000102         7185 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPBAB010000102 DAPBAB010000000
VERSION     DAPBAB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.109"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            445..855
                     /locus_tag="RD444_002667"
     CDS             445..855
                     /locus_tag="RD444_002667"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7549984.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            848..1303
                     /locus_tag="RD444_002668"
     CDS             848..1303
                     /locus_tag="RD444_002668"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7549985.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            1320..2051
                     /locus_tag="RD444_002669"
     CDS             1320..2051
                     /locus_tag="RD444_002669"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7549986.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            2068..2880
                     /locus_tag="RD444_002670"
     CDS             2068..2880
                     /locus_tag="RD444_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7549987.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(3096..4379)
                     /locus_tag="RD444_002671"
                     /pseudo
     CDS             complement(3096..4379)
                     /locus_tag="RD444_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4454..5865)
                     /locus_tag="RD444_002672"
     CDS             complement(join(4454..5310,5310..5865))
                     /locus_tag="RD444_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7549988.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            complement(5846..6037)
                     /locus_tag="RD444_002673"
     CDS             complement(5846..6037)
                     /locus_tag="RD444_002673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549989.1"
                     /translation="MGKYSFEFKSTIVQEYLEGEGGLGYLAKIQGVRSKKGTRMDQSK
                     VRPKNLTFGGRYLYGKISF"
     gene            6554..6782
                     /locus_tag="RD444_002674"
                     /pseudo
     CDS             6554..6782
                     /locus_tag="RD444_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            6893..>7185
                     /locus_tag="RD444_002675"
     CDS             6893..>7185
                     /locus_tag="RD444_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7549990.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYARSFTTRIGTLELHVPRTRDGKFSPTIFERYQR"
BASE COUNT         2182 a         1276 c         1252 g         2475 t
ORIGIN      
        1 tatagataaa ttattttctt taaacttata ctcattttta gatattttat aagaatactt
       61 catgtcttcg ccagtaatat aaagaacatc tgtctctcca tttggacttg ggttatctat
      121 tttgggtgta acatactata catacaaccg atacataaaa gatttgacat ctggacaaaa
      181 cacaatgctt tacttcattt ttataggctt tgtttacacc atcacaaaaa tcacctccta
      241 atttacaaat tcattaaaca aacagaaata aaaaatcacc acgatagccg cgttcaaaaa
      301 aatttacaaa agagaatttt agttaaagtt tttagattcg ccccactaat taccaaaggt
      361 attttattta ttcttaatac gaaataattt attgacattt agattaccaa tggtatatta
      421 tttataaata aatggaggtg gattatgtac acaattattc ttgcaagcca caaaaattta
      481 gcgaaaggaa tgaaagagac agtcgaatat atccttggcc ctgcaccatt cataaaagta
      541 atgactgcat acacagacga taactatgat attgaaaaag atacgaatat tcttctcgat
      601 ggaactgata aagtccttgt catcactgat ttacttggtg gtagcgtaaa taatcattgg
      661 atgaattatg tttacgaaaa aaaattaaca aaaaaaatta cagtaattgc aggtatgact
      721 ttgtccttaa ttatggaatt aagtatgaac attgaagatt ataaattgag agaaaaaatt
      781 tccatgatta ttgctgaaag ccaaaagagc attataaact gttcagaatt aatggaggta
      841 gaagataatg attaaactat taagaattga cgatcgcctg atccacggtc aagttgcaat
      901 ggcttggact aagtccgtat ctgctgacta cattattgta gttgatgacg cctctgctaa
      961 tgacaaaatg aaacaaatga ttttatcatt agcaaaacct gtatcaacgg gactagatat
     1021 tattacttca gcagattttt taagtacctt tgaaaaaagg aaaaatcaaa atttgatgat
     1081 agtcactagt tcccctaaaa gcgcatttga aattgtaaac gctatcccag gggaagtcga
     1141 ttcaattaat atcggtggtt tacggtacgc cgagggaaaa gagaaagtta acgagtatgt
     1201 ggctatcaca tcagaagaca aaaactactt agcaaaatta aaggagtccg gcttcaaatt
     1261 aattatgcag gctactccaa ctggaaaagc gaagatttat tagaaggtgg ggaataagaa
     1321 tgattgaatg cattttaatt ggcatagttg ggtatttaac gacaatagat gaaagatatt
     1381 ttggggcatc tatgatgaat cgaccgataa tagttggacc tgttgttgga cttatcttag
     1441 gggatctcca tcaaggcatc ttgataggag cagcattaga agcaatgttt attggcattg
     1501 taacgattgg tgctgcacta ccaccagatg taggtgtagc tggtactatt gcaacggctc
     1561 tagcaataaa aagtggagca ggtgctgatg tagctgttac attggcgatg cctttcgctg
     1621 tattggcaca aggattaaac atgttggctt ttacgattaa ctcctttacc gtttcacaag
     1681 gtagaaaagc aattgaaaac cgcaaattat catcttttca gaagtggcat ttttttccat
     1741 taatcgttcg cttaccttcc gcagtactta catttttagt agtatatttt ggtgccaatg
     1801 ctacagaaag ttttgtaaat attatgccag aaaaattaat gaccggtttc accgtagctt
     1861 ctggtttact ccccgctgtt ggatttgcca tgctaattca aggaatgatt gacaaaaaat
     1921 cagttcctta ttttttcatt ggataccttt tagcggcata tttaggagtc cccgtagttg
     1981 gtgtagctgt tgctgcaatt atggttgctt tattagttat ccaaactaaa cgcaatcctg
     2041 taaatattta aagggagagt gataattatg ggagattcta aattaactaa aaaagaaata
     2101 aacaaggttt tctggcgttc atttgcttta cagggatccg agtcatacac tgatatgcaa
     2161 ggaatgggat atagtttttc tatattacct gctttaaaca aaatatatca agggaatgat
     2221 gaaaaatatt atgaagctgt ttaccgaaat acggaatttt ttaatacaac gcctcatatt
     2281 tcaaccttta ttatgggtat gtcattagca cttgaagaag aaaatgcaaa aacagatcaa
     2341 tttgaaactg aaacaatttc atcgataaaa gctgcattga tggggcctgt agctggtatt
     2401 ggtgatagtt tttactgggg aacatttcgc gttattgctg ctggcatcgg cttatcttta
     2461 gctgcccaag gaaacatttt aggtccaata ttgtattttt caatttatac gctgttacat
     2521 tttcttggca gatatggctg cttacgtatt ggatatcgga tgggagcaaa cgccattgaa
     2581 aaagcatttg agaacaatta ccttgaaagt ttctcgttag cagcatctat cgtaggttta
     2641 atgtcaattg gagccatgac ctcatctctg gtacgcttta ctattcccgc cgtatttaaa
     2701 tcaaaaggtg gagaaataaa tgttcagcaa atgctcgatg atatttttcc aggaattctc
     2761 tcattggggc taacattctt tgtttacttt ctattaaaga aaaaagtaaa ggttctttgg
     2821 attatacttg ctatcttcgt ctttagtatt gttggagttt atattggatt attaggttaa
     2881 ttaatccaga ataaaactag atcagacatc aacttaaacc ataaaatacc caaagataaa
     2941 tgtaaatatg agtaaaaaga gcatggtata aattattagg atctataatt tatgggtctg
     3001 atgaatcaga attgattctg tagtgaccta aaaaagatag atgttttcgg agtggagaaa
     3061 attcactccg tttttctttg cttttcttga tgattttaga cagctttgaa tgctattact
     3121 ctaaattgca ccagactctg ccagtttagt ttccttttca tacgctcatg gttgtagtaa
     3181 tgaatataac catcaatggc ttgcttcaat tcttcaaaga tacgatagtc tcttccataa
     3241 taaatttcct gctttaatag actaaggaag ttctcaatga tcgaattatc taagcaggtc
     3301 tccttacggg acatactttg gaagatctta tgttctttca acttatgact ataagctttc
     3361 atttggtagg tccatccttt ttggagtgaa aagttcgacg atagatacat tccgttgcgc
     3421 agatagcttc ctctaaagct tccatgatag ccaacgcact tggcttctca gaaatgcgat
     3481 aaaataaaat ttcactgttg tacatatcta aaaatggatc aagatacagc ttcttttcac
     3541 gattggcacc gtatttatcc atttcaaaat attttagttc tgtcgtatct gttgtaacct
     3601 tttgatgaca gttgcttgtg taaaagcgtc gatgaattcg attttttgta atcatgccga
     3661 ttcttccacg ataagtatta tagcggcgat attttctcgt aaatgatcgt acttcaatac
     3721 tcagctttcg aatcaaacgt tgtacttttt tcttgttaac atggaaccca cggtcccaca
     3781 attcctttgt catacgtaaa tagccataat ctttatattg acttcttatt ttaagcattt
     3841 cctcttcgat ttcttgatct gaatttcttc gatcaaatcg tctttgccaa tacataaagg
     3901 ttaattcagg aaatttcaag gtttcaagaa gctcaactaa tttgaatgat cctcggaggt
     3961 tgtcgatgat tcgtgcgatt gattcgttcg tctttgttag acttcctgtt ttctaagctt
     4021 cctcaattct tttaaaaagg tattctcaat ctgaaggaaa cgcacttatt tttccaattc
     4081 tttgatacga ttatgctctt taggcgtcag tttgcttgtt tcttcctttt attctttctt
     4141 agacaaaatg gaggaggacc ctttcatttt tgagagcccg tgcaatcctt ttgaacaaaa
     4201 attttcatcc aattgacaac taacgcaggt tggtttatcc ctaaatgatt cgccacttca
     4261 cgataggata gctcacccgt tttttataac tctatcgcat ctatcttgaa ttgaacagaa
     4321 taacttcgct tatttcgctt acgcaagagg ccttcttctc caaattctcg ataagcattt
     4381 gtagcgaccc caaaaagtta gacttttatg gagtggagaa atccactcct tttttgtacg
     4441 ataaaattaa ctgttagaca gctgttttta cagcttctcg aaactgaacc ggacttcgcc
     4501 aatttaattt ctttttgatg cgtttatggt tgtaataata gatatattgg tcaatttttg
     4561 ttttcaattc atctaaactc cgatagactt ctccatgaaa aatctcttgc tttaataaac
     4621 caaagaagtt ttccataggt gagttatcta aacaattccc tttacgagac atactctgga
     4681 agattttatg ctgcttcaat ttattcttat aagcattcat ttgataggcc catccttgat
     4741 ctgaatggaa ggttctacga aatggacaat catttgtcat ctggattgct tcttctaatc
     4801 cttccattat ggctagtgcg tttggttttt cagaaatccg ataagataga atttcactat
     4861 tgtagaaatc catgaaagga tctaaataca gctttttctg acgaacgaca cctgtggtgt
     4921 ctacttcata atacttaaac tctgtggtat cagttgtaat tttttggtga cagatatttg
     4981 tataaaaccg acgatgaata cgatttttgg cgacagtgcc aaccttccca tgataagaat
     5041 tgtaccgtcg agattttcga gtgtaagact ttacttcgat acctagtttt tttatcagac
     5101 gttgaatctt ctttttattg acatgaatcc cttgattctc taattctttt tttagtcgca
     5161 gacagccata gtctttatgc tccttgcgaa tttcaagcat tttttcttca atttcttggt
     5221 ctgggtttgg tctgtcaaac cgtttctgcc aatacatata ggttgcttta ggaaatttca
     5281 atgtttcaag aagctcaact agtttgaatg atcctcggag gctggcgatg attcgtgcga
     5341 ttgattcttg cgtctttgct gggcttcctg ttttcgcagc ttcctcaact cttttaaaaa
     5401 ggcattctca atttgaaggg atcggacttg cttttctaat gccttgatac gctcgcgttc
     5461 ttcagttgtt gtattttctt ttttgttttc tttttttcta gtcaaagtaa gtggtcgccc
     5521 tttctctttt gagagcccct caatacctgc ttctctgaaa ttacgcatcc aattggcaat
     5581 aagcgcaggg ttgttcattt ctaaagtgct ggcaacttct cgataggaca tctcacttgt
     5641 ttgatataac tctagcgcat ttaacttaaa ttgaacagaa tatttctgat tttgatgctt
     5701 tttcaataga ccttcctctc cgtattcacg ataggcattc acccaatcac ttacttgaga
     5761 cttgcttgaa ataccgtatt tgttggcaag atgccgatat tcgcccacgc cactaagata
     5821 ttctttaacg atttttagtt ttaattcaaa aggatatttt gccatacaaa taacgacccc
     5881 caaaagttag attttttggt ctaacttttg attgatccat ccgtgtacct ttttttgatc
     5941 tcactccttg tatttttgct aaatatccta atccaccttc tccttcaaga tactcttgaa
     6001 cgattgttga cttgaattca aagctatatt ttcccataaa aataccatcc cccctgaagt
     6061 tagattttgg tctaactttt tagggtttgg acatcaccag tcccattttc attcacgtaa
     6121 tgcaagaaac tgactgaagt cattaaacaa aaatctgagc tagaatgaag ttcattctga
     6181 ctcagatttg attttgcaca actcatcagt cttcgtaaaa tatattttgc ctaataagtt
     6241 tcttttaaag tatctataaa acacaaattt ttttgaataa catattcctt ccggaattta
     6301 tttcgttatt gtgtgatttc tcagagtaca atggatgtat ggaaaggatt caacgagaat
     6361 ttgctaatca gtcctgtagc tttgtctacc ttcataagtc tgttatccaa agcgctgtgt
     6421 ttactatcaa ctcattagct cttttattac cgtattctat taggcgaggt cgtgaccaca
     6481 cagtgagttt ctgtggaatc ccacagtatc gaagcatttt ctagaattta tatcgaaaat
     6541 aggtttttat tccatgaaat tatttgttgg taatgacgtt agttctgaaa aattagacgt
     6601 ttgcttccta acagacgacg atcagctatc tatattgact gaaaattctg ttgctaatga
     6661 cattgaaagg gctccattga cacgagaact gattcttgtg ttcaatgaaa acaatcactt
     6721 cggtcaaatc gttattgaga tggaattaaa cgtccatgta cagctttcat ccttcgatgt
     6781 ttttgattac gtccatataa ttgtgtaaaa gtgaagcatc aaaaaagcca ttgatctcag
     6841 ttaggagtac aatgtttttt gcagaaaaca tactcgaata ggactgatat caatgactca
     6901 agtacatttt acttttgaaa gcgaagaaat tcaagcaatt ataaacgaaa gcggtgcgaa
     6961 tgataccgca aaaacactta tgactatcat gtttaatcag ctgatggaag aacaacgaaa
     7021 tcaatatatt caggcaaatg cctatgaacg ttcagaagaa cggcaatcac aaagaaatgg
     7081 ttactacgct cgttcgttta caactcgaat tggtacgtta gaacttcatg ttccacgaac
     7141 cagagacggt aagtttagtc ctactatctt tgaaagatat cagcg
//
LOCUS       DAPBAB010000103         6756 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPBAB010000103 DAPBAB010000000
VERSION     DAPBAB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.111"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(47..1204)
                     /locus_tag="RD444_002676"
     CDS             complement(47..1204)
                     /locus_tag="RD444_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7549991.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1207..1563)
                     /locus_tag="RD444_002677"
     CDS             complement(1207..1563)
                     /locus_tag="RD444_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7549992.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1861..2835)
                     /gene="sgrA"
                     /locus_tag="RD444_002678"
     CDS             complement(1861..2835)
                     /gene="sgrA"
                     /locus_tag="RD444_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDT7549993.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(3031..3870)
                     /locus_tag="RD444_002679"
     CDS             complement(3031..3870)
                     /locus_tag="RD444_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7549994.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4055..4942
                     /locus_tag="RD444_002680"
     CDS             4055..4942
                     /locus_tag="RD444_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDT7549995.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5536..6231)
                     /locus_tag="RD444_002681"
     CDS             complement(5536..6231)
                     /locus_tag="RD444_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDT7549996.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6215..6613)
                     /locus_tag="RD444_002682"
     CDS             complement(6215..6613)
                     /locus_tag="RD444_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDT7549997.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2125 a         1324 c          993 g         2314 t
ORIGIN      
        1 aatctttttg caatgtctta attttggcat aaacaatctg cttttcttaa ttaacgaaat
       61 ccaatttttc caatatggat ctatttttct cattcctgat ttttatgatt tcagctttca
      121 gacgcgccca atcatttttt gtaggttgtc cattccagta aaatatctgt gtattcgttt
      181 gatcatatgc ctttttgtcg ataggataat cgctaatgac aatatcaggt aactgatcag
      241 cggatgcact atattttatt gggcgttcag tcatagataa tatccttttc tttgcccata
      301 attcctgtgt tttaccatac tttgattgaa tatagaatat gacaggttgt tcgtgtttta
      361 taaaaatatc cctgactaac atacagtatt gaaaaatcaa ccgtctgttc ttttcataat
      421 aagtattaag ctctggaatt ctgcgagcta ccccactaaa aaactgttgc atgattggat
      481 acatcactgg aaatatatgc tgaaaatctc tgtcttctgc tgattttcca aaagcatcca
      541 ccttcttttt tgtcccaaaa acccttgaat acaaatggat cattgccaaa ttgacatata
      601 gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa ttttctacaa
      661 tagacgattc aatccagccc caactttctt gtactttatc tggagtatcc aacaaatctt
      721 cttccaaata ggtgttcatt acaccaaaca taaaataaag gtaagcttgc tctgttggag
      781 tgatgtgatt catcgaccag aagtttgctt caaaaaaagg ctctagtatg taatgcgtaa
      841 actcttcttt tgttatcaat ggatgcacaa atttgttcat ctcttctggg acatacgtaa
      901 tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag taggctgatc catttctttc
      961 tatctatact acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt tgacgtttat
     1021 tttttatact tgataatata tcagaatgat tttcgtcgaa tgactgccaa tacatcaagt
     1081 gaaaaaagta tcggatacgg tattcttctc cttctatcgg attacctttt ttcaagctgc
     1141 aagataaatg atagtttttc aaaatagtat taagttctct taattttctt tgaatcgtac
     1201 gcaaacttat ataatttttt gactaagatt ttctaaggat tgataagtac ctttgtaaag
     1261 tgaatccagt aaaatataac caatcgttcc ctctaaaagt atagaataaa ctttactcag
     1321 taaaaactga gaagaaaact cgacttttat catcttgttt tctgaaagaa aagataattt
     1381 atattctgga tacctttcgc aaaacgattc tgcttgttga atcaccgaat tcatcttagc
     1441 cagggtgtag tttagatcta aagcgatatc cttcaccaca tataatttct cgtctctttc
     1501 ataacaatag ttcaaaaatt gatatacttg cctttcattg atatcaataa attcttcaaa
     1561 caaagtacat actccttttt atctattatt tagacttatt ccgacagcta catgaataac
     1621 gtacaaatag attcgttact gatttttcag agatactacc atgcgcagtg tgcattgtca
     1681 attattcttg ctcttatttt ccatttgtgt tcctgttatt tcctgtttct caaatatgtt
     1741 agtaaacaaa aatagatctg gcaaagctac aacttcgcca ggcaatacag cactctcgaa
     1801 tgaaatattc actcaaaaat ctatcgtttt attcaatttt agatctgttg cttttattct
     1861 ttatttcact cgatggaaga gagaacgaaa atagcttgct cctacaatca aaatgagtaa
     1921 cacacccatc catgtaatta gcttgttcaa ggttctacta ccagtttctg gtaaatcgct
     1981 ttttctatta tctgtttctt tcgctttttc ttgatgatca attccacgag aaacaaaatc
     2041 aggctgtttt agcacacttt ttaggttgct ccctgcattt ctcttttgtt ccttagttgg
     2101 tatgataggt accaaatcaa gttcattatt tacttttcct gtgctgcttt cgctgctcgt
     2161 gcttcctgtg ctactctcac tactcatgct tcctgtgcta ctttcactac tcgtactctc
     2221 cgtactgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact
     2281 gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc
     2341 accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc
     2401 aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa
     2461 aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat
     2521 cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat
     2581 aactttttct gaacttccat taccatccca accatatgtt agaacagcat gtacatcact
     2641 agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata
     2701 aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga
     2761 agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt
     2821 cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt
     2881 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
     2941 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
     3001 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
     3061 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
     3121 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
     3181 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
     3241 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
     3301 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
     3361 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
     3421 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
     3481 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
     3541 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
     3601 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
     3661 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
     3721 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
     3781 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac
     3841 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
     3901 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
     3961 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
     4021 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
     4081 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
     4141 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
     4201 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
     4261 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
     4321 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
     4381 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
     4441 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
     4501 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
     4561 agaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
     4621 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
     4681 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
     4741 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
     4801 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
     4861 catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta aagatcctta
     4921 tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc aaaatttgag
     4981 aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc acaacttcat
     5041 cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat catgaggata
     5101 taatttaaaa aagtttttta tttctatcac atgaatcaat gcgccattaa gtagattctt
     5161 tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc ataaactcaa
     5221 actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac ttgttcttct
     5281 tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt catttgtttt
     5341 tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt agaggttagg
     5401 gaatacatcg tttaaatgag aaataagtaa tagttagtaa tagtcaccca aactttaaat
     5461 taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta tcgtttattt
     5521 tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa tcgtttctgc
     5581 ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa tcgagggcat
     5641 ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc cagatcgaat
     5701 ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg ttggtctttg
     5761 cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa tgccttgatt
     5821 ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag ctaaataccc
     5881 ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt tcaacttttt
     5941 gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt ttcccagttc
     6001 ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta ttgtctgaat
     6061 atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga agaagtttga
     6121 cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg
     6181 ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca tatagctttc
     6241 ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa tgcggcattt
     6301 ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca tcaaatttta
     6361 tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact gctgtgatta
     6421 catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt cttacctgat
     6481 tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct ccaaataaaa
     6541 tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt acaaccgttt
     6601 caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag tttttattca
     6661 aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat ttgtttcgat
     6721 attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DAPBAB010000104         6755 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPBAB010000104 DAPBAB010000000
VERSION     DAPBAB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.112"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            690..1658
                     /locus_tag="RD444_002683"
     CDS             690..1658
                     /locus_tag="RD444_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7549998.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="RD444_002684"
     CDS             1669..2484
                     /locus_tag="RD444_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDT7549999.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="RD444_002685"
     CDS             2552..3262
                     /locus_tag="RD444_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7550000.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..4797
                     /locus_tag="RD444_002686"
     CDS             3298..4797
                     /locus_tag="RD444_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDT7550001.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNVSTEDFFNYCRLEESG
                     VTVTSGTDLPLFITDIPESIIRAVFRRFPEGEEVWEKETSFTTTELLKSFTSNSFKVN
                     GLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMAAEVIATYLDGDLVYKK"
     gene            4845..6245
                     /gene="yfcC"
                     /locus_tag="RD444_002687"
     CDS             4845..6245
                     /gene="yfcC"
                     /locus_tag="RD444_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDT7550002.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            6278..6445
                     /locus_tag="RD444_002688"
     CDS             6278..6445
                     /locus_tag="RD444_002688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550003.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            6499..6755
                     /locus_tag="RD444_002689"
                     /pseudo
     CDS             6499..6755
                     /locus_tag="RD444_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         2164 a         1043 c         1420 g         2128 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaatgtttc cacagaggac
     4441 ttctttaatt attgtcgatt agaggaaagt ggtgtaacag tcactagcgg tacagattta
     4501 ccgctgttta ttactgatat tcctgaatcg attataagag cagtattccg tcgttttcct
     4561 gaaggagaag aagtctggga gaaagaaaca agcttcacga caaccgagct gctgaaaagt
     4621 tttacaagca acagcttcaa agtaaatggt ttatcggagt acggagaaat aaaagtaggg
     4681 aaaagcgcca gcttagctgt tttcagcgag gacctctgta ctgatgactg gcaaaaagtg
     4741 atggctgcgg aagttatcgc tacctatctt gatggtgact tagtttacaa gaaataatgc
     4801 ttttcgatat aacgttataa aacgaattga agagaggtta aaaaatggaa acaatgaaaa
     4861 gaaaattcac cttcccacat agttatacat tgatttttat cttaattatt ttggctgcaa
     4921 ttttaacttg gataattcct agtggtcagt ttgagacagt tgttgaaaac gtcgcaggta
     4981 tggaaaaaac aacggttgtt ccgggaacct atcaccaaat cgataaggca gattatggac
     5041 aaggtttttc ctctatacta atggcaccag ctgaagggat tatttcagct gttgaggttg
     5101 ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat cctaaaaacg ggagccatcg
     5161 atcgagggct gtttacctta gcaaaagttc tgggagacaa ggggatctta gttattccga
     5221 tgtcgatgat tctattcagc ttaggaggat caacctttgg catgagcgag gaagtcattc
     5281 ccttatttgc ggtgtttatt tctctgatgt tttctcttgg ctatgattcc atgactgcga
     5341 tcttgattct atttttaagt acacaagcgg gatatgttgg gtcgacgatt aatcccttca
     5401 acgttttaat cgcacaggga gtagctggcg tttacggaaa tcccctgctt tggtttcgga
     5461 tgctatcctg gattgtcatt actgggattt caattgtttt caccatgctt tatgctagca
     5521 gagtcaaaaa gaatccactg tcctctattg tttatgagag tgatttaaag gttaagcata
     5581 agtttcaaaa ttttgacact gaaaaagagt tttcaacaca agataaacta attatcgccg
     5641 gttttatcgt aggtttagga atcatgattt ggggtatcat ttccaaggga tggtatatgg
     5701 ttgaaatttc tgcactattc ttaggattag gtctttttgc tgggggcatc gctagatttt
     5761 ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc agatttcgcc tatgctgctg
     5821 taatcattgg gctagctaga ggagtcttgg taatcttgga aggtggaatg attattgaca
     5881 ctattctaaa cagcttggct aaccttttag gtggtcttcc aaaattcctg ttcactacga
     5941 ttctactcat ttctcaagta attgtttcct tttttgttcc atcctcctca ggagctgcgg
     6001 cattaacgat gccggttatg gcaccattgg gtgatctaat gaatgtaagt cgtgatgttg
     6061 ttgttctaag taaccagttt ggaaatggtt taatgaatat tatctcccca actagtggtg
     6121 tattactggc aggattagcc atcgcagaga ttaatttctc taaatggttt aaggttggct
     6181 tcaaaatatt tgccatctta acccttgttt ctgctgtttt gttatacatt gctacactct
     6241 tttagtctta atcatccatt aataggaggc aatagttatg attaaaataa atcaattagc
     6301 aggaatgaat atccactatt tattttattc tctcaaagaa tttttcgctt atcaacagaa
     6361 aattggtatt tcttctgttg aactttgggg aggtgctccg cattttttca tagatgcttt
     6421 tactggttct gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag
     6481 atagcaggag gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt
     6541 attgtagccg tgggctacta tcttcgttat aaccttagct atcgtgaagt tcgagaaatc
     6601 ttatatgatc gtggcattaa cgtttctcat acgacgattt atcgttgggt gtaagaatat
     6661 ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa
     6721 atggatgaaa cgtacatcaa aattaaagga aaatg
//
LOCUS       DAPBAB010000105         6731 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPBAB010000105 DAPBAB010000000
VERSION     DAPBAB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.113"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..241)
                     /locus_tag="RD444_002690"
     CDS             complement(<1..241)
                     /locus_tag="RD444_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7550004.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
     gene            544..1818
                     /locus_tag="RD444_002691"
     CDS             544..1818
                     /locus_tag="RD444_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7550005.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1872..3299
                     /locus_tag="RD444_002692"
     CDS             1872..3299
                     /locus_tag="RD444_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDT7550006.1"
                     /translation="MTKKAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            3312..4184
                     /locus_tag="RD444_002693"
     CDS             3312..4184
                     /locus_tag="RD444_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7550007.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            4279..5274
                     /locus_tag="RD444_002694"
     CDS             4279..5274
                     /locus_tag="RD444_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7550008.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            5980..6597
                     /locus_tag="RD444_002695"
     CDS             5980..6597
                     /locus_tag="RD444_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550009.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTPKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT         2332 a          935 c         1298 g         2166 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg
      301 gcttttttat ttgtctcgct taattataca aacggcgtta taaaaaatag tgccattaaa
      361 aaagaaaagc gacacaccaa taaaagtcat atcctgtgac attatatggc attatccaaa
      421 tagaataacc ggttgacatg taagcggatt tcttttataa ttttaggagt acaagtcctt
      481 ttctaaaaat gttaaccggt tatcatgaaa ggggatacaa atatatcgtt ggaggataaa
      541 aatatgtcaa aagttaacgt atttaaaaat caatcttatt tacaaagttc agctacatta
      601 ttactatttt ttgcttcttg gggtgtttgg tggtcatttt ttcaactttg gctaacatct
      661 gaatcaaatg gtttagggtt atctggcagt gctgtaggaa cagtattctc ggcaaattcg
      721 ttagttacct taattttgat gtttatttat ggaacattac aagataaatt gtatattaaa
      781 cgaaatttat taatttttgc ttctgtatta gcgacacttg ttggaccatt ttttatatgg
      841 atatatgggc cattgctaga taacaatttt aatttaggca ttattatggg agcgctattt
      901 ttgtcagctg gatatttagc ttctgtagga gtttttgaag ctgtgtcaga aaggtttagt
      961 cgtttatttg gctttgaata tggacaagca agggcgtggg gatcatttgg ttatgccttg
     1021 gtagcgcttt tggcaggatt tttatttgta aaaaatcctc atttaaactt ttgggcggga
     1081 tctttctttg gttctttact attgttaaat ttattatttt ggaaccctaa agttgaacgg
     1141 gaagcaaatc aaaattttaa tcaagaacaa gctgaatcaa atagtattcc ttctttaaaa
     1201 gaaatgtttg atctaatgaa actgcctcaa ttatggacga taatcatctt tattgttttt
     1261 acatggacat tttatacggt attcgatcaa caaatgtttc cgggatttta tactggtttg
     1321 ttttcaacat cagctaatgg tgaaaaaata tatgggacat tgaatgctat tcaagtattt
     1381 tgtgaagcgt taatgatggg aattgttcca atcattatga gaaaattagg ggttcgaaat
     1441 actttgttat taggtgtaac cattatgtgt gtacgaattg gattgtgcgg gtttgcctcg
     1501 acaccattat ctgtttcatg cataaaaatg ttgcatgctt tagaagtacc attatttaca
     1561 ttaccaatgt ttcgctattt tacacttcat tttgatacaa agctatcagc aaccctctat
     1621 atgataggat ttcagatagc tgctcaaatt gggcaagtga ttttatcaac accattggga
     1681 atattaagag acaacgttgg ctatcaacca acatttaaaa ttatttctct tattgtatta
     1741 ctagcaggca tatatgcatt ctttattctt aaacaagatg atagagatgt tcaaggggat
     1801 ccatttattc gaggataatg gaaaccaaca gaataaaaaa caattttatc tagaaagtga
     1861 gcgaatatat tatgacaaaa aaagctatag aattaacaaa tacacgttat cgtttgggat
     1921 atcatgtgtc tgcaccatca ggatggatta atgatccaaa tggattttgc tattttgatg
     1981 ggtattatca tgtgttttat caacatcatc catatagtgc tgaatgggga ccaatgcact
     2041 gggcacatgc tagaagtaag gatttagttc attgggaatc tttaccactt gctttgactc
     2101 ctggagatca agaagatgaa ggtggttgtt tttcaggaag cgctattgaa aaaaatggtg
     2161 tgttgtatct gttctataca ggacatcact attatggaga caatgatcca gatcattttt
     2221 ggcaaaatca aaacatggcg tatagtacgg atggcattca ctttactaaa tatgaaaaaa
     2281 atcctgtaat agcaaaagca ccagaagata acacacatca tttcagagat ccaaaagtat
     2341 ggaaacacga tgaaaaatat tacatggttt tgggtagtca agaaaaagat ggattaggtc
     2401 gtgctattgt ttatcagtcg ttagatttat taaattggga ttacattgga ccaatttcta
     2461 aagcgaagga agtacttaca gaaggattta tgtgggaatg cccagacttt tttgaattaa
     2521 atggaaaatc tattctttta ttatcccctc agggaattga agcaaatgga aaagattatt
     2581 taaatctata tgaaacaggc tactttatcg gtgattatga ttatgagaca gcaaaattca
     2641 ttagaggaaa tttttatgaa ttagataagg gacacgattt ctatgcaaca caaacaactt
     2701 tatctccaga tggtagacgt attgttatgg cctggatgga tatgtgggaa agccctatgc
     2761 ctgaaaaagt agacggatgg gcaggagcat taacgcttcc tcgagaatta gagttaattg
     2821 gagatcgttt gtatatgaaa cctgtgagag aattagaaca acttcgagtt ggcagcggcg
     2881 ttgaaacgac agttaatttg gcaggagaaa taataatcgc tcaagatgct tcattcaaag
     2941 aagctctaat tgatgtacta ttgactaaat caaaaaatca agaaatcaaa ttttcattaa
     3001 aaacaaaaga tgaagagtta atcaatattt tttattctaa ggataaaaat gaatgtacct
     3061 taactcgtaa cggcaaaaat gatccaaggt atggaaccat tcagatgtgt gatagattat
     3121 cacttcgagt gtttatcgat aaaagttcgg cagagatttt tattaatgat ggcgagcttg
     3181 tttttacaga gcgttattat acagaaaatc aaccaagtat gcatttgagt gttagtgaag
     3241 aagcttttgt ctcgagtaca atttatcatc taaaaaaaga tgcaatttct tacgagtaag
     3301 gaggttttat gatgttaatt ggtagcatag aagcaggtgg gactaaattt gtatgtgccg
     3361 tgggagacga ggattaccga gtacaagacc aaataatttg tccaacgacc acccccaggg
     3421 aaacattgaa aaaaactgtt gactatttta aacagtttga tataaatgca ttaggtattg
     3481 catcatttgg tccaatagaa attcgaaaaa attcaccaaa atcaggatat attacgtcta
     3541 caccaaaggt gggatggcga aatgttaatg tgttaggtta tttacaagat gagctgaatg
     3601 ttccaatgtc ttggacaacg gatgtgaacg gatcagctta tggagagtac atcatgtcca
     3661 ctttatcgaa tgaaaaaata aattcattag tttattacac cgttggtact ggcgttggtg
     3721 gtggagctgt tgttgacgga aaactattag gatcgtttgg tcattcagaa atgggccata
     3781 cgtttgtaaa acgccatcct gatgatattg attttaaagg aatttgtcct tatcatggtg
     3841 attgtttaga aggcttagtt gcgggaccaa cgtttgaagc acgttttgga aaaaagggtg
     3901 aagacgtttc tttaacggat cctatttggg atgtaatggc ctattatcta gcacaagctg
     3961 tgattcaaac aacattgatt atacgaccgg ataaaatagt atttggtgga agcgtagtaa
     4021 atgaaaaatt gttagaaaaa attcgtgtta atgttaataa attattggat gattacgtgg
     4081 aaataccacc gttaagtaaa tatattacgc gtcctttaat taaaaataat ggatcggcaa
     4141 cactaggaaa ctttgctttg ggcttgaatc aattacaaga ataaaacata gaaaaaatat
     4201 cgattatcag gtatagtagt tctatagagt tctttttttc gatagttaat tattgcatta
     4261 tttaggagag acaagagaat gcgcccaaaa ttagaagatg ttgccaaaag agcaaatgta
     4321 tcaaaaacaa ctgtttcacg tgtattaaac aatcgtggct atttaagtca aaaaacgatt
     4381 gataatgttt ataaggctat agaagaactc aattatcaac cgaacgttgt agcgcgtcaa
     4441 ttatttcaaa agaaaactaa cattgtcggg ttactatttc ctacagtagc caatcctttt
     4501 tttagtgaac tcgttgaggc attggaaaaa aagttatatg aaattggtta taaggtatta
     4561 ataggcaatt cgatgaataa taaagaaaag gaaacaaatt atttaaatca gttattgtct
     4621 gatcaagtag atggtttaat tgttggtaca cataaccaag gaattcagga atataaatat
     4681 cagaatttac cgattgttgc aattgatcga gtgatgaatg aagatattcc agttgttgaa
     4741 tccgataact acaatggtgg aaaacttgca accaagttat taattgcaca aggtgcaaaa
     4801 aatattattc atacaaacgg tccaattgac ttacagactc cggctaatcg tcggagacta
     4861 gcctatgaag atactatgaa agcatatcag ttaattccac gaactgtgac attagatttc
     4921 aatatttctt atgtaaagaa aaaacagatt tttttccaaa tgtttgagga ttatcctaaa
     4981 atagatggta tttttgcttc aaatgacatc gatgcggccc taatattaca agtagcaaaa
     5041 gaaaaggggt taaatgtacc agctgattta ttagtagtcg gatatgatgg tactttaatg
     5101 actcggagta tattaccaga tctgacaaca gtaatacaac caattaatga tatagcagac
     5161 acagctgttg ctattttgat gaaacgaata aataaagaag aaacaaaaaa ggaatatatt
     5221 ttacctgtca cattatggat ggggaagaca tcagtaaaga aaaagaacaa ttaaatttat
     5281 aaagactatc aatgataatt tcattattaa ttcgtgatat aaaaaattgt taaatctctt
     5341 ataatagact atgatacaaa atcatgcttt taatctgtgt agatgttgcc aaatatttga
     5401 aagacataga acttgtcaga aaaaccttaa caattcctca atgggcggat aagctaggca
     5461 aaagggctgg cataaatttt tcagtccttc taacagaatc aattgcagat aaagcagata
     5521 acattcttcg ttctggaaga aataattaga actcaaaagg gtcagaaact taaaagtttt
     5581 tgatcccttt tttattcctc atttcatagc ttttttattc ctcatttcat agctttttta
     5641 gtagctcaat taattaggtc agaaagttac ttttttaaaa ggacaccaga gtataccctg
     5701 ttgggctctt ggtgttctat tgaatgtaaa tgagtgtcat ttttatattt aattggcaca
     5761 gttttttcta attatttgtc atattcatag tcaactatag ctacaaaagc aactaatttt
     5821 ggagatagat tgagatattt aaaaatctat catgaaaaat tagttgcttt tgtcacttag
     5881 cgctgaaaat ttatgccatt tatctagaaa aatgacaaca gagttgggca acagaattta
     5941 aaaatgatcc ggatttcaga attttaaagg agttttatta tggcgaaaat tgggtatgct
     6001 cgagtaagca gcttagatca aaatttagac cgacaattag aattattatc agagtgtgat
     6061 aaaattttta gtgataaagc aagtgggaaa gataccaatc gagaaggatt tcaagaaatg
     6121 atgaaattca tacgtgaagg tgatattgtc gttgtgacag aattaaaacg acttggacga
     6181 aataataaag agctaacaga aacgatgaat ttaattcagc tgaagaaagc tacactggaa
     6241 gttttgaatt tgccagcgtt aacaggtatc actgatgata atttacgtcg attacttaat
     6301 aacttaatta tcgaactata caagtaccag gccgaagaag aacgtcggta tattcgcgaa
     6361 acgcaacaac aaggaattgc actcgctaaa gcaaaaggga aatacaaggg aggtacgcca
     6421 aaatatcgtg agaatgatcc gcgacttcaa ttagcattta atttgttttt gactggatct
     6481 acagataaag aagttgaaga gctaacaggg attaatcgta gaacgtttag aagatatcga
     6541 atgaaacatg gtattactaa tgaagttagg gagcaaaaaa ataaatcttt cagttgaggc
     6601 taatataatt ttgcaagaaa gggattttta tgaaagaaat tagattttca aatcaaaata
     6661 tgcagaatta ctataatgaa attatgaata tggaaactag tgaattgaat ttaattatag
     6721 ccgaaaaaaa a
//
LOCUS       DAPBAB010000106         6549 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPBAB010000106 DAPBAB010000000
VERSION     DAPBAB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.114"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..184)
                     /locus_tag="RD444_002696"
     CDS             complement(<1..184)
                     /locus_tag="RD444_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550010.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDS"
     gene            complement(200..952)
                     /locus_tag="RD444_002697"
     CDS             complement(200..952)
                     /locus_tag="RD444_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7550011.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(967..2943)
                     /locus_tag="RD444_002698"
     CDS             complement(967..2943)
                     /locus_tag="RD444_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7550012.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(2996..3667)
                     /locus_tag="RD444_002699"
     CDS             complement(2996..3667)
                     /locus_tag="RD444_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDT7550013.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(3749..4915)
                     /locus_tag="RD444_002700"
     CDS             complement(3749..4915)
                     /locus_tag="RD444_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDT7550014.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKSELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(4921..5610)
                     /locus_tag="RD444_002701"
     CDS             complement(4921..5610)
                     /locus_tag="RD444_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDT7550015.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(5737..5976)
                     /locus_tag="RD444_002702"
     CDS             complement(5737..5976)
                     /locus_tag="RD444_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550016.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(6063..6278)
                     /locus_tag="RD444_002703"
     CDS             complement(6063..6278)
                     /locus_tag="RD444_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550017.1"
                     /translation="MAKSVLEPINMDSLTKGKYIQLLARGYTPRDVFRSLAEGTNMKK
                     STLRKEFDYWRIQNGIKELKPVRPVVK"
BASE COUNT         1927 a         1311 c          986 g         2325 t
ORIGIN      
        1 aagaatcaac tgttgacgaa tcgacggttt catcgctgct ttgaatcgtg ctgctatctg
       61 acgtttggat tgtttgttct gtgctatccg tgctttcttg tctgtctgca aacgtcaact
      121 gtgtgtaggc agtgaaagaa aaaagagcgg ctgcacaaac aaatagccat tttctatttc
      181 tcatgttggt taccctcctt tattttttct ttttataaac aaatagcgaa ccaatgatca
      241 tgactgaaag aaaaacagta cttcctaaaa gccatttcca agataatttt tgtttcttat
      301 tttcttcatc ttttaattct gattttgtaa aggttactcg cttcccatac actaggaaac
      361 ggtgtgtgtt gatcccctta ggcgtacatg tcattagcgt tactcgatct tcattgggat
      421 ttggttctaa gtaagtgaca aagtttcttt gtagttcgtc tttttgcact tcctcaatcc
      481 gatcgatttg ataagcgtgc tgctcaccat caactttgat ataaaacttt tctccctttt
      541 ttactgaagg taaatcatca aaaagattat ctttataaag tccactatga cctgcgatta
      601 aaggattttt accgtttcct cctgtaatat caccagttcc ttccgtaata cctacaccat
      661 tttctaaaat tttgtcggac gttccgtcat aaattggtag ttttaaagaa atacttagaa
      721 ttgtaagaat accaacagga tcgcctaact caatcttagc aacatccaaa gaagaatccg
      781 tttctgctgc cgttcctgtt cgatcgattg cggcaatctg ctgattttgt tggtatttcg
      841 ccacgctcgc ttcagcaggc atcaaaagta agacacctac agctgaaagt aaccagaaag
      901 caatgatcca tttacccatg attcgctttt tcttaggctt cctgataatc attggttttg
      961 atccttctag gctttcttct taaagtaaac aactgcagaa agtgcccctg ctcctagcaa
     1021 caagcaagag attaatacat acgtttgaaa accgttgcta ccagtcattg gtacggaagc
     1081 tttttgatta tttttgatcg tgattcggtt ttcttctttg tatgaatctt tgtctacttt
     1141 gaaagagatc tcttttgtta atttttgata gttttcagga gcttttgttt cttccagtga
     1201 gtaatctcct tcagttaaac cagtgatttc aaaatgaccc tctttatctg attttaggat
     1261 cgctgcattt gctacatcgt ctacccatac tgcttctttg acaccgtctt tatccgtatc
     1321 aactaatcca ccataatatt ttccttctgc gtttttagca gcaaattctg cgccttcaag
     1381 cggtgttgct ttgattcctg ttccaaatag tccgctatcg acttttaaga attttgcacc
     1441 ataagtcaca acgtcagctt tcgttttttc gtggtgttct tcctgattaa atggattgtt
     1501 gttataaacg aaatccacgc tatttgttaa tggattggca acagctgcgc tgtcatttac
     1561 atacatgtta taagtcattt gaagatcttg tccgtgtaaa cttgctacag tatcacttac
     1621 ttggccatta ttcacgataa aatcgatcac aaagccattt tctgaatttt ggattttata
     1681 atctgtattt tcttttaata ccacagatcc atctttattt gttactttga tatttgcttt
     1741 agcagcgaca taagaaagtc cttgtcctgc tgtatccccc catttaaata cactgtaagg
     1801 agaagtttcg cttttcactc cgtttactgt gataccgtct gctccgcctg caatgtctgt
     1861 tgggatatga attgttcctt gataatttac tgcgtcacca atttggaaag aatgttcttg
     1921 tcctgttcca tttgttactg ttttatcgat cactggtgct ttatagttta cgtatgaagc
     1981 ttttaagtca cttgtcacgc catcattcgt aatttcaaag gttaatttat tgtgcgcatc
     2041 gttgcgagcg tctgcgccta ataaatattg atctgctgtt ggttcttgat ctgatccaac
     2101 tacaacttct gaaggaactt ccacaaagta gtattttcct aacgtcaaat cagttgcagt
     2161 cagctttcct tgatcatccg atacaagctc tcctaatttt gttccttttc caggttcgcc
     2221 cttgtataac tcaaattttg ccccttttaa aggtgtttgt ttgctttgag ctgtgccatc
     2281 gtcaccaaat ttcgttaatt caaaactcaa cttagaaaca atgttctttg gatacaaatg
     2341 aatatctttt aaaaatcctg atcctgtgtt gtctgtcatt ggcgcaatca caaccattgg
     2401 ttttgctttt tgtgtgacta atccagcagc ggatttactt tcataaacca catatacatt
     2461 ccctttggaa ttgacatagg ctggttgatc agcaaaagtg atctcaccat tttcatctac
     2521 agctgaagta attttattgg ttgcattctt aacgtactca ctgttagatc ccttgtcttc
     2581 tacatcttta acgatttcgt ccactttcgc gttggttaga tcagaatctt cagtcggtaa
     2641 tacttgatcc gtaatatttc ctaacgtaaa ctcaacatca ccataatctg ccttgttgta
     2701 tttcgttaca cctgtaggat attgatcgtg cgtataacca tcatttttga ttccgtcaat
     2761 atcaacgttc aattgcgtac tttgatcgta cattaattta tgaaccgtga tattgaccct
     2821 ttctggtggc gttttttgcg tgacatcttc tgcataagct cgatacccct gaaagcagcc
     2881 tgcaatgatt cctaacattc caacaatcat tccagctttt tttacatacc gatttattgc
     2941 cattttttac tctcctcatt tcatatttca acgactcgcg ttgattaaat tcttgttatt
     3001 gtagctctcc ctgtaccacg tatctttctt ttccttgtga cgtataaaca caagtaaaca
     3061 acgttaataa gggtttctct cctttctttg gtaaagacaa gaaagagttc tcttcaggaa
     3121 cgccatcgat taatttaaag tactcagata cgataaattt acgcgttacc ttgtaacgat
     3181 agacgccatc gttactaact aagtccatca cctcccctaa tgacaacttt tgaatatcag
     3241 aaaataaaac acctggcatt tccatgttat gacctgctag gacatagttt cctttaccca
     3301 tttcggcatc ttcataaaaa taagtagccg ctccaagact caaagtgtat tgatttgccc
     3361 ctttaaaaat tggcaatgct aaaccaagtg caggaatctg aatttgtgca aatacttgtt
     3421 tattgacgcc catttcgatt gcttcttttt gtatggacaa catttgcccc aaagacatat
     3481 cattattgac gttcgttgca tcctccttat aactctttgc gtgtgcattg atttgatcta
     3541 cgggcatctt tttttctata ttttgcttaa acacctctcc ttcatgtttt tggtagctaa
     3601 catagccaaa cacactgcaa acgacgatta aagcgactcc taaaaaacca aataaccatt
     3661 ttttcatcca tctgcctcct ttcagacaaa acaaaaaaca ctttttcaca acgaaaaagt
     3721 gttttctctt attactattc tatttcattt atctggtggt tttatattgg tgtgtagcca
     3781 ttgctaacat taagaataca attggcaccc aactgattgt cgttaatgtt tcttccatga
     3841 acgattgaca catgatcgct aaaaaagccc acaagaatgt tagttgtcca tttgtcagct
     3901 cactttttct tacgagcaac cataagaaac taagcaaaag aataatcgat agaacaaaac
     3961 caatcaatcc atagtgattg acgagtgcgt gatacgtatt atgattacct agaatctgat
     4021 ttcctctatg gaaaaagaaa ggtttcatcc ctacaaatat ttgtcctgtt ttttcgccta
     4081 atgtttcata gaatttgcgc caaatttctt ctcgtcctgt aaataagtga actttgtctg
     4141 acattgcagc aaaataagaa atcggtaaag ctagaaccga aactgctgca tacatccaaa
     4201 aggacaagtg tgtatttcga aaaaccttct taggtaaaat ttctaagata atgaagataa
     4261 caagcgcata taatgctgct ttcgattggc aaacccaaat cgttgcccca gcaagaatca
     4321 acgctggtaa tgatagcaat cgcatgtacc attttccaaa tgaactagca aatcctgtga
     4381 taagaagggc taatgtcatt aaagaactac ctatcgtatt tgtattgatc caaatatctg
     4441 ataatttgtt cgatggattc caaatacttt gtccttctgg tactggtatc tcagtaaata
     4501 ctcgataaac cgttacgagt aaaaagacat agaatactac aagatacaag ctacgatcaa
     4561 ttacagtaaa tttcgtccat tttacaatca aaataatagc aataattgtc ggaacgatca
     4621 gatcaccata gaaagtatct cctcggtagc ttctaaaaaa agaaaagaaa aggtaaaaaa
     4681 gaatgcctgc tgcaatgaag aatagatcgt ttctctttac cttgtacaga ttcaaaagaa
     4741 acaaacagcc tacgaaaaac gtcactaact gtgcagcatt atcataaata aataatatcg
     4801 taggaggatt taaccccacc gtccatatta aatatagaat ggataccaac agtaacagaa
     4861 aaatggcact cattttattg atcaattttt ctacaagttc ttgaatgtga ctcacttccc
     4921 ttatttattg attttttcta cattgactaa ttcacactgg atcacatcgc actttttcat
     4981 attatttgct tgaaaggcta acagtgtgag tagtggtttt ttacctgctt ttggcaagga
     5041 taaaaaggaa ttgttttcct tagattgaac tacctggctg taatcagaaa tttttcctct
     5101 tttggagact tcatactgat acttgtaatg agcatcgatt agatcaattt cttctccttc
     5161 agataaatca gtcatatcgg ataataaaac gcttggtgta tctacgttat aaccagcaag
     5221 aatataattt ccatttccca tttcagtatc tagataataa gtagaagcta ctcctaaact
     5281 aagcgtgtcg ctattcgctc cttgaaaaac agggagaata aatcctgtag aagaaaaaac
     5341 aagttttcca gcaatttcac ctttgagatc cttttctaat gcctgttttt gattagtaat
     5401 cagttgtctc ttagaaatat ccgttaaact ggttaaatta tcttgatagg ttttagcctg
     5461 ttcctgaata tggattgttt ttgttacacc atctactttt tcagcgattt tgtccttttg
     5521 ataaccttga taagcaaaat aactaaaaag aagaaaaaca atgattacgc ccacgtaagg
     5581 attaaataaa gttttattgt ttttattcat ctatctacct tctatatatg aattaattta
     5641 cctacatcta accaagataa accatgttaa attttactat tctttttatt caataacaat
     5701 gccacatcat aattgatcat tttcatatca taatcgtcaa gaaacttcta ataatagttg
     5761 gcaattggaa cctgttttta cataagttcg cctcaactgt tcaaagtttt tcattgaacc
     5821 taacaaacaa gctttctctt ttcctgttat cttcatatca ttgatatatt gcatgacaga
     5881 taatctttta aggtgttttt gtttcatcat ttttagcaac ttttcttcca tttctttcga
     5941 cacttttttt ctgttttcga tttcttcata attcatgagt tttcctacct tccttgtttt
     6001 ttgcaaaaat aaaaaagcct atgcttacat acgcctttga tttactctag ttattatatt
     6061 tttcatttga ctactggtcg aacaggtttt aactctttaa ttccattttg aattcgccaa
     6121 tagtcgaatt cttttctcaa cgttgatttt ttcatatttg ttccttccgc tagggatcga
     6181 aaaacgtctc ttggagtata acctcgagct aataattgaa tgtactttcc tttagttaaa
     6241 ctgtccatat ttattggttc taaaactgat tttgccattt cttacctcca ttatccaaaa
     6301 cattcaattt acagtaataa caaaataaaa tattatatct ataattaaaa atgcaaaaca
     6361 cacgattaaa ttcaatagca ataaataaaa aaatgaacta tttaagcttt taaaaaagta
     6421 tcttttaacg tcttttatat caaatttata ctcaatttta aaagctttgt tgaataaaaa
     6481 aatagatagt tttaccaacc agtagaaaat aaaacaatga agatatgcct ggaattctgt
     6541 aagtaataa
//
LOCUS       DAPBAB010000107         6133 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPBAB010000107 DAPBAB010000000
VERSION     DAPBAB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.115"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(207..1169)
                     /locus_tag="RD444_002704"
     CDS             complement(207..1169)
                     /locus_tag="RD444_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7550018.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="RD444_002705"
     CDS             complement(1171..2610)
                     /locus_tag="RD444_002705"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDT7550019.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="RD444_002706"
     CDS             2795..4741
                     /locus_tag="RD444_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDT7550020.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="RD444_002707"
     CDS             5005..5877
                     /locus_tag="RD444_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7550021.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1946 a         1054 c         1084 g         2049 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 atatctttgc ttc
//
LOCUS       DAPBAB010000108         6114 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPBAB010000108 DAPBAB010000000
VERSION     DAPBAB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.116"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(125..772)
                     /locus_tag="RD444_002708"
     CDS             complement(125..772)
                     /locus_tag="RD444_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDT7550022.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1180..1683)
                     /locus_tag="RD444_002709"
     CDS             complement(1180..1683)
                     /locus_tag="RD444_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7550023.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1806..2570)
                     /locus_tag="RD444_002710"
     CDS             complement(1806..2570)
                     /locus_tag="RD444_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDT7550024.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2678..2953
                     /locus_tag="RD444_002711"
     CDS             2678..2953
                     /locus_tag="RD444_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDT7550025.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            complement(3154..3501)
                     /locus_tag="RD444_002712"
     CDS             complement(3154..3501)
                     /locus_tag="RD444_002712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550026.1"
                     /translation="MIPSVSFLFKKLKTFFILPEIEESNELTASDRSAFLLPVFNTWG
                     KAPQKQPYRRCSKVSFLVLRQIYAKMWKAIFTKFSLILKTKRLFHNQSLMLDADQLFH
                     NLIDGVLSAASRY"
     gene            3669..4598
                     /gene="yidC"
                     /locus_tag="RD444_002713"
     CDS             3669..4598
                     /gene="yidC"
                     /locus_tag="RD444_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDT7550027.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            4701..6049
                     /locus_tag="RD444_002714"
                     /pseudo
     CDS             4701..6049
                     /locus_tag="RD444_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         1901 a         1167 c         1123 g         1923 t
ORIGIN      
        1 aaggggctgt ccccagactt tagtaggcgt cattagctat ctttcgttag catttaaagg
       61 cgaacaccat cacctattca cttccattaa aagatagcat atttttctgt aaaaaacaat
      121 aaaattatcg ttctttcttt acacgttcag caaattcata gaaaggagcc aatttcgtta
      181 actgttgatt acattggtca ctatcatgac aaatataatt cccctttttc gtatacgtgc
      241 catcagaacc tgctttcgtt gtagaaagaa aaagcgaaac attcgatgtt ttctgacaga
      301 ttgcacagat tcccttattt atcgttggtg ataaagtacc gctaatccct tttaattttt
      361 catcttcgta atagaccatg aattttttct gcgtacctgg atcattccag ccgatgtacg
      421 tatgttcttg caagtcgatt tcattccaag ctggttgttt gagttttttc acttttcgga
      481 ataatttctc gatttgtggt ttgctaggct gcataaacgg ctgaacgatt tgcttcagct
      541 cctccaatgc tctttctgct tgcgcttttg tcagttttac gtctaaaact gtcgttaaaa
      601 atgtatcgat ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcctgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
     1021 cccttgtttc gaatgaatgg tgttcaaaag cttgcatctg cagtaataag ccgaaacttc
     1081 acaaaaattt gagaagtaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     1141 tgtcacaacc tcaatatggt ctattattgg ttttacctgt taaatcgagg attctttccc
     1201 aaagtaacgt tgccatctct tacgcatact tgcagacatg ggctgagccg cttctttgac
     1261 tgcgcagtat ttgtccacga atgtcacaat atatccttct ttatacttag gcggtgcgat
     1321 cgttgcaccc cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat
     1381 tttttctgca ttctttacag caattcttgg atggatatac gcatgtgtcc cctcgtcaaa
     1441 tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc
     1501 tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga
     1561 atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc
     1621 tgtttctaaa agatcttgta cataagacat atattcttcg tcttcacgcc atttttctgt
     1681 cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat
     1741 accaccagtg ttcggattat tctagtcgct ctagataaaa tagctaaagc ttaatagttc
     1801 cttaatcata aagaaaaatg ataaagaacg atccctgctg cgactccgac attcaatgat
     1861 tccgcatttc ctttcattgg gatatatacg ttctgatcag tcaaagacaa gatttcctga
     1921 ctgactccct gaccttcgtt tcccatgatt atagcgaaat cctctgtttt ttctactttg
     1981 tctaatgcat gtgcttcttc atttaattct gtaccgtaca ccgtaaaccc tttgcctttc
     2041 attacaggga ttacatctgc caaatctttt tgtaacacag gcaaatgata attgcttccc
     2101 tgcatgcttc gcagcacctt tgtactataa atatctgccg ttcctttacc taataaaaca
     2161 ccagccatac caaacgcatc tgccgtacgg atcatcgttc ccacatttcc aggatcctgt
     2221 acattatcta gtaaaagcca gcctcctgaa aaatctggcg tatcttcaga tgttaacgtc
     2281 atcactgcta agatcccttg cggtgtaggt agatctgaca tagcggtcat gacctcatct
     2341 gaaactagaa aagtagaaat ctctttgttt acagccagcc agtctcccca ttctttcata
     2401 cctctctgtg tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcgatc
     2461 agatggaacc cttccaaaag atattctgcc ttttcttctc tgtatttctt tttatgtagt
     2521 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac attccttcac
     2581 ctcacctgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     2641 tcattgattt ataaaacagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaatgt
     2701 ccaaggacgt gtccagggcg tgggatttcg ctatatgacc aagatggttg ctgatcagtt
     2761 aggcgtgacc ggaacggtaa aaaacgaaga cgacggctca gtcacaatcg aagcaatcgg
     2821 aaatgacgac atcatccaaa aatttatcga agaagtcaag aagtctccga gcccaagtgg
     2881 acgagtgcag tatgtggatg tccaagaaga tccgatgatt gaggaacgaa agaaatttga
     2941 tgtggtggga taaggttgtg aaaaggtcgc ttagttttga gtattaagga aaattttgtg
     3001 aaaatggctt tccacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa
     3061 caccgtcgat aaggttgttt ttgaagcgct ttgcctcgag tattaaggag aaacattttc
     3121 aaaatagctt ctcatatttt gaaatgtaat gacttaatac cgagaggcag ctgataaaac
     3181 accgtcaata aggttgtgaa aaagctggtc tgcatcaagc attaaggact ggttgtgaaa
     3241 aagtcgctta gttttgagta ttaaggaaaa ttttgtgaaa atggctttcc acatttttgc
     3301 ataaatttgt cttaatacca agaaactgac ttttgaacac cgtcgataag gttgtttttg
     3361 aggcgctttg ccccaagtat taaagacagg tagtaaaaaa gctgatctat cacttgcagt
     3421 gagttcattt gactcctcta tttcgggcag aataaaaaaa gtttttagtt ttttaaataa
     3481 gaaagaaaca cttggaatca ctcttttctt tccaagtatt tctttcttga ttttttgtct
     3541 tttttcaaaa tttttcgcta cgaaaaagaa agaatattta tatgaaattt ctatgaagtt
     3601 gattgaattc catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga
     3661 agtattctat gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc
     3721 tctttttatc tggctgtgtg cggatggatt caaacggaaa ccctgacact tcaggaatcg
     3781 tttatcgagt actcgtccat cctatgggcc aagcgattac atatctagtt gagaatttca
     3841 attggtctta cggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc
     3901 cactaggtat cagtcaatca aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa
     3961 aaccacaagt tgaagcagca caacaaaaac taaaagctgc aacaacacgt gaagaacaaa
     4021 tggcagcaca agcagaaatg caacaagtat atcgcgaaaa cggattgagt atgaccggcg
     4081 gaatcggctg tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tattttacgg
     4141 ctcgctacac agaaggtatc cgtgaatcat ctttttacgg catcgatcta ggctcaccaa
     4201 gtatggtgct tgttgccatt gcaggggtcg cttatctgct tcaaggatat atttcaacaa
     4261 tcggtattcc tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta
     4321 tgatcgtttt catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtgggtg
     4381 gtgtgttcag ttgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag
     4441 cacaagtggc agaagagttg aaacaaaatc cgccaaaaca agtcgtcact ccaagaaaag
     4501 acgtgacaga atcggcaaag ccagctgaca ataaacaacc aaaaaacagt acaaacaaca
     4561 aaggccgcaa cgcaggaaag cagcaaaagc ggaaataaaa atatatgaca aaaaactgta
     4621 gacaaataaa aaaatttctt tgttctacag tttttttgtt acaatcaaag aaaacgtcgc
     4681 ttttcgagga ggaatgtata atgatgtcca aataatcaat ggatagtcga atggaagaag
     4741 ccaaaatttc gctagatgca cttgttccta aaaatcattt ggtccataaa attgacaaaa
     4801 tattgttatt tgattttatt tatccaattg ttgaatcaac ttactcgacc attggtcgtc
     4861 ctagcgttga tcctgtcatc ttagtaaaat tagtattcat tcaatattta tttaggattc
     4921 gttcgatgcg tcagtccatt aaagaagctg aaacaaacct agcttatcgt tggtttcttg
     4981 ggcttagttt aatcgataaa attcctcatt tttcaacgtt tggtaaaaac tatgtacggc
     5041 atttcagaga aaccgattgt ttgaacagat atttatcatt atccttgaac aagcagttga
     5101 agctagattt attcatgaag aaattttgta tatggattca acctatatta aagcgaatgc
     5161 gaaaaaacat atttttacaa gagaaatggt acataaataa gccattttat accaaaaaca
     5221 attgaaagaa aaaattaatg aagcaagaat tgcagaaggc aaacgtcctt ttggttcaat
     5281 cattcatcag gaggtggtag aagggaaaat tagtgtcgca gatccagaaa gtggttatta
     5341 tgtaaaaaca gaacaagaaa aacagtttgc ttattctgct cataccgttt gtgatgaaaa
     5401 tggttttgta ttagatgtca tgattacgcc tggaaatcta catgacagcc gaatgctcgt
     5461 gccacaaatc gaacgtgtta aatcatgttg tggtgtagct gctgatgctg cgtacaaaac
     5521 accttggaac gccaagtatt taatcgatcg aaaactacga cccatttttc cttatactcg
     5581 acccaaaaga agcaaagaac gttttaaaaa gaaagatttt tattatgatc cctattataa
     5641 ctggtaccta tgcccaaatg atcaaacact tcagtttcgc acaactactc gagatggtta
     5701 taaaaaatat gtatctaaat cttttatttg tgaaagttgt ctattattga aaaattatac
     5761 agaaagccag aaacaccaga aagaaataca tcgacatatt taggaagact acttagatga
     5821 agcagaacat ttacgccata cggtgtacaa tcgcgaaaaa tataaaaaaa agaaaagaaa
     5881 cgattgaacg cgtttttaca gacgcaaagg aaaagcatgg aatgcgctac accaaatatc
     5941 gaggcttgga aaaaaccagg tgcatacgat gcttgttttt gccgcaatga atctgcaaaa
     6001 attagccatt tggcagtggg ggcatatgtc tttatacccg tggtgctagt taattttttc
     6061 ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcatat agta
//
LOCUS       DAPBAB010000109         6101 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPBAB010000109 DAPBAB010000000
VERSION     DAPBAB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.117"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(57..284)
                     /locus_tag="RD444_002715"
     CDS             complement(57..284)
                     /locus_tag="RD444_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7550028.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(387..2975)
                     /locus_tag="RD444_002716"
     CDS             complement(387..2975)
                     /locus_tag="RD444_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDT7550029.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(2995..3654)
                     /locus_tag="RD444_002717"
     CDS             complement(2995..3654)
                     /locus_tag="RD444_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7550030.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3703..4206)
                     /gene="trmL"
                     /locus_tag="RD444_002718"
     CDS             complement(3703..4206)
                     /gene="trmL"
                     /locus_tag="RD444_002718"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDT7550031.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4427..5293)
                     /locus_tag="RD444_002719"
     CDS             complement(4427..5293)
                     /locus_tag="RD444_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7550032.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5402..6043
                     /locus_tag="RD444_002720"
     CDS             5402..6043
                     /locus_tag="RD444_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDT7550033.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
BASE COUNT         1666 a         1301 c         1113 g         2021 t
ORIGIN      
        1 tttttcacat cttctattca attgtcccgc aaaccatttc ctcacgattg aaagaatcaa
       61 ggattcgcag ggctgccagg attcgttgga ttgttcggcg tgttaggcgg aaaaataggc
      121 gccttgtcta acgttttttc tagttttgat tcttcacgat cgtctcgttc tacatcagga
      181 ctttcattca atttgaattc tttttccatc tcatctaact ttttaaagtc attgccagat
      241 tcttttgcta atttcgctaa tttatcttcg gtttctttag ccataaagta acgcccccct
      301 tttgatttca gcttagcata tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca
      361 tttttagcta tttctcatct ttaaaatcaa gcattttttg ccggtataaa atcgtaggga
      421 gaactgtttt ccataccaat catcggatct actaactggt tatcgatcac ttctgctgta
      481 agaacgaaat ctttagaaac agcaggttct acctcttctt tcttttcttc tccaacaaat
      541 gaagaaaaca gatctggttc cactgtttcc attacttggt ttttcgtttc attcgtccca
      601 ctgtcatctt ctacaggtag cttttctttt acaacccgtg gctcttttgt atagttccca
      661 tcttcaaatg gatcatttcc gaccaaagaa tcttcataag ctgctagttt ggtccggaga
      721 gtgatattca ttttatcatt atccatgatc cttgatttca acgtagtcat ccggcttgct
      781 gattcattcg ctacacaagt gacataggct tgctcttccc ctaacaaaat caacagttcc
      841 ttactgcgag tcactgccgt gtagagcaaa ttacgctgaa gcatccgctg atattgatgg
      901 accatgggca aaataaccat cttgaattca ctgccttgtg atttatggat tgagcaacaa
      961 taagaaagag tgattttatt ccattcgttt cttttataag tgacctcatt tgcatcaaac
     1021 tggataacta attcatccac tttatcttca gaatcttttg ccaagatgat acccgtgatt
     1081 tctcccatat ctccattgaa tacattcaat tcgggagtat tgactaattg gaggactttg
     1141 tctcctatcc gataaacggt gtcgttccaa tgtacttcct tttttccagg tttccctgga
     1201 ttgaatatct cctgcatcat tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt
     1261 ggtgctaata cttggatatc ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt
     1321 ttagagatca agggttcaat ttgataagca tcacttcgga aataggaacg atctttttga
     1381 ttctttgtga aatcttttgg cagacgtcct tctttgatct catgggcaag aggaataata
     1441 ctagaaccat caccttgccg ataaatctga ttgagttcca ttttgggaat ttgttcgatt
     1501 tccaaaagat catggagtac ttgaccagga ccgacagagg gaagctgatc tttgtctcct
     1561 actaagataa cttgcatatt cgtcggaatc gctttgaata atgtgttagc cagccatgta
     1621 tcgaccatag acatctcatc aacaatcaaa agtccgcctt ctaattcttt cgtactgact
     1681 gaaggtgttt tttcccgtcc attcagcccc agaagacgat ggatcgtact ggcaggcaga
     1741 cctgtcgttt cgttcattcg tttagctgct cggccagttg gtgctgcaag tagaatagga
     1801 aaaatttctt gggtatattt tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga
     1861 ttgacgattc cgttgatcac agtagttttc cccgtacctg gtcctcctgt taaaataaat
     1921 agaggggaac ggattgcttc agcgatagct tgttcttgcg aaggtccata ctgtatgccg
     1981 aatatttttt cgatacgccg aatattcttc tgcacttctt tttcaggata atggatttct
     2041 tttttacgac tgagaaggcg ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa
     2101 ctgttttcgt aaagattagt ctcttcttgt tgtatctttc cttcttcaac aagttggata
     2161 attacagtag caacctgatc aggctggatt tcaattgggc gacttgtttc taaaatacgg
     2221 atcgtttgct gaagcaatgt ctccgccggt acgtatgtat ctcctgtttg aactgcatgc
     2281 tgaaaaatct gatgcaagat agctgctcga attcttctcg gtgaatctgc tgcaataccg
     2341 atttgttcgg ctagattatc tgcacgtttg aacccgattc cttctatgtc ttcgactagc
     2401 tggtacggat tttcttcgat gatatccaaa gactcgttct tataggcctg gtagatactg
     2461 aaagctaact gactaccgaa cccgtaacga ttcagtccca tgatcacttg atccatcccg
     2521 tggtttagcc gaatcgtctc agcaagcatt tctctttttg gcttcgtcaa accggagatt
     2581 tgttttaata ccgaaggatc agcaatgatt ttttcgatag catcttctcc cagcaattct
     2641 acaatttttt ctgctgtttt cttcccgatt cctgggaatt tttcgctgga aagatacgct
     2701 acgatcccat ttgcagttgt tggcttttct tgctcatatc tttcgagttt aagctgcctt
     2761 ccgtatttag gatgatccac taattctcca taaaaacgat aagtctcatt ttcttgaata
     2821 tcgccgaaac ttccggtgac tacaatctct ttttcacggt aatcagctgt ggtttcactg
     2881 atgtgtacca aaagcacttt gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact
     2941 gtaccaacga aataaggcat ttcttctggc tgcatcggaa gccgcctcct tcttctatag
     3001 taaaggttct ggtgctgttt cagttcctaa aaaatctgtt tggttccata ctttcagttc
     3061 atatggcaat aatctgcaag gctcttcagt ctctaaaatc gtcaaactac tattgaacaa
     3121 tccacccatt tcacgcaact cacttagctc ttttcctgtc atccattgaa tggctgcagt
     3181 taaagaagct ccatgtccta cgaataaaag tgggccatct ttatgtcggg caaccgcatc
     3241 tgctacaaca gtttcgatac gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga
     3301 aggatcatac agatctaaat gattacgcaa attcggcaga ttatccggat atttctggta
     3361 catttcatcg atcgactgac cttctaaagt acccagccct agttctctta gcttatctgt
     3421 ataaaaaatc tctaccggac gagcaagctc tttgttgata ccttctgctg tatcacgtgc
     3481 tcgctttgct gtactggaat aaattgcttt aaaaggaaca tattttacct ttttcccaaa
     3541 tgctttgatt tcttcgtaac tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa
     3601 acgtctttcc tgattccatt gtgtcttccc atgtcgggta aaatatagtt ccatacgcag
     3661 caaactcctg tctttctttt attttactct ataataaact gtctaatcca gcttgtcatt
     3721 ttcatagtga tgcgtcaatt ctaaattagg gaatccttgc tggcgaagtg cttcatacac
     3781 tatcattgca gctgtattcg ataaattcaa tgagcggaca tgctcatcat tcatagggat
     3841 gcgtaaacac ttttcttcgt tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt
     3901 tccaaataaa aagaagtgct cttctccatc agcaaaattt tcgtcactat atactttatg
     3961 ggcaaatttt gagatcaaat gtagtttacg attacctaaa aatgttaaaa aagcttctaa
     4021 attactgtgg taagtaatat ttacatcatt ccaataatcc aaccctgccc gttttagatg
     4081 tttgtcatca gttgaaaaac ctagtggctc gattaaatga agcggagtat tcgtggctgc
     4141 acacgtacgg gcaatatttc cagtgtttgc agggattaat ggttcaaata aaacgatatg
     4201 attcatcagg atcattcctc tcatatttat gttcgtttct attttctgac aatttgattt
     4261 ttgcataaaa aaaaacgtat caactcggtg tcagtcactg cgagtcgtta cgttagtgaa
     4321 gctcttctca ctaactttta tcagaacagg aacctgataa aaaccaatga aaaaacaact
     4381 tgttgctaat ataacaccct caactttttt ttgcaacctc tactcattaa aaactgctag
     4441 aaaaacgttt tctcttagtt ccttttaata attctatgtc aattgtaatc ttcttcaaag
     4501 gattcttata taagtctgct ttgattcgct cttctacctg ccattctaaa ggtgacatgg
     4561 ccaaaagatc tttttgattt tcctctggta tctcaaattc ataggttaca cgctgacgtt
     4621 cagttaattt cattgcttca gcaaacttgg ctactacacg ttcattggaa taactctgtt
     4681 tttcaggttg attcggataa aaagcctgac gtagttcctt taaataattt tcttgtggca
     4741 ccaccttcaa cacttctcca tcttctgaga gtacacgttg gaattcttgg taatgagaag
     4801 gcgaaaagat atttaaaacc gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat
     4861 cagctacaca ccaaaatgct tcggcagatt gttctgtagc tgcgtagact ccttcttttg
     4921 aaatatcaaa tccaacagca ttttgaatct ttttcaattt tgaaagttca ttcaagaagc
     4981 taccttctcc gcagccaaca tctaatagcg agacttcttc aggtaaatgc tgcgcaatag
     5041 tctcaataac gggctgatac atgccagatt ggatcattct acgccttgga acgaacattc
     5101 cttgatcgta ttctgtttta accgcatggg ataaaaaata aagtgttcct tttttggaaa
     5161 ggtcaaaacg gtgattattt ttgcatatca agccattatc agactttttt acttcttctt
     5221 ggcaaaccgg acagcggaac agctgatgat tttctgccaa aaactggcgt cctcggtcaa
     5281 ttttttttaa catagttccc cctcatttct gttttatctt accgttagat gctcactttg
     5341 gcaactgcta aaaatatgat acaatgccta gaagaaagtg aaagataaag gagaagatta
     5401 gatgatcaaa gaattttgcg ctgaaaacta cacatctatt cctctagcaa ttgctagagg
     5461 agccgatcgg atcgaattgt gtgacaactt agcggtcggt ggcacaaccc cgagtactgg
     5521 tgtgatcgaa gaaacgttaa gttatgctgg ggaaaaagat atccccgtaa tgacgattat
     5581 tcgtcctaga gggggcgact tcgtctataa cgatatcgaa ttgaaaatca tgcatactga
     5641 tttgattgaa gcaaagaaat tagggacgga tggcgtagta tttggttgtc ttactccttc
     5701 aggctggcta gacgaagagg ccttagaact attgatcgaa acagcagaag ggttgcagat
     5761 caccttccat atggcttttg acagtattcc tgaagatcgg caattcgaag caatcgattg
     5821 gctggtagaa catggtgttg accgtatctt gactcatggc ggtcctgctg acaaaactat
     5881 tgtagaaaat ttcccacggc ttaaagaatt gattgcctat acagatggcc gcttaatcat
     5941 tttgcctggt ggtggtgtca ctgcagagaa tgcagcacat gtagctaaag aattgaacgt
     6001 aagcgaagta cacggaacca aaatcgtgga tcttcagtta taatcttata aacaaaaaaa
     6061 catttgcaac aggcataatg cttattgcaa atgtttttgt t
//
LOCUS       DAPBAB010000110         5962 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPBAB010000110 DAPBAB010000000
VERSION     DAPBAB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.118"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(229..504)
                     /locus_tag="RD444_002721"
     CDS             complement(229..504)
                     /locus_tag="RD444_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDT7550034.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(476..1429)
                     /locus_tag="RD444_002722"
     CDS             complement(476..1429)
                     /locus_tag="RD444_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7550035.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2041..3534
                     /locus_tag="RD444_002723"
     CDS             2041..3534
                     /locus_tag="RD444_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDT7550036.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3669..3965
                     /locus_tag="RD444_002724"
     CDS             3669..3965
                     /locus_tag="RD444_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDT7550037.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(3989..4948)
                     /locus_tag="RD444_002725"
     CDS             complement(3989..4948)
                     /locus_tag="RD444_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7550038.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5131..>5962
                     /locus_tag="RD444_002726"
     CDS             5131..>5962
                     /locus_tag="RD444_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550039.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     E"
BASE COUNT         1957 a          983 c         1075 g         1947 t
ORIGIN      
        1 aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc tcgtagataa
       61 gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa caccaaatgt
      121 tgtatacccc caaacaatac acatattttg aaggattagg attaataaag ctaaaaatat
      181 agtaagtata ctaaatatat ttactatatc tagtatactt actatatttt aatcttttga
      241 tcgtctatta tatataatcc gttcctcgtc gtttaaagta tcaacatagg cgtccattgc
      301 aagtttgact atttcataca taggaatatc ttttgcataa ctaagatccc gaatggtttt
      361 tagtacggga ggatctactt gaactggttt tctttctttc gcagtgtact ttcgttcccc
      421 taactcgaca ttaatatcat ctttaattga aatatcttca gcaccaattt tcttcttatc
      481 tttgttcgtt ggtaataatg ccatttagtt taatctcctt tccaagaggt aggatatgat
      541 tggccattga gtcagcatat ttaggtgtat aatcaaatcc ttcaatatca ccatgttgtt
      601 catagtattc aattctttct tctaattctg taaagatgtc tgcaaaaata gcccaccatc
      661 ttcgatctac ataatcattt aattggatac cattctcacc atacatttct aaccgatccg
      721 agcctttaac aatgctttta aaaatgtttg attcaccata cttatcaatt gttttttgat
      781 agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac aggcaaaatt ccaatgattt
      841 gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa aactttatta ataaaatctt
      901 ctgttccttc cattgcgtac cgttttaatt cttgcattgc aataatataa tctgcagcag
      961 ttaagaatgc acgaaccaca ttatctgttg atggtgggca gtcgataaag atataatcga
     1021 aattttttct caattcttct aaaggtttaa tgaaggccaa tgatctctta cgacgatcag
     1081 gatatttttc ggacaagtat tcattaagtt cttcagatcc aggagagcca gccataatat
     1141 ataaatgtgg acttacttga gtaatacctg aaacccagtc cccttttttg attgctgcag
     1201 acatggatcg atcaaagcta gtaatttgat acgttgatcc aatgttggaa gtagtatttc
     1261 tttgttggtc ataatcaatt agcaacacgt ctttttcaaa aagttgacta gaaacaatcg
     1321 ctaataaatc attgtctgta gtcttaccaa tgccgccttt tctacctgta agcattacaa
     1381 cttttcctag tttatctgat gttttaaatg cagtccgtaa gttttccata gttccctcca
     1441 atttcctata ctaaatatat ttagtatact tcatatactt aatatattcg atatatctaa
     1501 tatactccaa attgataaga attcagactt tataaggaat atagtggata tactaaatat
     1561 atttgctata ttcagtatat cagatatatt caaaatgtca aaatatattt gtgcgttttg
     1621 cgttgacaat ttagagtgaa aagaggtaaa cttaattcta tagaaaccac agataattca
     1681 ctatttacaa agtagcaaaa gcagtggtac tctttaaaca gaaaaattac ataaaaaaaa
     1741 gagacggaaa taattgattt tagcagaatc aattatttct gaatcacatg gttaggacat
     1801 gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa ctacttatat taaaagttga
     1861 tcggatacct tttccgattg cctttattct accaaaaaaa ggcgagcaac tcaacagaag
     1921 aacgtttatt tagtgtactt aaattaaata ttcttaaaca ctgaagagag aacacatact
     1981 atggctgaga ttaaagctaa actgtgttct ctttttgttt gagaaaggac gttacaatcg
     2041 atgttgagcg aaatttattc agctattctt aaaaacggct tacgtacata caaatatcga
     2101 aacagcacca tgaaacctgc aggtcatatt gattccagta ggagtggtgc tatctttgcg
     2161 taccgtagca agcaattaat gaatgttggg cgtggtatgg tcattacttc ggaagaagct
     2221 attttagaga acgaaaagaa actaacgcat tggacaccta atacctatcg ctttggtact
     2281 tatgcggatg attatcgaac agtggttaaa ggtcattctg agaaaaactt aagtcaaatc
     2341 aacacatttg tagttgatat cgattcaaaa gagaaccatc aaggcgaaat tattttagcc
     2401 tgtttagatc aagtgggcta catgccaacg ctaattttag aatcagatca tggttatcaa
     2461 gtttattttg tactaaagac accaggctat gtcacaaaga aaagcaattt caaggtgatc
     2521 gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt tggctgaaaa attgtccgga
     2581 gtggatctag gatgtaacca cttcggaatt gctcgattcc cacaagaaaa taatattgtt
     2641 tttaaagaaa tgaactatca gtattctttt gctgactgga ttcaatggtc gatgaagctt
     2701 gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac taattgtctt tcctgagaaa
     2761 aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt tacttcataa agcaaacatc
     2821 gtcggcggag aaggacgtct tggtcgaaat aatgtgattt tcacgctttc tttagcttat
     2881 tactcttcag gttatgcatt ggatacttgc gaatacaata tgttcacatt caacgaacgg
     2941 ttacaggaac cgctatctga agcagaatta gtaaagattg tacgtagtgc ttattctggt
     3001 aattatcaag cagcaagcag agaattcatt attgaattgt gccagcaatg ggtttcacca
     3061 accttaaaaa ataatgatct gttcattcaa cgacgtggct ggtggaaatt taaaaagcca
     3121 cgagatcaaa gaaaatatag tcataaaaat gaatggaaag aagatctatt ggcttatcta
     3181 agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt caaaaaaaga attggcttca
     3241 caactaacga tgcccaagcg aacacttgat aagatcttaa atgagttacg agaggagaat
     3301 aaagtcttct acaaggtaac acgaggaaga aatggcggaa tgatcattgc ttcagtcaaa
     3361 acgttgttcg cgagggttat tcaactaagt aaagaaaaga gactagatta ttcaacaagg
     3421 atcacagagg tatttggact caacgagagc ttagtagaag agacatttag acaattatca
     3481 acccccttga accctcttaa agaagtgaat ctttttgaag tagatacagg ttgaaaaata
     3541 aaaggtgcaa attgccttat acaatatatc tatgatactt ctgtaggtgt tgctcccttt
     3601 gcctagctac gtgtaggtgg tatacttata actgcgtatg cagtcttatg tatcatggag
     3661 gaagttgtat gtcgttaaaa aattatgtct attcacatcc tgttaatgtg tttatcattg
     3721 gtcgtttggg attatctgtg gaagtgttct gtgagcttta tggttttaag caggggactt
     3781 tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt gccgattgag tttcttcatg
     3841 ggttgtcctt agcttctggg gagactatgg atcaggtata tgattgtttg tgtgtgttag
     3901 agcaggagta tattgagtat aaaatagcta acgagttaag aaaaaggaag aagtatatcc
     3961 agtagtagtt tttatttact cttaattgtc aaattaagtt agacaaatca tcaatactta
     4021 cgtaactcaa aaatacttcc aaaggtgttc gataattcag tgattttcta ggaatattat
     4081 ttcgtttcga tgcgacagat tgaataaaag attcatctac tgaattgaaa tccatagatt
     4141 tcaataaacc atctctacgt aacaatccgt tagaattctc gtttaggcct ctttgagacg
     4201 gcgttcctgg atcagcgaaa taaatggcaa tatcattggc attactgatc tgtttccaat
     4261 ttgaaaattc ctttccacaa tcaaaagtga tggatttgaa tagatttttc ggtacagatt
     4321 caaaccaatg atttaatttg ttttcaatat caatcgcttg tctaccacac ggtttcaatg
     4381 tgatgataac ttttgataat cgctcaacta aggtaattac agcactttta tgtttcaaac
     4441 ctacgatagt gtcaccttca aggtgaccaa actcatttga gaattggcta taatcttttt
     4501 cacgttcatg aatagagcga cggaaagctt gttttccacg tctttcttga tgtccatttg
     4561 gtttacgctt gcctttcatc ggtaagtcag aaggattaaa tagccccttt ttgaacatac
     4621 gataaatggt acgagcagaa caacgaatag gaaacgctgc acgaccaaca atcacatcgg
     4681 gtgtccatcc ttgaacaacc tttctttgaa tatattctga ttgttcctca ggtaaaacaa
     4741 gcgggcgtct accacagttt gatttgtttt tcttatactg ttgatagtac tctaaggctg
     4801 attttccttg cttgaagaac aggtatactt tatggatcgt ttgtcttgaa cgattcaaga
     4861 aatgcgcagt tttcgcaaca gattgattta ttttgaaata agactctatc attacaagtt
     4921 catccgttgt aagatgggta taggtcattt gtactcactc cttataattc tttggacgga
     4981 actattttga gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc
     5041 gtacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     5101 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     5161 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     5221 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     5281 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     5341 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     5401 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     5461 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     5521 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     5581 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     5641 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     5701 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     5761 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     5821 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     5881 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     5941 tggaacagta gtgatttaga ac
//
LOCUS       DAPBAB010000111         5904 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPBAB010000111 DAPBAB010000000
VERSION     DAPBAB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.119"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(361..1479)
                     /locus_tag="RD444_002727"
     CDS             complement(361..1479)
                     /locus_tag="RD444_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550040.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1950..3257
                     /locus_tag="RD444_002728"
     CDS             1950..3257
                     /locus_tag="RD444_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDT7550041.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3376..4575
                     /locus_tag="RD444_002729"
     CDS             3376..4575
                     /locus_tag="RD444_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDT7550042.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4602..5870)
                     /locus_tag="RD444_002730"
     CDS             complement(4602..5870)
                     /locus_tag="RD444_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDT7550043.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1813 a         1252 c         1058 g         1781 t
ORIGIN      
        1 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa
       61 taatttttgt caatctcttc ttatttcttg aagctgacca cttctgtcac aacctcttcc
      121 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa
      181 gagctatttt tgcttgctgt tcttattact tgatgcagaa cagctttttc acaacctctc
      241 atacacggtg tttaatcagc tgaccttcgg tattagctca aaaaacggaa aaatatgtga
      301 aactattttt ccgtttgttt ctctaatact caggtcaaaa cactgatttc acaacctctt
      361 ttaataacga agaatcgctg tcagactctt ttcatctggc gcatcttttt gatccaaaat
      421 aaacactttc ccgccattaa tgatcacatc atctgctaat gcattcaaca cttgacgccg
      481 atcatattct gtttcaggat cttcaccgaa accatcagct aagtttgacg tagcgataaa
      541 taaatgtgac acttttcctt ctttcgcagc tggaacgata tcaactaact gatcgatgaa
      601 tttcttatct aagagtttat tgtattcttt tgattcttga gctgcgagtt cacttgacag
      661 cttcttcgcc ccttcttcga tatctctaaa ggatagctgc gcaggagaag cagaaatcgc
      721 aacgtctgca caataatagg gatttttagc aatttttttg aacatcgtct gattttctgg
      781 taatgcaaat aaataaatcg gccattgttc aggattttcg aaagaatcct tcaagtacgt
      841 atcaacagct tggtaatagt tgacccaatc gatctctact tcttcatctt ttggatttac
      901 gccatggaaa gttactccat ctcgggctcc gcctcctcca ttagaagaga agttcaaacc
      961 gccacctgtc agttcagatc ccaatgcagt aacgacatca accggtgccc cttctggtag
     1021 agatacttct tttacttgtt tgttgctgac ctcatacaat ttcatggagt cgcggttcaa
     1081 tcccaataaa taataagaat agttgaactg cccatttttg ataatagcca gtaaataagg
     1141 aagatcactt acatagtatt gatcgtctac cgggatactc aaacgatgga tataggtctg
     1201 gtcagcagtt ataatgaccg ctacactcgt agttgcacta cgccaaaacg aaggatctcc
     1261 taacaaattt tcaatttttt cttggaacgg ggaccagtca tgttcggaat attttttctc
     1321 aaaacgtttc tttgcttctt tcgcaaaatt tttgaatttg atctgatctt tttccacatc
     1381 ttgatgggcg acatgcgtat tcagcatgat cgtgacgaat ggtcctttag cgccttctga
     1441 agtcaattgt gtcaataatt ctttatcctt ttttgccata aacgtacccc tcctaaaaaa
     1501 atcattcctt tgtttctatc taaagtctac cacaacacta tttttccata caataaaacg
     1561 tatctaaaaa aataaaacgg tttctacggt tttaaattta gctatttttt taagttatct
     1621 tcataatttc ctcaattatt tagccccagt cttcaactat tcttcatgaa atgctgataa
     1681 actaagaaaa agaataaaag aggtggatct tatgcatttg attatgattt tcagtttatt
     1741 aagtattatc agtttatttg ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc
     1801 tattcctgta aaagtacgta aaaacgaccg ctaaacttcc ataacgcgcg ttcttatcct
     1861 aaagtttttt cagaaacatt gaaactctct cccctctctg ataaaatttt aatttgtagc
     1921 gaaacaaatt aattacaagg agatcggata tgtccataaa aaaaacacgt ctagtcctat
     1981 tattggctag tctcattctt tgccttgctt cttttctacc agctgttgct gtgcaagctg
     2041 aagatacgtt caaagtaaat gcaaaagcag cttttgctgt agatgcgcaa agcggtaaga
     2101 ttctttatga tcaagatgga gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg
     2161 gtctttacat cgtccttgat caagtaaaag aaggaaaact gtcatggggt gataaagttt
     2221 cgatttcaga ttatgcagaa aatttgagta tcaccccaga tttatcgaat gttcctttgc
     2281 ataaagaaaa cacctacact gtcaaagaat tattcgactc tgcaatcatc cagtcagcca
     2341 atgcttcaat ggtggccttg gcagaaaaaa tctctggcag cgaagctaaa ttcactgaac
     2401 gaatgaaaga acaattgaaa gattggggaa tcaaagatgc aacaatcgtt aatgcatcag
     2461 gattgaacaa ctcttatctt ggcgaaaacc gaccagaagg cactggagaa aacgacgaaa
     2521 atcaaatgtc tgctcaagat gtggccatcg ttgcccgcca tttgatcttg gatttcccag
     2581 aaatactgga tgtatcaagt actacgaccc aaatgtttgg cgaaaatacg caatcaccag
     2641 tggaaatggt caactggaac tggatgctcc caggattcgt taattacaag gaaggcgtgg
     2701 acggattaaa aactggtacg actgagcttg caggtgcttg tttcgttggt acgatcacta
     2761 aaaacgatca acggatcatc actgtcgtat tgaatgcgga cggccatgcg gaaaacccga
     2821 gtgcccgctt caatgaaaca gaccgtttga tggactactg ctatgacaat tggtcagaaa
     2881 aagagctcgg aaaagctaac gcaagtattc ctagtctaaa aaccattgat gtaaaagacg
     2941 gaaaagaatc aacagttcct gttgtgctta aaagccctgt aaaagtatgg gtacgaaacg
     3001 atatggatac tggcaaatta acgatcacgc caacacttga caagaagcaa gtcactgaca
     3061 atgagatcca agcacctatg gataaaggta caaaaatcgg taccgctacg atcaccttgg
     3121 cagacgataa attaggctat ttagaagaca acaatgcacc atcaacagag atcattacgg
     3181 ataaaacagt ggaaaaagcg aatatattcg tgattggctg gcgacgcgtc gcagaatttt
     3241 tcggcaactt gttttaattg aacaataaga aaaacatctt tccatcggaa agatgttttt
     3301 ttgctttttc tagtttaaat aacagctttt ttcatctatt ttcgttatac tgtataagaa
     3361 aaaggagttg aatttatgaa tctaaccaga gatctgatct ttttagttct ctatcttatt
     3421 ggggtattct ctatcgttta ttggcgaaaa cagaagaagg atccttcctt gttcttttac
     3481 ggcagtacgt ggacatttac ggtaatctta acttattttg tcgtgttgga aattttttat
     3541 tcgacctttt attttttgat tccattgttg ttctttagta tctttgcttt ttcttatttc
     3601 acagaaaaac gaaggctact gaatggtctt ttattcaatg ttttactcat cagtttcggc
     3661 atctacttgt ttgtcttgct gtatgaaacg caaaatattt ttcttggcgg actgatcgcc
     3721 ttgatcacga ttccattgct acttgtcctt ttatttggta tttatggatt gattgttttt
     3781 cttttttgga acggggtcac agttttacgt agagaatcgc attcattagc taatctttta
     3841 acgttgatac tagccatttt tcttacactg tttcttgttt ttgacttttt cttgttaaaa
     3901 tatttgccgc aatggataaa tgctttgttt ttctgcgttc ccctgatctt gatctatcta
     3961 tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa ccggcctcga
     4021 tacaatcaag acttcatcgt tgtgttaggc gctggattga tcaatggaga aacagtttct
     4081 cctcttctag ccaagcgaat caataaggca attgcttttt atcgggctca aagccgtgct
     4141 acattaaatc cgcccatctt attgatgtct ggcgggcaag gagcagacga aaaagtacca
     4201 gaggccattg cgatgaaaca atatgccatg gaacaaggaa tccctgaaag agacattctt
     4261 gtcgaaacca attcgacaac cactttggaa aacatgctct attcaaagga aatcatggat
     4321 cagcaaatga aagggccata ccgcgcgatc tttagttcca acaattacca catttttaga
     4381 gccggtcttt atgcgcggca agccaaattg aaagcaaatg gtattggtgc aaaaacagct
     4441 ttctactact tgcctaatgc gtttttaaga gaatacctcg ccatcttgat gatgaacaaa
     4501 aaaagacata tgatcatttg cggattgctt ttcgcaggct ccgcattctt atcgctgatt
     4561 actttacttt tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct
     4621 ttcattctct taactattgg caaaaagagt atcccttttt tgaccgctac ttgctgagca
     4681 gaataaatac cgctgttgcc tgaacacata aagctgatca agcagatcat aaagaagtat
     4741 actgccgctt gtgaaccgaa caattcgatc cccatcacaa agcaggcaat cggtgtattt
     4801 gtcgcaccag aaaatactcc gataaagccc aatcctgcaa gaaaaggaac agaaacatgg
     4861 agcaaaggcg ctagtgctgc accaagcgtt gcaccaattt caaacaatgg tgtcacttct
     4921 cccccttgat atcccgcgcc taaagacaaa actgtgaaga ataacttccc aataaaatca
     4981 tagacatgtg cctgtccatt aaatgattct tgaagtaacg gaagactcaa tccgagataa
     5041 cgttgtgttt gcaaaatcaa tgctgctgcc acaactacag ctccacctat cgtatttcga
     5101 caaacaggat gaggaatcca ttgtgcatat cgcgctttta aaaagacgat cgaacggctg
     5161 aatacccaac caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac
     5221 atactccatt caggaatcac acccatttgg taatgtagat gagaaacacc atagctttct
     5281 gtcatgaaat ttgcaaacag tgcagcaaag aagctaggga agatcgcttc tgttcggact
     5341 ttcccgattg ccaacacttc taatccaaat aatgttccag ctaaaggtgt gccaaataca
     5401 gaactaaaac ccgcactgat tccactgata atcaagattt cccgttctga tttatccaat
     5461 cggaacaaac gtgccgtatt atctgctaac gctcccccca tttggactgc tgttccttca
     5521 cggccaacag atcccccaaa taaatgggta gttatggttc caaacagtgt aagtggaata
     5581 agccgcaacg ggattttttc ttctccacca tttccttgat cgatcactaa gttgtttcca
     5641 cgactagaaa gtccgccgtg atacgcatag agataagcaa aaagcgcacc actaactggt
     5701 agtagacaaa ggagccaagg atgtgatagt cggatatctg tcacaactgt caggctattt
     5761 aagaaaaaag cagacaagct ccccatgaat ataccgagaa tcgctgtgat caccagccac
     5821 ttcagcatat acactaaaat gcgtactgga ctcggtaatt gtttttccat aaaaaattcc
     5881 tcctcaataa aaaaacgcct acta
//
LOCUS       DAPBAB010000112         5897 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPBAB010000112 DAPBAB010000000
VERSION     DAPBAB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.120"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(976..1149)
                     /locus_tag="RD444_002731"
     CDS             complement(976..1149)
                     /locus_tag="RD444_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550044.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1152..1550)
                     /locus_tag="RD444_002732"
     CDS             complement(1152..1550)
                     /locus_tag="RD444_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550045.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1766..2821
                     /locus_tag="RD444_002733"
     CDS             1766..2821
                     /locus_tag="RD444_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDT7550046.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2841..3194
                     /locus_tag="RD444_002734"
     CDS             2841..3194
                     /locus_tag="RD444_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550047.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3376..3672)
                     /locus_tag="RD444_002735"
     CDS             complement(3376..3672)
                     /locus_tag="RD444_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550048.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3676..4494)
                     /locus_tag="RD444_002736"
     CDS             complement(3676..4494)
                     /locus_tag="RD444_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7550049.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4720..4941
                     /locus_tag="RD444_002737"
     CDS             4720..4941
                     /locus_tag="RD444_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDT7550050.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4943..5209
                     /locus_tag="RD444_002738"
     CDS             4943..5209
                     /locus_tag="RD444_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7550051.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5322..5861
                     /locus_tag="RD444_002739"
     CDS             5322..5861
                     /locus_tag="RD444_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550052.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT         2065 a          982 c          874 g         1976 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc atttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaatcattt attatgctct tttataagga tattaattga
      481 tctaatttga aaaaaatcaa aatttaaatg ttttaatatt acaaataaaa tcatgttatt
      541 gtaaaatggt ataaaaaagc taaatattct atttttgtta ttttattagc aggaaaattt
      601 aaatagtttt acaagaatcc gtagaaatat attggttttg ccttcagatt aatactggat
      661 catattaatc tgaatataat atatgaacta ataaatagct taacctcgct attttgtttt
      721 ttctgatcac tatctataca gcattagtaa atagaatttt aaaaataaaa aaattaagac
      781 aaaaaagtat caacataaac atatacatat acacgtttat gttgattcac ttaatttgtc
      841 ctttaaaaag cacttatttt aatataatgt tattttataa tcttattaca agctgattgt
      901 taggcgttgt cctttcatta catttattaa cggttatttt attgtttaat ttattaatta
      961 tttttgatct aaaacttagc ttaacaaata aataattaac caaccgccag ataaaaatgc
     1021 gatcattgct aatacataag caaagataaa aatatgttcg atcagcttaa accctgatgc
     1081 gattaagcag accaagtaaa atagaaattc tcttacagat aatggtaatt ttctaactag
     1141 catactcata attaacaccc caaagtaata ttgtattttg aacattttac atccaataca
     1201 gcatcaatca accattgcca atcaaaaata caaggcttca ttgaagtatg tttttcagat
     1261 gaaaagatat aatataattt cttcggaata atattttcag ttgttgggat caagctaaat
     1321 ctaataccgt ctaactcata gctaccagct ttctctccat taaaaatttc cttagcactt
     1381 tcttctaaag aatcaaaata tttttcgaca gcaatctttg aagcttttac tattgaagca
     1441 tttccctttt cgataaaatc tgcttttgtt ttaccgacaa taagtaattc ttgatcagta
     1501 aaatcttcta aaataatggt agtgtaattc ttatttcttc tcaatcgcat caggatcact
     1561 ccttaatata atttatgttt ttataatatc ataataagat aaatttgcaa gcatttattt
     1621 acaccttttt atttacactt tattataatt aattagactt ttatgattgc atttttttct
     1681 tataaacagt tgcaactaat agtaaatagg tgtaaactat tttcgtaccg aaataccata
     1741 aacacaaaca gaaagaggtc ataccatgaa agaaccatta ttgttttccg ttgacttagg
     1801 gaataaatca gtaaaagaaa tgagtacaca cgtttctcca ctctcacttc cctctcgtat
     1861 tttgaacaaa aatatcatta attcaggacg tttttctaat ataggttcct cattaaaacg
     1921 atcactaaac gtttctgaat atagcctagt aaacactact gaaagctatt actttgggaa
     1981 ggatatacac gagctaggaa aagatgacta cgtgatagaa tcactaggtt ttgggatcag
     2041 cagatacaag caggaaacct accaaaatat cctatctttc tctttagcta acctagcacg
     2101 tttagaagat caatctgatg acgttttaac cgctaatcta gttttaggtc tgccttcatc
     2161 cgactatacc gaaacaatcg tcaaatacgt gatgcaacaa gccacacgac aacattcagt
     2221 tgaagttgac ggtaaagctt ataatataaa cgtagatacc gtttgggtac tgccacagcc
     2281 gattggaaca ctatataacc tcctacttgc tgatgatggt agtgtacaag atgaaacatt
     2341 gatcagtcag aagatcggtt ttgttgatat tggtggtggc acgaaacttt ttgatgttgt
     2401 ccaaaatttt gaaattgacg aaaacaaccg tgaacaaaag gataccggta tttttacttt
     2461 atataatcgt atcgtaaata tgcttgacct tccgatcagt cctaatgctt tccaacttga
     2521 aaagattatc cgagaaggca aagaaaaagg cgtatatcta tttaaaccaa gcggaaatga
     2581 aagttttgat atttcagaag tagttgaaag agaaataaaa acatacacca atatggttgt
     2641 tgcagaagtt ttagctacat tcaaaaactt cacaacctta gataaagttg tctttactgg
     2701 tggtggggct tctattatcg acaaacaact tgtaaaagat aaaattccgc ataccttgtt
     2761 tgttgaagac agtgaatttt ctaatgtaaa aggcttttac aagtttggtt taactcaata
     2821 aaacagcgaa ggagatcaac atgaataagg gaaaatcaga gcgttatgta gttataatca
     2881 atagagaaaa ggttagcgag ttacctttag cagagtttct tgaatctgaa tatgaaacac
     2941 taggcgttaa atactctcag acgatcaaaa aagctttgaa tctatatcgg cgtgtaaaga
     3001 acaatgaagt tacaattatt gaagcaggca acgatagaaa cgatagcaaa gtagctgaag
     3061 taaagaataa accaactgat cataacccta ttcaacaagc gcatccttca acggttacat
     3121 ccgatcattc agcaaagaaa gaaaagccaa tgctaaaagg gttcaatgca ccaagtaaca
     3181 aagacttact ttaaaaataa aaaggcgagg ttacaatacc tagccttttt attatgtaca
     3241 aatacaaaca taaacatata cgtatacatg tatataaaca tgtatacgta tatatccata
     3301 aacacgtata cgaaatttag ttaagctata cctaaccgct tattccaata aaaaaagtga
     3361 tcagctttaa actgatcact caccaaaaac ttcatcatac aagttatcta tcatttcacc
     3421 aagtaaagct gaatctgatc gaattcctaa cttttcgctg attttagata gtttctttct
     3481 ttgtgatggt tgaagcgtga attgataatt tttcttcttt tcagaactat cactttttag
     3541 tttagctacc aaagaacttg ttgtttcttt aggtgtttgt ttaggttctt caactgcttc
     3601 aaccacttca ggaacttctt ctacttcagc ttcagcgaat tttttaaatc tattttcttt
     3661 cacttcaatc actccttatt cttgtgccgt atcaattatt ttgttgtata gcgtaaacgt
     3721 gctttcaaag aaatccttat tcttacgata gatattctta tccttagcga actcagcaat
     3781 attcttacct tctaagctag ttgcattaag caattccttc tcttgtacat atccaagata
     3841 attagataac gttggtacca attctaggaa ctcacgactt gaattagtat tatgtttgat
     3901 cctgctaccg atcgtagcgt atttagtttc catcagtgtt tcaccagtct taatatcaat
     3961 caactgtttt gctagtgcat caatatcttt accaagctta agcaaagctt caaaaccatc
     4021 tttagatgga tcagaaatgc ctacgacaag atcagataca gcccaagcat tttgagctag
     4081 tagacctgca tcattatgag tatcaatcag aattagatca tacttctctt taagctgttc
     4141 atagttaaca acgatccatc taaacagtag ggttctgttg ttaggcatat cacgcatttg
     4201 catctcaact tgttttaaat cttcacctgc tactaaaata tccatgttct catttatagc
     4261 taaaggttca gctgatccat gaagatataa atgattgatt gtattctctc ttttcgcttt
     4321 actgaaagca tcaggaaatc gttttgatag gttttgcgac ttatcttcat caatatacag
     4381 caccttcata cctaaatttt cgacagcata tccacctaga ttgtagattg ttgttgtttt
     4441 acctgttcca cctttttcaa tgtttccagt aatgattgac aacttctttt ccatgattaa
     4501 acctccctta ttaatctttt gtaactccat tatacactat aaacacgttt atgtatatat
     4561 ttatatacat aaacgtgttt atatttatat ttgcttttat attctacaac ataaacatat
     4621 atatgtatat gtttatgttt atgtatatat tcaaatatat aactattgca tttgtcatac
     4681 aatcgtagta caatgtaatt gaaaaggagt tgataaacta tgccagtaat aagtattcgt
     4741 gtatctaaag aagaaaaaga ttttcttgaa aaagttgctg aattcaacgg agataacatt
     4801 tctgaattta tgcgtagtaa ttccctgcaa acagcagaaa cgttagtaga ctttgccacc
     4861 tacaagcaac taatgaaaga gcatgaggaa aacgatcaaa gtatttccca cgaagatatg
     4921 ctaaaagagt taggcttata agatgactta ccaagtaaga tatgaacgtg aagctcaaaa
     4981 agcgctaaaa aaaatggata agtttcaggc aaaggttatc ctaaactgga tcgagaaaaa
     5041 tcttgtaggc actgatgatc caagaaagca tggtaaaggt ctaacagcta ataaatcagg
     5101 ctactggcga tatagggtag gtgcataccg tataatagct gatattagcg agcatgaagt
     5161 gacaattctt atattgtcta ttggtcacag aagtgaagtt tataagtaac agacaagctg
     5221 ataaggcttg tctgtttttt tgttaaccca acaaaatcac tgtttacgat cccactttac
     5281 ctaaaaaaga acaatatcct cttgatatta tacacaccag tgtgtataat ataagtgtaa
     5341 acaagctaat taactttcaa aaggagcgat tttttatggg atggtatggt gattattcat
     5401 cagcaaatga agtgatcgaa gaaaagaaag ggtacgccaa aaatggtttg gaaatcgtag
     5461 gtaccaaagt aacgaagacc tatggaatta tccttttcaa agatgcagaa ggaacctacg
     5521 atattgacag cttcatcttt cgagatgaaa tgtacaaacc aatctacttc ttaaatgaca
     5581 gtcctgctta ctttaagaaa attccgaaaa aatggttgaa attctttgtc ggtgatcaag
     5641 aaaaaatggt taagctacaa ctagatagaa ttaaagaaga aagcaataaa ccaaaacttg
     5701 atgacttatt agttgcaggt aaaacttact caatctggga tggatcgtat gtcgctgtct
     5761 atcagtataa actcaaacgt agctttattt ttcgactaca agatggatca ctcactcggt
     5821 tcactcgact atcagcagga gatgttaagg aagtaaccta aaacttaaat acataaacat
     5881 aaacgtgtat acgttta
//
LOCUS       DAPBAB010000113         5836 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPBAB010000113 DAPBAB010000000
VERSION     DAPBAB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.121"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(336..1112)
                     /locus_tag="RD444_002740"
     CDS             complement(336..1112)
                     /locus_tag="RD444_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDT7550053.1"
                     /translation="MIGFIYFIVIVLANTVGAVSGMGGGVLIKPIFDFIGAHSVAAIS
                     FYSTVAVFTMSLVSTARQLASGRKIHWQIVLWVSGGAVLGGILGNVAFDHLLLLFQNE
                     DEVQMIQIFLTVVTLLFAFFYTKYDWPGFQLKNNFWYCFCGLVLGFLASLLGIGGGPI
                     NVSLLMLMFALPIKEATVYSICTIFFSQLAKIVTIAGTTGFGLYDLKILWFVIPAAIV
                     GGLLGAKVSTVLSPQKVTLVFQTVIFLVLIINLYNGYQLL"
     gene            complement(1109..2221)
                     /locus_tag="RD444_002741"
     CDS             complement(1109..2221)
                     /locus_tag="RD444_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HDT7550054.1"
                     /translation="MKHISVLIKPASSLCNLRCSYCFYANVSSLREVRSFGKMKEEVT
                     EKMIENIYADLEDGDQLTLAFQGGEPTMAGLNYFRTVIRLVAQQQKQVQVHYAIQTNG
                     TMINEKWCAFLKEHDFLVGLSIDGHPLYHDLHRVDPKGRGTFQRVLQTKQLFDYYEID
                     YNVLCVLTNPLAKEAKKVFRFLKEQQIDFVQFIPCLDDLDAKKRNSYALTPKRFAGFY
                     HQLLQLWLQELAEGHYISIKLFDDLLNLLIKRQVTACGILGNCQVQYVIEADGSVYPC
                     DFYVLDEYRMGYIQEQTLRQLFEQDIAKTFLCGRPEMPKKCLSCPFQKMCRGGCKRMK
                     DAIYVDNTGYCGYQQLLQEFVPQINRILGLLQGVSV"
     gene            complement(2302..3006)
                     /locus_tag="RD444_002742"
     CDS             complement(2302..3006)
                     /locus_tag="RD444_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7550055.1"
                     /translation="MIKPKYLEIKQNLKQQIVTGQFHNGDRFYSESEIIKKYNVSSIT
                     AIRALKELVAEGYIVRYQGIGTFVSRARKEKIVKFSDIEIFSFDNDKTHVLSIKRENK
                     KQILDTLKLSTDSYYYQIERIREANEIPYIYHKSYLPEQYINSNYPEINYYNSIYKRL
                     KSDYNIDLNEENFRETNEILFPAPNSVCKQLKINESEPVVFQTKLSCLSENNKPIEYV
                     ESYKKWNFYKIQLLSN"
     gene            3126..4904
                     /locus_tag="RD444_002743"
     CDS             3126..4904
                     /locus_tag="RD444_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDT7550056.1"
                     /translation="MTTFEIKEEFLLKGKTFKILSGAIHYFRIPPCDWEHSLYNLKAL
                     GFNTVETYVPWNLHEPQKGEFHFEGILDLERFLTIAQDLGLYAIVRPSPYICAEWEFG
                     GFPSWLLREPIHIRRNEIAYLEHVADYYDVLMKRIVPHQLNNGGNILMIQIENEYGSF
                     GEEKEYLRAIRDLMIKRGVTVPFFTSDGPWRATLRAGSMIEDDILVTGNFGSKAKDNF
                     NSMKQFFKEYDKNWPLMCMEFWDGWFNRWKEPIIQRDPQELAEAVKEVLEQGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYALRKMIHDNYPEIK
                     QLDPVIKPTIEKKKISLTNKVSLFATLDTISQPVQSKYPKTMEELGQNTGYLLYRTMI
                     ERDAEEERLRVIDGRDRSQLFLNQELQATQYQTEIGEDIQVSMTEEINQVDILIENMG
                     RVNYGHKLFAETQKKGIRTGVMADLHFITNWQQYSLPLENCKTVDYSKGWQPQHPSFY
                     RYEFLLEDIGDCFIDLRKFGKGIVFVNQTNIGRFWEVGPTLSLYISSGFLKVGKNEIV
                     IFETEGTYEPEINLLKAPLYKEMKEG"
     gene            4908..5387
                     /locus_tag="RD444_002744"
     CDS             4908..5387
                     /locus_tag="RD444_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDT7550057.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVAQNDIEK
                     SGLKLATPAGVKLSILPIEKAAQNILAGKYDSQRLLIIARKPDRLLKLVELGVPIKEI
                     NVGNMSQTSETRSITKSINVVDQDVETFNQLNEKGVHLIAQMVPSDKADDFMSLLNK"
     gene            5410..5817
                     /locus_tag="RD444_002745"
     CDS             5410..5817
                     /locus_tag="RD444_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7550058.1"
                     /translation="MAGHLVLISHGRFCEELKRSVDMIMGVQKDIYTVGLLPEESAKD
                     FRVNFEKTINGLKKVTVLADLMGGTPCNVVSQLILEGKEIELYAGMNMPMVIGFVNNQ
                     FLGEDSSLVNKGRENIIHVNEYLEVNPIDLEDE"
BASE COUNT         1967 a          945 c         1143 g         1781 t
ORIGIN      
        1 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
       61 gccagaatgt ggttcattct gacccagatt tgattttgca caactcatca gtccttattg
      121 acaaagagcc tattttgtta gtagatgtct ggaaacggta ctttgcacat ggtgaacagg
      181 cagtgctatt agaaaaatca tgcatattac taatgcaatt gagagtattt attctagttt
      241 cagaaaagtc acaaaaaaga gcgcttctta atgagaaagt acttctaaag ttactaatta
      301 atcgttttct ataagtggtt gataatactc gttttttata gtaattgata gccgttataa
      361 agattgatga ttagcaccaa gaagatgact gtctggaaga ccagcgtcac tttttgtggt
      421 gagaggacag tgctgacttt tgcccctaac aaaccgccaa caatcgcggc tggaatcaca
      481 aaccacaaaa tctttaagtc atagaggcca aatccagtgg tcccagcaat cgtcacgatt
      541 ttcgccaatt gagagaaaaa aatcgtacaa atcgaataga ccgtggcttc tttgatgggt
      601 aaagcaaaca tcagcatcag caaggacaca ttgatcggtc caccgccaat gcccagcaga
      661 ctcgctagaa aaccaaggac taggccacag aagcaatacc agaagttatt tttcagttgg
      721 aatccgggcc agtcgtattt ggtataaaag aaagcaaaca gcagtgtcac aacggtcaaa
      781 aagatctgaa tcatttgtac ctcatcttca ttctgaaata aaagcagcag gtgatcaaaa
      841 gcgacattgc ccaagatgcc tcctaagacg gcgccgcctg aaacccaaag gacgatttgc
      901 caatggattt tacgtccact agccaactgt cttgcggtcg aaaccagcga catggtaaac
      961 acggctacgg tagaataaaa cgagatggct gcaacagaat gggcaccgat aaaatcaaaa
     1021 atgggtttga tcagaacacc gccccccatg cctgaaacgg caccaaccgt attcgccaac
     1081 acgatcacta taaaataaat gaacccaatc acacacttac cccctgtaac aaacctaaaa
     1141 tccgattaat ctgcggtaca aattcctgca aaagttgctg ataaccacaa tagcctgtgt
     1201 tatctacata gatggcatct ttcatccgtt tgcagccgcc acgacacatt ttttgaaacg
     1261 ggcaactcag acatttcttt ggcatttcgg ggcgtccaca caaaaacgtt tttgcaatgt
     1321 cctgttcaaa taactgacgt aaagtctgtt cttgaatata ccccatgcga tactcatcca
     1381 gcacataaaa atcacaagga tacacactgc catctgcttc aatgacatat tgtacctgac
     1441 agtttcctaa aatcccgcag gcagtgactt gtcgcttgat caatagattc aaaagatcat
     1501 caaaaagctt gatacttata taatgcccct ccgctaattc ttgcagccat aactgcaaaa
     1561 gctggtggta aaatcctgca aaacgttttg gtgtgagagc ataactattt cttttttttg
     1621 catctagatc atctaaacaa ggaataaact ggacaaaatc aatttgttgc tcttttagaa
     1681 agcgaaagac tttcttagcc tcttttgcca acggattggt caagacacaa agtacattgt
     1741 agtcgatctc atagtagtca aacagctgtt ttgtttgcag cactcgctga aaagttcctc
     1801 ttcctttcgg atcgacccga tgcagatcat ggtagagcgg atggccatcg atcgagagac
     1861 cgaccaaaaa atcatgttct ttcaaaaaag cacaccattt ttcattgatc atggtcccat
     1921 tcgtttgaat cgcataatgg acctgtacct gcttttgttg ttgcgctact aaccggatca
     1981 ctgtacgaaa ataattgagg cccgccatcg ttggctctcc accttgaaac gctaatgtta
     2041 attgatcgcc gtcttctaag tctgcataaa tattttcgat cattttctct gtcacttctt
     2101 ctttcatttt gccaaacgag cggacttctc gcaaggaact gacattggca taaaagcagt
     2161 agctgcagcg aagattgcag agggaagatg cgggtttgat caatactgaa atatgtttca
     2221 tagtaagaac ctcatttttt aacatagttt aattatgaaa aattgctata acgaacaaag
     2281 taaaatcaaa ttcagtagta gttaattaga taggagttga atcttataga aattccattt
     2341 tttataactt tcaacatatt ctataggttt attattttca gataagcagg ataatttggt
     2401 ttgaaaaacg acaggctcag attcatttat ttttagttgt ttacatacac tattaggagc
     2461 aggaaataaa atttcgtttg tttccctaaa gttttcttcg tttaaatcaa tgttgtagtc
     2521 tgattttaaa cgtttgtaaa tagaattgta ataattaatt tctggataat tagaatttat
     2581 atattgttct ggcaaataag atttatgata gatataaggt atttcattag cttctctaat
     2641 tcgttcgatt tgataataat atgaatcagt tgataatttt aatgtatcta atatttgttt
     2701 tttattttcg cgtttaatcg aaagaacgtg tgttttatca ttgtcaaatg aaaatatttc
     2761 tatatcagaa aattttacta ttttttcttt tcttgcacgt gaaacgaaag ttcctattcc
     2821 ttgataacgt acgatatacc cctcggcaac taattctttt aacgcacgaa ttgctgttat
     2881 agaactaaca ttatattttt tgataatttc tgattctgaa tagaatcggt cgccattatg
     2941 aaattgaccg gttacaattt gttgttttag attctgtttg atttctagat atttcggttt
     3001 tattataagc tcccccttta aattactcgc atctatatta acatacttaa aaaaaaattg
     3061 ttgactttta atacaaaagc attaatatac taagtatatt aacacattga ggtgaaaagt
     3121 aacagatgac tacatttgaa ataaaggaag aatttttatt aaagggaaaa acatttaaaa
     3181 tactatctgg tgcaattcat tattttcgga ttcccccatg tgattgggaa cattctttgt
     3241 acaatttaaa agcactagga tttaatacag tagagacata tgttccttgg aatttacatg
     3301 agcctcaaaa aggagagttt cattttgaag ggatattgga tttagagaga tttttgacaa
     3361 tcgctcaaga cttaggatta tatgctattg tgcggccatc gccttatatt tgtgctgaat
     3421 gggaatttgg agggtttccg tcatggctac tccgtgaacc gattcatatc cgaaggaatg
     3481 aaatcgctta tttggagcat gtagcagatt attatgatgt tttgatgaaa aggattgttc
     3541 cccatcaact taataatggg ggaaatatat tgatgattca aattgaaaat gagtatggat
     3601 cttttgggga agaaaaggaa tatttgcgtg cgataagaga tttgatgatt aagcgaggag
     3661 tgacagttcc ttttttcact tcagatggac cgtggagggc tactttacga gcgggaagca
     3721 tgattgaaga tgatattttg gttactggta attttggttc taaagcaaaa gacaacttca
     3781 actctatgaa gcagtttttt aaagaatatg ataaaaattg gccactgatg tgtatggaat
     3841 tttgggatgg ttggtttaat cgttggaaag aaccaattat tcaacgagat ccacaggaac
     3901 tcgctgaagc ggtaaaagag gtattagaac aaggaagcat taatttatat atgtttcatg
     3961 gaggaacgaa ttttgggttt atgaatggtt gttcagcaag aggagtgatt gatttaccgc
     4021 aaattacttc ctatgattat ggtgcaccat tagatgagca ggggaatcca acagaaaaat
     4081 attatgcatt gcggaaaatg attcatgata attatccaga gattaagcag ctagatcctg
     4141 tcattaaacc cacgattgaa aagaaaaaga tttctttgac gaataaggta agtcttttcg
     4201 cgactttaga taccattagt caaccggttc aatcaaagta tccaaagaca atggaagagt
     4261 tgggacaaaa tacaggttat ctactttacc gaacaatgat agagagggat gcagaggaag
     4321 aacgattacg ggtaattgat ggcagagatc gcagccaatt atttttgaat caagaattac
     4381 aagctaccca atatcaaaca gaaataggtg aagatataca agttagtatg acagaagaaa
     4441 tcaatcaagt ggatattttg attgaaaata tgggacgtgt caattatgga cataaactct
     4501 ttgcggaaac gcaaaaaaaa ggaattagaa cgggagtaat ggcagactta cattttatta
     4561 caaattggca acagtattca ttgcctttgg aaaattgtaa aactgtggat tattcaaaag
     4621 gatggcagcc ccaacaccca agtttttatc gatatgagtt tcttttagag gatatcggtg
     4681 attgttttat tgatttaagg aaatttggta aaggaattgt ctttgttaat cagaccaata
     4741 taggtcgttt ttgggaagta ggtccaacgc tttcactgta catctctagc ggctttctaa
     4801 aagttggtaa gaatgaaatt gttatttttg agacagaagg tacctatgaa ccagaaatca
     4861 atttattaaa agcaccatta tataaagaaa tgaaagaggg atgaacaatg agtattattg
     4921 gagtaagaat tgatggacgt ttaattcacg gacaggtagc caatttatgg acaaccaaat
     4981 tgaatatttc tcgaattatg gttgtggatg atgaagtggc tcaaaatgat attgaaaaaa
     5041 gcggattgaa attggctacc ccagcagggg tgaaactaag tattttgccg attgaaaaag
     5101 cggcgcaaaa cattttagca ggaaaatatg attcacaacg tttactgatt attgcacgaa
     5161 aacctgatcg attattaaaa ttggtagaat tgggcgttcc aataaaagag attaatgttg
     5221 gaaatatgtc acaaacttct gagactcgat caattacgaa atcaattaat gttgtcgatc
     5281 aagatgtaga aacgttcaat caattaaatg aaaaaggtgt ccatttaatt gcgcaaatgg
     5341 ttccaagtga caaagcagat gactttatgt ctctgttaaa taaataataa tatacgagga
     5401 ggtgaaaaga tggcggggca tttagtttta attagtcatg gacgattttg tgaagagtta
     5461 aagagaagtg ttgatatgat catgggagta caaaaagata tttatactgt agggttatta
     5521 cctgaagagt cagcaaaaga ttttcgagta aatttcgaaa aaacaattaa tggtttgaaa
     5581 aaagttacag ttctggctga tctaatggga ggcactcctt gcaatgttgt ttctcaatta
     5641 attttagaag gaaaagaaat agaactatat gcaggaatga atatgccaat ggttataggc
     5701 tttgttaata atcaattttt aggagaagat agttctttgg taaataaagg aagagaaaat
     5761 attatacatg taaatgaata tttggaagta aatccaatag atttagaaga tgaatgaatg
     5821 tggaggaatt tatgaa
//
LOCUS       DAPBAB010000114         5690 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPBAB010000114 DAPBAB010000000
VERSION     DAPBAB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.123"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(489..1298)
                     /locus_tag="RD444_002746"
     CDS             complement(489..1298)
                     /locus_tag="RD444_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7550059.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1298..2044)
                     /locus_tag="RD444_002747"
     CDS             complement(1298..2044)
                     /locus_tag="RD444_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7550060.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2062..2529)
                     /locus_tag="RD444_002748"
     CDS             complement(2062..2529)
                     /locus_tag="RD444_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7550061.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2529..2939)
                     /locus_tag="RD444_002749"
     CDS             complement(2529..2939)
                     /locus_tag="RD444_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7550062.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2950..3963)
                     /locus_tag="RD444_002750"
     CDS             complement(2950..3963)
                     /locus_tag="RD444_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7550063.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4180..4914
                     /locus_tag="RD444_002751"
     CDS             4180..4914
                     /locus_tag="RD444_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7550064.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4898..5608
                     /locus_tag="RD444_002752"
     CDS             4898..5608
                     /locus_tag="RD444_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7550065.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1833 a         1195 c          943 g         1719 t
ORIGIN      
        1 aaagcgacaa aaaccacgag caggtccatt catcctcaaa cctcggtgat cttcgcggtt
       61 taacaccatc agaagaaatg cacaaaaata ataaggagag tctgagacat aaataaattc
      121 ccagactttt agtgagtcta aatccgaata aacggtgggt cgaaagcaac tctagctgcg
      181 cgtcgatcgc agcaatctct aggtgctatg ttcggataaa tggtggtcaa aagtcagatt
      241 ttcggccatt tcgtaacagc caaccagaca aaaagcgtct gttttgtctt ctactccaat
      301 tgctcaaatc ttagcgactt ttggcttggt cttactctaa acagtttgta agcctaaact
      361 ttatttgctg tttgtttcta agatcaaagc aaaactactt tttagttggt tacttttaca
      421 agttcttttt actgttgaat aaatgtaaat cttaagaaca aatatttact tctataattc
      481 caagtttctt aaaaaattcc gccaagtgta cctaaaaagc ataaaacaaa aattcctaga
      541 ataattgtga tcgttttatg ccctttggag taaagccaga cgcataaaaa ggtaattcct
      601 aatggcagca agcccggaaa aatttgatca aaaatacttt gtaatgaagt ttcagctcca
      661 ccaaccgtga attttagtga cgtatttaaa gtaaccatcg tgcctatcat cgcgccaatt
      721 accgttaaac ctaaaatcga agcaccatga gataatttgt caatcatatg gttgtcttct
      781 gtatcatcaa taaatttagt tcctaaaaca tagccatatt ttgtcgccaa ccatttaaaa
      841 ataaaatgaa tggcagtata caaagtaaaa taaaggatca tgcctaaaat attcccttgt
      901 tgagcaaaag aaatacctat gccagccgca ataacccgaa aagttcccca gaaaaatgaa
      961 tcgccaatac ctgataaagg tcccattaat cctgttttga ttttattaat tgcgtctgta
     1021 tcaaattcag gatttttagc attttcttct tccatggtga cggacaagct cataacaaaa
     1081 ggcgcaatat gcggtgtaat gttaaatgtt tccgtatggc gctgcaacgc tgtataataa
     1141 tcatcatcgt tcggatatat tttacgtaag gaacgttcaa taccattcat ttgtccatat
     1201 gccatcattt tatcgaagga ccaagaggat tgcataaaaa aggagcgcca aaatatactt
     1261 cttaattctt ttttcccaat aacactttct tttaccatta taaatcttcc tcctcttcaa
     1321 aagttagctt ttgaacagcc agtaacgcta agcaaagtcc gacgacagcc acaccgacaa
     1381 catccatttt cagatacatt acgccgacaa atcctaagaa aagataaggc atcatcgctt
     1441 tattcgccat cattttaatt aataacgcta gcccaacaca agaaagcaca ccggccgcaa
     1501 catcaaagct gtccaataca acctgaggaa tcaacttgat catcgcattg attgtagaca
     1561 caccaaaata agtagctaca aaaactaaaa tggccgatgg aaccccaacg attaaaggtt
     1621 gagccacata atgatatttt tgtgcttttt tgaaatcatg attgactaat gcctcatcca
     1681 ttttgttgtt aaaatacgta attggcattg cataggcgaa gttacgccac atctgaagaa
     1741 agatggacaa aggtaaagtc aacgcaacgg cagttcctac tccttgaccc gattgaatcg
     1801 caattgctgt acctaaaaca ctggaagcat aaacttcagg gggcgtcgca ctccctacca
     1861 taattgagcc catgaacatg gattccaagg tggcaccaat aataattcct gactctaaat
     1921 ctcccaaaat caaaccgaca aaaggcgctg taaatagcgg tctacccatc atcgatgcac
     1981 caaatgtgta ttcatcaata gacgccaaaa aagctaccag tgcgattaat attgcttgta
     2041 tcatattctt cccctcctct tttagtcatg caaaggattg atttttttgt ccgttggtac
     2101 agcctgacat tgaactgtta tcccgttttc agtcattttt ttcaccaagt ccagttcttg
     2161 ttcattcaag taaacggaac tagtaatcat tttccgattc tcgtttttct ttaaatttcc
     2221 taaattcact tctttaatct caggtacgtg ctgagtaatt tcgtaggcat cctccaccgt
     2281 cccgacaatt aaaaatactt ggtatttctt gattttttct ccatttaaaa cagagatggc
     2341 ctctgtcata tccttcacca caaattttgt ccctgtagga acagccattt ttagtgtttt
     2401 tttgcgcaga ttattggtag cgatttcatt acttgcaacg aaaatacaat ttgcacctaa
     2461 gttatttgtc caggcaaaag caacttgccc atgaacaaaa cgttcatcta ctcgtaataa
     2521 tttaatcatt aaaaatcatc ctcttctgtt ttctctagca actcgttcac caaaactatc
     2581 ccttcattcc cgctattcat agctttttgg attagttttt tcgtgtcctg ttcacctgat
     2641 aagcaaagct ctaaaagcag cggcagattt acaccgctga ttaaataaaa attgtcttta
     2701 ccgatttgtt cggagaaagc gttagacacg cttcctccca gtaaatctac cgtcacaatt
     2761 aattcgtcat caggcgctaa gtcggaaaat agctgctcta tttcctggtt aatggagtag
     2821 tcactatcta tatacgcagt tattgctaaa agtgattttt gaggaccaat aataaattct
     2881 aaagtatcct ttaagccttt agccatcatg gaatgcgtcg caattacaat tttcctcata
     2941 gctcttacct taatattcca cagtccgata ataacgacga atatctagtg aatgatcccg
     3001 caccttttca aaatgagcac tcactcgttc taaggccgtt gccataacca ttggcgctac
     3061 atactgacgc atagcgcttg gtacacctgc taattcgaaa tccgcagtgt caaaaaccgt
     3121 cacaacatcg ctatatttat ttacaaaacg ctctacgcga tcgactagcg gacgcgtttg
     3181 atcttcacct tttaataaca caaaactcat agatttatca gtcatttcaa tagtcccatg
     3241 gaaaaattcg gctgcatgga tcgacttggt ggcaatccat tgcatttctt ccagtacgca
     3301 catagcatat tcataggcag ccccataagt attcccggct ccaacaaaca tatgaaattc
     3361 cgtattttta tgtttttctg caaattcaag tgctttctga tcattttgtt gccgaacact
     3421 tagtaatact tccggcattt tttgtaatgt ttgaataaat tcatcatatt caggaaactc
     3481 accgttattt ttcatcaggc gtgcacccaa agcaaaaaac tgtaactgaa gttcttctac
     3541 taatgtatcg ttgtcagcat cattttcaat cgcataatcc acgatttccc cgagtggtgt
     3601 agcagcattt cctacaaacg caatcgtcgt tgcaccttga ctattggcat acttaacaac
     3661 atcaacggtt tcttgagtcg taccggttaa tgaagatgtg attaacactg tatctttatt
     3721 aatctttttc ggctgtgaac aaaccacctc tgcggctatt tcatgataat aatcaagctc
     3781 tgaatgagtg cttaacagat aagctagcgg gctgattgtg gcatatgttc caccagagcc
     3841 taccaaataa agatttttaa atcccttctc actgattgca tctgccacct gattgattgt
     3901 ctcgatattt tttactgcac catttaatac tgatagaaaa cgttcttcgc taaaacctaa
     3961 catagttaaa aacctccaag tcaataattt gaaaaatata tttgcaaatg ccggcacatt
     4021 aacaaaccac ttaacctttt ttatatacca cctgttaacg tttttaatgt accacttata
     4081 gaaaacgctg tcaatagttt ttaaccactt ttatttttat ttgtaccaaa atcctttttt
     4141 catgtataat aaacaaaaat agactctgga gtgtaacgta tgaatactga agaactaacg
     4201 aacaaattaa tacaagacat tgaacaagaa aaatataaat acgcgcaagc tattccttca
     4261 gaacgtgatt ttgttcaaat ttatgaaatg aatcgttcag ccgtaaaaaa agccattcaa
     4321 caattagtaa ataaagagta cctactaaaa atacatggca aaggaacttt tgttaaaaaa
     4381 aatatccatg aacatttgca aatcaaattt catggtttgt ctgaattgtt ggaacgggaa
     4441 gggattcagc ctagcagctt aattttagct tcagaaataa aaccggctgg acacattgtc
     4501 gggaaagctt tacagctgga accaactgaa gaagtatttc atttggttcg tttaagaagc
     4561 ggcgacggtg agccaatttc tattgaagac acttatattc cattaaatct catacctgat
     4621 cttcaagctt ttgatttcca aattttttca ttttattcca cattggctgc ttatcattat
     4681 caaattgaaa atatctatca gacaatttcc agctcaaaag tgagcaataa agaagcccgt
     4741 ttgttaaaaa ccagcttaaa tcgtccggta atcagccttg ctatcactgc ggttacgaca
     4801 aatgaacaag cggttgaata cactgaagta gtggttttgc ctgaattcgc cgccatctat
     4861 actaaaaatg ttcatcaagg aaaggagatg aaaatctatg cccaaattga ttaatctgtt
     4921 ggcttatgac ctgattgaca aaattattgc ttatattcat gaacatcatt taacccccgg
     4981 aacaaaactg ccttcggaac gggacctatc agaaatgttt aatgttaatc gaactacctt
     5041 aagaggcgcg ttacaaaaac tggtggacaa cggcacctta cgggcgcaaa ccaatagcgg
     5101 ctattattta actgcgccaa aattcgaaaa aaatgcccat catttcttta caccgataga
     5161 agattctctg cttagtaagc agcggctaca gctaaaggca acggctcttt caccgaaaaa
     5221 agcagcagct ctttgtccgg aactgaatga tttcaaacag cagcaaccac aagcttattt
     5281 agaatatatt gatgcagaag caattagctt gatgttatta atccctatgg aaacattaaa
     5341 gaaaagtaat ttctcggaca atcctttcca agattttaaa agtttggcat atacgcgttc
     5401 tcaagaaatt tctttaataa ctgacaatga cgttgcggcg gacttgctgg gagttgacaa
     5461 agaagaggtt ctgttatttg tacgagagga aataagagcc ggtaaggagt tggcggcgat
     5521 tgctttaggt tatactatta gtcgccgctg caacatacag gttaatgttg ctacgacctt
     5581 gcaatccggt tctgttgcaa agttttaaat ctactatcag ataaggtaga ataatagaaa
     5641 aagatagcag gaggaatgac gatgagtcat tttaaaggaa agcaatttca
//
LOCUS       DAPBAB010000115         5682 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPBAB010000115 DAPBAB010000000
VERSION     DAPBAB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.124"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            133..1287
                     /locus_tag="RD444_002753"
     CDS             133..1287
                     /locus_tag="RD444_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7550066.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="RD444_002754"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="RD444_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7550067.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="RD444_002755"
     CDS             3322..4584
                     /locus_tag="RD444_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDT7550068.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5682
                     /locus_tag="RD444_002756"
     CDS             4829..>5682
                     /locus_tag="RD444_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDT7550069.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1955 a          805 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa aa
//
LOCUS       DAPBAB010000116         5613 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPBAB010000116 DAPBAB010000000
VERSION     DAPBAB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.125"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            132..1337
                     /locus_tag="RD444_002757"
     CDS             132..1337
                     /locus_tag="RD444_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDT7550070.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTEHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            1353..1997
                     /locus_tag="RD444_002758"
     CDS             1353..1997
                     /locus_tag="RD444_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550071.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2008..2814
                     /locus_tag="RD444_002759"
     CDS             2008..2814
                     /locus_tag="RD444_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550072.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            2837..3067
                     /locus_tag="RD444_002760"
     CDS             2837..3067
                     /locus_tag="RD444_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550073.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYIAAKKYFTAERK"
     gene            3331..4203
                     /locus_tag="RD444_002761"
     CDS             3331..4203
                     /locus_tag="RD444_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550074.1"
                     /translation="MKSSDWMEHTWLPLKRNSKRKEFWILQLWPLGKLCSYLARFILD
                     LIIYSIVVFGTAYYLLNDPNRIAASDIFTVLMTLIFVMLFTIFTQIAMQFTRQKLTAG
                     KLFKSLFDLIIVNAILATLIKQIEQFGRTTKGLKEFLMTFVHFVNGYMYLFEVGIILF
                     LFGYISTKLIKRSIEKEIEIDLIPTLFGYEYYVDFGRYDELINTGIVHTSIYEATVKG
                     IRTTLKDGQESKAIDRRKEIYNFYEVEASLRYGIGKLSLDGRNIPQNKPYIGWKSDAL
                     EVSKKLSMEEDELQ"
     gene            4200..5597
                     /locus_tag="RD444_002762"
     CDS             4200..5597
                     /locus_tag="RD444_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HDT7550075.1"
                     /translation="MSSINLNHLFEDISNHFQQFSIEDNDELMGLFQIEKEYDYLTFI
                     CREGRIIDPKESKIISHLRRFKNYISKNKETISEIKFDGTTNLEYLVRVTYQRNRRLI
                     EDDQGVLICAGEEYDSLKFTQLKFESIGRSIYFESRPFYIRYNDFQHLFLQDEPNGLT
                     ILERRGKKQITLSYERPLTFMDGSCESIFQKINPFIKEMMKEKDSLPFTWAEIIEAKN
                     KYELLCKKYPSKKFTKKVNKYPLRHTYALIKLKPRVTRKQFRRIEAAIEQEDIDVFPS
                     ERFDFIRKNSREFVKSLLISYVQNCILPSFATIILSDLMDMSFYLNQKLEFNFKTERG
                     LLRQHNQIVEEYNLKQAKRLKTFKLKQHGKYRLLVKHLQNHPHFNLIKTNKELFMEGS
                     TMHHCVYNYLEKIQRGGSTIWKYERQKHRYTVEIEKRKYGNYEVVQCYGKYDSLPDKQ
                     ELDEIKKVVRAIPYK"
BASE COUNT         2067 a          825 c         1114 g         1607 t
ORIGIN      
        1 aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc
       61 tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa attgtttgat
      121 ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt aagcgtcgtg
      181 caaagcgacg cttatttttg tggttgtttg gtacttctgc aggtttgatt actattttga
      241 ttactgtgtt cgtgaccctt tttctaatac ttgcggcggg ttcgattgat aattctgatt
      301 cggattcaag tagtggtggc gaagctttta caggagaata ttcggaagga ttacccattt
      361 ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat gctgtaggtg
      421 cagcagtcaa atataaatta ttaccaagtg tgatcctttc gcaatacggg tatgaatcag
      481 cgtttggtac gtcggcgtct gcaagaaatg atctcaatta ttttgggatt acttggttcg
      541 atggctgttt atttcccaaa ggaactgcgc gtggaattgg tggcattgaa ggtggctggt
      601 acatgaagtt tcccaactca aaagctgcat tttcctatta cggatttatg gtagccacac
      661 aatccaattt taatgcttgt gtcggaaata aaagccctgg tgcaagctta ttgatcttag
      721 gacgaggcgg ttatgccgct gcaggaatta cggaagatag tccgtattat acaggttgta
      781 tgtcaattat cacttctaat aaattaaccg aatatgatga attcgccatc aaacattggg
      841 gtgaaggtgg caataacaac ggcacgatca ctggtgaatg gacgaatcca ttccctggca
      901 gcagtttaga taaaagttcc tttagcggtg gtcaattatt tggaacgaat ccagggggag
      961 agttccgacc gaatggtttc catgacggat tagattttgg ttcagtcgat catccaggaa
     1021 gtgaaattca cgcggtgcat ggaggaaaag tcgtgtacgt tggcaatcct ggtatttcag
     1081 gtttaggtgc atgtgtgatc gtgattaatt acgacggttt gaatatggtg tatcaagaat
     1141 ttgcgaatag tacaggaaat tctcgtgtga aggttggaga tcaagtgaaa gttggtcaag
     1201 taatcgggat acgagataca gaacacttgc atttaggttt cactcgaatg gattggcgtc
     1261 aagcacaagg acacgcattc acagatgatg gtacatggat cgatccatta ccatttttga
     1321 atagcagcaa aaaatagatc aggagaaaaa atatggaaaa taaaacttta aaaatcgtct
     1381 taacagctgc aattagcgtt agtttaatcg gcaatatcgc gttgttttgg cagcttaaag
     1441 aagaacaaga gacaaatacc tttaaaattg aagaagtggc taaaaacgcg tctgagaagc
     1501 tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa accatcaatg
     1561 agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca aaagaggaag
     1621 tcactgaagc acaaagaaag acggcagaag agttcgggca attggccatt gataaatcat
     1681 taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca
     1741 aattaagtcc tgcagacgat gatcaccaac atgatgatta cggaagttac tcctttacat
     1801 taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa aactttgtgg
     1861 tatttgttga ttatacaatt ggcaatcctc aattcaaaga tgtcaaacca caaaaaataa
     1921 aaggtggatt aactgtcact gaaaaacagg tagatggtca atggaaagtc acagacttta
     1981 gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagcaag acaacgtttt
     2041 aaaaaacgaa aaagttatct tagatggctg ggaatcttta tgattctcgg ctttttgatt
     2101 cttgctagtc cgatttacac gcataagaag gtagacatac cagatgaagt aagcacgaac
     2161 gcctttttaa acacgaagga actgacgatg gtttcaaaag aaaaaggagc gaaccagcaa
     2221 ttagtttttg aatttgcgat cgacgatgaa gatcagagct tattaaaaga actagctaat
     2281 cttaattatc gtgtagaagt aaagacagct aaaggggatt atcaagcaat aaaagccaaa
     2341 gtgatcaagg tgtctgatga ttattttgta gtgaagctat cgcatgtacc agaagaatat
     2401 attgctatgc gattaaccat tatccctgaa aaaattgatc caaaggtgga tatgcaagaa
     2461 ccgcaagatc tgatttatta cgttcacgaa gataaggtca aagatcacgt gaaagacgaa
     2521 gattatgaac aacatgcgat caattacaaa gtgaaaggat acaagaaaga acaaaaagca
     2581 gcacaaaaac aaattgaatc tttccaagca accatagatt tgaatgaaga attagtgaag
     2641 aaactaaaag atcagttgcc ttatcaagtt gcagacgatc aggaaaatac tgaaaataag
     2701 attaatagtt accagcaaga aattgaaaca tcgaaaacgc aaatgaaaga tcaagaagag
     2761 atcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaatat ttagaagaat
     2821 aggtggagta aaagaaatga agcgagcaaa cttggtcggc aaagatactt tcgctgagca
     2881 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcaagaagt
     2941 gaatttatct aacacaacaa tatctgagtt gaagaatggt agaaagagtt tagatacctt
     3001 aaaattcaga tcatttgaag ctctatatat agctgcaaaa aaatatttta cggctgaaag
     3061 aaaataaaaa caggaggatt ttatgtgaaa aaagtatatc aaaaatgttt atggatcagc
     3121 tatatcaccg taggtatagc tgtttttttc tttagtttag ccgttatcgt cggtacaatt
     3181 tttctttggt ttatcaataa agatttatca tttaattggt tcatgagtct attgtcgttc
     3241 attatgttga tctttttatg tttagaaatc cgcgaattca tgccttctca ttggcaagat
     3301 aagaggaaag agtttagaga agaaatcgaa atgaaatcga gcgactggat ggaacatact
     3361 tggttaccat tgaaaagaaa tagcaagcga aaagaatttt ggattctaca attatggcca
     3421 ttaggtaaac tttgtagcta tttggctcgt tttatacttg atttaatcat ttactcgatt
     3481 gtagtatttg gaacggctta ttacttatta aatgatccca ataggattgc agcatcagac
     3541 atttttacag tgctaatgac gttgattttt gtcatgctat ttactatttt cactcaaata
     3601 gccatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag tctattcgat
     3661 ttaattattg taaatgcaat tctagcaact ttaataaaac aaatcgaaca atttggaaga
     3721 acaacaaaag gactgaagga atttttaatg acgtttgttc actttgtaaa tgggtacatg
     3781 tatttgtttg aagtgggtat tattctcttt ttattcgggt atatatccac gaaacttatt
     3841 aaacgttcaa ttgaaaaaga aattgagatt gacctgattc cgacactttt tggttatgag
     3901 tactatgttg attttggaag atatgacgaa ttgatcaata caggtattgt tcacacgagt
     3961 atctatgaag cgaccgttaa gggcatcaga actacgttaa aagatggcca ggagtcgaaa
     4021 gcaattgacc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag tctaagatat
     4081 ggtattggaa aattgtcgtt agatggaaga aatattccac aaaacaaacc atacattggc
     4141 tggaaaagtg atgctttaga agtatctaaa aagctttcta tggaagaaga tgagcttcaa
     4201 tgagttcgat caatttgaac catttatttg aagatatcag taatcatttt caacaatttt
     4261 caattgaaga taatgatgaa ttgatgggat tattccaaat agaaaaagaa tacgactatt
     4321 tgacatttat ttgtcgtgaa ggaagaatca ttgatcccaa agaatcaaaa atcatttccc
     4381 atttaagacg attcaaaaat tatatcagta aaaataaaga aactatttca gaaataaaat
     4441 ttgacggaac aacaaactta gaatatttag taagagtcac ctatcaaaga aaccgccgac
     4501 tgattgaaga tgatcaggga gttttaatat gtgctggtga agaatacgat agtctcaaat
     4561 ttactcagtt aaaatttgaa agtattggtc gttctattta ttttgagtca cgaccatttt
     4621 atatccgata taatgatttt caacatttgt ttttacaaga tgaaccaaat ggattaacca
     4681 ttctagaacg aagagggaag aaacaaatta cattatccta cgagagaccc ctcacattca
     4741 tggatggttc atgtgaatct atttttcaaa aaatcaatcc gtttattaaa gaaatgatga
     4801 aagaaaaaga ttcattacct ttcacttggg ccgagattat agaagcaaaa aataaatatg
     4861 aattattgtg taagaagtat ccgtcaaaaa aatttactaa gaaggtaaat aaatatcctc
     4921 tacgtcatac ttatgcgctg attaagttaa aaccaagagt aaccagaaaa cagtttagaa
     4981 gaatagaagc tgcgatcgaa caggaagaca ttgatgtttt tccttcagaa agatttgatt
     5041 ttataaggaa gaacagccgt gaattcgtaa aatctttatt aattagttat gttcaaaact
     5101 gtattttacc aagttttgca acgattattc tatctgattt aatggacatg agtttttatt
     5161 taaatcagaa actagaattt aattttaaaa cagaacgagg attattaaga cagcataatc
     5221 aaattgttga agaatataat ttgaaacaag ctaaaagatt aaagacattc aaattaaaac
     5281 aacatggtaa atacagattg cttgtcaaac atttacaaaa tcatcctcac tttaatttga
     5341 ttaaaacaaa taaagaactc tttatggaag gatcaactat gcaccattgt gtatataatt
     5401 acttagaaaa aatacaacga ggtggttcaa ctatttggaa atatgaacgg cagaagcatc
     5461 gttatacagt tgaaatagag aaaagaaagt atggcaatta tgaagtggta cagtgctatg
     5521 gaaaatacga ttcattgcca gataaacaag aacttgatga gatcaaaaag gttgttagag
     5581 ctattccgta caaatgatat cacctcaagt tct
//
LOCUS       DAPBAB010000117         5503 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPBAB010000117 DAPBAB010000000
VERSION     DAPBAB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.126"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..547)
                     /locus_tag="RD444_002763"
     CDS             complement(<1..547)
                     /locus_tag="RD444_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550076.1"
                     /translation="MNIITRDELIYAYKDEVNLLEVSQYRSSNVITLNIDTVSEFIKL
                     AKELKINHVYYNYLYYSIDNYLIPLDTYDEFSDEINIEVKKHNEVVDSLDFSKPKQLT
                     LLIVLNGTIMEITLNHFWIKESGIPDNETKHSEIQDKFFKDFKLQQEEISRNEHDDKN
                     RLKEYILSDNEFSYMKNQRLRQ"
     gene            complement(652..1215)
                     /locus_tag="RD444_002764"
     CDS             complement(652..1215)
                     /locus_tag="RD444_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550077.1"
                     /translation="MSGYIYGYARVSTRSQELNRQIDLLNEQNCNEILTEKMTGTKAD
                     RPQLNRLKDKVRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHEVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQRDIERAVKLYDSQ
                     IYSIKEITEMTGISKATLYRYLKTNNF"
     gene            1591..1821
                     /locus_tag="RD444_002765"
     CDS             1591..1821
                     /locus_tag="RD444_002765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550078.1"
                     /translation="MLKNLFFKNKKQLTILEKIFYFFVGNKNRMQNKSYYVRERAGAK
                     WRYRKFKDPWIAKNRNTLFKRVFGFFEDIFRG"
     gene            complement(1856..2005)
                     /locus_tag="RD444_002766"
     CDS             complement(1856..2005)
                     /locus_tag="RD444_002766"
                     /inference="COORDINATES: protein motif:HMM:NF025625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDT7550079.1"
                     /translation="MIEKNGVLRTILDISYNSPSRVAELISGNSTNGWSFVEGLSELR
                     TMDQE"
     gene            2080..2343
                     /locus_tag="RD444_002767"
     CDS             2080..2343
                     /locus_tag="RD444_002767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550080.1"
                     /translation="MILQVYNEQQGLTGGSSESSSSPESSEPESAAPSTPAGGDVSSV
                     DTNGNGNVTIKEAKAAGYSMPITSDHWLYQYMNDRDNDGMVGE"
     gene            complement(2443..2958)
                     /locus_tag="RD444_002768"
     CDS             complement(2443..2958)
                     /locus_tag="RD444_002768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550081.1"
                     /translation="MIHVEQGLTDIQVVPTALVDSEWKFIKDLTIYLEENERIFEGKE
                     LYLIRNFSRKGIGFFEESGFYPDFIMWYFNGSQEYIIFIEPHGMLRENLDGNKVQLYK
                     EIKKVENEISLSEGTRPVLESFILSPTPFATLNNPINNPSNYSKGDLNRQHVIFMEDE
                     NYIEQLFESLI"
     gene            complement(3023..3538)
                     /locus_tag="RD444_002769"
     CDS             complement(3023..3538)
                     /locus_tag="RD444_002769"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HDT7550082.1"
                     /translation="MVPLTSEKIFGLAPIIDDQAKILILGSMPGKESLKKEQYYANKR
                     NQMWKIMENTLKLQLPEEYPKRINVLRKHHIALWDVIHSCKRVGSLDSAITEDVPNDF
                     GSFFKEYSSLKIVGFNGNKAYQTFKSKIGFQDFPEIQFIKLPSTSPIPGKNVKSFEEK
                     VVEWSKVVNSL"
     gene            complement(3847..4209)
                     /locus_tag="RD444_002770"
     CDS             complement(3847..4209)
                     /locus_tag="RD444_002770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550083.1"
                     /translation="MADNLLNKNKGKRNLLERKEEVKPQQSFNRSSLFTVNESQQTNG
                     DQKKDVPRKKERGTTTTIRCATDTSHRLNAIVTSFNLDSVNELIEILLDNYEASLTTD
                     ERREIKTLLEVYQRKSKK"
     gene            complement(4251..5087)
                     /locus_tag="RD444_002771"
     CDS             complement(4251..5087)
                     /locus_tag="RD444_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7550084.1"
                     /translation="MAKVITFGNFKGGTGKTTNSAMMAYTLSKLGYKTLLADLDPQAN
                     ATSLYLHTKQRITNNIVTFDKTLMSAIADEDLSDIIIEIKENLFLLPSFADFTSYPLF
                     LEKKFPDSQFNRVTFLNELMTKIKDDYDFILVDVPPTLSTYTDSALLASDYTIIVLQT
                     QERSLVGAEAYVGYLQELIDNYNADFDILGVLPVLLKNNSKVDEATLNTAKEKFGEEN
                     LFENVVKNMERLKRYDIIGIVDPDLDTKYDIHDKRVMGLYKRVTEEMLKRLEEIESRN
                     KQ"
BASE COUNT         1726 a          980 c          844 g         1953 t
ORIGIN      
        1 cttgtctcag gcgttgattt ttcatgtaag agaactcatt gtcgctcaat atatattctt
       61 ttaatctatt tttatcgtcg tgttcatttc tagatatttc ttcctgttga agtttaaaat
      121 ccttgaaaaa tttgtcttgt atctcagaat gcttagtttc attatctggt attccgcttt
      181 cctttatcca aaaatgattt aaagtaattt ccattatagt accattcagt acaattagta
      241 aggttagttg ttttggttta gaaaaatcaa gactatctac cacttcatta tgctttttaa
      301 cctctatatt gatttcatca gaaaattcat catatgtatc taagggtatg aggtaattat
      361 caatagaata gtatagataa ttataatata catgattaat ctttaattct tttgctaact
      421 ttatgaactc cgataccgta tcaatattta aagtaattac atttgaggat cgatattgac
      481 tcacttccaa taaattgacc tcatctttat atgcataaat taattcatct ctagttataa
      541 tattcattaa ctccacttcc tacccgatta ttatataatc caaaaatatc acttcaatca
      601 attattaatt tttcagaatt aaagatgaat aatttcattt catgataaga tttaaaaatt
      661 atttgttttt aaatagcgat agagtgtagc tttactgatg ccagtcattt ctgtaatttc
      721 ttttatgctg tatatttgac tgtcatacag tttcaccgct ctctcaatat ctcgttgatt
      781 aacgcgtggg cgtcctcctt ttcggcctct agcacgcgca ctttttaacc cttctttggt
      841 acgctccaca atcaaatcac gttcaaactg actaaacgct tggaagacgg tcatcattaa
      901 gcgtccgtga ggagtgctag tatcgaagtt ttctttcaaa ctcaccagct tcacttcatg
      961 ttcttcaaag taggtaacga gatcaattaa atctttggta ctccgaccta gacgagagaa
     1021 actttccacg ataaccgtgt ctccaggacg caccttatct tttagtcgat tcaactgagg
     1081 acgatcggct ttggttccgg tcattttttc cgttaggatc tcattgcaat tttgttcatt
     1141 gagtaggtct atttgtcgat tcaactcttg cgagcgtgta ctcactctgg catagccata
     1201 tatatatcca ctcataaaaa atcatccttt gtttttgata ttattgtatc ataaacggga
     1261 gttaaaggtg catacataaa gagagaagtt ttgagactaa aaaggactac tttttaggtg
     1321 aaaaatccgt cctttaaaat cgtctcataa tcgattgttt ttgagacttc aggtttaatt
     1381 ttaactgact taattttttg agttattatc taaaagcttt aagtttaagg agttgattat
     1441 tttctggaaa caataaacca aaatttgttt aacctgttgt gccatataat ttttacttag
     1501 gcacacttta acgtattttt agtttagggg tatgctaata tagtagttag atacttacac
     1561 agtattaagg ctgagccaag ggggaaagga atgcttaaaa acttattttt taagaataag
     1621 aaacagctaa caattttaga aaaaatattc tatttttttg taggaaataa aaaccgcatg
     1681 caaaataagt cctattatgt tcgagaacgt gcaggggcaa agtggcgata tcgaaaattt
     1741 aaagatccat ggatagcaaa aaatagaaac accctattca aaagagtctt tggttttttt
     1801 gaggatatat ttagaggata aatttccaaa taaaaaagag gcccatgcct cttttttatt
     1861 cctgatccat agtccgcaat tcacttaaac cttcaacaaa tgaccatcca tttgttgagt
     1921 ttcctgatat taattcagca actctcgaag gactattata ggaaatatca agtattgttc
     1981 tcaaaactcc atttttttcg atcacttttc catcttcaat gtaactgtca atcacatcgt
     2041 tatagcgaat aaagaaatta ccagtcttcc attgcgcact tgattctaca ggtctacaat
     2101 gaacagcaag gtttaacagg aggaagtagt gaatcttcat cttctcctga atcctcagag
     2161 ccagaatctg ccgcaccaag tactcctgct ggaggagatg tttcctctgt agataccaat
     2221 ggaaatggga atgtaaccat taaagaagca aaagcagctg gctattctat gcctattact
     2281 agtgatcatt ggttatatca atatatgaat gatagagata atgacggtat ggttggagaa
     2341 taaaccaaaa taatgaaaaa tgaaagacca gattaatgaa ctgccccctg tcaagtagac
     2401 agggggcagt tcattcggaa atttagtctc ttttatctgt ttttatatta gtgattcaaa
     2461 taactgctca atataatttt cgtcttccat aaatatgaca tgttgtctgt ttaagtcacc
     2521 tttagaataa ttagatggat tattaattgg gttatttaat gtagcaaatg gtgtgggaga
     2581 tagaataaag gactctagaa ctggacgggt accttctgac aatgaaattt cattttctac
     2641 ctttttaatt tctttgtata actggacctt attaccatct aaattctctc gcaacatacc
     2701 gtgaggctca ataaatatta tatattcctg actgccattg aaataccaca tgataaaatc
     2761 gggataaaac ccagattctt caaaaaatcc aattcccttt cttgagaagt tacgaattaa
     2821 gtaaagttct ttaccctcaa aaattcgttc attttcctct aagtagattg tcaagtcttt
     2881 aataaatttc cattcactat caactaacgc ggtagggact acttgaatat cggttagtcc
     2941 ttgttctaca tgtatcaacg ggttatataa agatttatgt ttcttttagt aggctctgat
     3001 tttcaaatca atacctaaat gtttataagc tatttactac cttgctccac tctacaactt
     3061 tttcttcaaa cgattttacg tttttccctg gaattgggct agtagaaggt aacttaataa
     3121 actgaatctc tggaaaatcc tggaaaccga tttttgattt aaatgtttga tatgctttgt
     3181 tcccattgaa cccaacaatt tttaaggatg agtattcctt aaaaaaagac ccaaagtcat
     3241 ttggcacatc ttctgtgatg gcagaatcta aactccctac tctcttacaa ctatgaatca
     3301 cgtcccataa agcaatgtgg tgttttctta gaacattaat tcgttttgga tattcttcag
     3361 ggagttgtaa tttgagggtg ttctccataa ttttccacat ttgattcctt ttgttagcat
     3421 aatattgctc ttttttgaga gattcttttc caggcatgga tcctaaaatc aaaattttag
     3481 cttgatcgtc gataatagga gctaaaccaa atattttttc tgaggttaat ggaaccatat
     3541 ttttatcccc tttgtagttc agtcacataa gagttaattt tttaaaacgt tatggtatct
     3601 aatccttgta gtatactaga atgttttttt tagctttttt tggtgtctat tgacgacttt
     3661 atcttcgtat atttgaacta aatcctcaaa acgtttggtg tcactattgt ttaaattttc
     3721 aacgtataag tttacaaaag tccttcccat ttttttgact acatcattgt aattgccatt
     3781 atatagcgaa atttacacga ttaaagagac acgtctacat gtagacgtgt ctacaaatct
     3841 acaaatctac tttttagatt ttcgctggta aacctctagt aaagtcttta tttctcttcg
     3901 ttcatccgtc gttaaagaag cctcataatt atctaggagg atttctatca gttcattaac
     3961 actatctaaa ttgaaagaag taacgattgc gttcagacga tgtgaagtat ccgttgcaca
     4021 acgaatagta gttgtcgtgc ccctttcttt ttttcgaggg acgtcttttt tttggtctcc
     4081 attagtttgc tgagattcgt ttactgtaaa taaactggat cgattaaaag attgttgggg
     4141 tttcacttct tcttttcgct caagaagatt ccgtttccct ttatttttat ttaataaatt
     4201 atctgccata ctatcaccct cttctttctt gtatatggta tgaataatgt ctattgtttg
     4261 tttcgagatt ctatctcttc taatcgtttc aacatctctt cagtgacacg cttataaagc
     4321 cccattactc tcttatcgtg aatatcatat ttagtgtcta aatcaggatc aactatccca
     4381 ataatgtcat atcgttttaa tcgttccata tttttaacta cattttcgaa gagattttct
     4441 tcaccaaatt tttctttcgc agtattgagc gtagcttcgt cgacctttga attgtttttt
     4501 agtaatacgg gtagtacacc taaaatatca aaatccgcat tatagttatc aatgagttct
     4561 tgcaagtacc ctacataagc ttcggcccct accaaagaac gttcttgcgt ctgtaggaca
     4621 ataatggtgt agtcggaagc taacaaagcc gagtccgtat aagtacttaa agtaggtgga
     4681 acatcgacca aaataaaatc gtagtcatcc ttgatcttag tcattaattc atttaaaaaa
     4741 gtcaccctat taaattgact gtctggaaat ttcttttcta aaaataaggg ataagaggta
     4801 aaatctgcaa agctaggcaa taaaaaaaga ttttctttga tttcaataat aatgtccgat
     4861 aaatcttcat ctgctatggc agacattagc gtcttgtcaa aagtaacaat attattcgta
     4921 attcgctgtt tcgtatgtaa atacaaagat gttgcattcg cttgaggatc taaatccgct
     4981 aataatgtct tatagcccag tttagataat gtgtaagcca tcatggcact attagttgtt
     5041 ttacctgttc cacccttaaa atttccgaat gttataactt tagccattgt aaccctcttt
     5101 tcttagttgt ctacaaatct acatttgata ttataacgta aatataccat gctaaatcaa
     5161 tatgtcaaac gttatcaaga aaaaccgtct acaaatatac atgtagacgt atctacaaat
     5221 ctacacgtct acatgtagac gcatatacat taaaaatctt gataacaaaa ggatatcaag
     5281 catcatgtat atgcatctac aaatctacaa atctacatgt agacgtgtat acaaatctac
     5341 aaatctacat gtagacgcat ctacagaaaa acgagatttt gcctacaaat taaggtttta
     5401 caaaaaaaaa atagtgtagt acatttgatt taattcaaag caaaactaaa attgaataca
     5461 aaaaaagccc cgtagacgcc aatctacgag acacgcaaaa ctt
//
LOCUS       DAPBAB010000118         5477 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPBAB010000118 DAPBAB010000000
VERSION     DAPBAB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.127"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            10..462
                     /locus_tag="RD444_002772"
     CDS             10..462
                     /locus_tag="RD444_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550085.1"
                     /translation="MLYLKLCNRTVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            476..1165
                     /locus_tag="RD444_002773"
     CDS             476..1165
                     /locus_tag="RD444_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550086.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1290..3641
                     /locus_tag="RD444_002774"
     CDS             1290..3641
                     /locus_tag="RD444_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDT7550087.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3721..4116
                     /locus_tag="RD444_002775"
     CDS             3721..4116
                     /locus_tag="RD444_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550088.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4441..5058
                     /locus_tag="RD444_002776"
     CDS             4441..5058
                     /locus_tag="RD444_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550089.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5099..5398
                     /locus_tag="RD444_002777"
     CDS             5099..5398
                     /locus_tag="RD444_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDT7550090.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
BASE COUNT         1914 a          727 c         1115 g         1721 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg tatttacaat attaattggg
       61 ggctattttc ttttgaattt agattctgtt aattcaaacg ctcaaataaa agatcaaaga
      121 gtagctgaac ataataataa aaaaatgagt ttgaccaaaa atatctattc tatgtcagat
      181 gaagaattgt ataaagaatt tggtgattta tccggggaac agttagaaga aagtattcca
      241 ggaattagtg actcagataa aattgttgtc ttgtctgctg gtgggcaatt aatgggagat
      301 ggcaaatccg aattagttag ttataaacaa aatcacagac agtttattga tgttaatact
      361 gattccaacg ctatttcgat taaggatcta aaagagcttt taaatagtaa aagaaatgaa
      421 ttaaagcaga taaaagaaac taattcagtg gaattagagt aaaggaagat tcattatgaa
      481 aaagattttt ggtgtttctg ttgggctact tatgatgtta acatttggaa atgcagtata
      541 tgctgctgag aaagaagtta tttcagatcc agagcagtac gcctcacgat actttgctga
      601 ttttgaagaa gaagataaag tacttgcatt gccagaaggt ggttttttaa aaggggaagc
      661 taaaattgta tctatggata atccagatat aattcaaggt tcatataatt cagaaactga
      721 tcctgcttca gtttcagtga cagaagcaaa aaaaattgtt gctgaaaaat tcaaaaatga
      781 tattgaaact actccaatta tgcctcgagg agcatttcca cccgatgttg gtactagcgg
      841 acaccttctt gtactagcag gaggaggaag ctataaatca cagccattta gtggtgcggg
      901 atggcgtttt ggaggtatgg gtgtaaaacc agctgatggt acaggcaact ttttacgttg
      961 gaaatcctac attgacgatg gaagagtagg aaatgtaaat caagccaacg caactaaatc
     1021 tggttctatt caaggaatgg cattgtatgc tcgtgaagga ttcaaatact ttgcaccaaa
     1081 cgggcctggt tggacaatga atataggtat ttattacact tataatcctg ttgcaggtac
     1141 gtattatgtt gttcaaaata tttaataaaa cagcacaaag agatttcttt gtgctgtttt
     1201 attattttta gttgttgaca aacactaaaa tataaaatat aatcaacaca taaacgaacg
     1261 tatgttcggc aagaaagcag gggattttta tggatgagat ggttttaagt actcagaaat
     1321 ggctaaacaa gaaatacagt aatgttacag ggtttgataa ggttccagaa aatgggcgaa
     1381 cagggtggcc tacaatctat ggacttattg aaggactaca agtagaactt ggtattacta
     1441 atctagtagc taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta
     1501 aatggggcaa aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta
     1561 aggggattaa tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa
     1621 aagagttgca gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga
     1681 aatgggccaa agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta
     1741 aaattagaaa aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc
     1801 cttgtgatgg tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg
     1861 aactaggata ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa
     1921 cttatcctgt gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat
     1981 atgttaatgg ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt
     2041 tagaagtact ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg
     2101 ttgttgtaat aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg
     2161 cagatacttc tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa
     2221 aaatagttgg tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt
     2281 atctaactac agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct
     2341 atcaagatgg aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta
     2401 agaaggctat agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg
     2461 ctgttgatct agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg
     2521 gggtatctgc tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg
     2581 tttgttctca aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga
     2641 ctggttatag tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg
     2701 ttgaatttac cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg
     2761 gtagagatag cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca
     2821 aatacaatca gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg
     2881 gtcttgataa tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt
     2941 tgtcatggga catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat
     3001 ataggaataa attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg
     3061 gttcaataat aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg
     3121 acacaatcaa tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct
     3181 ggggtgaagt taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta
     3241 cggctgcagg tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg
     3301 tttatacctt agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact
     3361 attgtgataa gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt
     3421 cagataaaat cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct
     3481 taggtgtagg gataatttca tttttcattg ataaaatagt taataattca cttgggacag
     3541 attttactta tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa
     3601 aaattattca atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa
     3661 cagtgtcctt ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac
     3721 atgatgatta tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct
     3781 ggatgtggaa ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact
     3841 gtcaagatag tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct
     3901 gagtttgaaa gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta
     3961 aaagagtctc caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa
     4021 aaaaagaaag aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac
     4081 cctaaattag aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt
     4141 taaagaagtc aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa
     4201 ctggataact agtttattta atggcagacc gttaaggcct gccattttta tgtattttag
     4261 cggaataaat ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga
     4321 catgccttga ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg
     4381 tttctgattt aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg
     4441 atgagaaaat ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg
     4501 atttccgcga aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc
     4561 aaagatttta aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta
     4621 ctctatctac aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg
     4681 cgagaattaa ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat
     4741 actgctagtt acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa
     4801 ttactcagct atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc
     4861 gtagaagctg cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct
     4921 gaatttcctg cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg
     4981 gatcaattgg gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc
     5041 ttgcaccact tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat
     5101 ggacttaacg aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca
     5161 agccatcggt ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac
     5221 aaatttcctg gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa
     5281 aaaggaacaa accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga
     5341 agtcgaattt tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc
     5401 gttagtaaaa tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg
     5461 cattgaaata actagtt
//
LOCUS       DAPBAB010000119         5467 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPBAB010000119 DAPBAB010000000
VERSION     DAPBAB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.128"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            119..1108
                     /locus_tag="RD444_002778"
     CDS             119..1108
                     /locus_tag="RD444_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7550091.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="RD444_002779"
     CDS             1090..1551
                     /locus_tag="RD444_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7550092.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="RD444_002780"
     CDS             1601..2065
                     /locus_tag="RD444_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7550093.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="RD444_002781"
     CDS             2079..2828
                     /locus_tag="RD444_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7550094.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="RD444_002782"
     CDS             2828..3658
                     /locus_tag="RD444_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7550095.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="RD444_002783"
     CDS             3658..4305
                     /locus_tag="RD444_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7550096.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="RD444_002784"
     CDS             complement(4403..5179)
                     /locus_tag="RD444_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7550097.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5261..5404
                     /locus_tag="RD444_002785"
                     /pseudo
     CDS             5261..5404
                     /locus_tag="RD444_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          740 c          973 g         1882 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa ttttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtatcgtaaa gttatataac tttataatac ctattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtattaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgtatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ttaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaga tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaata aaagattaag atataaatat aaatgaacta gtctttgact aaccaatgcc
     5281 cgcttttgaa agaaaacaaa ttcattctgt tttaagtaaa aatccttatg ttaccactca
     5341 ttctgaaggg gatgagcctt atcgctactt ggttgtggaa ccaagcgata agacaatttt
     5401 ataaattcaa tattaattaa aataaaaaca aacaaagtag attattttac tttgtttgtt
     5461 tttttat
//
LOCUS       DAPBAB010000120         5431 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPBAB010000120 DAPBAB010000000
VERSION     DAPBAB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.129"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..222
                     /locus_tag="RD444_002786"
     CDS             <1..222
                     /locus_tag="RD444_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550098.1"
                     /translation="LTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVEGKIKSNQTK
                     KYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            241..1833
                     /locus_tag="RD444_002787"
     CDS             241..1833
                     /locus_tag="RD444_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550099.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1845..2180
                     /locus_tag="RD444_002788"
     CDS             1845..2180
                     /locus_tag="RD444_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550100.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2327..2521
                     /locus_tag="RD444_002789"
     CDS             2327..2521
                     /locus_tag="RD444_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550101.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2553..4925
                     /locus_tag="RD444_002790"
     CDS             2553..4925
                     /locus_tag="RD444_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550102.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            4986..5431
                     /locus_tag="RD444_002791"
                     /pseudo
     CDS             4986..5431
                     /locus_tag="RD444_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2170 a          864 c         1127 g         1270 t
ORIGIN      
        1 ttaaccgaaa tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt
       61 ggtacaaatt caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc
      121 aatcaaacca aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc
      181 agagtagatc catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg
      241 atgaataaat cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct
      301 ttttcaacat acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt
      361 acgcccgaag aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt
      421 cctgaaggag aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat
      481 gaagctcaat cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt
      541 accaaatatc tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg
      601 caaagggaac aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag
      661 ccacataaaa aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg
      721 aaaggaagtg atctgaagga gatcaaacaa atcttccaaa gaggacaaca aaaaggctca
      781 atcatttatc aagatgttat ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac
      841 gatcccaaaa cagatacctt agatgaagaa ggtttgaaag cagctggaag aaaaatgatg
      901 gagacattat tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac
      961 cgtaacacta agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa
     1021 ccgtatactg agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa gagaagacaa
     1081 gcaacattag acgcaatgaa aaccaattat gtacatgagt tagaacgttt ctcttatgaa
     1141 aaggatcgaa tgatcggtat ggagaaacga aatttattga cacgaaaaag tgatttgcgt
     1201 aacaccttaa caaaagtagt aaaagaacca gcacgttacg atcgacacgc catgaactta
     1261 ttaaatgaag tatataaaac cctacctgaa aaacgttcgg aatggaatta tggagatgaa
     1321 aatcgaacca agctttcacc atttacaagg ggaaaacttg atgagttaac caaatatgtt
     1381 ttaacaaaag aacctggata tcaagaatat atacagattt caaaagcatt gaaagaagaa
     1441 gccaaaagcc tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa aaacgccctc
     1501 tttgatttaa aaaagcgcac aggaaatgcg attttaacag aactgaagag acaggatagt
     1561 caaataaaac cattaataga aagtgtcaat cagatgcaat ctatttacaa tgaacgatcc
     1621 tttcgaacac tcaaacagtt gccaacaaat gaatacaagt atagttatca agaattcgta
     1681 aagcgtagga aacaattcca acaaagagtt gtcacgcagc gaagcttgcg tcaattaaat
     1741 cgaacaatta atgcacatga agaaaaatac cacgcaagta aagcatatga agagcaacag
     1801 cgaagaatcc aaatggaaca agaaagacaa taggaggaag agaaatgaaa caatatttag
     1861 atcaatggaa agtcatagaa ggatcactaa aagaagaacg aatcgaacaa cttcctgatt
     1921 gtttagaaaa agagcatttg tttcagataa gcgaaatgtt gagaaacgaa cagtttgatc
     1981 caaatcaatt tttagttgtt gagtacccag ctactggtgt gtattgttgt aatcatgtga
     2041 agggtgaaaa atattttatt attcaagaat acgaaggtaa gttagcgcct tattatacta
     2101 cttgggaaat aaatgaagag ggaatcaata atttcccatg tgaaagcatt gaagagtcta
     2161 tttctctcac ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg tgattgtcca
     2221 ggactctttt tcaatgaatt gttttccgcc aattcactga gtggtgcgat tcaattttat
     2281 tatatcaaaa gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga ctcagacaac
     2341 gattggtgtt gtggttgaaa aaagcaggga taatctcgtt tttgtgacag aaattcaaac
     2401 aggtagagct tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg gcaatatttt
     2461 aacgttaaat ctgactacga agatgttcgt tgatgcagta gaaaattatc catttgttta
     2521 gacaaacgat attttacaag gaggaaacgg acatggcaga agaaaaagaa ccactagata
     2581 atagtcgttt gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa ttaaataatc
     2641 aatttcaaaa gctatttgat caccaaaaat tgacaaatgg gcaaccaatg aatgacaaac
     2701 gaaatggtcg ttcatggttt aaccagcaag aaaaaataga aaataaaata caaagtttga
     2761 gagaagagat caagcaacaa gaaaaacagg tagaaaaact tgaaagacaa gaagagctaa
     2821 aggaaatggg ttataacaag tacggtggcc tggacatgac gatcgaaaat attccaagaa
     2881 tcaaagaaga aatcgaacgc tttgaaaaag gtgaatcgac tttttcagct gccacgatcc
     2941 gaaaatacca acgaaaattg gaaacattag aacagctgaa agaaaaatca gagaaaggaa
     3001 aagaaaatct tcttcctgaa gtacaagcga tcattgacag cggacgagtg acgcaatgga
     3061 aaaagaatcc aactatctat tttctgaaag gctatcgaaa agtcgcttta gaactcaatg
     3121 aaaacggggg atttgaggaa tctaaaaaat accaagccac gtcggaagaa gaaaaggcag
     3181 ctgttcgtga attacttgaa gaagctacac ctactgattc tgtgaaggag aaagaaaccg
     3241 atcatttcta tcgagtagaa ttcaacgaaa atgatccagc aaaagggttg aaaagttatg
     3301 gtggggagat cgtaacacta gaattgatcg atcaactcaa aacactggat aaccaatacg
     3361 ccgcagaagc aggctattat aaattctatt ttgaagaagt gaagaatgga gaagtagtcg
     3421 atcagatacg aatggatcta ggggatggcg tcaatagtaa tcaccccatc tatttagagt
     3481 tagctagatt gtgcgaacaa tcacctgaaa aggaacaaga acaaacagtc aaaaatgaac
     3541 ctatttcaaa agtacaaaac gagattaaag agacaaaaac aaaacaaaag ggttatcaaa
     3601 aacgaacggc agaagagatt aagcaagaag tcaaatcact ttcccaacag gcgcttgatg
     3661 cggtgaaaaa gcatactcat tcacctgaag acgtcaaaga acttttgaat tttatgtctc
     3721 gttttccaga acgatccttt agaaatcaat tattgatcga ggaacaattt ccaggcgcaa
     3781 cggcttgttt aggtcgtgcc gcgttgaaaa aagaaggaat ctatatcaaa aaaggagaac
     3841 aagggaacaa gatctttgtc agaaaaacgg tcaaaggatt ttatgagaaa ggacgtggct
     3901 ttgttcgaga aacagaggct acaccagaag ataaaaaacg aatcgaaagt ggtcaaatcg
     3961 aagtgatcaa aaaaccttac tacacgattg aaaaagtgtt tgatatcaca caaacacaat
     4021 tgaaacctga agactatccg aaaatcttcc cgaaccgcgt ctttgatttt caattggatc
     4081 aaacagggca gacagaactt caagttggga ttcaagcggt agccaaaaat attggaattc
     4141 aaattcgaga tatgacagaa agtgaagtct atcaaagaga attaggacaa gcacgtgggg
     4201 cgtacgttcg taacgaattt actggcgaag aagaaatcgt catgaatact cgaaatactc
     4261 ctacgcagca tttagccaca agtattcacg aattagccca tgctagaatg cacaaatttt
     4321 cagaattaga tactgcaaca aaagaactac aggcagaaat gacctcctat attgtgtgca
     4381 agcattttgg gatggacacc tcggagaaag ctattcctta tattgcgaca tggacgaaga
     4441 atggtcagac gttggatgat aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca
     4501 gtcgtgtcgc aaatgaattc attcaaacca tttctaatga aatcaatcaa tacagagaaa
     4561 gagaaccaga gatccagcca gcagaagtga agcaggagca taaaaatact gtagaaacgg
     4621 cagaacaaaa gaatattgtg cccgttggct cactttggat cgacaagaaa gacgggaaag
     4681 tcctggaaga agacacgggc cgaacgttac atttgaagga tgcggcgttt attgaagatc
     4741 cgcagcataa tcaagtgatt ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt
     4801 taccttcaga agaatttgcg ctaggaaatc cattccgatt ttcacttgag acagtgtcag
     4861 aagtggcaaa acgaaaaaat gaaatctatt atgaacaaca attgtcacaa aaagaacgcg
     4921 tataaaacaa gcaagaaaga ctatctgaaa aatggatagt cttttttctt ttgggaggtt
     4981 tgaaaatgac agagaaaaaa atgagtctca ttgatcgatg caagcaaatg gatattgtgg
     5041 attttgcacg aaacaatggt ttagcggtgg ccaataaagg acgagactat cgcttagaag
     5101 accatgattc atttgttttt gatcgaagaa aacaacgttt ttattggaac agtcaaaata
     5161 ttagtggcga cattattgaa ttagctaaac tttttttcat agacaaagag atccaagacc
     5221 ctaagcaaca atttaaagca gccactgatt ttattctaaa gaatgaagac aaaacagaac
     5281 gagtggaaaa tcttcatttt tagacagaaa agtacaaaga tcaccccgtt gattatcaac
     5341 cactcacaga aaaaggaagg aattatctga aggaagaacg aaaactgccc gattggttga
     5401 ttgattatgc agaaaaagaa ggattgattg c
//
LOCUS       DAPBAB010000121         5390 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPBAB010000121 DAPBAB010000000
VERSION     DAPBAB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.130"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..487
                     /locus_tag="RD444_002792"
     CDS             <1..487
                     /locus_tag="RD444_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HDT7550103.1"
                     /translation="SGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLC
                     NFSNLWLMGINVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEIS
                     LENYKKFAKTTARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYIS
                     E"
     gene            1175..1996
                     /locus_tag="RD444_002793"
                     /pseudo
     CDS             1175..1996
                     /locus_tag="RD444_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            2139..2363
                     /locus_tag="RD444_002794"
     CDS             2139..2363
                     /locus_tag="RD444_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7550104.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            2378..3247
                     /locus_tag="RD444_002795"
     CDS             2378..3247
                     /locus_tag="RD444_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDT7550105.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3228..3965
                     /locus_tag="RD444_002796"
     CDS             3228..3965
                     /locus_tag="RD444_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7550106.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVD
                     FPEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYN
                     EKGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPP
                     ENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3998..4906
                     /gene="ant(6)"
                     /locus_tag="RD444_002797"
     CDS             3998..4906
                     /gene="ant(6)"
                     /locus_tag="RD444_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDT7550107.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKGLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4903..5151
                     /locus_tag="RD444_002798"
     CDS             4903..5151
                     /locus_tag="RD444_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HDT7550108.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(5154..>5390)
                     /locus_tag="RD444_002799"
     CDS             complement(5154..>5390)
                     /locus_tag="RD444_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7550109.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1843 a          809 c         1150 g         1588 t
ORIGIN      
        1 ttcaggttat attcctgata cgaaggctat gcttatttac gatgaaacag ggcaattaga
       61 aaattattta tcagagataa gtggtgcaag accaaataga cttactgaag aaaatgctaa
      121 ttttttgttg tgtaatttct ctaatctatg gttgatggga atcaacgttc taaaaagagg
      181 agaatatgct cgttcattag aactcttatc acaacttcaa aaaaatacac tacaacttat
      241 acgtatggca gaaaaaaatg ctgataattg gctaaacatg agtaaaaacc ttgaaaaaga
      301 aattagcctt gaaaattata aaaaatttgc aaagaccact gctcgattag ataaggtaga
      361 attatttgaa gcctataaaa attctttgct attagttatg gatttgcaaa gtcaccttat
      421 tgaacaatac aacttaaaag ttacacatga cattttagaa agattgttga attacattag
      481 tgaataggag agtaacgtta ttcacagtat tgggtactga aaattaacag catacctcag
      541 tttttcaaaa aacagcgaag cctatggata tgttcccgtc taccgatagt gcaaaaagtg
      601 tcgtggatat gtttccgaga acggacatac acaaatgaca ttcattctgt cctctcaatc
      661 catgtggaag cagtatgtct gttaacaaga aaataattat tgttcgtcct gacgagttgt
      721 tagactcgaa aaggataaac atcattttta gaaccggtag ccgatttttg gctagcggtt
      781 ttttgggctc tttggcaaat agtggtgatt gaatctcgcg ctatcctatg gaaaaccgcc
      841 ttgtgtgagg ggctgcgaga ccgcttcgat cctagaattg ctgaagcacg aatctagcgt
      901 cactcacatt tcatcttttg acgtagctga gacaaggtgt ttttcttcga tccgctagtg
      961 gttaatctct ttttggtgca aatatggttt agaatttctt tgcatggctt aagggagtgg
     1021 agaatcggat attagtagga tttacatcca cagataaaaa taggcttgat tttaacagcg
     1081 aaactaaaat accctactta aatgaagtga tagattagat gttgggttgt taggagatgc
     1141 ttaaaaatcc tatgcaatca atagttttta gtgtttgttg acttgatccc taaatcgcct
     1201 gagtactaaa cgttgagtgt gtctgcttac tgtcactagc cagtaagtga gactgtagtt
     1261 aatctctcat tttccccagt attatgttga aaatagccat gaagcgatta tttctaaaga
     1321 gttatttttg caggctcaag aagaaattta tcggagaagt aatatctaca caggagaagg
     1381 caagaacaaa agaatttata gtagcaagta cgctttaagt gccatcacct tctgtggata
     1441 ttgtggcgat atttatagga gaacctattg gaatattcat ggcagaaaag aatttgtctg
     1501 gcgatgcgtg actagaatcg agcagggtcc cgaggtatgt aagaaccgaa ccgtaaaaga
     1561 agatgaactc tatggtgcgg taatgaccgc aattaataag ctacttgctg gtggcaacaa
     1621 tatgataaag acactggaag aaaatattca tgctgtgatt ggagaaacca cagaatacca
     1681 aatttcagag attaataccc tactagatga aaaacaaaaa gaactaatca agctggccaa
     1741 taaaggtcaa gattatgaat acctggtaga tgagatcgat gagatgcgag ataagcgtca
     1801 gactctttta gtagaagatg cctccctcag tggcgagaac gagcgcatca atgagttgat
     1861 tgaatttatc cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa
     1921 gataatccag aacgttaagg tctatgaaga ccacttcgtc atagccttca aaccaggtat
     1981 cgaaatggaa atatgaaaac cagaagtaaa tagcccatga ctctgaagta gagttgtggg
     2041 ttttctttta attgctgatt ataataatat tttaatgact attgcattta tcaaccaaat
     2101 ggtttatgtt attcttagca taaacttgga ggtgctgtat gactacggca gaaatgatta
     2161 aagaactgtg tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga
     2221 ctccacagaa ttttaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg
     2281 ccattgctga tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagatg
     2341 aaataaaaat atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat
     2401 agtaacagag aaattatcat ctttgccttt tatagaaggc attgtattag ggggctcacg
     2461 tgcaagaagc acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga
     2521 ctcatttgac ctgacagcga ttaatcaaat tgctacagag ttggatgatg agaatagaaa
     2581 caaccttgtt gtacctcccg gagcgtgggg tgattgggtt aatggcggtg gatggttagt
     2641 tataaacggg tgtcatgttg acttgatatt acgtgatata aaacgggtgg aacaaataat
     2701 caaagatacg gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta
     2761 tataagtgct atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga
     2821 aagcttatgc gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt
     2881 gatgaacttt tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg
     2941 ggcagaggat aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca
     3001 agtactattt gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct
     3061 tgaaactttt gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt
     3121 actcggtctt tctctttttg aatgctacga catgaccgag aaactttata aagaagtgaa
     3181 tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca
     3241 aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac
     3301 tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc
     3361 gtgtgcttga tttaggatgc ggctatggat ggcactgtat tatatatgcg atggaaaacg
     3421 gtgcttcctc tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa
     3481 aaacccattt tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag
     3541 aggagagctt tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga
     3601 atttaataaa aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg
     3661 aacatcctgt ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa
     3721 tactgcattt cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg
     3781 aagaaaaggt tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa
     3841 atagttttat aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc
     3901 cggggatggc ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga
     3961 tgtaataata tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat
     4021 ggatttagta ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg
     4081 gtcacgcgca aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt
     4141 tgtaagtgat atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat
     4201 aataatgatg caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc
     4261 ctatcttatg ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga
     4321 gttagataat tacctaaagg gcgataaatt aataaagggt ctaattgata aagattgtag
     4381 aattaaaagg gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag
     4441 ggagtatgat gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt
     4501 gtgccgtaag gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct
     4561 gctgagaatg atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa
     4621 gaactataag tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac
     4681 ctaccggatg gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt
     4741 cagggcggta tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag
     4801 gaatataaca aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct
     4861 ggatagcaca tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa
     4921 gcagggcacc tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt
     4981 ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat
     5041 ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat
     5101 attgacaata ctgataagat aatatatctt ttaacccgtg gtgctagtta attttaatat
     5161 cctaaagaat atctcaaagt tcctccaaaa cgtcgttgtg ctcgacttaa tagaacactg
     5221 taagccagta atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa
     5281 ttttgacaac ctaatttttc taaggaagaa aaaacagtct caactgtttt tctttgtttt
     5341 ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
//
LOCUS       DAPBAB010000122         5260 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPBAB010000122 DAPBAB010000000
VERSION     DAPBAB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.131"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..674)
                     /locus_tag="RD444_002800"
     CDS             complement(<1..674)
                     /locus_tag="RD444_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDT7550110.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
     gene            complement(792..1367)
                     /locus_tag="RD444_002801"
     CDS             complement(792..1367)
                     /locus_tag="RD444_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDT7550111.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            1498..2202
                     /locus_tag="RD444_002802"
     CDS             1498..2202
                     /locus_tag="RD444_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDT7550112.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            2180..2977
                     /locus_tag="RD444_002803"
     CDS             2180..2977
                     /locus_tag="RD444_002803"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDT7550113.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            2979..3878
                     /locus_tag="RD444_002804"
     CDS             2979..3878
                     /locus_tag="RD444_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7550114.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            3896..5257
                     /gene="mgtE"
                     /locus_tag="RD444_002805"
     CDS             3896..5257
                     /gene="mgtE"
                     /locus_tag="RD444_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDT7550115.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
BASE COUNT         1599 a          927 c         1170 g         1564 t
ORIGIN      
        1 ttagaaagca agtgtaaatg gctagttctt gaatggaact gagaatcatt tgtatgtaaa
       61 tgttcaccat taaaaacttg atatgagtta tctgttgttt tacacagctc tgtgattctc
      121 caaaggctac ttgtaccttt tacttctact aaaacaccaa aatcacggtc gcaggcataa
      181 ttgtaaacga ctgcagaagg gtgttttgtg atgcccattt cccgagcaat tttttgatca
      241 gaaaagaata cttctttaac gaagtcagac tgacgatctt ctttaaacat attcaaatcg
      301 ccgccgattt cttcaaatat ggattcagca agttcttgag aatatggtat gtcattacag
      361 ccgactgctt cttgcaattt gatcaaaaaa cggcggcctt ttttctttcc ttgaaactgc
      421 gccgctttgc agtccaatgc tgcagaataa gtcattgaga ataagtgatt acgttcctca
      481 agattgttct gagaaattcc tttttgttgc atgacatccc caattgtctg caaattgacc
      541 aaaggaagaa agcgaaattg catcttttct tttgatggtg tcccgtactc ttcttcgata
      601 aattgtaata actgttgttc catttcgaaa cagtattccc ctaaaggatt aacaaataaa
      661 tagatttcga tcatatgaat ctcccccaaa atagattcta tacaactagt gactagattc
      721 tttcacatcc tataacttaa cagaaaaaag taaaaaaatg aactcaagtg ccttgtgaaa
      781 atgaaaggtt tctaattttt agcttcaagc atacgttgga tcttgttctt agcaggacga
      841 taaggtaaag aaaaacgttt taaaaaattt ataaaagcaa tttttcccac agatgcatcc
      901 cctacctcta gttccagttc aaaatcgtgt agattgtctc cccagctttc atcaatagct
      961 aaaagtcctt cttcaatagg gaattcagct cgttttgttg taagctttcc ggtttggatt
     1021 aatgaagcag gatcgatatt caaatcagaa agtttttgaa aaactgcgcc tgaacgaggc
     1081 agacgttttt cgaagataaa tgcttctgct tcttctgtta taaaggtatc cgttgtttcg
     1141 agacgccctt tttcagcagg tgtttttaat gtatattcag cttgatgacc aacgaaacgg
     1201 atccgtaggc cacaattttg tttcttcagc tgttcatcaa cggtatcgaa ataaatatta
     1261 gtctgtacac ggaaatcttt atctgttaat tgatagtgag cgatgaatat ctcgtactct
     1321 tgctttgtca gcatagattt atattcgatt tcaagcattt gtgtcatttg atcgactcct
     1381 ttttgtctat tttgcctaat tttctttttt tggtcaatgc ataaatcaag agtacgaaaa
     1441 aactttgcat atatggtaga ataaacaatg aatgttaatg aagtagaggt atcagctatg
     1501 gaacgagatt gggaagcatt tttagcacca tatgaacaaa ctgtatcaga attgaaagtg
     1561 aaactgcggg ggatccgcaa acagttccgt gaacaaaatc gacatgtccc gattgaattt
     1621 gtcactggga gagtcaagcc agtcgatagt attgtaacaa aatcacaatt acggcatatt
     1681 ccaatgggac gtttagaaga agaaatgtct gatattgccg ggttaaggat catgtgtcag
     1741 tttgtagaag atatccatca agttgttgag atcatccgta aaagaaaaga catgaaagtt
     1801 ctccaagaac gagattacat cacaaataaa aaagcaagtg gctaccgttc ttatcatctg
     1861 gtgatcgagt atcctgtaca gctgatcagc ggtgagaaga aaattttggc ggaaatccag
     1921 attcggactc ttgcgatgaa tttctgggca acgattgagc attcgctgaa ttataaatat
     1981 caaggcgttt ttccagaaga aatgagtgaa cgacttcagc gtgcggcaga agctgcctat
     2041 cagttggatg aagagatgtc cagtatccgt gaagaaatcc aagaagcaca gcggttattc
     2101 tcccatggaa gaggaaaatc agatgatgtc tactatcgaa cgcttttgaa gaaagaaagc
     2161 gaaagagagg aggcagggga tgaaagtagc gatcgttcat aataaagaag caaagactat
     2221 agaagtgaca gaacgtttga acgcactttt agaacaagca gggattcaac gagatgatca
     2281 tgagccagaa ttggtcatct cagtcggcgg agacggcaca ttattgtcgg cgttccatca
     2341 ctacagtcat tgtctgaatg atgtgcgttt cttgggcgtg catacaggtc atttagggtt
     2401 ttacacggat tggcgggatt acgagctgga agaactagta gacagcttac gaacgaaccg
     2461 ggaacaaagt gttagttatc ccttgctgga tgttcggatc agttatttgg atgaaacacc
     2521 ggaccagcat tttttagcac tgaatgaatc aacgattaaa cgggccaatc gtacgatggt
     2581 agccgatgta tacatcaaaa acgagctgtt tgaaagtttt agaggagatg gattgacgat
     2641 ttctacgcct actggttcga cagcatataa taaaagtgtc ggcggtgctg tgatccatcc
     2701 tagtatcaat gcctttcagt tggcagagat tgcttcactg aataatcgag tgtttcgtac
     2761 tttgggttct ccgatcgtga ttgcccatga tgaatggcta gaaataaaat tggaaaatac
     2821 agaagattat cttgtaacgg ttgatcaatt gaatgtcgca aaagcaaata tccgctcaat
     2881 ttattatcga atagcagaag aacggatcca ttttgcttct tatcgacata tgcatttttg
     2941 gcatcgtgtg aaagatgcct ttattagtga ggattgatat gcagtttaca tgggattatg
     3001 agaaagatca ttctcagcaa gtaaaatatt ttttaaaaga aaaagggatc tcaaagggtc
     3061 tgttagcgaa aataaaattt caaggcggtc aaatcaaagt taatgatcaa gtagaaaatg
     3121 tcttgttttc tctagctaag gatgataagg taaccattgt gattcctgct gaaggagaac
     3181 atgaaaccgt acttttggat gaaacaccga tcgatatcgt gtatgaagat gaacacgttc
     3241 tggtagtaaa taaaccagca ggcgtttcgt ctattcctgc ccaataccat ccaaatggga
     3301 caatggctaa tcgtgtaaag gcttattata aaaaacaagg ttacgaaaac caagtaatcc
     3361 atgtggtgac gcgactagat cgggatacat ctggcttgat gctttttgca aagcatggat
     3421 ttgcgcacgc aaaattagat atgcaacttc gtgaaaagaa gtttgtgaaa aaatatcagg
     3481 ccttagtcag tggaaggctt tcagatttat cggcacatgg acatatcgat ttgccgatcg
     3541 cccgagatta ttcttcgcta ttgaagcgaa tggtctctga tgaaggacgt tcggcacaga
     3601 ctgagtactg gatagagaag cgactaacag aaatcgcgtt agttgatatc cagctgcata
     3661 caggaaggac acatcaaata agagttcatt tttccgcaat tggctgtgcc ctgctcggtg
     3721 atgatatgta tggcgggcga atggatctgg gaataaaaag acaagccctt cattgttatg
     3781 atctgcgttt ttaccatcca tttacagaag agttgctgga gttcaagcag cctttagccg
     3841 cagacatgga ggcagttgtg agacggtttg aagaataagg cgaggtgaga ttggtttgga
     3901 tgaaaatcaa gaattagagg aaagatttgc ccagttgact caagatttaa gggaaaataa
     3961 tattcaggca ttcagaaaca gttttttaga gatgcatatc tatgagcagg ggcaattcta
     4021 ccaatctctt acagaagaga acaggcagct gatctattcc tatctgtcgc ctaaagaatt
     4081 agcggatatg ttcgatgtga ttgaagaaga caacgaacat atgaaagaat acctagatga
     4141 aatgcgtcct agctatgcag ctgatatgct ttctgagatg tatacagata atgctgttga
     4201 cttgcttaat acgctggata aaaagcaaat tgctaaatat ttaagtttga tgtcggcaga
     4261 cgatgccagt gaaatcaagg aacttctcca ttacgaggat gaaacggctg gcgcgatcat
     4321 gacaactgaa tttgtttcga tcgtagccaa tcaaactgtt cgctctgcga tgtacgtgct
     4381 gaagaacgaa gcagatgtag ctgaaacaat ctattacatc tatgtcgtta atcaagaagg
     4441 tcagctagta ggggttattt ctctgcgcga cctaatcgtg aatgatgacg acgcaatgat
     4501 ttctgatctg atgagcgaac gagtgctttc agttcatgta ggcgacgacc aagaagatgt
     4561 ggcacaaaca tttagagatt atgacttctt agctcttcct gttaccgatt atgatgatca
     4621 tttgttagga atcgtcacag tcgatgatat catcgacgtt attgatgatg aagctgctag
     4681 cgattattca ggacttgccg gggtcaatgt ggaagaaatt aatgaaaatc cagtaaaggc
     4741 agcgtctcga cgtttgcctt ggctgatcac tctgttgttt ttaggtatgt cgaccgcttc
     4801 tttaatcagt cactatgaag acttggtaag tgaagcgagt attttagcag tcttcatttc
     4861 gctgattaca ggaactgccg gtaatgctgg aacccagtcg ctagctgtgg ctgttcgtcg
     4921 acttgcgatt tctgatgaga aagatcatgg atttgttcga cttgttttgg ctgaagtctt
     4981 gaccggcgtt gtcacaggag ctgttacggg gatcacgatt tttctaatcg taggtttttg
     5041 gaaacataat tttatcttgg ggtttgtcat agggatggct atgctatttg caatcacagt
     5101 ggcgaatctt gcaggtagtt taatcccgat gctaatggat cgtctaggat ttgatccagc
     5161 agtagcaagc ggtcccttta tcacgacttt gagcgatttg acgagtgtac tgatttattt
     5221 caatattgct tctttattta tgggctattt tatgtaaaaa
//
LOCUS       DAPBAB010000123         5205 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPBAB010000123 DAPBAB010000000
VERSION     DAPBAB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.133"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            71..421
                     /locus_tag="RD444_002806"
                     /pseudo
     CDS             71..421
                     /locus_tag="RD444_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            415..837
                     /locus_tag="RD444_002807"
     CDS             415..837
                     /locus_tag="RD444_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550116.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2007..2975
                     /locus_tag="RD444_002808"
     CDS             2007..2975
                     /locus_tag="RD444_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7550117.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            2992..3516
                     /locus_tag="RD444_002809"
     CDS             2992..3516
                     /locus_tag="RD444_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDT7550118.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            3892..4173
                     /locus_tag="RD444_002810"
     CDS             3892..4173
                     /locus_tag="RD444_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550119.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4175..4591
                     /locus_tag="RD444_002811"
     CDS             4175..4591
                     /locus_tag="RD444_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550120.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1964 a          892 c          868 g         1481 t
ORIGIN      
        1 acaaaggcgt ggttattttg aaacccaaaa agaaaattta tctaggtctc ctcttttcct
       61 tgatgattgg aatagtcaaa ttgaatcaaa aagtcttaat caagatcaaa taataaaata
      121 tgcaaattac tgtggtgttt atcctaaaaa agtagcacga atactaaacc tttcagaggt
      181 ccaaacacaa ctaagaaaag gaaatgccat ttatatggga actgctggtt atggaaatta
      241 tagtaacagc cgtcatgcac ttgcattatt tgggtgggtc aatcaaggtg gttatcaaac
      301 ttactatgtg tggaatcctt ggtataacta cccaactgta ataagtgcaa atgcaggacc
      361 agttacaatt cctgtacctg gaggtggttt tacttgggac aataccatta caaattggta
      421 aataaaccca ttgctttagt tatgggtcta gtgttagttc ttttcttaac tagttgttca
      481 aaaaaacaag aaatacaaaa gactgatctg attccttttc taggaacttg gcaaactaaa
      541 gatcattcag aacgtctagt aatcactaat ttaaatactt caaaaaatat agaacaatat
      601 accatcttag taaaagagaa caatcataaa attcaaatca ttgataaaaa tttaaatata
      661 aatgaacaag ttttcattta caaagcaaca aataatttac taacactgcg ctctaataaa
      721 aaacaattag attacacttt tagtttggat tctccaaaaa agctcgtatt tattttgaca
      781 acgaatgatg ggacagaagg gacagctaaa ccaattgaat tttttaaagc tgattgatga
      841 atagttgttt gatattacaa aacaataatt tcaccgaagg tgaaacaagc gaaactttac
      901 aaaaagtggc tagcatttgc tagccttttt tgtattgtgg agcgtggttt ttgaggggga
      961 ttcaaggggg agcttgcgcc cctttgacaa gcggttattt gcaccaaata cgtaggtatt
     1021 tggctagcaa atatagagct tgccaaacac tttagtgagt tttgtagaaa gtacctttaa
     1081 aggtactttc acattacgaa cgatacgtcc gtaggacgta ctgaaagggt gggatttatg
     1141 agcgaactaa tgcaaaattt ggctagcaaa aaaagttaaa aaatatatgc taataatcga
     1201 gcgaaatgaa cactttttca agtgagcgaa tccgccatga caaagctgaa gcagtttatt
     1261 tcagctttgt catggcgtgt gcctagactt ttaaaaaagt cggcacacga gcaaatatct
     1321 ttatcaagct ctaatttgcg ttttaagacg tttgaaacgt ctaagtatat aaatatataa
     1381 gagtaggttt aagagtgctt aaatcgaatt ttagagggca ttcagacaac tttagggttg
     1441 ttagggcttg ccctgaccgc ctgtaaggcg ctttaagaac aagataaggg atttatacta
     1501 agactgtctt aaaacgtctc aaacgaaggc tcatagcttc tcaaagagtt ccttatgaac
     1561 atttttagca aaggagccgg aagcgactgc gcagtataat gtgagagcag agcgatcaca
     1621 cctttttaag cgactgtagg gagtaagatt cccagttgag aaaaatgcgc gaatctgttt
     1681 ggagaaaaaa gaagtggttt tttaggcggg aaattttgtc cccctctttt ataatctctt
     1741 tttaaacctc tttttaaacc tctttaccgt cggctatatc cttactctcc caagggaatg
     1801 acgcttttat ctacatcgtt ttgtctgttt atctacatcg ttttgtctgt ttatctacat
     1861 cgttttgtct gtttatctac atcgttttgt ctgtttgtac attgaattag tacatcgacg
     1921 atgttaatat aagatgtaga taaacaggca ttttgtattt cataacgtgt atctcttgtg
     1981 cgagtaagaa aggagctatt ttaaatatgt ctaatgaaat cgtaaagtat catcatgaat
     2041 taaatacaat tccgttgcga aaatttacac cagtcgaaat gaatttgttc ttttctatcg
     2101 tatcaagaat gcgtgatgta ggtgataaaa aagtacaatt tacattcgag caattaaaag
     2161 atttaagcaa ttataaagca actgcaaatg ttcgattcat agatgattta gaaacaacgt
     2221 atgacaaatt aatggattta cgttttggta gaagaagtgc tgacggtttg caacgtgaac
     2281 gttttgtttt atttaatcaa tttaaaatcg acggaaaatc tgatattcct tttgctgaaa
     2341 tacaagttca tgaaaaagcc ttacccttac ttaataatct tgaagaatgg gtgcgctatt
     2401 ctctccaaca attcaatgaa ttagaaagca gttattcaaa aacaatgttt cgattactaa
     2461 aacaatacag aaccaaaggt ttcgcttatt tttcaaaaga agactttcat gaattacttg
     2521 atattcctaa aagttacaac caaggtacga ttgatcaacg tgtgttcaat ccaatacgac
     2581 aagaactaac cgcaatcttt aaaggtttaa caatcaaaaa gaaatacgga aaaggacggg
     2641 gaaagcctgt cattggctat caatttacat ttaaacctga gataaaaaaa tcagacgact
     2701 ttaacaaaca tgccccaaca aaagaagaac aaaacaaagc attagaacta gctaaccaac
     2761 aagttcaatc tgaaaaagca cctacttcat tacaagaaaa aatcaaagaa caaaagcgaa
     2821 aattggaact tttacaacag ttagaaaagt tagaaagaga acaaaatacc caaaataaag
     2881 aaaataatgc acaaaaatca gagataaatt ccgatattcc ttcagaacta aaaaaagact
     2941 tattagacaa tcttcaaaac ttattcaaag attgaggggt ttttagattt tatgacagaa
     3001 aaaacaatca aagaacttgc tgaagaatta ggagttagta aaacagcaat taacaaaaaa
     3061 gtttctgacg aaaatcgaaa actttggttt tcgaaaatcg gaaacaagtt tgtgattaat
     3121 gaaaccggac aaaaggctat aaaatcaatg ttttttaacg aaaacgaaaa ctcaaatcaa
     3181 caactagttg gcgaaaaaac gaaaaccgaa acccacaaaa acgccaacca agttcccgaa
     3241 ttagaagtcc tttctctcct agaacaacag ttaaatcaaa aagatatgca actacaagaa
     3301 aaagacgagc aaataaaaca aatgcaaaaa ttactcgacc accaacaaca actcaccctg
     3361 caagccaacc aacaaatcga gaaactgcag gaacaactac agttgactta tacggaagaa
     3421 tcttcggaaa ataaatcaac gactctttct gaagaaacag aatcgataga aaaacaatca
     3481 aagacggaga aaaaatggtg gcaattctgg caataaaacc agcaatttaa attaataaaa
     3541 attgctggtt ttattcttct ttttgctttt gcgagcagag caacatggac ccatgttgcg
     3601 atattttggt ggcgccccaa tccccgtttt ttcgttgaat cttactcgga ctgcactcgt
     3661 tttacagctg atttttctga attttgaaag agcataagga aacgtggtat cactttctta
     3721 cgaaaggtgc tagataggct gtgcctatca cgtttctcag aaaaaaattt gctatcactt
     3781 ttttgccatt gtggctaggc tatgtatagt gttttggcga aaaatagcta tacaaatact
     3841 atatttgtat ggtatacata aattatacgc tgatacgaaa ggaaaatttc catggacatt
     3901 ttagttcgaa atatcaatcc caaaattatt gcaagaatag ataaacgttg tcaggaactc
     3961 acaaccaaaa cgggcaaaaa atggagtcga aataaatatc taaaagttct aatcgaaagt
     4021 gattttgacc gtcaacttat gaattacaaa aaagatcaat tcgatttatt actcgaaaaa
     4081 tttattgcca ttcaaacaga aaatacgaac atactgaatg actatgttca cacaaacaac
     4141 caactcattg aaacacttat cggaggaata taacatgaga aaaacttctg ttcaaaaaca
     4201 cgtaaccctt tatcccagtc acgttgacgt tttagattcc atcatcgaaa aaaatccaga
     4261 cgtcaacaat tattcagaag ccattcgttt tttatgtatg agttatgaac ctgataccaa
     4321 tacaaccaca accagtaacc aagcaaaact caatgccatg ggaaaagaaa tttctatcat
     4381 gacagaaatc atggcagaat ttgctgattt atatctaaaa gatactggtg ttcttatcgg
     4441 tacaaattct aaagtgtatc aagaagtgaa agaaaaaatt gaaaatcaac tcaatccttc
     4501 ccgacgaaat caacccaaaa atcgtcaaaa agaaaccaat caaaccttta cttctggaaa
     4561 ttttgaccca acaaactttc taaaaagata aaaaaagaaa ccgatcccta aaaaaattcc
     4621 tcgatttctt tttagggatc agtcttccag caagatttgc aagttggtac caacttgcga
     4681 aaaaaaagaa aaatccgctt atgcaacttc ttctttttgt attttggggc gctaccccaa
     4741 tccccaagta tcggttattt aatagcgcgg aaatccaaag aataggacgt accctgaaac
     4801 aaaaaaagac tgctctcacg agcagcccaa aatgagatta tcctattcat caacataaaa
     4861 atattacaaa gtgaagaact agagaaagac aaaaaaagca tatcaaatcc tctcaaaaat
     4921 aacaagtctt aaaaaaatga cagataaaaa tgcacaaaga attagatata gataaccgcg
     4981 aaaaatcaag ctttagaaag ttgcttttag ctagaccaat taaaaatcac aaagtgagcg
     5041 tctctatcca ttggtagagg ggctacttgg catagtgtac attatgttaa ctagctcgat
     5101 aaataacgat tatgttccca aaacaatatt ttatgaacta taacaaaaat ttgttatcgc
     5161 atattgtata ttttaattat ataattatag gtgtatacca cttaa
//
LOCUS       DAPBAB010000124         5190 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPBAB010000124 DAPBAB010000000
VERSION     DAPBAB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.134"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            137..748
                     /locus_tag="RD444_002812"
     CDS             137..748
                     /locus_tag="RD444_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7550121.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1752
                     /locus_tag="RD444_002813"
                     /pseudo
     CDS             821..1752
                     /locus_tag="RD444_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
     gene            1821..2647
                     /locus_tag="RD444_002814"
                     /pseudo
     CDS             1821..2647
                     /locus_tag="RD444_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2695..3873)
                     /locus_tag="RD444_002815"
     CDS             complement(2695..3873)
                     /locus_tag="RD444_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7550122.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3971..4069
                     /locus_tag="RD444_002816"
     CDS             3971..4069
                     /locus_tag="RD444_002816"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550123.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4100..4501
                     /locus_tag="RD444_002817"
     CDS             4100..4501
                     /locus_tag="RD444_002817"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550124.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4708..>5190)
                     /locus_tag="RD444_002818"
     CDS             complement(4708..>5190)
                     /locus_tag="RD444_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7550125.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          880 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact cttgatcctg atgcaaagaa tgatgaagaa
     1321 cgctatcaaa gcttcatgaa aaacttagat cgttatctaa gtgagcaagg ctataaacat
     1381 actcatttta ctgacgctgc tggctatgac tccaatgcta caacatcagc ggaagatttg
     1441 acaaaagtag ctgcaaagct actaaagaat gataaatttg cagaaatagt tgccaaggaa
     1501 acttatagag caaaatcaat taagggaaat gtttataact ggaatactac gaatcaattt
     1561 atgcaacaag ataatagata ctttactgaa ggtgtaaaag gtgtaaaaac tggatcgttt
     1621 gatgggagat acaatctatg tgtgtattat acatctagtt ctgataacca ctatttactt
     1681 gtaagttttg gttcagagag tgatagtagg cgttatgtag atgttcaaaa tctattgcat
     1741 aatctgcctt gaaatttcgt gcctttaagc gcgtatctaa agtttctttg aaaagtttga
     1801 gaatgaagaa agtgagcagt cgaaaaacgg ttgctcactt ttttgcccta aaatcgaaaa
     1861 aatataaaaa agtcctttac aacacgtctc aggaaaaact gctaagtcaa ttctaatatc
     1921 ttgtggagca agacttgcac cttttgatat aagagagctt agagaactaa tgagcgaaga
     1981 tgaactagaa ctcgataaaa ttggagatag aaaaactgcc ttgtttgtaa taatatcaga
     2041 tacagatgat acatttaatt ttgtagtttc aataatgtat tctcaattat ttaatctact
     2101 atgtgataag gcagatgatg tgtatggtgg aagacttcca gttcatgtta ggtgtctact
     2161 tgatgagttt gcaaatatcg gtttgattcc taaatttgaa aaactaatag cgacaattcg
     2221 ttcaagagaa atatcggcaa gtataatact gcaagcacaa tctcaattaa aagcaattta
     2281 taaagaccat gcagatacaa tagttggtaa ctgtgactct acactttttc taggaggaaa
     2341 agaaaaaaca acagtaaaag aattatcaga aaccttagga aaagaaacca tagacctata
     2401 taacacatca gaaacaagat caaatcaaaa atcttttggt ctaaattatc aaaagactgg
     2461 caaggaactt atgagtcaag atgaaataac tgtaatggat ggtggtaagt gtatttacca
     2521 gctaagagga gcaagacctt ttctatctga taaatttgat attacaaagc ataagaatta
     2581 caaattgttg gaagattatg ataagaaaaa cttgtttgat gtagaagagt atttaaaaca
     2641 aagatagcgt aaaatatttt gtgtaaataa ctcgtccttc tgcaaaataa tgggttactc
     2701 agtaaacatt gaagctaatg tatccgtcac ttgttggaag cctttatggc ttctatttaa
     2761 gaatttttga ttgtatgtat caaagatgga tactaggaaa cgctctagtg attcttcatt
     2821 ttgaaactgc tcttttctgt ggctgtattt cttgatttgc ttattaaagg attcaatcaa
     2881 gttcgtagag taaatagtct tgcgtatggc taatggaaaa tcatagaaag tgagcaggta
     2941 atcgttcgaa aggatcgatt ccaccatttt tggataactg gttttccatt tttccgcaaa
     3001 agcaccacgt gcttccaatg ccgccttttt agatgacgct tgatacacca ttttaaaatc
     3061 atcacagact tccttacgat catcgacacg cactttgtga ctgatattac gggaaacgtg
     3121 cacacagcaa tgttgaaaac gggctttggg gtagaaccgc tgcacggccc ctgccatccc
     3181 ctttaagcca tccgtgatga aaaggaggac gtttttcacg ccacgttcct ggaggtctat
     3241 taaaatctct tcccaaatcg tgatagattc agttggtgca atcgcatagc tcaacacttc
     3301 ctttgaaccg tccgggcgaa tgccaactgc gatatgaatg gcttccttgg cgacggtttt
     3361 ccgctttaaa gggatatagg ttgcgtccat ataaatagcg gcataacggt catgaagctc
     3421 acgttcctta aatgaagtga cttcttccgt aaatgattta gtcatattgg acatggtttg
     3481 gggcgtgtag tgatgcccat acattttctc aatcaagtct gcgatttccg acatggtaat
     3541 accttttcgg aagaggtgaa tgacggtctc ctctaaggta tcattcgtcc gtctataagc
     3601 aggaacagtc tgttgcttga actcgccgtt gcggtcgcgc ggaatctgga gatgaagttc
     3661 cccgtactcg gtcttgaccg tacggtcata ggagccgtta cgagaattac ccgtgttaaa
     3721 accaatgcga tcgtactttt cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt
     3781 attgacagct ttttccaaat ggacacggaa taattcattc aaatcgcctt tattcgctag
     3841 agtctttaga atttctgtag taaaatcgtt catatggaag tcctcttttc tgtgaatgtg
     3901 ttgtggtaac tctattctac tgaagggact tccttttttc tatttacaca aaatatttta
     3961 cactctcgga ttgaaagaac gcggtctttc aggagtaaag atggtcatct cggatgcgca
     4021 tatagggctc acaaaagctg tgaaagaata ccttctttgt gcgagctagc agagatgcca
     4081 agcacatctc cttcgcaaca tttccgacaa attctccaag aaaatctcaa gcgatatcaa
     4141 agaagacttt aaagccatct tcaaaacggc aagcatcgag ctggctggag aacgtaaaca
     4201 acttattatg gataaatatg gggaagacaa gaaagtcgca actgcctgta aaatattgga
     4261 tgagggcttc gaagatgcca tgcaagtaat ggttttacca gaaaatatcc gtcaacgatt
     4321 gagaacaacg aatgttcttg aaagactaaa tgaagaagta cgccgtcgtg aacgtgttgt
     4381 ccgaattttc ccaaattgtg attctgttat tcgtatcatc ggtgctttat tgatggaaaa
     4441 ggatgacgaa tggacttctg cacctagaaa atatcttgaa tttgatcgta acgagattta
     4501 gcacattaag ccgccaatag tataatatac tggtgagaag tatcaagaga cgcttcgctt
     4561 tttgctcttg acacttctta ccagcatatt ggaccgtagc taggctcaac aaggcaaaac
     4621 atcgtacttt gagatttcct ttttacacaa gattttggac ttgacacagc ccctatggtt
     4681 cttaccttgt ttatgcttat ttaaatctta ctttattaca gtactttcaa acttcgtttt
     4741 gtaagccggg tattttgata tttcataatt atgtccctct ttttgaatat attctttgat
     4801 atctgcttgt tcatctacac tttctttgcg taaaatacgt tcgatcattg agcagcagtg
     4861 catgtaaagc aagaacaatt gattgtttga aaattcgatc cccattagtt tttcgagttc
     4921 tttgacggcc ttttgtacca atctcaaaac cttcattgca tcaaggatcg ttaaattttc
     4981 tacgatcttg ttaatcgaga aattaaagta ttgagcacaa ttataggcga taaatggagc
     5041 atttgattct atcacctgtt gttccttggc gtattcataa attttttctg ccaaaaaggt
     5101 ttttcctgaa ccgcttacac ccatcaataa aatcggtaat ccgttatgag gatacaagac
     5161 agccgtcttg atttgatcga tcgcttcctt
//
LOCUS       DAPBAB010000125         5188 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPBAB010000125 DAPBAB010000000
VERSION     DAPBAB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.135"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(439..1524)
                     /gene="xerS"
                     /locus_tag="RD444_002819"
     CDS             complement(439..1524)
                     /gene="xerS"
                     /locus_tag="RD444_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDT7550126.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVTTITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKNTLSKRALIYYHRDVERDLAINALILGSGLRLSE
                     VVNINLDDLSLDKNKVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            1857..2090
                     /locus_tag="RD444_002820"
     CDS             1857..2090
                     /locus_tag="RD444_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDT7550127.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDVAISQALTEYDTTFRELVDK"
     gene            2087..2494
                     /locus_tag="RD444_002821"
     CDS             2087..2494
                     /locus_tag="RD444_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDT7550128.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLVVYIATYDKDFDQLKYEVINTIKTHTKPI"
     gene            complement(3631..4149)
                     /locus_tag="RD444_002822"
     CDS             complement(3631..4149)
                     /locus_tag="RD444_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDT7550129.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1717 a          838 c          785 g         1848 t
ORIGIN      
        1 tttatttaaa aggtaatgaa tagcttcttc ataagacata tttaataaag aattgtaacc
       61 tattttttta taattgtttt ctgacataat tataaaccaa gctccttact attttaagaa
      121 tagagtatga gaaatatatc ctatcacttt agagttggaa tttacttaca aaaaacatat
      181 aatcccttaa tattatacgc ttatttaatt ttcaatttct ctatattgaa attcaatgaa
      241 gttatcaata ttcctataaa ccacctctac ttgccagtaa ttataattta ttatgaatta
      301 ctcaatagtt aatcatattt attaacaaaa gattattggt tacaacaaaa aaagaaccat
      361 gaatccattt acgttaaaag gattcatggt ttcattcatt attctcactt tactattttg
      421 gctatttata tattatactc ataaagcgtt caatgcatca atggtagctg cattatctat
      481 atgagtatat aaagttgttg tatttgtact tgaatgtccc aattgttggg cagtcaatac
      541 caaagaattt gtctgttggt agagtctagt agccaatgta tgacgcagtt tatgcggact
      601 gacttgtact ctgaatcctt tcgaatattt tgctaccatc cgttcgattg caataccgga
      661 tatacgtttg gcttctcctt ggtaaattgc tagaaataat gctttttcgt tttctgtcac
      721 tttatatcgt tctttccgaa tagctagata attagctaaa tattccattg cgaatgctgc
      781 tatattgacc gcatctctct tatttccttt tctagttact accaccttat tcttatccaa
      841 agatagatca tccaagttaa tatttacaac ttcggagagt ctcaatccag atcctaaaat
      901 cagtgcattg atcgctaggt ctctttctac atctcgatgg tagtagatca aagctcgttt
      961 gctcaaagta tttttatatt cattggcaat ataatctaga taattttgtg tatcctcatc
     1021 tagaaataat tttttctcaa tggcattggc acgtgcagct aacgtagttt tatctgtgat
     1081 ggttgttact tggtccatag gattattgtc taagtatggt tttcctaggg gattattaga
     1141 acttttcgaa agatatttaa atagaacctt taatgctgtg atgtttctat tgatagtaga
     1201 agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct ctttttttac
     1261 attttctttt gtgcgacgtt tcaaatgaga tttgtacaag tttaggtcat aggcagttaa
     1321 ctgtcttatc gaggtgatag aagtatcagg aacgatagac tcactcagaa gccattcata
     1381 aaagagctta taatttttta gatattctaa agtggttcga ggtgattgat ctttatcctc
     1441 acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg tatctatttt
     1501 ttgatatatt tctgtatttt tcatgtcatt catcctttca ataagtaggg gagaagagta
     1561 tctctataat tggactatac caattataga atgttttgtc aattggtatg ctctaaaatt
     1621 tattaatttg tttcaaaaaa agcatataaa taggtatacg tttattaatt taaaataaat
     1681 ggtatacata aatagggaag gaaatagaag ggagcaatct aaattttttg aattttctta
     1741 attaattata tagaaagaac aatctttcta tagagttaga catgagaggg gctatgcttg
     1801 atttaaaatc aacaagcgta tatactgtat atacaaagaa aaagggggag aagactatgg
     1861 caattaaaga aagaaaattt agggaagtgg ggaactcagt agtattaact atttcaaagg
     1921 aatttcttaa agaaaatggg ttaaatcccg gagatacggt ttttttagat gaagaaaaac
     1981 ttcgagccgc aattacaaaa aaacaagcct tatctgatga aattgatgta gcaatatctc
     2041 aagcattaac tgaatatgat actactttta gagagcttgt cgataaatga gtattcatta
     2101 cctagaagcc aaagatatta taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa
     2161 tgaacccctt ggtgtaaagg atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc
     2221 ctcagctttt ggagaagagg tttatccttc gatttatgaa aaagcagcta tcttacttat
     2281 acaattgata aagaaacatc cttttcataa tgcaaataaa cgtactgctt tcttagctac
     2341 ttttgtcttt ttgaaaatca atggacagct tttagctatt gatcaaaaag atgctgtaaa
     2401 tttagtggta tatattgcca catatgataa ggattttgat caactaaaat atgaagtgat
     2461 taacactata aagacacata cgaaacccat ttaaggagtt gctgtatgtg tctttatagt
     2521 gttaatagct gctatagcga aataattgaa gtaaattaca aatagctata atccaaatcc
     2581 aagtatatag aaaacgtaaa ttttctatat agttggatta agataacgac tatttatttt
     2641 atcatatttt tactataccc tatactttta cttggtatat ttatgaataa tcgcaaattt
     2701 acccctatac tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt
     2761 tattaaatac acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa
     2821 taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt
     2881 atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcggt
     2941 actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat
     3001 ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag
     3061 aatttattta agatctcttc agtatcagta ataggatagg cgataggcca catacccttt
     3121 gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa
     3181 ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc
     3241 taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga
     3301 gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat
     3361 aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat
     3421 ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta
     3481 acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg
     3541 cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct
     3601 ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat
     3661 ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc
     3721 ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc
     3781 ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc
     3841 ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc
     3901 taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc
     3961 attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg
     4021 tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc
     4081 taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt tatttttata
     4141 tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca tctataattt
     4201 catccacgat attttaatcc gttctacaat gcatatctga tatgcattga ctgtctaact
     4261 catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag
     4321 caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg gtttgcatat
     4381 cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat aaaaactacc
     4441 actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca aaatccattg
     4501 ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca gcatcgctct
     4561 ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga tgacacgttc
     4621 cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga agaaaattta
     4681 attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa aggtcaagat
     4741 ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca atgcatttat
     4801 aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat gaaacagata
     4861 cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta tgttaatctt
     4921 ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt taaggttggc
     4981 aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa gttggttgac
     5041 acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca gtacctaaaa
     5101 agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc atttcaactt
     5161 atatgtttta gtgaactacc cccactta
//
LOCUS       DAPBAB010000126         5100 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPBAB010000126 DAPBAB010000000
VERSION     DAPBAB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.136"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            13..207
                     /locus_tag="RD444_002823"
     CDS             13..207
                     /locus_tag="RD444_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550130.1"
                     /translation="MIFAAAAAVASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVIL
                     AAASIVVSLFGLFGQTPQGL"
     gene            277..2331
                     /locus_tag="RD444_002824"
     CDS             277..2331
                     /locus_tag="RD444_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550131.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            2331..4940
                     /locus_tag="RD444_002825"
     CDS             2331..4940
                     /locus_tag="RD444_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDT7550132.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            4937..>5100
                     /locus_tag="RD444_002826"
     CDS             4937..>5100
                     /locus_tag="RD444_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550133.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWG"
BASE COUNT         1904 a          799 c         1042 g         1355 t
ORIGIN      
        1 aaaaacaatc tgatgatttt tgctgctgct gcggccgttg cttccattgt tattgggggt
       61 cttttcttcc tatttggacg aagaggagct gaaaatggga agatgcacgt aggctatgta
      121 ttgatggggg taggagttat tttagcagca gcttctattg tggtttcgtt atttggattg
      181 tttggacaaa cacctcaagg attataagaa agtaaaaaag taggagtaag aagtagtcga
      241 tactttttac tcctttttta tgaaaggaga atactcatgt actttacgat tgcaggcaat
      301 accaaaacag ataaagagtt aataaaaaaa gcaacgaagg aagtaggaaa gcgtacttct
      361 tttttaccga aaacaattaa atattctgtc attgaaacgg aagaacagct acatgtgtta
      421 gggaaagtac tggaacacga aagtcgatct tctgttaagt tgactctcac gttacatgac
      481 acagaaggaa aaatagagaa agctggtaca gaatacgttt ttggcccact ggattatcaa
      541 atttcagaag gtttctatcc tacattgatg gatttaatcc gccttgatgt gtttaaagaa
      601 acggatgaaa ctatgagtgt ggaagagtct tatcgaaacg aaaaactttt acaagtttta
      661 gaagaagagt tagagagcga ccaaagaaaa gtgaatcaag ggagtgaagt cgaagtaaat
      721 gaagaagtac catgggaacg accaatattt ccaaagaagg agtcaaaaga aaaagagaca
      781 gtctatgttg acgaacaagc tgatattccg actcagaaat cgatatttga ggaagagaac
      841 gatgtgatgg gtcaattgga ggtagatccc tttgaagaaa caatggagga tgaagagacg
      901 gccgaagtag aagttgatcc agttgaagcg aatgaagagt caacatggac tgaaggggat
      961 tctcaggaat atattgaacc acttatcagt gaagaagtag aaaaaaacga aatagagata
     1021 caaaaggcaa ctgtatttga ttatattttg gatcaatatc cgccagagca tgaatggatg
     1081 aaagaaaaaa ataaattatt tttaaatgag ctttattcga aatatcaatt accccaaacc
     1141 tatcaagcat ttttagagag taaagaagaa ttaatctcac agggtaaagg cacactttta
     1201 gaacagttag agtctgttca atcaacaaat tggcatgagc ttgcacaaga agatttatta
     1261 gccacttttg aagaacgtca acaagaaaca gatcaagaaa taaatgagta tgcgacggaa
     1321 cagtcatcta attgggagag aacaaaagag gaactaaata aagaagaaca acgagtgatt
     1381 gaagaagaag tacaaaaaat taaagagcga tacgaaaaga aacgcgacaa tgcgtattcc
     1441 ttgtgtatag agaaaattaa taactttact aaaactaatc aagaagcttt atcgaaagaa
     1501 aaagaaaact tgttaaaaga acgtataaaa gaacgaaaag aagagttcta ccgaggttta
     1561 cgtacagata agctaaagat tagcaaagaa ttaaatgaac aactgaataa actttatcaa
     1621 acaacgactg aagttcttat ggaaagacac gggcagatcc agcaagaatt acaagcacaa
     1681 attccaaaat ggaaaaaaga gtatgaaatt caacaacaag aattgaaaga acaagaagaa
     1741 aaacaacgac aagaagaaaa agagcgcgaa caattagaac taaaaaaacg tgagcagcat
     1801 cttgcagaac aaaaagtggc gatagaaaaa caacggttag agcaagaaaa ggaaaaagaa
     1861 gcagctgctc gtaaggaacg tcaagaacaa atggatacat tgcgacaagc acagttgaat
     1921 tatctagcac aacctcaacc agtagctgct gttcaaacgc cagtgaacaa ccaagcaaat
     1981 ttaactcctt caccacagcg ttcctttaac ggatggatgg tgggatgtat tgcaagttta
     2041 agcttgcttc taggtggagg aacagtattt gcttttaatc atatcaatca atcttcacag
     2101 gcagattctg tagccacaac ggcgtcaatc aaggaagaag cccaggaaca agcaagaaaa
     2161 gagtatgaac aacaattgga agaattattg aatcgtacca atcagtcaac agcagagtca
     2221 agtatggaaa atagcaaaga gagtgatcct agtaaggcta aagaaggatc aagcgattca
     2281 actgtacact caaacacttc cgaaagttca gaatcaaatg aggtgaaata atgcgtatta
     2341 gagaaacaac gagacgaaaa gacaaacgat tcaatttaga aaagaatagg tatctttctc
     2401 atttctatcg ctatactcct tcaggagaag agctaccaga aaaacatgac aaacgtattt
     2461 ctcaaaagat tttttatagt ggcttagctt atcttccttt aaactatgtt gttacaagaa
     2521 taccagaagc atttcatcga tttgagatta ttccaggaag tttacaagga gtgaatattt
     2581 ttcagttaca agaaatgccg cagttattcc attttgattc aactggtttt tggaatcctt
     2641 tgaccccaac aacaagttta ggcacacgtt tagttgtttc tacgatttgt gttgttccag
     2701 ctgtacttat gggattgaaa atggacatgt ggtttcgtcc gctagcagat gggcaaaaag
     2761 gagataaccg attaaaaacg ttaaaggaaa tccaacaaat ttatccgcaa ataccagaat
     2821 caaagggaag ttttcctggt tatggcggaa taccaattac tcattataaa aaatactggt
     2881 atattcagac ggatacagta aatacttgta ttgttggtac ttcccgctca gggaaaggcc
     2941 agatagaagt tttagcaacc attgacaatt tgtctcgtgc agaaagacaa agttcaatgg
     3001 ttgttaatga tccaaaaaca gaattatatg ttgctagtaa agatgaacta gaagctagag
     3061 ggtatgatgt gtatgcattt aatatcaccg atccactgca atcaatgagt gataatccat
     3121 tggcactcat cgtgaaatac tggaagcgag gagacataga cacagcgaca cagttaacca
     3181 atactttcac gaatacgatt tattatgatg ccaacgcttc agacaataaa tactttaacg
     3241 aaaatgcgca aaaagctgtc aatgcgctta tttttgcgtt attggaacaa gcggatcaga
     3301 caggggatta ctccaaatta acaccgaata atctcgtcga acttctttct gagattggtg
     3361 gttttaatta ccaagatcct gataatgaat tcaagcagct caatgcttta gatgaattta
     3421 tgaatcagtt accacctgga aacatagcaa aaaaacaata tggggcaaca aaaattggtt
     3481 ctgataaagc gaaaggcaac attctatcga cagcgatcac aggactaaat ccatttacac
     3541 tgacgaaaat tgcgaaaatg acttctcaat caaccattga ttataagtcc attggttttc
     3601 cgaagtattt ggatctcaaa ctacacgaga gtctgttaaa ccagcgaatt gttgtagagt
     3661 tttatcatca aggaaaaaaa tttcatgagg aacgtgtcaa agtaggctat agaggttttt
     3721 gtgaaattaa ctttgattgt catttagaac agggagatca tgtttgctta aaatacgatc
     3781 atcgaggaaa agaatatgtt gcttggtatg agttttctca aagagtattg aaagacgaag
     3841 agggaaatat cgtttatcga aaaaaacgag gagaaaccca tttgccagaa ttagaaaaag
     3901 aagccgttct aacattagat gaggataaaa gtaacttatt agtagagtct attcggatgc
     3961 actataccga taaaccaatg gctattttta tgctcacacc tgattatgat ccatctaacc
     4021 atatcattgt tagtattttt cttagtcagt tgtataaaga attatctacg caatgtgtta
     4081 agactaaagg agataaatgt caccgtagga ttcattttat tttagatgaa tttgggaata
     4141 tgccaccaat ggataatatg gaaggaatca tgacagtcac agctggtaga aacatgttgt
     4201 gggatttgtt tatccaatcc tatcagcaac tttatgcgaa gtatggggaa caaaacgcag
     4261 cgattatcaa agaaaattgt cagacacata tttatattat gtcgacagac gataatacca
     4321 tagaagaatt ttccaaaaaa gtggggaata agacggttga acaagagaac agtaccatca
     4381 attcattagg tatgaatact catatcaatc gcaatgttga ttcagatcgc atcttaactc
     4441 ctgaaagaat ttctactttt ttagaaggtg aaacgattgt actcgctcct ttaaaacgac
     4501 aagatttgaa aggtcataaa atacggccgt atcctatttt taatcatggg gaaacgaatc
     4561 ttccttatgc ttatcaatat attgaagatt tcaatccatc gtgggatatc aatgatatag
     4621 acatacattg tacacatgcg gacttggatc ttaaggcttt acaaattgat tacgattatt
     4681 ttctacctga tatctatgca cgtcgacttt atcacaatat gcacgatcaa atagaagagg
     4741 aagctaaaga aaacattttt attaaaatcg ttcaatcttt agacaactca acggcacaaa
     4801 agttacttat tcaattttat gaagagaagc aaatagatcg tttgcaagaa taccttgaaa
     4861 aattgcgtca aaatgaagaa attacagagg ttataaggaa acgacttttg ctagaagctg
     4921 aaaaggcggt gaaagaatga tcattggagg aatattttcc ggcattgccg actggtggtc
     4981 aggcttattt ggggatgggg atgcagatcc caaaaccgta acgaaagtct tacaagatta
     5041 tggtaattat ctgcagtatc aagatgtatt cagttggatt tgtaatactt ttttatgggg
//
LOCUS       DAPBAB010000127         5092 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPBAB010000127 DAPBAB010000000
VERSION     DAPBAB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.137"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            161..493
                     /locus_tag="RD444_002827"
     CDS             161..493
                     /locus_tag="RD444_002827"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7550134.1"
                     /translation="MIAVSEATYKAFKTEFVSQYQFETMAHLELECFDYVNWWNHHRI
                     HSSLGYKTPIEVLCGGWRSEPLIMSKNRLPSVRAAYFIQSEHAAKKTDLRRLVVKGNW
                     IWCHKQTL"
     gene            complement(663..1058)
                     /locus_tag="RD444_002828"
     CDS             complement(663..1058)
                     /locus_tag="RD444_002828"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDT7550135.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(1068..1916)
                     /locus_tag="RD444_002829"
     CDS             complement(1068..1916)
                     /locus_tag="RD444_002829"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDT7550136.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1931..2758)
                     /gene="panB"
                     /locus_tag="RD444_002830"
     CDS             complement(1931..2758)
                     /gene="panB"
                     /locus_tag="RD444_002830"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDT7550137.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2770..3630)
                     /locus_tag="RD444_002831"
     CDS             complement(2770..3630)
                     /locus_tag="RD444_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDT7550138.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3868..5037
                     /locus_tag="RD444_002832"
     CDS             3868..5037
                     /locus_tag="RD444_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7550139.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRDRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1644 a          952 c          680 g         1816 t
ORIGIN      
        1 tacggaaaga agttctgcac cttataaaat ttgtcaaaaa aactgttgcc attccaactc
       61 ttccatacgg atcgtggaaa agaatttgat aatgaattga ttgatcttat cttagaaaca
      121 tttggcattc aacgctcctt gagtaagcct ggatgtccat atgattgccg tttcagaagc
      181 gacttacaaa gcttttaaga cggaatttgt gagtcaatat caatttgaaa cgatggcaca
      241 cttagaatta gaatgttttg attatgtcaa ttggtggaat catcatcgga tccatagttc
      301 tcttggttat aaaacaccaa ttgaggtact atgcggaggt tggcgtagcg aacccttgat
      361 aatgagcaag aatcggttac cttctgttcg agcggcttat tttattcaaa gtgagcacgc
      421 cgctaagaaa accgatttga ggaggctcgt tgtcaaggga aactggatat ggtgtcataa
      481 acagacctta taatttttgt ctagaaaagt gttgccattc catgtagaca tcatccataa
      541 aatggacttt gtctacagtc tgtatcttct ctttttgtgt ctactttatt gacatctatc
      601 caattagtaa tcaataaata tttcaataat agtctagcta tatataaata acattacaaa
      661 acctaaagaa ttatatctga taaccttcca tgtttttcat atgatgttac tctttcaact
      721 ttattattat catcgacaaa aacaacctta ggtctatgat ccttaacctc attttcattt
      781 aattcacaat atgccattaa aattatttta tctccagttg atacacatct agctgcagct
      841 ccattcaagc aaatcatccc tgttcctctc tctccagcta ttgtataagt ttcaaatcta
      901 tttccattat taacatcaac tatttgtact ttttcatatt catatattcc tgctgcttct
      961 aataaattca tatcaacagt aatacttcct acataatcta actcagcttg ttcaactgta
     1021 gctctatgta tttttccttt taacattgta actttcatta tagcccccta ttgtaattca
     1081 aatgtaaaat tatctattaa tcttgttttt cctataaata ctgctattgc aactaaaact
     1141 gatttttcaa tataatttac tctttgtaat gataaagaat caacaacttc tacataatca
     1201 atctttgcca aattataagt atttatattt ttacttatta tctcaattat ttttaagctg
     1261 tctctctctc cattatttaa agcttcctta gctaaagtta aagatttatt taaaatagta
     1321 gctgaagatc tttcctctaa acttaagtat gtgtttctag aacttttagc tagtccatcc
     1381 tcttccctta taattggaca ccctacaacg tctatatcaa tattaaggtc cctaaccatt
     1441 cttttaatta cagctaactg ttgtgcatcc ttttctccaa aataagctct atctgctggt
     1501 attatgttaa ataatttact tacaacagtg caaacccctc taaaatgtat tggcctacta
     1561 gctccacata aaccttctgt taatccagtt atattaacaa aagtacttgc attgtcacaa
     1621 tacatctctt caacttcagg attaaatatt gctgttgctc tacctcttgt gcatacttct
     1681 atatctcttt ctaagtctct tggataacta cctaaatctt catttttccc aaactgagtt
     1741 ggatttacaa agatacttac aacaacttta tcattttctt ttgatgcttt tctaattaaa
     1801 ctttcatgac cttcatgtaa atatcccata gtaggaacta atcctacctt taagccttgt
     1861 tttttccact cttttacttg acttctaaca tcatttactg ttttaaatat aaccatattt
     1921 cttgcccctt ttaatataat ttttctaaaa catcatcact tattttaaac ccatgttctg
     1981 gacctggaaa aactccatct tttacttcct ctatatattt tgtaaatgct ttattcatct
     2041 cttctgataa attagcatat ttcttcacaa atttaggggt aaaatcacta tacatcccca
     2101 acatatcttg atatacaaga atttgtccat cacaccctgc acctgctcca ataccaattg
     2161 taggtattga tatctcttta gaaattaact ctgcaagctt agttggaaca cattctaaca
     2221 ctacagcgaa tgctcctgct ttttctacag ctttagcagc tcttattaat tctttagcag
     2281 cttccttgtc ctttccttgt accttaaatc ctccaaatgc attaacagat tgaggtgtta
     2341 aacctatatg cgccataaca ggtatagaag cctttactat agcctctatt ttatcgcata
     2401 cttctatccc tccttctaat ttaacaacct gagctcgtcc ttcttttatt aatcttcctg
     2461 cattaactac tgaatcatat actgaagttt gataagacat aaatggcata tctgcaacaa
     2521 ctaaagtgtt cttagctcct cttgtaactg ctctagtatg atgaatcatg tcttccattg
     2581 ttactgaaag tgtatcttca tgtcctagac ataccatacc aagagaatct cctactaata
     2641 ttccatttat cccagcttca tcaattattt ttgctgttga atagtcataa gctgtaagca
     2701 ttgttaattt ttcatttctt aatttagatt ctttaaatgt aactgctgta tttttcatct
     2761 atttctctcc taactctctc ctaaatactt ttctaattca ctataatctc tattaatatt
     2821 ttttaacata gctatctcta atacattttt tgataatagc atatataaag ctctgtcttc
     2881 atctgataaa acttcacagt gacctttaat tgttgaaata tcacaacgtt caactggccc
     2941 tgtcaaactt tctataattc ctttttcttt tatatttcta atattaaatt caattaatgg
     3001 atataaagct tcaattgcca ttttttcagt aaatccacat tgaattagat atgtaacgct
     3061 tgtattaata agtcctagca caaggttact agatactact gatgcggcat gataaagaga
     3121 tttattctcc ttagatacaa ttacagtact attacctaac tttttgaaga aatcacataa
     3181 aaactttgca tatttttcat cgccttcaat tgttataaaa gattttttta attttttgta
     3241 tgaattatat ttatctgata ttgaaaacat aggatggaca gaatagccat aagcaccata
     3301 gtcctgtata tttgaaaaga tatctgaaga tattgcacca ctgcaatggc agattaattt
     3361 gtttttgatt tgataatttt taatttcttg ccatacctcc tgaatttgac catcaggagt
     3421 cgtaatgaaa atagtatcat tttcatctac taattctctt aagtttaaat attgtttact
     3481 agatgtaaaa ttcgaagctg ctaatgacga gtcttcgctt ttactataaa atcctgttac
     3541 ttgtatttta tgttcctgga aatacttccc gaatgaacaa cctaccttgc cggctccaat
     3601 aaaaccaacg ttcataatta tcacctccat aaagaagtga gactacaagt cccattctta
     3661 cgatatgagc ttaaaaataa aaattaaatc aaatatacag tatggttttt tcaaatatca
     3721 tccatagtta atttatcata atatcccata ttttaaaata aaagatagcg taaaatattt
     3781 tgtgtaaata gaaatttagg gtagaaaaaa aagtccattc tgtaaaatta aagttaccac
     3841 acagaaattt tataggagga ctttctcatg actaaattta ctatagaaat tatgcaaaca
     3901 ctactcaata aaggggattt agatgaatta tttcaggatc atttagagcg agcgattaat
     3961 tcacttctac aagcagagct aactgctttt ttagattacg aaaaatatga tcgaaatgga
     4021 ttcaattcag gaaattcccg taacgggaat tattcacgta catttaaaac agaatatggc
     4081 gagttgaatt taactattcc tagagatcgg aatggcgaat tttctcaaca aacactacca
     4141 gcatataagc gaaccaatga ttcattagaa acgaccatta tccaattatt tcaaaaaggc
     4201 attactatgg ctgaaatctc caaattaatt gaaaaaatgt atggtcatca ctatacgcca
     4261 caaacaatct ccaacatgag taaattggtg gctgaagatg ttttagcttt taaagaaaga
     4321 acgttagaag ccaattattc cgttatattt atggatgcga cgcatatccc tgtgaagcga
     4381 cagactgttt cgaaggaagc agtgtatatt acgattggta ttcgtttgga tggaaccaaa
     4441 gaagttcttg ggtttactat tgccccaact gagtctgctt atatttggaa agaagttctt
     4501 caagatctta gaaaacgtgg gttagaagaa gttttattag tagtgacaga cggattaagc
     4561 tgtattgaag aaagtatcca tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg
     4621 catgtatcta gaaatatcgc tcataaagtt cgtgttcgag atcgaaaaga aatttgtgag
     4681 gatttcaaat tggtttacca agcgaattca aaagaagagg cattggatca catcgacttt
     4741 atgactagga aatggaaaaa gcagtatcca agagtcgtca atttactctt gaatcctgcc
     4801 ctattaacct tttataattt ccctcacgcc atcagacgaa caatttattc gacgaacctg
     4861 attgaaggct ttaataagca gctaaaacga tatactcgaa gaaaagaaca attccctaat
     4921 gaagaatctc tagagagatt cttcatttct caatttaatc aatataacca aaaattttta
     4981 ggtagaattc acaagggatt taaagaaatt caggatacat tagagtcgat gatttaactg
     5041 taattaacag aatggatttt ccatttacac ataattcttg acgctaccat tt
//
LOCUS       DAPBAB010000128         4931 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPBAB010000128 DAPBAB010000000
VERSION     DAPBAB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.138"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            85..972
                     /locus_tag="RD444_002833"
     CDS             85..972
                     /locus_tag="RD444_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDT7550140.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="RD444_002834"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="RD444_002834"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDT7550141.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="RD444_002835"
     CDS             2274..2720
                     /locus_tag="RD444_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7550142.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="RD444_002836"
     CDS             2993..4345
                     /locus_tag="RD444_002836"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDT7550143.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="RD444_002837"
     CDS             4516..4803
                     /locus_tag="RD444_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550144.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAPBAB010000129         4818 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPBAB010000129 DAPBAB010000000
VERSION     DAPBAB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.139"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..347
                     /locus_tag="RD444_002838"
     CDS             <1..347
                     /locus_tag="RD444_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7550145.1"
                     /translation="NQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGT
                     PSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFL
                     SYVSIDDLSNLI"
     gene            589..906
                     /locus_tag="RD444_002839"
     CDS             589..906
                     /locus_tag="RD444_002839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550146.1"
                     /translation="MVFEDQYLQIKNSENIEACIEISNSQESNIFVSPENGPVKPNFN
                     YLTYDRFSQNTVFDGYKLEQSSPAIHSGKKVIDKNGYNLGTDFFGIKLDGILDIGAVK
                     SSK"
     gene            928..1740
                     /locus_tag="RD444_002840"
     CDS             928..1740
                     /locus_tag="RD444_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7550147.1"
                     /translation="MNDISFFQIFSLICLAILGGIDPYLSGLQLSKPAISGFLAGIIM
                     GDINTGLLVGATLQLMVLGVGTFGGSSIPDFGTGAIIGTALGVVSGKGIEFAIGISVP
                     VGLLLVQLDILARFCGVYFLHRVDKHIEEENFHKISLEAWLSLVPIALSRAIPVGLCL
                     IFGNEVVNTVLNYAPDWLMGGLKLAGAILPVVGIAILLHYLPIRNFFGFLIIGYILAA
                     YLNIPMMGISFAGLAAAIIHYKKLQDKRLLESKLDTINHIEVVNGEVDDDEI"
     gene            1727..2542
                     /locus_tag="RD444_002841"
     CDS             1727..2542
                     /locus_tag="RD444_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7550148.1"
                     /translation="MMKSNVSKLSSKDLRKVSLRYMFGAQLGWNYERMMNVAYVRAVY
                     PALKKLYKDEKELKSVLQSEMQFYNTSPFLSAFIIGIDLALQVEDGSKSKDAVAAIKT
                     SMMGPFAAVGDSLFGAVIPTIFGSLAAYMGLEGNPLGVLLWLLVSLIILFLRYFELPI
                     AFREGKKLVSSVGGLLKDLTESATLLGVVVIGGLVPSVVKVGVPFKLVIGDKKLSFQT
                     EMLDPIMPAIVPVVLVLLAYWLLSKKSLNSTKVIWIFLVLSILMYSLNILAVL"
     gene            2861..4318
                     /locus_tag="RD444_002842"
     CDS             2861..4318
                     /locus_tag="RD444_002842"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HDT7550149.1"
                     /translation="MKKNQPNVVLIVVDQMRADALSLNSQDKIISTPTLDMMASQGYN
                     FENCYSPVPSCVPARAALLTGLDQETSGRVGYEDEVPWNFKNTLPEVFKEQGYQTECI
                     GKMHVYPSRKRLGFDHVLLHDGYLHVDRKYDKSYGEQFEYSSDYLMFLKESLGSDADL
                     IDDGLNCNSWEARPWMYPEKFHPTNWVVSEGINFLRRKDPTVPFFLKLSFEKPHAPLN
                     PPKYYFDMYMDRLPDTLDLHIGNWEKLEHVVPDVCALRGKLKEDDQRRMLAGYYGLIS
                     HIDHQINRFLMALKEFRHDKDTIIWFISDHGDQLGEHYLFRKGYPYQGSIRIPSFIYD
                     PGDLISAKKHGIKELVKIQDIFPSLVDLVLGQYVNTDGKSVKQLLFGNCEGWRREIHG
                     EHSLGLDSSQYILTEKWKFIWFPVKNTYQLFDMINDPNEMKNLYYDKKYESIIYEMKH
                     KLVGYLKGREEGFVKNGQLIQIGISNIVSTLKSKK"
     gene            4509..4586
                     /locus_tag="RD444_002843"
                     /pseudo
     CDS             4509..4586
                     /locus_tag="RD444_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1663 a          581 c          928 g         1646 t
ORIGIN      
        1 taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact tttgattgtg
       61 gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc atttatttcg
      121 ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga ttgttacgta
      181 gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt attcaatctg
      241 tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca cctttggaag
      301 tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa ttaagaatat
      361 aaaaaaatcc aaatatattt tattgcaact tgctgtttga tcagtttatc tatgttaaca
      421 aaagtcgctc atgctgaaag taaccccatt ggtaattgtt actatgttag ttcgcaaaga
      481 gggagtgaca ctaattcagg attatctata tctgagtctt ttaaaagttt aaataaaata
      541 aatgaactta aataaaatcc aggggataaa gttttattag aaaatggatt ggtttttgaa
      601 gatcagtatt tacaaataaa aaattcagaa aatatagagg cgtgtattga aatcagtaat
      661 agccaagagt cgaatatatt tgtttctcct gaaaatggtc ccgtaaaacc taattttaac
      721 tatttgacat atgatcgatt cagtcagaat acagtgtttg atggatataa attggaacaa
      781 tcatctcctg ctattcatag tgggaagaag gttatcgata aaaatggtta taatttagga
      841 actgattttt ttggtattaa gttagatggc atactagata ttggggcagt gaaaagttca
      901 aaataatata aaggagttag tttgatcatg aatgatattt ctttttttca gatattttct
      961 ttaatatgtt tagctatact aggaggtata gatccttatc tgtcaggatt acaattaagt
     1021 aaaccggcaa tttctggttt tctagcagga attataatgg gagatattaa tacaggctta
     1081 ttagtaggag caacattaca actgatggtc ttaggtgtag gaacatttgg tggctcatca
     1141 attcctgatt ttggcacagg ggcaatcatt ggtacagcat taggtgttgt tagtggaaaa
     1201 ggaattgaat ttgcaatagg tatttctgtt cctgtaggct tattattagt tcaattagat
     1261 attttagctc gtttttgtgg ggtttatttt ttacatcgtg tggataaaca tatagaagaa
     1321 gaaaattttc ataagattag tttagaagca tggttaagtt tagttcctat tgcattatct
     1381 cgtgcaatac cagtaggttt atgtttaatt tttggtaatg aagtagtaaa tacagtatta
     1441 aattatgcac cggattggtt aatgggagga ttaaaattgg caggagctat attaccagtt
     1501 gttgggattg ccattttact acattatttg ccaattagga attttttcgg ttttttaatt
     1561 attgggtata ttttagccgc ttatttaaat attccaatga tgggtatttc ttttgcagga
     1621 ttggcagctg ctattattca ctataaaaaa ttacaagata aacgtttatt agaatcaaaa
     1681 ctagatacta tcaatcatat agaagtagtt aacggagagg ttgatgatga tgaaatctaa
     1741 tgttagcaaa ttaagtagca aagatctgag aaaagttagt ttaagatata tgtttggagc
     1801 tcaactaggt tggaactacg aacgaatgat gaatgtggca tatgttcgag ctgtatatcc
     1861 agctttgaaa aagttataca aagatgaaaa agaattaaaa agtgtgttgc aatcagaaat
     1921 gcaattctat aataccagtc cttttttaag tgcatttatt attggaattg atttagcatt
     1981 gcaagtagaa gatggaagca aatcgaaaga tgctgttgca gcaattaaaa cgagtatgat
     2041 gggacctttt gcagctgtag gcgattcttt atttggggca gtgattccaa caatttttgg
     2101 atctctagca gcttatatgg gattagaagg aaatccatta ggtgtactac tttggttatt
     2161 agtatctctc attatactat ttttgagata ttttgagtta ccaattgctt tccgagaagg
     2221 taaaaaactg gtatccagtg taggtggatt gttgaaggat ttaactgaat cagcaacttt
     2281 acttggcgta gtagtaatag gaggcttggt tcctagtgtt gtaaaggttg gtgttccatt
     2341 caagttagtt attggtgata agaaactatc tttccaaaca gaaatgttag accctattat
     2401 gccagctata gtacctgttg ttttagtttt attggcatat tggttattaa gcaaaaaatc
     2461 tctcaattca accaaagtta tttggatatt tttagttttg tcaatactaa tgtattcatt
     2521 aaatatttta gcagttttgt aatagatgtg ggggcgtgat caactaattt atggcatgtc
     2581 ccctgtttaa ttttctaaat attatatttt taaatgaatg gaaagaagat attagagatt
     2641 taatgaataa taagttatat agataaaaaa ttaaagatat tttgatatca aggtttattt
     2701 tagtataaag tattcgtgac taatcaatca tacgctataa agtttggtaa tccatttata
     2761 tttcttgtta tcacaaaaaa tatattcttt caaaaggaaa tatcaatttc ttaaatcaag
     2821 agagattatt tggatatttt gaatgattgg agtttttaat atgaaaaaga atcaacccaa
     2881 tgttgtttta attgtagtag atcagatgag agcagatgcg ttatctttga atagccaaga
     2941 taaaatcatt agtacgccta cattagatat gatggctagt caaggatata attttgagaa
     3001 ttgttattcg cctgttccaa gttgtgttcc agcaagagca gcgttattaa caggattaga
     3061 tcaagaaact tcgggaagag tgggttatga agatgaggtt ccatggaatt ttaaaaatac
     3121 tcttccagaa gtttttaaag aacagggata tcaaactgag tgtattggga aaatgcatgt
     3181 gtatccttca agaaaacggt tgggatttga tcatgtacta ttgcatgacg ggtatcttca
     3241 tgttgatcga aaatatgaca aatcttatgg agagcagttt gaatattcta gtgattactt
     3301 aatgtttcta aaagaatcat tgggctcaga tgcagatttg attgatgatg ggttaaattg
     3361 taattcatgg gaagcacgac catggatgta tcctgaaaaa tttcatccaa caaattgggt
     3421 ggtttcagaa ggaataaact ttttaaggcg gaaagatcct acagtaccat ttttcttaaa
     3481 attatcattt gagaaacctc atgctccttt gaatccacca aaatattatt ttgatatgta
     3541 tatggataga ttaccagaca ctctagattt gcatattggt aattgggaga aattagaaca
     3601 tgttgttcca gatgtttgtg ccttaagagg aaaattaaaa gaggatgatc aaagaagaat
     3661 gcttgctgga tactatggcc ttatttctca tatagatcat cagatcaatc gctttttaat
     3721 ggctttaaaa gaatttagac atgataaaga tacaatcatt tggttcattt cagatcatgg
     3781 agatcaatta ggagaacatt acttattcag aaaagggtat ccttaccaag ggagtattcg
     3841 tatcccgtcg tttatttacg atcctggtga tctgatctca gcaaaaaaac atggaattaa
     3901 agagcttgta aaaatacagg atatatttcc ttcattagtt gatttagtat taggacaata
     3961 tgtaaataca gatggaaaaa gtgttaaaca gttgctcttt ggaaactgtg aaggatggcg
     4021 gcgagaaatt catggagaac atagtttagg gctggattct agtcaatata tattaacgga
     4081 gaaatggaaa tttatatggt ttcccgtaaa aaatacgtat caattatttg atatgataaa
     4141 tgatccaaat gagatgaaaa acctatatta tgataaaaaa tacgaatcta ttatttatga
     4201 aatgaaacat aaattagtag gatatttaaa aggtagagaa gaggggtttg tcaaaaacgg
     4261 acagttaatt caaataggaa tttcaaatat cgtttcaact ttaaaaagta aaaaataaac
     4321 taaaagtgaa gatgcaataa aaaatcaaca tagggtatac tgagtcaaaa tatactcaga
     4381 tacaggcaaa actttgcgtc tttatttatt tcaaaaaaat caaatattcg gatggtaaca
     4441 aacatactac tttgtttggg ataaaatcag atctgagtca gagtaagttc attctgattc
     4501 agttttttat gtctaacgac gataaaagtt tagcacatac aagatggaat tgtaagtatc
     4561 ccatagtctt taccccggag tatcgatgaa aagtgattta tggaaaatta agagtaagta
     4621 actgttggtg atagtatcac cccaaattag tatttccagt tttatgagct atttattaaa
     4681 tgattaaaaa gagatgaggg tgtgagctgt tactgatact ttaaaaaaaa cttttaaagt
     4741 actatcaagt gattagctct tctagagagg ttaaaaagaa tttgttttct agagtagata
     4801 ttacttgaga gtgtaaaa
//
LOCUS       DAPBAB010000130         4789 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPBAB010000130 DAPBAB010000000
VERSION     DAPBAB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.140"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(69..1166)
                     /locus_tag="RD444_002844"
                     /pseudo
     CDS             complement(69..1166)
                     /locus_tag="RD444_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1296..2672)
                     /locus_tag="RD444_002845"
     CDS             complement(1296..2672)
                     /locus_tag="RD444_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7550150.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2672..3328)
                     /locus_tag="RD444_002846"
     CDS             complement(2672..3328)
                     /locus_tag="RD444_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7550151.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3338..4615)
                     /locus_tag="RD444_002847"
     CDS             complement(3338..4615)
                     /locus_tag="RD444_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7550152.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4634..>4789)
                     /locus_tag="RD444_002848"
     CDS             complement(4634..>4789)
                     /locus_tag="RD444_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDT7550153.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1502 a          870 c          578 g         1839 t
ORIGIN      
        1 ttcataaatc tattataaaa gaaaagactg tagacatcat ccataaaatg gactttgtct
       61 acagtctgcg cattctcatt agagaacgct cctttttttg tgacttttct gaagctggag
      121 tggacacttt caaccgcatt ggtagtgtac ataatttttc gaatggcact accataatca
      181 aatagttgtt caacatgaga aaagtttcgt ttgcatacat ctactgcacc agaataatga
      241 gaccaccgat tttgaaaact gtcgaaggca gcgtgtgcag cgtttaaaga agaagcactg
      301 tagaattttt tcatgtctcg acagacttcc ttataatcct tacttgggag gtatcgtaac
      361 gcatttcgaa caagatgaac aatacagcgc tgcacaatga cagaaggaaa aattgctttt
      421 gcgccttctt tgagaccgga aacaccgtcc atcgaaatga aaaaaacatc ttctacacca
      481 cgtactttca gttcatcaaa tacttgcatc cagcgatttt tagattctgt ttgattgagc
      541 cataggccta aaatctcctt gtttccttta agatcatagc caagaatggt gtacacagca
      601 tattctttag cttcatagtt ttcgcgtaaa gtgacgtaca tacaatcaac gaatagaaat
      661 gcataacact tggctaatgg ccgagcttgc cattcttcca attcaggaag gacggcatcc
      721 gtgatatcag aaatcatttc gtgggaaata tcaaagccgt aaatatcttc aacggttgcg
      781 gcgatatcgc attgactcat tcctcgtgca tacatggaaa gaaccttacc ttcgatgtcg
      841 gagacatctc gttttctatt aggaatcaac tcaggttcaa aagaagcctc ccggtctctg
      901 ggaacatcaa tgtttacttt accaaagctc gttttaagcg tcttattgcc ataaccgttt
      961 cgtcgattgt catgtttctt tggctcttta gaatgggcgt cataccctaa atggttattc
     1021 aattctcctt gaagcatttt ttcaaataag ggaccaaaca catcttttaa aacatcttgc
     1081 atatcctcta cagattcggg ttgataagta ttcaaaatgg attcagctaa cttctcagca
     1141 tcaggatttc ttttctttct agccatcgtg taatcacctc ttgatcttat tttagaaaaa
     1201 taaaaaccgt gaagcaacca cccgcctagg attgatggtt acttacacgg tttatattac
     1261 actctcattt ttggtgctaa aaagcaccga aaattttaat tattgtcaat taataattct
     1321 tttggattcc ttcttaatat attgaatgaa gaaataatca atgatacaag aagtactaaa
     1381 ctcataaata taactacata aatcatgaat ttaggtaata cattgatatc taaacttgat
     1441 aatgtcttgt taaatccttc agcttctgcc ccaccaccta attggctaga tgcagattgt
     1501 ttagctattt gtttagctat atcaccagta accttattca atatattatt cccaagcttg
     1561 tcagctgtat attgagctaa gaaataggaa ccaacgaatg cggggattga tataaatacc
     1621 atctcaatta ggaattgacc aaaaatttct aattttgata tacctaatga tagtaatact
     1681 gctatttctt tctttctagc attcatccat aagaataata ataatgaaac tacaactcct
     1741 gcaaatatta atgagccaaa aaataattta tttgaaattg agtaaatacc tgatatagat
     1801 tgttgtaatg cagggtaatt tgatgaactt ttaatcaaat tatattctct ccaatttata
     1861 tctaatttcc caagatcttt tattacacta tcaagattct tatcaccttt tacaaagaat
     1921 gttgcatctt ggtatacagc tgtatcttcg gtattaccat aaactttagc agctgtatga
     1981 atatcagtga ttaatgtatt ttcgtatagt tcttgtgctg cacttactcc accgctatta
     2041 tgcccatcga atagaccttt aatttctact tctactgttt catctgcacc tttctcatta
     2101 tcagcatcaa ataaattaga ttttattttt atcttatctc caactttaag gttatttttt
     2161 tcagctaaat ctttatgcat taagatttta tttttatctt cattttctaa atgctttcct
     2221 tctactaatt tatatgcttc tgatacaaat tttgtttctt ttgttgagtc gttaacccca
     2281 gttaacataa ctgttctttt aaaattcttt gctctttcag gtgattgatt cgcaagagtc
     2341 tcttgagttt caataatatc ataatcaact aaatctgcaa cactgtttat tctttttaca
     2401 taggagtcta tactatctgt ttgagatatc tttttaatat cttcaccttt tacatttccc
     2461 ccacctctag gtgttcctgg atttacttgt ctatttatct ccatagaaaa actatttgtt
     2521 atattagcaa atgtttcttt tgaagctctg tccgtagcat ctttaataga taaactaatg
     2581 agacttaaag ttgacataga taaaataact aatatgataa ttaatgattt taaacttttt
     2641 ctagttacat aagcaaatgc attttttatc attattcaac ctccaaattc attttattta
     2701 cttttttcaa ctttttacca cttagttcta aaatgatatc cgcagaatct gctacttcct
     2761 tactatgtgt tacaactatt acacatttat ttctatcttt agctaatgtc tttaatatat
     2821 taattatttc tccagcagta acactgtcta gattaccagt aggctcatca gctagtatta
     2881 ttggagcatc tgataccaat gctctagcaa tagctactct ttgttgctgt ccaccagata
     2941 atttcataac atttcttttt atttgttttt tatctaaacc tagttcaaac aagatacttt
     3001 catctgctga tttatttact aatctaatat tttcaattgg tgataaataa tctattaaat
     3061 tatagttttg aaatactaaa gatatattat tttttctatg attactatat cctttatttt
     3121 gtatatcttc attcttaaac aatatttttc ctgtttgagg tttatcaagt ccagcaagta
     3181 aggaaagaag tgtagatttt cctgttcctg actttcctac tatcgcataa aacttcccaa
     3241 gttcaaattt ttgatttact cctgacaaaa ctttttcttt agaatttgca taactatatg
     3301 ctacattctt tatttctaat atatccatta ttttctccta acttattttt gataagattt
     3361 ctttaggctt cttgattaac attaatgaag aagcaaatac aactgataaa acaataatac
     3421 ttattaatat taaataactt tgtccaagtg ttgttatatt tgacataaaa ctactattat
     3481 ttattaaact tccaccggaa atcattgaat cctctgagtt aatgaatcca tctacaatta
     3541 cttttagtaa tacatttcct aaaaataagg aagatattat acttggtatc gatatgaata
     3601 ttaactcgaa tataaattgc attataattt gtatcttaga tgttccaata gataagaata
     3661 tacctatttc ataaattctt tcccttaacc atagaatcaa gattaatgaa agaacaacca
     3721 tcccacctaa cataatagaa taagtcatta cttttattat atattttatt ccactcactg
     3781 actctaaaga ctcttcaaac gcattagaat ctttttcaac aaaatacttt gactcatcaa
     3841 ttttaagctc tttcaatttg tttaaggcta agtctgtaga ttctgcacta ccagaataca
     3901 ttaaaatttt atttgcaatt tcattatttt ctgaattgtt taatatctct tggcttgttg
     3961 aataatctac aaacaccata ttttcactaa aatcagaaga taatcctgta tatgtttcct
     4021 gttttttacc agaaaagatc cctataattt taaatttatg actttttatt tttccacttt
     4081 tttcaatatc tagtaattca agatcaactt catcacctaa ttttagattg ttttgtttag
     4141 caaattcttc atgaacaata attgaattct tatcattttc ttctatattt ttaccttctt
     4201 taattgtaaa tactccacta ctaaataaaa tatttctttt agtattattt gtagcttcga
     4261 gtgaaacaac attcttaaat tcgtcagata aatcttctct atttattctt tgttctccgc
     4321 taactacttt agcatctttt agttttgcta atccatcata ttgaattatt ttttcttcaa
     4381 tttcttttaa tttttcaata tctttaaatt gattaacatt aaaatattta ccatcttttt
     4441 ttgttattga tattgaagaa ttagaacttt catataaagc cttttctatt tcattgcttg
     4501 atttcattat tgttaaacaa gaatacaagc aagaaagaac tatagttaat atgataaaaa
     4561 ttattaaagt tctatttttc tttcttgtaa tatatgctat agcatttttt atcactttcc
     4621 taactactcc tctctatttt cttactccat aactaattgc atccactgga caagcatttt
     4681 tacatcgtcc acatctgata cactccagat gattgcaatt ctcaactgaa tctttattca
     4741 tttggcaaac ttttttacat tttccacaat taatacactt atccttatc
//
LOCUS       DAPBAB010000131         4728 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPBAB010000131 DAPBAB010000000
VERSION     DAPBAB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.141"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(52..522)
                     /locus_tag="RD444_002849"
     CDS             complement(52..522)
                     /locus_tag="RD444_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDT7550154.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKITAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="RD444_002850"
     CDS             complement(681..1139)
                     /locus_tag="RD444_002850"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDT7550155.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="RD444_002851"
     CDS             complement(1310..2455)
                     /locus_tag="RD444_002851"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDT7550156.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPHHELHGVAKQQMSGFSGMISFTLKNEETAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="RD444_002852"
     CDS             complement(2468..3385)
                     /locus_tag="RD444_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDT7550157.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="RD444_002853"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="RD444_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDT7550158.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="RD444_002854"
     CDS             4477..4647
                     /locus_tag="RD444_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550159.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEREETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1477 a          854 c          889 g         1508 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtta ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcagt ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 ggggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct cgaatattca tattgcttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatatggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggattgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaattcttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 aaccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga gagaagaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgtttttg tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattctta
//
LOCUS       DAPBAB010000132         4593 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPBAB010000132 DAPBAB010000000
VERSION     DAPBAB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.142"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            104..355
                     /locus_tag="RD444_002855"
                     /pseudo
     CDS             104..355
                     /locus_tag="RD444_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            448..1242
                     /locus_tag="RD444_002856"
                     /note="aph(3')-IIIa"
     CDS             448..1242
                     /locus_tag="RD444_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDT7550160.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1652..1735
                     /locus_tag="RD444_002857"
                     /pseudo
     CDS             1652..1735
                     /locus_tag="RD444_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1784..1867
                     /locus_tag="RD444_002858"
     CDS             1784..1867
                     /locus_tag="RD444_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDT7550161.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1992..2729
                     /gene="erm(B)"
                     /locus_tag="RD444_002859"
     CDS             1992..2729
                     /gene="erm(B)"
                     /locus_tag="RD444_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDT7550162.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2992..3855)
                     /locus_tag="RD444_002860"
     CDS             complement(2992..3855)
                     /locus_tag="RD444_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDT7550163.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3857..4129)
                     /locus_tag="RD444_002861"
     CDS             complement(3857..4129)
                     /locus_tag="RD444_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7550164.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4146..4355)
                     /locus_tag="RD444_002862"
     CDS             complement(4146..4355)
                     /locus_tag="RD444_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDT7550165.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4453..>4593)
                     /locus_tag="RD444_002863"
     CDS             complement(4453..>4593)
                     /locus_tag="RD444_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HDT7550166.1"
                     /translation="RSNKVSTWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENE
                     KE"
BASE COUNT         1412 a          775 c          847 g         1559 t
ORIGIN      
        1 cttgggagtt ttgagactac tatatgcgtt tttttgttga tttaccaaca tgtttagaca
       61 ttttcctcta ggaaaaaatt aactagcacc acgggttatc ttttatatag aagatatcgc
      121 cgtatgtaag gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga
      181 atgggcaaag cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat
      241 agcttgtaaa ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata
      301 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
      361 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
      421 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
      481 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
      541 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
      601 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
      661 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
      721 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
      781 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
      841 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
      901 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
      961 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     1021 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     1081 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
     1141 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     1201 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga tttagatgtc
     1261 taaaaagctt taactacaag ctttttagac atctaatctt ttctgaagta catccgcaac
     1321 tgtccatact ctgatgtttt atatcttttc taaaagttcg ctagataggg gtcccgagcg
     1381 cctacgagga atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca
     1441 tttaacttga cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt
     1501 ttaattataa aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg
     1561 tgtatttctt atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg
     1621 agagtgattg gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa
     1681 acttaagagt gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta
     1741 aattagatgc taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat
     1801 gcgttatgta gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga
     1861 taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat
     1921 aactaaaatt acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca
     1981 ggagtgataa catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag
     2041 tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg
     2101 gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta
     2161 ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg
     2221 tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa
     2281 ttgttgggaa tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa
     2341 gccgtgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata
     2401 ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc
     2461 tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc
     2521 gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat
     2581 gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac
     2641 acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata
     2701 gttatctatt atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag
     2761 ttacacgtta ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta
     2821 ccaattaatt cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc
     2881 ttatcgatac aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa
     2941 aaaaagagta gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc
     3001 tggaagttta ggtgttttgg gtattggtgg tgtaggtggc tgcaagcttt ccaatttttg
     3061 ttgaattgct ttaaattcag gtgtctcttg gtgtttattt agaaccattt tttgctctat
     3121 tcgttctaaa gtcggttgaa tttctttccc tgatacttta cgattcaatt ctttttctaa
     3181 ggtttctttt ggactaatag aaggtgtttc caaacttgaa tagagtttta ctccttctct
     3241 gttatatagc cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg
     3301 taagtttttg acaacaatat catgcgcttg ttttggtgtt gccctggctg tcattggatc
     3361 atctgcatac atggtttcat atcgttcaat tgttcctaaa tatgagttga ttttaggtac
     3421 tgccatgaca tacatttttg tttcataacc tttggcttga agcattgttg cggtttgaat
     3481 aggaacgtct gttgttcgtc ctgtaccttc gatcaccaaa ttataccctt gatcgctcaa
     3541 acggcttatg atcgcttctg tcatgcgatt agaataaggg gtaacgtgtt ttactacgtc
     3601 tttttcataa agtttcacta gttcatcaaa attagggtgc tgttgtttaa aggtatcatt
     3661 atcaatgaca ataacattcc cttgtgtttc ttcaaaaatt gctgatcgca aactggtttt
     3721 ccctgaccct ggttgcccac caagtaaaaa agcggttggc gattcaaccg ctttttttcc
     3781 ttgaatcaat tcttctaaat tatcatttaa gcgattctca aattgtttgt cagtaaaatt
     3841 gactatattt gccatattaa gccactttct ctttattcaa aacttgtttt gagtaacgat
     3901 tcattgaccg ccaatactca tgaacggctt gtaattcttc taaagaataa tcaaaaagat
     3961 ttacacgttt tgcaagcttt aattggttta ataaattgac ttccaataat tgagagtcat
     4021 ttttatttaa ttcatggttc aaaacatagt ttaatactcg attataaacg cttgccgata
     4081 attcgttaat gatctctatt tcaaatgttt tttcatacgt aactgccatt tcattttcac
     4141 tccttttaaa gtttgtcagg taaatatttc cgtatatatt cttctaaggc ttgatccata
     4201 acttctttta cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtca
     4261 gcacgaacac gaaccgtctt atctcccatt atatcttttt ttgcactgat tggtgtatca
     4321 tttcgttttg ctttttgtgc gcctaaattt cccacaatca ctcacttctt tctatttctt
     4381 cttactctta ttttatcatc aacaatcaca aatcacaagt gatttgtgat taatcacttg
     4441 tgatttgtga ttctattctt tttcgttttc taattgaata attcgctcaa gcatttcaaa
     4501 aaatacgttc ttatacatgg ataaaacttt tttgtcatag cctttatgct ctgtaatgcc
     4561 gtttttagac caggtactta ctttattact tcg
//
LOCUS       DAPBAB010000133         4467 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPBAB010000133 DAPBAB010000000
VERSION     DAPBAB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.143"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(25..570)
                     /locus_tag="RD444_002864"
     CDS             complement(25..570)
                     /locus_tag="RD444_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin ECF transporter"
                     /protein_id="HDT7550167.1"
                     /translation="MLPTSNSRFPIRRITTLALFSAACIVGRILFTWIPNVQPMTAIL
                     LLLAFYGSYTDALIVTLVSLLGTNLYLGMGSWTISQLIAFSSIVTVFHFLSNCALIKH
                     HISIQAIIAFLCGLLYGLIVSRVEVFIYQLPSFWAYYFQGLFFDILHGTGNLIFYLVL
                     VSVFKRILLPAINSSKNKLNS"
     gene            720..3203
                     /locus_tag="RD444_002865"
     CDS             720..3203
                     /locus_tag="RD444_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HDT7550168.1"
                     /translation="MDYPKKYIEKLNQDISSYKEVAKITAEMTQTFDGISRLIMLDRY
                     AFKDMSGKTLTVGDLVILTTHADPQYPARGIGKIEEINKEQLIIRIEEEFQSSLTDPE
                     EIASGRVIRNINEVEKPLELYFEQIAARVARGISDIEGEKKAQFFTEFYQHLTEQNLI
                     PAGRVLYGAGTNSEVTYFNCYVMPFIHDSRGGLAKHRQEVMEIMSRGGGVGTNGSTLR
                     PKSAIVHGVNGKSSGSVSWLNDLANLTNLVEQGGSRRGAQMIMLNDWHPDIFEFIISK
                     IQNPAILQNLIHTSQSPTVRQLASEKLTFEPLTANEKEWLEFLVDHPTNDMEDKFLKT
                     AQQKLKAGGTYSVKDPDFLTGANISVCLTKEFMEAVETDQTYELRFPAIEDYDASEMA
                     DYDQHWMEVGDVREWAETGRKVKIHQTIKARELWDLITYCATYSAEPGIFFIDNANEM
                     TNAQAYGQKVVATNPCGEQPLTPYAVCNLAAINLANMVDKKTKVVDFEKLTDVVRASV
                     RFQDNVIDATPYFFKKNEQQAKGERRVGLGVMGLADLLIYCEKVYGSEDGNRLVDQVF
                     EKIAITAYQTSVELAKEKGSFPFLVGKTVEETTALRQRFIESGYMKKMPESIRQAILK
                     YGIRNSHLLTVAPTGSTGTMAGVSTGLEPYFSFSYFRSGRLGKFIEVNAEIVSEYLEK
                     NPEKTADNLPPFFVTAMELSPKDHVSVQCTIQRWVDSSISKTVNAPKGYQVKQVAAIY
                     EDLYQGGAKGGTVYVDGSRDSQVLTLEADGNVFEEPVVGVADNQITLDSLESSEAEEI
                     CPICHEGRIEEIGGCSTCTNCKVQLKCGL"
     gene            3217..3780
                     /locus_tag="RD444_002866"
     CDS             3217..3780
                     /locus_tag="RD444_002866"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDT7550169.1"
                     /translation="MKIYTKSGDKGNTSIIGGERIKKSDARVCAYGSVDEVNSWVGKI
                     ISELDDKKFELLKKELVVLQILLFDIGTDLATPIGIKEMIVGKEEVTKIETLIDFYQA
                     KVPVIEKFILPGGHPVACDMQITRTVIRRAEREITRLIVSDEPINLHAYKIMNRLSDL
                     FFVLARYVNHVYATKEPFYERAGKVFH"
     gene            3796..4200
                     /locus_tag="RD444_002867"
     CDS             3796..4200
                     /locus_tag="RD444_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDT7550170.1"
                     /translation="MKKLATLLTVFIGLFIMTGCQSNQNDQASSKASTQATSEVAKSS
                     ALIKLTDSDKEITSKTVTYKSGESLLVILKANFEVKERDGFITSIDGHAQDESQNKYW
                     TFTINGKMGEKGAGETTLDNDDQVVFNLGVFK"
     gene            4402..>4467
                     /locus_tag="RD444_002868"
     CDS             4402..>4467
                     /locus_tag="RD444_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7550171.1"
                     /translation="MSNFKGKQFEKDIIMVAVCYYL"
BASE COUNT         1561 a          691 c          941 g         1274 t
ORIGIN      
        1 agatattgtg atttagatac tcatctaact atttaattta tttttcgaac tattaatagc
       61 aggtaataaa attcgtttaa acacagaaac taatacaaga taaaagatta aattacccgt
      121 tccatggagg atatcaaaaa ataatccttg aaaataatag gcccaaaaag aaggtagttg
      181 atatataaag acttcaactc tcgatacgat caatccgtaa agaaggccac ataaaaatgc
      241 aatgattgct tggatagaga tgtgatgctt aatcagggca cagttagata aaaaatgaaa
      301 aaccgtaacg atactactga aggcgatcag ttggctgatt gtccaactac ccattcctaa
      361 ataaaggttc gtgcctaata agcttactaa agtaacgatt aaagcatctg tataacttcc
      421 ataaaaagcg agtaaaagta gaatggctgt cattggttgt acattcggta tccaagtaaa
      481 aaggattcta ccaacaatac aggcggcact aaataaagcg agggtggtaa tccttctgat
      541 tggaaagcgt gaattagagg ttggtaacac taggaaaact cccttgtttt aattgataat
      601 agttctcatt gtatcagaat aaaaattaat tacaagattg aattacactt tatatagggc
      661 tataatcatt ggcaggcaca atatgttgtg tttgttaatg gtaaaggtgg tattatttaa
      721 tggattatcc taaaaaatat atagaaaaat taaatcaaga tatctcttct tataaagaag
      781 tcgctaagat aactgcagaa atgactcaaa catttgatgg gatttcaaga ttaatcatgc
      841 ttgatcgcta tgcatttaaa gatatgagcg gaaaaacttt gacagtcgga gatttggtta
      901 ttttaacaac ccatgctgat ccacaatatc cagcaagagg gatcggaaaa attgaagaaa
      961 tcaataagga acagttgata attcgaattg aggaagaatt tcaaagctcg ttaactgatc
     1021 cagaagaaat tgcttcagga cgagtgattc gaaatataaa tgaggttgag aaaccgcttg
     1081 aactttattt tgaacaaatt gcagctcgtg ttgctagagg aatctctgat attgaaggag
     1141 aaaaaaaagc tcagtttttt accgagtttt atcagcattt gacagagcaa aatctcattc
     1201 ctgctggtcg tgtactttat ggtgcaggaa cgaattcaga agtaacatac tttaattgct
     1261 acgttatgcc attcatccat gactctcgtg ggggcctagc aaaacatcgt caagaagtga
     1321 tggagattat gagtcgcggt ggcggtgtag gaacgaacgg ttcaacgcta cggccaaaat
     1381 cggcgatcgt ccatggggtg aatgggaaat catcaggttc agtttcgtgg ctcaatgact
     1441 tagctaattt gacgaattta gttgaacaag gcgggagtcg tcggggagca caaatgatca
     1501 tgttgaatga ttggcatcca gatatttttg aattcattat ctctaagatt cagaatccgg
     1561 ccattttaca aaatttgatc cacactagcc aaagtccgac agtgcgccaa ttagctagtg
     1621 aaaaactgac ttttgaacca ctgacagcaa atgaaaaaga atggttagaa tttttggttg
     1681 atcatccgac aaatgatatg gaggataaat ttttaaaaac ggctcaacag aaattaaaag
     1741 cgggtggtac gtacagtgtg aaagaccctg atttcttgac cggagccaat atctccgtct
     1801 gtttaacaaa agaatttatg gaagctgtcg aaaccgacca aacttatgag ttacgctttc
     1861 cagcaatcga agactatgac gctagtgaaa tggctgatta tgatcaacat tggatggaag
     1921 ttggagatgt cagagaatgg gctgaaacag ggcgtaaagt aaaaatacat caaacgatca
     1981 aggctcgcga attgtgggat ttgattactt attgtgcaac gtattcagca gaaccgggaa
     2041 tattttttat cgataatgct aatgagatga ccaatgctca agcctacgga caaaaggtag
     2101 ttgcaaccaa tccatgtggg gaacagccat tgacgcctta tgcggtatgc aatctagcag
     2161 caattaattt agccaatatg gttgataaaa aaacgaaagt tgtcgacttt gaaaaattaa
     2221 ctgatgtcgt aagagctagt gttcgttttc aggacaatgt aattgatgcg acgccttatt
     2281 tttttaaaaa aaatgagcag caagctaaag gtgaacgacg cgttggactt ggggtcatgg
     2341 gattggcaga tttattgatt tattgtgaaa aagtctatgg atctgaagat ggaaaccgtt
     2401 tagtagatca agtatttgaa aaaattgcta ttacggctta tcaaacgtcc gtcgaactag
     2461 caaaagaaaa aggaagcttc ccatttttag taggcaaaac agtcgaggaa acaaccgcgc
     2521 ttcgtcaacg ttttattgaa tcgggttata tgaagaagat gccggaatca atccgacaag
     2581 cgattctaaa atacggtatt cgtaattctc atttactaac agtcgcacca acagggtcaa
     2641 cgggtactat ggctggtgtt tcaaccggac tagaaccata tttctctttt tcttatttta
     2701 gaagtggtcg cttaggaaaa ttcattgaag ttaacgctga aatagtcagt gaatatttag
     2761 aaaaaaatcc agagaaaact gcggataatc taccaccatt ctttgtcact gccatggagc
     2821 tttcaccaaa agatcacgtg agtgttcaat gtacgatcca acgttgggta gatagctcta
     2881 tctcaaaaac agtcaatgca cctaagggtt atcaagtgaa acaagtagct gcgatctatg
     2941 aagatctgta tcaaggcggt gctaaaggtg gaactgtcta tgtggatggt agcagggatt
     3001 cgcaagtgtt gacacttgaa gcagatggca atgtatttga agaacctgta gtaggggtgg
     3061 cagataatca aattactctt gactcattag aatcatcaga agcagaagaa atatgtccga
     3121 tttgccatga gggtagaatt gaagaaattg gtggttgcag tacctgcact aactgtaaag
     3181 tacaactaaa atgtggcttg taaaaggagc tattcgatga aaatttatac taaaagcggc
     3241 gataaaggaa atactagtat tattggtgga gaaagaataa aaaaatctga cgctcgcgtt
     3301 tgtgcttacg gtagcgttga tgaagtaaat tcttgggtcg gtaaaattat ttcagagttg
     3361 gatgataaaa agtttgagct gttgaaaaaa gaactagttg tattacaaat tttacttttt
     3421 gatattggaa cagacttggc tacacccatc gggattaaag aaatgattgt agggaaagaa
     3481 gaagtaacta agattgaaac gctgattgat ttttatcaag caaaagttcc agtcattgaa
     3541 aaatttatct tacctggagg acatccggtg gcatgcgata tgcaaattac acgaacagtt
     3601 attcgtcggg cagaacgtga aatcacacga ttgattgttt cagatgaacc tatcaattta
     3661 catgcataca aaattatgaa tcgcttatca gatttatttt ttgtacttgc acgttatgta
     3721 aatcatgttt atgctaccaa agagccattt tatgaacgag cagggaaggt attccattaa
     3781 gagagggttt taaacttgaa aaaattagca acattattaa ccgtttttat cggactattc
     3841 attatgacag ggtgccaatc aaatcaaaat gatcaagcct cttcaaaagc atccactcaa
     3901 gcaacaagcg aggttgctaa atcaagcgcg ctgataaaat tgactgatag tgataaagag
     3961 atcacttcaa aaacagtcac ttacaaatca ggagaatcat tattggtaat tttaaaggct
     4021 aactttgaag taaaagaaag ggatggcttt attacttcaa ttgatggtca tgcgcaagat
     4081 gagagtcaaa ataaatactg gacattcact atcaatggaa aaatgggtga aaaaggcgca
     4141 ggtgaaacta cattagataa tgatgatcag gtagttttca atttgggtgt atttaaataa
     4201 aaattatcgt tctttacagc aattgagcga taattaagta agagaaaatt attagttttt
     4261 aaaggaatat gttaatttta aaacacgttc aaaccaatca tttcgaaggg gttgaacgtg
     4321 ttttttggtt ctgttgcaaa gttttatcta aatacctatt tagtgtaaaa tagctgaaac
     4381 taaaacataa ggagaattac catgtccaat tttaaaggta aacaatttga aaaggatatt
     4441 atcatggtag ccgtatgcta ctattta
//
LOCUS       DAPBAB010000134         4156 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPBAB010000134 DAPBAB010000000
VERSION     DAPBAB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.144"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            178..774
                     /locus_tag="RD444_002869"
     CDS             178..774
                     /locus_tag="RD444_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7550172.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="RD444_002870"
     CDS             898..2697
                     /locus_tag="RD444_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDT7550173.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="RD444_002871"
     CDS             complement(2975..3187)
                     /locus_tag="RD444_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550174.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="RD444_002872"
     CDS             3649..3798
                     /locus_tag="RD444_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550175.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4051..>4156)
                     /locus_tag="RD444_002873"
     CDS             complement(4051..>4156)
                     /locus_tag="RD444_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7550176.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1493 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctataatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcg
//
LOCUS       DAPBAB010000135         4119 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPBAB010000135 DAPBAB010000000
VERSION     DAPBAB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.145"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            870..1910
                     /locus_tag="RD444_002874"
     CDS             870..1910
                     /locus_tag="RD444_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDT7550177.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPITAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2734..3066
                     /locus_tag="RD444_002875"
     CDS             2734..3066
                     /locus_tag="RD444_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDT7550178.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3685..4026
                     /locus_tag="RD444_002876"
     CDS             3685..4026
                     /locus_tag="RD444_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDT7550179.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1512 a          522 c          725 g         1360 t
ORIGIN      
        1 ctatttacac aaaatatttt acactctcca agcatgtagt tttatctgtt aattatgtac
       61 tacctgaata aagtagttta taattaacaa gtatatatat ttaaaaattt aagattaatt
      121 tttttatgac ttgtacaaat ttatctgata ttctcttatt aagaactacc taatttgggt
      181 agttcttatt ttttacattt taagtagtat ttaattgcaa tttggtagta tttttttatt
      241 atcttattct tagaacggta gtacttaata taaatatagg tagtatctaa tttaagtatt
      301 aatggtagta tctaaattaa caggtagtat gtaactactt taaagtaatt tttttcagat
      361 taacacagta gtatcttatt aaataagtta attttaatgg tagtacataa ttattatata
      421 tatctgaaat tggtagtact tattgtattt tttagtagta tataatctat tgttttctag
      481 cctaaagcct tgtcttataa gaataatata atatatacta ctgaaataag gtagtatata
      541 ttactagtaa tggtagtatg ttttatatgc ataaggtagt tcttaattta ttttttgttt
      601 tgacactggt agtacttaat tatctttaat tttaaaacta aattttattc atacgttttt
      661 ctggtagtat ttaattataa ataatattta tttaaagtag ttcttaatga ctttaaaggt
      721 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
      781 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      841 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata
      901 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      961 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
     1021 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
     1081 aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
     1141 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
     1201 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
     1261 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
     1321 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
     1381 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
     1441 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
     1501 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttgtccctg
     1561 aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg
     1621 tccagactat tcataatgca aagaaaaaag ctgaaataga aagtggtatt tcgatagttt
     1681 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1741 aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct
     1801 atgtaaaaaa ttggtttatc gaaaaaccaa taactgctaa agtatcaagt gaaaaacgtt
     1861 tgaattatga aagctcccca agcactatta cgaaagactg gttagagtga aactttactg
     1921 acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattctaat
     1981 tatattacga atattataaa ataatagcta ttttaataga atttataagt taaaatatta
     2041 tgataaaaat cacaaacgta gccttttacg tcataagtgt taaaagaaat aattaatttc
     2101 aaatatagaa aagggaacgt atgtttgcat actccttttt tttgagttat aataatacgc
     2161 gaagagagat aatttcatta tttaaacaag ctaaaaatct tgatattact tcttttcagg
     2221 caatacgaat attattcgct tacatttttt gtataaattt aattgtctgc tatgcttgtg
     2281 atgaattgat tttcgttctg cacttttgga tgataaattt ggacaattga aaatacaaaa
     2341 aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc atacacggca
     2401 ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc gcaatggatt
     2461 tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag gttaaaaata
     2521 cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc gtgtggctct
     2581 ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt tccctctttt
     2641 ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga aaaacttgtt
     2701 aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat tttgagtctc
     2761 aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac gaagaaacta
     2821 tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca gaaagacaaa
     2881 gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt aaaagatcct
     2941 atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa cggtgtactg
     3001 attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg gcgtttaatc
     3061 agctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct tgaaaatatt
     3121 gtcgagactt ctaaaagaac ttagaatcat tagaaaaata aggagtagac ttgatctatc
     3181 aacaatttcc ttcggggagt gacggggtag atttttacga ttggtaacgt aaaaaattgg
     3241 tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta aataatatac
     3301 acagagtaaa caagagggaa tttagaggtt agacaagtta gaatagcgcc tagattaatc
     3361 atgagcagct actaagggat ctaggctttg acgcaaactc atggaataag gagtaggtct
     3421 tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact
     3481 tgcgaggacg ttggtcattg gatcaatcga gcatttaccg ctgtctacta gggtttctcg
     3541 tttcggacgc cataaacgag tcgatatttt ttatgtattt ttaagtaaac gaaaaacgat
     3601 aaaatatgaa cgagtcctga ataaagtgaa aaaattgata taaaacatag agaaaatgaa
     3661 atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg attgggcaca
     3721 catactatta aggtttcttt ccaacgttgg gattacaaag gttttgtgac gtttagaaga
     3781 ggcggaaatt gtaaaggatt agatgtttta gcattaaacg aggatgattt atacgatcaa
     3841 acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga aggtaatgaa
     3901 tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga agatacttgg
     3961 tcatatttga gtgattatat tgtcagcttt gaaatcattg aatttgtagc ggataaagaa
     4021 gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc acgacgatta
     4081 ataaaaaggt agtattacct taggcattga gtaaggaat
//
LOCUS       DAPBAB010000136         3958 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPBAB010000136 DAPBAB010000000
VERSION     DAPBAB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.148"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(268..843)
                     /locus_tag="RD444_002877"
     CDS             complement(268..843)
                     /locus_tag="RD444_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550180.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            989..3913
                     /locus_tag="RD444_002878"
     CDS             989..3913
                     /locus_tag="RD444_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDT7550181.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
BASE COUNT         1341 a          697 c          733 g         1187 t
ORIGIN      
        1 aaccaaatcg gcaatttcat gttcatcatc cacaataagt attttatcgc tcatagttat
       61 caccctttca cataagtttg cccttaataa tttatcagat tatagggccg aatttataag
      121 catttacata ataaatgact aagaatttct taagatgatg attctttttt tatcaaaggg
      181 cttttcggca tcgctaggag gctgttaata ttttatagtt tccttttctt ctttaaaaat
      241 attgcccatt agcggaatac agaatggcta attattcact tctgataatt tcctgtataa
      301 tgaagcccta gatacattag taatttcaca aatttgattt acagtcatat ttccttcttt
      361 atatagcttt accgcataat tcattcctgc gtgattttta tgatacttct ttaatcgacc
      421 tttaaacttt ccttctttct tagccaattc aatcccttca cgttgtctca tccgaataag
      481 atctcgctct aattggttaa caccagccat tacagtaatt aagaattggc tgtatggatt
      541 atcttctgat aaatcaagcc atgtatcttt tagtgatttt aaacttgcct ttttatctcg
      601 tatgttatcg attaattcaa atagatcttg tgtactacga gtgattcgag ttaagtctgt
      661 aacataaatg atgtcatctt cctgtaaatc gtctaacact ttttgaagtt gctcgcgatc
      721 ctttgttgct cctgaaactt tctcttcata tataatatcc attccgatct cgttcaactg
      781 ctgaaattgt cttgaaggat tctggttagt cgaactgaca cgaatataac cgattttccg
      841 caaaatatca cctcattttt gagacaagtc ttatgagacg ctcttaacta tgattttatc
      901 agtctactac atttgtatca atagagtaca ctctattgat atataattga actaataaat
      961 tgaaaataca gaaatggaat gatactgaat gaaaattgcg agaggtagag aattgcttac
     1021 accggaacag agacaggctt ttatgcaaat ccctgaagat gaatggatac tggggaccta
     1081 cttcactttt tccaaacggg atttagaaat agttaataag cgaaggaggg aagaaaaccg
     1141 tttaggattt gctgttcaat tagctgttct tcggtatccc ggttggccat acactcatat
     1201 caaaagcatc ccagattcgg tcatacaata tatatcgaaa cagattggtg ttagtccatc
     1261 ctcgcttgat cattatcctc aaagggaaaa tacactttgg gatcatttga aagaaattcg
     1321 aagtgaatac gactttgtaa cttttaccct gagtgaatat cgaatgacat ttaagtacct
     1381 tcatcaatta gctttggaaa atggtgatgc cattcatcta ctgcatgaat gcatagattt
     1441 tctaagaaaa aacaaaatta tactgcctgc tatcactaca cttgaaagaa tggtgtggga
     1501 agcaagggca atggctgaaa agaagctatt taatacggtt agtaaatctc taacaaatga
     1561 gcaaaaagaa aagcttgaag ggattattac ctcgcagcat ccatccgaat ccaataaaac
     1621 gatattgggt tggttaaaag agccaccggg tcatccttca cccgaaactt ttctaaaaat
     1681 aatagaacga ctcgaataca tacgaggaat ggatttagaa acagtgcaaa ttagtcattt
     1741 gcaccgtaac cgcctgttgc agctgtctcg cttaggctca agatacgagc cgtatgcatt
     1801 ccgtgacttt caagaaaata aacgttattc gatattaacc atctatttat tacaacttac
     1861 tcaggagcta acggataaag cgtttgaaat tcatgatagg caaatactta gtttgttatc
     1921 aaaaggtcgt aaggctcaag aggaaatcca gaaacaaaac ggtaaaaagc taaatgagaa
     1981 agttatacac tttacgaaca tcggacaagc attaattaaa gcaagagagg aaaaattaga
     2041 cgtttttaag gttttagaat cggttattga atggaatacc tttgtctctt cagtagaaga
     2101 ggctcaggaa cttgcacgtc ctgccgacta tgattattta gacttactgc aaaaacggtt
     2161 ttattcacta agaaaatata cgccaacgct attaagagta ttggaatttc attctacaaa
     2221 ggcaaatgag ccacttttac aagctgttga gattatccga ggaatgaacg aatctggaaa
     2281 gcgaaaagtg cctgatgact cacctgtgga ttttatttca aaacgatgga aaagacattt
     2341 atacgaggat gatggtacaa caattaatcg tcattactat gaaatggctg ttttaacaga
     2401 acttcgggag catgttcggg caggagatgt ttccattgtt ggcagcagac aatataggga
     2461 ttttgaggaa tatttgtttt cggaagatac atggaatcaa tcgaagggga atacgagatt
     2521 atcagttagt ttatcattcg aagattatat aacggagaga accagcagct ttaatgaaag
     2581 gttaaagtgg ttagctgcca attccaataa gttagatggg gtttctcttg aaaaaggaaa
     2641 gctatcactt gcacgcttag aaaaagatgt tccagaagaa gcaaaaaaat ttagtgcaag
     2701 cctttatcag atgctaccaa gaataaaatt aactgattta ctcatggatg tggcccatat
     2761 aacaggattt catgagcaat tcactcatgc ttccaataat cgaaaaccag ataaggaaga
     2821 aacaatcatt atcatggctg cccttttagg aatgggaatg aatattggct tgagcaagat
     2881 ggccgaagcc acacccggac ttacatataa gcaactagcc aatgtatctc aatggcgcat
     2941 gtatgaagat gccatgaata aagcccaagc catattagta aactttcatc ataaattaca
     3001 attgcctttc tattggggcg acggtacaac atcttcgtca gatggtatga gaatgcagct
     3061 aggtgtttca tcactacatg cagatgcaaa tccacattat ggaactggaa aaggagccac
     3121 catctaccga tttacaagtg atcaattctc ttcttactac acaaagatta ttcatactaa
     3181 ttcaagagat gcgattcatg ttttggatgg tttgttacat catgagacgg atctaaacat
     3241 agaggaacat tatacagaca ctgccggtta cactgaccaa atattcggac tgactcattt
     3301 attaggattt aaatttgccc caagaataag ggatttatcg gactcaaaat tatttacgat
     3361 agataaagca agtgagtatc caaaactaga agccatttta cgtggacaaa taaatacaaa
     3421 ggtcattaaa gaaaattatg aggatgtttt gcgattagct cattctataa gggagggaac
     3481 agtttcagca tcccttatta tggggaagct aggttcctat tcaagacaaa acagcttagc
     3541 tacagcctta cgtgagatgg gccgaataga aaaaacgatc tttattttga attatatatc
     3601 ggatgaatca ttaagaagaa aaatacaaag aggattgaat aaaggagaag ccatgaatgg
     3661 attggcaaga gctattttct tcggaaaaca aggtgagctt agagaacgca ccatacagca
     3721 tcaattgcaa agagccagtg ctttaaacat aattatcaat gctataagta tttggaatac
     3781 tctccaccta acaacagcag ttgaatataa aaaacggaca ggtagcttta atgaagattt
     3841 gttacaccat atgtcgccct taggttggga acatattaat ttactaggag aataccattt
     3901 taactcagag tgaactgccc cctgtcaagt agacaggtaa aaaaacaaag tattttag
//
LOCUS       DAPBAB010000137         3956 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPBAB010000137 DAPBAB010000000
VERSION     DAPBAB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.149"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            1203..2126
                     /locus_tag="RD444_002879"
     CDS             1203..2126
                     /locus_tag="RD444_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7550182.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2142..2681
                     /locus_tag="RD444_002880"
     CDS             2142..2681
                     /locus_tag="RD444_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDT7550183.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3366..3539
                     /locus_tag="RD444_002881"
     CDS             3366..3539
                     /locus_tag="RD444_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550184.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYN
                     SPNEELFVKETDK"
BASE COUNT         1489 a          602 c          674 g         1191 t
ORIGIN      
        1 ggctaatttt caatataagc ttacaaaaaa gtattcttta tctaattcct atagcattta
       61 tcttagcaat ttttagaagt atttacggaa ataaaaactc taaatagcta aaaataaatc
      121 atactaaatt ttacaaaaag tggctagcat tttctagcca ctttttgtat tgtggagcgt
      181 gggttttgag ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc
      241 aaatacggag gtatttggct agcaaatata gagcttgcca aaccctttta gtgagttttg
      301 tagaaagtac ctttaaaggt actttcacat tacgaacgat acgtccgtag gacgtactga
      361 aagggtggga cttatgagca aattaatgca aaatttggct agcaaaaaag ctaaaaaata
      421 tatgctaata atcgagcgaa ataaacactt tttcaagtga gcgaatccgc catgacaaag
      481 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccga cttttttaaa agtctaggca
      541 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgcctaag
      601 tatataaata tataagaata ggtttaagag tgcttaaatc gaattttaga gggcatttag
      661 acaattttag ggttgttagg gcttgccctg accgcctgta aggcgtttta agaacaagat
      721 aagggattta tactaggact gtcttaaaat gtcttaaacg aacgctcaga gcttctcaaa
      781 gggttcctta tgaacggctt ttagcaaagg agccggaggc gactgcgcag tataatgtga
      841 gagcagagcg atcacacctt tttagcgaac cgtagggagc aaggtttgga attggcagtt
      901 ttgataaatt cgcgaacctg tttagagaaa aaaggagtga ttttttaggc gggaaaatta
      961 gcctcccctc tttttatatc tctttttata tctcttttta tatctctttt atgtcggctg
     1021 tgtccttact ctcccaagca attcagagtt ttttcaagta ggttttctac gcttttcaag
     1081 taggttttct acgcttttca agtaggtttt ctacgctttt caagtaggtt ttccgacatt
     1141 attgagtatt tttataaagt atgttacttt tatttcatac tttataaaag taggagaagc
     1201 ttatggcaaa tgaaattgta aaatatgaaa atagattaaa tcatattcct ctgagaaaat
     1261 ttaatagtcg tgaaatgaac ttattttttt caattgcttc acgtgtacgt gaaaaaggtg
     1321 tagatgagat tattttttca tttcacgatt tgaaaaaact aagcggttat acagaacgtg
     1381 gggaacaatt tataaaagat cttagtagca cgtatgacaa attattaagt attaatgctt
     1441 ggaatgatga tggaaatact ttaactaaat ttgttgcttt cacaaaatat agcattatac
     1501 gagataaaca acttgttata atttcagtta atccagaatt taaaggactt tttaatcaat
     1561 taaagaactg gacaagattt aatctagagc aatttactaa tttggatagc acatattcaa
     1621 aaactatgtt tcgattatta aaacaatatc gaactcaagg ctggactgaa ttttccaaag
     1681 aaaaattttt tgatttacta gatattccta aaagttattg gaatagtccg tcaaatattg
     1741 acaaatttat cttaaggcca attaaagaag aactcactcc cttatttaaa ggattaacta
     1801 ttcgtaaaaa atatggtaaa ggtcgaggta aacctgttat cggttatcgt tttacttgga
     1861 aagctgaaat taaccatgct gacgattttt ccaaagggaa acaagaagat ttacgtataa
     1921 aattatttaa tattgatcac aatggagaat taacacgaga agaaaaatgg cgcgctaaag
     1981 atcgaatact aaatcttccc ttaggcactc atgaagctga ttttaaacaa caacaacaaa
     2041 ctgaagaaaa acaggcagaa aaacgagaac tttcaaacaa actcaaacaa gatttactag
     2101 gaaatttaca aaacttattc gattaagctt aaggagtatt tatggcagat aaaacaatca
     2161 aagaacttgc ggaagaatta ggagttagta aaacagcaat taacaaaaaa gtttctgaca
     2221 aaaatcgaaa actttggttt gcaaaaaacg gaaacaagtt tgtgattaac gaaaccggtc
     2281 aaaaagctat aaaatcaatg tttttagtcg ataacgcaaa cttaaatcga aaactagttg
     2341 gcgaaaaatc aaaaaccaaa aaccataaaa acaccaacca agtttccgaa ttaaaactcc
     2401 tttctttcct agaaaaacag ttaaatcgaa aagatataca gctacaagaa aaagataatc
     2461 aaataaaaca aatgcaaaaa ttactcgacc agcaacaaca actcactcta caagcgaacc
     2521 aacaaattga gaacctacaa gaacagctcc atttaactta tacagaagaa actccaacaa
     2581 ccgaatcaac tgccttttct gaaaaaaaag cagacaatac acaaccactt attgaaaaaa
     2641 agtggtggca aatttggaaa tctaatagaa ccaatctcta aaattttcta gaagttagtt
     2701 cggatagtag aggaaaaagt tgtactacct gatggggaga tcactcaaat ttttatgaga
     2761 aagaaaaaat agtttacaac tgtaaacaat caatgtttat agttgtaaac tatcaaacta
     2821 tatggtgact tattgcttaa aataggttga aatctcactc aatttttggt ggaaaaacaa
     2881 aataaattct aatcgatttt gttgaaaaag tcataaaaaa acaaatgtga ttttaacaaa
     2941 atttcaaaaa aatcgtttga aatttcgttt attttttttg agaattttga tcatcgtagg
     3001 gtcattgtag aatcattatg agatcatcgt gggatcatcg aataaagtat gggaaaggct
     3061 tgcggtgctt ggtattaagg gcattctccg cctcccaggt tcttgaccat tttctctcaa
     3121 cgtggtaaaa atcccactta taagagaaaa tggtcaagaa cctaaacggt cggcgaagaa
     3181 aacataccaa gcaccacaag caaaaaaagt tagagtaact tccaaaaaag aaaaattttt
     3241 tagaacaact tttgaaaatt taattttgat ttatatccgt tcaatttaaa catttgataa
     3301 actgaaggct agtatttaaa atgtgtatac aagtggtagc gttaaaaaaa gtggaggtat
     3361 aacctatgtt aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt
     3421 ttagccccat gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa
     3481 attcaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     3541 aaaaaagact gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     3601 gcacgcgctt atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt
     3661 aaaaaaatga cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag
     3721 ctttagaaag ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca
     3781 ttggtagagg ggctacttgg catagtgtac attatgtcta ctaaccttta aaataacacg
     3841 ttttataaaa taaaccctct tgacaatatt taattttctg attattctaa atatatacag
     3901 aaagcgcttt cctaggattt tatatctaag gaggaagtaa caatgaaaaa taaaat
//
LOCUS       DAPBAB010000138         3925 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPBAB010000138 DAPBAB010000000
VERSION     DAPBAB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.150"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..301
                     /locus_tag="RD444_002882"
     CDS             <1..301
                     /locus_tag="RD444_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDT7550185.1"
                     /translation="ISLKDICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSY
                     KAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            312..2516
                     /locus_tag="RD444_002883"
     CDS             312..2516
                     /locus_tag="RD444_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDT7550186.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            2537..2686
                     /locus_tag="RD444_002884"
     CDS             2537..2686
                     /locus_tag="RD444_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550187.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            2762..3355
                     /locus_tag="RD444_002885"
     CDS             2762..3355
                     /locus_tag="RD444_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDT7550188.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            3371..>3925
                     /locus_tag="RD444_002886"
     CDS             3371..>3925
                     /locus_tag="RD444_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDT7550189.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDN"
BASE COUNT         1473 a          671 c          810 g          971 t
ORIGIN      
        1 cattagtttg aaagatattt gtagagaagt cgcaaaagaa ttaaaagatt gtgaacgctc
       61 gaatggtacg tatgattttg atcgttttta tcaaggaaaa ggggatatta attctcagat
      121 tcttttctct tataaagcag aacaatacta caaaggctat aaaaatcatg aacatgaatt
      181 tgtacctgaa gtgaaaaagg attggaacga tcaattgaag catgagatcc atcaacagga
      241 aattagaaag caaaaacgtg cgatgttgtt tcaacaaggc agacaacaag aaagggagtg
      301 agatcgtcac gatgaaaact gtcattttag cagaaaaacc gtcacaagcg aaggcctatg
      361 ccgatagctt ttctaaagcc acccgaaaag acgggtattt tgaaatccaa gatcgattgt
      421 tttcgggtga aactgtgatc acgtatggtt ttggtcattt agtcgagtta gattcgcccg
      481 atatgtacga tgaaaattgg aaacaatggt cattagagca tttgcctatt ttccctacac
      541 attatcacta tcatgttcct aaagataaga aaaagcaatt caacgtggtg aaacagcaac
      601 ttcaatcggc cgacaccatt attattgcga cagatagcga tcgtgaaggg gaactgatcg
      661 cttggacgat cattcaacaa gcaggcgctg atcaaggaaa aacctttaaa agactttgga
      721 tcaactcctt agaaaaagaa gccatttatc agggattcca gcaattacga gacgcagaag
      781 agacctatcc caaatttgaa gaagcacaag cacgccagat cgctgattgg ttgatcggca
      841 tgaacggcag cccactgtat agtttactct tgcagcaaaa gggcatacca ggaagttttt
      901 ccttaggcag agtccaaacc ccaacgcttt acatgattta tcagctgcag gaaaagatta
      961 gaaacttcca aaaagagcct tattttgaag ggaaagccca agtaaccgct caaaatggca
     1021 tatttgatgc gaagcttgat cccaatgaaa cccaagcgac gcaagaagca tttgaagcct
     1081 atttaaaaga aaaaggtgtt caagtaggaa agcagccagg cacgattctt gaagtcgaaa
     1141 ccgagaaaaa aagcgcggca agtccacgtc ttttttctct atccagcctt cagtctaaaa
     1201 tgaatcagct catgaaagct agtgccaaag atactttgga agccatgcaa gggttgtatg
     1261 aagggaaata tctaagctat ccacgaacag acacccctta tattacagaa ggagagcatg
     1321 cgtatctgtt ggatcattta gatgaataca agcatttttt aaaggctgaa tcattcccta
     1381 ctcctattca tacaccgaat agtcggtatg tgaataataa gaaagtccag gaacattacg
     1441 cgatcattcc tacaaaaaca gttatgacag aagcaacgta tgaaaagctg acacctttac
     1501 aacaagcgat ttatgatcaa gttttaaaga cgacggtggc catgtttgcg gaaaaatata
     1561 cctatgaaga aactacgatt ttgacacaag tccagcagct tcagctaaag gcagtcggaa
     1621 aagtaccagt agatctaggt tggaaaaagc tatttggcaa agagagcgac agaaaagaga
     1681 aggaagaaga accgctactt cctaaagtaa caaaaggaga aacggtgacg gtagatctac
     1741 aaatctttga aaaagaaacc aagccaccac aaccttacac ggaaggtaca ctaatcaccg
     1801 ccatgaagac agctggtaaa accgtggata gtgaagaagc acaatccata ttgaaagaag
     1861 tggagggaat tggtacagaa gcgacacgag caaatattat tgaaaccttg aagcaaaagg
     1921 aatatatcaa ggtagagaaa aataaacttg tggtgacgaa taaaggaatt ttgctttgtc
     1981 aggctgttga aaaagaaccg ctgttaacaa gtgccgaaat gaccgccaaa tgggaaagct
     2041 atcttacaaa gattggtgag caaaaaggga cacaagctac ttttctagca aatatccaaa
     2101 aatttgtttc ccacttgttg gaagtcgtcc caggacaaat ccaagccact gattttggag
     2161 caacgttaca agaggtgaaa gtagccagtg aaaagcaaga agcggcgcgt catttaggat
     2221 cttgtccaaa gtgccaggaa aaagaagtca tactttatca gaaggtggcg acttgcacgt
     2281 ctgaagcctg tgattttaaa ttgtgggtaa cgattgccaa aaagaaactc acagcgacac
     2341 aactgaaaga gatcattcag aacggccgaa caagtcaacc agtcagagga ctaaaaggtc
     2401 agaaaggttc gtttgaagca acgatcgttt taaaagaaga ctttacaaca ggatttgagt
     2461 tttctgaaaa gaagaagacc acctacaaaa gaaaagtgag acgtaaaacg agttagaatt
     2521 gacggaggga aaaacgatga gaaatacaac gcataaagaa caagaaatcg aaaatcaaaa
     2581 agccaaaata cgttcagagc aggagaaaat gcgtgccagc aaacgaagac tacagcaatt
     2641 gaagaatcaa caacggattc aaaaagaact tgaattagat cgataaatcc tcctgttgtt
     2701 aatattaaac taacatgtta tagttatgtt agtttaatat taacattcga gagggaaagc
     2761 gatgaaagat aaagtcaagt gcatttttga gaaatacaat ggtaatctat ccttgaagga
     2821 tgctagaaat gaagggttat cacctatgac gttagatcgt ttagccgaaa aggacaaaat
     2881 agaaaaaata gcatcaggtt tttatacctt aaaagatagt tttgtggatg aattatatct
     2941 cgctcaaagt atctatacta gaggaatctt ctctcacgag acagcgcttg atctttttca
     3001 agtaggaaca tatatcccta aaaaaattaa tatgatgttt cctaaaggat acaatgtttc
     3061 gaaagagaat ttagatcagt atgccattca accgcattac acgaataaag aaaactttga
     3121 actgggaatc acagaggcag aaagctttta cggcaataaa attttcgtct atgatttaga
     3181 gcgaacgtta tgtgatatgt ggaatcccag atataaagca agcgtagaag taaaacaaga
     3241 agcattaaaa gaatatatga caagtaccag cagaaataca aataagctaa gaagatacat
     3301 gaatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     3361 gtaaaggtga atgacaccga cacaatttaa agcgaaaaca agaaatgttg cagctgagaa
     3421 acatttaaac ccacaaattg ttcaaagata ttatatgatg gaaaaattat tagaatgtat
     3481 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga tcggatcaaa
     3541 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     3601 aacaaaagaa aaattagaaa ctgttttttc tgaattaaca gctgctccta caaaagatgg
     3661 cattcggttt gaattaaagg gcttaacaga gacgagggaa gccgattatt acccaggttt
     3721 tcaagcaaag tttattgctt ttcttgaaaa tgcacgtgta ccttttaaat tagatattac
     3781 ttcaggtgat tctattattc cagcagcatt gatatatgaa cataaattga tgtttgaaga
     3841 taaaacaatt catattccag cttatcctac ggaacaaatt ttagctgaaa aattacatgc
     3901 cacgtttagt ttcggaatag ataat
//
LOCUS       DAPBAB010000139         3783 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPBAB010000139 DAPBAB010000000
VERSION     DAPBAB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.151"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..1175)
                     /locus_tag="RD444_002887"
     CDS             complement(<1..1175)
                     /locus_tag="RD444_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7550190.1"
                     /translation="MKKSAKDRVFDELIRLTGNKSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWYSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNE"
     gene            1658..1873
                     /locus_tag="RD444_002888"
     CDS             1658..1873
                     /locus_tag="RD444_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDT7550191.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            2734..3693
                     /locus_tag="RD444_002889"
                     /pseudo
     CDS             2734..3693
                     /locus_tag="RD444_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1196 a          770 c          596 g         1221 t
ORIGIN      
        1 ttttcattga aattaccttc atttatcagc tgagtaattt tttccaaaat cgaatcataa
       61 tcaaacggtt tgattcgatc cgtttttatc ttttgatttt tctttatcaa taaactagca
      121 atcggcaagc tttctacttc ggattccagc atatttaact gaatcaagat ctcatttttc
      181 ccaatcgtta atggctcttc ccgtgttcga ctgtacacat ccgcacaact gattttaata
      241 atattcttca gcttaccaat attcccacta aagggatgag ataacattag agacaaagct
      301 tcttttgaaa caataatatc tttttgtaaa atatccgctt cattttgaaa aaataattga
      361 atcagctcta accgttctcg aatgggacga tcttcaaaag ttggcaacat aacactgatt
      421 tgaatacgtc gatcaaaggt gtctaataaa taatcttcac ttttttccgt cgtggcgaag
      481 ataaaacgaa ccttagcaga ataccagttt acattttcac cgactggacg ataatttcca
      541 gtatcaataa aaacaaagag cttttcttga ttttctttcg acaaacgatg gacctcatca
      601 agaaaaagat agccattgtt ggctgttgcc aataacccct ctgtatcctt ctcagccccc
      661 gtaaaggccc ctttcttgta tccaaacagt gttgccgaaa gcagttcggg attgtccgca
      721 taatcggcac aatttaaaat agcaaatggt gcatcttctt caataatttg ctcgtggata
      781 gcgtattcaa aaatttttgt ggctaaaaaa ctttttccaa cccccgtggc tccatttatt
      841 aatacactga gtccgttagg cggatatttg acagctgcaa tacatttttg aatgacctct
      901 ctcagacttc cgtcatgacc caccacagaa tgaaaggctt cgtttatttg ctccgtatta
      961 tctacaaccg catattcatc taaaggcttc cacaaaacag gctttcccgg taatttctcg
     1021 acctttttat cttccactaa tctcgtcaaa taatggctgg cattctgacg agagatacct
     1081 agattcttcg aaattttctc ggcagtgaca gaagacttgt tccctgttag tcgtatcagc
     1141 tcatcaaaaa ctcgatcttt agcgctcttt ttcattttcc cctccagttc aacgtgtcta
     1201 gaagcataat aaactatcgg ctacaatatg tctattctat atttctgttc caattttact
     1261 ttgaaacaaa aataatcgag cttaaactga tattagttat aaaactatag ttagattacc
     1321 aatcttcctc tcatacaata aacttctctc acctgcatcc aacctactta tgtgaaagaa
     1381 taactgccga ttatttatct tttttacagt tactttcgaa cataaaaaaa tcaccttcaa
     1441 atactagaaa aactactatt tgatggtggt ttagaacaat ttccgttgat tcagtatcgt
     1501 ctggtcccac atcctctgtc tcattaatca caatagcata ctccaaagga taaagctggt
     1561 tgatatctca atgcctttca cacctatcaa ttttgtcaca cttatctttt cctaggttct
     1621 attctatata atctagaaaa acaaagaagg agacaccatg aaaaaattca ttattccttg
     1681 tatatttctt ttcttgagta ttttgttatt ttcaacttcc atcctagtcg gttcttacct
     1741 cgattcaagc ggaatgctga ttgaaccagc gttcttttgt gttccgttgg gctacttaac
     1801 attaggactt gctttcttta ctgccttata cagtttcgtc aaacaaagat tattgaagaa
     1861 atactcggct tgatttgcac gtaaatcctc tgttgaagaa aagttagttt tttcaacaac
     1921 gaaccttcga gtattaagaa tggatgttga tgtagacatc aaacgtctgc ataaacattc
     1981 attttattcc tccattacca acaattattc ccaatcgcca aaagaagctt ttttcggaat
     2041 taccttctcc attttttcac ctgctggtta tattgtcttt tgtaataccg atagtcctct
     2101 tcaatcattt tcaatatgcg aaaagaacca attttaacat ggcactcaaa tcacaattcc
     2161 tagggcggcc aaaaagatac ggctcgttga tttcaagggt ttcaacgagt tcattgacaa
     2221 acccagcggt gctatcttct tttggaataa gaagttgtga tatctaaagt gagttgattc
     2281 atgttataat ttgtatgcat gattttgatc ctttcaaggg ttgaatgatt tagagtgcga
     2341 gtatcctctc gtactctttt attttaccta aaaagctcca gaaatgacga atgactcgtc
     2401 atttctagag cttttttcag agaattatgt cacaatctct ttttgttata ttgatattct
     2461 atatcttttt ttattggttt tattcccttg ttcgttatag actaaaatcc ttctttcagc
     2521 attattcaaa tcaaatggca acataataga gaaatttata gtatgtataa tcatctgaat
     2581 tcaatcaaat caaccggagt catatacgga gtcaaactat cccactgaga gtttattaaa
     2641 cgcagattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca ctcaaaatag
     2701 ttccgaccaa agaattataa ggagtgaaga caaatgacct atacccatct tacaacaaat
     2761 gaactcgtga tgatagaagc ctatttcaat atgcatcaat ctgttaccgt agtcgctaag
     2821 acctcaaatc gttccagaca gaccatctac aatgtgtaca acttcctgaa acaaggccaa
     2881 tctatccatg actattacca acaatatcaa aaaaataaaa cacggtgcgg ccgacgaccc
     2941 atttgtttgc cagcagaaca agatgcgtat atccgaaaca aggtggcgca aggatggaca
     3001 cccgatgtga ttatcggtcg tgctgagttt tcaatttcat gttctgttcg aaccctttat
     3061 cgcaagttta aaacaaacta atttgattgc tcgaaactac ccatgaaagg aaaacggaaa
     3121 cccaacgggc ataaagaacg tcgagggaaa caagccttca aacggaatat ttcagaacgt
     3181 ctcacggact atcctgaatt tcaacaggaa tttggacatt tggaaggcga cactattgtg
     3241 ggtgtccatc ataaaagtgc tgtcatcacg ctggttgagc gcttgtcaaa agtgattatt
     3301 actttaaaac ccgaaggacg gaaagcacta gatattgaaa aagtgacaac tgactggcta
     3361 caaaagcttc cccgaaactt gttcaaatcc attacctttg attgcggaaa agaattttcg
     3421 aattggaaaa acatgagtaa tgctaacgat atttctattt attttgcaga tcctggaacg
     3481 ctgtcacaac ggggattaaa tgaacattct aatggattgt tacgtaaaga cggacttcct
     3541 aatacaatgg atttcaatca ggttgaccaa aaatttgtgg cggcggttgc ctccaaaaga
     3601 aatcatatac caaggaaatc tttaaattat cgaacatcgt tagaagtatt tttgagttac
     3661 ataagtgagg agcaattgtc taacttaatt tgacatttag aaaataataa accatatccc
     3721 atctattcaa ataatatttt accgattgaa agacttgcta atcaaagcag aatagtttat
     3781 ata
//
LOCUS       DAPBAB010000140         3677 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPBAB010000140 DAPBAB010000000
VERSION     DAPBAB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.152"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..2448)
                     /locus_tag="RD444_002890"
     CDS             complement(<1..2448)
                     /locus_tag="RD444_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550192.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSG"
     gene            complement(3120..3388)
                     /locus_tag="RD444_002891"
                     /pseudo
     CDS             complement(3120..3388)
                     /locus_tag="RD444_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1010 a          827 c          587 g         1253 t
ORIGIN      
        1 cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc ggttttcttt
       61 cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgattg gtgtcgcatc
      121 taattcatac cctattggcg cttgagtttc taccagctga tatgctcctg gtgctaaacc
      181 gtcaattgcc agttttccat cttctcctgt agttaaaccg gtttgtagcg tttctccttc
      241 tcggttctgt aactcaaaaa cagctccttg aaggacttca ccactgcctt cagcatgttt
      301 aattagttca acaccacccg tatcaaggat gttttcaatg gttaacgtca cttcttctat
      361 ggagtattca tttataaaaa catcatgtac cgtcgcatct aaaagatacc cttccggtgc
      421 ttctgtttct cggacttgat agctacctac aggaagcggt tcacttaaaa attctccgtt
      481 ttctgccgtt ataccttctt gtatcacttg tgaatcgctt gataaaattt caaattttgc
      541 tcctgccagc ctttggccat tctcatcagt tttaattact ttaatacgcc catcgatttt
      601 ccttgttctt acagtactcg tattcaatcg attgtcacta ttcacaagcg ttgcaaagtt
      661 taaaatttga tcgccgaccg catacgcctc tacattgacc ttaaaagaga gggtaaaact
      721 tgtcggacca ccaatcgtat tattcgcact gaaacgaact attccattat tgacttcggc
      781 atcaatgaaa gagggaatat ctgatagaaa tgacacaaat tccatgcctt cttccaaaac
      841 atcttcaata acaagcgtat ttcttgttcc agaaaacatc gagacatgca gagtatacgt
      901 tagttcattt tcattgagat tgataacatt tttgtccaca ctttttctta aagagattgc
      961 cggaagatta ctttcaggga ttctcaagcg gtagtctcgg ctcattacct gattattgta
     1021 ggtcatactc gctcgattcc gagaaaaccc atcctcttgt cttccttcat tgaacaagtc
     1081 gacatcggtc acttcaagag catattcaat aaaatacgat tgaccctctg ctagattacc
     1141 aaagtccaca acaagtgtat tcgtttcctt attaaaagat actttgtcca caaattcctc
     1201 ggaaacataa tgagttctaa cgtttgcttc acctggattc gttacttctg cgatccgaac
     1261 cccacctgaa ataggttctc tatttccagt ggaactccat gtagtcggat tcggtgccaa
     1321 agttgttcct tgcggtagta catcggtaac cactacaccg tcttttcgtg atccttcata
     1381 atttacctgc aaaataaatt tattactgtt ggtcacgtct tgcccgtcaa tcagcagatt
     1441 cccaagattt ccttggctgt cgatttctct agagccatag tataatttcg aaaaaatggg
     1501 tcttgatgaa ggaataggcg taattgaaaa ttccgtgttt gctgctcgct cagcataagt
     1561 aagactcact tgaaagtcat tatttggctc agtaccgcct gattgaaaga aagcaacttc
     1621 aaacgtgata ctgaatgtat acgactgagc ggcttcattt aatccttcat aatcaaaggt
     1681 aaatggaaaa acaacgtctg tgccttcttg gtaaaatgcc agtggcttta aaggtgaatc
     1741 atcagcgacc tccactctaa tgaactcaat taagccaatc gatgctctag gaactcgtac
     1801 gaccatctcc ccatattctt caagaatatc gccagcgcct ccttgaatgt atccctttaa
     1861 cgtttgtgta tgcctgctcg gcaaagtggg gcgctcagca gagatgccga tgtcataatt
     1921 caagagctcc tcttgtcttt caatctgaga ttcctgcgtt atagatggct cagatgatct
     1981 ggcttcatta aaagattcac ttaagctttc ttctggtata ttaaaagatt cacttgtgct
     2041 ttcttctggt acattaaaag actcacttgt gctttcttct ggtacactga tttctattga
     2101 gaccgtattt gattgaatat caccgcttct tgcggtcaat tcgtatttac ctgaatgctg
     2161 ggaagaataa gtaatcagac gttcgtaatc attgagttgc ttcacttccc cttcaatcaa
     2221 atcctcactc ttatcaatga ccaatgcatt tagcaggtct ggtgttcctc tgactcgcaa
     2281 ctggaactgc tcacccaaag cagcctcctc ttgatcaact tcgatcgtca gcgaacgctc
     2341 tggctgttct gctaaagact cagctagaac cgttatgttc ccaaattgat tgatcacaat
     2401 aagcataact agaaacatac ttaacagttt atgaaatttg ttcctcacta ttactccccc
     2461 ttataatttt gactaaacaa caaggtaccc gataattttc taaaaagatt ccccaactca
     2521 aaatggctgc gttacctccc tcgtattttc ggttcaactc aagcgttgca atgtaagctg
     2581 taaacaaagc taatgattgc cgattgtctt ctgttgattg attcgatagc tttgtatgac
     2641 ttttagttgt ccgtgtggct taaccataat tatgatttca cgattcctcc tgccagtgcc
     2701 ttgcacaagc tggagcgtaa ccctaatagt tattttgtta atgtacttat atacttttat
     2761 acttttccat tagaaacaat cactaaatcg ttgataccaa ggtcagactc tagaatttca
     2821 tcagtgagtc cctgtattgg ttaaccttca tcaatcccaa aaaaatatat caatgcttag
     2881 catcaaaatg aatatatagt ttagaatact tgggattcat cgataactga tcgcttattt
     2941 ggccatatta tttggcactt ggttcaacta aacgcattta ggatgttcct tcattctcgt
     3001 catggatgtt ctcaaaccgt cattactaat aaactgtcca ccgaatcgat ggttcatttc
     3061 ttgcattttc cacgtccaat tactcttttt gctctttatt tgggcgtagt ttcactttct
     3121 taacttgtat cgtcttccat caactcctta atcatcttct tagctttatt ggctcggttt
     3181 ccttgtaaaa ggtatctaca aatagtgaca atctgtatta aatcttcatc attttctgta
     3241 atgtcaataa tttctatatt ctgtaccata tattgcaaag ggtttcacta agttcataac
     3301 cgaagcgaat taaacaactt ttgtatagaa taactacctt ttctattttt ccattggtaa
     3361 ttctatctat taattggttt aatccttttg ttgtagttga ttccactacc aagatgattc
     3421 tctcgaattt taaagcttga agatgtattg ctatttttgc taactggcgt atttgcaact
     3481 atttttccct tctattcttc cttcattgat tcccctctaa aattactatc cataaaaaat
     3541 tgcataaatg acaatagtat ttatattagt catactgctt tttaacacag caaattccag
     3601 aaaaacattc gttacatatt tgtgaatttt tccaaaaaat aacattttta taaaacgacg
     3661 aaaaataaat cactacc
//
LOCUS       DAPBAB010000141         3530 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPBAB010000141 DAPBAB010000000
VERSION     DAPBAB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.153"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            6..245
                     /locus_tag="RD444_002892"
     CDS             6..245
                     /locus_tag="RD444_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550193.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="RD444_002893"
     CDS             312..521
                     /locus_tag="RD444_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550194.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="RD444_002894"
     CDS             581..1252
                     /locus_tag="RD444_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDT7550195.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="RD444_002895"
     CDS             1305..3281
                     /locus_tag="RD444_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7550196.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEIVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGVLSAVVYFKKKA"
     gene            3296..>3530
                     /locus_tag="RD444_002896"
     CDS             3296..>3530
                     /locus_tag="RD444_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7550197.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTI"
BASE COUNT         1296 a          536 c          780 g          918 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagat tttttgtttt gtctgaaagg gggtagctgg atgagaaaat ggttatttgg
      601 ttttttagga gtcgctttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga aaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgaacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggt gtcaataaac aagcctttgc
      841 acaaattcag atacctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgagtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttaccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaagc tgctttcagg
     1381 ggtatcaagt acacgcagaa gatgtcacgc aaaaaacgcc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagggaag
     1621 attctgatct aactaacgcg aaagtggacg aaatcgtgaa agacgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttgctgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagacaat acaggatcag gatttttaaa agatattcat ttgtatccaa
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcacagctc
     1981 aaagcaaaca aacaccttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attaggagat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcaga aattgtagtg ggatcagata
     2161 aagaaccaac agccgatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgacggtg tgacaagcga tttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tatcccaaca gacattgcag
     2401 gcggagcaga cggtatcaca gtaaacggcg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaagtgggg ggatacagca ggacaaggac tttcttatgt cgctgctaaa gcaaatatca
     2521 aagtaacaaa taaagatgga tctgtggtat taaaagaaaa tacagattat aaaatccaaa
     2581 attcagaaaa tggctttgtg atcgatttta tcgtgaataa tggccaagta agtgatactg
     2641 tagcaagttt acacggacaa gatcttcaaa tgacttataa catgtatgta aatgatagcg
     2701 cagctgttgc caatccatta acaaatagcg tggatttcgt ttataacaac aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggt gcaaaattct
     2821 taaaagtcga tagcggacta tttggaatag gaatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgctgc taaaaacgca gaaggaaaat attatggtgg attagttgat acggataaag
     2941 acggtgtcaa agaagcagta tgggtagacg atgtagcaaa tgcagcgatc ctaaaatcag
     3001 ataaagaagg tcactttgaa atcactggtt taactgaagg tgaatattca ctggaagaaa
     3061 caaaagcacc aactggttac caaaaattaa cagaagatat tgcatttaaa gtaaacaaaa
     3121 actcatttaa agaggaaaat cgaatcactg ttaaaaataa tcaaaaagca gctattccat
     3181 taacaggtag taatggtttc caaacgtatg tattaatctc ttgcttgttg ctaggagcag
     3241 gggtactttc tgcagttgtt tactttaaga agaaagccta gaaggatcaa aaccaatgat
     3301 tatcaggaag cctaagaaaa agcgaatcat gggtaaatgg atcattgctt tctggttact
     3361 ttcagctgta ggtgtcttac ttttgatgcc tgctgaagcg agcgtggcga aataccaaca
     3421 aaatcagcag attgccgcaa tcgatcgaac aggaacggca gcagaaacgg attcttcttt
     3481 ggatgttgct aagattgagt taggcgatcc tgttggtatt cttacaattc
//
LOCUS       DAPBAB010000142         3458 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPBAB010000142 DAPBAB010000000
VERSION     DAPBAB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.154"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="RD444_002897"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="RD444_002897"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDT7550198.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="RD444_002898"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="RD444_002898"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDT7550199.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="RD444_002899"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="RD444_002899"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDT7550200.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="RD444_002900"
     CDS             complement(2590..3312)
                     /gene="tsaB"
                     /locus_tag="RD444_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDT7550201.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          987 a          757 c          594 g         1120 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaa
//
LOCUS       DAPBAB010000143         3416 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPBAB010000143 DAPBAB010000000
VERSION     DAPBAB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.155"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..171
                     /locus_tag="RD444_002901"
     CDS             <1..171
                     /locus_tag="RD444_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7550202.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="RD444_002902"
     CDS             406..642
                     /locus_tag="RD444_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550203.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="RD444_002903"
     CDS             649..2601
                     /locus_tag="RD444_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDT7550204.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="RD444_002904"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="RD444_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDT7550205.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1208 a          559 c          589 g         1060 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtc
//
LOCUS       DAPBAB010000144         3396 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPBAB010000144 DAPBAB010000000
VERSION     DAPBAB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.156"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            647..964
                     /locus_tag="RD444_002905"
     CDS             647..964
                     /locus_tag="RD444_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDT7550206.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="RD444_002906"
     CDS             complement(1055..1420)
                     /locus_tag="RD444_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550207.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="RD444_002907"
     CDS             1628..2110
                     /locus_tag="RD444_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550208.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /locus_tag="RD444_002908"
     CDS             2307..3197
                     /locus_tag="RD444_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDT7550209.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1172 a          519 c          619 g         1086 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttt
//
LOCUS       DAPBAB010000145         3362 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPBAB010000145 DAPBAB010000000
VERSION     DAPBAB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.157"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            132..1337
                     /locus_tag="RD444_002909"
     CDS             132..1337
                     /locus_tag="RD444_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDT7550210.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMNF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAMKHWGEGGNDNGTITGEWTNPFPGSSLDKSTFSGGQLFGTNPGS
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            1353..1997
                     /locus_tag="RD444_002910"
     CDS             1353..1997
                     /locus_tag="RD444_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550211.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            2008..2814
                     /locus_tag="RD444_002911"
     CDS             2008..2814
                     /locus_tag="RD444_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550212.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            2836..3066
                     /locus_tag="RD444_002912"
     CDS             2836..3066
                     /locus_tag="RD444_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550213.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         1238 a          530 c          703 g          891 t
ORIGIN      
        1 aactcagaac tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc
       61 tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa attgtttgat
      121 ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt aagcgtcgtg
      181 caaagcgacg cttatttttg tggttgtttg gtacttctgc aggtttgatt actattttga
      241 tcactgtgtt cgtgaccctt tttctaatac tggcggcggg ttcgattgat aattctgatt
      301 cagattcaag cagtggtggc gaagctttta caggagaata ttcggaagga ttacccattt
      361 ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat gctgtaggtg
      421 cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt tacgaatcag
      481 cgtttggtac gtcggcgtcc gcaagaaatg atctcaacta ttttgggatt acgtggttcg
      541 atggctgttt gtttcctaaa ggaactgcac gaggaattgg tggtattgaa ggtggctggt
      601 acatgaattt tcccaactca aaagctgcat tctcctatta cggctttatg gtagccacac
      661 aatcaaattt taatgcatgt gtaggaaata aaagccctgg cgcaagctta ttgatcttag
      721 gacgaggtgg ttatgccgct gcaggaatta ctgaagatag tccatattat acaggttgta
      781 tgtcaattat cacttctaat aaattaaccg aatatgatga attcgcgatg aaacattggg
      841 gtgaaggtgg taatgataat ggcacaatta ctggtgaatg gacgaatcca ttccctggca
      901 gcagtttaga taaaagtacc tttagcggtg gtcaattatt tggaacaaat ccaggcagtg
      961 aattccgtcc gaatggcttc catgatggat tagattttgg ttcagttgat catccaggaa
     1021 gtgagattca tgcggttcat ggcggaaaag tcgtgtacgt tggcaatcct ggtatttcag
     1081 gtttgggcgc gtgtgtgatc gtgattaatt acgatggctt gaatatggtg tatcaagaat
     1141 ttgcaaatag tacaggaaat tctcgagtga aagtaggcga tcaagtgaaa gttggtcaag
     1201 taattgggat acgagataca gcacacttgc atttaggatt tactcgaatg gattggcgtc
     1261 aagcacaagg acatgcattc attgatgatg gtacatggat cgatccgtta ccatttttga
     1321 atagcagtaa aaaatagatc aggagaaaaa atatggaaaa taaaacgtta aaaatcgtct
     1381 taacagctgc aattagcgtt agtttaatcg gcaatattgc gttgttttgg cagctgaaag
     1441 aggaacaagc agcaaatacc tataaaattg aagaagtggc taaaaacgcg tctgagaagc
     1501 tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa accatcaatg
     1561 agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca aaagaggaag
     1621 taactgaagc acaaagaaag acggcagaag agttcggtca attggccatt gataaatcat
     1681 taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca
     1741 aattaagtcc tacagacgat gatcaccaac atgatgatta cggaagttac tcctttacat
     1801 taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa aattttgtcg
     1861 tatttgttga ttatacaatc ggcaatccgc aattcaaaga tgtcaaacca caaaaaataa
     1921 aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc atagacttta
     1981 gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagaaag acaacgtttc
     2041 aaaaaacgaa aaagttatct tcaatggctg ggaatcttta tgattctcgg ctttttgatt
     2101 cttgctagtc cgatttatac gaatcagaag gtagatatac cagatgaggt aagcacgaac
     2161 gcctttttaa acacgaaaga gctgacaatg gtttcaaaag aaaaaggagc gaaccagcaa
     2221 ttagtttttg aatttgcgat cgatgatgaa gatcaaagct tgttaaaaga actggctaat
     2281 cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat acgagccaaa
     2341 gtgatcaagg tgtctgatga ttattttgca gtgaagctaa cgcatgtacc agaagaatat
     2401 atcgccatgc gtttaaccat tattcctgaa aaaattgatc caaaagtgga tatgcaagaa
     2461 ccacaagatc tgatttatta cgtgcacgaa gataaggtta aagatcaagt gaaagatgaa
     2521 gattatgaac aacacgcgat caattacaaa gtgaaaggat acaagaaaga acaaaaagca
     2581 gctcaaaaac aaatggaatc tttccaagca accattgatt tgaatgaaga attagtgaag
     2641 aagctaaaag atcaattgcc ttatcaagtc gcagatgatc aggaaaatac tgaaaacaag
     2701 attaatagct accagcaaga aattgaaaca tcgaaaacgc aactgaaaga tcaagaagag
     2761 atcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaagat ttagaaggac
     2821 aggtggagtg aaaaaatgaa gcgaacaaac ttggctgaca aagatacttt cgctgagcag
     2881 aaagtattga ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg tcgagaagtg
     2941 aatttatcta acacaacaat atctgagttg aagaatggtc gaaagaattt agatacttta
     3001 aaattcagat catttgaagc tctatatgta gctgctaaaa aatattttac agctgataac
     3061 aaataaaaaa caggagtatt ttatgtgaaa aaagtatata aaaaatgctt atgaatcagc
     3121 tatatcacct taaccatttg ttaatgagat gggagaaaac attaaccacg attctaacgc
     3181 aaacatcaag cacataaaac atttaacact cctcctttct ggctgaagat ttcaaccaaa
     3241 caggaagaca gcgtatcaat tatagcatac actttttctt aaattcattt ttcatatgac
     3301 aaaatatttc cttctaatcg acaaagttag aaggattttt tattgtctaa atacacaaat
     3361 aa
//
LOCUS       DAPBAB010000146         3332 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPBAB010000146 DAPBAB010000000
VERSION     DAPBAB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.158"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(393..710)
                     /locus_tag="RD444_002913"
     CDS             complement(393..710)
                     /locus_tag="RD444_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7550214.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTIHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(711..965)
                     /locus_tag="RD444_002914"
     CDS             complement(711..965)
                     /locus_tag="RD444_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7550215.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTVQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1225..1443)
                     /locus_tag="RD444_002915"
                     /pseudo
     CDS             complement(1225..1443)
                     /locus_tag="RD444_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1709..1903)
                     /locus_tag="RD444_002916"
     CDS             complement(1709..1903)
                     /locus_tag="RD444_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550216.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(2003..2089)
                     /locus_tag="RD444_002917"
                     /pseudo
     CDS             complement(2003..2089)
                     /locus_tag="RD444_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(2473..>3332)
                     /locus_tag="RD444_002918"
     CDS             complement(2473..>3332)
                     /locus_tag="RD444_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7550217.1"
                     /translation="ITMSEIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHD
                     SYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLD
                     LQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVNNRKEVCD
                     DFKMVYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYS
                     TNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELE
                     QMLSQLIEN"
BASE COUNT         1030 a          646 c          537 g         1119 t
ORIGIN      
        1 atattggact tcctctttta aaacacaaaa ataaatacta ccttatttag gtagtattta
       61 ttaaaagtta cgtcaatttt gattaaaaat atactacttg aacctctcat gagtaaagtc
      121 acgaaatttt tgggaataga gcgtacttga tatcttattt tttcactaat agcagtgtct
      181 cttattcacc aagcgatccc cgtagttcct acggttctta atttttctct agaccaatga
      241 ttgtatcttt agaccttcgg ccataacatg gagcaaaata aaaaggtact aagtcttata
      301 tatcaatctt cgagtaaccg acggttaaca accatatcga gcggcattaa tcacccacct
      361 gtagaggatg agcgactttt gcctaattat tgttaaaagc ctagtttcat ttgtttagat
      421 tggaaaatac gtcctacaag tatctctttt ttttcatggt cgatccgata aaataaggca
      481 tagcttttgc cgatcacgat ccgatatgtt gggatatcca ttccatagat ttcggaaact
      541 tcctcatgta tagttggaaa atttttggtt agttctaaag ccttctggat cgcacctaca
      601 aatcttttta tgctggctgg agaaagagca agctcatttt cccattcttc tattatcgtt
      661 tcaaggctgg tctggaaact tttagaataa actagcgtgt actctttcat ctattcactc
      721 cagccgtatt ttccgaaagc ttttaccatt tcaatttctg aaactgttaa acctttctca
      781 aagtcttgga aactttcttc aatcaatttg cgatcgtgtt cttttgctat ttgagtagta
      841 tctaatatga ataagttgcc ttcttgtttg gcttggacag tgtctccgct ttttaatcca
      901 agagagcttg gaaggatcac tcccacacta ttgccgatct ttctaacctt tagttctttt
      961 cccatgttat cacactcctt ttagtttttg atgaataaat tataacatgt tataacaaca
     1021 attgccaacg gtgtttctta agggattcca ctccatatta ttattggaca aaataagcca
     1081 cttagaacgc tgaaaacata tacaaacgat atcaaaatac gagtgaacta ctcaccatac
     1141 acttccgtta acatccattt aagtaggtat ttttattgta tatagattta acatatgtcg
     1201 gtcggttgat tgatatagct aaagttataa ctcaaccgaa gccgattcat ctacacgaat
     1261 aaagttgcga gtgttctcgg ctaattcata ataattgtta tgtgttccat atagcaatac
     1321 tggaataact ttattttctt gaatctcata agcaactcta tacacggtga ccgcatactt
     1381 aaatccatta gaccaattac ctgataaacc tccagttttt tgctcttact taaatggatc
     1441 aatagcaatt tcatcacaaa cctatagagg acgagtgact tctgcctaat tacgttaaaa
     1501 aatattgaaa gtaactctat gtagcaaaaa atatctagaa tttcatttcc tcaaaactga
     1561 tcgatattgt atctaaaaat atatactacc taaaataggt agtatatatt aaaatatgtg
     1621 cttttgttag aaaaatcaat acataaatta tcatatctgt atatgtcatt taggtatttc
     1681 agttatgtta taatagtttt gctgtctgct accccggcct aatctggcaa ggggaggagg
     1741 agatataatg aaataccttt ttgattttgt ttttgcaatc aagccatttg cagatgtagt
     1801 gtgcagttac ctctgcaaat ggcttgattg caaaattgcc aaaaaagaca gtaagcctac
     1861 cagttaattc tataatatag aaacgtaaaa aaaaccccat cagcctgcac gctgatgggg
     1921 tttggtgata taatgaaata cctctttgtt actcatagtt ataccataat tagtcacgaa
     1981 tgcaaccaat agaaaaaact acttagaacg gtagatcatc gctaataaca atgcattgtc
     2041 caccaaatgg attttgtgaa gcattaaata cattgttatt aattgtcgct gcttttaact
     2101 aggcctctcc ccttctatta taaaagaaga tatatatcat ttcttaatta cctactacct
     2161 taaaccaaaa tttaagtact acctaattta agtagtactt aaacaatcta aaaaatagtt
     2221 atcccaattg ttatgctttt ataatacgat tatttgtaac aaaatgaata ttatataatt
     2281 aaacactacc atatttaggt agtgtttaat tattagcaaa acaaaaaata tttattctat
     2341 tctcttaact tcaactaagt tatgctgcac acctttatat aaatcatatg caactcatta
     2401 gctagacaaa ctacaagcat ggggagcgtc aataattttg tgtaaatggt tcctccctac
     2461 tgtagattgt ttctaattct caatcagttg gcttagcatt tgttcaagtt cgccttcggc
     2521 ctgttgaaag cctttatgac tgcgacctag gaatttttgg ttgtaagtat caaaagacga
     2581 gactaagaaa cgttccatcg actcttcgtt ttggaactgt tccttgcggt ggctgtattt
     2641 cttgatttgc ttattaaagg attcaatcaa gttcgtagaa taaatactct tgcgtatggc
     2701 caaggggaaa tcatagaaag tgagcaagtg atcgttcgaa agaatcgatt caaccacttt
     2761 tggatagctg gttttccatt ttttcgcaaa agcaccacgt gcttccaacg ccacctcttt
     2821 agacgaggct tgatacacca ttttaaaatc atcacagact tccttacgat tattgacacg
     2881 cactttgtga ctgatattac gggaaacgtg tacacaacaa tgttgaaaac gagctttggg
     2941 atagaaccga ctaatcgctc ctaccatccc ctttaagcca tccgtgatga acaggaggac
     3001 atttttcaaa ccgcgctctt gaaggtccaa taaaatttcc tcccaaatcg tgatggattc
     3061 agtcggtgca atcgcatagc tcaatacttc ctttgatccg tccgggcgaa tgccaactgc
     3121 gatatgaata gcttccttgg cgacggtttt ccgctttaac ggaatatacg ttgcgtccat
     3181 ataaatagca gcataactgt catgaagctc ccgcccttta aacgccgtta cctcttctgt
     3241 aaatgatttt gttatattgg acatggtttg gggcgtgtag tgatgcccat acattttctc
     3301 aatcaagtct gcgatttccg acatggtaat ac
//
LOCUS       DAPBAB010000147         3258 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPBAB010000147 DAPBAB010000000
VERSION     DAPBAB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.160"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(58..633)
                     /locus_tag="RD444_002919"
     CDS             complement(58..633)
                     /locus_tag="RD444_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7550218.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(638..1612)
                     /locus_tag="RD444_002920"
     CDS             complement(638..1612)
                     /locus_tag="RD444_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDT7550219.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1636..1680)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1895..2863
                     /locus_tag="RD444_002921"
     CDS             1895..2863
                     /locus_tag="RD444_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7550220.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2960..3253
                     /locus_tag="RD444_002922"
     CDS             2960..3253
                     /locus_tag="RD444_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDT7550221.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
BASE COUNT         1072 a          612 c          645 g          929 t
ORIGIN      
        1 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
       61 gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga ctaatgaaac
      121 aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca agatcacact
      181 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
      241 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
      301 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
      361 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
      421 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
      481 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
      541 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
      601 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
      661 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
      721 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
      781 aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca gtgaagcttt
      841 tgaaaccttc acagatggcc tcctcgataa aataagcaac agctaacgga tcattgatga
      901 cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga gtgatcgctt
      961 ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata gtcggtgtca
     1021 agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg agattctcga
     1081 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggcgaacagt
     1141 taccatggct tttgtaagtt ccacccatcg agacaaaacg ttccatatgt ttcccaatat
     1201 ttgggttgac tttcaaagca gaagcgatat tggtcaaagg acccaaagca atgactgatg
     1261 tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga ggttctgcct
     1321 gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca tgagtgtctt
     1381 gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca ggaatgtcta
     1441 aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct gcagggacat
     1501 tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca gctaatgata
     1561 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc attatttcca
     1621 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     1681 acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc gaatgtaaat
     1741 gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa tgaaaaagaa
     1801 atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg gataatagat
     1861 agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt tcattcttat
     1921 tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     1981 aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc gtttgatgga
     2041 ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag agtgtgaaag
     2101 cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga aactagctgg
     2161 tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac atgattaccg
     2221 aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     2281 cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca tcggaatggg
     2341 atggctagat cggctagatt taattgcctg gatccaactc atcttagatg aacagccgaa
     2401 tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga tggcaagtgg
     2461 tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg tttctgttta
     2521 tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa tggtcatgag
     2581 atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag catcagctac
     2641 gagacagctt ggcagcaatc atctgccttt attagtcatc catggtgaaa gagatcattt
     2701 tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata aagcactgct
     2761 gcttgttccg gaagcagaac acttggaagc atcgatgaaa gatccgaaaa cctattggac
     2821 agtgattttt tcatttattg aacaaagaat aacgatgaat taattttctc ttcttttttg
     2881 taagtaattc gaggataaat aattgtttat agatcaggag actgctaaaa taaggtagga
     2941 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa gaaaaaatcg
     3001 tcttgcttta ttcaatgatg aacaacaaga gatcggagaa atgacatggt cagatgcggg
     3061 cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg gtcaaaagtt
     3121 agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga aaaagatcat
     3181 tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc aagacgtctt
     3241 acgtcagtca taagaata
//
LOCUS       DAPBAB010000148         3196 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPBAB010000148 DAPBAB010000000
VERSION     DAPBAB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.161"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..1225
                     /locus_tag="RD444_002923"
     CDS             <1..1225
                     /locus_tag="RD444_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550222.1"
                     /translation="NILCVLITSFLIKFVSLEAMIKNKTLNSGTNLIVQTIKIVFSVI
                     ITITTISLLNQGNEFKQSQQDILDYKYLDGYFTANGFNSLEYEYALENPEQFEEYSNS
                     MLELYNNHHALLCDYAGSSEAQGEFSKSRPYYIQQTIIANRNYISEFSNIQVNGKSLN
                     EDIYDTPTILVSRKYKSDETFIKEHITQEYFRLMNYNQFYGIPEKERHINEFNIVYVD
                     DNSTIKVNTENGFSDMSEPIIIIDTGNFAGLYYLDSLNTRNLFFQMDSREEFSSLLAE
                     YGLKQLVTAGTLLTPYLMQLENVTFVLKTLTMFAIVFIISLLFILYISNYVDIFVNRK
                     KYATKEIMGFSHFKTLKNRYIFWGIELIASCILTAINYNFACLFAIILIDYIFCELLY
                     RIYILNALYEIEKGA"
     gene            1228..1878
                     /locus_tag="RD444_002924"
     CDS             1228..1878
                     /locus_tag="RD444_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7550223.1"
                     /translation="MSLITLKNIKKVYGKNQILFENLSLEIEKGEFVGLVGKSGAGKT
                     TLLNIIGLITDISEGIITIDGQQKLSVNSRNAMLLRRYTIGYLFQNYGLIEDESVLWN
                     LKLALEYKKMKKEDKLKVINEYLKQFNLSDMLNKKIYQLSGGEQQRIAIIKLILQGSQ
                     IILADEPTSGLDKENEQVVMKLLKELNEKGITIIMVTHNMDLRNYFSRVINVETFR"
     gene            complement(2338..2952)
                     /locus_tag="RD444_002925"
                     /pseudo
     CDS             complement(2338..2952)
                     /locus_tag="RD444_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1132 a          454 c          545 g         1065 t
ORIGIN      
        1 aaacatactt tgcgttttaa ttaccagctt tttaattaag tttgtttctt tggaggcaat
       61 gattaaaaac aagactttaa atagcggtac aaatcttata gttcagacta taaaaatagt
      121 tttttcagta ataatcacaa ttaccactat ttcattattg aatcaaggaa atgaatttaa
      181 acaaagccaa caggatattt tagattataa atatctggac ggatatttta cggctaatgg
      241 ttttaattct ttagaatatg aatatgcatt agaaaatcca gaacaatttg aagaatattc
      301 taattcgatg ttagaactgt ataataatca tcatgccttg ttatgtgatt atgctggtag
      361 cagtgaagca caaggggaat ttagtaaatc tcgtccatat tatatacagc aaacaataat
      421 tgcaaataga aactatatta gtgagttttc aaatatccaa gttaatggaa aatcattaaa
      481 tgaggatata tatgatacgc caacaatatt agtttcccgt aaatataaaa gtgatgaaac
      541 ttttattaaa gagcatataa ctcaagagta ttttcgactg atgaactaca atcaatttta
      601 tgggatacct gagaaggaaa ggcatatcaa cgaatttaat attgtgtatg tcgatgataa
      661 ttctactata aaagttaata cagaaaatgg cttttcagat atgtcagaac ctattatcat
      721 tattgataca ggcaattttg caggtcttta ctatttggat tctttaaata caagaaattt
      781 atttttccaa atggattcaa gagaagaatt ttcttcttta ttagcagagt atggtttaaa
      841 acaattagtt acggcaggaa cattacttac accatactta atgcaactgg aaaatgtgac
      901 ctttgtcctt aaaacactca caatgtttgc gattgtcttt ataatatcat tactgtttat
      961 cttgtatatt tcaaattatg tagatatttt tgtgaatcga aaaaaatatg caactaaaga
     1021 gattatggga ttctctcatt ttaaaacatt aaaaaatcgt tatatatttt ggggaataga
     1081 gttgattgca tcatgtatat tgactgctat aaattacaat tttgcttgtc tttttgcaat
     1141 tatacttatt gactatatat tctgtgaatt actttataga atttatatct taaacgcttt
     1201 gtatgaaata gaaaaggggg cataagcatg tcattgatta cattaaagaa cataaaaaaa
     1261 gtatatggta aaaatcaaat tttattcgag aatttgtcgt tagaaataga aaagggtgaa
     1321 tttgttggtc ttgtaggtaa aagtggtgca ggtaagacaa ctcttttaaa tataattgga
     1381 ttgattacag acatatcaga gggaataatt actattgacg gtcaacaaaa attatcagta
     1441 aacagtagaa atgcaatgct tttaagacga tatacgatag gctatctatt tcaaaactat
     1501 ggacttatag aagatgaaag cgtattatgg aatttaaaat tagctcttga atataaaaaa
     1561 atgaaaaagg aagataaact gaaagtaatc aacgaatatt taaaacaatt taatttgagc
     1621 gatatgctca ataaaaaaat atatcagtta agcggtggag agcaacagcg gattgcaatt
     1681 atcaaattaa ttttacaagg aagtcaaatt atactagccg atgaaccaac ttctggactt
     1741 gataaagaaa atgaacaggt agtgatgaaa cttctgaaag aattgaatga aaagggcatt
     1801 actataatta tggtaactca taacatggat ttgcgtaatt atttttccag agttataaat
     1861 gtagaaactt ttagatagtt tattaaaggg agatatactt ttttgtgcaa tctcccttgt
     1921 tctttttctg tcatatcaag actcgttcaa taatggtaaa ttcgtggtaa aatagctact
     1981 tttttagttg atgaatagcc cttaaatact gtatttatgc ggataatcgg attttttata
     2041 ccaagtttgg atatatcaac aaatcatttg ataattatga tcttggtttt ataattaatt
     2101 taaaaaaaat aaaaactatc gatgacttac tcacaaataa tttagttctc aaaaaagcgt
     2161 actgtgatac attctaatga cgagagcgtc tttaccggaa aaatcaatct tttagattca
     2221 aaaaaactag tactttctat aatagattac gaaagcatca caaagacgaa caaaaaaaac
     2281 aataaatata gatgcagact gtagacatca tccataaaat ggactttgtc tacagtctga
     2341 aacgagatct ttcttcttac gtgaaggaag aatatccagt aatttcccag tatcgccatc
     2401 catacaagaa aaagaatagg gaccgacttg atttttcaat gaacgaaatt catccaccaa
     2461 tgaacagcga ggcaaccaat tccttttcac gtttgtcatg gggagcagag aagcgagtgt
     2521 tcgcgaaacg gttgaccaag aaacgccgca atgtttagaa atcgtcgtca ttgcttgttt
     2581 ttcagtcaat tcctccaata ttttataatg tacttgtttg gcgataaaac agtttttttc
     2641 aacaatgtag gtattcgccg taaaataggt gtgacagcca cgacagaggt accgttgctt
     2701 tgccaaagca agataagtcc gttgattcgc acacttattg agaaggacca gcgatttttt
     2761 ctttccgctc ttaacgatca gcccatgttg cttcacctgt ttacactgag gacagcgctg
     2821 aatcgtttta atgagcgtgg cagaaacaac gatactgttg attgaatgta tttttttctt
     2881 ctcgacatga gtgatttgaa tgtttttatc tttgattcct agtaatagtt tgataagatg
     2941 aagatcaggc atacagaacg ctcctttgat tgtgttgtgg tgattcaatt ctaacggagc
     3001 ttctgtatgt tttgtttttt tatgctcaaa aggactgata gaatgatttt ctatcagtcc
     3061 tagaaattat agcgccggaa aagtttccga gcaccaaaat acccatttct cactgaagca
     3121 atgatgattg atcgattgct tgagttttct gcgcctctaa aggaggcgta tccctttttt
     3181 catgaattag tcgaag
//
LOCUS       DAPBAB010000149         3070 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPBAB010000149 DAPBAB010000000
VERSION     DAPBAB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.162"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(334..696)
                     /locus_tag="RD444_002926"
     CDS             complement(334..696)
                     /locus_tag="RD444_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550224.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKIGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="RD444_002927"
     CDS             complement(770..1150)
                     /locus_tag="RD444_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDT7550225.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="RD444_002928"
     CDS             1214..2407
                     /locus_tag="RD444_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7550226.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2484..3068)
                     /locus_tag="RD444_002929"
     CDS             complement(2484..3068)
                     /locus_tag="RD444_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7550227.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          959 a          587 c          503 g         1021 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc aatctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat
//
LOCUS       DAPBAB010000150         3011 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPBAB010000150 DAPBAB010000000
VERSION     DAPBAB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.163"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            175..357
                     /locus_tag="RD444_002930"
     CDS             175..357
                     /locus_tag="RD444_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDT7550228.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            460..744
                     /locus_tag="RD444_002931"
     CDS             460..744
                     /locus_tag="RD444_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550229.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            760..996
                     /locus_tag="RD444_002932"
     CDS             760..996
                     /locus_tag="RD444_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550230.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1018..1272
                     /locus_tag="RD444_002933"
     CDS             1018..1272
                     /locus_tag="RD444_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550231.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1354..1539
                     /locus_tag="RD444_002934"
     CDS             1354..1539
                     /locus_tag="RD444_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDT7550232.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1606..2001
                     /locus_tag="RD444_002935"
     CDS             1606..2001
                     /locus_tag="RD444_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7550233.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1991..2368
                     /locus_tag="RD444_002936"
     CDS             1991..2368
                     /locus_tag="RD444_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550234.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2390..2764
                     /locus_tag="RD444_002937"
     CDS             2390..2764
                     /locus_tag="RD444_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550235.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1059 a          507 c          523 g          922 t
ORIGIN      
        1 tatgtttatg tatatgtata tgaatataaa cgtattaggt acgaacactt ttaaattctt
       61 ttctgggctt tgtttttagg gtagggctaa aaaaaacatc accatctgat ccaaaacagc
      121 tcagaaacgc atttaaacgt atctgatacc aatattacag aaggagtgta tcgtatgatt
      181 aacctatttc ttttagtgac agctagtaca ttattcttta ctggaatatc actgataatt
      241 tcctatttga tctataagaa agccgaaaat cacacaagca aagcaatcgg actagccgtt
      301 tcatcctttt taactttgct gactttattt gttactttat cgttttcttt tgtttagaac
      361 agctgatcca ttagcacctt tttctcttta cattatacac actgttgtgt ataatgtatt
      421 tgtaaacaag ctaattaact tttaggaagg agtgacaaga tgctaacagc tactttacag
      481 caacctactc acgttgctat tgcagaaagt tatacaatta atgcagggat cgttaaacag
      541 ctgatcaagg atcgagaatt caaaaagttt aacagtcggc aagcgtataa cagacagcta
      601 aagaaagcta ttctaaagca caaagattcc ttcatcggga acttggaaag tgtagatgtt
      661 aaaactgttt caacggcatt ttcagttgtt gaagtgacta ttgacggtcg cttgatttct
      721 ctatttgctt ttggaattaa ttgataggga gtgtgaactt tggaaggtaa gacttttgca
      781 gaaatttgga ctgaatttat tgaggtacca actcgcattg atccgataac ttctgaaaag
      841 gtattggatg cagacttcta caatctcaaa gcaggtacta gccttgaatg cgtttggatg
      901 tatttagaag agcatttcaa aggcgtaacg ctcggtcacg tcttatacca caatggtgcc
      961 gagtgggatg caattggaaa tatccatttt tcttaatata agaaaggaga acacaatatg
     1021 aattataccg atgaagaaca aatcgctttg gaatacgttt tcacaaatga agaaggggag
     1081 gaaattgaaa aagtcttgat catttcatca aaagacttgg agaaggcttt ggcttatgaa
     1141 ccatacgaat caaccgtaac tgtcattgaa ttcttagata cctatacttc taatgatact
     1201 tgggaaatta tgaatatttg caacgctaat aacattcaat ttgctgaaaa aaccaataaa
     1261 tcattttatt agttttgcaa taaaacatcc tctttttcta ttgattccat acacaacaat
     1321 gtgtataata gattttgtaa agggagggta gctatgccta agacagcaag agaaattgta
     1381 aaaattctca aatctaacgg cttcaaaaag actggacaaa ctggcagtca tgcaaaattt
     1441 tataattcca agactaaaag aacggtcgtt gttccgatcc acaatggtga tgtaccacta
     1501 ggaacagaag ctaatatctg gaagcaagca gggctttaac agccttgcct tcctatttgg
     1561 tattaggcat actatcctct cttacactag aaaaggagcg aggaaatgaa gacaaaaaat
     1621 attttttatc cggcaatctt cgagaaagaa ggagaagctt ataacatcgt tttccctgac
     1681 ttaccggaaa tatctacctt tggaaatagt cttgaagaag catatttaaa cgctaaggat
     1741 gctttaggat tagcgctata tagtgtacca gatgcagaat ttcctaaacc atcagcagtg
     1801 gaagaattaa ctttgaaagc caaccaagtg gtagtaatca tccaactaaa cattaaactt
     1861 ttccgaagaa gcttaaatac aaaaacaatt agaaaaaatg ttagtgttcc ggaatggtta
     1921 gttcttttag ggaaggagaa aaacataaac ttctctcaac tacttcaaaa agcactagaa
     1981 gaagagttgc ttgaaaagta aaaaggagac cgaacctatg aaagctacac acttaaacta
     2041 tttctctcga acgttaatca ataatctatt aaaagataca actggcatta gcgttagtac
     2101 caaacacttt gccaatgatc taattatttt tgaggataat aacttgcgaa ttgagatgtt
     2161 tattgaacag gcaaccccta actcaatacc tgaattctgc tatacgcaca ctgaaaaaat
     2221 gaaacatgca aaatcagtta aagatgttga agcattaagt gaatcactaa cgaacgcttt
     2281 aaacctcgtt aagaaggcaa aattgttatt tgaatttcaa accgactacc acaatgagtt
     2341 gcttgctcaa tctgaaattt tagattaatt gaaacaggag aagagaaaga tgaaacaaac
     2401 acaaacagaa accgttaacg atacgcttgc tgatcaatta gcaggttatt ataaattatt
     2461 tgagatgggt aaagcacagc gcttgatcca ctatctttgg aaatacaaaa ttttgtttct
     2521 tttttgggaa tcaacactaa cattctcagt tcttttttat aagtcagatt tttataatca
     2581 acacttagtt ttagggagtt ttttgcttat ctacaccatt ttgatgctta atttatatac
     2641 ctacatcaca cttgaagaac acttagtaat tagcgatgca gggatcgaga gaaacatcaa
     2701 aagggtatcc ttcaagatca cgttaaacaa gcctaccgat aaaaagctct cagaagcgtt
     2761 ctgagagctt ttgacatttt cttgtataat tttatcctaa cacattacaa agcacctaat
     2821 tcgagcttat atcaagcaaa tttgctattt attactcaat acatatctaa aaattacttg
     2881 atggaaaatt agatacatgg taagcatgaa aagaaaatca atttgagcag atgcagtaca
     2941 atacatagca atttaaaagc tctcagaagg cttctgagag cttttaatat attctttgat
     3001 aatttgtctt t
//
LOCUS       DAPBAB010000151         2978 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPBAB010000151 DAPBAB010000000
VERSION     DAPBAB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.164"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            747..2624
                     /gene="ltrA"
                     /locus_tag="RD444_002938"
     CDS             747..2624
                     /gene="ltrA"
                     /locus_tag="RD444_002938"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDT7550236.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            2755..2978
                     /locus_tag="RD444_002939"
                     /pseudo
     CDS             2755..2978
                     /locus_tag="RD444_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
BASE COUNT         1130 a          466 c          647 g          735 t
ORIGIN      
        1 agtgccataa tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa gtagtctaaa
       61 aactattgta ataactctta taacgagaag tcaaatgagt gggtaacgcc acaaagggct
      121 tccactgata ctccgacatg caacaagtag tttatgcaga ccaacgttgt gtgaagctcg
      181 gtgaagtcgg ctgaaggata ccgtaaggta aaagaaatag tgaataccac gaaagttctt
      241 tagaggtaaa gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata tggtgagtat
      301 gtacaaacgt actgacgaac tagcgaatgt actgtccgaa aggcatagct cctagaaatg
      361 ggagagacag tgagcaaaaa atcactcatt gtgctaactt gtggttgagt aaaagtcgtt
      421 gttatgaaag accatgctgc gttacaggcg taggagaagt tagggggcaa aagctagcat
      481 ctaagttaag gcatgtggat agatataagg gaacagggga agccatcaac atggagagtg
      541 catactcgat gaagtggtag taagaaatat gtgaatgaca aataacttac tttagtgatg
      601 gtgaaagtag agtcacgact gatgaaggtt ctgtaatgga actggaggaa caggctcgat
      661 tcgtctgtaa aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac taaaaaggtc
      721 acgagccaat tggagcgtga taaaccatga caaaagaaac aagtccaaaa gataggaagt
      781 tgcggcatgc agaatattat ggcatgacgg aaatcttcga taagatatac tccaaaagtt
      841 tgaagggaga taatttcaag aatctaatgc agattatcat ttctgataac aacattgtcc
      901 tagcatatcg taatatcaag agaaatcacg gtagtgtgac aagaggagaa gatggagtaa
      961 cgattaaaga tattgaaaat ctcaaccgta atgaattcgt agaaattgtt aggaagcgat
     1021 tcgcacatta taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat aatggtaaat
     1081 taagaccgtt gggtattcca tcaatatggg atagaatcgc gcagcaatgc atcttacaag
     1141 tattagaacc aatatgtgaa gcaaaattca ataatcacag ctatggtttc cgcccaaata
     1201 gatcagcaga acacgcaata gcagattgta tggcccgaat gaatcaaagt cacatggaat
     1261 atgtggtaga tgtagatatc aaaggtttct tcgacgaagt caatcatact aaactcatga
     1321 gacaaatctg gtctatgggt atacgagaca aacagcttct cttaatcatt aggaaaatgt
     1381 taagagcacc cgtagtactt ccaaatggga agttgatata cccaacaaaa ggaacaccac
     1441 aaggcggaat acttagccca ttacttgcga acatcaatct caacgaattt gactggtgga
     1501 tagcaaatca gtgggaggaa aaagatgcca aagagctaaa acatcatttt aggaaaaatg
     1561 gagcttggga taaaggtaat atctatcaca agctcagaaa gagtacaact ctaaaagaaa
     1621 tatacctagt gagatacgca gatgatttta aaattttctg tagaaattat aatgaagcga
     1681 aaaaagtttt ctatgcaact aaaatgtggt tacaggagcg tctaaagtta ccaatatctg
     1741 aagaaaagtc aaaaatcaca aatctttcaa aagaagaaag tgaattttta ggctttacga
     1801 tgaaattagt gaccaaaggt aacaaaaagg ttgttaatac gcatataggt acaaaagcac
     1861 aaagcatgat taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa agtcctaata
     1921 gcaaagatac ctttatctat attggagtct ataacagtat ggttattggg attcataatt
     1981 acttcaaaat tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga gaactcagac
     2041 caagttttga aatacgacta cgagaacaag gaatctcaaa agaaggacaa tacggaaagt
     2101 acgaaggata caagccttac ctacggtcag gacaaatacg atatataaga gggtttccaa
     2161 tattaccagt agggtatatt caacacagac atccgatgtg taaaaaaaga aatattaata
     2221 aatacaccga agaaggtagg gaagccattc ataagaagca acaaaccgta gaagaatgga
     2281 agatatcgtg gttgtgtagc aatccagtgc ttagtaagag agcaacgatt gaatttgcgg
     2341 acaatcgaat ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca ggtgaggagc
     2401 ttatcttatc tgaaatgcat tgtcatcata ttatccctta tcatgaaagc aaaagtgata
     2461 gctatgaaaa cctagtgatt gttactgagg aggttcatcg ggtaatacac gctacacaaa
     2521 gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa aaagaaaaac
     2581 tcaatgagtt gagattaaaa gttggtaacg aagaaatatc ttaataaata tttgtaggac
     2641 gggaaaacag aatgtctttt atagacgagg gaacgccgta tgcggtgaaa gtcgcacgta
     2701 cggtgtggac tgggggaaaa gacggagata atatcaaact cttacctatc agtatcgatt
     2761 aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga aatgcgtaaa
     2821 gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc agaaaatatg
     2881 tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac gaagatcttt
     2941 atgcgtatgg atgaatctat tttacgtcat atagaagg
//
LOCUS       DAPBAB010000152         2923 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPBAB010000152 DAPBAB010000000
VERSION     DAPBAB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.165"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..363
                     /locus_tag="RD444_002940"
     CDS             <1..363
                     /locus_tag="RD444_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7550237.1"
                     /translation="RKKRDKQAAYAFLKRLIKQFGEPKVLVTDKAPSLSSAFNQLHSE
                     GLFSKTEHRTNKYLNNIIEQDHRSIKKRHKLYQSIRTASSTIKGIETIHALYKISQRD
                     KSLFGFSVIHEMNTLMSI"
     gene            423..899
                     /locus_tag="RD444_002941"
                     /pseudo
     CDS             423..899
                     /locus_tag="RD444_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            899..1789
                     /locus_tag="RD444_002942"
     CDS             899..1789
                     /locus_tag="RD444_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7550238.1"
                     /translation="MEELIRVEHATKTFRSKNWRGTVSEVRAVEDVSLTIHRGETLGL
                     VGESGSGKTTLGRSILNLETLTSGEIFFEGKELTALTAEDRKAAYQQMQIIFQDPYSS
                     LNPRMTALELVMEPLYHETKDAARAKAKEILAIVGITGDDVHKLPRSFSGGQRQRIGI
                     ARAVVNRPAFVLCDEPTSALDVSIQAQIIQLLLRLQKEFSLSYLFISHDLSVIRHISD
                     RTAVMYQGKLVELAPTDRLFDNPLHPYTKKLLAAAPIADPVLARGAMEQTEIQSTITL
                     TPLYEWVEVEPDHFVRREKR"
     gene            1786..2178
                     /locus_tag="RD444_002943"
     CDS             1786..2178
                     /locus_tag="RD444_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDT7550239.1"
                     /translation="MTVETLTIHQGSDKLEMTTPTGNWVLAREKGYSPVQMLVSAVAA
                     CGGYVYASVLENSKITAEIEKIEVSYDRDDNKKAQPVSAIHIQFYAKIAEADHDKATR
                     CLKLVSPNCPVIQSLDPAIEVTETVTFI"
     gene            2364..2923
                     /locus_tag="RD444_002944"
                     /pseudo
     CDS             2364..2923
                     /locus_tag="RD444_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          928 a          593 c          641 g          761 t
ORIGIN      
        1 cgaaaaaagc gtgataaaca agcagcctat gctttcttaa aacgattgat caaacaattt
       61 ggagaaccta aagttcttgt cactgataaa gccccttcac taagtagtgc gtttaaccaa
      121 ttgcattctg aaggattatt ttctaagact gaacaccgaa caaataaata cttgaataat
      181 atcattgaac aggatcatcg atccatcaag aaaaggcaca aattatatca gtccataagg
      241 actgcttcct caacaattaa aggaattgaa accatacacg ctttatataa aattagccaa
      301 agagacaaaa gtctctttgg cttttcagtg attcatgaga tgaatacatt aatgagtatc
      361 taagcataaa atcaaaaatt aattggaaac tctttattaa ttttttaact ttgcaacaga
      421 acccttttcc agtacaacgt atgaaacaat atccccacga gttatcagga gggatgcgtc
      481 agcgggtgat gatcgccatg gctttattgg cgcaaccgaa attgctgatc gcagatgaac
      541 cgacgacggc tttagatgtg acgatccaag cacagatttt ggccttgatc aaaaacctgc
      601 aaagagagaa ccagctttcc gtcgtccttg tgacccatga ctttggggtc gttgcaggaa
      661 tgtgcgactt tattaaagtg atgtatcaag gtcgagtcgt ggaagaaggc accacggaag
      721 agatctttta tcaacctgcg catccttata cgaaacaatt attgcaagcg gcacatttag
      781 gcgacaagca tgccgaatta aaagtctttg actatcatga gacattggca aatgagttgc
      841 aagaaacgca gttatctaag acccatcgcg tttggtgcaa tcaggaggtg acagaataat
      901 ggaagaactg attcgcgtag aacatgcgac gaaaaccttt cgcagcaaaa actggcgcgg
      961 cactgtcagt gaagtccgag cggtcgaaga tgtcagtcta acgatccatc ggggcgagac
     1021 attggggcta gtaggggaat ctgggagtgg gaaaaccact ttaggacgca gtattttaaa
     1081 tttagagact ttaacaagcg gagagatttt ctttgaaggc aaagagctaa cagcattgac
     1141 ggcagaagat cgcaaagcag cgtatcagca aatgcagatc attttccaag atccgtattc
     1201 atcattgaat ccccgcatga cggcacttga actggtgatg gagccgttgt atcatgaaac
     1261 gaaagatgct gcccgagcga aagccaaaga aatcttagcg atcgtcggga tcacgggtga
     1321 tgatgtccat aaactgcccc gcagtttcag cggcggacaa cggcaacgga tcggcatcgc
     1381 tcgtgcggtg gtcaatcgcc cggcgttcgt cttgtgtgat gaaccgactt cggcattaga
     1441 tgtctccatt caggctcaga tcatccaatt gctgctgcgt ttgcaaaaag agttttcatt
     1501 gtcttatttg ttcatttccc atgacttgtc cgtgattcgt catatcagtg accgaacagc
     1561 cgtcatgtat caagggaaac tagttgaatt ggcacccacc gaccgtttgt ttgacaatcc
     1621 gctgcatcct tacacgaaga aattattagc agcagcgccg atcgctgatc ctgtgttagc
     1681 aagaggtgcg atggaacaaa cggagattca aagcacgatt actttgacac cattgtatga
     1741 atgggtcgaa gttgaaccag atcattttgt gaggagagaa aaacgatgac agtagaaaca
     1801 ttgacgatcc atcaaggaag cgacaaactt gaaatgacga ccccaactgg caattgggtg
     1861 ttagcacggg aaaaaggcta ttcgccagtc caaatgctgg tgagtgcggt cgctgcttgc
     1921 ggtggctatg tatacgcatc cgtcttagag aattcaaaaa tcacagcaga aatcgaaaag
     1981 atcgaagtca gctatgaccg agacgacaac aaaaaagccc aaccagtcag tgccatccac
     2041 attcagtttt atgcgaaaat cgctgaagcc gaccatgaca aagcgacccg ttgcctaaaa
     2101 ctcgtttcac caaattgccc cgtgatccaa tcattagatc cagcgatcga agtgacggaa
     2161 acggtgacgt ttatttaatc gaaaaagact ttgtgtgcct gaaaccaata ggcgtacaaa
     2221 gtcttttggc tctataattt atgggactga tgaatcagaa ttgattcttc agccccattt
     2281 tcattttagg tattaaaaaa catatcgttc tctagtatga ttaaatcacc acaaaataaa
     2341 caactgagag gacgatatgc tatatgaatg attctatcaa aaaaatgctg agaataatag
     2401 agaaagattt gatgattaca gaggtctctt acgagaccct tcagaagaaa aagacgttgg
     2461 tcgtcgatgc tgttctctcg cctactcctc gtgcttgtag aagttgtgat tctactgtgg
     2521 tagatggaaa cgggaaagca attatggtga aaaatgggaa gaaggaaacg attgtccgtt
     2581 ttgaacaata aaatcatatg cctttggtta tgcgcctaaa aaagcagcgc tatacctgta
     2641 aaaactgtcg cacccattgg actgctcaaa gttattttgt ccaacccaga cattcaatcg
     2701 caaatcatgt tagatataaa attgcttctt tactgactga aaaagtatct ttatctttta
     2761 ttgcgaaaag ctgttaggta tctttgacca ccgttattcg tacattgaaa gagtttaaaa
     2821 gctatttacc aaagcaatct aagaagattc tccccagagt attgatggtt gatgaatttc
     2881 gttcgcatac ttctatagaa gataaaatga gctttatttg tgc
//
LOCUS       DAPBAB010000153         2882 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPBAB010000153 DAPBAB010000000
VERSION     DAPBAB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.166"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            4..2376
                     /locus_tag="RD444_002945"
     CDS             4..2376
                     /locus_tag="RD444_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550240.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..>2882
                     /locus_tag="RD444_002946"
     CDS             2437..>2882
                     /locus_tag="RD444_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550241.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIA"
BASE COUNT         1156 a          455 c          622 g          649 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga actaaacgaa caaatcgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagta gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaccccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat caaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttcttctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gttggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gc
//
LOCUS       DAPBAB010000154         2656 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPBAB010000154 DAPBAB010000000
VERSION     DAPBAB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.168"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            66..1034
                     /locus_tag="RD444_002947"
     CDS             66..1034
                     /locus_tag="RD444_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDT7550242.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1027..2058
                     /gene="vanA"
                     /locus_tag="RD444_002948"
     CDS             1027..2058
                     /gene="vanA"
                     /locus_tag="RD444_002948"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDT7550243.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2064..>2656
                     /gene="vanX"
                     /locus_tag="RD444_002949"
     CDS             2064..>2656
                     /gene="vanX"
                     /locus_tag="RD444_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HDT7550244.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYF"
BASE COUNT          778 a          515 c          665 g          698 t
ORIGIN      
        1 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
       61 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      121 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      181 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      241 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      301 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      361 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      421 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      481 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      541 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      601 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      661 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      721 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      781 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
      841 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
      901 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
      961 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1021 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1081 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1141 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1201 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1261 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1321 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1381 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1441 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1501 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1561 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1621 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1681 gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg accaaatcag gctgcagtac
     1741 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1801 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     1861 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     1921 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     1981 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2041 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2101 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2161 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2221 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2281 agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2341 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2401 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2461 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2521 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2581 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2641 ccaatagcta ttttga
//
LOCUS       DAPBAB010000155         2617 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPBAB010000155 DAPBAB010000000
VERSION     DAPBAB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.169"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            690..2552
                     /gene="ltrA"
                     /locus_tag="RD444_002950"
     CDS             690..2552
                     /gene="ltrA"
                     /locus_tag="RD444_002950"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDT7550245.1"
                     /translation="MENVSASHSIRHMEYYGMIDSFDNLYKESRDDHIFNNLMEIVTS
                     PNNILLAYRNIKGNSGSVSPGVDNKNIDDLKEIPNADFIRIVQGKFSEYKPQPVKRVE
                     IPKPNGKNRPLGIPTIWDRIVQQCLLQVLEPIMEAKFHDKNYGFRPNRSAHHALAQAV
                     RLAQLSKLTFVVDIDIEGFFDNVNHSKLIKQLWTLGIQDRWLLGVIRAMLKAPIIHKD
                     GRIEHPKKGTPQGGILSPLLANVVLNELDWWISSQWETHPTRHNYDCYQANQGYWNKG
                     NKYRALRRSKLKEIYIVRYADDFKIFCRRRSDADNIFIATKLWLKERLKLDISLEKSK
                     VVNLKKKKSEFLGFTMKLVRKRKTFVIETHMCPKAMKKVSDNLIKQIKVIQHTPTKED
                     LQKQLVKYNSMVIGIHQYYRRATHISVDCPKINQRIRIIIKNRLDVKKNGSPPSDFVK
                     KNYARSKQLRWINGYPMIPLGFVKHSHTSLPKMGICKYTPEGRVLIHKYLTFPIDIIN
                     QLMNMNNPDESIEFRDNRVSKYSAQRGRCRVTKELLELEDIYCHRIIPKNLGGKDNFQ
                     NLVIIHQNIHQLIHENDRTRASLLITKMRITGAQLEIINQFRKKAQKCELSLNL"
BASE COUNT          924 a          483 c          540 g          670 t
ORIGIN      
        1 aaaaggttag gcacacaaga cttccttgtg tagaactaat gatttgagga gtgaaatgac
       61 ggtgcaacga cctgaagcgc tccctctgat actccgattg gcaatacctt tagttagaat
      121 aggaattgtc taaagctcgg taaagtcggc tgagagttac cgtagttgca ttatgcaatc
      181 gaaagctgtt cagcggtgaa tggtgtaacc tataggtcgg gagtccagaa attactcatg
      241 gtgagaatgt gcagttgcac tgacgaactc gcgaatgtac gaatctataa gcttggggtg
      301 cagaaatgta tcttacccgc acaaatatta ctgtgacaaa gggtgtagct tgcggttaag
      361 taagaatcgt agatatgaac aaccgtgcaa tgtggtaggc attggaaagt tacaggttca
      421 gagtgagcac ctaaaagtaa atgcagggat aaaatcattg gaacgttgaa agctagtgtc
      481 gtggaggact aacttccttt gaaacggttg agacgaagaa taataagctc atttacacta
      541 gtggaagtag tggcacagta cctatgaagc agtgataata aactgtggag ggatagccac
      601 aagtcaattt actttactaa cagtaatgat ataatccata gaatcgagta tgacaaaata
      661 aacgccgacg tccgaaagga ggcggtgcta tggaaaacgt atcagcttca cattcaatcc
      721 gtcatatgga atactatggc atgattgaca gctttgataa tctatacaaa gaaagtcgcg
      781 atgatcacat tttcaataac cttatggaaa ttgtaacctc gccaaataat atcttactag
      841 cgtatagaaa tattaaagga aattctggga gtgtatcccc aggcgttgat aacaaaaata
      901 ttgatgattt aaaagaaata cctaatgcgg attttatcag aatcgtgcaa ggcaaatttt
      961 cagaatataa accacaacca gttaaaagag ttgaaatccc aaagccaaac ggaaaaaatc
     1021 gcccactcgg catcccaact atttgggata gaatcgtaca acagtgtcta ctacaagtcc
     1081 tagaacccat tatggaagca aaattccatg ataaaaatta tggttttcgt cctaaccggt
     1141 cagcacacca tgcacttgct caagcagtac gcttagcaca attgtctaaa cttacttttg
     1201 tagtagatat agatattgag ggattctttg ataatgtaaa ccattcaaaa ctcattaaac
     1261 agctatggac attaggaatt caggacaggt ggcttttagg tgttattagg gcaatgctta
     1321 aagcaccgat catccataag gatgggcgaa tcgaacaccc taagaaagga actccgcagg
     1381 gcggaattct ttccccactc ctcgcaaacg ttgtgctaaa tgaactagac tggtggatat
     1441 catcacagtg ggaaacccac cccacaagac ataattacga ttgctatcaa gctaatcagg
     1501 ggtattggaa taagggtaac aaatatcgag cgttacgtag atcaaagtta aaagagatct
     1561 atattgtgag atacgccgat gacttcaaaa ttttctgtag aagacgtagc gatgcggaca
     1621 atatatttat agctaccaaa ttatggctaa aggagcgcct gaagctcgat attagcctag
     1681 aaaaatcaaa agtggttaat ctgaagaaaa agaaatccga attcctcggt ttcactatga
     1741 aattggttcg taaaagaaag acctttgtca tagaaactca catgtgccct aaagctatga
     1801 aaaaagtatc tgataatcta ataaaacaaa taaaggtaat tcagcacacc cctaccaaag
     1861 aggatttaca aaaacaacta gtgaaataca actcgatggt gatagggatt catcaatact
     1921 atagaagggc tacccacatt agtgtggatt gcccaaaaat taaccaacgt ataaggatta
     1981 tcatcaaaaa tcgattggac gttaagaaaa acggatcacc gcctagtgat ttcgttaaaa
     2041 aaaactatgc acgttcaaaa cagttaagat ggattaatgg ctatcctatg attcctctag
     2101 gattcgttaa acacagccat acatcgttac ctaaaatggg aatttgtaag tacacgcccg
     2161 aaggacgtgt gctaatacac aaatatctta cctttccaat cgatattatc aaccaactta
     2221 tgaatatgaa taacccggat gaaagcattg aatttaggga taatcgtgtc tctaagtact
     2281 ctgcacaacg aggcaggtgc cgagtaacaa aagagctctt ggagctagaa gatatctatt
     2341 gtcaccgtat catacccaaa aatcttggtg gaaaagataa cttccaaaat ctagttatca
     2401 ttcatcaaaa cattcatcaa cttattcatg agaatgatag aacgagagct tcactcctca
     2461 taacgaagat gaggattaca ggcgctcaac ttgagataat caaccaattc cggaaaaaag
     2521 ctcagaagtg tgaattgtcg ctgaatctat aactgtatga taattgttag taaacattga
     2581 tggaacgccg tatgcggtga aagtcgcacg tacggtg
//
LOCUS       DAPBAB010000156         2572 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPBAB010000156 DAPBAB010000000
VERSION     DAPBAB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.170"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..133)
                     /locus_tag="RD444_002951"
     CDS             complement(<1..133)
                     /locus_tag="RD444_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7550246.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     "
     gene            complement(130..783)
                     /locus_tag="RD444_002952"
     CDS             complement(130..783)
                     /locus_tag="RD444_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7550247.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1071..2345
                     /locus_tag="RD444_002953"
     CDS             1071..2345
                     /locus_tag="RD444_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7550248.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            2400..>2572
                     /locus_tag="RD444_002954"
     CDS             2400..>2572
                     /locus_tag="RD444_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HDT7550249.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGI"
BASE COUNT          820 a          503 c          399 g          850 t
ORIGIN      
        1 tcttttcttt tctcgtctcc aaatatgttt taggaaagat aaaaaaaatt aatgaatgaa
       61 ctaatataat tattattcca agaacagaaa atatttgtat gaacattttt ggaaatatct
      121 ttaatttttt cattttctct ccaatttata tcctacattt cttatagtag ttatacagtc
      181 taattgtaat ttttttctaa gttcttttat atatacatct attactcgat cataaggagt
      241 ttcttcgcta tctttccata cataatcaat aatttgcatt cttgttaaca cttgtccatt
      301 attatccaat aaacatttta atacttctag ttcttttgca tttacatcta tttcttcacc
      361 atttatttta gctgtatagc tattaaaatt aaccgataga tccttatatt caaacttctc
      421 taaatgtcca taattcttct taattaaaga atctattcta gcttttaaga ccggcaatga
      481 aaatggcttt tctacatatc cgtctactaa attagtaaat gcatcaattt tatattcttc
      541 atcactaaat gcagtcaaga ttagaattgg taaattgctt ttctttctaa tttctttcaa
      601 cacttctaaa ccatttataa aaggtatctg aatgtctaag ataaccaaat ttatatcgct
      661 attaaatttt gacaaggctt ctcttccatc tttagcttga ataacagtat atccaaattc
      721 tgaaagatat tcacttattc cctctcttat catattatca tcttcaacag ttaatatttt
      781 catatatacc tccacttaag cctatacact agatattata cagcatatta tggctctata
      841 atatctagtg ttggtgacac cttctgttgc caatactttt tcttttttct aataaatgca
      901 aacagacacc ttgaaatcct ttagaataaa gggttctata atattttgtg ttgttggaaa
      961 atcgaaggcg attttctact aacacttttt cttcgtttca actcaaaaag agactcactt
     1021 ttggtaaaat ttagtcgacc aaaacatcac cctaccaaag gagtcgctct atggataatc
     1081 atactagaaa attactcgga ttaacagata aacatttatt ttttgatgaa gaatggctga
     1141 ttgaaaaaga atataaaggc gttcaagcgc agtttattaa agggagattg gatgactcac
     1201 cttcccattg tgaacactgt ggatcgattc gtattatccg caacggttcc tatacgacac
     1261 gtactcagat attgaaagtc aaagaaaagt tgactatttt agaattaaaa cgtacccgtt
     1321 ttctatgcta tgattgtggg cagacctttt ctgcgaaaac ggacttagtt gatgaacatc
     1381 atcaactaac aaaggaactg aagcaggcga ttctcataga actgtacgaa aatcaatcac
     1441 gtaaactaat cgctaagaag tacttcgtct cggatgggac ggtgacacgg atcttgcgtg
     1501 aagcaacgaa gcattaccag ccacgtatga acttccttcc gacggtgttg tgtatggatg
     1561 agttcaaatc aatgaagtct gtttctggtt ccatgagttt tatttgtgtt gatgggacaa
     1621 cccatcagct atttacgatc cttgaagatc gcagacttta caaactaact caatacttcc
     1681 tgcgctttcc tagaaaagcc cgtttaaagg tcaagtacct agtgatggac atgaatgcca
     1741 gctattgtca gttgcttaaa acagtctttc caaatgcgga gatcgtaact gaccgctttc
     1801 atattgtcca acacatcaat cgcagcttca atcaattacg tgtccagatc atgaatcgtt
     1861 ttcgaacctc acactctgaa aatcagaaga agtaccgtcg gctcaagcga tattggaaac
     1921 tcttactcaa agattccacg acattggagc cactcaaacg gcattatcat cgactgttta
     1981 aacgaccaat tagtcaaaca gaaatcgttg atgagctgtt aagctacaac gaagaattgc
     2041 gaactgcgta tcatttttgt caattattac gctattattt cgttaaacga ggtacggaag
     2101 gctttcaaga aacccttaaa acaatttcat ctacgttacc tcagtcgttt aaaaagaaat
     2161 tcacgatctt ccatcgctac caatcgggta tttcaaatgc ctttaaggtg tcccactcaa
     2221 acggcgtcac tgaggggctg aataacaaga tcaagctgat taaacgaatt gcgtttggtt
     2281 atcgcaactt ttataacttt cgggcacgga tctatttaca acaaggtctg atctttgaaa
     2341 actaaaaaaa tatgcggcgc acagaacgcg ccgcatatcg gactaagttt tattcaaatt
     2401 tgtcttcacc aacacgattt gacgaagagc caaaaattat cgtacttgat tacggtagtc
     2461 agtataacca actgatcact cgccgtattc gtgagttcgg tgttttctca gagttgatga
     2521 gccaccgcat taccgcagaa gaagtaaagg caatgaaccc taaaggaatc at
//
LOCUS       DAPBAB010000157         2526 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPBAB010000157 DAPBAB010000000
VERSION     DAPBAB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.171"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..97
                     /locus_tag="RD444_002955"
     CDS             <1..97
                     /locus_tag="RD444_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7550250.1"
                     /translation="ILGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(127..1443)
                     /locus_tag="RD444_002956"
     CDS             complement(127..1443)
                     /locus_tag="RD444_002956"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014437.1,HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7550251.1"
                     /translation="MRDDTKVDKAGLENLEDVKGTFNANGQFQVVIGPKVADIYEEVI
                     SQSGLSGANKEDVKKVAAELNLNWFQRAVKILGDVFVPILPAIVTAGLLMGLNNLLTN
                     PGIFFEQSLIEVYPNIADVADMINLIANTSFQFLPALITWSTVKYFKGNPLLGIVLGL
                     VFVHPDLMNAWNYGQAVTTGDIPHWTIFGLSISKIGLQAQVLPALVSGYVLAKTELFL
                     KKRVPETIQLLVVAPVAILFTSVITFIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFA
                     YGIGLAPLVVTGMHHAFLPVHIQLMANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKK
                     LKGVGLAAGLSAYMGITEPAMFGVNLKYKFPFVVSILTSGIIGGFMASQHVIATSVGI
                     SGIPGFLSIPFEFWPIFFICMLASCVVSFGATYLYGKNKVLLQSFK"
     gene            complement(1535..2089)
                     /locus_tag="RD444_002957"
     CDS             complement(1535..2089)
                     /locus_tag="RD444_002957"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HDT7550252.1"
                     /translation="MDVLCGKINPAGKLTITIPRHLGQIPIYYNYFNTGRPYSSEKFN
                     TTKYLDVSNEPQFPFGYGLSYTTFSMSPISIKEKGKNCWKVTTTVSNKGSRDGYEVVQ
                     LYIGAQYGRYIRPTKDLVAYKKVFVKSGDSVTVSFELTNKDFKYYDEEFNEIIDDGAY
                     WIMVGNDSSQALTNKEEIEVRINE"
BASE COUNT          843 a          525 c          401 g          757 t
ORIGIN      
        1 cattctagga ttatataaga aaacccgaaa agaaggcact ctcttcgggt tttcggtctg
       61 tactgaaatc aaggtattat tgggaatccc agcttaaatc atagataccg taagggattt
      121 tattctttat ttaaaacttt gcaacagaac cttatttttc ccgtaaaggt aagttgctcc
      181 aaatgagacg acacaacttg ctagcataca aataaagaat attggccaga attcaaacgg
      241 aatagacaag aaaccaggaa ttccactgat accaacagaa gttgcaatga cgtgttgtga
      301 cgccataaat cctccaatta ttccagaggt cagaatggaa accacgaatg ggaatttata
      361 ctttagatta accccgaaca ttgctggctc agtgataccc atataagcag acaaaccggc
      421 agccagtcca acacctttca actttttatt ttggattaag aaattcattg caatagcagc
      481 agaaccttgt gccaaacttg acatgactcc aataggccaa atataatctc caccgttcgc
      541 catcaattga atatgaactg gtaaaaacgc atgatgcatc cctgtcacga ctaatggcgc
      601 aagaccaata ccataagcaa atccgccaat aaatggaact ttttggaata accatacaat
      661 acctgcgaca atcgtattac caatagcaat tgtaatcggc gctataataa taaatgttat
      721 aacactagta aaaagaattg caactggtgc aacaaccaac agttggattg tttcaggcac
      781 acgctttttc aagaatagct ctgttttagc taacacatag ccggaaacta gagctggcaa
      841 tacttgtgct tgtagtccta tcttagagat ggacaatcca aagattgtcc aatgtggaat
      901 atctcccgtg gttactgctt gcccgtaatt ccaggcattc atcaaatctg ggtgtacaaa
      961 gactagacct aacacaatcc caagaagagg atttccttta aagtatttta cagttgacca
     1021 tgttattaac gcaggcagaa attgaaagga agtgtttgcg ataagattta tcatatcagc
     1081 gacatcagca atattaggat atacttcaat gagtgactgc tcaaaaaaga ttcctgggtt
     1141 tgttaataaa ttattcagtc ccatcagtaa accagctgta acgatcgctg gtaaaatcgg
     1201 cacaaataca tcacccaata ttttaactgc acgttgaaac caattcaaat tcaactcagc
     1261 cgctactttt ttaacgtctt ctttgtttgc cccactcagc ccagattggc ttataacctc
     1321 ttcatagata tctgctactt taggaccgat aacaacttga aactgtccat tagcattgaa
     1381 ggtacccttc acatcttcca ggttttccaa tcctgcctta tcaactttag tgtcatctcg
     1441 caaggcaaac ctcagacgtg tcacacagtg agtcagtgct gcaatattct cttttccacc
     1501 aacaaagtta atgattgatt tagcttcttc tttattactc attaattctt acctctattt
     1561 cttctttatt agttaatgct tgagatgaat cattgcctac catgatccaa taagcaccat
     1621 catctataat ttcgttgaac tcttcatcat aatacttaaa atctttgttt gtaagttcaa
     1681 aagaaacagt cacactatcc cctgacttaa caaaaacctt cttgtaggct accaagtctt
     1741 ttgttggtct tatataacga ccatattgag ctccaatata tagttgaaca acctcataac
     1801 catctctact tcctttattt gaaacggtag tagtcacctt ccaacagttc ttacccttct
     1861 ctttaatgct aattggtgac atagaaaaag tggtataact caacccatac ccaaagggaa
     1921 attgtggttc attggataca tctaagtatt tggtcgtatt aaacttttca cttgaatacg
     1981 gtctaccagt attgaaataa ttataataaa tagggatttg tcctaaatgt ctcggaattg
     2041 ttattgtaag ctttccggct gggtttattt tgccacaaag gacgtccaaa atagcattgc
     2101 cagcctctgc tcctagatgc caagcctcaa ctaatccctt acatatagat ttttaaatta
     2161 cacactagtt taaactaaaa caaacaagga aaaacttacc atttaatcaa aaaattagct
     2221 catatgaata tattagaatg taaattagac tggtcaaaat ctggtcaaaa aagaggacga
     2281 atacaggtag tcctcttttt tgttattttg atattctaaa tcatttttta ttggattatt
     2341 cccttgttcg atatagaccc aaattgatct atcaataatt atcaaactga gtcatataga
     2401 gacaaaaatt tcaaaaatga cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg
     2461 acggagtcag acacggagtc aaaacaagcc cgctgagagt aaaaaatata cagtcccgta
     2521 ggtaaa
//
LOCUS       DAPBAB010000158         2523 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPBAB010000158 DAPBAB010000000
VERSION     DAPBAB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.172"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..222
                     /locus_tag="RD444_002958"
     CDS             <1..222
                     /locus_tag="RD444_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550253.1"
                     /translation="LTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVEGKIKANQTK
                     KYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            242..1834
                     /locus_tag="RD444_002959"
     CDS             242..1834
                     /locus_tag="RD444_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550254.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1855..2190
                     /locus_tag="RD444_002960"
     CDS             1855..2190
                     /locus_tag="RD444_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550255.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2336..>2523
                     /locus_tag="RD444_002961"
     CDS             2336..>2523
                     /locus_tag="RD444_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550256.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT          997 a          395 c          525 g          606 t
ORIGIN      
        1 ttaaccgaaa tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt
       61 ggtacaaatt caactgtcta tcaagaagca aaagagcgtg tggaagggaa aataaaagcg
      121 aatcaaacga aaaagtattc tcctaagatc aagaaaccaa taaatgaatc tgttcatgcc
      181 agagtagacc cgtttgatcc aacaaagttt ttaaaaagat aaaaagtaga aggggaacat
      241 gatgaataaa tcgcctgcag tcattctcac ttgtcaattt accttgccca ataaaaaatc
      301 tttttcaaca tacattgatt atatgacaag agaaaaagcg ctggaagaaa aggaacgacg
      361 cacgccggac gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat
      421 tcctgaagga gaaacttttt cggagagtaa gacggataaa gaattcagtg aaacgtcgaa
      481 tgaagctcaa tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt
      541 taccaaatat ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac
      601 gcaaagggaa caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga
      661 gccacataaa aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt
      721 gaaaggaagt gatctgaagg agatcaaaca aatcttccaa agaggacaac aaaaaggctc
      781 aatcatttat caagatgttg tctctcacga caattcgtat cttgaaaaaa tgggcttgta
      841 cgatcccaaa acagatacct tagatgaaga aggattaaaa gcagctggaa gaaaaatgat
      901 ggagacatta ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca
      961 ccgtaacact aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa
     1021 accttacacg gaggttgaaa atggtgtgag ttatatcgta ccgaaaggta agagaagaca
     1081 agcaacatta gacgcaatga agaccaatta tgtacatgag ttagaacgtt tttcttatga
     1141 aaaggatcga atgattggta tggagaaacg aaatttattg acgcggaaaa gtgatttgcg
     1201 taacacctta acaaaagtgg taaaagagcc agcgcgttac gatcgacacg caatgaactt
     1261 attaaatgaa gtatataaaa ccctacctga caaacgttcg gaatggcatt atggggatga
     1321 aaaacgaacc aaactttcac catacacaag agagaaactc gatgagttaa ccaaatatgt
     1381 gttaacaaaa gaacctgaat atcaagaata tatacagatt tcaaaatcat tgaaagaaga
     1441 agcaaaaagt ctttatgggg aaagcaagag agaatcaaag gatgcagaaa aaaatgctct
     1501 ctttgatttg aagaagcgca caggaaatgc cattctaaca gaactgaaga aacaggatag
     1561 tcaaataaaa cctttaatag aaagtatgga tcagatgcaa tctatttaca atgaacgatc
     1621 ctttcgaacg cttaaacagt taccaacgaa tgaatacaaa tatagttacg aagaattcat
     1681 gaagcgcagg aaacaattcc aacaaagagt tgtcacgcaa cggagtttgc gtcaattaaa
     1741 tcgaacgatt aatgcccatg aagaaaaata ccacgcaagt aaagcctacg aagagcaaca
     1801 acgaagaatt caaatggaac aagaaagaca gtaaagacag taggaggaat agcaatgaaa
     1861 caatatttag atcaatggaa agttatagaa ggatcattaa gagaagaacg aatcgaacaa
     1921 cttcccgatt gtttagaaaa agagcatttg tttcagataa gagaaatgtt gagaaacgaa
     1981 cagtttgatc caaatcaatt tttagtcgtt gagtacccag ctactggtgt gtactgttgt
     2041 aatcatgtga agggtgaaaa atattttatc attcaagaat atgagggcaa gttagcgcct
     2101 tattatacta cttgggaaat gaatgaagag gggatcaata acttcccgtg taaaagcatt
     2161 gaagagtcta tttcactcac agaatgttag ttgttaagtg aataaaagaa gtaaaagagt
     2221 cctgaacgta attgtccagg actctttttt tcaatgaatt gttttccgcc aattcaatga
     2281 gtggtgcgat ttgcgactac attatatcaa aagaaagaga agggaggaag aagaaatggc
     2341 ttattcaacg attggagttg ttgttgaaaa aagcagagac aatctcgttt ttgtgacaga
     2401 aattcaaacg ggcagagctt ttgtcgtcac agacaaagcg gcaaaagcgt atcaaagtgg
     2461 cgatatttta acgttaaatc tgactacgaa gacgttcgtc gatgcggcag aagattatcc
     2521 att
//
LOCUS       DAPBAB010000159         2359 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPBAB010000159 DAPBAB010000000
VERSION     DAPBAB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.173"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..807
                     /locus_tag="RD444_002962"
     CDS             <1..807
                     /locus_tag="RD444_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550257.1"
                     /translation="KYGEVSDDYFKEKSYARFLRGEIKTITKGKYSKTSEGLYCHHIY
                     ENKYENISSLYYINCFKYPFKYQKKESLVYCDLFEHLILHALIIKETEAEYGLHGYEE
                     YLYPIAIDWFLNETDPKPEWMKKCKERAYLNQQDAEKIINKIHEIISPFKEARSAMLE
                     EEYKKSIKKNIASKLGMTVSEYEEYLVQEEKEAKKRLKLEELERLNEFNKKYPNLQKI
                     NVNNTTPRKKILNFLYELAYSKDFPKRKDFYKAKISLIRDELLEELNDIL"
     gene            complement(1244..1798)
                     /locus_tag="RD444_002963"
     CDS             complement(1244..1798)
                     /locus_tag="RD444_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7550258.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQS
                     ITKRYIGITEDEIGASLKGFKLGI"
BASE COUNT          864 a          341 c          296 g          858 t
ORIGIN      
        1 aaatacggag aggtttctga tgactatttt aaagagaaat catatgctag atttttacga
       61 ggagaaataa aaacaataac taaaggaaaa tatagtaaaa catcagaagg cttatactgt
      121 catcatatat atgaaaataa gtatgaaaat atctctagcc tatactatat taattgtttt
      181 aaatatccat ttaaatatca aaaaaaagaa agccttgttt attgtgattt atttgaacat
      241 ttgattttac acgcattaat catcaaagaa acagaagctg aatacggttt gcatggttat
      301 gaagaatatc tctatcctat agctatagat tggtttctta atgaaacaga tcctaaacca
      361 gaatggatga aaaaatgtaa agaacgtgct tatttaaatc agcaggatgc tgaaaaaatt
      421 ataaataaaa ttcacgaaat tataagtccc tttaaagaag cccgttccgc tatgttagag
      481 gaagagtaca aaaaaagcat taaaaaaaat attgcgtcta aattaggtat gactgtttcc
      541 gaatatgaag aatatttagt acaagaagaa aaggaagcaa aaaaacgact taaacttgag
      601 gaattggaaa gactaaatga attcaataaa aaatatccta atttgcaaaa gataaatgtc
      661 aataatacaa cccctcgaaa aaaaatccta aactttttat acgaattagc ttacagtaaa
      721 gactttccca aaagaaaaga cttttataaa gcaaagattt cacttattag ggatgaacta
      781 ttagaagagc taaacgatat actttaaaaa tgtttatttc tggaacttta ttagaaataa
      841 ttttgaattt ttgagtgagt ttgtttttca ttattttctc ctgataccat aataactctc
      901 aatagtttgt aggaaatact cttcaattta attactttaa taattagttc gatcaatcat
      961 tgtaatattt tattgattcg aactgtggta tttattaagt tagtttattg catggttttt
     1021 atatttcata tcacgagcta taattctatt tctattatcc tatttagatt ttgaagaact
     1081 aaaatgtttt tgttgattca tacagaccat tttgaaaatt ccaagatttt taagaagtcc
     1141 aactaactta aaaaaccttc aagaatatct tggaggtttt ttattttttt cgaaatagga
     1201 gaatcgattc aatatattaa tttttgttac aagcaggtac ttactaaata cctaatttaa
     1261 agccttttaa agaagcgcca atttcgtctt ctgttattcc aatgtatctt ttagtaatcg
     1321 attgagaact atgattaaaa ataaacatta aggtagcaat gtctctagtt ttcttgtagt
     1381 agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctctt cctaattttt
     1441 ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga ccttgttgcc
     1501 gacttggaaa taaccaatca ttttcttctt taccttcagt atataataaa atctcttctc
     1561 tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca ataataggct
     1621 tacttttacc tcgtatatct ccaactttta atcctataat atctgacata cgtaatccat
     1681 tattgatgcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt cttcttagcc
     1741 aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta tagctcacta
     1801 ttgtttccct tcttaaaaat attctgagtt tatgattatg tatatatttt accataatca
     1861 tgaacttata tatatactat gttaaaagtc aagaaaatca aggggttcag tttatgattt
     1921 tatatcattt cagtaactca gaatattccg acttttttgt acaaaaaaat taaccttact
     1981 tatatgaaga ttctaacctc ttatttaaat tttagacggg caattatccg tagaagttct
     2041 tagaatagct aatttacata attaaattat atgaaattta agtttgaaca atatactaag
     2101 aactcccctt actataaagt gaaataaatt agcatatgaa taaccgtgct accaagcttt
     2161 tcattaataa aatattttta ttgatatttt ataactttac gcaagatcat aattttgtct
     2221 gtgtagatat ttcgttatat cactaaagat aataagattt aatgacctca ttaatattgt
     2281 ttgctagatc caaactctta ttaaactttc cttgctataa aacccaaaca tagtaataaa
     2341 agaataactt attaccttt
//
LOCUS       DAPBAB010000160         2284 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPBAB010000160 DAPBAB010000000
VERSION     DAPBAB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.174"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            322..1620
                     /locus_tag="RD444_002964"
     CDS             322..1620
                     /locus_tag="RD444_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDT7550259.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTNKEIEAAKKVADQLKKDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDQFINVKEVYDQKDRG"
     gene            2133..2284
                     /locus_tag="RD444_002965"
                     /pseudo
     CDS             2133..2284
                     /locus_tag="RD444_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          814 a          389 c          404 g          677 t
ORIGIN      
        1 tttttgttga tttaccaaca tgtttagaca ttttcctcta ggaaaaaatt aactagcatc
       61 acgggtttat tttagttttt cttggaaata aaaacagcga aaatccgaac attttcacct
      121 ctctcacttt ataactttta caaaatagta cttctcataa aaagcgctct agttaaagaa
      181 aataagagaa aaatcaaaaa aaacaaaagt ttcttttaca cttgtttcta tattcgttac
      241 aatggttatg ggaagttttt caaaaggtta agaaaagctt acgggggttt ttgaaatcaa
      301 aaagtagtag gggacgataa aatgaaacat acacacaaaa tcatattggc tgtcacttct
      361 gtatgcactg ttattattct aggtgtaagt atcttttttg ttacacaagc agcaacaaac
      421 aataaattag aaaacactag tcaaacaact caacactcaa gcagcagctc aaaacctgtt
      481 gaagataaac aaacgacgaa acagcttgat caagcaaaac agcttgctgc aagttatcat
      541 tatgatgaag ccatcgcctt acttgaaaaa gacgacgcca aagaagcgca acaattgtta
      601 gcaactttga aaaaagaaaa agaatccttg gtaaaatggg aagacccgac caaaatctca
      661 cacgtcttct ttcatagtct gatcgttgat ccagctaagg cttttcatac ccagcaagca
      721 caagggtata aagattatat ggtcactatt tccgaattca ataaaaccat tgatcagtta
      781 tataaaaata actatgttct tgttaatcta aatggattag tcaaaaaagg aacagatgga
      841 aaactgacct ttacaggtgt ttctcttcca gaaggaaaga aaccgttaat tctttcgcaa
      901 gacgatgtca gctattatga atacatggac aattcaggat ttccaagtaa gttgattgtc
      961 gataaacaga accaaatcaa gaatatttat atcgacaata agaaggaaac tgttggtgac
     1021 tatgatatgg ttccattgat cgattcattc attaaaaaac atcccgattt ttcttatcaa
     1081 ggcgccaaag gaacattagc tttaacaggc tataatggtg tattaggtta ccgtacatca
     1141 aaatcagaat atggtgataa cgaaaaaaca aacaaagaaa tcgaagcagc caaaaaagtt
     1201 gctgatcaat taaagaaaga tggttggagt tttgcttctc atacttgggg acacttgaat
     1261 atgacacaag cttctttagc cgatatccaa caagataatg aacgttggca aaatgaagtt
     1321 gcacctattt taggcaaaac aaatattttg atctacccat ttggcgctga catcagcgat
     1381 tggcaacctt attctgaagc aaatcaaaaa tttgcttact tgaaacaaca aggatttgat
     1441 atcttctgca atgtcgatgc ctcaactcct gcatggggac aactaggaac tgactactac
     1501 cgtaacgcca gaatcaatat tgacggtatc cgtttcgaag ctgacctaaa aggagaaaat
     1561 ccaatacttg atcaatttat caacgtcaaa gaagtgtatg accaaaaaga tcgaggatag
     1621 aggttgtgaa agaagcgtcc agctccgagc aaataagaac tagatttcta aaatagtttc
     1681 tcatattttg gaaatctagc gcttattgct gaggagttgc ttcttgaaca ccgtgaatag
     1741 aggttgtaaa aaaaccgttt tgacctgacg ataaccagta aaacagaggt gagaccattg
     1801 ctaaaagtag cagtggtctc atctcttttt ttgcacaagt catatttgat tgtttcgtta
     1861 ttttaatcag gataaagttc ttttttttca tgctaaagag tgattctttc ttctcgattt
     1921 tcactttctt cgtttaactt ttggtaaata cttctgtcag aaaaagctct aggtagacac
     1981 acgaaagcag ataaaaagcc taacttttgc aacagtttat gctgtcgttc aaagttaggc
     2041 ttttacagtt tataaatagg gttctgttgc aaagttttcc aaaaaatcta ttttagtgta
     2101 aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc aaacaattca
     2161 aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta agctatcgtt
     2221 aagttcagga attgttatat gatcgtggaa taaatgtttg tcatactacg atttatcgtt
     2281 gggt
//
LOCUS       DAPBAB010000161         2220 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPBAB010000161 DAPBAB010000000
VERSION     DAPBAB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.176"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(51..1598)
                     /locus_tag="RD444_002966"
     CDS             complement(51..1598)
                     /locus_tag="RD444_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDT7550260.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="RD444_002967"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="RD444_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDT7550261.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..>2220)
                     /locus_tag="RD444_002968"
     CDS             complement(2043..>2220)
                     /locus_tag="RD444_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550262.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          622 a          465 c          397 g          736 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
//
LOCUS       DAPBAB010000162         2213 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPBAB010000162 DAPBAB010000000
VERSION     DAPBAB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.177"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(141..1433)
                     /locus_tag="RD444_002969"
     CDS             complement(141..1433)
                     /locus_tag="RD444_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7550263.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVHTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1707..1967)
                     /locus_tag="RD444_002970"
     CDS             complement(1707..1967)
                     /locus_tag="RD444_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550264.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          736 a          416 c          346 g          715 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaatagtta agagttttta accaccataa
      121 ttctctcggc tattttgaaa tcagataaat tttgatcgga cacatagttg aataaaccta
      181 ccgttgttac ggaaggtaaa tcgcatactt ttcattctag aggagaaggt ctcgttgatc
      241 attaactgat taagcaccat agcccagttt tgaattcgac cacctgacca cttcgcgtgt
      301 aattctttcg tgcgtaaata aagtagtttt aggagtgcat tctcattaga gaacgctcct
      361 ttttttgtga cttttctgaa gctggagtgg acactttcaa ccgcattggt agtgtacata
      421 atttttcgaa tggcactacc ataatcaaat agttgttcaa catgtgcaaa gttccgtttc
      481 cagacatcta cagcaccaga ataatgagac caccgatttt gaaaactgtc aaaagcagca
      541 tgtgcagcgt ttagagaaga agcaccgtag aactttttca tgtctcgaca gacttcctta
      601 tagtccttac ttggaatata gcgcaatgca tttcgaacaa gatgaacaat acagcgctga
      661 acaattaccg atggaaagat cgcttttgcg ccttcttcga ggccagaaac accgtccatc
      721 gaaatgaaaa agacatcttc gacaccacgt gctttcagtt catcaaatac ttgcatccag
      781 cgatttttag attctgtttg atttaaccat aatcctaaaa tctccttatt tcctttgaga
      841 tcatagccaa gaatggtgtg tacagcatat tctttggctt cataattttc tcgtaaagta
      901 acatacatac aatcaacgaa tagaaaggca taacactttg ctagggggcg ggcttgccat
      961 tcttccaatt caggaaggac agcgtcagtg atatctgaaa tcatttcatg ggaaatatca
     1021 aagccataaa tagcttcgac ggttgcggca atatctcgtt gactcattcc tcgtgcatac
     1081 atggaaagaa ccttcccttc gatgtcggag acatctcgtt ttctcttagg aattaactct
     1141 ggttcaaagg aagcttcccg gtctctagga acatcaatag ctacttcacc aaaactggtt
     1201 ttaagcgttt tagttccata gccatttcga cggttatcgt gttccttagg ctctttagaa
     1261 tgggcatcat aacctaaatg attattcaat tctccttgaa gcattttttc aaaaaggggc
     1321 ccaaacacat ctttcaaagc atcttgcatg tcatcgacag attcaggttg ataggcattc
     1381 agaatggatt cagctaactt ttcggcatca ggatttcttt tctttctagc catcgtgtaa
     1441 tcacctcttg atcttattgt agaaaaagaa aaaccgcgaa gcaaccacct gcctaggatt
     1501 aatggttact tacacggttt atattacact ctcatcatta aaaaagtatc tttcgatatt
     1561 actaaacaat gaaattatct atttgaaatt taaatctgat aatatattga gtgttccaat
     1621 gagggctttg atcctattat ccatatcttt ttcagtttta atattgatac acattataag
     1681 aaaaagaaac ttttggttaa tttatttcat cccagtgcaa aatataaaaa agtaaaatag
     1741 accataggca atagattaac aattaatata atacaagcaa atattctatt cattgtattt
     1801 ctgctttcaa aaagttctaa tattgaaagt ataattccac aaatgcctat aataagtatg
     1861 ggaataagat tagaaaataa caccccgagc caatctgtga aaattactat atttactaat
     1921 atccctatta acatacctat catatttatt aaatcaattt ttttcattag atatttctcc
     1981 tttattaaat ctgaagtact tatattaaaa taattctaaa ctatttacct caaaaagtgc
     2041 aataaattat ttctgaacaa taaaatgata gtcaaaacaa aaaaatattg ttaatcgttc
     2101 agagccatcc gagagtgtaa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
     2161 gtcgcttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga ctt
//
LOCUS       DAPBAB010000163         2189 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPBAB010000163 DAPBAB010000000
VERSION     DAPBAB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.178"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(325..528)
                     /locus_tag="RD444_002971"
     CDS             complement(325..528)
                     /locus_tag="RD444_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550265.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="RD444_002972"
     CDS             complement(987..1952)
                     /locus_tag="RD444_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDT7550266.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAPBAB010000164         2179 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPBAB010000164 DAPBAB010000000
VERSION     DAPBAB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.179"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            134..307
                     /locus_tag="RD444_002973"
     CDS             134..307
                     /locus_tag="RD444_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550267.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="RD444_002974"
     CDS             947..1420
                     /locus_tag="RD444_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDT7550268.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="RD444_002975"
     CDS             1429..1656
                     /locus_tag="RD444_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7550269.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          707 a          345 c          321 g          806 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     1801 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     1861 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     1921 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1981 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     2041 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     2101 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     2161 cattattttg ataataatg
//
LOCUS       DAPBAB010000165         2083 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPBAB010000165 DAPBAB010000000
VERSION     DAPBAB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.180"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..317)
                     /locus_tag="RD444_002976"
     CDS             complement(<1..317)
                     /locus_tag="RD444_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDT7550270.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKT"
     gene            970..1065
                     /locus_tag="RD444_002977"
     CDS             970..1065
                     /locus_tag="RD444_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDT7550271.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1368..1613)
                     /locus_tag="RD444_002978"
     CDS             complement(1368..1613)
                     /locus_tag="RD444_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550272.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          677 a          380 c          344 g          682 t
ORIGIN      
        1 tttgttttat tgatttcaaa accagtaaac aaaacaaagt agcgaataga atcctcatct
       61 tcagtacggt aagtaagatt aagcatatct ttatagagct tttctaaatc accgacaaag
      121 gcttttgtag cagtcatctt ataatcactt aactctttta attcctcgaa agaaaatcta
      181 acttttttca aaccacgatc tttcatacga gcgcataatg caaaaaataa atccatctgc
      241 ttagcattga aatttttcag cggtactaaa ttcaattcat tttgatattt gactgttaat
      301 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
      361 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      421 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      481 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
      541 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
      601 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
      661 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
      721 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
      781 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
      841 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      901 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      961 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
     1021 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
     1081 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
     1141 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
     1201 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
     1261 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt ttcattggtc
     1321 caagctaaaa aactcttaga agcgatttta gagccattta aacccactta attatattct
     1381 ccatgaaatt cgctccatgc gtcaaaagct gcgtctgaaa ttccgccgtc ttcgttcact
     1441 agttgcatta atttttccaa cgatcgtttt ctttcgggtg tcatattttt atattccaac
     1501 aatttttcgt ctgttaaatc gaacttgatt gcttgaattg cttgctctaa tttttctaag
     1561 ttttgaaaat attcgtaagg aaatttttcc attttttctt tctccagtct cacaattttt
     1621 ctagaaataa aatcacttct ttgccgtcta tactctcaaa aaaaatgtcg tttgaaaaac
     1681 taaataaatt tttgatcgtg catttttaaa tttggttatc gattacacgt gcagcactcc
     1741 cccccgaatt ttaaagagca taaggaaacg tgggaacact ttttaaaaaa agtgttccaa
     1801 taggctgtgc ctattacgtt tctcaaaaat aaatgtggga acaaaatatt taaaaatagg
     1861 atagtgaaag gatagtgaag gatagtaaaa ggagggtagt aggatagcaa aaaggatagg
     1921 ctccttttcg ctatcctttt taaattggag gaattaatat ggaagtaaga ataagaaatg
     1981 tggatgagaa aatcgtaaga gaaattgatc gaagatgtga cgaacggttc tgttgcaaag
     2041 ttttaaatct actatcaaat aaggtagaat aatagaaaaa gat
//
LOCUS       DAPBAB010000166         2031 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPBAB010000166 DAPBAB010000000
VERSION     DAPBAB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.181"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..452
                     /locus_tag="RD444_002979"
     CDS             <1..452
                     /locus_tag="RD444_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550273.1"
                     /translation="AELLRLETEKYIMNVYLERSELVENFVSYMICRKHYYSESEYYS
                     IFESMELMEELNSKDATVDDYYLVFTKFLTCGTESIVLMGMDYELAVPEVFLREYVEE
                     VDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQRFERKKGQ"
     gene            833..1012
                     /locus_tag="RD444_002980"
                     /pseudo
     CDS             833..1012
                     /locus_tag="RD444_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1074..1258
                     /locus_tag="RD444_002981"
                     /pseudo
     CDS             1074..1258
                     /locus_tag="RD444_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            1292..1723
                     /locus_tag="RD444_002982"
     CDS             1292..1723
                     /locus_tag="RD444_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7550274.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            complement(1897..>2031)
                     /locus_tag="RD444_002983"
     CDS             complement(1897..>2031)
                     /locus_tag="RD444_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7550275.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          690 a          316 c          421 g          604 t
ORIGIN      
        1 gagcggagct tctacgacta gaaacagaaa aatatatcat gaatgtttac ctagaaagga
       61 gtgaactggt cgaaaacttt gtcagctaca tgatttgcag gaaacactat tactcagaat
      121 cagaatatta ttcaattttt gagagcatgg aattaatgga agagctaaac agcaaagatg
      181 cgacggtaga cgattattac cttgtattta ctaaattttt gacgtgtggg acggaatcta
      241 tcgtgttaat gggaatggat tatgaactcg ctgtacccga agtatttttg agggaatatg
      301 tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat tttttagata
      361 cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat
      421 atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt gagaatgtgt
      481 gaaataagaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc
      541 caaccgcccc cgccgcttag gtggtgcgaa ataagcgagc aaagcccgaa aacctaagag
      601 aaagaaggag atttatatga acaaaattat tcccttatta ttagtcctat ttgttggaga
      661 aatacttatg cgacttagtg cagctgtaca tttaaacctc gcagtatttt tggtaatact
      721 aatgttttgt ttgatctttt ggcgatttgt attagccctc gtaagtctac tattcaagat
      781 gattgcagcc atatttttca taggattatt tcgatgaaag gagagagtac caatgccaat
      841 tgcaatagga aataaacggt tacccgtgac gttggatgaa aagagacaaa aagaattgca
      901 gtaaccaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt
      961 attgattgcg caagaaaaag caggatttga cataccagca ctcaaaaagt gatcgcactt
     1021 tgcgcacgtg ttaatacgtg ttataattat tttgtaagga ggtatagtgc acaatgccac
     1081 ttacgggaaa agaaatgctt aagttattga aaaaaatggc tgggttgagc gaaggcaaga
     1141 gggatttcat catcatcttt ataaggatgg tgttagaatc actgttcctg ttcatgccaa
     1201 tcaagatctt ggccgaggac ttgagcgaaa aattttaaag gatgcgggat tgaaataatc
     1261 tcgcattcga ttaaagaaag gattgactga aatgttaagt gagcctttag caaaaaccta
     1321 tcctgctatt tttagcccag aagaaggtgg tgggtacttt attgaatttc ctgatgttca
     1381 aggtgcttac acaggaatta acgaagacga tatatcttat ggaattgcta tggcacaaga
     1441 ggtgttaggt atggttttag cagattatat tgagcatgaa gatctacttc cagaaccaac
     1501 accaatcaat aagatttctg ttgaagatga ttcttttact actttaattc gtgtagatgt
     1561 tgccaaatat ttgaaagaca cagaacttgt caaaaaaacc ttaacaattc ctcaatgggc
     1621 ggataagcta ggcaaaaggg ctggcataaa tttttcagtc cttctaacag aatcaattgc
     1681 agataaagca gataacattc ttcgttctgg aagaaataat tagaactcaa aagggtcaga
     1741 aacttaaaaa tctttggctt ctttttttac tacaaagatg gcttctttaa attatttgcc
     1801 tgattgattt aaaaaaattg cgcaaagact acgagctaat taagttgctc gtggtctttt
     1861 tttatgaaaa ctttttagat cttattattt gtcaaattaa gcgagacaaa actttccatt
     1921 tacgtaactc aaaaaaactt ctaatggtgt ttggtaattt agtgattttc tagggatatg
     1981 gtttcttttg gacgcaacgg atgagatgaa tccttgattg acttggttga a
//
LOCUS       DAPBAB010000167         2001 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPBAB010000167 DAPBAB010000000
VERSION     DAPBAB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.182"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            192..290
                     /locus_tag="RD444_002984"
                     /pseudo
     CDS             192..290
                     /locus_tag="RD444_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(513..743)
                     /locus_tag="RD444_002985"
     CDS             complement(513..743)
                     /locus_tag="RD444_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDT7550276.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(973..1791)
                     /locus_tag="RD444_002986"
     CDS             complement(973..1791)
                     /locus_tag="RD444_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7550277.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT          705 a          315 c          295 g          686 t
ORIGIN      
        1 accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt tactgataga
       61 cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat tttggcaatt
      121 tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat tcaaacatta
      181 gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga acacctttgg
      241 aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga caattaagag
      301 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
      361 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      421 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      481 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      541 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      601 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      661 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      721 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      781 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      841 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      901 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      961 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
     1021 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
     1081 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
     1141 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
     1201 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
     1261 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1321 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1381 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1441 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1501 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1561 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1621 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1681 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1741 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1801 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1861 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttattatcca
     1921 aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt tagatcttat
     1981 ttcaaaaggt ttgattgttt c
//
LOCUS       DAPBAB010000168         1995 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPBAB010000168 DAPBAB010000000
VERSION     DAPBAB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.183"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..741)
                     /locus_tag="RD444_002987"
     CDS             complement(<1..741)
                     /locus_tag="RD444_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDT7550278.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGICYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEA"
     gene            complement(745..1803)
                     /locus_tag="RD444_002988"
     CDS             complement(745..1803)
                     /locus_tag="RD444_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDT7550279.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT          641 a          425 c          328 g          601 t
ORIGIN      
        1 agcttcatta tcttgaagct ctccacgggc tgtattgatc aagatgctgc cttttttcat
       61 ttgagctaaa aatccttcat tgatcatttg atcgttttca cctgggatat gaggtacatg
      121 aatactgacg atatcgcttt tggctaataa ctcttcaagc gagtggaacg tcaatacttt
      181 tttagcagct tctgtctgat gaatatcgta gccgataact ttggctccta gtcccttaaa
      241 caaaacagct tccgtcaacc ctatcctgcc tgttcctatg atccctactg tgcaatttct
      301 gatttcacga ctaaacattt gctcatccac tcgaaaatct ttgaaagatg ttcggatcgt
      361 cgtatacgca gtatttctca aaagcatcat ggcaaaagtc aaagataact ctgctatcgc
      421 atttggcgaa tacgatggta cacgggctac tgtcatacca aaatcagctg ccgcttgtag
      481 atcgatatga ttgactccca cagtccgtgt aaaaacataa caaatcccat attcctgcat
      541 ttttgcaaga ttggtctcat ctgctataca atttcccctc aacaatacag catcatggtc
      601 ctttgctgac acgatattgt catgtgtcaa cagttcttca taaagactga gatcataatg
      661 gtaaatattc aatttttcaa aaaaatctat ctcgttcgga cgaacaccgt aacatgcgat
      721 tctcatactt tcttcctcca tttcttatga aaataaacct atgccatgaa tcatttgtaa
      781 tacaacgatc catacaggtg tcagtaccac agcaaaaata gttgacagta aggatgcatt
      841 ggaggctaaa agtgcctccc tatcaaaact gatagcatag gctgctgcca ctgttgcagc
      901 tggtgtagcc atcatgataa cgattgttgc cagtgcttca aaattgactg gtagaatatt
      961 cgttgccgtc aaaactgcta gaatcacgat attcaatgca ggtacaaata ccactttgac
     1021 tagactgtaa taccatgaag ttttttcaga tgcagctttt ttgaagctta cttcacctaa
     1081 tgttgcgccg attgctagcc acgccaatgg tgaacttaaa ccagctaaat aatccatcgg
     1141 ctgaaatagc cacggtgcag tctggtcaat tcgcaaaaac gctacttggc ttatctctcc
     1201 ttctgcacct gtaactgaca cttgtggcag atacccttgg aacagccaaa tgaaaaggcc
     1261 ggcaaaagta gcgataatga ttggattgac gaacatcgct ttgatatttt tagcttccat
     1321 cttcaagcca ctcattttta tgtatccata agaatacaaa aagattcggt agccaatatt
     1381 gaaaattgag gaatacatca cgccgacagg accataaatc gcactgacga taggcgttcc
     1441 gaaaaaggtc gtagaaccaa agatcgtcag gacacgcaac gttgtttgct tatccccttt
     1501 atagcttaag aaaaaaggtt ttgaaactag aataagaata atataaataa ctaatcccca
     1561 aatcaagaca ttgattcctt gcatcagtaa ttctgtattg atatcttgca tgaaagctcg
     1621 aaatgccagt gctggcaaag ccactgttaa gacgactttc gatagaattt ttccaacaga
     1681 ttcagaaaaa atcccctttt ttctacaaac aaaccctaat aatataataa aaactgttga
     1741 agtgattgca ctgacaatcg acatatctgt caaagtcgat cgaaaaatgt caaataagtt
     1801 catctttctc ctcctaatta tgttcattta ttcacaataa aaaaacgctt ttattttgct
     1861 aatacgtatt aacattttta attttatctt tacattgtta cttattgaac aataggaaat
     1921 taaagaaact tttgaaactt atgtaaagtg atttttacta gaacacaaaa aaaaccttaa
     1981 aaccaaactt aagaa
//
LOCUS       DAPBAB010000169         1994 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPBAB010000169 DAPBAB010000000
VERSION     DAPBAB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.184"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            127..1851
                     /locus_tag="RD444_002989"
     CDS             127..1851
                     /locus_tag="RD444_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7550280.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          783 a          241 c          348 g          622 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaattat
       61 atcttcttaa acaatgataa tattaaaatg caagcgcttg taaaaaataa ggagaagagg
      121 taaaaaatga agaaaaaact attaacttta tttcttgtga tgtctttctc aatcgttttg
      181 agtgtgtact acaatactat tgttttttca gaacaaataa actacacaga agaacagtat
      241 aatatagaaa aagaaaaagc tttaaaagtt ggttatagtg aagaacagtt taagcagatt
      301 atggaaatac ctacaaattt atcaaatgaa aatagtgaaa caagaatagt aaattatact
      361 atgacttcta atcaaaccaa agtcattaat aaagcaatgg agcaaattgg aaagccatac
      421 gaatggggag catcgggacc aaattctttt gactgtggtg gtttagtgaa atatgtttac
      481 aaacaagctg taaatattga actgcctatg ggaaccacca atcaagagca atacggtaca
      541 gaggtaagtt taaattcact taagcctggt gatttattat tttacggcaa tcgaggtgca
      601 acttatcatg taggtatata taaaggtaac ggtgtaatga ttcatgcacc gcaacctgga
      661 gaaactgtaa aagaggttaa catacaatat ttttatccta actttgcaaa aagaattttg
      721 cctgatgaac ctgactaccc ttatattgac tataataaaa tggtaactgt tacaaaagct
      781 tggtcaatat ggaatgattt acaatttagt catgaaatta agaaagctat tattggagat
      841 aattacaaga tagggaaggt ttatactaat ccagaaaata ataataaata tggagaaata
      901 ttagtaagta ataaagtata tggatacatt aatttttatg ctgttaaaga gttgacctct
      961 gttcaaataa acagatattt aacttctaaa gatagtggtc aaccaacttg gggaaatcta
     1021 gagtgtacta taagtaaagg acaaactaca aaagataaaa tatattttgt taaaggagca
     1081 tataatttag gggatggaaa gtatctatat tctatttata aagatcagga ttcctctgaa
     1141 tggttagggt atttaaaagc acatgttagt ttagcctata ctcctattga agaaattaat
     1201 aaaaacgtaa ctgttactaa aaattggtcg atttggaata atctacaatg ggaaaaggaa
     1261 attgaaaagc ctcagattgg ttctgtattt tcagctagat taaagttaac caatgtatcg
     1321 aataatgctg tctattacaa gctttataaa tctggaaaat tttatggtta tatcaatgct
     1381 gaggctgtca aagaccttac aacaacaaag cttaataagt atgttacatt tagtgttaat
     1441 aatgaagatt tttggtcttc tttagatgta aattattcaa aaggaaaaac tgaaagaggc
     1501 cgagtatttt acattagtat ttcatataat accgcagata accaaccgat ttattcagta
     1561 tatacagatg aaacatgtac ggaatggcga gggtattata agggaaacaa ctttgaagac
     1621 actcaaatta ctatgcttga gaataaaagc gtgaaagtaa caaaaagtgg gtatacagta
     1681 tggggagact tgaatttttg gactaagtca ggaatatcga acacaggaga tatatacacc
     1741 actgaccgaa aattttataa ttttaccaac aatgcttact attatgaatt gaaaaaagat
     1801 gggagggtct atggctatat aaatagtgaa gcagctgtag agatgaacta aaatattgct
     1861 gtcccttaat cttatggggc agcaatattt ttatattatt aatcattttt ctgaagcgag
     1921 ggtagaagaa ttaatcaata gtttttcttg ttttgtaata taatagttta attaccaaaa
     1981 attatttaag taaa
//
LOCUS       DAPBAB010000170         1940 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPBAB010000170 DAPBAB010000000
VERSION     DAPBAB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.185"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..1875
                     /locus_tag="RD444_002990"
     CDS             <1..1875
                     /locus_tag="RD444_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550281.1"
                     /translation="GSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKT
                     ANINGIITPDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYV
                     YTSGYRRYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETG
                     QRMKSAIEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIA
                     LFKGSQSVTKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLND
                     IRKNGFGNRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNE
                     RGLKGTAEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDA
                     YGEGQVNAAVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKIGLPKAQNLP
                     MKDSRLNQFKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQN
                     VVTREAAPEPIQRVHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP
                     "
BASE COUNT          730 a          311 c          397 g          502 t
ORIGIN      
        1 ggttcacctg cttttcaaat tattcaaaat aatgtgattg atgtgagtac gttactagaa
       61 aatgatccga acaaagtaga acacatacca acagacaaaa gaaatgctct cacggcgaaa
      121 aattttaaaa cagcgaatat caatgggatt attacacctg atcaagctaa gaaaatgtct
      181 gaatcaagtg atattagtac agtacaaaaa gatcggttaa aggcattaca gtatcgacta
      241 gagttagacg ataatgggaa agaaatccct gttcaaatta gtgatgaagg tttagcaaaa
      301 tatgtgtata cttcaggcta tcgccgatac attagtagtc caggtattat tttagctggt
      361 gaatcttctt tgtcggtagc ctatttattt atcttgttta cgattgttac ttgcattatt
      421 gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt tgagacagga
      481 caacggatga aaagtgctat agaagatatt tctcaatcct tcttgttatt ggcatttacg
      541 attttagaat tacgaatcta tacattaatg ctttcaggta tcggtgattt gcatgcggca
      601 ggaaagatca atggattctt gtatgttgtc gctttaattg tattaaccat tgcgctattc
      661 aaaggctctc aatcagttac gaaaattttc ggcgttgata cgagtctgaa aaacggcagc
      721 aattctcttc ttagcatgtt tgccttagga aatgttgcga aaaatgtggg tagcggggct
      781 aaaaaccttg caagtgctgg taagagtggt ttaagcaatt taaatgatat tcgtaaaaat
      841 ggatttggta atcgaaaaga tgttacccct gataacgggg aagaacaatc agacgataaa
      901 gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa agcgaaaaat
      961 ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca
     1021 gctgaagatc ttacagaaaa agccaaagac accttagaag agaaattaga cggcacgaga
     1081 gctaaagaat tggctgaagc tgtgaagaat ccatccgaaa cactagaaaa acaaaaagaa
     1141 aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca
     1201 gcagtaaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtgcagc gattccttca
     1261 gccaaagatc accaagatga gttgcattct aacgaaaatt cagaagaagg acaaaatggt
     1321 caattaccaa aaacagatcc agaacaatat cgttataaat ctaacattaa ttctgaaaat
     1381 gaaaagcgca tttcagattt accaaaacga aatcaagaag tacaaacgga tgatgattcc
     1441 atcgatctgc caaatgataa tggggtcgaa gttggaactg aaaaatcaac gaaaattggt
     1501 ttacctaagg cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaggagaat
     1561 gatcaaagct ttgttcacca atcaactgca ggtaatgttc aaatgaaacc aagtcgtttg
     1621 cctgaaaatg gaaatctgac aactgtttct tacaatttat ctgaaagtgg ggagacaggg
     1681 aatgcgtccg ttcaaaatgt tgtgacaaga gaagctgcac cagaacctat tcaaagagtt
     1741 cacacaggtt acaaaccagc aaatgtggca tttactgcac caacaaaaat aaatgtgaca
     1801 agagaaacaa aaaacaggaa caatgcattg aaagaacaac tattgaggga tatcaaacag
     1861 gatatcaaac cataaaaggc ctacaagtta ggcctttttt ctatgtatga aaaggagaaa
     1921 tcatttatgg ataaatatag
//
LOCUS       DAPBAB010000171         1907 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPBAB010000171 DAPBAB010000000
VERSION     DAPBAB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.186"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(289..362)
                     /locus_tag="RD444_002991"
     tRNA            complement(289..362)
                     /locus_tag="RD444_002991"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Ile,seq:cat)
     gene            complement(551..1570)
                     /locus_tag="RD444_002992"
     CDS             complement(551..1570)
                     /locus_tag="RD444_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDT7550282.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1581..1787)
                     /locus_tag="RD444_002993"
     CDS             complement(1581..1787)
                     /locus_tag="RD444_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDT7550283.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          562 a          389 c          324 g          632 t
ORIGIN      
        1 atataaaaga gtttaaagcg taacacactt atggggaagt ggcttggggt gcgctttaaa
       61 ctcttcttta ttatcatctc acaatttaac ccaaatgtct ttctatttaa aaatcaaagt
      121 aaaacttttc aaatatacag aaatataact atgtgaaaca tcctttcatt aatccataaa
      181 aggatacata aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa
      241 tactcttcaa aaaattctaa cacagaacga ttcaaatggc tacgttaatg taccctgtag
      301 gactcgaacc tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt
      361 actggttgtt gccacataaa gccataaaca atcaaccagt agaatgtgtg gcaacaaacc
      421 tgttatcgca tagcttggag tgtgactatt atgggtgata gtgaagatat gcgataacat
      481 cactatttta tcgaatgatt tttatagttg tcaatatagt tatgtaatgc tcctcaacga
      541 ggagctattt ttatcgttta ggaatattta aataccaacg tttgtcatgg aaatcttgcg
      601 caccgccttt agtgtttccc tctggatcat tcgttgcccg catcatgaca tagactttct
      661 tattagggaa gttacgcatg ttaaaagata catgatagcc aacatttcca gaagtattat
      721 aagcttgatt tacatctgat ctataaattc catcagctct tactcgagct aattctttcc
      781 cagtattgta atccataatg aagatatact cgtatttata gttagcaatg tgccatccag
      841 ctacatgcaa atttgcattt tcgatttctc caaactgatc aatgtgggcg taattcgttc
      901 catctgtcag tgtaggatta gctgcaccag ctcgtgttgg atcaatgaca ggcttgtttt
      961 cagaagttgt tggattttca tcggtaaatc catgagctaa atcatatgct aatttttctt
     1021 tacttacgcc catttcagaa agataaccgt aaggatctgt atgatcgccc cagatgtttt
     1081 gtgttaccca taaatgcgat ttgattcccg gttggttata aggagtgtcc aacgttaatg
     1141 gaataccata tttcattgct gaatctctag ccaattcaac gtatgccttg tagtttttct
     1201 caaacgttgc tttatcatgt gtgtgttgta actcaatctg cacaggactg ttggcattag
     1261 catacgaacc agcaccgtac tgtacataac caggttgacc gacttgataa acaattccgc
     1321 cgtctcccac aatataagca gtataagcgc tagtccatga acgttgcata tactgcgctt
     1381 cattgcgtcc tgttgctgtt tcattagccg tttcatgcag taaaatatac tgattatttg
     1441 ctacttgtga gctaccttca tttgcgccca aattaaattc attgttgata gtataggcaa
     1501 atccgttaat tggcaataaa aaaagagccg ttaacaggct catcgcagta atagtaattt
     1561 tctttttcat atctatcctc ctatttatct gatgaaattt tcttatacgt tcgatttgat
     1621 acacctaaaa tactccctag aaatgctcca aaacctgtaa taatcatcac cgttatatct
     1681 gtgtaccgcc agttaacacc ttttccgaca agaccgacaa aagtcgctaa agcggggata
     1741 actactatag cgatccattt caaaatatca aaggttctat tgttcatact atccacctcc
     1801 cttcattggt tgggctactt tctttttcaa tcgcttgttt tcttctatca atatttctct
     1861 ttctgaaagc aaggcatcaa tttgcccgtc cagttgttca attttat
//
LOCUS       DAPBAB010000172         1750 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPBAB010000172 DAPBAB010000000
VERSION     DAPBAB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.188"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(430..1002)
                     /locus_tag="RD444_002994"
     CDS             complement(430..1002)
                     /locus_tag="RD444_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550284.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1018..1623)
                     /locus_tag="RD444_002995"
     CDS             complement(1018..1623)
                     /locus_tag="RD444_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDT7550285.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          514 a          357 c          257 g          622 t
ORIGIN      
        1 taatatcaca ataggaatgc tgtttattat attagcagca gtgctgattt ttatgatagt
       61 tatttggttc ttaaatgaaa gatctaaaga caatcaacac cgtagggata tggagataaa
      121 atcaatagag tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac
      181 gtgacttacg ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata
      241 tatgatggta tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta
      301 cagttaccat acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac
      361 aacatgctaa tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag
      421 acttcttttt taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa
      481 accggttata gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg
      541 cctcttcttc ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt
      601 tcttgcaaac atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc
      661 tacactcaac aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag
      721 attcaaaacc ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc
      781 tctcgtgtta cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag
      841 accagccaac agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga
      901 aaaaatattc ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact
      961 ttgagaagcg gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta
     1021 caaatcatct atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt
     1081 catatataac tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat
     1141 ttccaaatcg tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa
     1201 gttccaatct acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt
     1261 acttcggcca tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat
     1321 aatctcttca aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata
     1381 cataactggt gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc
     1441 aaaatcgtaa atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc
     1501 aacttcgatt tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag
     1561 tttttcttca accttagcca actcaacttg attggttaat cccaatttat tctctaaaat
     1621 catgcgaaat ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata
     1681 tcgcgatttt gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa
     1741 gtgtctctat
//
LOCUS       DAPBAB010000173         1696 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPBAB010000173 DAPBAB010000000
VERSION     DAPBAB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.189"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..620
                     /gene="vanR"
                     /locus_tag="RD444_002996"
     CDS             <1..620
                     /gene="vanR"
                     /locus_tag="RD444_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDT7550286.1"
                     /translation="YTVFKYYTAKEALECIDKSEIDLAILDIMLPGTSGLTICQKIRD
                     KHTYPIIMLTGKDTEVDKITGLTIGADDYITKPFRPLELIARVKAQLRRYKKFSGVKE
                     QNENVIVHSGLVINVNTHECYLNEKQLSLTPTEFSILRILCENKGNVVSSELLFHEIW
                     GDEYFSKSNNTITVHIRHLREKMNDTIDNPKYIKTVWGVGYKIEK"
     gene            598..>1696
                     /locus_tag="RD444_002997"
     CDS             598..>1696
                     /locus_tag="RD444_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7550287.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYT"
BASE COUNT          552 a          340 c          371 g          433 t
ORIGIN      
        1 attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata gacaagtctg
       61 agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt actatctgtc
      121 aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa gatacagagg
      181 tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag ccctttcgcc
      241 cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag
      301 taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat gttaacaccc
      361 atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt tcaatactgc
      421 gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt catgagatat
      481 ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc cggcatttgc
      541 gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta tggggggttg
      601 gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa actttacatg
      661 tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc
      721 cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga cttaaatcac
      781 ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat ctttatttat
      841 gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa
      901 tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga taaacaaatt
      961 gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa acggactctg
     1021 gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac
     1081 ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag cctgcttgac
     1141 gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac gttggacaaa
     1201 gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta taacctacaa
     1261 acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca gatgaccgat
     1321 gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc ccccgaggat
     1381 ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat tttgaaaaac
     1441 gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct ctccggggat
     1501 gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct agctgccata
     1561 tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg cgcgggactt
     1621 ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta cgcggaaagc
     1681 aatgataact atacga
//
LOCUS       DAPBAB010000174         1564 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPBAB010000174 DAPBAB010000000
VERSION     DAPBAB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.190"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..326
                     /locus_tag="RD444_002998"
     CDS             <1..326
                     /locus_tag="RD444_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550288.1"
                     /translation="TTVVDNNQELTPLDRNGIAIGNPTNDLTVTENYLIVSPLLARAA
                     SKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSG
                     TLTRK"
     gene            336..539
                     /locus_tag="RD444_002999"
     CDS             336..539
                     /locus_tag="RD444_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550289.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            750..1334
                     /locus_tag="RD444_003000"
     CDS             750..1334
                     /locus_tag="RD444_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550290.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          553 a          224 c          291 g          496 t
ORIGIN      
        1 caactactgt agtagataat aaccaagaat taactccttt agataggaac ggaatagcca
       61 ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac
      121 ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg
      181 gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt
      241 actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt
      301 attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa
      361 atttttatca agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt
      421 tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag
      481 aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat
      541 gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa
      601 atcacccatt tctggacact ttcttttttt gctttatttt tcaaaaaaac aattcatttc
      661 atgttcataa acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt
      721 acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg
      781 gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt
      841 ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt
      901 ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg
      961 aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc
     1021 aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg
     1081 cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag
     1141 ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg
     1201 aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga
     1261 acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga
     1321 caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag
     1381 tgacaatagt atgagaattt tttgtaagtg tctactgata acttattctc aaatatttag
     1441 ctatttcttc tttacaacat cgaacatatg tttgtataat gtggaagaat ggagtgttat
     1501 ttatgatgga ttattcaaaa gagccagtaa atgattactt tttaatcgat atgaaaagct
     1561 ttta
//
LOCUS       DAPBAB010000175         1559 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPBAB010000175 DAPBAB010000000
VERSION     DAPBAB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.191"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            44..103
                     /gene="ermCL"
                     /locus_tag="RD444_003001"
     CDS             44..103
                     /gene="ermCL"
                     /locus_tag="RD444_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDT7550291.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            151..885
                     /locus_tag="RD444_003002"
     CDS             151..885
                     /locus_tag="RD444_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HDT7550292.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          583 a          246 c          211 g          519 t
ORIGIN      
        1 tgtgctataa ttattctaat ttcataaaaa ggagaattaa tatatgggca tttttagtat
       61 ttttgtaatc aacacagttc attatcaacc aaacaaaaaa taagtggtta taatggattg
      121 ttaatataaa cctcatagga gtgggttata atgaacaaaa aaaatataaa agatagtcaa
      181 aactttatta cttcaaagca tcatataaat gaaattttga gaaatgtaca tttaaataca
      241 aatgataata ttattgagat tggttcaggg aaaggtcatt tcacgtttga attagctaaa
      301 aggtgtaatt atgtaaccgc cattgaaata gatcctaaat tatgtaggat aactaaaaac
      361 aaacttattg aatatgagaa cttccaggtt atcaataaag atattttaca atttaagttt
      421 cctaaaaata agtcatataa gatatatgga aatataccct acaatataag tacagatata
      481 attcgaaaaa ttgtttttga aagcacagct acagaaagtt atttaatagt ggaatatgga
      541 tttgctaaaa ggttgctaaa tacaaatcgt tcactagcac tatttttaat gacagaagtt
      601 gatatatcca tattaagtaa aatccctaga gaatactttc atccaaaacc tagagttaat
      661 agctcgttaa ttgtattaaa aagacaccct tcaaaaatat cactcaaaga tagaaaacaa
      721 tatgaaaatt ttgttatgaa atgggttaac aaagaataca taaaactatt ttccaaaaac
      781 caattttatc aagccttaaa atatgcaaga attgacgatt taaacaatat tagctttgaa
      841 caattcttgt ctcttttcaa tagctataaa ttatttaata gataagttaa ggggtgcata
      901 aactgcatcc cttaacttat cttattttgc ttaaacaatc ttggcccgta aactgcataa
      961 ctcatatgta aatcgctcct ttttaggtgg cacaaatgtg aggcattttc actatttccg
     1021 gcaattactt ccatgtaaag tataacccac tatactttgc aagcaaagtg tgggctcgcc
     1081 gaggggtctt gcagacggct ctttcggctc ttttgagccg agtatcagcg cacccttatg
     1141 gtgcaattag ctgatacctg tttgacgatt tgctgcccta ccttttcggt aggtatgcaa
     1201 acctcaaacc aaaataaaaa cccccttcga cttcgtcagg gggtttttat ttatataaat
     1261 ctccgtaaat aatcgcgatt taataacatc ttcaatttta tttttttgcg tattttagaa
     1321 atgttttcac taaaaaatat attatacaaa aaatacagta atcgattata attctttgcc
     1381 agttaaatat catatcgtta ttaaaatatc ggaaaaaaat atcaacaaca ataaatgcaa
     1441 aagaagacca taaaaattgt ttattactca tacatactct ccatttctat catttttaaa
     1501 tttaccagtc atctctcaaa aatctcctag ctatttttct tttttaacca cataccgtc
//
LOCUS       DAPBAB010000176         1531 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPBAB010000176 DAPBAB010000000
VERSION     DAPBAB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.192"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..1342
                     /locus_tag="RD444_003003"
     rRNA            <1..1342
                     /locus_tag="RD444_003003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="RD444_003004"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="RD444_003004"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DAPBAB010000177         1520 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPBAB010000177 DAPBAB010000000
VERSION     DAPBAB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.193"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>1520
                     /locus_tag="RD444_003005"
     CDS             <1..>1520
                     /locus_tag="RD444_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDT7550293.1"
                     /translation="KSKGYDLDFLKQVMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKN
                     PTLFWLANLMNNPHTVATLDVSTDEKQKVIDQINRALDELNDRSDNERKATDRNKSAE
                     EYLELAEYARKLTSNGEISKQVKVRVHVYGNTIQEVDERAQELIENLKGMDYRAVTYL
                     HKTKRDWQSMFTMFDEQAEWFGNIKGQSLPSLNIGGGFPFHHQTLKDPGGTQYGETMT
                     GGPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFI
                     PFLKEYNGIMVRLDGSDGMINPLEIFATRTYDEASSLYDDGSVKDLKINEAASYQTHL
                     DKVVYQVQLVSPQLKGTMKAEFKTYLNRFYEEYGTVPRGFTSSNSRSNTETQVTGKDP
                     EAYPTFKEFLDFLGQLELPGASQEKKNRKEEMESIVESLCETYGMIFDGHSTIRHLDQ
                     QQLVFFDIDSITGLDRGVQQCQMYMAMTLIWNQALIQGRRQRRLIKEKQMEEQDFK"
BASE COUNT          523 a          264 c          340 g          393 t
ORIGIN      
        1 taaaatccaa agggtatgat ttagattttt taaaacaagt gatgccacaa ggtgggttaa
       61 attttgatga agaatatgtg acgtatggta atggttattc acgatgtgtg acagtttatc
      121 actacacaaa aaatcctacg cttttttggt tggccaatct catgaacaat cctcatacag
      181 tggccacact cgatgttagc acagatgaaa agcaaaaagt gattgatcaa atcaatcgtg
      241 ccttagatga attaaatgat cgatcagata acgaacgaaa agcgactgac cgaaataaaa
      301 gcgcagaaga atacctggaa ctagcggaat atgctcgtaa attgacttct aatggggaaa
      361 tttctaagca agtcaaagtg agagtacatg tttacggaaa cacgattcaa gaagtagatg
      421 aacgagcgca ggaactgatc gaaaatctca aagggatgga ttatcgtgcg gtgacttatc
      481 tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa caagctgaat
      541 ggttcggaaa tatcaaaggg caatcactac catcgttaaa cattggcgga ggtttcccgt
      601 tccaccacca aacgctaaaa gatccaggag ggacacaata tggggaaact atgacaggcg
      661 gcccatttgt ctggaatgcg acgtataaaa atagtgtacg gttgtctacc aataccttgc
      721 ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg gaagcccatt
      781 tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt cctttcttaa
      841 aggaatataa cggtattatg gtgcgtttag acggatctga cggaatgatc aatcctttag
      901 agatatttgc cacacggacg tatgatgaag catccagttt atatgacgat ggatcagtca
      961 aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca cttagacaaa gtggtctatc
     1021 aggtacagtt agtgagtcct cagctaaaag gaacgatgaa agcagaattt aagacctatt
     1081 taaatcgttt ttatgaagag tatggaacag taccacgcgg tttcacttcc tcaaactcgc
     1141 gttcaaatac ggagacgcaa gtcacaggga aagatccaga agcttatccg acgttcaaag
     1201 aatttttaga ctttttagga caactggaat taccaggtgc ttcccaagag aaaaagaatc
     1261 gaaaagaaga aatggaatcc atcgtagaat ccttatgtga gacgtatggc atgatttttg
     1321 atggtcatag tacgattcga caccttgacc aacaacagtt ggtctttttt gacattgact
     1381 ctattacagg tttagatcgt ggggtacaac aatgtcaaat gtacatggct atgacgttga
     1441 tttggaatca ggcattgatt caaggaagaa gacaacgacg tttaattaaa gaaaaacaaa
     1501 tggaagaaca agacttcaaa
//
LOCUS       DAPBAB010000178         1520 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPBAB010000178 DAPBAB010000000
VERSION     DAPBAB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.194"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>1520
                     /locus_tag="RD444_003006"
     CDS             <1..>1520
                     /locus_tag="RD444_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDT7550294.1"
                     /translation="KSKGYDLDFLKQVMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKN
                     PTLFWLANLMNNPHTVATLDVSTDEKQKVIDQINRALDELNDRSDNERKATDRNKSAE
                     EYLELAEYARKLTSNGEISKQVKVRVHVYGNTIQEVDERAQELIENLKGMDYRAVTYL
                     HKTKRDWQSMFTMFDEQAEWFGNIKGQSLPSLNIGGGFPFHHQTLKDPGGTQYGETMT
                     GGPFVWNATYKNSVRLSTNTLLLGMMGAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFI
                     PFLKEYNGIMVRLDGSDGMINPLEIFATRTYDEASSLYDDGSVKDLKINEAASYQTHL
                     DKVVYQVQLVSPQLKGTMKAEFKTYLNRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDP
                     EAYPTFKEFLAFLGQLELPGASQEKKNRKEEMESIVESLCETYGMIFDGHSTIRHLDQ
                     QQLVFFDIDSITGLDRGVQQCQMYMAMTLIWNQALIQGRRQRRLIKEKQMEEQDFK"
BASE COUNT          516 a          272 c          341 g          391 t
ORIGIN      
        1 taaaatccaa agggtatgat ttagattttt taaaacaagt gatgccacaa ggtgggttga
       61 attttgatga agaatatgtg acctatggaa atggttattc acgatgtgtg acagtttatc
      121 actacacaaa aaatcctact cttttttggt tggccaatct catgaacaat cctcatacag
      181 tggccacact cgatgttagc acagatgaaa agcaaaaagt gattgatcaa atcaatcgtg
      241 ccttagatga attaaatgat cgatcagata atgaacgaaa agcgactgac cgaaataaaa
      301 gcgcggaaga atacctggaa ctagcggaat atgctagaaa attgacttct aatggggaaa
      361 tttctaagca agtcaaagtg agagtacatg tttacggaaa cacgattcaa gaagtagatg
      421 aacgagcgca ggaactgatc gaaaatctca aagggatgga ttatcgtgcg gtgacttatc
      481 tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa caagctgaat
      541 ggttcggaaa tatcaaaggg caatcactac catcgttaaa cattggcgga ggtttcccgt
      601 tccaccacca aacgctaaaa gatccaggag ggacacaata tggggaaacc atgacaggcg
      661 gcccatttgt ctggaatgcc acgtataaaa atagtgtacg tttatctacc aataccttgc
      721 ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg gaagcccatt
      781 tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt cctttcttaa
      841 aggaatataa cggtattatg gtgcgtttag acggatctga cgggatgatc aatcctttag
      901 agatctttgc cacacggacg tatgatgaag catccagttt atatgacgat ggttcagtca
      961 aagatttgaa gatcaatgaa gcagcgtcct atcaaacgca cttagacaaa gtagtctatc
     1021 aggtacagtt agtgagtcct cagctaaaag gaacgatgaa agcagaattc aagacctact
     1081 tgaatcgttt ttatgaagag tatggaacag taccacgcgg ttttacttcc tcaaattcgc
     1141 gttcaaatac tgatacgcaa gtcacaggga aagatccaga agcttatccg acgttcaaag
     1201 aatttttagc ctttttagga caactggaac taccaggggc ttcccaagag aaaaagaatc
     1261 ggaaagaaga aatggagtct atcgtggaat ccttatgtga gacttatggc atgatttttg
     1321 atggtcatag tacgattcga caccttgacc aacaacagtt ggtctttttt gacattgact
     1381 ctattacagg cctagatcga ggggtacaac aatgtcagat gtacatggcc atgacgttga
     1441 tttggaatca ggcattgatt caaggacgaa gacaacgacg cttaattaaa gaaaaacaaa
     1501 tggaagaaca agacttcaaa
//
LOCUS       DAPBAB010000179         1500 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPBAB010000179 DAPBAB010000000
VERSION     DAPBAB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.196"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            10..375
                     /locus_tag="RD444_003007"
     CDS             10..375
                     /locus_tag="RD444_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7550295.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            376..>1500
                     /gene="aac(6')-Ie"
                     /locus_tag="RD444_003008"
     CDS             376..>1500
                     /gene="aac(6')-Ie"
                     /locus_tag="RD444_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HDT7550296.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDF"
BASE COUNT          618 a          120 c          253 g          509 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatggt
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      541 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      601 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      661 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      721 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      781 gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt
      841 attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg
      901 gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat
      961 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
     1021 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
     1081 ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta
     1141 aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa
     1201 gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa
     1261 aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca
     1321 gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg
     1381 cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga aagttttatg
     1441 gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt
//
LOCUS       DAPBAB010000180         1475 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPBAB010000180 DAPBAB010000000
VERSION     DAPBAB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.197"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            1..261
                     /locus_tag="RD444_003009"
     CDS             1..261
                     /locus_tag="RD444_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550297.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAVSMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            258..>1475
                     /locus_tag="RD444_003010"
     CDS             258..>1475
                     /locus_tag="RD444_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7550298.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDFGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYTHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDNMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEV"
BASE COUNT          517 a          244 c          274 g          440 t
ORIGIN      
        1 atgaaactga tcaaattatt attattcaat ctttcttgtt taaccgcagt ctttttgtac
       61 tgcggtttat tattttctca actatctatt ttgccaagct accaactata cctaatgagg
      121 attttaaaca taccagctgt ttccatgccg ctagatatta tggaaaagct cattaggaat
      181 ccattattta ctacgatcaa tttgatcgtt attgtcggaa tttcactagt catttataaa
      241 aaagagggga tgaagggatg aattttaaac actcgtggac aagacggtta aaatatgttt
      301 ttcccataat gatgatgtta ggtacattgt ttgggttaaa aacagccaat gtctttgctg
      361 aaaaagttat tgtcgatccg tcaaatccaa tacagaacgt aaagaataat tttattattc
      421 caaaagatgt tcctaatgca aaaacaaata ttactgtaaa cggagcgtca aaagtttaca
      481 aatatcatgt tgatgccaac aaaggtggtt ttacagacga ctacgtagga ttagtggagg
      541 gaaacaaaaa tatacgctat ccttctttcc aaaaggaata cggtgaaaca aaccttgttt
      601 tagaaggcgt aacaaccaac accaaagtca atattgactt tggaaaaatc ggtacgtata
      661 acgggaaaaa agtcaatatc aagctagttt tatctaatat tcacttatac tcggatacgt
      721 tgccgtggaa tctactagat aataactata caaaaactca ttttcgagac gatggctata
      781 aaaatacgaa tggtgcaatg agtaaatcta aaaaaagaac agtactttgg atctcagaca
      841 atcttttctc aggaattgtt taccattcaa cacaaatgaa tgtgcagcta gtagccacat
      901 atgaggacgg tagccccgtt caattttcag gagatacatt tatcagtttt aactctttga
      961 accctgcagg aggaaaaagc acggatctta aaggagaata tacccactat gacaaaatga
     1021 atacaacaga ttggtatgtc agaaaagata cggttctttc agaatttaaa tctttctaca
     1081 acaatctgaa cgtggtaggt ggccacccag gaggaagctc taagctgaca caagcggata
     1141 atgatttcaa taatttgcat gataaattag gagatccgaa gtttggtcaa ggtactgtgt
     1201 cctttaaaat atctgaagca aatccgacgt ttgtgattgg ttcttctaat gttcaaacct
     1261 ggtttacttt atcttctgcg acaatcttta gtgttgtacc tgatcaacct gaaaaaacag
     1321 gggttgataa aaatggaaat aacgtgaacg ataacatgtt gcaagtagga gacacgattc
     1381 aatatcgaat caaacagaag gtgaatcgat taggagtgga tttactagcg gtttacgatc
     1441 gatttgagtt aatcgataat ttaccaaaag aagta
//
LOCUS       DAPBAB010000181         1475 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPBAB010000181 DAPBAB010000000
VERSION     DAPBAB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.198"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            1..261
                     /locus_tag="RD444_003011"
     CDS             1..261
                     /locus_tag="RD444_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550299.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            258..>1475
                     /locus_tag="RD444_003012"
     CDS             258..>1475
                     /locus_tag="RD444_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7550300.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEV"
BASE COUNT          517 a          245 c          275 g          438 t
ORIGIN      
        1 atgaaactga tcaaattatt attattcaat ttttcttgtt taaccgcagt ctttttgtac
       61 tgcggtttat tattttctaa actatctatt ttgccaagct accaactata cctaatgagg
      121 attttaaaca taccagctgc ttccatgccg ctagatatta tggaaaagct cattaggaat
      181 ccattattta ctacgatcaa tttgatcgtt attgtcggaa tttcactagt catttataaa
      241 aaagagggga tgaagggatg aattttaaac actcgtggac aagacggtta aaatatgttt
      301 ttcccataat gatgatgtta ggtacattgt ttgggttaaa aacagccaat gtctttgctg
      361 aaaaagttat tgtcgatccg tcaaatccaa tccagaacgt aaagaataat tttattattc
      421 caaaagatgt tcctaatgca aaaacaaata ttactgtaaa cggagcgtca aaagtttaca
      481 aatatcatgt tgatgccaac aaaggtggtt ttacagacga ctacgtagga ttagtggagg
      541 gaaacaaaaa tatacgctat ccttctttcc aaaaagaata cggtgaaaca aaccttgttt
      601 tagaaggcgt aacaaccaac accaaagtca atattgacta tggaaaaatc ggtacgtata
      661 acgggaaaaa agtcaatatc aagctagttc tatctaatat tcacttatac tcggatacgt
      721 tgccgtggaa tctactagat aacaactata caaaaactca ttttcgagac gatggctata
      781 aaaatacgaa tggtgcaatg agtaaatcta aaaaaagaac agtactttgg atctcagaca
      841 atcttttctc aggaattgtt taccattcaa cacaaatgaa tgtgcagcta gtagccacat
      901 atgaggacgg tagccccgtt caattttcag gagatacatt tatcagtttt aactctttga
      961 accctgcagg aggaaaaagc acggatctta aaggagaata tgcccactat gacaaaatga
     1021 atacgacaga ttggtatgtc agaaaagata cggttctttc agaatttaaa tctttctaca
     1081 acaatctgaa cgtggtaggt ggccacccag gaggaagctc taagctgaca caagcggata
     1141 atgatttcaa taatttgcat gataaattag gagatccgaa gtttggtcaa ggtactgtgt
     1201 cctttaaaat atctgaagca aatccgacgt ttgtgattgg ttcttctaat gttcaaacct
     1261 ggtttacttt atcttctgcg acaatcttta gtgttgttcc tgatcaacct gaaaaaacag
     1321 gggttgataa aaatggaaat aacgtgaacg ataaaatgtt gcaagtagga gacacgattc
     1381 aatatcgaat caaacagaag gtgaatcgat taggagtgga tttactagcg gtttacgatc
     1441 gatttgagtt aatcgataat ttaccaaaag aagta
//
LOCUS       DAPBAB010000182         1465 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPBAB010000182 DAPBAB010000000
VERSION     DAPBAB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.199"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            173..691
                     /locus_tag="RD444_003013"
     CDS             173..691
                     /locus_tag="RD444_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDT7550301.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            698..1210
                     /locus_tag="RD444_003014"
     CDS             698..1210
                     /locus_tag="RD444_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550302.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          575 a          170 c          218 g          502 t
ORIGIN      
        1 aaaaaaaata gagccgtctc aacttttggc gaggtagacg agctctaaag ctttatttac
       61 atgccatccg ctttgaacgg actacactgg gcatgtgaca gcatgccctt tcatttatat
      121 tatatctcat tcttttatat aatgaaagta aataaatcat ggaggataaa atatggataa
      181 atttaataga ttaacattaa taaatcagtt tgaaatttta aaagctttaa atcctaacga
      241 agaaaaatac tatgctgaaa aaatagaaat actaacagaa ggttacgaat atcactatag
      301 tgaaatattt gaaaatattt ctgatccatt gccagaagaa gattcgcggt ttgttctaga
      361 catattaaat atgtatagag atattacttt tagtaaaaat aaattaaaag atataaattc
      421 aattgataat tattatatac aatttaaagg ttttgacttt aatagctcta ccgaatttaa
      481 gttagcactt tatgcacaat tttttattga aaaattaaat aggtttcaag aaatagttga
      541 agatagtgat tttaacactt ttaatagcca taaaccgatg agaggtactt atataaaatt
      601 tctcgaaaat tataatgatt taaaatctac caataattat caatttggaa acttgtctta
      661 tgagatgata agtaaagttc taagtttgtg atgataaatg aaattaaata gtataaatga
      721 tttatttgct attataggtg ttattgctaa catacttttt tcgtgtttga attttagatt
      781 tactcaaaag aacaaaaaga acaaactcag tatagaacaa atgaaaaaag acattgaaac
      841 tgatttaaaa aatgaccata aagaaagaag aaaaattgaa gaatatactt ctttaatgga
      901 aaagtgtaaa gcaactgatg caatttttag ctttttaaga aaaagtgatg agataatcac
      961 acgtgaaaat atagatttag aaaatgagat tagtaaactt gaatctatct tttccgatat
     1021 aaattctctt aagtatttaa tcgtcgaaga aaattcggat agctttttag aatttgaaaa
     1081 atatctaaca gatcttatag ataatagaaa taatgaatta atgagacaaa acattgcaag
     1141 attccttact aaattagttt ctgaatggaa aagtattatt tctcaaaata tggataaatt
     1201 aagatattaa ggatgttagt tttaaaataa actaatgtcc ttatttttgt gttcacattt
     1261 ctgttaccta agaaatactt aaaggaggtg atgtagccag caacgactac aacttgaacc
     1321 cgctatctac agtttatttg ttgtttcttt tgggtatact ctaatggtac aaatccatat
     1381 ttgcttaaag cgttgatttt tcagcggttt ttctttattg caaagtgtac cctaagacct
     1441 gtatactaat agtaaataga ttgga
//
LOCUS       DAPBAB010000183         1460 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPBAB010000183 DAPBAB010000000
VERSION     DAPBAB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.200"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(10..1149)
                     /locus_tag="RD444_003015"
     CDS             complement(10..1149)
                     /locus_tag="RD444_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDT7550303.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          382 a          314 c          264 g          500 t
ORIGIN      
        1 aaaaaaatct tacgctaagt aggataaaat aattccttga tatttttcga tcatatccaa
       61 ataattatcg atttcaacat actcatctac ttgatgaggc aaagtaacga caccaggccc
      121 aaagacgaca aagtcgaagg aatggttagc tttcgtaaat tcggctgcat ccgttgttgc
      181 tgccgctcca actaaaggta atggctggct gaattgttgc tgaatgcaat gaatcaaagg
      241 agaatctgga tcagctttga caggtatttt gttgtaatcg atcatcaatt ccaaatgata
      301 gtctgtctct tgattcaatt cattgacgat tgattgcagc aatgcaatga ttttatcatt
      361 tgggtattct ggaatcgatc gaatgttccc ttggagctgc gcatggcttg ggatgctgtt
      421 gacttgattc ccgccactga tcaatgtaac attatggatt gtttttccta ggacaggatt
      481 ctcgattgtt tcagcgaggt gattcatttc agcatttgct ttcgtaatga attcatttag
      541 gtgattgatt gcattataac cttggtctgg catagagcta tgcgcttctt ttccgtgcga
      601 ggtgactgta tagttgattg atcccatgtg cgtatacatc aaactgtaat tggttggctc
      661 tccgatgatc aaggcatcta agtcatctac atagcctgct ttcgtgagct gttccccacc
      721 tagttcaccc acttcttctc cgacagtggc taataatttg actgttccgt taaatggttt
      781 tcctgactct ttcagttcga tcatggcaat caccattgct gccaagccac tcttcatatc
      841 tgtagcacca cgtccgtaca aacggttccc atggatttct gctgcaaagg gtgcataggt
      901 ccaagacgat tcatcaccag ccgccacgac atccatatgc ccagataggc ctaacacttt
      961 tcctgattgg cccttttggt aacgagcaat caaattatct ctaccatctc tgtaagaaac
     1021 aatctctcct gttatgtcat gttttgccaa aagcttattc aaataggctg ctacttcacc
     1081 ttcgtttcca ttcactgatt taattcggat gatctcttgt aaaatcgcga ttttttcttc
     1141 ttttttcatc taatcctctc ccttgccatt tctttttatt gtatttgagt gtgctcgaat
     1201 tttcaaatag gagcaaccaa agtaaaaatg aagaaagaga gccactttat gaaaaatatg
     1261 ctgctacttc cctcctattt tatttatacg agaggacttg ctcatagatt cctattctac
     1321 tgttctatcc ttaaaagctc ttatatgatg ctagttattg ttagctttcc tttttcaagg
     1381 atgatctttt cttgtaccat tctttgtaaa catcatatac agaagctagt tgcaaaaaaa
     1441 ctctctacct gctgatattt
//
LOCUS       DAPBAB010000184         1459 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPBAB010000184 DAPBAB010000000
VERSION     DAPBAB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.201"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(26..110)
                     /locus_tag="RD444_003016"
     tRNA            complement(26..110)
                     /locus_tag="RD444_003016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Leu,seq:caa)
     gene            complement(128..199)
                     /locus_tag="RD444_003017"
     tRNA            complement(128..199)
                     /locus_tag="RD444_003017"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Gln,seq:ttg)
     gene            complement(209..281)
                     /locus_tag="RD444_003018"
     tRNA            complement(209..281)
                     /locus_tag="RD444_003018"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:His,seq:gtg)
     gene            complement(343..427)
                     /locus_tag="RD444_003019"
     tRNA            complement(343..427)
                     /locus_tag="RD444_003019"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(391..393),aa:Leu,seq:caa)
     gene            complement(448..518)
                     /locus_tag="RD444_003020"
     tRNA            complement(448..518)
                     /locus_tag="RD444_003020"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(484..486),aa:Cys,seq:gca)
     gene            complement(564..635)
                     /locus_tag="RD444_003021"
     tRNA            complement(564..635)
                     /locus_tag="RD444_003021"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Gln,seq:ttg)
     gene            complement(647..719)
                     /locus_tag="RD444_003022"
     tRNA            complement(647..719)
                     /locus_tag="RD444_003022"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(684..686),aa:His,seq:gtg)
     gene            complement(722..795)
                     /locus_tag="RD444_003023"
     tRNA            complement(722..795)
                     /locus_tag="RD444_003023"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(761..763),aa:Trp,seq:cca)
     gene            complement(802..882)
                     /locus_tag="RD444_003024"
     tRNA            complement(802..882)
                     /locus_tag="RD444_003024"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(846..848),aa:Tyr,seq:gta)
     gene            complement(884..959)
                     /locus_tag="RD444_003025"
     tRNA            complement(884..959)
                     /locus_tag="RD444_003025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Phe,seq:gaa)
     gene            complement(995..1067)
                     /locus_tag="RD444_003026"
     tRNA            complement(995..1067)
                     /locus_tag="RD444_003026"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Asp,seq:gtc)
     gene            complement(1077..1149)
                     /locus_tag="RD444_003027"
     tRNA            complement(1077..1149)
                     /locus_tag="RD444_003027"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1114..1116),aa:Val,seq:tac)
     gene            complement(1165..1236)
                     /locus_tag="RD444_003028"
     tRNA            complement(1165..1236)
                     /locus_tag="RD444_003028"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1201..1203),aa:Glu,seq:ttc)
     gene            complement(1251..1339)
                     /locus_tag="RD444_003029"
     tRNA            complement(1251..1339)
                     /locus_tag="RD444_003029"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Ser,seq:gga)
     gene            complement(1379..1452)
                     /locus_tag="RD444_003030"
     tRNA            complement(1379..1452)
                     /locus_tag="RD444_003030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1416..1418),aa:Asn,seq:gtt)
BASE COUNT          415 a          385 c          334 g          325 t
ORIGIN      
        1 aagaaagttg ctatattgat aattataccg gcggccgggg tcgaaccggc acgtcctcaa
       61 cggacactgg attttgagtc cagcgcgtct gccaattccg ccacgccggc aaatttaaaa
      121 ataataactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
      181 accgcttggc tataccccaa gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc
      241 ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatcataaa agatgttatt
      301 taatttctca ataaaaaact aagatgaaga catcttagtc aataccggcg gccggggtcg
      361 aaccggcacg ccctcaacgg gcactggatt ttgagtccag cgcgtctgcc aattccgcca
      421 cgccggcaag ttgatttatt agtactaagg cggtaaccgg atttgaaccg gtgatgaagg
      481 ttttgcagac ctctgcctta ccacttggct ataccgccat tatactatta tcaatctaca
      541 aaataaagaa taaattatag aaactggggt agctggattc gaaccaacgc atgagggagt
      601 caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg aatgatggga
      661 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
      721 atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc tgttctacct
      781 ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac cctaaggaac
      841 ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc gcgggacaga
      901 atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc taccgcgcca
      961 tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat cgaacccgcg
     1021 atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt tcattatgga
     1081 ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc ccagctgagc
     1141 taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt
     1201 gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta cggagagtaa
     1261 gggattcgaa cccttgagac agtttgcacc atctacatga tttccaatca tgctccttcg
     1321 gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa aatgagaact
     1381 ccggcagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg
     1441 agctatgccg gaataacat
//
LOCUS       DAPBAB010000185         1456 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPBAB010000185 DAPBAB010000000
VERSION     DAPBAB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.202"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            108..1040
                     /locus_tag="RD444_003031"
     CDS             108..1040
                     /locus_tag="RD444_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDT7550304.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="RD444_003032"
     CDS             1112..>1456
                     /locus_tag="RD444_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550305.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAPBAB010000186         1425 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPBAB010000186 DAPBAB010000000
VERSION     DAPBAB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.203"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..618
                     /locus_tag="RD444_003033"
     CDS             <1..618
                     /locus_tag="RD444_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDT7550306.1"
                     /translation="PFYKEEESLMPYFALAAQRAGAVGIRANSVRDIIEIRKCVSLPM
                     IGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDGLSINSFITKIKEK
                     YPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGIC
                     VIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            632..>1425
                     /locus_tag="RD444_003034"
     CDS             632..>1425
                     /locus_tag="RD444_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7550307.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAG"
BASE COUNT          451 a          202 c          300 g          472 t
ORIGIN      
        1 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
       61 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
      121 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
      181 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
      241 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
      301 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
      361 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
      421 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
      481 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
      541 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
      601 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
      661 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
      721 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
      781 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
      841 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
      901 tggtggagta gcttatctag tatatactgc aactattagt gggtttcttg aacttttttc
      961 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     1021 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     1081 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     1141 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     1201 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     1261 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     1321 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     1381 ccacactggt ctgtttactt atggtactag attttttgca ggaag
//
LOCUS       DAPBAB010000187         1371 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPBAB010000187 DAPBAB010000000
VERSION     DAPBAB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.204"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            88..>1371
                     /locus_tag="RD444_003035"
     CDS             88..>1371
                     /locus_tag="RD444_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7550308.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTF"
BASE COUNT          432 a          270 c          260 g          409 t
ORIGIN      
        1 acatcttttt tgtataaaaa aatatagaaa agacaccctg aaatccttta gaattaattt
       61 ggctaccaat tcataggagg attcaagatg tctcatactc agcttatcaa agatacccta
      121 aatattctag acctaaatat ctatttcgag gaaaattgtt taactaaaga aaaatacaaa
      181 gggcaaattt gcttgattta caaaggaact cttctgtaca agccagaaga gtgttcccat
      241 tgtctttgtg tcgtcccctc taggatcatt cgttggggga cgacaaccgt tcggttgtta
      301 ctgaatgatg tgtctgagta tcgcacttat ctggagctga agaaacaacg atttaaatgt
      361 aagtcttgcc aaagaacatt cgtagcggat acctctgttg ctgaaaaaca ctgtttcatt
      421 agtcaaaaag ttcgttggtc tgtcattgct cgcttaaaag agaatacttc catgacggaa
      481 atcgctcgtc agaaaaatat ttccacttct tcggtctatc gtgtcatgaa gcgattctac
      541 cgcccattga atcctttcaa acagactttg ccaaaggtct tatgctttga tgaattcaaa
      601 tcggttcgaa gtgttgccag tgcaatgagt tttatcatga tggacggaca aagccatgca
      661 ttattggata ttgtcgaaaa tcgacgcttg ccttatttag aacgctattt ttctcggttt
      721 tcattagcga ctcgtgaagc cgttcaattg attgttattg atatgtatgc gccttatgtt
      781 tcgttggtga aaaagctttt cccgaatgct cagttgatca tcgatcgttt ccacatcgtc
      841 caacatattg gcagaacttt cttgaatcat cgagtgaaag aaaccacggt acgacttaaa
      901 ggaaattccc actctcaacg agggttaggg aaaaaattga agcgctattg gaaactccta
      961 caaaaagaag aagcaaagtt aaactatgaa aaacgggttt ggcgtgccag ttttaaagat
     1021 tatttgactg aaagtgagat tgccgatcga ctgttagctg cttgtcccaa cttacgccaa
     1081 ggctatcagt tataccaaga cattctttat gcggtaaaga aaagagatca gtcactattt
     1141 gaggattgtt taactagaga cattagcggt ctgccagaaa cctatacaac cactttacga
     1201 acgtttaaaa agtttctgcc acagatccaa aatgcgcttc actacagcta ttcaaacgga
     1261 ccattggaat gcttaaacaa ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc
     1321 aacttttaca atttcaaact acgaattatg attcgtcatg gcaaaacatt t
//
LOCUS       DAPBAB010000188         1362 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPBAB010000188 DAPBAB010000000
VERSION     DAPBAB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.205"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            82..>1362
                     /locus_tag="RD444_003036"
     CDS             82..>1362
                     /locus_tag="RD444_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDT7550309.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAPBAB010000189         1350 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPBAB010000189 DAPBAB010000000
VERSION     DAPBAB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.206"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            70..>1350
                     /locus_tag="RD444_003037"
     CDS             70..>1350
                     /locus_tag="RD444_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDT7550310.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          459 a          255 c          257 g          379 t
ORIGIN      
        1 aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac tgagaggacg
       61 atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa agatttgatg
      121 attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt cgatgctgtt
      181 ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga tggaaacggg
      241 aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg tccgctttga acaatacaat
      301 cacatgcctt tggttatgcg cttaaaaaag cagcgctata cctgtaaaaa ctgtcgaacc
      361 cattggacgg ctcaaagtta ttttgttcaa cccagacatt caatcgcgaa tcatgtcaga
      421 tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc gaaaaattgt
      481 caggtatctt tgaccaccgt tattcgtacg ttgaaagagt ttaaaagcta cttaccaaag
      541 caatctaaga ggattctccc cagagtattg atggttgatg aatttcgttc gcacgcttcc
      601 atagaagata aaatgagctt tatttgcgca gatggcgaaa caggacaatt aattgatgtc
      661 ttgcctacgc gtaaattgcc tcgattaaca agctatttct taggctgcgc taatcctgaa
      721 gaagtaaaat ttttagtcac agacatgaac gccgcctact tccagcttac caaacgtatt
      781 ctgccaaatg cgaaagtagt gattgatcgg tttcatatta tcaaacacat gaatcaagcc
      841 tttaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagtaggaca gaaaagtcag
      901 gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat
      961 tatgaataca aaacatggaa aagttttcga gcaccaaaat acccatttct tacggaagcg
     1021 atgatgattg atcgattgct tggcttttct acgtccctga aggaggcgta tccctatttt
     1081 catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc cttattggca
     1141 gaacttcccg aaacgttaga tgacggcttt cgggaaaagc tccaaaacct tctgacctat
     1201 gaagaaggca tcaccaacgc gatgatctat ccttacacca atggaaaaat agaagcgaag
     1261 aatacccata taaagacaat gaaacgagta tcctacggat ttaaatcatt tgagaacatg
     1321 agaattagaa tctttttgat caatcaatta
//
LOCUS       DAPBAB010000190         1272 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPBAB010000190 DAPBAB010000000
VERSION     DAPBAB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.208"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(118..594)
                     /locus_tag="RD444_003038"
     CDS             complement(118..594)
                     /locus_tag="RD444_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7550311.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(607..>1272)
                     /locus_tag="RD444_003039"
     CDS             complement(607..>1272)
                     /locus_tag="RD444_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7550312.1"
                     /translation="MYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWL
                     YVIHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWA
                     FIYFFVFTFFIKKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIES
                     VEACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILG
                     KDN"
BASE COUNT          453 a          236 c          163 g          420 t
ORIGIN      
        1 gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt ctgttcacca
       61 acttcatttg acaaatatcc tatttagctt attttatagc tttttatata cactttttta
      121 aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta ttattaaatc
      181 attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac cagattttat
      241 tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat cattttcttg
      301 aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa tatgaatcaa
      361 ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa atatatttgt
      421 tactcgacca gccaatggag cataaatttt tgaaccatca ggtatgacgg caaaaccatc
      481 acctagtaat ttttgtgaaa agacaggatc atttacttct gtgatggaaa tgatttttcc
      541 ggaacaaggt gcataaaaag tttcttgttt tttctttttg aaaatttcaa acatattaaa
      601 aacctcctaa ttatcttttc ctaatatttg attgatttct tgacttaata agtcagcttt
      661 ccctccaaaa acagcttgga tgccaccctt cacttcgaag acagcagttg caccagtgtc
      721 ttttattgtt tgtttttgaa taatctcccc attcgtaaca gcaacacgca aacgagttgc
      781 acaagcttca acagactcaa tattttcttt tccaccaaga gcttcaataa tgataagaga
      841 ctcttcatgt aatgatgtat ttttgtttat tactttttca gaatgaactt ctgattcttc
      901 ttccattcca ggaatagcta ccttaaattt tttgataaag aatgtaaaaa caaagaaata
      961 tataaatgcc catgcaatac caaaaggaat taattttaac caattagttt tgtcattccc
     1021 ttgaagtaca ccgaataata aaaagtcgat caaaccacct gaaaaggaat tccctacacg
     1081 aatatttaag atatcagcaa aataaaaaga taatccatct aagaatgcat gaatcacata
     1141 aagccaagga gcaacaaata aaaacatata ttcaactggt tcagtaattc cagttaaaaa
     1201 tgatgtaagg gccccgctaa aataaagccc accattcttt ttacgatttt tcttaggaat
     1261 tgcacgatac at
//
LOCUS       DAPBAB010000191         1262 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPBAB010000191 DAPBAB010000000
VERSION     DAPBAB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.209"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..1218)
                     /locus_tag="RD444_003040"
     CDS             complement(<1..1218)
                     /locus_tag="RD444_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7550313.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKIL"
BASE COUNT          460 a          197 c          146 g          459 t
ORIGIN      
        1 taaaatctta atcggtttaa catttgttaa aaataaatca taataatcta acacataata
       61 ataatccaaa aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt
      121 tgcttgtaat tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt
      181 catttcttcg atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact
      241 tctttcatga aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa
      301 acggaacatg ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt
      361 ttgtcgctga tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc
      421 aaaagaataa attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat
      481 ttgttttgac cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata
      541 ttcattatct actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa
      601 agaaaataca cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac
      661 tatatacttc tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg
      721 tgtagaattc caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa
      781 ttctaactga aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt
      841 cgcatatctg aaaacagtag atacactagt aaattgctct ttagcaaaat cttttagaac
      901 acttctagga tatttataca taaaatcaat caaatgataa ttataagaag ctttataaaa
      961 atgaagataa atattataaa taggaaaatc tttctctttt tttatgaaaa atcgtttatt
     1021 atcttcattt ttcagaagga tattaccatt atattgctta aataaatcct ctagttcttc
     1081 tatatagtgt aagactgact tctccgtaac attaagtaaa tttctaagtt cttccattgc
     1141 acaccaattg tctttttcta acaaataatc taataaaact acttttttga aagatgtttt
     1201 agttaaaaaa gtttgcatca tttcacctca ataagttact tttgtttatt ttaatcaaag
     1261 aa
//
LOCUS       DAPBAB010000192         1246 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPBAB010000192 DAPBAB010000000
VERSION     DAPBAB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.210"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            200..742
                     /locus_tag="RD444_003041"
     CDS             200..742
                     /locus_tag="RD444_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550314.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="RD444_003042"
     CDS             898..>1246
                     /locus_tag="RD444_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDT7550315.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          352 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaaaacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAPBAB010000193         1216 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPBAB010000193 DAPBAB010000000
VERSION     DAPBAB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.211"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>1216
                     /locus_tag="RD444_003043"
     rRNA            <1..>1216
                     /locus_tag="RD444_003043"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          366 a          241 c          359 g          250 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggtaggagag
     1081 cgttctaagg gcgttgaagg cagatcgtga ggactgctgg agcgcttaga agtgagaatg
     1141 ccggtatgag tagcgaaaga caggtgagaa tcctgtccac cgaatgacta aggtttcctg
     1201 gggaaggctc gtccgc
//
LOCUS       DAPBAB010000194         1154 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPBAB010000194 DAPBAB010000000
VERSION     DAPBAB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.212"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            122..1154
                     /locus_tag="RD444_003044"
                     /pseudo
     CDS             122..1154
                     /locus_tag="RD444_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          388 a          224 c          225 g          317 t
ORIGIN      
        1 tttatataat aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa
       61 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      121 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      181 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      241 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acggggaatt ctcgtaacgg
      301 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      361 ccgcaacggc gagttcaagc aacagactgt tcctgcttat agacggacga atgatatctt
      421 agaggagacc gtcattcacc tcttccgaaa aggtattacc atgtcggaaa tcgcagactt
      481 gattgagaaa atgtatgggc atcactacac gccccaaacc atgtccaaca tgactaaagt
      541 gctgactgaa gaagtaaatg catttaaagt tagaactcta aatgataagt atgtcgctat
      601 tttcatggat gctacttata ttcctttaaa acggcaaacc gtctccaaag aagcaatcta
      661 tattgccatt ggtatacgag aagacggcac taaagaagta ctaagttatg cgattgctcc
      721 gactgaatcg acctacgttt ggaatgagtt gttacaggac atttactcca gaggggttca
      781 tgatgtctta ctgtttatta ccgatggctt aaaaggcatg aaagatacga ttcaccagat
      841 ttatcctaaa gctaaatacc aacattgctg tgttcacgtt tcccgtaata ttgctcataa
      901 agtacgtgtc aaagatcgaa aagaaatctg tgatgatttt aaggctgttt atcaagctag
      961 ctcaaaggaa gaggcaaata cctttttagg gagtatgatt gagaagtagc aaaaaactta
     1021 tcctaaagtg acgcagtcac tgataaaaaa tcaagattta ttgacttttt atgagtttcc
     1081 gcctggtatc cgccgaagca tttactcaac taatttaatc gaatctttca agaaacaaat
     1141 caagaaatac agcc
//
LOCUS       DAPBAB010000195         1125 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPBAB010000195 DAPBAB010000000
VERSION     DAPBAB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.213"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(235..828)
                     /locus_tag="RD444_003045"
     CDS             complement(235..828)
                     /locus_tag="RD444_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550316.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(829..>1125)
                     /locus_tag="RD444_003046"
     CDS             complement(829..>1125)
                     /locus_tag="RD444_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550317.1"
                     /translation="FSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLVHLPLFFALLNYG
                     LWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNYHGTYKEDE"
BASE COUNT          314 a          231 c          178 g          402 t
ORIGIN      
        1 tagttgcttt aatggatcaa gtactttttg atcattttgt ggtgaaaaag ctgttttaat
       61 tgcttctaac atgtacagtc acctcttctt gtttgattct ttacttggtt aaaagattgc
      121 ccttttccca acttacccag gcttaaagtg tgaggaaatt ttctagtggt ctcttccttc
      181 ggaacatctt tttctaacca tatcaacgga tatttcattc tctcttctcc attcctactc
      241 atcattcata ttgttcattc gatacagaat cttgatcaat tcttttttgt agagtggttt
      301 taaccctaaa atgccactag atcgcattaa ctgacggttt ctggtttgta aatccttgat
      361 tgtctttcct tcaatgacga aaaagaagct taagtcagga cgttcttgtt ctgctcgttt
      421 caattcctgg atttcttcga ctaatttctt ttcatatttt tttaatcgtt gtagatttct
      481 tcctaaggac tgttccatac gatttttatc taataattgc cgcttttgca cgatttgttc
      541 cgttagatcc gtctctgtgg tcaaactaac taacttaatt gttggcacat acgtacgcat
      601 gacggtggtc aactgattca ttcgttctag tttctcttgt tcatttaatc cttccacgtc
      661 tgtagaaact acacgataat aacgttgaaa atgatcttct ctagtaatta agactccctc
      721 ttctgtacag gatttatacg gaaataagtc caagatagag cgctcttctg gttttccaaa
      781 ctgcaaaatg gatttccttt caactgaatt ttgtcctttg actcgcattt attcatcctc
      841 cttatacgta ccatgataat ttcggttagg atctaacgca tgataatggt tcttgtccgc
      901 aaaaaaaata gactcgataa tcgcaaaaat ttgtcgttga cctgggttat tatagggtcg
      961 ccaaacaatc actaacgcta tcgcaatcca taagccataa ttcaagagtg caaaaaagag
     1021 tggtaagtgg acaagattat tgaccatttt cgcccaaaaa aagcagccac caagcgttaa
     1081 tagatctaaa aagaaaaaag cgaaaaactt ccattctcca ctaaa
//
LOCUS       DAPBAB010000196         1122 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPBAB010000196 DAPBAB010000000
VERSION     DAPBAB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.214"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..1004
                     /locus_tag="RD444_003047"
     CDS             <1..1004
                     /locus_tag="RD444_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDT7550318.1"
                     /translation="KKLNTLVTIENPFRVKQFSRMFDENKTDRNDALRIADFLRIQRF
                     TTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFS
                     ATLISLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGA
                     LAQESINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGL
                     IAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSV
                     KNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          400 a          219 c          195 g          308 t
ORIGIN      
        1 taaagaagct caacacactt gtaacgattg aaaatccttt tcgggtgaaa caatttagtc
       61 gcatgtttga tgagaataaa actgatcgaa acgatgcatt aagaatcgct gatttcttgc
      121 gtattcaacg attcacaact tcccctatca aagaagagaa atacatggcc ttacagcgtt
      181 taacacgtac tcgctatcaa cttattaagc aattgattcg tacaaagcaa cattttcttg
      241 aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa tcgacgagtc
      301 tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat gaacttgctg
      361 agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc cgtttcaagc
      421 aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac cgcctaggtg
      481 ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa atacgagcac
      541 ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt gtcattccag
      601 aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc ggtttaatcg
      661 ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa tacgctggat
      721 taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct ctgacaaaac
      781 aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta aaaaattatc
      841 ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag catcaacaca
      901 aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg ctactacgta
      961 aacaccaact ctatacgcca ccaaggagtg tgatagagaa ataacaatcc gttattgacg
     1021 caaatccttg aatcaacccg aaaaaaattt gattttgatc gggtctagtt tcgtgcgctt
     1081 caaaacatat tactcaataa agaaaatctt aaatttcatc tt
//
LOCUS       DAPBAB010000197         1091 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPBAB010000197 DAPBAB010000000
VERSION     DAPBAB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.215"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="RD444_003048"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="RD444_003048"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDT7550319.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DAPBAB010000198         1090 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPBAB010000198 DAPBAB010000000
VERSION     DAPBAB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.216"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..280
                     /locus_tag="RD444_003049"
     CDS             <1..280
                     /locus_tag="RD444_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDT7550320.1"
                     /translation="KRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLS
                     QGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            623..879
                     /locus_tag="RD444_003050"
                     /pseudo
     CDS             623..879
                     /locus_tag="RD444_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          379 a          164 c          198 g          349 t
ORIGIN      
        1 taaacgtaat cccttcgtat ctgaatatgt taaacgactt gcaaaaggta tttgtcaact
       61 ttgccaagaa aaagggccat ttataaaaga cggtgtacct tatttacatt gtcatcatat
      121 agaatatttg tcgcaaggtg gaaaagatgt gattgaaaac tgtatcgcgt tatgtcccaa
      181 ttgtcatgct agaattcatg aacttgagtt acctgacgat aaagaaaaat tgctgaagat
      241 cgttgaaaaa agagaaaata gcttttctaa aataccgtaa tttattttta taggacaaat
      301 aagctaatgt gagctatgcc ttctatataa gactaagttc tattataagt aacgaagtaa
      361 ttctcgttcc ttcctgtagt aatatttttt aaataaccta taggcttctt caaaagtaca
      421 tcctccagta aattctaagt actggaggat tctttttcta gtgttgcttt actcatgaaa
      481 aagtagttgg ctacttcatc aaaatttctg aactttttta atgtataata aagagctcga
      541 tcatttacta caaggatgct agctcctaca ttttcacagt tttttagcaa gtaaattttt
      601 ctaaaatcac atcaggaaat gcatgagatt ctttaagaaa ggcaattata gggctatgat
      661 tacggttcgt gtaagtgacg ccaaaaaaga atggctttct tatatggctg aattttatgg
      721 gattacttta tctgaacttc taaaaaacta ttcgatggaa caactagaag atgaatacaa
      781 tcgtcaaacg gctgaaattg cccataaaca ttggataaga agtgggaaaa caacagtatt
      841 catggaagaa tccttaatga atttggtggg ctggaatgac ctgccgttta aaattcacgc
      901 ccgaaattca gaagcagctc tgattggcaa atacgtgtat tggtagagat agaagataat
      961 caattcgttg ttaccgctat tcaagtaggg caccgaagaa atgtctatga ttaagagact
     1021 gtttattttt acaaaaaaat aataactgga atctagttaa aactcttgaa gtgagagatt
     1081 tgactagatt
//
LOCUS       DAPBAB010000199         1062 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPBAB010000199 DAPBAB010000000
VERSION     DAPBAB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.217"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(37..993)
                     /locus_tag="RD444_003051"
     CDS             complement(37..993)
                     /locus_tag="RD444_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550321.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAPBAB010000200         1057 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPBAB010000200 DAPBAB010000000
VERSION     DAPBAB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.218"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            1..369
                     /locus_tag="RD444_003052"
                     /pseudo
     CDS             1..369
                     /locus_tag="RD444_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            complement(454..711)
                     /locus_tag="RD444_003053"
     CDS             complement(454..711)
                     /locus_tag="RD444_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDT7550322.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(704..973)
                     /locus_tag="RD444_003054"
     CDS             complement(704..973)
                     /locus_tag="RD444_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7550323.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          337 a          204 c          156 g          360 t
ORIGIN      
        1 ttagatccat taacaacaga acttgttgtt atgagtcgtg ctgacaacac tggatcaggg
       61 ttgattttag cttcttcacc agctgcaaag aagaagtacg gtatttctaa tgtcagcaga
      121 ccacgggatc taccacagcc ttttcctaaa acgttacatg tcgttccacc acgcatgaat
      181 ctctatatta agcgaaacat gcaggtaaac aatattttca gaaggtatgt agcagacgaa
      241 gatttattga tctattcgat cgatgaatcg atcttaaaag tgaccagatc actgaatctt
      301 tttacgactg aaggaacaag aagtcagcgc aggcagaaac ttgctcaaat gatccaggaa
      361 catattaaat gaattgctaa gaaacgtaat gtaatgaaaa aaatagaggt caaaaactta
      421 aaagtttctg acccttccct tacttccaat tggttaatag tgatcttttg cagaataaat
      481 aaatatcgtt tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga
      541 ccactttcca gataaatcat gctttaatgg ctcaggtttt cctaatccag caaagggaga
      601 acgatcgata tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc
      661 atgccaataa agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa
      721 cctcgattaa gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa
      781 ttttttccat gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat
      841 ctctttttga taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt
      901 cagcatcctc attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag
      961 ctactgcttc cattcctttc accccttatt tatttctata aaacaattgt acattatatt
     1021 gtacaattaa gcaaatgatt aatattctaa cttctta
//
LOCUS       DAPBAB010000201         1044 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPBAB010000201 DAPBAB010000000
VERSION     DAPBAB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.219"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            381..>1044
                     /locus_tag="RD444_003055"
     CDS             381..>1044
                     /locus_tag="RD444_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550324.1"
                     /translation="MKRFLKIFIFLEIILFVYIFNTSIYNIYEKNNIATDNLKGYVLE
                     ETSPEILDKFYTIFTEEYSQNKLELINNKLTSTDKSVYDLYCYPLNEFVQKQPISNSI
                     LLQYHKLQKEDFLDSVGVFYTDLQANEIREIESQLAVTISVFENEAIPYSMILELYLL
                     NFVILFVVLQIIYCIYTSDSLKKKGIKKSMGFSAMHILKEQINNIVKYFIVICSVLFI
                     LLN"
BASE COUNT          358 a          135 c          176 g          375 t
ORIGIN      
        1 cgatttcttg aagtgagtaa ggaatgcttt gaatggccct ttttacaagc tgcgcatctt
       61 ttttatttcc gcaagaatgg ccaataattt cacgattgaa taaatcaatc atcaaacaaa
      121 tataagccca atttgtcccg acccgaacat aagttaaatc cgtgacaacc gctgacaatg
      181 cttttgtttg agtaaacttt cgatctaagt gattcgcaat tttcttttga ttgctttggc
      241 acgatcataa agacgggagc agatattgtc tattgatata atgatagtag acaaacagag
      301 ttggctgatt agctaaagca tatcgagtat aatttgaata ttagacgcat gatttatcag
      361 tgtaagaaag ggtgaggatt atgaaaaggt ttttgaaaat atttatcttt ctcgaaataa
      421 tactttttgt atatattttt aatacttcta tttacaatat ttatgaaaag aataatattg
      481 cgactgataa tttaaaagga tatgttttag aggaaacatc gccggaaatt cttgataaat
      541 tttatacgat atttacagaa gaatattccc aaaataaatt agagcttatc aataataaac
      601 ttacttctac ggataaatct gtctatgatt tatattgtta tccgctaaat gaatttgtgc
      661 aaaaacagcc tatatcaaat tctattctgt tgcagtatca taaattgcaa aaggaagatt
      721 ttttggatag tgtcggggta ttctatacgg atttgcaagc caatgaaata cgtgaaatag
      781 aatctcaact ggctgtaaca attagtgttt ttgaaaatga agctatcccg tatagtatga
      841 tattagagtt gtatcttctt aattttgtaa ttttgtttgt tgtacttcaa ataatatatt
      901 gtatttatac atctgatagt ttgaaaaaaa aaggtattaa aaaaagtatg ggcttttctg
      961 ctatgcatat tttaaaggag caaatcaaca atatagtaaa atattttatt gtgatatgtt
     1021 cggtgttatt tatcctgctg aatt
//
LOCUS       DAPBAB010000202         1014 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPBAB010000202 DAPBAB010000000
VERSION     DAPBAB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.220"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..979
                     /locus_tag="RD444_003056"
     CDS             <1..979
                     /locus_tag="RD444_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7550325.1"
                     /translation="KLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAII
                     SLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTID
                     DLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINEC
                     IEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYI
                     LDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDK
                     IFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          449 a           77 c          134 g          354 t
ORIGIN      
        1 aaagcttaat gatggtaaaa aaacaaatct acaatttgtt tctacagctg atatgcaaaa
       61 agatgcgaaa gatttaagcc ttaaattttt accatattct ttattaatat caattttatt
      121 ttctgctatt atttctttaa tttatgcaaa atcaataaaa aataatatac aagaaataaa
      181 aattgttact gataaaatga tgaaacttga taaaaaaatg agtttaaaag ttagctctaa
      241 tgatgaagtt ggagaattaa aacaacaaat taatgatttg tattctactt tattaagaac
      301 aattgacgat ttggaattta aaaataaaga aattttaaaa ttagaaaagt taaaatatga
      361 cttttttaaa ggtgcatccc atgaacttaa aacccctctt gctagtctta aaataatact
      421 ggaaaacatg aaatacaata ttggaaaata taaagaaaga gatatttata ttaatgaatg
      481 tattgaaatc gttgatagtt taacaaaaaa tatttctcaa atattatcag ttcattctat
      541 agaaaatttg aataatgatg aagaatattt gaaaataaat gatacattag aagacatatt
      601 aaaaaagtat gaaatattag caaatcaaaa gaaaataact gtcaataatt atatattaga
      661 cgagaatgtt tatataggaa aaacagcttt aaagattatt ctatctaatt tgattagtaa
      721 tgcggtaaaa tatactgatg taaatggggt aattaatatt gggatagtta atgattggtt
      781 atatatagag aattcatacg gtaacaataa aatttcgaat atggataaaa tatttgatgt
      841 taaatttgat ttaaataagg aaaatagtaa tggattaggt ttatatattg taagtaatat
      901 tctgaataat tacaatatag aatataaagt attgcaggat gaagaatttt ttatctttaa
      961 aataaaaata ttttcttaat taagatataa ttttgcaata gttattgaaa aata
//
LOCUS       DAPBAB010000203         1008 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPBAB010000203 DAPBAB010000000
VERSION     DAPBAB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.221"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            63..1008
                     /locus_tag="RD444_003057"
                     /pseudo
     CDS             63..1008
                     /locus_tag="RD444_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          316 a          201 c          200 g          291 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaag
//
LOCUS       DAPBAB010000204         1001 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPBAB010000204 DAPBAB010000000
VERSION     DAPBAB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.222"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..>1001)
                     /locus_tag="RD444_003058"
     rRNA            complement(<1..>1001)
                     /locus_tag="RD444_003058"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DAPBAB010000205          903 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPBAB010000205 DAPBAB010000000
VERSION     DAPBAB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.224"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            1..903
                     /locus_tag="RD444_003059"
                     /pseudo
     CDS             1..903
                     /locus_tag="RD444_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          330 a          177 c          191 g          205 t
ORIGIN      
        1 caagtgaagc gctggatcaa tgcctatcaa gaatttggtg aagagggtct agtaagaaag
       61 cgacaaaaga aaaaatattc tgttcaattt aagctggatg cgatagagtt atatctaaca
      121 agtgagctgt cctatcgtga agttgccaac acacttaaca tgaacaaccc taatttgatc
      181 gctagttgga tgcgtcaatt ccgtgagggt gggatcgatg gactctcaaa aacgaaagga
      241 tgtccaccta tcttgtctaa gaaaaatgaa cccaaaaata agaaaaaatc tacaactaaa
      301 gcaacgtcta aagaacgtga acgcatcgag gagttggaga aacgagtccg ttcccttcaa
      361 attgaaaatg cctttttaaa agaattgagg aagctgcgaa aacaggaagc ccaacaacga
      421 cgaatgaagc aatcgcacga atcatcgcaa gcctccgagg accgtttaaa ttagttgagc
      481 ttctagaaac tttaaagttc cctaaggcaa cctttatgta ctggcaaaaa cgattggacc
      541 gtaaaaatcc agatcaagag attgaagcgg aaatgcttaa aattcgggag aagcacaagg
      601 attatggctg tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa
      661 agaaagttca gcgcctgatc aagaagctag gaataaaagt aacgacatac acacgtaaat
      721 cacggcgcta caattcttat cgaggacaag tcggagcagt agcccaaaat cggatccatc
      781 gtcgtttcta caccagtatc tgccatcaga aaatcacgac ggatacaact gaatttaaat
      841 accatgaagt ggatgccaaa ggcattatcc gtcaaaagaa attgtatcta gatcctttta
      901 tgg
//
LOCUS       DAPBAB010000206          890 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPBAB010000206 DAPBAB010000000
VERSION     DAPBAB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.225"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..888
                     /locus_tag="RD444_003060"
     CDS             <1..888
                     /locus_tag="RD444_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDT7550326.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAPBAB010000207          876 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPBAB010000207 DAPBAB010000000
VERSION     DAPBAB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.226"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..434)
                     /locus_tag="RD444_003061"
     CDS             complement(<1..434)
                     /locus_tag="RD444_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7550327.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="RD444_003062"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="RD444_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDT7550328.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DAPBAB010000208          874 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPBAB010000208 DAPBAB010000000
VERSION     DAPBAB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.227"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..142
                     /locus_tag="RD444_003063"
     CDS             <1..142
                     /locus_tag="RD444_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7550329.1"
                     /translation="KEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMF
                     TE"
BASE COUNT          312 a          151 c          110 g          301 t
ORIGIN      
        1 aaaagagcag tttcaaaatg aagaatcact agagcgtttc ctagtatcca tctttgatac
       61 atacaatcaa aaattcttaa atagaagcca taaaggcttc caacaagtga cggatacatt
      121 agcttcaatg tttactgagt aacccattat tttgcagaag gacgagttat ttacacaaaa
      181 ttattgacgc tccctccaaa ttacttcatc aaaacattga tataattttt attatatcag
      241 tatttattct tgatacataa atgagaaaat aaatacttta aatctattga ctacaagtag
      301 tcattaagtc ttcataagat gtacaatatg acattaaaaa aataaaagct tacgaccatc
      361 cgttaaatct gacactaaat aatggactgc tttcttttgt attagcaggt tacgttgact
      421 atgaatattc accagattat ttggagaaac ctcttcaaca cgagaacaat gatattaatt
      481 ttagatttgt ctacgagtca aaaagtaaaa catggttagt tgatcaatta agcacgataa
      541 acacaagctt ccatgaatta cctaaacctg aaaatgaagt tgagatggtc attaaaatct
      601 tctaaaaaag aagcgttgaa ggggaaatat tcctttcaac gctctttttt atatgcttag
      661 aaacataaat aaattgtata ctttcttttt agcccctatt acttttcacg aaaaatatct
      721 ttttttcgac gctagcgttt ctctacttct aatactgtac acagtttttg tattattgaa
      781 aaattactca ttatttagca ttaatccaca tcgttttatc aatcgacctt catactaaac
      841 tactttaaag tcatcttcct tctcgttttt catt
//
LOCUS       DAPBAB010000209          835 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPBAB010000209 DAPBAB010000000
VERSION     DAPBAB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.228"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(52..507)
                     /gene="tnpA"
                     /locus_tag="RD444_003064"
     CDS             complement(52..507)
                     /gene="tnpA"
                     /locus_tag="RD444_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDT7550330.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          237 a          159 c          140 g          299 t
ORIGIN      
        1 ggggattttt aaaagaccgc caactgctca tcttcactca cttacttctc tttatttttt
       61 aaatggttct acgtactctc ttttacttat aatatatcga atgcgatcat cagcttcttg
      121 ttcacggatg tatttttcat ttgtcttttg attcaatccg actgtactca catagtatct
      181 cttcgaccaa aaggatttgt ttccgtattt atatttgaga tttgcgtgct gctcgtggat
      241 tagtaccgca ctacgacctt tcagataccc cataaaactg gatatacata tttttggtgg
      301 tactcgaaca agcatgtgaa tatgatctag cattgcatgt gcttctatga tttccacatc
      361 tttcatttcg cacaacttcc taagtatttt tccaagatct tttcgtaatt gcccatagat
      421 tacttttcgt tgatattttg gtgtaaaaat aatatgatac ttacaattcc aacgagtata
      481 tgctaaactt ttatcgtcat ttgacatgaa aaaactcctt tcgatctgat gagcggttgg
      541 tagccactca ctagtatatc gaaaggagtt tttatcgcta aagatacttc ttccacgcgt
      601 agaaacgcgc ggttttcttg tttcgcacgc tttacgcaca aaactgactg aagtcattaa
      661 gaaaataaaa gggtctaaaa cttttaagtt ttgtaccgac ccccaaaagt tagaccaaaa
      721 aatctaactt ttggaggtcg gtatttttta tggctaagta tacttttgaa cttaaattaa
      781 agatcgttca tgattacttg gatggtaaag gcggaagtga ttatctggca aagaa
//
LOCUS       DAPBAB010000210          832 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPBAB010000210 DAPBAB010000000
VERSION     DAPBAB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.229"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..693
                     /locus_tag="RD444_003065"
     CDS             <1..693
                     /locus_tag="RD444_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550331.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          304 a          132 c          154 g          242 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttcaa gcgttaagaa ccgtcaggtt ta
//
LOCUS       DAPBAB010000211          813 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPBAB010000211 DAPBAB010000000
VERSION     DAPBAB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.230"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            143..400
                     /locus_tag="RD444_003066"
     CDS             143..400
                     /locus_tag="RD444_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550332.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..>813)
                     /locus_tag="RD444_003067"
     CDS             complement(632..>813)
                     /locus_tag="RD444_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDT7550333.1"
                     /translation="QDKVQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQ
                     TELNDIISDFNQVNN"
BASE COUNT          312 a           98 c          108 g          295 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gat
//
LOCUS       DAPBAB010000212          802 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPBAB010000212 DAPBAB010000000
VERSION     DAPBAB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.231"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..693
                     /locus_tag="RD444_003068"
     CDS             <1..693
                     /locus_tag="RD444_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550334.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          286 a          141 c          147 g          228 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtggacataa agacggcaag aaatctctcg aaattcgaga atggacttgc
      601 cctatttgtc atactcatca tgaccgagat atcaacgcta gtatcaatat cctgaccgaa
      661 ggtctaaggc tacactctat gggattggct tagatcaaat aaacaagaac cgtaggaact
      721 acggggatag cttggtaaat aagaggcacc tctgttagta aagtaatacg ctatcaagta
      781 tgctctcttc ccaagaagct cc
//
LOCUS       DAPBAB010000213          796 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPBAB010000213 DAPBAB010000000
VERSION     DAPBAB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.232"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            19..>796
                     /locus_tag="RD444_003069"
     CDS             19..>796
                     /locus_tag="RD444_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDT7550335.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAH"
BASE COUNT          284 a          149 c          137 g          226 t
ORIGIN      
        1 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
       61 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      121 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      181 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      241 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      301 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      361 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      421 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      481 gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg tcaaaccgta
      541 tccaaagaag cgatttatat tgccattggt atacgagaag acggcactaa agaagtactg
      601 agttatgcga ttgctccaac tgaatcaaca tacgtttgga atgagctgct acaggatatt
      661 aactccagag gagttcaaga agtcttgctt tttattacgg acggcttaaa aggcatgaaa
      721 gatactatcc atcaaattta tcctaaagca aaatatcagc attgttgtat ccatgtatct
      781 cgtaacatcg ctcata
//
LOCUS       DAPBAB010000214          791 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPBAB010000214 DAPBAB010000000
VERSION     DAPBAB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.233"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..782
                     /locus_tag="RD444_003070"
     CDS             <1..782
                     /locus_tag="RD444_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7550336.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DAPBAB010000215          789 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPBAB010000215 DAPBAB010000000
VERSION     DAPBAB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.234"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(150..>789)
                     /locus_tag="RD444_003071"
     CDS             complement(150..>789)
                     /locus_tag="RD444_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDT7550337.1"
                     /translation="GVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFG
                     VKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKA
                     SELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLE
                     RLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          238 a          181 c          137 g          233 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacct
//
LOCUS       DAPBAB010000216          761 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPBAB010000216 DAPBAB010000000
VERSION     DAPBAB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.235"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            656..>761
                     /locus_tag="RD444_003072"
     rRNA            656..>761
                     /locus_tag="RD444_003072"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          313 a          124 c          134 g          190 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actctcgatg aagtagacct ttgaaaactg aacaaagtaa
      541 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag
      601 caagcaatat gctagcaaac aaattgagct taacaatcgc aagattgttc gaacttttta
      661 tgagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca tgcaagtcga
      721 acgcttcttt ttccaccgga gcttgctcca ccggaaaaag a
//
LOCUS       DAPBAB010000217          743 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPBAB010000217 DAPBAB010000000
VERSION     DAPBAB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.236"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            145..630
                     /gene="ssb"
                     /locus_tag="RD444_003073"
     CDS             145..630
                     /gene="ssb"
                     /locus_tag="RD444_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7550338.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT          244 a          151 c          151 g          197 t
ORIGIN      
        1 ctattttgcc aaagatattt ctttcgaatc taccttaact tctgttgatt cgctgatgca
       61 atcaattgtc gagcaagagg aagtagaacg aaaacaaaat tttaagagat cacaacaaat
      121 gaatcaagaa atggagagat aaagatgatc aataatgtaa ctttagtagg tcgattaacc
      181 aaagatcccg atttacgtta caccgcaagt ggcactgcag tgaccacttt taccttagcc
      241 gttaaccgta attttactaa tcaaagtggc aatcgagaag ctgactttat caattgtgtg
      301 atttggcgca aaccagctga aacgatagcg acattagcta aaaaaggcag cttgatcggc
      361 gtcgtcggac ggattcaaac gcggacttat gacaaccagc aaggacagcg tgtctatgtg
      421 actgaagttg tggccgataa tttccaacta ttagaaagta aagcagcgac agaaagccgt
      481 atgcacgctg atcaatcgtc cagcagtcca agccctacca cctttgaaca acgggataca
      541 gcgacgccta acaacaatgg attgaacgct tcacagaatc catttggtgg acaatcaatc
      601 gatattagcg atgatgatct accgttctaa gtagcatttt ctattggttg catttgctac
      661 ggtttatgtt ataatttaga caacaaaaag aattttcaat ctataaccaa aaaccactag
      721 ctggcgggct agtggctttc ttt
//
LOCUS       DAPBAB010000218          713 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPBAB010000218 DAPBAB010000000
VERSION     DAPBAB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.238"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            218..493
                     /locus_tag="RD444_003074"
     CDS             218..493
                     /locus_tag="RD444_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550339.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            535..>713
                     /locus_tag="RD444_003075"
     CDS             535..>713
                     /locus_tag="RD444_003075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550340.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNA"
BASE COUNT          287 a           78 c          130 g          218 t
ORIGIN      
        1 tatcactttc tcacgaaagg tgttaggtag gctgtgccta ccacgtttct aagaaagata
       61 aatgatatca aaattttgga tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg
      121 gcagtaaaat aagcgtttgt gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct
      181 acacatatcc ctacacataa taaatttgga ggaaaatatg gacttattaa ttagaaatat
      241 tgaagaaaag tatattaata aaattgacaa acgttgtgaa gaacttacgg tcaagactgg
      301 caaaaaatgg agtcgaaatc aatacctaaa agttttgatc gaaaatgatt ttgatcacgc
      361 tcttctgaat tataaaaaag accaatttga tcggttgtta gaaaaatttg tggatattca
      421 aacttataat acgaaaactt tagaagaata tattgcaaca aataatcaat taattggttt
      481 gttaattgaa taataaaaat agtattgtga atttataaaa ggaggaaatc aattttggca
      541 aaagcaacaa gaaaacatgt atatctttac gatatgcata tcgaagtact tgatgaaata
      601 atttctactc atgaagatat atctaattat agtgaagcag tcagacattt gtgtatgatg
      661 tatgatccaa aaatcgaaga aaaaataaat ccaattcaaa ttaaattgaa tgc
//
LOCUS       DAPBAB010000219          712 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPBAB010000219 DAPBAB010000000
VERSION     DAPBAB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.239"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(103..552)
                     /locus_tag="RD444_003076"
                     /pseudo
     CDS             complement(103..552)
                     /locus_tag="RD444_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611666.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(607..>712)
                     /locus_tag="RD444_003077"
     CDS             complement(607..>712)
                     /locus_tag="RD444_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7550341.1"
                     /translation="MKAIRGLYKKTRKEGALFGFSVCTEIKVLLGIPA"
BASE COUNT          218 a          117 c          116 g          261 t
ORIGIN      
        1 aacctataat tcaaatagga agccttgcgt attatttaag atggttctgt tgcaaagtta
       61 aaaaattaat aaagagtttc caattaattt ttgattttat gcttagatac tcattaatgt
      121 attcatctca tgaatcactg aaaagccaaa gagacttttg tctctttggc taattttata
      181 taaagcgtgt atagtttcaa ttcctttaat tgttgaggaa tcagtcctta tggactgata
      241 taatttgtgc cttttcttga tggatcgatg atcctgttca atgatattat tcaagtattt
      301 atttgttcgg tgttcagtct tagaaaataa tccttcagaa tgcaattgat taaacgcact
      361 acttagtgaa ggggctttat cagtgacaag aactttaggt tctccaaatt gtttgaccaa
      421 tcgtttaaag aaagcataag ctgcttgttt atcacgcttt tttcgtaacc aaatatctaa
      481 tgttatcccc gaagaatcaa tggatcgata caaatagtgc cactttcctt ttaccttaat
      541 ataagtttca tcggttctgt tgcaaagttt taaataaaga ataaaatctc ttacggtatc
      601 tatgatttaa gctgggattc ccaataatac cttgatttca gtacagaccg aaaacccgaa
      661 gagagcgcct tcttttcggg ttttcttata taatcctcga atggctttca tg
//
LOCUS       DAPBAB010000220          704 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPBAB010000220 DAPBAB010000000
VERSION     DAPBAB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.240"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            62..654
                     /locus_tag="RD444_003078"
                     /pseudo
     CDS             62..654
                     /locus_tag="RD444_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          262 a          113 c          130 g          199 t
ORIGIN      
        1 ctagttttct aatgtatgat tagggtgtgt tttaaatagt tcgagaaagt agggtaaatt
       61 tttgaaaatt gcttatattc gagtttcgtc gattgatcaa aatgaacaaa ggcaacttga
      121 agaaatgaag aagttcggcg cagaaaaaat atttattgag aaacaatctg gagcaacgat
      181 tactcatcga ccaattttcc aagaagcttt agaatttgtc tgagaacaag atatttttat
      241 cgtggaagcc atcgatcgac ttggaagaaa ttatgacgag atcattgaat cggttaatta
      301 tctaaaaaga aaaaatgtac aacttatcat cactagttta ccaatcatgg ctgaagcaat
      361 cctctcttgg ataagttcat caaggattta atcattcaga tcctggccat gattgccgaa
      421 caggaacgta cagaatctaa acgacgccag gcacaaggaa ttaagattgc aaaagcaaat
      481 ggtgtttata aaggacgtcc taagttatac agcgcagaca caaaagatcc ccaacgtcgc
      541 cttgtttata aaagcatcgt tgaggatcta aatcagggta ttgctatttc aaaaactgca
      601 aaaaattata acatcactag acaaacagtt tatagaataa aaaacgattt gtaagtatgg
      661 taaggaaaca gctctcttta ttctcagata tctaaaacta caat
//
LOCUS       DAPBAB010000221          681 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPBAB010000221 DAPBAB010000000
VERSION     DAPBAB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.241"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>681
                     /locus_tag="RD444_003079"
     CDS             <1..>681
                     /locus_tag="RD444_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HDT7550342.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGT"
BASE COUNT          244 a          113 c          134 g          190 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta c
//
LOCUS       DAPBAB010000222          670 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPBAB010000222 DAPBAB010000000
VERSION     DAPBAB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.242"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..640)
                     /locus_tag="RD444_003080"
     CDS             complement(<1..640)
                     /locus_tag="RD444_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDT7550343.1"
                     /translation="MDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKN
                     STANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFG
                     EAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQ
                     VPKEYKVIYEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQL"
BASE COUNT          183 a          148 c          108 g          231 t
ORIGIN      
        1 aaagctgctg accaaaagac gcatttactt tgtgagcgga aaccagttgg ttcgttaatt
       61 gcagattgtt ttctgtcttg tgtatggcca taattgcttc aggttcaact ttcatttctt
      121 ttgcaacttc ttcataaatg actttgtatt cttttggcac ttgccaatca ttagcgattc
      181 ctcgttcaca acgtactttc tgattcgtaa atggatcaac tgcgcccatt aattgttctt
      241 tttcgtagaa aatatgtcct gcacggtcat tatccacaca aatctcaatg gattgaggaa
      301 aggcttgttt ctttctcaat tcagatactg cctcaccaaa ataatgactg atcacatggt
      361 gttttaaccc ttccatagag actaaccacg tatcgtttaa ctgatcacga ttcagtgcag
      421 catagctaag taaatcaata ctactctcaa aaaattttaa ctgtttaggg tcaccgatct
      481 ttaggttaaa tccatgattc gctgtcgaat ttttatctat atgcttatat gtgcctctct
      541 caccaaaacg ttcataatca ataaatgttc cttgataact cgcccctact gtctcttttg
      601 tctgtggatc tttccataaa aaggcgaccg catgatccaa acgatcgtct cgaactaaga
      661 aattttgccg
//
LOCUS       DAPBAB010000223          660 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPBAB010000223 DAPBAB010000000
VERSION     DAPBAB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.243"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            18..641
                     /locus_tag="RD444_003081"
     CDS             18..641
                     /locus_tag="RD444_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550344.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          263 a           89 c          128 g          180 t
ORIGIN      
        1 gataggaatg atggcaaatg tatttagctt atttaagagt ttcttccgac gatcaaagtt
       61 tagctcggca acataagcta attgaaaatt ggtcagtcaa aaataatatt gctgaagacg
      121 atttaaaaat tttcgaggaa aaagtctctg gaaaaaatat tgaaaacaga ccagagctgc
      181 aagagcttat ttctttcata cgtgaacgag atacgattat tattcctagt cttgatcgat
      241 tagcaagaaa tagtaaagat acaaaagact tacttcaatt gtttcgtagt aaaggtgctt
      301 ctattgaaat attagaccta ccgtcgttta atggggtaac tgaccctgca ttaagagact
      361 tgctaacaaa tttagtcata gaagtgttta gctatgttgc tgaaaacgaa cggaaaaaaa
      421 tcaaagagcg tcaaaaacaa ggcattgaaa ttgcaaagaa aaatggtcgt tatactggta
      481 aaaagattca ataccatgcc aatgcaacag gaaaaaacaa attgatttac gatgaaattg
      541 ttgatggtct gaagaaaaaa gaaactattt ctaaaatagc aaagaacgca ggagttacaa
      601 gaaaaaccgt ttatcgagta aaaagagatt taggaatgta atatgttcca gttagaaaga
//
LOCUS       DAPBAB010000224          640 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPBAB010000224 DAPBAB010000000
VERSION     DAPBAB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.244"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..283
                     /locus_tag="RD444_003082"
     CDS             <1..283
                     /locus_tag="RD444_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7550345.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DAPBAB010000225          592 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPBAB010000225 DAPBAB010000000
VERSION     DAPBAB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.245"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            51..>592
                     /locus_tag="RD444_003083"
     CDS             51..>592
                     /locus_tag="RD444_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDT7550346.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          217 a           94 c          117 g          164 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cg
//
LOCUS       DAPBAB010000226          570 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPBAB010000226 DAPBAB010000000
VERSION     DAPBAB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.246"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            27..>570
                     /locus_tag="RD444_003084"
     CDS             27..>570
                     /locus_tag="RD444_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7550347.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLV"
BASE COUNT          194 a          104 c          111 g          161 t
ORIGIN      
        1 caaagaatta taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg
       61 taataataga gtcttatttc aaaatgaatc aatctgttgc taaaactgcc cattgcttga
      121 atcgttcaag acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct
      181 tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt
      241 tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      301 tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt
      361 tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg
      421 gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg
      481 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga
      541 aacataaaag tgctgtaatt accttagttg
//
LOCUS       DAPBAB010000227          566 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPBAB010000227 DAPBAB010000000
VERSION     DAPBAB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.247"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..341)
                     /locus_tag="RD444_003085"
     CDS             complement(<1..341)
                     /locus_tag="RD444_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7550348.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWI"
BASE COUNT          176 a          110 c           77 g          203 t
ORIGIN      
        1 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
       61 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      121 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      181 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      241 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      301 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      361 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      421 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      481 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      541 agagaatact cgcgacttta gtcatg
//
LOCUS       DAPBAB010000228          556 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPBAB010000228 DAPBAB010000000
VERSION     DAPBAB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.248"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..163
                     /locus_tag="RD444_003086"
     CDS             <1..163
                     /locus_tag="RD444_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7550349.1"
                     /translation="GLPKSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYV
                     QEAFYSSLI"
BASE COUNT          229 a           79 c           64 g          184 t
ORIGIN      
        1 tggactgccg aaatcaatgg attttagaga agtgaatcag acatttattt ccagtgtcag
       61 caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt
      121 tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca tacaaacaaa
      181 aataaataaa gtcggatttt tctgataatt caacgcaaaa aaacgaacaa tttttcaata
      241 aatcagtctt ctaaaataga taacctaaaa aaaacgaaca aacaaataaa atgactgtcc
      301 ttcattcact aaacatattt ttatttttta tattgttaaa aattcacaat tattttttaa
      361 tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat
      421 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
      481 tgtgcaaaaa aatacaaggt ggaggctcta ctatgattga agaatatatt gagaaaaata
      541 ttttaagaca gctgtt
//
LOCUS       DAPBAB010000229          536 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPBAB010000229 DAPBAB010000000
VERSION     DAPBAB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.249"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>536
                     /locus_tag="RD444_003087"
     CDS             <1..>536
                     /locus_tag="RD444_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550350.1"
                     /translation="IVKQTIHVQKIISSEQLDNQGQKLEKQEGVNGAKFSVYDVSDIL
                     QKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQTGVFEFSVEVQANQK
                     QAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEASQPKEEVKKIVST
                     GVKKNFFENCWDFVTHLFS"
BASE COUNT          202 a           84 c           97 g          153 t
ORIGIN      
        1 atagtcaaac agaccattca cgttcaaaaa attatttctt ccgaacaatt agacaaccag
       61 gggcaaaagc ttgaaaaaca agaaggagta aatggcgcga agttttctgt ttatgatgta
      121 tcggacattt tacaaaagat ggatgttaag gatctcacta ctgaccaaat tgaatcccaa
      181 ttaaaagatc gagtgaaaaa actatccagt gatcaactga agctaatttc taatggagaa
      241 acaaagacaa ttgatcaaca aacaggtgtc tttgaattct cggtagaagt acaggcaaat
      301 cagaaacaag cttattatat tgtcaatgaa tcaagtcctg aaaacatttc taatagcgag
      361 gatattttgc tattaacacc tgtttctgat aaaaatggtg aattcttaaa ggatgtgtgg
      421 atttatccta aatcagaagc ttcgcaacct aaagaagaag taaaaaaaat cgtatctact
      481 ggcgtaaaga aaaacttctt tgagaattgt tgggattttg ttactcactt gttttc
//
LOCUS       DAPBAB010000230          528 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPBAB010000230 DAPBAB010000000
VERSION     DAPBAB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.250"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(5..90)
                     /locus_tag="RD444_003088"
     tRNA            complement(5..90)
                     /locus_tag="RD444_003088"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="RD444_003089"
     tRNA            complement(107..178)
                     /locus_tag="RD444_003089"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="RD444_003090"
     tRNA            complement(190..262)
                     /locus_tag="RD444_003090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="RD444_003091"
     tRNA            complement(280..361)
                     /locus_tag="RD444_003091"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="RD444_003092"
     tRNA            complement(369..441)
                     /locus_tag="RD444_003092"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="RD444_003093"
     tRNA            complement(445..517)
                     /locus_tag="RD444_003093"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAPBAB010000231          517 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPBAB010000231 DAPBAB010000000
VERSION     DAPBAB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.251"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
BASE COUNT          169 a           87 c          117 g          144 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctacta
//
LOCUS       DAPBAB010000232          516 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPBAB010000232 DAPBAB010000000
VERSION     DAPBAB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.252"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            156..485
                     /locus_tag="RD444_003094"
     CDS             156..485
                     /locus_tag="RD444_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7550351.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          194 a           61 c           99 g          162 t
ORIGIN      
        1 ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt cagcaaggac
       61 gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa tgtatttact
      121 aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca agaaaacaag
      181 gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga gtcgaagttg
      241 aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa aaagaatttt
      301 atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat aaagatgaga
      361 ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga gatattgctg
      421 aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca gaaattggct
      481 tatgaaatcg ttccttcaaa acatgtcatt aagtat
//
LOCUS       DAPBAB010000233          507 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPBAB010000233 DAPBAB010000000
VERSION     DAPBAB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.253"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..200)
                     /locus_tag="RD444_003095"
     CDS             complement(<1..200)
                     /locus_tag="RD444_003095"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550352.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRP"
     gene            complement(311..>507)
                     /locus_tag="RD444_003096"
     CDS             complement(311..>507)
                     /locus_tag="RD444_003096"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550353.1"
                     /translation="SVRGLAKEYGLSEATIYKWKNLYLPDQSTGLTGKEVAELRKENA
                     RLNEELEILKKAAAIFSRKT"
BASE COUNT          151 a           91 c           73 g          192 t
ORIGIN      
        1 tgaggtctat attttttaac tacaatcgac cttaaattta attgtttcat tagcttttgg
       61 acatgtttaa gagatactga aatcccttct tttaataaaa cttgatggat ctttgtggca
      121 ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct tctttttcaa cttattattt
      181 ctgatttctg tagctgtcaa cggtttattc ttataaaaat aatagacact tctagggatt
      241 tctaataacc gacacaacaa agaaacagtg taatccttac accatttttc aataaattga
      301 acaagcgaat ttaggttttc cgagagaata tggctgcggc tttttttaag atttcaagtt
      361 cctcatttaa acgagcattt tcttttctca gttcagctac ttcttttcca gtcagtcctg
      421 tggactgatc aggtaaatat aaattcttcc atttgtaaat cgttgcttca gataagccat
      481 attctttcgc taaccctcga acagaac
//
LOCUS       DAPBAB010000234          500 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPBAB010000234 DAPBAB010000000
VERSION     DAPBAB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.254"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..354
                     /locus_tag="RD444_003097"
     CDS             <1..354
                     /locus_tag="RD444_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550354.1"
                     /translation="VLETLSEEANLPNILGDKGYVSHKIHEKLALKGITVLVPSRKNM
                     DKLGKIDHRLLTRQRKAVETVFSSLERLGFQNFKSRSIQGLESKFESILLSYSILLSR
                     AQQHFEGTLRYSLGY"
BASE COUNT          185 a           75 c           86 g          154 t
ORIGIN      
        1 gttttagaga ctttatcgga agaagcaaac cttccaaata tccttggaga taaaggctat
       61 gtcagccata aaatccatga aaagcttgcc cttaagggca ttacagtctt agttccgtca
      121 cgaaaaaaca tggataaact agggaagata gaccatcgat tacttactag acaaagaaaa
      181 gcagtagaaa ctgttttttc ttctctcgaa agactaggtt ttcaaaattt caaatcacgt
      241 tctattcaag ggctagaaag taagtttgaa agcatattat tatcgtacag tattctttta
      301 agccgagcac agcaacattt tgaagggact ttgagatatt ctttagggta ttaaaattaa
      361 ctagcaccac gggtatacta ctatatttta ggtttcatat tatataaatt aataatatac
      421 taccatttgt tggtagtata atttaaagag agtgtaaaat attttgtgta aatgaaaaaa
      481 tccatacaaa aaaggaagtc
//
LOCUS       DAPBAB010000235          478 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPBAB010000235 DAPBAB010000000
VERSION     DAPBAB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.255"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..413
                     /locus_tag="RD444_003098"
     CDS             <1..413
                     /locus_tag="RD444_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550355.1"
                     /translation="ITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYNHNHRGFT
                     CYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVDAMNVFV
                     RAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT          167 a           61 c           81 g          169 t
ORIGIN      
        1 ttattacttg tctatttttg ttcggtatga ttgttggagc aagtactccg ttaaatctgt
       61 ttccttttag tgctagaggt ataaaagtgg aagctgcaag ttcacgatat aatcataatc
      121 atcgtggatt tacttgctat aaaatgaact attatgttac taataaaatg tgtagagatt
      181 tgaaaaaaaa ttataataag ctaaagactc ctgctcaaat tgcatctttt attcctattg
      241 gtggagttgg aacatgggtt attacaaatg cttttagtag tgctgttgat gcaatgaatg
      301 tctttgtaag agctgcaaat caaggaaaag gtgtacaatt aacatataat gctcacttta
      361 gcaatacaac atcatatcaa tacaatgatt acgctcgtta tgtaataaaa taatcagaaa
      421 gtgaaactat ctcatgaata aaaagatttt agctgtaatt atttcaataa tagtttta
//
LOCUS       DAPBAB010000236          473 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPBAB010000236 DAPBAB010000000
VERSION     DAPBAB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.256"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>473
                     /locus_tag="RD444_003099"
     CDS             <1..>473
                     /locus_tag="RD444_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7550356.1"
                     /translation="IIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSS
                     NLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKK
                     SYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISH"
BASE COUNT          153 a           86 c           85 g          149 t
ORIGIN      
        1 tgattattgc ttgtgttata tggggcataa ttaatggcta tactagccaa agagccacgt
       61 atagagcggt ttgttccgtc ttatttccta atggtgactt tcctagtagg agtcgattca
      121 ctcgcttaag ttcaaactta gcttacacta tcaagattat tcgatacttt ttcatcaaga
      181 agctcaccaa aggtgaacta gtcggaatta tagatagttt tcctagtcca ttatgtaaac
      241 cagttagaaa tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa
      301 caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc
      361 cgattactta ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat
      421 cggaagaagc gaatcttccc aatatcctag gggacaaagg atatattagc cat
//
LOCUS       DAPBAB010000237          471 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPBAB010000237 DAPBAB010000000
VERSION     DAPBAB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.257"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..384
                     /locus_tag="RD444_003100"
     CDS             <1..384
                     /locus_tag="RD444_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7550357.1"
                     /translation="RTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDGE
                     LVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISITM
                     DTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          171 a           88 c           88 g          124 t
ORIGIN      
        1 cgaacgattt ttattggaca aaccttgcta gcaattctga aaaaacataa gaaacaacaa
       61 ttagaaaatc gaatgaagta tggaaagctc tacattgatt caaatgcagt atgtacgaag
      121 gaagacggtg agctagttac gccaagtgta gtgaaatgga atactaggag gatatcgaat
      181 gcactctccc tctcttttaa cttccattct ctcagacata ctcatgctac acttcttctc
      241 gaaaatggcg caaaaatgaa agaaatctct gaacgattgg gccacagcag aatttcaatt
      301 acgatggata cttactcgca tgtaacagat aagatgagaa atgaaacggt cgatatcatg
      361 gaaaatctta gaaagaattc ttgatttttg ccaccgaaaa aaaccaccgg tggcaaaccg
      421 gtggcaaatc catgtaaaca tggtttattt tttacactgt tttcaaacaa a
//
LOCUS       DAPBAB010000238          452 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPBAB010000238 DAPBAB010000000
VERSION     DAPBAB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.258"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(<1..209)
                     /locus_tag="RD444_003101"
     CDS             complement(<1..209)
                     /locus_tag="RD444_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDT7550358.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMY"
BASE COUNT          154 a           84 c           91 g          123 t
ORIGIN      
        1 ctgtacatgg acgttgactc catgccaatc acaatttgat caaagtggta gctgtcattg
       61 aattcaagaa ttgtttctcg tatgaatgaa gcaccttcga tgtcattggc aacagagatt
      121 tcagataaga tagacaattg attgtcgtct gttaaaaaac aaacatctag tttttcagag
      181 ctaacgtcaa taccgacaaa taatttcatg gaagaaggac ctcctttcga tataaatttt
      241 ggaaaatgct tcgatactgt gggatttccc agaaactcac tgtatagcca caacctcgcc
      301 taatagaata cggtgataaa agagctagaa gctgccttcc aaactactat ttgaaagtct
      361 agtccattat cgaaacagct aatgagttgg tagtgataac agtggttcgg gtaacagact
      421 aaacagggta gacaaacgct acaggaggaa at
//
LOCUS       DAPBAB010000239          451 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPBAB010000239 DAPBAB010000000
VERSION     DAPBAB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.259"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..361
                     /locus_tag="RD444_003102"
     CDS             <1..361
                     /locus_tag="RD444_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7550359.1"
                     /translation="SKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIF
                     TAKTPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVS
                     LLLLVLATFSYAVLRYI"
BASE COUNT          163 a           69 c           82 g          137 t
ORIGIN      
        1 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
       61 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
      121 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
      181 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
      241 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
      301 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
      361 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
      421 attgccatga ttgttatttt agtcggatgt a
//
LOCUS       DAPBAB010000240          447 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPBAB010000240 DAPBAB010000000
VERSION     DAPBAB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.260"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>447
                     /locus_tag="RD444_003103"
     CDS             <1..>447
                     /locus_tag="RD444_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7550360.1"
                     /translation="SGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMKYNGETYEL
                     VINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNGKDVNGRND
                     GKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIE"
BASE COUNT          192 a           74 c           86 g           95 t
ORIGIN      
        1 aaagtggtac aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac
       61 accaggtaaa atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg
      121 aaacttacga attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag
      181 ccaaaaatca gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg
      241 tttatttccc taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac
      301 gaaatgacgg taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat
      361 atctagtcac acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac
      421 aatttagtat tcaagatcca attgaag
//
LOCUS       DAPBAB010000241          440 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPBAB010000241 DAPBAB010000000
VERSION     DAPBAB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.261"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            11..>440
                     /locus_tag="RD444_003104"
     CDS             11..>440
                     /locus_tag="RD444_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7550361.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DAPBAB010000242          435 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPBAB010000242 DAPBAB010000000
VERSION     DAPBAB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.denovo.262"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..>435
                     /locus_tag="RD444_003105"
     CDS             <1..>435
                     /locus_tag="RD444_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7550362.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKN"
BASE COUNT          153 a           84 c           85 g          113 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaacc
//
LOCUS       DAPBAB010000243        20895 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.044, whole genome shotgun sequence.
ACCESSION   DAPBAB010000243 DAPBAB010000000
VERSION     DAPBAB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.044"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            267..1304
                     /locus_tag="RD444_003106"
     CDS             267..1304
                     /locus_tag="RD444_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7550363.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="RD444_003107"
     CDS             complement(1393..1716)
                     /locus_tag="RD444_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7550364.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="RD444_003108"
     CDS             complement(1703..2410)
                     /locus_tag="RD444_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7550365.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="RD444_003109"
     CDS             2496..2837
                     /locus_tag="RD444_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDT7550366.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="RD444_003110"
     CDS             complement(3008..3856)
                     /locus_tag="RD444_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550367.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="RD444_003111"
     CDS             4454..5317
                     /locus_tag="RD444_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7550368.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="RD444_003112"
     CDS             5417..5899
                     /locus_tag="RD444_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDT7550369.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="RD444_003113"
     CDS             complement(6162..6629)
                     /locus_tag="RD444_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDT7550370.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="RD444_003114"
     CDS             6752..7603
                     /locus_tag="RD444_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7550371.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="RD444_003115"
     CDS             complement(7854..9812)
                     /locus_tag="RD444_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7550372.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="RD444_003116"
     CDS             complement(9809..10681)
                     /locus_tag="RD444_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7550373.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="RD444_003117"
     CDS             complement(11023..11895)
                     /locus_tag="RD444_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7550374.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="RD444_003118"
     CDS             12023..12592
                     /locus_tag="RD444_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDT7550375.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="RD444_003119"
     CDS             12659..13396
                     /locus_tag="RD444_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7550376.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="RD444_003120"
     CDS             13627..14895
                     /locus_tag="RD444_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7550377.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="RD444_003121"
     CDS             15382..15846
                     /locus_tag="RD444_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7550378.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="RD444_003122"
     CDS             15830..16459
                     /locus_tag="RD444_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7550379.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr(C)"
                     /locus_tag="RD444_003123"
     CDS             16813..18291
                     /gene="msr(C)"
                     /locus_tag="RD444_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDT7550380.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="RD444_003124"
     CDS             18338..18784
                     /locus_tag="RD444_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDT7550381.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="RD444_003125"
     CDS             complement(18983..19498)
                     /locus_tag="RD444_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7550382.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="RD444_003126"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="RD444_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6644 a         3710 c         4318 g         6223 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctacaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc
    20881 ttctcatatt tttcg
//
LOCUS       DAPBAB010000244        20895 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.045, whole genome shotgun sequence.
ACCESSION   DAPBAB010000244 DAPBAB010000000
VERSION     DAPBAB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.045"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            267..1304
                     /locus_tag="RD444_003127"
     CDS             267..1304
                     /locus_tag="RD444_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7550383.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="RD444_003128"
     CDS             complement(1393..1716)
                     /locus_tag="RD444_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7550384.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="RD444_003129"
     CDS             complement(1703..2410)
                     /locus_tag="RD444_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7550385.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="RD444_003130"
     CDS             2496..2837
                     /locus_tag="RD444_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDT7550386.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="RD444_003131"
     CDS             complement(3008..3856)
                     /locus_tag="RD444_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550387.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="RD444_003132"
     CDS             4454..5317
                     /locus_tag="RD444_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7550388.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="RD444_003133"
     CDS             5417..5899
                     /locus_tag="RD444_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDT7550389.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="RD444_003134"
     CDS             complement(6162..6629)
                     /locus_tag="RD444_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDT7550390.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="RD444_003135"
     CDS             6752..7603
                     /locus_tag="RD444_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7550391.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="RD444_003136"
     CDS             complement(7854..9812)
                     /locus_tag="RD444_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7550392.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="RD444_003137"
     CDS             complement(9809..10681)
                     /locus_tag="RD444_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7550393.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="RD444_003138"
     CDS             complement(11023..11895)
                     /locus_tag="RD444_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7550394.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="RD444_003139"
     CDS             12023..12592
                     /locus_tag="RD444_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDT7550395.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="RD444_003140"
     CDS             12659..13396
                     /locus_tag="RD444_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7550396.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="RD444_003141"
     CDS             13627..14895
                     /locus_tag="RD444_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7550397.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="RD444_003142"
     CDS             15382..15846
                     /locus_tag="RD444_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7550398.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="RD444_003143"
     CDS             15830..16459
                     /locus_tag="RD444_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7550399.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr(C)"
                     /locus_tag="RD444_003144"
     CDS             16813..18291
                     /gene="msr(C)"
                     /locus_tag="RD444_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDT7550400.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="RD444_003145"
     CDS             18338..18784
                     /locus_tag="RD444_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDT7550401.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="RD444_003146"
     CDS             complement(18983..19498)
                     /locus_tag="RD444_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7550402.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="RD444_003147"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="RD444_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6643 a         3711 c         4318 g         6223 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc
    20881 ttctcatatt tttcg
//
LOCUS       DAPBAB010000245        16519 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.056, whole genome shotgun sequence.
ACCESSION   DAPBAB010000245 DAPBAB010000000
VERSION     DAPBAB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.056"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            128..1168
                     /locus_tag="RD444_003148"
     CDS             128..1168
                     /locus_tag="RD444_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7550403.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1266..1454
                     /locus_tag="RD444_003149"
     CDS             1266..1454
                     /locus_tag="RD444_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDT7550404.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1512..1865)
                     /locus_tag="RD444_003150"
     CDS             complement(1512..1865)
                     /locus_tag="RD444_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7550405.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2396..2818
                     /locus_tag="RD444_003151"
     CDS             2396..2818
                     /locus_tag="RD444_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7550406.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2815..3045
                     /locus_tag="RD444_003152"
     CDS             2815..3045
                     /locus_tag="RD444_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7550407.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3543..3743
                     /locus_tag="RD444_003153"
     CDS             3543..3743
                     /locus_tag="RD444_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7550408.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3770..4963
                     /locus_tag="RD444_003154"
     CDS             3770..4963
                     /locus_tag="RD444_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7550409.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5031..6596)
                     /gene="guaA"
                     /locus_tag="RD444_003155"
     CDS             complement(5031..6596)
                     /gene="guaA"
                     /locus_tag="RD444_003155"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDT7550410.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6655..7578)
                     /locus_tag="RD444_003156"
     CDS             complement(6655..7578)
                     /locus_tag="RD444_003156"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDT7550411.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7774..8379
                     /locus_tag="RD444_003157"
     CDS             7774..8379
                     /locus_tag="RD444_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7550412.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8687..9703
                     /locus_tag="RD444_003158"
     CDS             8687..9703
                     /locus_tag="RD444_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7550413.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9844..11145
                     /locus_tag="RD444_003159"
     CDS             9844..11145
                     /locus_tag="RD444_003159"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDT7550414.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11191..11853
                     /gene="deoC"
                     /locus_tag="RD444_003160"
     CDS             11191..11853
                     /gene="deoC"
                     /locus_tag="RD444_003160"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDT7550415.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11874..12272
                     /locus_tag="RD444_003161"
     CDS             11874..12272
                     /locus_tag="RD444_003161"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDT7550416.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12338..13420
                     /locus_tag="RD444_003162"
     CDS             12338..13420
                     /locus_tag="RD444_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7550417.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13632..15194
                     /locus_tag="RD444_003163"
     CDS             13632..15194
                     /locus_tag="RD444_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7550418.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15187..16320
                     /locus_tag="RD444_003164"
     CDS             15187..16320
                     /locus_tag="RD444_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7550419.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16320..>16519
                     /locus_tag="RD444_003165"
     CDS             16320..>16519
                     /locus_tag="RD444_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7550420.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMT"
BASE COUNT         5357 a         2967 c         3479 g         4716 t
ORIGIN      
        1 gtacattttg tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta
       61 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
      121 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
      181 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
      241 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
      301 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
      361 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
      421 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
      481 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
      541 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
      601 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
      661 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
      721 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
      781 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
      841 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
      901 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
      961 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
     1021 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
     1081 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
     1141 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc
     1201 ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga
     1261 ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac
     1321 gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg
     1381 aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga
     1441 cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg
     1501 aattttactg actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc
     1561 attaaggata gttctttgtc tgtaaagcta tccatgtatt tttctacctg taaccttcga
     1621 gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat caatagatac atggagtaat
     1681 gatacaagat catacagcac ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt
     1741 aaataacgtg ggtcaatttc aatcattgtt cccacttgtt cacgagtgag accccgtttc
     1801 attcgagcct ctttaatcgc taaaccaacg gctctaaaat cgtatttatc ttctttttta
     1861 cgcatactaa atcacctcta tacattttac tgttcctata taaccataaa caggtataga
     1921 aaaacatgtt atatagttta tgagttccta tagaacactg tggaatttat taatgcgatg
     1981 aatccaattt caaatagaaa ggtcgctgat tttatttacc tatacaactt tataaaattt
     2041 agtcatactc ctagaaagta gataaaattg gcgacttaat ggagggaaaa gtcgccatga
     2101 gaaagataat ctgtcatcta gttctcttaa ttaagttctt atcaaaaaaa cttatccacc
     2161 tgtactggat agatatgacc atcaatgtgg ttttgcagat tacatagata gaaaaatcaa
     2221 agcatatgaa cagagaaaac aatctgttta tgtgcttttt tggtcactta ataaatatct
     2281 tttaggattt ctcttcattt atgcaacaaa tcccccgttc gtgtcggggt ttagtatgaa
     2341 gggagataaa gaagcacatg ccactccctt tcacgacaga aagggggtga aaagcatgaa
     2401 accatcttct tttcaagaaa ccatagaaaa tcagtttgac tatatctgca aacaagttat
     2461 tgaagatgaa cgaaaagatt acttcaaaca tctagacaga ctatcagcaa aagaagtctc
     2521 gttttctgat atagataact accttgtcaa ctctttttct actgttgata cgtacgtcac
     2581 tgatttccaa ctatttaaat tatttggatt aagaatcaat gttgaaagtg atttattaag
     2641 tgaagccctg cgtcatcttt cagagaaaaa acggaacatt attttacttc attactttat
     2701 ggatatgagt gatgtggaga ttgcggagtt actaaatttg aatcgttcga ctgtttatcg
     2761 tcatcgaata agtggactag ccatgattaa agaattcatg aaggagtgta aagaatgaaa
     2821 acagcttatc caagggttcc cttccccctc attgtaaagg cgacagatgg cgacgtggaa
     2881 gcaattaatc agattgttaa acattatcga gggtatacct caaaacgttc attacgtcgc
     2941 atgacagacg aatatggaaa ttctcatatg gttattgatg aaaccctacg tggcagaatg
     3001 gaaacgaaac tcatctctaa gattttatcg ttcgaaatta aatagtccca ttttttcgtg
     3061 gaagcgtgtc gaatcgtatc cacgcttccc gatttgggag gtttgttatt ccatcaaagc
     3121 atattgtgtg ttcagtaggt tttgataggc taacaagcca aacgttcttt gacaaccaaa
     3181 taaaggcaat cgaatacgtt tcggtaagaa aagagctgat gaactaacac gccatgacct
     3241 acaaaattag tgagcgattc atgttagtaa ttcgaaagta atctttagca ggattgctgc
     3301 catgactttc ttatcgtgat aatgatactc ccgtatagcc atagtccgag cgttagaagc
     3361 gtcacaggca atgggtacgg ctacatgaga accatgcaga agtgaaattc ctgtgagctt
     3421 tgctagagct gttcgattgc taagatattc ttctcttgtc agtgtattat tataaaacta
     3481 tttgatataa ttataggtga atattggagt tttcctagcc tataattggg aaaggagtaa
     3541 taatgataac ggtcacaaaa aaggatttag ttgagctagg atatggaact tctttttcag
     3601 cagacatcat aaaaaaagca aaagaattga tgattacaaa agggcatatc tactatcaat
     3661 ctcgaaagct agatagagtc cctcgtgaag ctgttgaaga aattctagga attcggtttt
     3721 caaatgaaaa caggtctgag tagtttttgc acagtaagga gtaaaaacta tggttaaaga
     3781 cccaataaaa aaagcaaaga atggaaccta ttattttcgt gcaaatctag gttatgattc
     3841 taaagggaaa aaaattcaaa aatatcgtag tggattcact actaaaaaag aagcaagaga
     3901 agaatattcg aaactgctat tgatgaaacc agaagaatta agtgagaata aggataaaat
     3961 gacgtttaaa cattatatct ttgaaatttt ccttccatgg tataagacac aggtaaagct
     4021 cagaacatat gaaaatcgct taccaactat taagaagcat ttctcttatt ttgataaaat
     4081 ggctgtttcc gatattgagc ctattgatgt gcaaaattgg caactaaaat tatcaaagaa
     4141 gtgtaagtct tcttatgtaa gagctgttca aggactattt tcagttgcta tggatcgagc
     4201 aattgtcctt gggataacca ccacaaatcc atctaagatt attggcaatg tgaaaaagca
     4261 aaagtctaaa attgaatttt ggacaaaaga agaatttgaa aaagtaatct ctctaattta
     4321 caaagaagat tactatcaac actttttatt tatctctctg tggtttttat ttatgactgg
     4381 aatgcgaatt ggtgaagcta ctgccattca atgggaagat attgattttg actcaggagt
     4441 actaaccatc aacaaaacac tctattacaa gaatcaatcg aattatagat ttgttgaacc
     4501 aaaaacaaag gcgagcgttc gtcatattgt attagataaa tgcacattaa cttatttatc
     4561 cgaatggaag gaagtacaac aaaatctaat tcagacagac tttgtaatga gctataacgg
     4621 tattcctacc caaaaacata cactatcata tgctattgaa cgctatgcta agctggctgg
     4681 tatccatcga atcagaatcc atgggttacg gcattctcat gcttccttgt taatcagtat
     4741 gggagaaaat ccacttatca ttaaagatcg tctaggacac gaagatattg agacgacttt
     4801 aggaacgtat ggacatcttt atcctaacag taatttcgag gtagctaaca aattaaatgg
     4861 agccatttcc tttaaagaag caaaacaaaa ttatgatact tcaccaaaga atcaatttac
     4921 tgtagactac ttgcgaaaca aagaaatgaa aaaagtgcaa taaaagtgca gtaaaaagat
     4981 aagaagagcc agaaaccctt ggtataagag gatttctagc tctcctacaa ctattcccac
     5041 tccactgttg ctggtggttt agaagtgatg tcatacacga tacggttgac gtgggctact
     5101 tcgtttacga tacgtacaga gattttttgc aagacttccc atgggatacg ggcaaagtca
     5161 gcggtcattc cgtcgattga agttacagca cggataccga ctgtgtagtc gtaagtacgt
     5221 ccgtcaccca taacaccgac agagcggatc cctggaagaa cagtgaaata ttgccagata
     5281 tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga tagcatcaga ttcgcgtacg
     5341 atttctagct tgtcttcagt gatttcgcca agaacacgaa taccaagacc aggaccaggg
     5401 aatggctgac gccaaacgat tgaatcaggc atgcctaact gagtccctaa ttcacggact
     5461 tcgtctttga ataatgtgtt taatggttcg atcaattcaa attgcatatc ttctggcagt
     5521 ccacctacat tatggtgaga tttgatcgtc tgtgctgttt ctgtaccaga ttcgataacg
     5581 tcagtataca acgttccttg tgctaggaaa gagacgcctt cttcaccagc aagttttgtt
     5641 gcttcgtcat caaaaacata tacgaattca ttcccaatga ttttacgttt ttgttctggg
     5701 tcagatacac ctttcaactt gtctaagaag cgatctcttg catcgacttt gatgatgttc
     5761 aagccgaatt ttccgcctag actttccatt acttgttccg cttcgccttt gcgtagcaga
     5821 ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg ctttttgtaa aagaacccca
     5881 acgacactag aatcaacgcc acctgaaaga ccaagcaaga cttttttgtc accaacttgt
     5941 tcacggattt tttctacttg catatcgata aagttatcca ttgtccagtt accttcacag
     6001 tgacaaacat caaaggcaaa gtggcgcagc aattcgattc cgtattcgga atgacgtact
     6061 tctgcatgga attggatacc ataaaaatta cgtgttgtgt cttggataga agcaatcgga
     6121 caatctttgc ttgttccgac tgtttcaaag ccttcaggaa ctttagttac caagtcacca
     6181 tggctcatcc atactgtttg ttgttttggt gtttctttaa acaagacagc ttgatcgctt
     6241 gttacttcta acaaggcttt cccatattcg cggtttttag caggctctac ggaaccgcca
     6301 agtttgtacg tgatcaattg cataccatag caaatcccta atacaggaat acctaaattg
     6361 aagatctcag gatcgatccc aaatgcattc tcatcatata cactgtttgg tccgccagaa
     6421 agaatgattc cctttggatt catcgctttt acttcttctg cagtaatgcg gtggctcaat
     6481 aattcggaaa agactccaag atcacgaata cgtcttgtga tcaattggtt atactgacta
     6541 ccgtagtcta atacgataat cttttcaacg ttcgtcatat cggcaacgtt tgtcaaattt
     6601 attaccccta tccctttaaa ttttatctca aaatagtcaa actattttaa aatgctaata
     6661 ttttcgcatg aaaatcgaat cgatgatatg attttcagtt ttatggagaa ttatatcggc
     6721 tcttcctcgt gtaggcaaga tatattcctg taaatttttc aaattcacat ttttccatac
     6781 atcttttgcc attttaaagg cttcttcacg atctccgata gcaaaacgat aataataatt
     6841 ctctggattt tggaaagccg tatccagtaa cgcaccaaaa cgatccaaat accatttttc
     6901 gatcagcacg ggatcggcat cgacatagac agaaaaatca aaaaagtcgc tgacgtagat
     6961 ttgctgattc gcaggtaatt gaagcgtatt gatcccctca acgatcaaga tatcaggctg
     7021 ctggatcaat tcgtacttgt ctggaatgat atcatacaca tcatgtgagt aaacaggtac
     7081 ttttatctct tcctgaccag attttacacg gttcaagaaa tcgatcagca tctccatatc
     7141 ataactttca ggaaatcctt tacggtccat gattccacgc tcttttaata tacgatttgg
     7201 ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt cgtttgaagg tacgagacaa
     7261 aatcgtctgc agcagtctgg ctgttgtact ttttccaacg gctacacttc cggcgatccc
     7321 gataataaaa ggcggtactt ctacgtattt atgaagaaat aaacctttac tcaagaacaa
     7381 agattcaaat tctttcatat aaagataaat cagatgagca agaggtacat agatatctcg
     7441 aacatcctgc atggaaatgc ggtcattcaa acttttgata ttatctagtt catcttgtgt
     7501 caaaggtgct tgcccattac gataaaattc tcgccattct tctcttgcca cacggtagta
     7561 gttcattgga tcattcatat tagcctcctg cgttctccca tcaatggaac tattttaaca
     7621 caaaataaac gcaaacccca ccacttaaga gagattgccg atctttttgt ataatcttta
     7681 taatagaaag aaacggagtc ttattcttga cttgcgcacg gatacataaa aggatagact
     7741 gttgtcatga aagaaatgag aaggatggaa gacatgacaa atcattacta ttctgaacgc
     7801 ccagatacgg cgcacgattt tgaacaatgg gcttttgagt taaaaggcag aacctttcaa
     7861 tttgtaacag attctggtgt attttcacgt gaaacagtgg attatggttc acgtgtcctg
     7921 attgatgctt tcgattggga agagttacct gatgaaggaa agatcttaga tgtgggatgc
     7981 gggtatggac cgattgggtt agctttggca tttgcgacac agcggtttgt tgaaatggtc
     8041 gacattaatg aacgagcggt aagtctagcg caaggaaacg caaagcgtaa ccaaatcgaa
     8101 catgttgata tccatcaatc gaatatctat gaagcagttc atgaagaaac ctatgcagct
     8161 atcgtaagca atccgccgat ccgtgctgga aaaaaagttg tacatgaaat tttgacaggc
     8221 gcatatcctc ggttgaagaa gggcggtaca ctgacaattg tgatccagaa gaaacaagga
     8281 gcgccgagtg cgcaaaagaa aatggaagaa acatttggta atgcagaaat cgtcacaaaa
     8341 gataaaggat attatatcat caaaagtgtg aaagaataac tagctgaaaa atagtggagc
     8401 gacgaaagag tggaactggg cgtgcatgta cgggtacgta tcagttcttt ttttgttgag
     8461 aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga aatagtagga acagagtcgt
     8521 tgttttttac gataaaataa ataagataca gaacttgtat agaaggctgc caagtcggta
     8581 gataaaggag gtttttatca aaaaacatac tttccttttt tctctattct tttcttgggg
     8641 gttttggtac actgataggg aatttagata gaagggagcc aataggatga agctgaccat
     8701 cagagatatc gcagaaatgg caggcgtatc agtaacaact gtctcacaga ttttgaataa
     8761 caaaggaagc agatttagtg ataagacaag aaaaaaagtg ttgtcgatcg tgaatgaaca
     8821 tcattataag cccgattatt tcgcttcaaa tgtgatcaat cgacattcaa agaccatcgg
     8881 aatgatcgtc cctgatgtca ctgatttctt cttctccaaa ctgatcgaag gtgtagagag
     8941 ctatttgaat cctttgggct atgtgatcat gttatgcaat tctcgtcata gccaagaaaa
     9001 tgagatcaag tatttacaag agctttccca ccgctcagtg gacggaatct tgctggccac
     9061 accaaatatt ttacctgaga aatatgcatt agacagcggt ttttatcaaa aaatgccaat
     9121 cattttaatc gatcgcggat tgaatcgtcg ggataacgga cggcttatcg taaaggaata
     9181 tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat ggccatacga agatcggcat
     9241 gctgaaggaa accactggat actatcaatt agaagaacgt ttcaacggat atcgtcatgc
     9301 gctgaaagat catggcctgc cttttaatgg gaaatttgtt gaacatggag agttgactgt
     9361 ccaaggtgga tatactgcat caaaaaaatt actcaaacat aaagatatca cggctatttt
     9421 ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg atcgatgaat tgggcaaaaa
     9481 aatcccagaa gatatctcag tcataggatt cgatggatta aagctgtcag aatacatgat
     9541 tccgcaattg acgacagtgc agcagccaag ttttgatatt ggattttatg ctgcacgttt
     9601 cctgatcgat acgatcgaat ttcctcaaag aaaagttccg aacaaagtgt tcgaaacaaa
     9661 attaattatt cgtgagagcg taaaagtatt gacaaagggc taactcggcg gtatattgct
     9721 tatgtaagca tttacaaagg ctagttagtg ctttttttat ttttagtaaa ccagtttact
     9781 aaagtggttt acctttccta aaaataatgt ataataccga atgaagacaa ggaggatcag
     9841 gcaatgcgaa tggtggatct gattgaaaaa aagagggatg gaaacgagct gtcgaaagag
     9901 gaaatcgagt acatcgtcac gaactatacg aatggaaaga ttccagatta tcaagtcagt
     9961 gcattattga tggcaatttt ttatcaagac atgacaaatg aagaaatcac gaacttaaca
    10021 ttggcgattg ctaattctgg tgatgtcatc gatttgagtt ctcttgaagg aataaaggta
    10081 gataagcatt cgacaggtgg cgttggtgac acgaccacat tgattttagc ccccctagta
    10141 gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg gtctaggtta cacaggtggt
    10201 acactagaca agctagaagc aattcccgga tttcagatcg agctttcgga tgaagaattt
    10261 gtacgcattg ttaatgaaag taaagtagcg gtaattggtc aatcaggaaa cttggcaccg
    10321 gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga ccgttgattc tcttccgctg
    10381 attgccagtt cgattatgag taaaaaaatc gcggcaggag cagatgcaat cgttttggat
    10441 gtgaccactg gagacggtgc tttcatgaag aacatcgaag atgcaagacg tctggcaaag
    10501 accatgacga gcattgggaa actggctaac cgagaaacag tggcggttat ctcagatatg
    10561 tcagaacctc ttggcgaagc tatcggaaat agtctagaag ttgtcgaggc aatcgaaaca
    10621 ttacaaggaa atggtccaga agatctagta gaaatgtgct acgccttagg aagccaaatg
    10681 gttgttcttg ctggcaaagc taagacaacc gatgaagcaa gaacgttgct tcaagaagct
    10741 ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga tccaaaatca agggggagac
    10801 ccagcgatcg tggaacatcc agaacgaatt ttgactgcac gctacacaat ggagcttcct
    10861 gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg aattaggaat tgctgcaatg
    10921 atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg atcacgctgt tggattgaaa
    10981 ctgcataaaa aaattggtga cactgtgaca aaaggtgagt cactattgac gatctatagt
    11041 aacgacgaag aaatcacttc tgtcattgaa ttattatata aaaatattga aattggtgaa
    11101 tcggcaatga agccaacgtt gattcatgac atcatcacag aataaaggaa aaaagcggac
    11161 tttaccgttt taaagaaaag gggaaaagaa atggaactaa accgaatgat cgaccatact
    11221 attttgaaag cagacgcatc taaagaagct gtgatgcaaa ttatcgaaga agcaaaaaaa
    11281 taccattttt attctgtctg catcaatcca gcatgggttg cgctagcgaa agaacaatta
    11341 caaggtgagc cagtagccgt atgtacagta atcgggtttc ctctaggagc aaacacaccc
    11401 gagacaaaag catttgaaac aacagatgcc atcaataacg gtgcagatga agtcgatatg
    11461 gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac aagtccaaaa agatatcgaa
    11521 gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag ttattattga aacatcgtta
    11581 ttagatagag aagaaatcat ccgagcttgc gaaatagcaa aagctgcagg cgctgatttt
    11641 gtcaaaacat caactggttt ctcaacaggc ggagcaaagg tagaggatgt ccgtttgatg
    11701 cgtgaaactg taggaccaga aatgggtgtc aaagcttccg gcggtatcca taacgaagaa
    11761 gaagcaatgg caatgatcga agctggtgct acaagaatcg gaacaagcgc aggagtagca
    11821 atcgtatctg gttcaactgg tgaaggctat taattttttc aaaggagaag ctcatggcag
    11881 aactcaaaca agaatggatc aacacagcta ttgaagcgtt agataaagcc tatgttcctt
    11941 attcccattt tccggtaggc gcttgtctcg taacggaatc aggcaagatt tatcaaggca
    12001 tcaacattga gaatgcatca tttggattaa cgaattgtgc cgagcgcaca gcttttttca
    12061 aagcagtatc tgaaggagaa aaaacattta cgcatctagt cgtagcgggg cacacccccg
    12121 atcctatctc tccttgtggt gcatgtcgac aagtaatggc agaattctgt tcccctgata
    12181 tgccggttac gctagtagga gacaatggtg tgacgaaggc aacgaccgtt cgtgcactgc
    12241 tgccttatgc atttactgaa aaagatctat aactcatgtt cattcagtca gctgactgtt
    12301 gaaaaataaa acaattattt taggaggctt tacagaaatg aaaaaagcaa aattatttgg
    12361 tttaggtgct gtcgcactag cagcagggtt atttttaggt gcatgcggca acaacggctc
    12421 tactgattca tccggtggca aaagctcatc agacacaaca actgcagcat tgatcacaga
    12481 cacaggtggt gtagacgacc gttcattcaa ccagtcagct tgggaaggac tagaaaaatg
    12541 ggggaaagat caaggacttt cacgaggaaa tgatggcttc caatatttcc aatcttcaaa
    12601 tgaatcagat tacatcccaa atattgacca agcattgaat gctggattca aaacgatttt
    12661 tggtattggt tataaactaa aaccagcgat ccaagaacaa gcaacaaaca atacaggtac
    12721 gaactttgtt attatcgatg atgtcatcga tggattagat aacgttgtat ctgcaacttt
    12781 caaagacaac gaagcttctt atcttgcagg tgttgcagca gcttatacga ctgaaacaaa
    12841 cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc gatcgttttg atgctggatt
    12901 caaagccggt gttgatgcag gtgcgaaaga attaggaaaa gaaatcaaag tattgaatca
    12961 atatgctggt gacttctctg ctccagataa aggacgttca attgctcaag gtatgtatgc
    13021 acaaaacgca gatatcatct tccatgcttc aggcggtaca ggtaacggtg tattccaaga
    13081 agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg gttatcggtg ttgaccgcga
    13141 ccaatcagac gaaggcgaat acacattaaa cggcgaaaag aaaaacttca cattgacatc
    13201 tacactgaaa gcagttggaa cagtagtaga agatttggca caaaaatcag cggacggtaa
    13261 attccctggc ggcgaacaca ctgtttatgg attgaaagaa gacggtgttg gtctaacaga
    13321 aggacaactt tctgacgaag cgaaaaaagc agttgatgaa gcaaaagaaa aaatcatttc
    13381 aggtgacgta aaagttccag aaactcctga agaaaactaa aaccatttct atagccttgt
    13441 tagacacatg tttgacaagg ctatggtttg attagaacaa gaattgttca tttatccaag
    13501 ctgccttgtg acagtttgga taagggaaca actccctgaa gtaatgtttg tttcaaaaga
    13561 agaacgctca ctaagcgtga atcttagtca atcgttgccc tagatagaaa tcgttaggat
    13621 gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat gcagaatatt accaaagaat
    13681 ttggaacatt caaggcaaat gacaacatca atttacaagt aaaggcagga gaaattcatg
    13741 cgttgcttgg agaaaacggt gctggcaaat ctacattgat gaacgtgctt tccggattat
    13801 tagagccgac atcagggaaa attttgatgc gtgggaaaga agtaaagatc acaagtccga
    13861 ccaaagccaa ccaattaggg attgggatgg tccatcagca ctttatgctt gttgatgcct
    13921 ttactgtaac agaaaacatc gtgttgggaa gcgagcctag tcgcgcaggg atgcttgacc
    13981 ataaaaaagc acgaaaagag atccaaaaag tttctgaaca gtatggattg tcagtcaacc
    14041 cagatgctta cgttcgtgat atttcagttg gaatggaaca acgggtagaa attttaaaaa
    14101 cactttaccg aggagcagat gtactgattt ttgatgagcc gacagctgta ttgacccctc
    14161 aagaaattga tgaattaatc gtgatcatga aggaattagt caaagaaggc aagtcgatca
    14221 ttttgattac gcataagtta gatgaaatca aagcagtagc tgaccgctgt acagttatcc
    14281 gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt aacctcacaa caattagccg
    14341 atatgatggt tggaagaacg gtttcattca aaacgatgaa aaaagaagcg aagcctcaag
    14401 aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa tcgtggatta gaagccgtga
    14461 aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg tatcgctgga atcgatggaa
    14521 acgggcagtc ggagttgatc caagctttga ctggtttgcg aaaggcagaa agcggacata
    14581 tcaagctaaa aggggaagac atcactaata aaaaaccccg aaaaatcact gaacatggtg
    14641 taggacacgt gccagaagac cgtcataaat acgggttagt cctagatatg acattatctg
    14701 aaaatatcgc actacaaacc tatcatcaaa aaccttacag taaaaacggc atgctgaatt
    14761 attcagtaat gaatgaacat gccagagaat tgatcgaaga atatgatgtt cgaacaacga
    14821 atgaacttgt tcccgcaaaa gctttatcag gcggaaatca gcaaaaagca attatcgctc
    14881 gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa tccaactcgt ggactggatg
    14941 taggtgcgat cgaatttatt cataaacgtc tgatcgaaca aagggacaaa tacaaagcag
    15001 tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt ttcggatcgt attgctgtta
    15061 tccatgaagg agaaattgtc ggtatcgttg atccgaaaga aacatctgaa aatgaattag
    15121 gtctattgat ggcgggctat accttagaag aagcacgaaa agagttagaa aagggggctg
    15181 gcgcacatga atgatcgcag cgaaaaattc cgtaatttat tagtgcctgt actttcagtt
    15241 gttttaggat tgatcttagg cgcgatattg atggctgctt ttggttttca gcccatccaa
    15301 ggttattctt ctatgctaga tgcagccctt ggttcgcaaa gagggatagg tgaaacgtta
    15361 aggcaagcaa cgccgttgat ttttacagca ttaggttttt ctgtagctaa ctcagctggg
    15421 tttttcaata ttgggctttc tggacaagca ctttgcgggt ggatcgtcag tgtctgggtg
    15481 gcacttgcgt ttccagatct gccaaaagtt gttttgcttc cgttatgcgt gatcttaggt
    15541 gccttagctg gtgcgatggc agcggctgtt ccaggtttac ttcgagcatt ttttggaaca
    15601 agtgaagtga tcgtaacaat catgatgaac tatatcttat tatacgtaag cacatttgct
    15661 ttacatgaag tgatgcctga aagctatcgc tcaagtttgg actcttccaa tatgatcagt
    15721 caaaatgcct cactgcggtt accatggtta acacaagctt ttggcggctc tcgcgtaaac
    15781 tcaggattat ttttagcaat catcgcgctt attttcgttt ggctgctgat gaagaaaacg
    15841 actttaggat ttgaaatccg ttctgttgga ttgaatcctt ttgcttctga gtatgcagga
    15901 atgagtagta aacgtacgat cgttttgtcg atggtcattt ctggcgcttt ggcagggctt
    15961 ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct ttgttcaaac gacttcgcta
    16021 tctattggat ttgacggaat ggctgtttcc ttgcttggtt ctggatcagc aatcggtatt
    16081 cttttatcag cgatcttgtt cagtattttg aagatcggtg gacttggcat gcagacagga
    16141 gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt tgattatctt ctttgtcggg
    16201 atcaacttcc tgattcgttt cgtcatggct aaagtcttcc aagggaaaaa acaagaagag
    16261 atcgttgcga caatagaatc gaaaccaacg aatcaaaacc atgaaggggg cgaactataa
    16321 tgtcgactat cgaattaatt gccttgatcc ttacttctac actggtttat tcaacaccgc
    16381 taattctaac gtcactaggc ggaacttttt ctgaacgaag cgggatcgta aacgtagggc
    16441 tagagggaat catggtcatg ggtgctttta gttccatcgt tttcaacttg acgatgagtc
    16501 catcacttgg ttctatgac
//
LOCUS       DAPBAB010000246        12145 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.068, whole genome shotgun sequence.
ACCESSION   DAPBAB010000246 DAPBAB010000000
VERSION     DAPBAB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.068"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..835
                     /locus_tag="RD444_003166"
     CDS             <1..835
                     /locus_tag="RD444_003166"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7550421.1"
                     /translation="DLLDKPTDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCS
                     DGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQG
                     AAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLG
                     AKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPII
                     AICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILP
                     K"
     gene            1093..2595
                     /gene="eat(A)"
                     /locus_tag="RD444_003167"
     CDS             1093..2595
                     /gene="eat(A)"
                     /locus_tag="RD444_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDT7550422.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2662..3189
                     /locus_tag="RD444_003168"
     CDS             2662..3189
                     /locus_tag="RD444_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7550423.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3400..4461
                     /locus_tag="RD444_003169"
     CDS             3400..4461
                     /locus_tag="RD444_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7550424.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4464..5360
                     /gene="murQ"
                     /locus_tag="RD444_003170"
     CDS             4464..5360
                     /gene="murQ"
                     /locus_tag="RD444_003170"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDT7550425.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5374..7356
                     /locus_tag="RD444_003171"
     CDS             5374..7356
                     /locus_tag="RD444_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7550426.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7421..7867)
                     /gene="nrdI"
                     /locus_tag="RD444_003172"
     CDS             complement(7421..7867)
                     /gene="nrdI"
                     /locus_tag="RD444_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDT7550427.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8078..9502
                     /locus_tag="RD444_003173"
     CDS             8078..9502
                     /locus_tag="RD444_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7550428.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(9683..10456)
                     /locus_tag="RD444_003174"
     CDS             complement(9683..10456)
                     /locus_tag="RD444_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7550429.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10453..11358)
                     /locus_tag="RD444_003175"
     CDS             complement(10453..11358)
                     /locus_tag="RD444_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7550430.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11512..11832)
                     /locus_tag="RD444_003176"
     CDS             complement(11512..11832)
                     /locus_tag="RD444_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDT7550431.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         3903 a         2157 c         2607 g         3478 t
ORIGIN      
        1 agatctactt gataaaccaa cggatcaaac tatccgactt gcttccagtt atgccttagg
       61 agaacttatc aaagatgcac tgcttcaaga agtagaacaa attattgtca ctttaggagg
      121 aagcgggtgt agcgatggtg gtctaggatt actccaatcc ttgggtgctt ctttagaagg
      181 gtacacagaa ggaaatcctt tattatctac taaaaaactt cattttgatc gaagaaacgt
      241 tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt accaatccat atacaggtca
      301 acaaggagct gcttggcaat ttggaagaca aaaaggagga atagatacga cgctagcttt
      361 cttagataaa caagcaggaa agattgctca gcaaataaaa gatttctggg aggtggattt
      421 agatgatatt ccaggaagtg gagcagcagg cggtattggt ggagcccttt ttttgttggg
      481 agcgaagatg gtttctggtt tcgatttagt cagccaaatc actggattgg aaaaaaagat
      541 cgaacaagca gatgtcatca ttacaggaga agggcggatc gatcagcaaa cgatccacgg
      601 aaaagttcca cacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat
      661 ttgcggaagt agagaccaag agctagaagg actctctgat tttctcccaa ttgtcttaag
      721 tattcatttg ggtccaatag gtttagaaga agcaatggaa cacaggcaaa cattagaaaa
      781 aacaaaaatc ttaggacaga cacttagcag atttttcacc atcctcccga agtagttgac
      841 aaaatcattt tctttatgct atcgtaattt caaatatagg acaaagtgag ggatccgtgc
      901 attgaaaaat taagtcgatt gatgaaagct gcattcagac tgtaccttgt ggagacaagg
      961 aagcagtgtg cagattttcg ggattggctt ggttttttcg atgtatgggt ctttttgtgt
     1021 gtatccaacc cttgcatcct ctttagccag ttctgaaaat aggactggtc tttttttacg
     1081 aggtgatgga aaatgtctaa aatcgaaata aaaaatctga cattcggcta cgacagccaa
     1141 ggcacattat tatttgaaca agcaaatcta aattttgaca cacaatggaa actaggactt
     1201 atcggacgaa acggtcgagg aaagacaact ttactgaata ttctacaaaa caaactacct
     1261 tatcaagggc aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat
     1321 aaagaacgtt taacctatta cgtgttaaat gatattacgg attttgagat atgggaaatc
     1381 gaaagagagc tccaattgat gcaaacagat cctgaaatct tatggagaga attcagcaca
     1441 ctatcggggg gagagaagac aaaagtccta ctggcacttt tatttgtgga tgacactcat
     1501 ttcccgttaa tcgatgaacc aacgaatcat ttggatatct ctggtagaaa acaagtagcg
     1561 gcttatttga aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg gggatttatc
     1621 gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc aactggaact ttatcaaggg
     1681 aatttctcta tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat
     1741 gaaaaattga aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa agccgaatgg
     1801 tctcgttccc gagaaggaga taaaacaaag aaacaagtcg gattcatcga tactgaatct
     1861 agacgagtga ataaaggagc agtgggtgct gatgctgcac ggacgatgaa acgatccaaa
     1921 gcaatcgtga atcggatgga gacccagatc agcgagaaag aaaaactatt aaaagatatc
     1981 gaatatatcg attctttgac gatgaatagc caagcgtctc accataagcg acttttaagc
     2041 gtagaagatc ttcaattagg gtatgaaaat ctgttattcg agccaattca ttttacaatc
     2101 gagcctcatc agcgggtggc gatttcaggt cctaacggtg caggaaagtc atccattatc
     2161 cattatcttc tgggggcatt caacggcaag gttataggag aaaaatccca gccaaaacat
     2221 ctgagcatta gttatgtacg ccaaaattat gaagacaatc gaggaacgtt ggcggaattt
     2281 gcagagaaaa accaagtaga ctaccaagca tttttgaaca acctccgaaa gcttgggatg
     2341 gaaagagatg tttttcataa caagatcgag cagatgagta tgggccaacg gaaaaaagtg
     2401 gaattggcta aatctttatc acagccagct gaactatata catgggatga accattgaat
     2461 tatttggatg tcttcaatca agaacaatta gaacaactga tcttgaacgt gaaacctgcc
     2521 atgttactag tggaacatga tcaaaccttc ctggataaag tatctactga gattatttct
     2581 cttgagagaa tctaaccctt gtaatctcaa gaacaacaag gtatggttaa ggggaaattt
     2641 gccaacgaag gagagaatca catgctttat gtaacgagac acggagaaac aacttggaac
     2701 gcacaaggat tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg acagatgcag
     2761 gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa aatcatccat
     2821 tcccctcttc aacgagcaag agatacagca caagcagttg ctgatcgtct cagcttgccg
     2881 ctgaccgttg atgaacgatt ggtagaaatg gattttgggg attatgatgg actgccaagc
     2941 aaagatgaga actttcaaaa agcacgttta gcgttcgctg tacgattccc aaatggggag
     3001 tcagtcttag atgtgtatgc acggatcgtt cctttattga aagaatgcat cgaagacgaa
     3061 gaaaatgtct atctgttagt ttgtcataat gccttgataa gagtgatcaa tgcttatttt
     3121 catccgatgc ctaatgaggg gttctttaca ttcatggtag ataatacaga attggtttct
     3181 tatgagtaat tatgtttttg tgttatttaa ataaaatatt tcaaggcact gcttttgctt
     3241 agcagtgcct tttttaggtt atgacaattc ttttgataat ataaactcaa cctttagaaa
     3301 tgaaatttaa ttattttaat aaaagtattg aaattttgtt tctttagata tatactctag
     3361 tcatagaaat gaagacggat acagaaaaag agggataata tgtttggctt ttcaatattt
     3421 atgaacgatt ctttgtcaga agagaagaaa cagtatatcc gccaaatggc atccaatggc
     3481 tttgtcggta ttttcacttc catgcatata ccagaagatg atgtttcggc atatcaaaag
     3541 cgattgatcg aactaggaag cgaagcgaaa aagaataaac tagaattgat ggtcgatatt
     3601 tcaggagagg cattggaacg agcgggtttt tcattagaag atctaacgcc tttgaaagca
     3661 attggtgtaa caggtttaag aatggattat catatttcca atcagcagat tgccgactgg
     3721 tcacatcaat tgaaaatcag tttgaatgct agcacgatca cgcctaaaga tatcgatgaa
     3781 ttgaaagaag cagaagctga tttctcacag attgaagctt ggcataatta ttatccacgt
     3841 cccgaaacag ggctggacaa agaatggtat cagaaaaaaa atcaatggct caaaattcaa
     3901 ggtctccttg tccaaggttt tgtaccagga gatacagagt tgagaggccc actatatcaa
     3961 ggattgccaa ctttggaaga acatcgaggg atccatcctc ttgcaggcgc tctagatcta
     4021 ttagcaagta acacggatat tgtttacatc ggagatgcag gtctgtcaga aaatgttcag
     4081 gagcaatttg cttctttcca aaaagaacag acagtcttgc tccatacgga acctgtagat
     4141 gaagaatttt acgaatatat attagggaag catacgaatc ggcaagacga tgcaagagat
     4201 gtcatacgaa gcgcagatgc acgttttcga gagattccgc ctatcccagc aagaaataca
     4261 gctacgcgta tgaaagggtc tattacttta gataacgaaa agtatttgcg ctatatgggt
     4321 gagatccaat taaccaaata tgatttgcca gcggatgaaa aagtgaatgt agtagctaaa
     4381 gttatcaaag aagagctacc attgatcaac caaatcaaag ctggtatgaa ctatcaattt
     4441 ataagaaaag aaggaagata aaaatgagca aaatcaattt agagaaccta acaacggaac
     4501 gcagaaacca agaaactttt ggattagatg agatgagtat ttctcaagcc gttcaactaa
     4561 tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa agaactgaca agtatcgaac
     4621 cagtgattgc taacacgatt gagtccttta aaaaaggtgg acgtctgatt tatatgggag
     4681 ccggaacaag tggaagacta ggtgtgttag atgctgctga atgcgtaccg acattcggtg
     4741 tagaaccgga aatggtcata ggtctaattg ctggtggaga agaagcaatg actgtagctg
     4801 tcgaaggggc cgaagattct tatgagctag ggcaagaaga tctaatcaag ctgaatttga
     4861 caaaaaatga tatagttatt ggtatcgcag ctagcggtcg tacaccatat gtgatcggtg
     4921 gattagatta cgcaaaatcg atcggtgctg tcacaggaac gatttcttgc aataaacaag
     4981 ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga tgcaggtcca gaatttttga
     5041 caggctctac tcgattgaag tcaggaacag cacaaaaact tatcttgaat atgatttcta
     5101 ctatcagtat gattggtata ggtaaagtat tcaataactt gatggtcgat gtcaaaccaa
     5161 caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat gcaagcgaca gaagctgatt
     5221 acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa tgttaagctg gctatcgtca
     5281 tgattttgac aaatagtgat aaagaggaag ctcaagcaaa attagctgaa gcaaacggat
     5341 ttgtaaaagg gaccttatga gggggaaaag accatggcaa aaaacgaact gactttggaa
     5401 gaactggcaa aacaaattta taaaggggca ggcggcatgg gcaatgtaga aagcattgtc
     5461 cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg atcttgtaaa tgtctctgaa
     5521 ttaaaagcga ttccaggagt gcttggtgta gtagaagatg aacagcttca agtcatcatc
     5581 ggacctggga aagtgaacaa agtagcacag caaatggttg atttggcagg cgtgaaatta
     5641 ggagaaaaac tttcttctgt aagtgcagct acagcaaatt catcagcatc tggaaaagac
     5701 cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa aatcgaaaca aaaaccatct
     5761 aaaatcaaat cggtattaaa agacatttct aatatctttg tgccaatgat tcctgcattt
     5821 gtcggtactg ggatcgtggc agggattgcc gcagttttag caaacttagt cacagccgga
     5881 acacttgatg cagcaacatg gcaacaatat atcgatgtga tgaacatctt gaaaaacggg
     5941 atgtttagct acctagttat ctatacaggt atcaatgctg cacaagtttt tggtgcaaca
     6001 ccaactcttg gtggagttat cggggccgtt gtgatgctga cagggatgaa tccggaagca
     6061 ccactgaaaa acttatttac tggtgaagct cttgcagctg gacaaggtgg gatcctaggt
     6121 gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa aactgcataa agtagtacct
     6181 gatgcggtag atatcatcgt tacaccaact ctttcattga ttgccatcgg tctgattgaa
     6241 attttcttca tcatgccttt agcaggtttc atttctgatg gcatggtcgg tgggatcaac
     6301 tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc taggcacatt attcttgccg
     6361 atggttatgt tcggactgca tcaaattttg actccaatcc atgtgcaaat gattgatgaa
     6421 actggtcgta cgctattgct tccaattctt gcgatggctg gtgcaggaca agtgggtgca
     6481 gcattggcat tatggattcg ctgcaaaaaa gataaagaat taacagaaat gatcaaagga
     6541 gctttaccag tcgggatcct aggaatcggc gaaccattga tttacggagt aacattacct
     6601 ctaggacgcc catttattac tgcttgtatc ggtggaggta tcggcggggc agtgatcgga
     6661 atgatcggaa atgtcggggc tatcgctatt ggaccttcag gagccgcttt gatcccattg
     6721 atctctgacg gtaaatggtt aggctacatc cttggattat tggcagctta tgctggtggc
     6781 ttcgttgcta cattcttctt cggtattcct aaagaccgtt tagaaaaaga agaattagcg
     6841 actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag tacctcaaac ttcagctgaa
     6901 agtgtgacta tcctctcatc agttgctgac ggaacagttg tttctttaga taaagcaagt
     6961 gatccagttt ttgcacaaaa gatgatgggt gaaggctact ttgtagaacc agaaaacgga
     7021 catatctatt ctccagttac tggggtagtc agctctgtct tcccaacgaa acacgctatt
     7081 gggatcacaa cgccaaatgg tttagaaatc ttgcttcata tgggaatcaa cacagttgac
     7141 ttaggcggta cgccatttga tgtgaaagta acagaagggc agcaagtgac acctgatacg
     7201 cttgtagcag aagctgactt agaagcgatc aaagcagcag gcaaagaaac atccatgatc
     7261 gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct tggaaaaaac aggtaaagca
     7321 aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg atagaaacaa tagaaacgaa
     7381 agaagaaact gaaatctatt tttcagcttc ttctttttta ttatttattg ttttctgcta
     7441 aaatctgata aattcgtgtc acatcagatg gtgttccccg taattcataa tctgccaaga
     7501 aaggaaagcc aaatgtttgt gcatattgtt tggctgttag acaatactga ttattgaagt
     7561 ttttatttcc gctaccgact acaccaagac aaagtttatg attgtcttca tgttccagat
     7621 attcacgcat cgtttcggtc aagatttctg tatcaccgtt atctaaacca tttccgccgt
     7681 ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa tcactatttt
     7741 catggatttc ttttaaagta acttcaggat tgacaggatc ttctgcatgc attttttcag
     7801 catattctgc tagatgtttg gcaaaggcac gcgtatttcc tgaaatcgat atatataaaa
     7861 tgttcatgga tcatcctcca attctttcct tttagtatag catctttttt aagttttttg
     7921 gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa aagccggtag
     7981 aattatattt ctattaaagt ttttattaat taagtaaaaa taaaaaaggt ttgttatact
     8041 ttttgataaa agcggtttta ttttggagga tgcttacatg gatacagagc aacagaaaaa
     8101 aggaattggt ttaattccac ttgctgcctt agtaattggt tcagcgattg gaggtggcgt
     8161 atttggtatt atgacggaca tctccggatc ggctggcggc ccagctattt tcagttgggt
     8221 actcgtagga acagcaatgt tgatgctctc cttgtcgatc aataatatca ataaaaaacg
     8281 tccagattta aaaggcggga tctttagtta cgcagaagaa ggttttggcc gtttcgctgg
     8341 ttttatcagc ggttggggtt actggctcac atgctggtta ggcaacgtag cttttgcaac
     8401 attgttgatg agcgcattag gatatttctt cccagtattt gaaggtgggc aaaatctggc
     8461 atcgatcgtg atgagtacag tctttttatg gggatatgca tggctagtga atcggggagt
     8521 cgaaaatgct agtttcgtca atgctatcgt cacattctgt aaattagtgc cattattttt
     8581 atttattgta gtagctatca gcacgttttc gatccaacaa ttcattgata acttttctag
     8641 cgacattata ttgaatgcga atggacagcc gatcccgttt tccagtcaag tattgagttg
     8701 catcatggtg atgctttggg tatttgttgg tattgaagga gcctctgtcg taggttcacg
     8761 agcaaaaaaa caatcagatg ttgggaaagc aacgattcta gggatattcg gcttgattat
     8821 tatttacgtc ttggtttcca tgcttccata tgggacaatg acgatgaatc agctgcaaac
     8881 gatcaaggcg cagcctgcta tgggctatgt gtttgaaatg atggttggta aatggggagc
     8941 agtcgtgatc aacggcggtt tgatcatttc tttagttggt gtttggctct catggacgat
     9001 cttaccgatt gaaacaatgg gaaacatggc tgaagacgga cttttgccta aagcatgggg
     9061 aaaactcaac aaaaatgggg caccaactta tgctatcgta ttgacgacgt tatgtacgaa
     9121 tgtcttttta ctttcattat tattcactga tagtgcttat aactttgctt acacacttgg
     9181 tacagcagct attttcttca cgtggctttt cttaggattg taccaaatga aactttctta
     9241 tcagcggaaa gaatgggtcc aatttttagt agggctttta gcaagtgcct ttcaagtatg
     9301 ggctatgcta gtcgtagcct ttaatgaagt aatgcttgtt cttgttcttt tcttgccagg
     9361 catttatttt tacctcaaag cacgtaagga gcaaaacgag cctgtacaac atatccactt
     9421 agataagatt ttacttatag gtgtgacgat ggtcggagtg attgcaatgg ttttatttgc
     9481 cactgggatg cttcacgtat agaagtataa aagaagtata aagagaggtt gtgagcctgt
     9541 cgtttattca aaaacatact aggaaaggag ctgtgacaca aatgttgtca cagctccttt
     9601 ttcagaataa acggtgttta agagacagcc aatcggcaat aaggctgaac gtctctttca
     9661 cagcctcttt tttgatgagt catcatacat tttttcgctt aaatacgaga tatcctaatg
     9721 ctaagaacaa aacgatgtaa gctaaattac cgaatagcat ttgattagta gaaagatgag
     9781 tcaattgctt catgatctct tcgtttccaa tttggttttg cagattcaac atactgatcg
     9841 gattccattt gatccactcc catttttgga tgagtgcaat aaggatacca gatatgattg
     9901 acgaagcaaa ataaaagacg attcctgctg agatggatgc accggaacta ttgataaagc
     9961 aagcaatcat caacactaaa cttaaaatca gccataaacc gatataacta cccaaagtat
    10021 aagtgaacaa gctttggatc agtgtttgac cagtagaaac cttctccgtg aaggaaatcg
    10081 aagggaaaag aatcagtttc atcaacacag tgacaataat agctaaaagg tataaataaa
    10141 cagaataaat aactaatgtg atccatttgc ttactaatat ttctcctcga gaatactttc
    10201 gataaaggag atttttcaat gtaccatatt gtgcttccat cgaaatgatc gtgcttgcag
    10261 ctgcaatcat gataaagaca atccaagatg tggcactaaa aagctgagca gtcatttcaa
    10321 ctggatcaat gatcgtcgtg tattttttgg ctagaagtgc tgttcctatg agcaaaacaa
    10381 ctaatagaac agataaaaca gcagttgact ttttcttgat caatttaaat aattcttgtt
    10441 ttacaagcaa tgtcattttt cttcttcccc ctttactgaa tccagaattt ccaatagaga
    10501 agattccaaa tcattcgtca gatgctggac gtctgtcaca tggatatttt gtgcagacaa
    10561 tagctggatc acggttccta ataattcttc gttttctttt aaaagcttga ttttatcagg
    10621 atcttccaaa gtatatccag cttctgtcaa gatcgttcga gcagctagat catttgttgt
    10681 agagataacg atgaaatctt gcgctgtttg gttcaattca gccattgtac attgtttgat
    10741 gatctttcct tgattgatca ggatcagctc atcgaccatt ttttcaagct cgctgagtaa
    10801 gtgactggag atcaagaaag aggtgccctg agtagccaag tcggtaatga tttttcgcac
    10861 atctcttgtt gcttgtggat ccaaaccgtt catcggctca tctaaaataa cgatagacgg
    10921 atggttcagt aaggcaagag caattcccaa tttttgtttc attcccaacg agtaggattt
    10981 cgcttttttg tgaatgtact tttccatctt gagctgattc acgatagtgt cgatagcaga
    11041 tacatcgttg gtttcagaga ataacttcag atgatcatag cctgttaaga atggatagat
    11101 acccgggtat tcgatcaatg ctcctacttg ttccaaccct ttatgactag agggagaaat
    11161 ctcattttca ccaatacaga ttgttccatc tgagtaatgg atcagtccta aaatagcttt
    11221 catgatcgtt gtttttccag cgccattagg acctactaat ccaacgattt tgccagggga
    11281 taagctaaaa ctgacatcgc tgagggcttg tttgtgacca aagttcttgg ataattcttg
    11341 gaccgaaaga cttttcaaaa atattcactc cttttctata gatagtacaa gcatacacct
    11401 gatgagtgga gaagtcgcta ggtcattgga cttatttctt attttttcat aaaaaaattg
    11461 aatattaaat aaaaaggctt attttttctg atcagtactt attccaagtg tctagtggcc
    11521 gctagtaatc ttgatgccaa tgatactgat gagtagcatc cccacaaaga accaagtgga
    11581 aagattcagc ttatcatgaa acaagaccac accaattatg accgaaccaa ctgcgccaat
    11641 ccctgtccag ataggataag agagactcat cggcaaactt ttgattgcct ttgacaaaaa
    11701 gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa taattgattt tagaaaaacc
    11761 atcactccat ttcatcatcg ttgaccagaa tacttccaaa attcctgcta tgattaattc
    11821 catccatgcc atacttacat tcctccttaa ctataaaaaa atacacaaaa agaggaaaat
    11881 catcccttaa aagacctaat ggaatgatca tcctctttat cctacccggt ggcagatcgt
    11941 atatcttttt ctgtagtata gcacacctat ccagatatcg gcaatctcat actttttagt
    12001 aaagtgtacc aagcactgac ggatatatcg ttagcggaaa agaagatgaa tgctttgcaa
    12061 tttcatagaa agaagttgtt atttattaat catttaaaaa aaacagtaac tagaccttat
    12121 ttctatatat tatcgggcaa taaat
//
LOCUS       DAPBAB010000247        11274 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.074, whole genome shotgun sequence.
ACCESSION   DAPBAB010000247 DAPBAB010000000
VERSION     DAPBAB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.074"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            144..1055
                     /locus_tag="RD444_003177"
     CDS             144..1055
                     /locus_tag="RD444_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDT7550432.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1208..1693
                     /gene="vanZ"
                     /locus_tag="RD444_003178"
     CDS             1208..1693
                     /gene="vanZ"
                     /locus_tag="RD444_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDT7550433.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1980..2309)
                     /locus_tag="RD444_003179"
     CDS             complement(1980..2309)
                     /locus_tag="RD444_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7550434.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2614..2943
                     /locus_tag="RD444_003180"
     CDS             2614..2943
                     /locus_tag="RD444_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7550435.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2933..3220
                     /locus_tag="RD444_003181"
     CDS             2933..3220
                     /locus_tag="RD444_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7550436.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3499..4062)
                     /locus_tag="RD444_003182"
     CDS             complement(3499..4062)
                     /locus_tag="RD444_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550437.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(4255..4827)
                     /locus_tag="RD444_003183"
     CDS             complement(4255..4827)
                     /locus_tag="RD444_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDT7550438.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVNEALKKYDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKYRHLW
                     SETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            5615..6190
                     /locus_tag="RD444_003184"
     CDS             5615..6190
                     /locus_tag="RD444_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550439.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            complement(6373..7125)
                     /gene="istB"
                     /locus_tag="RD444_003185"
     CDS             complement(6373..7125)
                     /gene="istB"
                     /locus_tag="RD444_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDT7550440.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWHEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(7125..8666)
                     /gene="istA"
                     /locus_tag="RD444_003186"
     CDS             complement(7125..8666)
                     /gene="istA"
                     /locus_tag="RD444_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDT7550441.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            9580..10044
                     /locus_tag="RD444_003187"
     CDS             9580..10044
                     /locus_tag="RD444_003187"
                     /inference="COORDINATES: protein motif:HMM:NF037267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5626 family protein"
                     /protein_id="HDT7550442.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            complement(10041..10565)
                     /locus_tag="RD444_003188"
     CDS             complement(10041..10565)
                     /locus_tag="RD444_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7550443.1"
                     /translation="MQNDIRGKIKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSP
                     DINNLTLLSSLYEISIDDLLKEKKEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLV
                     LLLISLIGCLISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNI
                     FHWGGSTNVEYIEN"
     gene            complement(10615..>11274)
                     /locus_tag="RD444_003189"
     CDS             complement(10615..>11274)
                     /locus_tag="RD444_003189"
                     /inference="COORDINATES: protein motif:HMM:NF015755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7550444.1"
                     /translation="LFDSRFKTLQQNRYVDIIGYDTYDKINHAYAFGGVAMTINTIQA
                     YLDIPIDFFVTLNMQGLEQIVDSIGGITLTPTATFTQDSYSFYQGEIINLDGKASLAY
                     SRNRHADDSGDYGRQARQREIIMAIIEKGTKVTTLATKIPSLIKSLQGNMTTNLSVDD
                     MWTIINKYSTALGNIEQVQLKGEGQLLDDGIYYEMINEESLEEVQNILQKQLDYHLNI
                     D"
BASE COUNT         3596 a         1880 c         1976 g         3822 t
ORIGIN      
        1 gatttcagta cagaccgaaa acccgaagag agtgccttct acggggcaaa acggcacaat
       61 ctcaaacgag attgtgccgt ttaaggggaa gattctagaa atatttcata cttccaacta
      121 tatagttaag gaggagactg aaaatgaaga agttgttttt tttattgtta ttgttattct
      181 taatatactt aggttatgac tacgttaatg aagcactgtt ttctcaggaa aaagtcgaat
      241 ttcaaaatta tgatcaaaat cccaaagaac atttagaaaa tagtgggact tctgaaaata
      301 cccaagagaa aacaattaca gaagaacagg tttatcaagg aaatctgcta ttaatcaata
      361 gtaaatatcc tgttcgccaa gaaagtgtga agtcagatat cgtgaattta tctaaacatg
      421 acgaattaat aaatggatac gggttgcttg atagtaatat ttatatgtca aaagaaatag
      481 cacaaaaatt ttcagagatg gtcaatgatg ctgtaaaggg tggcgttagt cattttatta
      541 ttaatagtgg ctatcgagac tttgatgagc aaagtgtgct ttaccaagaa atgggggctg
      601 agtatgcctt accagcaggt tatagtgagc ataattcagg tttatcacta gatgtaggat
      661 caagcttgac gaaaatggaa cgagcccctg aaggaaagtg gatagaagaa aatgcttgga
      721 aatacgggtt cattttacgt tatccagagg acaaaacaga gttaacagga attcaatatg
      781 aaccatggca tattcgctat gttggtttac cacatagtgc gattatgaaa gaaaagaatt
      841 tcgttctcga ggaatatatg gattacctaa aagaagaaaa aaccatttct gttagtgtaa
      901 atggggaaaa atatgagatc ttttattatc ctgttactaa aaataccacc attcatgtgc
      961 cgactaatct tcgttatgag atatcaggaa acaatataga cggtgtaatt gtgacagtgt
     1021 ttcccggatc aacacatact aattcaagga ggtaaggatg gcggaatgaa accaacgaaa
     1081 ttaatgaaca gcattattgt actagcactt ttggggtaac gttagctttt taatttaaaa
     1141 cccacgttaa ctaggacatt gctatactaa tgatacaact taaacaaaag aattagagga
     1201 aattatattg ggaaaaatat tatctagagg attgctagct ttatatttag tgacactaat
     1261 ctggttagtg ttattcaaat tacaatacaa tattttatca gtatttaatt atcatcaaag
     1321 aagtcttaac ttgactccat ttactgctac tgggaatttc agagagatga tagataatgt
     1381 tataatcttt attccatttg gcttgctttt gaatgtcaat tttaaagaaa tcggattttt
     1441 acctaagttt gcttttgtac tggttttaag tcttactttt gaaataattc aatttatctt
     1501 cgctattgga gcgacagaca taacagatgt aattacaaat actgttggag gctttcttgg
     1561 actgaaatta tatggtttaa gcaataagca tatgaatcaa aaaaaattag acagagttat
     1621 tatttttgta ggtatacttt tgctcgtatt attgctcgtt taccgtaccc atttaagaat
     1681 aaattacgtg taagatgtct aaatcaagca atctgatctt tcatacacat aaagatattg
     1741 aatgaattgg attagatgga aaacgggatg tggggaaact cgcccgtagg tgtgaagtga
     1801 ggggaaaacc ggtgataaag taaaaagctt acctaacact atagtaacaa agaaagccca
     1861 attatcaatt ttagtgctga ggaattggtc tctttaataa atttccttaa cgttgtaaat
     1921 ccgcattttc ctgacggtac ccccaaacta agatttcata gatagatttt ctgataactt
     1981 taaaaaacac gaatagcgga aaggatgtct tcattcgcta aagagtaata aactactttt
     2041 ccatcttgac gtgattttgc cattccatgt ttttttaaaa aacgtaaatg gtgtgacgtt
     2101 gtagcgacac tagcagtaag tatttctgct aaatcacaga cgcacatttc cttataggtt
     2161 tctaaagcat agaaaatttt aattctagaa aaatcgctga aacatttccc taacacaagt
     2221 aagtttgaaa aatctttttg atccaattta ttttttattc catttacttt ttctttatgt
     2281 acgacagtca cttggcaaac ttttttcatt catctcacct cacattcaaa cgattatttg
     2341 aatgaagtat acctatttga tcactctttt gtcaatctac cttaattcca atacactatt
     2401 ataagcaatt tcatgtattg aagtaaactt acttgcaagt gcgtttttga ttccaagtta
     2461 aaaacatatt attattctac ataatttcct aatcaaataa aactcctttg aaaattgttc
     2521 taaaggagtt ttattatttt gtatttctac taaaaatagc gtacaatgta attactaagt
     2581 aattacgaaa tgtaaaataa tggaggaaat aatatgatta caaaaacaag aaaacaagga
     2641 aattctatta tgttaacggt ccctaaagaa ttcgatgtgc ccaatggtgt tgaagtagaa
     2701 gctaagcttg tagagaatgg tatcctttat gagtttgtag agcctaaaaa agaatttttt
     2761 gattttagtg aagatatttt ggctgatatt ctttcagaag gttatagcaa acaagagatc
     2821 ttaaaagaat ttagaaatag aaaaaatgcg ttaacttctg catttaaatc tattgctgaa
     2881 gatactgttg caaattctaa accgatgaca aaagaggagt tggcagcaga aattggctta
     2941 tgagattcta ccttctaaac acgtaataaa gtatctaaaa aaattaaaag agaaaacatt
     3001 aaaagaacaa tttttaacga taatttatga tgagatagca gttcgtcctc attcgggaga
     3061 acaaaaaacg ggtgatttat caggaatttg ggctattgga ttcaagtatg caggtacgac
     3121 atatcgcgtt gcatatgaaa ttaaagataa tgcagtgata cctatcttac tatgtggaac
     3181 gcatgaaaat ttttatgaac aattaaaaaa gattaggtaa gaacccaatg tttcaacttg
     3241 agaggataga cgacacttta aaagataatt tttaacagct gagagttgta ataccctata
     3301 attttaaacc actaacctat atatttatga aaaatagaaa aaattaaaac gcattttaag
     3361 cctctttcaa attaaaagct tcatccttat gcacaaagag ggcttaaaat gcgttatttt
     3421 atattgtttt ttagtaataa taaaaaaatc actctaattc tcccataaat attatagatt
     3481 cctaaagatt aaaatagatt attccctatt gtcttttaag tagcggtaga gtgttgcctt
     3541 gctaatacca gtcatttccg taatttcttt tacgctataa acctgacttt tatacaattt
     3601 tactgctcga tcaacatctt tctggttcac tcgtggacgg ccacctttac gacctctcgc
     3661 acgtgcactt ttcaaacctt ctttggtacg ttcaacaatt aaatcacgtt cgaactgact
     3721 aaatgcttgg aagacagtca tcattaagcg tccgtgaggc gtgctggtat cgaagttttc
     3781 tttcaagctc accagcttaa catggtgttc ttcaaaatag gtaacaaggt caatcaaatc
     3841 ctttgtacta cgacccaatc gagaaaaact ttctacaatt accgtatctc ccggacgaat
     3901 tttatctttt aaacgattta gttggggacg aaccgctttg gttcccgtca ttttctcagt
     3961 taggatttca ttacaatttt gttcattgag taagtctaat tgacgattta gttcttgtga
     4021 acgtgtactc actcgggcat aaccataaat gtatccactc atgcttttct tcctttcgta
     4081 ctttaattct ggcttgattg tatcataaaa gggagttata gatacatagt taatgcaaca
     4141 agttatgata caaaatatga cctcttttat ttaataaaag aggtcatttc attttgtctc
     4201 ataatcgatt gtttttgata cggtttttag taataaatgg agactcttct ggtatcatga
     4261 atcagaagag tctccatctg catacatatg tattttattt atagcatcta aactgttttc
     4321 agcaataaaa gtaggttttg tttcactcca cagatgtcta tatttggtta aactatcaat
     4381 tccctctccc gttaaaagta aaacactgtt tataccagca tttttggcca acatcatatc
     4441 gtttttaccg gaatcgccta ctacgaaaca cttacttgga tttaaatcgt atttttttaa
     4501 tgcttcattg acaaatgtag gttgaggctt cttgcaatca caattgtctt ttctagcatg
     4561 agggcaagtg tatacatccg ttatttgaat gtcatttttc tctaactcat ttattaagct
     4621 ctctaaggca tcagtatatt ctgccatagt aatacgtcca tgtgaaatat gactttgatt
     4681 cgtaatgatg atatttagat aatgactttg tgccgctttt tttaaggccg gtattgtgtc
     4741 tttaaaaaaa ttgaaatttt gattaatcag tgttctacta tcccctccca gcgtacctaa
     4801 cagatcccaa aatatagctt tattcatgga tatttgtctc cctctcattt taagaatagt
     4861 taactaacaa gccaaggtgc ttacagtatt taaaagtagt tcttcgtaat taaaacaaaa
     4921 atattacttt ttgaaaaagc atattttatt ttgccagttt aactatatca tatttacagg
     4981 agccactctc tattcacttt ctatatgaac ttcttttgag ggaatggata tttcgaatca
     5041 cataatcact gatatagaag agacttagaa tttcagttat attttgatac agcaaagagt
     5101 gtaaaaaaga agataccctc gttggcgaaa aacatttgaa tacaaacctt acttttttga
     5161 tgggatttcc atgctaggag cacataaaaa actaaggtga taataaattt atagtgattt
     5221 agtgtggagg ttcttttgtt aattgaaacc ataaatagta aaaaaatcta tttttaatga
     5281 ttgtacatat taaagagaaa attgatatac accagcttat caaagttgag attaatacta
     5341 ctactttgac ctatgaagat gattttataa ataccttata gtaaagaaaa gttcaatttt
     5401 ttctgctatc atcttttttt ataaaagcaa cgattagttt cccagatgta aatagaaaag
     5461 tgcttttgaa acagctcgaa aaacataata atagattgtg agaggaggaa aaactattgc
     5521 aaaatcatta cgtaagagat gttactcttg cttagatttt ttatttttca agttgtggta
     5581 ttaaaagtag aaaaaataga aagaaggata aaaaatgaaa aataaattta agtatttatt
     5641 tactatttta gccttattgc caattactac atcgacctta attactccta caatagccaa
     5701 agcttctgaa aaacaggaag attctgttag ttatgaaatt gtaaaaaatg atctttacat
     5761 tcagtttgaa gaagagttag ctgaattgat tgcttccaat caatcaactg tttcagtccc
     5821 tgatttattt tctacacaat cagtattatc tacttctagt caagataatt ctattagtga
     5881 agagcaattt ttttctttag tcgaaaaata tgatggagct gaattgacag ctgttgttaa
     5941 ttcacaagat gctttcacaa ctaaatttgt gaatggtggg gttaactcat atgctacttc
     6001 ttacagagga ttaaatgcat tgcgtagcga gttgaagtat aatgctggtg ctttgaaagt
     6061 aggaggtacc ttagttggca cagctggggg attgttagga gtattattag gcatggttac
     6121 aggggattat ttatctggcc attgcgatga tgcagcagca ggtgttagtg cctttattca
     6181 agctggttga atacggcatc gaaaatgtac cactagagca ttgtttttta ccactaagtg
     6241 caaagaaatg taccagtaag tgcgacgcgg ttaccatttc aaataataaa agcgacaata
     6301 atgaccacaa ggtcaactaa gagccactca tgaaaatcat ggagtggctc ttaatttatt
     6361 ttaatgactc atttaattac ggatgccgta acgttcacgc atagaaactt taccgtctac
     6421 cagaatttga taagaatcat gtatgatcct atctagaatg gcatcagcga tttgggggtt
     6481 gccgagcttt tcgtgccatc cttctggatg gaattgagaa cagaaaatag ttgatgctgt
     6541 tttaagacgg gattcgatta tttccaatag aaaggtggaa ttttctaacg aaagattggt
     6601 taatagccat tcatcgatga tgagtaattg aactttttta tatctgttga ttatttttcg
     6661 aaaactacca tctgcttgat ttttcgagat ggttaattca tctagtagtt cagggagtcg
     6721 gatgtatttc acgttgtagt attgccggca tgcctgtatt ccgaaggcat tggcgatata
     6781 cgttttccca ttccctgaag cgcccatcaa aatgatgtta tggtgttctt ccaaataagt
     6841 cccactggct agttttaaaa tgaggttctt atctaaatta cggtccggat ggtattcgat
     6901 atcctcaatg ctggcagagg catttctaaa gtttgcagat ttaatcaacc gctgtagttt
     6961 attatttttt cgccgggaat gctccagatc aaccatgagg ttgaagcggt cttcgaaact
     7021 catggattgg tactctttat ttgtcatctg ttcacgatag gattcaagca tgcctgaaag
     7081 tttcaattca gctaatttta cttctgtttg ctcatttaac atcattattt ccctccaaaa
     7141 taggacgcac cgcgagtgaa tccatgcttt tcaatgttag tgtcttcaat atgtgattgg
     7201 cttgttgctc gcttctgctt gttgcccttt agaatggtct ggataatttt aacagaaggc
     7261 aaagaagcca gatttaaagc tgttgaacag gccgtttcta tttcattttt agaatacttt
     7321 ttaccaaagc tcttcaaaga catgatcgac tttaaggctt gtttttcatt ttgataacgg
     7381 tctaagaggt gttgaacaac ctttactgtt gaggaaccga tggtttcagc ccattccatc
     7441 gaggattctg gggtatggtc cacatacatt ttgtgattat ctggcatatg atctctgaca
     7501 gtcgaatact gcccaaattt cccgtacaga cgtttatgtg ttgcgattcg catggaatta
     7561 aaatatagtt caattagatt ctctgtcatt cgaatatcta tattttgttg aatgtattcg
     7621 tatggaacgg aataaaacat gctttcaact gagacgtggt agtttagttg aacctttgct
     7681 tttttccaga cagacatctt ataaggtgcc agtggaagag gattaagtgc gaatttttct
     7741 tcttcctcga aggctctgat cctggatcct ggtttcttag tgaacggtcg ctcattgaat
     7801 tcatttaatt tcattctgat ttcacgattt aaatcttcga ttgtaaaaca atgcgtattg
     7861 cgtaaggcag ctataatcca agtggagatg acgcctacgc tgccttcaac actgggctta
     7921 tccttgggag tagaaactcg ggcaggtaaa atgatggacc cgtaatgttc ccctagctct
     7981 cgatatgatt cattaaggat gacatcgatg ccttcattct tttttacacc tgtttttaga
     8041 ttatcgggaa caattatgtc ggtagaaccg ccaaaatatt caaatgcatg gttatgtgcc
     8101 gtaatccaag ccaccgattt catcgttagg aatgcttccg cataagacaa ttggctgcac
     8161 ggtaaggttg ccacaaagac ataagccttt actttttcac ccgtatctcg atcgatgaca
     8221 taagctggcg atccagccca atccacttcc ataatttcac caggcttccg acggatgcgc
     8281 atagtggctt tatgctttcg cgcgaacgca ttgtaatagt cacaaaatgt acgataggcg
     8341 taagggattt tcccactcat gcgacactca actgcgtatt cctgatgaag aagcgtcaag
     8401 gtcacgtttg gtttggccaa ttccttgtga atcttgtcga agtcaggcag gtgccgtcca
     8461 ttggcttcta attgtttttc tggaaacaaa aattcgccta gccattgatc ggtcatttct
     8521 tccgacaacg ccctcgctat ccctttctcc tcagcttttc ggataacctc agtaattttc
     8581 tgacgtgagt tccccgtgct tgctgcaata ctgcgaatac taattccttc actgtgcatt
     8641 tcaagtatcc ttcgataacg aaacataata aaaatacctc ctgtaaaata aatgtcacac
     8701 cctaaggtgt gcacctacat tatacaagag gtatttgtgg tacactgctt tgcacttact
     8761 ggtacatttc tttgccgagc ctggtaaaaa ttattgcaga gatggtacat tttcgatgcc
     8821 gtaatcactg gttacactag aggaggatct agggtaaccc ttactgaagg tttccctatt
     8881 agccatattg atagtattta tgaagctgta attaattaag caagaaatgt ggagatgata
     8941 aaaagcagtt ctctcttggg taaagagaaa actgctttta tctttttcgc taatgctttt
     9001 tggaattacc tcgctgtcta aaaagggaag tcacagaaaa aagaattaac acactaccta
     9061 ttattattgg tagtacagtt aaaagagtaa tcttactaag atcaaaatca gaacttgcta
     9121 cactattaat aacaaaaaac aagccaacca aaaaagtaaa tacagataga tatttattag
     9181 tcataaaaga atcttcctct cgtatttttt tgttctagga agctatgcta atctaagttc
     9241 ttcctaagta tagtgtctca tctaagtata gcttaattaa gcatcttatg gtttacatca
     9301 ggtcacttta tagttgcaac cgctcaaatt tgctttttga ttttattatc tacgttatta
     9361 gtaaaaaatt agttgatttg gtttttttat ttcgttgata ttttatctgt ttagtaagta
     9421 gagttggtaa cagtgaatgt ttactaaccc agatacactt atatatgcgt ccatttgtag
     9481 agagagccgt ttcaatataa gaatgttcac aaaatgttct cttactaatt aaacccgtat
     9541 atattaaact atgagtatat taattttagg agggattttt tgagacaagt tttattggtt
     9601 gttactttat gtttatcttt ctttttattt gaagtaaaca ctgtagaagc atatgtggca
     9661 aataatggag tctactataa cttaatccaa ggtggcacgc aagagttcat ggaggtagac
     9721 gaagaaggaa atcctatctt gattcgtgtg gaagaggttc ccgggctctc cctgttctcc
     9781 gtcaacaacg gaaattataa aatatctgga tctaaatttg gaatgtggaa ggcaaattac
     9841 tatgtgacag tctcgaataa ccaatttatt agggcctact ccccttctgc agtcgcagtg
     9901 accggatcct ttactagcac gagactgtct ctcgtctcca gcaaaaaagc tagctacaca
     9961 ttagaatgga aagctggaat tttatctagt aactcttatc tagatgctac actgacagat
    10021 aattccatta atattacgta ttagttctca atatattcca cgttagtgga gccgccccaa
    10081 tgaaagatat tactaaaatg tccataggag acaaacaggc ttactgtaaa acagatgact
    10141 gaagcagcaa tcactccttt ataaaaagtg tttgttgctt tatttctttt cagaacaaca
    10201 ggcgcaataa ttagtccaag aggagaaatc aaacagccga ttaaggaaat gagcaataaa
    10261 actaaccctt catctcggtt tgtattattt tttaaataaa ttaatttatt tttagattct
    10321 attatctcta tactattctc ttttatattt tttttaactt cttttttttc ttttaaaaga
    10381 tcatcgatag agatttcata taaactactt aataaagtta agttgtttat atctggggaa
    10441 ctagtatcat tttcccattt agaaatagat tgtctagata tatttaaata ttttgctact
    10501 tcactttgag agatgccttt tatatgccgt gattttttaa tttttcccct aatgtcattc
    10561 tgtatcctct cctattaatg acttttattt attggatatt aagaaataga ttatttagtc
    10621 tatattcaag tgataatcta gttgtttttg aaggatattc tgaacttctt ctaaagattc
    10681 ttcgttaatc atttcatagt atattccgtc atcaagtagt tgtccttccc cctttaattg
    10741 aacttgctct atatttccta aagcagtgga atatttatta ataatagtcc acatatcgtc
    10801 aactgacaga ttagtagtca tatttccctg aagactctta atgagtgaag gtatcttagt
    10861 tgctagtgta gtaactttag ttcctttctc tataattgcc attattattt ccctttgacg
    10921 agcttgtctc ccatagtcac ctgaatcatc tgcatgacga tttcgagagt acgctaagga
    10981 agccttccca tctaagttaa taatttcgcc ttgataaaat gaataactat cttgtgtaaa
    11041 agtggcagtt ggagtaagag ttattcctcc aatagaatca acaatttgtt ctagaccctg
    11101 catatttaac gttacaaaaa agtcaatagg tatatctaag tatgcttgta ttgtatttat
    11161 agtcatggct actccaccaa aggcataggc atggttaatt ttgtcgtaag tatcataacc
    11221 gataatatcg acataacggt tctgttgcaa agttttaaat ctactatcaa ataa
//
LOCUS       DAPBAB010000248         7959 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.100, whole genome shotgun sequence.
ACCESSION   DAPBAB010000248 DAPBAB010000000
VERSION     DAPBAB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.100"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            128..1564
                     /locus_tag="RD444_003190"
     CDS             128..1564
                     /locus_tag="RD444_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7550445.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="RD444_003191"
     CDS             1753..4200
                     /locus_tag="RD444_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDT7550446.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="RD444_003192"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="RD444_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDT7550447.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="RD444_003193"
     CDS             complement(5690..6793)
                     /locus_tag="RD444_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7550448.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD444_003194"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD444_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDT7550449.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAPBAB010000249         7820 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.101, whole genome shotgun sequence.
ACCESSION   DAPBAB010000249 DAPBAB010000000
VERSION     DAPBAB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.101"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(70..1218)
                     /gene="nagA"
                     /locus_tag="RD444_003195"
     CDS             complement(70..1218)
                     /gene="nagA"
                     /locus_tag="RD444_003195"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDT7550450.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1294..2130)
                     /locus_tag="RD444_003196"
     CDS             complement(1294..2130)
                     /locus_tag="RD444_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7550451.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2314..3531)
                     /locus_tag="RD444_003197"
     CDS             complement(2314..3531)
                     /locus_tag="RD444_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7550452.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     ITSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3680..3859)
                     /locus_tag="RD444_003198"
     CDS             complement(3680..3859)
                     /locus_tag="RD444_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550453.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3914..3997
                     /locus_tag="RD444_003199"
     tRNA            3914..3997
                     /locus_tag="RD444_003199"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3948..3950,aa:Leu,seq:gag)
     gene            complement(4237..5607)
                     /locus_tag="RD444_003200"
     CDS             complement(4237..5607)
                     /locus_tag="RD444_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550454.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSLQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6322..6612)
                     /locus_tag="RD444_003201"
     CDS             complement(6322..6612)
                     /locus_tag="RD444_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550455.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6820..7023)
                     /locus_tag="RD444_003202"
     CDS             complement(6820..7023)
                     /locus_tag="RD444_003202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550456.1"
                     /translation="MNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLE
                     KAKDEVEDTADKSQQIVEEHLRK"
     gene            complement(7120..7512)
                     /locus_tag="RD444_003203"
     CDS             complement(7120..7512)
                     /locus_tag="RD444_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDT7550457.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEIN"
BASE COUNT         2393 a         1545 c         1194 g         2688 t
ORIGIN      
        1 ttgcacaact catcagtcct tattgacaaa gagccaagaa gtttagtttt aaggttaact
       61 taaattatat tatgcgtgga aacgttccac gccatctaga taagttgctt ccaatgtcat
      121 atcagggttc aatacgataa agtctgcatc gcgtccttga gcgatttttc cacatgtatc
      181 atcgatttta cagctgattg ctggaatcag actagccatc atgactgctt gttctggtgt
      241 tgctaatccc caatctacaa catttttaat tgcttctttc aatttcaata tacttccagc
      301 taagttgcca gattctagac gtgctgttcc ttcttttact actactggaa attcgcctaa
      361 attgtaattt ccatcaggca tcccaccggc catcatacag tctgtaatta atgcgacatg
      421 atcatgtcct gctttttcca ttaggattcc cgcagcattt ggatgtacat gatggccatc
      481 acaaatcaat tcagaaaaga catgttctaa agataataac gcacccacca tacctggttc
      541 gcgatgattc aatccacgca taccattgaa agcatggaca aatacactgg cgccagcttc
      601 aacagctttt tgcgcttctt ctagcgtagc gttactgtga cctagagaaa caactactcc
      661 ttcttcagtc acttgtttga caaactcttc tacaccttga cgttccggtg ctaaagcaat
      721 ttttttgatt aaaccgcctg aagcttcttg ccattcatgg aaagtatcca aatcaggatc
      781 cccgaaataa cttgggtttt gtgccccttt gtgttcttct gtgaagaaag gaccttcaaa
      841 atagattcct ttgattttag ctcctggtgc ttcctgatac attttgccga ttgtctcagc
      901 tacatctttt aaacgttctt tgcttgacgt caatgttgtt ggtaaaaatg aagtcactcc
      961 acaagataac agaccttcag acatcacttt gatgccttct gcatcattat ccattacatc
     1021 atgattcata tagccgtgaa tatgtgtatc taccaaacca ggagcaatcc atttgccgct
     1081 ttggtcaatg atgttgactt ctccctcagg tagttctttg gtgtattcac caaaaagtcc
     1141 atcggtaatt tcaagatagc ctgcatgttt gacacctgac gctaaaaaga atttgtcagc
     1201 atagataaaa gttctcattc tattctctcc ctttgttttt ctacctctaa agtactctta
     1261 tttagagaag aagtcaagaa tggtctacac catttacaaa ttttttgaaa tttgtttcac
     1321 agcttgtgct aagccttcat agaatgctgc tctttctggt tcttctttac gcttgtatat
     1381 taagctttta agcacaaata aatcattgag gtaatagtag ctattcttct cttgagccca
     1441 atagattcct tgattcacct gttctaacgc ttcgtcgatt tcattttgat ggaataagaa
     1501 atttccataa ttataaaaaa tttgcgaaaa ttcacttttt gatcgttcgt cagctgattg
     1561 gtgcaacagt tgaatgcttc gactcaaata atagcgtgct tcagccgttt ttcctgcaac
     1621 accatatatt tttccaatgc aactgatcag ttgtatttct gtgtcagaaa catggattct
     1681 ttctttttgg ctagagtagc ttaatgcttc tttcaaataa taaagggact gagagatatt
     1741 ttgacgcaac gtgaattcgc aaacaccgag ataataataa tagcgttgaa agtcagttgc
     1801 ttgatacaga ttttcagaaa tatcagaaga ttttagtact tgttctaact tccgataatc
     1861 acgtttttgg aaacaactat ccaacagttc aaataattta tcgtttgtcc gagtagtttt
     1921 tcgagagaga ttcatcacat gatcaatcgt tacccctagc cgttgacaaa tctgcatcat
     1981 caccaataca ttcggcagtt cctcattatt ttcgatccga cttaatacac tttgtgaaca
     2041 aatatcttgc gaaagcattt tttgcgtcat tttttgttct tttcgaatct ctttaatgat
     2101 cgtgccaaag gactcgatta acgtttccat aagtatcctc cccaaaatat gcgtattcgc
     2161 attctattcg tattttactt taggaagtcg caaacttcaa gaaaagactt gcaactaaat
     2221 aatatacttt agttatttaa gaaaactcca ctagaaagag agcctcagtg tgtttgtctg
     2281 tggccctcct actgaaaaat ttggttttta aaatcatttt tggcgatagg ttgctaggaa
     2341 ttctctcatt ttcttagctg tttgtttcaa atcatccatt ttaggaagat aaacgattcg
     2401 aaaatgatct ggctggttcc aattaaatcc accaccatgt acaagtagaa tatgatgttc
     2461 atgtaaaaag tccaaaacga atttttcatc atccaaaata ttgaatcgct ttgtatcaat
     2521 ttttgggaat atataaaaag cagcttttgg ttttacggct gaaagaccag gaatatcatt
     2581 gattgcatta tagatatatt ctctttgttc atagatcctt ccgcctggca ggagcagctc
     2641 atcgacactt tgataaccgc ctagcgcggt ttggataatt tgctgagaca atacattgga
     2701 acacaaacgc atggaggaca acatattcag cccttctata tagcctttaa cgtgtgactt
     2761 atctccactt aggaccatcc agcctactcg aaatcctgct acacgatgag atttggatag
     2821 accattcagc gtaacaacga acaaatcagg cgctaatgta gcaatcggaa tatgttctag
     2881 accatccatc actaagcgat catagatttc gtcggaataa ataatcaggt tattttgtcg
     2941 agctacttca acgatttctt ctaataattc ttttggataa agtgccccag taggattatt
     3001 aggattaatg ataacaatag cttttgtccg actcgttatt tttgatgtga tatcatcaat
     3061 atcaggatac cattctgcct cttcatcgca aatataatga acaggtgtgc ctccagctaa
     3121 tgcaatagaa gccgtccaca acggatagtc cggcattgga accagtactt catctccatt
     3181 gtccagcaac ccctgcatac acatcgtgat caattcgctc actccgttgc cggtgtagat
     3241 atcattgatc gtcacattcg gaaaatgttt cagttggcaa tattgctcaa ttgcttttct
     3301 agcagaaaag atccccttgg aatctgagta gccttctgag tcacgggcat tcatgatcaa
     3361 atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt gcgggatttc ccgtgttcaa
     3421 ttttaaaata ctaatgccct cttcttgcat ccgctccgct tcttctagaa cgggaccgcg
     3481 aacatcgtaa ctgacacctt ctaatttgtt ggatttttca aattctctca tcacttagcc
     3541 aatccctcat ttcaaaaagt tagaactttc caaatttttt gttatttact atttcaatat
     3601 catactccat cctagaaaat tttgccactt atttaggaaa aacgtaagta ttaaaaaaac
     3661 acaaacaagc gttaagaact taattgtcat gatagtcatt ccatagattc gctgcccaat
     3721 ccattgcaat tccacaaatg attcctagca taaaaccaag caaaaaacgt ccgaatgcaa
     3781 gtccaatcaa tacacctaag atcattccca gtcctgaaaa ttctccgtca tatttctcca
     3841 tcttaataag tcctcccatt tttgagcttg acaactatgt aaaacctcgc tatcattatt
     3901 gtgttattat tacgcagatg tgttggaatt ggcagacaag catgattgag ggtcatgtga
     3961 gcgttagctc gtgtgggttc gagtcccatc atctgcacta caactcgtga agttaatttt
     4021 gcttcacgag tttttttgtt tgaacgtttc tttatgccat tatatacata tcactgtatc
     4081 ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg tgagtatatg aaggtcggaa
     4141 acaagctact cgtagaaact tgcaagtgtt aaaacaatta atcgaaaaac gagtaaaatc
     4201 cctcacctaa tactagagcg agggattttt tgtatattat ctgtttatca ccgttattat
     4261 aaaggtacaa ataccaagca cggttaatcc aaattgcata aattcatttt tttgtaaact
     4321 catgcttttc attagctctt taactgccat tagtactaag atggcgataa caacataaag
     4381 tatatagtac gtccgcattc cggaagccca tactgtggga gaaaatccca taattaacct
     4441 tgaacaaaat cccattatca tcaaaataac taaaaaaata gaagttttct tattgtcaaa
     4501 acttaaataa attccaacaa ttaaacatag taataatgcc aagactacta aagtagctat
     4561 ccaagtacct ggataaaaaa gtgacagttt cgttcctaac tctgtaaaga gattattcag
     4621 gttactacta ctccaaatca ttgccctttt atttccattg acgtaagtaa agctttgacc
     4681 cattgtatta ccaaaataaa taatcaggtt taagaaaaac ggtaatgcag tcaatatacg
     4741 cacataatag ttttgacatt tcctatatgt tagaaggaaa attaagaaaa atagcaataa
     4801 aaataagata ttcatgtcta aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa
     4861 cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc
     4921 acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga
     4981 aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac taactatact
     5041 tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg
     5101 aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg
     5161 ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca
     5221 accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa
     5281 atttttaaca tttattttct caaaaacata cttgcaaagt aatactacaa ttcctaacat
     5341 aatcgtagaa tttaataatt tccatagcat aaagtgttta gaaaagaaca ttaagaatag
     5401 ttctataata attcttgaac tccatgtctt ataatgttct atcgaagcac ccaaatagcc
     5461 aagttcatct aatctacttg cataaactac gtcatccgct ctcatagtcg gcatatacat
     5521 attaaagagt aaaaagatac cccaaatccc tattagtaac aagatcagtt ctttgttatt
     5581 cgttattttt ctattatgtt tgttcatttt tatactcctt cttatatttc ctaatagagt
     5641 ataacatagc tttaagcatt acttaaagat aacttaaaag ttatctttta aatacttaat
     5701 tgttaaatac cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa aaattttcat
     5761 tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt
     5821 cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag
     5881 attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg
     5941 tgaaagacga ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac
     6001 ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat
     6061 gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga
     6121 attgatctgt tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc
     6181 atcacaaata cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct
     6241 cgtgtgggtt caaatcccat cgtctgaatc catctttcca aaacaagcgg ctaggacatt
     6301 tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg
     6361 gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct
     6421 cacaacaaat tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc
     6481 tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact
     6541 gaaacggatc aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata
     6601 ttttttctcc ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag
     6661 aagaaactct gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg
     6721 cacttcctgc gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagattactg
     6781 ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca aatgttcttc
     6841 aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt tttctaactt
     6901 ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt tcttttgtgt
     6961 atctgttaac tgagtttcta catttgtttt ttcttgtgtc agtgttgcga tttgttgatt
     7021 caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt gtagtcttgc
     7081 tgtccaatct gcattcagat gattgatttc atcatttttt tagttgatct catctagttt
     7141 gacattgatt tcattttgct tgctttgtag ttgtgcatcc ttgctgacta attgactttc
     7201 taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat ttacttttgt
     7261 ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg
     7321 agcggtaaga tctgtaatcg cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag
     7381 attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt catttgcgta
     7441 ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac cagtaataaa
     7501 tttctttttc atttgcatag ctccttaatc caatactttt acatactaaa agtaagtgat
     7561 ctcaaacaca atgtcaagcg tttttaaatt acatatgtaa ttttaatata aatactatca
     7621 aatactcatt taatcctcct tttttatcta tttaaaaaat cttttttata gagaaaaatg
     7681 caggttaata ctttcttaaa taaatattta tggtgaacaa aaacaaaaaa gcaagtttta
     7741 ttaaacgtaa cccttttcat ttcatagatt actgtattta ttacaaaaaa cgtggatttg
     7801 tcataaattg tcatgcttct
//
LOCUS       DAPBAB010000250         6931 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.110, whole genome shotgun sequence.
ACCESSION   DAPBAB010000250 DAPBAB010000000
VERSION     DAPBAB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.110"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            160..1080
                     /locus_tag="RD444_003204"
     CDS             160..1080
                     /locus_tag="RD444_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7550458.1"
                     /translation="MKKNVLDIKDLSLKYGKKFAIKDINLSIFTGEIVALVGHNGAGK
                     SSLFNIIAGIQTPTTGSVEFDQDFIEKRLFATIGFSPQTQVMDWYTNVWDNVYLGSQL
                     AGIDAKTGKLLTEKALDLLSISDLKNKPVDALSGGQQQRVQIARSIVHQPDFYLLDEP
                     TTGLDAETSNRLLSFLKKEAAKGKSVFVSSHDLHLLEEFCDNLVLIDKGRVLYAGDLK
                     NFLIENAPKKSYQITTDRSIHEEIIHKLNEKGIAVTGLDNFQYRFILNQESDLTQLFM
                     DTKEFFRIDEIVKETMSLRDLYIHYREGGL"
     gene            1082..1888
                     /locus_tag="RD444_003205"
     CDS             1082..1888
                     /locus_tag="RD444_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7550459.1"
                     /translation="MMPSQFREQHYLGIKGLKIIVKNELNAFLRNKGLIISQLVQPIL
                     YYIFIVLGINSFVTNIAYEGKTISYADYALTGILGILIISQMTQTIYRITIDKRYGLL
                     ALKRQSGVSPFYYIIGMSMYPTIGICIQSSVLYIIATLFGSSIGLINYLAGIIVVIIV
                     LYFWTSIAALITMKINDYQRRDSIISFIITPLGFTAPAFYLITNVPTFIKVIGYFNPL
                     TYQLYAIRSIVFATPANSLLIIVLVFLATLIMISISSFAVSKMSLVLTER"
     gene            1900..3111
                     /locus_tag="RD444_003206"
     CDS             1900..3111
                     /locus_tag="RD444_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDT7550460.1"
                     /translation="MRILSIFFTNDRKCIIASMLSKTGNYRLYYSLYPFEKKDMICER
                     SFMIVPIGIKKGKLIYKEKNQVNHFYVPNCKASGFLNNVRSIDYNTLLHDTYKNLSLT
                     VTNDKGGNIVFVNQEKLNIPVHFTVKNCYFLDNKTLILLCSSTLNSSMLVYYSIPERE
                     LNYLKMTSQLTFPLSFFEHQIIGEKKIPCLTHINRSDDTVLICLHGGPKYHYNHEYLD
                     LIFDFYHNFNNICLVNFPGSTGYSQKYENELIGNGGVVDVEAVKSVVSYYDSQGFKIK
                     IYGESYGAYIAICLSQFINFSIARIVSVSGFTNLFYMYLCSDSRNLIEKYFSDYRKFS
                     PEKNLAMKELLPIRFLHGSEDKTCPIAQINYFVKKYEGMHLTVLDGFSHYEVDIKKEK
                     IRNKRIVRLLK"
     gene            3163..3396
                     /locus_tag="RD444_003207"
     CDS             3163..3396
                     /locus_tag="RD444_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550461.1"
                     /translation="MSKELKKLVADLQGNMDVVGEFMDSPQSVLDKYNVQGKERTVML
                     AKDSEGLNKLGFSSQLVEGALSGAHSQRCHAPI"
     gene            3732..4379
                     /locus_tag="RD444_003208"
     CDS             3732..4379
                     /locus_tag="RD444_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-8 chloramphenicol O-acetyltransferase"
                     /protein_id="HDT7550462.1"
                     /translation="MTFNIINLETWDRKEYFNHYFNQQTTYSVTKEFDITLLKSMIKN
                     KGYELYPALIYTIVNIINQNKVFRTGINSEGNLGYWEKLNPLYTVFNKETEKFSNIWT
                     ESNVSFNSFYNSYKSDLLEYKDKNEMFPKKPIPENTVPISMIPWIDFSSFNLNIGNNS
                     RFLLPIITIGKFYSKNNKIYLPVSLQVHHAVCDGYHVSLFMSEFQNIVDSVNEWI"
     gene            4632..5843
                     /locus_tag="RD444_003209"
     CDS             4632..5843
                     /locus_tag="RD444_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDT7550463.1"
                     /translation="MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNY
                     DLVNRTENYKRDIEQFINDNKSSSRAVRKDAVLINEWIITSDNPFFKDKDDKEIKDFF
                     DTAKSYFADKFGDNNIRYAQVHLDETTPHMHLGIVPFNDEHKLSAKTVFNRQALQAVQ
                     DELPKYLNERGFELERGEKGSERKNLTVPEYKKAKDELKEITTTLEQRKSEVLALSND
                     KAPTIKKESLDLKDETKTVKVPSGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVI
                     KSYEELYKANEKLKNYAETDLPKEITRVKEKYRELAKDYNRLVRKSNQNIETLEELEK
                     ENKSLKNEIKGIYKGFNQFMENTLGATVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNE
                     TKTRDRDELSR"
     gene            6387..6542
                     /locus_tag="RD444_003210"
     CDS             6387..6542
                     /locus_tag="RD444_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein repA"
                     /protein_id="HDT7550464.1"
                     /translation="MVEVEKKKVTLSLPVESNDKLEKMAQKYGMTKSGLVTFLINQAD
                     DKGTIFK"
     gene            6666..>6931
                     /locus_tag="RD444_003211"
     CDS             6666..>6931
                     /locus_tag="RD444_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HDT7550465.1"
                     /translation="MENENRTDWTLPRKNLNPKTKQPYKRGRNWWIVVYPESLPENWK
                     EIISTEPVAISPLHDKDVNADGTKKKPHYHIVFNRIRQKSPILY"
BASE COUNT         2521 a          873 c         1292 g         2245 t
ORIGIN      
        1 aggaggtttg gggtgtgaga cttcgattat ccttatagga atcaataaat ttttttaaaa
       61 cattctgaat tttctgatcg atggttcgtt cagccatatt agaatccctc tcttttaagt
      121 aagatttaat taattatacc ttagaaaagg agtttagtaa tgaaaaaaaa tgtattagat
      181 ataaaagatt taagtcttaa atatggaaaa aaatttgcga taaaagacat taatctttcc
      241 atatttaccg gtgaaatagt agcattagtt gggcataatg gagcaggtaa atcaagctta
      301 ttcaatatta ttgctgggat tcaaactccg acgacgggtt cagtagagtt tgatcaggat
      361 tttatagaaa aacgattatt tgcaactata ggtttttcac cgcagactca ggtgatggat
      421 tggtacacaa atgtgtggga taacgtttac ttaggcagtc aattagcagg aatagatgca
      481 aaaacaggaa aattattgac cgaaaaagcc ttagatttat taagtatatc ggatttaaaa
      541 aataaacctg tagatgcact atctggggga cagcaacaac gtgttcaaat tgcgcgctcc
      601 attgttcacc agcctgactt ctatttgtta gatgagccaa caacaggact agatgcagaa
      661 acatctaatc ggttgttgtc atttttaaaa aaagaagcag ctaaagggaa atcagttttt
      721 gtgtcgtcac atgacttaca tttattagaa gaattttgtg ataatttagt tttgatagat
      781 aaaggaaggg ttttatacgc gggggatcta aaaaatttct tgatcgaaaa cgcgcctaaa
      841 aaaagttacc agattactac tgatagatca atccatgaag aaattattca taagttaaat
      901 gaaaaaggta ttgctgtgac aggattagac aactttcagt atagatttat attaaatcag
      961 gaaagtgatt taacacaatt gtttatggat acaaaagaat tctttagaat agatgaaatt
     1021 gttaaggaaa ctatgtcatt aagagactta tacattcatt atagagaagg gggtctttaa
     1081 aatgatgcca agtcaattta gggagcaaca ttatttagga ataaagggat taaaaatcat
     1141 tgtaaaaaat gagctaaatg cgtttcttag aaacaaagga ttgatcatat cacaattggt
     1201 tcaaccgatc ttgtactata tttttattgt cttaggtatc aatagttttg tcacaaatat
     1261 tgcctatgag gggaaaacaa tttcttatgc cgattatgct ttaactggga ttttgggaat
     1321 attgattatt tcacaaatga ctcaaacaat ttatcgaatt actatagata aaagatatgg
     1381 tctattagct ttgaaacgtc aaagtggagt ttcaccattt tattacatta taggaatgag
     1441 tatgtatcct actataggca tatgtattca gtctagtgta ctttatatta tagctacttt
     1501 gtttggaagc tcaattggtt taatcaatta tttggcaggg ataatagttg tgataattgt
     1561 cttgtatttt tggacatcca tagctgcgtt gattacgatg aaaattaatg attatcagcg
     1621 aagagattca attatttcgt ttattattac tccattaggg tttacagctc ctgcatttta
     1681 tttaattaca aatgtcccta catttattaa agttattggt tactttaatc cgttaactta
     1741 tcaactatat gcaataaggt ctatagtatt tgctacacca gcaaatagtt tattaatcat
     1801 cgttttagta tttttagcca cattgattat gataagtatt tcgtcatttg ctgtttccaa
     1861 gatgagttta gtgctgacag aacgatagaa ggtgactgag tgagaatcct aagcattttt
     1921 tttactaatg atcgaaaatg tataattgca agtatgctaa gtaaaacagg taattataga
     1981 ctttattatt cgctgtatcc atttgaaaag aaagatatga tatgcgaaag atcttttatg
     2041 atagtgccaa taggtataaa aaaaggaaag cttatctata aagaaaaaaa tcaagtgaat
     2101 catttttatg tgcctaattg taaagcctct ggtttcttaa ataacgtcag atcaattgat
     2161 tataatactc tgttgcatga tacttataaa aatttgagtt taactgttac gaatgataaa
     2221 ggaggcaata ttgtatttgt aaaccaagaa aaattaaata ttccagtaca ttttactgta
     2281 aaaaattgtt attttcttga taacaaaaca ttgattcttt tatgtagttc aactttgaat
     2341 agctcaatgc tagtttatta cagtattcct gaaagagaat taaattattt aaagatgact
     2401 agtcaactta ctttccctct ttcttttttt gaacatcaaa taattggtga aaaaaaaatt
     2461 ccttgtttaa ctcacattaa tagaagtgat gatacagttc tgatatgttt gcatggagga
     2521 cctaaatatc actataatca tgaatactta gatttaatct ttgatttcta tcataatttc
     2581 aataatattt gtttggtgaa ttttcctgga agtacaggat attcccaaaa atatgaaaat
     2641 gagttaatag gtaacggagg tgttgttgat gttgaagctg taaaatctgt cgttagctat
     2701 tatgattctc aaggatttaa aataaaaata tatggtgaga gttatggagc gtatatcgca
     2761 atctgtctct ctcaatttat taattttagt atagcaagaa tagtctctgt tagtggattt
     2821 acaaatttat tttatatgta tctttgttca gattcgagaa accttattga aaagtatttt
     2881 tctgattata ggaaattttc tccagaaaaa aatctggcaa tgaaagaatt attacctata
     2941 agatttcttc atggaagtga agataaaact tgtccgattg cacaaattaa ctattttgta
     3001 aaaaaatacg aaggaatgca tttgacagta ctagatggtt tttctcatta tgaagtagat
     3061 ataaaaaaag aaaaaatcag aaataaaaga atagtcagat tattgaagta attactgatg
     3121 aatgttatca tataattata aactttacgg gggtgcagta ttatgtctaa agaattgaaa
     3181 aaactggttg cagatttaca gggaaatatg gatgttgtcg gtgaatttat ggatagtcct
     3241 caaagtgtct tggataaata caatgtacaa ggtaaagaaa gaactgtgat gttggcaaaa
     3301 gatagcgaag gtttgaataa actagggttt tcgagtcagt tagttgaagg agctttatct
     3361 ggtgctcaca gtcaaagatg tcatgcacca atataaaaat atttccatat tcctctactt
     3421 tatttatgta gagggatatt attgtgctaa tagcattgtt aagtcaatca attatactta
     3481 aatagtctga tttatttgca aagttgaagt ttaatgaaat cgactttaaa agcgaacgaa
     3541 aaacaattgc aaaagcagat tgatttttgg caacgtgaat ttagattttg gaagtaaagt
     3601 aactttgttt tatacaaaat gttactttag tataattaac gtttaagagg tgacacaata
     3661 tatgaaaaaa tcagaggatt attcctccta aatacaaaga gataagatga aatttaggag
     3721 gaaaattata tatgactttt aatattatta atttggaaac ttgggataga aaagaatatt
     3781 ttaatcatta tttcaatcaa caaacaactt acagtgttac taaagaattt gatatcactt
     3841 tacttaaaag tatgataaaa aataaaggat atgaactgta tcctgctttg atttatacaa
     3901 ttgtaaatat tataaatcaa aataaagtat ttagaacagg aattaatagt gagggaaatt
     3961 tgggttattg ggaaaaatta aaccctttat atacagtctt taataaagaa actgaaaaat
     4021 tttctaacat ttggacagaa tcaaatgtta gttttaattc tttttataat agttataaga
     4081 gtgacttact tgaatataaa gataaaaatg aaatgtttcc taaaaaacca atacctgaaa
     4141 acacagttcc tatttcgatg attccttgga ttgattttag ttcatttaat ttaaatattg
     4201 gtaataatag tagattccta ttgccaatta ttacaatagg taaattttat agtaagaata
     4261 ataagatcta tttaccagtc tcattgcaag ttcatcatgc ggtatgtgat ggttaccatg
     4321 tttcattatt tatgagtgaa tttcaaaata tagttgatag tgtaaatgaa tggatttaaa
     4381 tctaaaataa ctttcataaa cttaagtaat tatgatattt ataagtttat agcaactacc
     4441 atttttggtt gctggtgttt tgattttgaa tttggttttg aacttatgga ctgatttatt
     4501 cagtccattt tttgtgcttg cacaaaaact agcctcgcag agcacacgca ttaatgactt
     4561 atgaaacgta gtaaataagt ctagtgggtt atactttaca tggaagttgt gccgaagtta
     4621 ggagtgttta catgtcattt gcagtggcga gaatgcagaa ggtaaaatca ggaaatttag
     4681 tcggggtagg taatcataat cagagaaata cagacaatca ttccaacaaa gatattgatg
     4741 ttgaacggtc gcatttaaat tatgatttgg tcaatcggac agaaaattat aaacgagata
     4801 ttgagcaatt tattaacgac aataaatcaa gtagtcgtgc tgtcagaaaa gatgctgtat
     4861 taataaacga atggatcata acttctgaca acccattttt taaagataaa gacgataaag
     4921 aaattaaaga tttttttgat actgcgaaat catattttgc ggacaaattt ggcgataata
     4981 atatccgcta cgctcaagtt cacttagacg aaacgacacc gcatatgcac ttaggaattg
     5041 tgccgtttaa tgacgaacac aagctatcag caaagactgt ttttaatcgt caagccttac
     5101 aagcggtaca agacgaattg ccaaagtatt taaatgagcg tggttttgag cttgaacgtg
     5161 gcgaaaaagg gtcagagcgt aaaaatttaa cggtgcctga atataaaaag gcaaaggacg
     5221 aattaaaaga gataactacc acgctggaac agcgaaagtc agaagtttta gcgttatcga
     5281 acgataaagc accaacaatc aagaaagaaa gtttagattt aaaagacgaa acgaaaacgg
     5341 tcaaagtacc aagtggcgaa accattggga ttggtaagct gcgacatgaa tttatgaaaa
     5401 atgaagaacg aaaaacggga aatgtaatta tccctgaaag taaattaaat gcagtgatta
     5461 aaagctatga agagctatat aaagcaaatg aaaagctaaa aaattatgcg gaaactgatt
     5521 tacctaaaga aatcactaga gttaaagaga aatatcgcga attagcgaaa gattacaata
     5581 ggttggtacg taagagtaat cagaatattg aaacacttga agaattagag aaagagaata
     5641 agtcgcttaa aaacgaaatt aagggcattt acaaaggttt taaccagttt atggaaaata
     5701 cccttggtgc aactgttgga caagctaaaa agctgatgaa taaccttatg agcgaagtta
     5761 aagagtttgt aaaaggtgga gagtttgaaa aaattcatag aaacgaaact aaaacacgag
     5821 atagggatga attaagtcgt tagaagctca tatttgcgtt ctaagcgctt ttttatcaaa
     5881 actagggtaa ttctgcatat tgtttacttt tcgtcggcaa atgcgtaaaa aacagcgaaa
     5941 taaaagaggt caagggcgaa cgtagtgagt gtatttaccc ttgatctctt tttttgagtt
     6001 gtttagcttt tgctaagacg acggaaagaa acgaatgcag ttatcaagtt gcttttcaga
     6061 ggcaaggtca agcgctttgc gcttgttggg tgtgggcgaa tagcccacgg ttttattatt
     6121 tggaaattta gtgggagcag ggggcgaaaa aacatgggaa tttttggagc gaaaaaacat
     6181 gggggcacta cgacaccccc catgtgtcca ttgtccattg tccaaataga taaaaagatt
     6241 aaatgtcttg taaatacctt tatatgaacg ttttaagcac ctatttcgag gtgaaaaaaa
     6301 gttttttgta aaatcttgta ttcctgtcgt atgtataata taattacagt gtaaaagaga
     6361 ttttgaagag aattgaggtg cattttgtgg ttgaagttga gaagaaaaaa gtgactttat
     6421 ctttacctgt tgaatcgaat gacaaactag aaaaaatggc acaaaaatat ggaatgacta
     6481 aatcaggttt ggttacattt ttaattaatc aagctgatga taaggggact atttttaaat
     6541 aaaaaaagcc ctgctaggaa gatagcaagg gctcgcaaga tttggttttc tttgatttcg
     6601 caactcaagt ttatcaaatt ttcccttgtt ttcctagtag agtaactagg aaggtgtaga
     6661 aaaatatgga aaatgaaaat agaacagatt ggacattgcc tagaaagaat ttaaatccaa
     6721 aaactaaaca gccttataag cgaggtcgta actggtggat tgttgtttat cctgaaagtt
     6781 tgccagagaa ttggaaagag attatttcaa ctgaacctgt ggcaattagt ccattacatg
     6841 ataaagatgt taatgcagac ggaacaaaga aaaagccgca ttatcatata gtttttaaca
     6901 gaattaggca gaagtcgccc atcctctata g
//
LOCUS       DAPBAB010000251         5737 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.122, whole genome shotgun sequence.
ACCESSION   DAPBAB010000251 DAPBAB010000000
VERSION     DAPBAB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.122"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            complement(86..3010)
                     /locus_tag="RD444_003212"
     CDS             complement(86..3010)
                     /locus_tag="RD444_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDT7550466.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3156..3731
                     /locus_tag="RD444_003213"
     CDS             3156..3731
                     /locus_tag="RD444_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7550467.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3945..4640
                     /gene="vanR"
                     /locus_tag="RD444_003214"
     CDS             3945..4640
                     /gene="vanR"
                     /locus_tag="RD444_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDT7550468.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4618..>5737
                     /locus_tag="RD444_003215"
     CDS             4618..>5737
                     /locus_tag="RD444_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7550469.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELP"
BASE COUNT         1767 a         1085 c         1084 g         1801 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt ctaaaatact ttgttttttt
       61 acctgtctac ttgacagggg gcagttcact ctgagttaaa atggtattct cctagtaaat
      121 taatatgttc ccaacctaag ggcgacatat ggtgtaacaa atcttcatta aagctacctg
      181 tccgtttttt atattcaact gctgttgtta ggtggagagt attccaaata cttatagcat
      241 tgataattat gtttaaagca ctggctcttt gcaattgatg ctgtatggtg cgttctctaa
      301 gctcaccttg ttttccgaag aaaatagctc ttgccaatcc attcatggct tctcctttat
      361 tcaatcctct ttgtattttt cttcttaatg attcatccga tatataattc aaaataaaga
      421 tcgttttttc tattcggccc atctcacgta aggctgtagc taagctgttt tgtcttgaat
      481 aggaacctag cttccccata ataagggatg ctgaaactgt tccctccctt atagaatgag
      541 ctaatcgcaa aacatcctca taattttctt taatgacctt tgtatttatt tgtccacgta
      601 aaatggcttc tagttttgga tactcacttg ctttatctat cgtaaataat tttgagtccg
      661 ataaatccct tattcttggg gcaaatttaa atcctaataa atgagtcagt ccgaatattt
      721 ggtcagtgta accggcagtg tctgtataat gttcctctat gtttagatcc gtctcatgat
      781 gtaacaaacc atccaaaaca tgaatcgcat ctcttgaatt agtatgaata atctttgtgt
      841 agtaagaaga gaattgatca cttgtaaatc ggtagatggt ggctcctttt ccagttccat
      901 aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc ataccatctg
      961 acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga tgaaagttta
     1021 ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga gatacattgg
     1081 ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag ccaatattca
     1141 ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct ggttttcgat
     1201 tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca tccatgagta
     1261 aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat ttttttgctt
     1321 cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca agagaaaccc
     1381 catctaactt attggaattg gcagctaacc actttaacct ttcattaaag ctgctggttc
     1441 tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc cccttcgatt
     1501 gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt ctgctgccaa
     1561 caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca gccatttcat
     1621 agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc catcgttttg
     1681 aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg ttcattcctc
     1741 ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga aattccaata
     1801 ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc agtaagtcta
     1861 aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa gagacaaagg
     1921 tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc tctcttgctt
     1981 taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc tttttaccgt
     2041 tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta agtatttgcc
     2101 tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat aaatagatgg
     2161 ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc tcgtatcttg
     2221 agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt tgcactgttt
     2281 ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa gtttcgggtg
     2341 aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat tcggatggat
     2401 gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga gatttactaa
     2461 ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt ctttcaagtg
     2521 tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat tcatgcagta
     2581 gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat gtcattcgat
     2641 attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc aaatgatccc
     2701 aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca ccaatctgtt
     2761 tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat ggccaaccgg
     2821 gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc ctccttcgct
     2881 tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt atccattcat
     2941 cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct ctacctctcg
     3001 caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc aattatatat
     3061 caatagagtg tactctattg atacaaatgt agtagactga taaaatcata gttaagagcg
     3121 tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt tatattcgtg
     3181 tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag atcggaatgg
     3241 atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa cttcaaaaag
     3301 tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact cgaatcactc
     3361 gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag gcaagtttaa
     3421 aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc caattcttaa
     3481 ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg agacaacgtg
     3541 aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag aagtatcata
     3601 aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat atgactgtaa
     3661 atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa ttatcagaag
     3721 tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa gaaaaggaaa
     3781 ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa aaaagaatca
     3841 tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg gccctataat
     3901 ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc gataaaatac
     3961 ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta aaaaacgaga
     4021 attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata gacaagtctg
     4081 agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt actatctgtc
     4141 aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa gatacagagg
     4201 tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag ccctttcgcc
     4261 cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa ttcagtggag
     4321 taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat gttaacaccc
     4381 atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt tcaatactgc
     4441 gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt catgagatat
     4501 ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc cggcatttgc
     4561 gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta tggggggttg
     4621 gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa actttacatg
     4681 tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg ttcaatgatc
     4741 cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga cttaaatcac
     4801 ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat ctttatttat
     4861 gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa attcgcaaaa
     4921 tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga taaacaaatt
     4981 gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa acggactctg
     5041 gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt tgttatgtac
     5101 ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag cctgcttgac
     5161 gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac gttggacaaa
     5221 gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta taacctacaa
     5281 acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca gatgaccgat
     5341 gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc ccccgaggat
     5401 ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat tttgaaaaac
     5461 gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct ctccggggat
     5521 gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct agctgccata
     5581 tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg cgcgggactt
     5641 ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta cgcggaaagc
     5701 aatgataact atacgacgtt tagggtagag cttccag
//
LOCUS       DAPBAB010000252         5212 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.132, whole genome shotgun sequence.
ACCESSION   DAPBAB010000252 DAPBAB010000000
VERSION     DAPBAB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.132"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..528
                     /locus_tag="RD444_003216"
     CDS             <1..528
                     /locus_tag="RD444_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HDT7550470.1"
                     /translation="RDFILYLKLCNRTDSGYIPDTKAMLIYDETGQLENYLSEISGAR
                     PNRLTEENANFLLCNFSNLWLMGINVLKRGEYARSLELLSQLQKNTLQLIRMAEKNAD
                     NWLNMSKNLEKEISLENYKKFAKTTARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLK
                     VTHDILERLLNYISE"
     gene            1216..2037
                     /locus_tag="RD444_003217"
                     /pseudo
     CDS             1216..2037
                     /locus_tag="RD444_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            2180..2404
                     /locus_tag="RD444_003218"
     CDS             2180..2404
                     /locus_tag="RD444_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7550471.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            2419..3288
                     /locus_tag="RD444_003219"
     CDS             2419..3288
                     /locus_tag="RD444_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDT7550472.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3269..4006
                     /locus_tag="RD444_003220"
     CDS             3269..4006
                     /locus_tag="RD444_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7550473.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVD
                     FPEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYN
                     EKGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPP
                     ENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4039..4947
                     /gene="ant(6)"
                     /locus_tag="RD444_003221"
     CDS             4039..4947
                     /gene="ant(6)"
                     /locus_tag="RD444_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDT7550474.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKGLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4944..5192
                     /locus_tag="RD444_003222"
     CDS             4944..5192
                     /locus_tag="RD444_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HDT7550475.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1796 a          772 c         1121 g         1523 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg attcaggtta tattcctgat
       61 acgaaggcta tgcttattta cgatgaaaca gggcaattag aaaattattt atcagagata
      121 agtggtgcaa gaccaaatag acttactgaa gaaaatgcta attttttgtt gtgtaatttc
      181 tctaatctat ggttgatggg aatcaacgtt ctaaaaagag gagaatatgc tcgttcatta
      241 gaactcttat cacaacttca aaaaaataca ctacaactta tacgtatggc agaaaaaaat
      301 gctgataatt ggctaaacat gagtaaaaac cttgaaaaag aaattagcct tgaaaattat
      361 aaaaaatttg caaagaccac tgctcgatta gataaggtag aattatttga agcctataaa
      421 aattctttgc tattagttat ggatttgcaa agtcacctta ttgaacaata caacttaaaa
      481 gttacacatg acattttaga aagattgttg aattacatta gtgaatagga gagtaacgtt
      541 attcacagta ttgggtactg aaaattaaca gcatacctca gtttttcaaa aaacagcgaa
      601 gcctatggat atgttcccgt ctaccgatag tgcaaaaagt gtcgtggata tgtttccgag
      661 aacggacata cacaaatgac attcattctg tcctctcaat ccatgtggaa gcagtatgtc
      721 tgttaacaag aaaataatta ttgttcgtcc tgacgagttg ttagactcga aaaggataaa
      781 catcattttt agaaccggta gccgattttt ggctagcggt tttttgggct ctttggcaaa
      841 tagtggtgat tgaatctcgc gctatcctat ggaaaaccgc cttgtgtgag gggctgcgag
      901 accgcttcga tcctagaatt gctgaagcac gaatctagcg tcactcacat ttcatctttt
      961 gacgtagctg agacaaggtg tttttcttcg atccgctagt ggttaatctc tttttggtgc
     1021 aaatatggtt tagaatttct ttgcatggct taagggagtg gagaatcgga tattagtagg
     1081 atttacatcc acagataaaa ataggcttga ttttaacagc gaaactaaaa taccctactt
     1141 aaatgaagtg atagattaga tgttgggttg ttaggagatg cttaaaaatc ctatgcaatc
     1201 aatagttttt agtgtttgtt gacttgatcc ctaaatcgcc tgagtactaa acgttgagtg
     1261 tgtctgctta ctgtcactag ccagtaagtg agactgtagt taatctctca ttttccccag
     1321 tattatgttg aaaatagcca tgaagcgatt atttctaaag agttattttt gcaggctcaa
     1381 gaagaaattt atcggagaag taatatctac acaggagaag gcaagaacaa aagaatttat
     1441 agtagcaagt acgctttaag tgccatcacc ttctgtggat attgtggcga tatttatagg
     1501 agaacctatt ggaatattca tggcagaaaa gaatttgtct ggcgatgcgt gactagaatc
     1561 gagcagggtc ccgaggtatg taagaaccga accgtaaaag aagatgaact ctatggtgcg
     1621 gtaatgaccg caattaataa gctacttgct ggtggcaaca atatgataaa gacactggaa
     1681 gaaaatattc atgctgtgat tggagaaacc acagaatacc aaatttcaga gattaatacc
     1741 ctactagatg aaaaacaaaa agaactaatc aagctggcca ataaaggtca agattatgaa
     1801 tacctggtag atgagatcga tgagatgcga gataagcgtc agactctttt agtagaagat
     1861 gcctccctca gtggcgagaa cgagcgcatc aatgagttga ttgaatttat ccgcaagaac
     1921 aaattccgca ccttagaata tgatgataag cttgtaagga agataatcca gaacgttaag
     1981 gtctatgaag accacttcgt catagccttc aaaccaggta tcgaaatgga aatatgaaaa
     2041 ccagaagtaa atagcccatg actctgaagt agagttgtgg gttttctttt aattgctgat
     2101 tataataata ttttaatgac tattgcattt atcaaccaaa tggtttatgt tattcttagc
     2161 ataaacttgg aggtgctgta tgactacggc agaaatgatt aaagaactgt gtgagcaaat
     2221 gaatataagt gtttccgaac ttgctagacg tattggccag actccacaga attttaataa
     2281 aaaattacaa cgagaaacag taaccttgga tgagttgaag gccattgctg atgtgctagg
     2341 tgtcaagttt gtgcaggcat ttattttacc agatggagat gaaataaaaa tatctaacga
     2401 ataaaggagg atgtttatat ggtaggtaat gttattcaaa tagtaacaga gaaattatca
     2461 tctttgcctt ttatagaagg cattgtatta gggggctcac gtgcaagaag cacccataca
     2521 gaaaattctg atatagatat cggaatctat tacaattcag actcatttga cctgacagcg
     2581 attaatcaaa ttgctacaga gttggatgat gagaatagaa acaaccttgt tgtacctccc
     2641 ggagcgtggg gtgattgggt taatggcggt ggatggttag ttataaacgg gtgtcatgtt
     2701 gacttgatat tacgtgatat aaaacgggtg gaacaaataa tcaaagatac ggagcaagga
     2761 attgttactg ccaattatca gaccgggcat ccccatggtt atataagtgc tatgtatcgt
     2821 ggagaattag cgattagtaa aatactatat gctaagaacg aaagcttatg cgaattaaaa
     2881 aagcaggcag aaatttaccc tactgctcta aagaaaagct tgatgaactt ttttatattt
     2941 gaagctgaat tctctttaat gtttgtaaaa gcaaatgcgg gggcagagga taaatattat
     3001 attgcaggcc atgtttttcg tattatttca tgcttaaatc aagtactatt tgcatgtaat
     3061 aatgcttatt gcattaacga aaagaaagct ataaaactgc ttgaaacttt tgaatataaa
     3121 cctgaaaaat atgccgaaag agttaatcat atttttgaag tactcggtct ttctcttttt
     3181 gaatgctacg acatgaccga gaaactttat aaagaagtga atgaaattgt atcggagata
     3241 aataactttt taaacgagga gagttcagat gaaagaaaac aaatatgatg ataatatatt
     3301 ttttcaaaaa tacagtcaaa tgagtcgctc gcagaaagga ctggctggtg cgggagaatg
     3361 ggagactttg aaaaagatgc tacctgattt taagggtaag cgtgtgcttg atttaggatg
     3421 cggctatgga tggcactgta ttatatatgc gatggaaaac ggtgcttcct ctgtagtagg
     3481 tgttgatatt tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat
     3541 tgaatatgaa tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat
     3601 actaagttcg cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata
     3661 taggatgctg aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc
     3721 tcatggaaca caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga
     3781 caattattat tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata
     3841 tcatagaaca ctgaccacat atctaaatac actgctttca aatagtttta taataaatca
     3901 gattgtggag ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat
     3961 gcgacgccca atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa
     4021 taaacgagga gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta
     4081 gcagaacagg atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat
     4141 atacctaaag atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg
     4201 tttatatcta atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg
     4261 gaggatatgg aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat
     4321 gattacaata aaattgatct taccttattg cccttggaag agttagataa ttacctaaag
     4381 ggcgataaat taataaaggg tctaattgat aaagattgta gaattaaaag ggacatagtt
     4441 ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc
     4501 aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta
     4561 tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg
     4621 aaggtcggca tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa
     4681 aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat
     4741 gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag
     4801 gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc
     4861 agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct
     4921 gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata
     4981 tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg
     5041 agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg
     5101 aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga
     5161 taatatatct tttaacccgt ggtgctagtt aattttaata tcctaaagaa ta
//
LOCUS       DAPBAB010000253         3970 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.146, whole genome shotgun sequence.
ACCESSION   DAPBAB010000253 DAPBAB010000000
VERSION     DAPBAB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.146"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="RD444_003223"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="RD444_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDT7550476.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD444_003224"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD444_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7550477.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1254 a          613 c          818 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAPBAB010000254         3970 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.147, whole genome shotgun sequence.
ACCESSION   DAPBAB010000254 DAPBAB010000000
VERSION     DAPBAB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.147"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="RD444_003225"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="RD444_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDT7550478.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD444_003226"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD444_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7550479.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1253 a          613 c          819 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAPBAB010000255         3276 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.159, whole genome shotgun sequence.
ACCESSION   DAPBAB010000255 DAPBAB010000000
VERSION     DAPBAB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.159"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            275..772
                     /gene="dfrG"
                     /locus_tag="RD444_003227"
     CDS             275..772
                     /gene="dfrG"
                     /locus_tag="RD444_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDT7550480.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(844..2796)
                     /locus_tag="RD444_003228"
     CDS             complement(844..2796)
                     /locus_tag="RD444_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDT7550481.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2803..3039)
                     /locus_tag="RD444_003229"
     CDS             complement(2803..3039)
                     /locus_tag="RD444_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7550482.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1015 a          566 c          538 g         1157 t
ORIGIN      
        1 taagtagttc agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt
       61 tcattttaac ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg
      121 ataattgaat gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat
      181 tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt
      241 agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat
      301 ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg
      361 ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga
      421 atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt
      481 tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa
      541 cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt
      601 tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga
      661 agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa
      721 tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg
      781 ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc caagctcttt
      841 ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct
      901 ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta
      961 agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag cgtaaatata
     1021 gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata tataacgtct
     1081 tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca tctttgcgat
     1141 ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa atcctttatc
     1201 cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc tttctccatc
     1261 tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta gataatgaga
     1321 tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat cgagcagttc
     1381 tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct tgttaacttg
     1441 gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc tataaaaggc
     1501 gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat taaccatttg
     1561 tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta aaccatcgac
     1621 aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc tattttcgct
     1681 atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat ttttacgact
     1741 aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt catccattga
     1801 aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga cctcagcaga
     1861 tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct ttgaaccttg
     1921 tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa gccacatctc
     1981 gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg
     2041 cctatacagc cactccagct tatgatagta ggttccaact ccaattccta aaatatcaca
     2101 agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa gaatggtgtt
     2161 cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag tgaacttctt
     2221 acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt tgtaaaattg
     2281 cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat gaaattgata
     2341 atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt cttcaacaac
     2401 agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc caataggatt
     2461 gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca ttttatatcg
     2521 acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa
     2581 cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt taaaagacgt
     2641 tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta ataaatcata
     2701 ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat ctacaatttc
     2761 atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca ctctatcttt
     2821 gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc attcaataaa
     2881 cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt cagtacgctc
     2941 tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt cccaatccac
     3001 agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca cccctttttg
     3061 cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa ttatttcacg
     3121 ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca acaaacgttg
     3181 atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat ttaggtttac
     3241 attttcactt tttgtactcc aaaactgaac tactta
//
LOCUS       DAPBAB010000256         2698 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.167, whole genome shotgun sequence.
ACCESSION   DAPBAB010000256 DAPBAB010000000
VERSION     DAPBAB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.167"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            87..1055
                     /locus_tag="RD444_003230"
     CDS             87..1055
                     /locus_tag="RD444_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDT7550483.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1048..2079
                     /gene="vanA"
                     /locus_tag="RD444_003231"
     CDS             1048..2079
                     /gene="vanA"
                     /locus_tag="RD444_003231"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDT7550484.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2085..2693
                     /locus_tag="RD444_003232"
     CDS             2085..2693
                     /locus_tag="RD444_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDT7550485.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          790 a          523 c          670 g          715 t
ORIGIN      
        1 tcgttcttca gagaaagatt tgacaagatt accattggca tccccgtttt atttggtgcc
       61 tttcacagaa agggttggtc ttaattatga ataacatcgg cattactgtt tatggatgtg
      121 agcaggatga ggcagatgca ttccatgctc tttcgcctcg ctttggcgtt atggcaacga
      181 taattaacgc caacgtgtcg gaatccaacg ccaaatccgc gcctttcaat caatgtatca
      241 gtgtgggaca taaatcagag atttccgcct ctattcttct tgcgctgaag agagccggtg
      301 tgaaatatat ttctacccga agcatcggct gcaatcatat agatacaact gctgctaaga
      361 gaatgggcat cactgtcgac aatgtggcgt actcgccgga tagcgttgcc gattatacta
      421 tgatgctaat tcttatggca gtacgcaacg taaaatcgat tgtgcgctct gtggaaaaac
      481 atgatttcag gttggacagc gaccgtggca aggtactcag cgacatgaca gttggtgtgg
      541 tgggaacggg ccagataggc aaagcggtta ttgagcggct gcgaggattt ggatgtaaag
      601 tgttggctta tagtcgcagc cgaagtatag aggtaaacta tgtaccgttt gatgagttgc
      661 tgcaaaatag cgatatcgtt acgcttcatg tgccgctcaa tacggatacg cactatatta
      721 tcagccacga acaaatacag agaatgaagc aaggagcatt tcttatcaat actgggcgcg
      781 gtccacttgt agatacctat gagttggtta aagcattaga aaacgggaaa ctgggcggtg
      841 ccgcattgga tgtattggaa ggagaggaag agtttttcta ctctgattgc acccaaaaac
      901 caattgataa tcaattttta cttaaacttc aaagaatgcc taacgtgata atcacaccgc
      961 atacggccta ttataccgag caagcgttgc gtgataccgt tgaaaaaacc attaaaaact
     1021 gtttggattt tgaaaggaga caggagcatg aatagaataa aagttgcaat actgtttggg
     1081 ggttgctcag aggagcatga cgtatcggta aaatctgcaa tagagatagc cgctaacatt
     1141 aataaagaaa aatacgagcc gttatacatt ggaattacga aatctggtgt atggaaaatg
     1201 tgcgaaaaac cttgcgcgga atgggaaaac gacaattgct attcagctgt actctcgccg
     1261 gataaaaaaa tgcacggatt acttgttaaa aagaaccatg aatatgaaat caaccatgtt
     1321 gatgtagcat tttcagcttt gcatggcaag tcaggtgaag atggatccat acaaggtctg
     1381 tttgaattgt ccggtatccc ttttgtaggc tgcgatattc aaagctcagc aatttgtatg
     1441 gacaaatcgt tgacatacat cgttgcgaaa aatgctggga tagctactcc cgccttttgg
     1501 gttattaata aagatgatag gccggtggca gctacgttta cctatcctgt ttttgttaag
     1561 ccggcgcgtt caggctcatc cttcggtgtg aaaaaagtca atagcgcgga cgaattggac
     1621 tacgcaattg aatcggcaag acaatatgac agcaaaatct taattgagca ggctgtttcg
     1681 ggctgtgagg tcggttgtgc ggtattggga aacagtgccg cgttagttgt tggcgaggtg
     1741 gaccaaatca ggctgcagta cggaatcttt cgtattcatc aggaagtcga gccggaaaaa
     1801 ggctctgaaa acgcagttat aaccgttccc gcagaccttt cagcagagga gcgaggacgg
     1861 atacaggaaa cggcaaaaaa aatatataaa gcgctcggct gtagaggtct agcccgtgtg
     1921 gatatgtttt tacaagataa cggccgcatt gtactgaacg aagtcaatac tctgcccggt
     1981 ttcacgtcat acagtcgtta tccccgtatg atggccgctg caggtattgc acttcccgaa
     2041 ctgattgacc gcttgatcgt attagcgtta aaggggtgat aagcatggaa ataggattta
     2101 cttttttaga tgaaatagta cacggtgttc gttgggacgc taaatatgcc acttgggata
     2161 atttcaccgg aaaaccggtt gacggttatg aagtaaatcg cattgtaggg acatacgagt
     2221 tggctgaatc gcttttgaag gcaaaagaac tggctgctac ccaagggtac ggattgcttc
     2281 tatgggacgg ttaccgtcct aagcgtgctg taaactgttt tatgcaatgg gctgcacagc
     2341 cggaaaataa cctgacaaag gaaagttatt atcccaatat tgaccgaact gagatgattt
     2401 caaaaggata cgtggcttca aaatcaagcc atagccgcgg cagtgccatt gatcttacgc
     2461 tttatcgatt agacacgggt gagcttgtac caatggggag ccgatttgat tttatggatg
     2521 aacgctctca tcatgcggca aatggaatat catgcaatga agcgcaaaat cgcagacgtt
     2581 tgcgctccat catggaaaac agtgggtttg aagcatatag cctcgaatgg tggcactatg
     2641 tattaagaga cgaaccatac cccaatagct attttgattt ccccgttaaa taaacttt
//
LOCUS       DAPBAB010000257         2236 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.175, whole genome shotgun sequence.
ACCESSION   DAPBAB010000257 DAPBAB010000000
VERSION     DAPBAB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.175"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            24..416
                     /locus_tag="RD444_003233"
     CDS             24..416
                     /locus_tag="RD444_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7550486.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..1856
                     /locus_tag="RD444_003234"
                     /note="aph(2'')-Ia"
     CDS             417..1856
                     /locus_tag="RD444_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDT7550487.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1986..>2236)
                     /locus_tag="RD444_003235"
     CDS             complement(1986..>2236)
                     /locus_tag="RD444_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7550488.1"
                     /translation="LFDSRFKTLQQNPFQYTVQGNSHNRLKSTNLIERLNQEVRRREK
                     IIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          896 a          189 c          385 g          766 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatgg tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta tggaattaaa aatattaaac
     1801 aggaatttat cgaaaatggt agaaaagaaa tttataaaag gacttataaa gattgattat
     1861 ataatatatg aaaagctatt ataaaagaca ttagtattaa atagtttaaa aaaatgaaaa
     1921 ataataaagg aagtgagtca agtccagact cctgtgtaaa atgctataca atgtttttac
     1981 catttctact tatcaaaatt gatgtatttt cttgaagaat aaatccattc atcatgtagg
     2041 tccataagaa cggctccaat taagcgattg gctgatgttt gattggggaa gatgcgaata
     2101 atcttttctc ttctgcgtac ttcttgattc agtcgttcaa ttagattggt actctttagt
     2161 cgattgtggg aatttccttg tacggtatat tgaaaggggt tctgttgcaa agttttaaat
     2221 ctactatcaa ataagg
//
LOCUS       DAPBAB010000258         1865 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.187, whole genome shotgun sequence.
ACCESSION   DAPBAB010000258 DAPBAB010000000
VERSION     DAPBAB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.187"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            <1..641
                     /gene="vanR"
                     /locus_tag="RD444_003236"
     CDS             <1..641
                     /gene="vanR"
                     /locus_tag="RD444_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDT7550489.1"
                     /translation="LYLKNENYTVFKYYTAKEALECIDKSEIDLAILDIMLPGTSGLT
                     ICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITKPFRPLELIARVKAQLRRYK
                     KFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPTEFSILRILCENKGNVVSSE
                     LLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKYIKTVWGVGYKIEK"
     gene            619..1773
                     /locus_tag="RD444_003237"
     CDS             619..1773
                     /locus_tag="RD444_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDT7550490.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          610 a          362 c          403 g          490 t
ORIGIN      
        1 aattatactt aaaaaacgag aattatacgg ttttcaaata ctataccgcc aaagaagcat
       61 tggaatgtat agacaagtct gagattgacc ttgccatatt ggacatcatg cttcccggca
      121 caagcggcct tactatctgt caaaaaataa gggacaagca cacctatccg attatcatgc
      181 tgaccgggaa agatacagag gtagataaaa ttacagggtt aacaatcggc gcggatgatt
      241 atataacgaa gccctttcgc ccactggagt taattgctcg ggtaaaggcc cagttgcgcc
      301 gatacaaaaa attcagtgga gtaaaggagc agaacgaaaa tgttatcgtc cactccggcc
      361 ttgtcattaa tgttaacacc catgagtgtt atctgaacga gaagcagtta tcccttactc
      421 ccaccgagtt ttcaatactg cgaatcctct gtgaaaacaa ggggaatgtg gttagctccg
      481 agctgctatt tcatgagata tggggcgacg aatatttcag caagagcaac aacaccatca
      541 ccgtgcatat ccggcatttg cgcgaaaaaa tgaacgacac cattgataat ccgaaatata
      601 taaaaacggt atggggggtt ggttataaaa ttgaaaaata aaaaaaacga ctattccaaa
      661 ctagaacgaa aactttacat gtatatcgtt gcaattgttg tggtagcaat tgtattcgtg
      721 ttgtatattc gttcaatgat ccgagggaaa cttggggatt ggatcttaag tattttggaa
      781 aacaaatatg acttaaatca cctggacgcg atgaaattat atcaatattc catacggaac
      841 aatatagata tctttattta tgtggcgatt gtcattagta ttcttattct atgtcgcgtc
      901 atgctttcaa aattcgcaaa atactttgac gagataaata ccggcattga tgtacttatt
      961 cagaacgaag ataaacaaat tgagctttct gcggaaatgg atgttatgga acaaaagctc
     1021 aacacattaa aacggactct ggaaaagcga gagcaggatg caaagctggc cgaacaaaga
     1081 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
     1141 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
     1201 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
     1261 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
     1321 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
     1381 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
     1441 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
     1501 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
     1561 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
     1621 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
     1681 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
     1741 atgccagact tggttgataa aaggaggtcc taagagatgt atataatttt ttaggaaaat
     1801 ctcaaggtta tctttacttt ttcttaggaa attaacaatt taatattaag aaacggctcg
     1861 ttctt
//
LOCUS       DAPBAB010000259         1504 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.195, whole genome shotgun sequence.
ACCESSION   DAPBAB010000259 DAPBAB010000000
VERSION     DAPBAB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.195"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            262..345
                     /locus_tag="RD444_003238"
                     /pseudo
     CDS             262..345
                     /locus_tag="RD444_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="RD444_003239"
     CDS             394..477
                     /locus_tag="RD444_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDT7550491.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="RD444_003240"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="RD444_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDT7550492.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAPBAB010000260         1348 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.207, whole genome shotgun sequence.
ACCESSION   DAPBAB010000260 DAPBAB010000000
VERSION     DAPBAB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.207"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            45..296
                     /locus_tag="RD444_003241"
                     /pseudo
     CDS             45..296
                     /locus_tag="RD444_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            389..1183
                     /locus_tag="RD444_003242"
                     /note="aph(3')-IIIa"
     CDS             389..1183
                     /locus_tag="RD444_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDT7550493.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          233 c          322 g          369 t
ORIGIN      
        1 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
       61 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      121 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      181 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      241 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      301 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      361 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      421 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      481 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      541 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      601 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      661 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      721 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      781 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      841 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      901 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
      961 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1021 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1081 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1141 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1201 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1261 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1321 gcctacgagg aatttgtatc gataagaa
//
LOCUS       DAPBAB010000261          922 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE_DUN_018
            SAMN36683365-rid18910023.guided.223, whole genome shotgun sequence.
ACCESSION   DAPBAB010000261 DAPBAB010000000
VERSION     DAPBAB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683365
            Sequence Read Archive: SRR25390947
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831710.1
            Genome Coverage       :: 120x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:04:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,243
            CDSs (total)                      :: 3,172
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 71
            rRNAs                             :: 1, 4, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 40 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 27 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683365-rid18910023.guided.223"
                     /isolate="VRE_DUN_018"
                     /isolation_source="Rectal screen"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Dundee"
                     /lat_lon="56.46 N 2.97 W"
                     /collection_date="2015-07"
                     /collected_by="NHS Tayside"
                     /note="genotype: vanA"
     gene            84..818
                     /locus_tag="RD444_003243"
     CDS             84..818
                     /locus_tag="RD444_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HDT7550494.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          378 a          125 c          124 g          295 t
ORIGIN      
        1 atcaacacag ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat
       61 aaacctcata ggagtgggtt ataatgaaca aaaaaaatat aaaagatagt caaaacttta
      121 ttacttcaaa gcatcatata aatgaaattt tgagaaatgt acatttaaat acaaatgata
      181 atattattga gattggttca gggaaaggtc atttcacgtt tgaattagct aaaaggtgta
      241 attatgtaac cgccattgaa atagatccta aattatgtag gataactaaa aacaaactta
      301 ttgaatatga gaacttccag gttatcaata aagatatttt acaatttaag tttcctaaaa
      361 ataagtcata taagatatat ggaaatatac cctacaatat aagtacagat ataattcgaa
      421 aaattgtttt tgaaagcaca gctacagaaa gttatttaat agtggaatat ggatttgcta
      481 aaaggttgct aaatacaaat cgttcactag cactattttt aatgacagaa gttgatatat
      541 ccatattaag taaaatccct agagaatact ttcatccaaa acctagagtt aatagctcgt
      601 taattgtatt aaaaagacac ccttcaaaaa tatcactcaa agatagaaaa caatatgaaa
      661 attttgttat gaaatgggtt aacaaagaat acataaaact attttccaaa aaccaatttt
      721 atcaagcctt aaaatatgca agaattgacg atttaaacaa tattagcttt gaacaattct
      781 tgtctctttt caatagctat aaattattta atagataagt taaggggtgc ataaactgca
      841 tcccttaact tatcttattt tgcttaaaca atcttggccc gtaaactgca taactcatat
      901 gtaaatcgct cctttttagg tg
//
